LOCUS       DAOTKS010000001        15419 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_4273_length_15419_cov_5.274993, whole genome shotgun sequence.
ACCESSION   DAOTKS010000001 DAOTKS010000000
VERSION     DAOTKS010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 15419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15419
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4273_length_15419_cov_5.274993"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /gene="amrS"
                     /locus_tag="P5236_00005"
     CDS             complement(<1..949)
                     /gene="amrS"
                     /locus_tag="P5236_00005"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HRU62783.1"
                     /translation="MELTKWTRQALYQEQLQDKIVRCHLCPHNCTIRNEKYGICRTRV
                     NIDGTLYTVAYGNPCSANIDPIEKKPLFHFLPGSKIFSIATAGCNFKCLNCQNWEISQ
                     SSPQELSHYDLPPEAVIELAAKNNITSIAFTYTEPTVFYEYVYDTAKLAHEKNIKTVI
                     VSNGYINDKPLLELCQYLDAANIDLKCIDDQIYRNLTGGKLQPVLNSLQILQKQGVWL
                     EITNLIVPTYSDAPEMIENMCKWLVNNGFENTPLHFSRFFPANQLPDLPPTPIKTLLA
                     AREIAQNAGMKYIYVGNVPAMGYENTFCPSCKKLLIERVG"
     gene            1565..3514
                     /gene="dnaG"
                     /locus_tag="P5236_00010"
                     /pseudo
     CDS             1565..3514
                     /gene="dnaG"
                     /locus_tag="P5236_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854905.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
     gene            3536..4171
                     /locus_tag="P5236_00015"
     CDS             3536..4171
                     /locus_tag="P5236_00015"
                     /inference="COORDINATES: protein motif:HMM:NF023096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HRU62784.1"
                     /translation="MTYQELIQQPDYVQLASVPHSEIKSFIKNEISEMPKWARIANMY
                     QLIGLLAFMLGLFKAFMPFFVYREVNYLIWLGLGTVFTFTFLIILHEFIHAIAYWCIG
                     SRHLSFGFQLKKFLFYVECDREVVNYQQFKKIALAPTIAVTILSLLGMILFYQQQLFY
                     FFLPIFSLHNLFAAGDFGLLCFFQNRPDWEIVTFDVKEEQKTYFYGKKKQN"
     gene            4260..5030
                     /locus_tag="P5236_00020"
     CDS             4260..5030
                     /locus_tag="P5236_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRU62785.1"
                     /translation="MILFDKIVFGPVHSRRLGISLGINLLPIDAKICTYDCLYCECGF
                     NTRMKKSPLPKREEVSAALEAELQKMISLEKIPDIITFAGNGEPTIHPDFENIIEDTL
                     QLRDIYCPLAKVGVLSNATEIHKPQVFRALNKVDDNILKFDTAFDHTLQLLNRPTQKY
                     ITVKWLIEHLKKFEGKMIIQTLFIRGEYNGEKVDNTTDEEINAWLQALREMNATQIMI
                     YTIDRETPLKNLEKIPLNELNTIAEKARNYGFDVSIST"
     gene            5102..6046
                     /locus_tag="P5236_00025"
     CDS             5102..6046
                     /locus_tag="P5236_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU62786.1"
                     /translation="MKILVCPLNWGLGHATRCIPIIQKLLAEKNEVIIAANGFPLQLL
                     RQEIPSLRWIEFPSYKVQYSSKNSQIATILKWLPFILYGIVKEHYWLKKIIAQEHFDK
                     VISDNRFGLWNRKIHSIYITHQLMIKMPRNNKMLEWIAWKLHRFIIQNYNECWIPDTE
                     NAPGLSGDLAHKYPLPNNAKFIGPISRFQNLPYIPSQKKFEVVVIVSGLEPQRTLFEQ
                     KMIQLHKKNPYQTLIIQGQPQQKITTQKIGNITLISHLPAIEMASLLKNADKIFARSG
                     YSTIMDLFTLNCLSKAVFFPTPGQTEQIYLSEIHSTNT"
     gene            6184..7317
                     /gene="nspC"
                     /locus_tag="P5236_00030"
     CDS             6184..7317
                     /gene="nspC"
                     /locus_tag="P5236_00030"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HRU62787.1"
                     /translation="MIDYSQIPSPCFVLDEVAFRKNLALIKSVKDRSGAEIILAFKAF
                     AMWSVFPIVREYISGAAASSLWEARLAFEEMKTKAHTYSPAYTEKEFPEILNYSSHIT
                     FNSLSQFYKFHSQTKEKNISCGLRINPEFSPVTTNLYNPCLPGSRLGILAQQLGEKLP
                     DGIEGLHFHVLCESDAEDSAKLLQVIEDKFGKYFSQLKWLNLGGGHLMTKKNYNIELL
                     VSVLKSFKEKYPHIQLILEPGSAFAWQTGVLVATVTDIVENKGIKTALLNVSFSCHMP
                     DCLEMPYKPTIIGATDEIPGKPTYRMGGNSCLAGDFCGSWSFDKELKVGDRIVFDDMI
                     HYTIVKTTMFNGVTHPSISLWTKDNQFELIREFTYEDYKNKLS"
     gene            7349..7723
                     /locus_tag="P5236_00035"
     CDS             7349..7723
                     /locus_tag="P5236_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009040396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HRU62788.1"
                     /translation="MKIIGNILWLILGGLVIAAEYLISSLLLMVTIVGIPFGIQTLKL
                     SVLALWPFGSDIQSTSAETNFLFVLMNIIWLLVGGIWIALTHIVFGILCAITIIGIPF
                     AKQHFKLAALALTPFGKEISTI"
     gene            complement(7804..10821)
                     /gene="secDF"
                     /locus_tag="P5236_00040"
     CDS             complement(7804..10821)
                     /gene="secDF"
                     /locus_tag="P5236_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HRU62789.1"
                     /translation="MQNKGLVKVFFIALSLVCLYYLSFTIVSSHYSRKAKQYAGGDPL
                     KEYAYLDSLATKKVWLGYTLKECREREINLGLDLKGGMNVVLEISVPDIIRALSNYNT
                     TPNFVKALEIATERQKNSQADFLDLFVQAYKELDPHAKLASIFSTYQLKDKISLNSTN
                     EEVITVLRQEINDAISNSFNVLRTRIDRFGVVQPNIQKLSTTGRILIELPGIKEPERV
                     RKLLQGSANLEFWETYDLNEIFNNLMEANRILASIQVIDNQATESKDTVQTKITTTQE
                     KQSEVDSLKAILAKKAEITSDSLKMLEQRKKENPLFSVIQLNTINGQIGRGPLVGLVH
                     SKDTALVNDYFSLPQVKEVLPRDLSFRWSVKPRDEKGEWFELIAIKVTNRDGRAPLGG
                     NVITDARVDFGQTSAYANVSMSMNAEGSKIWARLTKDNIGKSIAIVLDGYVYSYPTVQ
                     NEIKGGQSQITGNFTIEEAKDLANVLKSGKMPAPARIVQEDVVGPSLGQEAIQSGFIS
                     FIIAFLMVLIYMIFFYGWIPGLVADIALFANVFFLLGILASFSAVLTLPGIAGIVLTL
                     GMAVDANVITYERIREEMRAGKNMKRAVQEGYKNALSAIIDGQATTLITGIILAIFGT
                     GPIKGFATTLIIGIITSFITAVFLSRLIIEWYVSRDNAKEISFTTNLTKNFLQNTRIN
                     FIGIRKYAYTFSGICIFIALISFAVRGFDMGIDFTGGRNYIVRFDQPVQTDHVRSLLE
                     NSFEEAGVQVITIGSENQVRISTKYKIDDNSENVENEIEQRLYTGLKPLLKENVTQEQ
                     FVQDYIKSSQKVGPTVASDIRNAAIWSIVFALLAIGLYILIRFHELGYALGAVLSLVH
                     DAIITIGLFSLFYSVMPFSMEINLSFIAAILTVLGYSINDTVIIYDRIREYIKLYPKR
                     DRLSIMNNAINATLSRTFNTAMTTLLVLIIIFIFGGETIRGFIFALLFGIGIGTYSSV
                     FVASPLTYEYYKWKTKREAKKIGGTKKVIVKTT"
     gene            11093..11164
                     /locus_tag="P5236_00045"
     tRNA            11093..11164
                     /locus_tag="P5236_00045"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11125..11127,aa:Met,seq:cat)
     gene            11632..12189
                     /locus_tag="P5236_00050"
     CDS             11632..12189
                     /locus_tag="P5236_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU62790.1"
                     /translation="MVFRFTILSDEADDFIRVIDMDAEATFLDLHNAILDSVNFKKNE
                     ITSFFLCSDEWEKEQEITLIEMETSSEYDNLVMDKVKLGDLLTDEKQKLLYVFDMISD
                     RSFFIELSEMIPHKKLSKPVCRISKGNPPEQMLMENDLSTIDKINLDENFYGDESFDI
                     EELDSEGFEDLDFGEGNFSEDDLNY"
     gene            12272..13198
                     /gene="miaA"
                     /locus_tag="P5236_00055"
     CDS             12272..13198
                     /gene="miaA"
                     /locus_tag="P5236_00055"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRU62791.1"
                     /translation="MNQSPTLLVLLGPTGVGKTEISLRLAQHFKSPIISADSRQIYKE
                     LKIGTASPSEEQLSTIKHYFIASHTIFENYSAGQFEQEVLLLLNELFQTHQIVLMVGG
                     SMLYIDAVCKGLDNIPSIDAEIRNFWQNKFKDQGLEYLQQELKKLDPVYYQQVDLHNY
                     KRILHALEICSITGKPFSELRTGKAQKRPFHILKIGLTRPRAELYERINERVDQMIKN
                     GLLEEAMQYYPYRNLNPLNTVGYKEIYDYLDGKYNLEKAIEKIKQNTRHYAKRQLTWF
                     KHDEDIQWFHPEEEEKIIDYVTKNLPLNDCLS"
     gene            13182..14201
                     /gene="murB"
                     /locus_tag="P5236_00060"
     CDS             13182..14201
                     /gene="murB"
                     /locus_tag="P5236_00060"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013265773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRU62792.1"
                     /translation="MIVYHNFSLLPYNTFQLDIKADSFIEFDSVEELKNILVSPLLLS
                     SPNIMIIGRGSNLLFLDDFKGTILHSGIKFIKKIDENNDFVFLEVGSGIIWDDFVQFC
                     VENDYQGIENLSGIPGEVGAAAVQNIGAYGVEIQEMIEKVNVVEIHSAKIHEFANADC
                     QYGYRKSIFKKELKGKLVVTSVIFRLNKKSTYKLEYEGLEKEVHAKGEINLKNIRQAI
                     LDIRSRKLPDYKEFGNAGSFFVNPIISKENFQKLQKSYPNIPHYTISEKEEKIPAAWL
                     IEQCGWKGKQIGQVKIHEHQPLILINCGNASGKEIAKVANQIQASVKDKFQIELQPEV
                     NYIYS"
     gene            complement(14208..15257)
                     /gene="mltG"
                     /locus_tag="P5236_00065"
     CDS             complement(14208..15257)
                     /gene="mltG"
                     /locus_tag="P5236_00065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRU62793.1"
                     /translation="MSDSFFTKKTLKKIFKWTTIFISIILVYFLYILFFPNIFPPKKT
                     RTFLYIRENSQLKEVVDSLSKNTKVFDVYSFYVASKLLDYESHIKSGRYELQRGMNNF
                     ELIRKLRSGRQTPVLLIINNIRTKEDLAHRLSMQLMVDSASIIQLLNDSTFLAEYGLN
                     PYNSISIFIPNTYEVFWNISPEKLFARMYNEYNRFWNEERRKKAAAIPLTPLEVITLA
                     SIVEEETNVESERPIVAGVYINRLKKGMPLQADPTLKFAAGNFALRRISGIYLKIDSP
                     YNTYKYKGLPPGPIRLPSQKSIDAVLNYTPHNYLYMCAKETLNGEHNFASNWKEHLQN
                     AKKYQQALNRMGIFE"
BASE COUNT         5218 a         2466 c         2529 g         5206 t
ORIGIN      
        1 agccaacccg ttctatcagc aattttttgc aggaaggaca gaaggtattt tcataaccca
       61 tggcaggtac gtttcctaca taaatatatt tcattcctgc attttgggca atttcacgtg
      121 cggcaagcaa tgtttttatg ggagttggcg gcaaatcggg caactgattg gcagggaaaa
      181 atcgactaaa atggagtggt gtgttttcaa agccattgtt aaccagccat ttacacatat
      241 tctcaatcat ttctggggca tcagaataag tgggcacaat cagattggta atttccagcc
      301 aaacaccttg cttttgtaga atttgtaaag agtttaaaac aggttggagt ttgcctcctg
      361 ttaaatttct ataaatttga tcgtcgatgc attttaaatc tatattagca gcgtctaaat
      421 attggcataa ctcaagtaaa ggtttatcgt tgatataacc attggaaaca atcaccgttt
      481 taatgttctt ttcatgtgcc aattttgccg tgtcgtaaac atattcgtaa aataccgtcg
      541 gttctgtata ggtgaaggca atgctggtaa tgttgttttt tgctgccaat tctattacgg
      601 cttctggtgg caaatcataa tgactcaatt cctgcggtga actttgagag atttcccaat
      661 tttgacaatt caaacatttg aagttgcatc cagccgtagc aatagaaaaa atttttgatc
      721 caggtaaaaa atggaacaaa ggtttttttt caatgggatc aatatttgct gagcaaggat
      781 tgccgtaagc tacggtataa agtgttccgt ctatgtttac tcttgttcgg caaataccgt
      841 atttttcatt tcttatggtg caattatggg ggcaaaggtg gcagcgaact attttatctt
      901 gtaattgttc ctgatataat gcctgacgtg tccatttggt taattccatg atttattaat
      961 ttttacttgc tgaattctat tacacaaata tattcttttt gaaaataatt agaaaatatt
     1021 tcctttcaaa atgaatgatt tttcggattt tattttgaaa cttatagtta gctgatttat
     1081 agggataaaa tcagtcgaaa tattatgatt ttttccaatt ttaattaaaa aatttttaaa
     1141 catttgtgta atcaatcgta aactcgatac aataataaga tgataaggtt tggtggagtt
     1201 aggaagttaa cggttttcga taaggttttt atgacaaaat attggtttag caaaacgttt
     1261 gattttatca gcggttaaaa tcaggaaatt atatttatta tgcgacgtag ccagaaggtt
     1321 acgtcaaata atgggataca gagatttttt cttcattcgt tcaaaatcta ctcgtacctc
     1381 gcacctcgca ccttgtatct ttcaaactct tgattgccaa ctaaatattg gttaattttc
     1441 attttttaga attttactat aatttgtaga ataaattcat tagctttgta ataataaacc
     1501 tatagagcaa tactttaact aatcatttct cttcctttgt tttaatttag tttttttgca
     1561 agacatgatt gatcaagcta ccattgatag aattattcag gctgcaccta ttcaagaagt
     1621 tatttcggaa tatgttacct taaaacgacg aggagccaat ttaattggat tgtgtccttt
     1681 tcataacgaa aaaacaccct cctttacagt ttcgcccagc aagggaattt ttaaatgttt
     1741 cggatgtaac aaaggtggca acgtggttca ttttattatg gaacacgaac aaatgacgta
     1801 ttatgaagct cttaaatttc tggcaaaaaa atacaacata gaagttcagg aaaaagaact
     1861 tactcctgag gaaatagaag ctcataatga acgtgaaagt ttattgttgg tcaacgattt
     1921 tgccaataaa tatttcatga acattctgca tcatcacaac gaggggagga ctgtcggtct
     1981 tagttatttt caggaacgcg gttttcgcga agatattatt caaaaatttc aattgggtta
     2041 ttgtctcgat tctcgcgatg cttttactta agctgcttta aaagaaggtt ataagaaaga
     2101 attcctcatc aaaacgggtt tgacgataga atcagaaaat aattattttt tagatcgttt
     2161 tcgaggaaga gtaatttttc ccattcattc attatcgggt aaaataattg cttttggagg
     2221 aagagtttta aaaaaggagg aaaaagctgc caaatacctt aattctcctg aatcggaaat
     2281 ttatcataaa agtaatgaac tgtatggtct ctattttgca aaacaagcca ttgtccgtga
     2341 aaacaggtgt tatttggtag aaggttatgc agatgtaatt tcattttttc aaagcggaat
     2401 tgaaaatgta gtagcttcgt cgggtacttc gctgacgcct gggcaaattc atcttatcca
     2461 ccgcttcaca gaaaatgtaa cagtaattta tgatggcgat gctgctggta tcaaagcttc
     2521 attaagagga atagaccttc ttttggaaga aggcttgaac attaaagtag tgttgcttcc
     2581 cgaaggagaa gaccctgact catttgccca taaaaacaat gcttcggatg taatagcatt
     2641 tatccaacaa aactcagttg actttattcg ctttaaaact aatttattat tgaatgacgc
     2701 tggtaatgac ccttttaaac gtgccaattt gattatggat attgtgcaaa gcattgctat
     2761 tatcccaaat ccgatcgtaa gagctgaata tgtgaaggaa tgcagcaact tgttgtcggt
     2821 tgaagaacaa atgctttacc atgaaatcaa taaattgatt aaaaagcaaa gagaaaaaaa
     2881 cataaaatct accagtctca tttccggcga ttcaactact aaaatttcgt cttctcaacc
     2941 accaattgat gtaaatgagc aaaaatttga tgaagaagaa cgcaatattc ttcaagtctt
     3001 aatcaaatat gggaaatggg tattgtatta tgaagacata gaaaatacat tacctgtcac
     3061 ggttgaagat tatattttaa aagaattaga acaagatcaa cttcacttta acaatgctct
     3121 ttatgctctt gtgctggatg aatatcataa aaatagtcaa caggaaaact ttaatgctga
     3181 aaattatttt cttcatcatc ccaatagtga aattagtaaa ttagtagcca atttgatttc
     3241 ccagaaatat acacttagca aaatttattc aaaagtcaaa aaaattgatg ctgaatcaga
     3301 aaaattaagg gactatgtgg aacgtgtgat tttagaatat aaaaacagca tcgttgtatc
     3361 tcaattgcgg gaaaaattga tagaaatgaa aaaagcaacg gaagacaaaa acgaagaact
     3421 tgttaaacaa ttaatgaaag aaattgagca actggaagaa ataaaaaatc aattgtcaaa
     3481 aaatttgggt gaacgcatta ttattaaaat ttaactatta ttacgctgaa ataaaatgac
     3541 ttaccaggaa ttaatccaac aacccgatta cgttcaactt gcctctgttc ctcatagtga
     3601 aattaaaagt tttataaaaa atgaaataag cgaaatgcct aagtgggcac gcattgcaaa
     3661 tatgtatcag ctaattggtt tacttgcttt tatgcttggt ttgttcaaag cttttatgcc
     3721 tttttttgtt tatcgtgaag ttaattattt aatctggctt ggtttgggaa ctgtttttac
     3781 ctttactttc ctgatcattt tgcatgaatt tattcacgcc attgcttatt ggtgcattgg
     3841 cagccgtcat ttgtcctttg gttttcaact aaaaaaattt ttgttttacg tcgaatgcga
     3901 tagagaagtt gttaattatc aacaatttaa gaagatagct ctggctccta ctattgctgt
     3961 taccatttta tcactgttag gaatgatttt attttatcaa caacaacttt tttatttttt
     4021 tcttcctatt ttctcgctcc ataatctttt tgcagcagga gattttggct tgttatgttt
     4081 ttttcaaaat cgacctgatt gggaaattgt aactttcgat gttaaagaag aacaaaaaac
     4141 ttatttttat ggcaagaaaa aacaaaatta aaattaaaaa ttaagcagtc gctgataaac
     4201 caattcttct tattatttgt ttgctatcat ctgtttttaa caaatattca attagtaaaa
     4261 tgatcttatt tgataaaata gtttttggtc ccgtacatag cagaaggtta ggtatttcgc
     4321 tgggaattaa tttattacct attgatgcaa aaatttgtac ttacgattgt ctctattgtg
     4381 aatgtggctt caatacaaga atgaaaaaat cccctcttcc aaaaagagaa gaagtgtcgg
     4441 ctgcacttga agctgaattg caaaaaatga tttcactgga gaaaattcct gatatcataa
     4501 cttttgcagg aaatggtgaa cctaccattc atcctgactt tgaaaacata attgaagata
     4561 ctttgcaact tcgtgatatt tattgtcccc ttgcaaaagt gggagtttta tctaacgcaa
     4621 ctgaaattca taagccacaa gttttcagag ctttaaataa agtagatgat aacattttga
     4681 aatttgatac agccttcgac catactttgc aattgctgaa ccgacccaca caaaaatata
     4741 ttacagttaa atggctgatt gaacatttaa aaaaatttga agggaaaatg attattcaaa
     4801 ctctttttat aagaggtgaa tataacggag aaaaagtgga taatactaca gatgaagaga
     4861 ttaatgcctg gttacaagct cttcgtgaaa tgaatgcaac tcagataatg atttacacta
     4921 tcgatagaga aacacctcta aaaaatcttg aaaaaattcc tttaaatgaa ttaaatacta
     4981 ttgccgaaaa agctcgtaat tatggttttg atgtttctat ttccacataa tttttaaaaa
     5041 aacaagctgg tttattattg ctttaataaa aaaacgcgaa aatacaccac tccagattgt
     5101 tatgaaaatt ttggtttgtc ctttaaattg gggattaggt catgctaccc gatgcatacc
     5161 gattattcaa aaattgcttg ctgaaaaaaa tgaggtgatc attgcagcaa atggttttcc
     5221 acttcaactg ttacgacaag aaattccttc tcttcgatgg atagaatttc cctcctacaa
     5281 ggttcaatac agttctaaaa attctcaaat cgcaactatc ctcaaatggc taccttttat
     5341 tttatatgga attgtcaaag aacattactg gttaaagaaa ataatagctc aagaacattt
     5401 tgataaggtg atttctgata atcgttttgg tttatggaat cggaaaattc attccattta
     5461 tataactcat cagctcatga taaaaatgcc tcgaaacaac aaaatgttag aatggatagc
     5521 gtggaaattg catcgtttca ttattcaaaa ttacaacgaa tgctggattc ctgatactga
     5581 aaatgctcca ggcttatcag gcgatctggc tcataaatat cctcttccta ataatgcaaa
     5641 atttattggt ccaatttccc gttttcaaaa tttaccatat attccttctc aaaaaaaatt
     5701 tgaagtagtt gtaattgttt caggattgga accgcaaaga actttatttg aacaaaaaat
     5761 gattcaactt cacaaaaaaa atccttatca aacattgata attcaaggac aaccacagca
     5821 aaaaattacg acccagaaaa ttggcaatat tacccttatt tctcatcttc ctgctattga
     5881 aatggcaagt ctgttgaaaa atgctgataa aatttttgct cgttctggtt attccactat
     5941 tatggattta tttacactga attgtttgtc aaaagcagtt tttttcccta caccaggaca
     6001 aacagaacaa atatatctat ctgaaattca ttccactaac acataaattg ttttttcttt
     6061 attttcaaaa tttttttgaa ctaaaaaaat tgttactact tttgtagaaa acgccaatgt
     6121 ttttattttt atagcttaat ttctaattaa actctttttc attattcata aatatatctt
     6181 tcaatgatag attattcaca gattccttct ccttgttttg tgttggatga agtcgcattc
     6241 agaaaaaatc tggcattgat aaagtctgta aaagatcgta gtggagctga aattattttg
     6301 gcattcaaag catttgccat gtggagtgta tttccaattg ttcgagaata tatttcggga
     6361 gcggcagcca gctcattgtg ggaagcccgt cttgcttttg aagaaatgaa aacaaaagcc
     6421 catacttatt cgccagctta tacagaaaaa gaatttcctg aaattttaaa ttatagcagt
     6481 catattactt ttaattcatt atcacaattt tacaagtttc attctcagac aaaagaaaaa
     6541 aatatttcct gcggtttacg cattaaccct gagttttcac ctgtaaccac aaatttatat
     6601 aacccttgtt taccgggttc aaggctagga atacttgctc aacaattggg agaaaaatta
     6661 ccagatggaa ttgaaggttt gcattttcat gttttatgcg aatctgatgc agaagattcg
     6721 gcaaaattgc ttcaagtgat agaagataaa tttggtaaat atttttcaca actgaaatgg
     6781 ttgaatttgg gcggcggtca tctcatgaca aaaaaaaatt ataatataga gcttcttgtt
     6841 tctgttctga aaagtttcaa ggaaaaatat cctcacattc agcttattct cgaaccagga
     6901 agtgcatttg cctggcaaac aggtgtttta gtagccaccg ttacagatat tgtagaaaat
     6961 aaaggcatca aaacagctct cttgaatgtt tcattttctt gtcacatgcc tgattgtctg
     7021 gaaatgccat ataaaccgac aatcataggt gcaacagatg aaattccagg taaaccaact
     7081 tatcgcatgg gaggaaacag ttgtttggca ggtgatttct gcggttcgtg gtcgtttgat
     7141 aaagaattaa aagttggtga ccgcattgtt ttcgacgata tgattcacta tacaatagta
     7201 aaaacgacga tgttcaatgg agtaacacat cccagtataa gtttatggac aaaagacaat
     7261 caattcgaac ttatcagaga atttacttac gaagattata agaataaatt atcttgattt
     7321 agaattactt ataaataatc aaaatgcaat gaaaataatt ggaaacattc tgtggttaat
     7381 tttgggagga ttggttatcg ctgctgaata tctcatttcc agcttattat tgatggtaac
     7441 catcgttggc attccttttg gaattcaaac attaaaatta tccgttttag ctttgtggcc
     7501 ctttggaagc gatattcaat caacctctgc tgaaaccaat tttttatttg tcctgatgaa
     7561 tataatctgg ttactggttg gtggcatttg gattgcttta acgcatatcg tttttggtat
     7621 tttatgtgcc attactatta ttggtattcc ctttgcaaaa cagcatttta aactcgcggc
     7681 attagcactt acaccctttg gaaaagaaat ttctacaatt taaaatactg ttgtaaattt
     7741 ctatctattt ttcattaaaa aaagccccat caagattttc tcaatagggc tttttagcat
     7801 ttattaagtc gttttgacaa ttaccttttt tgttccaccg attttttttg cttctctttt
     7861 tgttttccat ttataatatt cgtatgtaag aggagaagca acaaaaacag aagaataagt
     7921 tcctattcca ataccaaata ataaagcaaa gataaatcca cgaatggttt caccaccaaa
     7981 aatgaatata atgatcaata ccaataatgt agtcatagca gtattaaaag ttctgctcaa
     8041 tgttgcattg atggcattgt tcattatgct taatctatct cgtttaggat ataatttgat
     8101 gtattctctg attcggtcat aaataattac ggtatcattt atagaatatc ctaaaactgt
     8161 caagattgcc gctatgaatg ataagttaat ttccatcgag aagggcataa cactgtagaa
     8221 taatgaaaat agtcctattg tgataatagc atcatgaacc agggacaaca cagctcctaa
     8281 tgcatatcct aattcatgga aacgtattaa aatatacaaa cctatcgcta ataaagcgaa
     8341 aacaatcgac caaatggcag cgtttctgat atcactcgct accgtaggac caaccttttg
     8401 agaactttta atataatctt gaacaaattg ttcttgagtt acattttctt ttaacaaagg
     8461 ttttaaacca gtgtacaatc gttgttcaat ctcattttca acattttcag agttatcatc
     8521 aattttgtat ttagtagaaa tgcgcacttg attttctgaa ccaattgtga tgacttgtac
     8581 gccagcttcc tcaaaagaat tttccaataa agaacgcaca tggtcggttt gcacaggctg
     8641 atcgaatcgc acaatgtaat ttcgtccgcc tgtaaaatca atacccatat cgaaacctct
     8701 aactgcaaaa gaaattaaag caataaatat acagatacct gagaaagtat aagcatattt
     8761 ccgtatccca ataaaattaa tacgtgtatt ctggagaaag tttttagtaa ggttggtagt
     8821 aaatgaaatt tccttagcat tatcgcgact tacataccat tcaattatta aacgagacaa
     8881 gaacactgct gttatgaaag aagtaataat accaataatt aaagttgtgg cgaagccttt
     8941 aataggtcca gttccgaaaa ttgcaagaat aatacctgta attaaagttg ttgcttgccc
     9001 atcaataatt gctgaaagtg catttttgta accttcctgt acggctcgtt tcatgttttt
     9061 ccctgctcgc atttcttctc tgatacgttc ataggtaatt acattggcat caaccgccat
     9121 acccaatgtt aacacaatac ctgctatacc aggtaatgtt aataccgccg aaaaggaagc
     9181 taaaattccc agaaggaaaa aaacattggc aaacaaggca atatcggcta ctaaaccagg
     9241 aatccatcca taaaagaata tcatataaat caataccatc aaaaaagcga tgataaatga
     9301 aatgaagccg ctctgaatag cttcttgtcc taatgatggt cccactacat cttcttgaac
     9361 tatacgagcg ggtgcaggca ttttacctga ctttaataca tttgctaaat cttttgcttc
     9421 ttcaatggta aaattaccgg taatttgaga ctggcctcct ttaatttcat tttgtacagt
     9481 agggtaggag tatacataac catccagcac aatggctatt gatttaccaa tattatcttt
     9541 tgtcaatcga gcccaaattt tggaaccttc agcattcatt gacatgctaa cattggcata
     9601 agctgatgtt tgaccgaaat caactcgagc atcggtaata acattacctc ctaaaggagc
     9661 ccgtccatct cgattagtaa cttttatagc aattaattca aaccattcgc ctttttcatc
     9721 gcgaggtttg acactccaac gaaaactcaa atcacgcggc aatacttctt taacttgagg
     9781 aagactaaaa taatcattca ccaaagctgt atctttgcta tgaaccaatc caacaagggg
     9841 acctcgtccg atctgaccat tgatagtgtt gagttgtatt acagaaaata aaggattttc
     9901 tttttttctt tgttccaaca ttttaagtga atcagaagtt atttcagctt tttttgccaa
     9961 aatagctttc aaactatcta cttcagattg tttttcctgt gtcgtagtaa tctttgtttg
    10021 aaccgtatct ttgctttcag ttgcttgatt atcaatcact tgaatacttg ctaaaatacg
    10081 attagcttcc attaaattat taaaaatctc atttaaatca taagtttccc aaaattccaa
    10141 attagctgaa ccttgtaaaa gtttgcgaac acgttcgggt tcttttatcc ctggcaattc
    10201 tattaaaatt cttcccgtgg ttgacagttt ttgaatattg ggttgaacca ccccaaaacg
    10261 atcgatacga gttcttaaaa cattaaatga attgctaatg gcatcattaa tttcctgtct
    10321 taatactgta attacttcct cattggtaga atttaaggag attttatctt tcaattgata
    10381 ggtgctaaaa atagaagcca gtttagcatg agggtcgagt tctttatatg cttgtacgaa
    10441 taagtccaga aaatctgctt gactgttttt ctgtcgttca gtagctattt ctaaggcttt
    10501 tacaaaattt ggagtcgtat tgtaattaga taaagcacgt ataatatccg ggacagatat
    10561 ttcaagtacc acattcatgc caccctttaa atcaagtcct aaatttattt ccctttctcg
    10621 acattccttt aaagtataac ccaaccaaac tttttttgta gctaaagaat caagataagc
    10681 atattctttt agggggtcac cacctgcata ttgtttcgct tttcttgaat agtgactact
    10741 tacaatagta aaggataaat aatacaaaca tactaacgac aaggcgataa aaaaaacctt
    10801 aacaagtccc ttattttgca tttgacaatt attttaagtt taaaagtaag tatagaatac
    10861 aaaatttaac gttgcaaata tactatattt tgaagtagta aaaaaataac atttaaaata
    10921 atttagttct ctattgtatt tcaaaataat ctgtgaagat gtaaaatcac aatgatttag
    10981 gacaacaaaa aatttaaaaa catttatagc gtttcaaagt gattattttt gtcaaagaaa
    11041 gaatattcta ttgttattta caaaataaaa tattatcttt gcaaaataaa atcgcggagt
    11101 ggagcagtgg tagctcgttg ggctcataac ccaaaggtcg ttcgttcgaa tcgagcctcc
    11161 gcaactttta aaggagaaat attgcaaaat gtttcttctt ttttgtttga tctttgcttg
    11221 attttttttc tttgagggag aaatacaatc tttttctatt ttagatagaa aaaatttgtg
    11281 atttttcaga aaatttgagt gaatttcatt tagaatgtag ccatttgatt attagcaaat
    11341 aatgtcagaa agtttatgtt tttatgtttg ttgatacgat ggccaagaag tttttcaaaa
    11401 tacacttgta caatgaggtg ttttgtttcg ttgtattttt tttgctttaa agacagataa
    11461 tttttttggt aatttacaac ataacttatt aacaattaac ttattagtct gttagtaatg
    11521 gttcattttt gaaatttttt atacgttggt cttaggataa aattacagct atttcatact
    11581 ctcaaaaaaa atattaattt tacaggcgta attataatct ttaataataa tatggttttt
    11641 cgatttacaa ttttatctga cgaagcagat gattttatca gagtaattga tatggatgca
    11701 gaagcaactt ttctggatct gcataatgcc atacttgatt cagtaaattt caaaaaaaat
    11761 gaaatcactt ctttctttct gtgctcagat gaatgggaaa aagagcagga aattaccttg
    11821 attgagatgg agacttcttc agaatatgat aacttagtaa tggataaagt aaaattagga
    11881 gatttgctga ccgacgaaaa gcaaaaacta ctttatgtct ttgatatgat ttctgacaga
    11941 agtttcttta ttgaattaag tgaaatgatt cctcataaaa aattatcaaa acctgtttgc
    12001 agaatttcaa aaggaaatcc gcccgaacaa atgctcatgg aaaatgatct ttctactatt
    12061 gataaaataa acttagatga gaatttttat ggcgatgaat catttgatat agaggaactg
    12121 gacagtgaag gatttgaaga tctcgatttt ggagaaggta atttttctga agacgactta
    12181 aattattaat acttagaatt atctattggg aacagaaaat aattgttttt ttattttctg
    12241 tttttttatt tgagttaata tcccttttca aatgaaccaa tcacccactt tattagtttt
    12301 gttaggacca acaggagttg ggaaaactga aataagtctt cgattggctc aacattttaa
    12361 aagtcccatt atttctgctg attcacgcca aatttataag gaactgaaaa tcggtactgc
    12421 ttctccttca gaagaacaac tctcaactat aaaacattat tttattgctt ctcacacgat
    12481 ttttgaaaat tatagcgccg gacaatttga acaagaagta cttttattat taaatgagct
    12541 atttcaaacc catcagattg tcttaatggt tggtggatca atgctttata ttgatgccgt
    12601 atgtaaaggg ctggataata ttccttctat tgatgcagaa atacgaaatt tttggcaaaa
    12661 caaatttaaa gatcaaggac ttgaatatct ccagcaagaa ttaaagaaat tagatcctgt
    12721 ttattatcag caagtagatt tacataatta caaacgcatt ttacatgcat tggaaatatg
    12781 ttctataact ggaaaacctt tttctgaact tcgaactggt aaagctcaaa aaagaccgtt
    12841 tcatattctc aaaattggtc taacacgtcc acgagcagaa ttgtacgaac gtatcaatga
    12901 aagagtggat caaatgataa aaaatggttt gttggaagaa gctatgcaat attatcctta
    12961 cagaaatcta aatcccttga atacagtagg atataaagaa atttacgatt atttggatgg
    13021 aaaatacaat ctggaaaaag ccatagaaaa aatcaagcaa aacacacgtc actatgcgaa
    13081 gcgacaattg acatggttta agcatgacga agacatccaa tggtttcatc cagaggaaga
    13141 agaaaaaata attgattatg taactaaaaa cttgccacta aatgattgtt tatcataact
    13201 tttctctttt gccctacaat actttccagc ttgacataaa agcagattcg ttcatagaat
    13261 tcgattctgt tgaagagtta aaaaatattc ttgtgtcacc acttctttta tcttcgccta
    13321 atataatgat tattggaaga ggaagtaatt tgttgttttt agacgatttt aaaggtacaa
    13381 ttttacactc tggaataaaa ttcattaaaa aaattgacga aaacaatgat tttgtctttt
    13441 tagaagtagg ttcaggaatt atttgggacg atttcgtaca attttgtgtt gaaaacgatt
    13501 atcaaggaat tgaaaatctt tcgggcattc ctggtgaagt gggtgcagcc gccgtacaaa
    13561 atattggtgc ttatggtgtg gaaattcaag aaatgattga aaaagtaaat gttgtagaaa
    13621 ttcactctgc aaaaattcat gaatttgcca atgccgattg tcaatacggt tatcgaaaaa
    13681 gtatttttaa aaaagagttg aaaggaaaat tggtagttac ttctgtgatt tttcgcttga
    13741 ataaaaaatc aacttacaaa ttagagtacg aaggtctcga aaaagaagtc catgcaaaag
    13801 gagaaattaa tttgaaaaat attagacaag ccattttaga tattcgttca cgtaaattgc
    13861 ctgattacaa agaatttgga aacgctggca gtttttttgt caatccaatt atttcaaaag
    13921 aaaacttcca gaaattacag aaaagctatc ctaacattcc tcattatact atttctgaaa
    13981 aagaagaaaa aatacctgct gcctggctaa ttgaacaatg tggttggaaa ggaaaacaaa
    14041 ttggacaggt aaaaatacac gaacatcaac ctctaatttt aattaattgc ggaaatgctt
    14101 caggaaaaga aattgccaaa gtagctaacc aaattcaagc ttctgttaaa gataaatttc
    14161 aaatagagtt acaacctgag gtgaattata tttattcctg atattttcta ttcaaaaatt
    14221 cccattctat ttaaagcctg ctgatacttt ttggcatttt gcaagtgctc tttccagttt
    14281 gaagcaaaat tatgttcacc atttaaagtc tctttagcac acatataaag ataattgtga
    14341 ggcgtataat ttaaaaccgc atcaatactt ttttgtgagg gtaatcgaat aggtccagga
    14401 ggcaaacctt tgtatttgta agtattgtaa ggtgaatcta tttttaaata aattccgctg
    14461 attcttcgca aagcaaaatt tccagcagca aatttgagtg tagggtcagc ttgcaaaggc
    14521 attccctttt ttaaacgatt gatataaaca ccagcaacta tcggtcgttc ggattctaca
    14581 ttcgtttcct cttctacaat agaagccaac gtaattactt ccaatggagt aagtggtatg
    14641 gctgccgcct tttttctacg ttcttcattc cagaacctgt tatattcgtt atacattcga
    14701 gcaaataatt tttctggact tatgttccag aaaacttcat aggtatttgg aataaatatt
    14761 gaaattgaat tatatggatt taatccatac tcagcaagga aagttgaatc attcaacaat
    14821 tgaataatac ttgcactatc taccattaat tgcattgata atctgtgtgc taagtcttct
    14881 ttcgtgcgaa tattgttgat aattaataaa acaggagttt gccttccgct tcgtaatttt
    14941 ctgattaatt caaaattgtt cattccacgt tgtagttcat aacgcccaga ttttatatgc
    15001 gattcataat caagcagttt cgaggcgaca taaaaagaat atacatcaaa tacttttgtg
    15061 tttttgctca atgaatcaac cacttctttt agttgactat tttcgcggat ataaagaaaa
    15121 gttcttgttt ttttcggagg gaaaatattg gggaaaaata aaatatacag aaaatagact
    15181 aaaattattg aaataaaaat ggtagtccat ttgaatattt tctttaaagt tttcttcgtg
    15241 aaaaaggaat cactcataaa aataaaatta agagtacaaa aatactgttt tgttcagata
    15301 ttttaaaaat caatatcgtg aatcttttta aaagttagaa tcttatacag attttaaatc
    15361 tgctattaaa gaaattcctt aaaattgact atcctcgccg caagcagcga ggtattacg
//
LOCUS       DAOTKS010000002        15373 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_4291_length_15373_cov_7.427145, whole genome shotgun sequence.
ACCESSION   DAOTKS010000002 DAOTKS010000000
VERSION     DAOTKS010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 15373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15373
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4291_length_15373_cov_7.427145"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2727
                     /locus_tag="P5236_00070"
     CDS             <1..2727
                     /locus_tag="P5236_00070"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRU62794.1"
                     /translation="FLLSAQNTIVENFETGLPTSAPSSETSVSLSTGMWKIKGVYAKS
                     DNGSIRLAMNADGYAITPPIDKPVSVSFKHRGSGSGKVLTVQKSIDNGSSWTTIGTAT
                     VSSSSTYNTANISINEQGTKGVLLKFICGSSTIYIDDVTIVCSSIVDEPTIQASISAS
                     EITATSMKINFNKGNGGGRLLVYSKGEEVNWLPEDGTAYTNLPKVLTDNVIGVYSGNA
                     DFVTVTGLEAGETYYFYVFEYNGANDAINYLTQPVGKLTQRTVEAPSITTSVNAINFG
                     SVKIGISSKRSFTLLAKYLTPENGTITLTSSNDFLISINSSSGFGTSLTLNYSSATLL
                     STTIYVQFLPSELKSYSQILSISGGNAEVQVQLIGIGSNTDAKVYFISPSGNDNAEGT
                     FDAPWYNLQKAIDAALPGDTILVRGGTYYPTMMKDGTKTTIRINSSGTADKRITIKNY
                     PSEFSILNFKDQPKKQGIRGVQLNGDYWHIIGLHITQAGDNAIKLEGNHNIIEKCTFS
                     YNDDTGIQLGFGHNFSDTHPGISSNDGSYCAYNDIIDCDSYLNCDADNFGSDADGFAC
                     KMHNGKGNRFIRCRSWDNADDGWDLYETDYPVILIECWAWGSGRAENFDWVQASGSFQ
                     GNGNGIKMGGNGTGGSSKGKHEAWNCIAFNCNKTGSVKGFDQNSHGDGEKLVNCLAFG
                     CGYDFMFEKSGTNCEYYNNVCFGNIEIASGCTESNNAMLSTSTKAWKNNVIRGFSSSD
                     YVSLSEDDAKAPRGADGSMPKRFARLKSGSVLVDKGLSYNVPYTDEFAFLFQPIYGLA
                     RDLGPYELQEGEISGMQIIINPKAEFNLSILSNPCKSEAMIKFSSDFSGIASLKIVAL
                     DGHVIEECYNKNVETGVEYFVSISVNNLQKGMYLCVLSIGDQTKTAKLIVAK"
     gene            2826..4724
                     /locus_tag="P5236_00075"
     CDS             2826..4724
                     /locus_tag="P5236_00075"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRU62795.1"
                     /translation="MKKSFLLYYVLILISVFSTNAFGRKYYISPTGSDSNDGLTLQTA
                     WGTPNFAFQTIQSGDTLWVKGGTYIVNSTVKAKNGGTKTAPVCVFAIRGEKPVFDCSS
                     MRHYGSESSTYRGMDLRQPWWHVRGIKIYKAGYNGIIIAGENITVEGMTIEECGMDGI
                     SLGAGAVNTLILNCDSYRNCDVSSRGENGDGFAAKDGTGTVFRGCRAWENADDGWDVY
                     GGNQPILVDSCWSYGNGVNYWPDLISSSSYQGDGNGFKLGGGGGVDGNAPNVVVNSFA
                     FGNVGKGFDQNHNAWGVTCINCTGYNNNGMGNFAFQEVPSMGKHVMINNLSYAGTGQN
                     IATGSTETTNSWNLGLIFTDNMFESLNMEDIKVERDDDYRLTDARIMSLFKLKSNNPA
                     IDKGTVQTYIPLKPYYAIPFSGEKPDLGAKEFDQGNWTFPEPSETEPAEPETPENNDR
                     PVGEKTVIVDYPNQFTVEANGGTSVVWGPFSLASAECAVFDMQAVGSSNGTVIIQFST
                     DNQNWVQVGEQAKNGSTSWVTGKLIDLSDPDAPWGPTIYIRFYNNSTRDVNIKNLKIT
                     GTLYILNNINGIDSDYNRIISVRYFDFTGKEIKTIDKEGLYIKQTFFENGKIKSEKIF
                     KKNYFRGN"
     gene            4794..6623
                     /locus_tag="P5236_00080"
     CDS             4794..6623
                     /locus_tag="P5236_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU62796.1"
                     /translation="MKNLKKIFTILFLFYCNSIFYAQQLTHVIGYPEGFASLNGGVTG
                     GEGGPIVTVTNADELKAAVDGANTATIILVEGTITVSGELSARGNKTIVGLGTDARIT
                     GGGFGWNTKSNVIVQNITFEDAPDDLLKINNKCQNFWIDHCTFSDGANTNAGSHDGML
                     DITRGSQYVTLSYNKFFNHDKNILIGHSDSERGDTAIKVTLHHNWFDGTIQRNPRVRY
                     ATVHCYNNYHVNNQTYGIASACDANVLVENCYFKDTQIPLQVGVPGFSPAGYLVARGC
                     YFENCGTPQTNEKDMTGLFPYNYTLDDVMYVPCIAETYSGADKPYRTAENFECEATSP
                     NTPGEDNPNEDGCTWIKNDLFAPDSDVNGWFWFNDETTCGKYIPSMITLLPSSSGTSS
                     TLQTDKVGAGTDGTSGGSGAITGCIDLSKSATKGEYTGGSTIFQLPSCGEFKLKTSRT
                     GDNLFSVSVATSADGEFTQKYTYSGSKGIAEIDITQYVLSETPIWVKITNGATGSLNI
                     HGVSVKSYEKECPNQTKKIEKNDISKMWYSGNILTFKSEKTCPAEIVIFSIDGKKLDI
                     PFRGWIEAGIENKVKLNLNYPDNSVYIIVMTTNEGKNILKIKK"
     gene            6629..8251
                     /locus_tag="P5236_00085"
     CDS             6629..8251
                     /locus_tag="P5236_00085"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRU62797.1"
                     /translation="MKKFIIIIAELIFVLNLWGTNYYVSPNGNDANAGTLSSPKFNIS
                     ALVNTVNPGDTIFILPGTYNYSNIITLTKSGTASKRICIFGIGYSTAEERPLLNFSSQ
                     GPGDSNRAFFLKAGANYWHLKGFIIKEAGDNGIKIESNWNIIEFIDFDGCYDSGIQIG
                     LAKDSGSNDGSCAYNYILNCDSYNNCDSHKSGGNADGFACKLFCGPGNIFENCRAWNN
                     SDDGWDFYSNTFGVLLLNCRTWHNGDATTLRGNKSGNGNGFKMGGNSTYSRGTNIAVG
                     CIAFDMHYVGGGGKCFDQNDNQDPQVMLNCTTFDNVKGFSMASGSSGHTIKNCIGFNS
                     RDKIISDQDQNITQNNSWQFGPNQKDKQNGGIANYDDFIVDLSNNDIANQLAANPRNV
                     DGSIPDNGFCRLSPSSDCIDKGQVVSYTFMDKTYQIPYTGIAPDLGAYEYQPSTGIFH
                     TIQNNISTNLRITNTQITDIATVLFYLEKTEPISLSITDMSGKKVMNCIDNQIFPSGN
                     NYHIIYLGNLSKGMYILHLNSKNNRQSIKFIR"
     gene            8252..10585
                     /locus_tag="P5236_00090"
     CDS             8252..10585
                     /locus_tag="P5236_00090"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRU62798.1"
                     /translation="MKIRLFILFGFTFFCFNVIADVIVQAESGIFDGKIDTQHAGYTG
                     TGFVDFTNAVGSKLILEFSIAEEMPSAKVLVRWANGKNDDRAMSVIVNDELQIASVPF
                     GYSGGFTTWVETEITLNLRKGTNRIIFTSLTSNGGPNFDRITIVGATEGVKEYSLTVN
                     IVGKGSVVKTPDELFYPNGTTVQLEAIPDASKQATFLGWSGDITGTNPKTSILIDGNK
                     TVTATFKSSMHSAYYCAPQEKGGNDNNPGTINAPFFNLSKAISVMDAGDTVYMRGGTY
                     RYTATIFLTANGNENERYCIFNYQGEKPVLNFYDIFSNYSNISSTARGEARGFKITGD
                     YYYLKGLEICQAPDNGIKIEGSYNICEQLVLHHNGDGGIQIGLAKDKPDAADKVCCNL
                     VKNCDSYRNLDWGTSYENADGFACKLSPGAHNRFVGCRAWENADDGWDFYMTHYTIYV
                     DSCWTMGNGNPDLVATPDPDWEYGQKNTPPTSWSGDGNGFKLGGDDWAAKHVVRNCIS
                     FNGYETGACFSENNNADSLFIFNCVAWQGMKNFRVRAYPSDLRNNISFDPKVGGQGQL
                     YDLASGTTEKNNSWNSINGQYPLVPYKTADNKTFDQKSIYNQFVSTSKEDFLAPREPD
                     GSLPKNGFGKLVDNSFFIDKGTSIVRGINPVTLKSYDFSIPYFGAAPDLGAFEHLTPT
                     GVPQIVSSDPHLKAYPNPFYEATTFDIIAFESGNAQLNISDLTGKIVYATSISGLMQG
                     EKRTVRFNSQLISGVYLVNFINGNHSQILKLIKLDNK"
     gene            10582..13011
                     /locus_tag="P5236_00095"
     CDS             10582..13011
                     /locus_tag="P5236_00095"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRU62799.1"
                     /translation="MKKLFFFILTCTFFSTSFGKQYFIANNGNDTNLGTIESPFLGFD
                     AAYSAAQPGDTIWVRGGVYNVSTIWKIEKAGNVNAYYNLWGYTPDIRQGDSVILNISA
                     TNGIRITLDGKYWHIKNLVIQNADDNGMRIEGSYNIIENCVFRYNSDSGLTIQQSNGT
                     MPNDGTIAAFNLILNCDSYYNDKNVSNADGFACKLSPGAGNVFRGCRSWANGDDGFDF
                     YYSRFQIIVEDCWTFRNGLKGGNGNGFKSNGSASSSSTPRTQAPHVYIRCIAFDHKYT
                     AGSNNKGFDQNHNEGNVTMINCLSFDNEKNYAYQEDTQNGKNHVLRNCVGFEPYSETL
                     GRTLLPGEIINPGTKNEISYESSNTWFGNTVDAAYNSWNLAESLNNGWNPSRDDYESL
                     ELADAIAERQADGSLPDNSFGKLKPESQMKDAGISYTWELSLPLSSYPNNPTKPYYVK
                     STLVTTDDGMPDLGPFEIPSAINPFNQSPIVNITAPQAGNEFNLNEVILLQANATDID
                     GNIARVEFYVNNNLIGISYASPYTYDWTCSIAGEYTFMAKAYDNEGAFSNSEVINITV
                     KNANAEGQLGEKKLLLKLSGTSLTDEIQNKPWSTVNSVSISNNHIKLTYSSSKGGTII
                     SPFIDMSKYLPDSLNFTFASSGGGNRNLNVYVSSDGGATYSAPYLQAGAANNVPVPLK
                     MKLSDKVSGEGNFVLKLEAAGTVEVWNVEVSGYEKIESVISSPAKENVCILIVSSGKL
                     ILKMNLTELMPFTVDIYNMQGVKMFHQKDENSQKGINEMIININSYPSGMYIIKLNSG
                     KDNFTSKFIKP"
     gene            13030..14865
                     /locus_tag="P5236_00100"
     CDS             13030..14865
                     /locus_tag="P5236_00100"
                     /inference="COORDINATES: protein motif:HMM:NF013276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HRU62800.1"
                     /translation="MKNGLMILLFFAFSLNLYSVNEIVVAKDGSGDFTTIQDAINSIP
                     TSNNDWKTIVVKNGIYNEHVMIKSNYIALVGESRSGTRIEFSIPRAEWYAANGTNAGT
                     GVINIASNLHDIVIANMYIRNTYEGSEDYTEVIRSETGTTRLWFINLDVLCLWKDTFA
                     PWGKAGGMYYVSDCNFRGSIDAFCPRGYCYAINCRFTETKSSSPIWHEGVATEDQKLV
                     IQGGSVHSEYNKNVKLQNCQNYAKFYYLDVQLSDSITQLGKSATTYFYGVKGPSGLTW
                     FNNNLTLDERKLIDAEWTFDKKWNPERTMPRVLPFASLPQPYNGRYALSAGNLTLKWT
                     SARNAVSNKVYFGTANQLQLLGETTLDSMITPVLETNKTYCWHVNTVTANGEMEGQLW
                     FFSTGDNIDQSIDDNPTTPVEEDNTDYNSPRLNQYDTYLNCKTMPKEPAWIITSGSVN
                     WSQGIGYYWGSSSAVLTLKVNNCTKIEFTYLNGSTSRPIIITDGGTNNDITTYPVAAN
                     TESTVTFIPKTTGDDDIQVKTSSASGLTLTKIVLYGIKSEIGKIVSASDTFFTLTSHF
                     IQSDDLMDIFDISGEMVFEKVQHAKLLPGIYIVRSANRVKKILIK"
BASE COUNT         5207 a         2254 c         3006 g         4906 t
ORIGIN      
        1 tttttactga gtgctcaaaa tactattgta gaaaattttg aaacgggttt acctacaagt
       61 gcaccttcaa gtgaaacatc agtatcactt tcaaccggaa tgtggaaaat taaaggtgtt
      121 tatgcaaaat ctgataatgg aagcataaga ttggctatga atgcggatgg ttacgctatt
      181 acgccaccaa ttgataagcc agtatctgtt tcttttaaac atcgaggaag tggtagtggt
      241 aaggtgctca ctgtacaaaa atctattgat aatggaagtt cctggacaac tattggaact
      301 gctacggtaa gctcttcatc tacttataat actgctaata tctctattaa cgaacaaggg
      361 acaaagggag ttttacttaa atttatttgt ggttcaagta caatttatat cgatgatgta
      421 actattgttt gttcaagtat agtagatgaa ccaacaattc aagcatctat ttcagcttca
      481 gaaataactg ctacatcaat gaagattaac ttcaataaag ggaatggcgg aggccgctta
      541 ttggtgtatt ctaaaggaga agaagtaaac tggttaccag aagatggaac cgcttatact
      601 aatttaccta aagttttaac tgataatgtg ataggtgtat attctggtaa tgctgatttt
      661 gttaccgtaa cgggacttga agcaggtgaa acttattatt tttatgtatt tgaatataac
      721 ggagctaatg atgctattaa ttatcttact cagccagtag gaaaattaac tcaaagaact
      781 gtagaagcac caagtattac aacaagtgtt aatgctatta attttggaag tgtaaaaata
      841 ggtatttcat caaaacgcag ttttactctt ttagctaaat atttaactcc agaaaatgga
      901 acaatcacat tgacttcttc aaatgatttt ctgatatcaa taaattcttc aagtggattc
      961 ggtacttctc ttactttaaa ttattcttca gctactttat tatccactac tatttacgtt
     1021 cagtttttac cttctgaatt aaagtcttat tcacaaattc tttctatctc aggaggtaat
     1081 gctgaagtgc aggtacaact tatcggtatt ggttcaaata cggatgcaaa agtttatttt
     1141 atttcaccat caggaaatga taatgctgaa ggaacatttg atgctccgtg gtataatttg
     1201 caaaaagcta tcgacgcagc tttaccggga gatacaatat tagttcgcgg cggtacttat
     1261 tacccaacaa tgatgaaaga tggtacgaaa actactatac gtataaattc atcaggtaca
     1321 gctgacaaaa gaattactat aaaaaattat ccaagcgaat tttctatact taattttaaa
     1381 gatcaaccta aaaaacaagg tattcgtgga gttcaactaa atggagatta ttggcatatt
     1441 ataggattgc atattacgca agcaggagat aatgccataa aattagaagg gaatcataat
     1501 attatcgaaa aatgtacttt cagttataat gatgatacag gtattcagtt aggatttggt
     1561 cataattttt ctgatacaca tcctggtatt tcttcaaatg atggaagcta ttgtgcttat
     1621 aatgatatta ttgattgtga ctcatactta aattgtgatg cagataattt tggcagtgat
     1681 gccgatggat ttgcttgtaa aatgcataat ggcaaaggga atcgttttat tcgttgtcgt
     1741 tcatgggata atgcagatga tggatgggat ttatatgaaa cagattatcc agtcatatta
     1801 attgaatgtt gggcttgggg ttctggaaga gctgaaaatt ttgattgggt tcaagcctca
     1861 ggatctttcc agggaaacgg caacggaata aaaatgggag gaaatggtac aggtggttca
     1921 tcgaaaggaa aacatgaagc atggaactgc attgctttca attgcaataa aacaggttca
     1981 gttaaaggtt tcgaccagaa cagtcatgga gatggagaaa aattggtaaa ctgtcttgca
     2041 tttggttgtg gatatgattt tatgtttgaa aaatcaggaa ccaactgtga atattataat
     2101 aatgtttgct ttggtaatat tgaaattgct tcaggctgca cagaaagcaa caatgccatg
     2161 ttgtcaacct ctaccaaagc atggaaaaat aatgttattc gtggcttttc tagttctgat
     2221 tatgttagtt taagtgaaga tgatgccaaa gctccgagag gggcagatgg tagtatgcct
     2281 aaaagatttg cacgtttaaa aagtggtagt gtattagtag ataaaggatt atcttataat
     2341 gttccctata cagacgaatt tgcatttttg tttcaaccaa tttatggttt agcacgtgat
     2401 ttaggtcctt atgaacttca agaaggtgaa atttctggaa tgcaaatcat tataaatcca
     2461 aaagcagaat ttaatttgtc gattttatcg aatccatgta aatcggaagc aatgattaaa
     2521 tttagctctg atttttcagg aatagcttcc ttaaaaattg tcgctttaga tggtcacgtg
     2581 atagaagaat gttataataa aaatgttgaa actggagtag aatatttcgt ttctatttct
     2641 gtaaataatt tacagaaagg aatgtattta tgtgttttaa gtataggaga tcaaactaaa
     2701 acagcaaaac ttattgttgc aaaataagtt tattaacagt taacctttta ctagataatt
     2761 cttttaaata aatctaacaa attttcttaa aaagatatct aataaaatca aataagtatt
     2821 caaccatgaa aaagagtttt ttgttatatt atgtattgat cctgatttct gtattttcta
     2881 caaatgcttt tggacgaaaa tattatatat caccaaccgg tagcgatagc aatgatggac
     2941 tcacactaca aacagcatgg ggaactccga actttgcttt tcaaacaatc caaagcggtg
     3001 acaccttgtg ggtaaaagga ggaacttaca tagtaaattc tacagtaaaa gccaaaaatg
     3061 gtggaacaaa aactgctccg gtctgtgtat ttgctatacg aggtgaaaaa cctgtttttg
     3121 attgttcttc catgcgtcat tatggaagtg aaagttccac ttatcgaggt atggatttac
     3181 gtcagccgtg gtggcatgtt cgaggtataa agatttataa agctggatat aatgggatta
     3241 ttattgctgg tgaaaatatt acagtcgaag gaatgaccat tgaagaatgt ggaatggatg
     3301 gaatttcgtt aggtgccgga gctgtcaaca ccttgatttt aaattgtgat tcttacagaa
     3361 attgtgatgt ttcttcaaga ggagaaaatg gagatggatt tgctgcaaaa gatggaacgg
     3421 gaacggtatt cagaggttgt cgtgcatggg aaaatgccga tgatggctgg gatgtttatg
     3481 gaggtaatca gccaattttg gtagatagtt gttggtcgta tggaaatgga gtaaattatt
     3541 ggcctgattt aatatcaagc tcttcttatc aaggtgatgg aaatggattc aaacttggtg
     3601 gaggaggagg ggtagatgga aatgctccaa acgtagtagt aaatagtttt gctttcggaa
     3661 atgttggaaa aggttttgat caaaatcaca atgcttgggg agttacctgt attaattgca
     3721 cgggatataa taacaatgga atgggaaatt ttgcattcca ggaagttcca agtatgggta
     3781 aacatgtaat gatcaacaat ctttcatatg cgggtacagg acaaaatatt gctacgggta
     3841 gtactgagac caccaatagc tggaatttag gacttatttt taccgataat atgtttgaaa
     3901 gtctgaatat ggaagatata aaagtggaac gcgatgatga ttatcgttta acagatgctc
     3961 gcattatgtc gctttttaaa cttaaaagca ataatcctgc gattgataaa ggaacagtac
     4021 agacttatat tccattgaaa ccatattatg ctattccttt ttcgggtgaa aaaccagatt
     4081 taggtgcaaa agaatttgat caaggtaatt ggacttttcc tgaaccttca gaaacagaac
     4141 cagcagaacc cgaaactcca gaaaataatg atagacctgt tggcgaaaaa accgttattg
     4201 ttgattatcc aaatcagttt actgttgaag caaatggagg aacttcagtg gtttggggtc
     4261 cattttctct ggcctcagca gaatgtgctg tttttgatat gcaagcagta ggttcttcta
     4321 atggcactgt aataatacaa ttttcgacag acaatcagaa ttgggttcag gtaggagaac
     4381 aggctaaaaa tggttctaca agctgggtta cagggaaatt aattgatcta agcgatcctg
     4441 atgctccatg gggaccaacc atttatattc gtttttataa taattcaaca cgcgacgtca
     4501 atattaaaaa tttaaaaata acaggtacat tgtacatttt gaataacatt aatggaattg
     4561 attctgatta taatagaatt atctctgtcc gatattttga tttcactgga aaagaaataa
     4621 agacgataga taaagaagga ttatatatta aacaaacctt ttttgaaaat ggaaaaataa
     4681 aatccgaaaa aatcttcaaa aaaaattatt ttagaggtaa ttaattattt tttaaaatat
     4741 tataaattct tgtaatcata tttatataat aacttataaa aactaatact gaaatgaaaa
     4801 acttaaaaaa aatatttaca attttatttt tattttattg taattcaata ttttacgcac
     4861 aacagttaac tcacgttatt ggatatcctg aaggatttgc ttcacttaat gggggtgtaa
     4921 caggtggtga aggtggtcca attgttacag taactaatgc tgatgaatta aaagcagcag
     4981 tagatggtgc taacacagca acaattattc tggtagaagg aacaattact gtttcaggtg
     5041 aattatccgc tcgtggaaat aagactatag ttggtttagg aaccgatgca cgaattacag
     5101 gtggaggatt tggatggaat acgaaatcga acgttattgt tcaaaacatt acttttgaag
     5161 atgctcctga cgatttactc aaaataaaca ataaatgtca gaatttctgg atcgaccatt
     5221 gtacatttag cgatggagct aataccaatg cgggttcaca tgatggaatg ttggatatta
     5281 cgcgaggcag tcaatacgtt actctttcat acaataaatt ttttaatcac gataaaaata
     5341 tccttattgg gcacagcgat agtgaaagag gtgatactgc aataaaagta actcttcatc
     5401 ataattggtt tgatggtact attcaacgta atccgcgagt aagatatgcc actgttcatt
     5461 gctacaacaa ttatcatgtt aataatcaaa catatggaat agcttcagca tgtgatgcca
     5521 atgtactagt agaaaattgc tatttcaaag atacacaaat tcctttgcaa gttggtgtcc
     5581 cgggattttc acctgctggt tatttggtag caaggggctg ctattttgaa aattgtggaa
     5641 ctccccaaac gaatgaaaaa gacatgacag gtttgttccc gtataattat acactggatg
     5701 atgtaatgta tgttccctgt attgctgaaa cctattcagg tgctgataaa ccttatcgca
     5761 cagcagaaaa ttttgaatgc gaagctactt ctcctaatac tcctggagaa gataatccaa
     5821 acgaagacgg ttgcacatgg attaagaatg atttgtttgc tcctgattct gatgtaaatg
     5881 gttggttttg gtttaatgat gaaaccacat gcgggaaata tattccttcc atgatcactc
     5941 ttttaccgtc atcatcgggc acaagttcta ctttacaaac tgacaaagtt ggtgcaggaa
     6001 ccgatggaac atcaggaggt tcaggtgcaa ttacaggttg cattgatttg tcaaaatcag
     6061 caacaaaagg tgaatatacg ggtggctcta caatctttca acttccaagt tgcggtgaat
     6121 ttaagttaaa aacttcccgt acaggggata atttatttag cgtgtctgtt gctacttcag
     6181 ccgatggaga atttacacaa aaatatacct actcgggtag taaaggtatt gcagaaattg
     6241 atattactca atatgtcctt tctgaaacac ctatttgggt aaaaataacc aatggagcca
     6301 caggaagttt gaatattcat ggagtttctg ttaagtctta cgaaaaagag tgtccaaatc
     6361 agacaaaaaa aatagaaaaa aatgatattt caaaaatgtg gtattctgga aacattttga
     6421 catttaagtc tgaaaaaact tgtccagcag aaattgttat tttttctatc gatggaaaaa
     6481 aattagatat tccttttcga ggttggatag aagccggtat cgaaaacaag gtaaagttaa
     6541 atctaaatta tcctgataat agtgtttata tcattgtaat gacaaccaat gagggtaaaa
     6601 acatactgaa aattaagaaa taaagcttat gaaaaagttt attattatta tagcagaatt
     6661 gatttttgtt ttgaatctat gggggacaaa ttattatgtt tcgcccaacg gaaacgatgc
     6721 gaatgctgga acattaagtt ctcccaaatt taatatttcg gcattagtaa atacagtaaa
     6781 tcctggagat acaattttta tattacctgg tacttataat tattccaata ttattactct
     6841 cactaaatct ggaacagcca gtaaaagaat ctgtattttt ggtattggat atagtacagc
     6901 cgaagaacgt ccactgctta atttcagttc acaaggacct ggagattcca atcgtgcctt
     6961 tttcttaaaa gcgggagcaa attattggca tcttaaaggg tttattatta aggaagccgg
     7021 agataatgga attaaaatcg aaagcaattg gaacattatt gaatttatag attttgatgg
     7081 ttgttatgac agcggaattc aaatcggact tgctaaagat tcaggatcaa atgatggttc
     7141 atgtgcctac aattatatat tgaattgtga ctcatataat aattgtgatt cacataagag
     7201 tggaggcaat gcagatggtt ttgcctgtaa attgttttgc ggaccaggaa atatttttga
     7261 aaattgtcgc gcatggaata attcagatga tggctgggat ttttattcca atacttttgg
     7321 ggtattattg ttgaactgtc gtacatggca taatggtgat gcgactactc ttcgtggtaa
     7381 caaatcgggt aacggaaatg gtttcaaaat gggtggtaac agtacttatt cacgtggaac
     7441 caatatagct gttggttgta tagcttttga tatgcactat gttggtggag gtggaaaatg
     7501 ttttgaccaa aatgacaatc aggatcctca agtaatgctg aattgtacaa ctttcgataa
     7561 tgtaaaaggt ttctcaatgg cgagtggaag tagtggtcat actatcaaaa attgtatagg
     7621 atttaattca agagataaaa tcatcagtga tcaagatcaa aatattaccc aaaataatag
     7681 ctggcaattt ggacctaatc aaaaagacaa acaaaatgga ggcattgcca attatgatga
     7741 ttttatagtt gatttaagta ataatgatat agccaatcag cttgcagcta atcctcgcaa
     7801 tgttgatgga agtattcctg ataatggttt ttgcaggtta agtccttctt ctgattgtat
     7861 tgataaaggt caggtggtaa gctatacttt tatggataaa acttatcaaa ttccttatac
     7921 tggtatagct cccgatttag gtgcatatga atatcagcct tctacaggaa tttttcatac
     7981 tattcagaat aatatctcta ccaatttaag aattacaaat acccagatta ctgatatagc
     8041 tactgtttta ttttacttag agaaaactga accgatttct ctttctatta cagatatgtc
     8101 aggtaaaaaa gtaatgaatt gtattgacaa tcaaattttt ccatcaggga ataattatca
     8161 cattatttat ctgggaaatc tttcaaaagg aatgtatatc cttcatctta attctaaaaa
     8221 caaccgtcaa tctattaaat ttatacgata aatgaaaata cgtttattta ttttattcgg
     8281 atttactttt ttctgtttca atgtaattgc tgatgtaatt gttcaggctg aaagcggaat
     8341 atttgatggc aaaatagaca cccaacatgc tggctacaca ggaactgggt ttgtcgattt
     8401 taccaatgca gttggaagta aactgattct tgaattttct attgcagaag aaatgccttc
     8461 tgctaaagtg ctggtacgct gggcaaatgg gaaaaatgat gatagagcta tgagcgtaat
     8521 tgtaaatgat gagctacaaa ttgcaagtgt tccttttggt tattcaggtg gttttacaac
     8581 atgggttgaa actgaaataa ccttgaactt aagaaaagga accaacagaa taatttttac
     8641 ctcattaacc tctaatggag gtccaaattt tgacagaatt accattgttg gggcgactga
     8701 aggagtaaag gaatattcgt taactgttaa tattgttggc aaaggaagcg ttgttaaaac
     8761 tcctgatgag cttttttatc caaatggaac aactgtccag ttggaagcaa ttcctgatgc
     8821 tagtaaacaa gccacttttc tgggctggtc aggtgatatt acaggtacaa accctaaaac
     8881 ttctattcta attgatggca ataagacagt aacagccact tttaaatcat ctatgcatag
     8941 tgcttattat tgtgctccac aagaaaaagg tggaaatgat aataatccag gaactattaa
     9001 tgctccattt tttaacttat caaaagcaat tagtgtaatg gatgcaggcg atacggttta
     9061 tatgcgaggc ggaacatacc gttacactgc aactattttc ctgactgcaa atggaaatga
     9121 aaacgagaga tattgtattt ttaattatca gggtgaaaaa cctgttttaa atttctatga
     9181 tatttttagt aactattcaa atataagttc tactgcacga ggtgaagctc ggggttttaa
     9241 aattactgga gattattatt atttaaaagg tcttgaaatt tgtcaggcac ctgataatgg
     9301 aataaaaata gaagggagtt ataatatttg tgagcaactt gttttacacc acaatggaga
     9361 tggcggtata caaatcgggc ttgcaaagga taaaccagat gctgctgata aagtatgttg
     9421 taatttggtt aaaaattgcg attcatatcg taatcttgac tggggaacaa gttatgaaaa
     9481 tgccgatggc tttgcttgta aactttctcc cggtgctcat aatcgttttg ttggttgccg
     9541 tgcatgggaa aatgctgacg atggatggga tttttacatg actcattata ctatttatgt
     9601 tgattcttgc tggacaatgg ggaatggcaa cccagactta gttgcaactc ccgatcccga
     9661 ttgggaatat ggacagaaaa atactcctcc cacttcatgg agtggtgacg ggaatggttt
     9721 taaacttggc ggagatgatt gggcagcaaa gcatgtggtt agaaattgta tttcctttaa
     9781 tggctatgaa acaggcgctt gcttcagtga aaacaataat gcagatagct tattcatttt
     9841 caattgtgta gcatggcaag ggatgaaaaa ctttagagta agagcttatc caagtgatct
     9901 aagaaataat atcagttttg atccaaaagt aggtggtcaa ggtcaattat atgatttggc
     9961 aagtgggaca accgaaaaaa ataatagttg gaacagtatt aatggtcaat atcctttagt
    10021 cccgtataag acagccgata ataaaacatt tgatcaaaaa agtatttata atcagtttgt
    10081 gagtacctca aaagaagatt ttcttgctcc ccgtgagcca gatggaagtt taccaaaaaa
    10141 tggctttgga aaattagtgg ataattcatt ttttattgat aaagggacga gcattgttcg
    10201 aggaattaat cctgtcacat tgaaaagtta tgatttttca attccttatt ttggggcagc
    10261 acctgatttg ggagcttttg aacatctaac cccgaccggt gttccacaga ttgtttcttc
    10321 tgatcctcat ttaaaagctt atccaaatcc tttttatgaa gcaactactt ttgatattat
    10381 agcttttgaa agtggaaatg ctcaattaaa tatttctgat ttgacaggta aaattgttta
    10441 cgctacctct atttcaggac taatgcaagg agaaaaaagg acagttagat ttaattctca
    10501 actaattagc ggggtttacc tggttaattt tattaatggt aatcattctc agatattaaa
    10561 acttattaaa ctggataata aatgaaaaag ttatttttct ttatattaac ctgtaccttt
    10621 ttttccacaa gttttggaaa acagtatttt attgctaaca atggaaatga cactaattta
    10681 ggtacgattg agtcgccttt tcttggattt gatgctgcat attcggctgc acaacctggt
    10741 gacactattt gggttcgcgg gggagtgtat aatgtaagta ctatctggaa aatagaaaag
    10801 gcagggaatg taaatgctta ttataacctt tggggttata cacccgatat tcggcaagga
    10861 gattcggtaa tactaaatat aagtgctacc aatgggattc gtattacttt ggatggcaaa
    10921 tattggcata ttaaaaattt agttattcaa aatgctgatg ataatgggat gcggattgaa
    10981 ggaagttata atattattga aaattgtgtt tttcgttata attctgatag tggattaacc
    11041 attcaacaat ccaatggcac gatgccaaat gatggaacta ttgcagcttt taatttaata
    11101 ttgaattgtg attcatatta taacgacaaa aatgtgagta atgcagatgg ctttgcttgt
    11161 aaactttcgc caggtgcagg aaatgtattt cgtggctgtc gttcgtgggc aaatggcgat
    11221 gatggctttg atttttatta ttctcgtttt caaattattg tagaagattg ctggacattt
    11281 cgaaatggct tgaaaggtgg aaatggtaat ggctttaagt caaatggaag cgcaagtagc
    11341 tcttctactc cacgaacgca ggctcctcat gtatatattc gctgcatagc attcgatcat
    11401 aaatatacag ctggttcaaa taataaaggt tttgaccaga accataatga ggggaatgta
    11461 actatgatca attgcctttc ttttgacaac gaaaaaaatt atgcttatca agaagatacc
    11521 cagaatggga aaaatcatgt tttacgtaat tgtgtaggtt ttgaacctta tagcgaaaca
    11581 cttggacgta cccttttacc aggcgaaata attaatccag gaacaaaaaa tgaaattagt
    11641 tatgaaagct cgaatacgtg gtttggtaat acagtagatg cagcatacaa ttcgtggaat
    11701 ctggctgaat ctttaaataa tggttggaat ccttcgagag atgattatga aagtctcgaa
    11761 ttggctgatg ctattgcaga acgccaagcc gatggttcat tgccggataa tagttttgga
    11821 aaattaaaac ctgaaagtca gatgaaagat gctggcattt cctatacatg ggaattaagt
    11881 cttcctctca gctcttatcc caataatcca acaaaacctt attacgtaaa aagtacactt
    11941 gtaacaactg atgatggaat gccggattta ggtccttttg aaattccttc tgcaataaat
    12001 cctttcaatc aatcacccat tgtaaatatc accgctccac aggcaggaaa cgaatttaat
    12061 cttaacgagg taattctttt acaagctaat gcaacggata ttgatggaaa tatagcaaga
    12121 gtagagtttt atgtaaataa taatttaatt ggaatttcct atgcttctcc ttatacatat
    12181 gactggacat gctctattgc aggcgaatat actttcatgg caaaagctta tgataatgaa
    12241 ggtgcattca gcaattctga agttataaat attactgtga aaaatgcgaa tgcagaagga
    12301 caacttggag aaaaaaaatt attattgaaa ttaagtggca caagtttaac tgatgaaatc
    12361 caaaataagc cgtggtccac agtaaattct gtatccattt caaataatca cataaaactg
    12421 acttatagta gtagtaaggg tggaactatt atttctccat ttattgacat gtccaagtat
    12481 ctccctgatt cactgaattt tacatttgcc agttcaggtg gaggaaacag aaatctaaat
    12541 gtttatgttt cgtctgatgg aggcgctact tacagtgctc cttatttgca ggcaggagct
    12601 gctaataatg tgcctgttcc attaaaaatg aagttatcag ataaagtgtc tggtgaaggt
    12661 aattttgtgt taaaactgga agctgctggt acagttgaag tatggaatgt ggaagtttca
    12721 ggttatgaga aaattgaaag tgtaatttct tcaccagcaa aagaaaatgt ttgtatcctt
    12781 atagttagtt caggaaaact aattttgaaa atgaatttga cggaattaat gccattcact
    12841 gttgatattt acaatatgca aggagtcaaa atgttccatc agaaggatga aaattcccaa
    12901 aaaggaataa atgagatgat tatcaatatt aattcttatc catcaggaat gtacataata
    12961 aaattgaatt cagggaaaga taatttcact tcaaaattta taaaaccttg aaaatctaat
    13021 agatatttta tgaagaatgg attgatgatt ttattgtttt ttgctttttc attgaatctt
    13081 tattcagtaa atgaaatagt agtggcaaaa gatggttctg gagattttac caccatacag
    13141 gatgctatta actcaattcc tacatctaat aatgattgga aaaccattgt tgtgaaaaat
    13201 ggaatttata acgagcatgt gatgataaaa tcaaattata ttgcattggt tggtgaatcc
    13261 cgttccggta ctcgtattga attcagtatt ccacgtgcag aatggtacgc agcaaatgga
    13321 acgaatgccg gaaccggtgt tattaatatt gcttctaatc tccacgacat tgtgatagca
    13381 aacatgtata ttcgcaatac ctatgaaggt tcagaagatt acacggaagt tatccgcagt
    13441 gaaacgggaa ctacacgtct ttggtttatt aatctggatg tgctttgttt gtggaaagat
    13501 acctttgcac catgggggaa agctggagga atgtattatg tttccgattg caatttcaga
    13561 ggaagtatag atgctttttg tccacggggc tattgttatg ccatcaattg tcgttttact
    13621 gaaaccaaaa gcagtagtcc catttggcac gagggtgttg caacagaaga tcagaaatta
    13681 gtaattcaag gcggttcagt tcatagtgaa tataacaaaa atgtaaaact tcaaaattgt
    13741 cagaattatg ccaagtttta ttatttggat gttcagctct ccgatagcat tacgcaattg
    13801 gggaaaagtg ctacaactta tttttatggc gttaaaggac catccggatt aacttggttt
    13861 aataataatc tgacgctgga tgaaaggaaa ctcattgatg cagaatggac tttcgataag
    13921 aaatggaatc ccgaaagaac aatgcctcgc gttttgccat ttgcttcctt acctcagcct
    13981 tataatggcc gttatgcact ttctgctgga aatctgacgc tgaaatggac aagtgctcgc
    14041 aatgcagttt ctaataaggt gtattttggc actgcaaacc aacttcaact attaggcgaa
    14101 actacgttgg attcaatgat tacgccagta cttgaaacga acaaaactta ttgctggcat
    14161 gtaaatacag taactgctaa tggtgaaatg gaaggtcaat tgtggttctt ttcaacggga
    14221 gataatattg atcaatcaat tgatgataat cctacaacgc cagttgagga agacaataca
    14281 gattataatt ctcctcgtct taatcaatat gatacttatc tgaattgtaa aacaatgcca
    14341 aaagagcctg cgtggattat tacatcaggc agtgtaaact ggtcacaagg gataggttat
    14401 tattggggaa gttcctctgc tgtacttact ttgaaagtaa ataattgtac caaaatagaa
    14461 tttacttacc tcaatggcag tacttccaga ccaattatta ttactgatgg aggaacaaat
    14521 aatgatatta ctacttaccc tgtagcagct aatacagaat caacagtaac atttattcca
    14581 aaaacaaccg gcgatgacga tattcaagta aaaacaagca gcgccagtgg cttgactctg
    14641 actaaaattg ttttgtatgg gataaaaagt gaaataggaa aaatagtcag tgcttcggat
    14701 acttttttta ctcttacatc ccattttatt caatctgacg atttgatgga tatttttgat
    14761 atctctggag aaatggtttt tgaaaaagtt caacacgcaa aactgctgcc tggaatttat
    14821 atcgttcgtt cagctaatag agttaagaaa attttgataa aatgaaatta tggaattcaa
    14881 acatgaaaca aattatatta gaaaacttta tcatgctaaa aaacttaata actgttacta
    14941 aataaacaca aatccaacct cataccttat tgaaagcatt ctgcaaaatg attcaatcaa
    15001 ataaggtaat aaagttaaag atgtagggaa gtacagtgtt atgaaggtta aaatattaat
    15061 aaggtttttg agtaataaaa agataaaaat gaaattattt atgctaaaaa tttaaaaaat
    15121 gattatcttt aagaaaaatt tttttatcga gtaaaaacat gaaacaaatt atattagaaa
    15181 attttatcat gctaaaaact ttaataacaa actgtcacca aatgaataaa tccaacctta
    15241 ttaccttatt gaaagtttga tgaaaaataa ttcaatcaaa taaagtaata aggttaaagg
    15301 cgtggtgaaa atttaacgtt ataaaggtta tgtgttttaa ataaggtttt aaaaacaaat
    15361 caataaaaga tta
//
LOCUS       DAOTKS010000003        15076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_4413_length_15076_cov_4.736169, whole genome shotgun sequence.
ACCESSION   DAOTKS010000003 DAOTKS010000000
VERSION     DAOTKS010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 15076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15076
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4413_length_15076_cov_4.736169"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="P5236_00105"
     CDS             complement(<1..896)
                     /locus_tag="P5236_00105"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HRU62801.1"
                     /translation="MTCPSLLPPKKVAFFTLGCKLNFAETSHIGKQLMEAGYTKARSG
                     EEADICIVNTCTVTDAADRKCRQAIHRLHREYPHAIMIVTGCYAQLKPQEIAAIEGVN
                     LVLGSHEKFNILEYLQTFENQSNSQIFHSKQQELKTFHPSCSYEDRTRYFLKIQDGCD
                     YYCTYCTIPFARGRSRNGSISEIITLAEKAISEGAKEIVLTGVNVGDFGKSTGESFFN
                     LLKALDNLPYEIRFRISSVEPDLLSDEIIKWIAQSRHFMPHFHIPLQSGSNEILRIMR
                     RRYNTELFASKIDTIKKWMPNA"
     gene            1417..2709
                     /locus_tag="P5236_00110"
     CDS             1417..2709
                     /locus_tag="P5236_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRU62802.1"
                     /translation="MKTSNIPFLQGLLVFLFLMGFSCQGWASEKKQNYQQQAQNYLEY
                     KGKIIDSSNKKVLPFAHLRVEGTNISTVSNAEGEFLIKIPEELKDKKLIISYIGYKNA
                     EFPFYQLNTENIRIALEPLVIELPEINIISRDANELIKSVFEKRNVNYPNQKIFMTAF
                     YRESIKKNRSYVSLAEAIVDILKQPYTSYLSDEAQFYKSRKSTDYKKLDTLVFKLMGG
                     PYNSLYLDIMKYPEYLFMDDMMTNYEFTFDHVTYTDHQMIYIVDFKQQPYIKEPLYYG
                     KLYIDAETLALQSAIFNLNLDDKERAASLFIRKKPFNARVFATKASYRIDYIKRGEKW
                     YYNYSRIELNFKIDWKKRLFNSNYYSIIEMAITDWEPFTEEKKIKNKIKHTVILTDEA
                     SGFSDPSFWGEYNVIEPEQPIETAIKKIQKQLQKRTQN"
     gene            2963..4207
                     /gene="trpB"
                     /locus_tag="P5236_00115"
     CDS             2963..4207
                     /gene="trpB"
                     /locus_tag="P5236_00115"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRU62803.1"
                     /translation="MSENLNNSNHTVTGNLYQPNEKGYYGEFGGAYIPEILYATVEQL
                     KNAFLAIKDDENFKNEYYDLLKNYVGRPTPLYFARRLSETYGVNIYLKREDLNHTGAH
                     KINNALGQVLLAKRMGKTHMLAETGAGQHGVAAATACALMGMDCTVFMGKTDIERQYL
                     NVQKMRMLGAKVEPVTSGNMTLKDATNEAIRYWCSHPDSYYIIGSTVGPYPYPEMVGY
                     FQSVISEEIKWQLQEKIGRDYPDYLMACVGGGSNAMGTYYYYLNDNRVKIISAEAGGL
                     GIETGKTAATIHLGKIGIIHGAKTLILQNEDGQIDEPYSISAGLDYPGVGPAHAFFAK
                     IGRTKVYAINDDEAMEAAFELTRLEGIIPAIESAHALAILKKEKDNFKSDDVVVITVS
                     GRGDKDMDAYVKYYESMNKDKE"
     gene            4256..5671
                     /locus_tag="P5236_00120"
     CDS             4256..5671
                     /locus_tag="P5236_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HRU62804.1"
                     /translation="MKLYVNTKKLLADLDTPVGIYLKVRDLYTHSALLESSDYHGVEN
                     SYSYIGFCPIGGISVNHFLITEKYPDGTSIETEVKNKMTVAERFDSFLKSFEIIPSDQ
                     QTSPVVNGLFGYTSYEAVQYFEDVKLSARETVPGSMPGLNYLLFKYIIAINHFNNELT
                     IIENLLEGEESLIKSVEEILLNHNVATYPFQATGKEKCDISDEEYKQMVRRGKEECFK
                     GNVFQIVLSRRFYQSFQGDDFMLYRALRSINPSPYLFYFDLGDFRIFGSSPEAHLVVD
                     AKNRKAYIKPIAGTFRRTGDDKKDFELAEKLRNDDKENEEHVMLVDLARNDLSRNADD
                     VKVEAFREVQYYSHVLHLVSNVSGKLKPDTNVIKLFADTFPAGTLSGAPKIKAMQLID
                     EIEPHDRGPYGGCIGYLGFDKSFNQAIIIRSFISKNNTLYYQAGAGIVAKSNEENELQ
                     EVNNKLAALKQAIEVASSVSI"
     gene            5709..6281
                     /locus_tag="P5236_00125"
     CDS             5709..6281
                     /locus_tag="P5236_00125"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007752210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HRU62805.1"
                     /translation="MKILVFDNYDSFTYNLVHAVKKLGYDDVEVHRNDRIQLVDIDRF
                     DKILLSPGPGLPAESGILLEVVKRYASSKSILGVCLGEQAIAEAFGGTLLNLPEVYHG
                     VCSTIEIMKKDYLFNGLPQKIEAGRYHSWIVEKETLPSCLEVTAVDNQGMIMAIKHKI
                     YDVRGLQFHPESVLTPLGEKILKNWLQNTH"
     gene            6312..7310
                     /gene="trpD"
                     /locus_tag="P5236_00130"
     CDS             6312..7310
                     /gene="trpD"
                     /locus_tag="P5236_00130"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HRU62806.1"
                     /translation="MKEILNRLFDHQSLTREEAAEVMSNIGAGKYNESQMAAFLSVYL
                     MRSIELDELIGFRDALMTMAIPMDLSEFDPIDIVGTGGDGKNTFNISTCSCFTLAGAG
                     YKVAKHGNYGATSISGASNVIEYYGAKFTTDIDIAKRSLEESGFAYLHAPLCNPAMKN
                     VAPVRKNIGVRTFFNVLGPLISPVRPRYQCLGVYNLKMMRLYNYLYQKLGIQYSIVYT
                     FDGYDEISLTDKCKIINSEGEFIYTPEQLGFEKVNPAELWGGETVEHAAKIFMNILDN
                     QSTDGQRNVVIINSAFAIKTLCPEKAIEECKAEAIESLASGKAREAFMKFEEIYHL"
     gene            7320..8144
                     /gene="trpC"
                     /locus_tag="P5236_00135"
     CDS             7320..8144
                     /gene="trpC"
                     /locus_tag="P5236_00135"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HRU62807.1"
                     /translation="MEEKNEQKNSTIQKTDFLENILRNKREEVVLKKQSFKGKNWENF
                     PFFSRKCFSLKQNLLSLPIGIIAEFKRKSPSKGWLNQQADLFKVTTDYQQAGVAGISI
                     LTDTEFFGGSVEDVITVRDKISCPILRKDFVVDEFQIYEAKAIGADVVLLIAVALTIP
                     QVKNFARLAHQLGLEVLLEVHNKEELNYLNEYVDIVGVNNRNLHTFEVNIDTSLELAN
                     LIPSIFTKISESGISEIQTIRTLKQAGYRGFLIGERFIKEKNPADALRQFINQLIG"
     gene            8170..8853
                     /locus_tag="P5236_00140"
     CDS             8170..8853
                     /locus_tag="P5236_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HRU62808.1"
                     /translation="MKDNEKGREENNEVSQIANFKLIKVCGMKFPENIREVAALQPDF
                     MGFVFYKKSPRYVRHLDISVLDSLPSSIKKMGVFVNEKLDNILSTVYKYHLDGVQLHG
                     SELEDKCEKLKEAGLLVIKAFPVESSYNFIVTKPYESVCDYFLFDTKVPIYGGAGIKF
                     DWSLLNDYKGNTPFFLSGGISLEDVEDLKKIEHPKFIGVDLNSKFEIAPGKKNVELLR
                     QFFQLYRYS"
     gene            8874..9644
                     /gene="trpA"
                     /locus_tag="P5236_00145"
     CDS             8874..9644
                     /gene="trpA"
                     /locus_tag="P5236_00145"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRU62809.1"
                     /translation="MNRIQQLFQQKKENILSVYFTAGFPNLNDTVPVLESLQRKGIDL
                     VEIGIPFSDPLADGPVIQNSSQIALRNGMSLRKLFEQLTDVRSKIDIPLIMMGYLNPI
                     MQFGFENFCQECHRVGVDGMIIPDLPMDDYLTEYKDIAQKYGLEFVFLITPETSEKRI
                     RQIDEHTNGFIYMVSSPGVTGTRNSFDDHIDYFNRINSMHLKNPRLIGFGISNKATRE
                     TACQYSSGVIIGSAFIKALQETNDIDQAVDLLLERLNK"
     gene            10122..11453
                     /locus_tag="P5236_00150"
     CDS             10122..11453
                     /locus_tag="P5236_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA cytosine-C5-methyltransferase"
                     /protein_id="HRU62810.1"
                     /translation="MQLPTAFEERTRLFLGEEYPAFIKALNETPPTSIRVNDKMNYVP
                     SSQKVAWCESGYYLPERPSFTSDPLFHAGVYYVQEASSMFLQQAINQHFPEAKNILDV
                     CAAPGGKTTLLSQSFDNDTLIVSNEIIRSRVKILAENVIKWGNANVIVTNNEPADFSK
                     LSGFFDAVIVDAPCSGEGLFRKNPAAIQEWSVDNVQKCAARQRKIVQDVWDALKTGGI
                     LVYSTCTYNREENEENVFWICKELGAELLDIDVSRWKNITVSDGGYRFYPHKIRGEGF
                     FLSILRKTAKTPIRSYSLKKKETSKMQPISLPSISLKRPENWIFELNNQQLKAYPKNR
                     WKEIQLIQQTFSCVLSEIKLGEIKNEDFIPSAQLALSKELNLNTVEKIEVNYQTAIQF
                     LKKEPLIIDFFKKGFILLTYTGQPLGWLKNLGNRYNNLYPNEWRIRMKINE"
     gene            11555..12307
                     /locus_tag="P5236_00155"
     CDS             11555..12307
                     /locus_tag="P5236_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRU62811.1"
                     /translation="MKLLFLGTGTSTGIPQIGCQCKTCQSTDRKDKRLRASLLVNVQK
                     TNILIDAGPDLREQLLTYQISKLSGILLTHEHYDHIGGIDDLRPLGESHLYAEKRVLN
                     IIQKNMPYCFGDNLYPGVPMLQLHEITEKSKFYIDQVEIQAVRVMHAKLPILGFRIGN
                     IAYLTDVKTLDNKAIEQLQYLDLLVINALRQFPHISHLSLQEAIELSEKINAKETYFT
                     HISHDMGKHAEVEAHLPSHIHLAYDGLEIEIN"
     gene            12324..13331
                     /locus_tag="P5236_00160"
     CDS             12324..13331
                     /locus_tag="P5236_00160"
                     /inference="COORDINATES: protein motif:HMM:NF040069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6340 family protein"
                     /protein_id="HRU62812.1"
                     /translation="MKKCSYLIVIFINILLSSCSSLLFTTLDVLRPAQVAFPPEVNNL
                     LIVNNTPQDSIQPSKKSFTKDSLSIYCLSAFTEELINRGFFQNVLLELNSLNKAKNDT
                     SIYYLTPEVVTQLCSNYKSDAILALNKLKETDILTEYFVEDENSFLSTFEVFIETYWS
                     IHYPDKTDFTVLQFKDSMYWESENYSRQAALTQLPNKIDAQIDAALYAGRNCVKRFVP
                     YWEKVDRYFFNPRNKLMRQAMDSVYYKNWEAAIQLWDKAIQTTKNTKLQAQAANNIAI
                     AYEILGDLDKAIEYATLSLKSFLNVTTVDKETIIRISNYRNELIKRKKEIEQLEIQLG
                     E"
     gene            13393..14928
                     /locus_tag="P5236_00165"
     CDS             13393..14928
                     /locus_tag="P5236_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HRU62813.1"
                     /translation="MIHLSPFDEITLREKGLTISQIEEQLRFFETGFPFLEIVRPATV
                     DDGILKIQENEKKKLIALLDKYLYKSKIEIMKFVPASGAASRMFKDLYEFLESKEEIP
                     SNGVICQFFSEIDKFAFYDLLNETCVKNTGKNIQMLIHEKEYKIIIENLLNERGLNYG
                     SLPKGLLLFHTYPDEKRTPVQEHLVEGVLYTVNAQKEVNIHFTVSPQHKALFEKHISE
                     VKEKYEKKFKVHYSVSFSEQKPSTDTIAVDMNNQPIRENGKLVFRPGGHGALIENLND
                     LNSDIIFIKNIDNVVPDNLKRPTVTYKKILAGLLTNIQHQCFQYLNELENKNISEIKL
                     NEILQFTEKTLNIYHPNISAFDICSLRNYLITKLNRPLRVCGMVKNVGEPGGGPFFIV
                     NADGTISLQILESTQINTQNPADKEKMERSTHFNPVDLVCAVKNYKGEKFNLLNYVDK
                     KAGFISIKTKNGKKLKALELPGLWNGAMSDWNTIFVEVPLETFNPVKTVMDLLRSEHQ
                     TKE"
BASE COUNT         5233 a         2255 c         2677 g         4911 t
ORIGIN      
        1 aaagcattag gcatccattt tttaatggtg tctattttcg aagcaaagag ttcagtatta
       61 taacgtcttc tcatgattct aagaatttca ttacttcccg attgcagcgg aatatgaaaa
      121 tgtggcataa aatgacgact ttgagctatc cactttatta tctcatctga aagtaagtca
      181 ggctcaacag aagaaattct aaatcggatc tcgtaaggta gattgtccaa agctttcaat
      241 agattaaaaa aactttcacc tgtagatttg ccaaaatctc caacattcac acccgtcaga
      301 acaatctctt tagctccttc tgaaatagct ttttcagcaa gtgttataat ttctgaaata
      361 cttccatttc tacttctgcc acgtgcaaaa ggtatggtgc aataagtaca gtaataatca
      421 cagccatctt gaattttaag aaaataacgt gttcgatctt cgtatgaaca ggaaggatga
      481 aaagttttta actcttgctg ttttgaatga aatatttgtg aattactttg attttcaaag
      541 gtttgtaaat attccagaat attgaatttc tcgtggctcc ccaaaaccaa atttactcct
      601 tcgatggcag caatttcttg tggtttgagt tgagcataac aacctgtaac aatcataata
      661 gcatgtgggt attcacggtg aagccgatga attgcttgac gacattttcg atcagcagca
      721 tcggtaacgg tacaagtatt cacaatacaa atgtctgctt cttctcctga acgagctttg
      781 gtataaccag cttccattaa ttgtttgcca atgtgagaag tttcagcaaa attcaacttg
      841 caacccaatg tgaaaaaagc tactttttta gggggtaata aagaagggca tgtcattttt
      901 tataaaaaat tatgtacaaa attaatgaaa aatcttatga attttatgtt tttcactttc
      961 ttcttcaaga agacttctaa ttcaatacat taacaaaata cctactcatt tgttggtttt
     1021 tattcatacc aagttttaat cgaaattaaa attaaaagaa aaacactgag attttttgcc
     1081 cttctatttt acaggagacg ttttatgaaa tgtctctaat tgaaatcatt gattatcaac
     1141 taaatatata attcagaatt cagaactcac aaatctgcac ctcgcccctc gaaacccgta
     1201 tcttgtatct ctcaaacttt aaactctttt tatatgtttt ttcatttatg agaacaattt
     1261 actatcagct acctcactcg aaaaataatt ttaaaaaaac caactaatta tcataattat
     1321 ttgttatttt taaaaataaa agaattttct taaaaagttg aatttttcgt tttatctata
     1381 cttatttttt atgaaaaatt tctaatcata actcttatga aaacttctaa cattcctttc
     1441 cttcagggct tgttggtttt ccttttttta atgggtttct cttgtcaagg atgggcatca
     1501 gagaaaaaac aaaactatca acaacaagct cagaattacc ttgaatataa agggaaaatt
     1561 atagactcta gcaataaaaa agttttgccg tttgcccatc tcagagtaga agggactaat
     1621 atttctaccg tttcaaatgc agaaggagaa tttttgataa aaattccgga agaacttaaa
     1681 gacaaaaaat taatcattag ttatattggc tataaaaatg ctgaatttcc tttttatcaa
     1741 ttgaatacag aaaatatacg tattgctttg gaaccgttgg taatagaatt gcctgaaatt
     1801 aatattattt caagagatgc gaatgaatta attaaatcag tttttgaaaa acgcaatgta
     1861 aactatccga atcagaaaat tttcatgaca gctttttacc gtgaatctat caaaaaaaac
     1921 cgtagttatg tttcgttggc tgaagctatt gtggatattt taaaacaacc ttatacttcc
     1981 tatttgtctg atgaggcaca gttttacaaa agtagaaaaa gcacagatta caaaaaatta
     2041 gacacgcttg tttttaaatt aatgggggga ccttataatt ctttgtattt ggatataatg
     2101 aaatatccgg aatatctctt tatggatgat atgatgacca attatgaatt tacttttgat
     2161 catgttactt atactgatca tcaaatgatt tatatcgttg atttcaaaca acaaccttac
     2221 ataaaagagc ctctttatta tgggaaatta tatattgatg ccgaaacttt agctttacag
     2281 agtgcaattt ttaatttaaa ccttgacgat aaagaacgag cagcttcttt atttattaga
     2341 aaaaagcctt tcaatgccag agtctttgct actaaagctt cgtatcgaat tgattatatt
     2401 aaaagaggag aaaaatggta ttataattat agccgaattg aattgaattt taaaattgat
     2461 tggaaaaaaa gactatttaa ttcaaattat tattctatta ttgaaatggc tattactgat
     2521 tgggaacctt ttaccgaaga gaaaaaaatt aaaaataaaa ttaagcacac agtaattctc
     2581 accgatgaag cttcaggttt ttcagatcct tctttttggg gcgaatataa tgtaattgaa
     2641 ccagaacaac ctattgaaac agctattaag aaaattcaaa aacaactgca aaaaagaaca
     2701 caaaattaat ccaatttttt ttttgcacaa ttcaaaataa tttttcaact ttgcagcgat
     2761 aaacaaaaaa gtggaaatga aatcaaattc aacaatcaat aatttctggt ggtggcgctt
     2821 acgaaaattt aaatcgtgag aagggacacc tatggttgat agttgaaata aataaatatt
     2881 tatgaaaagg cttgtcggaa ctcacgacaa gccttttttt attaaaaaat ctcgaagaat
     2941 aaaaactaaa aataaaaaag atatgtcaga aaatttaaac aattcaaatc acacagttac
     3001 tggcaacctt tatcaaccca atgaaaaagg ttattatggt gaatttggtg gagcttatat
     3061 tcctgaaatt ttgtatgcca ccgtcgagca attaaaaaat gctttcttag ccatcaaaga
     3121 tgatgaaaac tttaaaaatg aatattacga tttgcttaaa aactatgttg gcagaccaac
     3181 acctctgtat tttgcccggc gactttcaga aacttatggt gtcaatattt atttaaagcg
     3241 ggaagacttg aatcataccg gagctcacaa aattaacaat gcgttaggtc aagtattgtt
     3301 agccaaacga atgggtaaaa cgcacatgct agctgaaacg ggagctggtc agcacggtgt
     3361 ggctgcagca accgcttgtg ctttgatggg aatggattgt accgttttca tgggcaaaac
     3421 agatattgaa cgtcagtatt tgaatgttca gaaaatgcgg atgctcggag ctaaagtgga
     3481 acccgttacc agcggaaaca tgacattaaa ggatgcgacc aatgaagcga ttcgttattg
     3541 gtgttcccat cctgattcgt attacatcat tggttcgacg gttggacctt atccttatcc
     3601 tgaaatggtg ggctattttc aatcggtgat tagtgaagaa atcaaatggc aactgcaaga
     3661 aaaaattgga cgggattacc ctgattattt aatggcttgt gttggtggcg gaagcaatgc
     3721 aatgggcact tattattatt atctaaatga taatcgggtg aaaattattt cagctgaagc
     3781 gggcggattg ggaattgaaa ccgggaaaac agcagcaact attcatctgg ggaaaatagg
     3841 catcattcat ggagcaaaaa cattgatttt gcaaaatgaa gatggccaaa ttgatgaacc
     3901 ttattctatt tctgctggtc tggattatcc gggagtgggt cctgctcatg ctttttttgc
     3961 caaaattgga aggacaaaag tttatgctat taacgacgat gaagcaatgg aagctgcttt
     4021 tgaactgact cgattggaag gaatcattcc cgccattgaa tcggctcatg cgctggctat
     4081 tctgaaaaaa gaaaaagata atttcaaatc ggatgacgtg gtggtaatta ctgtttccgg
     4141 gagaggtgac aaagatatgg atgcttatgt aaagtattat gaatcaatga ataaagacaa
     4201 agaataattt atttgaattt ctttttgaat tgaaatatta aaaattttaa ggattatgaa
     4261 actatatgta aacacaaaaa aattgctggc tgatctggat acgccagtag gaatttatct
     4321 caaagtaaga gatttatata cgcattcagc attgcttgaa agttctgact atcatggagt
     4381 tgaaaatagt tattcttata taggattttg tccaattggt ggaatatcgg ttaatcattt
     4441 tttgattaca gaaaaatatc cagatggaac ttctattgaa acagaggtaa aaaataaaat
     4501 gactgtcgct gaaaggtttg atagcttttt aaaatctttc gaaatcattc cttccgacca
     4561 acaaacttct cctgtagtaa atgggttgtt tggttatact tcttatgaag cagttcagta
     4621 ttttgaagat gtaaagttat ctgctcgtga aacagttcct ggaagtatgc caggtttgaa
     4681 ttatttattg ttcaaataca ttattgctat caatcatttt aacaatgaac tcaccattat
     4741 tgaaaatctt ctggaaggtg aagaatcgtt gataaaaagt gttgaagaaa ttttattaaa
     4801 ccacaatgta gcaacttatc catttcaggc aacgggaaag gaaaaatgtg atatttctga
     4861 cgaagaatac aagcaaatgg ttcgtcgagg caaggaagaa tgttttaaag gaaatgtttt
     4921 tcaaattgtg ctttcgcgtc gtttctatca gtcttttcaa ggagacgatt tcatgcttta
     4981 tcgggcttta cgttccatca atccttctcc ttatttattt tattttgatc tgggcgattt
     5041 tcgcattttt ggttcttcgc ctgaagctca tctggtagtt gatgcgaaaa acagaaaagc
     5101 ttatataaaa cctattgcag gcactttccg tcgtactgga gatgataaaa aggattttga
     5161 attggcagaa aaactacgca acgatgataa agaaaatgaa gagcatgtaa tgttggtaga
     5221 tttggcacgt aatgatttaa gtcgaaatgc agatgatgta aaagtagaag cttttcgtga
     5281 agtacaatat tattctcacg tattgcatct ggtatcgaat gtaagtggca aattaaaacc
     5341 cgataccaat gttatcaaac tttttgcgga tacttttcct gcaggtacac tttctggcgc
     5401 tccaaaaatt aaagccatgc agttgattga cgaaatcgaa ccacacgata gaggacctta
     5461 tggaggttgc attggctatc tgggctttga caaaagcttt aatcaagcga ttattattcg
     5521 ttcttttatt agtaaaaaca atacccttta ttatcaagca ggcgctggca ttgttgccaa
     5581 atcaaacgaa gaaaatgaat tgcaggaagt aaacaataaa ttggctgcac tcaaacaagc
     5641 tattgaagta gcttcgagtg tctcgattta aaaaaattga gttattattc tgaaaaattg
     5701 aaagttagat gaaaatactt gtgtttgata attatgattc ttttacctac aatctggtgc
     5761 atgccgtaaa aaaattagga tacgacgatg tggaagtgca tcgaaatgat cgcattcagt
     5821 tggtcgatat tgatcgtttt gataaaattc ttctttcgcc tggtccaggt ttaccagcag
     5881 aatcaggaat tttattggaa gttgttaaaa gatatgcttc cagcaaaagt attttaggtg
     5941 tttgcctggg agaacaggca attgcagaag cttttggagg aacgttgtta aatcttcctg
     6001 aagtatatca tggcgtttgt tctactattg aaattatgaa aaaggattat ttgttcaatg
     6061 gtttgcctca aaaaatagag gcaggaagat atcattcatg gattgttgaa aaagaaacat
     6121 tgccttcatg tttggaagtg actgctgtgg ataatcaagg aatgattatg gcgataaagc
     6181 acaaaattta tgatgtgcga ggtttacagt ttcatccgga atctgtttta acaccactgg
     6241 gagaaaaaat tctgaaaaat tggttacaaa atacacatta attttattga tttatcaaaa
     6301 attattctaa aatgaaagag atactgaaca gattatttga tcatcaatcg ttgacacgag
     6361 aagaagctgc cgaagtaatg agtaatatcg gagcagggaa atacaatgaa tcgcaaatgg
     6421 ctgcttttct ttccgtttat ctgatgcgaa gcattgagct ggacgaattg attggttttc
     6481 gagatgcttt aatgacaatg gcaattccga tggatttgtc tgaatttgat ccgattgata
     6541 ttgtcggaac aggtggagat ggaaaaaata cttttaatat ttctacttgt agttgcttta
     6601 cccttgcagg agcaggttat aaagtagcaa aacatggtaa ttatggagcc acatcaataa
     6661 gtggtgcttc caatgtaatt gagtattatg gagcaaaatt tactacggat attgacattg
     6721 ctaagcgttc gctggaagag tctggttttg cttatttgca tgctccattg tgcaatcctg
     6781 ccatgaaaaa tgtagctcct gttcgcaaaa atatcggtgt tcggactttt ttcaatgtac
     6841 ttggtccttt aattagtcct gttcgccctc gttatcaatg tttgggtgtg tataatttaa
     6901 aaatgatgcg tttgtacaat tatctttatc aaaaattggg tattcaatat tctattgttt
     6961 atacctttga tggttatgat gaaatttctt tgacagacaa atgcaaaatt attaacagtg
     7021 agggagaatt tatttacaca cctgagcaat tgggttttga aaaagtaaat cctgcagaat
     7081 tgtggggagg tgaaacagta gaacatgcag caaaaatctt tatgaacatt cttgacaatc
     7141 aatctactga tggacaacgc aatgtagtga ttattaactc agcttttgcc attaaaacgc
     7201 tttgtcctga aaaggccatt gaagaatgta aggcagaagc cattgaatca cttgcaagtg
     7261 gaaaagccag agaagccttt atgaagtttg aagaaattta tcatctttag ttttgtaaaa
     7321 tggaagaaaa aaacgagcaa aaaaactcaa ctattcaaaa aacagatttt cttgaaaata
     7381 ttttaagaaa taaaagagaa gaagttgtgt taaaaaagca atcttttaaa ggaaaaaatt
     7441 gggaaaattt tcctttcttt tctagaaaat gtttttcttt aaagcaaaat ttactttctt
     7501 tgccaatagg aattattgca gaattcaaac gaaaatctcc ttccaaaggt tggttaaatc
     7561 aacaggctga tttatttaaa gttacaacag attatcaaca ggctggtgta gcaggaattt
     7621 ccattttgac agataccgaa ttttttggcg gaagcgttga agatgtgata acagttcgtg
     7681 ataaaatttc ctgtcctatt ttaagaaaag attttgtggt agatgaattt caaatttatg
     7741 aagccaaagc cattggtgca gacgttgttc tgttgattgc tgtcgcttta actattcctc
     7801 aagtaaaaaa ttttgccaga ttggctcacc aattgggtct tgaagttttg ctggaggtgc
     7861 acaacaaaga agaattgaat tatttgaatg aatacgtaga tattgttggc gtaaataatc
     7921 ggaacttaca tacctttgaa gtaaatattg atacatctct tgaattggca aatttgattc
     7981 cttccatttt tacaaaaatt agtgaaagtg gtattagcga aattcaaact atcagaactt
     8041 taaaacaggc tggttatcga ggttttttga taggtgaacg ttttattaaa gaaaaaaatc
     8101 cagcagatgc tctacgacag tttatcaatc aactaattgg ataaaaatta aaattggaat
     8161 ttttttgaaa tgaaagataa tgaaaaaggt agagaagaaa acaatgaagt aagtcaaata
     8221 gcgaatttca aattaatcaa agtttgtgga atgaagtttc cagaaaatat tcgtgaagta
     8281 gctgctttac aacctgattt tatgggattt gttttttata agaaatctcc acgatatgta
     8341 agacatctgg atatttctgt gttggatagt ttaccttcgt cgataaaaaa aatgggtgtt
     8401 tttgtcaatg aaaagctgga taatattttg tcaacagttt acaagtatca tctggacggt
     8461 gttcaattgc atggcagcga actggaagat aaatgtgaaa aattaaaaga agctggtttg
     8521 ttggtgatta aagcttttcc tgtggaaagt tcgtacaact ttattgtgac aaaaccttac
     8581 gaaagtgttt gcgattattt cctctttgat accaaagttc ccatttatgg tggtgcaggg
     8641 ataaaattcg actggtcgtt attgaacgat tataaaggca atactccatt ttttctcagc
     8701 ggtggcattt cattggaaga tgtagaagat ttgaaaaaaa ttgaacatcc caagtttatt
     8761 ggtgtagatt tgaatagtaa atttgaaata gcgccgggca aaaaaaatgt cgaattgctc
     8821 aggcaatttt ttcaacttta tcgttatagt taaaaaatta attattagtt aaaatgaata
     8881 gaattcagca attgtttcag cagaaaaaag aaaatattct ttcggtttat tttaccgcag
     8941 gttttcctaa tttaaatgat acggttcctg ttttagaaag tttgcagaga aaaggaattg
     9001 atttggtaga aattggcatt ccattttccg atccattggc tgatggacct gttattcaga
     9061 atagtagcca aattgctttg cgaaatggga tgtcattacg aaagcttttt gaacagctta
     9121 cagatgttcg ctccaaaatt gatattccat tgattatgat gggttatttg aatcctatca
     9181 tgcaatttgg ttttgaaaat ttttgtcagg aatgccatag agttggagtg gacggcatga
     9241 ttattcctga tctcccaatg gatgattatt tgactgaata taaggatatt gctcagaaat
     9301 atggattgga atttgtcttt ttgattacac cagagacttc cgaaaaacgc attcgtcaga
     9361 ttgatgaaca caccaatggt tttatttaca tggtttcttc acctggcgtg acaggcactc
     9421 gaaattcatt cgatgaccat attgattatt tcaatcggat taattcaatg catttaaaaa
     9481 atccacgact cattggcttt ggcatttcca ataaagcaac gcgtgaaact gcctgtcagt
     9541 attcttcggg tgtcattatc ggtagtgctt tcatcaaagc tttgcaggaa acaaatgata
     9601 ttgatcaagc tgtcgatctt ttactggaaa gattgaataa ataaatgctg aatataactt
     9661 attaatcaac tgttggttag aaaaatttcg taataaattt acagggagtt aggaaattaa
     9721 ttcgtaaatt ttaatgtaat atagctttcc cttttaccaa atcatgatta tttaaatgat
     9781 tctttactat tacatggatt ctaatttgca aatacacaaa tttttttgaa aaagataaaa
     9841 ctattcgaaa tatgacattt tctaatctta attaaaaaaa taatgatttt tatttttatt
     9901 tcaaatgaca actttttaaa ttattattgt ttgctctatt ttaagaattt tttctttata
     9961 ttttcaaaaa taaataaatt tttaaaaaga aaaaaaatcc caatttcttt gtgtcaatat
    10021 agtttgacgg ttaaagtaca acaatctttt tacttttttt gtatttctat tcagacaaat
    10081 ctactaattt tgtgtcgcat tcaaatttca gaataatttt tatgcaactt ccaactgctt
    10141 ttgaagaaag gaccagattg tttttgggtg aagaatatcc agcattcata aaagcactca
    10201 acgaaacgcc acccaccagc attcgtgtaa acgataaaat gaattatgtc ccttcttctc
    10261 aaaaagtggc atggtgcgaa agtggttatt atttgcctga acgaccttca tttacaagtg
    10321 atcctctttt tcatgcggga gtttattatg tgcaggaagc cagttccatg tttttgcagc
    10381 aagctattaa tcagcatttc cctgaagcta aaaacattct ggatgtttgt gctgcgcccg
    10441 gtggaaaaac tactttactt tcccaatcct tcgataatga tacgttgatt gtgagcaatg
    10501 aaatcattcg ttccagagta aaaattcttg ctgaaaatgt catcaaatgg ggaaatgcca
    10561 atgtaattgt taccaacaat gaacctgctg atttcagcaa actttcaggt ttttttgatg
    10621 ctgtaatagt agatgctcct tgctctggcg aaggcttgtt tcgaaaaaat cctgctgcta
    10681 ttcaggaatg gtcagttgat aatgttcaaa aatgtgctgc caggcaaaga aaaattgttc
    10741 aggatgtatg ggatgcatta aaaactggag gtattttggt atatagcact tgtacttata
    10801 atcgagaaga aaatgaagaa aatgttttct ggatttgcaa ggaattagga gctgaattat
    10861 tagatattga tgtctctcgc tggaaaaata taacagtgag tgatggtggt tatcgttttt
    10921 atcctcacaa aattcgtggt gaaggatttt tcctttctat tttgagaaaa actgcaaaaa
    10981 cgcccataag gagttattca ttaaaaaaga aagaaacttc aaaaatgcaa ccaataagtt
    11041 taccctcaat ttctttaaaa cgtcctgaaa attggatctt tgaactaaat aatcagcaat
    11101 taaaagctta tccaaaaaac agatggaaag agattcaact gattcaacaa actttttctt
    11161 gtgttctatc tgaaataaag ctcggtgaaa taaaaaatga ggatttcatt ccttctgctc
    11221 aattggcttt gtctaaagaa ttaaatttga atacagtaga aaaaattgaa gtgaattacc
    11281 aaactgctat tcaattttta aagaaagaac ctttaataat tgattttttt aaaaaaggtt
    11341 ttattttgct tacttatacc gggcaaccgt tgggctggct aaaaaatctg ggtaatcgtt
    11401 acaataattt gtacccaaac gaatggcgta ttagaatgaa aatcaatgaa taaaataaaa
    11461 tatttttcac gtaagaaatt ttgctgaaaa aattattaaa aatattcact ttttaatcat
    11521 taaatttgac agaggaaaga acagagatag atatatgaaa ttactatttt taggaacggg
    11581 aacttcaacg ggaattcccc aaattggatg ccaatgtaaa acatgtcaat ccaccgatag
    11641 aaaagacaaa agattaagag cttcgctact tgtaaatgtg cagaaaacca atattttaat
    11701 tgatgcggga ccggatttaa gagaacaatt attaacttat caaattagca aactaagcgg
    11761 gatattgctc acacacgaac attacgacca tatcggggga attgatgatt tacgtccatt
    11821 gggagaatct catttgtatg ccgaaaaaag agttttaaat attattcaaa aaaatatgcc
    11881 ttattgtttt ggtgacaatc tttatccggg ggttccgatg ttgcaattgc atgagatcac
    11941 agaaaaatca aaattctata ttgatcaggt tgaaattcag gcagtgagag ttatgcatgc
    12001 caaattgcct attttaggat ttcggatagg taacattgct tatcttacag atgtaaaaac
    12061 tttagacaat aaagcaattg aacagcttca atatttagat ttgctcgtga tcaatgctct
    12121 cagacagttt cctcatatat ctcatttatc gttgcaagaa gccattgagc tgtctgaaaa
    12181 aatcaacgcc aaagaaactt attttacgca tatcagccac gacatgggaa aacatgctga
    12241 ggtggaagct catttaccat cccatattca tcttgcttat gatggattag aaattgaaat
    12301 aaactaactt aactttaata aaaatgaaaa agtgtagtta cctgattgtt attttcataa
    12361 atatcttgct aagctcttgt tccagcttgt tatttactac gctggacgta ttacgacctg
    12421 ctcaggtagc ttttccacca gaagtaaaca atttattaat tgtaaataat actccccaag
    12481 attctattca accatcaaaa aaatcattta ccaaagatag cctttcaatt tattgtttaa
    12541 gtgctttcac agaagaattg ataaatcgag gtttttttca gaatgttttg ctggaattaa
    12601 attcccttaa taaggccaaa aatgatacgt ctatttatta tttgacacca gaagtagtaa
    12661 cccaactttg ttccaattat aaaagtgatg caattcttgc tttaaacaaa ctgaaagaaa
    12721 cagatatttt aacagaatat tttgtagaag atgagaattc ttttctttcc acttttgaag
    12781 tttttataga aacctactgg agtattcatt atcctgataa gacagatttt acagtcttac
    12841 aatttaaaga ttcgatgtat tgggaatcag aaaattattc acgtcaagct gctttaactc
    12901 aacttccaaa taaaattgat gctcaaattg atgcagcttt atatgcagga agaaattgtg
    12961 taaaacgttt cgtaccttac tgggaaaaag tagatcggta ctttttcaat cctcgaaaca
    13021 aattgatgcg tcaggctatg gattctgttt attataagaa ttgggaagct gccattcaat
    13081 tatgggataa agcgattcaa acaaccaaaa acacaaaatt acaagctcaa gcagccaata
    13141 atatagctat agcttatgaa attcttggcg atttggataa agctattgaa tatgctactc
    13201 tttcgctaaa atcattttta aatgtaacga cagtagataa ggaaactatt atcagaattt
    13261 caaattatcg aaatgaattg atcaaaagaa aaaaggaaat cgaacaatta gaaattcaat
    13321 taggcgaata atactttgtt aaagaatata agaatatctt attttgtaat ataacattcc
    13381 aaaaaatagc gtatgattca cttgtcacct tttgatgaaa tcactttacg agaaaaagga
    13441 ttaaccatat cccagattga agaacagctc cgcttttttg aaacgggttt tcctttctta
    13501 gaaattgtac gacctgcaac agtagatgac ggaattttga aaatccaaga aaacgagaag
    13561 aaaaaattaa tagcattgtt ggataaatat ctgtacaaga gcaaaataga aataatgaaa
    13621 tttgttcccg catcgggtgc tgccagcaga atgttcaagg atttgtatga atttttggaa
    13681 agtaaagagg aaattccttc aaatggagtt atttgtcaat tcttttctga aattgataaa
    13741 tttgctttct atgacctatt aaatgaaact tgtgtaaaaa acacaggtaa aaatattcaa
    13801 atgctaatac atgaaaaaga atacaaaatc attattgaaa atttattgaa tgaaagggga
    13861 ttaaattatg gctctttgcc aaaagggttg ttgttatttc atacttatcc tgacgaaaaa
    13921 aggactccag ttcaagaaca tttagtagaa ggtgtacttt acacagttaa tgctcaaaaa
    13981 gaagtaaaca ttcactttac tgtttctcct caacacaaag ccttattcga aaaacatatt
    14041 agtgaagtaa aggaaaaata tgagaaaaaa tttaaagttc attattccgt aagtttttcc
    14101 gaacaaaaac ccagtaccga taccattgct gtggacatga acaatcaacc gattcgtgaa
    14161 aatgggaaac ttgtttttag accgggtgga catggggctt tgattgaaaa tttgaatgat
    14221 ctcaatagtg atattatttt tataaaaaac attgacaatg tagtacctga taatttgaaa
    14281 agaccaactg taacttacaa aaaaattctg gctggacttc tgaccaatat tcaacatcaa
    14341 tgtttccaat atttaaacga gctcgaaaat aaaaacattt cagaaataaa attaaatgaa
    14401 attttacaat ttaccgaaaa gaccttaaat atttatcatc ctaatatttc tgcattcgat
    14461 atttgttctc ttagaaatta tttaattacc aaactgaatc gccctttacg agtttgcggc
    14521 atggtaaaaa atgtaggaga gccaggaggt ggaccttttt ttatcgtcaa tgcggatggc
    14581 accatttctc ttcaaatttt agaaagcaca caaattaata cacaaaatcc tgctgataaa
    14641 gaaaaaatgg aacgttctac ccattttaat cctgtggatt tggtatgtgc tgtaaaaaac
    14701 tataaaggag aaaaatttaa tttgttaaac tacgtagata aaaaagcagg gtttatttca
    14761 attaaaacaa aaaatgggaa aaaattaaag gcactggaac ttccagggtt atggaatgga
    14821 gcaatgagtg actggaatac aatttttgta gaagtgcctt tagaaacttt caatcctgta
    14881 aaaacagtga tggacttgct tcgttctgag catcaaacaa aagaataaaa aatgatactg
    14941 ttagatttaa gaaaacaaaa aatcagtgat gagttttaat aattatctct catcactgat
    15001 ttctgaaact ttattttttt atatgattat ccgcaagaag acctattata aattcttgat
    15061 ctgaaatcgg agttgt
//
LOCUS       DAOTKS010000004        12621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_5423_length_12621_cov_4.122235, whole genome shotgun sequence.
ACCESSION   DAOTKS010000004 DAOTKS010000000
VERSION     DAOTKS010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12621
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5423_length_12621_cov_4.122235"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="P5236_00170"
     CDS             complement(<1..326)
                     /locus_tag="P5236_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004383265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRU62814.1"
                     /translation="MKVYQPNEIKNISLLGSAGSGKTTFAEAILYEAGIIKRKGSIAN
                     QNTVSDYYPVEKEYGYSVFPTVIQTEWQHNKLNFIDCPGADDFIGGVVTSLNVTDTAL
                     ILVNSQ"
     gene            complement(533..1408)
                     /gene="murI"
                     /locus_tag="P5236_00175"
     CDS             complement(533..1408)
                     /gene="murI"
                     /locus_tag="P5236_00175"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HRU62815.1"
                     /translation="MQSNIKDCLKDLSHNFESKPGPIGIFDSGYGGLTILEKIRQCLP
                     QYDYVYLGDNARTPYGTRSFEIVYKYTLECVVQLFKMGCHLVILACNTASAKALRTIQ
                     QNDLPILDSHRRVLGIIRPTVEAIGSITNNFHVGLLATSGTVQSNSYGLEIQKLYDNI
                     VLTSEACPMWVPLIENSEYNSPGADYFINKNLNHLLQIDPEIDTIILGCTHYPILENK
                     IKSLLPNKITVVSQGKIVAESLKNYLQRHPEIETLCTKKGTFQYYTTESVEKFSMMAS
                     IFLKENIQAQHIEIG"
     gene            complement(1437..2405)
                     /locus_tag="P5236_00180"
     CDS             complement(1437..2405)
                     /locus_tag="P5236_00180"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRU62816.1"
                     /translation="MAKKKYIFNPETLSYEPVEYTFGYKIRRFLVHTFTGIMIGTLFF
                     FVLALTLQSPEEKQLNDEKNKIEAQYQILERQFNEAQVVLNDLQERDNNLYRVVLQAE
                     PIPFSVRHAFAANADYYHQLLNMTNSEIVVNTTKELNVLKKQLYLQSKSYDELILLAK
                     NKETMLQHLPAIQPVLNKDLARMASGFGWRIDPIYHTQRFHEGMDFVAPTGTEIFATG
                     DGTISRAGWEQGYGNCVKISHGFGYETLYAHMSKIKVRIGQRVKRGDVIGLVGNTGKS
                     TAPHLHYEVHYKGQIMNPQNYYFLDLSPEEYDRMVQMSNNSGQLLD"
     gene            2608..5226
                     /gene="alaS"
                     /locus_tag="P5236_00185"
     CDS             2608..5226
                     /gene="alaS"
                     /locus_tag="P5236_00185"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRU62817.1"
                     /translation="MTSQEIRQSFLDFFAEKDHKIVPSAPMVIKDDPTLMFTNAGMNQ
                     FKNIILGNDPIKYPRVANVQKCLRVSGKHNDLEEVGHDTYHHTMFEMLGNWSFGDYFK
                     KEAIEWVWEYLVKVLKIDPDRLYVTVFEGSPADGLERDEEAAAIWSKFLSPERIINGS
                     KKDNFWEMGEVGPCGPCSEIHIDIRKDEERLKTDALTLINKNHPQVIEMWNIVFMQYN
                     RKANGSLMELPAKVIDTGMGFERLCMVVQGKTSNYDTDLFQPIIKEIGQICKAEYGHD
                     EKIDVAMRVVADHIRAIAFSIADGQLPSNTKAGYVIRRILRRALRYGYTFLHQRSPFL
                     FKLVDVLCQTMGSAYPELIDQKSLIEKVIKEEEESFLRTLETGIQLLEKMIEKAINEK
                     KKEISGKDAFMLYDTFGFPIDLTELILRENNLSVNMNEFNAEMEKQKERARNAANVET
                     GDWIILREGESQFVGYDVTSCNTEILRYRKVKQKNNEFYQLILSKTPFYAEMGGQVGD
                     SGWLLCGEDKIEILDTKKENNLSVHIVKRLPANLTDRFIASVDIEKRLATSRNHSATH
                     LLHEALRQVLGTHVEQKGSYVGPEGLRFDFSHYQKLTKKEIRAVEAIANRKIRENIPL
                     NEQREIPIQKAREMGAMALFGEKYDETVRLVQFGTSLELCGGTHVKATGEIGMVRIIS
                     ETSIAAGIRRIEAITGEAVEKLIDTQQDLLNEAKELLNNSPDLITAIRKLLEENRELK
                     KLVNEYNEEKLLQLRDKLLREAEIVEGITVVCYEGNLTSDQAKALAFQIKNLQNSNLF
                     FIAGNVHEGKPSLTVLITEDLVKKGFNAIDIVRKAAKEIQGSGGGQPFFAVAGGKNPQ
                     GIHKAVALTRELIKKE"
     gene            5486..6319
                     /locus_tag="P5236_00190"
     CDS             5486..6319
                     /locus_tag="P5236_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HRU62818.1"
                     /translation="MKRVAIQGISGAFHEIAAREYFKNEEIEIVPCNTFKDVFSAIEN
                     DKTLLGIIAIENTIAGSLLQNHNLLRESECLIVGEQKLHIEHHLVALAGQQLEDLEEV
                     HSHPIALMQCEEFLESHKHLKAVESEDTALSAKEIVDYQIYKRAAICSLYAAQKYGLE
                     VIERNIETNKKNSTRFLIVAEPTLALEMTRNDKINKASLVFALPHLEGSLSQVLSILS
                     FYNMNLTKIQSLPIIGREWEYQFYIDLIFNDYSRYKQAIDAILPLIDEFQILGEYHVD
                     I"
     gene            6377..7528
                     /locus_tag="P5236_00195"
     CDS             6377..7528
                     /locus_tag="P5236_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRU62819.1"
                     /translation="MNIQPAKRLNNVSEYYFSAKLREIDQMNAAGKKVINLGIGSPDL
                     MPSPQTIEALCQEAHKPNVHGYQSYNGIPELRNGFAEWYKKWFHVDLNPDNEIQPLIG
                     SKEGILHITLAFVNPGDSVLVPNPGYPTYTSVSKLCEANVIPYDLNENNNWQPDFEKL
                     EKMDLTNVKLMWVNYPNMPTGANASVELFEKLVDFSKRHNIVICNDNPYSFILNDKKL
                     SILSIPGAKDICIEMNSMSKSHNMAGWRIAMLASNAQFVQWVLKVKSNVDSGMFRPMM
                     IAAATALKNSEEWHNEMNKIYARRQQLAFKIMDQLGCTYDPKQVGLFVWGKIPAHYAD
                     AGELADEILYKKNVFITPGFIFGDKGKRYIRISLCCAEPVLEEVLKRLS"
     assembly_gap    7664..7763
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7764..8840
                     /locus_tag="P5236_00200"
     CDS             <7764..8840
                     /locus_tag="P5236_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HRU62820.1"
                     /translation="GIEAKRPIVIAGPCSAETEEQVIETARELASFGIKIFRAGVWKP
                     RTKPGGFEGVGVKGLPWLKRVKTEFGMYTTVEVANAKHVHEALANDVDILWIGARTSA
                     NPFAVQEIADALEGIDIPVLIKNPINPDLDLWIGAIERIYNAGIRRIAAVHRGFSSYD
                     KKIYRNLPQWHIPMELHRQIPNLPLFCDPSHIGGKRELIGPLSQQAMDLNFDGLMIES
                     HCNPDEAWSDAAQQVTPKILDFILNSLVIRETTQTTENLTALRRQIDELDNDLLQLLA
                     KRMRVSEEIGVYKKEHNMPIFQPERYDEMLKDRVKQAIQMGMDGDFMKNVLIAIHEES
                     IRHQMVIMEKSDATLNNSNSSTIQ"
     gene            complement(8890..9933)
                     /locus_tag="P5236_00205"
     CDS             complement(8890..9933)
                     /locus_tag="P5236_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU62821.1"
                     /translation="MKIKILSLAILLLVAAQAPAQRAFTIEAQNYDISDNLDLRAVVS
                     IFAESKNLKDFEMRLNDERYQISNLDLNNDGWIDYLRVVETSERDLHLVVIQAVLDRD
                     TYQDVATIVVEKRNNPQIYVRIIGNPYLYGVNYIIEPVFVREPVIFTFFWSSHYRPWR
                     SPYYWEYYPKFYHPRPPVKVNIYMGHIHRYIPRDCKFYYHTTVNIYHNDVYVKLHQTV
                     RRNDYERKHPEKSFNNRNKGIANKAEFNSARRTSSEANRRKVNQTNENYRKTSSRNLQ
                     SSSSSGNVERRNQNSSGGKEINNPPQKERKVSGTNRRSAESSASPSQKSSTRSSRGNA
                     AEHSNNQKHSERR"
     gene            10365..11315
                     /gene="ftsY"
                     /locus_tag="P5236_00210"
     CDS             10365..11315
                     /gene="ftsY"
                     /locus_tag="P5236_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRU62822.1"
                     /translation="MSILNFFNKDKKETLEKGLEKTRESVFSKISRAIAGKSKVDDEV
                     LDELEEVLITSDVGVETTLRIIDRIEKRVARDKYVNTNELNTILKEEIAALLAENNIE
                     SVADFSANLPAKPYVIMVVGVNGVGKTTTIAKLAYQYIKEGKKVFLGAADTFRAAAIE
                     QLSIWGDRVGAHVVKQKMGADPASVTYDTIASAMANDADVVIIDTAGRLHNKINLMNE
                     LTKIRNVMQKVMPGAPHEVLLVLDGSTGQNAFEQAKQFTKATDVTALAITKLDGTAKG
                     GVVLGISDQFKIPVKYIGLGEGLEDLQVFDKMAFVESLFE"
     gene            11372..>12621
                     /gene="rimO"
                     /locus_tag="P5236_00215"
     CDS             11372..>12621
                     /gene="rimO"
                     /locus_tag="P5236_00215"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007485681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRU62823.1"
                     /translation="MKKKDTIDIITLGCSKNLVDSEFLMRQFEALGYRVRHDAEVPDG
                     NIVIVNTCGFIADAKEESIYTILQFAKQRKENKIDKLFVMGCLSQRYLKELQEEIPEV
                     DKFYGKFDYVGILKELGENYQSHLRLERTLTTPAHYAYVKIAEGCNRTCSYCAIPLMT
                     GKYQSRPMEDIEKEVKILVSKGVKELQLIAQDLSDYGEDLYKTSKLAELIERLSDIQG
                     VEWIRLHYAYPVRFPFDVLKVMRERDNVCKYLDISFQHISDKMLKLMRRNITKKQTYE
                     LIDRIREEVPGIHLRTTLMVGHPGETEEDIQELKEFIRKEKFERLGAFTYSHEEGTFA
                     YQNYEDEISEKEKLQRLDKIMELQQQISFELNQSKIGKVFKVIIDRVEGEYYIGRTEF
                     DSPEVDPEVLIPISNKNIKIGEFS"
BASE COUNT         4276 a         1798 c         2332 g         4115 t
ORIGIN      
        1 tattgagaat tgacaagaat caaagctgta tcagtgacat tcaatgaagt taccacacct
       61 ccaataaaat catctgcacc tggacaatca ataaaattca atttattatg ttgccattct
      121 gtttgaataa ccgttggaaa aactgaataa ccatattctt tttcaacagg ataataatct
      181 gaaactgtat tttggttggc aatggaaccc ttccgtttaa ttatccctgc ttcatagaga
      241 atagcttcag caaaagttgt ttttccagaa cctgcacttc ccaataaaga gatgttttta
      301 atttcatttg gttgataaac tttcatatct cattcatttt tagaatatta taataattac
      361 gtagtgtttt ttataatgca aaaattggat ggcaagttat aaaaaactct cctattataa
      421 aaggagattt ttttatgttt ttaaaataat gttttaaaac ataaattgat accctttttt
      481 gggggttatt aatattggtg ttttttccaa tgaaacgtaa ttttcaatag aattaaccaa
      541 tttctatatg ttgagcttga atattttctt ttaaaaaaat agaagccatc atagaaaact
      601 tttctactga ttcagtagta taatattgaa aagttccttt ttttgtacag agcgtttcta
      661 tttctggatg acgctggaga taatttttta aactttcagc aacaattttt ccttgcgaaa
      721 caacagtaat tttattaggt agtaaagatt ttattttgtt ttccagaata ggataatgcg
      781 tgcagcctaa tataattgta tcaatttcag gatcaatttg caagagatga ttcaaatttt
      841 tattgataaa ataatcagct cctggagaat tatattcaga attttcgata agtggaaccc
      901 acataggaca tgcttcagaa gttaagacaa tgttatcata taatttttgt atttcaagcc
      961 cataggagtt tgattgaaca gttccagagg tggcaagtag acccacgtga aaattatttg
     1021 taatagaacc aatggcttct actgttggac gaataatacc taaaacacgc cgatgagagt
     1081 ccaaaatagg aaggtcgttt tgttgaatgg ttcttaaagc ttttgctgaa gctgtattac
     1141 aagctaaaat taccaaatga caacccattt taaaaagctg aacaacacat tccaatgtat
     1201 atttatacac tatttcaaat gatcgtgtac cataaggtgt acgtgcgttg tcgcctaaat
     1261 aaacataatc atattgaggt aaacactgac gaattttctc taaaatagtc aatcctccgt
     1321 atcctgaatc aaaaattcca atgggacctg gtttactttc aaaattatgt gataaatctt
     1381 ttagacaatc ttttatgttg ctttgcatga ttgaaatgaa ttttaattca gtagttttaa
     1441 tctaacaatt gacctgaatt gttactcatt tgaaccatac gatcatattc ttcaggcgat
     1501 aaatcgagga aataatagtt ttgcgggttc atgatttgtc ctttatagtg aacttcgtaa
     1561 tgtaaatgtg gggcagttga tttacctgtg ttccctacca acccaatgac atcaccacgt
     1621 ttaacacgtt gtccaattcg tacttttatc ttactcatat gggcatataa agtttcataa
     1681 ccgaaaccat gactaatttt cacacaattt ccataacctt gttcccaacc agctctacta
     1741 atagttccgt ctcctgttgc aaaaatctct gtccctgttg gtgctacaaa atccattcct
     1801 tcatgaaatc gttgcgtatg gtagatagga tcaattctcc acccaaatcc agatgccata
     1861 cgtgcgagat ctttgttcaa tacgggctga atagcaggaa gatgctgaag cattgtttct
     1921 ttattttttg ctaacaaaat taattcatca taagattttg attggagata aagttgtttt
     1981 tttaagacat tcaattcttt tgtagtattt acaacgatct ccgaattagt catgtttaaa
     2041 agttgatggt aataatcagc attagctgca aaggcatggc ggacagaaaa aggaataggt
     2101 tctgcctgaa gaacaactcg atataaatta ttatctcttt cttgtaaatc attaagcaca
     2161 acttgtgctt cattaaactg acgttccaga atttgatatt gtgcttctat tttatttttt
     2221 tcatcattca attgtttttc ttcaggagat tgtaatgtta aagcaagtac aaagaagaat
     2281 aaagtaccta tcataattcc tgtgaaagta tgaactaaaa atcttctgat tttatagcca
     2341 aaagtatatt caacaggctc ataacttaat gtttcaggat taaaaatata ttttttcttt
     2401 gccatagcaa tatcctttca taattaaatt agtgaaatat tagcgggaga aaatataaat
     2461 tttccattaa aaataaattt taaacgagct tatgatacaa aaataataaa tttaaactaa
     2521 ttttgcagac tccatttaag aatattaact ttaaaaataa aattgtattt ttgaataagt
     2581 tataattgaa ttacaacgat atatctaatg acttcacaag aaattcgtca atcttttttg
     2641 gatttttttg ctgagaaaga tcataaaatt gtcccatctg ctcctatggt aataaaagat
     2701 gatcctactt taatgtttac caatgctggg atgaaccaat ttaaaaatat tattttaggt
     2761 aatgatccta taaaatatcc acgtgtagcc aatgtacaaa aatgtttacg tgtgagtggt
     2821 aagcacaatg atttggaaga agtagggcat gatacttatc atcataccat gtttgaaatg
     2881 cttgggaact ggtcttttgg agattatttt aagaaagaag ctattgaatg ggtatgggaa
     2941 tatttggtaa aagtacttaa aattgatcct gaccgattgt atgttactgt ttttgaaggt
     3001 tcaccagcag atggtttgga acgggatgaa gaagctgctg ctatttggtc aaagtttctt
     3061 tctcccgaaa gaattattaa tggaagtaaa aaggataatt tttgggaaat gggcgaagtg
     3121 ggaccttgtg gaccttgttc tgaaattcat attgatattc gtaaagatga agaacgattg
     3181 aaaactgatg ccttgacttt aatcaataaa aatcatccac aggtaattga aatgtggaat
     3241 attgttttta tgcagtacaa tcgaaaagcc aatggttcat taatggaatt gcctgcaaaa
     3301 gtaattgata caggaatggg ttttgagcgt ttgtgcatgg tagtacaggg aaaaacttcc
     3361 aattatgata cagatttgtt ccaacctatt attaaagaaa ttggacaaat ttgcaaagct
     3421 gaatatgggc atgatgaaaa aattgatgta gcaatgcgag tagttgctga tcatatcaga
     3481 gccatagctt tttctattgc agatggacaa cttccttcta acaccaaagc aggttatgta
     3541 attcggcgga ttttacgtcg cgctttacga tatggttata ctttcctaca tcaacgttct
     3601 ccttttttat tcaagctggt ggatgttctt tgccaaacaa tgggctcagc ttatccagaa
     3661 ttgatagatc agaaatcttt gattgaaaaa gttatcaaag aagaagagga atccttttta
     3721 cggacgcttg agacaggaat tcagttgctg gaaaaaatga tagaaaaagc tataaatgaa
     3781 aaaaagaaag aaatctctgg caaggacgct tttatgctat atgatacttt tggatttccc
     3841 attgatttaa cagaattaat tttacgtgaa aataatctta gcgttaatat gaatgaattt
     3901 aatgctgaaa tggaaaaaca aaaagaacgc gctcggaatg ccgctaatgt agaaacagga
     3961 gattggatta ttcttcgtga aggggaaagt caatttgtgg gatatgacgt aacttcttgt
     4021 aatacagaaa ttttacgcta tcgaaaagta aagcaaaaaa ataatgagtt ttatcaatta
     4081 attctttcca aaactccttt ctatgctgaa atgggagggc aagttggcga ttcaggttgg
     4141 ttgctttgtg gtgaagacaa aattgaaatt ctcgatacaa aaaaagaaaa taatctttct
     4201 gttcatattg taaaacgact tcctgcaaat ttgacggata ggtttattgc ttctgtcgat
     4261 atagaaaaac ggttggcaac ttcacgaaat cactcagcta ctcacttgct gcatgaagct
     4321 ttgcggcagg ttttaggaac acatgtggag caaaaaggct cttatgtagg acctgaagga
     4381 ttgcgttttg atttttctca ttatcagaaa ttgacaaaaa aagagataag agcagtagag
     4441 gcaattgcta atcgtaaaat tagagaaaat attcctttga atgaacaacg tgagattcct
     4501 attcaaaaag ctcgtgaaat gggagcaatg gcactttttg gagaaaaata tgatgaaaca
     4561 gtacgccttg ttcaatttgg tacttcgctg gaactttgtg gaggaactca tgtaaaggct
     4621 actggggaaa ttggtatggt tagaataatt tccgaaactt ccatagctgc aggaattcgt
     4681 cgtatcgagg caattacagg tgaggctgta gaaaagctta ttgacactca acaagatctt
     4741 ttgaatgaag caaaagaatt attaaataat tctcctgatt tgatcactgc tattcgtaaa
     4801 ttgttagaag aaaatagaga attaaaaaaa ctggttaatg aatacaatga agaaaaactt
     4861 ttgcaacttc gtgataaatt attaagggaa gctgaaattg ttgagggtat tacagtagtt
     4921 tgttatgaag gaaatttaac atcagatcaa gcaaaagcat tggcttttca aattaagaat
     4981 cttcagaatt ctaatttatt ttttatagca ggaaatgtac atgaagggaa accttcttta
     5041 actgtattga ttaccgaaga tttagtaaaa aaagggttta atgccatcga tattgttcgt
     5101 aaggcagcaa aagaaattca aggtagtggt ggcggacaac ctttttttgc cgttgcagga
     5161 ggtaaaaatc cgcagggaat ccataaagca gttgctttga caagagagtt aataaagaag
     5221 gaataaaatt ttttgaaatt tttttacgat ttatttgttt ttgctttaat ttttttttat
     5281 ttttgccaaa tcaaatgcga taacaaacaa cagataatga atcatcgtta ttttacatat
     5341 ttttattttt acttttactt taaaaagtaa gagcaggata attattatgt aaaattacga
     5401 atgaaagttt ttagtttaat catatagaga gtcctgctca acatgctgag caggattttt
     5461 tatttataat tttaatcttt tgaatatgaa aagagtagct attcagggca ttagtggagc
     5521 atttcatgaa attgctgcaa gagagtattt taaaaacgaa gaaattgaaa ttgtaccttg
     5581 taatactttt aaggatgttt tcagtgctat agaaaatgat aaaacacttt tgggaattat
     5641 agccattgaa aatactattg caggaagttt attgcaaaat cataatttat tacgcgaaag
     5701 tgaatgtctt attgtgggag aacaaaaatt acatattgaa catcatttag tagcattagc
     5761 cgggcagcaa ttggaagatt tagaagaagt gcattctcat ccgattgctt tgatgcaatg
     5821 tgaagaattt ttagaatccc ataagcattt gaaggcagtg gaaagtgaag atactgcttt
     5881 atctgcaaaa gaaattgttg attatcaaat ttataaacgg gcagctattt gcagtcttta
     5941 tgcagctcaa aaatatgggc ttgaagttat agaaagaaac atagaaacca ataaaaaaaa
     6001 ttccacccgc tttttaattg ttgccgagcc gacacttgct ttggaaatga ccagaaatga
     6061 taaaattaat aaagcttctc tggtatttgc attgcctcat ttagaaggaa gtctttcaca
     6121 ggtactttcc attttatctt tttataacat gaatctcacg aaaattcaat cgttgcccat
     6181 tataggcaga gaatgggaat atcaatttta cattgatctc atttttaatg attattctcg
     6241 atacaaacag gcaatagatg ctattcttcc tttgattgat gaatttcaga ttttgggaga
     6301 gtaccacgta gatatttaaa atttataaac caatttagat catttataat tacagtaatt
     6361 ttttaaaata gtacaaatga atatacagcc agcaaaacgt ctaaataatg tttcagaata
     6421 ttatttttcg gccaaattaa gagaaataga tcaaatgaat gctgcaggga aaaaggtaat
     6481 taatttaggc attggaagtc ctgatttgat gccttctcca caaactattg aagctctttg
     6541 tcaagaagct cataagccaa acgttcatgg ttaccagagt tataatggga ttcctgaatt
     6601 acgaaatgga tttgcagaat ggtataaaaa atggtttcac gtggatttga atcctgataa
     6661 tgaaattcaa cctttaattg gttcgaaaga aggaatttta cacatcacat tggcttttgt
     6721 aaatcctggc gatagcgttc tggtgcccaa tcctggttat cctacttata cttctgtgag
     6781 caagttatgt gaagcaaatg taattcctta tgatttgaat gaaaataata attggcaacc
     6841 tgattttgaa aaacttgaga aaatggattt aaccaatgta aagttgatgt gggtcaatta
     6901 tccaaacatg ccgacaggcg ccaatgccag tgtggaatta tttgaaaaat tggttgattt
     6961 tagcaaaagg cataacattg taatttgtaa tgataatcct tatagcttta ttttaaatga
     7021 taaaaaactg agcattcttt ctattccagg agcaaaagat atttgtattg aaatgaattc
     7081 gatgagtaaa tcccataata tggccggatg gcgtattgct atgttggctt ccaatgctca
     7141 atttgtgcaa tgggtgctga aagtaaaatc gaatgtagat tcaggaatgt ttcgtcccat
     7201 gatgatagca gcagctactg ctttaaaaaa ttcagaagaa tggcacaacg aaatgaataa
     7261 aatttatgcc cgacgtcaac aacttgcttt taaaattatg gaccaattag gttgcacgta
     7321 tgatcccaag caggttggat tatttgtatg ggggaaaatt cctgctcact atgccgatgc
     7381 tggcgaatta gctgacgaaa tattatataa aaagaatgta tttattactc ctggttttat
     7441 tttcggtgat aaagggaaac gatatattcg gatatctttg tgttgtgcag aacctgtttt
     7501 agaggaagtt ttaaaaagat tgagttagat aaaaaataaa attttgtaat agttggataa
     7561 attgttaaaa taatatttac tatggaattt gaatcaattt tattaccggg tatagaagcc
     7621 aaacgaccaa ttgttattgc aggtccttgt agtgctgaaa ctgnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnggtatag aagccaaacg accaattgtt attgcaggtc
     7801 cttgtagtgc tgaaactgaa gagcaggtaa tagaaacagc acgtgaactg gcttcttttg
     7861 gaataaagat attccgagct ggagtttgga aaccccgcac caaacctggt ggttttgaag
     7921 gggtaggagt gaagggatta ccatggctta agcgtgtgaa aacagagttc ggtatgtaca
     7981 ccactgtaga agtagcaaat gctaaacatg ttcatgaagc tttggctaat gatgtagata
     8041 ttttatggat tggtgcacgt acttcggcaa atccatttgc tgttcaagaa attgctgatg
     8101 ctttagaagg aattgatatt cctgtactaa ttaaaaatcc aattaaccct gatttagatt
     8161 tgtggattgg agctattgaa cggatttaca atgcaggaat taggcgaatc gctgctgtcc
     8221 atcgtggatt tagttcttat gataaaaaaa tttatcgaaa cttgcctcag tggcacattc
     8281 caatggaatt acatagacaa attcctaatt tgcccttgtt ttgtgatccc agccatattg
     8341 gtgggaaacg tgaactaatt ggtcccttga gtcagcaagc aatggatttg aatttcgatg
     8401 ggctaatgat tgaatcgcat tgtaatcctg atgaagcctg gagtgatgcg gctcaacaag
     8461 ttacgcccaa aattttagac tttattttaa attcattggt cattcgtgaa accacccaaa
     8521 ccactgaaaa tttaactgct cttcggcgtc aaatagatga gcttgataat gatttacttc
     8581 aattattggc taaaagaatg agagtttcag aagaaattgg agtgtataaa aaagaacaca
     8641 atatgcctat tttccagcct gaacgttatg atgaaatgtt aaaggacaga gtgaaacagg
     8701 ctattcaaat ggggatggat ggcgatttta tgaaaaatgt attgattgct attcatgaag
     8761 aatcgattcg tcaccaaatg gtaattatgg aaaaatctga tgccacttta aataattcga
     8821 attcttcaac aatccaatga attattaaag aaaagtaaag atcgatccta aaaatttatt
     8881 gaaaagtttt tatcttcttt cagaatgttt ttgattatta gaatgttctg ctgcatttcc
     8941 acgtgacgat cgagttgaag acttttgtga aggtgaggct gaagattcag cagaacgtct
     9001 gtttgtacca gaaacctttc tttctttttg aggaggatta tttatttctt ttcccccaga
     9061 agaattttga tttcttcttt caacatttcc actgctgctt gatgactgta aattacgaga
     9121 agaggttttt ctgtaatttt cattagtctg atttaccttt cgacgatttg cttctgatga
     9181 tgttcgacga gcagaattaa attcagcttt gttggctata cctttgttgc gattgttaaa
     9241 acttttttct ggatgttttc gctcataatc atttcgccga acagtttgat gaagttttac
     9301 ataaacatcg ttgtgataga tattaaccgt tgtgtgataa taaaatttac agtcacgtgg
     9361 tatatacctg tgaatatgtc ccatgtaaat atttactttc acaggtggac gagggtgata
     9421 aaattttgga taatattccc aatagtaagg tgaccgccaa ggacgataat ggctactcca
     9481 gaagaatgta aaaattactg gctctcgcac aaaaacaggt tcaataatat aattcactcc
     9541 atataaatac ggatttccaa taattcgaac ataaatttga ggattgttac gcttctcaac
     9601 tactatggtt gccacatcct gatatgtgtc tctatccaat actgcctgaa ttacaacaag
     9661 atgcaaatct ctttcagaag tttctaccac acgtaaataa tcaatccagc catcattatt
     9721 caaatcaaga tttgaaattt gataacgttc atcattcaat ctcatttcaa aatcttttaa
     9781 attttttgat tccgcaaaaa tagaaacaac tgctctcaaa tcgagattat cgcttatatc
     9841 gtaattttga gcttctattg taaaagctct ttgtgcgggt gcctgagcag caactaaaag
     9901 taaaattgct aaactcaata tttttatttt catacctgga ttttttaagg atcaataaat
     9961 caagtttttt gttttaaggt acttatgcat gggataataa taaattcaaa acatatgcca
    10021 aacttttttg agcttatatt taactataca tatttctgaa tatcaaaatt atatgtaaat
    10081 attttttatt ttttaaaaca taacactaaa aatggtattt ttaaagttag gaaagaagag
    10141 aataagctta aatcttataa attgttcatt atgttattgt gttaagacta gcaatcatca
    10201 gcctatttgg aatcgatttt tctcctgtaa ttttacctaa gaaatttttt cacgaaaaat
    10261 aatttatgtt tatatgttat atttactcat tctcttttta aaatgtcgaa gtaactcgga
    10321 aatattttat aattttgtat aataattggt tgaatataaa aaatatgagt attttaaatt
    10381 ttttcaataa agataagaaa gaaacgctcg aaaaggggtt agaaaaaaca agagaaagtg
    10441 ttttttcaaa aatttcgaga gctattgcag gcaaatcaaa agtagatgat gaagtgctgg
    10501 atgaattaga ggaagtacta atcacttctg atgtgggcgt agaaacgact ttgcgaatta
    10561 ttgataggat cgaaaaaaga gtcgcaagag ataaatatgt aaacaccaat gagttaaaca
    10621 ctattttaaa ggaagaaatt gctgctttac ttgcagaaaa taatattgaa tctgtggctg
    10681 acttttctgc taatttgccc gctaaacctt atgtgataat ggtggtaggt gtgaatggag
    10741 taggtaaaac gacaacgatt gctaaattgg cttatcaata tataaaagag ggtaaaaaag
    10801 tttttcttgg agctgctgat acttttagag cggcggctat cgaacaactt tctatttggg
    10861 gtgaccgagt gggtgctcac gtagtgaagc aaaaaatggg agcagatcct gcgtcggtta
    10921 cttatgatac cattgcttct gccatggcta atgatgcaga tgtagtaatt atcgatacgg
    10981 ctggaagatt gcataacaaa attaatttaa tgaatgaatt aacgaaaatc cgaaacgtta
    11041 tgcaaaaagt tatgccagga gcacctcacg aagtgttatt ggtgctggat ggttcaactg
    11101 gacaaaatgc ttttgaacag gcaaagcaat ttacaaaagc aacagatgtc actgctttgg
    11161 caataaccaa actggatggt acggcgaaag gtggagttgt attaggcatt tccgatcaat
    11221 ttaaaatccc tgtaaaatat attggattgg gtgaaggttt agaggatttg caagtatttg
    11281 ataaaatggc atttgtggaa tcattattcg aataaatctt tatttatcaa caaattaaat
    11341 aaagattttt gatctccagt aagtgactat catgaaaaag aaagacacga ttgatattat
    11401 tacattgggt tgttctaaaa atttggtaga ttctgagttt ttaatgcgtc aatttgaagc
    11461 tttgggttac cgagttcgtc atgatgcgga agttcccgat ggcaatattg tgattgtaaa
    11521 tacttgtgga tttattgccg acgcgaagga agaaagtatt tatactattt tgcagtttgc
    11581 aaaacagcga aaagaaaaca aaattgacaa attgtttgtg atgggatgtc tatcgcaacg
    11641 ttatttaaaa gaattgcagg aagaaattcc agaagtggat aaattttatg gaaagtttga
    11701 ttatgtggga attttaaaag aacttggcga aaattatcaa tcgcatttgc gtctggaaag
    11761 aactttaacc acacctgctc attatgctta tgttaagatt gctgaaggtt gcaaccgaac
    11821 gtgttcatat tgtgccattc ctttgatgac aggcaaatat caatcacgtc cgatggaaga
    11881 tatagaaaag gaagttaaaa tattagtttc aaaaggagta aaggaattgc aattaattgc
    11941 tcaagattta tctgattatg gggaagatct ctacaaaacc tccaaattag cagaattaat
    12001 cgagcgtctt tcagatattc aaggagtgga atggattcgt ttacattatg cttatcctgt
    12061 acgcttccca tttgatgtgc tgaaagtaat gagagaaaga gataatgtat gtaaatattt
    12121 agatatttct ttccagcata ttagcgataa aatgttgaag ttgatgagaa gaaatatcac
    12181 aaaaaagcaa acttatgaat taattgaccg aattagagaa gaagtgccag gtattcattt
    12241 gcgaacaacc ttaatggtag gacatccagg tgaaacggaa gaagatattc aagagttaaa
    12301 ggaatttata agaaaagaaa aatttgaacg tttgggagct tttacttatt cgcacgaaga
    12361 aggaactttt gcttatcaaa attatgaaga tgaaatttct gaaaaagaaa aattgcaacg
    12421 tttagataaa attatggaat tacagcaaca gatttccttt gaattgaatc aatctaaaat
    12481 tgggaaagta tttaaagtaa taattgatag ggtagaagga gaatattata taggacgaac
    12541 ggaatttgat tcgccagaag tggatccaga agttttaatt cctatttcaa acaaaaacat
    12601 taaaattgga gaattctcaa a
//
LOCUS       DAOTKS010000005        12373 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_5541_length_12373_cov_4.612518, whole genome shotgun sequence.
ACCESSION   DAOTKS010000005 DAOTKS010000000
VERSION     DAOTKS010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12373
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5541_length_12373_cov_4.612518"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..914
                     /gene="udk"
                     /locus_tag="P5236_00220"
     CDS             279..914
                     /gene="udk"
                     /locus_tag="P5236_00220"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007759125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HRU62824.1"
                     /translation="MLIIGIAGGTGSGKTTVVQKIMERLPKSEVVVLPQDAYYRDTSF
                     LPLEERLEINFDHPDSIEFELLIKHLKDLKEGKSIEMPIYSYLTCSRQNETITIHPCD
                     VVMVEGILVLSQPELRKLMNLKVFVDADADDRLIRVIYRDITERGRTVNKVIERYEKT
                     VKPMHEEFIEPTKRFADLIIPQGGNNNIAIDILTKYIENNLRLNHNSNLEN"
     gene            994..1731
                     /locus_tag="P5236_00225"
     CDS             994..1731
                     /locus_tag="P5236_00225"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRU62825.1"
                     /translation="MHLNELKKIMFLDIETVPQVANFSDLPDELAHLWEEKFHHLRKH
                     LPEKYNEKTTASEAFNTSAGIYSEFGKIICIAVGFIHFKEEEMFLRTKYFINEDEKEL
                     LMEFSQLIEKFGSSKENRLCGHNIKEFDVPYICRRMLINNLPLPSIFQLSGKKPWESS
                     LIDTMELWKFGDYKNFTSLKLLTAVFNIPTPKDDIDGSEVAQVYYKEKNIQRIAQYCT
                     KDVIATAQVFLRLNGFPPISAENIENL"
     gene            1741..3117
                     /locus_tag="P5236_00230"
     CDS             1741..3117
                     /locus_tag="P5236_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRU62826.1"
                     /translation="MKNLYLIIFNLFLILTFVSCKKNEIDKRAQISNRDLEQIILSDT
                     LKVTTMYGSTSYFLFRDEDMGFDYEMAENLADFLKVKLKVELANSEPQMIQLLKDGKV
                     DIVAYNIPETKELKKDFQYVFQQPRNSMVIVQRMGRNALSSVVELKDKKVYVKNNSVF
                     HQRLKDLNNEIGGGIQIITVPDSITNDELIEMVANSKIDFTVAYRDKALLHQSYFRRL
                     DCRLTIGFEQNNGWLIRKTSKNLLTAIKEWESLQKTQDKKNRLLTKYWNKSPYFVIHK
                     IKIPKGAISPYDDLFKKYAPTIGWDWRLLAALAFHESRFDSAAISRVGALGLMQLMPQ
                     TAAQFGLDKSDIFNPEKNIEASIQYIKSLNMAFSQVVDKNERIKFIIASYNSGPSHIL
                     DAMALAQKYGKNPYIWFDNVEVYLLEKRNPEYYNDPVVKNGYYRGTETVRYVENVLDT
                     YEKYLKRK"
     gene            3217..3435
                     /gene="tatA"
                     /locus_tag="P5236_00235"
     CDS             3217..3435
                     /gene="tatA"
                     /locus_tag="P5236_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRU62827.1"
                     /translation="MNPLLFNLNGWEIPIILLVILLLFGGKKIPEFMSGLGKGIRSFK
                     KGLNDIEEEIKADPDEKKPNDNKSSSSK"
     gene            3532..4341
                     /gene="tatC"
                     /locus_tag="P5236_00240"
     CDS             3532..4341
                     /gene="tatC"
                     /locus_tag="P5236_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRU62828.1"
                     /translation="MKEKEMSFWEHLEEFRWTLIRIIIAIAITTIIGLIFIPKIFDSV
                     ILAPCSSNFVLYRFFEKLSGYFPFLLNYSSSSFHVKILNINMATQFMTYMSASLAFGF
                     IFSIPYVLYELWKFISPALYEKETKGVKKAFLLGGVMFLIGCLLGYFIIFPLIFRFLI
                     TFKLSNNIENMISLDSYMRNFYTLILLMGVVFELPLLFWLLSNIGLITRSFFKKYRRY
                     AIIVSLVLAALITPSGDPFSLFVVAIPLYFLWEFSALLVKKDNFEQDILTL"
     gene            4390..5028
                     /locus_tag="P5236_00245"
     CDS             4390..5028
                     /locus_tag="P5236_00245"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HRU62829.1"
                     /translation="MENDFIKTVTHTIKHWYIPLIIGILFIVIGIFCFISPMVSYFTF
                     SLIFSVSFLFSGILEIIFSITNRKETDNWGWTLAFGIITFIIGLLLVLNPRITMLSLP
                     YFIGFLMLFRSIFAMSLAIDLKNYYVLNWGYLLAIAILGIIFSFILLLNPLFGGIAIT
                     LLLGFTFLASGCYSIYLSFELKRLKKYADKIPDELKERYESIKREIQEKIWE"
     gene            complement(5129..6379)
                     /locus_tag="P5236_00250"
     CDS             complement(5129..6379)
                     /locus_tag="P5236_00250"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HRU62830.1"
                     /translation="MKRNLFLIPLFFVMSLHSQKIYHTQILHPTIKTLQIGLSGQNYS
                     LPLIQLNSKDILEIQFDELSHNIHNYTYKVFHCNADWTLSNLSTNEYLEGFTTGNIYD
                     YALSQNTTYQYTHYRFILPNEEMNFKISGNYMVMIYEENKMENPVAIACFSVVEPQVN
                     IIARLRANTDIELSGRFQQLDIEVGLNGYRLQDPQSEIKMVIRQNNRIDNQVTNILPT
                     YLSTEKLSYINQKALIFEGGNEYRHFDMSSIYVVGEGLDTIRFNKTFYDVYLKEDVPS
                     TSKNYLSQPDLNGKFIIHYQEAFDDINTEADYMQVHFSLYTGQPYFDGQVYIGGEYNY
                     NLLNETSQMQYNFAEGRYNKTILLKQGGYNYQYWFLPKGGKKASVEKIEGSYWQTNNE
                     YTIYVYHRPWGGRYDKLIGVKVMN"
     gene            6683..8065
                     /locus_tag="P5236_00255"
     CDS             6683..8065
                     /locus_tag="P5236_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HRU62831.1"
                     /translation="MANLIKTTRGLDIPIEGEAQKTLGGTVISDVVALVPEYYHGVVP
                     KMLVKAGDEVKAGSPIFHDKTFDTMNFVSPVSGKVLAINRGERRKILSIAIERDSKIQ
                     YVPFKIKPLSETTGEEVKAQLLNAGLWPFIKQRPYDIIADPNKSPKAIFISCFDSSPL
                     APDYNFILKGQFGDFQTGIDALSKLTKGKIHLGIKPTKDFNDFRSVRNVEITEYEGPH
                     PIGNVGVQINHVDPINKGEIVWTINVQHVLFIGRFFNKGIVDLSYTLALTGPCVVQPQ
                     YYRTLVGANIEPFIKGNVYKGLILRYITGNVLSGIKIEENEWLNPYASQIFVLEEGSD
                     QHEFLGWAMPRIHKFSASNLYFSKIFQKKWFQKFFGKIKYQYDARLLGGKRAIIMSNE
                     YDKVLPMDILPEYLFKAMITGNIEKMEALGGYEIAPEDVALCEFICTSKLPLQLIVRD
                     ALDTMKKELE"
     gene            8108..9319
                     /locus_tag="P5236_00260"
     CDS             8108..9319
                     /locus_tag="P5236_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HRU62832.1"
                     /translation="MKNALRNFMDKVKPHFEKGGKFEKLHSVYDGFETFLFVPNTTAP
                     KYGSHIHDPNDTKRTMITVVIALIPALLFGMYNTGYQHYLAIGVNANFWITFLFGFLA
                     VLPYIIVSYVVGLGIEFTVAQIKGEEIQEGFLVTGFLIPLILPINTPLWMVAIATAFA
                     VIFAKEVFGGTGMNIFNVALVTRAFLFFAYPTHMTGDSVWVRTGSALGFGGGKAIDAF
                     SGATPLGQIATSTNSLPHITDTFGNTLSFWDGFIGLIPGSVGETSKIAILLGAILLLV
                     TGVASWKTMLSVFLGGAFMGIIFNLFGPDTASANFPWYCHLVFGGFAFGAVFMATDPV
                     TSARTEKGKWIYGFLIGCMAIIIRVLNPGYAEGMMLAILLMNIFAPLIDYYVVEANVK
                     QRNKRITKPVQ"
     gene            9367..10089
                     /gene="nqrC"
                     /locus_tag="P5236_00265"
     CDS             9367..10089
                     /gene="nqrC"
                     /locus_tag="P5236_00265"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HRU62833.1"
                     /translation="MDTNSNSYTLIYATIVVVIVAFMLAFVSGALKEKQNANIELDKK
                     RQILSSLQIDTKGKDVASLYDQYIVKGLVINYKGEILSESKKEAFNIDFAKELTKKLE
                     DRRLPLYIANVDGETKYILTLRGTGLWGPIWGYLALNDDKNTIYGVYFSHASETPGLG
                     ASIAELPFQKQFIGKHILNDKNEFVSIAVMKAGKKAEGKDQVDAISGGTITSKGVEAM
                     LLNCIGQYVQFLQQPTTGGMNQ"
     gene            10086..10718
                     /locus_tag="P5236_00270"
     CDS             10086..10718
                     /locus_tag="P5236_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HRU62834.1"
                     /translation="MSKLFSRKSKQAFLWPLSKNNPIMVQILGICSALAVTAKLKPAL
                     VMGISVTIIVAFSNVIVSLIRDTIPNRIRIIIQLVVVAALVTIVSEILKAYVYDVSVQ
                     LSVYVGLIITNCILMGRLEAYAMSNKPWDSFLDGLGNGLGYAIILVIVSFFRELLGSG
                     TLFDIRVIPASFYEAGYMNNGLMLMPPMALILIACIVWIHRYHNKELQEK"
     gene            10743..11360
                     /gene="nqrE"
                     /locus_tag="P5236_00275"
     CDS             10743..11360
                     /gene="nqrE"
                     /locus_tag="P5236_00275"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HRU62835.1"
                     /translation="MENVFNIFVKSIFVDNMIFAYFLGMCSYVAVSKNVKTSMGLGIA
                     VIFVLLITVPVNYLLENYVLKEGSLKWLSPELANLDLSYLSFILFIAVIAGIVQFVEM
                     VVEKFLPSLYNSLGIFLPLIAVNCAIMGACLFMQQRAYQNIGEATAYALGSGIGWWLA
                     IIGLAAIREKMEYSDVPAPLKGLGITFITVGLMGIAFMCFSGLKL"
     gene            11433..>12373
                     /gene="nqrF"
                     /locus_tag="P5236_00280"
     CDS             11433..>12373
                     /gene="nqrF"
                     /locus_tag="P5236_00280"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HRU62836.1"
                     /translation="MDTVNLVASLVVFLVVILLLVIILLVAKQYLISSGTVTITINEN
                     KKLEVPAGGTLLNTLAMQQIFLPSACGGKGTCAQCKCQVFEGGGEILPTEISHFTRKE
                     IKEKWRLSCQVKVKENISIKIPPSILSVKKWECKVISNKNVATFIKELIVKLPEGERL
                     DFIPGSYIQIDIPKYNTIKFSDFDIDERFRSDWDKMKLWDLSCDNDEETMRAYSMANY
                     PAEGKDIVMLNVRITTPPIDKSTGTFMDVKPGIASTYIFNLKPGDRVKVSGPFGEFHP
                     IINSKREMLYIGGGAGMAPLRSHILHLLKTLQIRDRK"
BASE COUNT         4132 a         1746 c         2112 g         4383 t
ORIGIN      
        1 cggagatttt tcaaaattga aagttgcata ttcatggtta ttaaaattta aaaaagattt
       61 ttcaaattta catttttatt ctttcaaaag aaaattattt gaaacaattt aaaaagcgga
      121 ttagaaaaaa tttaatatat actaaatgtt ctttattgct aaatagttaa ttatcaatta
      181 tatatatgtt ttatttatta aaatagaaat attttatgta tttttgaaaa ataaaagtat
      241 aaaattttat tttcttaaaa atcaatcatt aatttactat gttaattatt gggattgcag
      301 gaggtaccgg ttctggcaaa acaacagtgg tacaaaaaat tatggaaaga ttgccgaaaa
      361 gtgaagtagt tgttttgcca caagatgcat attatcgtga taccagtttt ttacctttag
      421 aagaaagatt ggaaattaat tttgatcatc cggattcaat agaattcgag ttattgataa
      481 aacatttaaa ggatttgaaa gaaggaaaaa gtattgaaat gcccatttat tcttatttaa
      541 cctgttcccg ccaaaatgaa acaattacca ttcatccttg cgatgtggta atggtagaag
      601 ggattttggt actttcacaa cccgaattaa gaaaactcat gaatttgaaa gtttttgtag
      661 atgccgatgc agatgaccga ttgataagag tgatttatcg ggatatcacg gaacgtggtc
      721 ggacagttaa taaggtaata gaacgatatg aaaaaacagt aaaacctatg catgaagaat
      781 ttattgagcc aactaagcgc tttgccgatt tgattattcc tcagggagga aataacaata
      841 ttgctattga tattctgaca aaatatattg aaaacaattt aaggttgaac cataattcta
      901 atttagaaaa ctaaaaaaat actatttctc catcgatttt caacaaatta aattttccat
      961 agaaaataat tcattttata aatattaatt gatatgcatt tgaatgaatt aaaaaagatt
     1021 atgtttctgg atatagaaac agtaccacaa gtagcaaatt tttctgattt gcccgatgag
     1081 ttggctcatt tatgggaaga aaaatttcat catttgcgaa aacatttacc agaaaaatat
     1141 aacgagaaaa ccactgcttc cgaagctttc aatacaagtg ctggtatcta ttcagagttt
     1201 ggaaaaatta tctgtattgc agtaggtttt atccatttta aagaagaaga gatgttttta
     1261 cgaaccaaat attttatcaa tgaggatgaa aaagaacttt taatggaatt ttcacaactg
     1321 attgaaaaat ttggctcatc gaaagaaaac cgactttgtg gacacaacat caaggaattt
     1381 gatgttccct atatttgtcg aaggatgctg atcaataatt tgcctttacc atcgatattt
     1441 caactttcag gcaagaaacc ctgggaaagt agcctaattg acacaatgga actctggaaa
     1501 tttggtgatt ataaaaattt tacgtccctg aaattactta cggcggtttt taatatccct
     1561 actccgaagg acgatattga tggaagtgaa gtagctcagg tttattataa agaaaagaat
     1621 attcaacgta ttgcccaata ctgtaccaaa gatgtaattg caacagcaca ggttttcttg
     1681 cgattaaatg gctttccgcc tatttcagct gaaaatattg aaaacctcta atttgtttct
     1741 atgaaaaatc tttatctgat tattttcaat ttatttctta tacttacatt cgtttcctgc
     1801 aaaaaaaacg aaattgataa aagagctcag ataagcaata gagatctgga acaaattatt
     1861 ctttccgata cccttaaagt caccacaatg tatggttcta cctcttattt tctctttcgt
     1921 gatgaggata tgggatttga ttatgaaatg gcagaaaatt tagcagattt tctaaaagtg
     1981 aagctgaaag tagagcttgc caatagcgaa cctcaaatga ttcaactttt aaaagatggc
     2041 aaagtagata tagtggctta taacatcccc gaaacaaaag aattaaaaaa agactttcaa
     2101 tatgtatttc aacagccacg caacagtatg gtgattgttc aaagaatggg tcgcaatgct
     2161 ctttccagtg ttgttgaatt aaaagataaa aaagtgtatg taaaaaataa tagtgttttt
     2221 catcaacgtt tgaaagattt gaataacgaa attggcggag gaatacaaat tataactgtt
     2281 cctgattcaa taaccaatga tgaattgatt gaaatggttg ccaatagcaa aatagatttt
     2341 acagtagctt atcgagataa agcgttactt catcagtctt attttagacg tcttgattgt
     2401 cgcttaacaa taggttttga acaaaataat ggttggttga tacgaaaaac cagtaaaaat
     2461 cttttgacag ctatcaaaga atgggaaagt ctacaaaaga cgcaagataa aaaaaataga
     2521 ttattaacta aatattggaa taaaagtcct tattttgtca ttcataaaat aaaaatacca
     2581 aaaggagcaa ttagtcctta tgatgattta tttaagaaat atgcacctac cattggttgg
     2641 gattggcgat tattggccgc tcttgctttt catgaatcgc gttttgatag tgcagctatt
     2701 tcacgagtag gagctctcgg actgatgcag ttaatgcctc aaactgctgc ccaatttggc
     2761 ttagacaaaa gtgatatttt taatcctgaa aaaaatattg aagcctctat tcaatacatt
     2821 aaaagtttaa acatggcttt tagtcaggtt gtcgacaaaa atgaacgtat caaatttatt
     2881 atagcaagtt ataattctgg cccttctcat atacttgatg ctatggcttt ggctcaaaaa
     2941 tatggtaaaa atccgtatat ctggtttgat aatgtagaag tatatttact tgaaaaaaga
     3001 aatccagaat attacaatga tcctgtggta aaaaatggtt attatagagg aactgaaact
     3061 gttcgatacg ttgagaatgt attagatacc tatgaaaagt atcttaaacg taaataaata
     3121 aaattctcgt tttttatatt ttataaactt tatcttaatt ttattgctgt agagtcgaaa
     3181 taattatatt tttgtattaa ataaaaacat tagaccatga atcctttact ttttaattta
     3241 aatggttggg agataccgat tattcttttg gtaattcttc ttttgttcgg cggaaaaaaa
     3301 attcctgagt tcatgagtgg cttgggaaaa ggtattcgca gctttaaaaa aggtttaaat
     3361 gatattgaag aagaaataaa agctgacccg gacgagaaga aacccaatga taataaatca
     3421 tcttcttcaa aataatttga tttgaatttt ctttctctaa gaaaataata aaagccaagc
     3481 aaaatttttt tgacgcttgt ggtattcact attaaatttc aggataaata gatgaaagaa
     3541 aaagaaatgt ccttctggga acatttagaa gaattccggt ggactttgat tagaattatt
     3601 atagctattg ctattacaac catcataggt ttgattttta ttcctaaaat tttcgattct
     3661 gttatcctgg ctccgtgttc ttcaaatttc gttctttaca gattttttga aaaattaagt
     3721 ggttattttc cttttctatt gaattattcc tcttcctctt ttcatgttaa gatattgaac
     3781 atcaatatgg caacacaatt catgacatat atgtctgctt cattggcttt tggatttata
     3841 ttttcaattc catatgttct ttatgagttg tggaaattta tcagcccagc actttatgaa
     3901 aaggaaacca aaggagtaaa aaaagctttt ctattgggag gagtgatgtt tctgatagga
     3961 tgtttattag gttattttat tattttccca ttaatattca gatttttaat tacttttaaa
     4021 ttgagcaaca acattgaaaa tatgatttct ttagattcct atatgcgaaa tttttatacc
     4081 ctcattttgt taatgggcgt tgtttttgaa cttccccttt tattctggtt attgtcaaac
     4141 atcggactta ttacccgttc cttttttaaa aaatatcgac gatatgctat tattgtctca
     4201 ttggtgctgg ctgctttaat tacaccttct ggcgatccgt tttctctttt cgttgtggca
     4261 attccacttt attttttatg ggaatttagt gctttattgg tgaagaaaga taattttgaa
     4321 caagatattt taactttgta aaatgttata tttctaaaaa tcatatatta acttttaaaa
     4381 ctgataatca tggaaaatga ctttattaaa accgtcactc ataccattaa acattggtat
     4441 attcctttaa ttattggaat cttgtttatt gtaatcggaa ttttttgttt tatatcgcca
     4501 atggtttctt attttacttt ttcattgatt ttctcagtct cttttctttt ttctggtatt
     4561 ttggaaatta ttttttctat tacaaatcga aaagaaactg ataattgggg ctggacattg
     4621 gcttttggaa ttattacttt cataattggt cttttgctgg tgcttaatcc acgtattacg
     4681 atgctttcat tgccttattt cataggcttt cttatgctgt ttcgctcaat ttttgccatg
     4741 agcctggcaa tagatttaaa aaattattat gttctgaatt ggggatattt attagctatt
     4801 gctattctgg gaattatctt ttcctttatt ttgctgctca atcctttgtt tggaggaatt
     4861 gcgattactt tattgttagg ttttactttt ttagcaagtg gttgctacag tatttatctt
     4921 tcttttgaat taaagcggct gaaaaaatat gcagataaaa ttccagacga attaaaagaa
     4981 agatatgaaa gcattaagcg agaaattcaa gaaaaaattt gggaataata tttaaaacta
     5041 ctcctgccct acctattctt acttcaaaat ataactttta gaagtaaggg tatttgttaa
     5101 tcatttatat ggaatatctt tcaattcgct aattcattac ttttacaccg ataagtttat
     5161 cataacgtcc tccccacgga cgatggtaaa cataaatggt atattcattg ttggtttgcc
     5221 aataactacc ttctattttt tctacagaag cttttttccc cccttttgga agaaaccaat
     5281 actgataatt ataacctcct tgcttgagta aaatagtctt attgtaacgt ccttcagcaa
     5341 aattgtactg catttgcgat gtttcattca gtaaattata attatactct ccaccaatgt
     5401 aaacttgtcc atcaaaataa ggctgacctg tatacaatga aaaatgaact tgcatataat
     5461 cagcttcagt attaatatca tcaaatgctt cttgataatg aataatgaat ttcccgttca
     5521 aatctggctg agataaataa tttttcgaag ttgatgggac atcttctttt aaataaacat
     5581 cataaaatgt tttattaaag cgaattgtat ctaagccttc accaacaaca taaatagatg
     5641 acatatcaaa atggcggtat tcattacctc cttcaaatat tagtgctttt tgattaatgt
     5701 aacttaattt ttcagtactt aaataagtag gcaaaatatt agttacttga ttatcaattc
     5761 tgttattttg ccttattacc attttaattt cagattgagg gtcttgtaaa cgatagccat
     5821 ttaatccaac ttcaatatcc aattgctgaa aacgtccact taactcaata tctgtatttg
     5881 cgcgaagtct cgcaataata tttacttggg gttccaccac tgaaaaacaa gctatcgcaa
     5941 caggattttc cattttgttt tcttcataga tcattaccat ataatttcct gatattttaa
     6001 aattcatttc ctcgtttggc aaaataaaac gataatgtgt atattgataa gtagtatttt
     6061 gtgacaaagc ataatcgtaa atgttgccag tagtaaatcc ttctaaatat tcattagtcg
     6121 ataaattcga aagtgtccaa tctgcattac aatgaaatac tttgtaagtg taattgtgaa
     6181 tattatgtga caattcatca aactgaattt caaggatatc tttactattt aattgtatga
     6241 ggggtaaact ataattttga cctgataatc caatttgtaa agttttaata gtgggatgca
     6301 aaatttgagt atgataaatt ttttgtgaat gtaaactcat cacaaaaaat aaagggatta
     6361 aaaataaatt tcgtttcatg agctataaaa attaaaaaac gtgctaagta gaaataaaac
     6421 aaattgtttg tacaatatta caaatatagc catgttattt gaatttttta ataaaacaat
     6481 aatataaatg gtttattttt ctattaataa ctaaattatt gataaataat aaataaatta
     6541 attaattata tgattttaat taatgaaata attctttaat aaaatgctga gtaaccaata
     6601 aaaaatgtgt aattttgcaa cgttttttaa ggtaaggcaa aatttcccta aaatagggat
     6661 taaatttaaa tttagaaatt ttatggctaa tctgataaaa actacgcggg gtcttgatat
     6721 tccaattgaa ggggaagctc aaaaaacatt gggcggcacc gttatttcag atgtagtggc
     6781 acttgtaccg gaatattatc atggcgtcgt tcctaaaatg ttagtaaaag ctggcgatga
     6841 agttaaagca ggttctccca tttttcatga caagactttt gatactatga attttgtttc
     6901 accagtgagt ggtaaagttt tggctattaa cagaggtgaa cgtagaaaga tattaagtat
     6961 cgctatcgaa cgagatagca aaattcaata tgttcctttt aaaataaagc ccttatctga
     7021 aacaactggt gaagaggtaa aagctcagtt attgaatgct ggattatggc ctttcatcaa
     7081 gcaacgtcct tatgatatta ttgccgaccc aaataaatca cctaaagcaa tttttatttc
     7141 ttgttttgat tcctcaccgc tggctccaga ttacaatttt attttaaaag gtcaattcgg
     7201 tgattttcag actggtattg atgctttaag caaacttacc aaaggaaaga ttcatcttgg
     7261 aataaaacca acgaaagatt ttaatgattt ccgctccgtt cgaaatgtag aaattactga
     7321 atatgaagga cctcatccta ttggcaatgt tggcgttcaa attaaccacg tcgatcctat
     7381 taacaaagga gaaatagttt ggactattaa tgttcagcat gtattgttta ttggaagatt
     7441 tttcaataaa gggattgtcg atttaagtta tacacttgct ttaaccggtc cttgtgtagt
     7501 tcaaccccaa tattatcgta ctcttgttgg tgctaacatc gaacctttta ttaaggggaa
     7561 tgtatataaa ggtcttattt tacgctatat tacaggaaat gtgctcagtg gaattaaaat
     7621 agaagaaaat gaatggttaa atccctatgc ttctcaaatt tttgttcttg aagaaggaag
     7681 tgatcaacat gaatttttag gttgggcaat gccaagaatc cataaattta gtgccagtaa
     7741 tttatatttt tcaaaaatat ttcaaaaaaa atggtttcaa aaattttttg gcaaaattaa
     7801 atatcaatat gatgcccgtt tattaggcgg taagcgtgcc attatcatgt ccaatgaata
     7861 tgataaagtt ttacctatgg atattttgcc agaatatctt tttaaagcca tgattacagg
     7921 aaatattgaa aaaatggaag cacttggagg atatgaaatc gctcctgaag atgtggcttt
     7981 atgtgaattt atttgtacat ctaaattgcc acttcaactt attgtgcgtg atgctttaga
     8041 tacaatgaaa aaagaacttg aataacacaa caacattaaa tttgcctcta aaggaaaaaa
     8101 tcgtgatatg aagaatgctt taagaaattt catggataaa gttaaacctc attttgaaaa
     8161 aggaggtaaa tttgaaaaac ttcattctgt atatgatggt tttgaaacct tcttatttgt
     8221 tcccaatact accgctccaa agtatggtag ccacatccat gatcctaatg atacaaaacg
     8281 taccatgata acagtggtga tagctcttat tccagctctt ttatttggga tgtacaatac
     8341 tggttatcaa cactatttag ctattggagt aaatgccaat ttttggataa cttttttgtt
     8401 tggcttttta gcagttttac cctatataat tgtttcttat gtagttggtt taggaattga
     8461 atttacggtg gctcaaataa aaggtgaaga aatccaagaa ggttttttgg taacaggatt
     8521 tcttattcct ttaattttac caatcaatac tccgttgtgg atggtagcta ttgcgacagc
     8581 ttttgctgta atttttgcaa aagaagtttt tggtggaact ggaatgaata tttttaatgt
     8641 agctttagtt acacgagctt ttttattctt tgcttatcca actcacatga caggtgactc
     8701 agtgtgggta cggacaggaa gtgccttagg ttttggtggc ggtaaagcaa ttgatgcttt
     8761 ttcaggtgct actccgctgg gacaaattgc tacttccaca aatagcctcc ctcatattac
     8821 cgatactttt ggcaatacat taagtttttg ggatggattt atcggattaa tccctggctc
     8881 ggtaggagaa acttctaaaa ttgccatttt gcttggggca atacttttgc tcgttactgg
     8941 tgtagcaagt tggaaaacta tgctttcagt atttttaggc ggtgctttta tgggaattat
     9001 ttttaatttg ttcggtcctg atacagcttc tgctaatttt ccatggtatt gtcatttagt
     9061 atttggcggt tttgcgtttg gtgcagtatt tatggctact gacccagtaa cttcagctcg
     9121 aacagaaaaa gggaaatgga tttatggctt tctgataggc tgtatggcta tcatcattcg
     9181 cgttttgaac ccaggttatg ctgaaggaat gatgctagct attttgctga tgaatatttt
     9241 tgctccatta attgattatt atgtagttga agcaaatgtt aaacaacgca acaaaagaat
     9301 aacaaagcca gttcaataaa tttaaactga aagctctatt tacaattatt aaattaaaga
     9361 taaagaatgg ataccaatag taacagttat actttaattt atgctacaat agtagtggta
     9421 attgtagcat ttatgttagc ttttgtatca ggagcattga aagaaaaaca aaatgcaaat
     9481 attgaacttg acaaaaaaag acaaatttta agttccttac aaatagacac aaaagggaaa
     9541 gatgtagcaa gtttatacga tcaatatatt gtgaaaggtt tggttataaa ctataaaggt
     9601 gaaattcttt cagagtccaa aaaagaagct tttaatattg attttgccaa agaattgaca
     9661 aaaaaattag aagacagacg tttacctctt tatattgcca acgttgatgg agaaacaaaa
     9721 tatatcctta ctttgcgagg gacaggattg tggggaccta tctggggtta tcttgctttg
     9781 aatgatgata aaaatactat ttatggtgtt tatttttctc atgccagtga aacccctggt
     9841 ttaggtgcaa gtattgccga actgcctttt cagaagcaat ttatcggtaa acatatttta
     9901 aatgataaaa atgaatttgt ttctattgct gtaatgaaag ctggtaaaaa agccgaagga
     9961 aaagatcagg tggatgctat ttcggggggt actattacca gtaaaggagt agaagctatg
    10021 ttattgaact gcataggaca atatgttcaa tttttacaac aaccaaccac aggaggaatg
    10081 aatcaatgag taaattattt tcaagaaaat caaaacaagc ctttttatgg ccgctgagca
    10141 agaataatcc tattatggta caaatattag gaatttgttc agctcttgca gtaacagcca
    10201 aactgaaacc agctttggta atgggaattt ctgttactat tatagttgct ttttctaacg
    10261 ttattgtttc attgatacgt gataccattc ctaatcgaat aaggattatt atccaattag
    10321 ttgtcgttgc tgctttggtg accatagtaa gcgaaatttt aaaagcctat gtctatgatg
    10381 taagtgtcca attatctgtt tatgtgggtt tgattattac aaattgcata ttaatgggac
    10441 gattggaagc ttatgcaatg tctaataaac cgtgggattc ttttctggac ggtttaggta
    10501 atggattagg ttatgcaatt atattggtaa ttgtaagctt tttccgtgaa ttgttaggtt
    10561 ctgggacttt gtttgatatt cgtgtgattc ctgcctcatt ttatgaggct ggttatatga
    10621 acaatgggct gatgttaatg cctccaatgg cattgatatt aattgcatgt attgtttgga
    10681 tacatcgtta tcataacaaa gaattacaag aaaaataaaa atttcaaaga taaattttaa
    10741 caatggaaaa tgtatttaat atattcgtta aatcgatttt tgtcgataac atgatttttg
    10801 catatttttt aggaatgtgt tcgtatgtgg ctgtttctaa aaatgtaaaa acttcaatgg
    10861 ggttaggtat agctgtaatt ttcgtcctgc ttatcactgt tcctgtgaat tatcttcttg
    10921 aaaattatgt tttgaaagaa ggatccttga aatggctaag tcctgaatta gcaaatcttg
    10981 atttaagtta tttatctttt attttattta ttgcagtaat tgcaggaata gttcaatttg
    11041 ttgaaatggt ggttgaaaaa tttcttcctt cgctttataa ttcattagga attttcttgc
    11101 ctttaatagc agtgaattgt gctattatgg gtgcttgtct ttttatgcag caacgtgctt
    11161 atcaaaatat tggagaagca acagcttatg ctcttggttc aggaattgga tggtggcttg
    11221 ctattattgg attagctgct ataagagaaa aaatggaata ctccgatgtt cctgcaccgc
    11281 ttaaaggact tggtattaca ttcattaccg ttggattaat gggaatagca tttatgtgct
    11341 tttctggatt aaaactctga cctttacttg tttaaaatca ttaaaaaatt ctactttaaa
    11401 ataaataagc ttatatttat atgttattag cgatagatac agtcaatttg gttgccagtt
    11461 tagtagtttt tcttgttgta attttacttt tagttattat tttgcttgtt gcaaaacaat
    11521 atttaatttc ttctggtacc gtaaccatca ctattaatga gaataaaaaa ttagaagtgc
    11581 cagcaggagg aacgttatta aatacattag cgatgcaaca aatttttctt ccttccgctt
    11641 gtggaggtaa aggcacttgt gctcaatgta agtgtcaagt tttcgaaggt ggtggtgaaa
    11701 ttttacccac agaaattagt cattttactc gaaaagaaat taaagaaaaa tggagattaa
    11761 gttgtcaggt aaaagtaaaa gaaaatattt ctattaaaat accaccttct attttaagtg
    11821 tcaaaaaatg ggagtgtaaa gtaatttcca acaaaaatgt agctactttt atcaaagaat
    11881 tgattgtaaa attaccagaa ggagaaagat tagattttat tcctggaagt tatattcaaa
    11941 tagatattcc taaatataat acgattaaat tttctgattt tgatatagat gaacgtttcc
    12001 gttccgattg ggataaaatg aagctttggg atttatcgtg tgataatgat gaagaaacaa
    12061 tgcgagctta ttctatggca aattatccag cagaaggaaa agatattgta atgttaaatg
    12121 tacgtattac tactccacca attgataaat ctacaggcac atttatggat gtaaagccag
    12181 gcatcgcttc aacgtatatt tttaacctca agccaggtga tagagtaaaa gtatcgggcc
    12241 catttggaga attccatccc attatcaatt ctaaacgcga aatgctttat attggaggag
    12301 gtgctggaat ggctcctctg cgttctcata ttttacattt acttaaaaca ttgcagatac
    12361 gcgatagaaa aat
//
LOCUS       DAOTKS010000006        11508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_6002_length_11508_cov_3.801013, whole genome shotgun sequence.
ACCESSION   DAOTKS010000006 DAOTKS010000000
VERSION     DAOTKS010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11508
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6002_length_11508_cov_3.801013"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..904)
                     /locus_tag="P5236_00285"
     CDS             complement(131..904)
                     /locus_tag="P5236_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013815398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRU62837.1"
                     /translation="MGKIISLANQKGGVGKTTTAINLAASLATLGEKVLLVDADPQAN
                     ASSGLGINVQSLQFTIYECLIDGVEPKDAILPTEIENLFILPSHIDLVGAEIEMLNLD
                     HREKVMQNLLRPLKPYYNYILIDCSPSLGLITVNSLTASDSVIIPVQCEYFALEGISK
                     LLNTIKIIKSKLNPSLEIEGFLLTMYDNRLRLANQVYAEVKKHFQEMVFDTVIARNVR
                     LSEAPSYGKPVMVYDPESKGAINYQDLAKELIAKQKVKL"
     gene            1333..>2235
                     /locus_tag="P5236_00290"
     CDS             1333..>2235
                     /locus_tag="P5236_00290"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HRU62838.1"
                     /translation="MSYKNQKNKKEISILIFLFILVFILILIFPFRLDLSSDKRYTIS
                     PVSKKLMQQLDAPLKVTLYLEGDLNPGFLRMKKSTIQMLEELSAYSKKGIEIKTINPS
                     LAKSSTEREKNYLKLSSHGLTPTAVYERDKEGKAIQKIIFPWLEIEYKGNKTLVNLLK
                     NLPGNSGEQNINISIENLEFEITDAIRRLTSTQVNKIAFLEGHGELSEAETYDISKAL
                     SRYFQIDRGIIGTDASILDDYKVLIVAKPMFPFSEKDKFIIDQYIMKGGRILWLIDGV
                     QLSKENLSTVGFSPAWALDINLNDQ"
     assembly_gap    2236..2335
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2336..3182
                     /locus_tag="P5236_00295"
     CDS             <2336..3182
                     /locus_tag="P5236_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HRU62839.1"
                     /translation="STVGFSPAWALDINLNDQLFRYGVRVNPVLLQDVQCVLVPVNVA
                     SQNQSPHFEPMPWYFAPLLLTSYQHPITRNIAEVKTDFCSSIDAVGNNPHIKSTVLLA
                     TSNNTHIIPTPATIDLRVLPKANDKEYFNLGYVPVAILVEGSFESVFRNRMLPAGLIN
                     INKVQYQSKPTRQIFVADGDIIRNETSGLATDSTTLSLGFDRYMNQQFGNKDFIQNAV
                     LYLADEEGWMQLRTKTFKLRLLNKNAVRDKKNICQVINISVPIILLALFGIIYQWIRK
                     RKYSK"
     gene            3602..4285
                     /locus_tag="P5236_00300"
     CDS             3602..4285
                     /locus_tag="P5236_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRU62840.1"
                     /translation="MKDIRESILLEASFLFHQLGIKNISIDEICSNLRISKKTFYSYF
                     DQKQDLVDAVLFYQEEFYLKKLEGILKNKNAIEFLILSLKEIKKRANCEPTLMWNDIK
                     KYYPEVFKKHILIRQDFLKKLFETNLKRGVEEGFYRKDLDIILISILYSEHLKNIFNT
                     LNKYSKKYSKKRLIDFFIDMIIHVIANEEGLQYVQENYKGVEENPVEMKKNENILNNY
                     RSESKGVFN"
     gene            4322..5659
                     /locus_tag="P5236_00305"
     CDS             4322..5659
                     /locus_tag="P5236_00305"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRU62841.1"
                     /translation="MNKKHLKRIFFVVISLLLINNKLYAQDTLIVTLSDAIEIALSES
                     PTIKIADKEIERVDYSKKEKRGALFPSINAVGNYSRAIKKQKLFFSLPGMPPRPEGVE
                     VGQDNTFSGGISASLPIIAPTLWATIQMSELDAELALESARSSKLSLINEVSKSYYAT
                     LLAQDSYDVFKKSYDIAIENAKVIENKYKQGVVSEFEWIRADVQVKSASTNLVSAENA
                     VTLSILKLKMLMGIDMFTGLKVKGNLADYEATKYSDVLAIDTTTLQANSDLKQFDIKT
                     QQLQQTLKIQKSTLMPTLSASFNYQYMTMANDSVAFSNYHWFPTSTGAITLAIPLFQG
                     GEKINKQKQIKVQLDEMKYQRDNLRRGLELQAMSYLDNINQAIKTIETNKEGLRQAEK
                     ALLISQKMYEVGASTYLDLSNAELVYTQAGLAYNQSIYNYLSAKADLEKLLGK"
     gene            5711..6730
                     /locus_tag="P5236_00310"
     CDS             5711..6730
                     /locus_tag="P5236_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRU62842.1"
                     /translation="MKRTIEFGILFLAIVFGFTSCGKSGQKKTKIESEIPNVRIQQVS
                     LRDVERYANLTATILPEVKNNVAPAAPGRIRKIFVEVGDHVSKGQKLVQMDDANLSNL
                     ENQVANYQRIYNRVLELFNVGGASQQELDNAKLQLDVATTNLQNLAENTYLLSPTNGV
                     VTARNYDNGDIYSAQFPVLVVMQINPVRAIVNVSETNYSDVKLGMPAQVTFDILKNEF
                     FTGKVNLIYPTIDERTRTFSVEIKLNNNDYKIRPGMFARVKLNFGTVKSIVVPDQAVI
                     KQSGSGEYFVYIYKDGKVTYQKVELGQKIGNEYEIISGIEDGAQVVVAGQARLADGVK
                     VNVVK"
     gene            6793..9903
                     /locus_tag="P5236_00315"
     CDS             6793..9903
                     /locus_tag="P5236_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007480126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRU62843.1"
                     /translation="MSLYESSVKRPIMTSLIFIGVVILGLFSLSKLPVDLLPNVEPNI
                     LMVVTNYSGASALDIETNVTRPLENVLNTVENLKNITSNSKENTSIIIMEFEFGQDLD
                     VVSNDVRDKLELVKPYLPEGTTNPVIFKFSTDMMPIVIISATADKSIPALYKILDDNV
                     ANPLARIKGVGAVSISGAPQREIQVYIDPVKLEAYHLTIEAVGQKIRLENINTPSGSM
                     DIGNKTYSLRVQGEFKDPSELRKVVVGTYGNKTIYLSDIAIISDSHEERVQESYTNGV
                     QGATIKIQKQTGANTVAIVNAIREKLPEIQKTLPSDIKLNVIVDSSDNIKKTIAGLEE
                     TIMYAFIFVLLVVLFFLGRWRATIIIILTIPISLIAAFIYLAATGGTLNIISLSSLSI
                     AIGMVVDDAIVVLENITKHIEKGSRPTSAAIHGTDEVATAVVASTLTIVAVFFPFTLV
                     SGLAGILFDQLGWMVTLIMIVSLVCAMALTPMLSSQLLTVEKGHGKFFTKVYSPIERF
                     LNNLDNWYAGLINWAVRHRWQMVLIAAGFFILSLIPLIGIGTEFIPPQDNARISATVQ
                     LPIGTRMEISKETAEELTKLWREKYPEIETINYTVGPASSDNVLGWVMTTGTHLISYT
                     FQLVDANERERSIFEIGDSIRHDLNIRPEIAKSTVTAGGAMGRIGGQSIIDVEIYGYD
                     FNQTDKVAKELTNRIDKNIKGLVNTTISREDYSPEYQVIFDREKLALNGLDIATASTF
                     LRNRINGLVASLYREEGDEYNIRVVYAPEYRETVEDIENILIYNSQGQAIRLKDLGNV
                     VETLTPPYIQRKNRERVITVSSTVSGTTIDKAVRGLRTQLSQMDIPTGIDAEITGTYE
                     DQQESFADLGTLLIIVILLVYIVTASQFESLTYPFIIMFALPFAFSGVVLALALSGNT
                     LNMMSMIGIVMLVGIVVKNGIVLVDYINLNRERGMGIIRAIVSGGHSRLRPVLMTTLT
                     SILGMVPLAISNSQGSEIWKPMAYTIIGGLTVSTILTLVIVPTLYAIFAGFGINRKYH
                     KLQKQH"
     gene            10061..10357
                     /locus_tag="P5236_00320"
     CDS             10061..10357
                     /locus_tag="P5236_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU62844.1"
                     /translation="MKSIFIVFDQGFYQQVLEILYINHHVRGFTSWERVLGRGSKTGE
                     PHYGTHAWPSLNGAILTVVPDEKVKPILEDLKALDATSDVMGIRAFVWNIEDGM"
     gene            complement(10447..11400)
                     /gene="tal"
                     /locus_tag="P5236_00325"
     CDS             complement(10447..11400)
                     /gene="tal"
                     /locus_tag="P5236_00325"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HRU62845.1"
                     /translation="MNLLEQLKTYTKVVADTGDFESIINYKPLDATTNPSLIFAASQQ
                     EKYQHLILDAIQYAKKSHVDKSQQLKLALDKLSVNFGIKILEIVPGRVSTEVDARLSF
                     DTGGTIERARDLIALYEKEGISRERILIKIASTWEGIEAAKILEKEGIHCNLTLLFSL
                     VQAIACAEARVTLISPFVGRILDWYKKEGFSFNSPSEDPGVKSVVSIYNYYKKFDYLT
                     QIMGASFRNIQQIIELAGCDLLTISPTLLKELEQTEGILERKLDPEKAKSNDVQRITI
                     DEKIFRWMLNEDAMATEKLAEGIRNFTKDLRKLENKIAELL"
BASE COUNT         3920 a         1627 c         1971 g         3890 t
ORIGIN      
        1 gacgatgaac cgcttgtata aatattttca gtgtcaatta aggcatccag acctcttcct
       61 aatactgatt tttttaccat aacttttttc tgtggaacga tttttttatc tctttattat
      121 tttaaactga ttaaagtttt actttttgct tggcaatcaa ttctttagct aaatcttgat
      181 aattaatagc tccttttgat tctggatcat ataccatgac tggcttaccg taactggggg
      241 cttcacttaa acgaacattg cgagcaataa cggtatcaaa aaccatttcc tggaaatgtt
      301 tttttacttc agcatatact tgattggcta aacgtagtct gttatcatac atcgtaagta
      361 aaaaaccttc aatttccaga gaaggattca atttgctttt tatgatttta atggtattta
      421 aaagtttact aattccttca agggcaaaat attcacattg aacaggaatg attacagaat
      481 cagaagcagt aagtgaattt actgtgataa gccctaaaga aggagaacaa tcaatcaaaa
      541 tataattgta ataaggtttt aaaggtcgaa gcaaattttg cattactttt tctcgatggt
      601 ctaaattcaa catttcaatt tcagccccaa ctaaatcaat atgagaagga agaataaaca
      661 gattttcaat ttctgttggc aaaatggcat ctttaggttc aactccatct attaagcatt
      721 cataaatggt aaattgcaat gattgaacat tgatacctaa ccctgaagaa gcatttgctt
      781 gcggatcagc atctactaat aaaacttttt caccaagggt tgctaatgaa gcagctaaat
      841 taatagctgt tgttgtttta ccaactcctc ctttttgatt ggctaatgat attatttttc
      901 ccataaatta ttaataatca atttatcaat aacttaataa aattgacagg tattttttca
      961 accatcaaga tttttcagat ttatataaaa tatgatatgg acaaagataa taaaataaat
     1021 ttaccagttc aataaattag ttgttcagtg gaatagaaat aagatttttt attaatctgt
     1081 aacaagtcaa aatatttact ttaaagactt attttttatg tttatagttt taaattttta
     1141 ccgtaaaata gtatgaaatt tgcaaacaat attcttaaat ttaagttatt tgcatacgca
     1201 gattcacaag atgaaattta tacaaataag catattttac ttttactctt tcttgaattg
     1261 attatctttg caagaaaaaa tttctttaaa gcactgaaaa aaatcatagt taattaaatc
     1321 ttctcttttt caatgagtta caaaaatcag aaaaataaaa aagaaatttc tattttaatt
     1381 tttttattca ttcttgtctt tattttaatt ttaatttttc cattcagatt agatctttct
     1441 tctgacaaac gatataccat ttcgccagtt tcaaaaaagt taatgcaaca acttgacgct
     1501 ccactcaaag taactcttta tcttgaagga gatttaaatc ctggtttctt gaggatgaaa
     1561 aaatcaacga ttcaaatgct ggaggaatta tctgcctatt caaaaaaagg aatagaaata
     1621 aagactataa atccttcact ggcaaaaagt tcaacagaaa gagaaaaaaa ttatttaaaa
     1681 ttgagttcgc atggcttaac tccaacagca gtttatgagc gtgataaaga aggaaaagcc
     1741 attcaaaaaa ttatttttcc ctggcttgaa attgaatata aggggaataa aacattggta
     1801 aatttattga agaatttacc aggcaattct ggtgagcaga acatcaacat ttccatagaa
     1861 aaccttgaat ttgaaattac agatgctatt cgccgattga caagtaccca agtaaataaa
     1921 attgcttttc tcgaaggtca tggcgaactt tctgaagcag aaacttatga tatcagtaaa
     1981 gctttaagcc gatattttca aattgatcga ggtataattg gcacagatgc ttcgatatta
     2041 gatgattata aagttttgat tgttgcaaaa ccaatgtttc ctttcagtga aaaagataaa
     2101 ttcattatag atcaatacat tatgaaagga ggtcgtattc tttggttgat tgacggggtg
     2161 caactttcaa aagagaatct ttcaactgtt ggattttcgc ctgcatgggc attggatata
     2221 aatttaaacg atcaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttcaa
     2341 ctgttggatt ttcgcctgca tgggcattgg atataaattt aaacgatcaa ctatttagat
     2401 atggtgttcg tgtcaatcct gttctattgc aagatgtaca atgtgtctta gtgcctgtaa
     2461 atgttgcttc tcaaaaccaa tctcctcact tcgaaccaat gccttggtat tttgcacctt
     2521 tgcttcttac ctcctatcaa catcctatta ccagaaatat agcagaagtt aaaacagatt
     2581 tttgttccag tattgacgca gtaggcaaca atccacatat aaaatcaacg gtattgcttg
     2641 ctacttctaa taatactcat attattccta cacctgctac tattgattta agggtattac
     2701 caaaagccaa tgataaggaa tattttaatt tagggtacgt gccagtagct attctggtgg
     2761 aaggaagttt tgaatcggtt tttaggaaca gaatgcttcc tgcgggtttg ataaatataa
     2821 ataaggttca atatcaaagc aaaccgactc gtcagatttt tgtggcagat ggggacatca
     2881 ttagaaacga aacttctggg ttagcaaccg atagtacaac attatcctta ggttttgaca
     2941 gatatatgaa tcaacaattt ggtaataaag attttattca aaacgcagtg ttatatttag
     3001 ccgatgagga aggttggatg caattacgta ccaaaacttt taagttgcgt ttactaaata
     3061 aaaatgctgt acgtgataaa aaaaatattt gtcaagtaat caatatttca gttccaataa
     3121 ttttattagc tctttttgga attatctatc agtggataag gaaaagaaaa tatagcaaat
     3181 aaaggggaaa aagaaaaagg aaaccataat aaaataaaga ttgaatgccg taagtttgaa
     3241 aaaggtgcct ttgaattttt taccactatt catagcaaca atcgttaata ataaaaaatt
     3301 tgtaactccc tccattatag aggttattat tattaggtga ttgattttta aataatttcc
     3361 tttattggaa ctcttttttg gtaatttttt attcgaacca ccaatatttt tatttttttc
     3421 attttattgt ttttttaaaa aaccaggaaa cttttttatt tttgaagttt tttaattaac
     3481 tttttatttg attgattatc aattatttat aaatcattca gtaattatta actattaata
     3541 aatgattttt tgatttttgg aataagtttt tttctaattt tgccaaactt tctaattata
     3601 catgaaagac atacgagagt ccatattact tgaagcaagt tttctatttc atcaattagg
     3661 tataaaaaat atatctattg atgagatatg tagtaatttg cgtatttcaa aaaaaacctt
     3721 ttattcttac tttgatcaaa agcaagattt ggtagatgct gtgcttttct atcaagaaga
     3781 attttatttg aaaaagctcg agggtattct aaaaaataaa aatgctattg agtttctaat
     3841 tttatcattg aaagaaatca aaaaaagagc aaattgtgag ccaacactca tgtggaatga
     3901 tataaaaaaa tattatcctg aagtttttaa aaagcatatt ttgataagac aagattttct
     3961 gaaaaaactt tttgaaacaa atttaaaacg tggtgttgaa gaaggttttt atagaaaaga
     4021 tttagatata attttgattt cgattcttta ttccgaacac ttaaaaaata tttttaatac
     4081 cttgaataaa tattcaaaaa aatattcaaa gaaaagattg atagactttt ttattgatat
     4141 gattattcat gtaattgcta atgaggaagg attacaatat gttcaggaga attataaagg
     4201 tgtggaagag aatccagtag aaatgaaaaa gaatgaaaat attttaaata attatagatc
     4261 agaatcaaag ggagttttta attaaatttt agttgaattg aataatttaa aagtataaaa
     4321 tatgaataaa aaacatttga aaagaatttt ttttgttgtg ataagtttat tattgattaa
     4381 taataagtta tatgctcaag atacacttat cgtaacttta tcagacgcca ttgaaattgc
     4441 cctgagcgaa agccctacta ttaaaatagc tgataaggaa attgaacggg tagattattc
     4501 aaagaaagaa aaacggggtg ctttatttcc ttccataaat gctgtgggga attattcgag
     4561 agcaataaaa aaacagaagt tgtttttctc tttacctggt atgccaccac gacctgaagg
     4621 agtagaagtg ggacaagaca atacctttag tggcggtatt tcagcttctc tccctattat
     4681 tgctcctact ttgtgggcaa ccattcaaat gtcagagctg gatgctgaat tagcactcga
     4741 atccgctcgt tcttcaaaac tttctttaat taatgaagtg tcaaagtctt attatgctac
     4801 tttattagct caggattctt atgatgtgtt caaaaaaagt tatgatattg ctattgaaaa
     4861 tgcaaaagtt atagaaaaca agtataaaca aggagtagta tctgaatttg aatggataag
     4921 agcagatgta caggtgaaaa gtgcaagtac taacctcgtt tcagcagaaa atgccgtcac
     4981 tctcagtatt ttgaaattaa aaatgcttat gggtattgac atgtttacag gactgaaagt
     5041 aaaagggaat ttagcagatt atgaagctac aaaatattcg gatgtattgg ctatcgatac
     5101 tactaccttg caagccaact ctgatttaaa gcaattcgat attaaaaccc aacaacttca
     5161 acaaacttta aaaattcaga aaagcacttt aatgccaact ctttcagctt cttttaatta
     5221 tcaatacatg acaatggcaa atgattctgt ggctttttca aattatcatt ggttcccaac
     5281 ctctacagga gcaattacac ttgccattcc tctttttcaa ggtggggaaa aaataaataa
     5341 acaaaagcaa ataaaagttc aattggatga aatgaaatat caacgcgata acttaaggcg
     5401 aggcttggaa ttacaagcaa tgtcgtattt agataatatc aatcaagcaa ttaagacaat
     5461 tgaaaccaat aaagaaggat tacgtcaggc tgaaaaggca ttgcttatat cgcaaaaaat
     5521 gtatgaagtg ggagcttcta cttatcttga tttgtcaaat gcagaattag tctatacgca
     5581 agctggttta gcttataatc agtctattta taattatttg tcagcaaaag ctgatttaga
     5641 aaaactttta ggaaaataaa atataaagaa ttaattagat ttttaaacaa ttaaataatc
     5701 aatattcaaa atgaaaagga caatagaatt tggtattctg tttttagcaa tagtatttgg
     5761 atttacttca tgcggaaaat caggacaaaa gaaaaccaaa attgaaagtg aaattccaaa
     5821 tgttcgtatt cagcaggtgt cattgcggga tgttgaacga tatgccaact taacagcaac
     5881 tatcttgcct gaagtcaaaa ataatgttgc accggctgca ccaggtcgaa tccgaaaaat
     5941 attcgtcgaa gtaggtgatc atgtttcaaa agggcaaaaa ttagttcaga tggatgatgc
     6001 aaatttatca aatttagaaa atcaagttgc taattatcag cggatttata atcgtgtttt
     6061 agaacttttt aatgtgggtg gtgcttctca gcaggaattg gataatgcaa aattgcaact
     6121 tgatgtagca acgaccaatt tgcaaaattt ggccgaaaac acttatttgt taagtcctac
     6181 caacggagtt gttactgctc gaaattatga taatggagat atttatagtg ctcaatttcc
     6241 tgtgttagtt gttatgcaga taaatccggt aagggcaatt gtaaatgttt ctgaaacaaa
     6301 ttattcagat gtaaaattag gaatgcctgc tcaagtaaca ttcgatattt taaaaaatga
     6361 attttttact gggaaagtga acttaattta tcctactatt gatgaaagaa ccagaacttt
     6421 ttctgttgaa atcaaattaa ataacaatga ttacaaaatt cgtcctggta tgtttgcaag
     6481 ggtaaaatta aattttggca cagtaaaatc tattgtagtt cctgatcagg cagtaattaa
     6541 gcaaagcggt tcaggtgaat atttcgttta tatttataaa gatgggaaag tgacctatca
     6601 aaaggtagaa ttagggcaga aaattggaaa tgaatatgaa attatttccg gtatagaaga
     6661 tggggcacaa gtagttgttg caggacaagc tcgccttgct gatggagtaa aagtaaatgt
     6721 agtcaaataa tgattattga aaaaataaac tgagcaaagt tttttaaata acgaagttct
     6781 aaatttaaaa ttatgagttt atacgaaagt tctgttaaaa gacctattat gacttctctg
     6841 atttttattg gggtagttat tttaggattg ttttcattga gtaaattacc tgttgattta
     6901 ttgccaaatg tagaaccaaa tatcttaatg gtagttacta attattctgg agcgagtgct
     6961 ttagatattg aaacaaatgt aactcgtcct ttggaaaacg tattaaatac agttgaaaat
     7021 ttaaaaaata taacttccaa ttcaaaggaa aatacttcca ttattattat ggaatttgaa
     7081 tttggtcaag atttggatgt agtttccaat gatgtgcgtg ataaattgga attagtaaaa
     7141 ccttatttac ctgaaggaac taccaatcca gtgattttta aatttagtac cgatatgatg
     7201 cctattgtta ttatatcggc tactgcagat aagagtattc cagctcttta taaaattttg
     7261 gatgataatg tagctaatcc cttagctcgt atcaaaggag taggtgctgt gtctatttca
     7321 ggtgctcctc aacgagaaat acaagtgtat attgacccgg taaaattgga agcttatcat
     7381 ttaacgattg aagctgttgg tcaaaaaatc cgattagaaa acattaatac accaagtgga
     7441 tcaatggata ttgggaataa gacttattct ttaagagtgc agggtgagtt taaagatcct
     7501 tctgaattga gaaaagttgt agtaggaact tatggcaaca aaaccattta tttgtccgac
     7561 atagctatca ttagtgatag tcatgaagaa cgagttcaag aaagctatac caatggtgta
     7621 caaggtgcaa ctataaaaat tcaaaaacaa acaggagcta atactgtagc tattgtaaat
     7681 gctatccgtg aaaaattgcc tgaaattcaa aaaactcttc ccagtgatat taaactaaac
     7741 gtcattgtag acagctccga taatattaaa aagactattg ctggattgga agaaaccatt
     7801 atgtatgctt ttatatttgt attgttagtg gttttattct ttttaggaag atggagagct
     7861 acgattatca ttattttgac aattccaatt tcattgatag cagcatttat ttatttagca
     7921 gcaacaggtg gaaccttgaa tattatttct ttaagttctc tttccattgc cattggaatg
     7981 gtagtagatg atgcgattgt ggtacttgag aatattacta aacatattga aaaaggaagt
     8041 agacctacaa gtgctgctat tcatggcaca gacgaagttg ctactgctgt ggttgcttca
     8101 accttaacca ttgtggcggt attttttccc ttcaccttag tatcaggttt agcaggaatt
     8161 cttttcgacc aattaggatg gatggtaacc ctcattatga ttgtttctct ggtttgtgcc
     8221 atggctttaa cacctatgct aagttcccaa ttgttaactg tagaaaaagg tcatggtaaa
     8281 tttttcacta aagtttattc tcctatcgag cgtttcttaa ataatttgga taattggtat
     8341 gcaggattga ttaattgggc ggtacgtcac agatggcaaa tggtattaat tgctgctggt
     8401 ttctttatct taagcttgat tcctttgata ggcattggaa cggaatttat tccaccacaa
     8461 gacaatgctc gtatttcagc tacagtgcaa ttgcctattg gaacaagaat ggaaatttca
     8521 aaagaaaccg cagaagaatt gactaaactt tggagagaaa aatatccaga aattgaaact
     8581 attaattata ctgttggacc agcaagtagc gataacgtgt tgggctgggt tatgacaaca
     8641 gggacacatc ttatttctta tacttttcag ttggtggatg ccaatgaacg agaaagaagt
     8701 attttcgaaa taggtgatag catccgtcat gatttaaata tacggcctga aattgcaaaa
     8761 tcaactgtta ctgcgggagg tgcaatggga agaattggag ggcaatccat tattgacgtg
     8821 gagatctatg gttatgattt taatcaaaca gataaagtag caaaagaatt gactaatcgg
     8881 atcgataaga atataaaagg cttggtaaat accacgatta gtcgtgaaga ttattccccg
     8941 gaatatcaag tcatattcga tagagaaaaa ttagctttga atgggctgga tattgctacg
     9001 gcttcaactt ttttgagaaa tagaattaat ggattagtag caagccttta tcgtgaggaa
     9061 ggagatgaat acaatatccg ggtagtttat gctcctgaat atcgtgaaac tgttgaagat
     9121 atagagaata ttttaattta taatagtcaa ggacaagcaa ttcgtttgaa agatttgggt
     9181 aatgtggtag aaacattaac tccaccttat attcaaagga aaaacagaga gcgtgttata
     9241 actgtttcaa gtaccgtttc aggaacaaca attgataaag cagtgagagg tttaaggact
     9301 caattatccc aaatggatat tcctacggga atagatgctg aaattactgg aacttatgaa
     9361 gatcagcaag aatcattcgc tgatttgggt actttgttga ttattgtaat tttactggta
     9421 tatattgtga cggcatctca atttgaatct cttacttatc cttttattat tatgtttgcg
     9481 ttgccatttg ctttttcagg agtagtttta gctttagcat taagcgggaa tacacttaat
     9541 atgatgtcaa tgataggtat tgttatgttg gtaggaattg tggttaaaaa tggcattgtg
     9601 ctggtggatt acataaattt gaatagagaa cgggggatgg gtattattcg agccattgta
     9661 agtggaggtc attctcgttt gcgtcctgtt ttaatgacta ctttaacctc tattttagga
     9721 atggttccgt tggctattag taattctcaa ggttcagaaa tttggaaacc aatggcttat
     9781 accattatag gaggtttaac agtatctact attctgacat tggtgatagt gccgacattg
     9841 tatgctattt ttgcagggtt tggtatcaat cggaaatatc ataaattgca aaaacaacat
     9901 taacggagaa attacatttg atctttttta ctctttattt atgattttat gaggagataa
     9961 aagatcaagt attgtgaaac tttaaaattc aacaagagta aaaaaatagc cagttttaaa
    10021 agcaatgttt ttactcaaac ttatttaaaa aaattatttc atgaaatcaa tttttattgt
    10081 ttttgatcaa ggtttttatc aacaagttct cgaaatactt tatatcaatc atcatgttcg
    10141 tggttttaca agctgggaaa gggttttggg acgtggtagc aaaacaggtg agcctcatta
    10201 tggcactcat gcatggcctt cattaaatgg ggctatccta accgttgttc cagatgaaaa
    10261 agtaaaaccc attttagaag atttgaaagc attggatgcc acttctgatg taatgggcat
    10321 acgtgctttt gtatggaata ttgaagatgg aatgtaaaat tttagacttt aaaaaggcac
    10381 tttgaatagt tattataaaa agtgcctttt taatatgttt tatgatgtta ccacctatga
    10441 attttctcaa agtaattcgg caattttatt ctccaatttt cttaaatctt ttgtaaaatt
    10501 acgtattcct tctgctaatt tttccgtagc catagcatct tcatttaaca tccatctaaa
    10561 aattttttcg tcaattgtaa tacgttgaac atcattgctt tttgcttttt ctggatcaag
    10621 ttttctctct aatattcctt ccgtttgttc cagctctttc aataaagtgg gagagatagt
    10681 gagtaagtcg catcctgcaa gttcaataat ttgttgaata tttctaaaac tggctcccat
    10741 tatttgggta agataatcaa attttttgta ataattataa attgacacaa ctgattttac
    10801 tccggggtct tcgcttggag aattaaatga aaaaccttct tttttgtacc agtctaaaat
    10861 tcgtcctaca aatggtgata tgagagtgac tcgtgcttct gcacatgcta ttgcttgtac
    10921 caatgaaaaa agtaaggtta aattacaatg aatgccttct ttttctaata ttttggctgc
    10981 ttcaattcct tcccatgtcg atgcaatttt tattaatatt ctttcccgtg aaataccttc
    11041 tttttcatat aaggcgatta aatcccgagc tctctcaatc gttccccctg tatcaaaaga
    11101 taaacgtgca tcaacttcgg ttgaaactcg tcctggaaca atttccagaa tttttattcc
    11161 aaaattaacc gataatttat ctaatgccag ctttaattgt tgtgatttat ctacatgaga
    11221 tttttttgca tattgaatgg catcaagtat taaatgttga tatttttcct gttgagaagc
    11281 agcaaaaatc agcgagggat ttgtagtagc atccaatggt ttataattaa taatagattc
    11341 aaaatcgcct gtatcagcaa ctacttttgt ataagttttc aattgttcta agagattcat
    11401 aaccgtaaat tcaagtaatt aatgcaactt tttcagggaa ataaaaggta aagaaaaatt
    11461 atttttcaag acaaaggaga aaaaagagca tttccccttt gccctgat
//
LOCUS       DAOTKS010000007         9854 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_7150_length_9854_cov_4.044903, whole genome shotgun sequence.
ACCESSION   DAOTKS010000007 DAOTKS010000000
VERSION     DAOTKS010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9854
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7150_length_9854_cov_4.044903"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="P5236_00330"
     CDS             complement(<1..542)
                     /locus_tag="P5236_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HRU62846.1"
                     /translation="MKRIFFSLILLFSVQISFANLLYDIVDGKFKVKDLVVPYSMNDG
                     ENYTLMVNKKAIVKFNYKTGGVVDTLFSVSRLQNAPFKEIEGYEFSPNEAKLLVRTNV
                     KKRYRRTFIADYYLYDIKRNEIQPLSEYGPQESPLFSPDSRYVAFARNNNLYLKKLDF
                     NTESAITKDGEKNKIINGVP"
     gene            757..1578
                     /locus_tag="P5236_00335"
     CDS             757..1578
                     /locus_tag="P5236_00335"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRU62847.1"
                     /translation="MGKKLFENNSSTKAVGICLSGGGALGFAHIGVLQSLEEHGIYPL
                     QVAGSSMGAVVGVLYAAGISPLKMLQIIKEDKLYRITKLMNIRLTSINSGLSTHDTLR
                     EIIRELIPHNSFDKLPKKMNVCVSNLNTADWEIVNTGENLDKWVAASASIPGIYEAMQ
                     MNKCFYVDGGLLNNFPAQALEHSCQKIIGVDVIPHFETLQLKKPTDTFSWSVRIMEHK
                     NSLPGRKLCHFLIEPMAIKKYHGFSFDAYQKIYEEGYKSTNNYIEKNPEILNLKN"
     gene            complement(1663..2259)
                     /gene="folE"
                     /locus_tag="P5236_00340"
     CDS             complement(1663..2259)
                     /gene="folE"
                     /locus_tag="P5236_00340"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HRU62848.1"
                     /translation="MDSELNGGKIFDEKKTNEIAQHYKNIISLLGENPERPGLVQTPE
                     RVARAMQFFLQGYEEDPEEIIREAMFTENYRQMVIVKDIDFYSLCEHHLLPFFGRAHV
                     AYIPNKKITGLSKIARVVEIYARRLQVQERMTTQIKQCIQNTLQPMGVMVIIEAQHLC
                     MQMRGVQKLNSITTTSDFTGVFQEARTREEFMNLIKNR"
     gene            complement(2278..2802)
                     /locus_tag="P5236_00345"
     CDS             complement(2278..2802)
                     /locus_tag="P5236_00345"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRU62849.1"
                     /translation="MVVIKETFCNFATIYHFNKMHQLFQIFIVTLLFSLPIFSLSGQQ
                     KENIFEVLSKNDSTEGTVVFHQDKRIETLLTEKRIPTIPAKTTVVNGYRVQVFSSNEP
                     RTAKNEAFNIERLINETFPEYGVYVTYSSPFWKVRVGDFASMEEAQAFKNELIEVFPR
                     LRNQTYVVRDQINK"
     gene            2908..3639
                     /locus_tag="P5236_00350"
     CDS             2908..3639
                     /locus_tag="P5236_00350"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008153939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HRU62850.1"
                     /translation="MSRIALIVAGGKGERMKTSIPKQFIELQGKPVLMHTMNVFFRYD
                     PALSFFVVLPSEQIDLWKLLCQQHHFDMPHQIVNGGETRFHSVKKGIEAISHQLQKNK
                     KITNKKNVILVAIHDGVRPFVSTDTIDRCFEEAEKEGNAIPAVELTDSIRKVSLLGNE
                     AEERRNYRLIQTPQVFEFSLLQKAYQQQYSPFFTDDASVVEAMGVNIHLVEGNCENIK
                     ITTSFDLELAEFFLSRNESSNRQIF"
     gene            3645..5741
                     /gene="recG"
                     /locus_tag="P5236_00355"
     CDS             3645..5741
                     /gene="recG"
                     /locus_tag="P5236_00355"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRU62851.1"
                     /translation="MDFFNQDIQYVKGVGPKRAELLNKELNIFTVEDLLRHYPYKYVD
                     RSRFYFLNEISENMPFIQLKGKILSFEKAGEGRKQRLIATFTDGFGIMELIWFNGLRF
                     VQDKYKTNIEYVVFGKPTFFNGKISIIHPEIDLPSKLPPPEQMGMQPFYNTTEKMKSH
                     SLNSKAIQKIIYSLLPTLQNSIPETLPSYLLQRFKLLNLTEAIRNIHFPQNSHLLNKA
                     RQRLKFEELFYIQLSIVRQQKWREQKFNGFIFKHIGYFFNTFYHKYLPFSLTNAQKRV
                     IHEIRKDVGSGKQMNRLLQGDVGSGKTLVALMAMLMAADNDFQACMMVPTEILAQQHY
                     TSIKELLGDLGVEVSLLTGSTRQSERKVIHQKLQNGEIHILIGTHALIEETVQFKNLG
                     LVVIDEQHRFGVAQRAKLWTKNQQPPHILVMTATPIPRTLAMTLYGDLNISSIDELPP
                     GRKPIQTFHFYENKRAQLNKFIRQQVEAGRQIYVVYPLIQESEVLDLKNLEEGYCQLC
                     ETFPQYKISMMHGKMKSDEKELQMNKFVSGETQIMVSTTVIEVGVNVPNASVMIIENA
                     ERFGLSQLHQLRGRVGRGAEQSYCILLTNYKLSATTRKRIEVMVRTNDGFEIAETDLQ
                     LRGPGDLEGTQQSGLPFELKIANLAQDGKMLEIARQTALEILEVDPNLEKPENKVLLK
                     QLKKMKNSPINWGIIS"
     gene            5830..6291
                     /gene="ndk"
                     /locus_tag="P5236_00360"
     CDS             5830..6291
                     /gene="ndk"
                     /locus_tag="P5236_00360"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRU62852.1"
                     /translation="MEKTLVIIKPGAIQRELIGEVITRLERKGLHLCGIKMMQLTDDI
                     VSEHYAHLADLPFFDRIKNSMMVTPIIVCCWEGVEAVKVVRAIVGVTNGRDAQAGTIR
                     GDFSMSIQENIIHASDSVETAKIEIARFFKNDEIFDFKQNNLPFIYANDEK"
     gene            6302..7192
                     /locus_tag="P5236_00365"
     CDS             6302..7192
                     /locus_tag="P5236_00365"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRU62853.1"
                     /translation="MNVLTDSPNIYLVLLILFLLLVSGKTKSQSFDMALSAVGSLKDT
                     TNIYKQMFNDDSDDLMENHPADDIYNNIWSHERINPYNLSIDSLPDSVLIDCSNFVLP
                     IAGKVTSKFGPRRYRYHYGIDLRLNVGDSVKTAFGGKVRIIDYEPGGYGNYVVIRHDN
                     GLETVYAHLSKVLVEVNQDVSAGEMIALGGNTGRSTGPHLHFETRYLGHAINPAEIID
                     FENGELYDQTFLIAKNKTFRYQKERIPQGTLAKYHRVVKGDTLSFIAARYNTTVNKLC
                     KLNRISSQKILRPGEKIRVR"
     gene            8130..>9854
                     /locus_tag="P5236_00370"
     CDS             8130..>9854
                     /locus_tag="P5236_00370"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRU62854.1"
                     /translation="MKNYIVSARKYRPITFKSVVGQTALTNTLKNAIKTNHLAHAYLF
                     CGTRGVGKTTCARIFAKTINCEHLTEDYEACNECESCVAFNEQRSYNIHELDAASNNS
                     VDDIRSLIEQVRIPPQIGKYSVYIIDEVHMLSTSAFNAFLKILEEPPAHVIFILATTE
                     KHKIIPTILSRCQIYDFNRISVHEIVEQLQYIAQQENITADINALNVIAQKSDGSMRD
                     ALSIFDQMVSLCENNITYQQVLENLNILDYEYYFQLVNAFLKGDISQSLLIFNEILNK
                     GFDAYHFITGLSSHLRDILVCKDPQTIPLLEVGADIGKHYQTQAESCSSDFLFNALKI
                     TSECELNYNISKNKRLLIELTLIRLCQLTKENQPIVEEEKIPLQKIIPNDHSINSKNY
                     QKDEIQIHNEKMNSRNSVKETNNVSVKEPKQKYIQPPVSISINSSNTTSEEISEKKVT
                     PEIIQNENFTDENLVKAWKDFAETLSEEEIRMKTFIQNTEPQRLSETDFEVSVANTLQ
                     EKELKKLQPQILSYLQNQLKNSTIQMKIKLMKKTESQKPKNPEERYKTMAEQNPAITV
                     LKNGLQLEI"
BASE COUNT         3442 a         1558 c         1635 g         3219 t
ORIGIN      
        1 tcggggacac cattaatgat tttgtttttt tcgccatctt tggtaatagc tgattcagta
       61 ttaaaatcta atttttttaa atatagatta ttgtttcttg caaaagcaac atagcggcta
      121 tcaggcgaga acaaaggaga ttcctgcggt ccatattcag aaagtggctg gatttcattc
      181 ctttttatat cgtaaaggta ataatcagca ataaatgttc ttcgatagcg tttttttaca
      241 ttggtacgta ctaataattt agcttcattt ggactaaatt catatccttc aatttcctta
      301 aaaggagcgt tttgtaatcg tgaaacacta aaaagtgtat cgaccacacc acctgtttta
      361 taattgaatt tgacgatggc ttttttatta accattaagg tataattttc tccatcgttc
      421 atagaatatg gtacaactaa atcttttact ttgaatttgc catctacaat atcataaagt
      481 agattggcaa atgaaatttg tacactgaat aataaaatta aactaaaaaa aatacgtttc
      541 attttcaatt tattttttaa ctttgaataa tagctacttt acttttgttc aattttgttg
      601 aacataaaaa atattaaatt tgatatcaat tgtacaaaaa taaagcaatt tgttgattat
      661 tgaaatttcg ttttgttttt ttaccacata aagtaacgaa acaatagtga ttttttatat
      721 gaaaattaat ttgtaaacaa aacattatca gaaagaatgg ggaaaaaatt atttgaaaat
      781 aattcttcga caaaagcagt cgggatatgt ttaagtggtg gtggagcgtt gggatttgct
      841 cacattggag tattgcaatc gctagaagaa catggaattt atccattgca agtggcaggt
      901 tcaagtatgg gagctgtggt aggtgtcctg tatgcagcag gcatttcgcc acttaaaatg
      961 ttacaaatta taaaagaaga taaactttat agaataacta aattaatgaa tattcgtctc
     1021 acttctatta attcaggatt gtcaacgcat gatacattac gggaaatcat tcgggaatta
     1081 attcctcata acagttttga taaattacca aaaaaaatga atgtttgtgt atctaatttg
     1141 aatactgctg attgggaaat tgtcaataca ggtgaaaatc ttgataaatg ggtagcagcc
     1201 tcagcaagta ttccaggcat ttatgaagca atgcaaatga acaaatgttt ttatgtagat
     1261 ggaggtttat tgaataattt tccagctcaa gctcttgaac acagttgtca aaaaattatc
     1321 ggagtagatg ttattccaca ttttgaaaca cttcaattga aaaaacctac cgatactttt
     1381 tcatggtctg taagaattat ggaacataaa aattcgttac caggaagaaa actttgtcat
     1441 tttttgattg aaccgatggc aattaaaaaa tatcatggat ttagttttga tgcctaccaa
     1501 aaaatttatg aagaaggtta taaatcaacc aataattaca ttgaaaaaaa tcctgaaata
     1561 ttgaatttga aaaattaaat ttttattcat ccaagagata agctcatact tgaccgttat
     1621 caaagacatc ttgtagattt ttaaggaaaa taattaagtt ctttacctgt ttttaatcag
     1681 attcataaac tcttctcttg ttctggcttc ctggaacaca cccgtaaaat ctgaagtggt
     1741 ggtaatggaa ttaagttttt gtactcctct catttgcata cacaaatgct gtgcttcaat
     1801 aatgaccatt acacccattg gttgaagtgt gttttgaatg cattgtttaa tttgggtagt
     1861 cattcgttcc tgaacctgta aacgacgggc ataaatttcg actacacgag ctattttact
     1921 caaacccgtt atttttttat taggaatata tgctacgtga gctcttccaa aaaatggcaa
     1981 caaatgatgt tcacataatg aatagaaatc aatgtctttt acaattacca tttgacggta
     2041 attttcagta aacattgcct cacggattat ttcttctgga tcttcttcat aaccttgcaa
     2101 aaaaaattgc atagctcggg caactctttc aggagtttgt actaatccag gccgttcagg
     2161 attctctcct aaaagactga taatattttt ataatgttga gcaatttcgt tggttttctt
     2221 ttcatcaaaa attttaccac catttaactc actatccatc taaatttatt tgaaaaatta
     2281 cttattaatt tgatctctaa ctacataagt ctgatttcgc aatcgaggaa aaacctcaat
     2341 taattcattt ttaaaagcct gagcttcctc catggaggca aaatcaccca cacgcacttt
     2401 ccagaaaggc gaagaataag taacataaac accatactcc gggaaagttt cattaattaa
     2461 cctttcaata ttaaaagctt catttttagc tgttcgaggc tcattacttg aaaaaacctg
     2521 aacacggtaa ccattgacaa cagtagtttt agcaggaata gtaggaattc tcttttctgt
     2581 taaaagagtt tcgatacgtt tgtcttgatg aaaaacaaca gttccttccg tactgtcgtt
     2641 ttttgaaagt acttcaaaaa tgttttcttt ttgctgaccg cttaaagaaa aaataggaag
     2701 agagaatagc aatgttacta taaatatctg gaatagttgg tgcattttat tgaaatgata
     2761 tatggttgca aagttacaaa atgtttcttt tataactacc attaagatta atttctttat
     2821 ctttgacaaa tctcaataaa attttaagag tttttcctga aaattttttt atttttgttt
     2881 aaagataaca gaacaatttt tatgaatatt agcaggattg ctttgatagt agctggtgga
     2941 aaaggcgaac gaatgaaaac ttctattccc aaacaattta tcgaactgca agggaaacct
     3001 gtgcttatgc acactatgaa tgtttttttt cgttatgatc ctgctttatc tttttttgtt
     3061 gtacttccat ctgaacaaat tgacctctgg aagttgcttt gtcaacaaca tcatttcgat
     3121 atgcctcatc agatagtcaa cggtggtgaa acacgttttc attctgtaaa gaaaggtata
     3181 gaagctattt ctcatcaact tcaaaagaat aagaaaatta cgaataaaaa aaatgttatt
     3241 ttagttgcta tacatgatgg tgttcgtcct tttgttagta cagacaccat cgatagatgt
     3301 tttgaagaag ctgaaaagga agggaatgcc attccagccg ttgagttaac tgacagcata
     3361 agaaaagttt cacttctggg aaatgaagct gaagaaagaa gaaattacag attgattcaa
     3421 actccgcagg tatttgaatt ttcacttttg caaaaagctt atcaacagca atattctcct
     3481 ttttttactg atgatgcttc tgttgtggaa gctatgggtg ttaatattca tttggtagaa
     3541 ggtaattgcg aaaatattaa aattactact tcttttgatt tggaattagc tgaatttttc
     3601 ctaagcagaa acgaatcaag taacaggcaa attttttgat cgaattggat ttttttaatc
     3661 aagatattca atatgtaaaa ggcgtcggtc ccaaacgtgc cgaattactg aataaagaac
     3721 tcaacatttt cactgttgaa gatttattga gacattatcc ttataaatac gtggatcgaa
     3781 gccgtttcta tttcttaaat gagatttcgg aaaatatgcc ttttattcaa ttaaaaggta
     3841 aaattttaag ctttgaaaaa gcaggagaag gacggaagca acgattaata gctactttta
     3901 ctgatggctt cggcataatg gaactgattt ggtttaatgg actaaggttt gttcaagaca
     3961 aatataaaac taatatagaa tatgtagttt ttgggaagcc aactttcttt aatggcaaaa
     4021 tcagtattat ccatcctgaa atagatcttc cttcgaagtt accaccgcct gaacaaatgg
     4081 gaatgcaacc tttttacaat acaactgaaa aaatgaaatc ccattcattg aattcaaaag
     4141 ctatccaaaa aattatttat tccctattgc caactttaca aaacagcatc ccagagacac
     4201 ttccttcgta tcttttacaa cgattcaagc tcttaaatct tacggaagcc attagaaaca
     4261 ttcattttcc tcaaaactca catttactca acaaagcccg tcagcgattg aaatttgaag
     4321 aattgtttta tattcagcta agtatcgttc gtcagcaaaa atggagagaa caaaaattta
     4381 atggattcat tttcaaacat attggatatt ttttcaatac tttttatcat aaatatttac
     4441 ctttctccct gaccaatgct caaaaacggg ttattcatga aatcagaaaa gatgttggtt
     4501 caggcaaaca aatgaatcgt cttttacaag gcgatgtagg aagtggaaaa acgctggttg
     4561 ctttaatggc aatgcttatg gctgccgaca atgattttca ggcttgtatg atggtcccta
     4621 ccgaaatttt ggctcaacaa cattatacca gcattaagga attattgggc gatttaggtg
     4681 ttgaagtgtc tttattaact ggctctacac gtcaaagtga acgaaaagtt atccatcaaa
     4741 aattacaaaa cggtgaaatt catattttaa ttggcaccca tgctttgatt gaagaaactg
     4801 ttcaattcaa aaatttgggt ttggtagtta ttgatgaaca gcatcgtttt ggagttgctc
     4861 agcgagcaaa actttggact aaaaatcagc aaccccctca tatacttgtc atgactgcta
     4921 cgcctattcc acgtacgttg gcaatgacgc tttatggaga cttaaatatt tcttccattg
     4981 atgaattgcc acccggcaga aaacctattc aaacttttca tttttatgaa aacaaaaggg
     5041 ctcaactaaa caaatttatt aggcagcaag ttgaagctgg aagacaaatc tatgtggttt
     5101 atccgcttat tcaagaatcg gaagtattag atttaaaaaa tttagaagaa ggctattgtc
     5161 agttgtgcga aacatttccc caatataaaa ttagtatgat gcatgggaaa atgaaatcag
     5221 acgaaaaaga acttcaaatg aataaatttg tttcaggaga aactcaaatt atggtgtcaa
     5281 ctactgtcat cgaagtaggt gttaatgttc caaatgcttc tgtgatgatt atagaaaatg
     5341 ctgaacgatt tggtttgtcg caattgcacc agttacgtgg acgagtaggt cgtggagcag
     5401 aacaatccta ttgtatttta ttgaccaatt ataaattatc agctactact cgcaaacgca
     5461 ttgaagtgat ggtgagaact aacgatggtt ttgaaattgc tgagactgat ttacaattaa
     5521 gaggacctgg tgatctggaa ggaacgcaac aaagtggttt gccctttgaa ctaaaaatag
     5581 caaatttagc tcaagatggt aaaatgttgg aaattgctcg gcaaacagct ctggaaattt
     5641 tggaagttga tcccaactta gaaaagccag aaaataaagt tttgttgaag caactgaaaa
     5701 aaatgaaaaa tagtcccatc aattggggta taattagtta aaaaacctta aattattttg
     5761 gatttaaaat ttcatttttt tactttcgga atgatttcta taaaaagaga agtttaacaa
     5821 cttttttaaa tggaaaaaac gcttgtcatc attaagccag gagccattca gcgagagttg
     5881 attggtgaag tgataaccag attggaacga aaaggtttac acttgtgtgg tataaagatg
     5941 atgcaactaa ccgatgacat tgtttctgaa cattatgccc atttggctga tcttcccttt
     6001 tttgacagaa taaaaaattc tatgatggtt actcctatta tcgtttgttg ctgggaagga
     6061 gtagaagcag taaaagtagt gcgagcaata gtgggtgtaa caaatggacg agatgctcag
     6121 gcgggaacaa tccgtggtga ttttagtatg agcattcaag aaaatattat ccatgcatct
     6181 gatagtgttg aaactgcaaa aatagaaatt gctcgttttt tcaaaaacga cgaaattttt
     6241 gattttaaac aaaataattt gccttttata tatgcaaatg atgaaaaata attgctgagt
     6301 tatgaatgtg ttaactgatt ctccaaacat ttatttagta ctgttgattc tctttttact
     6361 tttagtaagc ggaaaaacca aatcacaaag ttttgatatg gctttaagtg ctgttggttc
     6421 tttaaaagac acaactaata tttataaaca aatgtttaat gatgattcag acgacttaat
     6481 ggaaaatcac ccagcagacg atatttataa caacatctgg tcgcatgaaa gaatcaatcc
     6541 ttacaatttg tctattgata gtttgcctga ttccgttctg attgattgta gcaattttgt
     6601 tctcccaata gcagggaaag taacatcaaa atttggtcct cgtcgttatc gttaccatta
     6661 tggaattgac ttgcgtttaa atgtaggtga ttcagtaaaa actgcttttg gagggaaagt
     6721 tcgcattata gattatgaac caggaggata tggtaattat gtggtaattc gtcatgataa
     6781 tggtttagaa actgtttatg ctcacttgtc aaaagtgttg gtagaagtga atcaagatgt
     6841 gagtgcagga gaaatgattg cattgggagg aaacacgggc agatcgacag gacctcacct
     6901 gcattttgaa acacgatatt taggacatgc tattaatcct gctgaaatta ttgattttga
     6961 aaatggcgaa ttatatgatc aaaccttttt gattgctaaa aataaaactt tcagatatca
     7021 gaaagaaaga atcccccaag gaacgttagc taaatatcat cgcgttgtta aaggagatac
     7081 actcagtttc attgcagctc gttataatac tactgtgaat aaattatgta aattgaatag
     7141 aatttcttct caaaaaatat taagaccagg agaaaaaata agagttcgtt aaattaggat
     7201 ttgcaaaaaa atatttttcc ctcaagcgat tttcttgtaa acctattgga agtaatgact
     7261 ttcatgggca aaaaaaataa aaaatagttt ttaaaaattg ataaaaaata tccttgagta
     7321 cttagaggat attcattaca aatacaatgc ccgccgcccg gcttttacag gggtaagcca
     7381 agagtaatct tattttattc atattataat cgtttttacc ttgtccaaac ttgccttcaa
     7441 cccaaattgt cattagaaaa taagctataa ttatctgaac tataatatat tgtatcgaga
     7501 aaaacattag caaaattaag cttgtttttc aaaaatgaaa gtaagaaatt cttacttatt
     7561 tataaaaagc ccctagataa aaattgtcta aaatttactg aatttgtatt aggtactttg
     7621 cctcgactcc tctcattttt atttgttttt tagatttttt tctgttccac tccgtgcctt
     7681 ctctgtgaaa ccctgtgaag atgaatgatt tattacacag agggacacag ctattttttc
     7741 ttttttcgct ccaaactcat gactcaaaat tcgcacctcg tatctcgaat cctgcacctc
     7801 gcaaactcga aaccgatcaa ttttcagctc atcagacgaa atatatctaa aaagatcgtt
     7861 aatcggttaa atgcaacttt aatagaaaaa atatcatcgt ttatcatgca aaaaaaattg
     7921 gaatgatttt caatacttta aaaactttat atttaagtga acgatatatt tctaatgaca
     7981 attctcagtt aaattttcct aataaataat atcaacccaa tgataacaag ttctttataa
     8041 tcttttattt ctttcaaaaa aaatttcacg ggttcaaaac ctaaattttt ttcattaaat
     8101 ttgcaagtaa aagattgaaa aatttgttta tgaaaaacta tattgtttca gcaagaaaat
     8161 atcgtccaat caccttcaaa tcggtagttg gacaaacggc tttgacaaac actttaaaaa
     8221 atgccataaa gaccaatcat ctggcacatg cctatctgtt ttgtggaacc agaggcgttg
     8281 gcaaaaccac ctgtgcacgg atttttgcaa aaactattaa ttgtgaacat cttacagagg
     8341 attatgaagc ctgcaatgaa tgtgaatcat gcgttgcttt caacgaacaa cgttcctaca
     8401 atattcatga actggatgct gcttccaata atagtgtgga cgatattcgt tccttaattg
     8461 aacaggttcg cattccgccc caaataggga aatacagcgt gtatatcatt gacgaagttc
     8521 acatgctttc taccagtgcc ttcaatgctt ttctaaaaat tctggaagag ccacccgcac
     8581 atgttatatt tattctggca accacagaaa aacataaaat tattccaacg attctctctc
     8641 gttgtcaaat ttatgatttt aatagaattt ctgttcatga aattgttgag cagttacaat
     8701 atattgctca acaggaaaat atcacggctg atatcaatgc tcttaatgtg atagctcaaa
     8761 aaagtgacgg gagcatgcgt gatgctttat ctatttttga tcagatggta agtctgtgcg
     8821 aaaataatat tacatatcaa caagtgttgg aaaatttaaa cattctggat tatgaatatt
     8881 attttcaatt agtaaatgct tttttaaaag gagatattag tcaatcactt ctgattttca
     8941 atgaaatttt gaacaaagga tttgatgcct atcattttat tacaggactt agttctcatt
     9001 tgcgcgatat tttggtttgt aaagatccac aaactattcc tttactggaa gtgggtgctg
     9061 acataggaaa acattatcaa acacaggcag aaagttgctc ttctgatttt ttattcaatg
     9121 ccttaaaaat aaccagtgaa tgtgaactta attataatat cagcaaaaac aaacgattgt
     9181 tgatagaact tacgctaata cgtttgtgtc aattaactaa ggaaaaccaa ccaattgttg
     9241 aagaagaaaa aatacccctg caaaaaatta tacctaacga ccattctata aattcaaaaa
     9301 attatcaaaa ggacgaaatt cagatacata atgaaaaaat gaattcaaga aattctgtaa
     9361 aagaaactaa taatgtttca gtgaaagaac caaaacaaaa atatatccaa ccacccgtaa
     9421 gcatttctat caattcttct aatacaactt cggaggaaat ttcagaaaaa aaagtaaccc
     9481 ctgaaatcat tcaaaatgaa aattttacgg atgaaaattt ggtgaaagcc tggaaagatt
     9541 ttgctgaaac actttctgaa gaagaaatca gaatgaaaac ttttattcaa aatacagaac
     9601 ctcaacgctt atcggagaca gatttcgaag tttcagttgc taatactctt caggaaaaag
     9661 aattaaaaaa attacaacca caaattttaa gttatttgca aaatcagtta aaaaattcaa
     9721 ccattcaaat gaaaattaaa ttaatgaaaa aaactgaatc gcaaaagcca aaaaatcctg
     9781 aggaacgcta taaaacgatg gctgaacaaa atcctgcaat tacggtcctt aaaaatggtt
     9841 tgcaactcga aata
//
LOCUS       DAOTKS010000008         9788 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_7216_length_9788_cov_4.804377, whole genome shotgun sequence.
ACCESSION   DAOTKS010000008 DAOTKS010000000
VERSION     DAOTKS010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9788
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7216_length_9788_cov_4.804377"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="P5236_00375"
     CDS             complement(<1..659)
                     /locus_tag="P5236_00375"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HRU62855.1"
                     /translation="MATLGKIRSKGILLVVVVGLALLAFIIGDFLNSSTSYFNKSREK
                     VAQIAGENINIRQFSEAIEQMTQVYKIETGQTELNEDIISQIRASVWESLVNEIILNK
                     EAKKLGLTVSPEELSDQLIGYNIHPLILQRRVFADENGQFSRSLLIQFLNSFDATPTS
                     NEMKQQIDMAKNYWLFWEKNVKNSILQEKYNKLITSAVTANGVDAKMNYEARKFTVDS
                     W"
     gene            complement(819..2066)
                     /locus_tag="P5236_00380"
     CDS             complement(819..2066)
                     /locus_tag="P5236_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRU62856.1"
                     /translation="METLPAILLTLLFSAFFSGMEIAFVSSNKLRFELDKKQRSLSSY
                     FLSIFYEHPNQYISTMLVGNNIALVIYGLLMAKILNPLFEPIGNQFIITFLQTIIATA
                     IVLITGEFLPKTIFRINSYLWLRVFSGILFFFYVVLYPITYFVTWLSFSVLHLFGVKT
                     SDASREKVFSRADLNYLIQESFENKNDEKEINKEVEIFQNVLDFSNVKLRDCLVPRTE
                     IVALDYEVDEETLKQTFIETGFSKIPIYKGNIDNIIGYIHSSEMFVHPKDWRDHLNQI
                     PIVPENMSAQKLLKIFLKEKKTIAVVIDEFGGTAGIVTLEDIIEEIFGEIEDEHDTHE
                     YTCEQLNEKEYLFSGRLEIKEINEKFNLNIPESDNYETIAGFILYHLERFPKLNEVIQ
                     IDKFTLKCIEIKNNRIELVKLSL"
     gene            2231..2303
                     /locus_tag="P5236_00385"
     tRNA            2231..2303
                     /locus_tag="P5236_00385"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2264..2266,aa:Lys,seq:ttt)
     gene            complement(2474..4465)
                     /locus_tag="P5236_00390"
     CDS             complement(2474..4465)
                     /locus_tag="P5236_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HRU62857.1"
                     /translation="MKTYRQLTKKEITSLTLQGCLAEDWNQIKVTPDFNSQFVVNVHF
                     SGEIFLEGFQKIFELPGGVKKHAGIYNCRLHNVTVEKDVYINRIHNYIANYTIGEGSY
                     IENVNLLVVDCWSTFGNGVRVPVMNEGGGREIPIFDYLSAPLAYILTLYRHRPTLIQN
                     LQKLIDDYASSIGSSKGSIGQNVHIINCGTIRQVRIGNYANIEGASVLFNGSINSTAL
                     APITIGAGVKCNDFIISSGVSITDSTLVSRCFIGQGCILGKHYSALDSLFFSNCQGMH
                     GEATAIFAGPYTVSHHKSTLLIAGMFSFLNAGSGSNQSNHMYKLGPIHQGMAERGAKT
                     TSDSYLLWPAKIGVFTLVMGRHTKHSDTSDLPFSYLIEKGTESILVPGINLRSVGTIR
                     DAQKWPKRDNRKDTRKLDPINFNLLSPYTIQKMIKGVEILRKLQKISGETTEIYTYQN
                     CTIKKLALLKGIDLYKMGIYKFLGNSLISRLNEKEFSNIEEIRQYLIPDTQTGRGEWI
                     DLAGLIAPKSEVENLIKKIEVGTINLSEIQLEFEKMHQEYYTYEWTWAKEKLEIHWGK
                     PIEEVTIDEIISMIELWKKSVITLDELIYEDAKKEFSLNAKTGFGVDGDEEQKNLDFE
                     SVRGSFENNPFVLEVINHIKIKRELGDGLISRLEKCRMN"
     gene            complement(4500..5702)
                     /gene="hflX"
                     /locus_tag="P5236_00395"
     CDS             complement(4500..5702)
                     /gene="hflX"
                     /locus_tag="P5236_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRU62858.1"
                     /translation="MENETNEEIAALVGLITPIQNEEKASEYLEELAFLTETAGAKPQ
                     KIFLQRLNCPDPNTFIGPGKLIEIKSYIIENNINVVIFDDELSARQLRNLEKELNVKI
                     LDRTNLILDIFAKRAKTAVAKTQVELAQYQYMLPRLKRMWTHLERQQGGIGTRGPGET
                     QIETDRRIILSKIATLKKKLKEIEQQTITQRKNRGKLVRVALVGYTNVGKSTLMNLLS
                     KTDLLAEDKLFATVDTTVRKVIIHNLPFLLTDTVGFIRKLPHQLIESFKSTLDEVREA
                     DLLLHIVDISHPNFEEQIQIVNQTLKEICGEEKPMILVFNKIDAFPYKPKEADDLSPM
                     QRESISLEELEKSWMAKINEDCVFISAKEKQNIDKLKELMYKKIKAIHIQRYPYNDFL
                     YPDDLSAN"
     gene            complement(5808..6491)
                     /locus_tag="P5236_00400"
     CDS             complement(5808..6491)
                     /locus_tag="P5236_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007751643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRU62859.1"
                     /translation="MDIKKSSKASLEDKKSIFILLGLVIALSFLYIGLEWTQREIKVY
                     EDVSTELITEEELDIIQTTQPETPPPPPPPAPEVVEVLNVVENTAETQTINIQTEDIK
                     NKPIDIVAAPVAAPIQEEDEQVIFQVVEEMPHFPGGEQALMKWLSENIRYPVIAQENG
                     IQGRVICQFVVNTDGSIVDIQVVRGVHPSLDEEAVRVIKSMPKWVPGKQRGKPVRVRF
                     TLPINFKLQ"
     gene            6886..7893
                     /gene="porQ"
                     /locus_tag="P5236_00405"
     CDS             6886..7893
                     /gene="porQ"
                     /locus_tag="P5236_00405"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HRU62860.1"
                     /translation="MKRKISLSLLFWLNINILIYPQAGQGVYKFLDLPVSSRIAGLGG
                     TNVSIRDNDLNFAFRNPALLTANTHHSIALNYANYLSDIQFGSVIFGENFKDKHYTAY
                     GIQYVDYGTFKETTEQNEIIGEFTAKDMAFYIMYAYPLNEKITVGGTFKPVYSVYERY
                     SSFGLALDAGISYNDINNLFSLGLVLRNVGTQLKGYYSDEEGQHYEALPFDIQLGATK
                     KLVHAPLRFSFTLHNLQNWDMEYLSSTGTKKIDFFDNLFRHFIVGLEFIPSNNFYLIA
                     AYNYRQSKEMKIEDFKSMAGFSFGGGVKLYKFQVGFGVNTFQVGNYSYQFSIATSLDA
                     F"
     gene            7988..9139
                     /locus_tag="P5236_00410"
     CDS             7988..9139
                     /locus_tag="P5236_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRU62861.1"
                     /translation="MAKILVIDDERSIRNTLKDILEFEKHKVSLAENGKQGLEIAQNE
                     AFDLIFSDIKMPEIDGIELLTLLKEREIEVPIVMISGHGTIDTAVECIKKGAFDFIEK
                     PIDLNRLLITVRNALERKELITESKTLKKNTEKKYQIIGQSPAIEKVRKLIDRVASTD
                     ARVLITGDNGTGKELVARQLHQKSNRANAPFIEVNCAAIPSELIESELFGHEKGAFTS
                     ALKQRIGKFEQADEGTIFLDEIGDMSLSAQTKVLRILQENEFTRVGGDKIIKVNVRVI
                     AATNKNLINEIEKGNFREDLFHRLNVIPIHVPSLEERKEDIPLLITHFSELICSEQGR
                     QVKEFEPEAVSLLQQRNWPGNVRELRNIVERLIILGGSKITKEDVELFV"
     gene            9174..9623
                     /gene="rpiB"
                     /locus_tag="P5236_00415"
     CDS             9174..9623
                     /gene="rpiB"
                     /locus_tag="P5236_00415"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HRU62862.1"
                     /translation="MKPLTELKIAICSDHAGYELKNELIEYLKIKNLAALKDFGTYSA
                     ESCDYPDYAHPLATAVENGEFDFGIALCASGNGVNITVNKHKGIRGALCWMSEIAFLA
                     RKHNDANVISLPAHFISQEEAKKMIDIFLTTDFEGGRHQRRIEKIPY"
BASE COUNT         3191 a         1545 c         1532 g         3520 t
ORIGIN      
        1 taccagctat cgacagtgaa ttttcttgct tcataattca ttttcgcatc aacaccattg
       61 gctgttaccg cagaagttat taatttattg tatttttctt gaagaatgct attttttaca
      121 tttttttccc aaaacaacca ataatttttt gccatatcaa tttgctgttt catttcgtta
      181 ctggtaggag ttgcatcaaa actatttaaa aattgaatca ataacgaccg gctaaattga
      241 ccattttcat cagcaaaaac acgccgttga agaatcaaag gatgtatatt atatccaatc
      301 aattgatcag aaagttcttc agggcttact gtgagtccta atttttttgc ttccttattt
      361 aaaattattt catttaccaa actttcccat acgctagcac gaatttgtga aataatatct
      421 tcatttagtt cagtttgacc tgtttcaatt ttgtacactt gtgtcatttg ctcaattgcc
      481 tcactaaatt gtctaatatt tatgttttct cctgcaattt gagctacttt ttcacgtgat
      541 ttgttaaaat aagaggtact cgaatttaaa aagtcaccaa tgataaatgc taataatgct
      601 aaccctacta ctacaaccag taaaattcct ttacttctga ttttccctaa tgttgccatt
      661 ttttaaattt aaaaattatt cactaaattg ttctactgcc gattttgaga gcacgaatat
      721 acaaaaaaat tattcaactt ttttattttc tatttcctta attttttaga attcatttga
      781 ggcttaatta attatctaaa tatcaaaata ttataatttt agagagataa ttttactaat
      841 tcaattcgat tattttttat ttctatacat tttaaggtaa atttatcaat ttgaatgact
      901 tcgtttagtt taggaaaacg ttcgagatga taaaggataa atccagctat tgtttcataa
      961 ttgtctgatt caggaatatt taaattaaat ttttcattaa tttccttgat ttctaatctg
     1021 cctgagaaca gatattcttt ttcattcagt tgttcacacg tatattcatg tgtatcatgt
     1081 tcgtcttcta tttctccaaa aatttcctca ataatatctt ccagtgttac gatacccgcg
     1141 gttcctccaa attcatcaat cacaactgca atagtttttt tctcctttag aaaaattttt
     1201 aacaattttt gagcagacat gttttctggt acgataggaa tttgatttag atgatcacgc
     1261 cagtcttttg gatgaacaaa catttcagaa gaatgaatat aaccaataat attatctata
     1321 tttcctttat aaatgggaat ttttgagaag cctgtttcaa taaaagtttg tttcagagtt
     1381 tcttcatcaa cttcataatc caatgcaacg atttcagttc gaggcactaa acaatcgcgg
     1441 agtttcacat ttgaaaagtc taacacattc tgaaaaattt ctacttcctt attaatctcc
     1501 ttttcgtcat ttttattttc aaaactttcc tgaatcaaat aattcaagtc tgcccgtgaa
     1561 aatacttttt ctcgggaagc atcactggtt ttaactccaa ataaatgaag tactgaaaat
     1621 gataaccatg ttacgaaata agttatagga tacaaaacga cataaaagaa aaacaaaatg
     1681 cctgaaaata ctcgcaacca taaataagaa ttgatacgga aaatcgtttt gggcaaaaat
     1741 tcacccgtaa tgagtacaat tgcagtagca ataatggttt gtaaaaaagt tattataaat
     1801 tgattaccta taggttcaaa cagtgggttt aatatttttg ccatcaataa tccataaatg
     1861 acaagagcaa tattatttcc taccagcata gtagaaatat attgattagg gtgttcataa
     1921 aaaatggata aaaaataaga acttaaacta cgttgttttt tatctaattc gaaacgtagt
     1981 ttatttgatg agacaaaagc aatttccatt cctgaaaaga aggcggaaaa caataaagtt
     2041 aataaaatgg caggtaaagt ttccacacaa aataatttcc taattattta caaagataat
     2101 atttatacta tgaatcatca aaaagcaaaa ttcttttcaa ttttgtgttt gaaggaagta
     2161 actgataaga aataattgaa ttaactttga ttttttttaa aaaaaatgta tctttgcaac
     2221 ctcttttgaa gatttagtag ctcagcaggt agagcacatc ccttttaagg atggggtcct
     2281 gggttcgaat cccagctaga tcacaagtta ttctttcctt ataaaacttt ctttttttac
     2341 tatattcttc agttattcga caaaaatcat tgtttttgct attttcgttt ttcctcagga
     2401 aacgaaaaag cataaaaaat ttttcaactt cccatgctat acataaattt ttttgaaaaa
     2461 tgagctggat gttctaattc attctacatt tttcaagacg tgaaataagt ccatcaccta
     2521 attccctctt aatttttata tgatttataa cttccaggac gaaaggatta ttctcaaaac
     2581 ttcctcttac tgattcaaaa tcaagatttt tttgttcttc atcgccatct acgccaaaac
     2641 cagtttttgc attcaggctg aattcttttt tggcatcttc gtagattaat tcgtccaacg
     2701 taattactga ttttttccac aattcaatca tacttataat ttcatcaata gtaacttctt
     2761 caataggttt tccccaatga atttccagtt tttcttttgc ccacgtccat tcatatgtat
     2821 aatattcttg atgcattttt tcaaattcta actggatttc cgacaaattt attgttccaa
     2881 cttcaatttt tttaatgagg ttttcaactt cactttttgg agcaattaaa cctgccaggt
     2941 caatccattc accccgtcca gtttgcgtat caggaataag gtactgtctg atttcttcta
     3001 tattactaaa ttccttttcg tttaatctac tgatcaatga atttcccaaa aatttataga
     3061 ttcccatttt ataaaggtcg attcctttta ataaagccaa ttttttaata gtacaatttt
     3121 gataagtata aatttcagtc gtttcacctg aaattttctg aagttttcta agaatttcta
     3181 caccttttat cattttttga atagtataag gactcaatag attgaaatta attggatcta
     3241 actttctggt atcttttcga ttatctctct taggccattt ctgagcatct cttatcgtac
     3301 caacgcttcg caaattaata cctggtacca aaatactttc agtacctttt tcaattagat
     3361 aggagaaagg caaatcggaa gtgtcagaat gctttgtatg acgtcccata acaagtgtaa
     3421 atacgccaat tttggctggc cagagtaaat aggaatcact ggttgttttt gctcctcgct
     3481 cagccatgcc ttgatgaata ggacctaatt tatacatgtg attgctttga ttggacccac
     3541 ttcctgcatt caaaaaagaa aacattcctg caatcaataa agtagatttg tgatgcgaaa
     3601 cagtataagg tccagcaaaa atcgctgttg cttcaccgtg cattccttga cagtttgaaa
     3661 agaaaagtga atcaagggct gaataatgtt tacccaaaat acagccttgt cctataaagc
     3721 agcgagaaac taacgtagaa tcggtaatag aaactccaga actaatgata aaatcattgc
     3781 atttaacacc tgcaccaata gtgataggag ctaaggcggt gctattaata cttccattga
     3841 ataatactga agctccttca atgttggcat aatttcctat tctaacttgc cgaatggtgc
     3901 cacaatttat gatatgaaca ttttgtccta ttgaaccttt actggaaccg atagaagaag
     3961 catagtcatc tattagtttc tgaagattct gaattaaggt aggtcgatga cgataaagtg
     4021 ttaaaatata agctaatgga gcggatagat aatcaaaaat gggaatttcc cgtcctccac
     4081 cttcattcat tacaggaacc cttacaccat ttccaaaagt tgaccagcaa tctaccacta
     4141 ataaattgac attttcaata tatgaaccct caccaatggt ataattggcg atgtaattat
     4201 gaattctgtt aatgtagaca tctttttcaa ccgttacatt gtgaagacga caattataaa
     4261 tgccagcatg ttttttgact ccgccgggca gttcaaaaat tttctgaaaa ccttccaaaa
     4321 atatttctcc tgaaaaatga acatttacaa caaattgact attgaaatcg ggagtaactt
     4381 ttatttgatt ccaatcttct gcaaggcaac cttgtaaggt gagcgaagta atctcttttt
     4441 tagtaagttg acgataagtt ttcatagtta aaagttatta ggaattggtt tgttattttt
     4501 tagtttgcag ataaatcatc tggatataaa aaatcattgt acggataacg ttgaatgtga
     4561 atagctttta tttttttgta cattaactcc tttagcttgt caatgttttg tttttctttt
     4621 gcagaaataa atacacaatc ttcattgatt ttagccatcc agcttttttc caattcttcc
     4681 agactgatgc tttcacgttg cataggtgaa agatcgtcag cttctttcgg cttataagga
     4741 aaagcatcaa ttttattaaa aactaaaatc atgggtttct cctcaccgca aatttctttc
     4801 agtgtttgat taactatttg aatttgttct tcaaaattag gatgggaaat atccacaata
     4861 tgtaataaca agtcagcttc acgtacttca tctaaagttg atttgaacga ttcaataagt
     4921 tgatgaggta atttacggat aaatccaacg gtatcagtaa gtaaaaaagg caaattatga
     4981 ataattactt ttcgtaccgt agtatcaact gtagcaaaaa gtttatcttc tgccaggaga
     5041 tctgttttgc taagtaaatt cattaaagtt gattttccta cgttcgtata gcctactaaa
     5101 gcgacacgta ctaattttcc tctatttttt cgttgggtta tggtttgttg ctcaatctct
     5161 tttaattttt tctttaaagt ggcaattttt gaaagaatga tccgccgatc agtttctatt
     5221 tgtgtttcac caggaccccg agtgccaatt cctccttgtt gtcgttccag gtgtgtccac
     5281 atacgtttta agcgaggtaa catatattga tattgtgcca attcaacttg agttttggca
     5341 actgcagttt tagcacgttt ggcaaaaata tctaaaatta aattggttct gtctaaaatt
     5401 tttacattga gttctttttc cagatttcgg agttgtcttg cgcttaattc atcatcaaaa
     5461 ataactacat taatattatt ctcaatgata tatgatttta tttctatcaa ttttcctgga
     5521 ccaataaaag tatttggatc aggacaattg agacgctgaa gaaaaatttt ttgtggcttg
     5581 gctcctgctg tttcggttaa aaaggctagt tcctccaagt attcggatgc tttttcttca
     5641 ttttggatcg gagttatcag tccgactaac gctgctattt cttcatttgt ttcgttttcc
     5701 aaagttgaaa ttcctttatt aaaaaaccgt ttggagctac caaacggttt taaatttttc
     5761 gaagtcaata ttaaatcata agactaactt cctatctcga ttaataatta ttgtaattta
     5821 aagttgatag gaagagtgaa tcttactcgt acgggctttc cacgttgttt ccctggaacc
     5881 catttaggca tagatttaat aactcgcact gcttcttcat ccagacttgg atgaacacct
     5941 cttaccactt gaatatcaac tattgaacca tcagtgttta ctacaaattg gcaaattaca
     6001 cgtccttgaa ttccattttc ttgagcaata actggatacc tgatattttc actcaaccat
     6061 ttcatcaaag cttgttcacc tcccgggaaa tgtggcattt cttcaactac ctggaagata
     6121 acttgttcat cttcttcttg tataggagct gcgactggtg ctgctacaat atcaatcggt
     6181 ttatttttta tatcttctgt ctgaatgtta attgtttgtg tttcagcagt gttttctaca
     6241 acatttaata cttctactac ttctggtgca ggaggtggag gagggggagg agtttctggc
     6301 tgtgttgttt gaataatatc gagttcttct tcggtaatta actcagtact tacatcttca
     6361 taaactttaa tttcacgttg cgtccattcc aatcctatgt aaagaaagga taaagctatt
     6421 actaaaccca ataaaataaa aattgatttt ttgtcttcta atgaagcttt agacgatttt
     6481 ttgatatcca taattataag gtctttttat aaatttttca gatggagcaa atgtacaatt
     6541 tttttttaaa atgtttatat aattaataaa gaaaaaatat ttattttttc ttctatggca
     6601 aaattattca tttctcttga aatatttatt tttagatgta ctttttttta tttttaacgt
     6661 ttatattttt agaattgaat tgtaagttta ctctgaaaaa ttaaattaaa taattttgtt
     6721 gtttgtaagt gattgtatat cagcataaaa tttttaaata taattaaaaa aatagttttt
     6781 atgagtaaac tcgatcttaa aaaatttttt ccaaataatt aaaatgatat acctttaaaa
     6841 attttttaag agcagcttaa tttttttaga attaatattt cttgaatgaa aagaaaaata
     6901 agtttatctc tacttttctg gctaaatatc aatatcttaa tatatcctca agcaggacaa
     6961 ggtgtttata agtttttaga tttacctgtt tcttctcgaa tagctggttt aggcggcacc
     7021 aatgtttcta tcagagacaa tgacttgaat tttgcttttc gaaatccagc tttactaaca
     7081 gccaacactc atcattcaat agcgttaaat tatgcaaact atctttctga tattcagttt
     7141 ggtagtgtta tttttggaga aaattttaaa gacaagcatt atacagccta tggcattcag
     7201 tatgtagatt atggaacttt taaagaaact accgaacaaa atgaaataat tggcgaattt
     7261 acagcaaaag atatggcttt ttatatcatg tatgcttatc cgttaaatga aaaaattaca
     7321 gtaggaggaa cttttaagcc agtttattcc gtatatgaaa ggtattcaag ttttggactg
     7381 gcgttagatg caggcataag ttataatgat ataaataatc tgttttctct tggtttggtt
     7441 ttacgaaatg ttggtacaca attaaaaggt tattacagtg atgaagaagg acaacattac
     7501 gaagctttac ctttcgatat acaattggga gctactaaga aattagttca tgcgccgttg
     7561 cgtttttcat tcactttaca taacttgcaa aattgggata tggagtatct ttcttccact
     7621 ggaacgaaaa aaatagattt ttttgataac ctttttcgtc attttattgt tggtttagaa
     7681 tttattcctt cgaataattt ttacctaata gctgcttaca attatcgaca gagcaaggaa
     7741 atgaaaattg aagatttcaa aagtatggca ggtttttctt ttggaggagg cgtaaaatta
     7801 tataaatttc aggtcgggtt tggggttaat acttttcaag taggaaatta ttcgtatcaa
     7861 ttttcaatcg caacttcatt agatgcattt taatgttatt atgttgtaaa aaattcttat
     7921 ttttaagttt ttaaaaattt tgaatttcaa tttgtttaaa ataaacacaa tggaatagga
     7981 taaaatcatg gcaaaaatat tagtaattga tgatgaacgc agtatccgta atacactaaa
     8041 agatattctc gaatttgaaa aacataaagt atctttggct gaaaatggca aacaaggttt
     8101 agaaattgct caaaatgaag catttgactt gattttttct gatataaaaa tgccagagat
     8161 agatggcatt gaattgttaa cgcttttgaa agaaagggaa attgaagttc ccattgtaat
     8221 gatttctggg catggcacta ttgataccgc agtggaatgt atcaaaaaag gtgcatttga
     8281 ttttatagaa aagcctattg atttgaatcg tttattaatc acagtgcgta atgctttaga
     8341 gcgaaaagaa cttataacag aatcgaaaac attaaagaaa aatacagaaa agaaatatca
     8401 aattatcggt caatcgcctg cgatagaaaa ggtaagaaag ctgattgatc gtgttgcttc
     8461 aactgatgca cgagttttaa tcactggtga taatgggaca ggaaaagaat tggttgccag
     8521 gcaactgcat caaaaaagca atcgagcaaa cgcccctttt attgaagtaa attgtgcagc
     8581 aattccttct gaattaattg aaagtgaatt atttggtcac gaaaagggag cttttacttc
     8641 agctctcaaa caaagaattg gaaaatttga acaagccgat gaaggtacta tttttttaga
     8701 cgaaattgga gatatgagtt tatcagctca gacaaaagtg ctccgaattt tgcaggaaaa
     8761 cgaatttaca cgtgtaggag gtgataaaat cattaaggta aatgtgaggg ttattgcagc
     8821 tacgaacaaa aatctgataa acgaaattga aaagggtaat tttcgcgaag atttatttca
     8881 tcgtttaaat gttattccta ttcatgttcc ttcactggaa gaaagaaaag aagatattcc
     8941 actcttgatt actcatttta gtgaactgat ttgttccgaa cagggaagac aggtcaaaga
     9001 gtttgaacca gaggctgtat ctttattgca gcaaagaaat tggcccggta atgttcgtga
     9061 acttcggaat attgttgaac gtcttattat tttaggagga tctaaaatta ccaaagaaga
     9121 tgtagaatta tttgtttgat tttttaaata tttgaattta aacaaaatca attatgaaac
     9181 cattgacaga attaaaaata gccatttgca gcgatcatgc aggttatgaa ttaaaaaacg
     9241 agctgattga atatttgaaa ataaaaaatt tggctgcttt aaaagatttt ggtacttaca
     9301 gtgcagaaag ctgcgattat cctgattatg ctcatccatt ggcaactgcg gtagaaaatg
     9361 gtgaatttga ttttggtatt gccctttgtg caagtggtaa tggtgttaat ataacagtaa
     9421 ataaacataa aggaatacgt ggtgctcttt gctggatgtc agaaattgct ttcttagctc
     9481 gaaagcacaa tgatgccaat gtaatttctt tacctgctca ttttatttct caagaagaag
     9541 caaaaaaaat gattgatatt tttctgacaa ctgattttga aggtggaaga catcaacgcc
     9601 gcatagagaa gataccatat tgattttttt gatagttatc tttttaaacg gacagtttat
     9661 aaaagttttg gtggaagagt aatcgaaaga tgagaaaatt ctctttgagt ttttccatca
     9721 ttttttcaaa ttccaattta ttgatattta atcgaataga acatatttaa ggagcgacta
     9781 agtaagta
//
LOCUS       DAOTKS010000009         9560 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_7394_length_9560_cov_4.344240, whole genome shotgun sequence.
ACCESSION   DAOTKS010000009 DAOTKS010000000
VERSION     DAOTKS010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9560
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7394_length_9560_cov_4.344240"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..1407
                     /gene="ftsA"
                     /locus_tag="P5236_00420"
     CDS             154..1407
                     /gene="ftsA"
                     /locus_tag="P5236_00420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRU62863.1"
                     /translation="MNGRTYIALNLGSSQISALAAEVLDNGAVRILGSESKISEDVKH
                     GIIEHPSGCAFKVNELLKLLQNSSKIHDIQQVCVGINARSMKVISGLVTRFVGQNKMV
                     TDMLINEMKDECARKYSQNDLIAFQTIPVKYQLDEEYITNPVGKKGVQITGYYNVVIG
                     NKLIKERFDGCFDRTGIMVEYSPLAIESLATVLMEEDERKNGCALIDFGATTTTLGVY
                     KNDILQQLLVVPLGGENITSDIEELGIKKSYAERLKCLKGVSVVKFVENPIYVEMPSE
                     NAEENLRISTSFLANIINARLEEITQPIFKTINQLPFSLEGGIIITGGASKLNYLKEF
                     IEENTGLPVRFGNHSEWLDDKTPEKFFDDTYSQLIGTIVLTDEYRKLNPTNGQSRKEP
                     KIKRNFKDKLTEHIISLFDEDSEMK"
     gene            1446..2750
                     /gene="ftsZ"
                     /locus_tag="P5236_00425"
     CDS             1446..2750
                     /gene="ftsZ"
                     /locus_tag="P5236_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRU62864.1"
                     /translation="MEELIEMLPLELPLVNNSIIKVIGVGGGGGNAVNHMYRQGITDV
                     SFVICNTDNQVLIKSPVPIKIQLGVNTTAGLGAGGNPEVARQAAEESIDKIKEVLQDN
                     TKMVFITAGMGGGTGTGASPVIAKVAHDMGILTVGIVTIPFAFEGNLKIKQALEGVAA
                     LSEHVDAILVINNEKLKDIYPDLELSNAFAKADDVLTNAAKSIAEIITVPGYINTDFA
                     DVYSIMKDGKVAIMNTGYASGENRITKAIENALNSPLLNTNDVNGASKVLLSLYCSTT
                     NQIKMEEVQQIHDFMKKVGRNVQVIWGATFDDTLGDNVKITLIATGYDVSDIPGMPTS
                     IVNKHKNSQFAEKKYSTIDEKKPPIAHSTEEEKELQIQKAMDEYYGDDISQNTSEKEL
                     KVGIEDNIPVVNFKDLEDLENDEILKKVEKIPAWIRKQKKND"
     gene            2785..3240
                     /locus_tag="P5236_00430"
     CDS             2785..3240
                     /locus_tag="P5236_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRU62865.1"
                     /translation="METLFDKISEDIKQAMLARDKVTLEALRGIKKEFLEAKAAKGND
                     GTLSDEAAVKILQKMIKQRKESAQIYAEQNRLELAEAELAEVAVIEKYLPPQMDEEQL
                     KTEINKIIDEVGAKSMQEMGRVIGIAVKKLSGKADSQMIANTVKSLLVK"
     gene            complement(3312..4514)
                     /locus_tag="P5236_00435"
     CDS             complement(3312..4514)
                     /locus_tag="P5236_00435"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HRU62866.1"
                     /translation="MKILVLNCGSSSVKYKLFNLETNEILGSGIVEKIGMKGSCLRHM
                     RHDGQKILLEGEVLEHQTAIEYILGVLTSKRHGTLKSLEEIDAVGHRVVHGGEKFNKS
                     VLITDEVIRKIEECIDIAPLHNPPNLAGIHAIEELLPSVPQVAIFDTAFHQTMPKYAY
                     MYAIPYSLYTKYSIRRYGFHGTSHRYVSKRACDFLGLDYQQTKIITVHLGNGASATAI
                     KNGKSIDTSMGFTPLEGLMMGTRSGDVDLGIATFLMEKEMIGPSTLSTLFNKHSGMLG
                     ISGISSDMRDIKAAIEKGNERARLALEMYEYRIIKYIGAYTAALNGVDLLAFTGGVGE
                     NQASTRTNVCNNLNYLGVKIDEKINMATQGKEALISTPDSSIKVAVIPTDEEFMIASD
                     TYEIVKGL"
     gene            complement(4511..5485)
                     /locus_tag="P5236_00440"
     CDS             complement(4511..5485)
                     /locus_tag="P5236_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HRU62867.1"
                     /translation="MHEIIVEPIEKYGLSKRSRRRTLFSKIGIVGCGYEGSAIATRAL
                     LNGIEVVFLEPTDEKIANAFSRIEQKLNKKIENWGLTENEKKIVLSRVQGTKNYKDFS
                     DCDFVIEAVRYDDQTGERHVKERKEVFLQLEKVLSPQAIIASNVSTVVITELASELRY
                     KERCVGLHFLSNIPESNIIEIVRGLYTSDETYNKVCQFARLINHQYVAVAESAGLVSI
                     RLFLIELNEACEILMEGISTVEDIDRVLTVGFGHRLGVFRTADQMGIEKIVNLMENLF
                     NEYGNSKYKPSPVLLRLYRAKHFGVRKGKGFYKYDEEGNIIKQGGEKA"
     gene            complement(5533..>6399)
                     /gene="pta"
                     /locus_tag="P5236_00445"
     CDS             complement(5533..>6399)
                     /gene="pta"
                     /locus_tag="P5236_00445"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HRU62868.1"
                     /translation="ILLGKEEKIRKLAEENHLKSITEAKIIDPENYPKLNEYINLLYK
                     IRKNKGITYEEATRLAKDPLYFGCLMIKNGDADGELAGARNFTTHVLRPAFQIIKTVP
                     GINVVSGLFLMFTPTHYGENGLLIFADCAVNPCPTAEELAQIAMCTAETAHNLAEIEP
                     RVAMLSFSTKGSAQHELVDKVIKATKIAKEMNPDLLIDGELQADAAIVPSVAERKVPD
                     SPVAGRANVLIFPDLQASNIGYKLVERLSGSQAIGPILQGMAAPVNDLSRGCSVNDIV
                     QMIIITSNLVRK"
     assembly_gap    6400..6499
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6500..8524)
                     /locus_tag="P5236_00450"
     CDS             complement(<6500..8524)
                     /locus_tag="P5236_00450"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRU62869.1"
                     /translation="MVTLSTICLASEKNQLIGKSTIKIENGKMTPEVLWSFGRISGVQ
                     LSPDKTKILYHVTYFSIPENKSNTEIFTMNVDGTNKIQITQTSVRENSAKWMKDGKHI
                     AFISSESGSSQLWMMDADGANRQQITQREGGINDFIFSPDESKILFIAEVKFGERASD
                     KYPDLPKATGIIVKDLMYKHWDEWIQTIPHPFIADFKNNKIVNEKDILEGEPYECPMK
                     PFGGIEQLNWSPDSKIIAYTCRKKTGKEYTLSTNSDIYFYYVESGKTENKTEGMMGYD
                     MNPKFSPNGKWIAWESMEREGYESDKSRLFVMNLQTGEKKDISSNFDQNCNNLSWSSN
                     SQNIYFVSCWHALTQIYKANIQNKQITPLTNGIHDYEFVIEAKNVLIGVKHSMSKPNE
                     IYSIDPKTGKETELSFENKEILDQLKMGKVEERWITTTDNKQMHTWVIYPPDFDPNKK
                     YPTLLYCEGGPQSPVSQFWSYRWNFQLMAANNYIIVAPNRRGVPGFGKEWLEQISGDY
                     GGQNMQDYLSAIDALAKESFVNKDKLGCVGASYGGFSVYWLAGHHNKRFKAFIAHDGM
                     FNLEQQYLETDEMWFVNWDFGGPYWDKNNSTAQRTYANSPHLFVDKWDTPILIIHGEK
                     DYRILASQSMAAFNVAILRDIPAEMLIFPDENHWVLQPQNGILWQRTFFEW"
     gene            8835..>9560
                     /locus_tag="P5236_00455"
     CDS             8835..>9560
                     /locus_tag="P5236_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HRU62870.1"
                     /translation="MSLLSKRMGEYKAPQEVQALGVYPYFRPIESEQDTVVKIHGKPV
                     LMFGSNSYLGLTNHPKLKEAAIKAIEKYGTGCAGSRFLNGTLDIHIELEERLAKFVNK
                     DAALVYSTGFSVNAGVIPCLTGRNDYLIFDERDHASIIEGKRLSFSKQFKYRHNDMES
                     LEDILKHCDYEKIKVIVLDGVFSMEGDVAKLPQIVELSKKYNASVYVDEAHTLGVFGK
                     TGAGICEHFGLTDDVDLIMGTFSK"
BASE COUNT         3120 a         1560 c         1617 g         3163 t
ORIGIN      
        1 atatgtagat ggtttcaata aaatagggtg gaatcattat aaaataataa attttcaata
       61 taaaaatcaa attgtttgca ctcgtgaaaa tcagtctgat agattattct tagatattag
      121 tgaagcaaat tgaatgatta ttcttagaaa attatgaatg gaagaactta tatagcattg
      181 aatttaggta gttcacagat ttcggcatta gcagcagaag ttttagacaa tggtgcagta
      241 agaattctgg gaagtgaatc taaaatttct gaggatgtga aacatggcat cattgaacat
      301 ccttctggat gtgcttttaa ggtcaatgaa ttactaaaat tattgcaaaa cagttcgaaa
      361 attcatgata tacaacaagt ttgtgttggt atcaatgcca gaagcatgaa agttatttct
      421 ggtcttgtaa ctcgtttcgt tggtcagaat aagatggtaa cagatatgtt gattaatgaa
      481 atgaaggatg aatgtgcaag aaaatattct caaaatgatt tgattgcctt tcaaacaata
      541 cctgttaaat atcaattaga tgaagaatat ataaccaatc ctgtagggaa gaaaggagta
      601 caaattacag gttattataa tgtagttatc ggaaataaat tgataaaaga acgttttgat
      661 ggttgtttcg atcgaactgg catcatggtt gaatattcac ctttagcaat agaatcattg
      721 gctactgttt taatggaaga agacgaacgc aaaaatggtt gtgctttgat tgattttgga
      781 gcaactacta ccactttggg agtttataaa aatgatatct tacaacaatt gttggtagta
      841 cctcttgggg gagaaaatat tacttccgat attgaagaat taggtataaa aaagtcgtat
      901 gcggaacgcc taaaatgttt aaaaggtgtg tcggtggtaa aatttgtaga aaaccctatt
      961 tacgtcgaga tgccttctga aaatgcggag gaaaatttgc gaatttcaac ctctttttta
     1021 gcaaacatta tcaatgctcg actggaagaa attacccaac caatttttaa aaccatcaat
     1081 caattgcctt tctctctgga aggaggaatt atcattactg gaggagcatc aaaattaaat
     1141 tatcttaagg aatttattga agaaaataca ggattgcctg ttcgttttgg gaatcattct
     1201 gaatggttag atgataaaac tccagagaaa ttttttgatg atacttattc tcaattaatc
     1261 ggaacaatcg tattaactga tgaatatcga aaattgaatc caacgaacgg acagtcaaga
     1321 aaagaaccaa aaatcaagcg aaattttaag gataaactta cagaacatat tataagtctt
     1381 tttgatgaag acagtgaaat gaaataagca aagtttttac ttgatttttt aaaatatata
     1441 aacagatgga agaacttatc gaaatgttac ctttagaatt acctttggtg aataattcta
     1501 ttataaaagt aattggtgtc ggtggaggtg gtggaaatgc agtaaatcac atgtaccggc
     1561 aaggcattac cgatgtatca tttgtaattt gcaacacaga taatcaagtc cttataaaat
     1621 cacccgtacc tataaaaata caattaggag ttaatactac tgctggactt ggagcaggag
     1681 gaaatcctga agttgctcgt caagcagcag aagaatctat tgataaaatc aaagaagttt
     1741 tgcaagataa taccaaaatg gtatttatta cagcaggcat gggtgggggt acaggcactg
     1801 gagcttcgcc tgtcattgcc aaagtagcgc atgatatggg aattcttaca gtaggaattg
     1861 ttaccattcc ttttgctttt gaaggaaatt taaaaattaa acaggcatta gaaggtgttg
     1921 cagctttgag tgaacatgta gatgctattc tggtaattaa caatgaaaaa ctaaaagata
     1981 tctatcctga tctggaatta tcaaatgctt ttgctaaagc cgatgatgtg ctgactaatg
     2041 cagctaaaag tattgctgaa attattaccg ttccgggcta tattaacact gattttgctg
     2101 atgtttatag tattatgaaa gatggtaaag tagccattat gaataccggg tatgcttcag
     2161 gtgaaaaccg aatcacgaaa gccatagaga atgctttaaa ttcacccttg cttaatacaa
     2221 atgatgtaaa tggtgcttcc aaagttttgt tgagtcttta ttgctcaact actaatcaga
     2281 ttaagatgga agaagtgcaa caaattcatg attttatgaa aaaagtaggg agaaatgtac
     2341 aggtaatttg gggtgctact ttcgatgaca ctttaggcga caacgttaaa attacgctta
     2401 ttgcgactgg ttatgatgtt agtgatattc caggtatgcc tacttcaatc gtaaataaac
     2461 ataaaaacag tcaatttgct gaaaaaaaat attcaactat tgatgaaaaa aaaccaccta
     2521 ttgctcattc tactgaagaa gaaaaggaat tacagattca aaaagcaatg gatgaatatt
     2581 atggtgatga tatttctcaa aatacttctg aaaaagaatt aaaagtggga atagaagata
     2641 atatacctgt agttaatttt aaagacctcg aagatttaga gaatgatgaa attttgaaaa
     2701 aagtagaaaa aattcctgca tggataagaa aacagaaaaa aaatgattaa cttcttattt
     2761 tgtttttact aaaaaattat acctatggaa actttatttg ataaaatttc agaagatatt
     2821 aaacaagcaa tgcttgctcg tgataaagta accctggaag ctttacgtgg cattaagaaa
     2881 gaatttcttg aggcaaaggc agcaaaagga aatgatggga cattgtcaga tgaagcagca
     2941 gtaaaaattc ttcagaaaat gatcaaacaa cggaaagaaa gtgctcaaat ttatgccgaa
     3001 cagaaccgac tcgaattagc tgaagctgaa ttggcagaag tagctgttat cgaaaaatat
     3061 ttaccccctc aaatggatga agaacagtta aaaactgaaa ttaataaaat cattgatgaa
     3121 gtaggagcca aaagtatgca agagatggga agagttatag gaattgcggt caaaaaactt
     3181 tctgggaaag ccgatagcca aatgatagct aacactgtaa aaagtttgct ggtaaagtaa
     3241 agtttgaaaa taaaaaagga attattcctg actttcaaag agtaattcct ttattaccga
     3301 taatgatttt tttacaatcc tttcactatt tcataagtat cagaagctat cataaattct
     3361 tcatctgttg gaatgacggc aacctttatg ctggaatcgg gtgtacttat caaggcttct
     3421 ttcccttgcg tggccatatt tattttttcg tcgattttaa ctcctaaata attaagatta
     3481 ttgcacacat ttgtacgtgt actggcttga ttttcaccaa caccacctgt aaatgccagt
     3541 aaatcaactc cattcaaagc agccgtataa gcaccaatat attttatgat gcggtattca
     3601 tacatttcca gggctaagcg ggctctttca tttccttttt caatagcagc tttaatatca
     3661 cgcatgtcag atgaaatgcc tgaaatacct aacattccgc tgtgcttatt aaacaaagta
     3721 gaaagtgtcg agggaccaat catctctttt tccattaaaa aagtagcaat tcccaaatca
     3781 acatcgcccg aacgagttcc catcattaaa ccttcaagcg gtgtaaatcc cattgatgta
     3841 tcaatagatt ttccattttt gatggcggta gctgaagctc catttcctaa atgcaccgtt
     3901 attatttttg tttgttgata atcaagtcct aaaaaatcgc aagctctttt tgaaacgtat
     3961 cgatgactcg ttccatgaaa gccatatcgc cgaatgctat attttgtata taaggaataa
     4021 ggaatagcat acatataggc atatttgggc attgtttgat gaaaagcggt atcaaaaatt
     4081 gccacctgtg gaacagaagg caataattct tctatagcgt gaataccagc taaattaggt
     4141 ggattatgta aaggtgctat atcaatgcat tcttcaattt ttcgaattac ttcatccgtt
     4201 atgagtacac ttttgttaaa tttttcacca ccatgaacaa cccgatgacc aacagcatca
     4261 atttcctcta aagatttaag tgttccatgg cgtttactgg taagtactcc taaaatatat
     4321 tcgattgcag tttgatgttc caaaacctca ccttctaaaa gaattttttg accatcatgc
     4381 cgcatatgtc tcaagcatga acctttcatt ccaatttttt ccacaatacc agagcctaaa
     4441 atctcattag tttctaaatt gaataattta tattttactg aggaactacc acagtttaat
     4501 actaatattt tcatgctttt tctcctcctt gtttgatgat atttccttct tcatcatatt
     4561 tataaaatcc ttttcctttt cttactccaa aatgtttcgc cctatataaa cgtaataaaa
     4621 caggtgaagg tttatatttg gaatttccat attcgttaaa taaattttcc attaaattga
     4681 caattttttc tatccccatt tgatcagcag tacgaaaaac acccagtcga tgcccaaatc
     4741 ctactgttaa aacacgatca atatcttcca ccgttgaaat tccttccatt aatatttcac
     4801 aggcttcatt taattcaata aggaataatc tgatacttac taaacctgct gattcagcaa
     4861 ctgccacata ttgatggttg attaaacgag caaattgaca aactttgtta taagtttcat
     4921 ctgaagtata aagtccacgt acaatttcta taatgtttga ttctggaatg ttggataaaa
     4981 aatgtaatcc tacacatctt tctttataac gtaattcaga tgccaattct gtaataacaa
     5041 cggtagatac attagaagca ataatagctt gaggtgataa cactttttcc aattgcaaaa
     5101 acacttcttt tctttccttt acatgtcttt caccagtttg gtcatcatat cgaacagctt
     5161 caattacaaa atcacagtca ctaaaatctt tgtaattttt tgtaccttgt acacgactta
     5221 aaactatttt tttttcattt tctgttaatc cccaattctc aattttttta ttcaattttt
     5281 gttcaattcg gctgaaagcg ttggcaattt tttcatctgt aggttctaaa aatactactt
     5341 cgattccatt aagaagtgct cttgtagcaa tagcactacc ttcataacca caaccaacta
     5401 ttcctatttt tgaaaaaaga gtgcgtcgtc ggcttctttt acttaaccca tatttttcaa
     5461 tgggttcgac aattatttca tgcataatat tttaaaaaat taaaagatca aaatccaatt
     5521 taataattca atttattttc ttaccaaatt tgacgttatg ataatcattt gtacaatatc
     5581 atttactgaa catcctcgag ataaatcgtt tacaggtgca gccattcctt gcagaatagg
     5641 accaatagct tgtgatcctg aaagacgttc tactaatttg tagccaatgt tactggcttg
     5701 caaatcagga aatattaaaa cattggctcg tcccgccact ggactatcgg gaacttttct
     5761 ctcagccaca gatggaacaa tagctgcatc agcttgtaat tctccatcga taagtaaatc
     5821 aggattcatt tcttttgcta ttttcgttgc ttttattact ttgtcaacta actcatgttg
     5881 agcacttcct ttggtcgaaa aactaagcat ggcaacacgt ggttcaattt ctgccaggtt
     5941 atgagcagtt tcggctgtac acattgctat ttgtgctaat tcttcggctg tgggacatgg
     6001 atttactgca caatctgcaa atattaataa accattttca ccatagtgag taggggtaaa
     6061 cattaaaaat aaacctgata caacatttat gccaggaaca gtttttataa tttgaaatgc
     6121 agggcgtaat acatgagtag taaaattgcg tgctccggct aattcgccat ctgcatctcc
     6181 attttttatc atcaaacaac caaaatataa aggatctttt gcaagtcgtg tggcttcttc
     6241 ataagttatc cctttatttt ttctaatttt ataaaggaga ttgatatatt cattcaattt
     6301 tggataattt tcaggatcaa ttatttttgc ctcggtaatg ctttttaaat gattttcttc
     6361 ggctaatttt cttatttttt cttctttccc aagtaaaatn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnc cattcaaaaa aggttcgttg ccataggata ccattttgcg
     6541 gttgcaatac ccaatggttt tcatctggaa aaatcaacat ttcagccgga atatctctta
     6601 atattgctac attaaaagca gccatagact gcgaagcaag gattctgtaa tctttttctc
     6661 catgaatgat taaaataggt gtatcccatt tgtctacaaa cagatgtggt gaattagcat
     6721 aagttcgttg ggcagtagaa ttattcttat cccagtaagg accaccaaaa tcccagttta
     6781 caaaccacat ttcatccgtt tcaaggtatt gttgttccaa gttaaacatc ccatcgtgag
     6841 cgataaaagc tttaaatcgt ttgttgtgat ggcctgccag ccagtaaacg gaaaatccgc
     6901 cataactggc tcccacacat cccaacttgt ctttgttgac aaaagattct tttgctaatg
     6961 catcgatagc tgaaagataa tcttgcatgt tttgtccgcc ataatcgccg ctgatttgtt
     7021 ccagccattc tttgccaaaa cctggaacac ctcttcgatt aggagcgaca ataatataat
     7081 tattggcagc cattaattga aaattccatc gatatgacca aaactgactg actggcgatt
     7141 gtggaccacc ttcacagtag agcaatgttg gatatttttt gtttggatcg aaatcaggtg
     7201 gataaataac ccatgtatgc atttgcttgt tgtccgtcgt tgtaatccat cgctcctcaa
     7261 cctttcccat ttttaattga tctagtattt ctttattttc aaaagaaagt tccgtttctt
     7321 ttcctgtttt tggatcaatg ctataaattt cattgggttt actcatggaa tgttttacgc
     7381 caataagaac gtttttagct tcgataacga attcataatc gtgaattcca ttagtaagag
     7441 gtgtaatttg tttattttga atgtttgctt tgtaaatttg agttaaagca tgccagcaac
     7501 ttacaaaata gatattttga ctattggaac tccaggataa attattgcaa ttttgatcga
     7561 aattgctgga aatatctttc ttttctcccg tctgtaaatt cattacaaac aaacgacttt
     7621 tatccgactc gtaaccttca cgttccatgc tttcccaagc tatccatttg ccgttgggtg
     7681 aaaatttagg attcatatca tatcccatca ttccttctgt tttattttca gtctttccac
     7741 tttccacata atagaaatag atatcagaat tagtggaaag cgtatattct ttacctgttt
     7801 ttttccgaca agtataggca atgattttac tatccggact ccaattgagt tgctcaatcc
     7861 caccaaaagg tttcatggga cattcataag gttctccttc cagaatatct ttctcgttta
     7921 cgattttatt gtttttgaaa tcagcaataa aaggatgtgg aatagtttgt atccattcgt
     7981 cccagtgttt atacatcaag tcttttacaa taatgcctgt ggctttcggc aaatcagggt
     8041 atttgtcgct ggcacgttca ccaaatttta cctcagcaat aaaaagaatt tttgattcgt
     8101 cgggtgaaaa aataaaatcg tttataccac cttctcgttg agtaatttgt tgacgattag
     8161 caccatcggc atccatcatc cacaactgtg aacttccgct ttctgaagaa ataaaagcta
     8221 tatgtttccc atctttcatc catttagcac tattttcacg aacagaagtt tgggtaattt
     8281 gtattttatt cgttccatcg acattcatag tgaaaatctc ggtgttgctt ttgttttcag
     8341 gaatgctaaa atatgtaaca tgataaagaa tttttgtctt atcaggagaa agttgaacgc
     8401 ccgatattct tccaaacgac caaagaactt caggagtcat tttcccattt tcaattttga
     8461 tggtagattt gccgatcaat tgattttttt cagaagcaag acaaatggtt gataaagtga
     8521 ccattgttaa aataaataat ttaaatattt gatgcatatt ttgttggaat ataaacttag
     8581 gttatattta tctttataag aataacaaaa gtaagatttt agactgttat attcaagcaa
     8641 tgttttttat tgaaataatt agtagaaatg aaagcgttag taatcaatag gataaatgtt
     8701 taatatctcc ttaaaaaact tgcttttttt gtagtagaag atagaaaatt gtatttaatt
     8761 tttttcaaca atccattatt ttttaaaaat gatgtatctt tgtgacgttt tttaataaat
     8821 aaaaacaaaa taatatgagt ttattaagta aaagaatggg ggaatataaa gcccctcagg
     8881 aagtgcaagc cttaggagta tatccttatt ttcgtcctat tgaatctgaa caggatactg
     8941 tagtcaaaat tcatggaaaa cctgttttaa tgtttggatc gaatagttat ttaggtttaa
     9001 ctaatcatcc taaattaaaa gaagcagcta tcaaagccat tgaaaaatat gggacaggtt
     9061 gtgcaggttc tcgttttctg aatgggacac tggatattca catcgaatta gaagaaaggt
     9121 tagcaaagtt tgtaaataaa gatgcggctc tggtttattc tacaggattt tccgtgaatg
     9181 ccggtgttat tccttgctta acaggcagaa atgattattt gatttttgat gagagagatc
     9241 atgcttccat tattgaagga aaaaggttat ctttttcaaa acagtttaag tatcgtcaca
     9301 atgatatgga gtcgcttgaa gatattttaa aacattgtga ttacgaaaaa attaaagtta
     9361 ttgttctcga tggtgttttc agtatggaag gtgatgttgc caaattgcct caaattgttg
     9421 aattatcaaa aaaatacaat gcctctgttt atgtggacga agcccatacg ttaggcgttt
     9481 ttggtaaaac aggtgcggga atctgtgaac atttcggctt aactgatgat gtagatttaa
     9541 ttatgggtac ttttagtaaa
//
LOCUS       DAOTKS010000010         9164 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_7717_length_9164_cov_5.303436, whole genome shotgun sequence.
ACCESSION   DAOTKS010000010 DAOTKS010000000
VERSION     DAOTKS010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9164
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7717_length_9164_cov_5.303436"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..456
                     /locus_tag="P5236_00460"
     CDS             46..456
                     /locus_tag="P5236_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HRU62871.1"
                     /translation="MIYQKEITLVPHSKGFHLITSEIINNLPPLPQEGLLHLFLKHTS
                     AALTINENADPDVQSDLKKIFDHLVPAHQPYYDHTLEGDDDMPAHAKSTLAGVSLLIP
                     ISNSRLNLGIWQGIYLCEFRQYGGKRKIVATIIS"
     gene            641..2113
                     /gene="proS"
                     /locus_tag="P5236_00465"
     CDS             641..2113
                     /gene="proS"
                     /locus_tag="P5236_00465"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRU62872.1"
                     /translation="MANSLTSRSENYSQWYNDLVVKADLAENSAVRGCMVIKPYGYAI
                     WEKIQSELDRRFKETGHVNAYFPLFIPKSFLSKEAEHFEGFAKECAVVTHHRLKTDPN
                     GNGLIVDPDAKLEEELIVRPTSETIIWNTYKNWIHSYRDLPLLINQWANVVRWEMRTR
                     LFLRTSEFLWQEGHTAHATEKEAIEEAQKILQLYTDFTEQFMSIPVIKGVKSANERFA
                     GAVETFCIEAMMQDGKALQMGTSHFLGQNFAKAFDVTFVDNNNQLQYVWATSWGVSTR
                     LMGALIMTHSDDNGLVLPPNLAPFQVIIVPIYKNEKQLADISSVADKIMTDLKKAGIS
                     VKYDNDDTKKPGWKFAEYELKGVPIRLAMGPGDIENHTVEIARRDTLTKETISMENVI
                     DEVSQQLNDIQKNLYERALTFRNANMKEINSYKEFKEKIEKGGFLLCHWDGTSETEEK
                     IKEETKATIRCIPFDLDKTPGKCMVTGKPSAQRVVFARAY"
     gene            complement(2384..3655)
                     /gene="serS"
                     /locus_tag="P5236_00470"
     CDS             complement(2384..3655)
                     /gene="serS"
                     /locus_tag="P5236_00470"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRU62873.1"
                     /translation="MLTLKYIIENTEDVIRRLEKKHFDAREIVSQVIELDNQRKKTQF
                     ESDQAAAEINKLSKEIGVFIKQGKQEEVNALKDRTIKLKESIKSLENQRNEIEKKLTA
                     LLVQLPNLPHESVPEGKGAEDNIIERVGGKMPVLPSDALTHWELAKKYDLIDFELGVK
                     ITGAGFPVYKGKGARLQRALIDFFLDSARGAGYLEIQPPYLVNAASAFATGQLPDKED
                     QMYYCPQDDLYLIPTAEVPVTNLYRGVILNESELPIKNTAYSACFRREAGSYGKDVRG
                     LNRLHQFDKVEIVQIQHPDKSYETLAEMVAYVESLVEKLELPWRTVRLCGGDMSFTSA
                     LTFDIEVFSAAQQRWLEVSSVSNFESYQANRLKCRFRTSDKKIQLCHTLNGSALALPR
                     IVAALLENNQTVDGIKIPAGLVPYTGFEMIY"
     gene            complement(3828..4091)
                     /gene="rpmA"
                     /locus_tag="P5236_00475"
     CDS             complement(3828..4091)
                     /gene="rpmA"
                     /locus_tag="P5236_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRU62874.1"
                     /translation="MAHKKGLGSSKNGRESESKRLGVKIFGGQLAKAGNIIVRQRGTV
                     HHPGENIGLGKDHTLYALVDGIVEFKKKKNNRSYVSIKPIEMN"
     gene            complement(4109..4423)
                     /gene="rplU"
                     /locus_tag="P5236_00480"
     CDS             complement(4109..4423)
                     /gene="rplU"
                     /locus_tag="P5236_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRU62875.1"
                     /translation="MYAIVEILGQQFKIEEGKKLFVHRMEEAERGSEIEFDKVLLIDK
                     DGDVTVGTPVVEGAKVKAEVLSHVRGDKIIVFHKKRRKGYKKHAGHRQNFTEILIKEI
                     VA"
     gene            complement(4553..5344)
                     /gene="cdaA"
                     /locus_tag="P5236_00485"
     CDS             complement(4553..5344)
                     /gene="cdaA"
                     /locus_tag="P5236_00485"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HRU62876.1"
                     /translation="MGFQIGIKDIIDIFLVALLLYEIFKLLKRSGGINIFIGILTFII
                     CWFFVSYVFKMQLLGDIMTWFVNVGAFALIVIFQEEIRRFFSRIGSRREVGFLTKIKH
                     AFNNSKIQQEQTDYDIVQIVLACRNMSKSLTGSLIVITRRNDLDFYAQSGEQIDSKIN
                     SRLIESIFFKNGPLHDGALIISHRKLRAAACILPISKNQTIPKRLGLRHRAALGITEI
                     SDAIAIVVSEENGHISWAINGELTLNVKPEQLEHFLSTELAEDME"
     gene            complement(5372..6205)
                     /gene="folP"
                     /locus_tag="P5236_00490"
     CDS             complement(5372..6205)
                     /gene="folP"
                     /locus_tag="P5236_00490"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRU62877.1"
                     /translation="MHLKLNQQLFDLSSPLIMGIINITPDSFYKGSRFTTGKAILHAV
                     DEILENGGSIIDIGGFSTRPSAEIISEEEEIYRLSKALEIILKKYPQTVISIDTFRAK
                     VARYIVENFQVAMINDVSGGTLDDQMFETIADLKVAYVLMHMRGTPQTMQQLTDYEDV
                     VTEVFQFLQKRLAQLRSLGVTDVIIDPGFGFAKTIQQNYQLLKKLSYFKELGAPILAG
                     LSRKSMIYKLLNITPDEALTGTIAVNMLALIGGASILRVHDVKEAVQTIKIFNEYIAA
                     L"
     gene            6462..7976
                     /locus_tag="P5236_00495"
     CDS             6462..7976
                     /locus_tag="P5236_00495"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRU62878.1"
                     /translation="MNVKTLIQFSALATILLLASCASQSKKENYSRVTGWKYNDEKGT
                     GFGVKKDFKTTVPPGMVSIEGGSFTIGEKGEVVLAPRNSRRRRITVSSFYMDQYEIRN
                     VDWREYTHWMETVFGKTAPKLVQKTYPDGKVWREELAYNEPYIEHYFTHPAFDQYPVV
                     GVTWEQVMDYCSWRTDRINELALINAGVIVPPDFASLKNLSFDSIIHNFIFNTQKYLK
                     QPTYQPKAGPKPKKDLYGQIRKADMSDGILLVSDIRLPTEAEWEYAAYALKADKNGFV
                     PAGKIYPWNGYGVRNPSKKELGQMMANFVRGRGDMMGTAGALNDRATITAPVDSYYPN
                     DFGLYNMAGNVNEWVLDVYRVTSFDDVSEYNSFRGNVYPTYIVEREDELGNNIYKIDS
                     LGRIATTVLKKDDVRNYKDGDPASKLSTDFVMYGADSTDIAAITNMTKIDPTDVLAPQ
                     ITEKTRVYKGGSWKDRVYWLNPSTRRYLDQDQCANDIGFRCAMSKIGSIEPVKY"
     gene            8137..>9164
                     /gene="asnS"
                     /locus_tag="P5236_00500"
     CDS             8137..>9164
                     /gene="asnS"
                     /locus_tag="P5236_00500"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HRU62879.1"
                     /translation="MKRTLVKEALKRNDFGAEVNIKGWVRTRRGNKNVSFIELNDGST
                     IHNLQIVVDNNKFGIDFLKPITTGACINVIGKLLPSMGKNQTIEIQTDKIEIYGTADP
                     ETYPLQKKEHTMEFLREIAHLRPRTNTFGAVFRIRHHMSIAIHTFFHERGFYYFHTPI
                     ITASDTEGAGQMFQVTTLNLNDIQKNEAGEVDYSYDFFGKQTGLTVSGQLEGELGALG
                     LGAIYTFGPTFRAENSNTPRHLAEFWMIEPEVAFNDINDNMILAQEFVQYCVRWALEN
                     CLDDLEFLNNRYDNDLIERLKFVVDNKFVHLSYTEGINILEEAIRNGHQFEFPVGWGL
                     DLQAEHER"
BASE COUNT         3183 a         1571 c         1483 g         2927 t
ORIGIN      
        1 taatcggatt caaaaaatgt ttttcattca tatatttaat caaaaatgat ttatcaaaaa
       61 gaaattacgc tggttcctca ttcaaaaggt tttcatttaa ttacttctga aatcatcaac
      121 aatttacctc cacttcctca ggaaggatta ttgcatttat tccttaaaca tacttctgct
      181 gctttaacta tcaatgaaaa tgccgatcca gatgtacaaa gtgatttgaa aaaaatcttt
      241 gatcatttag taccggcgca tcaaccctat tatgatcata ctttagaagg agacgatgat
      301 atgccagctc atgcaaaatc cacattagct ggagtaagtt tattaatccc catttcgaat
      361 agcagattaa atttaggtat ttggcaaggc atttatctct gtgaattcag acaatatggc
      421 gggaaacgca aaatagttgc tacgataatt agttaataca ctgtataaca gtttgctaac
      481 tttataaatc ccgctaaatg atttcttttg atttttttaa aaacagcttt tttcagagag
      541 tagaatcaag ccatttaaaa atattgctaa ctttgcactt cgaaaaacaa attttttatt
      601 gaataataat tttatttcaa ttagcagata acaacaaatt atggcaaatt ctttaacatc
      661 tcgttccgaa aattattctc agtggtataa tgatttagta gtaaaagctg atctggcaga
      721 aaattcagca gtgcggggtt gcatggtgat aaaaccctat ggatacgcta tctgggaaaa
      781 aattcaatca gagcttgatc gcagatttaa agaaacggga catgtaaatg cttattttcc
      841 actttttatt cctaaatctt ttttaagtaa agaagcagaa cactttgaag gatttgcaaa
      901 agaatgtgct gttgttaccc atcatcggtt gaaaaccgat ccaaacggaa atggtttaat
      961 tgtcgatcct gatgccaaat tggaagaaga attgattgta cgacctactt ctgaaacgat
     1021 tatctggaac acatataaaa attggataca ttcttatcga gatcttcctc ttcttattaa
     1081 tcagtgggcg aatgtagtaa gatgggaaat gcgtacccgc ctttttttgc gaacttcaga
     1141 atttctctgg caggaaggtc atacagccca tgcaactgaa aaagaagcta tagaagaagc
     1201 ccaaaaaata cttcaacttt atactgattt taccgaacaa tttatgtcta ttcctgtaat
     1261 taaaggagtg aaatcagcca atgaacgatt tgcgggtgca gtagaaacat tctgtattga
     1321 agctatgatg caagatggga aagctttaca aatggggact tcccattttt taggacagaa
     1381 ttttgctaag gcttttgatg taacttttgt agataacaat aatcaattgc aatacgtgtg
     1441 ggcaacttcg tggggagttt caaccagact aatgggagcc ttgattatga cacattccga
     1501 tgacaatggg ctggtgcttc cgccgaattt agctccattt caagtaatta ttgttcccat
     1561 ttataaaaat gagaaacaat tagctgacat ttcttctgtt gctgacaaaa taatgacaga
     1621 cttaaaaaaa gcaggtatca gcgtaaaata tgataatgac gacactaaaa aacctggttg
     1681 gaaatttgct gaatatgagt tgaaaggcgt tcccattcgt ttggcaatgg gacccggaga
     1741 cattgaaaat cacacagttg aaattgctcg acgtgataca ttaacaaaag aaactatttc
     1801 aatggaaaac gtaattgatg aagtttcaca acaactcaat gatattcaga aaaatcttta
     1861 cgagcgagca ttaactttcc gaaacgctaa tatgaaggaa ataaattcgt ataaggaatt
     1921 caaggaaaaa attgaaaaag gaggtttttt gctgtgccac tgggatggaa cttcggaaac
     1981 agaagaaaaa ataaaagaag aaacgaaagc caccattcgt tgcattcctt tcgatttaga
     2041 taaaacaccg ggcaaatgta tggtaacagg caaaccttcg gctcaaagag ttgtttttgc
     2101 aagagcttac taatgtttgt aacattaaat tattaatcac aagttacaag caaatcaaag
     2161 caaaataaag aaaataagaa aataagaaaa taatcagcaa taataactaa cgaaaagtat
     2221 aaacctattt taggacactc aaaactcaga actcaaaatt ccccactcga aactcctgac
     2281 catcaactaa attcagtaca tcggacctcg ttttcgaaac tcggaattta tagcttgtaa
     2341 tcccttctaa attatttttt agttttgaaa acgactcaca gtgttaataa atcatttcaa
     2401 atcccgtata aggaactagt ccagcaggaa tctttatacc atcaaccgtt tgattgtttt
     2461 ccagcaatgc agccacaatt cgaggtaaag ccaaagcact accattcaaa gtatgacaaa
     2521 gttgaatctt tttatcagaa gttctaaatc tacacttcaa acgatttgct tggtaacttt
     2581 caaaattaga aactgaactt acttccagcc agcgttgttg agcagccgaa aaaacttcaa
     2641 tatcgaaagt tagagccgaa gtaaaactca tatctcctcc acaaagacga actgttcgcc
     2701 acggtaactc caatttttcc accagcgatt caacataagc aaccatttca gcaagcgttt
     2761 cataagattt atcaggatgt tgaatctgaa caatttctac tttatcgaac tgatgaagac
     2821 gatttaagcc tcgaacatct tttccataag aaccagcttc ccggcgaaaa caagcagaat
     2881 atgcagtgtt cttaatgggc aactcacttt catttaaaat cacgccacga taaagatttg
     2941 tcacaggaac ttcggcagta ggaatcaaat acaaatcgtc ctgtggacaa taatacattt
     3001 gatcttcttt atcaggcaat tgaccggtag caaaagccga agcagcattg accagataag
     3061 gaggctgaat ttccagataa ccagctccac gagcagaatc caggaaaaaa tctatcaaag
     3121 ctcgttgtaa tcttgcacct ttccctttat aaacaggaaa acctgctcct gtaattttta
     3181 ctcctaattc aaaatcgatt aaatcatatt tttttgccaa ttcccaatgg gttaaggcat
     3241 ccgaaggcaa tactggcatt tttccaccaa ctcgctcaat gatattatct tctgctcctt
     3301 ttccttcagg aacactttca tgaggcaaat ttggaagttg aactaaaaga gcagttaatt
     3361 ttttttcaat ttcattccgc tgattttcca gagatttgat ggattctttt aacttaattg
     3421 ttctatcttt aagagcatta acttcctctt gtttaccttg ttttataaaa acaccaattt
     3481 cttttgatag tttatttatt tcagcagccg cctgatcact ttcaaactgt gttttttttc
     3541 gctgattatc aagttcaatt acttgagaaa caatttcacg agcatcaaaa tgtttctttt
     3601 ccaaccgtct aataacatct tcggtatttt caatgatata ttttaaagtg agcatattgt
     3661 caataaatta ttttaatgga tttatgttta cattcaacaa gatagatatt ttatgcaaaa
     3721 ataaaaataa ttttaaaagt taataaagaa aaaatctccc atcattttac tgacagtaaa
     3781 ataataggag atttttaagt ttttgtactt ttgcttaaaa aatgaaatta attcatttct
     3841 ataggcttaa tagaaacata agaacgattg ttttttttct ttttaaattc tacgattccg
     3901 tcaaccaaag catataaagt atggtctttg cctaaaccta tattttctcc cggatgatga
     3961 acagttccac gttggcgaac aataatattt ccagctttgg caagctgtcc gccaaatatt
     4021 ttaactccta atcgtttact ttccgattca cgaccgtttt ttgaactccc taaacctttt
     4081 ttgtgtgcca tttttttatc tatttgaatt aagcaacaat ttctttaatt aatatttccg
     4141 taaaattttg acgatgtcct gcatgttttt tgtaaccttt ccgacgcttt ttatgaaaaa
     4201 caattatttt atcaccccga acatgtgaca agacttctgc cttcactttt gctccctcta
     4261 ctacaggggt tccaacagta acatcgccat ctttatcaat aagcaatact ttgtcaaatt
     4321 caatttcaga acctctttct gcttcttcca tccgatgaac aaacaatttt ttcccttctt
     4381 caattttaaa ctgctgacct aaaatttcta caattgcata cattttcaat tctttttaaa
     4441 ttctatcggt aaggtgagat aaaatcttct taaaaaacct tatccaaaac gacctgcaaa
     4501 tttacaaatt tatttttgtt ttacaaaaat tatcctaaaa attttataaa atttattcca
     4561 tatcctcagc caactcagtt gataaaaaat gttctaattg ttcaggcttt acattcaatg
     4621 ttaattcacc atttatagcc catgaaatat gtccattttc ttcggaaacc acaatagcta
     4681 ttgcatccga gatttctgtt atccctaatg cagctctatg ccgtagtcct aatcgttttg
     4741 gaatggtttg atttttagaa ataggtaaaa tacaggcagc agctctcagt tttctatgac
     4801 taataatcaa tgccccatca tggagaggac catttttaaa aaaaatactt tcaataagtc
     4861 ttgaattaat ttttgaatca atttgctcac ccgattgagc ataaaaatcc aaatcattgc
     4921 gacgggttat cacaattaag cttcctgtta agcttttaga catatttctg catgctaata
     4981 caatttgaac aatatcataa tcagtttgtt cctgttgtat ctttgaatta ttaaaagcat
     5041 gcttaatctt ggtaagaaag ccaacttccc gtcgtgaacc tattcttgaa aaaaagcgac
     5101 gtatttcttc ctgaaaaatt acaattaacg caaaagcacc tacattgaca aaccaggtca
     5161 taatatcgcc cagcaattgc attttgaaaa cataagacac aaaaaaccaa caaataataa
     5221 atgtcaaaat acctataaaa atatttattc ctcctgagcg tttgagaagc ttaaaaatct
     5281 catagagcaa aagagcaact aaaaaaatat caataatatc cttaattcca atctgaaatc
     5341 ccataaatta tttaagtatt ttattatcag tttaaagagc agctatatat tcattaaaaa
     5401 tttttattgt ttgcaccgct tcttttacat catgtactcg caaaatagaa gctccgccaa
     5461 tcagagccag catgtttaca gcaatagttc ctgtcaaagc ttcgtcaggg gtaatgttca
     5521 atagtttata aatcattgat tttctggata aaccagctaa aataggagca ccgagttctt
     5581 taaaataaga caattttttt aataactgat aattttgttg aatcgtcttg gcaaaaccaa
     5641 aaccaggatc tattatcaca tctgttacgc ctaatgacct caattgagcc agacgctttt
     5701 gtaaaaactg aaaaacttca gtaacaacat cctcataatc tgtcagttgc tgcatagttt
     5761 gtggagtacc acgcatatgc attaacacat atgcaacttt taaatcggca atagtttcaa
     5821 acatttggtc atccaaagtt cctccactta catcattaat cattgcaact tgaaaatttt
     5881 caactatata tctggcaact tttgctctaa aagtatctat agatataaca gtttgaggat
     5941 atttttttaa aataatttcc aaagctttgg ataaacgata tatttcttct tcctcagata
     6001 ttatttcagc agaaggtctt gtagaaaatc ctcctatatc aataatactg ccaccatttt
     6061 ctaaaatttc atctacagca tgcaaaatag cttttcccgt agtaaagcga cttcctttat
     6121 aaaaagaatc aggagtaata ttaataatcc ccattataag gggcgaagaa agatcaaaaa
     6181 gctgctgatt caactttaag tgcataaaaa aataatttca ataaattctt gatcaaaata
     6241 attatcagaa gtgcaaaaat aaaaaattta aatgattgaa aataatggaa aataaaaaaa
     6301 tcaaaatatt aactatccat atatacgttt ttcagaaaaa atcagttatt attttgtaaa
     6361 ataaaatcaa tttatttgat aaaaaaacaa aaataaatta ataaaatact atatttgcaa
     6421 ggaattttta aaagtgagat tataaataaa gttcttttat tatgaatgta aaaaccttaa
     6481 tacaattttc tgctttggca actatcttat tattagcaag ttgtgcaagt caatctaaaa
     6541 aagaaaatta ttcacgagtt acagggtgga aatataatga tgaaaaagga acaggatttg
     6601 gagtaaaaaa agattttaag acgacagttc cccctggtat ggtttccatt gaaggaggtt
     6661 cgtttactat tggagaaaaa ggagaagtgg ttttagctcc aagaaatagt agacgacgca
     6721 gaataacggt aagttctttt tacatggacc agtatgaaat tcgtaatgta gattggcgtg
     6781 aatatactca ctggatggaa acggtttttg gaaaaacagc tcccaagctt gttcagaaaa
     6841 catatcctga tggtaaagtt tggcgagaag aattagctta caatgaacct tatatagaac
     6901 attatttcac tcatccagct tttgatcaat atcctgtggt tggagtaacc tgggaacaag
     6961 tcatggatta ctgtagctgg cgtacagacc gtattaatga attggcttta attaatgcag
     7021 gagtaattgt tccccccgat tttgcttcct taaaaaatct atcatttgat tccattattc
     7081 ataattttat ttttaatacc caaaaatatt taaaacaacc tacttatcaa ccaaaggctg
     7141 gtccaaaacc taaaaaagat ttatatggac aaatccggaa agcagacatg tctgatggta
     7201 ttttacttgt ctcggatata agactcccga ccgaagccga atgggaatat gcagcgtatg
     7261 cattaaaagc agacaaaaat ggctttgttc ctgcagggaa aatttatccc tggaatggtt
     7321 atggagttag aaatccatca aaaaaagagt tagggcaaat gatggcaaat tttgttcgtg
     7381 gacgtggcga tatgatggga acagcaggcg ctttaaatga cagagctact attacagcac
     7441 cggtagattc ttattatcct aatgatttcg gactttacaa catggctgga aatgtgaatg
     7501 aatgggtgtt ggatgtatac cgcgtcacaa gttttgatga tgtgagtgaa tacaactcgt
     7561 ttcggggtaa tgtttatcct acttacatag tggaaagaga agatgaattg ggaaacaata
     7621 tttataaaat tgactcgctc ggaagaatag caacaacggt tttgaaaaaa gatgatgtaa
     7681 gaaattataa agatggtgat cctgcttcaa aactttctac tgattttgta atgtatgggg
     7741 ctgattcaac tgatattgcc gccattacca atatgacaaa aattgatcca accgatgtcc
     7801 tggcaccaca aataacagaa aaaacaagag tatataaagg tggctcatgg aaagatcgtg
     7861 tttattggtt aaatccttct accagacgtt atctggatca agaccaatgt gctaatgata
     7921 ttggttttag atgtgctatg agtaaaattg gaagcataga accagtaaaa tactaaattt
     7981 aaaagaaaat gtttcttatt taattttttc tcttttttct ctgaattatt ttttattagc
     8041 acctgtacag caaagttcag gtgctgtttt gtaaatatcc ctcatatatc aattgaaatt
     8101 cgtaattttg caaattgaat aaataaagaa tttaagatga aaaggacact tgtcaaagaa
     8161 gcattaaaaa gaaatgattt tggagctgaa gtaaatatca aaggatgggt acgcactcga
     8221 cgagggaata aaaatgtgag ttttatagaa ttaaatgacg gttcaactat tcacaatcta
     8281 caaattgtgg tggataataa taagtttggt attgactttt taaagccaat tactactgga
     8341 gcttgcatta atgtaattgg aaaactttta ccttcaatgg gtaaaaatca aacaatagaa
     8401 attcaaacag ataaaattga aatttatgga actgctgatc cagaaactta tcctcttcag
     8461 aagaaagaac atactatgga atttttacgt gaaattgctc acttgcgtcc ccgtactaat
     8521 acttttggag cagtttttcg cattcgtcat cacatgtcaa tagccattca tacatttttt
     8581 cacgaaagag gtttttatta ttttcatact cccattatca ctgcttctga tacagaaggt
     8641 gccggacaaa tgtttcaagt taccacttta aacttgaatg acatacaaaa aaatgaagca
     8701 ggagaagtcg attattctta tgacttcttt ggcaagcaaa caggattaac tgtctcgggg
     8761 caacttgaag gagaattagg agcattgggc ttgggtgcta tttatacttt tggacccact
     8821 tttagagccg aaaattcaaa tacacctcga catttggctg aattctggat gatcgagcca
     8881 gaagttgctt tcaatgatat taatgataat atgatactgg ctcaggaatt tgttcaatac
     8941 tgcgtccgtt gggcattaga aaattgtttg gatgatctgg aatttttaaa taatcgttat
     9001 gataatgatt taattgaacg attgaagttt gtagtggata ataaatttgt tcatttaagt
     9061 tatactgaag ggattaatat tttggaagaa gctattagaa atgggcatca atttgaattt
     9121 ccagtaggtt ggggattgga tttacaggca gaacatgaaa gata
//
LOCUS       DAOTKS010000011         8558 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_8347_length_8558_cov_4.224627, whole genome shotgun sequence.
ACCESSION   DAOTKS010000011 DAOTKS010000000
VERSION     DAOTKS010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8558
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8347_length_8558_cov_4.224627"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..1175
                     /gene="dprA"
                     /locus_tag="P5236_00505"
     CDS             60..1175
                     /gene="dprA"
                     /locus_tag="P5236_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRU62880.1"
                     /translation="MNNDTLKYKIALTLIKGIGCNLAKNLLAYVGSAEGVFHEKEKNL
                     AKIPGIGTILSKEIVSQNVLDRAEAEIDFIEKNHIQPLFFTDKEYPFRLKECNDAPIM
                     IYCKGECNLNDGKFLGVVGTRKATEEGKRNCRQLIEELSSAIPDLIVVSGLAYGIDIC
                     AHKTALANKTSTIAVLGHGLDRIYPASHRSIAVKILKNGCLLTEYLTNTNPDRQNFVR
                     RNRIIAGLCDAVVVVESAIKGGALITAELANDYDRDVFAFPGRVTDERSAGCNALIKN
                     NKASLIESAEDVLQFMNWDKQNINSQGNIQKTMYFDLSYEEQEILSLLRHSHDGIQVN
                     ELAVSLSQPFSKISSLLLEMEFKGLVVCLPGNLYKAI"
     gene            complement(1242..2309)
                     /locus_tag="P5236_00510"
     CDS             complement(1242..2309)
                     /locus_tag="P5236_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRU62881.1"
                     /translation="MFLEKTIFGNSLINWGISLLILLGAFLLNKGIVLLNRKVIFKIT
                     GKTKNKLDDILFRSLESPILLAIALLAIWIAFRRLDLSATVDKIFDKAYQFLIVINIT
                     WFVAKLVNALIKEYLLPATKEGSGNKRLDESMIMIIRRLSVGIVWTIGIIMALNNTGV
                     NVGALLASLGIGGLAVALAAQDTLKNFFGGLTIFMDHPFRIGDRIIVNGVDGNVEDVG
                     IRSTKIRTFDRRIVTIPNYKIVDASIENVTIEPMRRVVMKIGLTYDTAPNKMEEALTI
                     LKNMPQKLTEIDDKEVNAYFTDFSDSALIITYIYFIKKKSDIMLTISKVNIEILEQFN
                     AAGLNFAFPTRTIYIENQENG"
     gene            complement(2353..4254)
                     /locus_tag="P5236_00515"
     CDS             complement(2353..4254)
                     /locus_tag="P5236_00515"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HRU62882.1"
                     /translation="MNHGFIRVAAAVPEVRVADCAFNFSSIANLIQQAEKEYVQVICF
                     PELCLTGYTCGDLFFQKQLLEDAEKSLNDLQLLTASTKAIVIVGMPLRCESRLLNAAV
                     VLQQGKILGIVPKTFLPNYNEFYEKRWFTSAMDIPSTNIFIRGQEIPLGTDLLFSDGN
                     FTFGIEICEDVWSPLPPSTQLCLQGAEIIFNLSASNELVEKHAYRLQLIQQQSARCHA
                     GYVYASAGEGESTTDLVFTGPAIIAENGKILESSKRFTFEPQLIVTEIDVECLQSERM
                     RNNYFHERTTLLTPRKVMIEPTSFPIEELKRMVEPYPFIPSLEKNEESCREIFSIQMA
                     GLAKRWLHTKSQHLLVGISGGLDSTLALLVCVKTADQLHFDRKRIMGITMPGFGTSEQ
                     TYTNAYRLMKSLGITILEIPIKDACLQHFKDIGHDPAVHDTTYENAQARERTQILMDL
                     ANKYQGLVVGTGNLSELALGWTTYNGDHMSMYAVNSGIPKTLVRCLVEWTAKQLDENS
                     KKILKDILQTPVSPELLPAKENGEIAQKTEEVIGPYGLHDFFLYYFIRFGFSPNKILF
                     LAKNAFKDQHSEETLRKWLKVFLRRFFSQQYKRSCMPDGPKVGSVNLSPRGDWRMPSD
                     VNFPWEELL"
     gene            complement(4358..5236)
                     /locus_tag="P5236_00520"
     CDS             complement(4358..5236)
                     /locus_tag="P5236_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HRU62883.1"
                     /translation="MSRKKNNSVSHFKNVHFTSIFSMSLVLFLVGLVCLLLFTARDLS
                     IRVRENINLSIILDNGATNSEIVRIKNYLNAAPFAKSVKYISKDDALKELIESLGEDP
                     QEFLGYNPLLASFEVKLHANYANNDSVAKIESKLKTFPHINRVVYQKDVVDLVNENIK
                     KLSYILLGLTVILLLISLALINSTIRLLIYSNRFLINTMKLVGATSWFIRWPYVRRSI
                     VNGIIAAFLALIYLAGTIAYVQYEFGFKGMFIPAVTAVSVTAIILFLGILLSGVSSYF
                     AVGRYLRMPTDDMYFV"
     gene            complement(5296..7773)
                     /locus_tag="P5236_00525"
     CDS             complement(5296..7773)
                     /locus_tag="P5236_00525"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HRU62884.1"
                     /translation="MQTIMPHVVAVILFLIISFWYFSPVLEGKQLIGHDTKSWMCMAK
                     ETIDYNKTHKDKTLWTNSMFSGMPTYQISMTQPNNFSKYVDQFFRLFPAPVFYLILYL
                     LGAYVLLLAFGLNPWLAIIGSFAFSFLSYNFIIIAVGHNSKAITLAYIAPLIGSVYLA
                     FRSKPLLGSLLTAFFASLAIYANHIQILYYALLIIIILGIVEFVYSIKEKQLVSFFKT
                     VGLLLVAAAIGIGMNATSLLTTYEYSQYTMRGKSTGLTMEGQNSQHGLSKDYITQWSY
                     GIDETLTLLIPNFKGGASVGTLSPKSETAKKLQSYGVPNVERIMKDMELPLYWGTQPF
                     TSGPVYAGAIVCFLFVLGIFLVDKRTKWWLLLMIILTVMLSWGKNFMPLTDFFINYVP
                     FYNKFRTVSMILVATTFGMALMAALALKEVFNPNAEAKEVIKAVKISAIIVGGIALLF
                     ALFPSLAGDFVSPTDQQFQGDYAFLKETLPIDRAALLRSDAFRSLAFVVLAAFMIWLY
                     MKKYLKKNVAYVLFGFFILMDMVPIAKRYLNEKNFEKKQRIENLILPSTADKFILADK
                     SYYRVVDATVNIFNDATPSYFHKNIGGYHAAKLRRYQDLIDIHLTKELGQLFSAFGRV
                     NSVEQIVSTFDSLQVLNMLNLKYLIFNKEYQPIINPYANGNAWFVQKIHLAPNADEEM
                     RLLGNIDTKTELVADQSYASLLPTAIQPDSTARIELIHYAPNHLIYSFNSNTDQVAIF
                     SEIYYDKGWNAYINGQKVPYIRANYLLRAMPLKAGMYNIDFKFEPRSYAVGNVIALIS
                     SIILILCIASFIFYSVKIEKQTEKSKS"
     gene            complement(8254..>8558)
                     /locus_tag="P5236_00530"
     CDS             complement(8254..>8558)
                     /locus_tag="P5236_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRU62885.1"
                     /translation="SAGITMVTAMLSAFTKRKVRKNIAMTGEITLRGKVLPVGGIKEK
                     ILAAKRAGITDIILSVENQKDIDEIKPNYLKGLHFHYVSDIMEVIDFALLKEKVIS"
BASE COUNT         2900 a         1559 c         1446 g         2653 t
ORIGIN      
        1 aataagctta ttgctgtttc ttatatttta tgttttaact aaaaaaaaat tcaagaaaag
       61 tgaataatga taccctaaaa tataaaattg ctcttacttt aataaaagga ataggatgta
      121 atctggcaaa aaatctactg gcttatgtag gtagtgccga aggcgttttt cacgaaaagg
      181 aaaaaaatct tgctaaaatt ccaggtattg gtactatttt atccaaagaa attgtctctc
      241 aaaatgtctt agacagagct gaagctgaaa tagatttcat tgaaaagaat cacattcaac
      301 cactattttt tacagataaa gaatacccct ttcggctaaa agaatgtaat gatgctccta
      361 taatgattta ctgcaagggc gaatgcaact taaacgacgg aaaattttta ggtgtggtag
      421 gtactcgtaa agctacggaa gaaggaaaaa gaaattgccg acaacttatt gaggaacttt
      481 ccagtgccat tcctgactta atagtggtaa gtggtttggc ttatggcatt gatatttgtg
      541 cacataaaac tgctttagca aataaaacat ccacgattgc cgttttaggt cacggtttgg
      601 atagaattta tccggcatcg caccgctcga tagcagtaaa aattttgaaa aacggttgtt
      661 tactgacaga atatttaact aatacaaatc ctgatcgaca aaattttgtg cgaagaaatc
      721 gcatcattgc aggactttgc gatgcagtgg ttgtcgtgga atcggctata aaaggtggtg
      781 cgcttattac agccgaactt gctaacgatt atgatcgaga tgtatttgct tttcctggac
      841 gagtaacaga tgaacgttct gccggttgca atgctttaat taaaaataat aaggcttcct
      901 tgattgaatc agcggaagat gttttgcagt ttatgaattg ggataaacag aatataaata
      961 gtcagggtaa tattcagaaa accatgtatt ttgatctttc ttacgaggaa caagaaattc
     1021 tttccttgct gagacattct catgatggta ttcaggtaaa tgaattggcg gtttcgttgt
     1081 cccaaccttt tagcaaaatt tcttcactct tgcttgaaat ggaattcaaa gggctggtag
     1141 tttgtcttcc tggaaatctt tataaagcaa tttgatggaa aattcttctt ttgacgggaa
     1201 tttttaagtt caaaaattgt ttttgaatgt agataaatag attatccgtt ttcttgattt
     1261 tcgatataaa tagttctggt ggggaaagca aaattcaatc ctgctgcatt aaattgttct
     1321 aatatttcta tatttacctt cgaaatagta agcataatat cacttttttt cttaataaaa
     1381 taaatatagg taataatgag ggcggaatcg gaaaaatctg taaaataagc atttacttcc
     1441 ttatcatcga tttctgtcaa tttttgaggc atatttttta aaatagtaag agcttcttcc
     1501 attttattag gggcagtatc ataagttaag ccaattttca ttactactcg tcgcataggt
     1561 tcaatagtaa cattttcaat agaagcgtcc acaattttat agttgggaat agtaacgatc
     1621 ctacgatcga aagttcggat tttagtactg cgaataccaa catcctctac attaccatct
     1681 actccattta cgatgatacg atccccaata cgaaaaggat gatccataaa aatagtcaat
     1741 ccaccaaaaa aattttttag agtatcctgg gcagctagag caacagctaa accaccaatt
     1801 cccaagctgg caagaagagc acccacattt acaccggtat tatttagtgc cataatgata
     1861 ccaatagtcc aaacaattcc caccgagaga cggcgtataa tcataatcat gctctcatct
     1921 aaacgcttat tacctgaccc ttccttggta gcaggaagca aatattcttt tatgagggca
     1981 ttaaccagtt ttgcaacaaa ccacgtaatg ttgatgacaa tcagaaattg ataggcttta
     2041 tcaaaaatct tatccacggt agcactcaaa tctaatcggc ggaaagcaat ccagatagcc
     2101 aataaagcta ttgccaataa aataggggat tccagcgatc tgaataaaat atcatccagt
     2161 ttatttttcg tcttgcctgt aattttaaaa attacttttc gatttaataa tacaattccc
     2221 ttgttcaaaa gaaaagctcc tagcaggata agcaaagaaa ttccccaatt tataagtgaa
     2281 ttcccaaaaa ttgttttttc aagaaacata ttttattaaa ttttttttat ttaaaatcaa
     2341 ttttatttga actcataaaa gttcctccca tggaaaattg acatcactgg gcattcgcca
     2401 gtcacctcgg ggtgaaagat taacagaacc tactttggga ccatcgggca tacaagaacg
     2461 tttatattgc tgagaaaaaa aacgacgcaa aaatactttt aaccatttcc gcaatgtttc
     2521 ttctgaatgt tgatctttaa aggcattttt ggctaaaaaa agaattttat tgggagaaaa
     2581 accaaaacgt ataaaataat acaaaaagaa atcgtgtaat ccatagggac cgataacttc
     2641 ttcggttttt tgagcaattt caccattttc cttagcgggt agtaattcgg gacttacagg
     2701 tgtttgaaga atatctttca gaattttttt tgaattttcg tccagttgct tagcagtcca
     2761 ctcaaccaga caacgtacta atgtctttgg gattccgcta ttcactgcat acatcgacat
     2821 gtgatcgcca ttgtaagtag tccaacccaa agctaattct gataaatttc ctgtgccaac
     2881 taccaaacct tgatatttgt ttgccaaatc catcaatatc tgcgtacgtt cacgagcttg
     2941 agcattttcg taagttgtat cgtgaacagc aggatcatgt ccaatatctt taaaatgttg
     3001 caaacaagca tctttaatgg gaatttccag aattgtgatt cctaaagatt tcattaaacg
     3061 gtaagcgttg gtataagttt gttctgaagt tccaaatcct ggcatcgtta tacccataat
     3121 gcgttttcgg tcaaaatgca attgatcggc tgtttttacg caaaccaaca aagcaagtgt
     3181 agaatccagt ccgccagaaa ttcctaccag caaatgttgt gattttgtat gcagccagcg
     3241 tttcgccaaa cctgccattt gaatggaaaa aatttcccga cagctttcct cgtttttttc
     3301 tagtgatgga ataaaaggat aaggttcaac cattcttttt agttcttcga ttgggaaaga
     3361 agttggctca atcattactt ttcttggggt aagcaatgtc gttcgttcat gaaaatagtt
     3421 atttcgcatt cgttcacttt gcaaacattc tacatcgatt tctgttacta tcagttgtgg
     3481 ctcaaaggta aatcgtttag aactctctaa aatttttcca ttttcggcta tgatggcagg
     3541 tcctgtaaaa accagatcgg tagttgattc accttcacca gccgaagcgt aaacataacc
     3601 tgcatgacag cgtgctgatt gttgctgaat aagttgaaga cgataagcat gtttttcaac
     3661 gagttcgtta cttgcagaaa gattgaaaat aatttcagct ccttgcaaac acaattgtgt
     3721 gcttggcgga agaggcgacc atacatcttc acaaatttca attccgaaag taaagttgcc
     3781 atccgaaaat aaaagatcag ttcctaaagg aatttcttgc ccacgaataa aaatattagt
     3841 agaaggtata tccattgcag aagtaaacca gcgtttttcg taaaactcat tataattggg
     3901 taaaaaagtt tttggtacga ttcccagtat tttcccttgc tgtaatacga cagctgcatt
     3961 caaaagtcga ctttcacaac gcaatggcat acctacaatt actattgcct ttgttgaggc
     4021 agtgagtaat tgcaaatcat tcaatgattt ttctgcatct tccagcaatt gcttttgaaa
     4081 aaaaagatct ccacaagtat aaccagtgag acataattca gggaaacaaa taacctgcac
     4141 gtattctttt tctgcctgct ggattaagtt agcaatgctg gaaaaattaa aagcacaatc
     4201 tgccactctg acttcaggaa cagcagcagc cacgcggata aaaccatgat tcatattgag
     4261 cttgtttttt aatttttatc caataaaaaa taagaaatac ttaaaaaatt aatattcact
     4321 ttgctgaaaa atctcttaaa tataaggact aaaaaaatta cacaaaatac atatcatccg
     4381 taggcattcg caaataacgt cccacagcaa agtaagaaga aacgccgctg agcaatatac
     4441 ccagaaacaa aataatagct gtgacagaaa cagccgtaac cgctggaatg aacattcctt
     4501 taaagccaaa ttcgtattgc acataagcaa ttgttccagc cagataaatc aatgccaaaa
     4561 aagctgctat aattccatta acgatgcttc gtctgacgta aggccagcga ataaaccacg
     4621 aagttgcccc taccagtttc attgtattta ttaaaaagcg attggaataa atcagtaaac
     4681 gaatggtact gttgataagc gccagtgaaa taagtaataa aataactgtc agccccagca
     4741 aaatataact tagttttttt atattttcat tgaccagatc cactacatct ttctgataaa
     4801 ccacacggtt gatgtgcgga aaagttttta atttcgattc aatttttgct acactgtcat
     4861 tgttggcata attggcatgt aattttactt cgaacgatgc cagcaaaggg ttgtaaccca
     4921 aaaattcctg cggatcttcg cccaaacttt caataagttc tttcagagca tcatcttttg
     4981 aaatatattt tacggatttt gcaaaaggag ccgcattcaa ataattttta atccttacta
     5041 tttcactatt ggtggcacca ttgtccaaaa taatagataa attaatattt tctcttaccc
     5101 gaatgctcaa gtcacgagct gtaaaaagca acagacacac cagtcctacc agaaataata
     5161 ccaacgacat gctaaaaatc gaagtaaaat gaacattttt aaaatgagaa acggaattat
     5221 ttttctttct tgacatttta ttatttatct ttttttaatc aaaatcataa ataaattaga
     5281 aagtttctaa aattatcaac ttttgctttt ctctgtttgt ttttcaattt ttaccgaata
     5341 aaaaataaaa cttgctatac ataaaatcaa tattattgaa gaaataagag caattacatt
     5401 ccctactgca tagctacggg gttcaaactt gaaatcaatg ttatacatac ctgctttgag
     5461 tggcatggct cgtaataaat aattagcccg tatatacggt actttttgac cattgatgta
     5521 agcattccac cctttatcgt agtaaatttc tgaaaatatt gctacctgat cagtattgga
     5581 gttgaaactg taaatcagat ggttaggcgc gtaatgaatc agttcaatcc ttgctgtact
     5641 atctggctga atagcagtgg gcagtagcga agcataagat tgatcagcta ccagttcggt
     5701 tttggtatca atgttaccta ataaacgcat ctcttcatcc gcattcggtg ccaaatgaat
     5761 tttctgtaca aaccacgcat tgccattagc ataaggattg ataataggct gatactcttt
     5821 attgaaaatt aaatatttta aattcaacat attcaggact tgcaaagaat caaatgtgga
     5881 aacaatttgc tccactgaat ttactcttcc aaaagcagaa aaaagttgac ccaattcttt
     5941 cgtcagatga atatcaatca aatcttgata acggcgtaat tttgctgcat gataaccgcc
     6001 aatattctta tgaaaataag aaggggtggc gtcattaaaa atattcacag tagcgtccac
     6061 tactctataa taagatttat ccgctaaaat gaatttatcc gctgttgaag gaagtatcaa
     6121 attttcaatt cgttgtttct tctcaaaatt tttctcattt aaataacgtt tggcaatggg
     6181 caccatatcc atcagaatga aaaagccaaa caagacataa gctacattct ttttgagata
     6241 ttttttcata tatagccaga tcataaaagc tgccaaaacc acaaaagcaa gtgaacggaa
     6301 ggcatctgaa cgaagtaaag cagcacgatc gattggcagt gtttctttca agaaagcata
     6361 atcgccttga aattgctgat ctgtgggaga aacaaaatcg ccagccagcg aaggaaacaa
     6421 agcaaatagt aacgcgattc cacctactat aatagcacta atttttacgg ctttaattac
     6481 ctctttagcc tctgcattgg gattgaaaac ttctttcagt gcgagggctg ccattaaagc
     6541 cattccaaag gttgtggcaa ccaaaatcat tgaaacagtg cggaatttat tataaaaagg
     6601 aacataatta ataaaaaagt cagtcagcgg cataaaattt tttccccatg aaagcattac
     6661 ggtgagaata atcatcagta aaagccacca ttttgttcgt ttatctacga gaaatattcc
     6721 caatacaaac aagaaacaga caattgctcc tgcataaaca ggaccagaag taaaaggttg
     6781 tgtgccccaa taaagcggca actccatgtc tttcataatt ctttcaacgt ttggaacacc
     6841 atagctttgc aacttttttg ctgtttcact tttgggcgaa agtgttccta cactggctcc
     6901 ccctttaaaa ttaggaatta ataaagtgag agtttcatca atgccgtaac tccattgggt
     6961 aatataatcc ttactcaagc catgctgaga attttgtcct tccatagtta atcctgtgga
     7021 ttttcctcgc atggtatatt gactatattc ataagtggtg agcaaactgg tagcattcat
     7081 tcctatccca atagcagctg caacaagcaa taagccaact gttttaaaaa atgaaacaag
     7141 ctgtttttct ttgatgctgt aaacaaattc cacaatgccc agtataataa taattaaaag
     7201 agcataatac aaaatttgaa tatgattagc ataaatagct aaactggcga aaaaagccgt
     7261 taataaactt cccagcaaag gtttggaacg aaaagccaga taaacgctac caatgagtgg
     7321 ggcaatataa gccagtgtaa tggcttttga attatgacct acagcaataa ttataaaatt
     7381 gtaggaaagg aaagaaaaag caaacgagcc aataatggca agccaggggt tcaaaccaaa
     7441 tgctaataaa agaacataag cacccaataa atatagaatt agataaaaaa caggggcagg
     7501 aaataatctg aagaattgat ccacatactt gctaaaatta tttggctgtg tcatggaaat
     7561 ttgataggtg ggcatgccac taaacataga gttggtccaa agcgttttat ctttatgagt
     7621 tttgttatag tcaattgttt cttttgccat gcacatccag cttttggtgt catgaccaat
     7681 caattgtttt ccctccaata cagggctgaa ataccaaaaa gaaattatga gaaacaaaat
     7741 cacagccacc acatgcggca taatggtttg catcgtttta ttcatcgcag tgagaaataa
     7801 ataatttaaa attgtaaatt gataagaact ttacaaaaat acattatttt ttggaaaagc
     7861 tacctaatta agaataaaaa tcgaaaacaa tgaggtatgc gttataaggt accaagattt
     7921 agttataatc ggtggtttgg aatttaaagt gtggaattag tgagttgaaa gtttgtgagg
     7981 attatcgtgt gggtcaggac tttgcgagtt ttcaagttag taattttgag cagattcata
     8041 taaatgttta aaaaatgttg gctgttttta agcatttacc tttcgcaatt cataactcac
     8101 gaacttaaaa caacaaacta ttgattatca gctaaaaatt gtatcttgcc caacagaacg
     8161 taaaacttgt gaatttgcat ctcgttttct tgtattccgc aactcgcact tcacaaactc
     8221 atacctccca cctcgaaaat tcctaacttt tcattaagaa ataactttct ctttcaacaa
     8281 agcaaaatca attacctcca taatatcact cacataatga aaatgaagac ctttcagata
     8341 attgggtttt atttcatcta tatctttttg attttcaaca cttaaaataa tgtctgttat
     8401 accagctcgt ttagcagcca gaattttttc cttgataccg ccaacaggaa gaactttgcc
     8461 tcgcaaggta atttcacccg tcatggcaat attttttctt acttttcttt ttgtaaatgc
     8521 tgaaagcata gccgttacca ttgtaattcc tgctgaag
//
LOCUS       DAOTKS010000012         8337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_8593_length_8337_cov_4.605530, whole genome shotgun sequence.
ACCESSION   DAOTKS010000012 DAOTKS010000000
VERSION     DAOTKS010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8337
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8593_length_8337_cov_4.605530"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="P5236_00535"
     CDS             <1..458
                     /locus_tag="P5236_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRU62886.1"
                     /translation="SPIRRFFLNLTMGDAFLSDDLAQETFIKAFLSLSTFKALSDFST
                     WLFRIAYNVYYDAVRTRKDFYDLEEKEINQQYQVNNSYSSDKEDFYKALINLNKMEKT
                     AVLLYYMEDKTQREIATIMKCPIGSVKNYIRKGKEKIANFLKENGYGNE"
     gene            445..756
                     /locus_tag="P5236_00540"
     CDS             445..756
                     /locus_tag="P5236_00540"
                     /inference="COORDINATES: protein motif:HMM:NF027794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5056 domain-containing protein"
                     /protein_id="HRU62887.1"
                     /translation="METNETDKNFQDFFKLHKHDIADNGFASRTLRHLPQRKDNSWIV
                     WLFTTIGIATALYIGIFSDWLMNFIEHMFNLPFIYLLIGVALFPLVIGLCIMFWEKEQ
                     Y"
     gene            complement(853..1200)
                     /gene="rplS"
                     /locus_tag="P5236_00545"
     CDS             complement(853..1200)
                     /gene="rplS"
                     /locus_tag="P5236_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRU62888.1"
                     /translation="MDLLKVAEQAFQVEKSFPEFKSGDTITVAYRIKEGNKERIQEFR
                     GVVISIKGQEGKKRFTVRKMSGNVGVERIFPMDSPFIESITVNKRGKVRRAKLFYLRK
                     LTGKKARIKEKRY"
     gene            1655..3469
                     /gene="typA"
                     /locus_tag="P5236_00550"
     CDS             1655..3469
                     /gene="typA"
                     /locus_tag="P5236_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRU62889.1"
                     /translation="MQKIRNVAIIAHVDHGKTTLVDKMLLAGHLFRDNEKVGELIMDN
                     NEIERERGITILAKNASINYNGYKINIIDTPGHADFGGEVERVLNMADGVLLLVDAFE
                     GPMPQTRFVLQKALQLGLIPIVVINKVDKPNCRPDEVQEEVFDLMYHLNATEEQLNFH
                     TIFGSAKQNWMSEDWRKPTNNIFPLLDAIIKYIPEPFYLEGTPQMLITSLDYSPYVGR
                     IAVGRVHRGTLYEGMDVSLVKRDGFVVKTKIKELYTFIGLERKRISEVKSGDICALIG
                     IDNFEIGDSIADINQPEPLKPIAVDEPTMSMVFTINNSPFYGQEGKYITSRHLHERLM
                     RELDKDLALRVKKEPDKDIWHVYGRGVLHLSILIETMRREGYELQVGQPQVIFKMING
                     IRCEPVEQLIINLPEEYSGRIIEIIASRKGEMLKMETMNDRIQLEFIIPSRGIIGLRN
                     QVLTASGGEAIMSHRFLEYQPYKGPIEKRTNGSLIAMETGTAFAYALDKLQDRGRFFI
                     SPQEEVYAGQVVGESSKEGDIVVNVTKSKKLTNMRASGSDEKIKLTPPVIFSLEEALE
                     YIKEDEYVEITPSNIRLRKIYLDENERKRMSKNVSATA"
     gene            3529..4161
                     /locus_tag="P5236_00555"
     CDS             3529..4161
                     /locus_tag="P5236_00555"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRU62890.1"
                     /translation="MKKEFWVVLFLLLIFSSKILAQFGVKIGINEANEVITSSNLSTP
                     IEKPTLQNLTAYHIGVIYQYTSKIGLGFDIGTSLSQKGCFLQSDSICDIYRELNYIEI
                     PFNIRYRLSWGIIGIYGFGGLYGAYLLKGQKITETNTSSIPEKFSYTNFNDRTDYGYT
                     FGGGIEIFKKIQLEANWNKGLKENIYYTNSDTQIFTTKNNVFSFNLTFIF"
     gene            4313..5671
                     /gene="tig"
                     /locus_tag="P5236_00560"
     CDS             4313..5671
                     /gene="tig"
                     /locus_tag="P5236_00560"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRU62891.1"
                     /translation="MNVIRKDLDQNNAVLTLSIKKDDYAENVEKRLRELRKKSNMPGF
                     RPGMVPLQLVRKMYGKSVLVEEINQLISQKLQDYIEENKIDILGEPLPREEDQQNIDF
                     NLQEEFEFNFDLGLAPQFEISFSKEDKIKYYHIEVTDEMIDNRIKAYASRFGKYQEEE
                     TVEEKDMLKGELVQLENGKVKENGIHITDAVLTPAYMKDDGQKSLFIGAKKGDTITFN
                     PATAYQNNETELSSLLKISKEEVNNIKEDFQFKIESITRFYPAEINQELFNKVYGENI
                     VSSEEEFREKIKQEIEKELAAESNYKFQLDVRTMLIDRYKNLNYPDEFLKRWLLLKNK
                     DLTPEKLEEEYPKIIEDVTWQLIREKLLKAFNINVEPDEVENYAKEEAKNQFAQYGFI
                     NLSEDILENYAKDMINKEDTLKNLIENLFDQKIFSSIKEVVTLETNNVTLEEFDKLFE
                     TK"
     gene            5789..6478
                     /gene="clpP"
                     /locus_tag="P5236_00565"
     CDS             5789..6478
                     /gene="clpP"
                     /locus_tag="P5236_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HRU62892.1"
                     /translation="MDKNNEFRKYATKKLGINGLLIDQYSDIVNTSYISPTILEERQL
                     NVTQMDVFSRLMMDRIIFLGTEVDDYAANVIEAQLLYLDSAEPGKDISIYLNTPGGSV
                     YAGLGIYDTMQFITSDVSTICTGMAASMGAVLLSAGAKGKRFALKHSRIMIHQPMGGA
                     QGQASDIEITAREIQKMKKELYNILAEHTGNPYEKIEKDADRDYWMTSYEALEYGMID
                     KVLINEKKTKK"
     gene            6488..7723
                     /gene="clpX"
                     /locus_tag="P5236_00570"
     CDS             6488..7723
                     /gene="clpX"
                     /locus_tag="P5236_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRU62893.1"
                     /translation="MPKTKKYCNFCGRSEDEVEFFILGMDGVQICNDCAMIAAEIAKE
                     NTQAKKQTLSFEKSQIPKPKEIKQYLDQYVIGQEEAKKYLSVAVYNHYKRLMQEKTDD
                     DVEIEKSNIIMVGPTGTGKTLLARTIAKKLHVPFTIVDATVFTEAGYVGEDVESILTR
                     LLQVADYDVEAAEKGIVFIDEIDKIARKSDNPSITRDVSGEGVQQGLLKLLEGTIVNV
                     PPQGGRKHPEQKMIPVNTQNILFICGGAFEGIERKIAARLNTRVVGYNSIEGTKQIDR
                     NNLIKYIMPQDLKSFGLIPEIVGRLPVLTYLNPLDRDALRQILTEPKNSIVKQYIKLF
                     KMDNIELTFDEEVLDYIVDKAIEFKLGARGLRSITETIMMDAMYEMPSKKAAKKLTIT
                     LDYAQNKLQQTNLDQLKIA"
     gene            7954..>8337
                     /locus_tag="P5236_00575"
     CDS             7954..>8337
                     /locus_tag="P5236_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRU62894.1"
                     /translation="MAKKTALAVHLKKYFGFDTFKGNQEEIIQNVLDGNDTFVLMPTG
                     GGKSLCYQLPSLIMDGTAIVISPLIALMKNQVDAMRNFSEEDGVAHFLNSSLTRQAID
                     QVKADVLSGKTKLLYVAPESLTKEEN"
BASE COUNT         2968 a         1206 c         1476 g         2687 t
ORIGIN      
        1 agtcacccat tcgtcgattc tttttgaacc tgacaatggg tgatgctttc ctgagtgacg
       61 atctggctca ggaaactttt atcaaagctt tcctttcttt gtctactttt aaggctttat
      121 ctgatttttc aacctggctt tttcgtattg cttataatgt gtattatgat gctgtccgaa
      181 caagaaaaga tttttatgat ctggaagaaa aagaaatcaa tcaacaatat caagtaaaca
      241 attcctattc aagcgacaaa gaagattttt acaaagcttt aatcaatttg aacaaaatgg
      301 aaaaaacagc cgttttgctt tattatatgg aagacaaaac acaacgtgaa attgcaacta
      361 ttatgaaatg tcctattggt agtgtaaaaa attatattcg aaaaggtaaa gaaaaaattg
      421 cgaatttttt aaaagaaaat ggatatggaa acgaatgaaa cagacaaaaa ttttcaggat
      481 ttctttaaac tacacaagca tgatatagct gataatggtt tcgccagcag aactttacgg
      541 catttgccgc agcgaaaaga caattcgtgg atcgtttggc tttttacaac cattggaatt
      601 gctacagctt tgtatattgg aattttctct gactggttga tgaactttat agaacatatg
      661 ttcaatttgc cattcattta tctgttaatt ggggttgcac ttttcccctt ggtaattgga
      721 ctttgcatta tgttctggga aaaagaacaa tattgaaaag cgattaatag gtggtaaaaa
      781 gcgtaagaaa ttaaatactg ttttaaacaa aaaagagaga aaattgactt tctctcttcc
      841 aaatttcttt ctttaataac gtttttcttt tatccttgct tttttacctg ttaacttacg
      901 taaataaaac agttttgcac gacgtacttt acctctttta tttacagtaa tgctttctat
      961 aaaaggcgaa tccatcggaa aaattctttc cacgccaaca tttcccgaca ttttgcggac
     1021 agtaaaacgt tttttccctt cctgtccttt aatgctgata actacaccac gaaattcctg
     1081 aattctttct ttgttccctt ctttaattcg ataggcaact gttatggtat caccactttt
     1141 aaattcaggg aaactttttt ctacctgaaa agcctgttct gcaaccttta ataagtccat
     1201 ttttattaat tttatttatt acagcacaat atttacatac ttttcgtttc tgccagagat
     1261 tgtgcttaaa ttggaacgca aaaatactat ttttttttat tgcatacaat aatttcatcc
     1321 ttttttgaat taaaaacttt tttaattgtt aactatcaat gatttaagtg taaaaaagct
     1381 tatctttgtt gttgatgaat tgaattgatg ctttttaaaa tttattatga gttgttgatt
     1441 tttaaaaggt tgataagatt tcaatcattt ttttattctt acatttttcc ctattccttt
     1501 gtttttatta tcctaaaagg aaatttcaag taattttttc ctttttttgt tgtccaataa
     1561 tagatgatta ttcaagtaat ttgaataata tagccaatta aaattcaatt tttttgatta
     1621 aatttgcaag cttaaaaaat gaggagttgg atacatgcaa aaaattagaa atgttgcaat
     1681 tattgctcat gttgatcacg gaaaaaccac gctggttgat aagatgttgt tggctggtca
     1741 tcttttcaga gacaatgaaa aggtcggcga attaatcatg gacaataatg aaatagaacg
     1801 tgaaagagga attacaattc tggcaaaaaa tgcttcgatc aattataatg gctataaaat
     1861 caatattatt gatacgccag gtcatgccga ctttggtggt gaagtagagc gggtactcaa
     1921 catggctgac ggagttttac tgttggtaga tgcttttgaa ggaccaatgc ctcaaacacg
     1981 atttgtttta caaaaagctt tgcaattggg acttattcct attgttgtga ttaacaaggt
     2041 ggataagcct aattgtcgac cggatgaagt tcaggaagaa gtttttgatt taatgtatca
     2101 tttgaatgcc acagaagaac aactgaattt ccatacaatt tttggttctg ccaaacaaaa
     2161 ttggatgtca gaagattgga gaaaaccaac aaacaatatt ttcccgctat tggatgctat
     2221 tataaaatat attcctgagc ctttctatct ggaaggaaca cctcaaatgc tgattacttc
     2281 tttggactat tctccttatg taggtagaat tgcagtggga cgtgttcatc gtggtacgct
     2341 gtacgaaggt atggatgtaa gtttagtgaa gcgagatggt tttgtggtga aaactaaaat
     2401 taaagaacta tacactttta tcggactgga acgaaaaaga atttcagaag taaaatcagg
     2461 agatatttgt gctttaatcg ggattgataa ttttgaaatt ggtgattcca ttgcagatat
     2521 taatcagcct gagcctttaa aacccattgc tgttgatgaa ccaaccatga gtatggtgtt
     2581 taccattaac aattctcctt tttatgggca ggaaggaaaa tacatcactt caaggcactt
     2641 acatgaacga ttgatgcgtg aattggataa agatcttgct ttgcgagtaa aaaaggaacc
     2701 tgataaagat atttggcatg tttatggcag aggtgtgctt catttatcta ttttaataga
     2761 aaccatgcgc cgcgaaggtt atgaattgca agtgggacaa ccgcaagtaa ttttcaagat
     2821 gataaatggc atccgttgtg aacctgttga acaactgata attaatctcc ctgaagaata
     2881 tagtggcaga attattgaaa ttatagcttc tcgtaaggga gaaatgttga aaatggaaac
     2941 gatgaacgat cgtattcaat tagaatttat aattccttca cgaggcatta ttggacttcg
     3001 caatcaagtg ctaactgcat caggcggtga agctattatg agtcatcgtt ttcttgaata
     3061 tcaaccttat aaaggaccta tcgaaaaaag aacaaatggt tcattaattg caatggaaac
     3121 aggcacagct tttgcatacg cattggacaa attacaagat cgaggtagat tttttatttc
     3181 accccaagaa gaagtttatg cgggacaggt ggtaggagaa agctcaaaag agggtgatat
     3241 tgtagtaaat gtcactaaat ctaaaaaatt aaccaatatg agagcttctg gctcagatga
     3301 aaaaatcaaa cttactcctc ctgtcatatt tagtctcgaa gaagcgttgg aatacataaa
     3361 agaggatgaa tatgtggaaa ttactccttc taatattcga ttacgaaaaa tctatcttga
     3421 tgagaatgaa cgaaaacgaa tgtcaaaaaa tgtttctgct actgcataat taaattcttt
     3481 tatttctcat tgatatttaa aacaaggaag tacaaaaatt tatttttcat gaaaaaagaa
     3541 ttttgggttg tattatttct tttactgata tttagttcaa aaattttagc tcaatttggc
     3601 gtgaaaatcg ggatcaatga agcaaatgaa gttattactt caagtaattt atctactccc
     3661 atcgaaaagc ctactttgca gaacttaact gcttatcaca taggagtaat ttatcaatat
     3721 acttcaaaaa ttggcttagg ctttgatatt ggcacatctc tctctcaaaa aggttgtttc
     3781 cttcaatcag acagcatttg tgatatctat agagaattaa actatattga aatacctttc
     3841 aatattcgtt atcgtttatc atggggaatc atcggaattt atggctttgg aggtttatat
     3901 ggagcctatc ttttaaaggg acaaaaaatt actgaaacaa atacttcttc tattccagaa
     3961 aaattttctt ataccaattt caatgatcga accgattatg gctatacctt tggtggcggg
     4021 attgaaattt ttaaaaaaat acaattggaa gcgaactgga ataaaggctt gaaagaaaat
     4081 atttattata caaattcaga cacacaaatt tttactacaa aaaataatgt cttctctttt
     4141 aaccttacat ttatattcta attatgaata atttattaat tatttgatta tcaaataatt
     4201 aaatttattc atagagtttt ttaaacataa aatgttttct atcagttctt ttttcgtacc
     4261 tttgtcttca ttaaaaaact aaatatcaga aaattaatta aaaaaataag ttatgaatgt
     4321 cattagaaaa gaccttgatc aaaacaacgc tgtacttact ttaagcatca aaaaagacga
     4381 ttatgcagaa aatgtagaaa aaagattgcg ggagttacgt aaaaaatcaa atatgccagg
     4441 atttcgtcca ggaatggtac ctttgcagtt ggtaagaaaa atgtatggga aatcagtcct
     4501 tgtagaagaa attaaccaac tcatatctca aaagctgcaa gattatatcg aagaaaacaa
     4561 aattgatatt ctgggcgaac ctcttccacg agaagaagat cagcaaaata tagattttaa
     4621 cttacaggaa gaatttgaat ttaattttga tttaggattg gctcctcaat ttgaaatatc
     4681 attttccaaa gaagataaga ttaaatatta ccacatcgag gttaccgatg aaatgattga
     4741 caatagaatc aaagcgtatg cttctcgttt tggaaaatat caggaagaag aaaccgttga
     4801 agaaaaagac atgttgaaag gagaattagt gcaattggaa aatggtaaag tcaaagaaaa
     4861 tggaattcat attactgatg cagtacttac accagcttat atgaaggatg acggtcaaaa
     4921 atcattgttt atcggtgcaa aaaaagggga cacgattact tttaatccag ctactgctta
     4981 tcaaaacaac gaaacagaat tatcttcctt attaaaaatt tcaaaagaag aagtaaacaa
     5041 tattaaagaa gattttcaat ttaaaattga gagtattact cgtttttatc ctgctgaaat
     5101 caatcaagaa ttattcaata aggtttatgg tgaaaatata gtgtcaagcg aagaggagtt
     5161 tcgtgaaaaa ataaaacaag aaattgagaa agaacttgct gctgaaagta attataaatt
     5221 tcaattggat gttcgtacga tgttgataga tcgatataaa aacttgaact atcctgatga
     5281 atttttaaaa cgctggttat tgctcaaaaa taaagaccta acgccagaaa aattggaaga
     5341 agagtatcca aaaatcattg aagatgttac ctggcaattg attagagaaa aattgctgaa
     5401 agcttttaat ataaatgtgg aacccgatga agtagaaaat tatgccaaag aagaagcaaa
     5461 aaaccagttt gcgcaatacg gatttattaa tcttagcgaa gacatccttg aaaattatgc
     5521 aaaggatatg ataaataaag aggatacttt aaaaaactta attgagaatt tgtttgatca
     5581 aaagattttt tcttccataa aggaagttgt aactttagag acgaataatg ttaccttaga
     5641 agaatttgat aaattgtttg aaaccaaata attttaaaag acaatttgaa tttagaattg
     5701 aaactgaaac ttttttaaaa taattttgtt tataaaaagt tctctaccaa tgagaacgat
     5761 aaatttttta taaaaacaaa taactattat ggataaaaat aatgaatttc gcaaatatgc
     5821 aaccaaaaag ttgggaataa atggcttgtt aattgaccaa tacagcgata ttgtaaatac
     5881 aagttatatt tctcctacca ttcttgaaga acgtcaactg aatgtcactc aaatggatgt
     5941 gttttcaaga ttaatgatgg atcgaattat ttttctgggt accgaagtag atgattatgc
     6001 tgccaacgta attgaagctc aattacttta tcttgattca gcagaaccag ggaaggatat
     6061 ttctatttat ctgaatacac caggaggttc agtttatgct ggtctgggta tttatgatac
     6121 tatgcagttc attacttctg atgtttctac tatctgtact ggcatggcag cttcgatggg
     6181 agcggtacta ttatctgctg gtgcaaaagg gaaacgtttt gctcttaaac attcacgtat
     6241 catgattcat cagccgatgg gtggagctca agggcaagcc tcagacattg aaatcacggc
     6301 tcgtgaaatc cagaaaatga aaaaagaact ctacaatatt ctggcagaac atacgggcaa
     6361 tccttacgaa aaaattgaaa aagatgctga tcgagattat tggatgactt cttacgaagc
     6421 cctggaatat ggaatgattg ataaagttct catcaacgaa aagaaaacaa aaaaataatg
     6481 cttaaaaatg cctaaaacca aaaaatattg taatttttgt ggtcgttcag aggatgaagt
     6541 agaatttttt attttaggaa tggatggcgt tcagatttgc aacgattgtg ctatgattgc
     6601 cgcagaaatt gcgaaagaaa acacccaagc caaaaaacaa actctttctt ttgaaaagtc
     6661 gcaaataccc aagccaaaag aaataaaaca atatctggat caatatgtta tcgggcaaga
     6721 ggaggcaaaa aaatatttat cagtagccgt ttataatcat tataaacgtt taatgcagga
     6781 aaaaaccgat gatgatgtag aaatagaaaa atccaacatt atcatggtag gacctacagg
     6841 tactggtaaa accttgctgg caagaacaat agcaaaaaaa ctacatgtac cttttactat
     6901 tgttgatgca acggtattta ctgaagcagg ttatgtgggg gaggatgtag aaagtattct
     6961 tacacgactt cttcaggtag cagattatga tgtagaagct gctgaaaaag gtattgtttt
     7021 cattgatgaa atcgataaaa tagctcgaaa aagtgataat ccaagcatta cccgcgatgt
     7081 gagtggtgaa ggagtgcaac agggtttgtt aaagttgttg gaaggaacta ttgtcaatgt
     7141 accaccacaa ggaggtagaa aacatccaga acaaaaaatg attcctgtaa atacacagaa
     7201 tattttgttt atttgtggcg gagcctttga aggcatcgaa agaaaaattg cagctcgtct
     7261 caataccaga gtggtcggat ataattcaat agaaggcact aagcaaattg atcggaataa
     7321 tcttataaaa tacattatgc ctcaagatct caaatctttt ggactaattc cggaaattgt
     7381 aggccgtctt cctgttttga cttatcttaa tcctttagat agagatgctt tgcgtcaaat
     7441 tcttactgaa cctaaaaatt ctattgtcaa gcagtatatt aaactgttta aaatggacaa
     7501 tatagaatta acttttgatg aagaagtttt agattacatt gtggataaag ccattgaatt
     7561 caagttgggt gccagaggat tgcgttccat tacagaaaca attatgatgg atgccatgta
     7621 tgaaatgcca tcaaaaaaag cagcaaaaaa gctaactatt acgttagact acgctcagaa
     7681 taaattgcaa caaaccaact tggatcaatt aaaaatagcc tgaaataata tttctaaaat
     7741 tttcattaaa aagcaggata ttaattattt ttattaatgt cctctttttt ttaaaaaatg
     7801 cttgtatatt tgaaaagtgt ttataatttt gtttgcctgt aaatgtgaaa aaataaatca
     7861 agaaaggaaa taaaagattt ttcattaaag tagaaaaatt catactaatt tttaaatact
     7921 aattttactt attttgtgat cgaggattgt tgtatggcta agaaaactgc tttagcagtg
     7981 catttaaaaa aatattttgg ttttgatact ttcaaaggta atcaggaaga aattattcaa
     8041 aatgtattgg atggcaatga tacttttgtt ttaatgccaa caggaggagg taaatcttta
     8101 tgctatcagt tgccttcttt aataatggat ggcacggcga tagttatttc acccttaatt
     8161 gctttaatga aaaatcaggt agatgccatg cgtaatttca gtgaagaaga tggtgtggca
     8221 catttcctta attcatcatt aacacggcaa gcaattgatc aagttaaagc tgatgtttta
     8281 tcaggtaaga cgaaattgct ctatgtggct cctgaatcac ttacgaaaga agaaaat
//
LOCUS       DAOTKS010000013         8292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_8638_length_8292_cov_4.326454, whole genome shotgun sequence.
ACCESSION   DAOTKS010000013 DAOTKS010000000
VERSION     DAOTKS010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8292
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8638_length_8292_cov_4.326454"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(265..771)
                     /gene="purE"
                     /locus_tag="P5236_00580"
     CDS             complement(265..771)
                     /gene="purE"
                     /locus_tag="P5236_00580"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRU62895.1"
                     /translation="MNPRVSIIMGSTSDLKVMEKAAKLLDEFEIFFEMNALSAHRTPE
                     EVEDFAVHAEGRGIEVIIAGAGMAAHLPGIIASMTTLPVIGVPIEASMNGIDALLSIV
                     QMPPGIPVATVGINASLNASILAIEMLALKDENINVRLKSYKKNLKQKIVEANEQLKE
                     VKYRFKTN"
     gene            complement(799..1176)
                     /gene="gcvH"
                     /locus_tag="P5236_00585"
     CDS             complement(799..1176)
                     /gene="gcvH"
                     /locus_tag="P5236_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRU62896.1"
                     /translation="MKVPENLKYTKDHEWIRLEDDIAYVGITDFAQKELGDIVFVEVE
                     TVGEQLSAGDVFGTVEAVKTVSEIFLPVDGEILAFNKELENAPELVNEDPYGKGWIVK
                     IRVTNPSQYDTLLTATDYKNLIS"
     gene            complement(1246..2337)
                     /locus_tag="P5236_00590"
     CDS             complement(1246..2337)
                     /locus_tag="P5236_00590"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRU62897.1"
                     /translation="MIKSVVKWLLLAINIGVALCLFFALLAANIHPQYFILPSYFALL
                     FPVIFILNGAFFIFWLIFRSWYFLLSFCFLLLSIPQLKNNFSFRFKQPQKIEAEQPIT
                     LLSYNTRMMDLLKKYEPKEENKVLQYVLTSDADIVCLQEFMVSPNEKYLTEKEVLSLF
                     NKYPYHHIYYRVKQKSSWLGIATFSKFPIVNQQTIAYVSSANASIFTDINIHGKIIRI
                     INNHLESNQLTEKDKSLPYQLKNNLDTKLIHDITLVFSKKLGAAYKIRASQADSVAVV
                     VKNSPYPLLVCGDFNDVPGSYTYCKIKDHLNDSFLERGKGFGWTFIERYYRFRIDYIF
                     HDSFFQLVDFKIDKVRYSDHYPLLAKFSI"
     gene            complement(2387..3277)
                     /locus_tag="P5236_00595"
     CDS             complement(2387..3277)
                     /locus_tag="P5236_00595"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018669332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRU62898.1"
                     /translation="MNVFKNIKSSFRRSTSLTKLLSINIGIFILYQLVLIVLKLFNLQ
                     GLFLAEYLALPSSLMQVATHFWTLVTYMFFHAGFLHIFFNMLCLYWFGKIFLMYFSEK
                     QLVGLYLMGGWMAAFFYIFSFNIFPYFKSVVNSSILLGASGSIMAIIVAAAVASPNLE
                     IRVLLLGNIKLKYIAIVAVLTSFFGITSNNAGGEIAHLGGALCGYLFVVSLHKGKDMT
                     KWLNNILDNIVNLFSWKPAKKRKKIYYTERKMTDEEFNIEKTRRMKEIDRILDKIKES
                     GYDSLTKEEKKILFDQSNKF"
     gene            complement(3332..4108)
                     /locus_tag="P5236_00600"
     CDS             complement(3332..4108)
                     /locus_tag="P5236_00600"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRU62899.1"
                     /translation="MSNKSSFLGSIPIVVKNLIFINIILWLLSIVSPSLFARWGLKVD
                     LMDILGMHYWASEKFNVAQLITYMFMHGGFEHIFFNMFALFMFGTVLEQTWGSKRFLI
                     YYLVTGIGAGLIQQLVWAIQYQPVVTALNEAIASGSIQTLLMHQDVLNRFFRINNLSG
                     FDTASLIELKRLFLNLPVTIGASGSIFGLLLAFGWLFPDARLMIIFFPIPIRARIFVL
                     MYAVVELFLGVASFSGDNVAHFAHLGGMLFGIILILLWKK"
     gene            4452..4724
                     /locus_tag="P5236_00605"
     CDS             4452..4724
                     /locus_tag="P5236_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRU62900.1"
                     /translation="MNKAELVNAVAAKSGLSKVDAKKALDAVIKSISEALVSGDKVSL
                     VGFGTFSVVERSARAGINPATKKPIKIPAKKVAKFKAGSELANAIK"
     gene            complement(4840..5385)
                     /locus_tag="P5236_00610"
     CDS             complement(4840..5385)
                     /locus_tag="P5236_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRU62901.1"
                     /translation="MKAIIFLAEGFEEIEAIAPIDILRRAGIEVVTVSVSDKNDVCGS
                     HGILIKADALFKEIDFSTADLLCLPGGMPGTKNLDNHQELKDLIVCHANGGKNLGAIC
                     AAPSILGKLGLLSGKEAICFPGFEDQLVGAKLSNKKVVRDGNIVTAKAAGVAVEFALK
                     LVEMLKGKSASDQVAASIFAN"
     gene            complement(5403..6128)
                     /locus_tag="P5236_00615"
     CDS             complement(5403..6128)
                     /locus_tag="P5236_00615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRU62902.1"
                     /translation="MRKSQIYGIIGSAISCGIILLLLFVIKLSAPSNMPQEEGIVVSF
                     GESEEDMGRSKEQGNHLTFKGAASQQISSDKNEKLLTQSESSVALSEQKNDKRQSENI
                     SEQEELRKKRENEAIEKANVIATIFGNGGSSEGSSGNDNILQGNPAGKGVSNGHGWSL
                     SGRDLVGGLVTPRYEKNVEGKITVNIRVDENGKVVGASIGSPTTISDADIRKAAIEAA
                     LNTRFTTGKGMVSGTITYNFKLK"
     gene            complement(6130..6546)
                     /locus_tag="P5236_00620"
     CDS             complement(6130..6546)
                     /locus_tag="P5236_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRU62903.1"
                     /translation="MALKRKNKVEAGFVMVSMTDIIFLLLLFFVIASTLSSPNDIKID
                     LPQSKAKTSTPVITKVFIDGKSNYFVAHGNEKPIPITADLLESYIVKLAAQDSMTHIA
                     LYADQNIPYRNVVRVLDIANQHKIKIVIATKPFEEK"
     gene            complement(6629..7321)
                     /locus_tag="P5236_00625"
     CDS             complement(6629..7321)
                     /locus_tag="P5236_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRU62904.1"
                     /translation="MNLLVILQAASDVATTVAEQTLPKMNLWTMTKYGGPIMIILAAM
                     LAIAIYLFIERLITINEAAKEDSFFMERIKDYIHDGKIETALKLCRETNFPTARMIEK
                     GITRLGRPMNDILVAIENVGNMEIAKLEKGLVIMASIAGGAPMLGFLGTVTGMVITLY
                     NMSMKAGGTILLGDLSVGMYQAMVTTIGGLIVGILAYFAYNYLVSKIDSVVTLLESRT
                     MEFLDLLNEPVK"
     gene            complement(7395..8108)
                     /locus_tag="P5236_00630"
     CDS             complement(7395..8108)
                     /locus_tag="P5236_00630"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HRU62905.1"
                     /translation="MTKLSVNVNKVATLRNARGGNIPNVCQVAIDCERFGADGITVHP
                     RPDERHIRYADVWELKSVLKTEFNIEGYPSEKLLNLVKSVKPHQVTLVPDAPEAITSN
                     AGWDTVKNFDFLKDIVAELQEEGIRVSIFVDTNLKNIEYASLTGTDRVELYTEPYARE
                     YSTNREKAIAPFIEAAQLAKKLGLGLNAGHDLNLQNLRYLHENIPWLDEVSIGHALIS
                     DALYFGLQETIKQYKNQLL"
BASE COUNT         2904 a         1509 c         1131 g         2748 t
ORIGIN      
        1 gtcaaataaa gaagtaatcc aataattatc aataagatac ctataattat aaaccatttc
       61 cacattttca tttttttaaa tttttacttc tttaaattca aaaaaatctt tctgattcag
      121 caaatataga gataaaattc atccaacaat tacttcgttc tgtcggatta taaatctttt
      181 ataagaaaat ttagttgata tccaaaagtt tgcgaattag ataattcttc caacaaaaaa
      241 tagtagtaaa aagtctgaaa aatattaatt ggttttgaaa cgatatttca cttctttaag
      301 ctgctcatta gcctcaacaa ttttttgctt caaatttttc ttgtatgatt ttaaacgaac
      361 atttatattc tcatctttaa gtgcaagcat ttcaattgct aatatagagg cattcaacga
      421 agcattgata cctactgttg ctacggggat tcccggtggc atttgcacta ttgaaagcaa
      481 agcgtctatc ccattcatag aagcctcaat aggaacgcca atgacgggca aagtagtcat
      541 ggaagcaatt atacctggca agtgagctgc cattcctgca ccagcaatga taacttctat
      601 tcctctcccc tctgcatgaa cggcaaaatc ttctacttct tctggagtac gatgggctga
      661 taaggcattc atttcaaaaa agatttcaaa ttcatccagt aatttagcag ctttttccat
      721 cactttcaga tcggaggtac tacccataat gatacttact cgtggattca tttttaatga
      781 tttttaaatt attaaaattc aactaattag attcttgtag tcagtagcgg ttaataacgt
      841 atcatattga gaaggattag taacacgaat ttttactatc catcccttac cgtaaggatc
      901 ttcgtttacc aattcaggtg cattttccaa ctctttatta aaagccaaaa tttcaccatc
      961 tactggcaaa aatatttctg aaacggtttt tactgcttct actgttccaa aaacatcgcc
     1021 tgctgacaat tgctcaccta ctgtttcaac ttctacaaat actatatcgc ctaattcttt
     1081 ttgagcaaaa tctgtaatac caacataagc gatatcgtct tcaagcctta tccattcatg
     1141 gtctttggta tacttcaaat tttcaggtac tttcatttct aataatatat taaaagttaa
     1201 acaaatgtat agtgttcagt aaattaatta gctcttattt ttgagttaaa tagaaaattt
     1261 tgctagtaac ggataatgat ctgaatatct caccttatca attttaaaat caactaattg
     1321 aaaaaaagaa tcatgaaaaa tataatctat tctgaaccga taataacgtt ctataaaagt
     1381 ccatccaaat cctttccctc tttccaaaaa actgtcattc aaatgatctt taattttaca
     1441 ataggtgtat gagcccggaa catcattgaa atcgccacac accagaagag gatatggtga
     1501 atttttaaca acaactgcca cagaatcagc ttgggaggct cttattttat aagctgcacc
     1561 taattttttt gaaaacacaa gcgtaatgtc atgaattaat ttcgtatcca gattattttt
     1621 caattgataa ggaagcgatt tatctttttc agttaattga ttggattcaa gatgattatt
     1681 aatgattctg atgattttac catgaatatt tatgtcagtg aaaatagaag cgttggcaga
     1741 cgagacataa gcaatagttt gttgattaac aataggaaat tttgaaaaag tagcaattcc
     1801 taaccagctt gacttttgct ttactctata ataaatatga tgataaggat atttattgaa
     1861 taaactcaat acctcttttt ctgtaaggta tttttcattg ggactaacca taaattcttg
     1921 aagacaaaca atatctgcat cacttgttag aacatattga agtactttat tttcttcttt
     1981 cggttcatat ttttttagca aatccatcat tcttgtattg taagaaagta aagtgatggg
     2041 ttgctctgct tcaatttttt gtggctgttt aaaacgaaag gaaaaattat tttttaattg
     2101 gggaatagat aataacaaaa aacaaaaaga aagtaagaaa taccagctcc gaaaaatgag
     2161 ccaaaaaata aaaaaggctc cgttaagaat aaaaatcaca ggaaatagca gagcaaaata
     2221 agatggcagt ataaaatatt gaggatgaat attagcagct aataacgcaa aaaacaaaca
     2281 aagagcaact ccaatattaa tagccaacaa caaccatttg acaacacttt taatcatata
     2341 tacttatcag tttaatcatt caaaattata caaaaactga taaaaattaa aatttattac
     2401 tttgatcgaa taatattttc ttttcttctt ttgttaagct gtcgtagcct gattctttta
     2461 ttttatcaag gattctgtct atttctttca ttcggcgggt tttttcaatg ttaaattcct
     2521 catccgtcat ttttctttct gtataataaa tttttttcct ttttttagca ggtttccatg
     2581 aaaataaatt aacaatatta tcaagaatat tattaagcca ttttgtcata tcctttcctt
     2641 tatgcaaaga tacaacaaaa agataaccac ataaggcacc tcccaaatga gcaatttcac
     2701 cgcctgcatt attggaagta ataccaaaaa aactggttag aacagccaca atggcaatat
     2761 attttaattt gatatttcct aacagcaaaa ctctgatttc caaattcggc gaagccactg
     2821 ctgcagctac aataatagcc atgatagacc ccgaagctcc taaaagtata ctgctattta
     2881 caaccgattt aaaataagga aagatattaa aggaaaaaat ataaaaaaaa gctgccatcc
     2941 aaccacccat aagatataaa ccaactaatt gtttttcaga aaaatacatt agaaatattt
     3001 tcccaaacca ataaaggcac aacatattaa aaaaaatgtg gagaaagcct gcatgaaaaa
     3061 acatgtaagt tactaacgtc cagaaatggg ttgctacttg cattaggctg gaaggcaatg
     3121 ctagatactc tgccagaaac aatccttgca aattaaaaag tttcagaaca atcagtacca
     3181 attgataaag gataaaaatc cctatgttga tagataataa tttagtgagc gaagtacttc
     3241 gccgaaatga agattttatg tttttaaaaa cattcataaa tttgaaaaga aaatcagtac
     3301 tcaaaacaac tcaattaaaa tgttttttgt ctcacttttt ccacaacaaa atcaggataa
     3361 ttccaaaaag cattccaccc aaatgagcaa aatgagccac attatcccct gaaaagcttg
     3421 ccactcctaa aaataattct acgactgcat acattaaaac aaatattcgt gctctgatgg
     3481 ggatggggaa aaatataatc atcaatcttg catccggaaa aagccaacca aaagccagca
     3541 atagaccaaa aattgagccc gaagcaccaa ttgtcaccgg taaatttaaa aatagacgtt
     3601 ttagttcaat caatgaagcg gtatcgaaac ctgataaatt attgattcta aaaaaacgat
     3661 ttaagacatc ttgatgcatc agtaaagttt gtatagaacc tgatgcaata gcttcattga
     3721 gagctgttac aacgggttga tattgaatag cccaaaccag ttgctgaatt aatcctgctc
     3781 caatacctgt tactaaataa taaattagaa aacgttttga tccccatgtt tgctccaata
     3841 cagttccaaa cataaacaag gcaaacatat taaaaaatat atgttcaaaa ccgccatgca
     3901 taaacatata agtgataagt tgagctacat taaatttttc agaagcccaa taatgcatgc
     3961 ctaaaatatc catcaaatca acttttagtc cccaacgagc aaataaactg ggagatacta
     4021 ttgatagcaa ccataagatt atattgataa aaattagatt ttttactacg atgggaattg
     4081 aacctaaaaa agaagactta ttgctcattt atttattttt ttactatatt tacagccact
     4141 ttaaaattat ttcaaaaact aaaaaatatt cacaaaatta tcaagttatt agtagcaagg
     4201 attttttatg gtgttaacaa aaaaaccttt taataggaat aataactttc taaaaaacga
     4261 gttaaataat aaatttttag cacaaaagta atgaaaaata aaacaaaaat atgttttcta
     4321 acatattttg taagaaaata taaaaaaatg actaattttt tatataaaat atttgcatat
     4381 tacttgtaat aatgtacatt tgcagaatat aaagaatttt ttacttgttt aatttttaaa
     4441 aaatttatct tatgaacaaa gcagaattag tcaatgctgt tgcagcaaaa tcagggctta
     4501 gcaaagttga tgctaaaaaa gctcttgacg ccgtaattaa aagtatttct gaagcactcg
     4561 ttagtggtga caaagtaagt ttagttggtt ttggtacttt ttcagttgtt gaacgttcag
     4621 cacgcgctgg tatcaatcct gctaccaaaa agccgattaa aattcctgca aaaaaagttg
     4681 caaaatttaa agcaggttct gaacttgcca atgctattaa atgatttttt aaatcaggca
     4741 acattacata agagaatgaa aaatattagt atttttcatt ctcttttaaa agcaaaaagt
     4801 tttatcaaaa ttagtttttt tatcattctt tatattcgct cagttagcaa aaatcgaggc
     4861 agcaacttga tctgaagctg attttccttt taacatttcc accaatttga gagcaaattc
     4921 cactgctact cctgcagctt tggctgtgac gatatttcca tcccttacaa ctttcttatt
     4981 cgataacttt gcacctacca attgatcttc aaaaccagga aaacaaatag cctcttttcc
     5041 actcaataag cctaattttc ctaaaataga aggagctgca caaatagcac ctaaattttt
     5101 cccaccattt gcatgacaaa caattaaatc tttcaactcc tgatgattat caagattctt
     5161 tgttcccggc atgcctccgg gcaaacataa taaatctgct gttgaaaaat caatttcttt
     5221 aaataaggcg tcagctttta ttagtatgcc atgagaccca caaacgtcat ttttatctga
     5281 gacagaaacc gtaacaacct caatcccagc tcttctcaaa atatcgatcg gagcaatggc
     5341 ttcaatttct tcaaagccct ctgctaaaaa tatgattgct ttcataattt ttcactgtta
     5401 ttttatttta atttaaaatt ataagtaata gtcccgctta ccattccttt accagttgta
     5461 aaacgggtat ttaaagctgc ttcaatagct gcttttctaa tatcagcatc agaaattgta
     5521 gtaggagaac caatggaagc acctactact ttcccatttt catctactct aatatttacc
     5581 gtaattttac cctctacgtt tttttcatag cggggagtta caagccctcc aaccaaatcc
     5641 cttccactca gtgaccagcc atgtccattt gaaactccct tacccgctgg attgccctgt
     5701 aaaatattat cgtttcctga actaccttcc gaagagcctc cgttaccaaa aatagttgca
     5761 atgacattgg ctttctcaat tgcttcattt tctctttttt tcctcaattc ttcctgctca
     5821 gaaatatttt cgctttgtct tttatcattt ttttgttctg ataatgctac cgaagattct
     5881 gattgagtca ataatttttc atttttatct gatgaaattt gttgggaagc agccccttta
     5941 aatgtcaaat ggttgccttg ttctttactt cttcccatat cttcttcact ttctccaaaa
     6001 ctgactacaa ttccttcttc ttgtggcatg ttagatggag cagataattt tataacaaaa
     6061 agcaataaaa gtattatccc acagctaatt gctgatccaa tgatgccata tatttgagac
     6121 ttcctcacgt tatttttcct cgaaaggttt tgtcgcaata actattttga ttttatgttg
     6181 gttagcaata tctagcactc ttaccacatt tcgataagga atattttgat ccgcatataa
     6241 agcaatatgg gtcatggaat cttgagctgc caacttcaca atataagatt ccaataaatc
     6301 agcagtaatt ggtataggtt tctcattccc atgagcaaca aaataatttg atttcccatc
     6361 aataaatact tttgtaatta cgggtgttga agttttagct ttactttgag gtaaatctat
     6421 ttttatatcg ttgggtgaag ataaagtaga agcaatgaca aaaaaaagca gcaacagaaa
     6481 aataatatct gtcatcgata ccattacaaa acctgcttca actttatttt ttctttttaa
     6541 agccatgaaa aaatgaatta aattttttta taaacgataa gaaaaactca aaagcgaatc
     6601 ttttaatttt cgtataaaat accgaagttt attttacagg ttcattcaga agatctaaaa
     6661 attccatagt cctcgattcc agaagagtta ccacactatc aattttagaa accagataat
     6721 tataggcaaa ataagccaaa attcccacaa taagtccacc aatagtagta accatagcct
     6781 gatacattcc taccgacaaa tcgcccaaga gaatagtacc accagctttc atagacatgt
     6841 tatacaaagt tattaccatt cctgtaactg ttcctaaaaa gcccaacatt ggagcaccac
     6901 cagcaataga agccatgata acaagacctt tttctaattt tgcaatctcc atatttccta
     6961 cattttcaat agcaaccaaa atatcattca tgggtcgtcc taaccgagtg attccttttt
     7021 caatcatccg agcagttgga aaattcgttt cacggcaaag cttcaaagca gtttcaattt
     7081 taccatcatg aatataatcc ttaatccttt ccataaagaa agaatcttcc ttagcagctt
     7141 cattaatggt tattaacctt tcaatgaata aataaattgc aatggcgagc attgctgcca
     7201 gaataatcat gattggacct ccatatttgg tcattgtcca taaattcatt ttaggtagag
     7261 tttgttcagc aacagttgtt gccacatctg atgcggcttg caaaataact aatagattca
     7321 tttgaaatca attttaatcg ttttttctga tttttttaca aaatatgtgc cttttttatt
     7381 cctttatgta tcatttacaa taactgattt ttatattgct ttattgtttc ttgcagtcca
     7441 aaatataaag catctgaaat taaagcatga ccaatagaaa cttcgtccag ccaggggata
     7501 ttttcatgta aataacgaag attttgaagg ttcaaatcat gcccggcatt taaccccaag
     7561 cctaatttct ttgctaattg agcagcttca ataaaaggag caatagcttt ttctcgattt
     7621 gttgaatatt ctcttgcata aggttccgtg tataattcca ctcgatcagt acctgtaagt
     7681 gaagcatatt ctatattttt taagttggta tcaacaaaaa tagaaacacg gataccttct
     7741 tcttgtaatt cagccacaat atcttttaaa aaatcaaaat ttttaacagt atcccaccct
     7801 gcattcgaag taatagcctc aggcgcatcg gggaccaatg taacttgatg aggtttaact
     7861 gattttacta aattcaaaag cttttccgaa ggataacctt ctatattaaa ttcggttttt
     7921 aaaacggatt ttaactccca aacatctgca taacgaatat gtcgttcgtc aggacgtgga
     7981 tggaccgtaa taccatctgc accaaaccgt tcacaatcaa tagccacctg acacacattc
     8041 ggtatgtttc ctcctcttgc atttcgtaag gttgctactt tattaacatt tacacttaat
     8101 tttgtcatat caacttaatt ataaaattat tgtgagtaaa tttttatgcc cctaatttta
     8161 acaatatacc aaataataca ttttagctct ctaacttcgg taaattaaaa gctatttagt
     8221 ttattggtaa aattaaattc aacacaaaaa tagtttaaaa aattcaagat aatatggaaa
     8281 tatttctact tt
//
LOCUS       DAOTKS010000014         8258 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_8681_length_8258_cov_4.849689, whole genome shotgun sequence.
ACCESSION   DAOTKS010000014 DAOTKS010000000
VERSION     DAOTKS010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8258
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8681_length_8258_cov_4.849689"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="P5236_00635"
     CDS             <1..228
                     /locus_tag="P5236_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRU62906.1"
                     /translation="CFEKKGVLLNEEDEESVIAELNSSLFEQYVAEKVIQGGMIPKLQ
                     NSFDALKAGVKEVWITRADQLNRKKGTKITL"
     gene            464..1522
                     /locus_tag="P5236_00640"
     CDS             464..1522
                     /locus_tag="P5236_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HRU62907.1"
                     /translation="MVDTLTNEAVELLQKMISIPSFSREEKELADFLEFYLQAKGYDV
                     FRNKNNIWLISPGFSPLRPTLLLNSHIDTVRPANDWTLNPFEPKIENGKLYGLGSNDA
                     GASVVSLLSAFLYLIQQNQSYNLIFAATAEEEISGENGIECLLEEFPPIDFAIVGEPT
                     GMKMAVAERGLMVLDCIARGKSAHAGREEGENAIYKAIEDIEWFRTYQFPKQSDFLGA
                     VKMTVTQIAGGTQHNVIPDTCTFVVDVRSNELYTNEEILEIIREHVHCEVEPRSIRLC
                     SSNVQLDHPIITRARELHLDLFGSPTLSDQALMPFPSVKIGPGDSARSHTANEFIELN
                     EIEKGIQIYIQLLNGLKW"
     gene            complement(1534..2490)
                     /locus_tag="P5236_00645"
     CDS             complement(1534..2490)
                     /locus_tag="P5236_00645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HRU62908.1"
                     /translation="MDFVERSKEIFALEITELQKLANRLGPEINEAVEMIYNCKGKLV
                     IMGIGKTGIIGHKIASTLASTGTPTIFINAAEAVHGDLGMVSKNDIVLLISNSGSSSE
                     ILNVIFPLKNIGCTIIAMTGNPKSPLAKEASLILNVGVEREACPLGLAPTTSTTATLL
                     MGDALTICLMERRNFKAENFALYHPGGALGRQLLAQVKDEMVTDIPKVYTQTPFKDVI
                     YEVSNKRLGMTMVYNGNEEAVGIITDGDIRRAIQKFDNIKHLTAADFMTHGFKRITQE
                     ALLSEALEIMDENNITNLAVMETPDSDKVIGVITIHNIIDFK"
     gene            complement(2496..3062)
                     /locus_tag="P5236_00650"
     CDS             complement(2496..3062)
                     /locus_tag="P5236_00650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HRU62909.1"
                     /translation="MQIDNLIKNSSFKINNISAETDLTFSQNFCHKIKLFLCDVDGVM
                     TDGGMYYSELGDELKKFNVSDGMGLKLIQKQGIKVGIITSENTKIVERRFEKLKLDYL
                     YQGNREGGKLASAKEICEKEGITLDEVAYIGDDVNCVELLSNVGLAACPADAQQAVKN
                     IPGILQMKKKGGEGCIREFVELIMKYYC"
     gene            complement(3039..3866)
                     /gene="kdsA"
                     /locus_tag="P5236_00655"
                     /pseudo
     CDS             complement(3039..3866)
                     /gene="kdsA"
                     /locus_tag="P5236_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012364209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
     gene            complement(3915..5240)
                     /locus_tag="P5236_00660"
     CDS             complement(3915..5240)
                     /locus_tag="P5236_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HRU62910.1"
                     /translation="MNQFQKKPLKKITSTSKFEKNEATLLKDMQRYFEISRSAELEEY
                     KNLFHTISSSEFQEKKKLLKNRKYQDTEEYQIVTQYRKLQKSPAIRLYYETLQSPLLQ
                     EYLQFKKTSEYEDLGDKKKVNASKTLQKFKEFEHSKAYKNYIRFHDSYIIKQYEELKE
                     KVNLPEFKESNEFWANANRWHATPEYALQQRYHQLRKNPDIVYYENEKPERFEKYRNL
                     KLTFEENFDWHTLDRSRWSYGFHYQHPVLIQDHSFANEKQAYNKGKNVVVENGILKIY
                     TKKEKVTARAWDPVKGFILKDFEYTSDIIQTADFFRQQRGVFRAKLRCSGKIHHAFWL
                     GTDRKLPQITIFHFDGKNINVGNAHESGFDGNKIKGIHPSQFYIYTLVWTDNELIWFI
                     NDREVYRTSSNLPQEKMYLAFNSFIPEKEKGDEGILEVDWVKVYQFKKE"
     gene            complement(5257..5796)
                     /locus_tag="P5236_00665"
     CDS             complement(5257..5796)
                     /locus_tag="P5236_00665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRU62911.1"
                     /translation="MNIAVFCSSSNHIATYYHQVAFRLGEYIALKGNTLVYGGANGGL
                     MDAVAQGAHSKGGKIIGVIPQSILKMNRQSSLPMEFITTLNLSERKQIIKEISHLFIV
                     LPGGYGTLDEMFDVIASGITGEHHKPLIIVNEQHFFDLLIEQIKRMKNEFFIPENESY
                     QPYMVENLEECIAIIDSIY"
     gene            complement(5841..7799)
                     /gene="gyrB"
                     /locus_tag="P5236_00670"
     CDS             complement(5841..7799)
                     /gene="gyrB"
                     /locus_tag="P5236_00670"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HRU62912.1"
                     /translation="MINNEKLDAINEYGASSIQVLEGLEAVRKRPAMYIGDTSLKGLH
                     HLVYEVVDNSIDEAMAGHCDNIQVIINEDNSITVIDNGRGIPVDYHEKEGKSALEVVM
                     TVLHAGGKFDKGTYKVSGGLHGVGVSCVNALSSFMRVEVARNGKLYVQEYECGIPKFS
                     VKEIGESDITGTTVTFLPDKSIFTETVFKYDIIADRLRELAFLNAGIKLTLVDKRTIE
                     EDGNYKTEVFYSKEGLKEFVKYIDEAREKLIEDIIYINTEKNGIPVEIAITYNTSYNE
                     NIHSYVNNINTIEGGTHLAGFRRGLTRTLKKYAEDSKMLEKQKIEISGDDFREGLTAV
                     ISIKVAEPQFEGQTKTKLGNNEVMGAVDIAISEALGNYLEENPKSARIIVEKVILAAT
                     ARHAARKARELVQRKSPLSGGGLPGKLADCVEKDPAKCELFLVEGDSAGGTAKQGRSR
                     QFQAILPLRGKILNVEKAMQHKVLESEEIRNIYTALGVTIGTEEDSKALNLSKLRYHK
                     IIIMTDADVDGSHIATLIMTFFFRHMKEIIEQGYLYIATPPLYLCRKGKIEEYCWTEQ
                     QRQAFIEKYGNGQDGSVHVQRYKGLGEMNETQLWETTMNPEKRTLRQVTIENAASADH
                     VFSMLMGDDVLPRRQFIEEHAIYANIDA"
BASE COUNT         2600 a         1508 c         1291 g         2859 t
ORIGIN      
        1 tgctttgaga aaaaaggagt tttgttgaat gaagaagatg aagaaagtgt cattgctgaa
       61 ttaaactctt cactttttga acaatatgta gctgaaaaag tgattcaagg cggaatgata
      121 cccaaattac agaattcatt tgatgctctc aaagctggtg ttaaagaagt ttggattaca
      181 cgtgccgacc agttaaatag gaaaaagggg accaaaatta ctttataaat aaagaaattc
      241 cttaaaattg attatcctcg ccgcaagcag cgaggtatta cgccaatttt atggcttcgc
      301 caccggaatt tctaaaaaaa taaaaacaaa agcttccccg acgcaagcat cggggaattc
      361 ttacgattaa aatttgactt tataaaattg ataatcggtt gaaaacaatt aaaaaatcgt
      421 aaaaatagat gatagttaag aaattttata gaaattcttt attatggttg atactttaac
      481 aaacgaagca gtcgaacttt tacagaaaat gatttctatt ccttctttca gccgagaaga
      541 aaaagaattg gctgattttt tggaatttta tcttcaagca aaaggttatg atgtttttcg
      601 caataagaac aatatatggt taatcagtcc aggtttttct cctttacgac cgactttact
      661 gctaaattca catattgaca ctgtaagacc tgccaacgat tggactttaa atcctttcga
      721 accaaaaatt gagaacggaa aattatatgg tttaggaagc aacgatgccg gagcaagcgt
      781 ggtgagtttg ttgtctgctt tcttatattt aattcaacaa aatcaatctt acaatttaat
      841 ttttgctgct acagcagaag aagaaatttc tggagagaat ggaattgaat gtttgttaga
      901 ggaatttcct cctattgact ttgccattgt gggtgaaccc actggaatga aaatggctgt
      961 agctgaaaga gggttaatgg tacttgattg catagcaaga ggaaaatcgg cacatgccgg
     1021 tagagaagaa ggcgaaaatg ctatttacaa agctatagag gatatagaat ggtttagaac
     1081 ttatcaattc ccgaaacaat ctgattttct tggtgcagtg aaaatgacag tcacgcaaat
     1141 tgctggtggt acacagcaca atgtaattcc cgacacatgt acttttgtgg tggatgtaag
     1201 aagtaatgaa ctttatacga atgaagaaat tctggaaata attcgtgaac acgttcattg
     1261 tgaagtagaa ccgcgttcaa ttcgtttgtg ctcaagtaac gtgcaattag atcatcctat
     1321 tattaccaga gcacgagaac tacatcttga tttgtttggc tcccctaccc tttccgacca
     1381 ggctttaatg cctttcccaa gtgtcaaaat tggtccaggc gattctgctc gctcccacac
     1441 agccaatgaa tttattgaac tgaacgaaat tgaaaaaggt attcaaattt atattcagtt
     1501 actgaatggg ctcaagtggt aaggaagtaa tttctattta aaatcgataa tgttatgaat
     1561 ggttatcact ccgattacct tatctgaatc aggtgtttcc ataacagcaa gattggtaat
     1621 attgttctcg tccattattt ctaatgcttc actcagcaaa gcttcttgag taatccgctt
     1681 aaagccgtgt gtcataaaat cggctgctgt taagtgcttg atattatcaa atttctgaat
     1741 agcacgacga atatcgccat cggtaataat accgacagct tcttcatttc cattatacac
     1801 catagtcata cctaatcgtt tgttgctcac ttcgtaaatg acatccttga agggggtttg
     1861 tgtataaact tttggaatat cagtaaccat ttcatctttt acttgtgcta ataattgacg
     1921 ccccaatgct ccacctggat gataaagtgc aaaattctca gctttaaaat ttcttcgctc
     1981 cattaagcaa atagttaaag catctcccat taataaagtg gcggtggtag aagttgtcgg
     2041 agcaagtccc aaaggacaag cttcacgttc tacacctaca tttaatatta gtgaagcttc
     2101 tttggcaaga ggcgattttg ggttgccagt catggcaata atcgtgcaac caatattttt
     2161 taaaggaaaa attacattta ggatttcact ggaacttcca ctgtttgaaa tgagtaaaac
     2221 tatatcattt ttacttacca ttcccaaatc cccatgaaca gcttccgcag cattgataaa
     2281 aattgttggt gttcctgtcg aagctaatgt tgatgctatt ttatgtccaa tgataccggt
     2341 tttcccaata cccataatga ctaatttccc tttacagttg taaatcattt caactgcttc
     2401 atttatttca ggtcccaatc ggttagctaa cttttgaagt tcagtaattt ccagagcaaa
     2461 tatttcttta cttcgttcaa caaaatccat aatattcagc aataatattt cataattagt
     2521 tcaacaaatt ctctaataca accttcacca ccttttttct tcatttgtaa tattccaggt
     2581 atgtttttta ctgcttgttg tgcatcagca gggcaagctg ctaatcctac attagaaagt
     2641 aattcgacac aattcacatc atccccaata taagcaactt cgtccagtgt aattccttcc
     2701 ttttcacaaa tttcttttgc tgaagctaat ttgccacctt ctcgattacc ttgatagaga
     2761 taatccaatt ttagtttttc aaaacgacgt tcaacaattt tagtattttc cgaagtgatg
     2821 ataccaacct ttattccttg cttctgaatg agttttaaac ccattccatc gcttacattg
     2881 aattttttaa gttcatcgcc taattcagaa taatacattc cgccatcggt catcacacca
     2941 tccacatcac aaagaaataa tttgattttg tgacagaaat tttgtgaaaa agttaaatct
     3001 gtttcagcag aaatattatt aattttaaat gaagaatttt ttatcagatt atcaatttgc
     3061 aaagcttctt ccagaatttt tttgacactg gcgagtacta attgattcgg tccatcagaa
     3121 atggcataat ccggagaagg atgtacctca ataaaaatgg catcaacccc gacagccaat
     3181 gcagctcgca tcagataagg aaccatttca cgattgccac cggtagaagt tccttgtcca
     3241 ccaggtttct gaacagaatg agtggcatca aacaccacag gataaccata tcgtctcatt
     3301 tcaaccaatc ctgtcatatc aacaacaaga ttattgtaac caaaacatga acctcgttca
     3361 caaagaagaa tattttcatt acctgcttca ctgatttttt caataacgtt tttcatgtcc
     3421 cacggagcca taaactgacc ttttttgaca ttgataattt tccctgtttt agctgctgca
     3481 atcagtaaat cggtttgtct tgacagaaaa gcaggaattt gaagcacatc agccactttt
     3541 gcaaccactt cacattgcca tgtctcatga acatcggtaa gaataggcaa atcgaaatct
     3601 tccttaattt gctgaagaat ttgaagacct cgttccatac ctattcctcg tgaagatttt
     3661 attgaagtcc ggttggcttt atcaaaagaa gatttaaaaa taagatggat atttagttct
     3721 tcacttattt gttttaaggt tgaagctgtt tctctcgtaa ttttttcgtt ttctatggca
     3781 cagggacctg caatgagagt aaaacgttga ttccctccaa tggtaaaacg gttcagaaca
     3841 tttatttctc tggtgggaag tatcattatt aaacaattaa gataaaatgc acaaataatg
     3901 cgacaaagat agtattattc ttttttaaat tgatatactt ttacccaatc aacttctaaa
     3961 attccttcgt ctcctttttc tttttccgga ataaatgaat taaatgctaa atacattttt
     4021 tcttgaggta agtttgaaga agtacgataa acttccctat catttataaa ccagatcaat
     4081 tcattgtctg tccatacaag cgtgtagata taaaattgtg aaggatgaat acctttaatt
     4141 ttattcccat caaatccact ttcatgggca ttccccacgt taatattttt cccatcaaaa
     4201 tgaaagatgg taatttgagg aagttttcta tccgttccta accaaaaagc atgatgaatt
     4261 tttcctgagc agcgaagttt ggcacgaaat actcctcgtt gttgtcgaaa aaaatcggct
     4321 gtttgaataa tgtctgaggt atattcaaaa tcttttaata tgaatccttt tacaggatcc
     4381 caagctcgag cagttacttt ttctttttta gtataaattt tcaagatgcc attttccacc
     4441 actacgtttt tccctttatt gtaagcttgt ttttcattgg caaaagaatg atcttgaatt
     4501 agaacgggat gttgataatg aaagccgtaa gaccatcgtg atctatccaa tgtatgccaa
     4561 tcaaaatttt cttcgaaagt aagttttaaa tttcgatatt tttcaaaacg ttcaggtttc
     4621 tcattttcat aataaacgat atcgggattt ttacgtagtt gatgataacg ttgctgtaag
     4681 gcatattctg gagtggcatg ccaccgattt gcatttgccc agaattcatt cgattcttta
     4741 aattccggca aattgacttt ttccttcaac tcttcatatt gtttgataat ataagaatcg
     4801 tgaaaacgaa tataattttt gtaagctttt gaatgttcaa attctttgaa tttttgaaga
     4861 gttttggaag catttacttt ctttttatct cccaaatctt catattctga cgttttcttg
     4921 aattgaagat attcctgcaa gagtggagat tgtaaagttt cataatataa acgaatagct
     4981 ggtgattttt ggagtttacg gtattgtgta acgatttgat attcttctgt atcttgatat
     5041 ttgcgattct ttaataattt ctttttttct tgaaactcag atgaacttat tgtatgaaat
     5101 aaatttttgt attcttcaag ttctgcagat ctactaatct caaaataacg ttgcatatct
     5161 ttcaacaacg ttgcttcatt tttttcaaat ttagaagtag aagtaatttt tttaagcggt
     5221 tttttttgaa actgattcat gaatttagtt tttaagttaa tagattgagt ctattatagc
     5281 tatacattct tcaagattct ctaccatata aggttgataa ctttcatttt ccggaataaa
     5341 aaattcattt ttcattcgct tgatttgttc aataagaaga tcaaaaaagt gttgttcatt
     5401 aacgattatt aaaggtttat ggtgttctcc tgtaattccc gaagcaatta catcaaacat
     5461 ttcatcgagc gttccgtatc ccccaggaag gacaatgaat aaatgagata tttctttgat
     5521 aatctgtttt ctttcgctta aatttaatgt ggtaataaat tccattggta gggaactttg
     5581 ccgattcatt ttaagaatgg attgtggaat tactccgata atttttccgc ctttagaatg
     5641 agctccttgc gctaccgcat ccatcaatcc gccatttgct cctccataaa caagcgtatt
     5701 cccttttaaa gcaatgtatt ctcctaaacg gaaagcaact tgatgataat aggtagcgat
     5761 atgattactc gaggaacaaa aaacggcaat attcatttat tcgtttttgt tgaagaataa
     5821 attaattctg tttttataaa ttaagcatca atattggcat aaatggcatg ttcttcaata
     5881 aactgacgac gaggaagcac atcatctccc attaacattg aaaatacatg gtcggctgaa
     5941 gctgcattct caatagtaac ttgacgtaaa gttctttttt caggattcat tgttgtttcc
     6001 cataattgag tttcattcat ttcacctaaa cctttatagc gttgaacgtg tacagaacca
     6061 tcctgaccat ttccatattt ttcaataaaa gcttgacgtt gttgttctgt ccaacaatat
     6121 tcttcaattt tcccttttct acataaataa agcggaggag tagcaatata cagataccct
     6181 tgctcaatta tttctttcat atgtctgaaa aagaaagtca taataagcgt agcaatatgg
     6241 ctaccatcca catctgcatc tgtcataata attattttat gataacgcag tttacttaaa
     6301 tttagtgcct tactatcttc ttctgttcct atagtaacgc ctaaagctgt atatatattg
     6361 cgtatctctt cactttccag cactttgtgt tgcatggctt tttctacatt taaaattttc
     6421 ccacgcaacg gcaatatagc ttgaaattga cgactacgac cttgttttgc agttccacct
     6481 gctgaatctc cttcaacaag aaataattca cattttgcag gatctttttc aacacaatcg
     6541 gcaagtttac cgggcaatcc accacctgaa agaggtgatt tccgttgtac caattcacga
     6601 gctttacgtg ctgcgtgacg agcggtggcg gctaaaatta ccttttcaac aattatgcga
     6661 gctgatttag gattttcttc caagtaattt cccaaagctt cactgatagc aatatctaca
     6721 gcacccatta cttcattatt ccctaatttg gttttggttt gtccttcaaa ttgtggctct
     6781 gccactttaa tagaaattac agcagttaaa ccttcacgaa aatcatctcc gctaatttca
     6841 attttttgct tttctaacat tttactgtct tcagcatatt ttttcaaggt acgcgttaaa
     6901 ccacgacgaa atcctgccaa atgagttcct ccttcaatag tattgatatt attaacatag
     6961 gaatgaatat tttcattgta agaggtatta taggttatgg caatttctac tggaattcca
     7021 tttttttcag tattgatata tataatatct tcgataagtt tttcacgagc ctcgtcaata
     7081 tatttgacaa attctttcaa tccttctttt gaatagaaaa cttctgtttt gtagttccca
     7141 tcttcttcta tcgtacgttt atccaccaac gttaatttaa taccagcatt cagaaaagct
     7201 aattctctta accgatcggc aataatgtca tatttgaata cagtttcagt aaatatactt
     7261 ttgtcaggca ggaaagtaac agtcgtgcct gtgatatctg attctcctat ttcttttact
     7321 gaaaattttg gaataccaca ttcgtattcc tgcacataaa gttttccatt gcgagccact
     7381 tccactcgca taaaagaaga aagggcatta acgcaagaaa cgcctactcc atgcaatcca
     7441 cctgaaactt tgtaagtacc cttatcaaat ttaccaccag catgaagaac tgtcattact
     7501 acttcaagag ctgatttccc ttctttttca tgataatcca ccggaatacc acgcccatta
     7561 tcaataactg tgatagaatt atcttcatta ataatcacct gaatattatc acagtgacca
     7621 gccattgctt cgtcaataga attatctact acttcatata ctaaatgatg taaaccttta
     7681 agtgatgtgt cgcctatgta catggcaggt cgcttacgca cagcttctaa tccttctaaa
     7741 acttgaatgc tactcgcacc atattcattg atagcatcta atttctcgtt gtttatcatt
     7801 tttattttga gtttaaattg atttaatttt ttaatccttt taaaaatcat acaaatttaa
     7861 ggaaaaaaat tgaaatgacc taactttttg tcggaaaaat gacctttcac aaatgaacaa
     7921 attaattgaa aatcagaaaa atatattaaa ttttattaat aataatagta agaaaatcga
     7981 aattttaaat ggggtgcaat aaaaaaaaaa ttttataaaa tttgattttc ttttttattc
     8041 aaatcatgtg tgaaaataaa ttatacctac catagaaagg aaaaaccatt aaagataaac
     8101 aaatttttag ttgtttacaa cataaattat tgataatcaa atcatcaagt tgttattaag
     8161 tacacacttt gaaattttta tgcgtcgact ttgtaatatt cttataattc tttggattta
     8221 aataaaatat tttgtatttt tgcatcgaat tattgaat
//
LOCUS       DAOTKS010000015         8256 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_8685_length_8256_cov_6.693818, whole genome shotgun sequence.
ACCESSION   DAOTKS010000015 DAOTKS010000000
VERSION     DAOTKS010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8256
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8685_length_8256_cov_6.693818"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..163)
                     /locus_tag="P5236_00675"
     CDS             complement(<1..163)
                     /locus_tag="P5236_00675"
                     /inference="COORDINATES: protein motif:HMM:NF013068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRU62913.1"
                     /translation="MVHQIRNACKYVVWKDRKEFTSDMKNIYTAPNRQAAEAALNDFA
                     KNRKQNMLIL"
     gene            complement(464..610)
                     /locus_tag="P5236_00680"
     CDS             complement(464..610)
                     /locus_tag="P5236_00680"
                     /inference="COORDINATES: protein motif:HMM:NF013068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRU62914.1"
                     /translation="MENIIISFYAKGMTVSDMKNRFEKCMVFEVSTATISRITNAVTE
                     EATG"
     gene            complement(898..1296)
                     /gene="ssrA"
                     /locus_tag="P5236_00685"
     tmRNA           complement(898..1296)
                     /gene="ssrA"
                     /locus_tag="P5236_00685"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1366..2886)
                     /gene="rny"
                     /locus_tag="P5236_00690"
     CDS             complement(1366..2886)
                     /gene="rny"
                     /locus_tag="P5236_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HRU62915.1"
                     /translation="MELIIGIIIALAVGVTGTFLYFKQNNKRMLKKMEEEAELVKKNN
                     LIEVKEKFIALKSEYEQQVQQRNAKLQASEVKLQQREMQLNQRQGELQRKMNEIESMK
                     ENLESQFIATENRKKELEHLIRQAQEQLETISGLSTEQAKEQLIESLKEEAKTEAMSY
                     VNDIVDEAKMTANKEAQKIIIQTIQRVATETAIENSVTVFHIESDEMKGRIIGREGRN
                     IRALEAATGVEIIVDDTPEAIVLSAFDPVRREIARLSLHQLVADGRIHPARIEEVVNK
                     VKKQLEDEIIEVGKRTTIDLGIHGLHPELIRLIGKMKYRSSYGQNLLQHARETANLCA
                     IMAAELGLNPKKAKRAGLLHDIGKVPDDEPELPHAILGMKLAEKYKEKPDICNAIGAH
                     HDEIEMETLIAPIVQVCDAISGARPGARREIVEAYIKRLKDLEEVALSYPGVVKTYAI
                     QAGRELRVIVGADKMDDKEAELLSMDIAKRIQDEMTYPGQIKITVIRETRAVSYAK"
     gene            complement(3056..3349)
                     /locus_tag="P5236_00695"
     CDS             complement(3056..3349)
                     /locus_tag="P5236_00695"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HRU62916.1"
                     /translation="MDNDTFLINVQAGGFKLPLRIARKDEEIYRKAEKLFSKYLEKYQ
                     QKYHQRANEEILTLVAYQFAVLLIKQEMRQDVSPVIEKLTSLNKEIENCLSRK"
     gene            complement(3360..3563)
                     /locus_tag="P5236_00700"
     CDS             complement(3360..3563)
                     /locus_tag="P5236_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU62917.1"
                     /translation="MLKKEIELKDNELKSLQQKVLELQEKINHFQIAKALAATPEESS
                     LAKQQLSKMVREIDKCLALLLKE"
     assembly_gap    3750..3849
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3850..5549
                     /gene="ispG"
                     /locus_tag="P5236_00705"
     CDS             <3850..5549
                     /gene="ispG"
                     /locus_tag="P5236_00705"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HRU62918.1"
                     /translation="PIRLQSMTNTDTNDIDASVSQCIRLIKVGAELVRLTTQGLREVE
                     SLKKIHQQLNEKKFYVPLIADVHFNPLVAEAAAQCVEKVRINPGNYVKSIKINHSADY
                     TEEEFNLEQQKLKEQFIRLINICKEHRTAIRIGVNHGSLSERMMNRYGDTPEGLVASC
                     MEFLQICKEENFSDVVVSIKASNTVLMIHAVRKLVKEMEQKGFPYPIHLGVTEAGEGE
                     DGRIKSAVGIGTLLADGIGDTIRVSLSEEPEAEIPVARQLVSYIAEREQHEKIFAQIN
                     PSWSPFEYSCRKTQAVANIGGNHLPVVFATTQKNFTISPDYYIEGRKIYNNAGKIFSV
                     YLPDEIPLLKKDVSEVKFLKLSYPELNSDVLSFLKERKDVVILLFTKHKNGAAEQRAF
                     FHTLMNKDITTPVIIARNYQENNLESLQIKASVDIGMLFVDGFGDGILLQNEGTISLK
                     DINSLSFGILQATRVRISKTEYISCPGCGRTLFDLQKVVAEIKAKTSHLKGLKIAVMG
                     CIVNGPGEMADADYGYVGTGKGKVSLYKGKQCLLKNIAEDEAINKLIELIKVNGDWL"
     gene            5646..6116
                     /locus_tag="P5236_00710"
     CDS             5646..6116
                     /locus_tag="P5236_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU62919.1"
                     /translation="MKKTIFIAILLVSFMTSFAYGQVNSKAIGVRASYGAELSYQHPF
                     SHANRLEADLGFYYDHGFNLAGVYQWVFDLSELANGFNWYVGLGPQIGSWDYHHNGKD
                     YDGLALGIAGQIGIEYNFDIPLQLSLDYRPGWYILPNSYGGAWEGVALGIRYKF"
     gene            complement(6204..7910)
                     /locus_tag="P5236_00715"
     CDS             complement(6204..7910)
                     /locus_tag="P5236_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRU62920.1"
                     /translation="MKKQLFFALSMVVAIILCSCSKDMGALDPTSFKCTPNPLEAKGG
                     KINATINGTFPVKYFNKNAVVTVTPVLRFQGQEVKSTPATFQGEKVIGNNKVISYKAG
                     GSYTINASFNYVPEMAKSELYLDFDVTVKNKNYQIPSVKVADGVIATSELAVVNANEI
                     QGALIPDKFQRIIKETQDADIKFLIERWDLRPTELKKEEVVSLVNKIAEVKKDTNKKV
                     SDFEIAGYASPDGPIDLNTNLAEKRQKVSVDYINNQMKKLKTSVKIDSKFTPEDWEGF
                     QKLMENSNIQDKEVILRVLSMYSDPEERERQIKNLSAAYQKIAEEILPQLRRSRLKLT
                     VDIIGKSDDQISQLAANNPSQLSVEELLYAATLKNNLNDKAIIYQEVINLYPNDIRGY
                     NNLGLIYYQQDKIDEAEKLFTQALQKDPNSPDVNFNLGLIALKKGDVTKAEELFGKAA
                     NTSGELSKALGTLYLKKGDYAKAMNSFKSNDISNNVALLYILNNDYNQARKVLAAIPQ
                     PDATTAYLSAIVGARTNDRDAVYSNLRTAISKDKSLAQKALNDIEFAKFATDSTFLSI
                     VK"
BASE COUNT         2497 a         1445 c         1361 g         2853 t
ORIGIN      
        1 acagtataag catattttgc ttcctatttt ttgcaaaatc attcaatgca gcttcagcag
       61 cctgtctgtt gggagcagtg taaatgtttt tcatatcaga ggtaaattct tttctatctt
      121 tccagacaac atatttgcaa gcatttcgaa tctgatgaac cacataaatc tgggtttgaa
      181 actgtggaaa aaccgaacga atagtttggt aaaaccgttc aaattatccg ttacggtaat
      241 aggaatatct tctatgctgc aagttttcag atccgtgaga acactcatcc agaaagcagc
      301 gttttaattt cgtcccagcc acacaccaac acttctttcc ttctttcaca gtttaatcct
      361 actgcaaggg aaatcgtttt attgataatt cgggaatttc cccaaacttt aaacataatg
      421 ccatccatcc atacaattaa ataaattcca gcggacgatt ctgtcagcct gttgcttctt
      481 ctgtcacggc attggttatt cgggatatgg tggctgttga tacctcgaaa accatacatt
      541 tctcgaatct gttcttcata tcgctcaccg tcattccttt ggcataaaac gagataataa
      601 tgttttccag accttcgata atgttatacc ttttaggaac taaaacaggt tcaaaactgc
      661 cttcttgtgt agttctttaa agaatgagct catttcttct ccattcttga agaattttaa
      721 aaaatccttg ttgtttaatc aaaagaattc ctcgaggctt gcctcggggt tattcattga
      781 tgtgaatgat acgattgttt tttgtaaaat taaaaaaatc agtagtgaat ttatgaattt
      841 tttctataac ataaacatta aagtaaaatt ttcttaagaa gaaatggcaa gattaaaaag
      901 tggagctgga gggatttgaa ccctcgtcca aacaaggaac cgatatgttt tctacatgct
      961 tagcttcgct tgatttttcg tgtacaggca aggccgaagc tgccgacctg taccttatcc
     1021 cttatgattt tcattgcgtt tacaaggcta aacgcaacta tcctcgattt cgctgcacct
     1081 ccacatcagg ctgcctcgag gagagggcgc ctgggagatg tctcgtcccc acaccttgtg
     1141 cagggataaa gcttaatcta ctttacttcg attaagcagc gagagcgtaa ttagtttcgc
     1201 cagttacttt ttgagatccg agatttacga gctgtgacct cccggctcgg catgcttaca
     1261 taccctttca acttgctgtc aaaaccaaac agccccataa aaggatatat attcaaaata
     1321 aatttacaac gctttgtaaa tcactcattc actcttcaga caattttatt ttgcataact
     1381 tacagccctc gtttcacgaa tgactgtaat ttttatttga ccaggataag tcatttcatc
     1441 ctgaatcctt tttgcaatat ccattgagag caattctgct tctttatcgt ccatcttgtc
     1501 agcacctaca attaccctca gctctcttcc tgcctgaatg gcataagttt ttactactcc
     1561 aggatacgaa agagcaactt cctccaggtc tttcaatcgt ttaatgtatg cttccacaat
     1621 ttcacgtctc gctcccggcc gtgctcctga tatagcgtca caaacctgta caatcggagc
     1681 aattagtgtt tccatttcta tttcatcatg gtgtgcacca attgcattac aaatatcagg
     1741 tttttcctta tatttctcag caagtttcat accaagaata gcatgtggca gttcgggttc
     1801 atcatcgggt actttaccaa tatcgtgtaa taaaccagcc ctttttgctt ttttaggatt
     1861 caatcctaat tctgctgcca tgatcgcaca aagattagca gtttcgcgtg catgttgcaa
     1921 caaattttga ccatacgaag agcgatattt cattttccca ataagacgaa ttaattcagg
     1981 atgcaatcca tgaattccta aatcaatggt cgtacgttta cccacttcaa tgatttcatc
     2041 ttccagttgt tttttcacct tattgaccac ctcttcaatg cgagctggat ggattcgtcc
     2101 atcagccacc agttgatgta gcgacaatcg ggcaatttca cgtcgaacag gatcgaaagc
     2161 tgaaagaaca attgcttcag gtgtgtcgtc cacaataatt tcgaccccag tagctgcttc
     2221 cagtgctcga atattacgac cttcacgacc aataattcgt cccttcattt cgtcggattc
     2281 aatatggaaa acagtaacag aattttcgat agccgtttca gtagccacac gctggatggt
     2341 ttgaatgata atcttttgag cttctttatt agcagtcatt tttgcttcat ctacaatgtc
     2401 attgacataa gacatggctt ctgttttggc ttcttctttt agtgattcaa ttaattgttc
     2461 tttcgcttgc tccgttgaaa gacctgaaat agtttccaac tgttcttgtg cttgccgaat
     2521 aagatgctca agctcttttt ttcgattttc ggttgctatg aattgacttt ccagattttc
     2581 cttcatcgat tcaatttcat tcatctttct ttgcagttca ccttgccgtt ggttgagttg
     2641 catttctctt tgctgcaatt ttacttcgct ggcttgcaat tttgcatttc gttgctgaac
     2701 ttgttgttca tattccgatt tcagagcaat aaatttctct tttacttcga ttaaattgtt
     2761 ttttttgacg agttcagctt cctcttccat ttttttaagc atacgtttat tgttctgctt
     2821 gaaatatagg aaggtacccg tcacacccac tgctaatgca ataataattc ctatgatgag
     2881 ttccattttt tgattttatt atttagtaaa atttgataaa tagaaatata taaatatata
     2941 aatataaaaa aacgcattca cgttgtttaa ataatcattt ttattggagt ttcactccaa
     3001 taaaacaacg caatgcggga aatttagttt aggaaaacga taaaaatttt actgattatt
     3061 ttctacttaa gcaattttca atttctttat tcaatgatgt taatttttca atcacaggag
     3121 aaacgtcctg cctcatttct tgctttatta gtaaaactgc aaattgataa gcaactaaag
     3181 ttaaaatctc ttcattggct cgctgatgat atttttgctg atatttttca agatattttg
     3241 aaaataattt ttcagctttt cggtatattt cttcatcttt tcgggcaata cgaaggggta
     3301 atttgaaacc acctgcttgt acatttatta aaaaagtatc gttgtccatc atcttgctct
     3361 tattctttta gtaaaagagc taaacatttg tcaatttccc gcaccatttt actaagctgc
     3421 tgctttgcca agctactttc ttcgggcgta gctgctaatg ctttagcaat ttgaaaatgg
     3481 ttaatttttt cttgtagttc caaaactttt tgttgaagag attttaattc gttgtctttt
     3541 aactctattt cttttttcaa cataaagttc tgtactttta atgactcgta ctccgaaatt
     3601 aattttcgca gattcgaagc aaatacgttt attagttcct cgtactgatt agtcatttta
     3661 caaaaaaatc tatacaaagt tacacaatca catttaatta ataaaattta ttgcttattt
     3721 ttcattttgt ttaaatattt gcttaaaaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnc acctattcgt ttgcaatcga tgactaatac tgataccaac gatatcgatg
     3901 cttctgtatc gcaatgtatt cgtttgatca aggttggtgc agaactggtg cgacttacta
     3961 cacagggatt acgcgaagta gaaagtttaa aaaaaattca tcagcaatta aatgaaaaaa
     4021 aattttatgt cccgttaata gcagatgtcc attttaatcc tctggtagca gaagctgcag
     4081 ctcagtgtgt agaaaaagta cggattaatc caggtaatta cgtgaaaagt attaaaataa
     4141 atcattcagc ggactatacg gaagaagagt ttaatttaga acagcaaaaa ttaaaagaac
     4201 aattcatacg gcttatcaat atttgtaaag aacatcgcac tgctattcgt attggagtga
     4261 atcatggttc cttgagtgaa agaatgatga atcgttatgg cgatactccc gaaggattag
     4321 ttgcctcctg catggaattt ttacaaattt gcaaggaaga aaatttttct gatgtagttg
     4381 tttctataaa agcttccaat actgttttaa tgatacacgc tgtgcgaaag cttgtaaaag
     4441 aaatggaaca gaaaggtttt ccttatccca ttcatttagg agtaacagaa gcaggggaag
     4501 gtgaagatgg tcgcatcaaa tcggcagttg gaatcggaac tttactggca gatggaattg
     4561 gtgataccat tcgtgtttct ttgagtgaag aaccagaagc cgaaattcct gttgcacggc
     4621 aacttgtatc ttatattgct gaacgcgaac aacatgaaaa aatttttgca cagattaatc
     4681 cttcatggtc acctttcgaa tattcttgta ggaaaaccca agctgttgct aatataggtg
     4741 gaaatcattt gccagtagta tttgctacta cacaaaaaaa tttcaccatt tcaccagatt
     4801 attacataga agggagaaaa atctataata atgctggaaa aattttctct gtttatttgc
     4861 cagatgaaat tcctttacta aaaaaagacg tatcagaagt aaaattttta aaattgtctt
     4921 atccagagtt gaattctgat gttttatcgt ttctgaaaga aagaaaagat gtagttattt
     4981 tgctttttac aaaacacaaa aatggagctg ctgaacaaag agcttttttc catactttga
     5041 tgaataaaga tataactact cccgtcataa tagctcgaaa ttatcaagaa aacaatttag
     5101 aaagtttaca aataaaggcg tcggttgata ttggtatgtt atttgtggac ggcttcggcg
     5161 acggaatttt gttgcaaaat gaaggtacaa tatccttaaa agatatcaat tcgctgtcat
     5221 ttggaatttt acaggctacg cgagtaagaa tttcaaaaac agaatatatt tcgtgcccgg
     5281 gctgtggacg caccttattt gacttgcaaa aagtagttgc agaaataaaa gctaaaactt
     5341 ctcatttaaa aggtttaaaa attgctgtaa tgggttgtat tgtgaatggt cctggagaaa
     5401 tggcagatgc cgattatggt tatgtcggta ctggaaaagg gaaagtaagt ttgtataaag
     5461 gcaagcaatg tttgttaaaa aatattgccg aggatgaagc tataaataaa ttaattgaac
     5521 tcataaaagt gaatggtgat tggctataaa aagactgaaa aaaataaaat attatctttt
     5581 ctaatttttt cttttaaaat aatgtgattc tcttaaaaat ggtttaacaa ttaaaaaatg
     5641 aaggtatgaa aaaaacaatt tttattgcga ttctattggt aagctttatg acgagctttg
     5701 cttatggaca ggtaaacagt aaagctattg gagttcgtgc tagttatggt gccgagttat
     5761 cttatcaaca tccttttagt catgctaatc gacttgaagc agacttaggt ttttattacg
     5821 atcacggttt caatcttgca ggcgtttatc aatgggtttt tgatttatca gaattagcaa
     5881 atggttttaa ttggtatgta ggtttaggac ctcaaattgg atcatgggat tatcatcata
     5941 atggtaaaga ttatgatggt ctggctttag gaatagcagg acagattggg attgaatata
     6001 attttgatat tcctttgcaa ctttcattgg attatcgtcc aggttggtat attttaccaa
     6061 atagttacgg aggagcctgg gaaggagtag ctttaggaat acgttataaa ttttagaggt
     6121 aagaaaaact tttttgaaat ggttgaatga aaaaagaggc agcttatttt agctgcctct
     6181 tttttagtat taataaagtt atcctacttt acaattgaca ggaaagtaga atcagtagca
     6241 aattttgcga attcaatatc attcaatgcc ttttgagcta atgatttatc tttgctaata
     6301 gctgttctta aatttgaata aacggcatca cgatcatttg ttctggctcc aacaatagct
     6361 gaaagataag ctgttgtagc atcaggttgt ggaatagcag ctaaaacttt tcgagcctgg
     6421 ttataatcat tattcaggat atacagcaaa gctacattgt ttgatatgtc attcgattta
     6481 aacgaattca ttgcttttgc ataatcacct tttttcaaat acaatgttcc taaagcttta
     6541 cttaattctc cacttgtatt ggctgctttt ccaaataatt cttcggcttt ggtcacatcg
     6601 cctttcttta atgcaatcaa tcctaaatta aagttaacat caggtgaatt tggatccttc
     6661 tgcagagctt gagtaaacaa tttttctgct tcatctatct tgtcttgttg atagtaaata
     6721 agacctaaat tattataccc acgaatatca tttggataaa ggtttattac ttcctgataa
     6781 ataattgctt tatcattcag attatttttt aaggtcgcag cataaagcaa ctcttcaaca
     6841 gaaagttgcg atggattgtt agcagctaat tgagagattt gatcatcaga tttaccaata
     6901 atatccaccg taagtttcag tcgagaacga cgtaattgag gaagaatctc ttctgcaatt
     6961 ttttgataag ctgcagatag atttttaatc tgacgttcac gttcttctgg atcagaatac
     7021 atcgacaaaa ctcttaaaat aacttcttta tcctgaatat tggaattttc cattaacttc
     7081 tggaatcctt cccagtcttc gggtgtaaac tttgaatcaa tttttacaga agttttaagc
     7141 ttcttcattt gattgttgat ataatcaaca gaaacctttt gacgtttttc agcgagattg
     7201 gtatttaaat caattggacc atcaggtgaa gcatatcccg ctatttcaaa atcagaaact
     7261 tttttgttcg tatctttttt tacttcagca attttattta ccaaagaaac cacttcttct
     7321 ttctttaatt cagttggacg aagatcccat ctctcaatca aaaatttaat atctgcatct
     7381 tgggtttcct taatgatacg ttgaaattta tcaggtatca aagcaccttg aatttcattt
     7441 gcattaacaa cagccagctc agaggttgct ataacaccat ctgcaacttt gacgcttgga
     7501 atctggtaat tcttgttttt aacagtgaca tcaaagtcaa gatataattc cgattttgcc
     7561 atttctggta cataattgaa agaagcatta atggtataag aaccaccagc cttgtaagaa
     7621 atgactttat tatttcctat tactttttcg ccctggaaag ttgcaggggt acttttcact
     7681 tcttgaccct gaaaacgtaa aacaggagtt accgttacta cggcattctt gttaaaatat
     7741 tttacaggga aagttccatt tattgtggca tttattttcc caccttttgc ttcgagagga
     7801 ttgggagtgc atttaaaact ggtaggatca agagctccca tatctttact gcacgagcaa
     7861 aggataatag ccaccaccat tgataaagca aaaaataatt gttttttcat acgacagaaa
     7921 tttttttaat gttaatgatt caattatatt taattctaca gaaatagaat aatactaact
     7981 tgacaaagct acaatttttt gttcaattat tctttatttt tgcaaaaaaa attcttaaac
     8041 ttttcttctt tctaaacttt ttaaaaataa ttcataaatg aattatcaaa aaatatacca
     8101 ctggttacat tctttaaaat accttaacat taccaagagt tttaatttta ttaaactctt
     8161 gatttcatat attttatcta tttttaaaat taataataca aaaaaaagaa atttaccttt
     8221 ttttgtttca atagaacctg tcgatttttg caatct
//
LOCUS       DAOTKS010000016         8241 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_8697_length_8241_cov_4.680064, whole genome shotgun sequence.
ACCESSION   DAOTKS010000016 DAOTKS010000000
VERSION     DAOTKS010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8241
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8697_length_8241_cov_4.680064"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="P5236_00720"
     CDS             <1..1000
                     /locus_tag="P5236_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRU62921.1"
                     /translation="AKAQTTAGWLSILFMAFTLVVVSFSCTGPIIGTLLVEVSSNQSI
                     LAPTIGMFGFALALAVPFTLFAFFPSWLKSLPRSGGWLNQVKVVLAFIELAFALKFLS
                     VADLAYGWHMLDREVFLSLWIVIFTLLGIYLLGKIRFPHDSEIKHVSVLAVFWAIISF
                     SFAIYMIPGLWGAPLKAISAFTPPLYTQDFNLYQQNIYPDFTDYDEGMTFAKEKNLPV
                     IVDFSGYGCVNCRKMEANVLSDPEISNFLKKQFVLITLYVDDKTPLQKPYEVIENGKV
                     RKIKTVGDKWSYLQRYKFGANAQPFFVMLNNDGKPLNKPFTFDDHPQHFLKWLTKK"
     gene            1211..1777
                     /locus_tag="P5236_00725"
     CDS             1211..1777
                     /locus_tag="P5236_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRU62922.1"
                     /translation="MEKGKDMANNEINEQEKISGKKENLVPQAEKTEENENIEKELER
                     LRIECEELADKNLRLIAEFDNYRKRMLKEKAELLKNASESVLVNLLPLVDDFERALQA
                     IETTDDINAIKEGVQLIYNKFINFLHQYNVKEIPTIHQEFDTQLHEAVSTIPAPNEDL
                     KGKIVDCVLKGYTLNDKVIRHAKVIVGE"
     gene            1838..3004
                     /gene="dnaJ"
                     /locus_tag="P5236_00730"
     CDS             1838..3004
                     /gene="dnaJ"
                     /locus_tag="P5236_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRU62923.1"
                     /translation="MAKRDYYEILGISRTATEVEIKKAYRQKAIQYHPDKNPGDKQAE
                     ENFKEAAEAYEVLSDPEKRDRYDRYGHSGVSGSGGGGFGQGMTIEDIFSHFGDIFGGH
                     FGGFSGFGGFSGFRSDSQSPNTKIRHGSDLRIKVKLTLEEIASGVEKKVKVKKYVACP
                     SCKGTGAAEGSSPVTCSTCRGTGRVTRVQQTILGRMQTTVECHTCKGEGKIIVNKCPY
                     CNGEGIIQDEEIITINIPAGVSEGMQLTFAGKGNAARRGGINGDLIVLIEEEPHPSLI
                     RDGNDLIYNLLLSVPQAALGGSIEIPSLTGKVKVNIEPGTQPGKVLRLKGKGLPDVNR
                     YGNGDLLINIGVYIPEKLSKEEKNLLEKLDKSNNFKPNPAASKKFFSHFRNMFE"
     gene            complement(3276..3689)
                     /locus_tag="P5236_00735"
     CDS             complement(3276..3689)
                     /locus_tag="P5236_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU62924.1"
                     /translation="MWIFLIIAIILGVILFAFAHFNKKKATNNHLINPSATCSYCDAY
                     NECQVKQLFNKENEIIYYDDEELDALKGIPANEYTQEQIKQISEVFHTLQKNEVKDWL
                     NSLQLRNIELPLELRDEIFSQLEEKNFSCEYLQSQ"
     gene            3937..>5171
                     /locus_tag="P5236_00740"
     CDS             3937..>5171
                     /locus_tag="P5236_00740"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRU62925.1"
                     /translation="MLLLLMLLVGSCSLQKNTKFSRSYHALTTRFNVYFNAEQSYQEG
                     LKNIWDANKDDYSTVLPLYPISRHSNAAAATANMDRTIEKCRKAIKLHSIKQKPEKDY
                     KKWNSPEYQAFYNQEEFNPLLKEAWLLLGKAEFHKGDFLGSIGTFSYIAHHFAADPDV
                     IIQCQLWMARAYAEMGWLYEAEDVLSKINQDQLKKEHVGLFAAVNADILLKKKEYKEA
                     IPYLQLAIEKESDKKLKQRFTFILGQLYQITGNENAAYQAFTQVLKSNPPFEMDFYAR
                     IQRAQLNASNFSLTQKELTKMLKNSKYKDYQDQIYFAMGKNLLMKGDTLKAIENFKSA
                     IDKSTRNSYDKANALITLGDLYYKNKNYKQAYPCYEEASKIITNEHEDYQRVSRLSEI
                     LSEWVVQYDIVMLQDSLQR"
     assembly_gap    5172..5271
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5272..6770
                     /locus_tag="P5236_00745"
     CDS             <5272..6770
                     /locus_tag="P5236_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU62926.1"
                     /translation="SEAYFQLYLMESKMGNRDKAEQWRTKLITEFPETTFARLLSKPD
                     YPERLKRMYQEQDSIYRLAYQAYTKNDFNAVFQYKEYMEQNYPTSPLMPKFLFLEALS
                     IGKTSSSERFEKELSNLISAYPESDVSSMAKDILALIKQGKQVQTSSTHGSLLAKREE
                     IIKTEKETVVQQQFSPDKKTKHLLLLISDADKRDLNQLMYQLASFNFSRFLIKDFDIG
                     IQPLDSTRNILFVANFDSYDEVLWYLNTLSSDELLQSLMNQLNIQKVIISENNFALLK
                     TTFSLNDYLAFHDKFLTPDNKAIKEITASFEKKTASTVIPSSAAGKPQIGGETQSPAK
                     ALKNENVLTQSVAPTLPLEKQTEAALPSTTPPITKEEAPLYKGLFAYKPNELHFVTIY
                     VVSGNVDETKVQNAFDKYNQQNYGILNLSTRAEKVGKQQFFIIGPFPDANTAKSYLMR
                     IVKESSLFEGLKGANYRNLVGTQENLNTMLQQNALNVYFDFMKEYYLK"
     gene            6808..>8241
                     /locus_tag="P5236_00750"
     CDS             6808..>8241
                     /locus_tag="P5236_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HRU62927.1"
                     /translation="MKQGKILWIDDEIDLLQPYLIFLNDKGYEVLTATNGNDAIEICQ
                     QENFDIIFLDENMPGLTGLDTLAVIKELLPDVPVVMITKNEEENIMNMAIGNKIADYL
                     TKPVNPSQILLTLKKNIHIKEIVTEHATSTYRSEFSQIGMKINDSLTFEEWIDIYKKL
                     VYWELELSETDSGMDEMLQMQKTEANTAFAKFIKTNYLKWFENPETRPLISPDLFKTK
                     IFPLLDKGEKIFFVVVDNFRYDQWKIIRDLLGDFYQFEEEELYCSILPTATQYARNAI
                     FSGLMPLQIEQMFPDLWVEEIDEEGKNLKEKDLLATQIQRFRKNYEFSYHKINDSNSC
                     QRLIDQLPRLENCQLNVCVLNFIDMLSHARTDSKMVRELANDEKAYRSLTLSWFKYST
                     TYELFKQIAERGYKVIFTTDHGTIKVKDPVKVIGDKNTNVNLRYKVGKNLSYNKKDVF
                     EITQPAKAGLPSPNVSSTYIFACNDKFF"
BASE COUNT         2860 a         1229 c         1456 g         2596 t
ORIGIN      
        1 tgccaaagct caaacaactg ctggctggtt aagtatttta ttcatggctt ttaccctggt
       61 ggtagtttct ttttcctgta cgggaccgat tattggaact ttgttggtgg aagtttcaag
      121 caatcaatcc attttagctc caaccattgg gatgtttggc tttgctttgg ctctggctgt
      181 tccttttaca ttatttgctt tctttccttc ctggttaaaa tctttgccac ggtcgggtgg
      241 ttggttaaat caggtaaaag tggtgctggc ttttattgaa ttggcttttg cattgaaatt
      301 cctttcagtt gctgatctgg cttatggctg gcacatgctg gatcgggaag tatttttatc
      361 gctctggatt gttattttta ccttattggg aatttattta ttgggaaaaa ttcgttttcc
      421 acacgatagc gaaataaagc atgtttctgt gttagcagtt ttttgggcaa ttatttcatt
      481 ttcgtttgca atctatatga taccaggttt gtggggcgca ccgttgaaag ccatcagtgc
      541 ttttacacca ccgctttata ctcaggattt taatttatat cagcaaaaca tttatcccga
      601 ttttaccgat tatgatgagg gaatgacttt tgctaaagaa aaaaatttac ctgttattgt
      661 tgatttttct ggatatggat gtgtgaattg cagaaaaatg gaagcaaatg tcttgtccga
      721 tcctgaaatc agcaattttt tgaaaaaaca atttgtgcta attactttat atgttgatga
      781 caaaacacct cttcaaaaac cttatgaagt catagagaat ggcaaagtaa gaaaaatcaa
      841 aacagtaggt gacaaatgga gttatctgca acgatataaa tttggtgcta acgctcagcc
      901 tttttttgtg atgctcaaca atgatggtaa acctctcaac aagcctttta ctttcgatga
      961 tcatcctcag cattttttaa aatggttgac aaaaaaatag aacaaaatgt ctttttgttt
     1021 gtttaaaaca agaaaattac tgttattttg tcatttccct attcgtattt aatggcttga
     1081 aaaacaaaaa acaacaattg gcaaagttat tgctaaaata aaaaagatgc ttatatcaat
     1141 tcagcaagaa atttatgctg aagttgagga ataaaaattt taaaaaatga ataaatatca
     1201 aaaagaaaaa atggaaaaag gaaaagatat ggctaataat gaaataaatg aacaagaaaa
     1261 aatttcaggt aagaaagaaa atttagttcc tcaagctgaa aaaacagaag aaaatgaaaa
     1321 tatagaaaag gaactggaga gattaagaat agaatgcgaa gagctggcag ataaaaattt
     1381 gcgtttgata gccgagttcg ataattatcg gaaacgaatg ctgaaagaaa aggcggaatt
     1441 attgaaaaat gcttcagaaa gtgtgctggt aaatctttta ccattggtag atgattttga
     1501 acgtgccttg caagctatcg aaaccactga tgatattaat gctataaaag aaggcgttca
     1561 attaatttat aataaattta ttaattttct acaccagtat aatgtaaaag aaatacctac
     1621 tattcatcaa gaatttgata ctcagttaca cgaagctgtt tcaacaattc cagctcccaa
     1681 tgaagattta aaagggaaaa ttgttgactg tgtattaaaa ggttatactt tgaatgacaa
     1741 agttattcgt catgcaaaag tcattgttgg tgaataaata attttttaga tgttactcaa
     1801 acattcaata aaaatgtctg aggaaagata aaatattatg gcaaaaagag attattatga
     1861 gattttagga atttcacgaa cggctactga agtggaaatt aaaaaagctt atcgtcagaa
     1921 agctatccag tatcatcctg acaaaaatcc gggtgataaa caagccgaag aaaattttaa
     1981 agaagctgct gaagcttacg aagtgttaag tgatcctgaa aaaagagatc gctatgaccg
     2041 atatggacat agcggagtaa gcggttcggg gggaggtggt tttgggcaag gaatgactat
     2101 tgaagatatt ttttctcatt ttggagatat ttttggcggt cattttggtg gctttagtgg
     2161 ctttggtggc ttcagtggat ttagaagtga ttcccaatct ccgaacacaa agatacgtca
     2221 tggttccgat ttacgaataa aagtaaaatt gacgcttgaa gaaattgcca gtggcgttga
     2281 aaaaaaagtg aaggttaaga aatatgttgc ctgtccttcg tgtaagggaa caggtgctgc
     2341 cgaaggttct tctcctgtaa cttgttcaac gtgtagagga actggacgtg taaccagagt
     2401 acaacaaacc attttaggcc gaatgcagac caccgttgag tgccatacct gtaaaggcga
     2461 aggaaaaatt attgttaata aatgtcctta ctgcaacgga gaaggcatta ttcaagatga
     2521 agaaattatt accattaata ttccagcagg agtaagtgaa ggtatgcaac ttacttttgc
     2581 aggtaaagga aatgcagcaa gaagaggcgg tatcaatgga gatctaattg tgttaattga
     2641 agaagaacct catccttctt taatacgtga tgggaatgat ttgatctata atttgttatt
     2701 gagtgttccg caagcagctt tgggtggttc tatcgaaatt ccttctctta caggaaaagt
     2761 aaaagtgaat attgaaccag gaacacagcc aggaaaagtt ttacgtttga aaggcaaagg
     2821 tttacctgat gtaaatcgtt atggcaatgg ggatttattg atcaatattg gcgtttatat
     2881 tccagaaaaa ttaagcaaag aagaaaaaaa tcttctggaa aaattagata aatcaaataa
     2941 ttttaaacca aatcctgcag cttcaaaaaa atttttcagc catttcagaa atatgtttga
     3001 ataacaacgt gttgtgaaat atagtaaaat gaggaaaaca aaattttctt caataaatgg
     3061 aagaataaat tataaatttt ccaggagtaa aaaattttaa ggttaatcaa taaaggtaaa
     3121 gtattatgtt tgtataaaag ttcctccttc tctcgccacc tgcacctcgt aacttaacaa
     3181 acattgcata cgacataaaa actaaaagtc tccactggaa ctccaaacta ttgaccacca
     3241 atgcttaagt agatggtaaa ttgtgatgat tgagtttact gcgattgcaa atactcgcaa
     3301 gagaagtttt tttcctcaag ttgtgaaaaa atttcgtctc gtaattcaag tggtaattcg
     3361 atatttctta attgtaaact atttagccaa tcctttacct cgtttttttg taaagtgtga
     3421 aaaacttctg aaatttgttt aatttgttct tgggtatatt cattggcagg aattcctttc
     3481 aaagcatcta attcctcgtc atcataataa ataatttcat tttccttatt gaaaagttgt
     3541 ttcacctggc attcattgta agcatcgcaa taactacaag ttgctgaagg attgattaaa
     3601 tgattgtttg tcgccttttt cttattaaaa tgagcaaatg caaacaaaat aacgccaagt
     3661 atgattgcaa tgattaaaaa tatccacata ttttctatat taaagtctta aaaagatgtt
     3721 ctaacataat ttatcataca aaattaagca taaatgtgat gatttagcta tttttgtaaa
     3781 ggatataaaa tataaataac atttttaagt tttttaagag gaaatggata ttgtagaaaa
     3841 taataaaatt ccatgtttta ctgattgttt cttttcttaa tttcttaatt tcttaatttc
     3901 ttaatttcat tgaagataaa aaatagaaat acacttttgc ttttactgct gatgctactg
     3961 gttggcagtt gttctcttca gaaaaacacc aagttttctc gtagttatca tgctttgact
     4021 actcgtttta atgtatattt taacgctgaa cagagttatc aggaaggtct aaaaaatatt
     4081 tgggatgcca acaaggacga ttattcaact gttctacccc tttatcccat tagccgacac
     4141 tctaacgctg cggctgctac tgctaacatg gacagaacca ttgaaaaatg ccgtaaagct
     4201 atcaaacttc attctattaa acaaaaaccg gagaaagatt ataaaaagtg gaactctcct
     4261 gaatatcaag ctttctataa tcaagaagaa tttaatccgc ttcttaaaga agcgtggtta
     4321 ttgttaggaa aagcagaatt tcataaaggt gattttttag gttccatagg tactttttcg
     4381 tacatcgctc atcattttgc tgctgatcct gatgttatta ttcaatgtca actatggatg
     4441 gcgagagctt atgcagaaat gggatggctt tatgaagcag aagatgtttt atcaaaaatt
     4501 aatcaagatc aattaaaaaa agagcatgtc gggttatttg ctgctgtaaa cgccgatatt
     4561 ttattaaaaa agaaagaata caaagaagca attccctatt tacaattggc aatagaaaaa
     4621 gaatcagata aaaaattaaa acaacgattc actttcattt tgggacaact ctatcaaatc
     4681 acaggaaatg agaatgcagc atatcaggct tttacacagg tgttaaaatc aaatcctccc
     4741 tttgaaatgg atttttatgc tcgtattcaa cgagctcaat tgaatgcttc caatttctcc
     4801 ctaacacaga aagaactaac caaaatgcta aaaaattcaa aatacaaaga ttatcaagac
     4861 cagatttatt ttgcaatggg taaaaatctg ctgatgaaag gtgatacctt aaaagcaata
     4921 gaaaatttca aatctgccat tgataaaagt acccgtaata gttatgataa agccaatgca
     4981 ctaattacct taggagattt atattacaaa aataaaaatt acaaacaagc ttatccttgc
     5041 tatgaagaag catcaaaaat tattaccaat gaacatgaag attatcagcg agtgtcacgc
     5101 ttatctgaaa tactgagtga atgggtagtt caatatgata ttgtgatgct ccaggatagt
     5161 ttgcagcgat tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntatcagaag
     5281 cttatttcca attgtatctg atggaaagca aaatgggtaa ccgagataaa gctgaacaat
     5341 ggcgaacaaa attaattact gaatttcctg aaacaacttt tgccagatta ctttccaaac
     5401 ctgattatcc tgaacgatta aaacgaatgt atcaagagca ggattctatt tatcgtcttg
     5461 cttatcaagc ttatacaaag aatgatttta atgccgtatt tcagtataaa gaatatatgg
     5521 aacaaaatta tcccacttca ccccttatgc caaaattttt gttccttgaa gctttaagta
     5581 tcggtaaaac atcttcttct gaacgttttg agaaagaatt aagcaattta atcagtgctt
     5641 atcctgaaag cgacgttagc tcgatggcaa aagatatttt agccttgata aaacaaggaa
     5701 aacaagtgca aacaagtagt acgcatggtt ctcttttagc taaacgggaa gaaataatta
     5761 agacagaaaa agaaacagtg gttcaacaac agttttcgcc tgataaaaaa accaaacatt
     5821 tgctgttgct gataagcgat gcagataaaa gagatttgaa tcaattgatg tatcaacttg
     5881 cttcgttcaa tttttctaga tttttaatta aagattttga cattggcatt cagccactgg
     5941 atagcacaag aaatatttta tttgttgcca atttcgactc ttatgatgaa gtcttgtggt
     6001 atttgaatac actttcgtct gatgaattgt tacaatcact tatgaatcaa ttgaatatac
     6061 aaaaggttat tatctctgaa aataattttg ctttattgaa aacaactttc agtttaaacg
     6121 attatttggc tttccacgat aaatttttaa cacccgacaa taaagcaata aaagaaatta
     6181 ctgcgtcttt tgagaaaaaa acagcttcga cagtaatccc ttcttctgct gctgggaaac
     6241 cacaaatagg aggtgaaact caatcgccag caaaagcatt gaaaaatgaa aatgttttaa
     6301 ctcaatcggt tgcaccaact ctaccactgg aaaaacaaac agaagcagct cttccttcga
     6361 caacacctcc aataactaaa gaagaagcgc ctctttacaa aggtttattt gcttataaac
     6421 caaatgagct tcattttgtt actatttatg ttgtttcagg aaatgtggat gagacaaaag
     6481 ttcaaaatgc ttttgataaa tataatcaac aaaactatgg gattttgaat ttgtcaactc
     6541 gagctgagaa agttgggaaa cagcaatttt tcatcattgg accttttcct gatgccaaca
     6601 ctgcaaaatc gtatttgatg cggatagtca aagaatcttc gctttttgaa ggattaaaag
     6661 gggcaaacta tcgtaattta gtaggaacgc aggaaaattt aaatactatg cttcagcaaa
     6721 atgcattgaa tgtgtatttt gattttatga aagaatatta tttaaaatga tttttatgct
     6781 tttcgatgaa caaaatttta ttgggttatg aaacaaggca aaatactctg gatagacgat
     6841 gaaattgatt tgttgcaacc ttatcttatt tttcttaatg acaaagggta tgaggtttta
     6901 actgctacca atggcaatga tgccattgaa atttgtcagc aagaaaattt cgatattatt
     6961 tttctggacg aaaatatgcc tggtttgact gggctcgata cgctggctgt aattaaagaa
     7021 ttattacctg atgttccggt agtgatgatt acaaaaaatg aagaggaaaa cattatgaac
     7081 atggctatcg ggaacaaaat tgccgattat cttacaaaac cagtgaatcc cagtcagatt
     7141 ttattaactt tgaaaaaaaa tattcatata aaagaaatag taaccgaaca tgctacttcc
     7201 acttatcgtt cagaattctc tcaaattgga atgaaaatca acgattcttt aacttttgaa
     7261 gaatggattg atatttataa gaaattggtt tactgggaac tggaactatc cgaaacggat
     7321 agtgggatgg acgaaatgtt acaaatgcaa aaaacagagg ccaatacagc ttttgcaaaa
     7381 tttatcaaaa ccaattatct taaatggttc gaaaatcctg aaactcgccc cttgataagt
     7441 cctgatttat tcaaaacaaa aatttttcct ttacttgata aaggtgaaaa aatttttttt
     7501 gtggtggttg ataactttcg ctatgatcaa tggaaaatta ttcgagatct tttgggcgat
     7561 ttttatcaat tcgaagaaga agagctttat tgtagtattt tgccaactgc tactcaatat
     7621 gctcgaaatg ccattttttc agggttgatg cctcttcaaa ttgaacaaat gttccccgat
     7681 ttatgggtag aagaaattga cgaagaagga aaaaatctga aagaaaagga tttgttggcg
     7741 actcaaatac aacgttttag aaaaaattat gaattttctt atcacaaaat caacgattcg
     7801 aattcttgtc agcgtttaat tgatcaatta ccacgtctgg aaaattgtca gttaaatgtt
     7861 tgtgtcttga actttattga tatgctctcc catgctcgaa cggattcaaa aatggtgaga
     7921 gaattggcta atgatgaaaa agcttatcgg tctcttacgc tttcgtggtt taaatattca
     7981 actacttatg aattattcaa acaaattgca gaacgaggtt ataaggtaat tttcactact
     8041 gatcatggaa ctattaaagt aaaagatcct gtaaaagtga ttggagataa aaacaccaat
     8101 gtaaatttaa gatataaagt aggaaagaat ttgagttata ataaaaaaga cgtttttgaa
     8161 attactcagc ctgctaaggc aggtttaccc agtcctaatg tgagctctac ttatattttt
     8221 gcttgtaacg acaaattttt t
//
LOCUS       DAOTKS010000017         7872 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_9107_length_7872_cov_3.941410, whole genome shotgun sequence.
ACCESSION   DAOTKS010000017 DAOTKS010000000
VERSION     DAOTKS010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7872
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9107_length_7872_cov_3.941410"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="P5236_00755"
     CDS             complement(<1..329)
                     /locus_tag="P5236_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HRU62928.1"
                     /translation="MNKTQQEELFYGCQSTIFGDCCLVFTRQNILRLFFTDEREKTLQ
                     YFEQLTEKMNLKRYDEKAKELIDQIFIERKTFPLVLKGTAFQLSVWKALQTIPFGETT
                     TYKVIAEA"
     gene            complement(338..1408)
                     /gene="pdxA"
                     /locus_tag="P5236_00760"
     CDS             complement(338..1408)
                     /gene="pdxA"
                     /locus_tag="P5236_00760"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005847333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRU62929.1"
                     /translation="MDKIIIGITQGDINGIGYEVILKSLSDAAIFDLFTPVIYGSSKI
                     AAYHRKVLNIQNLSFNNINSVEEANPKRINIINCTSDEVKVELGQPTAESGKAARDAL
                     ERACSDLGKGLIHALVTAPINKKNIQSEKFHFPGHTEYLAEKFGTNKAPLMMLINDFM
                     RIAVVTGHIPLQKVASTLTTELILEKIETLNDSLKEDFLIVRPRIAVLGLNPHAGDEG
                     VIGNEEEQVIIPALKAASEKGIICLGPYPADGFFGSGNFSNFDGVLAMYHDQGLIPFK
                     TLSVESGVNFTAGLSIVRTSPAHGTAYDIAGKNLASENSFREALYMACDIYKNRTWNN
                     EIKSNPLKTIPKSENETNIQVE"
     gene            complement(1434..2462)
                     /locus_tag="P5236_00765"
     CDS             complement(1434..2462)
                     /locus_tag="P5236_00765"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HRU62930.1"
                     /translation="MKNKILILLSAIFFLLGCQKTKTSLPVATGTMFEVIVVMDEPAW
                     SSLPGNTLRETLDQPMEALPQPEPIIDMHHCQHQEFSNMYKSSRNILITEISEKHVQP
                     KVIYTKDKWASPQAIVQITAPNDSSFANTVRQYGNKILDYYVITERERFIEFNKNHLN
                     VKAKTEIEQMFGIQIDVPSFLTKSTKKKDFYWLTNDNPKIRQDLVIYSYPYTDKNTFT
                     KEYLLAKRDTVMKTNIPGEIQGSYMGTEYKNYPPIFREIWVNGGYCAEIKGLWKMING
                     AAMGGPFYSHTRLDEINQKVITVEGFVFAPGEKKRNAIRQLEAVIYTTKLPQEINALK
                     EVSVTPKK"
     gene            complement(2760..5840)
                     /locus_tag="P5236_00770"
     CDS             complement(2760..5840)
                     /locus_tag="P5236_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRU62931.1"
                     /translation="MKKSINIFFKKNWLIFFLLFFGVNICNIQAQQFLTVISDSLTAI
                     AKEETYVKKITISNFSVTQKPKKVIITADERLSYIPFRPENVERIYNALEKILMPTYE
                     GYKIVCQVDNKSIEELIPNFYRTGKKDKSRIFITKKIEFPLVKNLSRPYEITRGLQNK
                     HIAVWPSHGYYYNQNLARWQWQRGRLFQTVEDIYTLSYIYPYLVPMLENAGANVLIPR
                     ERDVQKNEIIVDNDMSDDLSKYSEINQSFQWKNGQDTGFANQKNNYLDGENPFMMGTY
                     RQILSVNNKDKVSSVEWIPFIPQSGKYAVYVSYKTLTKSTSTAHYTVYHKGGQTEFLV
                     NQKIGGGTWIYLGTFYFNEGCNEDSKVVLTNLSSKKNEIITADAVKFGGGIGNIARQP
                     LAEGLITNLKSSEELIIEKNKISDKNFHQDTIHEPEISGYPRFTEGSRYWLQWAGFPD
                     SVYSFTKRKNDYIDDYQSRGLWVNYLIGGSPLAPSEKGLGIPIDLSLAFHTDAGTTSN
                     DSIIGTLGIYTVLNSYQKDIFKNKISRWTSRDLTDIIQTQIVTDIRNMYAPEWTRRRI
                     WNKNYSESRLPEVPSMLLELLSHQNFADMRYGLDPRFQFLVSRSIYKGILKYLHSDNP
                     NKYCVQPLPVKQFSVQFIDTTTLKLRWTNEIDFLEPTAVPEYFVVYIRRDENDFDNGI
                     LVKNNEYTVNIEPGKIYSFKVTAVNKGGESFPSEILSAYRAPDEKGEVLIVNGFTRLS
                     APESFSIDTLYAGFVNDKDAGVPYLYQYNFTGKQYEFSKNKLWVNDDAPGFGASYANY
                     EGKILVGNTFDYPFLHGKAIKEAGYSFSSCSSDAIINEEINLNDYKMVDLILGKQKQT
                     YIGNGEKSSAFKTFPLDLQRKINLFCQNGGNLFVSGANIVSDLCDNPLTLPEERSFLE
                     NTLKLKYCTHQAAVTGEVKFINSPLPFFKKINFSYFHEPNEFCYFVESPDAFEPNDKK
                     AYTICRYSENNLSAAVAYDGNYKVCAFGFPFETIISENDRQNIMESILSFFTKH"
     gene            complement(5847..6887)
                     /gene="rlmN"
                     /locus_tag="P5236_00775"
     CDS             complement(5847..6887)
                     /gene="rlmN"
                     /locus_tag="P5236_00775"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRU62932.1"
                     /translation="MNKKLLIGLTLEELKTIVTDFGMPSYTAKQIAEWLYKKRVLSIN
                     EMTNISSKNRALLNEKYKVGRSEPLQVTISKDGTKKYLFATENNHFIETVLIPEENRV
                     TLCVSSQAGCKMNCLFCMTGKQGFVSKLSAGEILNQILSVPEASEITNLVFMGMGEPF
                     DNTMAVLTALEILTADYGYAWSPHRITVSTIGLIPGMKLFLEKTNCHLAISLHSPFHE
                     ERLQLIPAEKAYPIKKILEELKKYDFSKQRRLSFEYIMFDHFNDTMRHARELVKLLKE
                     IPCRVNLIRFHAIPDVPLKTSSEEKILSFRNYLNENGIIATIRKSRGEDIFAACGMLS
                     TSAKEKETKNQL"
     gene            complement(7642..>7872)
                     /locus_tag="P5236_00780"
     CDS             complement(7642..>7872)
                     /locus_tag="P5236_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HRU62933.1"
                     /translation="RDRKLLLTKRELLKLERANKESGTTIVPLKLFINEKGLAKLEIV
                     LVKGKKLYDKRQSLKEKEDKRNIERAFKQKEH"
BASE COUNT         2519 a         1298 c         1164 g         2891 t
ORIGIN      
        1 gcctcagcaa taactttgta agtggttgtt tcgccaaaag gtatggtttg taaagctttc
       61 cagacagaca attggaaagc tgttcctttt aatactaatg gaaaagtttt tctttctata
      121 aaaatttgat caattagttc ttttgctttt tcatcatacc tttttaaatt cattttttca
      181 gttaattgct caaaatattg aagtgttttt tctctctcgt cggtgaagaa aagtcgcaaa
      241 atgttttgtc tagtaaaaac aaggcagcaa tctccaaaaa tggttgattg acagccataa
      301 aaaagctctt cttgttgagt tttattcatc aacatgttta ttcaacctga atgtttgttt
      361 cattttcaga ttttggaatt gtttttaaag gatttgattt tatttcatta ttccatgttc
      421 tgtttttata gatatcacaa gccatgtaca aagcttctct gaaagaattt tccgaagcta
      481 aattttttcc agctatgtca taagctgttc cgtgagctgg tgaagttcga acaattgata
      541 atccagccgt aaaattaaca ccactttcta ctgataaagt tttaaatgga ataagccctt
      601 gatcgtgata cattgctaaa acgccatcaa aatttgaaaa atttcctgaa ccaaaaaaac
      661 catcagcagg ataaggtccc aaacaaataa ttcctttttc gctggctgct ttcaatgctg
      721 gaataattac ttgttcttct tcgttgccaa ttacgccttc atcgcctgca tgaggattga
      781 gtcctagtac agcaatacgt ggacgaacaa ttaaaaaatc ttccttcaac gaatcattga
      841 gcgtttctat cttttcaaga atcaattctg ttgtcaatgt tgaggcaact ttctgcaagg
      901 gaatatgtcc tgttacgaca gctattcgca taaaatcatt gatcagcatc ataagaggtg
      961 ctttgtttgt tccaaatttt tcagccagat attcagtatg acctggaaaa tgaaattttt
     1021 ccgactgaat attcttctta ttgataggag ctgttaccaa agcatggatt aaaccttttc
     1081 ccagatcaga acaagctcgt tccaaagcat cacgtgcagc ttttccactc tcagccgtag
     1141 gttgacctaa ctctactttg acttcatcgg acgtacaatt tataatatta attcgttttg
     1201 gatttgcttc ttctactgaa ttgatattat taaaactcaa gttttgaata tttaaaactt
     1261 ttctatgata tgctgctatt tttgaagagc cgtaaatgac aggggtgaat aaatcaaaaa
     1321 tagcagcatc agataaagat tttaaaatca cttcataacc aattccatta atatctcctt
     1381 gtgtaattcc aataattatt ttatccatta tcttttcatt atattttttc aacttatttt
     1441 tttggtgtaa cagaaacttc ttttaatgca ttaatttcct gtggaagttt ggtagtataa
     1501 ataactgctt ctaattgtcg gatagcattt cgtttctttt ctcctggcgc aaatacgaat
     1561 ccttctactg ttattacttt ttgattaatt tcatctaagc gagtatggct gtaaaaagga
     1621 ccacccatag ctgcaccatt tatcattttc caaagtcctt taatttcagc acaataacct
     1681 ccattaaccc aaatctccct aaaaatcggt ggataatttt tgtattctgt ccccatataa
     1741 gaaccctgaa tttctcctgg tatattggtt ttcattacag tatcacgttt tgctaaaaga
     1801 tattctttgg taaaagtatt tttatcggta tatggataag aataaatcac caaatcttga
     1861 cgaattttag gattatcatt ggtaagccaa taaaaatctt ttttcttggt agatttggtc
     1921 agaaaactag gaacatcaat ctggatgcca aacatttgtt ctatttctgt ttttgctttc
     1981 acattcaaat gatttttatt aaattcaata aaacgttcac gttctgtaat gacgtagtaa
     2041 tctaaaattt tattgccata ttgacgaaca gtatttgcaa aagaactatc atttggagct
     2101 gtaatctgaa caatagcttg aggagaagcc catttgtctt ttgtataaat tacttttggt
     2161 tgtacatgtt tttcagaaat ctcggtaatt aaaatattcc gagacgattt atacatgttt
     2221 gagaattcct gatgttggca atgatgcata tcaataatgg gttcaggttg aggcaaagct
     2281 tccattggct gatctaatgt ttcacgtaaa gtgtttcctg gtaaagaaga ccatgctggt
     2341 tcatccatca ctactataac ttcaaacatg gtacctgtgg ctacgggtaa agaagttttt
     2401 gttttctggc agcccaacaa gaagaaaata gccgatagta atatcaaaat tttatttttc
     2461 atctctaaat aattttatta agttaaacca tatcagtatt ttaaaaaaac agataagaag
     2521 ttaaaaaaat tgtctttcta cttaaaagta tatgtatttt atttgtttat ctatttgaag
     2581 tcggcttcat ttagttaggt tgattattta cttcaactta tttatggcgg cttatttttc
     2641 tttttaattt gtatttatat tctattttaa aaaaaattgc cagatttgtt tattataagt
     2701 cattaaggtt ttttagaatg ctaaggtttc aattgataat ttttgtttgt taattacgat
     2761 taatgttttg tgaaaaatga taaaattgat tccataatat tttgtcgatc gttttcagaa
     2821 ataatagttt cgaatgggaa gccaaaggca caaactttat aatttccatc ataagctact
     2881 gcagcactca aattgttttc tgaatatctg caaatcgtat aagctttctt atcattaggt
     2941 tcaaacgcat ctggcgattc aacaaaataa caaaattcat ttggctcgtg aaaatagcta
     3001 aaattaattt ttttaaaaaa aggaagaggt gaatttataa atttcacttc tcctgttaca
     3061 gcagcttgat gagtgcaata ttttaatttt aaagtatttt caagaaaaga acgttcttca
     3121 ggcaaagtta atggattatc acataaatcg gaaacaatat tagctccact tacaaataaa
     3181 tttcctccat tttggcaaaa taaattgatt tttctttgca aatcaagtgg gaaagtttta
     3241 aaagcagaag atttctcacc attacctata taagtttgtt tttgttttcc caagattaga
     3301 tctaccattt tataatcatt cagattaatt tcttcattga taatagcatc actactgcaa
     3361 gaagaaaatg aataacctgc ttccttaatt gcttttccat gtaaaaaagg ataatcaaat
     3421 gtattcccca ctaatatttt tccttcataa tttgcataac tggcgccaaa accaggagca
     3481 tcgtcattaa cccaaagttt atttttactg aattcatatt gtttccctgt aaaattatat
     3541 tgatacaaat aaggaacacc tgcatccttg tcatttacaa aacctgcata aagcgtatct
     3601 attgaaaagc tttcaggtgc acttaatcgg gtaaagccat ttacaattaa cacttcacct
     3661 ttttcatcag gagcccgata agcagaaaga atttcagaag gaaaactttc gccaccttta
     3721 ttcacggctg ttactttaaa actataaatt ttacctggtt caatattgac agtatactcg
     3781 ttatttttta ctaaaatccc attatcaaaa tcattttcat ctcttcgtat atacactaca
     3841 aaatattccg gtacagcagt tggttccaaa aaatcgattt cattagtcca tcgcaatttt
     3901 aaagtggttg tgtcaatgaa ttgaacactg aattgcttca caggcaaggg ttgtacacaa
     3961 tatttattgg gattatcaga atgtagatat tttaatatac ctttataaat agagcggctc
     4021 actaaaaatt gaaaacgtgg gtcgagtcca taacgcatat ctgcaaaatt ttgatgagaa
     4081 agtaattcca ataacataga agggacttct ggtaatcttg attcactgta atttttattc
     4141 caaatcctac gtcgagtcca ctctggagca tacatattac gaatatcagt cacaatttgc
     4201 gtttgaatga tatctgttaa atcgcgtgaa gtccagcgtg aaattttgtt tttaaaaata
     4261 tctttttgat acgaattaag gacagtgtaa attcctaaag tgcctatgat agaatcattg
     4321 cttgttgttc cagcatcagt atgaaaagcc agcgacagat caattggaat tcctaatccc
     4381 ttctcagaag gagcaagtgg tgagccacca atcaaatagt tgacccacaa tccgcgtgat
     4441 tgataatcgt caatataatc atttttcctt tttgtaaaac tgtaaacact atcaggaaat
     4501 ccagcccatt gcagccaata acgtgaacct tcagtaaatc gtggataacc acttatttca
     4561 ggttcatgaa ttgtatcttg atgaaaattt ttgtctgaaa ttttgttttt ttcaattatt
     4621 agttcttctg aacttttcaa atttgttatt aatccttctg ccaaaggctg acgtgcaata
     4681 tttcctatgc caccaccaaa ttttacagcg tcagcagtta tgatttcatt cttttttgaa
     4741 cttaaattgg taagaactac tttactgtct tcattgcaac cttcgttaaa ataaaatgtt
     4801 cctaaataaa tccatgtacc gcctcctatt ttttgattga ccaaaaattc tgtttgacca
     4861 cctttatgat atacagtgta gtgtgctgta gaagtgcttt tggtgagcgt tttataagaa
     4921 acatatacgg catatttacc tgactgagga ataaaaggta tccattcaac agagctaact
     4981 ttatctttgt tatttacaga taaaatttgc cggtaagtac ccatcataaa tggattttct
     5041 ccatccagat aattattttt ttgatttgca aaacctgtat cttgtccgtt tttccactga
     5101 aaagattgat taatttctga gtattttgat aagtcatcgc tcatatcatt gtccacaata
     5161 atttcatttt tttgtacatc tctttctctg ggaattaaaa cattagcacc agcattttca
     5221 agcatcggta cgagataagg gtaaatatac gaaagagtat aaatatcttc cactgtttgg
     5281 aaaagtcgtc ctcgttgcca ttgccatcgt gctaaatttt gattatagta ataaccatga
     5341 ctgggccata cagcaatgtg tttgttttga agaccccgag taatttcata aggacgagaa
     5401 agatttttaa ccagaggaaa ttctattttt tttgttataa aaattctact tttatctttt
     5461 ttccccgttc tgtaaaaatt gggtattaat tcttcaatag atttattatc cacttgacaa
     5521 acaattttat aaccttcgta agtaggcatc aaaatttttt caagagcgtt gtaaattctt
     5581 tctacatttt caggtctgaa agggatataa cttaatcttt catcagcagt aataattact
     5641 ttttttggtt tctgggttac tgaaaaatta ctgattgtta ttttttttac ataagtttct
     5701 tctttggcaa tagctgtcaa tgaatcagaa atgaccgtta aaaattgttg ggcttgaata
     5761 ttgcagatat ttactccaaa aaacaacaaa aaaaatatca gccaattttt cttaaaaaaa
     5821 atatttattg attttttcat acagttttac aattgatttt tagtttcttt ttctttagct
     5881 gatgtggaca acatgccgca agcggcaaaa atatcttcac ctcgcgattt acgaatagtg
     5941 gcaataattc cattttcatt caggtaattg cgaaacgaaa gtattttttc ttcactcgaa
     6001 gtttttaatg ggacatcagg aatagcatga aaacgaatta aatttactcg gcaaggaatt
     6061 tctttcaata attttactaa ttctcgagca tgtcgcatag tgtcgttgaa atgatcaaac
     6121 ataatatatt caaaagaaag tcttcgctgt ttactgaaat catatttttt caattcctct
     6181 aatatttttt tgatgggata agctttctca gcaggaatta attgaagcct ttcttcatga
     6241 aagggtgaat gtaaactaat ggcaagatga caattggttt tttccagaaa aagtttcatg
     6301 cctggtataa gtccgatagt cgatacagtt attctgtgag gactccaggc atatccataa
     6361 tcggctgtaa gaatttccaa agcggttaat actgccattg tgttatcaaa aggttctccc
     6421 atgcccataa aaaccaaatt ggtgatttca ctggcttcag gaacggataa aatttgatta
     6481 agaatttctc ctgctgacaa tttagaaaca aatccttgtt ttcctgtcat acaaaacaga
     6541 caattcattt tacagcctgc ctgtgaagaa acgcacagag taacacgatt ttcttcaggt
     6601 atcaaaactg tttcgataaa atggttattt tcagtagcaa ataaatattt ttttgttcca
     6661 tctttcgaaa tagttacttg aagaggttca gaacgaccaa ccttatattt ctcattgagc
     6721 aaggctctgt tttttgaaga aatattggtc atttcattga tagaaagaac ccgtttttta
     6781 tataaccatt ctgcaatttg tttagccgta taagaaggca ttccaaaatc ggttacgata
     6841 gttttaagtt cttctaaagt taaccctatc aataattttt tattcatttt tttgatggtt
     6901 tctattataa ttttttcatg aaaaactttc aaattcaatc atttattcgc tttgagaagt
     6961 ttttcccaat ttttatgagt aaattttacc tcgtattgat ataatttggg agaatatccc
     7021 ttgtaaatgt aggaaattgc ccatttttct tctttcttaa aagagaacca tatttcacta
     7081 tctgttaagc aagctaacct catcattcta tatataaata gctttaacaa atgtagagaa
     7141 tattttgtat tttgagaaaa agtaaaaaat aaattagttg agcagaaaaa atgatttttg
     7201 actccgggaa taaaataaaa gttgtttatt aaatttagtt cttgatattc aatgttttat
     7261 cacttccaaa aatcattaga attggttttt tgactactcc aaaattgaca attgtcattt
     7321 aattttgtaa aaagtatttt tattcaattt aatatatttt aatcatgaga caaatcttac
     7381 cttaaaaatt ttatattttt cattcaaaat atttaaatga agcaggaagt agaaaaaata
     7441 atgaataatc ataataaaga caaatcatgc ttattaaggg ttatagtaat aaagtaaaac
     7501 tgtgtcgaga aataattata atttactttt tgttttttta ttacaatttt ttaatattcc
     7561 tttattcttt tgttattcga aacatgattt ttataattat aaagtaaaaa taaagtaaaa
     7621 ataaattctt atgcagcaaa ttcaatgctc tttttgttta aaggctcttt caatattcct
     7681 tttgtcttct ttttctttca gggattgacg tttgtcatac aactttttcc ctttcactaa
     7741 gacaatctcc aacttagcta accctttttc attgataaaa agcttcagcg gtacgatagt
     7801 cgttccactt tccttattgg ctctttccag cttgagcagt tctcgcttcg ttaataataa
     7861 tttacgatca cg
//
LOCUS       DAOTKS010000018         7867 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_9115_length_7867_cov_4.198029, whole genome shotgun sequence.
ACCESSION   DAOTKS010000018 DAOTKS010000000
VERSION     DAOTKS010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7867
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9115_length_7867_cov_4.198029"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..538
                     /locus_tag="P5236_00785"
     CDS             272..538
                     /locus_tag="P5236_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HRU62934.1"
                     /translation="MKSGIHPTNYRPVVFKDVSNGDYFISRSTVNTKETIEIDGVTYP
                     LVKLEISSSSHPYYTGKAKLVDSAGRVDKFMSRYAKTLEKKNKK"
     gene            complement(602..1264)
                     /locus_tag="P5236_00790"
     CDS             complement(602..1264)
                     /locus_tag="P5236_00790"
                     /inference="COORDINATES: protein motif:HMM:NF023074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3256 family protein"
                     /protein_id="HRU62935.1"
                     /translation="MKKTMNNLKKILIISIFFLQTSLYSQKIENLYVHMPDQLDPVLN
                     SKQRHELIEYAKVGQSDTVTTRNGSKAYLLFLDTLNAQIKIKNTSSSTFEMKLLHFAD
                     GQPFVGMIRTVCAPVCHSMIQFCDTTWNKIPLQFNYPKAIDWLNVKNIQDSTIEVAHL
                     QKILENTFITLTFEQGSNRLIATNNSLDFLSKADKKMVLPYVITQPILFQLQEKTWVK
                     EP"
     gene            complement(1245..5618)
                     /locus_tag="P5236_00795"
     CDS             complement(1245..5618)
                     /locus_tag="P5236_00795"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HRU62936.1"
                     /translation="MQNSITQTLVKELSQKLNTKVSVGEVHIKLLNRLVINDFYIEDQ
                     QKDTLLYVNKVNAHFNLWNLFNKKIIIHSAEFQQLFGNLVFDQQGKPNFDFIIQSFKK
                     PKTTQPQKFEFKIQRFELKNSSFRYTSQKNYQPLPKDKFNIHQFQFDHINGEVAVNVA
                     GKDNVNGSIKKFSGIEKSGFQLKNISSNFHYTSQSLQFPFLDIELPNSMIHLDSLQFD
                     YPKTEDLKNFNDQVRLKIPLHSSYVSPSDFSAFVPSFKNINRKFFIDAETSGKLSSMH
                     FKNIQIKYGDSFSFTANVDLNGLPDFNETFIYADINDLHFKRADLQDFIADLTERPVI
                     LPPAFNQIGTIRYKGNISGFFNNLVTYGKLKSNVGEISTDILFRFGQSFKEFSYDGTL
                     KTPGLKLDKILNNPQFGNLIFNINIKGTKKENNDFQGIIEASVPEFQWKSYSYNNMIF
                     NGNYSNSGFNGTFEMVDDNIAAYFNGIIDLTQQSPTFDFHFKLSHADLNALNLVKKYP
                     LALLSFDSNINMVGNPIDQLNGTIEVDNIQFTNQHKTLNVQKIQLTSRQEPDLTSFII
                     SSDFVNGYMNGRYNYNTLPQTFKKLIQNYLPSLSFDYNSSASQKPNYFNVDLQISNIK
                     ELTEVLQIPYTLKENSIIKGSVDERNNTVNLTADFPSFQKGKNEFKNTTLSLQTENQQ
                     LQLTTNTQLKQKNGFMNIYLKALAGENVVSTLFAWQNTDSIKNAGEFQTVTQLRKENN
                     KTFAQLFILPAEVIISNSLWNIHPSTLDFKPDGSIYVHNFLFDNDQDQYIQIDGIASK
                     NQDDALKFKLKGVDFSFIMNDLVKLKALSLGGFFTGEATMYSALYQPFFEAKMFVKDY
                     QMNHVILGDVDLLSYWDKENKQIYSEATIVDKKDTVATIEGAYIPGKDSIDFIYDLHR
                     VSVEMLKPYLGSVVQNVKGLATGKLRMFGPTKKMGFHGDLFMEKAQVTMFFTNTTYFF
                     NDTIHMRRYSTEFKNITIYDEDGNRGTLNGKLIHNGMFGDLTYNVTVTAKNLLSMNTH
                     PEDNPIFYGKIYADGVVHVFGDEKVTNLFVNAVSKPHTECNINIPTTATASESNFVNF
                     VKKETDIEKEKKIETTIKPESTSNTKVNIQIEVTPDAEMRLIVDPRAGDMIEAKGSGN
                     LRFEFDSYSDIKVFGTYTIEKGNYLLTLQNVIRKEFKIVEGSTMNWTGNPYNAQINVR
                     GIYSLTASLRDLLDESQLMSIPRTTVPVNCILLMTGNLMSPTINFDIDLPSSDESVKQ
                     LVKSTINTKEMMARQIVYLLIFNKFYNPYLQRTANTPIGIGTSEAISFLTSTVSAQLN
                     NLLSQMINSNIISIGIDYQQSESSSSNVQAQVLIQPNDRLIINGNLGYRNDNINLSTN
                     TNKFIGDVDIEWLLTENGKFRLKAYNHTIDRYQLRAAKNTQGIGFMYKADFESFEDLF
                     KYYWQKITFKKDKSSNEKNNE"
     gene            5855..6886
                     /gene="tsaD"
                     /locus_tag="P5236_00800"
     CDS             5855..6886
                     /gene="tsaD"
                     /locus_tag="P5236_00800"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRU62937.1"
                     /translation="MDHITILGIESSCDDTSAAVVRDGILLSNVIANQKVHASFGGVV
                     PELASRAHQQNIIPVVHEALKQANINKKELSAIAFTRGPGLLGSLLVGTSFAKGLALA
                     LDIPIIDVNHLQAHVLAHFIKQSENDNTPQPPFPFLCLLVSGGNSQIVLVKAYNKMEI
                     IGQTIDDAAGEAFDKCAKILGLPYPGGPYIDQLAKEGNPHAFKLNKPHIKGYNYSFSG
                     LKTSFLYLVKDKIKENPQFIQENLNDLCASLQNAIIGVLMSKLKKAAQDLDISDIAVS
                     GGVSANSALRQTFYDYAKKYGWRVYIPPFAFTTDNAAMIAITGYFKYLDKDFCSFDEV
                     PFARVSNTF"
     gene            6893..7483
                     /locus_tag="P5236_00805"
     CDS             6893..7483
                     /locus_tag="P5236_00805"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HRU62938.1"
                     /translation="MKKTFSYIKNKLAPYNGIIYFIVILLVCHFVWKFLVKGDETDQF
                     VSFLGIDLTPFFNFMVNHTAHATKAVLNFLGVDIQLYLNYVMKLSNDGFGIRIIWACT
                     GIKQAYIFFCIIAFYRGPWLKKLWYIPAGLVIIYLYNIFRISLTVALVQNHPSWFTFL
                     HEYVLKYLFYVIIFGMWVLWEEKFVVKKSVKIISKN"
BASE COUNT         2619 a         1230 c         1250 g         2768 t
ORIGIN      
        1 taaatcactg ccgacagttc atattggtaa ttgaagggta atcgattgcc aaaagggtgt
       61 ttatcgatat ttaaagaaat tttaaaccgc atacagcctt gtttgaacaa taagttttgc
      121 ttaaaatatc ttgacagaca aatataaaca ttctttttaa ttttttcgtt aaaaattaca
      181 aaaataaaaa tcgggaaaat tatttgataa atggaagaaa aatagtaact ttgcaaattc
      241 aaaatcaatc agcaattaat tgtataaaga tatgaaaagt ggcattcatc ctacaaatta
      301 tcgtcctgtg gtatttaaag acgtgtcaaa tggcgattat tttatctccc gttcaacagt
      361 gaacacaaaa gaaactattg aaattgacgg agttacttat ccattggtaa aattagaaat
      421 ttcaagttct tcgcatcctt attatacagg aaaagctaaa ttagtggaca gtgcagggcg
      481 tgtggataag tttatgagtc gatatgccaa aacactggaa aagaaaaaca aaaaataaag
      541 gtaagttttt tttcatgggt aagagaagtc ttgtaagttt tttacaaggc ttttctgttt
      601 tttatggctc ttttacccat gttttttcct gaagctgaaa taaaatgggt tgagttataa
      661 cataaggaag gaccattttt ttatctgctt tacttaaaaa atcaaggctg ttattagttg
      721 ctattaagcg attgctacct tgttcaaagg tcagcgttat aaaagtattt tcaagaattt
      781 tctgtaaatg agcgacttct attgttgagt cctgaatatt ttttacattg agccaatcta
      841 ttgcttttgg ataattaaac tgcaagggta ttttattcca tgttgtatcg caaaactgaa
      901 tcatggaatg acatacaggg gcacaaacag tgcgtatcat tcccacaaaa ggttgtccat
      961 ctgcaaaatg taaaagtttc atttcaaaag tgctggaaga agtattttta atttttattt
     1021 gtgcattcaa tgtatctaaa aacaacaaat aagctttgct gccatttcga gtggtaacag
     1081 tatcactttg accaactttt gcatattcaa tcaactcatg cctttgtttc gagttcaaaa
     1141 caggatctaa ttggtctggc atgtgaacgt aaagattttc aatcttttga gaataaagtg
     1201 aggtctgtaa aaagaagata ctgataatca gtattttttt taggttattc attgtttttt
     1261 tcatttgatg atttgtcctt tttaaacgtg attttttgcc agtaatattt gaataaatct
     1321 tcaaaacttt cgaaatcggc tttgtacata aaaccaattc cctgagtatt ttttgctgct
     1381 ctcaattgat aacgatcaat agtatgatta taagctttca aacgaaattt cccattttca
     1441 gtcaataacc attcaatgtc cacatcacca ataaatttat ttgtattggt acttaaatta
     1501 atattatcgt ttctgtaacc gagatttcca ttgataatca atctgtcatt aggttgaatc
     1561 agcacttgag cctgaacgtt gctacttgaa ctttctgatt gttgataatc gatcccaatg
     1621 gagatgatat tgctgttgat catttgtgat agtaaattat tcaattgagc actcactgta
     1681 gaagttaaaa atgatattgc ttcgctggtg ccaatgccta tcggtgtatt agctgttcgt
     1741 tgcaagtatg gattataaaa cttattaaaa atcagcaaat aaacaatctg gcgagccatc
     1801 atttctttgg tattgatggt actttttacc aattgtttta cactttcatc gcttgatggc
     1861 aaatcaatat caaaattgat agttggcgac ataagatttc ctgtcataag cagaatgcaa
     1921 ttgacaggca ccgttgttct tggaatagac attaattgcg attcatctaa caaatcgcgt
     1981 aaagaggctg tcaatgaata aatgcctcgt acatttattt gagcattgta cggattacct
     2041 gtccagttca tagtgctgcc ttctactatt ttaaattctt tgcgaatgac attttgtaag
     2101 gttaacaaat aatttccttt ttcgatggta taagtgccaa atacctttat atcagaataa
     2161 ctatcaaatt caaatcttaa atttccgcta cctttggctt caatcatatc acctgctcgc
     2221 ggatcaacga ttaaacgcat ttctgcatct ggagtaactt caatctggat atttactttt
     2281 gtattactgg tactttcagg ttttatcgtc gtttctatct ttttttcttt ctcaatatct
     2341 gtttcctttt ttacaaaatt aacaaaatta ctttcggaag cagtagcagt ggttggaata
     2401 ttaatattac attctgtgtg tggtttagaa accgcattta caaatagatt ggtaactttt
     2461 tcatcgccaa agacatgtac cactccatct gcataaattt ttccgtaaaa aattggatta
     2521 tcttcaggat gagtattcat cgacaaaaga ttttttgcag ttactgtaac attataggtt
     2581 aaatcgccaa acatgccatt atgtataagt tttccattta aagttccacg attaccgtct
     2641 tcgtcataga tagtaatatt tttaaactcc gtagaatatc gcctcatgtg gatggtatcg
     2701 ttaaaaaaat aagttgtatt agtaaaaaac atggttacct gggctttttc cataaataaa
     2761 tctccgtgaa accccatttt tttcgtagga ccaaacattc gtagttttcc tgtggcaagt
     2821 ccttttacat tttgaactac gcttcccaga taaggtttta acatttcaac agatacccga
     2881 tgcaaatcat aaataaaatc aatagaatct tttcctggta tataagcacc ttcaattgta
     2941 gctacggtat cttttttatc cacaatagtg gcttcagaat aaatttgctt gttttcttta
     3001 tcccaataac ttaataaatc gacatcaccc aatattacat gattcatttg ataatctttt
     3061 acaaacattt ttgcctcaaa aaaaggctga tacaaagcac tatacatggt ggcttctcct
     3121 gtaaaaaaac cacctaaact caaggctttc agtttaacca aatcattcat aataaatgaa
     3181 aaatcaacac ctttgagttt aaatttcaga gcatcgtcct gattttttga agcgatgcca
     3241 tctatctgaa tatattgatc ttgatcatta tcaaaaagaa aattatgaac ataaatagac
     3301 ccatcaggtt taaaatcaag ggttgaagga tgaatattcc atagcgaatt tgaaataatg
     3361 acctcggcag gtaaaataaa caattgagca aatgttttat tattctcttt tcggagttga
     3421 gtaacggttt gaaattcacc agcattttta atagaatctg tattctgcca ggcgaataag
     3481 gtacttacaa cattttctcc tgccaaagct tttagatata tattcataaa accatttttc
     3541 tgcttcagtt gagtgtttgt agtcaactgt aattgttggt tctcagtttg aagcgaaagg
     3601 gtagtatttt taaattcgtt tttccctttt tgaaaggatg ggaaatcagc agttaaatta
     3661 acagtgttat ttctctcatc cacagaacct ttgataatcg aattttcttt taaggtataa
     3721 ggaatttgta aaacctccgt gagctctttg atatttgaaa tttgtaaatc tacattgaaa
     3781 taattgggct tctgggaggc cgacgaatta taatcaaaag ataaagaagg taaataattc
     3841 tgtatcaatt tcttaaaagt ttgaggaagt gtattgtaat tgtatcttcc attcatataa
     3901 ccattcacaa aatcagaaga aataataaaa cttgtaagat ccggttcttg acgagaagtt
     3961 aattgaattt tttggacatt caaagtttta tgttgattgg tgaattgaat attatcgact
     4021 tcaatagtac cattaagttg atctataggg ttacctacca tattgatgtt actgtcgaaa
     4081 gataataaag caagaggata tttttttacc agatttaagg catttagatc tgcatgggag
     4141 agtttaaaat gaaaatcaaa agtaggggat tgctgagtta aatctattat accattaaaa
     4201 taagctgcaa tattatcatc aaccatttca aaagtgccat taaaacctga attactgtaa
     4261 tttccattga aaatcatatt gttataagaa tagctcttcc attgaaattc tgggacagaa
     4321 gcctcaataa ttccttgaaa atcattgttt tcttttttag ttcctttaat attaatatta
     4381 aaaattaaat taccaaattg tggattgttt aaaattttat ctaatttcaa acctggagtt
     4441 ttgagagtgc catcatagga aaattctttg aaactttgac caaatcgaaa taaaatatct
     4501 gtagaaattt ctccaacatt cgactttaat tttccgtaag taaccagatt attaaaaaat
     4561 ccagaaatat ttcctttata acgaatggtg ccaatctgat taaaagcagg tggtaaaata
     4621 acgggacgtt ctgttaaatc agcaataaaa tcctgtaaat cagctctttt aaaatgtaaa
     4681 tcgttaatgt cagcataaat aaaagtttca ttaaaatctg gaagtccatt taaatcaaca
     4741 ttggctgtaa acgaaaatga atctccatat ttaatctgaa tatttttaaa atgcatgctg
     4801 gaaagttttc cacttgtttc tgcatcgata aaaaactttc tattgatatt tttaaaagaa
     4861 ggtacaaaag cagaaaaatc agaagggctc acatacgaag aatgaagcgg aatttttaag
     4921 cgaacctgat cgttaaaatt tttcaaatcc tctgtttttg gataatcaaa ctgcaagctg
     4981 tccagatgaa tcatagaatt tggaagctca atgtcaagaa aaggaaattg caatgattgg
     5041 ctagtataat gaaaatttga agatatgttt tttaattgaa agccagactt ttcaatacca
     5101 ctgaatttct ttatcgaacc attgacatta tctttacctg caacatttac tgcaacttca
     5161 ccattgatat ggtcaaactg aaactgatga atattaaact tatcttttgg caaaggttga
     5221 taattttttt ggctggtata acgaaaggaa gaatttttta gttcaaaacg ctgtatctta
     5281 aattcaaatt tttggggctg agtagttttt ggctttttaa aagattgaat aataaaatca
     5341 aaattgggtt ttccttgctg atcgaaaacg aggttgccaa ataattgttg aaattcagca
     5401 gaatgaataa tgattttttt attaaataaa ttccataaat taaaatgggc gttgacttta
     5461 tttacataca aaagagtatc tttttgctga tcttcgatat aaaaatcatt gataacaaga
     5521 cgattaagca attttatatg aacttcacca actgaaactt tcgtatttaa tttttgagac
     5581 aattctttaa caagcgtttg ggtaatagaa ttttgcacct tgctgttttg caacaaggta
     5641 aataccaatg ctaagaaaat aaggatgcca gcaatggtat agcttattat ttttattgtt
     5701 ttttttatac ctttgagatt ttatttacaa aagtaaaaaa attatttcct atttccagta
     5761 atctaagctg gcaaaatagt agcttttcca aatatttaat tgcttttgca gtttttatat
     5821 gagattgctt tttttgtaaa aaattgttag agaaatggat catatcacta tattaggcat
     5881 tgaatcttcg tgcgatgata cttcagccgc tgttgtacgc gatggtatat tgctgtcgaa
     5941 cgtaattgct aatcaaaaag tacatgccag ttttggtggc gtagttccag aacttgcttc
     6001 tagggctcat caacaaaaca ttattcctgt cgtacacgaa gcattaaaac aagccaatat
     6061 taataaaaaa gaactgagtg ccattgcttt tacgcgtgga cctggtctgt tagggtcgct
     6121 tctggtgggc acttcatttg ccaaagggct tgcgctggct cttgatatcc ctatcattga
     6181 tgttaaccat ttacaagcac atgtgttggc acattttatc aaacaaagtg aaaatgataa
     6241 tacaccacaa cctcctttcc catttttatg tttgctggtg tcgggtggca attcgcagat
     6301 tgttcttgta aaagcttaca acaaaatgga aattatcgga caaacaattg acgatgctgc
     6361 cggagaagct ttcgacaaat gtgcaaaaat attggggtta ccttatcctg gtggtccata
     6421 tatcgatcaa cttgccaaag aagggaatcc acatgctttt aagttaaata agcctcatat
     6481 taaaggttat aattatagtt ttagtgggtt aaaaacttct tttttgtatt tggtaaaaga
     6541 taaaataaag gaaaatcctc agtttattca agaaaatctt aatgatttat gtgcttcact
     6601 gcaaaacgct atcattggag tactcatgtc aaaactaaag aaagctgcac aagatttaga
     6661 tatttcagat atagcagttt caggcggcgt atcagctaac tcggctttgc gacaaacatt
     6721 ttacgattat gcaaaaaaat atggctggag agtttatatc cctccttttg cttttactac
     6781 cgataatgct gcaatgattg ctataacggg ctatttcaaa tatctcgaca aggatttttg
     6841 ttcattcgat gaagttccat ttgcacgagt ttccaatact ttctgatttt caatgaaaaa
     6901 aactttttcg tatattaaga ataaattagc tccttataat ggcatcattt atttcatagt
     6961 aattttactt gtttgccatt ttgtatggaa atttttagta aaaggtgatg aaactgacca
     7021 atttgtctct tttttaggta tagaccttac tccctttttc aactttatgg ttaaccacac
     7081 agctcatgca actaaagctg ttcttaattt tttgggagtt gacattcaac tctatctaaa
     7141 ctatgtgatg aaactaagta acgatggatt tggtatcaga atcatctggg catgtaccgg
     7201 aataaaacaa gcttacattt ttttctgcat cattgccttt tatcgaggtc cgtggttaaa
     7261 aaaactctgg tacatacctg ctggacttgt tatcatatat ctttataata tttttagaat
     7321 cagtttaacc gttgcactag ttcaaaacca tccttcgtgg tttacttttt tacacgaata
     7381 tgtattaaaa tatctgtttt atgtcattat atttggcatg tgggtattgt gggaagaaaa
     7441 atttgttgtt aaaaaatcgg tcaaaatcat ttctaaaaac taatccagca actttattat
     7501 caataaaaaa ttgataataa gtaaaaataa tcagtttttt aatcacattt gtgaatagct
     7561 aaattgaatt cattcctaag taagaaattt catgttatac actaattcat caatttatac
     7621 gtaatagaag aaaaaaaccg ctataatgtt tatatagagt tttttataat ttcgcaatta
     7681 cgtatttacg tgattaacta taaaattctt tccaacaaaa tatcctcttt gagcatgata
     7741 ccaaattgca attataaata caatttagag tttaaattaa tgacaaattt ggacctataa
     7801 aaatttccat cgaaatccta cattaaaagt aaatcctggt tgagcaattc caccataatc
     7861 ttcataa
//
LOCUS       DAOTKS010000019         7519 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_9552_length_7519_cov_4.422294, whole genome shotgun sequence.
ACCESSION   DAOTKS010000019 DAOTKS010000000
VERSION     DAOTKS010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7519
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9552_length_7519_cov_4.422294"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            634..3444
                     /gene="uvrA"
                     /locus_tag="P5236_00810"
     CDS             634..3444
                     /gene="uvrA"
                     /locus_tag="P5236_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRU62939.1"
                     /translation="MSKAGNIFVKGARVHNLKNIDAVIPRNKLVVITGLSGSGKSSFA
                     FDTLYAEGQRRYVESLSAYARQFLGRMSKPEVDFISGIPPAIAIEQKVNTRNSRSTVG
                     TSTEIYEYLKLLYARIGKTYSPISGTLVKKHEAEDVVETALSYPDGTTITIMIPIHLN
                     GDRNLKEQLQSFQIQGFSRVEIKGETWRIQDVLKNNLLIDTSEDIYLLVDRVTIDHTS
                     ATISRLTDSIETAFYEGDGICLLKVATEQKEKILSFSKKFEADGIEFEVPTEHMFSFN
                     NPLGACPVCEGFGKIIGIDEDLVVPNKSLSVYEDAIACWRGEVMSEWKKRLIATAHKF
                     DFPIHKPYNELTESQRQLLWTGNEYFPGINTFFKFLEENQYKIQYRVMLARYRGKTVC
                     PSCKGTRLKKEANYVKIGGKAITELILLSMEDLYQFFEKLQLDEYDQMIANRLLTEIK
                     NRIQFLLDVGLGYLNLNRLSNSLSGGESQRINLATSLGSSLVGSMYILDEPSIGLHPR
                     DTEKLIHVLKELRDLGNTVIVVEHDEEIIRAADYLIDLGPGAGRLGGEIVYTGEANVK
                     NDSSIENKIQNSWTLKYLIGTDEISIPAHRRKWNNYIEVIGASENNLKNLNVKFPLNV
                     MTVVTGVSGSGKSSLVEDVLYPALKKYYGGITDKPGKFSDLRGSLQLIHDVELVDQNP
                     LGRSSRSNPVTYIKAYDEIRKLFAEQQLSKQMGYTPSYFSFNTEGGRCEECQGEGVVT
                     VEMQFMADLVMECEQCHGKRFKQDILEVTYQGMNIYDVLEMTVNQAIEFFGQSKGTLE
                     KKIVKRLKPLQEVGIGYVKLGQSSSTLSGGESQRVKLASFLANEKDEPTLFIFDEPTT
                     GLHFHDIKTLLKAFDSLILKGHTILIIEHNMEVIKCADHIIDLGPEGGEKGGYLVFEG
                     TPEELIHCEASYTGKFLKEKLK"
     gene            3561..3660
                     /gene="ffs"
                     /locus_tag="P5236_00815"
     ncRNA           3561..3660
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="P5236_00815"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3702..>4341)
                     /locus_tag="P5236_00820"
     CDS             complement(3702..>4341)
                     /locus_tag="P5236_00820"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HRU62940.1"
                     /translation="VKFVNALIAGILAGTAFQIFQMLYIKGQINLSRYNAVYGGFAAI
                     PLLLLWLRISCLILLLGAEISYAAQSIHDYEFDLDIQNISLRYKKFISLFITYVIIKQ
                     FEQQKPPLSAQQIASEYKLPIRLVHQTLNQLVNMSILIEVFSEKTKSKTYQPALDIHQ
                     VTVGMLYEKIETYGSDLFLSNKNEWLDAFWQKITVLQQKNKGNLSQVLVKDI"
     assembly_gap    4342..4351
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4352..5027)
                     /locus_tag="P5236_00825"
     CDS             complement(<4352..5027)
                     /locus_tag="P5236_00825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HRU62941.1"
                     /translation="MSSLKDFFVRIYNFIRYDIWKVAETELSKGRKIVYRCVKTIIIA
                     TKGYIDDKLGIRASALTYSLFFAVVPIIALVISVGKGFGMEKMIENTLQKTFIAQADV
                     IPTLMSFVKKYLETMQGGVFIGVGIVILLWSVTNFFRLIENTFNNIWQVKKSRSPIRQ
                     FTTYFSIILILPILIILSSGFSIFLNTTFSQISFLNFLSPVLNFGAKLLPYFLNWLIF
                     TLMYLLI"
     gene            complement(5166..5522)
                     /gene="rnpB"
                     /locus_tag="P5236_00830"
     ncRNA           complement(5166..5522)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="P5236_00830"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(6050..>7519)
                     /gene="groL"
                     /locus_tag="P5236_00835"
     CDS             complement(6050..>7519)
                     /gene="groL"
                     /locus_tag="P5236_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617860.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRU62942.1"
                     /translation="SVAKEIELEDPFKNVGAQLLKEVASKTSEDAGDGTTTATVLAQA
                     IVNVGLKNVTAGANPMDLKRGIDKAVAEVVKSIANQSKQVGDDYNKIEQVATVSANND
                     REIGKLIADAMRKVSKEGVITIEEAKGTETTIEMVEGMQFDRGYISAYFVTNAEKMLV
                     EMEKPYILIHDKKISNFKELLPILEPVAQSGRPLLIIAEDVESEALTTLVVNRLRGSL
                     KVCAVKAPGFGDRRKEMLEDIAILTGGVVITEERGLQLEKATMDMLGTAEKITVDKEN
                     TTIVNGAGSKEAIQARIKQIKAQIANATSDYDREKLQERLAKLAGGVAVLYVGAPSEI
                     EMKEKKDRVEDALSATRAAIEEGIIPGGGVAYIRAIEALEKLKGENDDENTGIEIVKR
                     AIEEPLRQIVENAGKEGAVIVNKIKEGKDDFGYNARTENFENFYETGVLDPAKVARIA
                     LENAASIAGMFLTTECVITEKKEPAPVVQQPMPGMGDYE"
BASE COUNT         2509 a         1268 c         1266 g         2466 t
ORIGIN      
        1 cttttccaga aaaatatgtc agccaaaagc taaatttttc tttcaacgca aacacaatct
       61 tttccaaaaa tgaggctttg ccccaaacct cacttactat tctgttttag aaagtgtttt
      121 aattttttaa ttgtttcagt gattctcgct caattatctt aacataaaaa ataaacaaaa
      181 aatttaactt cgttgaagca gctatgtagc tttcaaaact gcatcgttct atatttcaaa
      241 actcgaaact tgtcagtttc cggctcatcg gataaggtca attcaaaaca acatgattaa
      301 ttgataaata taatttgata taaaatttat cagatatcaa aatgcaaaac atttgagtaa
      361 ttttcaatat tttatagact tttcattaac ttcattacca ttatattata atgacaattc
      421 tcagtttaga aacaaaaatt ctacaattgt tgagttaaat gtggataatc attttatttt
      481 attttcaagc acttttttaa ctgcgaaaca tttattagta aaattatttt cagataaaaa
      541 attaattttt tatactcttt tatttataag agtagcttat taatttttga ggaatgttgt
      601 atctttgcag tttaaaaaaa tttctctaaa actatgtcca aagctggaaa tatttttgta
      661 aaaggagctc gtgtacataa tttaaaaaat atagatgccg tcattcctcg gaacaaattg
      721 gtagtcatca ctggtttatc tggttcagga aaatcttctt ttgcttttga tactttatat
      781 gccgaaggac aaagacgtta cgtagaaagt ctttctgctt atgccagaca atttttggga
      841 aggatgagca aaccggaggt agattttatt tcaggtattc caccagccat tgccattgaa
      901 caaaaagtaa atacacgcaa ttctcgttcc actgttggaa cttccacaga aatttatgaa
      961 tacctcaaat tactttacgc tcgaatagga aaaacttatt ctcccatttc tggaacatta
     1021 gtaaaaaaac atgaagctga agatgttgta gaaactgctt tgtcttatcc tgatggcacc
     1081 actattacca ttatgatacc cattcatcta aatggagacc gaaatttaaa agaacaattg
     1141 caaagctttc agatacaagg ctttagtcga gtagaaataa aaggggaaac ttggcgaatt
     1201 caggatgtgt taaaaaacaa tcttttaatt gatacatctg aagatattta tttgcttgtt
     1261 gatcgagtaa ctattgatca tacgtctgct actatcagta gattgacaga ttctattgaa
     1321 acagcctttt atgaagggga tggtatttgc ctattgaagg ttgcaacaga acaaaaagaa
     1381 aaaatattgt ctttttctaa aaaatttgaa gcagatggca ttgagtttga agttccaact
     1441 gaacacatgt tcagtttcaa caacccactt ggggcttgtc ctgtgtgcga aggattcgga
     1501 aaaattatcg gtatcgacga agatttagtt gtacccaata aatctctatc agtttatgaa
     1561 gatgcaattg cttgttggcg aggtgaagta atgagcgaat ggaaaaaaag gctgatagcg
     1621 acggctcata aatttgattt cccaattcat aaaccttata atgaacttac tgaaagtcaa
     1681 cggcaactgc tctggacagg caatgaatat tttccaggta taaatacttt cttcaaattt
     1741 ctggaagaaa atcagtacaa aattcaatat cgtgtcatgc tggctcgtta tcgaggaaaa
     1801 acggtttgtc catcctgcaa aggaactcga ctcaagaaag aagcaaacta tgtaaaaatt
     1861 ggcggaaaag caatcacaga acttattttg ctttctatgg aagatttgta tcaatttttt
     1921 gaaaagcttc aattggatga atacgaccag atgattgcca atcgcctttt aactgaaata
     1981 aaaaatcgta ttcagttttt gctggatgtc ggattgggat atttaaactt gaatcgtttg
     2041 tcaaattcgc tatcaggagg agaaagtcag cgaattaacc tcgcaacttc actgggaagc
     2101 agtttggtgg gttccatgta cattcttgat gaaccaagca ttggcttaca tcctcgtgat
     2161 actgaaaaat taattcatgt tttgaaagaa ttaagagatc ttggcaatac agttattgta
     2221 gtagaacatg atgaagaaat tattcgagca gccgattatc tgatagattt aggtccagga
     2281 gctggcagac ttggtggtga aattgtatat acaggcgaag ctaatgtaaa aaatgattct
     2341 tcaatagaaa acaaaattca aaattcctgg actttaaaat acttaatagg aacagacgaa
     2401 atttccatac cagctcatcg aaggaaatgg aacaattata ttgaagtaat tggtgcaagt
     2461 gaaaataatt taaaaaatct caatgttaaa tttcctctta atgtaatgac agtggtgact
     2521 ggcgtaagtg gttcaggtaa atcatcgtta gtagaggatg tcttatatcc agctttaaaa
     2581 aaatattatg gtggaattac cgacaaaccg ggaaaatttt cagatttacg gggcagtttg
     2641 caattaattc atgatgttga attggtggat caaaatccat taggacgttc atctcgctct
     2701 aatccggtga cttacattaa agcttatgat gaaatcagga aactttttgc tgagcaacaa
     2761 ctttccaaac aaatgggtta caccccttcc tatttttcat tcaatacaga aggcgggcgt
     2821 tgcgaagaat gtcaaggcga aggagtagta actgttgaaa tgcaatttat ggctgatttg
     2881 gtaatggaat gtgagcaatg tcatggaaaa cgcttcaagc aagatattct ggaagtcact
     2941 tatcaaggaa tgaatattta tgacgttttg gaaatgactg tcaaccaggc tattgaattt
     3001 tttgggcaat caaaaggaac gcttgagaaa aagattgtga aacgtttaaa acctttgcag
     3061 gaagtgggaa taggctatgt aaaacttggg cagtcctcta gtacactttc aggtggcgaa
     3121 agtcagcggg tgaaacttgc ttcttttctg gcaaatgaaa aagatgaacc aaccttgttt
     3181 atctttgacg agcctactac aggtcttcat ttccatgata ttaaaacttt actgaaagct
     3241 tttgattcgt tgattttgaa agggcatact attcttatta ttgaacacaa catggaagta
     3301 attaaatgcg ctgatcatat catagattta ggtcctgaag gtggtgaaaa aggaggttac
     3361 cttgtttttg aaggaacgcc agaagaactt attcattgtg aggcttccta tactgggaaa
     3421 tttttaaaag agaaattgaa ataaaccttt tatttttgaa tcgtttccta aattttaaaa
     3481 agtgataaat actttttagc gattttgaaa attcttccta aaaatttggt tagtatctta
     3541 taaaagtata tttttgtatt gggtaagtcc tacacgacca gctcctttca aactccccca
     3601 gggcttgacc gcagcaaggg tagaaagttg tagcggtgcg atgtaggggg cttacctttt
     3661 tttatgtata ttttaatttt cagtttaatt tcaggaaaaa attatatatc ctttacaaga
     3721 acttgagaca gatttccttt atttttttgt tgtaaaacgg taattttttg ccagaaagca
     3781 tccaaccatt cattcttgtt tgacaggaac aaatctgaac cataagtttc aatcttttca
     3841 tatagcattc caacggtaac ttgatgaata tctaaagctg gttgataggt tttagatttt
     3901 gttttttctg aaaaaacttc aatcaatatt gacatattta ctaattgatt taaagtttga
     3961 tgtactaatc gaatgggaag tttatattca gaagcaattt gttgagccga taaaggaggt
     4021 ttctgttgtt caaattgttt tattatcaca taagtgataa aaagagaaat aaatttttta
     4081 tagcgtaaac ttatattttg aatatccaga tcaaattcat aatcatggat actttgagct
     4141 gcataggaaa tttcagcccc aagtagaaga atcaaacaag aaattcgcag ccagagtaaa
     4201 aggagtggaa tggctgcaaa acctccataa acggcattat accgcgatag gttaatttgt
     4261 cctttgatat atagcatctg gaaaatctga aaggctgttc ctgctaaaat acctgctatc
     4321 aatgcattga caaattttac tnnnnnnnnn ngtattaata aatacatcaa tgtaaaaatc
     4381 aaccagttaa gaaaataagg aagtaattta gctccaaaat ttagtacggg acttagaaaa
     4441 tttaaaaaag aaatttgaga aaaagtggta tttaaaaaaa tagaaaaacc gctggaaaga
     4501 ataataagta ttggaagaat aagaataatg gaaaaataag tggtaaattg tcgaattggg
     4561 gaacgggact tttttacttg ccagatattg ttaaaagtat tttctatcag tctaaaaaaa
     4621 tttgtaacag accataataa aattacaatg ccaacaccaa taaatacccc cccttgcatg
     4681 gtttccagat atttttttac aaaagacatc agggtgggaa tcacatcggc ttgagctata
     4741 aaagttttct gaagggtgtt ttcaatcatt ttttccattc caaaaccctt accaacagaa
     4801 ataaccagtg caattatagg aactacagca aaaaaaagag aataagttaa tgccgaagcc
     4861 cgaatgccaa gcttatcatc tatataaccc tttgtggcta taatgatagt ttttacacat
     4921 cgataaacaa ttttacgacc tttacttaat tctgtttcag caactttcca aatgtcgtaa
     4981 cgaataaaat tataaatgcg aacaaaaaaa tctttcaacg aagacatctt atttaatttt
     5041 ttaggagctt agaaataaaa aatatcttca aaaattcgat gaaaattttt tgattaaatt
     5101 taaaatttat acaacatgta atgaagggat tttttgaact ttatcaaaat aaaaaggtaa
     5161 taataaaaaa tagcaggctt gtaagccggg ttctgtactt ttttcaaagc gtttgccatt
     5221 tatctgaaac aaatgtcacc atttgctttt agcgatctac cccccgacat cggacgagcc
     5281 accctacatg cgtcggtata tttgatcttg caacccataa gtcgtacggc tatgattgtc
     5341 accaatcata ccggtgagct cttacctcgc cttttcaccc ttatctcata gaaatgagac
     5401 ggttattttc tgttacgttt ctctaccttt gcagatagct tcctgttagg aagtatggtg
     5461 ctctgtgttg cccggacttt cctcttctcc tttgaaaaag aagcgacaaa ccagcctgct
     5521 attttaatac aaatatacaa aaaaattaaa ttaataaaaa attctttcac tgaatttaac
     5581 tttcaaaata catagaaaaa ctctttggat ttaacatttt atatgacaaa ttctgtgctg
     5641 tttttttaat aagaaaatgg gagaaaggaa tattcacaaa tttatataat attttttttt
     5701 aatatcaagt agctttcaaa aatgaaaaat gaatttagaa aaaagtttaa agttggtgat
     5761 ttacagatca cgaaattgta ggatagtgaa aatattttag ctgataatca atagcaacaa
     5821 gtaaataaaa taaaataaaa tgcaaagtca taaaagcaaa aagattgttg ttgtctgaca
     5881 agccgaaaaa acaagtgtag acgaagtaga gaaaaatctt ctgttacaaa gaatttgtaa
     5941 tcccttacca acaggcagat gaatgttagt tttaaaataa gaacaaatta aaaaaaggct
     6001 tctgagtatt aatttttcca gaagcctttt tttattttaa aaatttttat tattcataat
     6061 cgcccatgcc aggcattggt tgctgtacaa caggggcagg ttcttttttc tcagtgatta
     6121 cacattcagt agttaagaac atgcctgcaa tcgaagcagc attttcaagt gcaatacgtg
     6181 ccacttttgc tggatccaat actccggttt catagaaatt ctcaaagttt tctgtacgag
     6241 cattgtagcc aaaatcatct ttcccttcct tgattttatt tacaattact gcaccttctt
     6301 tcccagcatt ctccacaatt tgacgaagtg gttcttctat tgcacgcttt acaatttcaa
     6361 tacccgtatt ttcgtcatca ttttctcctt taagtttttc aagagcttca atagcacgaa
     6421 tataagcaac tccaccacca ggaataatac cttcttctat tgcagcacgg gtggcactca
     6481 atgcatcttc tactcggtct tttttctctt tcatttctat ttcggaagga gcacccacat
     6541 ataacactgc tacacctccc gctaatttag ctaaacgttc ttgcagtttt tcacgatcat
     6601 aatcagaagt tgcattggca atttgtgctt taatttgttt aatgcgtgct tgaatagctt
     6661 cttttgaacc agctccatta acaattgtcg tattctcttt gtcaactgtt attttttctg
     6721 ctgttcctaa catgtccatt gtagcctttt caagctgaag tcctctttct tcggtgataa
     6781 ctacacctcc cgttaaaata gcaatatctt ccagcatttc ttttctacga tcgccaaaac
     6841 ctggagcctt aacagcacaa actttcaatg aaccacgaag acgattaact accagagtag
     6901 taagagcttc gctttcaaca tcttcagcta taattaacaa aggacgacct gactgtgcaa
     6961 ctggttcaag gataggaagc aattctttga aattggaaat tttcttatca tggattaaaa
     7021 tgtagggctt ttccatttca acaagcattt tttcagcatt agtgacaaaa taagcagaaa
     7081 tgtaaccccg atcgaattgc attccttcga ccatctcgat agttgtttcc gttccctttg
     7141 cctcttctat ggtaataaca ccttcttttg aaaccttacg cattgcatcg gcaattaatt
     7201 taccaatttc acggtcgttg ttagcagaaa cagttgctac ctgttcaatt ttattgtaat
     7261 cgtcacctac ttgttttgat tggttggcta tactttttac tacttcagct actgctttat
     7321 caattccacg ttttaaatcc attggatttg ctccagcagt tacatttttt aaacctacat
     7381 taacaatagc ctgagctaaa actgtggcag tagtggtgcc atctccagca tcttcactgg
     7441 tttttgaagc tacttctttc aataattgag ctccaacatt cttaaaagga tcttccaatt
     7501 ctatttcttt tgctacgga
//
LOCUS       DAOTKS010000020         7362 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_9795_length_7362_cov_4.222389, whole genome shotgun sequence.
ACCESSION   DAOTKS010000020 DAOTKS010000000
VERSION     DAOTKS010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7362
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9795_length_7362_cov_4.222389"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="P5236_00840"
     CDS             complement(<1..279)
                     /locus_tag="P5236_00840"
                     /inference="COORDINATES: protein motif:HMM:NF027479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HRU62943.1"
                     /translation="MNARQFPLLLLVIATVSSAAVEINLKVTNPNKKFVANAPVVIQL
                     QNLKNISSNERSQLAVYVDNKQISSQLDDLNKDGIQDELVFLLDLKGKE"
     gene            817..1440
                     /locus_tag="P5236_00845"
     CDS             817..1440
                     /locus_tag="P5236_00845"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRU62944.1"
                     /translation="MLKIILKGIIIGWFISGPIGPIGMLVIQRTMNRGVKYGIATGVG
                     ATFSDLIYTVITLFCLNFVIGFIEKNQLIIELIGSIIVIFFGYFIFKSNPALQPKPNE
                     PAHDNLFKDFISSFVLTFSNPLIIFVLIALFARLNFITRATNSFLILCGFLSIFAGAM
                     IWWLALTFLVSHFRSKVSIHQLKAINQITGSIIMLIGLVGTVVSFLK"
     gene            complement(1752..2522)
                     /locus_tag="P5236_00850"
     CDS             complement(1752..2522)
                     /locus_tag="P5236_00850"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HRU62945.1"
                     /translation="MQGIVTLSLVPLRVEDNECSEMYSQLLFGERVEILETRERWLKI
                     RNLADNAVGWIDRKMIEILDSIENESIESCYCTLVPIAEVRIINKNEKMLLPGGSLLP
                     DYENGKFHIQNNIFEVNPDEAGRPLKPSGNQLISLAKQYLNSPYLWGGKSALGIDCSG
                     LTQVIFSICGIFLPRNAYQQAEKGKQVPSLKEVHAGDLAFFQNSEGKIVHVGLLLNSH
                     QIIHCSGWVKIENIDSKGIVSSKTGKYSHSFHSIRRLI"
     gene            2716..4746
                     /locus_tag="P5236_00855"
     CDS             2716..4746
                     /locus_tag="P5236_00855"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HRU62946.1"
                     /translation="MKLNFSKSSLQNIFKILLGSAVVIAIVLLFPKENKFNYHFEIGK
                     PWNYELLTAPFNFPVYKSEEQVIKEKNEILKNYIPYYVLDTTVYETQLKKLLRDNEIQ
                     LNNSVIARNYLREKLQFIYSQGIISSEEYNNLLKNNKTNIYCILPSRVSQIINIDKIY
                     TPQKAYEEIVKNSPEDILSLNLQKYLVENLRYDSITSELSKEDLLKNLSLTSGMVQRG
                     ERIIDKGEIVTPETYAILHSLKIEFDKRNSSIGQSFLVPMGEIIIIVELLILLIFYLN
                     LFRPRIFEKFNNLLFIAILMILIIGITSVVIRYTTLNYYLIPFALLPIIVRVFFDSRT
                     ALFVHIITILIISLMVDNPFQFVLLQITAGMVAVSSLKDMTQRSQLAQTAFYIFLSYT
                     LMYIGFEFITEKEWKRIDWIPILYFAVSCIFLLFAYLLIYIFEKIFGLISAITLIELT
                     NINSDFMMKFAEVAPGTFQHSLQVSNLATEAAKKINANSLLVRTGALYHDIGKMIHPE
                     FFIENQIGNKNPLLEMDYEKAAKTIIQHVTEGVKIAKKNHLPEQIINFIATHHGTSKT
                     KYFYNSFINQFPDIQPNEAAFTYPGPLPNNKETAILMMADAVEATSRSLNSYSEENIE
                     QMVEDIINGQINEGQFRESPISFRDVETVKAVFKEKIKNIYHSRIPYPEVEK"
     gene            4807..5805
                     /locus_tag="P5236_00860"
     CDS             4807..5805
                     /locus_tag="P5236_00860"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005648884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HRU62947.1"
                     /translation="MKTKIAFFDAKSYDIATFNEKNKQYGFEIKYYKSHLNKDNAILS
                     KGADVVCIFVNAEANAEVIDMLVENGVKLIALRCAGYNNVDLKAAKGRIKVVHVPAYS
                     PHSIAEHTVALMLTLNRKIHKAYSRTREGNFALEGLMGFEMNGKTAGIIGTGKIGKIV
                     IQILKSMGMKILAYDAYPDQNFAQSMGIKYTTLNDLYKHSDIISLHCPLTKETEYLIN
                     YDSIKRMKDGVMIINTGRGKLINTKALIDGLKKKKIGYAGLDVYEEESDYFYEDYSNI
                     VIDDDILARLLTFNNVIVTSHQAFFTREALDEIARITLQNIQDFLDGKPLENEISI"
     gene            complement(5877..6320)
                     /gene="rplI"
                     /locus_tag="P5236_00865"
     CDS             complement(5877..6320)
                     /gene="rplI"
                     /locus_tag="P5236_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRU62948.1"
                     /translation="MEVILKEDISNLGYKGDIVKVKDGYARNYLIPTKKAVIATESAK
                     RMLEEDKKQRAHKLERIKNEALELAEKVKDITLVVGAKTSSKGKIFGSVGPIQLAEAF
                     EKAGFTIDRKLIELSEPVKEVGSYKLTLHLHREVTIEIPFEVISE"
     gene            complement(6341..6610)
                     /gene="rpsR"
                     /locus_tag="P5236_00870"
     CDS             complement(6341..6610)
                     /gene="rpsR"
                     /locus_tag="P5236_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRU62949.1"
                     /translation="MANNNEEIRYLNPPTVDIKKKKYCRFKKSGIKYIDYKDPEFLKK
                     FVNEQGKILPRRLTGTSLKYQRRVAQAIKRARHLALLPFVTDLLK"
     gene            complement(6613..6963)
                     /gene="rpsF"
                     /locus_tag="P5236_00875"
     CDS             complement(6613..6963)
                     /gene="rpsF"
                     /locus_tag="P5236_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRU62950.1"
                     /translation="MLNHYETVFILTPVLSDTQIKETVEKFKSLLLENGAVITNEENW
                     GLRKLAYPIQKKTTGFYCLLQFDAEPNVITTLETNYRRDERVIRYLTVRLDKYAYEYA
                     EKRKNVKLKEGKEN"
BASE COUNT         2408 a         1218 c         1098 g         2638 t
ORIGIN      
        1 ttctttacct tttaaatcca ataaaaaaac caattcatcc tgaattccat ctttatttaa
       61 atcgtccagt tgactgctta tttgtttatt atcaacataa acagccaatt gacttctttc
      121 atttgaagaa atgttcttca aattttgcaa ctgaattact acgggtgcat tggcaacaaa
      181 ttttttattg ggattggtca cctttaaatt tatttcgaca gcagcagaag aaacggttgc
      241 tataacaagc aataataaag gaaattgtcg tgcattcatt tttgtaaaat tttaaaagtt
      301 tcctgtaaaa ttagttttct aattgataaa tttcaataat ttttttctat aaaagaggta
      361 aaaattgaca taatatgagt atttttttgt ttttaatttt atttgtcgca ttataagtga
      421 atggaattag ataaaattga gtctttataa taattatccg caattcatta ggggacaaat
      481 tttatttgaa ataatataac gttactttcc caaatgaaca gttgtttcgg caatttcgct
      541 tcgttttaat tatctaacat caatctatct aaattatctt gtaagaattt tttctatgca
      601 actctatact ttccctgttg aactatttga aaatgaataa tttatcacac tgagaacccc
      661 ttatattttt tttcttctat tttactgaag acgtttcttg aaacgtttcc actcgaaact
      721 tcaaacaatt aaaaagtgtt gatttttttt attccgtttt tttaatgaaa aagaatgatt
      781 tatctttgcc ccttcaaacc gagaaagtta agataaatgt taaaaattat cctcaaaggt
      841 attattattg gttggtttat ttcaggaccc attggtccca ttggtatgct ggttattcaa
      901 cgtactatga ataggggtgt caaatatggc attgcaacgg gagtaggtgc tactttttct
      961 gatttaatat atactgttat tactttattt tgcctgaatt ttgttatagg ctttattgaa
     1021 aaaaatcaac tcatcattga acttattggc agtataatag taattttttt cggttatttc
     1081 attttcaaaa gcaatcctgc tcttcaaccc aaacctaacg aacctgccca cgataatctt
     1141 tttaaagatt ttatcagttc ctttgtactt actttttcca accctttgat catttttgtt
     1201 ttgattgctt tgtttgctcg tttgaatttt attactcgag caactaattc tttccttatc
     1261 ctatgcggtt tcctttctat ttttgctggt gcaatgattt ggtggttagc attaactttt
     1321 cttgtaagtc attttcgttc aaaagtcagt attcatcagt tgaaagctat aaatcaaatt
     1381 actggaagca tcattatgtt aattgggctc gttggcactg ttgtaagctt cttaaaataa
     1441 ttcaaccaac tactttaaag gaagtggttg aaaatcagcc tattgaaaat aacaaaactg
     1501 taactttaca aaatagcatc tttgtattgt aaaaagtttt gttagaataa cttagacatt
     1561 tcttcttttg tcattttgtc attttgccat tcaaccagat aatggacatc tcattttttt
     1621 tcctttgtct tgaaaaataa ttttccttta tcttttattt cccctgaaaa agttgcatta
     1681 attacttgaa tttacgttct aaaaaaataa gaaatgatga aattatcaag atgaaaataa
     1741 ccaatgggtt attaaatcaa tcgtctgata gagtgaaacg agtgagaata ttttccagtt
     1801 tttgaagaaa caataccttt tgaatcaatg ttttcaattt taacccagcc agaacagtga
     1861 ataatttgat gagaattgag caaaaggcca acatggacaa ttttcccttc actgttttga
     1921 aaaaaagcca gatcaccggc gtgaacttct ttcaaagaag gaacttgttt acctttttct
     1981 gcttgctgat aagcatttcg tggtaaaaaa atgccacata tggaaaaaat tacttgcgtc
     2041 aatcctgaac aatctattcc taaagcagat tttccgcccc ataaataagg tgaattcaaa
     2101 tattgtttag ccaacgaaat aagctgattc ccagaaggtt taagtggtcg acctgcttca
     2161 tctggattta cttcaaaaat attgttttgt atgtgaaact ttccattttc ataatctgga
     2221 agtaaacttc cgcctggtaa aagcattttt tcatttttgt ttatgatccg aacttctgca
     2281 ataggtacaa gtgtgcaata acaagattca attgattcat tttcaatgga atccaggatc
     2341 tcaatcattt ttctatctat ccaaccaaca gcattatccg ctaaatttct gattttcagc
     2401 cagcgttctc gcgtctcaag aatttcgact cgttcaccaa acaaaagctg cgaatacatc
     2461 tcgctgcatt cattatcttc aactcttaaa ggcaccaaac ttaatgttac aattccttgc
     2521 atttttgact tacattaaaa gcaatattgt atctttattt ttaattcttt atcacaaaat
     2581 tacagaaagt ttataaattt atcttttcat cttgaaatat tttgtaattt tcgccttcta
     2641 attattaata acttcaaact aatttatact ttagtgtttt ctaagttctg ttagaaaaat
     2701 agatactcaa taattatgaa attaaatttt tcgaaatctt cactacaaaa tatttttaaa
     2761 attttattgg gttctgctgt agtaattgca attgttttgc ttttcccgaa agaaaataaa
     2821 ttcaattatc attttgaaat aggcaaaccg tggaattatg aactattgac agctcctttc
     2881 aatttcccgg tttacaaaag tgaagaacaa gtaatcaaag aaaaaaatga aatcttaaaa
     2941 aattatatac cttattacgt ccttgatact accgtttatg aaactcagct taaaaaattg
     3001 cttcgtgaca acgaaattca attaaataac tctgtcatcg caagaaatta cttgagagaa
     3061 aagctacaat tcatttattc tcaaggaatt atttcttcag aagaatataa caatttgctg
     3121 aaaaataata aaacaaatat ttactgtatt ctaccttcga gagtttctca aataatcaat
     3181 atagataaaa tttatacacc tcaaaaggct tatgaggaaa tagtaaaaaa ttcacctgag
     3241 gatattttat cactcaatct acaaaaatat ctggttgaaa atttacgata cgacagtatt
     3301 acttctgaac tttcaaaaga agatttacta aaaaaccttt cccttactag tggaatggta
     3361 caacgaggag aacgcatcat cgataaagga gaaatcgtca cccctgaaac atacgctatt
     3421 ttacattcgc ttaaaattga atttgataaa agaaattctt ctattggaca gtcatttttg
     3481 gtacctatgg gtgaaataat tattattgtg gagctgctaa ttttgctgat attttacctt
     3541 aaccttttcc gtccacgtat ttttgagaaa tttaataatt tgcttttcat cgccatctta
     3601 atgattttga taatcggcat tacctctgtt gtgattcgtt ataccacact aaattattac
     3661 cttattcctt ttgctttgct gcctattatt gtacgagtat tctttgattc ccgtactgct
     3721 ttatttgtgc atattatcac tattctcatc atttcattga tggtggacaa tccttttcaa
     3781 tttgtattgt tgcagatcac cgcaggaatg gttgcggttt ccagcctgaa agatatgact
     3841 caacgttcac aattagctca aacagccttt tatatctttc tgagctatac tttgatgtac
     3901 ataggttttg aatttattac tgaaaaagaa tggaaacgaa tcgactggat acctatttta
     3961 tattttgctg taagttgtat tttcttactt ttcgcttatt tgttgattta catttttgaa
     4021 aagatttttg gattaatttc agctattacg ttgatagaac taaccaatat caatagcgat
     4081 tttatgatga aatttgccga agtggcgcct ggcacttttc aacattctct gcaagtttct
     4141 aaccttgcca ctgaagctgc aaaaaaaatc aatgccaata gtttactggt acgcacggga
     4201 gctttgtatc acgatattgg caaaatgatt catcctgaat tttttattga aaatcagata
     4261 ggaaataaaa atccactttt ggaaatggat tatgaaaaag cagccaaaac aatcattcaa
     4321 catgtgactg aaggggttaa aattgccaaa aagaatcatt tgcctgaaca aattatcaat
     4381 tttattgcca ctcatcatgg cactagtaaa acaaaatatt tttataattc tttcattaac
     4441 caatttccag atatccaacc aaatgaagca gcttttactt atcctggacc gcttcccaac
     4501 aacaaagaaa cagctatcct gatgatggct gatgcggtag aagccacttc aagaagttta
     4561 aactcttaca gtgaagaaaa catagaacaa atggtagaag atatcatcaa tggacaaatc
     4621 aacgagggac aatttaggga atctcccatt agtttcagag atgttgaaac agtaaaagcc
     4681 gttttcaaag aaaaaataaa aaatatttat cattcacgga ttccttatcc tgaagttgaa
     4741 aaataagcta ttgctcaatc atttccacaa acaatacttg taatgtataa ataaataact
     4801 taaataatga aaacaaaaat tgcctttttc gatgcaaaat cctatgacat cgccactttt
     4861 aacgagaaaa ataaacaata tggctttgaa ataaaatatt ataaaagtca tttaaacaaa
     4921 gataacgcca ttctatccaa aggagccgat gtagtgtgca tttttgtaaa tgccgaagcc
     4981 aacgccgaag taatagacat gttggtagaa aacggcgtaa aattaatagc tctccggtgt
     5041 gctggttaca acaatgttga tctgaaagca gcaaaaggac gtatcaaagt ggttcatgtt
     5101 ccagcttatt caccccattc tattgcagaa catacagtag cattgatgtt aactttgaac
     5161 cgaaaaattc acaaggctta ttccagaacg cgtgagggaa attttgcctt ggaaggattg
     5221 atgggattcg aaatgaacgg aaaaacggct ggcattattg ggacaggaaa aattgggaaa
     5281 attgttatcc agattctgaa aagcatggga atgaaaattc tggcttatga tgcttatccc
     5341 gatcaaaatt ttgcccaaag tatgggaata aaatatacta ctttgaatga tctttataaa
     5401 cattcagata ttatttcgtt gcactgtcct ttaacaaaag aaacagagta tctgatcaat
     5461 tacgattcca ttaaaaggat gaaagatggc gtaatgatta ttaatactgg aagaggaaag
     5521 cttatcaata caaaagcatt aattgatgga cttaaaaaga aaaaaatagg ttatgctggt
     5581 ctggatgtgt atgaagaaga aagtgattat ttttatgaag attattccaa tattgttatt
     5641 gatgatgata ttttggctcg actgcttact ttcaataatg taattgttac ttctcatcaa
     5701 gctttcttta ccagagaggc tttagacgaa attgctcgca ttactttgca aaatattcag
     5761 gattttttag acgggaaacc attggaaaat gaaatcagta tttgaaatca aataaaaaac
     5821 cggtaactct tttaagttat cggttttttt atttgctctc tttttaaata tctgacttat
     5881 tcagaaatta cttcaaaagg aatttctatt gtaacttctc tatgcaagtg aagtgtcaat
     5941 ttataggaac caacctcctt aacaggttcg cttaattcta tcaatttgcg gtcgatagta
     6001 aaaccagcct tttcaaaagc ttctgccagt tgaataggtc caaccgaacc aaaaattttt
     6061 cctttagaac tggtttttgc acctactacc aatgtaatat cttttacttt ttctgccaat
     6121 tcaagtgctt cgtttttaat acgttccaat ttgtgtgcac gctgtttctt atcttcttct
     6181 aacattcgtt ttgcactttc ggtagcaatc acggcttttt tggttggaat caggtaattc
     6241 cttgcataac catcttttac tttcacaata tcacctttat aaccaaggtt gctgatatct
     6301 tctttcaata tcacctccat aattttttac cctccagttt ttatttcaat aaatcagtta
     6361 caaaaggaag taaagccaga tgacgagctc tctttatggc ttgggctact ctgcgttgat
     6421 attttaacga agtacctgtt aaacgacgtg ggagaatttt tccttgctcg tttacaaatt
     6481 ttttcaagaa ttcaggatct ttataatcga tatatttaat accacttttt ttgaagcgac
     6541 aatatttttt ctttttaata tcaacggtag gaggatttaa gtatcttatt tcttcgttgt
     6601 tgtttgccat ttttaattct cctttccttc tttaagttta acatttttcc ttttttcagc
     6661 atattcataa gcatatttgt ccaaacggac agtaagataa cgtatcacac gttcatcacg
     6721 acgataattg gtttccaatg ttgttattac atttggttct gcatcgaact gcaaaaggca
     6781 ataaaaacct gtcgtctttt tctggatggg ataagctaat tttcgcaatc cccaattttc
     6841 ctcattggta attacggccc cattttcaag caaaagggac ttaaattttt ctaccgtttc
     6901 ctttatctga gtatcagaca aaacgggagt taaaatgaaa acggtttcat aatgatttaa
     6961 catactgttt attaacgttt taaatttgac atttttctca aaagtgtttg caaagttata
     7021 aataattatt tgatggacaa aaggattcaa cagctttttt aattttctca tagaattgtc
     7081 attggaaaca agttttatga cttaaatgaa aagcatataa aatattgaaa atcattgcaa
     7141 atgttttgtt tttagtacat gacaaaaaga tgatattttc tcttttaaag aagtatttat
     7201 ccgagtaaca acgtgtttgg ctaaaatttt ttttatcaat gataattctt aattcacaaa
     7261 aaagggcagc tcaattgaac tacccttttt gtgaatatag attcttagtt ttttatggcg
     7321 gcttgagctg cagctacccg agcaatagga actctatagg ga
//
LOCUS       DAOTKS010000021         7233 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_9982_length_7233_cov_4.400390, whole genome shotgun sequence.
ACCESSION   DAOTKS010000021 DAOTKS010000000
VERSION     DAOTKS010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7233
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9982_length_7233_cov_4.400390"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="P5236_00880"
     CDS             complement(<1..441)
                     /locus_tag="P5236_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRU62951.1"
                     /translation="MKYYSTNKIVKNITLQEAVVKGLADDKGLFMPEEIKKLPSSFFD
                     TMMNMSLQEISFIVADAFFGDDIDSRALKKIVDDTLNFDIPLIPVEDYIYSLELFHGP
                     TLAFKDVGARFMSRLLSYFIQKEGERNVNVLVATSGDTGSAVANG"
     gene            complement(665..1882)
                     /locus_tag="P5236_00885"
     CDS             complement(665..1882)
                     /locus_tag="P5236_00885"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HRU62952.1"
                     /translation="MKYVVILGDGMADEPIDSLGNKTPLQVANKPTIDKIAALGRSGM
                     LNTIPEGFSPGSEIANLSVLGYDVADVFEGRGSLEAASMGVSLEKGEMAMRCNLICIE
                     NEKIKNHSAGHISNEEAAELIEFLNNKLGNENISFYHGVSYRHLLKMRGGNKNLKCTP
                     PHDVPGIPFRKVMIKALSPDAQATADYLNELTLRSQQLLENHPVNIKRAAEGKDKANS
                     IWLWSPGYKPEMKTLSELYHFKKGSVISAVDLIKGIGVYAGLNVINVEGATGLYNTNY
                     EGKAQAAIEALRTDDFVYVHVEASDEAGHDGDVPLKIKTIEYLDARIVKPIFEEVSKW
                     NEPVTIAVLPDHPTPCALRTHTCDPVPFVIYHPGETPDEVTTYDEFAARKGSYGLLKG
                     NEFMKNLIVNSNL"
     gene            complement(2017..3042)
                     /locus_tag="P5236_00890"
     CDS             complement(2017..3042)
                     /locus_tag="P5236_00890"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HRU62953.1"
                     /translation="MISKIIAEELIEKVYEAINEVDKIAILVHVDPDGDAIGAALGLW
                     HYLMTIEKEPVVISPTSFPNFLSWMPGSKQILIYENDKTSVEKALASAELIFGVDFND
                     RRRLEKMSELVSKILVPTILIDHHLQPEKFAKITISYPEISSTSELIFRLICRMGDFS
                     KINLACAECIYTGMMTDTGNFTYNSNDPEIYIIISELIKIGIDKDEIYRKVFNTYSEN
                     RLKLLGYCLYKKMKIFSTYRTAIISLSQQELIAFNYTNGDAEGFVNIPLSIEGIDFSV
                     LLREEKDKIKISLRSQGNFPANKVAADLFGGGGHLNAAGAESYTSLEETEKQLLNALP
                     DYLIFFD"
     gene            complement(3685..4779)
                     /locus_tag="P5236_00895"
     CDS             complement(3685..4779)
                     /locus_tag="P5236_00895"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HRU62954.1"
                     /translation="MNSIVHFIQQYPFLTIIISFIIGKLFMPVAIKIAKKHNFLVKPN
                     KRTSHKGLIPYTGGLDIFVSFFLTMILFSYEIVAQTQFIILGTLTIFFIGFIDDLITL
                     KPMAKLSSEIIAGIFLIIIANIRISHFHGILGINELPLIMSYLFSLFTFIVIVNALNL
                     IDGVDGLASGLGIIYNLFFGIYFNLSGYLYLALAAYTLAGSLIVFFIYNVFGKKNKIF
                     MGDTGALFLGFMITFFVFEFCEINAYHKVPEAYHMSAAPAVAICLIIVPLFDTFRVML
                     TRIKKGFSPFKADKNHIHHLLLKIGFSHRQVTLILLTSTIFFIFLALIGRNWNNLLLI
                     FIALLIASSLTYYLWRVINKKMQRKTIVNI"
     gene            complement(4871..6832)
                     /locus_tag="P5236_00900"
     CDS             complement(4871..6832)
                     /locus_tag="P5236_00900"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HRU62955.1"
                     /translation="MKDKFSFLKNTDTFSHIVSLKYLPRWIVLLLDVLICCLSFLFAY
                     LITSFFVSDSPDIRVTSFFQRLVLGLSIQIIFFWFFHTYAGILRYSNFTDAIKLLLAV
                     FSNFIVLVIINFIYTKVYSDLLFTYSELIIYAIIAFLFLFLERLFIKVTYDFFVENSS
                     NLVPVMIYGTDPAGISIGKMILSKVGDDNTKYKLVGFIDNDHNTRQKILLGVNVYPYD
                     KNTIATKIPKKAKTIIVSPAKLKEIGFKEDLDVFINNNLSVLTSAPTDLWENEIPNVK
                     QIRSIKIEDLLGREPIHISTTNIENQLKSKIILVTGAAGSIGSEIVHQLVQYQPLLIV
                     LVDNAETPLYELKIELQEKYPQQNFSFFLGDVRDQERMEYIIDLHKPDIIYHAAAYKH
                     VPMMEDFPTESVQVNVKGTKVMADLAVKYKVKKFVMISTDKAVNPTNVMGASKRIAEI
                     YVQSLNKKLQKNGHETTKFITTRFGNVLGSNGSVIPHFQKQIEKGGPVTITHPDIIRY
                     FMTISEASNLVLEASAIGEGGEIYIFDMGEPVKISDLARKMIRIAGYVPDVEIKIVYT
                     GLRQGEKLYEELLNQKEKTIPTQHPKIMVAKVIEYEYDEISLEIEKLLQYSSLNKDFL
                     IVQQMKKIVPEFKSNNSQFEFLDILKKSQ"
BASE COUNT         2586 a         1157 c         1016 g         2474 t
ORIGIN      
        1 accgtttgca actgcacttc cagtgtcacc tgaagtagct accagtacat ttacatttct
       61 ttcaccttct ttttgaataa aatagctcag caaccgtgac ataaatcgtg ctcctacatc
      121 tttgaaagcc agggtaggtc catgaaacaa ttccaaacta taaatatagt cttcaacagg
      181 tataagtgga atgtcaaaat tcaatgtatc gtccacaatt ttttttaatg cccgagaatc
      241 aatatcatca ccaaaaaagg cgtcagcaac gataaaagaa atttcctgta aagacatgtt
      301 catcatggta tcaaaaaaag aagaaggcag tttttttatt tcttcaggca taaaaagccc
      361 cttatcatct gccaaacctt ttaccactgc ttcttgtaag gtaatgttct ttactatttt
      421 gttggtactg taatatttca tctggatatt atttcttttt tccttttggt cgtataagaa
      481 tttgtaagta cttactatat aaaagttatg ttataaaatt attttatgaa ataaatctga
      541 ttttaactaa ttttcctccg aaacaaatat aagaaagttt ttttcatcct tctgaaggtt
      601 tgttagtttt ttaatatttt tgttatttaa aattcataat aattttcaaa ttccttgatt
      661 ttttctataa gttggaattc actattaagt ttttcataaa ttcatttcct tttaataaac
      721 cgtagctccc ttttcgagca gcaaattcat cgtaagttgt cacttcatca ggtgtttctc
      781 caggatgata aatgacaaaa ggaacaggat cacatgtatg cgttctgaga gcacaaggag
      841 ttgggtgatc tggcaaaacg gctatagtaa caggctcgtt ccatttggaa acttcttcaa
      901 aaatgggttt cacaattcgc gcatccagat attcaattgt ttttattttt agtggaacat
      961 caccatcatg accagcttca tcgcttgctt cgacgtgaac ataaacaaaa tcatcagttc
     1021 gtaatgcttc aatagctgct tgggctttac cttcataatt ggtattatat aaacctgtag
     1081 caccttcaac attgataaca tttaaaccag catatacccc aattccttta ataagatcaa
     1141 cagcagaaat gacagaacct tttttaaaat gataaagttc tgataaagtc ttcatttctg
     1201 gtttatagcc tggcgaccag agccaaatgc tgttggcttt gtcttttcct tcagcagctc
     1261 gttttatatt gacggggtga ttttcaagca attgctgaga acgaagcgta agttcattga
     1321 gataatctgc tgtagcttgt gcatcggggg acaaagcttt tatcattact tttcgaaagg
     1381 gaatacctgg cacatcatgc ggtggcgtac attttagatt cttgtttcct cctctcattt
     1441 ttaacagatg gcgatacgaa actccatgat aaaaactaat gttttcattt ccaagcttat
     1501 tattgagaaa ttcaattaat tcagcagctt cttcattgct gatatgtccg gcggaatggt
     1561 ttttgatttt ttcattttcg atacaaatca aattgcatct cattgccatt tctccttttt
     1621 caagagaaac acccatactc gcagcttcta gtgaaccacg cccttcaaaa acgtcagcca
     1681 catcataacc taatacactt aagtttgcaa tttcgcttcc cggagaaaat ccttcgggga
     1741 tagtatttag cattccactt cttcctaagg cagcaatttt atctattgta ggtttattag
     1801 caacttgtaa aggcgtttta ttccctaacg aatcaatagg ctcatcagcc attccatctc
     1861 ctaaaataac aacatatttc atacatttta taataaggct cgataatcaa tagttattac
     1921 agaggttttt atatagtttg ttttgttttt atttctataa agttattcta tgaaaaacag
     1981 tattaaaacc tttcttgttc ataaaaacaa aaaatatcaa tcaaaaaata tcaaataatc
     2041 aggcaaagcg ttcaataatt gtttttcagt ttcttctaaa gaggtataac tttcggctcc
     2101 tgctgcattc aaatgaccac cacctccgaa caagtcggca gctaccttat tggctggaaa
     2161 atttccttgt gaacgcagtg aaattttaat tttatctttt tcttctcgca aaaggacgga
     2221 aaaatcaatt ccttcaatgg aaagcgggat gtttacaaat ccttcagcgt ctccatttgt
     2281 ataattaaat gcaattaatt cttgttgtga aagagaaata atggcagttc ggtatgtgga
     2341 aaaaattttc atttttttgt aaagacaata acccaacaat ttcagacgat tttcagaata
     2401 ggtattaaat actttgcgat aaatttcgtc tttatcaatt cctattttga ttaattctga
     2461 aatgataatg taaatttctg gatcatttga attgtaagta aaattgccgg tatcggtcat
     2521 cattccagta taaatacatt ctgcacaagc taaattgatt tttgaaaaat cgcccattcg
     2581 gcaaatcagt cgaaaaataa gctcactggt tgaagaaatc tctggataag aaatggttat
     2641 ttttgcaaat ttttctggtt gtaaatgatg gtcgattaaa atagttggga ctaaaatttt
     2701 tgatactaat tccgacattt tctctaagcg gcggcgatcg ttaaaatcaa ctccaaaaat
     2761 taattctgca gaagctaaag ctttttccac agaagtttta tcattttcat aaatcaatat
     2821 ttgtttgctg cccggcatcc agcttaaaaa atttgggaaa gaagtaggag aaataactac
     2881 cggttctttt tcaatggtca tcaaataatg ccacaaccct aaagcagctc caatagcatc
     2941 accatcagga tcaacatgga ccaatatggc aatcttatcc acttcattaa tagcttcgta
     3001 aactttttca atcagttctt cggcaataat ttttgaaatc atttatttag gttttaaaaa
     3061 tagatgcaaa agtagtaaaa aaatataagt aggagaattt taattaatac catcacagaa
     3121 ttgacgcatc taattttcca ataatcgtaa aaaaactggt tatcttaacc agcttttaag
     3181 cgtttgtaat cattgaactt ttagacttgt ctttcttcac aaattaaatg taatttttcg
     3241 aagaaaagaa aatttttaag cagcattttt tatgtccttt ttctgcctat ctttattaaa
     3301 aaaaccctat ataataccaa ttgatagtac tttagtataa gcatttttgt aagcaagctc
     3361 aaaacggtaa attatttaag taagctgaat ttatcgaatc gtatgtaaaa aattttaagg
     3421 tacccttcta tgaaaacagg cagcaaactc ggaggaaaaa tagctttcta acagacggat
     3481 aattgtttta gtacaataac aaaaagaaca atttattata agagaaaaaa atttgaactt
     3541 cacacactta tttggaataa tatcattttg gggaaggatg aattaaaaaa tttacatccg
     3601 aacaaagatg tcgacaacag attcaatact tagatgatca actgaaacct tattttataa
     3661 tggagaaaag attcaaataa aatgttaaat atttacaatg gtttttcgtt gcatcttttt
     3721 attaataact ctccaaagat agtaggttaa actgctggca atcaatagag caataaaaat
     3781 taataacaga ttattccaat ttcgtccaat gagagctaaa aaaataaaaa aaatagtaga
     3841 ggtaagcaat attaaagtaa cttgacgatg agaaaaacca atttttaaaa gcaaatgatg
     3901 aatatgattt ttgtcagctt taaaaggaga aaaacctttt tttattctgg ttaacataac
     3961 tcggaaagta tcaaacaaag gaacaataat taaacaaatg gctactgcag gagctgctga
     4021 catatgataa gcttcaggaa ctttatgata agcatttatt tcacaaaatt caaaaacaaa
     4081 aaaagttatc ataaatccta aaaaaagagc tccagtatca cccataaaga ttttattttt
     4141 tttaccgaaa acgttataaa taaaaaatac aatcaatgag ccggcaagag tataagctgc
     4201 taaagccaaa tacaaatagc ctgataaatt aaaatagatg ccaaaaaata aattatagat
     4261 aattcccaaa ccagaagcca aaccatctac tccatcaatt agattcaaag catttacaat
     4321 aactataaaa gtaaaaagtg aaaataaata actcataata agaggtaact cattaatccc
     4381 taaaattcca tgaaaatgag aaattctaat atttgctata ataattaaaa aaattccagc
     4441 aattatttca ctacttaatt ttgccattgg tttcagggta atcaaatcat caataaaccc
     4501 aataaaaaaa atagttaaag tgcctaagat tataaattga gtttgagcta caatttcata
     4561 agaaaaaaga atcatcgtaa ggaaaaaaga aacaaaaata tcaagtccac ccgtataagg
     4621 aattaagcct ttgtgtgaag tacgtttatt aggcttaact aaaaaattat gttttttggc
     4681 tattttaata gcaacgggca taaataattt cccaataata aaagaaataa taatagtaag
     4741 aaaaggatat tgttgaataa aatgtacaat ggaattcatt ttctaaattg atttatgcgt
     4801 gattaaacgg aaagtatgaa aaaaatataa cattttaaaa aagaaggata attaagaaaa
     4861 tatttgttaa ttactgggat tttttaagga tatctaaaaa ttcaaattgt gaattattac
     4921 ttttaaattc aggtacaatt tttttcattt gttgaactat cagaaaatct ttgttaagag
     4981 agctatactg aagtaatttt tcaatttcca gtgaaatctc atcatattca tattcaatga
     5041 ctttagcaac cataatttta ggatgttgtg taggtatagt tttttctttt tgattcaata
     5101 actcttcata taatttttca ccctgtctta agccagtata tacaatcttg atttcaacat
     5161 cagggacata accagctatt ctaatcattt ttcgagctaa atcactaatt tttacaggtt
     5221 cgcccatatc aaatatatat atttctcctc cttctcctat tgcacttgct tcaagcacca
     5281 gattactcgc ttcagaaatg gtcataaaat atcgtataat atcaggatga gttatggtta
     5341 caggtcctcc tttttcaatt tgtttctgga aatggggaat tactgaccca tttgaaccta
     5401 aaacatttcc aaaacgtgta gttataaatt tagttgtttc atggccattt ttctgaagtt
     5461 ttttattcag tgattgaaca taaatttcgg caatccgttt cgaagctccc attacattgg
     5521 ttggattgac cgctttatct gttgaaatca taacgaactt tttaactttg tattttactg
     5581 ctaaatcagc cataacttta gtccctttta catttacttg aacactttct gtcggaaaat
     5641 cttccatcat aggaacatgt ttgtaagctg ctgcatgata aataatatca ggtttatgca
     5701 aatcaataat atattccatc ctttcttgat cccgaacatc ccctaaaaaa aagctaaaat
     5761 tttgttgcgg atatttttct tgaagctcta tttttaattc atacaatggc gtttctgcat
     5821 tatctactaa aacgattaag aggggttgat attgtacaag ttgatgaaca atttcactac
     5881 ctattgaacc tgcggcacca gtgacaagaa ttatcttaga ttttaattga ttttcaatat
     5941 tagtagtaga aatatgaata ggttcccttc ccagcaaatc ttctattttt atggaacgaa
     6001 tttgtttaac attaggtatc tcattttccc agaggtcggt aggagcagaa gtaagaaccg
     6061 acaaattgtt gttaataaaa acatctaagt cttctttgaa tccaatttct tttaatttgg
     6121 caggtgaaac aataatggtt tttgcttttt taggaatttt tgtagcaata gtatttttat
     6181 cataaggata aacgttcact cctaatagta ttttttgccg ggtattatga tcgttgtcaa
     6241 taaatcccac aagtttatat ttggtattat cgtcaccaac tttggaaaga atcattttgc
     6301 caatactgat acctgcagga tcggtaccat aaatcattac tggcactaaa ttgctactat
     6361 tttcgacaaa aaaatcataa gttactttaa taaacaacct ttctaaaaaa agaaatagaa
     6421 aagcaatgat ggcataaata attaactcgg aataagtaaa aagtaaatca gaataaactt
     6481 ttgtataaat aaaatttata attacgagaa caataaaatt actaaaaaca gctaataaca
     6541 atttaatcgc atcggtaaaa tttgaatatc gaagaatacc agcataagta tgaaaaaacc
     6601 aaaaaaatat tatttgaata cttaacccca acactaatcg ctgaaaaaaa gaagtaactc
     6661 gtatatcagg agaatctgat acaaaaaagg aagtaattaa ataagcaaat aaaaaggaaa
     6721 gacaacaaat aagaacatct aataataata ctatccaacg tggtaaatat ttgagagaaa
     6781 ctatatgact aaaagtatct gtatttttta aaaagctaaa tttatccttc attttttgaa
     6841 aatttaatcg tagttaaaac tcatttgccc tttaaaatag tttacaaata tacgtttaaa
     6901 atattaaata tagcttcaat ttctgtatgt tttttatttt gaattttatc tatataaaga
     6961 tatctcaaat gattttaaaa acaacagtga atttttattg caattagtta ctaaaatact
     7021 ccaattccag aaaattgatt tttcaaccta tttttatgct attaaatcat tcaaattttg
     7081 attgatattt tttcattttt tcttaatttt ctttatttta agctattcat tacaacaata
     7141 cttcgttcaa ttttattata aacaattgat aatcaatgat ataaatgtat aattcttgtt
     7201 aaaatatttt agtaagtcat tgggaatcag tat
//
LOCUS       DAOTKS010000022         7210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_10009_length_7210_cov_4.316282, whole genome shotgun sequence.
ACCESSION   DAOTKS010000022 DAOTKS010000000
VERSION     DAOTKS010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7210
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10009_length_7210_cov_4.316282"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="P5236_00905"
     CDS             <1..965
                     /locus_tag="P5236_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRU62956.1"
                     /translation="KKLVEAYQHQQAQGIYKVLIAVGNPENGKILLRIAKKVLEGVKN
                     TLSITVLHITPSTDTNPIYGEQFAEDSFKSIKEEAEILQIPIETVYKVADNVEASIVR
                     IVNQQNFDFLLVGAGLSLSGIPFFKERNIFRKIKWLNKLINKVSQQQVVFYPGSLIKD
                     KTKYFIEQSNCNVGIFVNRGFTDITSTVVLLYDKEDEFLLRYARRILKNNNNATVQIM
                     DVNKVIDNNESVARYINEMEKQFPNAVRILKQNKINSNTFSKYSFMLISYQTWNKLVE
                     IDKNQLANIPTTLIINKKISHFYSDGRRKQIVSLPDNETLNEDN"
     gene            1165..2298
                     /locus_tag="P5236_00910"
     CDS             1165..2298
                     /locus_tag="P5236_00910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRU62957.1"
                     /translation="MKIIIVGTAYPFRGGLAAFNERLAKEFVKEGNEIEIMTFTVQYP
                     DFLFPGKTQFSSEKPPEGLKITRRINSIDPFNWRKVGKEIKSKSPDKVIFSYWTSFIA
                     PCFGTIAKFAHSPKTKMIGLIHNMISHESTFLDRWLPSYFVKYMDGFVAMSQTVIKDI
                     RLFDKQNKPIAISPHPIYDHYGELVPKKEAAAKLGLHEQKHYILFFGFIRYYKGLDLL
                     LEAFADKRLEELDVHLIIAGEFYEHPEPYLKQIQRLNLENKVELRSNFIPDNDVKYYF
                     SIADLIVQPYRSATQSGISQIAYHFEKPILVTNVGGLAEIVPHQKVGYVVEVNPQKIA
                     DAIIDFYTNNRSEEFINNIKVEKSKYSWSGMTSTINMLFEETQ"
     gene            2330..4942
                     /gene="mutS"
                     /locus_tag="P5236_00915"
     CDS             2330..4942
                     /gene="mutS"
                     /locus_tag="P5236_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005849895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRU62958.1"
                     /translation="MQENIVETPLMKQYFKIKAKHPDAILLFRCGDFYETYSSDAIEA
                     SEILGITLTRRANGAAKSVEMAGFPHHALDTYLPKLVRAGKRVAICDQLEDPKLAKTI
                     VKRDVTELVTPGVTYNDTVLNFKENNFLASVYISKSQIGVSFLDVSTGEFLVAEGNGE
                     YVDKLLNSFSPKEILFDRNRKKEFEDLFGTKFYTYPLDDWVYTPESAYERLLKQFETT
                     SLKGFGVANLSNGIVAAGAILHYLDITHHQQTAHITHLSRIEEEQYVWLDRFTVKNLE
                     LFESINEGGRSLCDIIDKTISPMGARLLKRWIAFPLKNVQQINERLNVVEYFFKHPDV
                     KLQIEENIALIGDLERLVSKVAVGRINPREVVQLKVALKAIEPIKKICSATDNVTLKT
                     IGDQLNPCSIISERIEKEILNDPPILLNRGNIIQKGIDQELDELRELAYSGKDFLQKI
                     QERESKATGIPSLKIGFNNVFGYYLEVRNTHKDKIPAHWIRKQTLVNAERYITEELKI
                     YEEKILGAEEKIIAIETKLFNDLIISLLDYIPAIQLDSNLIAQLDCLLSFAKVSADNR
                     YMKPEINDDDIIEIKQGRHPVIEKQLPLGETYIPNDIYLDSNTQQIMMITGPNMSGKS
                     ALLRQTALITLMAQMGCFVPAESAKIGVVDKIFTRVGASDNISLGESTFMVEMNEAAS
                     ILNNLSNRSLVLFDELGRGTSTYDGISIAWAIVEYIHEHPDHRAKTLFATHYHELNEM
                     EKSFSRIKNYNVSVKEIDNKVIFLRKLVRGGSEHSFGIHVAKMAGMPQSIVKRAEQIL
                     KQLETDNRQSDIAKPVDKIAQQREGYQLTFFQLDDPVLEQVRDEIKNLDINNLTPLEA
                     LNKLNEIKRIVTGK"
     gene            5302..5781
                     /locus_tag="P5236_00920"
     CDS             5302..5781
                     /locus_tag="P5236_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005825798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRU62959.1"
                     /translation="MEVFNMALPRLFTSRSNWTPSLPSLIDRFFDSDLMDWDTFNFAG
                     ENMTLPAVNVKENDNEFKIEVAAPGLNKEDFKVNYDNGILTISSEKQEEKEEKEGEKV
                     TRREFSYQSFQRSFSVPEDIINADKISAKYDNGILYVTLPKHEEAKKAKNVKEIKIS"
     gene            complement(5928..>7210)
                     /locus_tag="P5236_00925"
     CDS             complement(5928..>7210)
                     /locus_tag="P5236_00925"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRU62960.1"
                     /translation="RQKEGVPTLNMDWWNYGGITRDVMLITVPEIYIEDYAVQYKTDK
                     NGTIYGWIKLNKPLSDQNLFFEIPELKIKQSLTTNEEGIVNFEIKSHPKLWSPTSPKL
                     YEVKFSTDKETFSDQIGFRTIETKGKEIFLNGKKVFLKGISIHEEAPFRQGRAWSKED
                     AKILIDWAKELGCNYVRLAHYPHNENMVREAERAGLMVWSEIPVYWTISWGNSETYKN
                     AERQLHDMIYRDKNRCAVIIWSIANETPQSIERDLFLSNLSKYAKSQDSTRLISMAME
                     VTSSNDNVYTVKDNMNQYVDIISFNEYIGWYNGTPDLCRLVKWEIPYNKPVIISEFGG
                     GALPGLYGDKSERWTEEYQEELYLKNLEMLEKIDGLAGISPWILVDFYSPRRQLYGIQ
                     DFFNRKGLISNNGIKKKAFYILQEWYKKIDEKYK"
BASE COUNT         2492 a         1172 c         1186 g         2360 t
ORIGIN      
        1 aaaaaaagct cgtagaagca tatcaacatc agcaagcaca gggaatttac aaagttctta
       61 ttgctgtagg taatcctgaa aatggaaaaa ttttacttcg aatagctaaa aaagtattag
      121 aaggtgtcaa aaatacttta tctattactg tcttgcatat tactcccagt accgatacca
      181 accctattta cggagaacaa tttgcggaag atagttttaa gtcgataaag gaagaagcag
      241 aaatacttca aattcctatc gaaacagttt ataaagttgc ggataacgtt gaggcatcaa
      301 ttgttcgaat agtcaatcag caaaatttcg attttttgct cgtaggtgca ggactttctc
      361 tttcaggcat tccatttttt aaagaaagaa atattttcag aaaaataaaa tggcttaata
      421 aacttattaa taaagtttct caacaacagg tggttttcta cccaggcagt ttgataaaag
      481 acaaaactaa atactttatc gaacaaagta attgcaatgt aggaattttt gtaaacagag
      541 gttttacaga tattacttca accgtagtgt tgctttatga taaagaagac gaattcttat
      601 tacgctatgc aagacggatt ttaaaaaaca acaacaatgc aacggttcaa attatggatg
      661 tgaataaagt gattgataac aacgaaagtg tggctcgata cataaatgag atggagaaac
      721 aatttcccaa tgcagtcaga attcttaaac aaaacaaaat aaattccaat actttttcaa
      781 aatacagctt tatgctgatt agttaccaga cctggaataa acttgtagaa atagataaaa
      841 atcaacttgc caatattcca acaactctca ttatcaataa gaaaataagt catttttata
      901 gtgatggacg tcgcaagcaa attgtttcgc ttcctgataa tgaaacttta aatgaagaca
      961 actaaaagga atccagcaat ttatttttat aaatttaaaa atgactatta caatttaaat
     1021 tgaaaataaa atagctttta aattttatcc taatttcctt tcaaaatttg taactttgtg
     1081 acaatctcgt ggcttgcaac aatctttttt acaaaaatat cagcaaaagc tatcattgca
     1141 acatcataaa tcaaaattta tccagtgaaa atcattatag taggaacggc ttatcctttt
     1201 cggggaggtt tggcagcatt taacgaacgt ctggcaaaag aatttgtcaa agaaggaaat
     1261 gaaatagaaa ttatgacctt taccgttcaa tatcctgatt ttttattccc tggaaagact
     1321 caattttcca gcgagaagcc tcctgaagga ttaaaaatca ctcgacgcat caattctatc
     1381 gatcctttca attggagaaa agtgggcaaa gaaattaaaa gtaaatcacc tgacaaggtc
     1441 atttttagtt attggacttc ttttattgca ccttgttttg gaaccattgc taaatttgcg
     1501 cattctccaa aaacaaaaat gattggactc attcacaaca tgatttctca cgaaagtacc
     1561 tttttggata gatggttgcc ttcttatttt gtcaaatata tggatggttt tgttgctatg
     1621 tcacaaaccg ttataaaaga cattcgctta tttgataaac aaaacaagcc aattgccatt
     1681 tcaccgcatc caatttatga tcattatggt gaattggttc ccaaaaagga agcagctgca
     1741 aaattaggac ttcatgaaca aaaacattat attttatttt ttggatttat acgatattac
     1801 aaaggattgg acttgctttt agaggctttt gctgataaaa ggttggaaga attagatgtt
     1861 catttgatta ttgctggtga attttatgaa catcctgaac cttatttaaa acaaattcaa
     1921 cgtcttaatt tagaaaataa agtagaatta cgttcgaatt ttattcctga caatgatgtt
     1981 aaatattatt tcagtattgc tgacctgatt gtgcaaccat atcgttcagc aacgcaaagc
     2041 ggcatcagtc agatagctta tcattttgaa aaacctatat tggttaccaa cgttggggga
     2101 ttagctgaaa ttgttcctca tcagaaagtt ggttacgttg tcgaggtaaa tccccaaaaa
     2161 attgcggatg ccattataga tttttatact aataaccgtt cagaagaatt catcaataac
     2221 ataaaagtag aaaagtcaaa atattcatgg tcgggaatga catcaactat caatatgcta
     2281 tttgaagaaa cgcaatagtt agaaaataat tttttgagaa actaagaata tgcaagaaaa
     2341 cattgtagaa acacctctaa tgaaacagta ttttaaaatc aaggcaaagc accctgatgc
     2401 tattttattg tttcgttgcg gtgattttta tgaaacttat tcctctgatg ctattgaagc
     2461 ttcggaaatt cttggtatca cccttacccg acgtgccaat ggtgccgcta aaagtgtaga
     2521 aatggcggga tttcctcatc atgctctcga tacttatttg cccaaattgg ttagagctgg
     2581 taaaagagta gctatttgcg accaactgga agatccaaaa cttgctaaaa ctatcgtaaa
     2641 aagagatgtc acagagttag taacaccggg cgttacttat aatgatactg tattaaattt
     2701 taaggaaaat aatttccttg ccagtgttta tatcagcaaa agtcagattg gcgtttcttt
     2761 tcttgatgtc tcaacaggtg aatttttagt agccgaaggt aatggagaat acgttgataa
     2821 actcttaaat agtttttcac ccaaagaaat tcttttcgat cgtaaccgaa aaaaagaatt
     2881 tgaagatttg tttggaacaa aattttatac ttatccatta gacgattggg tttatacacc
     2941 tgaatcagct tatgaacgat tgttgaagca gtttgaaacc acaagcctga aaggattcgg
     3001 tgttgctaat ctttccaatg gcattgttgc tgcgggagct atacttcatt atctggacat
     3061 tacccatcat caacagacag cacatattac tcatctttca cgaattgaag aagagcaata
     3121 tgtctggctc gaccgcttta cggtaaaaaa tttagaactt ttcgaaagca taaatgaagg
     3181 tggacgttcc ctttgcgata ttatagataa aaccattagc ccaatgggtg ctcggcttct
     3241 gaaacgctgg atagctttcc ctcttaaaaa tgttcagcaa attaacgagc gtttaaatgt
     3301 cgtagaatat ttttttaaac atccagatgt aaaattgcaa attgaagaaa atatagcttt
     3361 aataggtgat ttagaacggc tggtatcaaa agtagccgtt ggcagaatca atccgcgtga
     3421 agtggtacaa ctgaaagtgg ctttgaaagc tattgaacct attaaaaaaa tttgttcagc
     3481 tactgataat gtcacgttaa aaactattgg tgatcaatta aatccgtgtt caattatttc
     3541 tgaacgtatt gaaaaagaaa tcctaaatga tccgcccatt ttgttgaatc gtggcaatat
     3601 tattcaaaaa ggtattgatc aagagctgga tgaattacgt gaattggcat attctggaaa
     3661 ggacttttta cagaaaatac aagaacgtga aagcaaagct actggtattc caagcttaaa
     3721 aattggtttc aataatgtgt ttggttatta tcttgaagtc agaaacactc ataaagataa
     3781 aataccagca cactggattc gaaaacaaac attggtcaat gcagaacgtt acattacaga
     3841 agaattaaaa atttatgaag aaaaaatttt gggagcggaa gaaaaaataa ttgctattga
     3901 aacaaaattg ttcaatgatt tgataattag cttgcttgat tacattccgg ctattcaatt
     3961 agattccaat ctgattgctc agttggattg tttgctttct tttgcaaagg tttctgctga
     4021 taatcgttat atgaaacccg aaattaatga tgatgacatt attgaaatta agcaaggaag
     4081 acatcccgtc attgaaaaac aattgccact gggagaaacg tatatcccga atgacattta
     4141 ccttgacagc aatacccagc aaattatgat gattactgga cccaacatgt caggcaaatc
     4201 agctttgctc cgacaaactg ccttgattac tttaatggct caaatgggat gttttgttcc
     4261 agcagaaagt gcaaaaattg gtgtagtgga taaaattttt acgcgtgttg gagcaagcga
     4321 taacatctcg ttgggagaat ctacttttat ggtggaaatg aacgaggctg ccagcatttt
     4381 aaacaatctc tcgaatcgaa gtctggttct ttttgacgaa ttaggaagag gaacaagtac
     4441 ttatgatgga atttctatcg cctgggcaat tgtagaatat atccatgagc atcccgatca
     4501 tcgtgctaaa acactttttg ccacacacta tcacgaatta aatgaaatgg aaaaatcatt
     4561 ttcacgtatt aaaaactaca acgtttcagt aaaagaaata gataataagg taattttttt
     4621 acggaaatta gttcgtggcg gtagtgaaca cagttttggt attcatgtgg ctaaaatggc
     4681 gggcatgccc caaagtatcg ttaaacgagc tgaacagatt ctcaaacaac ttgaaaccga
     4741 taaccgtcag tcggatattg ccaaaccagt agataaaata gctcaacaac gcgaaggtta
     4801 tcaacttaca ttttttcaac ttgatgaccc tgtattagaa caagttcgag atgaaattaa
     4861 aaatcttgat ataaataatt taacacctct cgaagcctta aacaaattaa atgagataaa
     4921 acgaattgtt acaggaaagt aaactgaagt ctttttttga tttataatgt tttacactgc
     4981 aattgggtcg tgataaaaaa agaaagtaat tagtaaaaag ccttttattt tattttattt
     5041 tattttttct ttctattaca aacctgtcaa tctgacattc ataattttat tttaaaaaga
     5101 caagttgtct tttctcttat ttgattgtta aataattttt atgtttgaga acgcttttta
     5161 atttatatat aactgaaaat caaccactta tttttctgcc taatagattt attcttttgc
     5221 tggtataggt tttgcaaagt aacaaatgaa atcaacaagg atttcaaaaa gtaattatta
     5281 gatgtttaac ttaattaaaa aatggaggtg tttaatatgg cattaccaag attatttaca
     5341 tcacggagca attggactcc ttctttacca tcgttaattg acagattttt tgatagcgat
     5401 ttaatggatt gggatacttt caactttgcc ggtgaaaaca tgacattacc agcagtaaac
     5461 gtaaaagaaa acgacaatga atttaaaatt gaagtagctg ctcctggttt gaacaaagaa
     5521 gattttaaag tcaattacga taatggtatt ttaactattt cttctgaaaa acaagaagaa
     5581 aaagaagaaa aagaaggaga aaaagtaaca cgtcgtgaat ttagctatca atcattccaa
     5641 agaagcttca gcgtacctga agatatcatt aatgctgata aaatttcagc aaaatatgac
     5701 aatggaattt tgtatgtaac tttacctaaa cacgaagagg caaagaaagc taaaaatgta
     5761 aaagaaatta aaatctcata atttcttctt acttctcaaa agaggaagtt taagtaaaaa
     5821 gcttcctctt ttttatataa actttttgtg aagggggtac taaaaagagc agttttaaaa
     5881 aataatttct gtctgatttt ttccattaaa atttcacaat tttcatttca tttatatttc
     5941 tcgtctattt ttttatacca ttcttgcagg atataaaaag cttttttctt gataccattg
     6001 tttgaaatca aacctttccg attaaaaaaa tcctgaattc catataattg acggcgagga
     6061 gaataaaaat ctaccaaaat ccatggagaa attcctgcaa gtccatcgat tttttccagc
     6121 atttcgaggt tttttaaata aagctcttct tgatattctt ctgtccatct ttcactttta
     6181 tctccatata aacctggtaa agctcctcca ccaaattcac taataatgac aggtttatta
     6241 taaggaattt cccattttac caaacgacat aaatctggtg tgccattgta ccatcctata
     6301 tattcattaa aactaataat atctacatat tgattcatat tatcttttac agtataaaca
     6361 ttgtcattgc tggaagttac ttccatagcc atacttatta agcgcgtact atcctgcgat
     6421 ttagcatatt ttgataagtt acttaaaaat agatctcgtt caatgctttg aggagtttca
     6481 ttagctattg accaaataat tactgcacaa cgatttttat cgcgataaat catatcatgt
     6541 aattgtcgtt cagcattttt ataggtctca gaatttcccc acgatatagt ccaataaaca
     6601 ggaatttcag accagaccat caatcctgca cgttcggctt cacgaaccat attttcgtta
     6661 tgcggataat gagccagacg aacataatta cagcctaact cttttgccca atcaattaat
     6721 atttttgcat cttcttttga ccaggctctt ccttgtcgaa aaggtgcttc ttcatgaatg
     6781 ctgattcctt tcagaaaaac ttttttaccg tttagaaaaa tttcttttcc tttcgtttca
     6841 atagtgcgaa atcctatttg atcagaaaat gtttctttat cagtactgaa ttttacttca
     6901 tatagtttag gagaggtagg tgaccataat ttgggatgtg attttatttc gaagttaact
     6961 attccttcct catttgtagt taaagattgt tttattttca attcaggaat ttcaaaaaat
     7021 aaattttggt cacttaaagg tttgtttaat tttatccatc cataaattgt tccgttttta
     7081 tctgttttgt actgaaccgc gtaatcttca atataaattt ctggtactgt tatcagcata
     7141 acgtctctgg taattcctcc ataattccac cagtccatgt ttaaagtagg aactccctct
     7201 ttttgtcttt
//
LOCUS       DAOTKS010000023         6995 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_10339_length_6995_cov_4.151009, whole genome shotgun sequence.
ACCESSION   DAOTKS010000023 DAOTKS010000000
VERSION     DAOTKS010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6995
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10339_length_6995_cov_4.151009"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="P5236_00930"
     CDS             complement(<1..357)
                     /locus_tag="P5236_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HRU62961.1"
                     /translation="MGKEIERKYLVKGDFKPWIKNSYTIIQGYLCSVPERTVRIRVKN
                     QEKGFITIKGKTEKNGLNRYEWEKEIPLQDAQELLQLCEPGIIEKTRYEVIFKGQRFE
                     IDEFHGENEGLIIAEIE"
     gene            complement(440..>2504)
                     /locus_tag="P5236_00935"
     CDS             complement(440..>2504)
                     /locus_tag="P5236_00935"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRU62962.1"
                     /translation="SQQETLSDKKIELIAVPSLVGQAKQTYELLDKLYPSDTSDHEWI
                     KTAVVLPNETLLTPLLYSLPPQIKKVNVTMGLPLNITPVQGLLEHIFELQRQMRITDN
                     RYLFYHQTVSNLLNHQYITLLCGREAQEIQTTVIENNLIYVDADAFENDELLATIFTP
                     QEDTKNFLTYLLQILVILQKKFQQISETSQSYQLEYNFTSQYYLTLRRLNEVIKENAS
                     AIEMSLDTLMRLICQITASLTIPFVGEPLEGLQIMGVLETRGLDFDNLIITSFNEDFF
                     PKKTTPNSFIPYNLRRGFGLPTIEHQEAIFSYNFYRLIHHAKHIFLLYDSRTEGLQKG
                     EVSRFLYQLQYHYKVPFTTKNVLYDISFKNSPVLQIEENASVLEKLKSFLNNQPENRA
                     LSASSINTYIDCPLQFYLTYVEGLKEADEVKETIEYDMFGTLFHSSMEFLYQPFKNKL
                     VNSSDLDTILKDSAQIEIAIQKAFSEKYFNKREDYQNIKLEGNYLLVANVLKKYIIQL
                     LKIDKFYTPFKYLGSEKEFQLEFPIQNGQEKVNLKGIIDRIDEKEGRLRIIDYKTGNG
                     SLTFKNIEEVFAHEANNRPKYILQTFLYSMLYKYKSNTSLPIQPEIYYIKNLFKNDFT
                     SQLTLKPNKEETIIIEDFNFYEEEFKTYLITCLEEIFNPAISFYQATNNKLCQYCPYQ
                     SICDR"
     assembly_gap    2505..2514
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2515..3330)
                     /locus_tag="P5236_00940"
     CDS             complement(<2515..3330)
                     /locus_tag="P5236_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRU62963.1"
                     /translation="MSSFLYRIAEIYYSQYQEKISEFTFVFPNRRAGIFFQHYISEIA
                     QKPIFSPEILTIEECFSSASSLLTADQISLIFLLYQIYRDISKSNESFDTFVFWGEML
                     LNDFDEIDKHLVNPQQLFTNVTELKQIDQQFKTFSEEQIKAIKQFWKNFIPFPAEKVQ
                     ENFISIWKILYLLYKEFRSALFKKSLATEGMIYRWVAEKMLNGEIILQWENKPFVFIG
                     FNALNACEKTLFSYLKNKNQADFYWDYEAAELRDENNPASRFYKENTQMFPSKF"
     assembly_gap    3578..3677
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3678..4723)
                     /locus_tag="P5236_00945"
     CDS             complement(<3678..4723)
                     /locus_tag="P5236_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016271016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HRU62964.1"
                     /translation="MNKEINVKLVMRMLGSLLLSEATALLLVFFLSFVFKESDGYYFL
                     ITSGVCYLIGLLGILLGRNASQEMGKREGSLVVTLAWVIFSLFGALPFFISKNIPSYT
                     DAFFETISGFTTTGATILNNIEELSHTMLFWRSFTQWMGGLGIIVIFLALLPMFGTSG
                     AQLFAAEASGPTKDKIHPKITETAKRLFLIYFLLTFAETILLRIGGMNWFDAVCHSFT
                     TIATGGFSTKQNSIAYWNSPFIQYIIILFMILAGVNFSLYYFGFKNKFKKIKENEEFR
                     YFLIVLFSFAIIIFISLIDFSQPVSFHKIENSWRDALFTITSLMTTTGFTIVDYMQWA
                     PFAWIILLIVMLTGA"
     gene            complement(4809..6143)
                     /gene="trkA"
                     /locus_tag="P5236_00950"
     CDS             complement(4809..6143)
                     /gene="trkA"
                     /locus_tag="P5236_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HRU62965.1"
                     /translation="MKIIIVGAGEVGTHLAKLLARENMEITLLDENPERLKDLDLNYD
                     LLTYEGSPTSINNLKEIGVENADLFIGVTPYESVNMTACMIATNLGAVKTLARINNYE
                     YLLPQNKEFFKKLGVDYLIYPEVLAANEIVESLKTSWLRQNMSFCNNALILLGIKVRS
                     NAVIINKKFSTGFFDHNRYRVVAIKRGSQTIIPKGSDEILANDIVYFITTPENLEFVK
                     EQAGKEDFPIRNVMFMGGSRIAQKTLFNLPSHIHAKVLERDKEKSLLLANKFPNALII
                     NADARNIDIIKQEGIEEADAFVAATSNSEANILACLVAKQFGVKKTIAEVENLDYIPL
                     AESLDIGAVINKKMIAASYIYQITLHANVLNVRNLTAADAEVVEFVAKPTSKITRSKI
                     KDLKLPENVNIGGIVRNGIGSIVNGDTIIKPDDHVIVFCISSSIRKIEPFFN"
     gene            complement(6231..>6995)
                     /locus_tag="P5236_00955"
     CDS             complement(6231..>6995)
                     /locus_tag="P5236_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008154522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRU62966.1"
                     /translation="LQRAYDNIIHDVALQNLHVVLCLDRAGLVGPDGPTHHGVFDLAY
                     LRCIPNLTIAAPRNEIELRNLMYTAQQKDSGPFAIRYPKGKGNIIDWKKPMNILPIGK
                     GECLKEGKNLAILSVGMIAANVEKAIEKVESEHHISIAHYDMRYVKPLDEDLLHEIGK
                     KFKQIITIEDGVIQGGFGSAISEFFSNSNDRPKIQRLGIPDTFVEQGSIEELQTLLGL
                     DAESIAKTIVEILKVNGKEKTKRKLKPNIMFGKKII"
BASE COUNT         2354 a         1159 c         1032 g         2340 t
ORIGIN      
        1 ttctatttcg gcaatgataa gtccttcatt ttcgccatga aattcatcaa tttcaaaacg
       61 ttgaccttta aaaattactt cataacgagt tttttcaata atccctggtt cgcatagctg
      121 aagcaattcc tgagcatctt gcaaaggaat ttctttttcc cattcataac gatttaatcc
      181 atttttttct gtttttccct tgatggtaat aaatcctttt tcttgatttt tgacacgaat
      241 acgaacagtt cgttctggta cagaacataa atatccctga ataatagtat aactattttt
      301 aatccatggc ttgaaatcac cttttaccaa atatttacgt tcaatttctt ttcccatttc
      361 aaaaaacaga aaacttgatt ataataatgc aaatttatga aataaattta aggatactcc
      421 agattttttt aacttattgc tacctgtcgc aaatagactg atatgggcaa tattgacaaa
      481 gtttattatt tgtagcttga taaaaagaaa tggctggatt aaaaatttct tccaaacaag
      541 taattaaata agttttaaat tcttcttcgt aaaaattaaa atcttcaata atgatagttt
      601 cctccttatt aggtttcaaa gtcaattgcg aagtaaaatc atttttaaac agatttttta
      661 tgtaatatat ttctggctgt attggtaatg aagtgttgga tttgtattta tacaacatac
      721 tatacaagaa agtttgcaat atatattttg gacgattatt tgcctcgtga gcaaaaactt
      781 cttctatatt tttaaaagtc aatgagccat ttcctgtttt ataatcaatg attcttaaac
      841 gtccttcttt ttcgtcaatt ctgtcaatga tgccttttaa gtttactttt tcttgtccat
      901 tttgtatggg gaattcaagt tgaaattcct tttcagagcc aagatattta aagggagtat
      961 aaaatttgtc gattttcaat aactgaataa tatatttttt aagcacattt gccaccaaaa
     1021 gataattacc ttccaattta atattttgat aatcttctct tttgttaaag tatttttctg
     1081 aaaaagcttt ctgaatagca atttcgattt gtgccgaatc ttttaatatg gtatctaaat
     1141 cagaagaatt gaccaactta tttttaaaag gctgataaag aaattccatc gaactgtgaa
     1201 acaatgtccc aaacatatca tattcaatag tttcctttac ttcgtcagct tcttttaatc
     1261 cttctacata agtgagataa aattgaagtg gacaatcaat ataagtattg atagaacttg
     1321 ccgaaagagc acgattctca ggctgattat tgaggaaaga tttcaatttt tcaagaacag
     1381 aagcattttc ctcaatttgt aaaacaggtg aatttttgaa actaatatcg tataaaacat
     1441 ttttggtagt aaaaggaact ttgtaatgat attgcaattg atacaaaaac cgactcactt
     1501 ctcctttttg aagtccttct gtacgtgaat cgtaaagtaa aaaaatatgt tttgcatgat
     1561 gaatcaatcg gtaaaaatta taggaaaaaa tagcttcttg atgttcaatg gtaggtaaac
     1621 caaatccacg acgtagatta taaggaataa agctattggg tgtagttttt ttaggaaaaa
     1681 aatcctcatt aaaggaggtg ataattaaat tatcaaaatc caaccctctg gtttccagaa
     1741 cgcccataat ttgtaagcct tccaatggct caccaacaaa tggaatggta agtgaagcag
     1801 taatttgaca tatcaaacgc atcaaagtat ccaagctcat ttctatcgca gaagcatttt
     1861 ccttgattac ttcattcaaa cgtcgaagag tgaggtaata ttgacttgta aaattatact
     1921 ctaactgata actctgagaa gtttccgaaa tttgctgaaa ttttttctga aggatgacaa
     1981 gaatctgtaa caaataagtc aaaaaatttt tcgtgtcttc ctgaggagta aagatagttg
     2041 ccagcagttc atcgttttca aatgcgtctg catcaacata aattaaattg ttctctatta
     2101 cagtagtttg aatttcttga gcctcacgac cgcaaagtaa cgtaatatat tgatgattta
     2161 aaagattgga aacagtttga tgataaaata aatatcgatt atcagtgata cgcatttgtc
     2221 gctgtaattc aaaaatatgt tccaaaagcc cttgcactgg tgtaatgttt aatggcaatc
     2281 ccattgtaac atttactttt tttatctgtg gtggcaaaga ataaagcaat ggcgtcaaca
     2341 aagtttcgtt gggcaaaacg acagcagttt ttatccattc gtggtccgaa gtatccgaag
     2401 ggtaaagttt gtcgagcaat tcataggttt gctttgcttg tcccaccagc gaaggaacgg
     2461 ctattaactc gattttttta tctgataaag tttcctgttg agaannnnnn nnnnaaattt
     2521 tgaaggaaac atttgcgtgt tttccttata aaaacgagaa gcaggattgt tctcatcacg
     2581 aagttcagca gcttcataat cccaataaaa atcagcttga tttttattct ttaaatatga
     2641 aaaaagtgtc ttttcacaag catttaaagc attaaaacca ataaaaacaa acggtttgtt
     2701 ttcccattga agaataattt caccatttag cattttttca gctacccaac gatatatcat
     2761 tccttcagta gccaggcttt ttttgaataa tgctgaccga aattccttgt aaagtaggta
     2821 aagaattttc cagatagaaa tgaaattttc ctgaactttt tcagcaggaa aaggaataaa
     2881 gtttttccaa aattgtttta tagcttttat ttgctcttca gaaaaagttt tgaactgttg
     2941 atcaatttgt ttcagctcag taacattggt aaataattgt tgaggattaa caagatgttt
     3001 atcaatttca tcaaaatcgt tgagtaacat ttcaccccag aaaacaaaag tatcaaaact
     3061 ttcatttgat ttgctgatgt cacgataaat ttgataaagt aaaaaaatca agctgatttg
     3121 gtctgctgtt agaagtgaag acgctgaaga aaaacattct tcgatagtaa gtatttcagg
     3181 tgaaaaaatt ggcttttgtg ctatttccga aatataatgc tgaaaaaaaa tacccgctct
     3241 acggttgggg aacacaaaag taaattcact gattttttcc tgatattgag aataataaat
     3301 ttctgctatg cgataaagga atgaagacat ttataatttt ttaaggataa aaaaacagta
     3361 aaaatataaa attaaaactt tctcatttaa aatttgtaat tttataattg atagagcgtc
     3421 tttccgtgtt tttcaaacaa tggttttgaa aaaatcaaat aaatagagaa aaatcttatt
     3481 ttttccagaa ggcaggagta aaaagaacta atacagtgaa aagttccaaa cgtcccagca
     3541 acataataaa tgataaaaac catttgctaa aaactggnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnngca cctgtcagca ttacaattaa caaaataatc cacgcaaaag
     3721 gtgcccattg catatagtca acaatagtaa atcctgtagt agtcatcaac gaagtaatag
     3781 taaataaagc atctctccaa gaattttcta ttttatgaaa agaaacaggt tggctaaaat
     3841 cgattaaaga aataaaaata ataatggcaa aagaaaaaag aacgattaaa aaataacgga
     3901 attcttcatt ttcttttatc tttttaaatt tatttttaaa accaaaataa tacaagctaa
     3961 aattcacacc tgcaagtatc ataaataata tgatgatgta ttgaataaat ggagaattcc
     4021 aatacgctat actgttttgt ttggtagaaa agccaccagt tgcgatagtg gtaaatgaat
     4081 ggcaaacagc atcgaaccaa ttcatccctc caatacggag taaaattgtt tcagcaaaag
     4141 tcaataaaaa gtaaattaaa aacaatcgtt tggcggtttc agtaattttt ggatgaattt
     4201 tatctttggt tggtccgctt gcttctgctg caaaaagttg agccccactt gttccaaaca
     4261 tgggtaaaag agccaaaaaa ataacaatta ttcccagccc acccatccac tgagtgaaac
     4321 ttctccaaaa caacatagta tgtgataatt cctcaatatt gtttaaaata gtagcacctg
     4381 tagtagtaaa accggaaatt gtttcaaaga aagcatcagt gtaagaagga atatttttac
     4441 taataaagaa aggcaaagca ccaaacaagg aaaaaataac ccaggcaagt gttaccacca
     4501 atgaaccctc ccgcttcccc atttcctgag aagcatttcg tcctaataat attcctaaaa
     4561 gaccaattaa ataacaaaca ccagaagtta taagaaaata atagccatcg ctttccttaa
     4621 aaacaaatga aagaaagaaa acaagaagaa gagcagtcgc ttcacttaat aataaacttc
     4681 ccagcatccg cattactaac ttcacattga tttctttatt catagtcttg gaatttagct
     4741 atttaagttt attatctcat gttaaacttt tttcaacagt gtgggaaaat atattatcta
     4801 aagctctatt agttaaaaaa gggttcaatt tttcgaatag atgaagatat acaaaataca
     4861 atcacatgat catccggttt gataatagta tcaccattga caatactgcc tatgccattt
     4921 ctaacaattc ccccaatatt tacattttca ggtaatttta aatctttaat ttttgaacgg
     4981 gtgatttttg atgttggttt agccacaaat tccactactt ctgcatctgc tgctgttaaa
     5041 ttgcgaacat tgagcacatt tgcatgtaag gtaatttgat aaatataact ggcagcaatc
     5101 atctttttgt taattactgc accaatatcc aaactttcag ccagaggaat gtaatccaga
     5161 ttttcaacct ctgcaatagt tttttttact ccaaattgct ttgctaccaa acaagctaaa
     5221 atattggctt cagaattgga agttgctgcc acaaaagcat ctgcttcttc aattccttct
     5281 tgtttaataa tatcaatatt tctggcatca gcattaataa ttaatgcatt aggaaacttg
     5341 ttcgccaaca acaaactctt ttctttatct ctttcgagca ctttggcatg gatatgggaa
     5401 ggaagattga ataaggtttt ttgtgcaatt cggctacctc ccataaacat tacatttcga
     5461 attggaaaat cttctttccc tgcttgttct tttacaaatt ccagattctc aggagttgtg
     5521 atgaaataaa caatatcatt agcgagtatt tcatcggaac cttttggaat gattgtttga
     5581 cttcctcgtt tgatggcaac aactctgtaa cggttatgat caaaaaagcc tgtgctaaat
     5641 tttttattta tgataacagc attactgcga acttttatgc ctaaaaggat aagagcatta
     5701 ttgcaaaaac tcatgttttg acgcaaccaa cttgttttca aagattctac aatttcattg
     5761 gcagctaaca cttctggata aattaaataa tcaaccccca actttttgaa aaattcttta
     5821 ttttgtggta ataaatattc gtaattattg attcttgcca atgtttttac agcacctaaa
     5881 ttggtagcta tcatacaggc agtcatattc acactttcat aaggagtgac gccaatgaaa
     5941 agatcagcat tttcaacgcc aatttccttt aagttattaa ttgaagttgg tgatccttca
     6001 taggtcaaaa gatcgtagtt caaatctaag tcttttaatc gttctggatt ctcgtcgagc
     6061 aaagtgattt ccatattttc ccgcgctaac aattttgcca aatgggtacc aacttcacct
     6121 gctccaacaa taataatttt catttttgta aatttaatag gagagaaata agctactgaa
     6181 aaattaaagg tttaattttt aaaaaatcat tttatcgtaa atctttgctt tcaaattatc
     6241 ttttttccaa acattatatt tggtttcaat tttcgttttg ttttttcttt tccattgact
     6301 ttcagaattt caacaatagt tttggcaatg ctttcagcat ccaagcctaa caaagtttgc
     6361 aattcttcta tgcttccttg ctcaacaaag gtatcaggaa ttcccagccg ttggattttc
     6421 ggcctatcat tactattact gaaaaattct gaaatggcgc ttccaaaacc tccttgtatc
     6481 actccatctt caatagttat gatttgttta aatttttttc caatttcgtg taataaatct
     6541 tcatctaaag gttttacata acgcatgtca taatgagcaa tagaaatatg atgttcactt
     6601 tcgacttttt cgatggcttt ttctacgttg gctgctatca ttcctactga taaaatggct
     6661 aaatttttcc cttctttcaa acattcacct tttccaatcg gtaaaatatt cattggcttt
     6721 ttccaatcaa taatatttcc ttttcctttt ggatagcgaa tagcaaaagg tccagaatct
     6781 ttttgttgag cagtgtacat taaattacgg agttcaattt cattacgagg ggcagcaatt
     6841 gttaaattag ggatgcatcg gagataagcc aaatcaaaaa ccccgtgatg agtaggacca
     6901 tctggtccta ccagcccagc tctatcgagg cataaaacca catgcaaatt ttgaagagca
     6961 acatcatgaa taatattatc gtaagctctt tgcaa
//
LOCUS       DAOTKS010000024         6846 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_10587_length_6846_cov_4.524665, whole genome shotgun sequence.
ACCESSION   DAOTKS010000024 DAOTKS010000000
VERSION     DAOTKS010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6846
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10587_length_6846_cov_4.524665"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..578
                     /gene="rimP"
                     /locus_tag="P5236_00960"
     CDS             111..578
                     /gene="rimP"
                     /locus_tag="P5236_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HRU62967.1"
                     /translation="MISEKTIHQLAEKFTENKDFYIVELKVNPDNQILIKFDSFNGVT
                     IDDCVSLSEFIESNLDREIEDYELEVSSPGLTEPFKVLQQYEKNIGNEVEVLTKGGNK
                     FSGKLLNATNNEFVVQIKKMKKLENSKRKTIVTENVIFHYDEIKTTKYILRFK"
     gene            583..1842
                     /gene="nusA"
                     /locus_tag="P5236_00965"
     CDS             583..1842
                     /gene="nusA"
                     /locus_tag="P5236_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRU62968.1"
                     /translation="MAKRETLNLIDSFSEFKDLKNIDRSTLISVVEESFRNIIAKKYG
                     TDENFDVIINPDKGDFEIYRNRTIVADENFQDPNLEIPLSEAQKIDEDYEIGEEVTDT
                     VDFMDFGRRAILTLKQTLSSKISDLHKENIYIKYKEKVGQIVTAELYQIWKNEMLLLD
                     EEGNELYLPKSEQIPTDFYRKGDMVRAVVAMVENRNNIPRITLSRVSPVFLERLFELE
                     IPEIHDGLITIKKIARIPGERAKVAVESYDDRIDPVGACVGIKGSRIHGIVRELRNEN
                     IDVINYTNNISLFIARSLSPAKISSIRINEEEKKAEIYLKPEEISQAIGKGGLNIKLA
                     SMLTGYTLDVFRELDEAETEDIYLDEFSDEIDEWVIDVLKSIGCDTAKSVLSISRDDL
                     IHRTDLEEETVDNVLTILKAEFEEEEK"
     gene            1912..4992
                     /gene="infB"
                     /locus_tag="P5236_00970"
     CDS             1912..4992
                     /gene="infB"
                     /locus_tag="P5236_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRU62969.1"
                     /translation="MPIRLSKACKDLNVGMNTAIDFLAKKGHKLTADPNVKLTDEMFL
                     LLAKEFNKDMALKIESERISQERQLKEKQETVALDDFIHHKPAEKEIETIPTTIPADQ
                     VPHLKSVGYIDLEEQIKKSKEKKEEVISQTSAKEKELTTKQKIQLKEEKLPETIIPEK
                     EKVTNIETTVSPKIELEEIKKEKTTVEKKEKILVAEVEEKEKTVEPKKISSKIKAESA
                     EKVEKQQISHEVEEKNLIGREEIFTAKIEETAENEEENEIFEIAHPILDTNLTVIGTI
                     DLDAINQSTRPKSKSKEQKKKEYAEKNRLLNESKKFAPSKSKKIIKLSEEETDEVELD
                     EFKEQHKKKRARFTKQKVDIAKIFPNGTSISTLEPPKKVVSPKVGKGKLKKTIKPAIS
                     EEEVQKQIKETLARLSAASSKKGKSAKYRRDKREMLNARQQELAQREQEEEKVLKLTE
                     FVTVNELANLMDVTVNDVIATCMNIGMIVSINQRLDAETINILADEFGYKVEYISQDI
                     LESVFEEEPDKEEDLQPRPPIVTVMGHVDHGKTSLLDYIRKTNVIAGEAGGITQHIGA
                     YNVMLDNGQRITFLDTPGHEAFTAMRARGAQVTDIAIIIIAADDSVMPQTVEAINHAT
                     AANVPIVFAINKIDKPGVNPEKIKEELANMNYLVEEWGGKYQSQDISAKTGIGVPELL
                     EKVLLEAELLDLKANPKKRASGSVIESSLDKGRGYVATVLVQNGTLHQGDIVLAGIHY
                     GKVKAMFNERNQHITEAKPSEPALILGLNGAPQAGDNFIVMPSEHEAREIATKREQLQ
                     REQTIRTKKHFTLDDLGRRIALGNFKELNIIVKADVDGSVEALSDSLLKLSSDKIQVN
                     IIHKAVGAISDSDVLLAAASNAIICGFQVRPTSSARKLAEKEEIDIRLYSIIYDAIEE
                     IKSAMEGMLSPEIKEEVTATLEVRDVFKISKVGSVAGCFVREGKIKRTNKVHLIRDGI
                     VIYTGEIASLKRFKEDVKEVSANYECGISIQNFNDIHVGDIIESFEETEIKSTL"
     gene            5181..5252
                     /locus_tag="P5236_00975"
     tRNA            5181..5252
                     /locus_tag="P5236_00975"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5214..5216,aa:Glu,seq:ttc)
     gene            5285..5539
                     /gene="rpsT"
                     /locus_tag="P5236_00980"
     CDS             5285..5539
                     /gene="rpsT"
                     /locus_tag="P5236_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRU62970.1"
                     /translation="MANHKSALKRIRQANKKRLHNRYYAKTARNAVKKLRTTTDKTTA
                     TEMLPKVTSMLDKLAKRNIIHKNKASNLKSKLTIYVNKLS"
     gene            complement(5652..6305)
                     /gene="upp"
                     /locus_tag="P5236_00985"
     CDS             complement(5652..6305)
                     /gene="upp"
                     /locus_tag="P5236_00985"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HRU62971.1"
                     /translation="MQVVNFSDTNSILHHFLKEIRSVEIQKDSMRFRKNMERIGEIMA
                     YEISKTMSYVSETVQTPFGISPVNLINEKIVISTILRAGLPFYQGFLNFFDGAESAFA
                     SAYRKYKNAVDFDIYVEYIASPSIEGKTLILTDPMLATGSSMEMTYNTLLAKGKPTKI
                     YIAAVIASQPAINYVVSHFPEEKTSIWAAAIDPELNEHSYIIPGLGDAGDLAFGEKI"
     gene            complement(6372..>6846)
                     /locus_tag="P5236_00990"
     CDS             complement(6372..>6846)
                     /locus_tag="P5236_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRU62972.1"
                     /translation="RLDKNYPVGLFKGYYRNEELTREAFRNNTYHTGDLAWRDEDGYF
                     WFVGRTDDVIKSSGYRIGPFEVESALMTHPAVVECAVTGIPDELRGQVVKATIVLSKE
                     YKEKAGPALIKEIQDHVKKVTAPYKYPRVIEFVDELPKTISGKIRRVEIREKDSQ"
BASE COUNT         2552 a         1103 c         1228 g         1963 t
ORIGIN      
        1 ataattcttt ggatttaaat aaaatatttt gtatttttgc atcgaattat tgaattgtgg
       61 aggggactta aaaagtcccc tcatttatta aatgaatatg ctgaaaggaa atgataagcg
      121 aaaaaaccat tcatcaactg gcagaaaaat ttactgaaaa taaggatttt tacatagtag
      181 agttgaaagt taatccagat aatcaaatct taataaagtt cgattctttc aatggtgtca
      241 ctattgatga ttgtgtcagt ctcagtgaat tcatcgaatc gaatctcgac cgtgaaatag
      301 aagattatga attagaagtc agttcacctg gcttaactga gccatttaag gttttacaac
      361 aatatgaaaa aaatattggg aacgaagtag aagtactgac taaaggagga aataaatttt
      421 caggaaaact cttgaacgca accaataatg aatttgttgt acagattaaa aaaatgaaaa
      481 aactcgaaaa ttccaaaaga aaaacaatag taactgaaaa tgttattttt cattatgatg
      541 aaataaaaac aaccaaatat attttaagat ttaaataaca ccatggccaa aagagaaaca
      601 cttaatttaa ttgatagctt ttcagaattc aaagacctga aaaatataga cagaagcaca
      661 ctaatcagtg tagttgaaga atcgttccga aatattattg caaaaaaata cggtacggac
      721 gaaaattttg atgtgatcat taatcctgat aaaggtgatt ttgaaattta ccgaaatcgt
      781 actattgtag ctgatgaaaa ttttcaggat ccgaatcttg aaattccgct cagcgaagct
      841 caaaaaattg atgaagatta tgaaattggt gaagaagtca ccgatactgt tgattttatg
      901 gatttcggac gaagagctat tttaacatta aaacaaacat tatcctctaa aatttctgat
      961 ttacacaaag agaatattta tatcaaatac aaagagaaag ttggacaaat agtcaccgca
     1021 gagctttatc aaatctggaa aaatgaaatg ctacttctcg acgaggaagg aaatgaactt
     1081 tacttgccaa agtccgaaca aattccaact gacttttacc gcaagggaga tatggtacgt
     1141 gccgtggtgg caatggttga aaataggaac aatattcctc gtatcaccct ctcgcgtgta
     1201 tcacccgttt ttttagaacg tttgtttgaa ttagagattc ctgaaattca cgatggattg
     1261 attactatca aaaaaattgc cagaatccca ggtgagcgag caaaagtagc agtagaatct
     1321 tacgatgata gaattgaccc tgtgggtgca tgtgtaggaa taaaaggatc gcgtattcac
     1381 ggcattgttc gggaacttcg gaacgaaaat attgatgtga taaattatac gaacaacatc
     1441 agcttattta tcgccagatc attaagtcca gcaaaaatat cttctattag aataaatgaa
     1501 gaagagaaaa aagctgaaat ttatttaaaa ccagaagaaa tttcacaagc aatcggtaaa
     1561 ggtggattga acattaaact tgctagtatg ttgacaggtt atacactcga tgttttccgt
     1621 gaactggatg aggcagaaac agaagacatt tatctggacg aattcagtga tgaaattgac
     1681 gagtgggtaa ttgatgtttt aaagtcaatt ggctgcgata ccgctaaaag tgtattgtct
     1741 atttcacgtg acgatttgat tcacagaacc gatttagaag aggaaacagt tgataatgta
     1801 ctgactatat tgaaagctga atttgaagaa gaagaaaaat aagaatttta tttttaacaa
     1861 aataattcag cagaatcgtt ataaaaaatt ttaaaaacaa agaaataaaa tatgcccata
     1921 agattaagca aagcttgtaa agatttgaat gtaggcatga atactgccat tgatttttta
     1981 gcaaaaaaag gtcataaatt gacagctgat ccaaatgtga aactcacgga tgagatgttt
     2041 cttttacttg ctaaagaatt taacaaggac atggccttga aaatcgagtc agaacgaatc
     2101 agtcaggaac gacagttaaa agaaaaacaa gaaacggtcg ccttggatga ttttattcat
     2161 cataaaccag ccgaaaaaga aattgaaacg attcctacaa ccattcctgc tgatcaagtg
     2221 cctcatttaa agtccgtagg ctatattgat ttggaagaac aaataaaaaa atcaaaagaa
     2281 aaaaaggaag aagttatttc acaaacttca gctaaagaaa aagagctcac aacaaaacaa
     2341 aaaattcaac ttaaagaaga aaaactaccg gaaactatta ttccggaaaa agaaaaagtt
     2401 actaatattg aaacaactgt ttcaccaaaa attgaattag aagaaataaa aaaagaaaaa
     2461 acaactgttg aaaaaaaaga gaaaattctt gtagccgaag tggaagaaaa agaaaaaact
     2521 gttgagccta aaaaaatatc ttccaaaata aaagctgaat ctgcagaaaa agttgaaaaa
     2581 caacaaatca gtcatgaagt tgaagaaaaa aatctcattg gaagagaaga aatattcact
     2641 gctaaaatag aagaaactgc tgagaatgaa gaagaaaatg aaattttcga aattgctcat
     2701 cctattttag atacaaactt aaccgtaata ggaacgattg atctggatgc catcaatcaa
     2761 agtacccgtc ccaaatcaaa atctaaggaa caaaaaaaga aagaatatgc tgaaaaaaat
     2821 aggttgctga atgaatcgaa aaagtttgct ccttcaaaaa gtaaaaaaat aattaagtta
     2881 tccgaagaag aaactgatga agtagaactg gatgaattta aagaacaaca taaaaagaaa
     2941 cgtgctcgtt ttacaaaaca aaaagtagat atagctaaaa ttttccccaa tggcacttct
     3001 atttcaacat tagaacctcc taaaaaagtg gtttcaccaa aagttggcaa gggaaaactt
     3061 aaaaaaacaa taaaacctgc catttcggaa gaagaagttc agaagcaaat caaggagacg
     3121 ttagccagac tttcagcggc ttccagcaaa aaaggcaaaa gtgccaaata tcgaagagat
     3181 aaacgtgaaa tgctaaatgc ccgtcaacag gaacttgctc aacgggaaca agaagaagaa
     3241 aaagttttga aattaacgga attcgtcact gttaacgaac tggcaaattt gatggatgtg
     3301 actgtgaatg atgttatcgc aacctgtatg aacattggaa tgattgtttc cattaatcaa
     3361 cgactggatg cagaaaccat taatatactt gctgatgaat ttggttataa agttgaatat
     3421 attagccagg atatattaga gtctgttttt gaagaggaac cagataagga agaagattta
     3481 caaccccgtc cacctattgt tacggttatg ggacacgtgg atcacggaaa aacttcttta
     3541 cttgattaca tcaggaaaac caatgtaatc gctggtgaag ccggaggaat cacccaacat
     3601 attggtgctt ataatgtgat gctcgataac ggacaacgaa tcaccttcct cgatacgcct
     3661 ggacacgaag cttttacagc catgcgtgca cgcggtgcac aagttactga tattgccata
     3721 attattattg ctgctgatga tagcgtgatg ccacaaacgg ttgaagctat taatcatgct
     3781 acggctgcta atgtccctat cgtttttgct attaataaaa ttgataaacc gggagttaat
     3841 ccagaaaaga ttaaagaaga attggcaaat atgaattatc tggtagaaga atggggagga
     3901 aaataccaat cacaagatat ttcagcaaaa acaggaattg gtgttcctga attgctggaa
     3961 aaagttctgc tggaagctga attacttgat ttaaaggcaa atccaaaaaa acgtgcttct
     4021 ggttctgtga ttgaatcttc gctcgacaaa ggtcgcggtt atgttgccac tgttttggta
     4081 caaaatggaa cgcttcatca aggagatatt gtgttggctg gtattcatta tggaaaagtg
     4141 aaagccatgt tcaatgaacg aaatcaacac attaccgaag ctaaaccttc tgaaccagct
     4201 ttgattttag gtctaaatgg cgcacctcaa gcaggtgata actttattgt tatgccatcg
     4261 gaacatgaag cacgtgaaat tgctacaaaa cgtgagcagt tgcaaagaga acaaacaatc
     4321 cgaactaaaa aacattttac attggatgat ttaggaagaa gaattgcact gggcaacttt
     4381 aaagaactga atattattgt gaaagcagat gtagatggtt cagtagaagc cttgtcagat
     4441 tctttactta aattatcgag cgataaaatt caggtaaaca taatccataa ggctgttgga
     4501 gcaatttctg attcagatgt gttgcttgct gctgcctcta atgctattat ttgtggattt
     4561 caggtacgtc ctacttcttc agcacgtaaa ctggcagaaa aagaagaaat tgacatccga
     4621 ctttattcta ttatttatga tgccatagaa gaaataaaat cagctatgga aggtatgctt
     4681 tcccctgaaa ttaaggaaga ggtgaccgct acactcgaag ttcgagatgt atttaaaatt
     4741 tcaaaagttg gatcagttgc aggttgtttt gtacgagaag gtaaaatcaa acgaactaat
     4801 aaagttcatc ttattcgcga tggaattgta atctacactg gggaaattgc ttcattaaaa
     4861 cgtttcaaag aggacgtaaa ggaagtaagt gcaaattacg aatgcggtat cagtattcag
     4921 aattttaatg atatccacgt aggtgacatt attgaaagtt ttgaagaaac agaaattaaa
     4981 tctacacttt gagagttttt acctctccat taatttatta agctgagcta agatatcaac
     5041 ttatttccac atcgggttaa taaagcattg attgttatag gaataaaaag atttttaagt
     5101 aaaaactttc gtaatccaaa aaactacatt atctttgcag ccgcaattcc tcttttggaa
     5161 ttgtttttaa tataagtgat ggcccattcg tctatcggtt aggacgcaag attttcattc
     5221 ttgaaagagc agttcaattc tgctatgggc tacaaaatag ataaacaaat tatttaaaaa
     5281 aattatggca aatcataaat cagcattgaa aagaattcgt caagccaaca aaaaaaggtt
     5341 gcataaccgt tattatgcaa aaacggcaag gaacgctgta aaaaaactcc gtacaacaac
     5401 cgataaaaca acagctactg aaatgctacc taaagtgact tctatgctcg acaaactggc
     5461 aaaaagaaat attattcata aaaataaagc cagtaatttg aagtcaaaac tgactattta
     5521 tgttaataag ttatcgtaaa aaagaccgtt tcatttaata aaaaactctt tatctttttt
     5581 ggaaggataa agagtttttt tattacttca aatgaggttt cttctcttgt ttttttctga
     5641 taattctcta attatatttt ctctccgaag gctaaatcac ccgcatcacc taaaccgggt
     5701 ataatataag aatgttcgtt caactcagga tcaatagctg ctgcccaaat gctagttttt
     5761 tcttcaggga aatgagaaac gacataattt attgctggct gacttgcaat gacggctgca
     5821 atataaattt tagtcggttt ccctttagca agtaaggtat tgtacgtcat ctccatcgaa
     5881 ctgcctgttg ccagcattgg atcagtgaga ataagtgttt ttccttctat agaaggtgaa
     5941 gcaatatatt ctacgtaaat atcaaaatca acagcgttct tatatttgcg ataagcagag
     6001 gcaaaagcac tttctgctcc atcaaaaaaa ttcagaaatc cttgataaaa aggtagtccg
     6061 gctcttaaaa tagtggaaat gacaattttt tcatttatca aattaacagg agaaattcca
     6121 aaaggagttt ggacagtttc acttacataa gacatagttt tactaatctc gtaagccatt
     6181 atttcaccaa ttctttccat gttttttcta aaacgcatac tgtctttttg aatctctact
     6241 gaacgaattt ctttcaaaaa atgatgaaga atcgaattcg tatccgaaaa gtttacaact
     6301 tgcataatat attgattaac aaatatttaa aaaacacaag attcaatttc cagttaatat
     6361 ataatctctt tttattgact gtctttttct ctgatttcaa cgcgtcgaat ttttccacta
     6421 atagttttag gtaattcatc cacaaattca ataacacggg gatatttata aggagctgtt
     6481 acttttttta catgatcctg aatttctttt atcaaagctg gtcctgcttt ttctttatat
     6541 tctttggata gcacaatcgt tgcttttact acttgtccac gaagctcatc agggattcca
     6601 gtcacagcac attctactac agcaggatgt gtcatcaagg cactttctac ttcaaatggt
     6661 ccgattctgt agccagaact tttgataacg tcatctgtgc gaccaacgaa ccagaaatac
     6721 ccatcttcat cacgccatgc caaatcacct gtatgataag tattattacg aaaagcttca
     6781 cgcgtcagtt cttcattccg gtaataacct ttaaacaatc ccactggata attcttatcc
     6841 aaacgg
//
LOCUS       DAOTKS010000025         6842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_10595_length_6842_cov_3.640194, whole genome shotgun sequence.
ACCESSION   DAOTKS010000025 DAOTKS010000000
VERSION     DAOTKS010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6842
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10595_length_6842_cov_3.640194"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..1119)
                     /locus_tag="P5236_00995"
     CDS             complement(199..1119)
                     /locus_tag="P5236_00995"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HRU62973.1"
                     /translation="MKYLKKLLILGMLLPTFLFASNIQKTTKTFAVKDEEELKMDIYT
                     VDSIFSSTQPCLIFAFGGGFKEGKRDEPAYQPYFDYFTKKGFIVVSIDYRLGMKEQKA
                     PGVFNTKPLENAVSLAVEDLFSATKYLLENVEELNIDKKRIIISGSSAGAITVLQAEY
                     ELKNHHSLASGLPDDFSYAGVISFAGGIFSTEGLPSYSIPPAPTLFFHGSADKLVPYK
                     ETRFFNKGMFGSKSLTRNFYKKNYPYLFYSMEGLGHEVASFPMKEFLPEIEKFIKIFV
                     FEKKLWMVEVYFTDKQRKSEINIAPSNYYK"
     gene            1380..2594
                     /locus_tag="P5236_01000"
     CDS             1380..2594
                     /locus_tag="P5236_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HRU62974.1"
                     /translation="MPLKVDTKAEKQRSLFPEERLLKEIAKQPHLSIGIPKENQEIET
                     RLALTPEGVAIVTEEGHSVYIERGAGEAMSYSDLQYSEAGAFLVDGKEEAFGTDLVLK
                     ITPPTAKELSYMSDRSSIFSMLQLSNFSAEIIKLMISKKMNAIAYELIRDEQKGYPVV
                     SSISEIEGNTAISLIAELMSNAHGGKGILLGSVAGITPPEIVILGASITGSVAARTAL
                     ALGAQVKIFDYDINKLRKIQQHLGQQIFTSVIHPKVLFKAFTTADAVIGNLRYINGSE
                     RFMVSEDLVKTMKQGAIIIDLSMDQGGCFETSESRTLQNPIFEKYGIIHYCIPNISAR
                     VARTASMALSNIFAPILLKIGHSGNVDAAITESYGFRHGVYIYNGILVNRLIGEYFGI
                     PSNDINLLLPRY"
     gene            2709..5009
                     /locus_tag="P5236_01005"
     CDS             2709..5009
                     /locus_tag="P5236_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HRU62975.1"
                     /translation="MSEEVNIQQVKEDVPLNEEVNLQQMKEEELIQKEFEGLLEDYRN
                     SNHRQKVEIITKAFNFAKTAHKGMRRHSGEPYILHPLAVARIVVKEIGLGSTSICAAL
                     LHDVVEDTDFTVDDIRNHFGDKIAKIVEGLTKISGGVFGEKASTQAENFRKLLLTMSD
                     DIRVILIKIADRLHNMRTLDSLPPAKQYKIAGETQYIYAPLAHRLGLFRIKTELENLS
                     FKYEHPDIYKSIEEKLSIDEEERKKFYKELSVPIQEKLTSMGYEFTLQERVKSVYSIW
                     HKMLTRNIPFEEVYDILALRIIFKPKEGISEKDQCWMIYSAITEIFPPHPERIRDWLS
                     IPKANGYEALHVTVMGPGGRWVEIQIRTERMHEIAEKGLAAHWKYKLKETEETELDKW
                     LKTIKELLEHPDTDAINFLDNFKLNLFASEIFVFTPKGEIRTIAQGATAFDFAFLIHS
                     ELGMHCIGAKVNHKLVPLSYKLQSGDQVEILTSQKQMPQQEWLNYVVTAKAKTAIEEY
                     LKKEERKIMLEGQKLTEKILAEINLSISNENINKILKHYNLTQPNEFYYQAGKGVLNL
                     DEIKNIVFKPKKPQKTISKYLKIPFISTSNDKKAEVTSTEEKIDLKGTLKLTEENVGK
                     KYKLAECCHPIPGDDILGYVDDDNSIIIHKRQCPVAAKLKTNFGERIVSVEWATHKIL
                     SFKEKLEIKGIDRKGILIEILKVISENYESNISELNVTTNDGIFTAQFQLFVHDTEEI
                     ENICENLKRIPEIQKVQRIQELENSI"
     gene            complement(5019..5612)
                     /gene="ruvC"
                     /locus_tag="P5236_01010"
     CDS             complement(5019..5612)
                     /gene="ruvC"
                     /locus_tag="P5236_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRU62976.1"
                     /translation="MKGAQIILGIDPGTTIMGYGLLKVTASDPELMVMGVLELKKYRQ
                     HYVKLQQIFKRTLSLIDEFHPDCMAIEAPFYGKNVQSMLKLGRAQGVAMSAALYRNIP
                     ITEYAPLKIKMAITGNGRASKEQVADMLRRFLKISPENMLPQLDATDGLAAAYCHYLR
                     MGKPLPEKKYTNWKDYVAKNGERVILSTSGKFQKKDF"
     gene            5842..5915
                     /locus_tag="P5236_01015"
     tRNA            5842..5915
                     /locus_tag="P5236_01015"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5876..5878,aa:Ala,seq:ggc)
     gene            6229..6531
                     /locus_tag="P5236_01020"
     CDS             6229..6531
                     /locus_tag="P5236_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU62977.1"
                     /translation="MPTILDLVKVPYPATYQGNKIQPLEGVSLRQAFLTGKQDIQGYQ
                     FWEHSNKRAVTKGKWKAVSKIGTDNWELYDILTRTSQNQTSFSKLDKNISFCNVCQ"
BASE COUNT         2474 a         1079 c         1103 g         2186 t
ORIGIN      
        1 ataaggcaaa gctgaagtaa ccagcgtacg tttaaattgc ttcatatttt aaattctatc
       61 tcactttttg caaaatattt tgcaaatata gtgacaaaaa ctcaaaattc ctgaatttcc
      121 taacgcgttt atccttttat aaaattaaaa ttgaatatat caatcacaat aaaataattg
      181 ataatgaatt cattataatt atttataata attacttggt gcaatgttta tttcagattt
      241 gcgttgtttg tctgtaaaat aaacttcaac catccatagt tttttttcaa atacaaaaat
      301 tttgataaat ttctcaattt caggcaaaaa ttctttcata ggaaagctgg caacttcgtg
      361 ccccaagcct tccatactat aaaaaagata gggataattt ttcttataaa aattacgggt
      421 taatgatttg gaaccaaaca tacctttgtt aaaaaaacga gtttctttat aaggtaccag
      481 cttatcagca ctgccatgaa aaaaaagagt tggtgcaggt ggaattgagt aagaaggtaa
      541 accttcggta ctaaaaatgc cgcctgcaaa ggaaatcaca cctgcataac tgaaatcatc
      601 aggaagccct gaggctaaag aatgatgatt tttgagttca tattctgcct gaagaacagt
      661 aatggcgcct gcacttgaac cactgataat tatacgtttt ttgtcaatat ttaattcctc
      721 tacattttca agtaaatatt ttgttgcaga aaacaaatct tcaaccgcta aagaaacagc
      781 attctccaaa ggtttggtat taaaaactcc gggcgctttt tgctctttca tccccaatcg
      841 ataatcgatt gaaaccacaa taaaaccttt ttttgtaaaa taatcaaaat aaggttggta
      901 ggcaggttca tctcttttcc cttctttgaa gcccccacca aaagcaaaaa tcagacaggg
      961 ttgagtagaa gaaaatatgg aatcgacagt ataaatatcc attttgagtt cctcttcatc
     1021 ttttacagca aaagttttgg tagttttctg aatatttgat gcaaaaagga atgttggtaa
     1081 caacattcct aaaattagta atttttttaa atatttcatt ttttaatata attatttgtt
     1141 atacattaac ttaaacctaa cctattaaaa agttaaagca aacatttttc ttacaaaaat
     1201 atggaaaaag aaaattatta gatagtggat tcaataaaaa attgtaatat tgcttgagat
     1261 ttttccgaat tctccttaat gagaaaacta aaactattgt tttataataa atatagttgc
     1321 attttttgct catttaaaga atagtaatta gttttgtaaa tcaaagtaaa aaaaatttca
     1381 tgccactaaa agtagatact aaagccgaga aacaacgttc gttatttcca gaagaacgcc
     1441 ttttaaaaga aatagcaaag cagcctcatt tgtcaattgg aattccgaaa gaaaatcaag
     1501 agatcgagac gcgtttagct cttacaccag agggagtagc tattgttaca gaagaaggac
     1561 atagtgttta cattgaaaga ggagctggcg aagccatgtc gtactctgat ttacaataca
     1621 gcgaggctgg cgcttttttg gtagatggga aagaagaagc ttttggaaca gatttagttt
     1681 taaaaattac gcctccaacc gcgaaagaat taagttatat gagcgacaga tcaagtattt
     1741 tttctatgtt gcaattaagc aatttttcag ccgagataat taagctcatg ataagcaaaa
     1801 aaatgaatgc tattgcttat gaattaattc gtgatgaaca gaaaggctat ccagttgtaa
     1861 gttccatttc agaaattgaa gggaatactg ctatttctct tattgccgaa ttaatgagca
     1921 atgcacatgg agggaaggga atattattgg gaagtgttgc gggtattacg ccaccagaaa
     1981 tagtcattct tggagcaagt atcacaggtt ctgtagcagc aagaactgct ttggcattgg
     2041 gggcacaggt gaaaattttt gattacgaca ttaataaatt gcgaaaaatt caacaacatc
     2101 tcggacaaca gatttttact tctgtcattc atcccaaggt attattcaag gcatttacta
     2161 ctgccgatgc tgtcattgga aatcttcgtt atataaatgg ttctgaaaga tttatggttt
     2221 ctgaggattt agtaaaaaca atgaaacagg gagctatcat tattgattta agtatggatc
     2281 aaggaggttg ttttgaaact tcagaatcca ggactcttca aaatcctatt tttgaaaaat
     2341 atggaattat tcattattgc attccaaata tttcagctcg tgtagcacga accgcctcta
     2401 tggctttaag taatattttt gcccctattt tgttaaaaat tggtcattca ggaaatgtag
     2461 atgcagctat tactgaaagc tatggtttcc gacatggggt ttatatttat aatggaattc
     2521 tggtaaatag gctgattggt gaatattttg gaattccttc caatgatatc aatcttttat
     2581 taccaagata ttgatttttc ttaaactctt ttgctcatat tttgttactc aattgctaaa
     2641 gaaagataca gcaaatttta gcaatatatg gttatttaat atagagtaga tttttatgat
     2701 attagaatat gtcagaagaa gtgaatatac aacaagtaaa agaagatgtg ccattgaatg
     2761 aagaagttaa tcttcagcaa atgaaagaag aagaattgat tcaaaaagaa tttgaaggtt
     2821 tgctggaaga ttatcgtaat tcaaatcatc gtcaaaaagt tgagataata acaaaagcat
     2881 tcaattttgc caaaactgca cacaaaggaa tgcgacgcca ttcaggtgaa ccttacattt
     2941 tgcatccgct ggcagtggct cgtatagttg taaaagaaat tggattaggt tctacttcaa
     3001 tctgcgctgc tttattacac gatgtggtag aagatactga ttttaccgta gatgacattc
     3061 ggaatcattt tggagacaaa atagctaaaa ttgtggaagg tctaaccaaa atttcaggag
     3121 gagtttttgg tgaaaaagcc tcaacacaag cagaaaattt ccgaaaatta ttgctcacca
     3181 tgtcggatga tattcgagta attttaataa aaattgccga tagacttcac aacatgcgga
     3241 ctttagattc gttaccaccc gccaagcaat ataaaattgc gggtgaaacc caatatattt
     3301 atgctccatt agcccatcga ttagggcttt tccgaatcaa aacggaactc gaaaatttaa
     3361 gttttaaata tgaacatccc gatatttata aatcaataga agaaaaatta agtattgacg
     3421 aagaggaaag aaaaaaattt tataaagaat tatctgttcc tatccaagaa aaactaacca
     3481 gcatgggcta tgaatttaca ctacaagaac gtgtgaaatc agtttattct atttggcata
     3541 aaatgttaac ccgaaatatt cctttcgaag aagtttatga tattctggct ttacgtatta
     3601 ttttcaagcc aaaagaagga ataagtgaaa aagatcaatg ctggatgatt tattcagcta
     3661 tcacagaaat atttcctcct catccagaac gtatccgtga ttggttaagc attcctaaag
     3721 ctaatggtta tgaagctctt catgttacgg taatgggacc aggtggacgt tgggttgaaa
     3781 tacaaatccg tacggaaaga atgcacgaga ttgctgaaaa aggtcttgcc gctcattgga
     3841 aatataaatt aaaagaaacg gaagaaacag aattagataa atggcttaaa accataaaag
     3901 aattattaga acaccctgat actgatgcca ttaattttct ggataatttc aaactcaatt
     3961 tatttgcttc tgaaatattt gtttttacgc caaaaggaga aataagaaca atagctcaag
     4021 gtgctacagc tttcgatttt gcttttttga tccactctga attgggtatg cattgtattg
     4081 gcgctaaagt taatcataaa cttgtccctc taagttataa acttcaaagt ggagatcaag
     4141 tggaaattct tacttctcaa aaacaaatgc cccaacaaga atggctaaat tatgtggtta
     4201 cagcaaaagc aaaaactgcc atcgaagaat atctaaaaaa agaagaaaga aaaatcatgt
     4261 tggaaggtca gaaattgact gaaaaaattc ttgctgaaat taacctgtct ataagtaacg
     4321 aaaatatcaa taaaattcta aaacattata acttaacaca gccaaacgaa ttttattatc
     4381 aagcagggaa aggtgtttta aatcttgatg agattaagaa catcgtattt aaacctaaaa
     4441 aacctcaaaa aacaattagc aaatatttaa aaattccttt tatttcaact tcaaatgata
     4501 aaaaggccga agttacttcc actgaagaaa aaattgattt aaaaggtact ctgaaattga
     4561 cagaagaaaa cgtaggaaaa aaatacaaat tggcagagtg ttgccatcct attcctggtg
     4621 atgatattct tggatatgtt gacgatgaca attctattat tattcataag agacaatgtc
     4681 ctgttgcagc taaattaaaa acaaattttg gtgaaagaat tgtttcggta gaatgggcaa
     4741 ctcacaaaat attatctttt aaagaaaagc ttgaaataaa aggtattgat aggaaaggaa
     4801 ttttaataga aattttaaaa gttatttcag aaaattacga atctaatatc agcgaactga
     4861 atgtaacaac aaatgatgga atttttacgg ctcaattcca actttttgtc catgacacag
     4921 aagaaataga aaacatttgt gaaaacttaa aaagaattcc tgaaattcag aaagtacaac
     4981 gcatacagga attagaaaac agcatttaaa atgattcctt aaaaatcttt cttttgaaat
     5041 ttaccagaag tagataaaat aactcgttcc ccatttttag caacataatc cttccaattg
     5101 gtatattttt tttcaggcaa aggttttccc atccgcaaat aatgacaata agctgcagca
     5161 agtccatcag ttgcatcgag ttgaggcaac atattttctg gtgatatttt caaaaaacgg
     5221 cgtaacatat cggctacttg ttcctttgat gctctcccat ttcctgtaat agccatttta
     5281 attttcaaag gagcatattc tgtaatagga atatttcgat acaaagctgc tgacattgcg
     5341 actccttgtg ctctacccaa cttcaacatt gattgtacat ttttaccata aaaaggggct
     5401 tctattgcca tgcaatcagg atgaaattca tcaattaatg aaagcgtacg tttaaaaatt
     5461 tgctgtaatt ttacataatg ctgacgatac ttttttaatt ctaacacacc cattaccatc
     5521 aactccggat cagatgctgt gacttttaat aatccgtaac ccatgattgt ggttcctgga
     5581 tcaattccca aaattatttg tgcccctttc ataaaatctt acaatttcat actcaaataa
     5641 ttattctctg caaatacata aaaatattac ctttttttaa ctctttctga tattttcaat
     5701 ccaatcaaaa atggatagca aaaatgaatc atcaaccatt atttttcctc ataagtcatt
     5761 taaaaataat tttaaacaaa agtaagcata atttctaaaa acttgcctat ctttgtactg
     5821 ttttatgtaa aacaattctt tgggggttta gctcatctgg ctagagcgcg acactggcag
     5881 tgtcgaggta accggttcaa gtccggtaat ctccactttt aaacagagtt ttttttcttc
     5941 ttcttcttct tcttcttctt ttattattat tccttccata attattatct tttcaatatt
     6001 ctttacacca ctaatctttt cacatatttt aacaactctt tactaaactt tcttaaaaaa
     6061 caattcaaac gatcataaga aaaaatttaa actattaaaa aaatattaaa atcaatttgt
     6121 tacgttttaa gagaaaaaat ttacattttg agaacttttt tctttgttag aagttatatt
     6181 tgtatattaa ataaaattat taatatctgc gggcatattc taaatattat gccaaccatt
     6241 cttgatctcg ttaaagttcc atatccagct acatatcaag gtaataaaat tcaaccgctt
     6301 gaaggtgtga gtcttagaca ggcattttta acaggtaaac aagatataca aggataccag
     6361 ttttgggaac attcgaataa gagagcagtt acaaaaggta aatggaaagc agtttctaaa
     6421 attggaacgg ataactggga gctttatgat atcttaaccc ggacaagcca gaaccaaaca
     6481 agtttttcaa agttggataa aaatatttcc ttctgcaatg tttgtcaata aggcgttaca
     6541 gaagattgtc aaaaatatta tgccaaaaaa tacatcaatt tagaagataa ggtattaaaa
     6601 aggcttcatt taaagattat tcggatcagg acatcaaaaa tattcagtac aaactaaacc
     6661 gaagacctcg agaaaaatta gattttaaat taccccaaaa tgagtttttt aaacattttt
     6721 ttaatttcgc acttgctggt tgaatctaca tagttaaaaa gggaaccatt cttaaagaca
     6781 attacgcaaa taaagaagaa atgaatgagg ctttgatggc ttttcccgta tattacatgc
     6841 tt
//
LOCUS       DAOTKS010000026         6811 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_10652_length_6811_cov_4.058911, whole genome shotgun sequence.
ACCESSION   DAOTKS010000026 DAOTKS010000000
VERSION     DAOTKS010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6811
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10652_length_6811_cov_4.058911"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="P5236_01025"
     CDS             complement(<1..246)
                     /locus_tag="P5236_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU62978.1"
                     /translation="MKKITFLMSACLTVAALQAQTEPTPTITTLWEKSAASGSLPAWF
                     LTTTNKTRGIGASNEHVFVATREGTSGKNIFVYNATTG"
     gene            complement(470..1639)
                     /locus_tag="P5236_01030"
     CDS             complement(470..1639)
                     /locus_tag="P5236_01030"
                     /EC_number="5.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008770931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HRU62979.1"
                     /translation="MNFSKLAKQYQNELLNNVIPFWLNKSQDKECGGYFTCFDGQGNI
                     FDTDKFIWLQGREVWMFSTLYNKVEKKQEWLECAIQGAEFLKKYGHDGEFNWYFSLTR
                     EGKPLIEPYNIFSYTFAAMAFGQLSLATGNQEYEQIAKATFDKILSKKENPKGKWNKV
                     HPGTRDLKNFALPMILSNLALEIEHLLDEKFLLQTLENITHEILEVFYRPELGIVVEN
                     VTLTNELSDSFDGRLVNPGHALEAMWFLMDVGERLNRPQIIEKAVEIALKMVNYGWDK
                     KHDGIFYYMDRKGYPPQQLEYDQKLWWVHIEALIAMIKGYQLTGNKECLQWFKKIHAY
                     TWSHFKDPLYPEWFGYLNRRGEVLLNLKGGKWKGCFHVPRGLYQCWKTLEKISDL"
     assembly_gap    2210..2309
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2310..3047
                     /locus_tag="P5236_01035"
     CDS             <2310..3047
                     /locus_tag="P5236_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRU62980.1"
                     /translation="VNTQTGMSYSYYFFKEEPLTEIFQQRLGLHVTIDNDSRAMAYGE
                     FMKGDVKSEKNVLFVNVSWGLGLGIIVDGKLYYGKSGFSGEFGHISAFDNEVLCHCGK
                     KGCLETQASGSYIYRKFIEKVKEGNSSILAGRIKKGESITQEDILEAVLNEDMLAIEL
                     VEEVGYTLGKHIAGLINLFNPELVIIGGVVAMTGDYLLLPIKSAVKKYSLNLVNKDTT
                     IKLSKLGDLAGVIGACMLARSKMLELI"
     gene            complement(3259..3633)
                     /locus_tag="P5236_01040"
     CDS             complement(3259..3633)
                     /locus_tag="P5236_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRU62981.1"
                     /translation="MEFIDSQPIYLQIANYVCEQILLRRWKEEERLPSVRELSALLEV
                     NPNTIMRAYDFLQGNEIIYNKRGIGYFVTKNAIEKIIDYKRKQFVGKELPVFFKNLQL
                     LDISLEEIKKLYEEYNQQQSIQ"
     gene            complement(3633..4388)
                     /locus_tag="P5236_01045"
     CDS             complement(3633..4388)
                     /locus_tag="P5236_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU62982.1"
                     /translation="MYQTFSLKRIDLLLKIYFVENQNYEIYLWSILTLIFMFFKGEGT
                     AQLITLIVGIVYATRFFKIFAYSSNGTHYLMIPATQVEKLITVFLLNTFYFFLMIIIT
                     SVIGNFIGTILRNLIYGISTPFSFQFFFPYSKLNSDFDITYLLKNFGVFATSQAIFTL
                     GSLYFKRNSIAKTLLTLMMISILFGMIEYTVFKVTMGSLLSALNQYDGFQMAWDEIIF
                     PKGMIFVWKIIGYLLIPFCWIVSYFRLTEKEIK"
     gene            complement(4405..5262)
                     /locus_tag="P5236_01050"
     CDS             complement(4405..5262)
                     /locus_tag="P5236_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRU62983.1"
                     /translation="MIDIKNLEFNYSHSSHLFKNLNLSLFAGHIHGLLGKNGEGKSTL
                     LKMISGLVFPLKGEISVMNVEPRKRHPEMLQEIFFLPEELPPVNLSMENYEKVYAPFY
                     PHFSSEQFHQYLTEFEMESAKTQMNQLSYGQKKKFFIAFGLAANTSLVLLDEPTNGLD
                     IPSKAQFRKMAALAIEENRCIIISTHQVRDLDSLIDSIVIMNGHEIVFNEFTENITKK
                     LLFKVSNQKEEKEEVLYAEENLRGTYQVVENKTGEESRLDIELLFNAVLQNQQRIKEI
                     FSNQPNNFK"
     gene            complement(5659..6708)
                     /locus_tag="P5236_01055"
     CDS             complement(5659..6708)
                     /locus_tag="P5236_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HRU62984.1"
                     /translation="MKWDTVKITNQHGEMIEAIAPIIISASRATDIPAFYSDWLIQRI
                     NEGYLKWNNPFNGVSSYVAFSKARFFVFWSKNPKPLLKYLDFFEEKGFNYYFQFTLND
                     YDKEKLEPRVPNVQQRIETFQQLSERIGKEKVIWRFDPLILTDKIGIDELLRKIENIG
                     NQLKNHTQKLVFSFADIKIYKKVQNNLRNSSIHYQEFNERTMNEFAAGLQALNKNWNF
                     ELATCAEQISLEKYGIKHNKCIDDDLIIKLFYQDKKLMDFLAVKIIPADIFHPTPTFV
                     KTKDNKDKGQRKFCGCIVSKDIGEYNTCPHLCEYCYANSSKQIALENWELHKRHPYDE
                     LLFRSIKSPKLSQII"
BASE COUNT         2301 a          981 c         1028 g         2401 t
ORIGIN      
        1 acctgtcgta gcattgtata caaaaatatt tttaccgctc gttccttctc tggtagccac
       61 aaaaacgtgt tcattagagg ctcctatacc acgtgtttta tttgtagtag ttaagaacca
      121 tgcaggtaaa gaacctgaag ctgcagattt ttcccacaga gtagtaatgg tgggagtggg
      181 ctcagtttgg gcttgtaatg ctgctacagt aagacaagca ctcattaaaa aagtaatttt
      241 tttcatgatt tttgtttttt agattgtgaa tgaataaaat taattaaaaa aaatgtttat
      301 ttgcatgtag aagaaaatta ttaagaaaat gtatttacaa agaaataaaa aattaattat
      361 aaagtcaatg gttttttaaa aaaattttta taaataattt gtgtacatga aaattttttt
      421 attacctttt tattaagaaa aattttaaca tataatccct ttattttttt tataaatctg
      481 atatcttttc taaggttttc cagcattgat aaagtcctct gggtacgtgg aaacaacctt
      541 tccattttcc tcctttcaga ttcaaaagca cttctccacg acggttaaga taaccgaacc
      601 attccggata gagaggatct ttaaagtgtg accatgtgta agcatggatt tttttaaacc
      661 attgtaagca ttctttgttt ccagtcaatt gatatccttt aatcatggca atcaacgctt
      721 caatgtgaac ccaccataac ttttgatcat attctaattg ttggggtgga tatcctttac
      781 gatccatgta gtaaaaaata ccgtcatgtt ttttatccca gccataatta accattttaa
      841 gtgctatttc tacagctttt tcgatgattt ggggacgatt taaccgttca ccgacatcca
      901 taagaaacca catggcttct aaagcatgac cggggtttac caatctgcca tcaaatgagt
      961 ctgataattc attagttaac gttacatttt cgactacaat tcctaattct ggtcgataaa
     1021 atacttctaa aatttcatga gtaatatttt caagagtttg taagaggaat ttttcatcca
     1081 ataagtgctc tatttccagg gcaagattag aaagaatcat tggaagagca aagtttttta
     1141 aatctcgagt tccgggatga actttattcc atttcccttt tggattctct ttttttgata
     1201 atattttatc gaaagtagct tttgcaattt gctcgtattc ttggttgcca gtagccaaac
     1261 ttaattgacc gaaagccata gcagcaaatg tataggagaa aatattatat ggttcaatta
     1321 aaggttttcc ttcccgtgtt aatgaaaaat accaattaaa ttcaccatca tgtccatatt
     1381 tctttaaaaa ctcagctcct tgaatggcac attcgagcca ttcttgtttc ttttcaactt
     1441 tattatataa agtagaaaac atccaaactt ctctcccttg caaccaaata aatttatcag
     1501 tatcgaaaat attgccttgt ccatcgaaac aagtgaaata accaccacat tctttgtctt
     1561 gcgacttatt aagccaaaat ggaataacat tatttagtaa ttcattttgg tattgcttcg
     1621 ctaatttact aaaattcatc attttatttg attttaaatt atttattata ataaaattga
     1681 aattttaaat ttcttttttg agaataatct tttcttgcct tttattttta taatttactt
     1741 ttcttgagaa tacagaaaaa tttcaaatgt aagttttatt ttaatgaaat aatgttctaa
     1801 aaaataatta ttgaatttcg cttcaaatat aaaatatatt taataataat ccaaataaaa
     1861 ataaaaatat tttataaata ttttttgaaa aatattttat aataaaaata aagaaagatt
     1921 tttgaatttt attattaaaa aatttatttg ttaatagaag atttcaaaaa aagaattaaa
     1981 tttgtaaatg cataaaaatt ctttttttaa aaagaaagaa gcagttcaat ttgagaataa
     2041 ataagttttt taattgtgac tttttaagaa aaactaaatg tgaaaaatta ataataaaga
     2101 atatgaggta caaacttatc gacgaaatag agttaggttc taaaaatgct ctattaaaaa
     2161 taaatatctt acaatattat attaataatg gtgaaaatag tttggctgan nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng taaatacgca gacgggcatg agttatagtt
     2341 attacttttt taaggaagag ccattgacag aaatttttca gcagcgatta ggtttacacg
     2401 ttactattga taatgattct cgtgctatgg cttatggaga atttatgaaa ggggacgtaa
     2461 aaagtgaaaa aaatgtgttg tttgtcaatg taagttgggg tttgggactc ggtattattg
     2521 tggacggcaa actttattat ggaaaatcgg gcttctctgg tgaatttgga cacattagtg
     2581 cttttgataa tgaagtgctt tgtcactgtg gcaagaaagg ctgtcttgaa actcaagctt
     2641 caggatcgta tatttataga aaatttatag aaaaagtgaa ggaaggaaat tcttctattc
     2701 ttgcaggaag aattaaaaaa ggagagagca tcacccaaga agatatattg gaagctgttt
     2761 taaatgaaga tatgctggca attgaattag tggaagaagt aggatataca ttagggaaac
     2821 acattgctgg tttaattaat ctttttaatc ctgaattagt tattattgga ggagtggttg
     2881 ctatgactgg tgattatttg ttattgccca tcaaaagcgc cgtaaaaaaa tattctttaa
     2941 atctggtcaa taaagatacc accatcaaat taagcaagtt aggtgatctt gccggcgtga
     3001 taggagcttg tatgctggca cgaagcaaaa tgttagaatt aatttaatcg atgtattacg
     3061 aaaacttata ggtatttata aattttctat agaatcgctt ggttgaacca tttcataatt
     3121 ttcttcattg cttggttgca aaaaaacatt tatcctattt tttaatatta tgttactgat
     3181 aatcaaaaga ataattataa aaataaatag tccaattaat aatttattgc ttgttttcat
     3241 tgttgattat ttcttagttt attgaataga ttgttgctga ttatattctt cataaagttt
     3301 tttaatttct tcaaggctga tatcaagcaa ttgcaaattt ttaaaaaaaa ctggcaattc
     3361 ttttccaaca aattgttttc gcttgtaatc tataattttt tcgatagcat tttttgtaac
     3421 aaaatatcct atacctcgtt tgttgtaaat aatttcattt ccttgcaaaa agtcataagc
     3481 tcgcattatg gtgttaggat tcacctccaa taaagcactt aattctcgta ctgagggcaa
     3541 tcgttcttct tctttccaac gacggagcaa gatttgttcg caaacataat ttgcaatttg
     3601 cagataaatc ggctgtgaat caataaattc cattatttta tttctttttc tgttagacga
     3661 aaatagctaa ctatccagca gaaaggaata agtaaatagc ctataatttt ccaaacgaat
     3721 atcattccct ttgggaaaat aatttcatcc caagccattt gaaaaccgtc atactgattc
     3781 aatgctgaaa gtaaagaacc cattgtcact ttaaaaaccg tgtattcaat cattccaaat
     3841 agaatactta tcatcataag cgttaacagg gtttttgcta ttgaatttcg tttgaaatag
     3901 agggaaccaa gtgtaaagat agcttgtgaa gtagcaaaaa ctccaaaatt tttcagcaaa
     3961 taagttatgt cgaaatcaga atttagttta gaatagggga agaaaaattg aaaagaaaaa
     4021 ggagtactta ttccataaat caaatttcgc agaatagttc cgataaaatt tcctataact
     4081 gaggtaataa ttatcattaa aaagaaataa aaagtattca gcagaaatac agtgattaat
     4141 ttttcaacct gagtggctgg tatcattaaa taatgtgtac cattggaaga ataggcaaaa
     4201 attttgaaaa aacgcgtggc atatactatg ccaacaataa gcgtaatcag ttgtgcagtt
     4261 ccttcacctt tgaaaaacat gaaaattaac gtcaaaatac tccaaagata aatttcatag
     4321 ttttgatttt caacaaaata tatttttaga agtaaatcaa ttcttttgag gctgaatgtt
     4381 tgatacatat tttttatttt aatcttattt aaaattgttc ggttgatttg aaaaaatttc
     4441 ttttattcgt tgttggtttt gcaaaacagc attaaataaa agttcaatat ctaacctgct
     4501 ttcttcacct gttttgtttt caacaacctg ataagtacca cgaagatttt cttccgcgta
     4561 aagtacttcc tccttttctt ctttttgatt gctgactttg aacaagagtt ttttggtaat
     4621 attttcagtg aattcattga aaacaatttc gtgtccattc attataacaa tgctgtcaat
     4681 caagctgtct aaatccctta cttgatgcgt ggaaatgata atacaacgat tttcttcaat
     4741 ggccagagct gccatttttc tgaattgagc ttttgaaggg atatccaatc cattggtggg
     4801 ttcatcaagc aaaacaagac tcgtattggc tgccaaacca aaagcgataa agaatttttt
     4861 cttttgccca taagaaagct ggttcatctg tgtttttgct gattccattt caaattcagt
     4921 taaatattga tgaaattgtt ccgagctaaa atgaggataa aaaggagcat agactttttc
     4981 ataattctcc atgctcaaat taacaggtgg caattcttct ggaaggaaaa atatttcctg
     5041 caacatttct ggatggcgtt tccgtggttc aacattcatt acgctgattt cacctttcaa
     5101 tggaaaaacc agtccagata tcattttcaa caaggtactt ttgccttcgc cgtttttccc
     5161 caataaaccg tgaatatgtc cagcgaacaa tgataaattt aagttcttaa acaggtgaga
     5221 agaatggctg taattgaatt ccagattttt aatatcaatc ataagaatat tttaattgat
     5281 tagtgtgtta gtataatagt acactacaaa tgtaatatca aaatttatta ttatgcaaaa
     5341 atatttagtt aaatcttgtt aatttgcaaa atatttttgt gattggttga ttatgagcat
     5401 gtaaaattta atagataacg aaaaaatgtg agaatagtag gaataacatg tttcattttt
     5461 ccatactata ataataatta tggagtggaa ataaatttat tgaaaggaaa atccgcttta
     5521 agatgaaaaa atcaaatttt tccctggtat aaaaatttta cttaaaggca aaccaaaata
     5581 ttaaacgcat ttttgaatgt ttttattccc tcccccacaa caattttatt acactaaaaa
     5641 taattttttc tcttaacatc atataatctg cgaaagctta ggagatttaa ttgaacgaaa
     5701 caataactca tcataaggat ggcgtttatg caattcccaa ttttctaaag ctatttgttt
     5761 ggaagagttt gcatagcaat attcacacaa atgtggacag gtattatact cgccaatgtc
     5821 tttgctgaca atgcatccac aaaattttct ttgtcctttg tctttattat ctttggtttt
     5881 tacaaaggtt ggagtgggat gaaatatatc tgctggaatg atttttacgg caagaaaatc
     5941 catcaacttt ttgtcctgat agaaaagttt aatgatcaaa tcatcatcaa tacatttatt
     6001 atgctttata ccatattttt ccaacgaaat ttgttcggca catgttgcta attcgaaatt
     6061 ccaattttta ttcaaagctt ggagccctgc tgcaaattca ttcatggttc gttcgttaaa
     6121 ctcttgataa tgaatagaac tgtttcgcag attgttttga acttttttgt agattttaat
     6181 atcagcaaaa ctgaaaacca gtttttgagt atgatttttt aattgatttc caatattttc
     6241 tatttttctc aataattcat caattccaat cttatcagtt aaaatcaggg gatcgaagcg
     6301 ccaaatcact ttttctttgc caattcgttc ggataattgc tgaaaagttt ctattctttg
     6361 ttggacattg gggacacgag gttctaattt ttctttgtca taatcattca atgtgaattg
     6421 aaaataataa ttaaatcctt tttcctcaaa gaaatctaaa tatttcaata gcggttttgg
     6481 attttttgac cagaacacaa aaaatcgtgc ctttgagaaa gcaacataag aagaaacacc
     6541 attaaaagga ttgttccatt taagataacc ttcattgatc ctttggatta accaatcgga
     6601 ataaaaagca ggaatatccg tagcacggct ggctgaaatg ataatggggg ctatcgcttc
     6661 tatcatttca ccatgttggt ttgttatttt aactgtatcc cacttcatta attttcaatt
     6721 gattaaaaaa acaagaataa agatactaaa aaacttacta catttgacaa atcatgagta
     6781 aaatattttc aaattggttt taactgagaa g
//
LOCUS       DAOTKS010000027         6778 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_10704_length_6778_cov_5.680797, whole genome shotgun sequence.
ACCESSION   DAOTKS010000027 DAOTKS010000000
VERSION     DAOTKS010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6778
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10704_length_6778_cov_5.680797"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="P5236_01060"
     CDS             <1..575
                     /locus_tag="P5236_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HRU62985.1"
                     /translation="EIHGDMALAGNQHVPFASVSNKAAQISITLMSPSKIFNIPSLQA
                     AYYIIPNEEIRNLFKDFLNQLRITDGNIFAYIATIAAYTQGTEWRRQMLQYVNKNVEY
                     VIHFLKEELPLIEPMKPEASFLIWLNCKKMGMTDEESFQFFIKKAGLGLNKGKIFGPG
                     GEQHFRLNVACPRNVLQQAMKQLKSAFESF"
     gene            587..1249
                     /gene="ung"
                     /locus_tag="P5236_01065"
     CDS             587..1249
                     /gene="ung"
                     /locus_tag="P5236_01065"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRU62986.1"
                     /translation="MNVKIEPTWQTVLQPEFDKDYFIRLTDFVKNEYKTRQIFPPGSL
                     IFNAFNLCPYDKVKVVILGQDPYPNEGQAHGLCFSVNENVEFPPSLMNIFKEIQAEFG
                     KPIPKNGNLTRWAEQGVLLLNAILTVQAHLPTSHQGKGWEIFTDAVIRKLATEKEHLV
                     FMLWGNYAQQKGAFIDPNRHLVLKSSHPSPLSANRGFFGNQHFMLANQYLKAHGEKEI
                     DW"
     gene            1345..2094
                     /locus_tag="P5236_01070"
     CDS             1345..2094
                     /locus_tag="P5236_01070"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HRU62987.1"
                     /translation="MTLQEQFEKQGNFLFKYRSYLPLIIIVAGLLVYIFPLLKNPNPS
                     FASYTILVFISLAISFIGFAIRVYTVGHTPANTSGRNTAEQIADELNTTGIYSMVRHP
                     LYLGNFFMWLGAALLTTNPWFVVIFVLVYWLYYERIMYAEEQFLQRKFGEAFLRWAEK
                     TPTFIPNFHRFVSSRYPFSWKKAIKKEKNGLAAIFILLFIFYNIRYSFKNHHFSIDLN
                     WIFWVTLLTILLYLVLKFLKKETHVLDETGR"
     gene            complement(2162..2740)
                     /locus_tag="P5236_01075"
     CDS             complement(2162..2740)
                     /locus_tag="P5236_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HRU62988.1"
                     /translation="MEKSLKGTKTEKNLLKAFAGESQARNRYEFFASVAKKEGYEQIA
                     AIFMETASQEKEHAKRFFKFLEGGMVEITASYPAGVIGNTKQNLKAAAEGEYDEWSNL
                     YPTFAQVAEEEGFSEIAIAFKMVAKVEVEHERRYLKLLQNISEDKVFKKDGKVWWKCR
                     NCGYIYESAEALETCPVCLHPKAFMELWQENY"
     gene            complement(2815..2955)
                     /locus_tag="P5236_01080"
     CDS             complement(2815..2955)
                     /locus_tag="P5236_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006949761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HRU62989.1"
                     /translation="MNKKFKCLVCGYIYDEAQEKVKFEDLPDDWVCPLCGTEKSEFIE
                     IK"
     gene            complement(3058..3492)
                     /locus_tag="P5236_01085"
                     /pseudo
     CDS             complement(3058..3492)
                     /locus_tag="P5236_01085"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
     gene            complement(3676..4635)
                     /locus_tag="P5236_01090"
     CDS             complement(3676..4635)
                     /locus_tag="P5236_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRU62990.1"
                     /translation="MKKTKVKHPFWVMVSKEISDQVTSWRFIVMILLIVLTCMGSLYT
                     ALGQFTKSSASVGSDSFFFLKLFTLSDGTLPPFIIFISFLAPLLGISLGFDAINSEYN
                     RGTLSRILSQPIYRDYIINAKFMAALMVVSFLFFTLGLLVIGCGLIALGIPPSSDELF
                     RILFYLIISIVYVAFWLNLSILFSVTFRQPATSALSGIAVWLFFSVFYNMIIELLAKG
                     LAPRGMVTEQQIVNFQNGILNLMRLNPSQLFSDANITLLMPSVRSLGALTMSQVEGAL
                     PTPLPLGQSLLMVWPQVTGLIAGTILLFAISYYLFMRQEIRSR"
     gene            complement(4703..5458)
                     /locus_tag="P5236_01095"
     CDS             complement(4703..5458)
                     /locus_tag="P5236_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRU62991.1"
                     /translation="MSEIMVELVNLTKKYKSFTAVDQINLSVKKGEIFGFLGPNGAGK
                     STTILMMLGLTEPTEGKVEVCGINPTRYPIEVKRKVGYLPEDVGFYDNRTGLYNLLYT
                     AMLNRIPENEARNKALELFQLVGLEGVENKKTGQYSRGMRQRLGLADVLIKNPEMIIL
                     DEPTSGIDPQGVKSFLDLILQLRNEQGLTILFSSHNLHQVEQVCDAVGIFSKGKIIAS
                     GNIPDLSRQVFNDETHSLEDVYNQFFEGGNQDE"
     gene            complement(5461..6627)
                     /locus_tag="P5236_01100"
     CDS             complement(5461..6627)
                     /locus_tag="P5236_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HRU62992.1"
                     /translation="MGGILMKLWKKSFNQHLILLCFFIFFSFLTSFASKNVLVYTPYT
                     QVFVSPGESVDYTIDVINNGESIFNQEITINGIPRNWNYILRLDGYKVGRVAVLPKEK
                     KSLSLKVEVPFQVNKGTYAFRVAAGDSASLTLRITVTEKGSSETEFTTDQANMQGNAT
                     SNFSFRTTLKNRTAEKQHYALIADAPRGWTVTFNADYKQVTSVEMEPNTTKDITIDIK
                     PTAKVKSGTYKIPVRAMTGSTSADLELEVVITGNYAIELTTPTGLVSTNITAGKEKKV
                     TLVVKNTGSADLSGIEMKATTPSKWEVTFEPSKIDKLLPGEQATVNATLHADKKAIPG
                     DYIVNINASTPEATADISFRVMVKTSMVWGWIGIIIILAALGGVFYLFRKFGRR"
BASE COUNT         2174 a         1160 c         1099 g         2345 t
ORIGIN      
        1 atgaaattca tggcgatatg gctttggcag ggaatcaaca tgttcctttt gccagcgtgt
       61 ccaataaagc tgcacaaatc agcattacgc ttatgtcgcc aagcaaaatt tttaacatac
      121 cttcattgca agcggcttat tatattattc caaatgaaga aattagaaat ttattcaaag
      181 attttctgaa tcagttaaga ataaccgatg gaaacatttt tgcttacatt gcgaccattg
      241 ctgcttacac acaaggaaca gaatggcgac ggcaaatgtt gcaatatgtc aataaaaatg
      301 tggaatatgt tattcatttt ttaaaggaag agcttccact aattgagcct atgaaaccag
      361 aagcttcttt cctgatctgg ctgaattgta aaaaaatggg aatgactgat gaggaatcat
      421 ttcaattttt tataaagaaa gctggtttgg gacttaataa ggggaaaatt tttggaccgg
      481 gtggcgagca acatttcagg ttgaatgttg cttgtccacg aaatgtgttg cagcaagcaa
      541 tgaaacaatt aaaaagtgcg tttgaatcat tttaattttt aaagatatga atgtaaaaat
      601 agaacctacg tggcaaacag ttttacagcc agaatttgac aaggattatt ttattcggtt
      661 aactgatttt gtaaaaaatg aatataaaac gagacagatt tttccacctg gttctttgat
      721 attcaatgct tttaatcttt gtccttatga taaggtaaaa gtagttattc ttggtcaaga
      781 tccttacccc aacgaagggc aggctcatgg tttatgtttt tccgtgaatg aaaatgtaga
      841 atttccacct tctttgatga atatttttaa ggaaattcaa gcagaatttg gtaaaccgat
      901 tccaaaaaat ggaaatttaa ctcgttgggc tgagcaaggc gttttattac tcaatgctat
      961 tttaactgtg caagctcatt tgccaacttc tcatcaaggg aaaggttggg aaatctttac
     1021 tgatgcggtt attcggaaat tagctactga aaaggaacat ctcgttttta tgttatgggg
     1081 aaattatgct cagcaaaaag gtgcatttat tgatccaaat cgtcatctgg ttttaaaatc
     1141 gagccatcct tcacctcttt ctgccaatag aggtttcttt ggtaatcaac attttatgct
     1201 tgccaatcag tatctcaaag ctcatggaga aaaagaaatc gattggtaaa aaattcttat
     1261 atttttcaaa aaataaaatg ttcagttgta cgtttttaat aagcatttga ctttaaagtg
     1321 tttaactttt aatttattta ttcaatgact ttacaagagc aatttgaaaa acaagggaat
     1381 tttttgttca aatatcgcag ctatttgcct ttgataatta tagttgcagg tttattggtt
     1441 tatatttttc ccttattgaa aaatccaaat ccttcttttg catcttatac aattttagta
     1501 tttataagtt tggctatttc atttattggt tttgctattc gagtttatac agtgggacat
     1561 actccggcaa atacttctgg aagaaataca gccgaacaaa ttgcagatga attaaatacc
     1621 actgggattt attcaatggt tcgacatcca ctttatttag gaaatttttt tatgtggctg
     1681 ggcgcagctt tattgactac aaatccttgg ttcgttgtaa tatttgtatt ggtttattgg
     1741 ctttattatg aaagaattat gtatgctgaa gaacaattct tacaacgcaa atttggtgaa
     1801 gcatttcttc gttgggcaga aaaaacgcct acttttattc ccaattttca tagatttgtt
     1861 tcatctcgtt atccttttag ctggaagaaa gctataaaaa aagaaaaaaa tggtttggct
     1921 gctattttta ttttactttt tattttttat aatatcagat attccttcaa gaatcatcat
     1981 ttttcaatag atttgaattg gattttttgg gtaacacttt tgaccatttt actttatctg
     2041 gttttaaaat ttctgaaaaa ggaaacgcac gttttagatg aaacgggtag ataaaattga
     2101 atctctctga aaaaattttc agagaggttc aataaatttt tgagaaataa tgttatgata
     2161 atcaataatt ttcctgccat aactccatga atgcttttgg atgcagacaa acaggacaag
     2221 tttccagagc ttctgctgat tcatagatgt agccacaatt tctgcatttc caccaaactt
     2281 tgccatcttt tttaaagact ttgtcttcgg aaatgttctg caaaagtttg agataacggc
     2341 gttcatgttc tacttctact tttgctacca ttttaaaagc aattgcaatt tcagaaaatc
     2401 cttcttcttc agcaacttga gcaaaagttg gatacaagtt tgaccattca tcatattctc
     2461 cttcagcagc agctttcaag ttttgcttgg tattaccaat tacgccagcc ggataagaag
     2521 cggtgatttc aaccattccg ccttccagaa acttaaagaa acgtttggcg tgttcttttt
     2581 cttgactggc agtttccata aaaatagctg caatttgttc gtaaccttct tttttggcca
     2641 cgctggcaaa gaattcataa cgattgcgag cttgtgattc tcctgcaaag gctttcagta
     2701 agtttttttc agtttttgta cctttcaatg atttttccat attttttata attaaattgt
     2761 attacaatag aaattgatta aacatgattt atttgataat atttttgact tgttttattt
     2821 tatttcaata aattcagatt tttctgtgcc acaaagtggg catacccaat cgtcaggtaa
     2881 atcttcaaat ttaacttttt cttgggcttc atcataaata taaccacaaa cgagacattt
     2941 aaattttttg ttcattgaat ttattttaaa agttgataaa ttagttggat tgatataagt
     3001 aggagcattt ttgggtgata ctcctttttt tatttctcgg taataagcat aagttaacgg
     3061 tggctgagtg tcatctatta tttgtgcatc aactacatca ccaataaaca gcaaatgagt
     3121 acccacatca aaagtttgta tcactttaca ttctaaaaaa gcaatgctat catttaatac
     3181 gatgggaaca cctgtttgtc cataaataat attatttcca ctcattttat cggtttctct
     3241 accacttttg taaccaaatt tgccgataat ttcactggaa gcatcttgtc cgagaacaga
     3301 gacagagaaa actttatatt gattaataaa ttcacaggta taattgtctt tattgcagca
     3361 aactgtaaat cgcggaggtt cagaagttac ctgaaatact gtgttggaaa taaaaccatt
     3421 gcctttttct ttatttcctg aacttacaat atacaaacca taagaaattt taaaaaatgt
     3481 cgttacgttc attgaagaaa attcgagttt ttatttttat tttttgaatt attctctttt
     3541 aaaattctaa caaacaaatt taaagattaa tttcatttga acaatattct cataaatatt
     3601 tttcacttgg aaataaaaag gatgcttttt tcaaaacatc ctctcaataa aatgggctca
     3661 tttgtttgta tttaattatc ttgatctgat ttcttgacgc ataaatagat aataacttat
     3721 tgcaaacaaa agaatagttc ctgcaataag tccggttact tgcggccaaa ccatgagcaa
     3781 actttgacca agaggcaggg gtgtaggaag agcaccctcc acttgtgaca ttgttaatgc
     3841 tcccagactt cttaccgatg gcatgagaag agtaatattg gcatcactaa aaagctggct
     3901 gggatttaat ctcattaaat ttaaaattcc attttgaaag tttacgattt gttgttctgt
     3961 aaccattcct cttggagcca agcctttggc aagcaactca ataatcatat tataaaaaac
     4021 gctgaaaaaa agccacactg caatacctga taaagcagaa gtagctggtt gcctaaaagt
     4081 tactgaaaaa agaatagata aattaagcca gaaggcgaca taaactatac taattatcaa
     4141 gtaaaataat attcgaaaca attcatcgga ggaaggagga attcctaaag caatcaatcc
     4201 acaaccaatt accagcagtc ccagcgtaaa gaaaagaaaa cttactacca tcaatgcagc
     4261 cataaatttt gcattgatga tgtaatctct gtaaataggc tggcttaaga ttctacttaa
     4321 agttcctctg ttgtattctg aattaatagc atcaaatcct aaacttatcc caagcaaagg
     4381 cgccagaaaa ctgataaaaa tgataaaagg tggcaaagtg ccatcagata aagtaaataa
     4441 ttttagaaaa aagaaactgt cgctcccaac gctggcagaa gattttgtaa actgccccaa
     4501 agctgtataa agtgacccca tgcaggttaa cacaattaat aaaatcatta caataaatcg
     4561 ccagctcgtt acttgatctg atatttcctt tgaaaccatt acccagaaag gatgttttac
     4621 tttcgttttt ttcataactg acaagtttgt ttcaaaagta ggttgaaaat aaccagagag
     4681 tttttctgca gaagaaaaag cattattcat cttgattccc tccttcaaaa aattgattgt
     4741 acacatcttc caagctgtga gtttcatcat tgaaaacttg cctggacaaa tccgggatat
     4801 tgcctgaagc aattattttt ccttttgaaa aaattccaac tgcatcacaa acttgttcca
     4861 cctgatgaag attatgagaa gaaaaaagaa tagttaatcc ttgctcgtta cgtaattgca
     4921 aaattaagtc caggaatgat tttacacctt gcgggtcaat acctgaagtt ggttcatcca
     4981 gaataatcat ttctggattt tttattagca catctgccaa gccaagtcgc tgacgcattc
     5041 cacgcgaata ttgccccgtt tttttatttt ccacaccttc caagccaact aattgaaaaa
     5101 gttctaatgc cttatttcga gcctcatttt cgggaattcg attaagcata gctgtgtaga
     5161 ggaggttata cagtcctgtt ctgttatcgt aaaatcccac atcttcgggt aaatatccga
     5221 cttttcgttt tacttcgata ggataacgtg tcgggttgat accacaaact tcaacttttc
     5281 cttcagttgg ttcggttaat cctaacatca tcaagatggt ggtagatttt cctgccccat
     5341 taggacctaa aaaaccaaat atttcccctt tttttaccga taaatttatc tgatcaacag
     5401 ccgtaaaaga tttatatttt tttgtcaagt taaccaattc aaccattatt tcggacatag
     5461 ttacctcctt ccgaattttc tgaataaata aaatacgcca cctaaagctg ctaatataat
     5521 gatgattcct atccaccccc acaccataga agttttaacc attactctaa atgatatatc
     5581 agcagtagct tcaggagtac tggcattaat attaacaata taatcacccg gaatagcttt
     5641 tttatctgca tgtagagtgg cattgacagt agcttgttca ccaggaagta atttgtctat
     5701 ttttgatggt tcaaaagtga cttcccattt agatggagtt gttgctttca tttcaattcc
     5761 agataaatca gcggaccctg tatttttaac aacaagtgtt actttctttt cttttccagc
     5821 cgttatgttt gtgcttacca aacctgttgg agtagtgagt tctatggcat aatttcctgt
     5881 aattaccact tccagttcta agtcagccga agtagaacct gtcattgcac gcactggtat
     5941 tttgtaagtt cctgatttta cttttgcagt aggttttata tcaatggtaa tatctttggt
     6001 agtattgggt tccatttcaa cagaggtaac ttgcttataa tcagcattaa aagtaacagt
     6061 ccaacctctt ggggcgtcgg ctatcaaggc ataatgttgt ttttcggctg tcctgttttt
     6121 gagcgttgtt ctaaatgaaa aatttgatgt tgcatttccc tgcatatttg cttgatccgt
     6181 ggtaaattct gtctcgcttg aacctttttc agtgaccgta atccttaagg ttaaagaagc
     6241 agaatcacct gctgcaactc taaaagcata ggtcccttta tttacctgaa aaggaacttc
     6301 cacttttaag gaaagactct ttttttcttt cggaagaaca gcaactcttc ctactttata
     6361 tccgtccagt ctcaatatgt agttccaatt ccttggaata ccattaatag ttatttcttg
     6421 attaaaaatg gattcaccat tgttaataac atcaattgta taatcaactg attctcctgg
     6481 cgaaacaaat acctgtgtat aaggagtata cactaaaaca tttttagaag caaaggaagt
     6541 taaaaatgaa aagaaaataa aaaaacataa aagaatcaag tgttgattaa aagatttttt
     6601 ccataatttc ataagaattc ctcccattct ttaagtatgt ttatgtaata caattgcaag
     6661 tttagtaaat tattttattt ataaatgtta atttttttaa aaaattaaaa tcgtatttta
     6721 aagcaggata agttgtattt cttttttatc aattattcta aattaaattg ataatctg
//
LOCUS       DAOTKS010000028         6748 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_10751_length_6748_cov_4.718811, whole genome shotgun sequence.
ACCESSION   DAOTKS010000028 DAOTKS010000000
VERSION     DAOTKS010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6748
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10751_length_6748_cov_4.718811"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(913..1083)
                     /locus_tag="P5236_01105"
     CDS             complement(913..1083)
                     /locus_tag="P5236_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU62993.1"
                     /translation="MKKLVLLFAVVFAFVACGKKATQESTETDTTSVVAPVDTIVESI
                     DTTTVDTTVAPM"
     gene            complement(1294..1464)
                     /locus_tag="P5236_01110"
     CDS             complement(1294..1464)
                     /locus_tag="P5236_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU62994.1"
                     /translation="MKKLVLLFAVVFAFVACGKKATEEKAESEDSIVIVDTIESQIDT
                     TNVIDTMATPFE"
     gene            complement(1814..4258)
                     /locus_tag="P5236_01115"
     CDS             complement(1814..4258)
                     /locus_tag="P5236_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HRU62995.1"
                     /translation="MVYPQNFEQKIDFGNIRQLLKEKSISTLGKEKIDSIHFSSDFYK
                     IIQQLDQTNEMLQILTTETNELPLSNIYDIRPSLHKIHVEGLYLTENELYCLRTNLEN
                     AQLLTTYFSKLNSTFYPQLSRLSKTVKSFSFLIDQINKILTKYGDIKDNASPQLEKIR
                     KNILTTQNNISRTLNEILQRAFSNGYVEKDTLPTLREGRLVIPVSQAFKRKINGIVHD
                     ESASGKTVYIEPAEVIELNNSIRELEGEERREIIRILTDITNYIRPFIPDLLELQNFL
                     GEIDFLHSKALFAIDIKAIRPNVENKCIIQWNKASHPLLYLSLQKQGKNIVPLDIVLN
                     ENQRILIISGPNAGGKSVCLKTVGLLQYMLQCGLLIPLDESSQIGIFENLFIDIGDEQ
                     SIENDLSTYSSHLLNMKFFIQHSNEKTLVMIDEFGSGTEPQIGGAIAEAVLERINQQK
                     AFGVITTHYTNLKHFAENTEGVVNGAMLFDRGRLQPLFQLQIGFPGSSFAIEIARKIG
                     LPDDIIEEAAQKVGKEHFDYEKHLHEIIRDKRYWENKRKKIRQREKYLEKIIEQYETT
                     LNNIEKKQKEIIQATKAEAIQLLNEANAKIENTIRQIKEAQAEKEKTKEIRKELETFK
                     KEKLEPEILPKPIEPSIQLKRKKSKKVASENLSKSTFQVGNYVKIKGHSLVGRIIEIS
                     EEQAIIAFGQMKSTIELSKLEFINRTQTKKEINKIQQSIELKNEEIKNRKLNFKTEID
                     VRGMRGEEAVQAVSYFIDDAIMVGANSVRILHGTGSGVLRQLIRQYLATVPQIKNFHD
                     EHIELGGSGITVVNFE"
     gene            complement(4270..5070)
                     /gene="mazG"
                     /locus_tag="P5236_01120"
     CDS             complement(4270..5070)
                     /gene="mazG"
                     /locus_tag="P5236_01120"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HRU62996.1"
                     /translation="MEDKKRTLDEIKEEFGRLVEIMNELRQKCPWDKEQTFDSLRVQT
                     IEETYELSDAIMKKDMEEIKKELGDLLLHIIFYAQMANENNDFDIYEVCKALNEKLIY
                     RHPHVFSNVKSDSTETVVQNWEKLKLKEKNGNKTVLGGVPDSLPALIKAHRIQDKARY
                     VGFDWEEKEQVWEKVKEEFLELQQEIKSLDKDKIEEEFGDLLFSLINAGRLYDINPEN
                     ALERTNRKFINRFNYLESKTISQGKDLKQMTLQEMDEIWNEAKKLESK"
     gene            complement(5302..6498)
                     /locus_tag="P5236_01125"
     CDS             complement(5302..6498)
                     /locus_tag="P5236_01125"
                     /inference="COORDINATES: protein motif:HMM:NF037429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VLRF1 family aeRF1-type release factor"
                     /protein_id="HRU62997.1"
                     /translation="MISKNDIQRFKTLVSSINEPVLSVYCNVNPAIPENVGRAWYDRL
                     KNTLKSMNELNIKLKKGKSFYEELIIFLDQIRTEARTLVLFAWINEKGELAVEHFELQ
                     VDLPVVDLRSGRVEAHFGKPYLTPILYAFDEFRRVGVLHIYGNKWRFYEVFMNEVKEV
                     TDVFAAITPNEWKEFNEYSQIIESGVLEDKMFNAKMKFKDKIKAKQQTFSHKLYVTLA
                     QMLEKSIHEFEMDRLILMGNDWQIKLFENYLSRPLRQLIVGYATNPANPENPSAKDIL
                     DRISPIMREAEEQEEMQLINEAQGPKGVSGLQNVLEALQMGQLQVLILPWDLNVSVYR
                     CSDGIIFATPEEAKDYDDSFEEIPLKKEIFYLAAKYATRLEFVNNVPKEKLYNELEGI
                     AGILRW"
BASE COUNT         2153 a         1124 c          899 g         2572 t
ORIGIN      
        1 atcgagagaa actttatcga acaaagcagt gtattctttt atagcctgat cgccatcttt
       61 gtgcactttg tttaaaattt tctgaacagt ttcaaacaaa gctcttcgat caattactgg
      121 acgagataat atagatgccc atttttcttg ttcaggatat ttaaatattt tcataatcta
      181 atttaaaata gattacaaat taaatacttt tttgttagaa atgagcaata aatctaccac
      241 tattttatta tcctgatttt cacattcttt tttatcaaag tattacacaa attgtgacga
      301 atagctattt ttaaaaagtt tatgaagaca ggaagtgagt tcatctataa ttcttgatcg
      361 taccatatca tatcttagtc aagttctacc gacattttta caacttataa ttctcagttg
      421 taccatcaaa agatttttgg tcgtggaaga ttcaaatcat cacttataaa aaacaaaaaa
      481 ccttttgtat tttcaaatac ttaaaagtgg atatttttgt aaaatgcccc taattaacat
      541 actatttgtt aaaatcaaca aaactaccga catcatctaa attcattgta atccttttga
      601 gacgaatgaa attaaagttt agctgaaatt tatcattaag gataattaat tcataataaa
      661 gaaattcctt aaaattgact atcctcaccg caagcagcga ggtattacgc caattttatg
      721 gcttcgccac cggaatttct aaaaaaataa aaacaaaagc taccccgacc aagcatcggg
      781 gaattcttac gattaaaaat tcttgattaa gaaaagatgt gaatttacaa atttgatttt
      841 ttacaaaata aaaaagtccg ttgtagttaa aaaacggact tactattaca gaactacttt
      901 tgttcctaat tattacatag gagctacagt agtatcaaca gttgtagtgt caatactttc
      961 aacaatagta tcaactgggg caactacact tgtggtgtcg gtttctgtgc tttcctgagt
     1021 agcttttttc ccacaagcaa caaatgcaaa aactacagca aataacaata ctaatttttt
     1081 catttttttt tatttttaat ttaacatgtt aatgttatat tctaaaacga tgcaaagtta
     1141 catctttttt tttatttaat taaataaatg tcattttttt aattaaactt cgaaaagtta
     1201 attactcggt ataaaattta ttccttaaaa attaaacttt tgaaaataga atcctctata
     1261 tgaatggttt tttattattt atagtctgaa caattattca aatggagttg ccatagtgtc
     1321 aatgacattc gttgtgtcta tttgtgattc tatggtatct acaattacta tagaatcttc
     1381 actttcagct ttttcttcag tagctttttt cccacaagca acaaatgcaa aaactacagc
     1441 aaataacaat accaattttt tcatttttca caatttttta aaagattaat tttttaacca
     1501 ctattcaaaa aacgatacaa atatacaact tttttgtata tgttgttcat aaaacttatt
     1561 ttttagaact tttttgttgt tttgttataa aaaattgatt ttaatcattt tatcttctaa
     1621 ttcaacggat tgacaaacca tttattttat tatttagaac aaaaaataaa aatgaaatga
     1681 aaataaatgt taattaaaaa taaatatgtt ttaagataaa ttagaaaatg cagaaatttg
     1741 tttttgagac cactctttaa aaaactattt ttattttttt taaaaatcaa tattgttatt
     1801 caattttgaa tttttattca aaatttacaa cagtaattcc agagccacct aattcgatat
     1861 gttcatcatg aaaatttttt atttgtggaa cagtagcaag atactgacga atcaactgcc
     1921 gcaatactcc actacctgtt ccatgtaaaa tacgcacaga attggctccc accataatgg
     1981 catcgtcaat aaaataactt actgcttgca cagcttcttc tcctctcatt ccacgaacat
     2041 caatttctgt tttgaaattt agtttcctgt tctttatttc ttcgtttttt aattcaatag
     2101 attgttgaat tttgttgatt tctttttttg tttgtgtgcg attgataaat tcgagtttag
     2161 aaagttctat tgttgatttc atttgaccaa aagctataat ggcttgttcc tcactgattt
     2221 ctataattct tccaacaaga gagtgtcctt ttatttttac ataattacct acttggaaag
     2281 tggattttga taaattttcc gaagcaactt tttttgattt tttccttttt aattgaatgg
     2341 aaggttctat tggtttaggt aaaatttctg gttccagttt ttctttctta aaagtttcca
     2401 attcttttct tatctccttt gttttttctt tttctgcttg agcttcctta atctggcgta
     2461 tagtattttc aatttttgca ttagcttcat tcaataattg gatggcttcg gcttttgtag
     2521 cttgaataat ttctttttgt tttttttcaa tattatttaa tgttgtttcg tattgctcga
     2581 taattttttc gagatatttt tctcgttgac ggattttctt tcgcttattt tcccaataac
     2641 gtttatcccg aataatttca tgcaaatgtt tttcgtaatc aaaatgttct ttcccaacct
     2701 tttgagcagc ttcttcaata atatcgtcag gcaaacctat ttttcgagct atttcaatcg
     2761 caaaagaact tccaggaaaa ccaatttgca gttgaaaaag tggttgcaat cgtccgcgat
     2821 cgaacaacat agctccattt accactcctt cggtgttctc ggcaaaatgt tttaaatttg
     2881 tgtaatgggt ggtaatcaca ccaaaagctt tttgctgatt aattctttcc aaaacagctt
     2941 cagcaatggc tccaccaatt tgaggctcag taccagagcc aaattcgtca atcataacca
     3001 gcgttttttc attgctgtgc tgaataaaaa atttcatgtt cagcaaatgg gaactataag
     3061 tagaaaggtc attttcaata gattgttcat cgccaatatc aataaaaaga ttttcaaaaa
     3121 ttcctatttg gctactctcg tccagtggaa ttaacaaacc acattgaagc atatattgaa
     3181 gtagtcccac agttttcaag caaacagact ttccgccagc atttggtcct gaaataatca
     3241 aaattcgctg attctcattt aaaacgatat ctaatggaac aatatttttc ccttgctttt
     3301 gcaaagaaag gtataaaagc ggatggctgg ctttgttcca ttgaatgata catttatttt
     3361 caacatttgg acgaatagct ttaatatcaa tggcaaaaag agcttttgaa tgtaggaaat
     3421 ctatttcacc taaaaaattt tgaagctcaa gcaaatctgg aataaaaggg cgaatataat
     3481 ttgtaatatc tgttaaaatt ctaataattt cacgccgttc ttctccttcg agttcacgaa
     3541 tgctattgtt caattctatt acttcagcag gttcaatata gactgttttt ccactggctg
     3601 attcatcatg tacaatgcca ttgattttcc gtttaaacgc ttgtgaaaca ggtataacca
     3661 gcctcccttc gcgtaaagta ggcaaagtat ccttttctac atagccattc gaaaaagccc
     3721 gctgtaatat ctcgtttaaa gttcgagaaa tattattctg tgtagttaaa atattttttc
     3781 taattttttc taattgtggt gaagcattgt cttttatatc gccatatttt gtaagaattt
     3841 tatttatctg atcaattagg aaagaaaagg attttacagt tttagacagg cgtgacaatt
     3901 gggggtaaaa agtagaattt aatttagaaa aataggtcgt taaaagttgt gcgttttcaa
     3961 gatttgtacg aagacaatat aattcatttt cagttaaata taaaccttct acatgaattt
     4021 tatgtaatga tggacgaata tcataaatat tgcttaaagg caattcgttg gtttcagtag
     4081 taaggatttg aagcatctca ttggtttgat ccaactgttg aattattttg taaaaatcag
     4141 acgaaaaatg tatgctgtca attttttctt ttcctaaagt gctgatactt ttttctttta
     4201 ataattgccg aatattccca aaatcaattt tttgctcgaa attttgcgga taaaccatct
     4261 tttaaattct catttggatt ctaatttttt tgcttcgttc catatttcgt ccatttcctg
     4321 caaagtcatt tgtttcaaat cttttccttg agaaatagtt ttagattcca gataattaaa
     4381 acggttgata aattttcgat tggtgcgttc caacgcattt tctggattaa tgtcgtaaag
     4441 ccttcccgca tttattaaac taaaaagaag atccccaaat tcttcttcta ttttatcttt
     4501 gtccagtgat ttgatttctt gttgcaattc taaaaattct tcttttactt tttcccatac
     4561 ttgctctttt tcttcccaat caaaacctac atatcttgct ttgtcctgaa ttctatgagc
     4621 tttaatcaaa gctggcaatg aatcaggaac accacctaat actgttttat taccattttt
     4681 ttcttttagt ttgagttttt cccagttttg aactactgtc tcggtagaat cggacttaac
     4741 attagaaaaa acatgaggat gtctgtaaat aagtttttca ttcaatgctt tacaaacttc
     4801 gtaaatatca aaatcattat tttcatttgc catttgagcg taaaaaataa tatgaagcaa
     4861 taaatctcct aattcttttt taatttcttc catatctttt ttcatgatag catctgacaa
     4921 ctcgtaagtt tcttcaatgg tttgcaccct tagactgtcg aaagtttgtt ctttatccca
     4981 gggacatttc tgacgaagtt cattcattat ttccacaagt ctgccaaatt cttctttaat
     5041 ttcatcaagc gtacgttttt tatcttccat aaattatttt aaaatgaaat attaacttaa
     5101 agatgactta tttttattgg cgtgttattt tctcaatatt ttaaaaatat cagccaatgt
     5161 cgataacaag ttatataaga acaaaattat ataaattctg tttacgaaga gaattctttt
     5221 ttaaaggaac aaaaaaggga aaatatttca aaaaataatt tccctcgttt tttaatcttt
     5281 tctgaaaagt taaactatta tttaccagcg taatatacct gcaattcctt ccagttcgtt
     5341 gtataatttt tcttttggga cattatttac gaattccaat cgtgtagcat atttagcagc
     5401 gaggtaaaat atttcttttt ttaaaggtat ttcttcaaat gaatcatcat aatcttttgc
     5461 ttcttctggt gtggcaaaaa ttatcccatc gctgcatcga tatacggaaa catttaagtc
     5521 ccacggcaaa atcaaaacct gaagttgtcc catttgaagg gcttctaata cattttgtaa
     5581 accagaaacg ccttttggac cttgtgcctc attaattaat tgcatttctt cttgttcttc
     5641 agcttctctc attataggag agattcgatc taaaatatct ttagctgaag gattttcagg
     5701 atttgcagga ttagttgcat aacccacaat caactgacga agaggacgac ttaagtaatt
     5761 ctcaaataat tttatttgcc aatcattgcc catgagaatc aatctgtcca tctcaaattc
     5821 atgaattgat ttttcaagca tttgagctaa tgttacatac aatttatgag agaaagtctg
     5881 ttgtttcgct tttattttat ctttaaattt cattttagca ttaaacattt tatcttctaa
     5941 tactcctgat tcgatgattt gagaatactc attgaattct ttccattcat ttggagtaat
     6001 ggctgcaaaa acatcagtta cttccttgac ttcattcata aatacttcat aaaatctcca
     6061 tttgttgcca taaatgtgta acacgcctac ccttctaaac tcgtcaaaag cataaaggat
     6121 aggagttaaa taaggtttac caaaatgagc ttctactcta ccacttcgta aatcaactac
     6181 tggtaaatcc acctgtaatt caaaatgttc tactgctaat tctccttttt cgtttatcca
     6241 cgcaaaaagg accaaagtac gagcttctgt tctaatttga tctaaaaaaa ttattaattc
     6301 ttcatagaaa ctttttcctt ttttaagctt aatattaagc tcattcattg attttaaagt
     6361 attttttaat cgatcatacc atgctcttcc cacattttca ggaatagcag gattaacatt
     6421 gcaatatact gaaagcacag gttcatttat gctgctcact aatgttttaa atcgttgaat
     6481 gtcatttttg cttatcatat atctacattt tttgattgaa ttttgttatt attttatata
     6541 aattttcatg ataatttaca atcggtttac agaaaaaaat aaagtaccac aacccttgca
     6601 atttagcgag cttactttaa aaattaaaag acatgacata gcactttttt agtatttgca
     6661 aaattacatg aaaatcgagg tatttccaaa cgtatgaaaa taaaaatcaa ggtcacaaga
     6721 ctttaaaggt ttaccgtacc gtgcttct
//
LOCUS       DAOTKS010000029         6642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_10926_length_6642_cov_3.965690, whole genome shotgun sequence.
ACCESSION   DAOTKS010000029 DAOTKS010000000
VERSION     DAOTKS010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6642
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10926_length_6642_cov_3.965690"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            384..1229
                     /gene="panC"
                     /locus_tag="P5236_01130"
     CDS             384..1229
                     /gene="panC"
                     /locus_tag="P5236_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRU62998.1"
                     /translation="MKVVLTKNELQSEIEKIKGKKKSIGLVPTMGALHKGHLSLIEHC
                     INENDISVVSIFVNPTQFNNKKDLEKYPRNVAKDIQLLDDLGCTLVFTPSVEEMYEDK
                     ELNQPFEFDFGGLDTMMEGKFRPGHFNGVVQIVSKLFQLVQPDRAYFGEKDFQQLAII
                     RYMVKKMKFDIDIVPCPIVREANGLAMSSRNELLTPEQRKIAPEISKILFASRELVPE
                     LSPSAIENWVTEKLNNIPAFKVDYFEIVNAETLLPVQSWKESAIGCVAVYCGEVRLID
                     NVRYN"
     gene            1275..1625
                     /locus_tag="P5236_01135"
     CDS             1275..1625
                     /locus_tag="P5236_01135"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HRU62999.1"
                     /translation="MLVSILKSKIHRVTVTEANLNYKGSITLDEALMEAANLLPNEKV
                     IVVNNDNGERIETYVIPGKRGSGIVCLNGAAARKALPGDIVIIMSFALMEIEEAKLFK
                     PTIIYPDTATNQLI"
     gene            1634..2221
                     /gene="hisH"
                     /locus_tag="P5236_01140"
     CDS             1634..2221
                     /gene="hisH"
                     /locus_tag="P5236_01140"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRU63000.1"
                     /translation="MDVAVIKYNAGNIRSVLFALERLQVNTIVTDEEDKIRSADKVIF
                     PGVGEASSAMAYLKDKKLDKVILSLIQPVLGICLGMQLMCSFSEENNTPCLGIFKETV
                     KHFPQNSYKVPQIGWNNIYDLSNTLFSGIAENSYVYFIHGFYVEVSKYTIAKANYMLE
                     YSAALHKNNFYAVQFHPEKSGEIGQKILENFLFQV"
     gene            2252..3382
                     /gene="tgt"
                     /locus_tag="P5236_01145"
     CDS             2252..3382
                     /gene="tgt"
                     /locus_tag="P5236_01145"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRU63001.1"
                     /translation="MFFEVLHNDVDSNARIGKITTAHGVIETPIFMPVGTAGSVRAVH
                     FRELKEDIKAQIFLGNTYHLYLRPGINILEEAGGLHKFTGWDLPILTDSGGYQVFSLS
                     EIRKLHEEGVMFRSHIDGSKHLFTPENTVDIQRSIGADIIMALDECTPGTADYQYAKK
                     SMELTHQWLKRGIKRFDETQPKYNYFQAFFPIVQGCIYPDLRKESAEVIAAENRDGNA
                     IGGLSVGEPTEKMYEMIEIVNEILPKNKPRYLMGVGTPQNILESIQRGIDMFDCVMPT
                     RNGRNGMLFTLQGIINIKNAKWKNDFSPLDEFGTSYVDKVYSKAYLHHLFINKEILAL
                     QIASIHNLAFYLWLMAEARRHIQNNDFTQWKKSIIQQLGERI"
     gene            3431..4531
                     /locus_tag="P5236_01150"
     CDS             3431..4531
                     /locus_tag="P5236_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRU63002.1"
                     /translation="MKVDYQKIGLKRIDTYIINKFLGTFFFCMVLILSISVIFDLTEK
                     LDNFFESNAPLKAIVFDYYLNFIPFYLNMFTPLFTFIAVIFFTSKMAGDNEIIAILSS
                     GVSFNRLMLPYFLSATFIALFSFGLGGYVIPHSTKKMLDFENQYVKKFKPENARNVQM
                     EIEKGVILYIERFEITTNRGYHFSLEKFDGKKLVSRLTAESIKWDSAYHWTITNYLQR
                     DFVGMHEKIKKGDVLDTVINVQPKEFFITSKESAQLNNAELRNYLHRQRERGVGNIQA
                     FENEYQKRYSMPFAAFVMTLIGVSLSSRKVRGGMGLHLGIGIALSALYILFSTISTTF
                     SVSGVMSPFLAVWLPNFVFILIGFYLYSTAPK"
     gene            complement(4604..5470)
                     /gene="speE"
                     /locus_tag="P5236_01155"
     CDS             complement(4604..5470)
                     /gene="speE"
                     /locus_tag="P5236_01155"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002010.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HRU63003.1"
                     /translation="MLEKNYPTVTFNEYDEINEYGVTIGINKIIYSEQTKYQKIDIFE
                     TKNFGKIFTLDGIVMTRDSDEFIYHEMISHVPLFLHDNPKNVLVIGGGDGGTVREVLK
                     HSSVEKVTMCELDEKVVEASKKYLPNLSYELTNPKVKLIFDDGSNFIKKFKNKFDVII
                     IDSTDPTEEGGGILYTEEFYRNCSEALTENGVFSAQTEEPFLEKVWMNKAYKNISSVF
                     NIARLYMGFVPAYFPGTWTWTFASKNIDPIKDFKAEKVTNFKEKLKYYNEEIHIASFA
                     LPNFVKEIINVI"
     gene            complement(5478..5876)
                     /gene="speD"
                     /locus_tag="P5236_01160"
     CDS             complement(5478..5876)
                     /gene="speD"
                     /locus_tag="P5236_01160"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HRU63004.1"
                     /translation="MTKSLGKHLIAELYDCDEKILSNVSEIENQMSKAAIECGATIVA
                     STFHHFSPHGVSGMIIISESHLAIHTWPEYKYVSLDIYTCGNSVDPWIAFHNLKDAFK
                     SKRQEAVEYKRGIFKTLGIPEDSSHKVELK"
BASE COUNT         2227 a          975 c         1087 g         2353 t
ORIGIN      
        1 aaatgtacaa tacttttgat acttgattca ttattcccaa ctaaaataat tcatttatta
       61 tgcaaagata caaaaaaaat cagacatttg cacttgaagc gtcttaaaat aaataactaa
      121 tgagtgttaa atagtagaat aaaacttcaa tttttgactc tgaaaaattt tttatgtaaa
      181 ataagcgact ggattttttt aaaattatca tcaaataatt ttgttccaat ttattgtaaa
      241 acaggaactt cggagaggaa gaaattcgat ttgtttacaa aaaagaaaat aagcaaagaa
      301 attttagtaa ttttgcattg tttttcaaaa caggaaaatt tgactgaaca aaaactgtct
      361 aatgaataca ttttgattca ataatgaaag tagttttaac taaaaacgaa cttcagtcgg
      421 agattgaaaa aataaaagga aaaaagaaaa gtataggttt ggttccaacg atgggagctt
      481 tacataaagg acatttatcg ttgattgaac attgtatcaa tgaaaatgat atttctgtgg
      541 tgagcatttt tgtcaatccc actcagttta acaataaaaa agatctggaa aaatatccac
      601 gtaatgtcgc aaaagatatt caattacttg atgatttagg ttgcactctg gtttttacgc
      661 cttctgttga agaaatgtat gaggataaag aactgaatca gcctttcgaa tttgattttg
      721 gtggacttga tacaatgatg gaaggtaaat ttagaccagg acatttcaat ggcgtagtac
      781 aaatagtgag caaattattt cagttagtgc aacctgacag agcttatttt ggagaaaaag
      841 actttcagca attagctatt attcgttata tggtaaaaaa aatgaaattc gatattgaca
      901 ttgttccttg cccgattgtt agagaagcaa atggtttagc catgagcagt cggaatgaac
      961 ttttaactcc agaacaacgt aaaattgctc ctgaaatttc caaaatatta tttgcaagcc
     1021 gtgaacttgt ccctgaactt tcaccttcag ctattgaaaa ttgggtaacc gagaaattaa
     1081 ataacattcc agcttttaag gttgattatt tcgaaattgt caatgctgaa accttgcttc
     1141 ctgttcaatc atggaaagaa tcggctatag gatgtgttgc agtatattgt ggcgaagtaa
     1201 ggttaattga taatgttaga tataattgat tcctttcttt tgaaaaaatt aatccaaaaa
     1261 attaatccaa aattatgtta gtgagtattt taaaatctaa aattcataga gtcacagtaa
     1321 cagaagctaa tttgaattat aaaggcagta ttactcttga tgaagctctg atggaagcag
     1381 ccaatctact tcctaacgaa aaagtcatag tagtcaataa tgataatgga gaaagaattg
     1441 aaacgtatgt tatacctggc aaacgaggtt cgggtattgt ttgcttgaac ggagctgcag
     1501 ctcgtaaggc tttaccagga gacatagtta tcattatgtc ttttgctctt atggaaattg
     1561 aagaagctaa actgttcaaa cctactatta tatatcctga cacggctact aaccagctca
     1621 tttaactttt tatatggatg ttgccgtaat aaagtataat gcaggaaata ttcgttcagt
     1681 actttttgct ttggaacggc tacaggtgaa tacaattgtt actgatgaag aagataaaat
     1741 ccgcagtgct gataaagtga tttttccagg cgtgggagag gcttcttcgg caatggcata
     1801 tcttaaagac aaaaaactgg ataaagtgat tctttcactg attcaacctg ttttaggcat
     1861 ttgtttggga atgcaattaa tgtgctcttt ttcagaagaa aataatacac cttgcttggg
     1921 tatttttaaa gaaacagtaa aacattttcc tcaaaatagt tataaagttc cacagattgg
     1981 ctggaataat atttacgatt tgtcgaacac gcttttttca ggaattgcag aaaattccta
     2041 tgtttatttt atacatggtt tttatgtaga ggtatcaaaa tataccattg ccaaagcgaa
     2101 ttatatgttg gaatatagtg ctgctttaca taaaaataat ttttatgcag tacaatttca
     2161 tccggaaaaa tctggtgaaa ttggtcaaaa aattttggag aattttttat ttcaagttta
     2221 aaaattatgt gtttggttaa cttgtgaaca aatgtttttt gaagttttac ataacgatgt
     2281 tgacagtaat gcccgtattg gaaaaattac cactgcacat ggcgtcatcg agacgccaat
     2341 ttttatgcca gtagggacag caggttctgt tagagctgta catttcagag aattaaaaga
     2401 agatattaag gcacaaattt tcttaggaaa tacttatcac ctgtatttgc gtccgggaat
     2461 aaatatttta gaagaagctg gtggattaca taaatttacg ggatgggatt tgcccatatt
     2521 aaccgatagc ggaggttatc aagttttttc gctttccgaa attcgtaagt tgcatgaaga
     2581 gggtgtgatg tttcgttctc atattgatgg cagcaaacat cttttcacac ctgaaaacac
     2641 tgttgatatt caacgttcta ttggagcaga tattataatg gcacttgatg aatgtacccc
     2701 tggcactgct gattatcaat atgcaaaaaa gtcaatggaa ttaactcatc agtggctaaa
     2761 acggggaata aaacgttttg atgaaactca acccaaatat aattattttc aagctttttt
     2821 ccccattgtt caaggatgta tctatcctga cttacgaaaa gaatcggctg aggttattgc
     2881 tgctgaaaat agagatggga atgctattgg aggattatca gtgggtgaac caacagaaaa
     2941 aatgtatgaa atgattgaaa ttgtaaatga gattcttcca aaaaataaac ctcgttactt
     3001 aatgggagtt ggaacgcctc aaaatattct ggaaagtatt cagcgaggca ttgatatgtt
     3061 cgactgtgtg atgcccacac gaaatggtcg aaatggaatg ctttttacct tacaaggaat
     3121 tataaatatt aaaaatgcta aatggaaaaa tgatttttct cctctcgatg aatttggaac
     3181 atcttatgta gataaggttt actcaaaagc ttatttacat cacttattta tcaataaaga
     3241 aatattagct ttgcagattg cttcaattca caacttggct ttttatttat ggctgatggc
     3301 tgaagctcgt cgtcatattc agaataatga ttttacacaa tggaaaaaaa gcatcattca
     3361 acaattgggt gaaagaatat aaaaatattt tggagaaaca tccactaaaa gaaaattttc
     3421 aataaagcga atgaaagttg attatcagaa aattgggctt aaacgaattg acacctatat
     3481 tattaataag tttttaggta cttttttctt ctgtatggtt ttgatattaa gcatttctgt
     3541 tatttttgat ttaacagaaa aactggataa tttttttgaa agcaatgctc cattaaaagc
     3601 aattgttttt gattattact tgaattttat tcctttctat ctgaatatgt ttactcctct
     3661 gtttactttt attgccgtca tttttttcac ttcaaaaatg gctggcgaca atgaaattat
     3721 tgctattctt tccagtggag taagttttaa tagattgatg ctaccctatt ttctttcggc
     3781 tacttttatt gctctttttt cttttggatt aggaggttat gttatccctc attccaccaa
     3841 aaaaatgctt gactttgaaa atcaatatgt taaaaaattc aagcctgaaa atgctcgaaa
     3901 tgttcagatg gaaattgaaa aaggcgtgat tttatatatt gaaagatttg aaattactac
     3961 caatagaggt tatcattttt cgctggaaaa gtttgatggg aaaaaactgg tttccagact
     4021 tactgctgaa tcgatcaagt gggactcagc ttaccattgg acaattacca attacttaca
     4081 aagagatttt gtcggaatgc atgaaaagat taaaaaagga gatgttctgg atacagtaat
     4141 aaatgtccaa cctaaggaat tttttattac ttccaaagaa agtgcacaac tgaataatgc
     4201 agaacttcgg aattatttgc accgacagcg tgaacggggt gtgggtaata ttcaagcatt
     4261 tgaaaatgag tatcaaaaac gttactctat gccatttgct gcatttgtaa tgactttaat
     4321 cggtgtttcc ttatcttcaa gaaaagtacg aggtggaatg gggcttcatc ttggcatcgg
     4381 aatagcactg agtgctttat atattttatt ttcaaccatt tcaactactt tttcagtaag
     4441 tggagttatg tcaccatttt tagcagtgtg gctaccaaat tttgttttta ttttaattgg
     4501 tttttatttg tattctacag caccgaagta attttataag aaagaaataa taccaatttg
     4561 ctttcaaaaa tagaaaaata tttttttgat ttctgaccag aactcaaata acatttatta
     4621 tttctttaac gaaatttggt aaagcaaatg atgctatgtg aatttcttca ttatagtatt
     4681 ttaatttttc cttgaaattt gtaacttttt cagctttaaa atcttttatg ggatctatat
     4741 tttttgaagc aaatgtccaa gtccatgttc caggaaagta tgctggcaca aatcccatgt
     4801 ataatcttgc aatattaaat actgaactga tatttttgta agctttattc atccatactt
     4861 tttctaaaaa tggttcctct gtttgagccg agaaaacacc attttcagta agtgcttcag
     4921 aacaatttct ataaaattct tccgtgtaaa gtattcctcc accttcttct gtcggatccg
     4981 ttgaatcaat aattattaca tcaaatttat ttttgaactt tttgataaag ttacttccat
     5041 catcaaatat caatttaact tttggattgg ttaattcgta cgataaatta ggtaaatatt
     5101 ttttagaggc ttctacaact ttttcatcta actcacacat tgtgactttt tctactgacg
     5161 aatgtttcag tacttctcta acagttccac catctccgcc tccaattaca agtacatttt
     5221 tagggttatc atgaaggaat aaaggaacat gagaaatcat ttcatgataa ataaactcat
     5281 ctgaatctct ggtcataaca attccatcaa gagtaaatat tttcccaaag ttttttgttt
     5341 caaaaatatc tattttttga tatttagttt gttcagaata gatgatttta ttgataccaa
     5401 tggtaacacc atactcatta atttcatcgt attcgttaaa agttacagta ggataatttt
     5461 tttctaacat cattgtttta ttttaattca actttgtgag atgaatcttc tggtattcca
     5521 agagttttaa atattcctct tttgtattca acagcttctt gtcttttgga cttaaaagca
     5581 tcttttagat tatgaaaagc tatccaggga tcaactgaat ttccgcacgt ataaatatct
     5641 aaagaaacat atttgtattc aggccatgta tgaattgcca aatgagattc tgatattatt
     5701 atcataccac ttacaccatg aggtgaaaaa tgatgaaagg tggatgctac tatggtagct
     5761 ccacattcaa tagctgcttt tgacatttga ttctcaattt ctgaaacgtt gctaagtatt
     5821 ttttcgtcac aatcgtataa ttcagctatt aaatgtttgc ctaaagattt tgtcattcct
     5881 tttccctcct ttaaaatttt tataaataaa gatttcactt taaagtataa aaaattataa
     5941 cgtgatttat atcgctaaaa gtttaaaaat taaaaaaata gatttaatca cgttatttta
     6001 taaatgcaaa tataattgaa tttttttaat ttgagaggtg aatctataat atgacatcat
     6061 ttgttactaa tttgtttttc tattaacctt ttccattatt ttttgattta gttttttggg
     6121 ggatatattg catccctaac ttcgcaaaat ttacgtaata ggtagttcca attttttcaa
     6181 aaggctgaca gctttcttgg taatttcagc aatatatcat ttgttgttga ttttccttgt
     6241 tttatttcag ttaaggaata aatcaaaaat ctttaggtga aaattccccg ttcagttcat
     6301 gctttgtcag caacaccata tttgataata ccaagataaa gcattagtta tcgtttattt
     6361 tcccaaatat aaacgaaacg cctttacgtc tttgatattg acctgattta ttctgtgtaa
     6421 taagttgtta ttctactgaa aaaaacaaat attaaattaa taatcgatcc atctcgtttg
     6481 cttttccgat tcaataaatc catccttttc agtaattcta tcaagaagtt ttcctgttac
     6541 ttaagaaaaa tccgttagaa agttctaatc taataacaaa tttgggtgaa aacaattcca
     6601 gattcttatt gataataaaa tatcttgaaa gtttaatact ca
//
LOCUS       DAOTKS010000030         6636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_10938_length_6636_cov_5.003039, whole genome shotgun sequence.
ACCESSION   DAOTKS010000030 DAOTKS010000000
VERSION     DAOTKS010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6636
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10938_length_6636_cov_5.003039"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="P5236_01165"
     CDS             complement(<1..845)
                     /locus_tag="P5236_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005648316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HRU63005.1"
                     /translation="MVKVKPFKGIRPPKDLVEKVASRPYDVLNSEEARVEAEGNPMSL
                     YHIIKPEIDFECGTDEHEEKVYLKAVENFAKFRENGWLQKDKTEHYYVYAQTMNGHTQ
                     YGIVLCAYIDDYLTGKIKKHELTRKDKEEDRMKHIRMTNANMEPVFLAHLFQPELDKI
                     VSEVIKNKPEYDFKAKDGIIHSFWIIDDKDKMKKITEIFDNIPSLYIADGHHRTAAAV
                     AVGNEKKQSNPSHTGNEEYNYFMAVCFPENQLRIMDYNRVVKDLNHLTDEEFLKKISE
                     YFLVE"
     gene            1071..2027
                     /locus_tag="P5236_01170"
     CDS             1071..2027
                     /locus_tag="P5236_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRU63006.1"
                     /translation="MRILVSTKLPLEGFGSLIEKFEVVFPESDIFSKEEVIQIIPSFD
                     AFISTFQFKVDKEVIDAGAAGRLKIISNYGVGYDNVDVEYAAKKGIVVTNTPDPVTEP
                     TAEHTIGLMLAAARRISECDRKLRIPNGLKWGLLENLGQSLYGKTLGIIGMGRIGKAV
                     ARRALAMDMKIIYYQRNRLDPTLEEQFKAEFIEQKTLLSESDVVTLHLPLNENTYHLM
                     NEETLRLMKSTAILINTARGPIVDEIALLKALKNHWIAAAALDVFENEPDITSGLLEL
                     DNVVLSPHNGTATLEARNEMSKFAAQNIINFFEGNGNITKVN"
     gene            2107..2655
                     /locus_tag="P5236_01175"
     CDS             2107..2655
                     /locus_tag="P5236_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRU63007.1"
                     /translation="METGEEELMFQLNQANTKAAAFSMVVRKYQERLYWHIRKMVLSH
                     DDANDILQNTFLKAWEGIDNFRGDSLISTWLYRIATNETLTFLANKRMQNITSMDELE
                     EVLAQNLEADKYFDGDEAQLKLQKAILTLPENQRLVFNMKYFDDLTYEEMEAILDTSV
                     GALKASYHIAVKKIEKYLTEEE"
     gene            2778..3122
                     /locus_tag="P5236_01180"
     CDS             2778..3122
                     /locus_tag="P5236_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63008.1"
                     /translation="MKKQTHISLEEIGKKLPFKVPENYFEEFASQIDEQIAPKKPSWK
                     QISQPWLYLVALFVGILFMVQIFYTIYVKIEKKQKENYELYVLSQIDEPFIMNYYLTN
                     EENNLSGNENEY"
     gene            3106..3582
                     /locus_tag="P5236_01185"
     CDS             3106..3582
                     /locus_tag="P5236_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63009.1"
                     /translation="MKMNIKYVIFFCLLFIGTTLQAEEARKNPSIKDMHEKKWSYLVK
                     KAQLTPQEAAIVKPIFMEYEQSLWQLHSQARDTFKKNYMKGENPQINYEELNELYINV
                     QLKQAELLKKYHHQLRKVLSAKTLFNYYQAEQSFKRQLLRHMPKQKNNKALKQPQE"
     gene            3663..3733
                     /locus_tag="P5236_01190"
     tRNA            3663..3733
                     /locus_tag="P5236_01190"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3695..3697,aa:Cys,seq:gca)
     gene            3884..6562
                     /locus_tag="P5236_01195"
     CDS             3884..6562
                     /locus_tag="P5236_01195"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRU63010.1"
                     /translation="MKIRFTIHYVTQWGQTISVFILSDQNKNPQQFLMTCNKDFEWSI
                     EIEFKTPLKFIQYRYGLLHPDSKIEYEYGEYRYLNMDTSYSNINILDNWRVRFGDSPF
                     ETTAFLDCFFRRENQFTQPLKDKNANLLLQLRCAQIEPDRHFAIVGNQEALGNWDVRK
                     KIRLDDSDFPIWKISLNASKFKFPLEYKYLIVDTETDEVLAWGGGPNRKITTIFNKTL
                     NIVNDEHFIRTIPSWHGTGVSIPVFSLRSEESFGVGEFNDLKKMVDWAKMTGQRIIQI
                     LPVNDTIVYHTNYDSYPYNAVSVYALHPIYLHLENLGMLKDEKQNEYFVQKKKEFNAK
                     TFSDYQNVLNVKWEYFKMIYTQKKSKIFSSEDYQTFFETNKRWLMPYAAFSYLRDVYG
                     TPNFREWKNFSCFDLKEIENFSSPEQPHYDEIAFYYFLQYHLHKQLSDVHQYARANGI
                     AIKGDIPIGVSPYSVDVWTEPELFNTQVQAGAPPDDFSVIGQNWGFPTYNWEEMEKRN
                     YSWWKNRFQKLAEYFDAYRIDHILGFFRIWEIPNDAIWGLAGNFYPSLPFTREELESK
                     GLAWDENRFLKPYLKEHVLYATFGKYTEEILREYFLPDGWQTYKFKPEYDTQKKIEAH
                     FTSLGYNFSNKDVFIRDGLYALHCEVLFLRDLREPNKFHPRIGMHSTASFRDLPEETR
                     RLLDRLYLDYFYHRHTEFWKQQALKKLPELISATKMLACGEDLGMVPDCVPEVMQLLE
                     ILSLEVQRMPKKPNREFGNPAEAPYLSVCTTSTHDMNPLRAWWEEDPVITQRFYNHVL
                     RLPGEAPKTCETWIVERIIEQHLNSNAMWVILPWQDWIALDEKLRYEHPFAERINVPS
                     NPRNFWCYRMHLTLEQLLKEDDFNHYLKELITKSGR"
BASE COUNT         2311 a         1028 c         1104 g         2193 t
ORIGIN      
        1 ttttcaacca gaaaatattc tgaaattttt tttaagaatt cttcatcagt caaatgattc
       61 aaatctttta ccacacgatt ataatccata attcttaatt gattctcagg aaaacaaaca
      121 gccataaagt aattatattc ttcattccct gtatgagaag gattcgattg ttttttttca
      181 tttccaactg caactgctgc agccgtccga tgatgaccgt cggcaatata aagtgaagga
      241 atattatcga aaatttctgt aatttttttc atcttgtctt tatcatcgat tatccaaaag
      301 ctatgaatta ttccatcttt tgccttaaaa tcatattcgg gtttattttt aataacctca
      361 gatacaattt tgtctaactc aggctgaaaa agatgggcaa gaaaaactgg ttccatattg
      421 gcattagtca tgcgaatatg cttcatacgg tcttcttctt tatcttttcg ggtcaattcg
      481 tgttttttga tttttccagt gaggtagtca tcaatgtatg cacacagcac tatcccatat
      541 tgtgtgtgac cattcattgt ttgagcatat acataataat gttctgtttt atctttctgc
      601 aaccaaccat tttctcgaaa tttggcaaaa ttttctactg cctttaaata cactttttct
      661 tcatgttcat cagttccaca ttcaaaatcg atttctggtt taatgatatg atataatgac
      721 ataggattgc cctcagcttc tactctggct tcttctgaat ttaacacatc ataaggtcga
      781 gaagctactt tttctactaa atctttagga ggacgaatgc ctttaaatgg ttttactttt
      841 accataatta atcagttaaa tttgaattcg caaataagtt tacaaagata aataaaaaaa
      901 tataattttt taaaagtaca aaaacttgct tagttgaatt gtttttaatc aaatgtctgc
      961 taaaaactca aaatttgaaa aatataattg tacacttttt gttgtttcat tacttttgtt
     1021 ttttcttatt tattaaattc tatgaggatt aaatttatta tttcagagat atgcgaatac
     1081 tcgtttcaac taaattacct ttagaaggtt ttggttcttt aattgaaaaa ttcgaagtgg
     1141 tttttcctga aagcgatatt tttagtaaag aagaagttat tcaaataatt ccttcttttg
     1201 atgcttttat ttctactttt cagttcaaag ttgataaaga agtgattgat gctggtgctg
     1261 caggacggct aaaaataata tccaattatg gagtaggata tgacaatgtt gatgtcgaat
     1321 atgctgctaa aaagggaatt gtagtaacta atacacccga tccagtcaca gaacctacag
     1381 cagaacatac cataggttta atgttagctg ctgcacgaag aattagtgaa tgcgacagaa
     1441 aattaagaat ccctaatgga ttaaaatggg gattactgga aaatcttgga caatcattgt
     1501 atggaaaaac gttagggatc attggaatgg gacgtattgg caaagcagtt gctcgacgtg
     1561 ctcttgcaat ggatatgaaa attatttatt accagagaaa tcgcttagat ccaacattgg
     1621 aagagcaatt taaggcagaa tttattgaac agaaaacttt gttaagcgaa agtgatgtag
     1681 taacattaca tcttccttta aatgaaaaca cttatcatct tatgaatgaa gaaacacttc
     1741 gattgatgaa atcgactgct attttaatca atacagcaag aggaccgatt gttgatgaaa
     1801 tagctcttct taaagcactg aaaaatcatt ggattgcagc ggccgcgctg gatgtatttg
     1861 aaaatgagcc agatattact tctggattgc tggaattaga caacgtcgtt ttatcacctc
     1921 ataatggcac tgctacttta gaagctcgaa atgaaatgag taaatttgca gctcaaaata
     1981 ttatcaactt tttcgaaggg aatggcaata taaccaaagt aaattaaact aattgatttc
     2041 tgagcagtca aaatgaataa aaataagaat aaagacaaca acggaagcca ttcaacaaaa
     2101 cttaacatgg aaacaggcga agaagagttg atgtttcaat tgaatcaggc taatacaaag
     2161 gctgctgcat tttctatggt tgtcagaaaa taccaagaac gtttatattg gcatatccga
     2221 aaaatggttt tatctcatga cgatgccaat gatattttac aaaatacttt tttgaaggca
     2281 tgggaaggaa tagataattt taggggcgat tcgttgattt ctacatggct ttatagaatt
     2341 gcaaccaatg aaacattaac ttttttagca aataaaagaa tgcaaaatat cacttcaatg
     2401 gacgaattag aagaagttct ggcacaaaat ctggaagcag ataaatattt tgatggcgat
     2461 gaagcacagc taaaacttca gaaagccatt ttgacattgc cagaaaatca acgactggtt
     2521 tttaacatga aatattttga tgacctcacc tatgaagaaa tggaagctat tttggatact
     2581 tcagtaggag ctttaaaagc ttcttatcac attgcggtaa aaaaaataga aaaatatctt
     2641 actgaagaag aataataata attgtttcga ctatacactt tataaatcaa acaaatctca
     2701 gctaaatgtg ttaaaaaaca atcgatatgc atataaaatt aaactaactc catttttttt
     2761 agtcaaaaaa taaaaaaatg aaaaaacaaa cacatatatc ccttgaagaa ataggcaaaa
     2821 aattaccatt taaagttcca gaaaattatt ttgaagaatt tgcttcacaa atagatgaac
     2881 aaattgcacc taaaaaacct tcctggaaac aaatttcgca accatggctt tatctggtag
     2941 ctttgtttgt agggatatta tttatggtac aaatttttta taccatttac gttaaaattg
     3001 aaaaaaaaca aaaggaaaat tacgaattat acgtactttc tcaaatagat gaacctttta
     3061 taatgaatta ttatttaacc aatgaagaaa ataacttatc agggaatgaa aatgaatatt
     3121 aaatatgtaa tttttttctg tttattgttt attgggacaa cgcttcaagc agaagaggct
     3181 cgtaaaaatc ccagtattaa agacatgcat gaaaaaaaat ggagctattt agtaaaaaaa
     3241 gctcaattaa ctcctcaaga agcggctatt gtgaaaccaa tttttatgga atatgaacag
     3301 tcgttgtggc aactccatag tcaggcacgg gatactttca agaaaaatta tatgaaaggc
     3361 gaaaacccac aaatcaacta tgaagaatta aatgaacttt acattaatgt acagttaaaa
     3421 caagctgaat tattaaaaaa ataccatcat caattacgaa aagtactctc tgctaaaact
     3481 ctttttaatt attaccaagc agaacagagt ttcaaacgtc agttattacg acatatgcca
     3541 aagcaaaaaa ataataaagc attaaaacaa ccccaagagt aagcttttgc ctcgttttca
     3601 ttcccccatt tcaaaacttt gctgaaaaag aaaaaaatat tatctttgca atcccagcat
     3661 aaggtacttt ggccgagggg ttaggcaccg gtctgcaaaa ccgtaaacgg cggttcgaat
     3721 ccgccaagta cctcgcacac cttctttaaa ttgtttgcaa ttcgagaagg tttttttatt
     3781 tttaaaaaat tcccctcttg ttttatcaac tcaacttagg aattttatat atttgttttt
     3841 tcaaaaaaat attactctta aaattatcat ttttaaattt ataatgaaaa tacgttttac
     3901 aattcattac gttactcagt ggggacaaac catctctgta tttattcttt cagatcaaaa
     3961 taaaaatccg caacaatttt taatgacttg caataaggat tttgaatgga gcattgaaat
     4021 agaatttaaa actcctttaa aatttattca gtatcgctac ggtttattgc atccagattc
     4081 aaaaattgaa tatgaatatg gagaatacag atatttgaat atggatactt cttactcaaa
     4141 tatcaatatt ttagacaact ggcgtgttcg ttttggagat agtccttttg aaaccactgc
     4201 atttttggac tgttttttta gaagagaaaa tcaatttact cagccattaa aagataaaaa
     4261 tgcaaatcta ttattacaat tgcgttgtgc tcaaattgaa cctgatcgac attttgctat
     4321 tgttggtaat caggaagcgt tagggaattg ggatgtaaga aaaaaaatcc gtttggatga
     4381 ttctgatttt cctatttgga aaatttcgct caatgcttct aaattcaaat ttccactgga
     4441 gtataaatat ttaattgttg atactgaaac agatgaagtt cttgcatggg gaggcggacc
     4501 aaacagaaaa ataacaacta ttttcaacaa aactttgaat attgttaatg atgaacattt
     4561 tattcgaaca attccttcgt ggcatggtac gggagtgtct attcctgtat tttcacttcg
     4621 tagtgaggaa agttttggag taggggagtt taacgattta aagaaaatgg ttgattgggc
     4681 taaaatgaca ggtcaacgta ttattcaaat tttaccagtt aatgatacta ttgtgtatca
     4741 tacaaattat gattcttatc cttataatgc tgtttcggtt tatgctttac atcctattta
     4801 tttacatctc gaaaacttag gtatgctcaa agatgaaaag caaaatgaat attttgttca
     4861 gaaaaagaag gagttcaatg caaaaacttt ttctgattat caaaatgtgt tgaatgtaaa
     4921 atgggaatat tttaaaatga tttatactca aaaaaagtca aaaattttct cttcagagga
     4981 ttatcagact ttttttgaaa caaacaaaag atggctcatg ccttatgcag cattttctta
     5041 tttaagagat gtttatggca caccaaattt tagagaatgg aaaaattttt cttgtttcga
     5101 cttgaaagaa atagaaaatt tttcatcacc tgaacaacct cattatgatg agattgcctt
     5161 ttattatttt ctgcaatatc atttacacaa gcaactctct gatgttcatc aatatgctcg
     5221 tgctaacgga atagctatta aaggtgatat tcctattggg gtaagtcctt atagtgtgga
     5281 tgtatggact gaacctgaat tgttcaacac acaagtacaa gctggtgctc cgcctgatga
     5341 tttttctgtg ataggtcaaa attggggatt tcctacctat aattgggaag aaatggaaaa
     5401 gaggaattat tcatggtgga aaaatcgttt tcaaaaactt gcagaatatt ttgatgctta
     5461 ccgtatagat catattttag gcttttttcg tatttgggaa attccaaatg atgctatatg
     5521 gggacttgca ggcaattttt atccatcttt accttttaca cgtgaagaat tagaatcaaa
     5581 aggactggcc tgggatgaaa atcgttttct aaaaccttat ttaaaagagc atgttttgta
     5641 tgcaactttt ggaaaatata ccgaagaaat tctccgggaa tattttttac ctgatggttg
     5701 gcaaacctat aaatttaaac cagaatacga tacgcagaaa aaaattgaag ctcatttcac
     5761 ttccttaggt tataatttta gcaataaaga tgtatttatt cgagacggtt tgtacgcatt
     5821 gcattgcgaa gttctttttc tccgtgactt acgtgaacca aataaatttc atccacgtat
     5881 tggaatgcat agcactgctt cttttcgtga tttacctgaa gaaactcgac gtttgcttga
     5941 tcgattatat ttagattatt tttatcatcg acatacagaa ttttggaagc aacaagcttt
     6001 gaaaaaattg cccgaattaa tttcagcaac taaaatgtta gcttgtggcg aagatttagg
     6061 aatggtgcct gattgtgtgc ccgaagtgat gcaactttta gaaattctga gtttggaagt
     6121 tcaacgaatg cccaaaaaac cgaatagaga atttggaaat ccagcagaag ctccttattt
     6181 atcagtttgt actacttcca ctcacgatat gaatccatta agggcatggt gggaagaaga
     6241 tccagtaata acacaacgtt tctataatca tgtgctcaga ttacctggcg aagcaccaaa
     6301 aacttgcgag acgtggattg ttgaaagaat tattgaacaa catctcaact ctaatgcgat
     6361 gtgggtaatt cttccgtggc aagattggat agcgttagat gaaaaacttc gatacgaaca
     6421 tccttttgcc gaacgaatca atgtacctag taatccgcgt aatttctggt gttatcgaat
     6481 gcatttgaca ttggaacaat tattaaaaga agacgacttt aatcattatc ttaaagaatt
     6541 aattacaaaa tcaggaaggt aacaacactt ttaatcataa aaattccccg atgcttgcgt
     6601 cggggtagct tttgttttta tttttttaga aattcc
//
LOCUS       DAOTKS010000031         6601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_10994_length_6601_cov_4.060037, whole genome shotgun sequence.
ACCESSION   DAOTKS010000031 DAOTKS010000000
VERSION     DAOTKS010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10994_length_6601_cov_4.060037"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1466)
                     /locus_tag="P5236_01200"
     CDS             complement(<1..1466)
                     /locus_tag="P5236_01200"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HRU63011.1"
                     /translation="MECKKITTSISQHWSSFLAMMKKVIKYILRILGWIIASIIILLA
                     IAGLLIQTPYVKSKVIDFAEEQVNKSLSARLEVRTLKGNFFTHLQLKDIFLISKNDTL
                     LSVSSLNVKYQLLPLLHGKIVVNELSLEQPHISLLQFADSSWNFQKIIKPQPPKKEKG
                     KPFTMLIDIHHFLLDEALINIHSLNKQLPQQIAHFNIELTGSYQQNHLAVDLKKLNLK
                     TLKPDLQIKNLSFQFAMANKIYKLTDFALQTAKNNVYADGEINLDFSKNSFFHLKTTP
                     LNLNEFETFLPKDFQLKVKPNLQLSTQLTQKELQLLLSVKEQQQKIDLQVVSPSFASV
                     LRSSKKMEPAYLIHLNFQQIDLRQWLTNSSLNIKLNGKIQAQGRGLNPHSMQTNLKGE
                     LTQSVINNNSRIENLNFNFNLQKGNLKGTLTGNSNFGMLSLTTSVDSVLGEYPQYQLD
                     LLTQHLNLAMLTGQNNLNSDLSLQLLLNGAGLKPEQINA"
     gene            complement(1412..3271)
                     /locus_tag="P5236_01205"
     CDS             complement(1412..3271)
                     /locus_tag="P5236_01205"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRU63012.1"
                     /translation="MSDFFKKNIFTRYNCSSGLVFFLFFVYCVEVSAQNGYEIGKIKF
                     NGNKSFSKSELLKQTTLHETKWLDKVLSKKKPVIYNENFIQSDIDRLVRFYQCEGFLN
                     ASVELDSVAQNDKKEKVDLYFKIVENKPVLVGEVKIKIEDSLKVKKDSFFLPFRKRML
                     TVSGKRFSDQKIYNDINLIKDFFQNKGFAYAECDQQIHLQPDLSKADVIFSVKLNEKC
                     FFGNTTIEGNKYVKDQLIYRQCTFDSGQVFNKKKIDKTRKHLFDLQLFRIISLQPLLD
                     NTTKRNPIPVKINVEETSRLSTEFGIGYGTEDEFRAFVDVSYRSLFGDASRLHLYLKH
                     SSLEPYHASLSWIQPQFFSKNFSFSVYPFLLRQKEPGYNIQSYGVNLPLTYSISEKMN
                     TSFGYYMENVKQYEGTTNGDYINPESKDYLYDKSGLMFSYNFNNSYPLTSPVRGNVFN
                     ASVKMNGYIFHSDFNYIRCSFDARRYDQIANFSLAGRILIGGIFSSDSSKFVPVEDRF
                     YSGGSNSIRGWGRSRLGPLRDDGSPMGGKSVLEMSVEVRHRLFWLLEGAMFLDMGNVW
                     NSSFHYPFNQLAYTAGCGLRINSPIGVVRLDVGFPLWNAKKSPQVFLNIGQAF"
     gene            complement(3765..5105)
                     /gene="sufD"
                     /locus_tag="P5236_01210"
     CDS             complement(3765..5105)
                     /gene="sufD"
                     /locus_tag="P5236_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HRU63013.1"
                     /translation="MLMAQQYIDFYKSYQQDIKSHSAILLNAFRDEAFAKFEKLGFPT
                     AKLEDYKYTDMKEPLTVDYGLNINHLPIPVNPYKVFKCDVPGIRSYLYFVVNEVFYPV
                     TSTSNQQFPEGVIIDSLMGASEKYPELVEKYIGKLSREKNDGWIAFNETFAQDGFFMY
                     IPKNVKLKKPVQLVNILHSNVDLMANTRNLIIIEEGAEAQLLVCDHTMDNVKFFSNRL
                     TEIFVDKNAHYEHYHLENVHQQTTNVSTLLVNQQTSSKVLANIITLHNGITRNSTEVN
                     IDGEFCETLLTGMVIGDKTQTVDNFTSIVHKKPNSQSNELFKYILDENSKGAFSGKIY
                     VAPHAQKTFAYQNNRNILLSKNARMRSKPQLEIYADDVKCSHGATIGQLDELAKFYMQ
                     SRGISEAEARFLLMFAFTNDVIENIKIEALKGRMRLLIEKRLRGKLAYCEGCMI"
     gene            complement(5162..5929)
                     /gene="sufC"
                     /locus_tag="P5236_01215"
     CDS             complement(5162..5929)
                     /gene="sufC"
                     /locus_tag="P5236_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HRU63014.1"
                     /translation="MLEIRNLYANINGKEILKGVNLTVKAGEVHAIMGPNGGGKSTLA
                     LAIVGYPGVEVTKGEIIFDGKNLLEISPEDRSHEGIFLSFQNPVEIPGVSMVNFLRTA
                     LNEHRKYKNLEPLSAADFLRLMREKKELVEMDNKLTNRSVNEGFSGGEKKRNEIFQMA
                     MLEPRLAILDETDSGLDIDALRIVANGVNKLKRPDNSIIVITHYQRLLDYIVPDYVHI
                     FYDGRIVKTGDKTLAAELEEKGYDWIKKELEEKELQY"
     gene            complement(6015..>6601)
                     /locus_tag="P5236_01220"
     CDS             complement(6015..>6601)
                     /locus_tag="P5236_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HRU63015.1"
                     /translation="TKRGICKEENAKLSWAQVETGSAITWKYPSCILQGDSSSAEFYS
                     VAVTNHYQQADTGTKMIHIGKNTHSHILSKGISAGYGQNSYRGLVQMSKNADNARNYS
                     QCDSLLLGDKCGAHTFPYMDVSNESAIVEHEATTSKISEDQIFYCNQRGISTENAIGL
                     IVNGYAKEVINKLPMEFAVEAQKLLQITLEGSIG"
BASE COUNT         2174 a         1150 c         1019 g         2258 t
ORIGIN      
        1 gcattgattt gttctggctt taagcccgct ccattcagca ataactgtaa agataaatcg
       61 ctgtttaagt tattctgacc tgttaacatt gccagattca aatgttgtgt cagtaaatca
      121 agttggtact gaggatattc acccaaaaca gaatcaacag aagtcgttaa ggaaagcatg
      181 ccaaaattac tatttcctgt gagcgtacct ttcagatttc ctttttgcaa attaaaattg
      241 aaattcaaat tttcaattct ggaattattg tttatgacag attgtgtcaa ttcgccttta
      301 agattggttt gcatagaatg tgggttcaaa ccgcgacctt gtgcttgtat ttttccattt
      361 agctttatgt taagagaaga atttgtcaac cattgacgaa gatctatttg ctgaaaattc
      421 agatgaatta aataagctgg ttccattttt tttgatgagc gaagcacact ggcaaaagaa
      481 ggagaaacca cttgaagatc aattttttgt tgttgctctt ttactgataa aagcaactgc
      541 aattcttttt gagtcaattg ggtggacagt tgtagattag gcttaacttt tagttgaaaa
      601 tctttcggta aaaaggtttc aaactcattg agattcaggg gtgtagtttt taaatgaaaa
      661 aatgagtttt tagaaaagtc aagatttatt tctccatcag cataaacatt gtttttagca
      721 gtctgtaatg caaaatctgt gagtttataa attttgtttg ccattgcaaa ttgaaaactt
      781 aaatttttta tttgcaaatc tggttttaaa gttttcaaat tcaatttttt caaatctaca
      841 gctaaatggt tttgctgata gctacctgtc aattcgatat tgaaatgagc aatttgttga
      901 ggcaattgtt tattaagcga gtgaatatta atcagagctt catcaagcaa aaaatgatga
      961 atatcaatta acatggtaaa aggtttgcct ttttcttttt tgggtggttg aggctttatt
     1021 atcttttgaa aattccagct tgaatccgca aactgaagaa gagaaatatg aggttgttcc
     1081 aaactgagtt cattgacaac aatttttccg tgcagcaaag gcaataattg atattttaca
     1141 ttcaatgatg aaactgaaag aagagtgtca ttttttgaaa taagaaaaat atctttcaat
     1201 tgtaaatgtg tgaaaaaatt tcctttcaaa gttcgtactt ccaatcttgc agaaagagat
     1261 ttatttactt gttcttcagc aaaatcaatc acctttgatt ttacataagg tgtttgtatc
     1321 aataatccag caatcgccaa taaaataatg atacttgcta ttatccagcc taaaattcgc
     1381 aaaatatatt ttattacctt tttcatcatc gctaaaaagc ttgaccaatg ttgagaaata
     1441 cttgtggtga tttttttgca ttccataatg gaaatcctac gtccagtctt actacaccaa
     1501 ttggcgaatt aatccgtaaa ccgcagccag ccgtataagc caattgatta aatggatagt
     1561 gaaaagaaga attccacaca ttccccatat caagaaacat ggcaccttcc agcaaccaga
     1621 ataatcgatg acgaacctct acactcattt ccaaaacact ttttccaccc attggagaac
     1681 catcatcacg caaaggacct aaacgagacc ttccccaacc tcgaatagaa ttactgccac
     1741 cagaatagaa acgatcttct actggaacaa acttactgct atcagaagaa aaaattcccc
     1801 caatcaaaat acgtccagcc agtgaaaaat tagcaatctg gtcatatctt cttgcatcaa
     1861 aactgcaacg tatataatta aaatcactat gaaaaatgta gccattcatt tttacgctgg
     1921 cattaaatac atttccacgg acaggagaag tcagtggata agaattatta aaattgtaag
     1981 aaaacatcaa gccggattta tcatacaaat aatctttgct ttcaggatta atataatcac
     2041 catttgtagt gccttcatat tgtttcacat tttccatata ataaccaaaa gaagtattca
     2101 ttttttcgga aatagagtag gtgagaggca aatttacacc ataactttga atgttatagc
     2161 ctggttcttt ctggcgaagc aaaaaaggat aaactgaaaa tgaaaaattt tttgaaaaaa
     2221 attgtggctg tatccagctc aaactggcat gataaggttc taacgacgaa tgttttaaat
     2281 acagatgaag acgtgaggca tcgccaaata aggaacgata actgacatca acaaaagctc
     2341 gaaattcatc ttctgtgcca taaccgatgc caaattctgt acttaaacgg gaagtctctt
     2401 cgacattaat tttcacagga ataggatttc ttttggttgt attatccagc agaggttgga
     2461 gagaaataat tctaaaaagc tgtaaatcaa ataaatgttt tcgtgtttta tcaattttct
     2521 ttttgttaaa aacctgtccg gaatcaaaag tacattggcg ataaatcaat tggtctttta
     2581 catatttatt accttcaatg gttgtatttc caaaaaaaca tttttcattt aatttaacgg
     2641 aaaaaatcac atctgcctta ctcaaatctg gttgtaaatg aatttgttgg tcacattctg
     2701 cataagcaaa acctttattt tgaaaaaaat ctttaataag attaatatca ttatagattt
     2761 tctgatctga aaaacgtttt cctgaaacag tgagcattct ttttctgaaa ggtaaaaaga
     2821 aagaatcttt ttttactttt aaactatctt caatttttat ctttacttca ccaaccagca
     2881 cgggcttgtt ttccacaatt ttaaaatata aatccacttt ttccttttta tcgttttgag
     2941 ccacggagtc aagttccacg ctggcattta aaaaaccttc gcattgataa aaacgaacta
     3001 accgatcaat gtctgattgg ataaaatttt cattataaat aaccggtttt tttttggaaa
     3061 gtactttgtc taaccatttt gtttcgtgca aggttgtttg ttttaatagt tcagatttgc
     3121 tgaaagactt gttaccgtta aatttgattt taccaatttc ataaccattt tgagctgaca
     3181 cctcaacaca atagacaaag aaaaggaaaa atacaagtcc ggaagaacag ttatatcgcg
     3241 taaaaatatt ttttttaaaa aaatcactca ttaacttttg taattgaaat ttttaaaaaa
     3301 tttgaacata tcacaaaaaa tacgtaaccc aaagaatgta aggttacttt ttgctattga
     3361 caaaattaga atttaatttc aaattttaag ccaaaattat caaaatgaat tatttgaatt
     3421 tacgatttaa tatcaaattg tattcaaaaa ggagaaataa atcgaaaata gtagggaatc
     3481 aataggtttg atgtgatgga tagggaatac atgaggcaag aaaacgagga tttttataga
     3541 gaatattgtt ttacgtcttg ataacttatc gtaagagaat atgatttatg ccaagacaat
     3601 tgaagcgaag cgaaatcacc agaatgttta aaattaaata tttcttgtaa caaaaagaaa
     3661 acgcagtctt gcacaaagct tcctattgaa aaacaacatt ttgtatattc cttgatgtca
     3721 acgtcaagtt atttcaaatc aaattttatt tttacaactc agtattaaat catgcagcct
     3781 tcgcaatagg ctaattttcc tctcaaacgc ttttcaatga gcaatcgcat ccttcctttc
     3841 agtgcttcga ttttgatgtt ttctatgaca tcattagtaa aagcaaacat caacaggaaa
     3901 cgagcttcag cttcagaaat gccacgactt tgcatgtaaa attttgccaa ttcatccaat
     3961 tgaccaatag ttgctccgtg agaacatttt acatcatctg cataaatttc caattgaggc
     4021 ttggatcgca ttctggcatt tttgctcagc aaaatatttc tgttgttttg ataagcaaaa
     4081 gttttttgag catgtggagc tacatatatt tttccactaa aggcaccttt tgaattttca
     4141 tcaagaatat atttgaataa ttcgttactt tgcgagttag gttttttatg aacaatagaa
     4201 gtaaaattat caactgtctg ggttttgtcg cctataacca ttcctgtcag taaagtttcg
     4261 caaaattcac catctatgtt tacttcagtg gaattccgtg tgataccatt atgcaaagtg
     4321 ataatattag cgagcacttt tgaagaagtc tgttgattaa ccaataaggt agaaacattg
     4381 gtcgtttgtt gatgtacatt ttccagatga taatgctcat aatgagcatt tttatcaaca
     4441 aaaatttccg tcaaacgatt cgagaaaaat ttcacgttat ccattgtatg atcgcaaacc
     4501 agcaattgag cttctgcacc ctcttcaatg ataattaaat tgcgtgtatt tgccattaaa
     4561 tcgacattgg aatgtagaat atttactagt tgcactggtt ttttcagttt aacatttttt
     4621 ggaatgtaca taaaaaaacc atcctgagca aacgtttcat taaatgcaat ccagccgtca
     4681 tttttttctc tgcttaattt accaatatat ttttctacca gttcagggta tttctctgaa
     4741 gctcccatta agctgtcaat gatcacacct tctggaaatt gctgattcga agtcgaagtc
     4801 acaggataaa aaacttcatt gactacaaaa tataaataag aacgtatgcc aggaacatcg
     4861 cacttaaata ctttataagg atttacagga attggcaagt gattgatatt taagccataa
     4921 tccactgtca aaggttcttt catatcagtg tatttgtaat cttcaagctt agcggtagga
     4981 aagcctaatt tttcaaattt agcaaaagct tcatctcgaa aagcattcaa caaaattgct
     5041 gaatgtgatt ttatatcttg ctgataactt ttataaaagt ctatatattg ttgtgccata
     5101 aggatttgta atattcaaaa catttttcgt tgtcaaatta aaaatctgat atttttcacg
     5161 tttaatattg aagttctttt tcttccaatt cttttttaat ccagtcgtat cctttttctt
     5221 caagttcggc tgccagtgtt ttatctcctg ttttcacaat gcgtccgtca taaaaaatat
     5281 gaacataatc aggaacaata taatccaata aacgttgata atgagtgatt acaataatag
     5341 aattatcagg acgtttcaat ttattcactc catttgccac aatgcgtaag gcgtcaatgt
     5401 ctaagcccga atctgtttcg tccagaattg ccagtcgtgg ttcgagcata gccatctgaa
     5461 aaatttcatt ccgcttcttt tcgcctcctg aaaaaccttc attaacagag cgatttgtta
     5521 atttattgtc catttccacc aattcttttt tttcacgcat caaacgcaga aaatcggcag
     5581 ccgaaagagg ttctaaattt ttatatttcc gatgttcatt taaagcagtg cgtaaaaaat
     5641 taaccatact tacgccagga atttctacag gattctgaaa acttaagaaa attccttcgt
     5701 gggaacgatc ttcgggcgaa atttccagta ggtttttccc atcaaaaata atttcacctt
     5761 tcgttacctc tactccagga taacctacaa tagctaatgc aagggttgat tttccacctc
     5821 catttggtcc cataatggca tgaacttcac ctgcttttac ggttaaattt acacctttta
     5881 gtatttcttt cccattaata tttgcataca aatttctaat ttcaagcata tcatttattt
     5941 tttcactcaa acaacaataa attgattttc aataaattta aaaaataatt ttcttctttt
     6001 caaccattca tatattatcc aattgaacct tcgagtgtta tttgcaataa tttttgtgct
     6061 tcaacggcaa attccatcgg taatttatta ataacttctt tggcataacc gtttacaatt
     6121 agcccaatgg cattttcggt agaaataccc cgctgattgc aataaaaaat ctgatcttca
     6181 ctgatttttg aagttgttgc ttcatgctct acaatagcac tttcgttgct cacatccata
     6241 taggggaaag tatgagcacc acatttgtca cccagcaata aactgtcgca ctgagaataa
     6301 tttcgtgcat tgtctgcatt ttttgacatt tgcaccagtc ctctgtagct attttgacca
     6361 taaccagccg aaattccttt tgaaagaata tgactgtgag tgtttttacc tatatgaatc
     6421 atttttgtgc ctgtgtcagc ttgctgataa tgattggtta cagcaacaga ataaaattca
     6481 gcactggaag aatccccttg caaaatacaa ctggggtatt tccatgtgat agcagagcct
     6541 gtttcaactt gtgcccacga aagttttgca ttttcttctt tgcaaatacc tcgttttgtt
     6601 a
//
LOCUS       DAOTKS010000032         6569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_11056_length_6569_cov_4.959932, whole genome shotgun sequence.
ACCESSION   DAOTKS010000032 DAOTKS010000000
VERSION     DAOTKS010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6569
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11056_length_6569_cov_4.959932"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            342..875
                     /locus_tag="P5236_01225"
     CDS             342..875
                     /locus_tag="P5236_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRU63016.1"
                     /translation="MDFFEKFQFCPACGSPFFIKNNEKSKRCQQCDFVFYMNASAAVA
                     AFILNEKNELLVCKRKKEPAKGTWDLPGGFVDNNETAEAAVKRELAEELHAETVQVEY
                     LFSEPNEYEYSDLVVPTLDLFFLCCLKDESQLIPSDDVEDCFYVALDKLEPDYFGLKS
                     IRKAIIRFIELTLGKAE"
     gene            950..3985
                     /locus_tag="P5236_01230"
     CDS             950..3985
                     /locus_tag="P5236_01230"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRU63017.1"
                     /translation="MKKSFFLFSFLCVITIPLLNAQYTLIYTDPNASFDKGKELYFQR
                     KYTASYHYFEDFLKTAKPQQAGQMHEAEYFLAANAFELRQKNAMELLNNFLLKYPYTS
                     YADPVYAMIGMLYYEQKNYSEAIIYFEKVKENNLNPDKRIDFLFCKGYAYLENKEYKK
                     ASDIFKDLKSVESPQKISAIYYYAYSQYLLGKYEEALPEFLKIKNESDYQAIVPYYLV
                     QIYYIQKDYKLARQEAFSLLENYPDNKNNAEIYRILGEIAYNEKNYKDAVFYLTKYEK
                     MFPQVLRNDIYILGLSYFQLKDYKNAVVYLSKVTTARDEISENAYLYLGSSYIYLNDK
                     INARLAYEAALETNFNPAVREEALFNYALTCYETPSAFGETISAFTRFLSEFPNSKYS
                     DEAYRYLAEAYLTTKNYELAYQSISQIKQLMPQLSETKQYLLYKLGSQAFVQNDYEKA
                     IDYFTRALEISSSNKYAAECLFWRAECFSRLQKSAQAQNDLTAFFKNAYAKSSVNFIM
                     AHYSMAYAYFSQKKYSEALNWFLKYINLESNSSSVTYVDALNRIGDCYYNARNFEQAL
                     KYYDKVVQLSPETADYALFQSAYIEGIKKNYTAKISKLEKLVTLYPGSEYNDNALYEI
                     ARSYLMLNNNEKAIATYQRLINQFSNSVLARKSMLEIGMIYLNEKNYDKAIASFKNVI
                     EKYPETEEFYTALESLETIYVEINDVNAYLNYAASLGQKIKNVAEQREDSISYIAAEK
                     QYLNGKYEVALTGLSSYVNKFCPGGRHCIIARYYLADCYYQTGEKEKALSTYQALLEL
                     PVSEFTEEAALRCAEITYDKKDYSSALNYFKKLETVAQSTINKNAARLGILRCNYFLN
                     DYQSLVNIASEIIADPRSDESLLIESLYYRAKSYLELGKNEQAIVDLKKLSVDTRTTW
                     GAESKYLLANVYFEQGKLKEAENEIMDFMKKNTPHQFWLAKSIVLLADIYVKKGDDFQ
                     AKQYLLSLRKNYLNHDEIENLIEERLQAIAKREKQKIIG"
     gene            4083..5780
                     /locus_tag="P5236_01235"
     CDS             4083..5780
                     /locus_tag="P5236_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63018.1"
                     /translation="MSVFGQDSIFRREVTVEREYVPVIREAGKINLTPKLYEPQVEKL
                     SPVYTEFYLPLPVETNIHPLQAIEFSLQREPLKNGYTKIGLGNYYNSLLDFAYPFIKK
                     EDMYLDFTVHHLSTYGNKWHTTSDASLFFNKDFKNLDFFTGVNASYERLKYYGNNFNL
                     DSIVDLKQLAGNYPLAIYLPTDKEQPIFSYSLKELSELNRFDTFWRGNIYGGVQSKSN
                     VTDIQYVGKLDYSFLNASSGSKEHQILLSGKFQIPYNDNKLGLDADLFQLNYNSKVTD
                     STNIENSYSVFIIHPYYDYHSDKFRARLGIQSAFSFEEKGFLHLYPDIQAEWQPFPTW
                     MAIYGGITGNHQVNSRSKILKENCFAEAYLKVNDTFTPFEGYLGIKLKPAELFFVDAF
                     IDYQHINNQYFFINKEFLSNDLREPNNHLFINRFDVTYSKTDLFKSGLRLSYHLQDRF
                     SCELKGVYNDWQVSDEQYAWNKPKWEADMNFQASLTAGVNLSAKIFYQGGRYAKLGEK
                     AISMKSAFDVNMGISYNYSRFLTVFAKVNNFFNSTYQQFYGYDVQGTNFLLGAALSF"
     gene            5828..6355
                     /gene="rimM"
                     /locus_tag="P5236_01240"
     CDS             5828..6355
                     /gene="rimM"
                     /locus_tag="P5236_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445025.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HRU63019.1"
                     /translation="MISKEEVFPIGEIIKPHGVKGEMSFLLKHPISDWEKIPFLIFSR
                     DGILVPFFIENIRWKSDNGGFLKLEDINTETAAHKFSGLTIFLHKKFLNHVISLEEEE
                     LQAEYFIGFQVINQENVLIGTIQEVDQSTENILFVVEKEGQEYLIPLSEEYIKNIDHK
                     KKEIFMELPEGLLDL"
BASE COUNT         2229 a          920 c         1115 g         2305 t
ORIGIN      
        1 aaaaattgtt cataacgatg aattatctca ttgatgtcat catcgttttg aaaaaatatt
       61 tttctgctca tgctttattt ttttgcaaaa ttaatatttt aattgtaata aaataaacct
      121 cttaactaaa agttttaaac aatcccgtat ttctttttga taaagataat atactgttaa
      181 aaggtttttt tcgttttacc aatacaaaca cttatggcat atttattgtt tactttgcac
      241 tattgtttgt cgtttttaat atcaattcaa gttaaatttt ttcatctgtt ggacaattac
      301 aagtcatttt gtttttaatt actttacttt gagacactta tatggatttt tttgaaaaat
      361 ttcagttttg tcctgcttgt ggttcacctt tttttataaa aaacaatgaa aaatcaaaac
      421 gttgtcagca atgtgatttt gttttttaca tgaacgcttc ggcagcagta gctgctttta
      481 ttttgaatga aaaaaacgaa ttactggttt gtaagcgaaa aaaagagcca gccaaaggta
      541 catgggattt gccaggtggt tttgtggata acaatgaaac tgctgaggca gccgtaaaac
      601 gggaacttgc ggaagaatta cacgcagaaa cggtccaggt ggaatatctt ttttcagaac
      661 ccaatgaata tgaatacagt gatttagtgg ttccaacttt agatttattc tttttgtgtt
      721 gtttaaaaga tgaatcccag cttattcctt ctgatgatgt ggaagattgt ttttatgtag
      781 cattagataa acttgagcca gattatttcg gcttgaaatc cataagaaaa gcaataattc
      841 ggtttattga acttactttg ggaaaagctg aatgaaatat ttaattttgc tttgtcaatg
      901 gtaaaaataa ttgtaatgaa atttatttaa atcaaaaatt gaagtgagaa tgaaaaaaag
      961 ttttttcctt ttcagttttt tgtgtgtgat aacaattcct ctgctgaatg ctcaatatac
     1021 tttgatttat actgatccta atgcttcttt cgacaaaggg aaagagcttt attttcaacg
     1081 aaaatatact gcatcttatc attattttga agattttctt aaaacagcaa aacctcagca
     1141 agcggggcag atgcatgaag cggaatattt tttagctgcc aatgctttcg agttgcggca
     1201 gaaaaacgca atggaattgt taaataattt tttgttgaaa tatccttata cttcttatgc
     1261 tgatcctgtc tatgccatga taggcatgct ttattatgaa cagaaaaatt atagcgaggc
     1321 aattatctat tttgaaaaag taaaagaaaa taatttgaat cctgataaaa gaatagattt
     1381 cttattctgt aaaggttatg cttatcttga aaacaaagag tataaaaaag caagtgatat
     1441 ttttaaggat ttaaaatctg ttgaatcgcc ccaaaaaatt tctgctattt attattatgc
     1501 ttattcacaa tatttgttag gaaagtatga agaagctttg cctgaatttt taaaaatcaa
     1561 aaatgaatct gattatcaag caattgttcc ttattatttg gttcaaattt attatattca
     1621 gaaagattat aagctggcaa gacaagaagc tttttcttta ttagaaaatt atcctgataa
     1681 caaaaataat gcagaaattt atcgaatact gggtgaaatt gcttataatg aaaaaaatta
     1741 taaggatgct gtattttatt taaccaaata tgaaaagatg tttcctcagg tgttaaggaa
     1801 cgatatatat attttaggtt tatcgtattt tcaattgaaa gattacaaaa atgctgttgt
     1861 ttatctgtca aaagttacta ctgcacgtga tgaaatttca gaaaatgctt atctttattt
     1921 aggaagttcc tatatttatt taaatgacaa aatcaatgct cgattggctt atgaagcagc
     1981 attagaaaca aacttcaatc ctgcggtgag agaggaagcc ttgtttaatt acgctttgac
     2041 gtgttatgaa actccttctg cctttgggga aactatttca gcttttactc gctttttatc
     2101 agaatttcca aactcaaaat attcagacga agcttatcgt tatcttgcgg aagcatatct
     2161 tactaccaaa aactatgaat tagcttatca gtccatttcc caaatcaagc aactaatgcc
     2221 tcaattatca gaaaccaaac aatatttgct ttataagctt ggaagtcaag cttttgttca
     2281 gaatgattat gaaaaagcaa tagattattt tactcgtgct ctggaaattt cttcttccaa
     2341 taaatatgct gctgaatgtt tgttctggcg agcagaatgt ttttcccgcc ttcaaaaatc
     2401 tgcccaagcc caaaatgatt tgactgcttt tttcaaaaat gcgtatgcaa aatcaagtgt
     2461 aaattttata atggcacatt attcaatggc ttatgcttat ttttcgcaga aaaaatattc
     2521 tgaagcgctg aattggttct taaaatatat aaatcttgaa tcaaattctt cttcagtgac
     2581 ttatgttgat gcattgaacc gaattggtga ttgctattat aatgctcgta attttgaaca
     2641 agctctaaaa tattacgaca aagtagttca actaagtcct gaaacagccg attatgcatt
     2701 atttcaatca gcttatattg aaggaattaa aaagaattac actgccaaaa tttcaaaatt
     2761 ggaaaaactg gtgacacttt atcctggttc tgaatataat gataatgctt tgtatgaaat
     2821 agcgcgttct tacttgatgc ttaataacaa tgaaaaagct atagctactt atcagcgatt
     2881 gataaatcaa ttctcaaatt cagttttggc tcggaaatca atgctggaaa taggcatgat
     2941 ttatttgaat gagaaaaact atgataaggc gatagcttct tttaaaaatg ttatagaaaa
     3001 atatcctgag actgaagaat tttatactgc tttagaaagt ttggaaacca tttatgtaga
     3061 aataaacgat gtgaatgctt atttgaatta tgccgctagc ttgggacaaa aaataaaaaa
     3121 tgttgccgaa caacgtgaag attctatttc ttatatagct gctgagaaac aatatttgaa
     3181 tggaaaatac gaagtagcat tgactggttt gtccagttat gtgaacaagt tttgtcctgg
     3241 aggacgacat tgtatcattg ccaggtatta tcttgctgat tgttattatc aaactggtga
     3301 aaaagaaaag gctctatcca cttatcaagc tttgctggag ttgcccgtaa gtgaatttac
     3361 agaagaagct gctttacgtt gtgctgaaat tacttatgat aaaaaagatt attcctctgc
     3421 tttgaattat tttaaaaagc tggaaacagt tgctcaatcg accattaata agaatgctgc
     3481 acgtttaggg attttacgat gtaattattt tttaaatgat tatcaatcgc tggtgaacat
     3541 tgcttctgaa ataattgccg atccacgttc tgatgagagt ttattaatcg aatcgcttta
     3601 ttatcgtgct aaaagttatc ttgaacttgg aaagaatgaa caggcaattg ttgatttgaa
     3661 aaaactttct gttgatacac gaacaacttg gggtgcagaa tcaaaatatt tactggcaaa
     3721 tgtgtatttt gaacaaggta aactgaaaga agcagaaaat gaaattatgg atttcatgaa
     3781 aaaaaatacg ccacatcaat tttggctagc aaagagtatc gtgcttttag ctgatattta
     3841 tgtgaaaaaa ggagatgatt ttcaggcaaa gcaatatttg ttaagcttac gtaaaaatta
     3901 tctgaatcat gatgaaattg aaaatcttat tgaggaacgt ttacaggcaa ttgccaaacg
     3961 agaaaagcag aaaatcattg gctaaattaa tttaagaaat tttgttcatc aattttagaa
     4021 aaattcttca aatgaataga aaaaaattat tgacctttat tttcatagtt gcaataaatt
     4081 tcatgtctgt ttttgggcaa gattctattt tcagacgtga agttacagtg gaacgggaat
     4141 atgtgcctgt cattagagaa gcaggaaaaa tcaatttaac tcctaaattg tatgagccac
     4201 aagtggaaaa attatcgcca gtttatacgg aattttattt gcctttacct gtggaaacca
     4261 acattcatcc tttgcaggca attgaatttt ctttacaacg agaacctcta aaaaacggct
     4321 atacaaaaat cggtttggga aattattata attctttgtt ggattttgcc tatccattta
     4381 taaaaaagga agatatgtat ttggatttta cagttcatca tctaagtacg tatggaaaca
     4441 aatggcatac tacttcggat gcgtcacttt tctttaataa agatttcaaa aatcttgatt
     4501 ttttcactgg tgtcaatgcc agctatgagc gattaaaata ttacgggaat aattttaatc
     4561 ttgattctat agttgattta aaacaattgg ccggaaatta tcctttggct atttatttac
     4621 caacggataa agaacaaccc attttttctt attctctgaa agaattgtct gaactgaatc
     4681 gatttgacac tttctggaga ggaaatattt atggaggtgt tcaatcaaaa tcgaatgtta
     4741 ctgatattca atatgttggc aaattagatt attctttttt aaatgccagt agcggaagca
     4801 aagaacatca gattttatta agtggcaaat tccagatacc ttacaatgac aataaattag
     4861 ggttggatgc tgatttgttt caattaaatt acaattcgaa agtaacggat tcaacgaata
     4921 tagaaaattc ctattcggtt tttatcattc atccttatta tgattatcac tccgataaat
     4981 ttagagctcg tttaggaata caatcagctt tcagttttga agaaaaaggt tttcttcatc
     5041 tttatcccga tatacaagcg gaatggcagc catttccaac atggatggca atttatggcg
     5101 gaataactgg aaatcatcaa gttaacagta ggagtaaaat tttaaaggaa aactgttttg
     5161 ctgaggctta tttaaaagta aatgatactt ttactccttt tgaaggttat cttggaatta
     5221 aattaaaacc tgctgaacta ttttttgtgg atgcttttat tgattatcaa catattaata
     5281 atcaatattt ttttatcaat aaagaatttc tctcaaatga tctgagagaa cccaataatc
     5341 atttgtttat caatcgtttt gatgtaactt acagtaaaac tgatcttttt aagagtggtt
     5401 tgcggctttc atatcatttg caggatcgtt tcagttgtga attgaaaggt gtttataatg
     5461 attggcaagt ttccgatgaa caatatgcct ggaacaaacc aaaatgggaa gctgatatga
     5521 actttcaggc ttcattgact gctggtgtaa atttatcagc taagattttt tatcaaggtg
     5581 gtcgttatgc aaaattaggt gaaaaagcaa tttcaatgaa atctgctttt gatgttaaca
     5641 tgggcatttc ttataattat tcccgttttt tgactgtttt tgcaaaagtg aacaactttt
     5701 tcaatagcac ttatcagcaa ttttacggat acgatgttca aggaacaaat tttttgctag
     5761 gagctgcatt gtctttctaa attagttaat ttttttaaca aataagagca tttcatatct
     5821 tttatttatg attagcaagg aagaagtttt tccaattggt gagattataa agcctcacgg
     5881 cgtaaaaggc gaaatgtcct ttttattaaa acatcccatt tcagattggg agaaaattcc
     5941 ttttttaatc tttagtaggg atggtatttt agttcctttt tttattgaga atattcgatg
     6001 gaaatctgac aacggtggtt ttctgaaatt ggaagacata aatacagaaa cagcagccca
     6061 taaattttct ggattgacta tctttttaca taaaaaattc ttaaatcatg tcatttcgtt
     6121 ggaagaagaa gaattgcaag cagaatattt tattggtttt caagtaataa atcaggaaaa
     6181 tgttttgatt ggaaccattc aggaagtgga tcaaagcacg gaaaatattt tgtttgtagt
     6241 tgaaaaagaa ggtcaggaat atttgattcc attaagcgaa gaatacatta aaaatattga
     6301 tcacaaaaaa aaggaaattt ttatggagtt gcctgaagga ttgttggatt tataaaatac
     6361 ctaaaatccg caagtcaatt attttgaaaa aagagggcat atttcagcgg acgttcaaat
     6421 tgccattttc aagatttcac gctgtttttt tctcattgtc taaaatcgtc cacaaaaatg
     6481 gggctcttat ttacaaattt aaggggagaa aacatgatat ttcatccttt ctttaacgtc
     6541 aagacatact ttaccctttc acgtttatt
//
LOCUS       DAOTKS010000033         6532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_11127_length_6532_cov_3.989347, whole genome shotgun sequence.
ACCESSION   DAOTKS010000033 DAOTKS010000000
VERSION     DAOTKS010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6532
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11127_length_6532_cov_3.989347"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(574..2628)
                     /gene="htpG"
                     /locus_tag="P5236_01245"
     CDS             complement(574..2628)
                     /gene="htpG"
                     /locus_tag="P5236_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HRU63020.1"
                     /translation="MAKGKIGVTSENIFPVIKKFLYSEHEIFLRELVANAVDATQKLK
                     TLTSIGEFKGELGDLTIRISVDKKKKTLTISDRGVGMTAEEIDNYINQIALSSAQEFL
                     EKYKNDAQSLIGHFGLGFYSAFMVAKKVEIFTLSYKEGATAQHWSCNGDPEYILEETI
                     KADRGTDVVLHIDEDNKEFLEENRIQELLNKYCKFLPIPIAFGKQKEWKDGKYVETDK
                     DNIINNTEPAWIKKPEELKEEDYLNFYHELFPMNEDPLFYIHLNVDYPFHLTGILYFP
                     RIKNNIDIQRNKIHLYCNQVYVTDHVENIVPEYLTLLHGVIDSPDIPLNVSRSYLQSD
                     ANVRKISNHITKKVADRLQEIFKNDRAQFEQKWEDLKLFIEYGMLTDEKFYERAEKFA
                     LFKNVDDKYFTFDEYKSLISDNQKDKNGNLIYLYATNANEQYSYIQQAKNKGYDVLLM
                     DGQLDMHLISQLENKWEKSRFVRVDSDVIDKLIQKEEIEKVPLTEGQQETLSIAFNSQ
                     LPAMDKTNFIIMFEHLKTNDSPVFITQNEFLRRMKQMSAIEGGMMQFYGEMPESFNLV
                     INTAHPLIDKILNEINSSCQEELEPIEAELKEKNEKFNQLKESHKGKKEEEIPAEEKD
                     ELENLQKELDELKEKKKTILTNFANQYKPIRQLIDLALLANNLLKGEPLANFVKRSFE
                     LL"
     gene            2876..5038
                     /gene="pnp"
                     /locus_tag="P5236_01250"
     CDS             2876..5038
                     /gene="pnp"
                     /locus_tag="P5236_01250"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRU63021.1"
                     /translation="MNTITKTITMADGREITIETGKLAKQADGAVMVRMGNTMLLATV
                     CAAEDAVEGTDFMPLSVDYKEKFAAFGRFPGGFLRREGRPSDYEVLIARLVDRALRPL
                     FPDDFHAETFVNIILCSADGEDMPDALAGLAASAALAVSDIPFKGPISEVRVARINGE
                     FVINPTFSQLEIADMDIIVAATMENIVMVEGEMKEVSEEDLLNAMKTAHQAIKIQCQA
                     QLELMKEVGKTEKRIYNHEENDEELKKDIWAKTYDKAYQIAVSGNNDKHSRTENFEAI
                     INDYIVTLDPEVAEAKKLLIDKYYHDVQKEAMRRAILDEGKRLDGRHTTEIRPIWCEV
                     DYLPGTHGSAIFTRGETQALVTVTLGTKMDEKLIDEPLIQGKERFLLHYNFPPFATGE
                     ARPSRGVGRREIGHGNLALRALKNILPEDYPYVIRVVSDILESNGSSSMATVCGGTLA
                     LMDAGVQIKKPVSGIAMGLISENKGEKYAILSDILGDEDHLGDMDFKVAGTKDGITAT
                     QMDIKIEGLSYEILEKALQQAKEGRLFILNKMLETIPEPRPDFKPHVPRIVIMFVPKE
                     MIGAIIGPAGKIIQNIQLETGATITIEEIGDQGRVEIAATNKEAIEAAVAKIKGIVAI
                     PEVGETYIAKVKSIMPYGIFVEFLPGKEGLLHISEIDWKHIDNIEQLGIKEGDMIEVK
                     LLDIDEKTGKFKLSHKALLPRPSRDERPPRKNNTMRNK"
     gene            5439..5840
                     /gene="rpsL"
                     /locus_tag="P5236_01255"
     CDS             5439..5840
                     /gene="rpsL"
                     /locus_tag="P5236_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRU63022.1"
                     /translation="MPTIQQLVRKGRSEIFKKSKSPALDACPQRRGVCVRVYTTTPKK
                     PNSALRKVARVRLTNQKEVNAYIPGEGHNLQEHSIVLVRGGRVKDLPGVRYHVIRGTL
                     DTAGVNGRLQRRSKYGTKRPKKSQTATAKNK"
     gene            5960..6436
                     /gene="rpsG"
                     /locus_tag="P5236_01260"
     CDS             5960..6436
                     /gene="rpsG"
                     /locus_tag="P5236_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018362998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRU63023.1"
                     /translation="MRKSKPKKRIVLPDPVYNDPMVTKFVNHLMYDGKKSIAFDIFYN
                     TLEIVKNKLPHEEKSSLEIWKKALENVTPLVEVKSRRIGGATFQVPTEIRPDRKESIS
                     MKNLILYARKRGGHSMSDKLAAEIVDAYNNQGGAFKRKEDIHRMAEANRAFAHFKF"
BASE COUNT         2244 a          994 c         1133 g         2161 t
ORIGIN      
        1 acaaaaatac gatatttatt tttaatattc ataattttat aaatataaaa ttatcagtag
       61 agtcagccag caagtgcgaa attaataaaa aatgttttaa aatcttgatt ttaaaacata
      121 aatgattgat atttcgccct tcaaaaagca agggtaaaaa gcatataaaa aagcaacata
      181 ttatttttaa tatttcacta aaaatgagca gattagttat atgattaaaa aaacaaaaaa
      241 tatgctactt gctaaagcta gaaaaaaatt ctgaaattga aatggatttc agtggaaaca
      301 gataacaaag tgtaaacatt ttgtgaggtt tcaaggcgtt gaaactgatg aaaaactttt
      361 caaaggtcaa gaatttgata ttaagttaca ttagggaata aaattaaccc cagaattttc
      421 atcagaaata aacattacgc ctcaaacaag aattatattg catataaaat tttgtacatt
      481 cttttagaat tgtctttaat gacaaatttg ggataaaaat aaagctcact ctactttttt
      541 cgtaaagtga gcttatagta aagctttctg cctttataat aattcaaagc tccgtttaac
      601 aaaatttgct agtggctcgc ctttgagtaa attgtttgcc aataaagcca gatcgatcaa
      661 ctgacgaata ggtttatatt gattggcaaa attagtcagg atagtttttt tcttttcttt
      721 caattcgtcg agctcttttt gcaagttttc caattcatct ttttcttcag caggaatttc
      781 ttcttctttt ttacctttgt gactttcttt taactgattg aatttttcat tcttttcttt
      841 caattctgcc tcgataggtt ccaattcttc ctggcaagag gaatttattt catttaagat
      901 tttatcaatc aaaggatgag ctgtgttgat aacaagattg aaactttcgg gcatttcgcc
      961 ataaaattgc atcatacctc cttctatagc cgacatttgc ttcatccttc gtaaaaattc
     1021 attttgcgta atgaaaacag gtgaatcatt tgtctttaaa tgttcaaaca taataatgaa
     1081 attagtttta tccatcgctg gcagttgtga attaaaagca atggaaagtg tttcttgttg
     1141 accctctgtc aaaggtactt tttcaatttc ttctttttga atcaatttat caattacatc
     1201 gctgtcaact ctaacaaatc ggctcttttc ccatttgttt tccaactgac tgatcaagtg
     1261 catatccagt tgaccatcca ttaataaaac atcgtaacct ttatttttgg cttgctgaat
     1321 gtaactgtat tgttcattcg catttgtggc atataaataa atcaaattcc catttttatc
     1381 tttttgatta tcagaaataa gtgatttata ttcatcaaag gtaaaatatt tgtcatccac
     1441 atttttgaac aaagcaaatt tttcagctct ttcgtaaaat ttttcatcgg tcaacatgcc
     1501 atactctata aagagtttca aatcttccca cttttgttca aactgagcac gatcattttt
     1561 gaagatttct tgaagtcggt cggctacttt tttggtaatg tggttagaaa ttttacgaac
     1621 atttgcatca ctttgtaaat aacttctgga aacattcaat ggaatatccg gtgaatcaat
     1681 tacgccgtga agcaatgtca gatattccgg aacaatattt tctacatgat cggttacata
     1741 aacctggttg cagtaaagat gaattttatt tcgttgaata tcaatgttat tcttgattct
     1801 tggaaaatac aaaattcctg tcaaatggaa cggataatcc acattgagat gaatgtaaaa
     1861 aagtggatcc tcattcatcg gaaacaattc atggtaaaag ttaagataat cttcttcttt
     1921 taactcttct ggtttcttaa tccaggcagg ttctgtatta ttaataatat tgtctttatc
     1981 ggtttctaca tatttaccgt ctttccattc tttctgtttc ccaaatgcaa taggaatggg
     2041 caaaaattta cagtatttgt ttaaaagttc ttgaattcta ttttcctcca aaaattcttt
     2101 attgtcttca tcaatatgca aaaccacatc agttcctcta tctgccttaa ttgtttcttc
     2161 aagaatatat tcaggatcgc cattacacga ccagtgttga gccgtagcac cttctttata
     2221 ggataaagtg aaaatttcaa ctttttttgc aaccataaat gctgaataga atcccaatcc
     2281 aaaatgtcca attagtgatt gagcatcatt tttatatttt tcaagaaatt cttgagcact
     2341 agataaagca atttgattga tataattgtc aatttcttcg gcagtcatcc ctacacctct
     2401 atcgctaata gtaagtgttt ttttcttttt atcaacagaa attctaatgg taagatcacc
     2461 taattcgcct ttgaattcac cgatagaagt aagggttttc agcttttgtg tcgcatcaac
     2521 agcatttgca accagttcac gaagaaaaat ttcatgttca ctgtataaga atttcttaat
     2581 tacggggaaa atattttcac tcgttacccc aatctttcct tttgccatat cttttgattt
     2641 taaaataaat tttacgaaat gagataatta ttttttgctt taaaattttt gcaaaaaaag
     2701 tgccattgag gatttggtga cagaatgtca gaatttaatt agtaatactt tcaaaaaagg
     2761 aaggtttttt attttagtta tgttgttatg aaattgacgg acattataaa aatattgtga
     2821 aaaattttgt aaatttgtgc cgaaaaaaat aatttaaaaa agaaaaggta attttatgaa
     2881 tacaattacg aaaaccatca caatggctga tggcagagaa ataacgatcg aaacaggtaa
     2941 gttagcaaag caagcagatg gagcggtgat ggttcgcatg ggaaatacga tgttattggc
     3001 aacagtttgt gctgctgaag atgcggttga aggcactgat tttatgcctc tttctgttga
     3061 ttacaaagaa aaatttgctg cttttggtag gtttccagga ggctttttac gccgtgaagg
     3121 tcgtccttcc gattatgaag ttttgattgc acgattggta gatcgagcac ttcgtccttt
     3181 gtttcccgat gattttcatg cagaaacttt tgttaatatt attctctgtt cagcagatgg
     3241 agaagatatg cctgatgcac tggctggtct ggctgcttcc gctgcacttg cagtttcgga
     3301 tattcctttt aaaggaccta tttcagaagt tcgtgtggca cgcattaatg gtgaatttgt
     3361 tattaatcct acttttagtc aacttgaaat agctgatatg gatattattg tagcagccac
     3421 aatggaaaat attgtcatgg tagaaggtga aatgaaagaa gtcagtgagg aagatttgtt
     3481 gaatgctatg aaaactgctc atcaggcgat aaaaatacaa tgtcaggctc agttagaatt
     3541 aatgaaagaa gttggtaaaa ctgaaaaacg aatatataat cacgaagaaa acgatgaaga
     3601 gcttaaaaaa gatatttggg ctaaaacgta tgacaaagct tatcaaatag ctgtttcggg
     3661 aaataatgac aaacattctc gcactgaaaa ttttgaagca attattaatg attatattgt
     3721 aacgcttgac cctgaagttg ctgaggctaa aaaacttttg attgataagt attatcacga
     3781 tgttcaaaaa gaagcgatgc gtcgtgcaat tcttgatgaa gggaaacgtc tggacggacg
     3841 tcatacaact gaaattcgtc ctatttggtg cgaagttgat tatttgcctg gcactcacgg
     3901 ttctgcgata ttcacacgcg gtgaaacgca ggcattagtc acagttacac ttggaaccaa
     3961 aatggatgaa aaattaattg atgaaccatt gattcaagga aaagaacgtt ttttgttgca
     4021 ttataatttt ccaccctttg ctacaggtga ggcacgtcct tcgcgcggag taggacgcag
     4081 agaaattggt cacggtaatt tagctcttcg tgctttgaaa aacatacttc cagaagatta
     4141 tccctatgtt attcgggttg tttctgatat tttggaatcc aatggttctt cttctatggc
     4201 tactgtttgt ggcggaaccc tggcattaat ggatgctggc gtccagatca aaaaacctgt
     4261 ttctggaatt gctatgggtt tgatttctga aaacaaggga gaaaaatatg caattttatc
     4321 tgatatatta ggcgatgagg atcatcttgg tgatatggat ttcaaagttg caggaacaaa
     4381 agatggcatt actgccacac aaatggatat caaaatagaa ggactttctt atgaaattct
     4441 tgaaaaagct cttcaacaag caaaagaagg aaggttattt atcttgaata aaatgctgga
     4501 aactattccc gaaccacgac cagatttcaa acctcatgtt cctcgtattg taatcatgtt
     4561 cgttcccaaa gaaatgattg gagctattat tggacctgct ggaaaaatca ttcaaaatat
     4621 tcaactcgag acaggtgcca ccataactat tgaagaaatt ggagatcaag gacgtgttga
     4681 aattgcagca accaacaaag aagctattga ggcagcagtt gccaaaatta aaggtattgt
     4741 agctatacct gaagtgggag aaacttacat agcaaaagtc aaatcaatta tgccgtatgg
     4801 tatttttgtt gaatttttac ctggcaagga gggtttgttg catatttcag aaattgattg
     4861 gaaacatatt gataacattg aacaattggg cattaaagaa ggagacatga ttgaagttaa
     4921 acttttagat atcgatgaaa aaactggaaa attcaagtta tctcataaag cattgttgcc
     4981 gcgtccttca agagatgaaa gaccaccaag aaaaaataac acaatgagaa ataaatgatt
     5041 ttttaattta tttatgtagt aaaagcctcg taaattgacg aggttttttt atcctatttt
     5101 aaagttgaaa taagtcaaga aaaattcttg taatttttaa ttccaaatac aaataaatat
     5161 tctttaataa aaattatgag aaatttagaa ttgaaattat tcaaagtatt attctgtatg
     5221 aataaataaa aatcaaatta ttatcacttt acgtgtattt ttttaattta cttttttatt
     5281 aataaaagct ataataaatt cataaaagga actttattga aaaaaatact tttaactcgt
     5341 tgtcaataaa aaaaaatatt cctaactttg caagccgttt tggtcaaatt gaagtaaata
     5401 gaaatcaact tgataaaaaa tttaaagtaa aataaaagat gcctaccatt caacagttag
     5461 ttagaaaagg aagaagtgaa atttttaaga aaagtaaatc acctgctttg gatgcgtgtc
     5521 cacaacgtag aggtgtatgt gtgcgtgttt atacaactac acctaaaaaa cctaattcag
     5581 ctttacgtaa agtagctcgt gtacgtttga cgaatcaaaa agaagtaaat gcttatattc
     5641 ctggcgaagg acataatttg caggaacatt caatagtatt agtgcgaggt ggacgtgtta
     5701 aagatcttcc aggtgttcgt tatcatgtca taagaggtac tttagatact gctggcgtaa
     5761 atggacgttt acagagacgt tcaaaatatg gtacaaaacg tcctaaaaag agtcaaactg
     5821 ctacggcaaa gaataaataa ataaaatatt tatagacatt tagataaaaa attgttgaag
     5881 agtttttatt tttaaaagtt gagtaaatga tcatttgatc gttgaagatt taaaaaacaa
     5941 ctaataagta aatttaaaaa tgagaaagtc aaaacccaaa aaaaggattg ttttacctga
     6001 tccagtttat aacgatccaa tggtaacaaa atttgtaaat catttaatgt acgatggcaa
     6061 aaagtcaatt gctttcgata ttttttataa tacacttgaa attgtaaaaa ataaattacc
     6121 ccacgaagaa aagtcatcat tggaaatttg gaaaaaagcc ttggaaaatg taacgcctct
     6181 ggtagaagtt aaatcaagac gtataggtgg cgcaaccttt caagttccta cagaaatccg
     6241 accagatcgg aaggaatcaa tttcaatgaa aaatttaatt ctatatgctc gtaaaagggg
     6301 aggtcattca atgtcagata agttggctgc agaaattgta gatgcttaca ataatcaagg
     6361 tggcgctttc aaacgtaagg aagacattca ccgtatggct gaagctaata gagcatttgc
     6421 acactttaag ttttaaaagt atttaaaaag aagacgagtt attaatggct aatgcggatt
     6481 taagctatac aagaaatatt ggcatcatgg cacacatcga tgccgggaaa ac
//
LOCUS       DAOTKS010000034         6403 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_11350_length_6403_cov_7.657372, whole genome shotgun sequence.
ACCESSION   DAOTKS010000034 DAOTKS010000000
VERSION     DAOTKS010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6403
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11350_length_6403_cov_7.657372"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1178
                     /locus_tag="P5236_01265"
     CDS             <1..1178
                     /locus_tag="P5236_01265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRU63024.1"
                     /translation="HEWQTAFGIFYLKQHMPQVATVFTTHATTVGRSIATNNKPLYDY
                     LNEYNGDQMSYELNVVSKHSAEKTAAHLADCFTTVSEITAKECAQLLEKKPDVVTPNG
                     FEDDFVPSKEKFQLKRQEARNILLKVSETLLGYKLKKDVLFVCTAGRYEFKNKGIDVF
                     IESLRRLLEHSNLNKEIVAFLMIPAHISGPRADLLACLKHSISKITSWNRFTTHELYD
                     YQNDNIMSAIRWFHFTNQASQKIKVIFVPSYLNGFDGIFNKSYYELLIGMDLTVFPSY
                     YEPWGYTPLESVAFSVPTITTDVSGFGRWVAQQPQGIENGVGIVHRSDYNQYEVSLNI
                     AEQVSHFSGYTEAQVEIIRKRAATIAEKALWEHFIQYYYEAYEVALTHRNDRLSQKS"
     gene            1429..4020
                     /gene="glgP"
                     /locus_tag="P5236_01270"
     CDS             1429..4020
                     /gene="glgP"
                     /locus_tag="P5236_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HRU63025.1"
                     /translation="MEIKMNSVNEPIWKDVHVKSYLPDGLKKLEELAYNICWVWKSDI
                     RHLFREIDIEAWQEAGFNPVQLLNNLSYDKLVETEKNSEFINKLDVLYNDFKNYMGRP
                     FSPDKPSVAYFSMEYGLTEVLKLYSGGLGILAGDYLKEASDYGVDLTAIGLLYRYGYF
                     TQSLSVNGEQIEIYDPQNFNLLPITQLKDKNNQPLLIEVPFPGRTVYANLWKIAIGRI
                     NLYLLDTDNELNSPEDKSITYQLYGGDWENRLKQEIMLGIGGILALKKLGIEKDVYHC
                     NEGHAALINVQRLIDLINDKNLSFYEALEVVRSSSLYTVHTPVPAGHDRFDEEMFSKY
                     MKMYAQKLKINWQDFMNLGREKPNDPNDKFSMSVFACYTCQEVNGVSWLHGKVSQEMF
                     SPLWPGYFPEELHVGYVTNGVHLPTWTTSEWKSVYEKYFGQEFYDDQSNVKIWQGIYE
                     IPDQVIWDSHLKMKKKLIEFIRTQFPEKWLKTQGDPSRIMTILNSLNPNALIIGFGRR
                     FATYKRAHLLFMDLGRLEKIVNDPQRPVQFIFAGKAHPADQGGKDLIKRIVEISRMPQ
                     FLGKIIFLENYDISMAKRLISGVDIWLNTPTRPLEASGTSGQKAQMNGVLNFSVLDGW
                     WIEGYVKDAGWAITEKRTYENEQYQDQLDAATIYSMLENEIIPLYFAKNAKGISPEWI
                     QYIKNSIALITPRFTTKRMIDNYIERFYLKLSNRLATLKKDNYTKAKELAAWKLKIAQ
                     GWDNIEVVSMDIPEKLFQNPQVSDFYQMNIVIDVKSINDEGIGIELVVTKMDQNQKYK
                     LYDVEEFKLVKADGSTLYYALDYRLNMSGTFKFGFRMFPKNIDLPHRQDLCYVRWLSY
                     DEFLLKT"
     gene            4102..4188
                     /locus_tag="P5236_01275"
     tRNA            4102..4188
                     /locus_tag="P5236_01275"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4136..4138,aa:Ser,seq:cga)
     gene            complement(4395..5723)
                     /locus_tag="P5236_01280"
     CDS             complement(4395..5723)
                     /locus_tag="P5236_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013816563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HRU63026.1"
                     /translation="MIGKSPRKDQRDLFRPMLEDFIDMNHELVLLANKIDWSYFEKEL
                     GVYYSANGAPSVPIRMMVGCMLLKHLYNLGDERLPEYWVRDVYFQYFCGCVFFEHRFP
                     FDPSDFVHFRNRIAEEGMEKIFAYSVKIHGKEVEKKSKLVLSDTTVQQNNTTFPTDAK
                     LCKKVIDKCNKIAEKEGIEQRRRYTRESKQLLRDTYNGTHPKRVKKAKKAKKRLKTIA
                     GRQIRELERKMNEEQKRRYEKELELYKRAVNQRKEDKDKIYSLHKPFTHCISKGKPHR
                     PYEFGNKVGLITTGKKGKKIITAIRAFTGNPFDGHTIDPLLEQMEGNNLKLPEELVYD
                     RGGRGKKEIRGVKIITPGKPKASDTAYQKQRKRRKFRARSGIEPIIGHLKKDFRMEQN
                     YLMGEEGIQINAFLAASAWNLKKMMEKLKEEFFCFIFRLLFRQNFYNLAT"
     gene            complement(6209..>6403)
                     /locus_tag="P5236_01285"
     CDS             complement(6209..>6403)
                     /locus_tag="P5236_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63027.1"
                     /translation="LEDVMNFIKNQGEDEKKTESNLEEDQKSNEISEEPSEDDPVSDE
                     LDKENNDPKSEKNFFDKFKF"
BASE COUNT         2054 a         1056 c         1075 g         2218 t
ORIGIN      
        1 ttcacgaatg gcagactgct tttgggattt tttatttgaa acagcatatg ccacaggttg
       61 ctactgtttt tactactcat gccacaaccg ttggacgttc catagcaaca aacaataaac
      121 ctttgtacga ttatttgaat gagtataatg gtgatcaaat gtcttatgaa ttaaatgtgg
      181 tctcaaaaca ttcagcagaa aaaactgctg ctcatctggc agactgtttt accacagtaa
      241 gtgaaattac tgcaaaggaa tgtgctcagt tgctcgaaaa aaaacctgat gtagtcaccc
      301 ctaacggctt tgaagatgat tttgttcctt caaaagaaaa gtttcaatta aaaagacaag
      361 aagctcgcaa tattttatta aaagtatccg aaactttatt aggctataag ttaaagaaag
      421 atgttttatt tgtttgtact gcaggacgtt atgaatttaa aaacaaagga attgatgttt
      481 ttattgaatc attaagacgt ttgttagaac attcaaattt aaataaagag attgtagctt
      541 tccttatgat tcctgctcat atttcaggtc ctcgtgcaga tttacttgca tgcttaaaac
      601 attcaatttc taaaattact tcttggaacc gatttacgac tcatgaatta tatgattatc
      661 aaaatgataa tataatgtcc gccatccggt ggtttcattt tacaaatcag gcttctcaaa
      721 aaataaaagt tatttttgtg ccttcttatt tgaatggctt cgacggtatt tttaataaat
      781 cctattatga attactgata gggatggacc ttacagtttt tccatcatat tatgaaccct
      841 ggggttatac tcctctggaa agtgttgctt tttctgttcc tacaattact actgatgttt
      901 caggttttgg acgatgggtt gctcaacaac cccagggaat tgaaaacggg gtaggaattg
      961 ttcatcgttc cgattataat cagtatgaag taagccttaa tattgcagaa caagtatctc
     1021 atttttcagg ttatacagaa gcacaagttg aaattattag aaaaagagct gctactattg
     1081 ctgaaaaagc tttatgggaa cattttattc aatattacta tgaagcttat gaagtagctc
     1141 ttacacatag aaatgatcgc ttatcccaaa aaagttaata agataatcta ttcttctaac
     1201 aataaatgat aaaattagaa ttttttctta agatatatcg ataatttgat tataaaaata
     1261 ttttgaaatg atttttaatc gttacttttt attagtaatg taaatgtttg taataaatat
     1321 ttgctttttt tgttatattt tatttaggag tttattactc ttctattatt tttgacaaaa
     1381 aaataatttt ttgttattta aaaattcaat tggaaggaaa aattgattat ggaaattaaa
     1441 atgaattctg ttaacgaacc gatatggaaa gatgttcatg ttaaatctta tcttcctgat
     1501 ggtttaaaaa aattagaaga attagcttac aatatatgct gggtatggaa aagcgatata
     1561 cgtcacctat ttcgtgaaat agacattgaa gcttggcaag aggcaggatt taatcctgtt
     1621 caattactaa ataacttaag ttatgataaa ttggttgaaa cagaaaaaaa ttctgaattt
     1681 ataaacaagc ttgatgttct ttataatgat tttaaaaatt atatgggacg accattttca
     1741 ccagataagc ccagcgtagc ttatttcagt atggaatacg gtttaactga agttttaaaa
     1801 ctctattcag gaggattggg aatattagca ggagattact taaaagaagc aagtgactat
     1861 ggagttgatt tgacagccat tggtttatta tatcgttatg gttattttac gcaatcgctt
     1921 tccgttaatg gtgaacaaat tgaaatttat gatccacaaa actttaattt attaccaata
     1981 actcaattga aggataaaaa taatcagcct ttgttaattg aggttccttt cccaggacga
     2041 actgtatatg caaatttatg gaaaatagcc ataggacgaa taaatttata tttattagat
     2101 actgataatg aacttaatag ccctgaggat aaaagtatca cttatcaact ttatggtggt
     2161 gattgggaaa ataggttaaa acaggaaatc atgctgggca ttggaggaat tcttgcttta
     2221 aaaaaattgg gcattgaaaa agacgtctat cattgtaacg aaggacatgc agctttaatc
     2281 aatgttcaac gtctaattga tttgatcaat gacaaaaatc tttccttcta tgaagctctt
     2341 gaagtagtta gaagcagttc tttgtatact gtacatacac ctgtacctgc aggtcacgat
     2401 cgctttgatg aagaaatgtt ttccaaatac atgaaaatgt atgcacaaaa attaaagatc
     2461 aactggcaag attttatgaa tttaggacgt gaaaaaccta atgatcctaa tgataaattt
     2521 agtatgagtg tgtttgcatg ttacacttgc caggaagtaa atggtgtaag ttggttgcat
     2581 gggaaagttt ctcaagaaat gtttagtcct ctctggccag ggtatttccc agaagaatta
     2641 catgtaggtt atgtaacaaa cggtgtacat ttgcctacgt ggaccacttc agaatggaaa
     2701 tcggtatatg aaaaatattt tggacaagag ttttatgatg atcaatcgaa tgtaaaaata
     2761 tggcagggaa tatatgaaat ccctgatcag gtgatctggg attctcattt aaagatgaaa
     2821 aagaaattga ttgagtttat tcgcacccaa ttccctgaaa aatggttaaa aacacaaggt
     2881 gatccttctc gaattatgac aatattgaat tcactgaatc caaatgcctt gattattggc
     2941 tttggacgac gatttgcaac ttataaaaga gctcatttgc tttttatgga tttgggacgg
     3001 ttagaaaaaa ttgttaatga ccctcaacgt cccgttcaat ttatttttgc tgggaaagct
     3061 catcctgcgg atcaaggtgg aaaggactta attaaaagaa ttgttgaaat ttcaagaatg
     3121 cctcaatttt tagggaaaat tatttttctc gaaaattatg atatctccat ggcaaaacgc
     3181 ttgatttcgg gagttgatat ctggctaaat acccctacca gaccgttaga agcttctgga
     3241 acttcaggac aaaaggcaca aatgaatggt gtccttaatt tctctgtttt ggatggctgg
     3301 tggattgaag gctacgtaaa agatgctggt tgggctatta ctgaaaaacg tacttatgaa
     3361 aatgaacaat atcaagatca actggacgct gctactattt atagcatgct ggaaaatgaa
     3421 attatacctt tgtattttgc caaaaatgca aaaggaattt cgccagaatg gattcaatat
     3481 attaaaaact ccattgctct catcacacca cgttttacta ccaaacgaat gattgataat
     3541 tatattgaac gtttttattt aaaattgagc aatcgtttgg caacgttgaa aaaagataac
     3601 tatacaaaag caaaagaact ggcagcatgg aaattaaaaa ttgctcaagg ctgggataat
     3661 attgaagttg tatcaatgga tatacctgaa aaattatttc aaaatcctca agtcagcgat
     3721 ttttatcaaa tgaatatcgt aattgatgta aaaagtatca atgatgaagg aattggcatt
     3781 gaattggtag taacaaaaat ggatcaaaat caaaaatata aattgtatga tgtggaagaa
     3841 ttcaaattag tcaaagcaga cggttctacc ttgtattatg cacttgatta tcgtttaaat
     3901 atgtcaggaa cttttaaatt tggttttcgg atgtttccaa aaaatataga tcttccacac
     3961 cgtcaggatc tatgttatgt tcgctggtta tcatacgatg agttccttct taaaacttaa
     4021 ttttttaatc aaaagaattc ctcgaggctt gcctcggggt tattcattca taaagtttta
     4081 gaagaaatcc atgttaaaaa aggaaagttg ccggagtggt cgatcgggac ggtctcgaaa
     4141 accgttgtac gggtaactgt accgagagtt cgaatctctc actttccgct tttttgtagg
     4201 cagatatttc tcctatgtta cttatccaca cgttaagata gtgaattttt ttaattccaa
     4261 aaagtttttt tcgttgagta tttcacaaaa tattttatct ttatagttgc caacaagacc
     4321 aactgctatc ataatgtcct ttaaattaaa aaattaaatg taaattaatt agtcattcct
     4381 taaaaagtct atttttaagt ggcaagatta taaaagtttt ggcgaaaaag taatcgaaaa
     4441 atgaaacaaa aaaactcttc tttgagtttt tccatcattt tcttcaaatt ccaagctgat
     4501 gcagccaaga aagcgtttat ttgtatgcct tcttcgccca tgaggtaatt ctgttccatt
     4561 cggaagtctt ttttcagatg tccgataatt ggttcaatgc ctgatcttgc tctaaacttt
     4621 ctacgtttcc gctgcttttg ataagctgta tcacttgctt tgggtttacc tggcgttatg
     4681 atttttactc cccgtatctc tttctttcct cgtccgcctc tatcataaac caattcctcc
     4741 ggcagtttta aattgtttcc ttccatttgt tccagtaaag gatctatcgt atgtccatca
     4801 aatggatttc ctgtaaatgc ccttatggca gttatgattt tcttcccctt ctttcctgtc
     4861 gttatcaatc ctaccttgtt cccaaactca taaggcctgt gcggcttacc tttggaaata
     4921 caatgagtaa atggtttgtg taaactgtaa atcttatcct tgtcctcttt cctttgattc
     4981 actgctcgtt tgtataattc cagctccttt tcgtatcttc ttttctgttc ttcattcatc
     5041 tttctttcca attctcgtat ctgcctacca gctatcgtct tgagacgttt ctttgctttc
     5101 ttcgctttct tgacacgctt gggatgagta ccgttgtacg tatctcgtag taattgtttg
     5161 ctttccctcg tgtaacgacg acgttgttct atcccttctt tttctgctat cttattgcat
     5221 ttgtctatca ctttcttgca caatttggca tctgttggaa aagtagtatt gttttgctga
     5281 acggtcgtat cagataaaac taattttgat tttttctcta cttctttccc atggatcttt
     5341 acactgtaag caaaaatctt ttccatccct tcctctgcga ttcgattacg aaaatgtaca
     5401 aaatcactcg gatcaaaggg aaagcggtgc tcaaaaaata cacacccgca aaaatactga
     5461 aagtaaacat cccgtaccca atactccggc aatcgctcat ctcccaaatt gtacaaatgc
     5521 ttgagcaaca tgcagcctac catcatccgt atgggaacgc ttggtgctcc gttggcagaa
     5581 taataaacgc ccaactcctt ctcaaaataa ctccaatcta ttttgttcgc taataaaacc
     5641 aattcgtggt tcatatcaat aaagtcttcc aacatgggac gaaataaatc tcgttgatct
     5701 tttctcggtg attttcctat cataatttgc agtattttat actgtaaaga tactcattcc
     5761 tgcaaatata aacaaacttt tttacaacta ttttattgat atttaatcga atagaataaa
     5821 tttaaggaac gactaactac tttctaattt ttttcttctc acttttgtgt tctccaaaag
     5881 aaagaaccat gtctaatctc tctttatcta tcgtcacata tatgaaacgt tcattttaca
     5941 ttcctcccat tgagaaggac ttttataaca atttctcaaa attctgaaag aaaaatccgt
     6001 tatatggaaa gttattttga gaaaggaatt gattggatcc cagaatatta tgaaaagagt
     6061 gtttgcaaaa caaataaatt tatagagaga taagtgctta ttatggagat agtgaaaata
     6121 ctgttaagat aaaaaacact gctattcatc tttttgaaca gcagtgtatt aagagaataa
     6181 aaacagacat tggtagagaa gtgagaattt aaaatttgaa tttatcaaaa aaatttttct
     6241 cggatttggg atcattattt tctttatcca actcatcact cacaggatca tcttcacttg
     6301 gctcttcgct tatttcattg gatttttgat cttcttcaag gttactttct gtttttttct
     6361 cgtcttcacc ctgattctta ataaaattca ttacatcctc taa
//
LOCUS       DAOTKS010000035         6376 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_11403_length_6376_cov_4.628856, whole genome shotgun sequence.
ACCESSION   DAOTKS010000035 DAOTKS010000000
VERSION     DAOTKS010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6376
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11403_length_6376_cov_4.628856"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..3845)
                     /locus_tag="P5236_01290"
     CDS             complement(192..3845)
                     /locus_tag="P5236_01290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRU63028.1"
                     /translation="MNTITMHKNAEMKLKKTNIVLLFILLLSLAGGMICEYLYRNSPK
                     NAIQFNQFQNQLISKELKASRTLKMMKQLIENNSFDSLIKLPFPNNDISFYVFRDNEL
                     IFWSDHNLEINNIPLSDSTNLHFVQLPNAYCVSQSLNFDHYKLLSLIKIKNNFPSENQ
                     LLVNNFAKGFEMDPRVGITIGSNLDQYAVFSTHGDYLFSLVPPSQPIYNENFAKLGLI
                     FFLVFFLCFFIFYARFPSLLHKKNIDIKTFVVAFLIIGLFVFAGIYFNFPETLYANHL
                     FSPFQYAAHPFLSSIVHLVIITGYFIASICLFYFYIKINRKVSRLTTFLLQLTLPLCF
                     LLLYFLLKGIVFHSGEQVNILNFKDFSPFTITIHILILGWGIGFGLLFYKIHRWIKKS
                     HLLKIALINELFFILCMGLITYFISRTDFKQVTISYILLCTPLYFFIFSKKRKNIYFQ
                     IGIYLFVFTVFFIGNSFLFQQEKKLNKYKTLAQNIFINGTAENDPMTEILLQELDTQL
                     INDTTFSFLLQNEDSLQSASKYISNTYLRGFWNRYEIHLNTATATSALYKEYQSFVEE
                     NGTPIKQTHFFNIQPNEKNISYLGIFPVSSLKSDSLFLFMEFYPRQHVRSYSFPNLLI
                     SSAPDISTQYNISIARYENRKLVYSTGLVNFPNNSSWLPYKISEFYSFQQNYRTFYVY
                     TPTRTTYLVITDNQPIVFSSYLFYFLYTFLSYYFIAWLMLQIYLYLKKGGKYSLGFTA
                     KFQLSFIVLLLLSFFGIFYVSVNFLQEKYRKEQIQNLETKKNYIQKALQDKYYWTQEL
                     NEKNVKILNFDLQDLSYTYQIDIHVYNNDGILMASSQPLIFNKGLISNRIAPQPYFSE
                     NPNISQYESIGKLNYLVAYTDFYNGDYLQIGYIAVPQFFSQDEIKNEIESFMSVIIQI
                     YLVIIILVILLTLLIGKQLSTPLRMLENKLKEMQIGKRNDKIDYKLNDEIGHLVTQYN
                     LTVDQLEESARLLARSERESAWKSMARQIAHEINNPLTPMKLTIQQLKRTKQIDKEQF
                     DNYFAKATEMLIEQIDNLSHIARTFSEFARMPDAKFEYLDIAQLLHSVVQLFSNNHGH
                     IKISFLGKENGIYVYADPNQMMEVFNNLLKNALEAIPENREGKIIVEITEMDQKITIK
                     ISDNGVGIPDEMKNKLFVPNFTTKNAGMGLGLAISKNIIDLTGGSITYKTKLNEGTTF
                     IISLPKGKNDLFT"
     gene            complement(3823..4296)
                     /gene="rlmH"
                     /locus_tag="P5236_01295"
     CDS             complement(3823..4296)
                     /gene="rlmH"
                     /locus_tag="P5236_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007133455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HRU63029.1"
                     /translation="MKFILLTIGGTTNKHLLHLQEDYKNRIKFYLPFEIITLPEIKNT
                     KKLSVPEIVEKEGELIFNYLQITDEVILLDEKGEQFTSVGFSEFISKKLLSSKKRIIF
                     VVGGSYGFAKEIYPKANHTIALSSMTFSHEMVRVIFLEQLYRAMTILKGEHYHHA"
     gene            4586..5020
                     /locus_tag="P5236_01300"
     CDS             4586..5020
                     /locus_tag="P5236_01300"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HRU63030.1"
                     /translation="MRILKLLFICSILFIPNVLLSQTTVIPSDIISSFNNGDASRLSN
                     YLNDNVELVLDNNNNVYSKQQTLTILTDFFKKNKVNSFQVLHQGEKEASNFVIGTLKT
                     SNGNFRVYILMRKTNNKTLILQLRIEPSNESRLQGTDSFMAF"
     gene            4977..5822
                     /gene="nadC"
                     /locus_tag="P5236_01305"
     CDS             4977..5822
                     /gene="nadC"
                     /locus_tag="P5236_01305"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HRU63031.1"
                     /translation="MNQDYKELIPLWLSEDIGDGDHTTLACIPADARGKSRLIIKENG
                     VVAGIEVAKEIFHTFDPELSMTKFINDGDEVKIGDIAFEVKGKIQSLLQTERLMLNVM
                     QRMSGIATKTRQYVKALEGTKTKVLDTRKTTPGLRALEKEAVRIGGGVNHRFGLYDMI
                     LLKDNHIDFAGGIKEAIFRTKEYLKNKGKNLKIEIEVRNLQELSEVLSIGGVDWIMLD
                     NFNIENTQKAVEMVAGRYMIESSGGITLETLRSYAECGVDFISVGALTHSVKSLDMSF
                     KAVNF"
     gene            5915..6295
                     /locus_tag="P5236_01310"
     CDS             5915..6295
                     /locus_tag="P5236_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63032.1"
                     /translation="MLKQYLNSLQTTFNQGDAREESYYEHLKDLLLNYANERQLKKVD
                     ITILPKPTEAGNPDFRIWDGKNHITGYIEAKDPSVTNLDRIEVSEQLKRYRETFPNII
                     LSCDLLSNFISLIFGTTHLYICEN"
BASE COUNT         2338 a          851 c         1036 g         2151 t
ORIGIN      
        1 aacatctaac gaaagtaaac gtgctggttt tatggtagtt gtggttgtca tatttcagaa
       61 attttttaaa aaacttttac ttaatggaca aaaaataaaa ggtaaaaatc ggagaaatgg
      121 aattttcatt ttcttttaaa aactcaggtc acaaatttaa aagaaaaaaa cgaaacttgt
      181 ttatagaaaa attatgtaaa taaatcattt ttcccttttg gtaaagaaat aataaatgtg
      241 gtgccttcat ttaacttcgt tttataagta atacttccgc ctgtcaagtc tataatattt
      301 tttgaaattg ccaatccaag tcccatgccg gcatttttag tagtaaagtt cggaacaaac
      361 aatttgtttt tcatttcatc aggaatgcct actccattat ctgatatttt aattgtgatt
      421 ttttgatcca tttcagtaat ctctacgata attttccctt ccctgttttc agggattgcc
      481 tccaaagcat ttttcaataa attattaaac acttccatca tttgatttgg gtctgcatat
      541 acatagatgc cattttcttt acctaaaaaa gaaattttta tatgaccatg attattacta
      601 aaaagttgaa cgacagaatg taataactga gcaatgtcta aatattcaaa tttagcatcg
      661 ggcattcgag caaactctga aaaagtacgt gcaatatgag acaaattgtc aatttgctca
      721 attaacattt cagttgcttt tgcaaaataa ttatcaaatt gttctttatc aatttgttta
      781 gtacgtttca gttgctgaat agtcaatttc attggtgtta agggattgtt aatttcgtga
      841 gcaatttgcc ttgccattga tttccatgcc gattctcttt ctgaacgtgc caaaagtcga
      901 gcactttctt ccagttgatc tacggtgaga ttatattgag ttactaagtg accaatttca
      961 tcatttaatt tataatcaat tttatcattg cgtttaccaa tttgcatctc ttttaatttg
     1021 ttttcaagca tgcgaagagg agtagaaagc tgttttccaa tcaataaagt taataaaata
     1081 accagtataa taataacgag ataaatttga ataatcacgg acatgaaact ttcaatttca
     1141 tttttaattt catcttgact gaaaaattgt ggtacagcaa tatagccaat ttgcaaataa
     1201 tcaccattat aaaagtcagt gtaggcgaca agataattta attttccaat tgattcgtat
     1261 tgactgatat tgggattttc tgaaaaataa ggttgtggag caattcgatt actaatcagc
     1321 cctttattaa aaattaaggg ttgtgagctt gccattaata ttccatcatt gttatataca
     1381 tgtatgtcaa tttgataagt atatgaaaga tcttgcaaat caaaattcaa aattttaaca
     1441 ttcttttcat ttaactcttg tgtccaataa tatttgtcct gcaaggcttt ttgaatataa
     1501 ttttttttgg tttccagatt ttgtatttgt tcttttcgat atttttcctg gagaaaattt
     1561 actgaaacat aaaaaattcc gaaaaaacta agaagtaaaa gaacgataaa cgaaagttga
     1621 aattttgcag taaatccaag actatatttc cctccttttt taagatataa ataaatttgc
     1681 aacatcagcc atgcaataaa ataataggac aaaaaggtat aaagaaaata aaacagataa
     1741 gaagaaaata caataggctg attatcagtg attaccaaat aagtagtgcg tgttggcgta
     1801 tagacataaa aagttcgata attctgttga aaactgtaaa attctgaaat tttgtaagga
     1861 agccaggaag aattatttgg aaaatttacc aaccctgttg agtagactaa ttttcgattt
     1921 tcatatcgag caatagaaat attgtattga gtagaaatat caggtgcaga acttattaat
     1981 aaatttggaa aactataact gcgtacatgt tggcgtggat aaaattccat aaaaagaaac
     2041 aaagaatcac tttttagaga agatacagga aaaataccta aataagaaat gtttttttca
     2101 ttgggttgaa tattaaaaaa atgagtttgt ttaataggtg tgccattttc ttcaacaaaa
     2161 gattgatatt ctttgtataa agcagaggtt gcggtcgctg tatttaaatg tatttcatat
     2221 ctgttccaaa aaccacgtaa ataagtattg ctaatgtatt tgcttgctga ttgaagagaa
     2281 tcttcattct gtaataaaaa agaaaaagta gtatcattaa ttagttgggt atctaattct
     2341 tggagcagga tttcagtcat tgggtcattt tcagcagtgc cattaataaa aatattttga
     2401 gccagagttt tatatttatt cagttttttt tcttgctgaa aaaggaaaga atttcctatg
     2461 aaaaaaacag taaaaacaaa taaatatatt cctatttgaa aataaatatt tttccttttt
     2521 tttgaaaaaa taaagaaata caaaggtgta cataataata tataggaaat tgttacttgc
     2581 ttgaaatcag ttcgtgaaat aaaataagta atcaaaccca tgcaaagaat aaaaaataat
     2641 tcatttataa gtgcaatttt caataagtga gattttttta tccagcgatg tattttgtaa
     2701 aataataatc caaaaccaat tccccacccc agtatgagaa tatgaatggt aattgtaaaa
     2761 ggagaaaaat ccttgaaatt caaaatatta acttgttcac cagaatgaaa aacaatccct
     2821 ttcagcaaaa aatataacaa aagaaaacat aatggcagtg tcagttgaag taagaacgtt
     2881 gtaagtcttg aaacttttct gttaattttt atatagaaat aaaacaagca aatactagca
     2941 ataaaatagc ctgtaatgat aactaaatga acgatagagc ttaaaaaagg atgggctgca
     3001 tattggaaag ggctgaaaag atgattggca tataaggttt caggaaaatt gaaataaata
     3061 cctgcaaaaa caaacaaacc aataattaga aaagcaacaa caaaagtttt aatatcgata
     3121 ttttttttat gtaaaagcga tgggaaacgg gcataaaaaa taaaaaaaca taagaaaaac
     3181 acaaggaaaa aaattagtcc taattttgcg aaattttcat tgtaaatggg ttgtgaaggt
     3241 ggaaccaaag aaaaaagata atcaccatgc gtgctgaaca ctgcatattg atctaaattg
     3301 gaaccgattg tgatacctac tcggggatcc atttcaaaac cttttgcaaa attgttaact
     3361 aacaactgat tttcagaagg aaaattattt ttgattttaa tgagcgataa aagtttgtag
     3421 tggtcaaagt ttaatgattg actaacacaa taagcattgg gtaattgaac aaaatgtaaa
     3481 ttggtagaat cagatagagg aatgttgttt atttccagat tatgatctga ccaaaaaatc
     3541 agttcattat cacgaaaaac ataaaatgaa atatcattat tgggaaatgg aagtttgatt
     3601 agtgaatcaa aagagttgtt ttcaataagt tgcttcatca tttttaaggt gcgtgaagct
     3661 tttaattcct tgctaataag ttgattttga aattgattaa attggatagc attttttgga
     3721 gaatttcgat ataaatactc gcaaatcatt cctcctgcca aagataatag cagaataaac
     3781 agtaaaacaa tatttgtttt tttcaacttc atttcagcgt ttttatgcat ggtgatagtg
     3841 ttcacctttt aaaatggtca ttgctcgata aagttgttca agaaaaataa ctcgcaccat
     3901 ttcatgagaa aaggtcatag aagaaagagc aatagtgtga ttggcttttg ggtaaatttc
     3961 ctttgcaaaa ccatacgagc cacctacaac aaaaattatg cgcttttttg aagacaaaag
     4021 ttttttagaa ataaattctg aaaaaccaac agaagtaaat tgttcacctt tttcgtctaa
     4081 taatattact tcgtcagtga tttgcaaata attaaaaatt aactctccct ctttttcaac
     4141 tatctcagga acagataatt tcttagtatt ttttatttct ggcaatgtta ttatttcaaa
     4201 cggaagataa aattttatcc tgtttttata atcttcctgc aaatgtaaaa gatgcttgtt
     4261 agtagttcct ccaattgtta aaagtataaa tttcatactg agatagttgc tgtttttttg
     4321 gctcgttctt tgatgtgtat ttttagtaca aatatatgaa aactctttaa gaatgaaaaa
     4381 gttaaattag tgtgtcttta cgttttaaag tagaaataag gtaggagaag aaatgttttt
     4441 cagttataaa ttgcaattta ttataaattt atgaaatgaa tgataaaata agatttatct
     4501 ttgtatgtgc tttcgggtat tttttgagat tggtctttta agggataaat cattaattaa
     4561 ttaatttatt aaaaattttg agaacatgag aatcctaaaa ttacttttta tttgcagtat
     4621 attgtttatt cccaatgtgt tgctatctca aacaactgtt attccttctg atatcatttc
     4681 gtcatttaac aatggcgatg ccagtcgatt gtcaaattat ttaaatgata atgttgaact
     4741 ggtattggac aacaacaata atgtttatag caagcaacaa acattgacta ttcttactga
     4801 ttttttcaaa aaaaataaag taaatagttt tcaagttctt catcaaggtg agaaagaagc
     4861 ttctaatttt gttatcggta ctctcaaaac ctcaaatgga aatttcagag tctatatttt
     4921 aatgagaaag acaaacaata aaacattaat tttacaatta agaattgaac caagcaatga
     4981 atcaagatta caaggaactg attcctttat ggctttctga agatattggc gatggtgacc
     5041 atactacttt agcatgcatt cctgccgacg caagaggaaa gtcaagattg attattaaag
     5101 aaaatggtgt tgttgcagga atagaagttg caaaggaaat tttccataca ttcgatccgg
     5161 aactttccat gacaaaattt atcaatgatg gagatgaagt gaaaattggc gatattgctt
     5221 ttgaagttaa aggtaaaatt caatctttgc ttcaaactga acgattgatg ctcaacgtga
     5281 tgcaaagaat gagtggcatt gcaaccaaaa cacgtcaata tgtaaaagca ttagaaggaa
     5341 cgaaaacaaa agtgcttgat acgcgtaaaa ccactccagg tttacgtgcg ttggaaaaag
     5401 aagcggtcag aattggtggt ggcgtaaatc accgctttgg cttatatgat atgattttgc
     5461 tcaaagataa tcacatagat tttgcaggag gaataaagga agctattttt cgaaccaaag
     5521 aatatttgaa aaataaagga aaaaatttga aaattgaaat tgaggtacgt aatttacaag
     5581 aactttcaga agtgttgagt attggtggtg ttgactggat tatgttggac aattttaata
     5641 ttgaaaatac tcaaaaagct gttgaaatgg tagcaggtcg ttatatgata gaatcttcgg
     5701 gtggcattac acttgaaaca cttcgttctt atgctgaatg tggcgttgat tttatttcgg
     5761 ttggagcatt gactcattct gtaaaaagtc tggatatgag cttcaaagca gtaaattttt
     5821 gaatctcaat taaaaatatt ttcccaacga aaaaaatatt tttgtttctt cacataataa
     5881 taaaaaaatt gattattggc ataaatttta atgtatgctt aaacagtatt taaatagtct
     5941 tcaaacaacc tttaatcaag gcgatgcacg tgaagaaagt tattatgaac atttgaagga
     6001 tttattactg aattatgcca atgagcgtca actgaaaaag gttgatatta ccattttacc
     6061 aaaaccaacc gaagctggta atccagattt tcgcatctgg gatggtaaaa atcatattac
     6121 cggctatatc gaagccaaag atccttctgt taccaatctt gacagaatag aagtttcaga
     6181 gcagttgaaa cgttatcgag aaacttttcc aaacattatc ctgagttgtg atttgctttc
     6241 aaattttata tctttgatat ttggaacaac acatctttat atctgtgaaa attagaatcg
     6301 gacactattt cacgaagtgt gtaaattaaa aagttgggat aaaaattaaa gtcttattct
     6361 ttcgccaaag ataaga
//
LOCUS       DAOTKS010000036         6365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_11423_length_6365_cov_4.164818, whole genome shotgun sequence.
ACCESSION   DAOTKS010000036 DAOTKS010000000
VERSION     DAOTKS010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6365
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11423_length_6365_cov_4.164818"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="P5236_01315"
     CDS             complement(<1..893)
                     /locus_tag="P5236_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRU63033.1"
                     /translation="MKVLIATDKPFAKIAVDGIRKEIESAGFELALLEKYTEKQQLLD
                     AVKDVDALIVRSDIIDAEVIAAAKKLKIVVRAGAGYDNIDLDAATKAGICVMNTPGQN
                     SNAVAELVFGLLIYAVRNFFNGTSGTELRGKKLGIHAYGNVGKNVARIAKGFEMKLYA
                     FDPYCQPEDIKKDGVQPLSSIESLYENCEIISIHTPATAETKKSINANLLQKLPKGAV
                     IVNTARKEVINEEDLIACMENRLDIKYVTDIMPDHHAEMVEKFPGRYFSTPKKMGAQT
                     AEANINAGIAAAKQIVDFFKNG"
     gene            complement(1023..2090)
                     /gene="serC"
                     /locus_tag="P5236_01320"
     CDS             complement(1023..2090)
                     /gene="serC"
                     /locus_tag="P5236_01320"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HRU63034.1"
                     /translation="MKKHNFNAGPSILPCEVIEKTAQAVLDFNGSGLSILEISHRSKD
                     FQAVVDEAVALMKELLNIPEGYSVIFLGGGASLQFCMVPYNLLEKKAAYLITGSWASK
                     AYKEAKSFGEAVEVASSKDANFTYIPKNYVIPADADYFHITTNNTIYGTELLKDIDSP
                     VPLVADMSSDIFSRPVDVSKYGIIYGGAQKNLGPAGVAFVIIKESILGKVSRYIPTML
                     NYKTHIENNSLFNTPPVLPIYSSLETLRWLKAQGGVKEMEKRSIAKSELLYNEIDRNK
                     LFVGTAAKEDRSRMNVCFVMKPEYQELEAEFLKFATDRGMVGIKGHRSVGGFRASLYN
                     ALPIESVQALIDCMQEFEKLH"
     assembly_gap    2602..2701
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2702..4361
                     /locus_tag="P5236_01325"
     CDS             <2702..4361
                     /locus_tag="P5236_01325"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HRU63035.1"
                     /translation="MAGYLTILPGIFLIMSLWVSSKVINKIFKVYFAIVALLISIIFV
                     ADISLYGYWGFRLDATVLFYLKWPKEAMASVNVLTVIIGIIVIAIIFILLYYLFTHFL
                     LKEKFSQKGNENKFLNAFVLLLFTGLLFIPIRGGFDVSTMNVSKAYFSSRIELNHAAI
                     NPMFNFMESLFKEQDFDRQYRFMSDKEAHEEFKKLIDQPTDKEIPALFSLQRPNIILI
                     ILESFGSKIIEPLGGIPGIAPHLNHYCDEGILFTNFYANSFRTDRGLVSILSGYPAQP
                     TTSIMKYPRKSQTLPSISRSLKQNDYQVAYYYGGDADFTNMRSYLISQGVDELITKQD
                     FPRKEHTMKWGVHDHIVFQRFLSDLQKEQKEPFMRIIQTLSSHEPFEVPYHKNNDSYL
                     NSVQYTDSCLGEFIEEFKKTKYWNNSIVLLVPDHAMHYPSSIDIRSVERYKIPFLILG
                     GAVISHQKIDTYASQIDIAATLLHQLQISHQEFIFSKNILNPDSPHFAFYTFPNGFGF
                     LTPQNYYVYDCASQSVVINEGKKFENKKKGEAYLQILYDDLASR"
     gene            4703..5455
                     /locus_tag="P5236_01330"
     CDS             4703..5455
                     /locus_tag="P5236_01330"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HRU63036.1"
                     /translation="MKRLENKVAIVTGAARGMGKAEAELFAQEGAKVVLADILEKEVA
                     EVANQINKNGGEAIAFKLDVSKAKDWEKVVNEVVKKWGKVDVLVNNAGILSLSGIEET
                     SEEEWDKVMEINAKSDFLGIKYVLPAMKMAKKGSIVNISSIYGLIGSGAAVAYHASKG
                     AVRLLTKTVAAELAKYNIRVNSIHPGVIKTPMTEELLKDPANTKNLLGTTVLGRPAEA
                     IEVAYGALFLASDESSFITGSELVIDGGYTAL"
     gene            complement(5687..>6365)
                     /locus_tag="P5236_01335"
     CDS             complement(5687..>6365)
                     /locus_tag="P5236_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRU63037.1"
                     /translation="RRNVDINVLIFNNQIYGLTKGQYSPTTKKGFVTKSSPYGTIERP
                     FRLGELTLGARGTFFARTLDVDLVTSQMVMKAALHHKGTSVVECLVNCIIFNKGAHGW
                     LSEKETRADRTIVLEHGKRMIFGRNKDKGLILENNELKVVTIGENNITENDILIHDAT
                     TTDYSLQMKLALMEGPDMPVALGIIRDVKEETFEDAMEKQIKSVQNSSTIKTFDDLIA
                     QCEQWEI"
BASE COUNT         2018 a         1044 c          998 g         2205 t
ORIGIN      
        1 ccatttttaa agaaatctac aatttgtttg gctgctgcaa ttccagcatt aatgtttgct
       61 tctgctgtct gagcgcccat ttttttagga gttgagaaat atcgtcctgg aaatttttct
      121 accatttctg catgatgatc gggcataata tctgttacat attttatatc caatcggttt
      181 tccatgcatg ctattaagtc ttcttcattt attacttctt ttcgagcagt attcacaatc
      241 actgctcctt tcggtaattt ttgaagtaaa ttggcattga tagatttttt tgtttcagca
      301 gtggcaggcg tatgaataga aataatttca caattttcat aaagtgattc aatagaagat
      361 aaaggctgaa caccgtcttt tttaatgtct tcaggctgac aataaggatc aaaagcatat
      421 agtttcattt cgaaaccttt agctatacga gctacgtttt tccctacatt cccataagca
      481 tggatgccca gttttttccc tcttaattcg gtccccgaag tgccattgaa aaaatttctt
      541 actgcataaa ttaacaaacc aaaaaccaat tcggcaactg catttgaatt ttgtccagga
      601 gtattcatta cacagatacc cgcttttgta gcagcatcaa gatcaatatt gtcataacct
      661 gcaccggcac gaacaacgat tttcaatttt tttgcagcag caataacttc agcatcaata
      721 atatcacttc taacaatcag agcatcaaca tctttcactg catccagaag ttgttgcttt
      781 tccgtatatt tttcaagcaa tgccagctca aaaccagccg attcaatttc ttttcgtatg
      841 ccatccactg caatttttgc aaatggttta tcggtagcaa taagtacttt catataaaat
      901 aattttaaag gtttaactta tcatatagaa gcaaaacttt ttttcaatgt tttctcctta
      961 taaaagagaa atcattacca ttttcttatt tataacgaaa tctatcaaag taaattaaaa
     1021 aattaatgca atttttcaaa ttcctgcata caatcaatta aagcctgaac actttcaata
     1081 ggcaaagcat tgtaaagaga agctctgaaa ccacctactg agcgatgacc tttgatgcct
     1141 accatgccgc gatcggtagc aaattttaag aattctgctt caagttcctg atattcaggt
     1201 ttcataacaa aacatacatt cattcgagaa cgatcttcct tagctgctgt accaacaaaa
     1261 agtttgtttc ggtcaatttc attgtaaagt aattctgatt ttgcaatact ccgtttttcc
     1321 atttctttta ctcctccttg tgccttcaac cagcgtaaag tttccagaga agaatagata
     1381 ggaagaacag gtggtgtatt gaacaaagaa ttgttttcaa tatgggtttt ataattaagc
     1441 atcgtaggaa tataacgcga tacttttcct aaaatagatt cttttataat aacaaatgcc
     1501 acacctgctg gtcctaaatt tttttgagct ccaccgtaaa taattccata ttttgaaaca
     1561 tccacgggac gagaaaaaat atcagaagac atatcagcta ctaaaggaac tggcgagtca
     1621 atatctttta acaattcagt tccataaata gtgttattgg tagtaatatg aaaataatct
     1681 gcatcagcag gaatcacata atttttggga atataagtaa agttggcatc ttttgaagat
     1741 gctacttcta ctgcttctcc aaaacttttt gcttctttgt aagctttact tgcccaactg
     1801 ccggtaatta aataggcagc ctttttttcc agcaaattgt atggaaccat gcaaaattgt
     1861 aaacttgctc cacctcctaa gaaaatcaca gaataacctt caggaatatt gagcaattct
     1921 ttcattaaag ctactgcttc atctacaact gcttgaaaat cttttgatcg atgactgatt
     1981 tccagaatag acaaacctga accattaaaa tccaacacag cttgagctgt tttttcaatt
     2041 acttcgcaag gtaaaataga aggccctgca ttaaaattat gctttttcat atcaaaaatt
     2101 aaatgattat ttgtaaaatt tgttactttt caaatgaaaa agcaaagtta catttttttt
     2161 taaatttatt ctttttaatg actaattttt taatatttat ctttaaactt ttcaatttgt
     2221 acgttttttt gagaaaaaat attttatttg agaaagaaaa ttgcacaaaa aaactatctt
     2281 tgtgtaattt tttaatattg ttaatagaca agaaatttgc aattttgtaa aaaattatct
     2341 caactgcaat ttttatagtc agatattaat tttttctcat aaatgatcag caaacttatt
     2401 caatttatta aaatttatct cttttttatc ctgattttcc tcgttcagaa accgcttttc
     2461 atgttgtatt accataattt gtatcacgat atctctttcc ttgattttgt tcgtgtgctc
     2521 ggcaacggat taaaattaga tgcatctatg gctggctatt tgacgatatt gccgggaatt
     2581 tttctaatta tgtcattgtg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 ntatggctgg ctatttgacg atattgccgg gaatttttct aattatgtca ttgtgggtat
     2761 catctaaagt tatcaataaa atttttaagg tttactttgc cattgtagct ttgcttattt
     2821 caataatttt tgtagcagat atttctctgt atggttattg gggatttcga ttagatgcga
     2881 cggtgctttt ttacctgaaa tggccgaaag aagcgatggc gagtgtaaat gtgctaactg
     2941 ttattattgg gataatagta atagccatca tttttatttt attgtattat ttgtttactc
     3001 attttttatt aaaagagaag ttttctcaaa agggaaatga aaataaattt ctgaatgctt
     3061 ttgtgttgct attatttact ggtttacttt ttattcctat tcgtggcggt tttgatgtct
     3121 ccacaatgaa tgtcagtaaa gcttatttta gttcacgaat cgaattgaat catgccgcta
     3181 ttaatcctat gtttaatttt atggaatctc tctttaagga gcaggacttt gaccgccaat
     3241 atcgttttat gagcgataaa gaagctcacg aggagtttaa aaaattgatt gatcaaccta
     3301 ccgataaaga aattcctgct ttattttcct tgcaacgtcc caatattatt ctgattatat
     3361 tggagagttt tgggtcgaaa ataattgagc ctctgggtgg aattccagga attgctccac
     3421 atttgaatca ctattgcgat gaaggaattt tatttactaa tttttatgca aacagtttca
     3481 gaaccgatcg tggacttgtt tcaattttaa gtggttatcc tgctcaaccc accacttcta
     3541 ttatgaaata tccacgaaaa agccaaacgt taccttctat ttccagatca ttaaaacaga
     3601 atgattatca ggtagcatat tattatggcg gagatgctga tttcacaaac atgcgttctt
     3661 atttaatctc acagggagtc gatgaactta ttacgaaaca agactttccc agaaaagaac
     3721 atacaatgaa atggggcgtc catgatcata tcgtgtttca acgttttctg tccgatttac
     3781 aaaaggaaca aaaagaacct tttatgcgaa ttatacagac attaagcagc catgaacctt
     3841 ttgaagttcc ttaccataaa aacaatgatt cttacctgaa ttccgttcaa tatacggata
     3901 gctgcttggg tgagttcatt gaggaattta aaaaaacaaa atattggaat aattcaattg
     3961 tactactggt tcccgatcat gccatgcatt atccatcctc tatcgatatc cgttctgttg
     4021 aacgttacaa aatacctttt cttattttag gaggtgctgt aatatcccat caaaaaattg
     4081 atacatatgc ttcacaaata gatattgctg ctaccctcct gcatcagttg caaatttctc
     4141 atcaggaatt tatttttagt aaaaatattt tgaatcctga ttctcctcat tttgcttttt
     4201 atacttttcc taatggtttt ggttttttaa ctcctcaaaa ttattatgtg tacgattgtg
     4261 cttcccaatc agtagtaata aatgaaggaa agaaatttga aaacaaaaaa aagggtgaag
     4321 cctatcttca gattctttat gatgatctgg caagtagata aaaagtatat ttttcaaaaa
     4381 aattttagac aaagattaga aatacttctg aattattacg aaaaaacaac ctaaaaaaac
     4441 ctctttctaa acttttttgt tccccttcat aaataccttt ttcaattgga aattcaacaa
     4501 aataaataaa taaattttgt ttttacttta tagggaagca taaaaaaaat ttttaaagct
     4561 gttaaaaata acatcacgca agttttttaa gcaaaacttt gtaaattcaa aattaaaatt
     4621 ttaaatttga cttttattaa aaataaagtt ttttataaat gatcttgtca aatttaatgt
     4681 ataactaata aaataaaaaa ttatgaaaag attagaaaac aaagttgcaa ttgtgacagg
     4741 tgcagcaaga ggtatgggta aagctgaagc tgaattattt gcccaagaag gtgctaaagt
     4801 ggtattggct gatattttag aaaaggaggt agcagaagtg gcaaaccaga ttaacaaaaa
     4861 cggtggtgaa gctattgctt ttaaattgga tgtttcaaaa gctaaagatt gggaaaaagt
     4921 ggtgaatgaa gttgtaaaaa aatggggcaa agtagatgtg ctggttaata atgctggtat
     4981 tttgagtctg tcaggcatag aagaaactag cgaagaggaa tgggataaag ttatggaaat
     5041 taatgccaaa agtgatttct taggcattaa atatgtattg ccagcgatga aaatggcaaa
     5101 gaaaggctct atagttaata tctcttctat ttatggttta ataggcagtg gtgcggctgt
     5161 ggcataccat gcttctaaag gggctgttcg tttgttgaca aaaaccgtag ctgctgaatt
     5221 agccaaatat aacattcgtg ttaactctat tcatccaggc gtaattaaaa ctcctatgac
     5281 tgaggaatta ttaaaagatc cagccaatac aaagaatttg ctgggaacaa ctgttttagg
     5341 aagacctgct gaagctattg aggtagctta tggagcatta tttttggctt ccgatgaatc
     5401 ttccttcata actggttctg aattagtaat agacggtgga tacaccgctc tttaactata
     5461 tactatacga gaggtgttaa taaaattgac gcctctcgtg tagaaaacaa taatacttaa
     5521 aaatatattt atcactttgt cactggtata aaaagacaaa aaagtaaagt atttattcct
     5581 tcaagataaa aatatttctt tgcctcatta ttttccctta aaagttgcgt taattatttg
     5641 atttttcgaa aattatcatg aaaatttaaa agcaagcgag gattgattat atttcccatt
     5701 gttcacattg agcaatcaag tcgtcaaatg ttttaatagt agaagagttt tgtacacttt
     5761 taatttgttt ttccattgca tcttcaaaag tttcttcttt cacatcacgg ataatgccta
     5821 aagctactgg catgtcagga ccttccatta atgcaagttt catttgcaag ctataatcag
     5881 tagttgttgc atcatgaatt aaaatatcat tttcagtgat attattttct ccgatggtta
     5941 ctactttcaa ctcgttgttt tctaaaatca gacctttatc tttatttctt ccgaaaatca
     6001 tgcgttttcc atgttcaaga acaattgttc tatcggctct ggtttctttt tctgacaacc
     6061 agccatgcgc tcccttgtta aatatgatac agttgaccag acattctaca actgacgtcc
     6121 ccttgtgatg taaagctgct ttcatcacca tttgagaagt aactaaatct acgtccaatg
     6181 tgcgagcaaa gaaagtgcct cttgctccaa gtgtgagttc tcctagtcta aatggacgtt
     6241 caatggttcc ataaggagat gatttggtaa caaatccttt ttttgttgta ggagaatatt
     6301 gaccttttgt taatccatag atttgattat taaaaataag tacgttgata tccacatttc
     6361 tcctt
//
LOCUS       DAOTKS010000037         6325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_11498_length_6325_cov_4.558373, whole genome shotgun sequence.
ACCESSION   DAOTKS010000037 DAOTKS010000000
VERSION     DAOTKS010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6325
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11498_length_6325_cov_4.558373"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1429
                     /gene="metK"
                     /locus_tag="P5236_01340"
     CDS             149..1429
                     /gene="metK"
                     /locus_tag="P5236_01340"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRU63038.1"
                     /translation="MAYLFSSESVSEGHPDKVADRISDALVDNFLALDKNSKVACETL
                     VTTGQVIIAGEVKTSAYVDVQSVARQTINKIGYTKSEYMFDGNSCGVLSALHEQSPDI
                     ARGVEREDPMEQGAGDQGMMFGYACNETNNYMPIALDIAHTLVSELAKIRKEGKEMTY
                     LRPDAKSQVTIEYDDHNRPLRVHTIIVSTQHDEFIKAGNGKTQEEADIEMLSVIQKDI
                     KNILIPRVIIKDQQFERLFKDDYRLLVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKV
                     AHGGGAFSGKDPSKVDRSAAYAVRHIAKNMVAAGIADEVLIQIAYAIGVSKPINFYVN
                     TFGTAKIKRSDEEISKKIEKLFDLRPKAIEESLKLRNSIYEETAAYGHFGRTPIEVKK
                     YFQDGNGNKISHIVELFTWEKLDKIEEIRKAFQL"
     gene            complement(1656..2102)
                     /locus_tag="P5236_01345"
     CDS             complement(1656..2102)
                     /locus_tag="P5236_01345"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRU63039.1"
                     /translation="MTIAFSGCKSKQKVTEIPGANVKATTTSASTTPSETTAPVVVTT
                     PPSETETNIPEVTRKESFNLAEGETNHDALSYKYHVVVGSFKNLSNAKGLQSTLKQEG
                     NNSLIVINEQGMYRVIIDSFNDYYQAHARINQIKNRFPDAWVLIQK"
     gene            2519..4141
                     /locus_tag="P5236_01350"
     CDS             2519..4141
                     /locus_tag="P5236_01350"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRU63040.1"
                     /translation="MKDTKYIFVTGGVVSSLGKGIIAASIGKLLQSRGFKVTNQKIDP
                     YINIDPGTLNPYEHGECYVTVDGHEADLDLGHYERFLNIETHKENNFTTGRIYQTVIE
                     KERRGDYLGKTVQVIPHITDEIKRNIKLLGSRNGYDFVITEIGGTVGDIESLPYIESV
                     RQLRWELGKNCLVVHLTYVPYITAAQELKTKPTQHSVKTLQEQGVQPDIIVLRTEHNI
                     PADLRKKVALFCNVEPSAVMQSIDVSTIYRVPLNMHEEKLDEILLSKMGFDLTKTPSA
                     DLDKWRHFVEKLENAKDEIHIALIGKYVQLPDAYKSITESLLQACAYNDKKLQLDLIL
                     SDKLNEDNVAKKLANADGVIIAPGFGQRGIEGKFAAIKYTREHNIPTLGICLGMQCMA
                     IEFARNVLGYADANSTEFDPTTSHKVVDIMEEQKNILNLGGSMRVGAYPCSLKKGTKV
                     YEIYQTDNIYERHRHRFEFNNDYKEEFEKAGMICSGINPASNLIETIELQSHNWYIGV
                     QFHPEYHSTVVNPHPLFISFIKAAIEKQNKKI"
     gene            4187..6091
                     /gene="yidC"
                     /locus_tag="P5236_01355"
     CDS             4187..6091
                     /gene="yidC"
                     /locus_tag="P5236_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRU63041.1"
                     /translation="MDKNTFIGFLLIAVILIVFSWLNRPSPEMVEKQKRYNDSIARVQ
                     QIKEESEAVKYQADSLLKSPLLKKDSLTNSILEDAYGEFSVAAVGEEKFITIENEFLK
                     ITFTNKGAQIYSVELKDYRRHDSLPLILFEGKENKMNFTFITNNNRIVNTENLYFEPL
                     PEIKKDKNGNQTIIFRLKTTGEGYLDFAYTIPPKEYMIGFGIKAYHMNNVVPTSINSL
                     EMQWISKIRQQEKGRQFESRYSMIQYKYLSGDTEKLNPNKDDSKEIANKIKWIAFKDQ
                     FFSSVLIAEDAFTSTTLKSTIEKTDSPYLKSCSAEMTVPFDISGKEPTVFRMYFGPNK
                     FNILASYDKGVDKNEKLKLQSLVPLGWGIFGWINRFLIIPMFNFFSKFISNYGIIILL
                     MTIVIKIIIFPLTYKSYTSSAKMRVLKPEIDEIYKRIPPEKAAERQKAVMDLYSRVGI
                     SPMSGCIPALLQIPILFAMFSFFPASIELRQQRFLWAEDLSTYDSILNWNAYIPIITP
                     YFGNHISLFCLLMTISSIVATRLNMANSTPSDQPGGNLMKWMMYLMPVMFMFIFNNYS
                     AALSYYYFISTLITIIQTYVIRKMVDEKKLLAELHAKRNTKKPVKKSSFIERLEKMQR
                     EQEKALKNRK"
BASE COUNT         2202 a          938 c         1119 g         2066 t
ORIGIN      
        1 gtggaaagat ttgagctgct tgcaaccaca gaaaaaaatg ctaaaattgc gtaccatttt
       61 ctgcttgcca tttataagta ttttcgaatc ttcctgcctg taatttaaaa ttcaactacc
      121 attttaacaa ttttaaatta ttaaaatcat ggcgtattta tttagttctg aatcggtttc
      181 tgaaggacat cccgacaaag tagcggatcg aatttctgat gcattagtgg ataatttttt
      241 agcattggat aaaaattcaa aagtagcgtg tgaaactctt gtaacgacag gacaagtaat
      301 cattgcagga gaagtcaaaa caagtgctta tgtagatgtt caatctgttg cgcgacaaac
      361 cattaataaa ataggttata caaaaagtga atatatgttt gatggaaaca gttgtggcgt
      421 attgtcggct ttgcatgaac aatcacccga tattgctcgc ggtgttgagc gcgaagatcc
      481 gatggaacaa ggagcaggtg accagggaat gatgtttggt tatgcttgca atgaaaccaa
      541 caattatatg cctatcgctc tcgatattgc tcatacatta gtaagcgaac tggcaaaaat
      601 tcgtaaagaa gggaaagaaa tgacttatct tcgccctgat gctaaatctc aggtaactat
      661 tgaatatgat gatcacaatc gtcctttaag agttcatact attattgttt ccacacaaca
      721 tgatgaattt ataaaagctg gtaatggaaa aactcaagaa gaagctgata tagaaatgct
      781 ttctgttatt caaaaagata ttaaaaatat tttgattccg agagtaataa taaaagatca
      841 acaatttgaa cggcttttta aagatgatta tcgtttgtta gttaatccta ctggaaaatt
      901 tgttattgga ggtccacatg gagatacagg tttaacagga agaaaaatta ttgtggatac
      961 ttatggagga aaagtagctc atggaggagg agctttttca ggaaaagacc catcaaaagt
     1021 agatcgttcg gctgcctatg ctgtccgtca tatcgccaaa aatatggttg ccgcaggcat
     1081 agccgatgaa gtattgatac agattgcata tgcaattgga gtgtctaaac ccattaattt
     1141 ttacgtaaat acctttggta cagctaaaat aaaaaggagc gatgaagaaa tttcgaaaaa
     1201 aattgaaaag ctctttgatt tgcgtccaaa agccatagaa gaaagtttaa aactacgtaa
     1261 ttcaatttat gaagaaaccg cagcctatgg acattttgga agaaccccca ttgaagtaaa
     1321 aaaatatttt caagacggaa atggcaataa aatttcacat attgtagaac ttttcacttg
     1381 ggaaaaattg gacaaaatag aagaaatccg aaaagctttt caactttgat ttctttttta
     1441 atcgtaagaa ttccccgatg tttgcgtcgg ggtagctttt gtttttattt ttttagaaat
     1501 tccggtggcg aagccataaa attggcgtaa tacctcgctg cttgcggcga ggatagtcaa
     1561 ttttaaggaa tttctttatt caaatgaaaa caaaaaaacc gttactattt tctattagtg
     1621 aacggttttt taaatatcga ttttgaaaaa taattttatt tttgtatcaa aacccacgca
     1681 tcagggaatc tattttttat ttgattgata cgtgcatgag cttgataata atcattaaag
     1741 gaatcaataa ttaccctgta cattccttgt tcattgatta cgattagaga attattacct
     1801 tcttgtttta atgtgctttg taagcctttc gcatttgaca agtttttaaa acttccaact
     1861 actacatgat atttgtaact taaagcatca tgatttgttt caccttcagc caaattaaag
     1921 ctttctttac gtgtaacttc tggtatatta gtttcggtct ctgaaggcgg agtagttaca
     1981 acaacaggag cagtagtttc tgaaggagta gtagaagcag aggtggtggt agcttttaca
     2041 tttgctccag gaatttctgt caccttttgt tttgatttgc aaccagagaa agctattgtc
     2101 atgagcgaca atattattaa acagttcaaa aaatttctga ttttcatatt gatttttttt
     2161 atgattataa aacgtgcttt tccattgcaa agatggaaac aaaattagaa atatacaaaa
     2221 ttttatgatt ttattttatc caattttttg agttcgatat tcataaattt atttttaaaa
     2281 gattgcttcg ctaattttta aaattcagta ttgatattca ataacttaca aattataaaa
     2341 tccttaaaat taatttgtga gaattatacg ttattttcaa aaaaacatag ttttttttta
     2401 atagtcagaa gaatcaagga tttttttaat aaaaaaggaa aatactttgt atcctttcaa
     2461 tattcatttt tttttccgaa atttgcagct ttaattaagt taagttatta taggtaaagt
     2521 gaaagatacc aagtatattt tcgtaacggg tggcgttgtt tcttcattag gtaaaggcat
     2581 tattgctgcc tcaataggta aattattaca atcaagaggt tttaaagtaa ctaatcaaaa
     2641 aattgatcct tatatcaata tcgatccagg aactttaaat ccttatgaac atggtgaatg
     2701 ttacgtcaca gtagatggac acgaagccga tctggattta ggtcattacg agcgattttt
     2761 aaatatagaa acgcataagg aaaataattt tactacagga cgaatttatc agacagttat
     2821 tgaaaaagaa cggagaggag attatttggg aaaaactgta caggtaatac ctcacatcac
     2881 tgacgaaatt aaacgcaata ttaaattatt aggcagcaga aatgggtacg attttgtcat
     2941 taccgaaata ggtgggacag taggcgatat tgaatccttg ccctacattg aaagtgtacg
     3001 tcaattacgt tgggaactgg gtaaaaattg ccttgtcgtt catttaactt atgtgcctta
     3061 tattactgct gcacaggaat taaaaactaa acccactcaa cattctgtaa aaactttaca
     3121 ggaacaggga gtacaacctg atattattgt tttacgaaca gaacataata ttcctgctga
     3181 tttacgaaaa aaagtagcct tattttgcaa tgtggagcca tcggcggtta tgcaatctat
     3241 tgatgtttca actatttaca gagtaccatt gaatatgcac gaagaaaagc tggatgaaat
     3301 tcttttgagt aaaatgggat tcgatttaac taaaacacct tctgctgatc tggataaatg
     3361 gagacatttt gtagaaaaac ttgaaaatgc aaaagatgaa attcatatcg ctctgatagg
     3421 aaaatatgtt caacttccag atgcttacaa atctattacc gaatctcttc tccaggcatg
     3481 tgcttataac gataaaaaat tacaactgga tctaatttta tcagataaat taaatgaaga
     3541 taatgtagca aaaaaattag ctaatgcaga tggagtaatt atagcgccag gttttgggca
     3601 acgaggaata gaaggaaaat ttgcagctat aaaatatact cgtgaacaca atattcctac
     3661 attgggaatt tgtttgggaa tgcaatgtat ggctattgaa tttgctcgaa atgtattggg
     3721 ttacgctgat gcaaatagta cagagtttga ccccactaca tctcataagg tagtggacat
     3781 tatggaggag caaaaaaaca tattaaatct tggtggaagt atgcgagtgg gagcttatcc
     3841 atgctcttta aaaaaaggaa cgaaagttta tgaaatttat caaacagata atatttatga
     3901 gcgtcatcga catcgttttg aatttaataa tgactataaa gaagagttcg aaaaagctgg
     3961 aatgatttgc agtggtatca atccagcttc taacctcata gaaacaattg aattacaatc
     4021 gcataactgg tatattggag ttcaatttca tccagaatat catagtacag tagtgaatcc
     4081 tcatcccctc tttattagtt ttattaaagc ggctatagaa aaacaaaata aaaaaattta
     4141 aatttttata acactaagga aaaggatagg ttgaaattta taaaacatgg ataaaaatac
     4201 gtttataggt tttttgttga ttgcagttat tctcattgtg ttttcgtggt taaacaggcc
     4261 atcgcctgaa atggttgaaa aacaaaaacg atataatgat tctattgcca gagttcagca
     4321 gataaaagaa gaaagtgaag cagtaaaata tcaggctgat tctttgctga aatcaccttt
     4381 actcaaaaaa gattcactca ccaattctat tctcgaagat gcttatggag agtttagtgt
     4441 agctgctgta ggagaagaaa aatttatcac tattgaaaat gaatttctaa agattacttt
     4501 taccaataaa ggagctcaaa tttattcggt ggaactgaaa gattaccgcc gacacgattc
     4561 tttgccttta atattatttg aaggaaaaga aaacaaaatg aattttactt ttattaccaa
     4621 taacaatcgt attgtcaata cagaaaattt gtatttcgaa cctcttcctg aaatcaaaaa
     4681 ggacaaaaat ggaaatcaaa ctattatttt tcgtctgaaa accaccggag aaggttatct
     4741 ggattttgcc tatactattc ccccaaagga gtatatgata ggctttggta tcaaagctta
     4801 tcatatgaat aatgttgtac caacaagcat taattcgctt gaaatgcaat ggatttcaaa
     4861 aattcgtcaa caggaaaaag gacggcaatt tgaatcacgt tactcaatga ttcaatataa
     4921 atatttatcg ggcgacacgg aaaaacttaa tcctaataaa gacgacagca aagaaatagc
     4981 taataaaata aaatggattg cttttaaaga tcagtttttc agcagtgttt tgattgcgga
     5041 agatgctttt acctcaacca cattaaaaag tacgatagaa aaaacggact caccttattt
     5101 aaaatcatgt agtgctgaaa tgacggtgcc ttttgatatt tctggtaaag aacccactgt
     5161 atttcggatg tactttggtc ctaataaatt taatattctt gcaagttatg acaagggcgt
     5221 agataaaaac gaaaaattaa agcttcaaag tttagtgccg cttggttggg gaatttttgg
     5281 atggattaat cgttttctta ttattccaat gttcaacttt tttagtaaat ttattagtaa
     5341 ttatggaatt attattcttt taatgactat tgttattaag attataattt tcccacttac
     5401 ctataaatcc tatacttcga gtgcaaaaat gcgtgttttg aaaccagaaa tagatgaaat
     5461 ttacaaacgt attcctccag aaaaagcagc agaacgtcaa aaagcagtga tggatctata
     5521 ttcaagagta ggtatcagtc cgatgagtgg atgcattcca gctttgctgc aaattcctat
     5581 tttgtttgcg atgtttagct tttttcctgc ttccatcgag ctcagacagc aaagatttct
     5641 ttgggcagaa gatctttcca cttacgattc tattttaaac tggaatgctt atattcctat
     5701 tattactcct tattttggaa accatattag cttgttttgt ttgttgatga ccatcagcag
     5761 tattgtagca acccggttaa atatggctaa ttctacacct tccgatcagc ctggtggaaa
     5821 tctgatgaag tggatgatgt atttgatgcc cgtgatgttt atgtttatat tcaacaatta
     5881 ttcggcagca ttgagttatt actattttat ttccacttta atcactatca ttcaaaccta
     5941 tgttatccgc aaaatggttg atgaaaaaaa attattggca gaattgcatg ctaaacgcaa
     6001 tactaaaaag cctgtcaaaa aaagtagttt tattgaacgt ttagaaaaaa tgcaacgcga
     6061 gcaagaaaaa gctttgaaaa acagaaaata agttttgtat ctcggctaac ttaaacagaa
     6121 agtagaaaaa tattattttc gttgttcatt ttttaaaatt tattttccct atgaaattca
     6181 aaggaattat tttcacaagt attgttatta ctttgctttt ttgttcgtgt aaaaaaacaa
     6241 atcgttttgc cataaacact tcaaaagata ggataaaagt ggttattcaa cggtttgata
     6301 aagatttgat tttacttgat acaat
//
LOCUS       DAOTKS010000038         6292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_11560_length_6292_cov_4.678371, whole genome shotgun sequence.
ACCESSION   DAOTKS010000038 DAOTKS010000000
VERSION     DAOTKS010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6292
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11560_length_6292_cov_4.678371"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..901
                     /locus_tag="P5236_01360"
     CDS             125..901
                     /locus_tag="P5236_01360"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HRU63042.1"
                     /translation="MQKFWYLTVFMLWIIFQFTSCITARKVNYLQKPSQTIPAYKDSV
                     TFEDYRLKGGDRLDIKVYSMDEKTNTLFNGVNNFESGSYGGSLNSDLYTYLIKPDGTI
                     SLPMIGKVFVEGQTQREAKKTIENAIQSVIKINTVDVKLVEKYYSIISSSTSGRFPIT
                     REKINIFEALAMAGDIGTYGDRSKIRIIRETENGTQIKMFDVRSADIIHSEFYYVEPN
                     DVIYIQDVNEQFFSVTSFPSLLATLISTFSLAIFIYDLVK"
     gene            898..1686
                     /locus_tag="P5236_01365"
     CDS             898..1686
                     /locus_tag="P5236_01365"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HRU63043.1"
                     /translation="MINKILHFFYNIKITENFVRLFLTVLPFLCLTSCYNYKSVGLLQ
                     ENNHSLPQYEKQLYEDYKLCVNDEIVFRLITTDETISKLISSNSPRAGQQISYRVYPD
                     GTIDIPFLSQIPVKGLTLNEATEVIQNRIREIIPDAEIKLSLANKSFTVIGDIGSGTF
                     PIYKEKMTIFQALALSGDLQLSGDRKHVRILRNIEGKTNILEFDIRPASIVESKYYYI
                     YPNDIIYVQRAPSSFYKVNNYNSFLGLITTSLTFLISVIYLLKL"
     gene            1712..4060
                     /locus_tag="P5236_01370"
     CDS             1712..4060
                     /locus_tag="P5236_01370"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HRU63044.1"
                     /translation="MDLEQKNHTQAFVDEESSFDIREWLHYFLQHWYLFLCFVILFIM
                     LIYAKNRRWMPLYETAGTIIMAENKYLPQNQFFLGGFNVGGYQNVNDQLVILNSYDLI
                     GKVVDSLEFMKVDYLTIGRFKTRNIYRNTPIYIQSNYISPDAYQTVFKIKLFSDNSFI
                     ITPYKNKNFGNFNIEGHFGEPIHHNLFFITVNKLTNIPYSGEIEMYFRFRTRESLINE
                     FSSRLSSKYVMDGSSVLRVSLMGETPERDIDFLNKLFEVFLNDNLAKKNDVANKTIYF
                     INEQLDILSQSLAFSENKLTSFKQSNQIIDISSHASEIINKLNFYEEKNNQIKLKETY
                     LNYLSDYLKTNFEEGKVIAPSNLGLNEPILMQLVQQINDLYLQRSELSPKNIYYEKYT
                     NEIENVKTAIFELIKSIRTSLKIEKDENNRQIALLNNEMKKLPEKELAMNAIERDYKL
                     NDNYYTFLLQKKAEAEIQKASNSPDNSVLENARIISITNNKEKSKTTTRYLILAFLIP
                     SLIIVLKKSLNNTITSITDIEKKSSFPVIGVVLHTKNDDPLLCAKYPNSLFSEMFRII
                     RTRIEFIAQRKKNIVVLVTSLTSEDGKTFFSTNLAAIYGMTGEKTLLIDMDIRKPSVN
                     KRLNVSHPKGVTNYLIGECTLPEIINKIDNVNFDIIFAGTVPPNPGELIRSEKLIKMF
                     EELKKQYDYIIIDVSPVGLVSDTYSLVPLSDVNLLVVRNKKTNKTFFKNIATQLMEDH
                     IQNFYVILNDVDTSYGGYEKSYKYHMYDKKTDNKYYDMIDEI"
     gene            4090..5241
                     /gene="gmd"
                     /locus_tag="P5236_01375"
     CDS             4090..5241
                     /gene="gmd"
                     /locus_tag="P5236_01375"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRU63045.1"
                     /translation="MNKKVALITGITGQDGAYLAELLLSKGYIVHGIKRRSSLFNTER
                     IDHLYQDPHEKNRNFILHYGDMTDSLNLTRIIQETQPDEIYNLGAMSHVKVSFDTPEY
                     TANADGIGTLRMLEAIRLLNLTEKTRFYQASTSELYGLVQEIPQRETTPFYPRSPYAV
                     AKLYAYWITVNYREAYGLHASNGILFNHESPLRGETFVTRKITRAAARIALGLQDKLF
                     LGNLSSRRDWGHAKDYVYAMYLILLQDQPDDYVIATGKSTEVREFARLSFEQIGIKII
                     FVGNGVEEKGYIDKIDEDIFVEQVGEKFLTNVKNIKESVIEVDPNYFRPTEVDYLIGD
                     ATKAKTKLNWTPQYDLPALISEMMKSDVQLMKKEMHLKQGGFRILNHYE"
     gene            5331..>6292
                     /locus_tag="P5236_01380"
     CDS             5331..>6292
                     /locus_tag="P5236_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008999318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HRU63046.1"
                     /translation="MEKNSKIYVAGHTGLVGSAIKRNLEKNGFTNIVGRTQAELNLLK
                     EAPTEAFFKEEKPEYVFLAAAKVGGIVANNTFRAQFIYENLMIQNHVIHQSYLYGVKK
                     LLFLGSSCIYPRLAPQPIKEEYLLTGPLEYTNEPYAIAKIAGIKMCEAYHTQYGCNFI
                     SIMPTNLYGPNDNYDLEKSHVLPALIRKMVLGKYLMENDWENLRTDLTRLPIEGVNGS
                     YSKEIILKVLEKYGIIHQNGKVVITLWGTGSPLREFLHVDDMADATVFLMQHYDAPDS
                     IPSHVNVGCGEDISIKELSEIVKKIVDYEGEIQWDTTKPDGTPR"
BASE COUNT         2271 a          874 c         1038 g         2109 t
ORIGIN      
        1 tgtactcgga agcacggtac ggtaagcttt taaagttttg tgaccttgat ttttattttc
       61 acgcatttgg aaatacctcg attttcatat atttttgttg tatttttaaa aagatagtag
      121 aaatatgcaa aagttttggt atctcacggt ttttatgtta tggataattt ttcaatttac
      181 ttcttgtatc acagcaagaa aggtcaatta tctacagaaa ccatctcaaa ctattcctgc
      241 ctataaagat agtgttactt ttgaagatta tcgtctaaaa ggaggagaca ggcttgacat
      301 caaagtatat tcgatggatg aaaagactaa tactttattc aatggtgtta ataatttcga
      361 atcaggctct tatggaggtt ctctcaatag tgatttatat acctatttga ttaaacccga
      421 tggcactatt tcattgccaa tgatagggaa agtttttgtt gagggacaaa ctcagcggga
      481 ggcaaaaaaa acaattgaaa atgctattca atctgtgatt aaaataaata cggttgatgt
      541 aaaattggtt gagaaatatt atagtatcat tagttcaagt acttcaggaa gattccctat
      601 tacccgtgaa aaaattaata tttttgaagc attggctatg gcgggcgata ttggaactta
      661 tggtgatcga agtaaaataa gaattattag agaaactgaa aatggtactc aaatcaaaat
      721 gtttgatgta agaagcgcgg atattattca ttcggaattt tattatgtgg agccaaatga
      781 tgttatttac attcaagatg taaatgagca attttttagt gttacttctt tcccttcttt
      841 gttggctact ctcatatcaa ctttttctct ggcaatcttt atatatgatt tagtaaaatg
      901 attaacaaga tattgcattt tttttataat ataaaaatta cggaaaattt cgttcgttta
      961 tttttaaccg ttttgccttt tttatgtttg acttcttgtt ataattataa gagtgttggc
     1021 ttattgcaag aaaacaatca ttcattacct cagtatgaaa aacaacttta tgaagattat
     1081 aaactttgtg taaatgacga aatcgtattt cgtttaataa ccacagacga aacaatttca
     1141 aaactaattt cttctaatag tcccagagca ggtcaacaaa tttcttatcg agtctatcct
     1201 gatgggacaa tagatattcc ttttcttagt caaatacctg ttaaaggatt aactttgaat
     1261 gaagctactg aggtaattca aaatcgaata agggaaataa ttccagatgc agaaataaaa
     1321 ttaagtcttg ctaataaatc atttacagta attggagata ttggaagtgg aacattccct
     1381 atttataaag aaaaaatgac tatttttcaa gctttagctt tatcaggtga tttacagttg
     1441 tcaggtgata gaaaacatgt acgtatttta cgtaatattg agggaaaaac aaacatactt
     1501 gaatttgaca ttcgcccggc aagtattgta gaatcaaaat actattatat ttatccgaac
     1561 gatatcattt atgttcagcg tgccccttca agtttttata aagtaaacaa ttataattct
     1621 tttttagggc taattactac ttctttaact tttttgatta gtgttattta tttattaaaa
     1681 ttgtaattga attaaatttt ttatttagaa aatggattta gagcaaaaaa atcataccca
     1741 agcctttgtt gatgaagaaa gcagctttga tataagagag tggcttcatt attttctaca
     1801 acattggtat ttgttcttat gttttgtgat actttttata atgttgattt atgctaaaaa
     1861 caggaggtgg atgccattat acgagacagc cggaaccatt attatggcag aaaataaata
     1921 tcttcctcaa aaccaatttt ttttaggagg atttaatgtt ggaggttatc aaaatgtaaa
     1981 tgaccaactt gtcatactta attcttatga tttgataggg aaagtagttg atagccttga
     2041 atttatgaaa gttgattatt tgacaatagg gcgttttaaa actcgtaaca tatatcgtaa
     2101 tactcctatt tatatccaat ccaactatat ttcccctgat gcctatcaaa cggtttttaa
     2161 aatcaaatta ttttcagata atagttttat tataactcct tacaagaata aaaattttgg
     2221 aaattttaat attgaaggcc atttcggaga acctatccat cacaatttat tttttatcac
     2281 tgttaataaa ttaacaaata tcccttattc gggggaaata gaaatgtatt ttagattcag
     2341 gacacgcgaa tctttaatta atgaatttag ttcacgttta agtagtaaat acgtaatgga
     2401 tgggtcatca gtgcttcggg tatcgttaat gggggaaact ccagaaagag atatagattt
     2461 tcttaataaa ttatttgagg tatttttaaa tgataattta gcgaaaaaaa acgatgtagc
     2521 taataaaacc atttatttta tcaatgaaca attagatatt ttatctcaat cattggcttt
     2581 ttccgaaaat aaacttacca gttttaaaca gtccaatcaa ataattgata tttcaagcca
     2641 tgcctccgaa ataataaata aattgaattt ttatgaagaa aaaaataatc aaattaaatt
     2701 aaaggaaact tatttgaatt atttatcgga ttatttaaaa actaattttg aagaaggcaa
     2761 agtaatagct ccctcaaatc tgggcttaaa tgaacccata ttaatgcaat tggtacaaca
     2821 aatcaatgat ttatatttac aacgaagcga attaagcccg aaaaatattt attatgaaaa
     2881 atatactaac gaaattgaaa atgtaaaaac agctattttt gaattaatta agagcatacg
     2941 aacttctttg aaaattgaaa aagatgaaaa taatcgtcaa atagctctat taaataatga
     3001 aatgaaaaaa cttcctgaaa aagagttggc aatgaacgca atagaacgcg attataaact
     3061 caatgataat tattacactt ttttacttca aaaaaaagcg gaagcagaaa ttcaaaaagc
     3121 ttccaatagc cctgataata gtgttttgga aaatgcaaga attattagca taactaataa
     3181 caaagaaaaa tcaaagacca caacaaggta tttaatattg gcttttttga ttccttctct
     3241 gataattgtt ttaaagaaat cattaaataa cacgattact tctattacag atatcgaaaa
     3301 aaaatcttct ttcccagtaa taggagtggt tcttcatact aagaatgatg atccgctttt
     3361 atgtgctaaa tatccaaatt cattattcag tgaaatgttt agaatcattc gcacaagaat
     3421 tgaatttata gcccagcgta aaaaaaacat agtcgtatta gttacttctc ttacttcaga
     3481 ggatggaaaa acttttttca gtaccaatct tgctgccatt tatggaatga ctggagagaa
     3541 aacattatta attgatatgg atatcagaaa accaagtgta aataagcgtc taaatgtttc
     3601 tcatcctaaa ggagttacaa attatttaat tggagaatgc actttgccag aaataattaa
     3661 taaaattgat aatgtaaatt ttgatataat ttttgctgga acagtacctc ctaatcctgg
     3721 tgaactgatt cgttctgaaa aattaattaa aatgtttgaa gaactaaaaa agcaatatga
     3781 ttatataatc attgatgtaa gcccagtagg actggtttca gatacttatt ctttggttcc
     3841 tttatcagat gttaatttat tggttgtaag aaataaaaaa acaaataaaa ctttctttaa
     3901 aaatattgcg acacaactta tggaagacca tattcaaaac ttttatgtca ttttaaacga
     3961 tgtagataca agttatggtg gatatgaaaa atcttataaa taccatatgt acgataaaaa
     4021 aaccgataat aagtattatg atatgataga cgaaatttag ttataatcca gataatcaaa
     4081 aattaaatta tgaataaaaa agtagcttta attacaggta taaccgggca agatggtgca
     4141 tatttggcag aattattatt aagtaaaggt tatattgttc atggaataaa acgtcgttct
     4201 tcacttttta atactgaacg aattgaccat ttgtatcaag atcctcacga aaaaaatcgc
     4261 aattttattc tacattatgg agatatgact gactccctaa atttgacccg tattattcag
     4321 gaaacccaac cagatgaaat atacaattta ggagctatga gtcatgtaaa agtaagtttt
     4381 gataccccag aatatacagc taatgctgat ggaataggaa cattgcgaat gctggaagcc
     4441 attcgtttat taaacttgac agagaaaacg cgattttatc aagcttccac ctcagagtta
     4501 tatggattgg tgcaagaaat tccacaacgg gaaacgactc ctttttatcc acgttctcct
     4561 tacgctgtag caaaattgta tgcctactgg attacagtca attataggga agcatacggt
     4621 ttacatgcca gcaatggtat tttgtttaat catgaatcac ctttgcgagg cgaaactttt
     4681 gtaacgcgaa aaataacacg tgcggctgct cgaattgcac tgggacttca ggacaaattg
     4741 tttcttggta atttgtcttc ccgtcgtgac tggggacatg caaaagatta tgtttatgcc
     4801 atgtatttga ttcttctgca ggatcaacct gatgactatg tcattgccac tggaaaatca
     4861 actgaagtac gtgagtttgc tcggttgtca tttgaacaga ttggaataaa aatcattttt
     4921 gttggtaatg gtgtcgaaga gaaaggatat attgataaaa ttgatgaaga tatttttgta
     4981 gaacaagttg gagaaaaatt tttgacaaac gtaaaaaata taaaagaatc tgtgattgaa
     5041 gtagatccaa attattttcg tcctactgaa gtagattatt tgattggtga tgctaccaaa
     5101 gccaaaacaa aacttaattg gactcctcaa tatgatcttc cagcattgat cagcgaaatg
     5161 atgaaatctg atgttcaatt gatgaaaaaa gaaatgcatt taaaacaagg aggattcaga
     5221 attttgaatc attatgaata attttgtgtt ttttgataaa aaacgatttt gaaagcgact
     5281 gtcaatataa aaacagttat tttattaaaa atttttttac tctaatttta atggaaaaaa
     5341 attcaaaaat atatgtagct ggtcatactg gtttggtagg ttcagccatt aaaagaaatc
     5401 ttgaaaaaaa tgggtttact aacattgtag gtcgtaccca agcagaatta aatttattga
     5461 aagaagctcc tactgaagct ttctttaagg aagaaaaacc agaatatgtt tttctggcag
     5521 cggcaaaagt aggtggcatt gttgctaata atacatttcg agctcaattc atttacgaaa
     5581 atttaatgat acaaaatcat gtcattcatc aaagctattt atatggagta aaaaaacttt
     5641 tattcttggg ctcgtcttgc atttatccac gtttagcacc tcaacccatc aaagaagaat
     5701 atttattaac tggaccgctt gaatatacca atgagcctta tgccatcgca aaaattgcag
     5761 gaattaaaat gtgtgaagca tatcatacac agtatggatg taattttatt tccattatgc
     5821 caaccaattt atacggacca aatgacaatt atgatctgga aaaatcacac gttttacctg
     5881 ctttgattcg aaaaatggtg ttaggaaaat atttaatgga aaatgactgg gaaaatttaa
     5941 gaactgatct aaccagactt cctattgaag gagtgaatgg ttcttatagt aaggaaataa
     6001 tattaaaagt attagaaaaa tatgggatta ttcatcaaaa tgggaaagta gttataacgc
     6061 tttggggaac aggttctcca cttcgggaat ttcttcatgt ggacgacatg gctgatgcca
     6121 ctgtttttct tatgcagcat tatgatgctc ctgatagtat tccttctcac gtaaatgttg
     6181 gttgcggtga agacatttct ataaaagaat tatcagaaat agttaaaaaa attgttgact
     6241 atgaaggtga aattcaatgg gatacaacaa aacctgatgg tacccccaga aa
//
LOCUS       DAOTKS010000039         6288 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_11567_length_6288_cov_4.568266, whole genome shotgun sequence.
ACCESSION   DAOTKS010000039 DAOTKS010000000
VERSION     DAOTKS010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6288
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11567_length_6288_cov_4.568266"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..543)
                     /locus_tag="P5236_01385"
     CDS             complement(115..543)
                     /locus_tag="P5236_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HRU63047.1"
                     /translation="MKSEIRIDKWLWAVRLFKTRTLAAEACKKGKVLIQNVPVKPSHL
                     VKVGDVINIKRNPVLYSYKILKLAENRMNSKLVPDFMTDVTTPDQRELIELGKISQKM
                     GRKPGSGRPTKKERRELDNFIEEPTFWDEEDWDKEENEGK"
     gene            complement(540..1121)
                     /gene="pth"
                     /locus_tag="P5236_01390"
     CDS             complement(540..1121)
                     /gene="pth"
                     /locus_tag="P5236_01390"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRU63048.1"
                     /translation="MKYLIVGLGNIGPEYENTRHNIGFTILDAFAKASNVFFTDKRYG
                     AICEIKLKGHTLILLKPSTFVNRSGLAVRYWMEKEKIEVPNLLVIVDDISLPFGTLRL
                     KPKGADAGHNGLKSIQEILGHNQYARLRVGIGNNYPRGKQVEYVLGHWTEEEANVLPK
                     LVEQAIEMIKSFCLAGIESTMSQFNNKQFLTAQ"
     gene            complement(1252..1824)
                     /locus_tag="P5236_01395"
     CDS             complement(1252..1824)
                     /locus_tag="P5236_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HRU63049.1"
                     /translation="MKTFDLKGTKRTELGKKASKVIRKANEVPCVLYGGKENVFFTVS
                     NKEIQKLIYSPDVFVINLDIDKQKFTSIIQEIQFHPVTDAVLHIDFLEINEKKPVVVK
                     LPVKLEGLAEGVKAGGKLSLELRYLKVKGIYTKLPENITIDVTSLGLGKSIQIGSLSF
                     KDFEILNTKTAIVAQVKTTRAAREAAVPGK"
     gene            2186..2731
                     /gene="infC"
                     /locus_tag="P5236_01400"
     CDS             2186..2731
                     /gene="infC"
                     /locus_tag="P5236_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRU63050.1"
                     /translation="MTLRHQSREAGEYPHRINEEIKGVTQVRLVGDNVQQGIYSIEEA
                     LKIADEQNLDLVEISPKADPPVCKIIDYQKFLYQLKKREKEQKAKTARVIVKEIRFGP
                     QTDDHDYNFKLKHAQEFLKDGCKVKAYVFFKGRSILFKEQGEVLLLRFANDLEDYAKV
                     EHLPELEGKRMTIVLSPKKIK"
     gene            2754..2951
                     /gene="rpmI"
                     /locus_tag="P5236_01405"
     CDS             2754..2951
                     /gene="rpmI"
                     /locus_tag="P5236_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRU63051.1"
                     /translation="MSKTKTNSGAKKRFSITGSGKIKRRHAFKSHLLRKKTKKQKRNL
                     THSALVDKTNLKSVKMLLGLK"
     gene            3045..3395
                     /gene="rplT"
                     /locus_tag="P5236_01410"
     CDS             3045..3395
                     /gene="rplT"
                     /locus_tag="P5236_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRU63052.1"
                     /translation="MPRSVNHVASRKRRKRILNLTKGYFLSRKNVWTVAKNTWEKGLQ
                     YAYRDRKTKKRNFRALWIQRINAAARLEGMSYSKLMGALHKANIEINRKVLADLAMNH
                     PEAFKAIVEKAKNA"
     gene            4319..4921
                     /locus_tag="P5236_01415"
     CDS             4319..4921
                     /locus_tag="P5236_01415"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRU63053.1"
                     /translation="MSTVLYIKANPKAACESKTFKISDKFIETYQKYHPHDEIITLDL
                     YKENIDFLTEEDVKIHENNEVEKKDNPMRKYALQFKNADKYVFAEPMWNLGVPAILKA
                     YIDYISIPNITFGYNENGSVGLCQGKKAVNITTSGGDYSEGIADEWEFCDKYLRAIMA
                     LFGVMDYTAIKAYNLDVMGMDVDAIMNKAINEAEELAKRF"
     gene            5347..>6288
                     /locus_tag="P5236_01420"
     CDS             5347..>6288
                     /locus_tag="P5236_01420"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isomerizing glutamine--fructose-6-phosphate
                     transaminase"
                     /protein_id="HRU63054.1"
                     /translation="MCGIVGYIGDRQAYPILIKGLQRLEYRGYDSAGIALLHDDKLNI
                     YKTKGKVSDLIHYTENKDIEGTIGIAHTRWATHGEPNEINAHPHYSQSKKLALIHNGI
                     IENYSILKEGLTKHGFTFQSETDTEVLVQLIEYMKNKKQVDLSTAVQLALNQVVGAYA
                     IAVIERNHPDVIIAARKSSPLVIGIGENEYFLASDASAIIEYTRKVVYLADEEIVTLE
                     RGKGIKIKTIANVEKTPEVQELEMTLSQLEKGGFPHFMLKEIFEQPKTLSDCMRGRVN
                     VDENNITLSGFIDNKEKFLNAKRIIITACGTSWHAALI"
BASE COUNT         2214 a          962 c         1029 g         2083 t
ORIGIN      
        1 tattcgcaga tattttcatt tttcaattgt tgagcaataa acataattta tttatcaatt
       61 gagaaacctg aataaattaa aaaacactaa cgaattaaga ggaaaattta aattctattt
      121 tccctcattt tcctctttgt cccaatcttc ttcatcccag aaagtgggtt cttcaataaa
      181 attatccagt tcacgacgtt cctttttggt aggtctgccg ctgccgggtt ttctacccat
      241 tttttgagaa atttttccaa gttcaattaa ctctcgttga tctggtgtcg tgacatcggt
      301 cataaaatca ggaactaatt tggaattcat ccgattttca gccagtttaa gtattttata
      361 agaatacaaa acaggatttc ttttgatgtt gattacgtcg cctactttaa ccaaatgcga
      421 gggtttcaca ggcacatttt gaatcagaac ctttcctttt ttgcaagctt cggctgccag
      481 tgttcgtgtt ttgaacagtc gtactgccca gagccattta tctatgcgaa tttcggattt
      541 cattgagcgg ttaaaaactg tttattgttg aattgtgaca tggttgattc aattcccgcc
      601 aaacaaaaac ttttaatcat ttcaatggct tgttctacca attttggtaa tacgttagct
      661 tcttcttctg tccaatgacc cagcacatat tctacttgtt ttccacgagg ataattattc
      721 cctatgccaa cacgcagtcg agcatattga ttgtgtccca atatttcttg aatgcttttt
      781 aagccattgt gaccggcatc agcacctttg ggctttaaac gaagtgtgcc aaatggcaag
      841 gaaatatcat ctacaattac caataaattt ggtacctcaa ttttttcttt ttccatccag
      901 tatcttacag caagaccgct tcggttaaca aaagtagaag gttttaataa aatcaaagta
      961 tgacctttca gttttatttc acagatagct ccgtatcgtt tgtcagtaaa aaaaacattg
     1021 gacgctttcg caaaggcgtc caatattgta aaacctatgt tgtggcgggt attttcatat
     1081 tcaggtccga tatttcctaa ccctacaatt aagtacttca ttccctgaaa aatttatttt
     1141 gaagtaactt tttgaaaaat caataaaaaa attaaagtta agtaaatgct aattatataa
     1201 tttccaattg aaaattttat cacatactta aactttcggt aaaatatttt attatttacc
     1261 aggaactgcc gcttcacgag cagcgcgggt agttttcacc tgagcaacaa tagctgtttt
     1321 ggtatttaag atttcaaaat ctttgaaaga aagagaacca atctgaatag atttacccag
     1381 ccccagtgag gtgacatcaa tagtaatatt ttcaggtagt ttcgtataaa ttccttttac
     1441 tttcaagtaa cgaagttcaa gactcagttt accgcccgct ttgacacctt cagcaagtcc
     1501 ttccaatttt acaggaagtt ttaccacaac tggctttttt tcatttattt ccaaaaaatc
     1561 tatatgcaat actgcatcag taacaggatg aaattgaatt tcctgaataa tggaagtaaa
     1621 tttttgttta tcaatatcca aattaataac aaagacgtct ggcgaataga tcaacttttg
     1681 gatttcctta ttggaaacag taaaaaatac attttctttc ccgccataca aaacgcacgg
     1741 gacttcatta gcttttctta ttactttact tgcttttttc cctaattctg ttctttttgt
     1801 tccttttaaa tcaaatgttt tcattttttt attttatgtg ttactcaaaa tttaagctaa
     1861 ttttagtagt gattagattg cttaatttcc catcacactt tctttttgca aaggtagata
     1921 aaaattttac gttgtacaaa cgtacttaat tttttattgc cttcaattca gagagacagt
     1981 atttttttat attgttaaac aaatggtagc cagataaagt taataaatat ccttattttt
     2041 aagctgaaaa atttataagt gtaaataaat tctttaaaaa aacatagaga attgaaaaaa
     2101 aaattctata tttgcaacct taaaattcaa aaaaatcgat ttgtttaaat tttaaaattg
     2161 aggaggacta aatatagcta aacaaataac tcttcggcat caaagtaggg aagcaggtga
     2221 gtatccccat cgtataaatg aagaaattaa aggagtaact caagtacgtt tggtgggcga
     2281 taatgtccag caagggattt attctattga agaagcttta aaaattgcag atgagcaaaa
     2341 tttagatttg gttgaaatat cgcccaaagc tgatccacct gtgtgtaaaa ttattgatta
     2401 tcagaaattt ctttatcaat taaaaaagcg ggaaaaagaa caaaaagcca agacggcaag
     2461 agttattgta aaagaaattc gttttgggcc gcagactgat gatcatgatt ataattttaa
     2521 attaaagcat gcgcaagaat ttttaaagga cggttgtaaa gtaaaagcgt atgtgttttt
     2581 taaaggacgt tcaatccttt ttaaggaaca gggagaagtt ttgttattac gttttgctaa
     2641 tgatttggaa gattatgcca aagttgagca tttacctgaa ctggagggga aaagaatgac
     2701 cattgtatta tcacctaaaa agataaaata aatagatgta aattttttta aaaatgtcaa
     2761 aaacaaaaac taattctggt gcaaagaaaa gattttccat taccgggagt ggaaaaataa
     2821 aaagaaggca tgcttttaaa agccaccttt taagaaaaaa aacaaagaaa caaaaacgca
     2881 atttaactca ttcagcactg gtggataaaa caaatctgaa aagtgtaaaa atgctgctgg
     2941 ggttaaaata aaaagtttat tttaaaaatt taattattaa ttgaatgcat tagcctaaag
     3001 ttcgtattaa cagtccgacg ctaacattcg taaatttaat tattatgcca agatcggtaa
     3061 atcacgtagc ttcacgtaaa agaagaaaaa gaattttaaa cctgacgaaa ggttattttt
     3121 taagcaggaa aaatgtttgg acagtagcca aaaacacatg ggaaaaaggc ttgcaatatg
     3181 cttatcgcga tcgtaaaacc aaaaaacgga attttcgtgc gttatggatt caacgtatta
     3241 atgccgcagc tcgtcttgag ggtatgtctt attcaaaatt gatgggtgcc ttgcataaag
     3301 ccaatatcga aataaataga aaagttttgg ctgatttagc tatgaatcac cccgaagctt
     3361 ttaaggcaat tgttgagaaa gcaaaaaatg cttgattaat ttctacaaag gtaaaaaata
     3421 gtagtctttt gcataagtta cacctcaaaa ttataaaaaa cttttgaggt gttttttttt
     3481 attcgtttat ttctattttt attgttctca tcgaaagaga caaaaatagt gtttcaaaaa
     3541 atgtgtaaag aataatttta aaaggacttt agagtttgca agttacaagt cacaagagat
     3601 gaaaatttag ttgatagcca gcagcttgga gctttgagtt aatgagacaa ggtgcgagga
     3661 aagaaggatt tttataagga aattctgatt tgtatcaaaa caattgaagc aaagcgaaac
     3721 cgccgaaaca attaaaaata aacacttttg aagcaaaaag ttagtagaat ttggaggtaa
     3781 agcctcaaaa acaaatgttt aattgccgaa gcaaatttta aacagttaaa attttttttc
     3841 tcatgtactt atattttgtt tatagaattg agtaagaatt acttgcgtca attttgaaaa
     3901 acaacctcaa tttttttaat caaaagaatt cctcgaggct tgcctcgggg ttattcatta
     3961 ttttattgat atattatatt gtaattcaaa taattataaa atatttttct cataaccacc
     4021 ctttgaaatt caatcctaat gacaaatgta gattcatttt attgactgct ttttcacaat
     4081 aattgaaatt atcatttttt attatttgaa tttattactg aaaataacaa cccttatttg
     4141 ttttttagca attctttcaa tatgaatgat aaagtgatca ttaagctatg ttagtgtcaa
     4201 gaatttcaaa gaaaaattac tcttcggcat tccttattcc caatattttc tttacttttg
     4261 caaacaaatt ctcaaaaata acaatttgat aataatcact taaaaaggag gtgggtatat
     4321 gagtactgtt ctttatatta aggcaaatcc aaaggcagct tgcgaatcaa aaactttcaa
     4381 aatttctgat aaatttatag aaacttatca aaaatatcat ccccatgatg aaatcattac
     4441 gttggatctt tacaaagaaa atattgattt tttgactgaa gaagatgtga agattcacga
     4501 aaacaatgaa gtggaaaaga aagataatcc gatgagaaaa tatgctttgc agttcaaaaa
     4561 tgcggataaa tacgtttttg ctgaacccat gtggaactta ggggtgccgg ctatccttaa
     4621 ggcatatatt gactatatta gtattcccaa tattacgttt ggatataatg agaatggctc
     4681 tgtgggttta tgtcaaggga aaaaagcagt caatatcact accagcggag gcgattatag
     4741 cgaaggaata gctgatgaat gggaattttg cgacaaatat ttaagagcta ttatggcact
     4801 atttggcgta atggattata ctgccattaa agcttataat ttagacgtca tgggaatgga
     4861 tgttgatgca atcatgaata aggctatcaa cgaagccgaa gaacttgcaa aaagattctg
     4921 aacaatcagt tttttgtatg aatctctcct tcctaaaaag cacaacaaaa tgtgcttttt
     4981 ttattttaaa aaatttataa atcgcttata atcaattaat tgtaatataa atataacttc
     5041 ttcaccattc caataacacc ctatatttaa cttcctacga tctgaaataa tttgataagc
     5101 atttttgctt ttttatagaa aaattctttt atacctcaaa aaaaaatcgc aattttgcat
     5161 tggcttttag aaaaagcagg aatttgacgg gaaagatttt tcaaattaaa atgaatgaaa
     5221 aaataaatta ctgttttctc tgaaatttaa ttttaactct ttctatttta aatcattgta
     5281 aatgaatttc aaaattcctc cctctgtaat aattttctat ttaaaaataa aattaattac
     5341 ttaactatgt gtggaattgt tggatatatt ggtgaccgtc aagcatatcc tattttaata
     5401 aaggggttac aacgattgga atatcggggt tacgacagtg cagggattgc tttgctacat
     5461 gatgataaat tgaatattta taaaacgaaa gggaaagtta gtgatttgat ccattatact
     5521 gaaaataaag acatagaagg aacgattgga attgctcata cccgttgggc gacacatggt
     5581 gaacccaatg aaataaatgc tcatccacat tattcgcaat caaaaaagct cgctttgatt
     5641 cataatggaa ttattgaaaa ttactccatt ctaaaagaag gacttacaaa acatggattt
     5701 acctttcaaa gtgaaacaga cacagaagtt ctggtccaac ttattgaata catgaaaaat
     5761 aaaaagcaag tagatttgtc tactgctgtc caacttgcgt tgaatcaagt agttggtgcc
     5821 tatgccattg cagtaatcga aagaaatcat cctgatgtaa ttattgctgc tcgcaaaagc
     5881 agtccattgg tgattggtat tggcgaaaat gaatattttt tagcatctga tgctagcgct
     5941 attattgaat atacacggaa agtagtttat cttgctgatg aagaaattgt gacactcgaa
     6001 agaggcaagg gaattaaaat taaaaccata gcaaacgttg aaaaaacacc tgaagtacaa
     6061 gaacttgaaa tgacgttaag ccaacttgag aaaggtggat ttcctcactt catgctcaaa
     6121 gaaatttttg aacaacccaa aacactcagt gattgtatgc gtgggcgagt caatgtggac
     6181 gaaaacaata tcactttatc aggctttatt gacaataagg aaaaattttt aaatgctaaa
     6241 cgaatcatta ttactgcttg cggaacttcc tggcatgcag ctttaatt
//
LOCUS       DAOTKS010000040         6282 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_11579_length_6282_cov_4.027943, whole genome shotgun sequence.
ACCESSION   DAOTKS010000040 DAOTKS010000000
VERSION     DAOTKS010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6282
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11579_length_6282_cov_4.027943"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1579)
                     /locus_tag="P5236_01425"
     CDS             complement(<1..1579)
                     /locus_tag="P5236_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRU63055.1"
                     /translation="MEDSERIEVFGAREHNLKNIDVSIPRDALTVITGLSGSGKSSLA
                     FDTIFAEGQRRYMDTFSAYARNFIGNLQRPDVDQITGLSPVISIEQKTTNKNPRSTVG
                     TTTEIYDFFRLLFARASEAYSYVTGEKMVKYSEDQIVDLILKEYNGKKIILLAPVVRG
                     RKGHYKELFEQIRKKGYLHVRIDEKIQDVIPGLKVDRYKNHDIEIVIDRLEIEEKGRR
                     RLKESLQKAMQQGNGIVMILENENQMVRYFSKKLMCPTSGISYDDPDPHHFSFNSPHG
                     ACPYCKGLGFVNEIDLSKVIPNKSLSIYNGGIAPLGKYRNSTIFWQIEAILEKYGCNL
                     KMPIVDIPEEALDEILNGTEEPLYFKNKFAGTTNYFPEYDGIIKYLEMQQEESASATE
                     QKWAHQYSKSVICPECNGARLKKESLHFRIHDKNIAELSEMNISELYQWVNTVEDYLS
                     EKQRKIASEILKEIRNRLQFMLDVGLSYLSLNRSSESLSGGESQRIRLATQIGSQLVN
                     VLYILDEPSIGLHQRDNL"
     gene            complement(1793..3070)
                     /locus_tag="P5236_01430"
     CDS             complement(1793..3070)
                     /locus_tag="P5236_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRU63056.1"
                     /translation="MLMVGAIAYTSAQQAEAPKTPDVSHWSAGIKAGIDYYRVAPGSG
                     VQEYRFFSDKHPSWARYADNAGWMVPEIVVEYTFNPLFGLGFDFAYLTYNRQPSINNK
                     IVGAKGNTLDFTLYGSANLLNLLVPKRGGFWGKVNVYGNLGGGLGFYCYDITNGPTTN
                     DKRAKSPLATAGLNAEYRLSRVLSLGLEGQYRYYVRENLGGQSVNSIGCDALLASIGL
                     RYKFTGKDKTHVRDVSVSDFYPAPAPVVIQKVEQKETAPEVLNRIKAIEDDNATIKAK
                     LQKLESDMQKLSQQPSDVKLPQQPSDVKLSQQPSGVVSATFQNIEFEFGTYNLTKQAT
                     DILDQIYLILQNNPSWTSLTISGHTDNIGSKAGNQKISELRAQAVKDYLVKKGIPASK
                     IKVVGYGGDKPIASNNTPEGRQKNRRVEFEISK"
     gene            complement(3598..>4038)
                     /gene="era"
                     /locus_tag="P5236_01435"
     CDS             complement(3598..>4038)
                     /gene="era"
                     /locus_tag="P5236_01435"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRU63057.1"
                     /translation="VSVLHKFNIEPLKKRIKELLPESPPYFDKDQLTDKPERFFVGEI
                     IREKILLHYEKEIPYSVEVEIEKFTETEKIIHISAIIYVERDSQKGILIGQGGKALKR
                     VGIEARKDIEAFFSKKVFLQLFVKVEKDWRNKEKKLKDFGYQIE"
     assembly_gap    4039..4138
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4347..5501
                     /locus_tag="P5236_01440"
     CDS             4347..5501
                     /locus_tag="P5236_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRU63058.1"
                     /translation="MISLKKIYFIKPRLKSIFIIVAIVIVILSILFTNRLVKSLTLEE
                     HKKMEIWAEALRQFIMADENTNVDFFSNIIANNTTIPVIMTDVNNNLLSSLNVKEPST
                     HVDKFYKKEIERFKRKNPPIEVRLDNTIQYIYYDDSYLLKELRYFPYVQMGVIIIFLI
                     VVYLAFSATKKAEQNQVWVGLSKETAHQLGTPISSLLAWLELLKAKYDDKLLQEMGKD
                     VDRLSIIAERFSKIGSAPELKVVNLKEVVLNATQYISRRTSQKVSIQCHFSGEEPMLI
                     SLNVPLFEWVIENLCKNAVDAMEGNGEIDIYVQKKHNEYVIDVKDTGKGIEKKQFKTI
                     FNTGFTTKERGWGLGLSLAKRIIEEYHQGKIFVKQSEIKKGTVFRIILKA"
     gene            5623..6189
                     /locus_tag="P5236_01445"
     CDS             5623..6189
                     /locus_tag="P5236_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HRU63059.1"
                     /translation="MSKFELYNVNLKDLSNEPQVYHFNLDDAYFQKIDSPEVQRGNIQ
                     ATVTVQKRSTLYHLDFVLDGYVVLQCTRCLEDMNQPIHDKENLDVKLGNKYSEENEVV
                     IVPESEGAINVAWFLYEFVVLNIPIKHVHPPGECNKTMINKLKIHIVHPAEEEDAEND
                     LFEMNDENDFLSEEPTTDFNWDLQNIDN"
BASE COUNT         2080 a          970 c          937 g         2195 t
ORIGIN      
        1 gtaaattatc gcgttgatgt agtccgatac tgggctcgtc caaaatgtac aacacattta
       61 ccagttgcga acctatttgc gttgccaatc ggatacgctg gctctcaccg cctgacaaag
      121 attctgaact ccgattgagg gaaagataac taagcccaac gtctaacata aattgcaaac
      181 ggtttcgaat ttcctttaaa atttcagaag ctatctttct ttgtttttca gaaagataat
      241 cttcgactgt atttacccat tgatataatt ctgaaatatt catttcagat agttcagcaa
      301 tatttttatc atgaattcgg aaatgcaatg attctttttt taatcttgca ccattacatt
      361 ctgggcaaat tactgatttt gaatattgat gagcccattt ttgttctgtt gcagaagcac
      421 tttcttcttg ttgcatttca aggtatttaa taatgccatc atattcagga aaataattgg
      481 tagttcctgc aaatttattt ttaaaataaa gaggttcttc tgttccattt aaaatttcat
      541 caagagcttc ttcaggaata tcaacaatgg gcatctttaa attacaaccg tatttttcga
      601 gtatggcttc gatttgccag aaaatagtag agtttctgta tttcccaagc ggagcaattc
      661 caccattgta aatactaagc gatttattgg gtataacttt actgagatca atttcattta
      721 caaatcctaa tcctttacaa taagggcaag caccatgagg tgaattaaag gaaaaatgat
      781 gcggatcagg atcgtcataa gaaatgccac tggttggaca cattaatttt ttgctgaaat
      841 aacgtaccat ttggttttca ttttccagaa tcataacaat tccattccct tgttgcatgg
      901 ctttttgcaa tgactctttc agacgtcttc gtcctttttc ttcaatttcc agccgatcaa
      961 tgacaatttc tatatcatga tttttgtaac gatctacttt taagccagga attacatctt
     1021 gaattttttc atcaattctt acatgtaaat atcctttttt acggatttgc tcaaataatt
     1081 ctttataatg tccttttcgt cctcgaacaa ctggtgctaa aagaataatt tttttaccat
     1141 tgtattcttt taaaatcaaa tcaacgattt gatcttcaga atacttaacc attttttcgc
     1201 ctgttacata agaataggct tcacttgctc tggcaaacaa caatcgaaag aaatcgtaaa
     1261 tctctgtagt agtaccaaca gtggaacgag gatttttatt tgttgttttt tgttcgatag
     1321 aaataacagg gctcaaccct gtaatttggt caacatcagg acgttgtaaa tttcctataa
     1381 aattacgagc ataagcagaa aaagtatcca tgtatcttcg ttgtccttca gcgaatatag
     1441 tatcgaaagc taaggaagac tttccactcc cactcagtcc tgtaataact gttaaagcat
     1501 ctcgaggaat agagacatca atatttttta aattatgctc tctggcgcca aatacttcaa
     1561 tcctttctga gtcttctata atttttatat tttctttatt gataatcaag gcaataattt
     1621 ttccttaagg gagtaaattt caaataaatt aaattacaaa attacgaaat atcaaagatt
     1681 gtttcaaacg gtgtaaagtt ttatttttaa aaaagcataa agaaaaaggc tgccttttat
     1741 tgaagcagcc tcatctcgaa aataaaaaag aggaaaataa atgaagacta aattatttag
     1801 agatttcaaa ttctactcta cggttctttt gacgaccttc tggtgtgtta ttagaagcaa
     1861 tcggtttatc tccaccataa ccaacaactt tgattttaga agcgggaata ccttttttta
     1921 ccaaataatc tttaactgct tgagcacgta attctgaaat tttttgattg cctgcttttg
     1981 atccaatgtt atcagtatgt ccagaaattg tgaggcttgt ccaggaagga ttattttgta
     2041 gtattaaata aatttggtct aaaatatcag ttgcttgttt tgttaaattg taagtaccaa
     2101 attcaaattc aatattttgg aatgttgcac ttactacgcc tgaaggttgc tgtgataatt
     2161 ttacatctga aggttgctgt ggtaatttta catctgaagg ttgctgtgat aatttttgca
     2221 tatcactttc caacttttgt agtttagctt tgatagttgc attatcatct tctatcgcct
     2281 tgattcgatt taacacttca ggagctgttt ctttctgctc aactttttgt attactactg
     2341 gtgccggtgc agggtaaaaa tcgcttacac taacatctct aacatgtgtt ttatcttttc
     2401 ctgtaaattt ataacgaagt ccaattgaag ctaataaagc atcacaacca attgaattaa
     2461 cactttgccc gcctaagttt tctctaacgt agtagcgata ttggccttca agacctaatg
     2521 ataacaccct acttaaacga tattcagcat ttaatcctgc tgttgctaac ggagattttg
     2581 cacgtttatc attagttgtg ggaccatttg ttatatcata acaataaaaa cctaaccctc
     2641 cacctaaatt tccgtaaaca ttaacttttc cccaaaaacc tccacgttta ggaacaagta
     2701 aattcagtaa atttgcagaa ccgtacagag taaaatcaag agtattacct tttgctccaa
     2761 ctattttatt atttattgat ggctgcctat tataagttaa gtaggcaaaa tcaaatccta
     2821 atccaaatag cggattaaaa gtatattcaa caacaatttc gggaaccatc caaccagcgt
     2881 tatcagcgta acgagcccat gagggatgtt tgtcactaaa aaacctatat tcttgtaccc
     2941 cacttccagg agcaacacga taataatcga ttccagcttt tatgccagca gaccaatgtg
     3001 atacatcagg tgttttcgga gcctctgctt gttgtgcaga tgtataagca atggcaccta
     3061 ccattaacat aacactcaat aaaatttttt tcataagctt agtggattta taaattttgt
     3121 tcaacttaaa ttttatttag ctaaaaaatg tttttataaa ggtataattt tttataaata
     3181 atattttcat ttctttcttt cacaaaaagt ttaaagtaac ttttgcaaat atatatataa
     3241 aaaaagtaat tatacaaatt ttttctatct ttttttataa attttttgtt tcactttatt
     3301 aatttttata ttttttattt tttagagatt aacaataatt ataccaaaaa cagtacaatt
     3361 aataaaatta agaggaaaag atttaaatat tttttatttc ttactttaat gatcaaagta
     3421 aatcatttat aatgagcaat ttattttgaa agctttaaaa ttatagcctt gttatatcaa
     3481 tttttccaat atggaagttt taatacagtt atttttcttc ataaggttaa aattttaatc
     3541 aaaatttttt cgagaaaatt ttaaaatagt aatttaattc ataaaaattg tctaaattta
     3601 ttcaatttga taaccaaaat cttttaattt tttttctttg tttcgccagt ctttctcaac
     3661 ttttacaaat aattgaagga atactttttt tgaaaaaaac gcttcaatat cttttctcgc
     3721 ttctatcccg actcttttta atgcttttcc cccctgacct ataagaatcc ctttctgaga
     3781 atcacgttct acataaataa tagcacttat gtgaataatt ttttcagttt ctgtgaattt
     3841 ttcgatctcg acttctacgg aatagggaat ttctttttca tagtgaagca gaattttctc
     3901 acgaataatt tcacctacaa aaaaacgttc tggtttatca gtcaattgat ctttatcaaa
     3961 ataaggtggt gattcgggta ataattcttt tattcttttt ttcaaaggtt caatgttaaa
     4021 tttatgaagc accgacacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca
     4141 aggaagattt tcctacattt gggttgccta ttacatttac aaatcctgct ctatgcataa
     4201 aaaatagttt taagattttg cttgactatt aatgactttt caaccttcaa agatatgtat
     4261 aaataatgta gaattaaaaa ttctcgaaaa ttttttatta tttttgaaac ttcaattgct
     4321 aactgaatct ggttataaat tttattatga tctcacttaa aaagatttat ttcatcaaac
     4381 cacgtttaaa atcaattttt attattgttg ccattgttat tgtgattctt tccattttgt
     4441 ttactaacag actggttaaa tcattgacgt tggaagaaca taaaaaaatg gaaatatggg
     4501 cagaagcatt gcggcaattc atcatggcag atgaaaatac aaatgttgat tttttttcaa
     4561 atattattgc taataatact actattccag taattatgac tgatgtcaat aacaatttac
     4621 tgtcttcgtt gaatgtaaaa gaaccttcta cccatgttga caagttttac aaaaaagaaa
     4681 ttgaacgttt caaaaggaag aatccgccca ttgaagttcg tcttgataat acgattcaat
     4741 atatttatta tgatgactcc tatttgttga aagaattacg atatttccct tatgttcaga
     4801 tgggtgttat cattattttt ctgatcgtgg tttatctggc tttttctgca accaagaaag
     4861 ctgaacaaaa tcaagtttgg gtagggcttt cgaaggaaac agcccatcaa ttgggaacac
     4921 caatttcatc tttattagct tggcttgagt tgctaaaagc aaaatatgat gataaattgt
     4981 tacaagaaat gggaaaagat gttgaccgtc tgtctatcat agcagaaaga ttttcaaaaa
     5041 taggttctgc acctgagttg aaagtagtaa atttgaaaga agttgtacta aatgcaaccc
     5101 agtatataag cagacgtact tctcagaaag ttagcattca gtgtcatttt tcgggtgaag
     5161 aacctatgtt gatatcatta aatgttccac tttttgaatg ggtaattgag aatttatgta
     5221 aaaatgcagt tgatgcaatg gaaggaaatg gagaaattga tatatatgtt cagaaaaagc
     5281 ataatgaata tgtcattgat gttaaagata caggaaaagg aattgaaaag aaacagttca
     5341 aaactatttt caatacggga tttacaacta aagaacgtgg atggggattg ggcctttcgt
     5401 tagcaaaacg catcattgaa gaatatcatc agggtaaaat ttttgttaaa caatctgaaa
     5461 taaaaaaagg aaccgtattt agaataattt taaaggcata agtagaggaa atttcattaa
     5521 ccagatgact cgaaggcttt ataataaaat ataaaattaa attattttac aattgcagag
     5581 atttttatac ctttgtaccg tttttttaat tgctaataag ccatgtcaaa atttgaatta
     5641 tataatgtca atttaaagga tttatccaat gaacctcaag tttatcattt taatttagac
     5701 gatgcttatt ttcaaaaaat tgatagtcct gaagttcaaa gaggcaatat tcaagcaaca
     5761 gttactgttc agaaaagatc aacactttat catcttgatt ttgttcttga tggttatgtt
     5821 gtgttgcaat gcacacgttg cttggaggat atgaatcaac ctattcatga caaggaaaat
     5881 ttagatgtaa agttaggaaa taaatattct gaagaaaacg aagtagttat tgtgcctgaa
     5941 tcagaaggtg caattaatgt agcatggttt ttatatgaat ttgtggtatt gaatattccc
     6001 attaagcatg ttcatccgcc aggtgaatgt aacaagacaa tgataaataa actaaaaatt
     6061 catatagttc atcctgccga agaagaagat gctgagaatg acttatttga gatgaatgat
     6121 gagaatgatt tcttatcgga ggaaccaact acagatttta attgggattt acaaaacatt
     6181 gataattgaa taaataaata aataatagaa aaaatggaac atccaaaaag aaaacattca
     6241 aaacaacgta ctgctaagag aagaacacat gataaagaaa aa
//
LOCUS       DAOTKS010000041         6263 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_11607_length_6263_cov_3.978576, whole genome shotgun sequence.
ACCESSION   DAOTKS010000041 DAOTKS010000000
VERSION     DAOTKS010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6263
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11607_length_6263_cov_3.978576"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /gene="rsmH"
                     /locus_tag="P5236_01450"
     CDS             <1..509
                     /gene="rsmH"
                     /locus_tag="P5236_01450"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRU63060.1"
                     /translation="NTYSEEKLADIFFNYGELRNARKIAKIIVENRDKQPLIQISSFV
                     ELIKPLFAKEKGKKDLARVFQALRIEVNHELEALQAFLQQSLQVLQPQGRLVIITYHS
                     LEDRMVKNFLKSGNFAGKIEKDFYGHPFLPFQLINNKVIVPNNNEIEQNPRARSAKLR
                     IAERKSLI"
     gene            544..924
                     /locus_tag="P5236_01455"
     CDS             544..924
                     /locus_tag="P5236_01455"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HRU63061.1"
                     /translation="MSVFTKFFKSIIREEDWAEIKSSSFRDILNGNIFKKRFFTRQLG
                     LIAMIVVLLIIYIGNRYSYEKQLVYQNKLKKEIQDKKYESLTISAQLMQISRQSNVLK
                     MLNEKGIDLHELNEPLIILNDSVN"
     gene            927..>2773
                     /locus_tag="P5236_01460"
     CDS             927..>2773
                     /locus_tag="P5236_01460"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRU63062.1"
                     /translation="MANNRKNIILRFALVYFLIVLAFLLVIYKIIVIQFVERDHWLAL
                     AAKNVKTNIVIKPNRGNIYACDGSLMASSIPTYYVYMDLRVPALHENDGALFKENIDS
                     VAICLSKFFGDKSVAQYKNDITKAYFEEKGEYRLYPKRISYAQLKILKTFPLFRLGKN
                     KSGLVTKELFERVKPFGSLASRTIGDIYADESKGGKNGIELAFDSVLRGTPGVGVRQK
                     IANSWQETVQVEPVDGKDVITTINIDFQDIAEKVLLDSLKAFDAEAGYVVLMEVQSGE
                     VKAIVNMQKNADGTYSENKNGVVSDLVEPGSTFKVVSLVAALDDGKVKITDTIDTGNG
                     IYQYANSFMKDHNYSRGGFHKITIAEAIQASSNIGISRMIVNAYGDNPAAYVNKLYQM
                     KLNVPMQLEIPGTAAPQIRHPLDKTRYWSKTTLPWMSIGYEVQIPPIYTLAFYNAIAN
                     NGKLIRPFFVKAISKNGQVVKTFQTETINHSICKPEVLKIVQETLLGVIEGKLGTAKN
                     VRSKYFRIAGKTGTAQISKGALGYKSGGKAHQVSFCGYFPAEHPQYTCIVVIREPHIG
                     YPSGGKMSGSVFKNIAERVMFLKSNAKPENLEVDTLSHKPKLPYCFSGR"
     assembly_gap    2774..2873
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2874..3217
                     /locus_tag="P5236_01465"
     CDS             <2874..3217
                     /locus_tag="P5236_01465"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HRU63063.1"
                     /translation="VDTLSHKPKLPYCFSGRFCDLQTVASQMQLSVIGSPSDYVRVNT
                     ETQLRIEPINIMHNIMPDLINMGAKDAVYLAEKLGLKVQINGYGKLISQSISAGSPIQ
                     KGQSVLLSFQE"
     gene            3240..4694
                     /locus_tag="P5236_01470"
     CDS             3240..4694
                     /locus_tag="P5236_01470"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018920551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRU63064.1"
                     /translation="MTLNNLLQGIPLLEVIGDMNINISSIQFDSRKIQTGNVFVAIRG
                     TSVDGHQFIPSAIEKGAVAIVCEEYPVKLNPNVTYVKVENSAEVLGKIACAWFGFPSS
                     KLILVGVTGTNGKTTVATLLYQTFRKLGCKAGLLSTVRNYIDEIPMEVTHTTPDPLEL
                     NEYLAKMVEAGCQYAFMEVSSHSIDQRRIAGLEFKGGIFTNLTRDHIDYHLTFENYLK
                     AKKQFFDDLPSTAFALTNLDDKNGLVMVQNTKAPIHTYSLYHLADFKTKIIEQSWEGM
                     LLEMNGKQVHVNFVGRFNASNLTAIYGGAVLLGAEEMEVLRIISTLHPVSGRFETLHS
                     PTGFTVIVDYAHTPDALKNVIETIHDILQGKGRLITVVGCGGNRDKGKRPLMAQEVVK
                     GSWKAILTSDNPRFEDPMEILNDMLAGLTSEEKSQCLTIVDRKEAIKMACALAQPGDV
                     VLIAGKGHEDYQIVQGKKQHFDDKEEVKKCFKIA"
     gene            4768..6030
                     /gene="mraY"
                     /locus_tag="P5236_01475"
     CDS             4768..6030
                     /gene="mraY"
                     /locus_tag="P5236_01475"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRU63065.1"
                     /translation="MLYHLFQYLDKTFNVPGAGMFNYLSFKSALAFILSLFISAYFGK
                     RIIAFLQKKQIGEPIRDLGLAGQMDKKGTPTMGGIIIIISIVIPTLLFANLTNIYIIL
                     MLITTLWIGIIGFLDDYIKVFLKNKKGLSGKFKIVGQVGLGLIVGLIMYLSPDIVIRE
                     NTEIKGELRVESVIYSKKDIKSTKTTIPFVKNNDLDYADAFKKAGENAQKYGWLLFVL
                     VAIFVVAAVSNSANLTDGLDGLSAGSSAIMGVTLGILAYLSGNINYAGYLNIMYIPGV
                     GELLVFAAAFIGATIGFLWYNSYPAQVFMGDTGSLTLGGIIAVFALAIRKELLIPILC
                     GIFFAESLSVIFQVGYFKYTKKRFGEGRRIFKMTPLHHHFQIGSDAGIKALIQKPYQP
                     LHESKIVVRFWIVGTILAAFTIITLKIR"
     gene            6041..>6263
                     /locus_tag="P5236_01480"
     CDS             6041..>6263
                     /locus_tag="P5236_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRU63066.1"
                     /translation="MKRMVILGGGESGTGAAILAKKQNFDVFLSDNSEIKENYKQLLN
                     QYDILWEEKHHTEELILTADEVVKSPGIPD"
BASE COUNT         2081 a          842 c         1186 g         2054 t
ORIGIN      
        1 tgaacaccta ttctgaagaa aaattggctg atattttttt taattatgga gaattgagaa
       61 atgccagaaa aattgctaaa ataattgttg aaaatcgtga taaacaacct ttaatacaaa
      121 tttcttcttt tgtggaatta ataaaaccgt tgtttgctaa agaaaaagga aaaaaagatc
      181 tggcgcgtgt tttccaggct ttgcgtatag aagtaaacca tgaattggaa gcgttacaag
      241 cttttttaca acaatcatta caagtgcttc aaccgcaagg tcgtctggtc atcattactt
      301 atcattcatt ggaagacaga atggtaaaaa attttctaaa aagtggtaat tttgcaggaa
      361 aaattgaaaa agatttttat ggtcatccct ttttaccttt tcagttgatt aacaataaag
      421 taattgttcc taataataat gaaattgaac aaaatccaag agctcgtagt gccaaattaa
      481 gaatagccga acgaaaatct ttgatttaaa aatggttttt tacaactttt taaattcacc
      541 aaaatgtctg tttttacaaa attttttaaa agtattattc gtgaagaaga ttgggcagaa
      601 attaagtcca gttcttttcg ggatatttta aacggcaata tttttaaaaa acgttttttt
      661 acaaggcaac tcggcttaat tgctatgatt gtagttctgc tgattattta cattgggaat
      721 cgatatagtt atgaaaaaca gttggtatat caaaataaat taaaaaaaga aattcaagat
      781 aaaaaatatg aatcattaac tatttctgct caattaatgc aaattagccg tcaatcaaat
      841 gtattgaaaa tgttgaacga aaaaggaata gaccttcatg aactaaatga accattgatt
      901 atattaaatg actcagtcaa ctaaaaatgg ctaacaatcg taaaaatatt atccttcgtt
      961 ttgctctggt atatttttta atagtgcttg cttttttatt ggtgatatat aaaattattg
     1021 taattcagtt tgttgaacgt gatcattggc tggcattagc agcgaaaaat gtaaaaacca
     1081 atattgttat aaaaccaaac agaggaaaca tttatgcttg cgatggaagt ttgatggcaa
     1141 gttctattcc tacttattac gtttatatgg atttgcgagt tccagcttta catgaaaatg
     1201 acggagcact ttttaaggaa aatattgatt ctgttgctat ctgtttgtct aaattttttg
     1261 gcgataaatc tgttgctcaa tacaagaatg atataacaaa agcttatttt gaagaaaaag
     1321 gcgaatatcg tttgtatccc aagcgcattt cttatgctca attgaaaata ttgaaaactt
     1381 ttcctctttt tagattagga aaaaacaaaa gtggtttagt tacaaaggaa ttatttgagc
     1441 gagtgaaacc ctttggttct ctggcttcac gaaccatagg agatatttat gctgatgaat
     1501 caaaaggtgg aaaaaatgga attgaattgg cttttgattc tgtgttaaga gggacgccag
     1561 gtgtgggcgt gagacaaaaa attgcaaatt cttggcagga aactgttcaa gtagaacctg
     1621 ttgatggaaa ggatgttatt accactatta acattgattt tcaagatatt gcagaaaaag
     1681 ttttattgga tagtttaaaa gcttttgatg cagaggctgg ttatgtagtg ttaatggaag
     1741 ttcaatcagg agaagtaaaa gccattgtaa atatgcaaaa aaatgctgat ggtacttatt
     1801 ctgaaaataa aaatggtgta gtatcagatt tagtagaacc aggttctact tttaaggtag
     1861 tatctttagt agcagctttg gacgatggaa aagttaaaat tacggatacc attgatactg
     1921 gtaatggcat ttaccaatat gccaattctt ttatgaagga tcacaattat agtcgaggag
     1981 gatttcataa aataaccatt gcagaagcca tccaagcttc atccaatatt ggaatttcca
     2041 gaatgattgt caatgcttat ggtgacaatc ctgcagctta tgtaaataaa ttgtatcaga
     2101 tgaaattgaa tgtgccgatg cagttggaaa tccctggtac agcagcacca caaatcaggc
     2161 atccactaga caaaacaagg tattggtcaa aaactacttt accgtggatg tccattggct
     2221 atgaagttca aattccgccg atttatacat tggcttttta taatgctatt gctaataatg
     2281 gaaaattaat ccgtccgttt tttgtaaagg cgatcagtaa aaatggacag gtagttaaaa
     2341 cctttcaaac cgaaactatt aatcactcca tatgtaaacc tgaagtactt aaaattgttc
     2401 aagaaacttt attgggtgta atagaaggta aattaggaac agcaaaaaat gtgagatcaa
     2461 aatattttcg tattgctggc aaaactggta cagctcaaat atcaaaagga gcattgggat
     2521 ataaatctgg aggaaaggca catcaggtat cattttgtgg ctatttccct gctgaacatc
     2581 cacaatatac ctgcatcgtt gtaattcgtg aacctcacat tggctatcca tctgggggaa
     2641 aaatgagtgg ttcagttttt aaaaatattg cagaacgtgt gatgttttta aaatcaaatg
     2701 caaaacctga gaatttggaa gttgatactt tgtcacataa gccaaagtta ccttattgtt
     2761 tttcaggaag attnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaagttga
     2881 tactttgtca cataagccaa agttacctta ttgtttttca ggaagatttt gcgacttaca
     2941 aacagttgct tctcaaatgc agctttcagt gattggttca ccaagcgatt atgtaagagt
     3001 aaatactgaa acccagttaa ggattgagcc tattaatatt atgcataata taatgcccga
     3061 tttaatcaat atgggagcta aagatgctgt ctatcttgct gaaaaattag gcttgaaagt
     3121 acaaatcaac gggtatggaa aattgatttc ccagagcatt tcagccggct cgccgattca
     3181 aaaagggcaa tcagttttat tgagttttca agaataatga ttttaataaa ttagatgtta
     3241 tgactttaaa taatctttta cagggaattc ctcttttaga agtgataggc gatatgaata
     3301 tcaatatttc gtctattcag tttgattcac gtaaaattca aacagggaat gtttttgttg
     3361 ctatacgtgg tacaagtgtt gatgggcatc aatttattcc gtcagccatt gaaaaggggg
     3421 cagtggcaat tgtttgcgaa gaatatcctg taaaattaaa tccgaatgtg acatatgtaa
     3481 aagtcgaaaa cagtgctgaa gttttgggaa aaatagcttg tgcctggttt ggctttcctt
     3541 cttcgaaatt gatattggtg ggtgtgactg gaactaatgg aaaaactact gttgctactc
     3601 ttttatatca aacttttcgt aaattgggat gtaaagcagg gttattgtcc actgtccgaa
     3661 attatattga tgaaatacca atggaagtaa cccacacaac ccctgatcca cttgaattga
     3721 atgaatattt ggcaaaaatg gtagaagcag gttgtcaata tgcttttatg gaagtaagtt
     3781 cgcactccat tgatcagcgg cgtattgctg ggctggaatt taaaggaggt atttttacca
     3841 atctcacacg tgatcacatt gattatcatt tgacttttga aaattatttg aaagcaaaaa
     3901 aacaattttt tgatgattta ccttctactg cttttgctct cactaattta gatgataaaa
     3961 atggactggt aatggttcaa aatacaaaag ctccgattca cacttattca ctttatcatt
     4021 tggctgattt taaaactaaa attatcgagc aaagttggga aggaatgttg cttgaaatga
     4081 atggtaaaca agtacatgta aattttgttg gcagattcaa tgccagtaat ttaacagcaa
     4141 tttatggagg agcagtatta ttgggagccg aggaaatgga agttctaaga ataatcagta
     4201 cacttcatcc tgtttcaggt cgttttgaaa cattacattc tccaacagga tttacagtta
     4261 ttgtagatta tgctcatacg cctgatgctt taaaaaatgt gatagaaacc attcatgata
     4321 ttttgcaagg taaaggcagg ttaataacag tagttggttg tggtggcaat cgtgacaaag
     4381 ggaaacgtcc tctcatggct caagaagtgg taaaaggtag ttggaaagcg attctcacat
     4441 ctgataatcc tcgttttgaa gatccaatgg aaattttgaa tgatatgtta gctggtttga
     4501 cttctgaaga aaaatcacaa tgtttaacta ttgttgatcg aaaagaagca attaaaatgg
     4561 cttgtgctct tgctcaacca ggtgatgtgg ttttaattgc agggaaaggt cacgaagatt
     4621 atcagattgt tcagggtaaa aaacaacatt ttgatgataa agaagaggtt aaaaaatgtt
     4681 ttaaaatagc ttgattactt gtcgattttt aaagataaaa atatggggct ctcaaataca
     4741 aatgcttaat ttaaaaacaa ttataggatg ttgtaccatt tatttcaata tttagataaa
     4801 acatttaatg tgccaggtgc aggaatgttt aattatcttt cgtttaaatc tgcccttgct
     4861 ttcattctgt ctctttttat ttctgcttat tttgggaaac gaattattgc tttcttgcaa
     4921 aaaaaacaaa ttggtgaacc tattcgggat ttaggattag ctggacaaat ggataaaaaa
     4981 ggtactccta caatgggtgg aattatcatt attatttcta ttgtgattcc aactttactc
     5041 tttgctaatt tgactaatat ctatattatt ttaatgttaa taactacttt atggattgga
     5101 atcataggtt ttttagatga ttatataaaa gtttttctga aaaataaaaa aggtttaagt
     5161 ggaaaattta aaattgttgg acaggtaggt ttaggattga tagttggttt aattatgtat
     5221 ttgagccctg atattgtaat tcgtgaaaat actgagatca aaggagaatt aagggtagaa
     5281 agcgttatat attcaaaaaa ggatattaaa tcaacaaaaa caactattcc ttttgtaaaa
     5341 aataatgatc tcgattatgc tgatgctttt aaaaaagcag gagaaaatgc tcaaaaatat
     5401 ggttggttgt tatttgtttt agttgccatt tttgttgtgg ctgctgtatc aaatagtgcc
     5461 aatttaacag atggactgga tggtttaagt gcaggttctt cagcgattat gggggttaca
     5521 ttgggaattt tagcttatct ctcaggaaat attaattatg ctggttattt gaatattatg
     5581 tatattcctg gggtagggga attgttggtt tttgctgcag cttttatcgg agcaactatt
     5641 ggttttttgt ggtacaactc ttatccggca caagttttta tgggcgacac tggaagttta
     5701 acattgggtg gaattatagc tgtttttgct ttggcaatcc gtaaagaatt attgatccct
     5761 attttgtgcg ggatattttt cgctgaaagt ttatcggtca tttttcaggt aggttatttt
     5821 aaatatacaa aaaaacgttt tggagaaggc agaagaattt ttaagatgac acccttgcat
     5881 catcattttc aaataggcag tgatgcaggc attaaagctc tcattcagaa accttatcaa
     5941 ccgcttcacg aatcaaaaat tgtagttcgt ttctggattg tgggaactat tttggcggca
     6001 tttaccataa ttacattaaa aataagataa atcatttttt atgaaacgta tggttatatt
     6061 gggtggtggt gaaagcggaa caggagctgc tatactggca aaaaaacaaa attttgatgt
     6121 ttttctttca gataattctg aaataaaaga aaattataaa caattactta atcaatatga
     6181 tattttatgg gaagaaaaac atcatacaga agagttgata cttactgccg atgaagtggt
     6241 gaaaagtcca ggaattccag ata
//
LOCUS       DAOTKS010000042         6247 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_11635_length_6247_cov_4.739503, whole genome shotgun sequence.
ACCESSION   DAOTKS010000042 DAOTKS010000000
VERSION     DAOTKS010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6247
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11635_length_6247_cov_4.739503"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..2079)
                     /locus_tag="P5236_01485"
     CDS             complement(25..2079)
                     /locus_tag="P5236_01485"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRU63067.1"
                     /translation="MDFLYRTPFVRLLFTLIIGILIYQYIELYTFTRYAIYLIATFLI
                     ISPFFIQDAKNQFHFRWLFGVGTFLIFAMLGYSLSAQHEKSTSFSPLNKKGIYIVELT
                     SAPTEKAKSFFYSTRVKQFLNDDRWETAHGKAYLYLQKDSAASSLIVGDQLLIETEFK
                     APEKALNPDEFNYAAYLKRQGVGATAYVPTGYWKKTGENHSFSLIRFSDQCRNYLFKI
                     YQKFNIQGDEFSVLAALTLGYKDDLNPDLKKSYSASGAMHILSVSGLHVGTVYAVLLF
                     LFGFLQNKKPQKLLKSILLILSLWGYAFITGLSPSVVRATLMFTFVAVATCLDRKSEI
                     YNTICMSAFFMLLVNPNYLFDIGFQLSYAAVFSIIYFQPYFTIFIPVSNKILKSLRDL
                     LTVSVAAQIGTAPFTLYYFQQFPNYFLFTNLIVIPLSTIIIYLAVGLFAISFVPYVSN
                     LVAFLLNISVKLLNFCIVKIEHFPYSISQVSLDSKQSFFIILFIFCISAYFYYKKYTP
                     LIMGLLSILFIISIDFYARYQTLNTRRMIVYAGQRNTHVNFIDQGNNFLFSTDEKEAE
                     RVATSYWRKHKLKPPIEVQNTSWFTNGFACFQGQRIFILINDELKNKIAQKPLEVDYL
                     IIGNGQKIRMEQLLSCIHPRKVIVDKTISKWYADNIQQTCEKQKIEFYSIANEGAFIL
                     NF"
     gene            complement(2106..2759)
                     /gene="rpe"
                     /locus_tag="P5236_01490"
     CDS             complement(2106..2759)
                     /gene="rpe"
                     /locus_tag="P5236_01490"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRU63068.1"
                     /translation="MGNLISPSILTADFSNLQAICELINQSEADWIHLDVMDGVFVPN
                     ISFGFPVIKAVKKHCKKPLDVHIMIIHPEKYVQRFAEAGAGILTFHYEAAEHPAEIIE
                     QIHAAGMKAGITINPDIPVKVLEPFIEKVDMVLLMSVFAGYGGQKFIEETYQRINELK
                     SIIQQKNPSCLLEVDGGINLENAPKLFAQGVDVLVVGNTVFNSNQPLEIIHQLKTIA"
     gene            3232..4026
                     /locus_tag="P5236_01495"
     CDS             3232..4026
                     /locus_tag="P5236_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005803499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRU63069.1"
                     /translation="MTQNYLKALSKNKIKLISRLQQKKWRDELGCFLAEGTKLVTDLS
                     TSFKGMLLVATPDWLKNHLPIQAEETLIVTSEEFKKISTQKTPQGVLAIFKKKETKWS
                     FLEISSQLCFALEDIQDPGNLGTIIRVADWFGISNIFCSEHSADVYGTKVVQATMGAL
                     ARVQVHIVHLPDFLIESKKYQIPLYGTFLKGENIFTTSLTPEGIIVLGNEGNGISSVL
                     ENIIPTRLTIPSYPSENFTSESLNVSVAAGIVAAEFRKRLMIHKGI"
     gene            complement(4049..>6247)
                     /locus_tag="P5236_01500"
     CDS             complement(4049..>6247)
                     /locus_tag="P5236_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRU63070.1"
                     /translation="KQINIDKSSIDLGIISLKEETKFLSEVEVVAQGFQMKFDIDKKV
                     FSVDQNIASAGGSATEVLQNIPSVDVDNEGNISLRNNSNVEVWINGKPAGLTTENRAQ
                     VLQQMPAESIESIEIITNPSAKFDPEGTAGIINLVLKKNRKAGYYGSLSAGLMYPENA
                     KLGKTLGGNFNYSSSKVDAYANIGYRSMNMKGGGWNRRYSYLAMDTTLLSQNNAMNHS
                     FDGLFLRAGADFHLTDKNSIGISGFSMYQKENSSNFLDYLLTSPIYQYTIRDYSRNTF
                     EKGTHPSYNLTLDYQHDFDKKGTNLIASLTYSNHDRNSEKQFIQREDSLEQATSDITQ
                     NSDNQNNNWNFKVDYTNKFTENNKLEAGWQSRIQSRNSLVSGVDNLQQGANIPSYYNN
                     FDYDEQIHAAYVTYGGRFNRLSLQGGVRAEYMWKQITNETYDSIAQLDPQTYFQLFPS
                     IYISYTLPQNGEIQLNYTRRVDRPRGRMINPFRDYTDSTNISFGNPELKPQFSSSYEL
                     NYLKNWYYHSLSFSAYYRSRNNVIQNVRYMHGNNMENTYLNITKTQNSGLEVVVKDNF
                     SNVLNLTSSLNLYFNKLDSATYISPYNTICHIPAQENFSWNARVIANVLLAKTFSAQI
                     SGNYSSPTIISQGKRKERYSIDLGLRKTFMDRKLNLSLMVRDLLNSRKMRSTTWGEDF
                     YQQTESYFHGRMVGLTISYNFGNMKPTSQKKKSTENNNGEEFPMDNFEGL"
BASE COUNT         2166 a         1030 c          972 g         2079 t
ORIGIN      
        1 aaaacgggaa tgccccaaaa aacactaaaa atttaaaata aaagctcctt cattggctat
       61 ggaataaaat tcaatttttt gtttttcaca agtttgttga atgttgtcag cgtaccattt
      121 tgaaatggtt ttatctacaa ttactttgcg aggatgaatg cacgataaaa gttgttccat
      181 acgtattttt tgtccattcc caataattag ataatctact tcgagaggtt tttgggctat
      241 tttatttttt aattcatcat taataagaat aaatatgcgt tgcccctgaa aacacgcaaa
      301 gccatttgta aaccaggaag tattttgtac ttcgataggg ggttttagtt tgtgttttct
      361 ccaataagaa gtagccacac gttctgcttc tttttcatca gtagaaaaaa gaaaattatt
      421 tccttgatca ataaaattta catgagtatt tctttgtcca gcatatacaa tcattcgacg
      481 ggtatttaag gtttgatatc gagcataaaa atcgatagaa ataataaaca agatagacaa
      541 caatcccatt attaaaggcg tatatttttt ataatagaaa taagccgaga tgcaaaaaat
      601 gaaaagaata ataaaaaaac tttgtttgct atccaatgaa acttgagaaa tagaataagg
      661 aaaatgttca atttttacaa tgcaaaaatt gaggagttta acagaaatat ttaataaaaa
      721 agctacaaga ttagaaacat aaggaacaaa tgaaatagcg aaaagtccaa cagcaaggta
      781 aataataata gtggacagtg gaattactat taaattggta aacagaaaat aattaggaaa
      841 ctgttgaaag tagtataacg taaaaggtgc ggtgccaatt tgtgcagcta ctgaaacagt
      901 aagtaaatca cgcaaacttt tcagtatttt attgctaaca ggaataaaaa tagtaaaata
      961 aggttgaaaa tagataatac tgaataccgc tgcataacta agttggaaac caatatcaaa
     1021 taaataattg gggttcacca gcaacatgaa aaaagccgac atgcaaatag tgttgtaaat
     1081 ttcagatttg cggtcaaggc aggtagctac tgctacaaaa gtaaacatca aagtagctcg
     1141 aaccactgaa ggagataatc ctgtaataaa agcatatccc cacaatgata aaatgagaag
     1201 gatacttttt agcaactttt gtggcttttt attttgtaaa aaaccaaaca gaaaaagcaa
     1261 caccgcataa accgttccca catgcaaacc acttacagat aatatgtgca ttgctcctga
     1321 cgcactataa ctttttttca aatcaggatt caagtcgtct ttgtatccta aagtcaatgc
     1381 tgccaacaca ctgaattcat caccctgaat attaaatttt tgatagattt taaacagata
     1441 atttctacat tgatctgaaa aacgaatgag cgagaaggaa tgattttcac ctgttttttt
     1501 ccaataacca gttggaacgt aagcagttgc gccaacgcct tgcctcttta aataggctgc
     1561 ataattaaat tcgtcaggat tcaaagcttt ttctggagct ttaaattcag tctctatcaa
     1621 taactggtcg ccaacaatca gagacgaagc cgcactatct ttctgaagat acagataagc
     1681 tttgccgtga gcagtttccc agcgatcatc gtttaaaaac tgttttactc gagtagaata
     1741 aaagaatgat tttgcttttt ctgtgggagc tgaagttaat tcaacaatat aaattccttt
     1801 tttatttaag ggagaaaatg aagttgattt ttcatgttgg gcacttaatg aatagcccag
     1861 catggcaaaa attaaaaaag tacctacgcc aaatagccat cgaaaatgaa attgattttt
     1921 tgcatcttga ataaaaaaag gggaaatgat cagaaaagta gcaattaaat aaatggcata
     1981 tcttgtaaat gtatataatt caatgtattg atagataaga attccaatta tcagagtaaa
     2041 caacaatctt acaaaaggag tacgatacaa aaaatccata catcagagga atgaaatcga
     2101 aaaaatcaag ctattgtttt taattggtgg ataatttcca gaggctgatt tgagttaaaa
     2161 acagtattcc caaccaccaa cacatccact ccttgagcaa aaagttttgg agcattttcc
     2221 aaatttatgc caccatctac ttccagcaaa caagaaggat ttttttgctg gattattgac
     2281 tttaattcat tgattcgttg ataagtttct tcaatgaatt tttgacctcc ataaccagca
     2341 aaaacactca ttaacaacac catatctact ttctctataa aaggttccag cacttttacc
     2401 gggatatcag gattgatggt aattcctgct ttcattcctg ctgcgtgaat ttgttcaata
     2461 atttcagcag ggtgttctgc tgcttcataa tgaaaagtaa gtatcccagc tcctgcttct
     2521 gcaaaacgtt gcacatattt ttcaggatga ataatcataa tgtggacatc cagtggtttt
     2581 ttacaatgtt tttttacagc tttaattaca ggaaaaccaa aagaaatgtt gggtacaaat
     2641 acgccatcca tcacatccag atgaatccaa tccgcttcgc tctggttaat aagttcgcat
     2701 attgcttgca gattactaaa atctgctgtt aatattgaag gagaaataag atttcccatt
     2761 gttgaaaaca attaaaataa attgtaattt tacaaaaata ataaaaggtt cttatccaat
     2821 caaagaacct tttatttatt ttgaaatttt aaaatagaat ttagagaaaa tttctgtatg
     2881 atactgtttt tctttgaatt aataactttt ttaaatatct aatttaattt acaagtatct
     2941 taaatctttg attatcaatt tttattttta actctttctg aaatgcgaaa ctttagtaaa
     3001 ataataatac tctcttgttt ataattaaac gttaagctaa caaattataa ttaaccccct
     3061 attgttaaga cccaattaga agtgttgaac aaatatatta tcttttttgt aaatttgtca
     3121 ttaagattat tgaaaaattc tttaatcggt cagcatccat ttaggtctct gttcctttga
     3181 aaaagtttgt tttatggttt ctaataaaaa tatctcccct tataacagaa tatgactcaa
     3241 aattacttga aagcgctttc aaaaaacaaa ataaaactaa tttcccgtct tcaacaaaaa
     3301 aagtggagag atgaattagg ttgtttcctt gccgaaggga caaaattagt tacagattta
     3361 agtacttctt tcaaaggcat gttgttggta gctactcctg attggttaaa aaatcactta
     3421 cctattcaag cagaagaaac tcttatagta acaagcgagg aatttaaaaa aatttctaca
     3481 caaaaaacac ctcaaggagt attggctatt tttaaaaaaa aagaaactaa atggtcgttc
     3541 cttgaaattt cttctcaatt atgctttgct ttggaagata tacaagatcc aggcaattta
     3601 ggtactatta tacgcgtagc tgattggttt ggcatttcaa atatattctg ctctgaacat
     3661 tctgctgatg tttatgggac aaaagttgtt caggctacga tgggagcatt agcacgagtt
     3721 caggttcata tcgttcattt acctgatttc ctcattgaaa gtaagaaata tcaaatacct
     3781 ctgtatggta ctttccttaa aggtgaaaat atttttacaa cttcacttac cccggaaggg
     3841 atcattgtgt tgggcaatga aggtaatggt atttcgtctg tactggaaaa tattattcca
     3901 actcgtctga ctattccatc ttatccatca gaaaatttca cttctgaatc attgaatgtg
     3961 agtgtggctg caggaattgt tgcagccgaa ttcagaaaaa gacttatgat tcacaaagga
     4021 atttgaatga aatttgaagg ataaaaaatt ataatccttc aaaattatcc attggaaatt
     4081 cttctccatt attattttca gtggattttt tcttttgaga tgttggcttc atgtttccaa
     4141 aattatagga aatggttaat cctaccattc gtccatgaaa gtaagattct gtttgttgat
     4201 aaaaatcttc tccccaagta gtagaacgca tttttcgcga attcaataaa tcacgcacca
     4261 ttaagctcag gtttaattta cggtccataa atgtttttct taatcccaga tcaatcgaat
     4321 atctttcttt ccttttccct tggcttataa tggttggcga agaataattt ccagaaattt
     4381 gagcggaaaa agttttagct aataaaacat tggcaatcac tcttgcattc catgaaaaat
     4441 tttcctgtgc aggaatatga caaattgtat tatatgggct tatataggta gcagagtcca
     4501 atttattaaa atataaattt agagaagaag ttaaattcaa aacatttgaa aaattatctt
     4561 tcacaactac ttccaaacct gaattttgag tttttgtaat atttagatag gtgttttcca
     4621 tgttgtttcc atgcatgtat cgcacatttt gtatcacatt gtttcttgaa cgataataag
     4681 cagaaaatga caatgaatga taataccaat ttttcaaata attcaattca taagaagaag
     4741 aaaactgggg tttaagctca ggattgccaa aggaaatatt agtggaatct gtataatcgc
     4801 ggaagggatt aatcattctt cctcgcggtc tgtccactct acgggtataa ttcaactgga
     4861 tttccccatt ttgggggagc gtataagaaa tatatatact tgggaaaagc tgaaaataag
     4921 tttgtggatc caattgagct attgaatcat aagtttcatt agtaatttgt ttccacatgt
     4981 actctgctct caccccccct tgaaggctta aacgattaaa acggcctcca taagttacat
     5041 atgcagcatg aatttgttcg tcataatcaa aattattata ataagaagga atgttagctc
     5101 cctgctgtaa attatctact cctgagacca aactgtttct actttgaatt ctgctttgcc
     5161 agcctgcttc caatttatta ttttctgtaa atttattagt ataatccact ttaaaattcc
     5221 aattattatt ctggttatcg gaattttgag taatatcact ggtggcttgt tctaaactat
     5281 cttcccgttg aataaattgc ttttctgaat ttctatcatg attagaataa gtaaggcttg
     5341 ctattaaatt agttcctttt ttatcaaaat catgttgata atccagtgtt aaattataac
     5401 tcggatgagt acctttttca aaagtatttc gactataatc acgaatggta tactgataaa
     5461 taggacttgt caataaataa tccaaaaaat tggaagaatt ttctttttga tacatactaa
     5521 aacccgaaat tccaatcgaa tttttatcag ttaaatgaaa atcagcgccc gctcgcaaaa
     5581 acaaaccatc aaaggaatga ttcattgcat tattttgcga taacaaagtg gtatccatcg
     5641 ctaaataact ataacgacga ttccaacctc cgcctttcat gttcattgag cgataaccaa
     5701 tgttggcata agcatcaact ttgctactag aataattaaa atttccacct aaagttttgc
     5761 ctagtttagc attttcagga tacataagtc ctgctgaaag acttccataa tatccagctt
     5821 tacgattttt tttcaatacc agattaataa ttcctgctgt tccttcagga tcgaatttgg
     5881 cagaaggatt ggtaattatt tcgatagatt caatactttc agcaggcatt tgttgaagta
     5941 cttgtgctcg attttcagtc gtcaaaccag caggtttacc attaatccat acttctacat
     6001 ttgaattgtt tcgtaacgaa atattacctt cattatccac atcaacagaa ggaatatttt
     6061 gcaaaacttc agtagccgaa cctcctgcag aggcaatatt ttgatccaca ctaaatactt
     6121 ttttatcaat atcaaatttc atttgaaaac cttgagctac tacttcaacc tctgaaagaa
     6181 attttgtttc ttcttttaaa gaaataattc ctaaatcaat ggagctttta tctatattta
     6241 tttgttt
//
LOCUS       DAOTKS010000043         6217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_11690_length_6217_cov_2.942227, whole genome shotgun sequence.
ACCESSION   DAOTKS010000043 DAOTKS010000000
VERSION     DAOTKS010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6217
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11690_length_6217_cov_2.942227"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..343)
                     /locus_tag="P5236_01505"
                     /pseudo
     CDS             complement(1..343)
                     /locus_tag="P5236_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561488.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase family protein"
     gene            complement(365..2140)
                     /locus_tag="P5236_01510"
     CDS             complement(365..2140)
                     /locus_tag="P5236_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HRU63071.1"
                     /translation="MGKRYLNRPSNFNKTPYIEVKNDSYKITKGWDNVVDELNKVINK
                     IDKKRKIIVIETYQGVIHEEVMINLQKGLQWSKFINAEELMFPEKKIKELVFPDVTND
                     RLFGYLTRLHIEDFYNTKKVKKFQAKLKRIKEGTIIVYGTGAAYVCPESDLLIYADMA
                     RWEIELRMRKHLVDNIGVKNRNVEDSALLYKQGFFVDWRVCDRFKKKLFKRWDFLLDT
                     NKVNNPKLITAKAIFEGLEIATRRPFSVVPFFDPGPWGGQWMKEVCDLDKDTINYAWS
                     FNGVPEENSLLLKFGDDIIEIPSIDLVFLHPRELLGEAVHGRFGDEFPIRFDFLDTMD
                     GGNLSLQVHPLTEYIQEKFGMHYTQDESYYLVEADTDAQVFLGLKEGINSTLMITELE
                     EAQAGGKPFDAEKHVQTWPVKKHDHLLIPAGTVHCSGKNCMVLEISSTPYIFTFKLWD
                     WGRLGLDGRPRPINIEHGKNVIQWDRTTNWTRKNLVDQVEKIAEGDGWIEERTGLHER
                     EFIETRRHWFTKKTEHDTQGKVNMICLVEGDEIIVESLSNTFEPFIVHYAEVFIVPAI
                     VGKYTISPHGSSIGSTCVTVKAYVK"
     gene            complement(2214..3167)
                     /locus_tag="P5236_01515"
     CDS             complement(2214..3167)
                     /locus_tag="P5236_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRU63072.1"
                     /translation="MYTIAIDLGGTNIKTGLLDRKNLIASTSEKATSGKGLKNNLPLI
                     EKMIDYLLENQKLSTKDLLGIGFSFAGMVDSAHNRVISTNKKYDDAPYLDLPRWAKEK
                     WNLPLYMENDARMALLGEWQHGAGKGYDNIVIVTLGTGIGSAVLIEGKLLRGKHFQAG
                     NLGGHFTINYQGTVCTCGNIGCMESEASTWRLPELIRNYPSYHSSSLANEKILDFEAL
                     FRNVAKNDSLAVEVLDHCFSVWSAGIITMIHAYDPEIVILSGGIMKSASIILPELQER
                     VNQYAWTPWGKVKLIKAENIETSALYGGDYLVRLHQQEQKN"
     gene            complement(3148..4326)
                     /locus_tag="P5236_01520"
     CDS             complement(3148..4326)
                     /locus_tag="P5236_01520"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRU63073.1"
                     /translation="MDSKKVSYAFVAPVLLSFFVMSACDLVGIGVDNVKADLNLNNTL
                     SQLIPLAVFAWFFLLSVPIGLLQDRMGKRNMLTIGILLTAIGLFLPYFTYSFLMVMIG
                     FGLLGIGNTIIQVSANPLLIDVVPEERASSFLSFSQFVKSVGSMVAPYLTTFFIVRFG
                     NWKLTLLTFGVISLLSASWLISIKIPESLLNEKKATFRSCFKLLEIKYVVFMVLAIFF
                     VVGIDVGINATSGQFLLIKIHTSVETAQEGRSLYFFGKMLGTFIGTFLLIRFSSSKFL
                     LASSIATFLTALLFIFSFSPLFAQIIMFLIGLSASNIFPLIFSLTVKEYPERANEISG
                     LMIMAVSGGAIIPFIVGVIADIFNIRTGMYVFVFCSLYLIFVSLYLFNKNKKNVYNSN
                     "
     gene            complement(4437..6197)
                     /locus_tag="P5236_01525"
                     /pseudo
     CDS             complement(4437..6197)
                     /locus_tag="P5236_01525"
                     /inference="COORDINATES: protein motif:HMM:NF019583.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
BASE COUNT         2051 a         1213 c          918 g         2035 t
ORIGIN      
        1 caaatttcat aaacttcagg agttgaccag ccactcaaag gtgatatatt ttatggatgt
       61 gagcttgact acgaacaggt tctttcccta atttacctgc tgcataagta accactccaa
      121 aaagtaagtg catatttttc aataattcaa gatgttcttt tttacccaca tccatcgcaa
      181 tagcatatac aatttcaggt ccttcacagt ttggatcaag gagacttttg cgaatatcat
      241 ttaaatgacg attctcaaca gtagggccaa agatatcaac tccatattta aaacctaatg
      301 gattgacaat agtttcaata tcaattcctg gattaaattt catattttta tttttaataa
      361 tttattattt aacatatgct tttactgtta cacaagtact tccaatggaa cttccatgag
      421 gagaaatagt atatttccct actatggcag gaacaataaa aacttcagca taatgaacta
      481 taaagggctc aaaagtattg gaaagacttt ctactattat ttcatcacct tctactaaac
      541 aaatcatatt aacttttccc tgagtatcat gttctgtctt ttttgtaaac caatgacggc
      601 gggtctcaat aaattcacgt tcatgcaagc cagtacgctc ttctatccaa ccatctccct
      661 ccgcaatctt ttctacttga tcaactaaat tttttcttgt ccaattagta gttctatccc
      721 attgtataac attttttcca tgctcaatat taatgggtct cggtcttcca tctaaaccaa
      781 gacgtcccca atcccataat ttaaaagtaa aaatataagg agtagaactg atctcgagaa
      841 ccatacaatt ttttcctgaa caatggactg ttccagccgg gataagaaga tgatcgtgtt
      901 ttttcacagg ccaagtttgt acatgctttt cagcatcaaa aggtttacca ccagcttgag
      961 cttcttccaa ttcagttatc atcagtgttg aatttattcc ttcctttaaa ccgagaaaaa
     1021 cttgtgcatc ggtatcagct tcgactaaat agtaactttc atcttgggta taatgcatcc
     1081 cgaatttttc ttgtatgtac tctgtaagag gatgaacttg taaactaaga ttaccaccat
     1141 ccattgtatc aagaaagtcg aaccgaatag gaaattcatc tccaaaacgt ccatgaacag
     1201 cttcacctaa taattcacga ggatgaagaa aaactagatc aattgatgga atttcaataa
     1261 tatcatcacc aaattttaat aataggctat tctcttccgg aacaccatta aatgaccaag
     1321 cataattgat agtatcttta tcaagatcgc atacttcttt catccattga ccaccccatg
     1381 ggcctggatc aaaaaatggg actacactaa aaggtcttct tgtagctatt tctaaacctt
     1441 caaaaattgc cttagctgtt attaattttg gattatttac tttattggta tcaagcaaaa
     1501 aatcccaccg tttaaaaagt ttctttttaa aacgatcaca aacccgccaa tctacaaaaa
     1561 aaccttgttt ataaagtaat gccgaatcct ctacattacg attttttact cctatgttgt
     1621 caaccaaatg tttacgcatt cttaactcaa tttcccaacg agccatgtct gcatatatca
     1681 acaggtctga ttcaggacaa acataagctg cacccgttcc gtaaacaata atagttcctt
     1741 ctttaattct ttttaatttg gcttggaatt tttttacttt tttggtatta taaaaatctt
     1801 ctatatgaag tcgagttaaa tatccaaaaa gtctgtcatt ggttacatca ggaaaaacta
     1861 attcttttat tttcttttca ggaaacatta attcttctgc atttataaac ttgctccatt
     1921 gaagtccttt ttgcaagttt atcattacct cctcgtggat aacgccctga tatgtttcaa
     1981 taacaatgat ttttcttttc ttatcaattt tattgatgac cttattgagc tcatctacca
     2041 cattatccca accttttgtt attttataag aatcattttt tacttcgata tatggagttt
     2101 tattaaaatt tgatggccta tttaaatatc tttttcccat gaactaaaaa tgaattatcg
     2161 ataaatatta gaaaaattaa gaatcctaga aggagatatc ttcatcaatt tgtttaattt
     2221 ttctgttctt gctgatgaag acggacaaga taatctcctc catatagtgc tgatgtttcg
     2281 atgttttcag ctttaattaa ttttaccttc ccccacggcg tccaggcata ttgatttact
     2341 cgttcctgca attcaggcaa aataatagaa gcacttttca tgattcctcc actaagaatt
     2401 acaatttcag ggtcataagc atgaatcatc gtgattattc ctgctgacca taccgagaaa
     2461 caatgatcaa gaacttcaac agctaatgaa tcattttttg caacatttcg aaataatgct
     2521 tcaaaatcca gtattttctc atttgcaagt gagctggaat gataggaagg ataatttcta
     2581 attaattcgg gtaatcgcca ggtagaagct tctgattcca tacaaccaat gttaccacat
     2641 gtgcagaccg ttccctgata atttatcgta aaatgcccgc caagatttcc tgcttgaaaa
     2701 tgtttacctc gtagtaattt cccttcaata agaactgccg aaccaattcc tgtacccagc
     2761 gtaactatta cgatgttatc gtatcctttc cctgctccat gttgccattc gcccagtaat
     2821 gccatacgtg catcattttc catataaaga ggtaaattcc atttttcttt tgcccatcga
     2881 ggtaaatcca aataaggtgc atcatcgtat tttttattag tggatattac ccgattatgt
     2941 gcagaatcaa ccataccagc aaaggaaaaa ccgataccta agaggtcttt ggtactcaat
     3001 ttttgatttt ctaacaaata gtcaatcatc ttttcaatta aaggaagatt gttttttaag
     3061 cctttgccag aagtagcttt ttcggaagta gaagctatca gatttttcct atcaagtaaa
     3121 ccagttttga tgttagtacc acctaaatca attgctattg tatacatttt ttttattttt
     3181 attaaacagg taaagagata caaaaattaa atagagtgaa caaaaaacaa agacgtacat
     3241 ccctgtccta atattaaaaa tatctgcaat tactcctact atgaaaggaa taatagcacc
     3301 gccagaaact gccatgatca ttagtcccga tatctcatta gctctttcag gatattcttt
     3361 cacagtcaaa gaaaaaatca atgggaaaat atttgaagct gacaagccaa ttaagaacat
     3421 aataatttgt gcgaacaaag gagaaaaaga aaagataaag agcaaagctg tcaagaaagt
     3481 tgcaattgag gaagcgagca aaaatttcga agatgaaaat cgtatcaata gaaaggtccc
     3541 aataaaagtt cctaacattt tgccaaaaaa ataaagactc ctaccttctt gggcagtttc
     3601 cacactcgta tggattttta taagtaaaaa ttgtcctgag gtagcattaa ttcctacatc
     3661 tattccgacg acaaaaaata tggccagtac cataaaaaca acatatttga tttccaataa
     3721 tttaaagcag gaacgaaaag ttgctttttt ttcgttaagc aatgattctg gaattttgat
     3781 cgaaataagc caacttgctg acagaagtga aatcacacca aatgtgagta aagtaagttt
     3841 ccaattgcca aatcgaacga taaaaaaggt tgttaaataa ggtgcaacca tggagcctac
     3901 tgatttgaca aattgagaaa aactcaaaaa gctggaagcc ctttcctcag gaaccacatc
     3961 aatgagcaat gggttggcag aaacttgtat gattgtatta ccaataccca aaagaccaaa
     4021 accaatcatt accattaaga aagaataagt aaaatatggc agaaaaagtc ctatcgcagt
     4081 aagtaaaatg cctattgtaa gcatattacg tttccccatt cgatcctgaa gtaagcctat
     4141 aggaacagaa agtaaaaaaa accatgcaaa aactgccaaa ggtataagct gcgaaagggt
     4201 attattcaaa ttcaaatctg cttttacatt atcaacgcca atacctacta aatcacaagc
     4261 gctcataacg aaaaatgata aaagtactgg tgcaacgaaa gcgtaagaaa ctttttttga
     4321 atccattctt aagctctttt tttattttta attaaaataa taattgtaaa tatcaacgca
     4381 tgttcattga taaaggaaaa ttttttcgag aatagtttag aaattcattt tctcatttat
     4441 ttagaaattc cccaactttt atttggggtg tctcctaata tcaattttag tactcctcct
     4501 tgagcaaaat catgatgagt gaaccaaaag gtatttaatg gtttttcgtt gagaaaagct
     4561 ttttgtatat acctattttc tatagaatta ttttgagtct ctattacaaa agtttttcct
     4621 gggtaatatg aaggatttaa ttcaattgta attttatcga aaataggagt agttatatca
     4681 tacactggtt ctacatctac acatcccttc aagctaaata aaccaattgc cattaatgca
     4741 cttaatcctc ccatttgacc ttgatcttcg tcatgacctc catagccaag atcaggagtc
     4801 gtaccactaa aagtttgttc tcgtactttc ctaacccaat attgagataa ccagggtttt
     4861 ccagcataat taaagacatg ggcattagca caacttggtt gattggcata attgatataa
     4921 ccttcagaat atgaaaacac aaaatcattt gcttctgaca tttcaaaagc ataattaagt
     4981 ttcttacaga gggtatcatt tcctcccata agctttgcca atcctggaat atcatgggaa
     5041 acagcgcaag ttgcttgcca cgaattggcc tctacccatc caacccctga aagaggatct
     5101 ttatgaataa aattaccttt tcgatctttg ggaaaaagta atttttcctg aggatcaaac
     5161 aattttttcc aaccagaaga acgctttaaa aaaaactgat aatcttttta tcttcccatc
     5221 ttttgagcca tttgagcaac tgcataatct tcgaaagcgg cttctacagt aattccagca
     5281 ttatcaggcc aatagccatg acgggtataa aattcgactt cttccttgtt tcctaacatg
     5341 cctccaggca aatgattctg tcgtataatt tggtaagtta agaaagcatc ttttttagtt
     5401 aacagatttc tcatataagc actaacaatc atgttcgtgg tcggacaatt agtcataata
     5461 taggaatagc cacctccaca tggacctctt ggcaaaagat aaccattttc agcatattga
     5521 atcattgatg cgctcatgtc atcaagtatt tcaggccatg ctaagcccca taggatattc
     5581 aaattccatt gagtgcccca aaaagcatcg ctattataca tatggtattt aggattgcca
     5641 tttgcatcta aaggtaattg gcgaatttta aaaatagcat ctgtgaagtt tccttctctt
     5701 cttcctgaag ttcgatcagg ataataaccg tttatatcat cgattttttg acgtcctaat
     5761 aaaacatgcc ataaatctgt ataaaattta atctgttgtt ctggaacacc accttctacc
     5821 ttgatttttc caagcatttc attccaggta agttgagagt tgtgacgcac ctcatcaaaa
     5881 ttccatgctg ggcattctat ttcgaggttt tttcgagcat tttcaatact ggtgtatgaa
     5941 acagcaattt tcatttgaag tttatcgccc ggatgcatag aataaatagc actcacacct
     6001 gtactgtctc ctgaaatttc agaaatatta tgatatctct ttgtgccttt ccaaccatca
     6061 agttgtttaa aaggttcatc aaattgtgcg ataaaaaata cttcaacatt ttttggaccg
     6121 ccccaaaacc tatcggtgga attaaagtaa ccctctattt ctcgatcatt tactttcttt
     6181 actgacgctg ctttcatagt tgaattacca agatatc
//
LOCUS       DAOTKS010000044         6215 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_11696_length_6215_cov_5.075000, whole genome shotgun sequence.
ACCESSION   DAOTKS010000044 DAOTKS010000000
VERSION     DAOTKS010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11696_length_6215_cov_5.075000"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..106)
                     /locus_tag="P5236_01530"
     CDS             complement(<1..106)
                     /locus_tag="P5236_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HRU63074.1"
                     /translation="MNIIKFSSRFPDEKSCIEHFKAQRDACGVICPKCG"
     gene            1194..1571
                     /locus_tag="P5236_01535"
     CDS             1194..1571
                     /locus_tag="P5236_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRU63075.1"
                     /translation="MKKIINTPNAPAAIGPYNQAIEINNMLFISGQIPILPKEGKIVS
                     LTVSEQTEQIFSNIEAILKFAGYSFENVIKTTVFLTDMNHFAAMNEVYKKYFTNNCPA
                     RSTVAVKALPHGALVEIETIAIK"
     gene            complement(1577..2533)
                     /gene="fmt"
                     /locus_tag="P5236_01540"
     CDS             complement(1577..2533)
                     /gene="fmt"
                     /locus_tag="P5236_01540"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRU63076.1"
                     /translation="MGKTKEELRIVFMGTPEFAVESLKTLVENGYNVVGVVTMPDKPA
                     GRGYKVQFSPIKQYALTKNLNLLQPEKLKDETFLENLRALKADLQIVVAFRMLPEVVW
                     NMPPLGTFNLHASLLPQYRGAAPINWAIINGEKETGVTTFFLTHEIDTGKIILQEKIP
                     ILPSDNAGTLHDKLMGLGAELVKKTVDMIISDKIQTIEQSQLVKNNIELKSAPKIFKE
                     TCEIDISWNVERAHNFVRGLSPYPGAWLGINFPNSPERIILKIFRTEPEFSSHQLPLG
                     KVVTDGKKFAKIALKDGFLVLKEVQAPNKKRMEIGEFLRGLQ"
     gene            2771..3445
                     /locus_tag="P5236_01545"
     CDS             2771..3445
                     /locus_tag="P5236_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRU63077.1"
                     /translation="MSKKEKKKDEFKNVEGALTASEIFIEKYQKQILYVVGGIVLVVL
                     LLLAARNFYIQPREKAAENEMYKAQYYFSVDSFQVALEGKEPEFIGFKQIVSEYGFTN
                     SGKLAAAYAGICYYKLGDYKNAIKYLTQFDKKDTYFTTAVLGLIGDSYVELGEKDKAL
                     GYFEKAIATKNNVLTPVYLKKAGLLCESMNQPQKAEKYYMEIKENYPKSMEATDIDKY
                     LARVQK"
     gene            3493..4029
                     /gene="ribH"
                     /locus_tag="P5236_01550"
     CDS             3493..4029
                     /gene="ribH"
                     /locus_tag="P5236_01550"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRU63078.1"
                     /translation="MSTQYKNLSSYNEDEMPDKKKVFTQQYAIVVADWNPQVTQSLLQ
                     GTYDALIENGANPDLITIIHVPGTFELIFAAKQFMDEYSEIDNIKVHKYAAIIALGCV
                     IQGETPHFAYVCQGVTYGIATLNANSNGCPVIFGILTTQNLQQALDRAGGLHGNKGFE
                     AGITAIKMANIDWHTSIF"
     gene            4484..5164
                     /locus_tag="P5236_01555"
     CDS             4484..5164
                     /locus_tag="P5236_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HRU63079.1"
                     /translation="MKKQVSLSFMFCSLLFTVCLIVANIVEQKLISIGPIEATAGLLI
                     FPVSYIINDLIAEVWGYKKARLIIWGGFAMNFLAVLIFKLSIIVPGSENFSHQEAFAL
                     VLNNTQRITFASFLAFLVGSFLNAYVMSKMKILQHGRNFSLRAVASTLVGESGDSLVF
                     FSIAFGGIISLHDLLLLIVTQTTMKTLYEILVLPLTNVIVKWIKKKENLDTYDYQISY
                     NPLKVREI"
     gene            complement(5204..>6215)
                     /locus_tag="P5236_01560"
     CDS             complement(5204..>6215)
                     /locus_tag="P5236_01560"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRU63080.1"
                     /translation="SPENQNAIRIMTIHKAKGLAFKVVILPFCDWNMDTRQRNILWCE
                     PQISPFNEFPLLPIEYTSKLENSIFTVDYFNEQLHLYIDNLNIAYVAFTRAKNELIGF
                     CKMPKKNSKVTSLSTLLMISFENVAENSELNAHFSSETNTFEWGEPTFANRELENENF
                     TSEKINYYPSVSSKDRLKIRSQNFIQFLDEQHLTDTRLNYGIVMHEVLRQMITRNDQE
                     KAIQKMIMMGAITVEESKIVEQELESFWNLPETKEWFGANVKIMNEVTILTPSGEQFR
                     PDRVVIHGNEAMVVDYKFGEKESENHLRQVKRYMNLISQMGYAVKGYVCYVSLGKVME
                     VN"
BASE COUNT         2151 a          937 c          931 g         2196 t
ORIGIN      
        1 agccacattt tggacaaatt actccgcaag cgtctctttg agccttaaaa tgctcgatac
       61 aggacttttc atcggggaat cgtgaactga atttaatgat attcataaga attatagttg
      121 ttgatttgaa gtgtaaagat aatgaatatc agtgaattat acaaatattt taataactat
      181 tttttgaaaa atatactgta atttatccct tttaaagtag gatactttgc ggataatcat
      241 tattttaatt attataaaaa tataaaaaaa tatatattat tatattccag taccttattt
      301 taaattttaa aaatgtgtaa atttcttttt tagataaaaa ctgaaccata ttcccgaaaa
      361 ggaattacac agacaaaata atgttgaagc catcattctt caattagtta ctattattct
      421 accaataaga cacgttataa aagatttact actgattacg gttgtttttt tgctcattat
      481 aaaaactttg tctaaatgct aaaatatatt tgtaaaccag tcaaaaaacg ccttttaaaa
      541 aaaaaatact atacttcgaa aattttttca agagactata aataaatatt ttaattgata
      601 tataataaag attaagataa taatgagttg ttgaaaaagt ggataaattt tttttaaaaa
      661 gtaaaatatt ttttttcaac tctttatatt ggtttttcaa cagaaaagaa aagtgtattt
      721 attttatttt caaaaagttt tatttgttat taacaatcta tttaaaaact taaagttaaa
      781 aatttctttt ctttgcaaaa gaaaaaatta gttagtaaat aattaaaaaa agaaataata
      841 attttttttt atttcataat aattgttata taaaaatagt ttttatagtt ggtacttgaa
      901 tattttgttt tttctcaaac ctttttgaaa aattttcaac caaaaaacac tatatattaa
      961 cagaattgag gtataaattt aaaaatgaat agttatttat attattttaa aaaattattt
     1021 gtaaaaccta tttcaaaaag tcaattttaa tgattctgta atttataagt tattgattta
     1081 gttatttttg caactattgt tgaagctaaa ctgtgaaaat atgatgagaa aatttatatt
     1141 aaaaaaatta agtacgattt atttaaagtg tttattctaa attttttatt attatgaaaa
     1201 aaattatcaa tactcccaac gctccggcag ctattggacc ttataatcag gctatagaaa
     1261 taaataatat gctttttatt tcaggacaaa ttcctatttt gccaaaggaa ggaaaaattg
     1321 tttcattaac tgtttcggaa caaacagaac agattttttc aaatattgaa gctattttaa
     1381 agtttgctgg ttattctttt gaaaatgtaa tcaaaacaac cgtttttcta acggacatga
     1441 accattttgc ggctatgaat gaagtatata aaaagtattt caccaataat tgtcctgctc
     1501 gttcaacggt tgccgtaaaa gcacttccgc acggagcttt ggtggaaatt gaaacaatag
     1561 ctataaaatg aaaaatttac tgcaaacctc gtagaaattc gccaatttcc attcgctttt
     1621 tgttgggggc ttgcacttct ttcaaaacta aaaaaccatc ctttaaggct attttggcaa
     1681 attttttccc gtcggttact acttttccta atggtaattg atgagaagaa aattctggtt
     1741 ctgttcgaaa aatctttaaa attattcttt caggagaatt tggaaaattt atccccaacc
     1801 aagctcccgg ataaggagaa agccctcgta caaagttatg agcacgttca acgttccagg
     1861 aaatatcaat ttcacaagtt tctttgaaga tttttggagc cgatttcaat tctatgttat
     1921 ttttcactaa ttgagattgt tctattgttt gaattttatc tgaaataatc atatctaccg
     1981 tttttttaac caattctgca cccaatccca ttaatttatc gtgcaaagta ccagcattat
     2041 cagaaggtaa aatgggaatt ttttcctgaa gaataatttt accggtatca atttcgtggg
     2101 ttagaaagaa agttgttaca ccagtttctt tttcaccatt gataattgcc cagttaatag
     2161 gagctgcacc tctatattgt ggaagtaagg aagcatgaag attgaaagtt cccaatgggg
     2221 gcatgttcca aactacttca ggtaacatgc gaaaggctac cacaatttgt aaatctgctt
     2281 tcagtgctcg tagattttct aaaaaagttt cgtctttcaa tttttcgggc tgaagcaagt
     2341 tcaagttctt cgttaaagca tattgtttta ttggtgagaa ctgaacttta tagcctctgc
     2401 cagcaggctt atcaggcata gtaaccacgc caaccacatt ataaccgttt tcaactaatg
     2461 tttttaaact ttcaacagcg aattctggtg ttcccataaa aacaattcgt aattcttctt
     2521 ttgtttttcc catattttct aatctttttt aagatagtca ttcttttgcg acaaaaataa
     2581 taaaaaaatc taccaactct tatgaataaa gcataaaaca gttttttcta caatttattt
     2641 attttatgcg aataattgga agctttaaaa acttaaccta aaatttcatt atctaaaaga
     2701 aaaatcttaa ttttgttgct catttattta aacaatcttc aatttaataa actaaaagaa
     2761 gaccattttt atgtcaaaaa aagaaaaaaa gaaagacgaa tttaaaaatg tggaaggagc
     2821 tttaacggct tcagaaattt ttatagaaaa gtatcagaaa caaatattat atgtagttgg
     2881 aggaattgtt ctcgttgttt tgctattatt ggcagctcga aatttttata ttcaaccacg
     2941 agaaaaagcg gctgaaaacg aaatgtataa ggctcaatat tattttagtg ttgattcatt
     3001 tcaagtagca ttagaaggaa aagagccaga atttattgga tttaagcaaa tcgtttcaga
     3061 atacggcttt acaaattcag gtaaactcgc tgctgcttat gcaggtattt gttattacaa
     3121 attgggtgat tacaaaaacg ctattaaata tcttactcaa tttgacaaaa aagacactta
     3181 ttttacaaca gcagtacttg gcttaattgg agatagttat gtagaattag gagaaaaaga
     3241 taaagcttta ggatactttg agaaagctat tgcaacaaaa aacaatgttt taactcctgt
     3301 atatttaaaa aaagcaggtt tattatgtga atcgatgaac caacctcaaa aagcagaaaa
     3361 atactatatg gaaataaaag aaaattatcc taaaagtatg gaggctacag acattgacaa
     3421 atatcttgct cgcgttcaaa agtaaacaaa tttataatct tcattaaaaa aattatttta
     3481 aaaaaaatac aaatgtcaac gcaatataaa aatttatcca gttataatga agatgaaatg
     3541 cctgataaaa aaaaggtgtt tacacaacaa tatgctattg tagtagccga ttggaatcct
     3601 caagtgacac aatcattact gcaaggtact tatgatgctt taattgaaaa cggagctaac
     3661 cccgatttaa ttactattat ccatgtaccc ggcacttttg agctcatttt tgcagccaaa
     3721 caatttatgg atgaatattc tgaaatagac aatataaaag ttcataaata tgctgctatt
     3781 attgccttag gttgtgtgat tcaaggagaa acacctcatt tcgcttatgt ttgtcaagga
     3841 gttacttatg gaattgccac attaaatgcg aattcaaatg gatgccctgt catctttgga
     3901 atacttacta ctcaaaattt acaacaagca cttgacagag caggcggctt acatggcaat
     3961 aaaggattcg aagccggaat tacagctatt aagatggcaa acattgattg gcacaccagt
     4021 attttctaag gattaaagct tatactgaac agataagtga aaatgaaatt aattatggaa
     4081 taaggagatt cttacttgct attggtaatt tatgattgct ttttgatgat tttgagtttt
     4141 atttgttaat tcttgggata aaaataattt tattggtatc caaacagcag caaagaaaat
     4201 ggtggagtag ctcatagttt catgttgtac aaataacgat gaataaaaac tatccaacaa
     4261 aagatggtga accctttttt atgtatgtta ccaatcaaaa gagctgcaac tctcaacctt
     4321 tttaacaaac caccatattt caactgatgt cgatagtggg agagagatcg gaaataaagt
     4381 aagaaaaagc tcctattttt ttcctactat tcgataaaaa acaattactt ttgcactatt
     4441 ttttgcaaaa cgtgtgcttg aaaaccacgt aaaaaacaag aaaatgaaaa aacaagtttc
     4501 tctctctttt atgttttgtt cgctactttt taccgtctgc ttgatagtag caaatatcgt
     4561 tgaacaaaaa cttatttcta tcggacctat tgaagccaca gcaggcttac ttattttccc
     4621 tgtttcttac attataaatg atttaattgc cgaagtatgg ggttataaaa aagctcgtct
     4681 cattatctgg ggtggttttg ccatgaattt tctggcagta ttgattttta aattgagtat
     4741 tatcgttcct ggctctgaaa atttttctca tcaagaagct tttgctttgg tgttgaacaa
     4801 tactcagcgt attacttttg caagttttct tgcttttctt gttggctctt ttttgaatgc
     4861 ctatgttatg agcaaaatga aaattcttca acatggacga aatttttctt tacgtgccgt
     4921 agcttccacc ctggttggcg aaagtggtga ttcattggtg tttttttcaa tcgctttcgg
     4981 tggcattatt tctcttcacg atttgttgct tttaattgtt acacaaacaa ccatgaaaac
     5041 cctttatgaa attctggttt tacctttaac aaatgtgatt gtaaaatgga taaagaaaaa
     5101 agaaaatctc gatacttatg attatcaaat ttcgtataat cctttaaaag taagagaaat
     5161 ttagctacta ttttggttta actccaaaga gttaattttt catctaattg acttccatta
     5221 ctttgcctaa actaacataa caaacatagc cttttactgc ataacccatt tgggaaatca
     5281 agttcatgta ccgtttcact tgccgtaaat gattctctga ttctttttct ccgaatttat
     5341 aatcaactac cattgcttcg tttccatgaa ttacgacacg gtcgggacga aattgttcgc
     5401 ctgatggagt aagaatagta acttcattca ttatttttac atttgctcca aaccattctt
     5461 ttgtttcagg caaattccag aaagattcaa gttcttgttc tacaatttta ctttcctcaa
     5521 ccgtaatagc tcccatcatt atcatttttt gtatcgcttt ttcttggtca tttctagtga
     5581 tcatctggcg taacacttca tgcatcacaa taccataatt caaacgagta tccgttaagt
     5641 gttgttcgtc taagaattga atgaaatttt gtgaacgaat tttcaaacgg tctttactgc
     5701 tcacggaagg atagtaattt attttttcgc ttgtaaaatt ctcattttca agctctctat
     5761 tagcaaaagt tggttcgccc cattcaaatg tattggtttc tgaagaaaaa tgagcattta
     5821 attcagaatt ttcggcaaca ttttcaaaac ttatcattag taaagttgat aaagaagtaa
     5881 ccttactatt ttttttaggc attttacaaa aaccgatcaa ttcgttttta gctcgtgtaa
     5941 aagctacata agcaatgttt agattatcaa tgtaaagatg caattgctca ttaaaataat
     6001 ctactgtaaa aatggaattt tccagttttg aagtatattc aattggcaat aaaggaaatt
     6061 cattaaaagg agaaatttgg ggctcgcacc ataaaatgtt ccgttgtcgt gtatccatgt
     6121 tccagtcgca aaaagggaga atcaccactt taaaagccag ccctttggct ttatgaatgg
     6181 tcatgatacg aattgcattc tgattttcag gcgat
//
LOCUS       DAOTKS010000045         6179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_11775_length_6179_cov_4.642227, whole genome shotgun sequence.
ACCESSION   DAOTKS010000045 DAOTKS010000000
VERSION     DAOTKS010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6179
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11775_length_6179_cov_4.642227"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..659
                     /locus_tag="P5236_01565"
     CDS             45..659
                     /locus_tag="P5236_01565"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HRU63081.1"
                     /translation="MNTYLFYSFCLLIGYLIGSILFARLITRLATGQNILELGNHNPG
                     AYNVFRNVGKGWGILVGILDAGKALFPMLIARYAFHLQDDIALGCLGIGALLGHGYPL
                     YYHFEGGRAASILMGMLIFFVPYEFLFSFLIGGAIVLLFIKKEYGIWGPTLIIAFSAI
                     SCLFFPHTVEVKIIIWIGALITLFFNRDAVQERTKKLSPVVKKK"
     gene            744..>1156
                     /gene="folK"
                     /locus_tag="P5236_01570"
     CDS             744..>1156
                     /gene="folK"
                     /locus_tag="P5236_01570"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRU63082.1"
                     /translation="MSLVYLSLGSNLGDKTLHLNSAIEKLNQLAGRVVRQSSFYVTKP
                     WGFESKNDFLNSVVLLETFLSPFELLGKIQQIEQMMGRVSKTINGDYSDRIIDIDILL
                     YDNLVMEHPSLTLPHPLMTSRDFVLIPLFEIAPNIT"
     assembly_gap    1157..1256
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1257..2173)
                     /locus_tag="P5236_01575"
     CDS             complement(<1257..2173)
                     /locus_tag="P5236_01575"
                     /inference="COORDINATES: protein motif:HMM:NF027441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4831 family protein"
                     /protein_id="HRU63083.1"
                     /translation="MKKLISFFSLISISLLLIAQNFSITENQPVIIYSLPMTQLCIEV
                     QMEKITQTPGIFYLYSERYLATKNVITEEKTTYSIKNITVYTKTIPDLQRTYSFVPTK
                     NSPLKNITLNQQGILCGINVACLPSPEGERPSVTLSSKEKKNFSHFLLPLGEEYLLAG
                     STAKLAEGAAKQIYDIRDSRLNILTCDVDHLPADGVSLNTLLRELNVREKELTELFIG
                     KTTSESLSQVLYLTPAKAMKNEILFRFSTLKGVVPSDDLSGIPYYITISPTTFPVATT
                     TKEKKEKTINKQQKLNTILPASTLVNIGDG"
     gene            complement(2242..4647)
                     /gene="topA"
                     /locus_tag="P5236_01580"
     CDS             complement(2242..4647)
                     /gene="topA"
                     /locus_tag="P5236_01580"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRU63084.1"
                     /translation="MPKNIVIVESPAKAKTIEKFLGKDYQVMSSYGHIRDLSEKNIGI
                     DFENNYQPLYEISPEKKKIVAELKKSIKDADMVWLASDEDREGEAIAWHLFEVLNLKE
                     EKTRRIVFHEITKDAILHAIEHPRPIDLHLVDAQQARRVLDRIVGFELSPLLWRKIRP
                     SLSAGRVQSVAVRLIVEREREINQFTPESFYKITGFFSGKDKNGSLVELKAELQQRFH
                     RKEEALAFLQKCKIEEFFIENIVTKPAKKSPAPPFTTSTLQQEAARKLGFSVSQTMRL
                     AQRLYESGKITYMRTDSVNLSELALNTSEVEIKKIAGPQYIKRRHFVTKTKGAQEAHE
                     AIRPTYMNVHEIEGTAQEKRLYDLIWKRTMASQMADAELEKTTITIAMKNSPYQFIAN
                     GEVILFDGFLKLYAESSDEENENGSTNILPTMKVGEGLSPSKIIAQQHFSQHPPRYTE
                     ASLVRKLEELGIGRPSTYAPIISTIQNRLYVEKKDRPAEKRDYNVLILNNNKIEDKIE
                     TEARGAEKSKLFPTDIGMVVNDFLMEYFPSILNYNFTANVEKEFDSIAAGKEKWTEVI
                     DKFYKKFHAAVEKTLTSKDRKTKERILGIDPETGKQVSVKFGKYGPFVQLGTGEDGEN
                     PTFASLPKNYSMELITLEEALELFKLPRNLGEYENQPLTVAIGRFGPYVRQGNSFYSI
                     PKNLDPLTITNEQAIEIINKKKNEEKNRTIATFGEKNEIQVLNGRYGPYIVYNKTNYK
                     IPKNVDPSTLTYEKCMELIKSQAEKNNKEKTTQKKGKSNTVTGKKLRGKKTQQRKRIE
                     NKK"
     gene            5505..>6179
                     /locus_tag="P5236_01585"
     CDS             5505..>6179
                     /locus_tag="P5236_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63085.1"
                     /translation="MKLYKKKIFFFLFFICLFYSCEDDNEYRVDSEFLPYLTRFEIEA
                     GKRGKNFNFQRDGIIIEFAKLKESTAGLTYKEHPVRIQIDKKYWEDISSSAGADIMKE
                     NLLFHELGHGILGRKHLNTVLENGDWKSIMCGGEKIDNRSWNINYRGFRRNYYIDELF
                     DESTTPPFLLTSIFNIDTLAYRLVIEKNFNDAQHAGWTIKNTTTYETSIDNRRLKFQS
                     KIDSLYF"
BASE COUNT         1888 a          939 c         1033 g         2219 t
ORIGIN      
        1 gaggttatga tatttctgta tatctaaaat aaaaaaaata tattatgaac acctatttgt
       61 tttattcgtt ttgtttgcta ataggttatt tgattggttc cattttattt gctcgtttga
      121 ttaccaggct agcaacggga caaaatattt tagagcttgg taatcataat ccaggtgctt
      181 ataatgtatt cagaaatgtt gggaaaggct ggggaattct cgtaggaatt ttggacgctg
      241 ggaaagcact ttttcccatg ctaattgcca gatatgcttt tcatcttcaa gatgatattg
      301 cattaggatg tttaggaatt ggagctttgt taggtcatgg ttatccttta tattatcatt
      361 ttgaaggtgg acgtgcagct tctatcttga tgggaatgct tatatttttt gttccttatg
      421 aatttttgtt ttctttctta attggcggag ccattgttct gctttttatt aagaaagaat
      481 atggaatttg gggaccaaca ctgattattg ctttcagtgc gatatcttgt ttgttctttc
      541 ctcatactgt tgaagtaaaa attattatct ggattggtgc attaattaca ttatttttta
      601 atcgtgatgc ggtccaggaa cgaacaaaaa agctgagtcc tgtcgttaag aaaaaataga
      661 aatcaacata aatttattat tactgtcaag aagtcagact tttcgaattt tttcaaaaat
      721 gtgactttat atttttgcaa attatgagtc ttgtttattt atcacttggg agtaatcttg
      781 gagacaaaac tttacattta aatagtgcga ttgaaaagct gaatcaattg gcaggaagag
      841 ttgtacgtca atcttcgttt tatgttacaa agccgtgggg atttgaatcg aaaaatgatt
      901 ttttaaatag tgtagtttta ttagaaactt ttctttcacc ttttgagctc ctgggcaaaa
      961 ttcaacaaat agaacaaatg atggggcgag tttctaaaac tataaatggc gattattcgg
     1021 ataggataat tgatatagat attttgcttt atgataatct tgtaatggaa catccttctt
     1081 tgactttgcc tcatccactt atgacaagcc gagattttgt tcttattccg ctatttgaaa
     1141 ttgcacctaa tataacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccat
     1261 ctccaatgtt taccaatgta gaagcaggta aaattgtatt taatttttgt tgtttattta
     1321 ttgttttctc ttttttttct ttggtcgttg tagctactgg gaaagtagta ggggaaatag
     1381 taatataata aggtatgcct gataaatcgt cagaaggaac aacacctttc aacgtagaga
     1441 aacgaaaaag aatttcattt ttcattgcct ttgcaggtgt taagtataaa acctggctca
     1501 atgattctga ggtagttttc ccaataaaaa gttctgttaa ttctttttct ctgacattta
     1561 attctctcaa caacgtattg agagaaactc catcggctgg caaatgatcc acgtcacaag
     1621 taagaatatt taatcggcta tcgcgaatat cgtagatttg tttggcagct ccttcagcta
     1681 attttgctgt agaacctgcc aacaaatatt cttctcccaa aggtaataaa aaatgagaga
     1741 agtttttctt ttctttggaa gacaaagtaa cagaaggtct ttcaccttca ggtgaaggca
     1801 gacatgcaac attaattcca cataaaatgc cttgttgatt gagggtaatg ttttttaaag
     1861 gagaattttt agtaggaaca aaagaataag ttctttgaag atcaggaata gttttggtat
     1921 agactgtaat attttttatg ctataagtag ttttttcttc cgtaatcaca tttttggttg
     1981 ctaaatatct ttcagaatag agataaaata tgcccggtgt ctgggtaatt ttttccattt
     2041 gcacttcgat gcacaattgg gtcattggta aagaataaat aatgacaggc tggttttccg
     2101 taattgaaaa attctgagca atcaacaata aacttatgga aatgaggcta aaaaatgata
     2161 ttaatttttt catgacaggt aaaatattaa aatatataga ttaatataaa aacgaaaaaa
     2221 caaggtttgt attttataat cttatttttt attttcaatt ctttttcttt gttgtgtttt
     2281 tttacctctt agctttttcc ctgttaccgt attgcttttt cctttctttt gggtagtttt
     2341 ttctttgtta tttttttctg cttgagattt aatcaattcc atacatttct catacgttaa
     2401 ggttgaggga tcaacatttt ttggaatttt gtaattcgtt ttattatata ctatataggg
     2461 tccgtatcgt ccatttaaga cctgaatttc attcttttct ccaaaagtag caatagttct
     2521 atttttttct tcattttttt tcttattaat gatttctatt gcctgctcat ttgtaatagt
     2581 aagtggatcc agatttttag gtatggaata aaaagaattt ccttgtcgaa catagggtcc
     2641 aaaacgccca atcgccacag ttaaaggttg attttcatat tctcccaggt tacgcggaag
     2701 tttaaataat tccagggctt cttcgagtgt aatgagttcc atcgaataat ttttcggcag
     2761 ggaagcaaaa gtaggatttt ccccatcttc tccagtacca agttgaacga agggtccata
     2821 tttcccaaat tttacggaaa cttgcttgcc tgtttcagga tcaattccca aaatacgctc
     2881 ttttgttttt cgatctttac tagttaatgt tttttcaacg gctgcatgga attttttata
     2941 aaatttgtca attacttcag tccatttttc ttttcctgca gcaatactat caaattcttt
     3001 ttcaacattt gcagtgaaat tataattcaa aatagaagga aaatattcca ttaaaaaatc
     3061 gtttaccacc attccaatat cggtaggaaa aagtttagat ttttcagcac cccttgcctc
     3121 ggtttctatt ttatcttcaa ttttgttatt attaagaatc agcacattat aatctctttt
     3181 ttcagcaggg cgatcctttt tctctacata caatcgattt tgaatggtag aaatgatagg
     3241 agcgtaagtg gaaggacgcc caattcctaa ttcttccagt tttcgcacta aactggcttc
     3301 ggtatatcga ggaggatgct gtgaaaaatg ttgctgagca ataattttgg aaggagataa
     3361 accttcacct actttcattg ttggaagtat attagtgctt ccattttcat tttcttcatc
     3421 actggattct gcatacaatt tcaaaaaacc atcaaataaa attacttctc cgttagctat
     3481 aaattgataa ggtgaatttt tcatggcaat ggtgatggtt gttttttcta attctgcatc
     3541 agccatttgt gaagccatgg ttcgtttcca aatcagatca tacaaacgtt tttcttgagc
     3601 tgttccttca atttcgtgta cattcatata agtaggacga atagcttcgt gggcttcctg
     3661 agctcctttg gttttggtta caaaatgtct tctttttata tattgaggtc cagcaatttt
     3721 tttgatttct acttcggaag tattcaaagc tagttcagat aaattgacag aatctgtacg
     3781 catgtatgta atttttccgg attcgtacaa gcgttgagcc aacctcatgg tttgcgagac
     3841 agaaaaacca agtttccttg ctgcttcttg ctgcaaggtt gaagtagtaa agggaggagc
     3901 tggcgatttt tttgctggct tggtaacgat attttcaatg aaaaattctt ctattttaca
     3961 cttttgtaga aaagccaaag cttcttcttt tctgtgaaaa cgttgctgta attctgcttt
     4021 taattcaacg agagaaccat ttttatcttt tccactgaaa aacccagtga ttttataaaa
     4081 agattcggga gtgaattgat tgatttccct ttcgcgttcc acaatgagac gtacagctac
     4141 ggattgaact ctgccggctg aaagggaagg acggatttta cgccataata gtggcgacaa
     4201 ttcaaatcct acaatcctgt ccagcacacg acgagcttgt tgagcatcaa ccagatgcaa
     4261 atctattggg cgaggatgtt caatggcatg aagaatggca tcttttgtaa tttcgtgaaa
     4321 aacaatccgt cgtgtttttt cttctttcag gtttaatact tcaaacaaat gccaggcaat
     4381 ggcttctcct tcacgatctt catcggaagc gagccaaacc atatcggcat cttttatgga
     4441 ctttttcagt tctgctacta tttttttctt ttcaggtgaa atttcgtaaa gtggttgata
     4501 attgttttca aaatcaattc cgatattttt ttcacttaaa tcgcgaatat gtccgtaact
     4561 agacattact tgatagtctt ttccaagaaa tttttcaatg gttttagctt tggctggaga
     4621 ttctacaatg acaatgtttt taggcataat gtgtaagtta tcaaatataa ttgcgtgcaa
     4681 agtaacaaaa attgccaggt atcagccaaa ttttcaactc attattttct caattttata
     4741 ataaacatat attttcatcc tttatccacc tgataataag atttataatc ttctactggt
     4801 actaaattgt tatcaaaatt attaaaatta aaatattcat ccaaaaataa aattgctata
     4861 ttatgaaaat tatacagaaa aagaaatatc atcttttact tgagtaaatt cattaaccga
     4921 ttgagacaaa gagaaaaact atgcttttag gaaatgtata atcttgtaaa tcagtatttt
     4981 atagatcaat acaaattgag gtatctctca agacaaggat ttgcaagaaa aaaactcaac
     5041 aatagctacc aaattaaaca atatttcaat agaaaactaa atttacaagc acaaaataca
     5101 aacacaatcc cgtagtttca actaaagatt taaatttttc aaacaaaatt tcttacttgc
     5161 ggattttagg tcataaaaat ttgttttttt aatctgggtg ttttcctcaa ccccactaac
     5221 ttgaagcttg cccggatttg taatgtgggt gtaccgaagg caaaaaaata ggatcacaaa
     5281 ttgcaaattt atgtcagcag aaaaaatttt aagttaatcg gcgtaaagta ttccgttatt
     5341 ctatggaaaa tccttgtgct cttatgcctc gcatattgcg aactccaaac cattgattgg
     5401 taattgaatt tctttatagc ataataaatt aagaattagt ttatagtttt aacaaattat
     5461 cgatgttttt atttatattt gtataaagat taggttagaa aaaaatgaaa ctatataaga
     5521 aaaaaatttt cttttttctt ttctttattt gcctttttta ttcctgtgaa gacgacaatg
     5581 aatatcgtgt ggacagtgaa tttttacctt acttaacacg atttgaaata gaagcaggta
     5641 aacgaggaaa gaattttaac ttccaaaggg atggaataat tattgaattt gccaaattga
     5701 aggaaagcac agcaggttta acttataagg aacatcctgt tcgcatacag attgataaaa
     5761 aatattggga agatatttct tcctcagcag gagcagatat aatgaaagaa aatttgcttt
     5821 ttcatgaatt gggacatggc atattaggtc gaaaacattt gaatactgtt cttgaaaatg
     5881 gcgattggaa atctattatg tgtggtggag agaaaataga caatcgttcc tggaatatca
     5941 attatagagg cttcaggaga aattattata ttgatgaatt gtttgatgaa agtactactc
     6001 caccttttct tttaacttct atttttaata ttgatacttt agcttatagg ttagtgattg
     6061 aaaaaaattt taatgatgcg caacacgctg gatggacgat aaaaaataca actacttatg
     6121 aaacctccat agataataga cgtttaaaat ttcaatcaaa aattgattcg ctctatttt
//
LOCUS       DAOTKS010000046         6104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_11926_length_6104_cov_4.855017, whole genome shotgun sequence.
ACCESSION   DAOTKS010000046 DAOTKS010000000
VERSION     DAOTKS010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6104
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11926_length_6104_cov_4.855017"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /gene="gldE"
                     /locus_tag="P5236_01590"
     CDS             <1..1254
                     /gene="gldE"
                     /locus_tag="P5236_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HRU63086.1"
                     /translation="LICSALISGSEVAFFSLSPQIKNEFEESNKKTEKKVLELINHPQ
                     RLLATILIGNNFVNVAIILLLFYFTNSIFDFSNAPVLGFIIETIVITFILLFFGEALP
                     KIYGAQYPKKMTLFTASFISALEKIFRPVASLLINSTSMVNKKLEKLKKSEISIDELS
                     QALELTTDNKDEDKEMLEGVIKFGNTRVADIMTSRIDMVAIDIKTNFKELLDLVVSTG
                     YSRIPVYSGNRDNIKGILYIKDLLPHLDKPAHFRWQSLIRQAYYVPETKKIDDLLNEF
                     QKNKVHFALVVDEYGGISGMVTLEDILEEIIGEISDEYDEEEKLYTKIDNHTFIFEGR
                     ISLNDFFKVTGIDEEEFSKVIKEVETLAGLILELKGEMPAKNEQIEYERYIFEILSIE
                     NRRIKKVKLHIKAAQDNLEENEKSV"
     gene            1350..1907
                     /gene="gldD"
                     /locus_tag="P5236_01595"
     CDS             1350..1907
                     /gene="gldD"
                     /locus_tag="P5236_01595"
                     /inference="COORDINATES: protein motif:HMM:TIGR03512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HRU63087.1"
                     /translation="MGGVLVLFISCNRKQTPRPYGYFRVDLPSISYRMLDTLGFPYRF
                     EISSHANVAIKKPKQVDEKYWIDINYPDLNATIYCSYKSIHGNFNSLSEDSRKIVYKH
                     TVRADAIQEKVYENPSRKVYGILYELKGNTASPVQFVLTDSTRHFFRGALYFNNVPNK
                     DSIAPMLDFVRKDIIKLMESFEWKP"
     gene            1907..2554
                     /locus_tag="P5236_01600"
     CDS             1907..2554
                     /locus_tag="P5236_01600"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HRU63088.1"
                     /translation="MHKYFKTREGVILIWQVTESKEELFQQLDGQTIDLLEYEKLKNE
                     RRQVEYLGTRIALKKLLNKEINLYHTSEGKPYLEDECYQISISHSQQWIAVIAHPNLS
                     VGIDIECFQPKILKLYERFLNDEELKYFGDEKNIRKLTLIWSAKEVIYKIIGNEAVNF
                     SHQIQVLPFKLKESGEIKTLHIPSEKIFRLHYNQTNDYVVVYGAAEKTYKEKIEK"
     gene            2602..3360
                     /locus_tag="P5236_01605"
     CDS             2602..3360
                     /locus_tag="P5236_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HRU63089.1"
                     /translation="MKNLYEKILFNRKNNHKMLAVLLDPDQCQGSLLSAILDTLKKGI
                     PDFIFVGGSHLNSSVDNLIATLKKEIEVPIVLFPGNASQFSQEADALLYLSLVSGRNA
                     EYLIGQHVISSFAIKQSGIEVISTAYLLIDGGKVSAVEYMSNTRSIPREKKEIVVSTA
                     LAGELLGMQMIYLEAGSGAIFPVPKELISYVKLFASIPIIVGGGIQTIEKLKECFDAG
                     ADIVVVGNLFEIYPNKIQDFVHFTHQYNEKTTLV"
     gene            complement(3352..4566)
                     /locus_tag="P5236_01610"
     CDS             complement(3352..4566)
                     /locus_tag="P5236_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRU63090.1"
                     /translation="MTETGQKDKLWSSSFISICIGNFLLFFAFYLLLPILPLYLMEEF
                     NSNKTTIGLILSSYTIAALLIRPVAGFFLDMFPRRPIYLVSYFLFSLCFIGYPLVTLL
                     NFFLFLRILHGLTFGMVSTAGNSLVIDIMPASRRGEGIGYFGIANNLAMSIGPMISLL
                     LHDHASYRFIFSISIVSALLGFISATTVKSKKKVERLANQPIVLDRFFLLKGTHAGIC
                     LTLMAIPYGMLTTYVAIYGKETGFQGGMGMFFTLLAIGLIFSRFFSGKMVDKGFIVKI
                     IKIGTLICIVSVLILSSLNAFYGIYPSLAEGSFYAIAVFLGVGYGMLFPAYNTLFVNL
                     APNNRRATASSTFLTTWDVGIGLGLIIGGKIGDTQGGLSLSYFTGGIIAAISFVYFLK
                     IAGPHFEKNKLY"
     gene            complement(4573..5106)
                     /locus_tag="P5236_01615"
     CDS             complement(4573..5106)
                     /locus_tag="P5236_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HRU63091.1"
                     /translation="MIINERDTRRERLLQVANEMMTAARTAPKAKGYDIIEVAVISDE
                     TIQQLSDAMLEYSQKSGLKFITRDAENILHAQAIVLIGTKQKVQNLNCSYCGFSTCNE
                     KDKFPEIPCAINMVDVGIAIGSACSVAADRRVDTRVMFSVGRVAQEMELLPGCTSIYG
                     IPLSCSSKNPFFDRQKK"
     gene            complement(5132..5935)
                     /locus_tag="P5236_01620"
     CDS             complement(5132..5935)
                     /locus_tag="P5236_01620"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRU63092.1"
                     /translation="MKKSYEDLCLKVCKIAKETGKFIAGERISFDESKVELKGLHDLV
                     SYVDKTSEKYITEQLSKLLPEAGFIVEEGTIDQKGERFNWIVDPLDGTTNFVQGVPVY
                     AVSIALMDNNELVVGVVYEIGRNECFYTWKGGGSFLNDKPIHVSKRNRIQDALLATGF
                     PYSAFDRLDEYMKFLKWTFIETRGVRRLGSAATDLAYVACGRFDAFWEYNLKPWDMAA
                     GALLIQEAGGTLSDFKGGNNYLFGKEAVATNGLLDKIMLPKLQELMQIE"
BASE COUNT         2121 a         1025 c         1037 g         1921 t
ORIGIN      
        1 ttaatatgtt ctgctcttat ttcaggatca gaagtagctt ttttctcatt atctccacaa
       61 attaaaaatg aatttgaaga aagtaataaa aaaaccgaaa aaaaagtttt agaacttatc
      121 aatcatcccc aacgcttgct ggcaactatc ctaataggga acaatttcgt taatgttgct
      181 atcattttgt tgttgtttta ttttaccaat tctatttttg atttttccaa tgctcctgtg
      241 ttgggtttta ttatcgaaac cattgtaatt acttttattt tgctgttttt tggagaagct
      301 ttacctaaaa tttatggagc acagtatcct aaaaaaatga cactttttac agcttctttc
      361 atttcagcac tggaaaaaat tttccgtcca gtagccagtt tattgattaa ttcaacttct
      421 atggttaaca aaaaacttga aaaactgaaa aagtccgaga tttctattga tgaactttca
      481 caggcattag aattaacaac tgacaataaa gatgaagata aggagatgct tgaaggtgtt
      541 attaaatttg gcaacactcg tgtagctgat attatgactt cacgtattga catggtagca
      601 attgatatta aaaccaattt taaggaactt ttagaccttg ttgtttctac aggatattct
      661 cgtattccag tttattctgg caatcgcgat aatatcaaag gtattcttta tatcaaagat
      721 ttgttgcctc atctggataa acctgcccat tttcgctggc aaagccttat caggcaagca
      781 tattatgttc ctgaaacaaa aaagattgat gatttgctga atgaatttca gaaaaacaaa
      841 gttcattttg cactcgtggt tgacgaatat ggtggaattt caggaatggt tacactggaa
      901 gatattctgg aagaaattat tggtgaaatc agtgatgaat acgatgaaga agaaaaatta
      961 tatactaaaa tcgacaatca tacttttatt tttgaaggaa gaatatcgtt gaatgacttt
     1021 ttcaaagtga caggaattga tgaagaagaa ttctcgaaag taataaaaga agtagaaaca
     1081 ctggctggcc tcattctgga actgaaagga gaaatgcctg ccaaaaatga acagattgaa
     1141 tatgaacgtt atatttttga aattttatcg atagaaaaca gacgaataaa aaaagtaaaa
     1201 cttcatatca aggcagcaca ggataattta gaagaaaatg aaaaatcagt ttgaaatgat
     1261 tttaaccttc gtgtttccca tattaaatat tagatttgaa agtgaatgtt ggataaccaa
     1321 tgaataaaca tctcttaaaa acattgacga tgggtggagt acttgtactg ttcatttcct
     1381 gtaacagaaa acaaacacct cgcccgtatg gatattttcg cgtagattta ccttcaattt
     1441 cttacagaat gctggacacg ttaggttttc cctatcggtt tgaaatttca tcccatgcca
     1501 atgttgctat taaaaagcca aaacaagttg atgaaaaata ttggatagat attaattacc
     1561 cagacttgaa tgccactatt tattgtagct acaaatctat tcatggaaat ttcaatagtt
     1621 tgtcagaaga ttcacgtaaa atcgtttata aacatacagt tcgtgctgat gccattcagg
     1681 aaaaagttta tgaaaatcca tccagaaaag tatatggaat tctttacgag ttaaaaggaa
     1741 atactgcttc acctgtgcaa tttgttctga cagacagcac acgacatttt tttagaggag
     1801 ccttgtattt taacaatgtt cccaataaag attcgatagc tccgatgcta gattttgtac
     1861 gaaaagatat tatcaaattg atggaatctt ttgaatggaa gccatgatgc ataaatattt
     1921 taagacgcga gaaggggtta ttttgatttg gcaagtgaca gaaagcaaag aagaactttt
     1981 tcaacaattg gatggacaaa caattgattt actggaatat gaaaaactga aaaatgaaag
     2041 gcgtcaagtg gaatatttag ggacacgaat tgctttaaaa aaattgctga ataaagaaat
     2101 caatctttat catacttctg aaggtaaacc ttatctggaa gatgaatgtt atcaaattag
     2161 tatttctcat agtcagcaat ggatagctgt gatagcccat ccaaatcttt cagtaggaat
     2221 agacattgaa tgttttcaac caaaaattct gaaactctat gaacgttttt taaatgatga
     2281 agaattaaaa tattttggtg atgaaaagaa tatacgaaaa ttaaccttaa tttggtcagc
     2341 taaggaagtg atatataaga ttattggaaa tgaagcagtt aatttttctc atcaaattca
     2401 agttttacct tttaaattga aggaaagcgg agaaataaaa acactgcata ttccgtcaga
     2461 aaaaatattt cgactccatt ataaccagac aaatgattac gtagtggttt atggcgcagc
     2521 agaaaaaaca tataaagaaa aaattgaaaa atgatttttt tttcagccaa ctttttaatt
     2581 tattatggaa attgaaaaag catgaaaaat ttatatgaaa aaatcctttt caacagaaaa
     2641 aataatcata aaatgctggc agtgttgctt gatcctgatc aatgtcaggg aagtttattg
     2701 tcagccatac tggatacttt gaaaaaagga attcctgatt ttattttcgt aggtggtagc
     2761 catctcaact cttcggtgga caatctcata gctacgctga aaaaagaaat tgaagtgcca
     2821 attgttcttt ttcccggcaa tgcttctcaa ttttcgcaag aagcggatgc cttattatat
     2881 ctttccttgg tttcgggtag aaatgcggaa tatttgattg gtcagcatgt tatttcctct
     2941 tttgctataa aacaatcagg aattgaagta atttcaacag cttatttatt aatagatggt
     3001 ggaaaagtaa gtgcagtgga atacatgagc aacactcgct ccattccacg tgaaaaaaag
     3061 gaaattgtag tttcaactgc tttagctgga gaattattgg gaatgcagat gatttatctt
     3121 gaagcaggaa gtggagcaat tttcccggtt cccaaagaat taatttctta tgtaaaatta
     3181 tttgcttcta ttcctattat tgtaggtggt ggtattcaaa caatagagaa actaaaagaa
     3241 tgttttgatg cgggagcaga tatagtagta gtaggaaatt tatttgaaat ctatcccaat
     3301 aaaattcaag attttgtcca ttttactcat caatataacg agaaaaccac attagtataa
     3361 tttgtttttt tcaaaatgag gtcccgcaat tttcaagaaa taaacaaatg aaattgctgc
     3421 tataattcca cctgtaaaat acgataagga caatccacct tgtgtatcac caatttttcc
     3481 accaatgata agccccaagc caattccgac atcccatgtg gtcaaaaaag ttgagctggc
     3541 agtagctctt ctgttgttgg gggcgagatt gacaaacaaa gtgttataag ctgggaacaa
     3601 cattccataa ccgacaccta agaagactgc aattgcgtag aatgatcctt cagcaagtga
     3661 aggataaatc ccataaaagg catttaacga agacaaaata agaactgaaa cgatacaaat
     3721 taatgtacca atttttatta ttttcactat aaaaccttta tcaaccattt ttcccgaaaa
     3781 gaaacgtgaa aaaatcaaac caattgccag caatgtgaaa aacattccca tacctccctg
     3841 aaagcctgtt tcctttccat aaattgccac ataagtagtt aacattccat aaggtattgc
     3901 cattaaagta agacaaatcc cagcatgtgt tccttttagg aggaaaaaac ggtctaatac
     3961 aattggttga ttggctaaac gctcaacttt cttttttgac ttaacagtgg ttgcggagat
     4021 aaagcccaac aaagcactta caatggaaat ggagaaaata aaacgataag aagcatgatc
     4081 gtgtaataaa agactaatca tcggtccgat ggacattgct aaattattag caataccaaa
     4141 atagccaatt ccttcacctc ttcgagaagc tggcataata tcgataacca agctattacc
     4201 tgctgtcgag accattccaa aagtcaagcc atgcaaaatg cgaagaaaaa ggaaaaaatt
     4261 gagaagagtt accaacggat aaccgataaa gcataaagaa aataggaaat aagataccag
     4321 ataaattggt cttcgaggaa acatatccag aaaaaaacca gcaacaggtc gtatcaagag
     4381 agctgcaatg gtataagaag aaagaataag ccctatcgtt gttttgttgg aattgaattc
     4441 ttccattaag tataaaggaa ggataggcag cagtaggtaa aaggcaaaaa acagtaaaaa
     4501 atttccgatg caaatactga taaaactcga tgaccagagt ttatcctttt gacccgtttc
     4561 cgtcatttta tattattttt tctgacgatc gaaaaaggga tttttcgaag aacaactgag
     4621 agggattcca taaatagaag tgcaacccgg caaaagttcc atttcttgag ctacacgtcc
     4681 tacagaaaac attacacgag tatctactct tctatcggca gcaactgagc aagctgaccc
     4741 aatagctatc cctacatcaa ccatatttat agcacacgga atttcaggaa atttatcttt
     4801 ttcattacaa gtggagaaac cacaatagct acaatttaaa ttctgaactt tttgttttgt
     4861 cccaataagg acaatagctt gagcatgaag gatattctct gcatcacgtg taataaattt
     4921 caaaccactt ttttggctat attccaacat ggcgtctgat aattgttgaa tagtttcatc
     4981 gctaattacc gctacttcta tgatgtcata tccttttgct ttgggagcag ttctggcagc
     5041 cgtcatcatt tcatttgcca cttgaagtaa acgttcccgt cgagtatcac gctcattaat
     5101 tatcataatt caattttttt gacatttatt tttattcaat ttgcattaat tcttgcaatt
     5161 ttggcaacat aatcttgtca agcagaccat ttgttgctac tgcttctttt ccaaaaagat
     5221 aattattgcc acctttgaaa tcggagagtg ttcctccggc ttcttgaata agtaaagcac
     5281 ctgcagccat atcccatggc ttaagattgt attcccagaa tgcatcgaag cgtccacagg
     5341 ctacgtaagc caaatcggta gcagccgatc ccagccgacg aacacctctt gtctcaataa
     5401 atgtccattt taaaaatttc atgtattcat ccaatcgatc aaaagcagaa taaggaaagc
     5461 ctgtggcaag taaagcatct tgtatgcgat tacgttttga cacatgaatg ggtttgtcat
     5521 ttaaaaatga acctccgcct ttccatgtat aaaaacattc attacgtccg atttcatata
     5581 ctacgcctac gaccagttca ttattatcca tcaaagcaat gcttaccgca taaactggca
     5641 cgccctgtac aaaattagta gtgccatcaa gtggatcgac tatccagtta aaacgttcac
     5701 ctttttggtc gatagtacct tcttcgacaa taaaacctgc ttcgggtagt aatttactta
     5761 attgttcagt aatatatttt tcagaagttt tatctacata gcttaccaga tcgtgtaagc
     5821 cttttaattc aactttgctt tcatcaaacg atatgcgttc ccctgcaata aattttcctg
     5881 tttcttttgc tattttacaa acttttagac aaagatcttc gtaacttttt ttcatcattc
     5941 attatccttt attaaaattt ttgcaaaatt agggttttct aaaaaaataa gcaagccaca
     6001 ttttttaatt attttgtcga ttagagaaat taatttttga gatattcaaa aataagttaa
     6061 taaggtttga ttgttttgtt tgtcataagt tattaaggtt ttaa
//
LOCUS       DAOTKS010000047         6101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_11928_length_6101_cov_4.316077, whole genome shotgun sequence.
ACCESSION   DAOTKS010000047 DAOTKS010000000
VERSION     DAOTKS010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6101
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11928_length_6101_cov_4.316077"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(292..963)
                     /gene="pssA"
                     /locus_tag="P5236_01625"
     CDS             complement(292..963)
                     /gene="pssA"
                     /locus_tag="P5236_01625"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HRU63093.1"
                     /translation="MKKNIPNFITCLNLFSGCIAVYFAFQGNYAIVLLFVFIAAFFDF
                     LDGLMARLLNAPSALGKELDSLSDIVSFGLTPGAIAFSLLQQTNLPFWLAFTGFLLPI
                     FSALRLAKFNLDTRQTNYFLGLPTPAHAIFWAGLSYSFSFFLIENYWITLTLIVLFSG
                     LLVSEIPMFSLKFKTLRWEGNEIQYIFILVSIVLFAVFLLDSFSLIICWYILLSLLTP
                     KIFKE"
     gene            complement(987..1442)
                     /gene="dtd"
                     /locus_tag="P5236_01630"
     CDS             complement(987..1442)
                     /gene="dtd"
                     /locus_tag="P5236_01630"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HRU63094.1"
                     /translation="MRVLIQRVDEALVTVDGEKIAAIGKGLLAFVGIEENDTEKDAEW
                     LAGKTASLRIFDDEKGIMNLSVMDINGEILVVSQFTLCAMTHKGNRPSYVKAAKPEKA
                     IPLYEFFCQTLSTKIGKQVKTGIFGAHMKIALVNNGPVTIWIDSKNPLS"
     gene            complement(1489..2589)
                     /gene="ychF"
                     /locus_tag="P5236_01635"
     CDS             complement(1489..2589)
                     /gene="ychF"
                     /locus_tag="P5236_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRU63095.1"
                     /translation="MGLQCGIVGLPNVGKSTLFNCLSNTKAQAANFPFCTIEPNIGVI
                     TVPDKRLNKLAELVHPQKIVPTTVEIVDIAGLVKGASKGEGLGNKFLANIRETDAILH
                     ILRCFDDENVAHVNGKVDPVRDKEIVDMELQLKDLETIESRINKVQKQAQTGGDKQAK
                     RICEILLQYREALLQGKSARTVKLDKEEKKLVKDLYLLSDKPVMYVCNVDENSIHNEN
                     AYVKAVRESIKDENAEILVICARLEAEIAELETYEERQMFLEEWGLTESGVSRLIKSA
                     YRLLNLETFFTVGPQEVRAWTYEKGWKAPQCAGVIHTDFEKGFIRAEVIKYDDFIRLG
                     SEAACREAGKIYIEGKDYEVQDGDIMHFRFNV"
     gene            complement(2683..3311)
                     /gene="purN"
                     /locus_tag="P5236_01640"
                     /pseudo
     CDS             complement(2683..3311)
                     /gene="purN"
                     /locus_tag="P5236_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307440.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
     gene            3411..3647
                     /locus_tag="P5236_01645"
     CDS             3411..3647
                     /locus_tag="P5236_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004347843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRU63096.1"
                     /translation="MSEVEEKVKAIIAEKLGVDEAEITPSASFTNDLGADSLDTVELI
                     MEFEKEFGISIPDDQAEKITTVGDAINYIENLKK"
     gene            3661..4923
                     /gene="fabF"
                     /locus_tag="P5236_01650"
     CDS             3661..4923
                     /gene="fabF"
                     /locus_tag="P5236_01650"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRU63097.1"
                     /translation="MELKRVVVTGLGAVTPVGTTVPIFWNNIVNGVSGAAPITHFDAS
                     QFKTQFACEVKDFDPTQYFEKKEIRKMDLYTQYGLVAAKEAVENCGVDVEKVDKNEIG
                     VIFSSGIGGIATYEQEVSNYYLNKDAGPKFNPFFIPKMISDIAAGQISIQYGFHGPNF
                     ATTSACASSTNSLIDAFNYIRLGKAKMFVVGGAEAAITPAGIGGFNAMMAISTRNDDP
                     KTASRPFSKSRDGFVMGEGAGCLILEELDFALARGANILCELVGGGLSADAYHLTASH
                     PDGLGAILAMSNALKDAELSPTDIDYINVHGTSTGVGDISEVKAIKEVFGEHAYKLNI
                     SSTKSMTGHLLGAAGAVEAIISILSLINGIVPPTINFTEGDEDPEIDYRLNFTFNQAQ
                     KRELNAVLSNNFGFGGHNATIIFKKYTK"
     gene            4928..5686
                     /gene="rnc"
                     /locus_tag="P5236_01655"
     CDS             4928..5686
                     /gene="rnc"
                     /locus_tag="P5236_01655"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRU63098.1"
                     /translation="MINLFSKKLRLFSRKRKEPYSLFYKVLGFYPHNIDYYQLAIRHK
                     SAPIITKNGKILSNERLEFLGDAVLNSIISDILFLHYKNKDEGFLTTTRSKIVNRFTL
                     NRLSLELELQNLVKIAKHVNVNNINDIYGNALEALIGAIYLDKGYEDSKQFVKKRLLC
                     LLDLEKLEKEEINYKSKLIEWCQKNKIPIEFEVVSENLSKDNKHVFHSHVLIDGKIIS
                     ESEGYSKKESQQNASRLAYEQIISRLHVQKQEIF"
     gene            5806..6006
                     /locus_tag="P5236_01660"
     CDS             5806..6006
                     /locus_tag="P5236_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRU63099.1"
                     /translation="MLTEKIGTNAGIIWNILHENGEMEVKALKKAAKLTEKDLNMALG
                     WLAREGKIQFNEQENELFVSLI"
BASE COUNT         2097 a          992 c         1008 g         2004 t
ORIGIN      
        1 taaaaattat tcaaaaattc ttttcttaaa aaattgttat caataaaata aaccaaataa
       61 taaaaattac aagaaacaaa agtactaaaa aatactgatt ctgtttctaa cctcaaagta
      121 ctttgatggt tatactttgt tactcatttt tataaagtta acaaaattag aataaaggta
      181 agagaaagtt ttttattctt tattttttta ttaattcagt ttatcgagtt gagaagcaat
      241 taacataaag gaaatattac attttttctt ttttaggtga taatcagtaa attattcttt
      301 aaaaattttt ggtgtcaaca aggaaagcaa aatataccag caaataatga gtgaaaaaga
      361 atccagcaga aaaacagcga aaaggacaat acttaccagt ataaaaatat attgaatttc
      421 attcccttcc catcttagcg tcttaaattt taaagaaaac ataggaattt cacttaccaa
      481 taatccactg aaaagaacaa ttaatgttaa tgtaatccag taattttcaa tgagaaaaaa
      541 agaaaatgaa taacttaatc ctgcccaaaa aatagcatga gctggtgtag gtaatcctaa
      601 aaaataatta gtttggcggg tatccaggtt gaatttagct aatcgcaaag ctgaaaaaat
      661 aggaagcaaa aaaccagtaa aagccagcca gaaaggcaaa ttggtttgtt gaagtaaaga
      721 aaaagcaatg gcgcctggtg tcaagccaaa actgacaatg tcggataaag agtctaattc
      781 ttttcctaaa gcagaaggag catttagcaa acgtgccatt aagccatcta aaaaatcaaa
      841 aaatgctgca atgaaaacaa agagcaaaac tatagcataa tttccctgaa aagcaaaata
      901 tacagcaata catcccgaaa ataaattcag acaggtaatg aagttaggaa tattcttttt
      961 catacttttt gaaaataagt gtttatttat gaaagaggat tttttgaatc gatccagatg
     1021 gtcaccggtc cattattgac taaggcaatt ttcatgtgag ctccaaaaat gcctgttttt
     1081 acttgtttcc caatttttgt gcttaatgtt tgacaaaaga attcatacaa gggaatggct
     1141 ttttctggtt ttgctgcttt tacatacgaa ggtcgattgc ctttgtgagt catggcacaa
     1201 agagtaaact ggcttacaac cagaatttct ccatttatat ccattacgga taaattcatt
     1261 attcctttct catcatcaaa gattcgtagc gaggctgttt ttccagccaa ccattctgca
     1321 tctttttcag tatcgttctc ttcaatcccg acaaaagcta aaagtccttt cccaatagca
     1381 gcaatttttt caccatcaac tgtcaccaat gcttcatcta ctctttgaat caaaactcgc
     1441 atataagatt tatatttaaa gcgaatctaa attgctttta aaaaacattt aaacattgaa
     1501 tcgaaaatgc atgatatcgc catcctgaac ctcataatct tttccttcga tgtaaatttt
     1561 cccagcttca cgacatgctg cttctgaacc taaacggata aaatcatcat attttatgac
     1621 ttctgcccga atgaagcctt tctcaaaatc ggtatgaata actccagcac attgaggagc
     1681 tttccaacct ttttcataag tccaagcacg cacttcttgt ggacctactg taaagaaagt
     1741 ttccagattc aataatcggt aagccgattt aatcaatcgt gaaacgcctg attctgttaa
     1801 tccccattct tccaggaaca tttgacgttc ttcataggtt tccagttcag caatttcggc
     1861 ttccaatcga gcacagatga ctaaaatttc cgcattttcg tcttttattg attcacggac
     1921 agcttttacg taagcattct cgttatgaat ggaattttca tctacattgc aaacatacat
     1981 tactggcttg tcgctcagta aataaagatc tttgacaagt tttttttctt ctttatccag
     2041 tttaacggtg cgtgctgatt tcccttgcag taaagcttcg cgatattgaa gtaaaatttc
     2101 acagattctt ttagcttgct tatcaccacc tgtttgagct tgtttttgta ctttattaat
     2161 tctgctttca atggtttcca aatctttcaa ctgaagttcc atatctacaa tttctttgtc
     2221 tcgaacagga tcaacttttc cattcacatg agcaacattt tcatcatcaa agcatctcaa
     2281 tatatgtaat atagcgtctg tttcacgaat attggctaaa aatttattcc ccaagccttc
     2341 tcctttgctc gctcctttta ccaatcccgc aatgtccaca atttctacag tagtaggtac
     2401 aattttttgg ggatgaacca gttcagctaa tttgttgagg cgcttatctg gtacggtaat
     2461 cactccgata tttggttcga ttgtacaaaa aggaaaattg gcagcttgag cttttgtatt
     2521 tgataaacaa ttgaataaag tagattttcc cacattaggc aaacctacaa ttccacattg
     2581 aagacccatg aaataaaaaa ttagttggta aaatcttaag caatatttca ttgcaaagat
     2641 acataaaatc tctcaaagct ttttgttcaa ataataatgg aattagtttt gctgacccca
     2701 gattttttca atcactcgag gataatattg atattctaat tcatgaacct tttttgcaat
     2761 ggtttcaggt gtatcttcgg gaagcacagg acattttgct tgaaaaatga tttcacctgc
     2821 atcatattca ttattcacgt aatgaatagt gatacctgtt tcggtttctc ctgcttcttt
     2881 tactgcctcg tgaactcgca ttccatacat tccttttcca ccaaattttg gaagcaaagc
     2941 aggatgaata ttaatgattt tttgtggata agcatctatc accagcaatg gaacttgaag
     3001 taagaaacct gcaagaacta tagcatctat cttttgctct tgtaaaaaat tcaatatttt
     3061 ttctcctttt aaaaaatctt cttttgaaaa cgtaacagaa ggtactccaa gtttttttgc
     3121 tcgttcatga acaaaagctt tttcattatt cgaaagaata acaggaaaaa caaaattggg
     3181 atgattagaa aaatatcgaa taatattttc ggcatttgaa ccagaacctg aggcaaaaat
     3241 tgcaattcta atgggcataa ttttaagtta aatactgcac aaaattaggc aaattgtgca
     3301 aaaaagtgca tttttttcaa taaaacgatg gttatttgaa gaaaatttta tttctttgca
     3361 gcacaaaatt ataaaacaaa ttttatttat aaacttaaaa cttaaaaatt atgtcagaag
     3421 ttgaagaaaa agtaaaagcg attattgccg agaagttagg cgtagatgaa gctgaaatta
     3481 caccttcagc aagttttaca aatgatcttg gtgcagattc gttagatacc gttgaactca
     3541 ttatggaatt tgaaaaagaa tttggtatct ctattcctga cgatcaagca gaaaaaatta
     3601 caacagttgg tgatgctatc aactatattg aaaatttgaa gaaataataa gttttctctt
     3661 atggagttaa agagagtagt tgtaacagga ttaggagcgg ttacaccagt gggaacgaca
     3721 gttcccattt tttggaacaa catagttaat ggtgtaagtg gagctgcgcc tattacccat
     3781 tttgatgctt ctcaatttaa gacacaattt gcttgcgaag taaaagattt tgatcctacc
     3841 caatattttg agaagaaaga aattcgcaaa atggatcttt atactcaata tgggttagta
     3901 gcagccaaag aagctgtaga aaattgtggg gtagatgtag agaaagtcga taaaaatgaa
     3961 attggggtga ttttttcttc tggaatagga ggcattgcca cttatgaaca agaagtgtct
     4021 aattattatc tcaataaaga cgctgggcca aaattcaatc cattttttat tccaaaaatg
     4081 atttccgata ttgcagcagg acaaatttct attcaatatg gttttcatgg tcctaatttt
     4141 gctaccactt ctgcttgtgc ttcgtcaacc aattcattga ttgatgcttt taattatatt
     4201 cgtttaggaa aagccaaaat gtttgttgtt ggaggtgctg aagcagctat tacaccagca
     4261 ggcattggcg gatttaatgc tatgatggca atatcaacca gaaatgacga tccaaaaact
     4321 gcttcacgac cttttagcaa aagtcgtgac ggttttgtca tgggagaagg agctggttgt
     4381 ttgattttgg aagagcttga ttttgcactt gctcgcggag caaacatttt atgtgaactc
     4441 gtaggtggag gtttatcagc tgatgcttat catttaacag cttctcatcc agatggtttg
     4501 ggagctatcc tggcaatgag taatgcttta aaagatgcag aattatctcc aactgatatt
     4561 gattatatta atgttcatgg tacttctaca ggcgttggtg acatttcaga agttaaggca
     4621 attaaagaag tttttggcga acatgcttat aaactgaata ttagttcgac aaaatctatg
     4681 acggggcatt tattaggtgc agctggagcg gttgaagcaa tcatttctat actttctcta
     4741 ataaatggaa ttgtaccacc taccattaat tttacggaag gcgatgagga tccagaaatt
     4801 gattatcgtt tgaactttac atttaatcaa gctcaaaaaa gagagctcaa tgctgtttta
     4861 tccaataatt ttggctttgg tggacataat gcaacaatca ttttcaaaaa atatactaaa
     4921 taagcttgtg ataaatcttt tttcaaaaaa attaaggctc ttttctcgca aaagaaagga
     4981 gccttattct ttattttata aggttttagg attttatccc cacaacattg attactatca
     5041 actggcaata cgacataaat cggctccaat cattaccaaa aacggcaaaa tattaagcaa
     5101 cgagcgtttg gaattcttag gagatgctgt gttaaattct atcatctcag atattctttt
     5161 ccttcattac aaaaacaaag atgaaggatt tcttactact actcgttcta aaattgtaaa
     5221 tcgatttact ttaaatcggt tatctcttga attggaactg caaaatttgg tgaaaatagc
     5281 taaacatgta aatgtcaaca atattaatga tatttatggg aatgcattag aagctttaat
     5341 tggcgctatt tatcttgata agggttacga agatagcaaa caatttgtta aaaaaagatt
     5401 actttgtttg ttggatttgg aaaaacttga aaaggaagaa ataaattata aatctaaatt
     5461 aattgaatgg tgtcagaaaa ataaaatacc cattgaattt gaagttgtta gtgaaaattt
     5521 aagcaaagac aataaacatg tttttcattc tcatgtgttg attgatggaa aaattatttc
     5581 tgaatcagaa ggctatagta aaaaagaatc acaacaaaat gcttccagac tggcttatga
     5641 gcaaataatt tcgcgtctcc atgttcagaa acaagaaatc ttttaaaatg aaattattca
     5701 taatgagggc tttagttctt cgatcttttg attttgatat tcatggtatt tttaataact
     5761 ttgcaaaata aaacttataa ttaacaatta aaaaaccgaa ccactatgtt aacagaaaaa
     5821 attggaacaa atgctggcat tatatggaat attctccatg aaaatggaga aatggaggta
     5881 aaagccttga aaaaggcagc taaattaact gaaaaagatt taaatatggc tttaggttgg
     5941 ttagctcgtg agggaaagat tcagtttaat gaacaagaaa atgaattatt tgtaagtctt
     6001 atttgagcga ggaaattaag gcggttctgt tgatgtcccg cctttttaat tttaaaatta
     6061 ctttcaattt ttatcccaaa aaatttcata aacgaaaaaa t
//
LOCUS       DAOTKS010000048         6009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_12105_length_6009_cov_4.014444, whole genome shotgun sequence.
ACCESSION   DAOTKS010000048 DAOTKS010000000
VERSION     DAOTKS010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6009
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12105_length_6009_cov_4.014444"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(361..609)
                     /locus_tag="P5236_01665"
     CDS             complement(361..609)
                     /locus_tag="P5236_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63100.1"
                     /translation="MNEMQVKLKAEKIIEALTALSLGKKPNVFSSEIFKTLANHPNFS
                     AIRDAYIEYLKDFNGEVNSVDEVKRLFDFRVKILELFS"
     gene            complement(662..2410)
                     /gene="aspS"
                     /locus_tag="P5236_01670"
     CDS             complement(662..2410)
                     /gene="aspS"
                     /locus_tag="P5236_01670"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRU63101.1"
                     /translation="MYRTHTCGELRLTNVGQKTTLAGWVQRIRKMGGMVFVDLRDRYG
                     ITQLVFNQQINENLWKEANELGREFVIQATGEIAERSNKNLNLPTGEIEILVEELNIL
                     NESKTPPFTIEDNTDGGEDLRMKYRYLDLRRNIIRQNLELRHQLTIETRRYLDQLGFI
                     EVETPALIASTPEGARDFVVPSRMNPGEFYALPQSPQLLKQLLMVAGFDRYFQIVKCF
                     RDEDLRADRQPEFTQIDCEMSFVTQEDVLQTFEGLIKYLFKTLKNVDFIQPFPRMRWA
                     DAMKLYGSDKPDIRFGMTFVELKDVVQGHQFSIFDNGEYVGGICVTGCADYSRKQLDE
                     LTEFVKRPQIGAKGLIYIKYEKDGTFKSSIDKFFNNEDLKKIASQFQSQAGDLILILA
                     GEELKTQKALCELRLEMGERLGFRDKNVFAPLWVVDFPLLEWDDELQRYKAMHHPFTS
                     PKLEDIPLLETDPGAVRANAYDLVINGVELGGGSIRIHDSKLQDQMFKVLGFTEEKAQ
                     AQFGFLLNAFQYGAPPHGGLAFGLDRFVALLNGLDSIRDCIAFPKNNAGRDVMINAPA
                     PLDESQLEELHLTIKL"
     gene            complement(2442..3314)
                     /gene="fabD"
                     /locus_tag="P5236_01675"
     CDS             complement(2442..3314)
                     /gene="fabD"
                     /locus_tag="P5236_01675"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRU63102.1"
                     /translation="MKAYVFPGQGAQFVGMGKDLYEQSEIAKKYFEKANEILGFRITD
                     LMFEGTDEDLRQTKVTQPAIFLHSVISAFVLEDNFKPDMVAGHSLGEFSALVAAQALT
                     FEDGLILVSKRAQAMQKACEMEESTMAAIIGLDDETVEEVCDSIKDEIVVPANYNCPG
                     QIVISGTIRGVEKACELLKEKGAKRALKLSVGGAFHSPLMESARIELSQAIHQTSFST
                     PICPVYQNVNAYPQTNPENIKQNLIAQLTAPVRWTQTIKNMINDGATEFIELGPGDVL
                     KGLIKKINPNVMVR"
     gene            3576..4202
                     /gene="rsmG"
                     /locus_tag="P5236_01680"
     CDS             3576..4202
                     /gene="rsmG"
                     /locus_tag="P5236_01680"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008022144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRU63103.1"
                     /translation="MDLILKYFPHLTPTQISQFEALYGLYSDWNQKINVISRKDIENL
                     YEHHVLPSLGIAQVIHFQKGTSILDVGTGGGFPGIPLAILFPEVNFTLIDSVGKKIKV
                     ASEISKAIALENTVFKQIRLEEEKGQYDFIVSRAVMTMNELVKQGSKNILKRQINALP
                     NGIICLKGGEISNEIRNYKNIAEIFPLSNYFKEEYFKTKKIIYMPVFK"
     gene            4226..4873
                     /locus_tag="P5236_01685"
     CDS             4226..4873
                     /locus_tag="P5236_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRU63104.1"
                     /translation="MKIKKFVFNPFEENTYIVSDETKEAIVIDAGCLDKEEKRLLKEY
                     IADNQLIIKYILNTHLHIDHQFGNRFLLENYFIAPLAGKEDEFFLDTFREQTYFFGIK
                     VQEEPAPLGGYLIDNQKINFGNTSLQVFHVPGHSPGSMAFYFENEKAIFTGDVLFKGT
                     IGRTDFEKGDFDTIIRSIIQRLLPLPDETVVYSGHGPITTIGNEKKYNPYLQNYC"
     gene            complement(4864..5469)
                     /gene="nadD"
                     /locus_tag="P5236_01690"
     CDS             complement(4864..5469)
                     /gene="nadD"
                     /locus_tag="P5236_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HRU63105.1"
                     /translation="MNTQTENTNKKIGLYFGSFNPVHLGHVNLAQYIIDNQYVDEVWF
                     VVSPHNPLKEDTELVDENIRLKMVELAIQNHPQLKVSDLEFSLPKPSYTINSLHAFSE
                     KYPENKFYLLIGSDNVLIFDQWKDYQKILEEYPVLVYPRKNFDASHLLSKYPQMKFLS
                     TPYYDISSSTIRQALKEQKDVDKWLHPAVYQFIIENKLYQQ"
     gene            complement(5482..>6009)
                     /gene="gmk"
                     /locus_tag="P5236_01695"
     CDS             complement(5482..>6009)
                     /gene="gmk"
                     /locus_tag="P5236_01695"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRU63106.1"
                     /translation="AGKSTLVHHLLNSNLNLEFSVSATSRAPRLNEKEGIDYYFLTPE
                     EFKQKIANGEFLEYEEVYPDCFYGTLRSEIDRITQKDKNVIFDVDVKGGLNIKRQFGN
                     QALAIFVAPPNMEALKERLLKRGTETPEMIQKRIEKAAYEMSFAPQFDKIIINDDLEK
                     AKKEIEETVRDFLNK"
BASE COUNT         1992 a         1022 c          916 g         2079 t
ORIGIN      
        1 tcttcaaaga gtagtatagt cgtgttcttt aaaaatctat taagatatcg gaatttagct
       61 catgaagccg aatatccaga tataggtcat tatacggata atcattataa ataataaaat
      121 tatcaaaatt ttttgcttgt acaggcgcgt acatttgcca ttgcaaataa ataaaaagtc
      181 ctgctgccga acgattgtat cgtgtggcat actaataaat gcaagtttaa aataaagcat
      241 cacataaaaa aggaaaaagt tactgaaata aattttctat atagttttcc tataatttat
      301 attatgtcaa ataggataaa atctcaatat actttcattt atctgttact tctaaccgat
      361 tcatgaaaac aattctaata tttttactcg gaaatcaaac aatcgtttta cttcatctac
      421 tgaattaact tcaccattga aatctttcag atattcaata taagcatcac gaatagccga
      481 aaaatttgga tgattcgcta atgttttgaa aatttcactt gaaaagacat tgggtttttt
      541 gcctaagctt aaagctgtga gggcttcaat aattttttca gctttcagct tcacctgcat
      601 ttcattcata aatcaacgaa tttaaattaa ttagttgaat atcaagtaat taatgttata
      661 attataattt tattgttaga tgcaattctt ccagttggct ttcatctaat ggtgctggag
      721 cattgatcat tacatctctt ccagcattat ttttagggaa agcaatacaa tcgcgaatag
      781 aatccaatcc atttaataaa gcaacgaatc tgtccaatcc aaaagccagt ccaccatgtg
      841 gaggagctcc gtattgaaaa gcattcagta aaaagccaaa ttgagcttga gctttttctt
      901 cggtgaaacc aagcacttta aacatttgat cttgtaattt actgtcgtga atacgaatag
      961 aaccacctcc caattctaca ccatttatta ccaggtcata agcatttgct cttactgctc
     1021 ctggatcagt ttccaacaaa ggtatatctt ccaattttgg tgaggtaaaa ggatgatgca
     1081 tagccttata acgctgtaat tcatcatccc attctagtaa aggaaaatct actacccaca
     1141 aaggagcaaa cacattttta tcacgaaagc ctaacctctc tcccatttcc aaacgcaatt
     1201 cgcaaagagc tttttgtgtt ttcaattctt cgcctgccag aataagaata aggtcacctg
     1261 cttgagattg aaattgagaa gcaattttct ttaaatcttc attattaaaa aatttgtcaa
     1321 tagaagattt gaaggttcca tctttttcat atttaatata aatcagtcct tttgctccaa
     1381 tttgagggcg tttgacaaac tctgtcaatt catcaagctg ctttctgctg taatccgcac
     1441 aacctgtgac acaaattccg ccaacatatt caccattatc aaaaatagaa aattgatgtc
     1501 cttgaacaac gtctttcaat tcaacgaatg tcattccaaa acgaatatcc ggcttatcag
     1561 aaccatacag tttcatagca tcagcccatc tcattcttgg aaatggttga ataaaatcaa
     1621 catttttcag cgttttaaaa agatatttaa tcaacccttc aaaagtttgc aacacatctt
     1681 cttgtgttac aaaagacatc tcacaatcta tttgtgtaaa ttcaggttga cgatcggctc
     1741 gcaaatcttc gtcgcgaaag cattttacaa tttgaaaata tcgatcaaaa cctgctacca
     1801 tcaataattg tttcaaaagt tgtggcgatt gcggtaacgc ataaaattct ccaggattca
     1861 ttcgtgatgg taccacaaaa tctcgtgcac cttctggtgt agaagcaatt aaagcaggtg
     1921 tttctacttc aatgaaaccc aactggtcga gataacgtcg agtttcaata gttaattgat
     1981 gacgtaattc aaggttctgt cgaataatgt ttcgtcgtaa atccaaatat ctgtatttca
     2041 tcctcaagtc ttcgccacca tctgtattgt cttctatggt gaatggcggt gtttttgatt
     2101 cattcaatat attcaattcc tcaacgagga tttctatttc gccggttgga agattcaaat
     2161 ttttattgct tcgttctgct atttctcctg ttgcttgaat gacaaattcg cgccccaatt
     2221 cattagcttc tttccagaga ttttcgttaa tctgttgatt gaaaactaat tgtgttattc
     2281 cgtatcgatc tcgaaggtca acaaaaacca ttccccccat ttttcgaatg cgttgtaccc
     2341 agcctgccaa agtcgttttt tgaccaacat tggtgagacg taattcacca caagtatgtg
     2401 ttctgtacat tttttataat aataattaat ttttttaaaa attatctcac catcacattt
     2461 ggattaattt ttttaatcaa accttttagt acgtcgccag gacctaattc tataaattct
     2521 gtagccccat cattaatcat gtttttaatg gtttgtgtcc atcgaacagg tgcagttaac
     2581 tgagcaatga gattttgctt gatattttca ggattggttt gaggataggc atttacgttc
     2641 tgataaactg ggcagatagg tgtagaaaaa gaagtttgat gaatagcttg gctaagttca
     2701 attctggccg attccatcaa aggtgaatga aaagctccgc caacactaag tttcagcgcg
     2761 cgtttagctc ctttttcttt taataattca catgcttttt ctactcccct tattgttcct
     2821 gaaataacga tttgtccggg gcaattataa tttgcaggta caacaatttc atctttgatc
     2881 gaatcgcaaa cttcttctac agtttcgtca tctaaaccaa tgatggccgc catagttgat
     2941 tcttccattt cacaagcttt ttgcatggct tgtgcacgtt ttgaaaccaa aatgagtcca
     3001 tcttcaaaag tcaaagcttg agctgccaca agagctgaaa attcacctaa tgaatgtcct
     3061 gctaccatat caggtttgaa attatcttct aaaacgaaag ctgaaattac tgaatgcaaa
     3121 aatatggctg gttgagttac tttggtttgt cgtaaatctt catcagttcc ttcaaacatt
     3181 aaatcagtaa tgcgaaatcc caaaatttca ttggcttttt caaaatactt ttttgctatc
     3241 tcagattgct cgtataaatc cttacccatg cctacaaatt gggctccttg accaggaaaa
     3301 acgtatgctt tcatttttga taaatttata aacagttaat tatattaatt ctttaaaaaa
     3361 tcttattaaa aagatttaaa aacgaaatat taagaagagt ttggcaaagt taataaaaaa
     3421 gatatatgta aacttcaatg tgaagattta actataaaca aattaaaaaa tagatttata
     3481 aattattgtt tttcaattat ttagaaatta tattaaattg gatttttact ttttaataaa
     3541 aaacctataa ttttgaaagg taaaacattg aagatatgga tttaatttta aaatattttc
     3601 ctcacctcac tcctactcaa atttcccaat ttgaagcact ttacggactt tattctgatt
     3661 ggaatcaaaa aattaatgtc atttcacgga aagatataga aaatttatat gaacatcacg
     3721 tattaccttc attaggaatt gcacaagtaa ttcattttca aaaaggaact tccattctgg
     3781 atgttggtac aggtggcggt tttccaggaa ttccacttgc tattttgttc cctgaggtaa
     3841 attttacctt gattgattca gtcggaaaaa aaattaaggt agcttcggaa atttcaaaag
     3901 caattgccct tgaaaataca gtttttaaac aaataagatt agaagaagaa aaaggtcaat
     3961 atgattttat tgtgagtcgt gctgtaatga cgatgaatga attggtaaaa caaggtagca
     4021 aaaacatctt aaaacgccaa ataaatgccc ttcctaacgg tattatttgc ttgaaaggtg
     4081 gagaaatttc taatgaaatt cgtaactata aaaatattgc tgaaattttt ccactttcca
     4141 attatttcaa agaagagtat tttaagacta aaaaaataat ttatatgcct gtttttaagt
     4201 aaaatatttt aacgagtgga aaaatatgaa aatcaaaaaa tttgttttta atccttttga
     4261 agaaaatact tatattgttt cagatgaaac caaagaagct atcgttattg atgctggctg
     4321 tttggataaa gaagagaaaa gattattaaa agaatatata gctgacaatc agctaattat
     4381 aaaatatatt ttaaatactc atttgcatat tgatcatcag tttgggaatc gttttttact
     4441 tgaaaattat tttattgctc ctttagctgg caaagaagac gaattttttt tagatacttt
     4501 tagagaacaa acttattttt ttggaattaa agttcaagag gagcccgctc cacttggtgg
     4561 atatttaatt gataatcaaa aaataaattt tggaaatact tctttacagg tttttcatgt
     4621 gccaggccat tcaccaggaa gtatggcttt ttattttgaa aatgaaaagg caatttttac
     4681 gggtgacgta ctttttaaag gaactattgg aagaactgat tttgaaaaag gagactttga
     4741 tactattatt cgttctatta tacagcgttt acttccactt cctgacgaaa ccgttgttta
     4801 ttctggacat ggacctatta ctaccattgg aaatgaaaaa aaatataatc cttatctaca
     4861 aaattattgc tgatataatt tattttcgat aataaactga taaacagcag gatgtagcca
     4921 tttgtcaaca tctttttgct cttttaaagc ttgtcggata gtagaagaag aaatatcata
     4981 ataaggtgtg gaaagaaatt tcatttgagg gtatttggaa agtaaatgag aagcatcaaa
     5041 atttttcctg ggataaacaa gtaccggata ttcttccaga attttttgat aatctttcca
     5101 ttgatcgaaa attaaaacat tgtcgctccc gatcagcaaa taaaatttat tttctggata
     5161 tttttcagaa aaagcatgta aactgttgat ggtataagac ggtttaggta aagagaattc
     5221 caaatcagaa acttttaatt ggggatggtt ttgaatagct aattcaacca tcttcaaccg
     5281 aatattttca tcaactaatt ctgtgtcctc ttttaaagga ttgtgaggtg aaacaacaaa
     5341 ccaaacttcg tcaacatatt gattgtcaat tatatattga gctaaattta catgacccaa
     5401 atgaactgga ttgaaagaac caaaatatag accaattttt ttattcgtat tttcagtttg
     5461 agtattcatt aatttcgaaa attatttatt cagaaaatct cgaacggttt cttcaatttc
     5521 cttttttgct ttttccagat catcattaat gataatttta tcaaattggg gagcaaaact
     5581 catttcataa gcagctttct ctattctttt ctgaatcatt tcaggtgttt ctgttcctct
     5641 tttgagcaaa cgttctttta atgcctccat gttaggtgga gcaacgaaaa tagctaaagc
     5701 ctgattacca aattgtcttt ttatgttgag cccacctttt acatccacat caaaaatgac
     5761 attcttatct ttttgggtga ttctatcaat ttcacttcgc aatgttccgt aaaaacaatc
     5821 tggataaact tcctcgtatt ctaaaaattc tccattggca attttttgtt taaattcttc
     5881 aggtgtcaaa aaataataat caatgccttc tttttcattc agacgtggag cacgactggt
     5941 agcagaaaca gaaaattcaa gattcaaatt gctgtttaat aaatgatgta ccaatgtact
     6001 tttccctgc
//
LOCUS       DAOTKS010000049         6006 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_12111_length_6006_cov_4.150059, whole genome shotgun sequence.
ACCESSION   DAOTKS010000049 DAOTKS010000000
VERSION     DAOTKS010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6006
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12111_length_6006_cov_4.150059"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="P5236_01700"
     CDS             complement(<1..206)
                     /locus_tag="P5236_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRU63107.1"
                     /translation="MNLLEALNTLDTNLFLFFNGLHNSFFDTFMYIFSNKFIWVPLYL
                     TIAWAVIKYWKRDAVAVIFIMILCV"
     gene            complement(246..998)
                     /locus_tag="P5236_01705"
     CDS             complement(246..998)
                     /locus_tag="P5236_01705"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRU63108.1"
                     /translation="MKRFFLIYFFSLIFFLPLVAQSDAIKRANDFYAKGNYEAAAQEY
                     EKILKEEGFSPELYYNLGNAYYKMNEIGKAILNYERALRLSPRFEDARFNLQMAQQKL
                     VDNISESSTFFIQRWLTSLINFCTSNQWFIVSFILFVVTLICVFIFFFKREVNVRKIA
                     LSCGIVFLILTIVCLTFSGIRKNEEVNHNQAIILSGVVTVKSAPDQSGTDLFQLHEGT
                     KVTIKSSLGNWVEIKIANGSVGWLETKDMEVI"
     gene            complement(1023..2837)
                     /locus_tag="P5236_01710"
     CDS             complement(1023..2837)
                     /locus_tag="P5236_01710"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HRU63109.1"
                     /translation="MKRKILLCLVFLTGILSAKADEPVSFTASAPSTVILDKPFQLVY
                     TINAVGKDLRIPEIKDFDILAGPFESQSSSIQIINGKRTTSVSNSYTYTLLPKRTGTF
                     TIPSASIMVDNQKYTSNGLTIKVLPADKESSKSEKQESSTSPSNISEKNIFIRTSVSK
                     STVYEQEPVLLTYKLYTLLDVVQCINKKMPDFDGFLKQEIEQAQNKQFSYENYNGRNY
                     GTVVLYQALLYPQRPGVISIDNANFEAVIQVKNQTSIHSIFDDFFDSYTNVSKNLIAP
                     GVKINVKPLPQNKPSLFAGAVGTFTMSSSLSANQIKENEAVTLKINIAGSGNLKLIQA
                     PEIKFPNDFEVYDPKITNNFKTTLAGVQGTKTIEYLFIPRHHGEYTIPSAEFSYFDLR
                     DNVYKTLRTPEYTLHVLKGNESPTTTVVGNYSNKQEIKQLGKDIRYIYTGNIKLNRTS
                     QPLFGSLIGWLMYLITFLVAIVLFIIFFKKMKENENIVLVKNKRASKVAQKRLKTALK
                     FLKMGKKDEFYEEILKGIWNYLSDKLNIPIASLTRETIISELNKKEIDQSLIEQLIDI
                     LNSCEFARYAPQSGQQEMGDLYSDAIKIIEQLEDCIKQ"
     gene            complement(2905..3558)
                     /locus_tag="P5236_01715"
     CDS             complement(2905..3558)
                     /locus_tag="P5236_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRU63110.1"
                     /translation="MRNKKFILILLTFIFATSTFAQKENNDIRAGNKKYNNKKYIEAE
                     VDYRKGLLKNPNSFEANYDLGNTLFRQKKYDEASKQFEKSAALSLHDKVKKAAAYHNL
                     GNSLLLQNKIEESIEAYKNALRNNPKDNETRYNLAYAQLLLKNQKKNQDKNQNQQQQQ
                     NKDKNKENQQNKNSQPPQMTKENAQQILDALMQDEKDALKKVKEQPIKESKSSDKDW"
     gene            complement(3603..4652)
                     /locus_tag="P5236_01720"
     CDS             complement(3603..4652)
                     /locus_tag="P5236_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRU63111.1"
                     /translation="MLRFAHPSYFLLLLIIPVLIGIFLYSRIQKHRRLKTFGNPEVLS
                     PLMPNVSQRRPVLKFILQLIAIVFLVFALAEPQFGTRVENVKRQGIEVMIALDVSNSM
                     MAQDVAPNRLDKAKQILSKLIDQMTNDKIGLIVFAGDAYVQLPITVDYVSAKMFLASI
                     TPQLVPRQGTAIGSAIDLAIRSFGQKSQAGRAIIVITDGENHEDNAVEAAKLAYENGI
                     MVNVIGVGTPEGAPIPIPGTMSFKKDKEGNVVVSKLNEEMCKSIAAAGNGIYVRADNT
                     NNAYHVISKQLDNLAKTTVETKIFSNYSEQFQSFVLIALLLLIIDFFIFDRKNKKLSR
                     INIFETFTLQKRKNE"
     gene            complement(4703..5686)
                     /locus_tag="P5236_01725"
     CDS             complement(4703..5686)
                     /locus_tag="P5236_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRU63112.1"
                     /translation="MTFHNPGYLFLLLLLIPIIFWYVWEMHKSDASLQFSSLKEIKHF
                     PKSKRIKLRHLPFILRILVIVLLIFALARPQSSNSLRNKTIEGIDIVIALDISGTMLA
                     EDLKPNRLEASKAVATEFILSRPNDNIGLVVFAAQSFTQCPLTTDHDALINLIKGVNY
                     GMIEDGTAIGLGLANAVNRIKEGKAKSKIIILLTDGSNNRGDIAPITAAEIAKTFGIR
                     VYTIGVGSHGMVKVPVQTPFGLQYQMMESEFDEQTLQEIATVTGGKYFRATDNTKLRS
                     IYQEIDKMEKTKINISEYSKKNEMYPYFVMIAFILLSLEIVLRNTILNKLP"
     gene            complement(5723..>6006)
                     /locus_tag="P5236_01730"
     CDS             complement(5723..>6006)
                     /locus_tag="P5236_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63113.1"
                     /translation="IERRFQIKGLEMTSSEILDSLKTLQKEHKTAYEALYQILQLADL
                     VKFAKWIPTDEESLLSLNNAYTFVNETKIEEEKTLIDDQEKEKQEGQVS"
BASE COUNT         1924 a         1013 c          899 g         2170 t
ORIGIN      
        1 acacacaaaa tcatgatgaa aatcacagct actgcatctc gtttccagta ttttataact
       61 gcccaggcaa tagttaaata taacggtacc catataaact tgttgctgaa aatatacatg
      121 aaagtatcaa aaaatgaatt atgtaagccg ttaaaaaaaa ggaataaatt ggtatccaac
      181 gtatttaatg cctctaacaa attcattttc tggtatttta aagaaataaa aatgtacaga
      241 aaagattaaa ttacttccat gtctttagtc tccaaccatc ctacactgcc attagcaatt
      301 tttatttcta cccaattacc cagtgaactt ttaatggtga ctttggttcc ttcgtgtaat
      361 tgaaacaaat ctgttccact ctgatcagga gcacttttta ctgttactac acccgaaaga
      421 ataatagctt gattgtgatt tacttcctcg ttttttcgaa tgccagaaaa agtcaagcaa
      481 acaatggtta aaatcaaaaa aacaattcca caggataatg caatttttcg gacgttcact
      541 tctcttttaa aaaagaaaat aaaaacgcat atcagcgtga caacaaacaa aataaaactg
      601 acaataaacc attgattaga agtacaaaaa ttaattaaac ttgtcaacca acgttgaata
      661 aaaaaagtag aagattcact gatattatct accagtttct gttgagccat ttgtaagttg
      721 aaacgtgcat cttcgaatcg tggcgataaa cgtaaagccc gctcgtaatt caaaatagct
      781 ttcccaattt cattcatttt ataataagca ttccccagat tataataaag ttcaggagaa
      841 aaaccttctt cttttaaaat tttttcatat tcttgagctg ctgcttcata attccctttt
      901 gcatagaaat cattagctcg tttgatagcg tcactttgag ccacaagagg tagaaaaaaa
      961 atcaatgaaa aaaagtaaat taaaaagaaa cgtttcataa ttttaaaagt ctaatttctt
     1021 ttttattgtt taatgcaatc ttctaattgc tcaataattt taatggcgtc agagtataaa
     1081 tcacccatct cttgttgtcc cgattgaggt gcataacgtg caaattcaca agagttcaaa
     1141 atatcgatta attgttcaat aagggattga tctatttctt tcttatttaa ttctgaaata
     1201 attgtttctc tggtcagaga agcaatcggt atatttaatt tatcactcaa ataattccag
     1261 attcctttta aaatttcctc ataaaattca tcttttttac ccatctttaa aaatttgaga
     1321 gcagttttga gccttttttg tgcaactttg ctggctcttt tattttttac taacactatg
     1381 ttttcatttt ctttcatttt cttaaagaaa ataatgaaca aaacaatggc taccaaaaaa
     1441 gtgataagat acattaacca acctattaac gaaccaaata gcggctgaga agttcggtta
     1501 agtttaatgt ttcccgtata aatataccgt atatctttcc ctaattgttt aatttcctgt
     1561 ttattactat aattgccaac aactgtcgta gtgggacttt catttccttt cagtacgtgt
     1621 aaagtatatt caggcgttcg aagagtttta taaacattat ctcttaaatc aaaataagaa
     1681 aattctgctg aaggaatggt gtactctcca tgatgacgag gaataaataa atattcaatt
     1741 gtttttgtgc cttgaacacc ggcaagagtt gttttaaaat tgttagtaat ttttggatca
     1801 tacacttcaa aatcgttagg aaattttatt tcaggagctt gaattaactt taaattacca
     1861 cttccggcaa tattaatttt taaagtaact gcttcatttt cttttatttg atttgcagaa
     1921 agagaagagc tcatggtaaa ggttcccact gcaccagcaa aaagtgatgg tttgttttgg
     1981 ggcaatggtt ttacatttat ttttacacca ggggctatca gatttttaga aacattagta
     2041 taagagtcga aaaaatcatc aaaaatgcta tgaatagaag tttgattttt gacttgaatt
     2101 acagcttcaa aatttgcatt atcaatggaa atgacacctg gtcgttgagg ataaagcaaa
     2161 gcctgatata aaaccactgt gccataattt cgtccattgt aattttcata agaaaattgt
     2221 ttgttttgtg cttgctcaat ttcctgtttc aaaaaaccgt caaaatcagg cattttctta
     2281 ttaatacatt gtaccacatc cagtaaagtg tatagtttat aggttaataa tactggttcc
     2341 tgttcgtaca cagtagattt ggaaacggaa gtacgtataa aaatattttt ttcagaaatg
     2401 ttgcttggcg aagttgaact ttcttgtttc tccgatttgg atgattcctt gtcagcaggt
     2461 aaaactttta tggttagtcc attggaagta tacttctgat tatctaccat gatgcttgca
     2521 gaaggaatgg taaaagtccc cgttcgttta ggcaacaaag tataagtata tgaattactt
     2581 acgcttgtag tgcgtttccc gttaataatc tggatacttg aactctgaga ttcaaaggga
     2641 cctgccagaa tgtcgaaatc ttttatttct ggaatacgta aatctttccc tactgcattt
     2701 attgtataaa caagttgaaa tggtttatcc aaaataacag tactcggtgc agaagccgta
     2761 aaagatactg gctcatctgc ttttgcagaa agaattccag ttaaaaaaac taagcacaga
     2821 agtattttgc gtttcattgg ttacagtatc ttcaaattgt cttttaaatt aataatttgc
     2881 aataaataag ttacgaacat aattttacca gtctttatct gaagatttgc tttcttttat
     2941 aggttgttcc tttactttct ttaaagcatc tttttcgtcc tgcatcaaag catctaaaat
     3001 ttgttgtgca ttttcttttg tcatttgagg cggttgcgaa tttttgttct gttgattttc
     3061 tttgttttta tctttatttt gctgttgttg ctggttctgg tttttatcct gattcttttt
     3121 ttgatttttc agaagtaatt gggcataagc caaattgtaa cgtgtttcat tatctttggg
     3181 attatttctt aaagcatttt tatatgcttc aatactttct tcaattttgt tttgtagcaa
     3241 aagtgaattc cccaaattat gataagctgc agcttttttt actttgtcat gcaaagataa
     3301 tgcagcagat ttttcaaatt gtttacttgc ttcatcgtat tttttttgac ggaaaagtgt
     3361 gtttcctaaa tcataatttg cctcaaatga atttggattt tttagtaaac ctttccgata
     3421 gtctacttcg gcttcgatat attttttgtt gttgtatttt ttattgccag ctctaatatc
     3481 attgttttct ttttgtgcaa aggttgaagt tgcaaatata aatgttaata atatcaatat
     3541 gaattttttg ttcctcattg tttatctttg attttttaac ttataccaaa ttaaaaaaat
     3601 tattattcat ttttcctttt ttgaagagta aaagtttcaa aaatattaat tctacttaat
     3661 tttttatttt ttcgatcaaa gataaagaaa tcaataatta ataacaaaag tgctattaat
     3721 acaaatgatt gaaattgctc gctataattc gaaaaaatct tggtttccac tgttgtttta
     3781 gccagattat ccaattgttt agaaatgaca tggtaagcat tattggtatt gtcggctcga
     3841 acataaattc cgttcccggc agcggcaata cttttacaca tttcttcatt taactttgaa
     3901 accactacat taccctcttt atcttttttg aaactcattg ttcccggaat tggaataggg
     3961 gctccttctg gtgtgcctac gccaattaca tttaccatga tgccattttc ataagcaagt
     4021 ttagcagctt ctactgcatt atcttcatga ttttcgccat cggtaataac aataattgct
     4081 ctaccagcct gacttttctg accaaaagat cgtattgcca gatcaatagc tgaaccaata
     4141 gcagttccct gccgtggaac caactgaggt gtaatagaag caagaaacat tttggctgaa
     4201 acataatcta ccgtaatagg caattgtacg taagcatcgc cggcaaatac aattaaccca
     4261 attttatcat tggtcatctg gtcaataagt ttagaaagaa tttgttttgc cttatccagt
     4321 cggttaggag ctacatcttg agccatcatt gaatttgaaa cgtctaaagc aatcataact
     4381 tctattcctt gccgtttaac attttcaact cttgtaccaa attgtggttc ggctaatgca
     4441 aaaactaaaa aaacaatcgc aattaattga agtataaatt ttaaaactgg acgtctctgt
     4501 gaaacattag gcattaaagg tgacaatacc tctggattac caaaagtttt cagacgacgg
     4561 tgcttttgaa tccgactata aagaaatatt cctattaata cgggaattat cagcagcaac
     4621 agaaaataac ttggatgtgc aaatcgcaac ataaaattat tttttaatca taaaagttgg
     4681 taaccaaata aatacaaaat aattaaggta atttatttaa tattgtattt cgcaatacaa
     4741 tttctaaact cagcaaaata aaagcaatca ttacaaaata aggatacatt tcattttttt
     4801 tgctgtattc actaatgttt atttttgttt tttccatttt atcaatttcc tgataaatgc
     4861 ttcttagttt ggtgttatca gtagctctaa aatatttccc tcctgttact gttgcgattt
     4921 cctgaagtgt ttgttcatca aattcggatt ccatcatttg atattgtagt ccaaaaggcg
     4981 tctgaacagg aactttcacc ataccatgcg aaccgacacc tatggtataa actcttattc
     5041 caaaagtttt tgcaatttca gcagcagtta ttggggcaat atcacctcta ttattagagc
     5101 catcagtgag caaaataata atttttgatt tagcttttcc ttctttgatg cggttcacag
     5161 cattagcaag acctaaacca atagctgttc catcttcaat catgccataa tttactccct
     5221 taataagatt aatcaaagca tcgtgatcgg tggtcaatgg acattgtgta aaactctggg
     5281 ctgcgaatac taccaagcca atattatcat tgggacggga caaaataaat tctgtggcta
     5341 ctgcttttga agcttccagc cgattgggtt tcaaatcttc tgcgagcatt gttcctgaaa
     5401 tatctaaagc aatcacaata tcaattcctt cgattgtttt atttctcaaa ctgtttgaag
     5461 attgaggtcg agccagagca aaaataagta aaacaataac aagtattcga agaataaaag
     5521 gtaaatgtct taattttatt cttttacttt tagggaaatg ttttatctct ttcaacgaag
     5581 aaaattgtaa actcgcatcc gatttatgca tttcccacac ataccagaag ataatcggta
     5641 ttagtaacag taataaaaat agatatcctg gattatgaaa agtcattgtt tcattaataa
     5701 tttaaaaaac cgaaataagt ttctaagaaa cttgtccttc ttgtttttct ttttcctgat
     5761 cgtcaattaa agttttttct tcttctattt ttgtttcatt tacaaacgtg taagcattat
     5821 tcagacttaa caaactttct tcgtctgtag gtatccattt tgcaaatttt accagatcag
     5881 ctaattgcaa tatttgatac aaggcttcat aagctgtttt atgctctttt tgtaaggttt
     5941 tcaagctatc taaaatttca ctcgaagtca tttccagccc cttaatttga aaacgccttt
     6001 caatat
//
LOCUS       DAOTKS010000050         5957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_12219_length_5957_cov_4.341240, whole genome shotgun sequence.
ACCESSION   DAOTKS010000050 DAOTKS010000000
VERSION     DAOTKS010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5957
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12219_length_5957_cov_4.341240"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="P5236_01735"
     CDS             <1..245
                     /locus_tag="P5236_01735"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HRU63114.1"
                     /translation="LIRGLNDIIGSGKVGRELKLDRSFYGENLITSQRIWIENTNEKI
                     VFKSAPRIGINYAGEEWINKPWRFILEREKNPPSLK"
     gene            complement(292..600)
                     /locus_tag="P5236_01740"
     CDS             complement(292..600)
                     /locus_tag="P5236_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HRU63115.1"
                     /translation="MEDEKNQISIELSAEVAEGIYSNLAIISHSSSEFVIDFIRIMPG
                     IPKAPVKSRIILTPEHAKRLLFALEDNIAKYEQQNGKIKIAENTFIPPFPMGFGGGEA
                     "
     gene            972..>2038
                     /locus_tag="P5236_01745"
     CDS             972..>2038
                     /locus_tag="P5236_01745"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRU63116.1"
                     /translation="MAEKSKYIKAATPSTVQISPNEYGKMPPQAPELEEAVLGALMIE
                     KDAYFQVSEILKPEYFYKTAHQKIFEAIVTLAYHQEPIDMHTVTEQLKKNGSLEEVGG
                     PYYITILTAKVSSAAHLEYHAKIIVQKYLARELIRISNEIQSKAYDEKTDVDDLLEES
                     EAMLFEVSQRNLKKEVLPISSVLDEAIQRIQAAASREGASGIPTGFHSLDKITSGWQK
                     SDLIIIAARPSMGKTAFVLSMAKNMAMNYNIPVGIFSLEMSNIQLANRLIMNICEIDG
                     SKIKNGELEPYEWEKLDKNLKDLIDAPIYIDDTPSLSIFELRSKARRLVKEHHVQCII
                     IDYLQLMNASGMNFGSREQEV"
     assembly_gap    2039..2138
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2139..2384
                     /locus_tag="P5236_01750"
     CDS             <2139..2384
                     /locus_tag="P5236_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HRU63117.1"
                     /translation="LLGIAEIIIAKHRNGATDMIQLKFKSEFAKFLNLDDFDAQEEIL
                     NEYKVIPSKINKTEKSTASRFSSLDSFLYDNSEEVPF"
     gene            complement(2430..3680)
                     /locus_tag="P5236_01755"
     CDS             complement(2430..3680)
                     /locus_tag="P5236_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HRU63118.1"
                     /translation="MSLKFRLVILSFLQYFIWGSWLISLGSYLGNVLGFDGIQIGSIF
                     GAMGIVSLFMPGLLGIVADRWIPAQKLLGICHLLGAIFLILASFQREYFPFYILMFLN
                     VMFYMPTISLSNAVAYNALEEAHYDIVKDFPPIRVWGTIGFICAMWTVDLTGFKYSQV
                     QLYVAATAALLLGIYSFTLPPCKINKTQEKKSLVDALGLRAFSLFKQKTMAIFFIFSM
                     LLGASLQITNGFGDLFLRSFEKMPEYINSFGVKHSVILLSISQMSETLFILAIPFFLK
                     KFGIKKVMLISMLAWVFRFGLFGLGNPGERLWMLILSMIVYGMAFDFFNISGSLFVEQ
                     STDSSIRASAQGLFMIMTNGLGATMGSYLAGMVVDKFVTHAAVPSWSTAWFIFSGYAL
                     VVAILFAILFKYKYEPEKSPEISH"
     gene            complement(3798..4397)
                     /locus_tag="P5236_01760"
     CDS             complement(3798..4397)
                     /locus_tag="P5236_01760"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HRU63119.1"
                     /translation="MDSEIKLVISGLVYNQNIVGTYGLILSEESGNRRFSVMIGEPEA
                     QSIALKLNNKKLPRPLTHDLIKNILDAFEVTLVKVLISDMINDVFYSELHIKKDNKIL
                     VVDSRTSDAIALAVRCNCPIYIKSEILDMVGVVIEPTNEEEETIPEKTEYDKISDSQL
                     DRLSLEELDELLSMAVNEEKYELAVHIRDAINRKKSSKQ"
     gene            4672..5247
                     /locus_tag="P5236_01765"
     CDS             4672..5247
                     /locus_tag="P5236_01765"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008778337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRU63120.1"
                     /translation="MKFELPKLPYAYNALEPVISEKTISFHYGKHHQTYVNNTNNLIV
                     ATEFENADLETIIKKTSGALFNNAAQVWNHNFYFLALTPDKGTKPSDKLLKAIESGWG
                     SLDNFKTEFNKLAIALFGSGWTWLVKDKDGKLAIVNESNAGNPIVQGLTPILTFDVWE
                     HAYYLDYQNRRADYVAALWELINWEVVSGKY"
     gene            complement(5268..5747)
                     /gene="ispF"
                     /locus_tag="P5236_01770"
     CDS             complement(5268..5747)
                     /gene="ispF"
                     /locus_tag="P5236_01770"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRU63121.1"
                     /translation="MNFRVGFGYDVHPFKEGRELWLGGIKIDYPYGLAGHSDADALIH
                     AICDALLGAACLGNIGIHFPDTSDEFKNIDSKILLDRTLQLIRKNGYEIGNIDCTVCA
                     EEPKLNPYIPAMQKCLSKIMNIKIDDISIKATTSEKMGFVGRKEGIAVYAVTLIYKK"
BASE COUNT         2140 a          969 c          900 g         1848 t
ORIGIN      
        1 tattgattcg aggtttaaat gacataatcg gttcagggaa agtaggacgc gaactaaaac
       61 tcgacagaag tttttatgga gaaaatctca ttacctctca aagaatttgg attgaaaata
      121 ccaatgaaaa aattgtattc aaatcagctc cacgcatagg aataaactat gccggcgaag
      181 aatggataaa caagccatgg agatttatac ttgaaagaga aaaaaatcct ccctccttaa
      241 aataaaccaa aaaaataatt attccttgat accaattact attaatttca atcatgcttc
      301 tcctccacca aatcccatag gaaaaggagg aataaaagta ttttcagcaa tttttatctt
      361 tccattttgc tgttcgtatt tagcaatatt atcttctaac gcaaacaaaa gccttttggc
      421 atgctcagga gtaagaatga ttcgcgattt tacaggagct ttgggaatac ctggcataat
      481 tcgtataaaa tcaatgacaa actccgaaga ggaatgcgag ataatagcta agttagaata
      541 aataccttct gctacttcgg ctgaaagttc aatgctgatt tgattttttt cgtcttccat
      601 aaaataaata attaattaat tatcaaatga ttaactttaa aataagaatt aaagtgctaa
      661 atattttttc aaaataaaga aaaaactttt ccttgctttc cagaaatcgc tgattaattt
      721 aagttttttc ctcaaaaagt tgcaaaaata aaacagttaa ccatagagca aagatagata
      781 atttaggcaa tgaaacaaca tgattctgcc aaaaaatata gcttcttaaa attaactaca
      841 ataaacattg tcttttgcct tttcaaaaaa actttaacat ttttattttt aaaaatattt
      901 tacgaaagga gaaaaaaatt gtatttttgg aaagaagttt ataaaatcat atttatttct
      961 tattctgaca aatggctgaa aaatcaaaat atataaaggc tgcaacacct tcgacggtac
     1021 aaatttctcc caacgaatac ggaaaaatgc ctcctcaagc tccagaactg gaagaagccg
     1081 tattaggagc tttgatgatt gaaaaagatg cttattttca ggtttcagaa attttaaaac
     1141 ctgaatattt ttacaaaacc gctcatcaaa aaatttttga agccattgtt acattagctt
     1201 atcatcagga gccaatagat atgcatactg ttaccgagca gcttaaaaaa aatggttcgc
     1261 ttgaagaagt aggaggtcct tattatatta ctatacttac agctaaggtt tcttcagcag
     1321 ctcatctgga atatcatgca aagattattg ttcaaaaata tcttgctaga gagttaataa
     1381 gaatttcaaa cgagatacag tcaaaagctt atgacgaaaa aaccgatgta gatgatttat
     1441 tggaagaatc ggaggcgatg ttatttgagg tttcgcaacg taacttaaag aaagaagttc
     1501 ttccaattag ttctgtatta gacgaagcta ttcaacgtat tcaggctgct gccagtcgcg
     1561 aaggtgcaag cggcattccc acaggtttcc attcattaga taaaattact tcgggttggc
     1621 aaaaatcaga tttaatcatt attgcagcgc gaccttcgat gggaaaaacc gcatttgttc
     1681 tttcgatggc aaaaaacatg gcaatgaatt ataatatccc ggtgggaatt ttttctttag
     1741 aaatgtcgaa tattcaatta gccaatcggc ttattatgaa tatttgtgaa atagatggat
     1801 caaaaatcaa aaatggagaa ctcgagcctt atgaatggga gaaattggat aaaaatctta
     1861 aagatttaat tgatgctccc atttatattg atgatacgcc cagtttatcc atctttgaat
     1921 tgagaagtaa agcacgtcgt ctggtgaaag aacaccatgt tcaatgtatc attatcgatt
     1981 atttgcaatt aatgaatgcc agcggaatga attttggcag ccgagaacaa gaagtgagnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct tttgggaatt gctgaaatta
     2161 ttattgccaa gcatagaaat ggtgcaacag acatgattca actgaaattt aaaagtgaat
     2221 ttgcaaaatt tttaaatctg gatgattttg atgctcagga ggaaattttg aatgaataca
     2281 aagtaattcc ttcaaaaata aataaaactg aaaaaagcac tgcttctcgt ttttcctctc
     2341 ttgactcgtt tttatatgac aatagtgaag aagttccttt ctaaaaaggt tttttcattc
     2401 tcattcaaat tcaaaaacac ttttattttt taatgtgaaa tctctggtga cttttcaggc
     2461 tcgtatttgt atttgaataa tattgcaaat aaaattgcca caactaaagc ataacctgaa
     2521 aaaataaacc atgctgttga ccaacttgga acagccgcat gagtgacaaa cttatcaaca
     2581 accattccag ccaaataaga acccattgtt gcccccaaac catttgtcat aatcataaat
     2641 aatccttgag cagaagcccg aatagaagaa tcagtagatt gttctacgaa taaagatccc
     2701 gaaatgttga aaaaatcaaa agccattcca tagacgatca tggaaagaat cagcatccac
     2761 aatctctctc ctggatttcc caatccgaaa agtccaaaac gaaaaaccca ggccagcata
     2821 ctgattaaca ttactttttt tattccaaat tttttcaaga aaaaaggaat agccagaatg
     2881 aacaatgttt cggacatttg tgaaatggag agtaaaataa cagaatgctt taccccaaat
     2941 gaattgatat attcaggcat cttttcaaaa cttctgagaa acaagtcgcc aaacccattt
     3001 gtaatttgca gtgaagctcc caataacatg gaaaagataa agaaaattgc catcgttttt
     3061 tgcttaaaaa gtgaaaaagc acgcaatcct aaagcatcaa caagcgactt tttttcttgt
     3121 gttttattga ttttacaagg aggtaaagta aaagaataaa ttcccaacag taaagctgca
     3181 gttgctgcta cataaagttg aacttgtgaa tatttgaagc ccgtaagatc aaccgtccac
     3241 attgcacaaa taaaaccaat cgttccccat acacgaatag gaggaaaatc ttttactata
     3301 tcgtaatgag cttcttctaa agcgttataa gctacagcat tacttaacga aatagttggc
     3361 atataaaaca ttacattgag gaacattaaa atataaaaag gaaaatattc tctttgaaaa
     3421 gaagccagaa taagaaaaat agctcctaaa agatgacaga ttcctaataa tttttgagca
     3481 ggtatccagc ggtcggctac aattcctaac aaaccaggca taaaaagaga aacaattccc
     3541 atagccccaa aaatacttcc gatctgaatg ccatcaaatc ccaatacatt tcctaaataa
     3601 cttcccaatg aaataagcca cgaaccccag ataaaatatt gaagaaaact taatataaca
     3661 agacgaaact ttaaactcat gatgattaaa atttttattt taaatatctt gttgattcag
     3721 aaaagattaa aaatatcttt tatgaaaatt tggaaatttt ttctatttca aaattgagca
     3781 aacaaaaagg aaagctgtta ttgcttgctt gattttttac gatttatggc atctcgaata
     3841 tgcaccgcca attcatattt ttcttcattt actgccatag aaagtaattc atctagttct
     3901 tccagagaaa gtcgatctaa ttgtgaatct gatattttat catactcagt tttttcaggg
     3961 atagtttctt cttcttcatt tgttggttct attactactc ctaccatatc taaaatttca
     4021 gatttgatat atattgggca attgcatctt accgccaatg cgatggcatc agaagtacga
     4081 gaatcaacta ccagaatttt attgtccttc ttgatatgta attctgaata aaaaacatca
     4141 ttgatcatgt cgctgataag aacttttact aatgtaactt caaaagcatc taaaatattt
     4201 ttgattaaat catgtgttaa ggggcgtggt aattttttat tattcagttt taaagcaata
     4261 gattgggctt ctggctcacc tatcattacc gaaaatcttc gattgcccga ttcttcactt
     4321 aatatcaaac cataggtgcc aacaatattc tgattgtaaa ctaacccact aatcactaat
     4381 tttatttccg aatccatagt attttttaaa agaaattttt atagagataa aggtataaaa
     4441 aacttttaaa ttatatcaga acaacaaata tactataatt ttattactaa aaacagataa
     4501 ataagtgaaa attttttagc gggtaagttt tgaagtcctt ttttattaag tcacctgatt
     4561 ttttatcatc tttgatagga tttttttaag aaataaaaca aattgaattt ttacgtgtta
     4621 taaaaaatat aaaagaaaat tattaatact catcttaaaa aaattattgc tatgaaattt
     4681 gaattaccaa aattaccgta tgcttataat gcattagagc ctgtgataag tgaaaaaact
     4741 attagtttcc actatggtaa acatcatcaa acttatgtaa ataataccaa taatttaata
     4801 gtggcaacag aatttgaaaa tgctgatctg gagactatta ttaaaaaaac cagtggtgca
     4861 ctctttaaca atgctgcaca agtctggaat cataattttt attttttagc tttaactcct
     4921 gataaaggta ccaaaccctc tgataaattg ttgaaagcaa ttgaatcagg atggggttca
     4981 ctggataatt ttaagacaga atttaataaa ttagcaatag ctctttttgg ttctggttgg
     5041 acttggctgg taaaggacaa ggatggcaaa ctcgcaattg tcaatgaaag caatgcaggt
     5101 aatcctatcg tacaaggatt gacgccaatt ttaacatttg atgtttggga acacgcttat
     5161 tatcttgatt atcagaatcg tcgtgccgat tatgtggctg ctctctggga attgatcaat
     5221 tgggaagttg tttctggaaa atattaatac tatactttca tcaatattta ttttttataa
     5281 atcagtgtaa ctgcatacac tgcaatacct tcttttcgcc caacaaaacc cattttttca
     5341 gaagtagttg ctttaatgga aatatcatct attttaatat tcataatttt ggataaacat
     5401 ttttgcatgg ctggaatata aggattaagt tttggttctt ctgcacaaac ggtgcaatca
     5461 atatttccaa tttcatagcc attttttctg attaattgta atgttctatc caataagatt
     5521 ttgctgtcaa tatttttaaa ttcatcagaa gtatcaggaa aatgaattcc aatattacct
     5581 aaacaagctg cacccaacaa agcatcgcaa atagcatgaa taagtgcatc ggcatcagaa
     5641 tgacctgcca aaccataagg ataatcaatc ttaattcctc ccagccataa ttctcgtcct
     5701 tctttaaagg gatggacgtc ataaccaaaa ccaactcgaa agttcattac caatcagtta
     5761 taaaaaaaca gttgttcaac catagaattg gataatatgg gaacaactgc aaagataaat
     5821 acaaaacgca aagatctaaa tttattttat taaatttttc aatccattta tgtcaaatgc
     5881 taacgtaaat cgcaatgttt ggtccaaagg attggattga ttggtagcaa taacataacc
     5941 tgcatcaagt tcaaaca
//
LOCUS       DAOTKS010000051         5816 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_12536_length_5816_cov_4.354105, whole genome shotgun sequence.
ACCESSION   DAOTKS010000051 DAOTKS010000000
VERSION     DAOTKS010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5816
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12536_length_5816_cov_4.354105"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..750
                     /locus_tag="P5236_01775"
     CDS             148..750
                     /locus_tag="P5236_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018362542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HRU63122.1"
                     /translation="MLSTYKTIANKSEGIYKDKGSKFFSFAVPTQSVDGVKELVKNFK
                     EEFHDARHICYAYVIGIEHKEWRANDDGEPAGTAGRPILGQINSRGLTNILVVVVRYF
                     GGILLGTSGLATAYREAAADALNHAEIVEKTIDNFFTIRFNYSKINEVIRLLKEFNGE
                     IFNKNYDKNEYIIEFSIPKKDAALLISKLKNITGLTIEQQ"
     gene            1090..1995
                     /gene="pyrB"
                     /locus_tag="P5236_01780"
     CDS             1090..1995
                     /gene="pyrB"
                     /locus_tag="P5236_01780"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HRU63123.1"
                     /translation="MSIKSLVSITDYSKEDILEILSSAEEFEKHPNQEVLKGKVIATL
                     FFEPSTRTRLSFETAVVRLGGSVIGFSDVATSSLSKGESLHDTIRIVSNYADAIVMRH
                     PYEGAARYAAEISPVPIINAGDGANQHPSQTLLDLYSILKTQGTLENLNICIVGDLKY
                     GRTVHSLIMAMSHFHPTFTFIAPEELRIPYEFKIFCDKNNISYTELYELSDNFNEADI
                     LYMTRVQKERFQDLMEYERVKNVYMLKNSMLENTKPNLRILHPLPRVFEIDPEVDKNK
                     KAYYFQQAKNGLYVRQAIINKVLSS"
     gene            2084..2539
                     /gene="pyrI"
                     /locus_tag="P5236_01785"
     CDS             2084..2539
                     /gene="pyrI"
                     /locus_tag="P5236_01785"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HRU63124.1"
                     /translation="MEKELKVAALRNGTVIDHIPVDKLYKVVFMLHLDRCENQITIGN
                     NLDSVKMGKKGIIKIAGKFFAEDETNKIALIAPNANINIIKDAQVIEKRALSLPNEIH
                     DIIQCNNPNCITNHQNVPTFFYVLKEAEGTFLKCHYCERIISQEEVRIK"
     gene            2636..3181
                     /locus_tag="P5236_01790"
     CDS             2636..3181
                     /locus_tag="P5236_01790"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRU63125.1"
                     /translation="MVYPLPAALISCGNSPDEYNLMTVSWVGTICSNPPMCYISVRPE
                     RHSHSIIKKNKEFVINLTNEELAYATDWCGVKSGKDYNKFKEMNLTPLKGEKVNAPIV
                     EESPLNIECKVKEIISLGSHDMFISEVVNIQVDNKYFDLKTHSFHLEKAKLIANLNSH
                     YYKLGEEIGKIGWTIKEKDHK"
     gene            3277..4557
                     /gene="glyA"
                     /locus_tag="P5236_01795"
     CDS             3277..4557
                     /gene="glyA"
                     /locus_tag="P5236_01795"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRU63126.1"
                     /translation="MKRDQIIFDIIEQEKQRQLKGIELIASENFVSDQVLQAMGSVLT
                     NKYAEGYPGKRYYGGCEIIDESEQLAIERLKNLFDAEWVNVQPHSGSQANQAVYFACL
                     KPGDKILGLNLSHGGHLTHGSPVNISGILYQPLEYNVNAETGRVDYDQMEEVALRERP
                     KLIIGGASAYSREWDYKRMREIADKIGALLMIDMAHPAGLIATGLLQNPLKWAHIVTS
                     TTHKTLRGPRGGIILMGKDFPNPWGKTTPKGEIKMMSSLLDSAVFPGVQGGPLEHVIA
                     AKAVAFEEALQPEFKQYQLQVKKNAETLANELISRGFSIVSGGTDNHCMLVDLRSKYP
                     DLTGKIAENTLVEADITVNKNMVPFDSRTPFQTSGIRLGTPAITTRGAKEDLMVEIAE
                     MIETVLDNIDNKKIISSVKEHVNKTMSNYPLFAY"
     gene            4638..>5816
                     /locus_tag="P5236_01800"
     CDS             4638..>5816
                     /locus_tag="P5236_01800"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HRU63127.1"
                     /translation="MNVKGKIAVIGGGSWATAIAKIVLTNEDTINWYMRRQEQIDEFI
                     RLSKNPSYLTGVKFDTERINFTNDINKVVRSSDVLIFATPSPFLKQHLKKLRVKLNKK
                     FIVSAIKGIVPDENLTVTEYFQQFYGILPENLAVLAGPCHAEEIALERLSYLTIACTS
                     REKARILANRLATNFVKTSISDDVIGIEYASVMKNIYAVAAGICHGLKYGDNFQAVLL
                     SNAIHEMSRFCNAMNPIHRDINESAYLGDLLVTAYSKFSRNRLFGTMIGKGYSVKTAQ
                     MEMEMIAEGYYAVKCIHEINENYQINMPIVDAVYQNLEIAAQRGNILSQLGLRPGDFF
                     LVTVHRQENVDDRTRFSSIIAGLEALSQRYQLPVIYPIHPRSRKMLQQFGLQPCGITL
                     L"
BASE COUNT         1999 a          855 c         1089 g         1873 t
ORIGIN      
        1 tactgaaaat actttaaagg agaagtatac tgcttaaaaa ataatctact atttttctat
       61 caaagaaaat tagacgaaat aaaataaata aatatctttg cagcgaagtt ctcatagtta
      121 gaagtattaa aaaattcaat tcgcaaaatg ctaagtacct ataaaactat tgctaataaa
      181 tcagaaggaa tttataagga taaagggagc aaattctttt cctttgcagt tcccactcaa
      241 agcgtggatg gagttaagga actggtaaaa aatttcaagg aagaatttca tgatgctcgt
      301 cacatttgtt atgcttatgt gattggtatt gaacataaag aatggagagc caatgatgat
      361 ggagaacctg ctggaactgc tggacgacct attttagggc agatcaattc acgaggattg
      421 actaatatat tagtagtggt ggttcgttat tttggtggga tattattggg aacaagtggc
      481 ttggcaactg catatcggga agcggctgct gatgccttga atcatgctga aattgttgaa
      541 aaaaccattg ataatttctt taccattcgg tttaattatt ctaaaataaa tgaagtgata
      601 cgacttttaa aagaatttaa tggggaaatt ttcaataaaa attacgataa aaatgaatat
      661 ataattgaat tttccattcc taaaaaagat gcggctcttt taatttcaaa actgaagaat
      721 attacaggat taaccattga acagcaataa attttcaata tcgattaata aaagattttg
      781 agttatttta taaatttttg ttcgggtttt taaaacaaaa aaacagtttt tttaaataaa
      841 aatcaatata tttgcaaaac aaatatttct tttttgagaa gatttattga tcaatataag
      901 ggaatgaaaa gaacaaattt tcaatatttt tttatttttt gtagcttgtt aagaaaatta
      961 gcagttaaaa ttttgaaaac caatatataa ttcgtacaag atatatattg gtttttttat
     1021 tttcatgttt taaatgtttc tatagtaagg tttgagaatt ggtaaatatt aaaaaatcga
     1081 cttgcaataa tgagcattaa aagtttagtt tccataaccg attatagcaa ggaagacatt
     1141 ctggaaatcc tatcctcggc agaagagttt gagaaacatc ccaatcaaga agttttgaaa
     1201 gggaaagtaa ttgccacttt gttttttgaa ccttccacca ggacaagact tagttttgaa
     1261 acagcagtag tacggttggg aggttctgtt atcggctttt ctgatgtggc aaccagtagt
     1321 ctatcaaaag gtgagtcatt acatgatact attcgtattg taagcaatta cgccgatgca
     1381 attgtgatgc gtcatcctta tgagggtgcg gcacgttatg ctgcagaaat ttctccagtt
     1441 ccaatcatca atgctggtga tggagccaat caacatcctt cacaaacatt gctcgacctg
     1501 tattcaattt taaaaacaca aggaacacta gaaaatttaa atatttgtat agtaggcgat
     1561 ttaaaatatg gaagaacagt tcattcgctg ataatggcaa tgtcgcattt tcatccaaca
     1621 tttactttta ttgcaccaga agaattgcga ataccttatg aatttaagat tttttgtgat
     1681 aaaaataata tttcttatac cgaattatat gaattaagcg ataattttaa tgaagcagac
     1741 attttataca tgacgcgagt tcaaaaagaa cgttttcagg atttaatgga atatgagcgg
     1801 gtaaaaaatg tatatatgtt gaaaaattca atgcttgaga atacaaaacc aaatttacgc
     1861 attcttcatc cattgcctcg cgtgtttgaa attgatccag aggtagataa aaataaaaaa
     1921 gcttattatt ttcagcaagc aaaaaatggt ttgtatgttc ggcaggctat tattaataaa
     1981 gtgctttctt cttgataatt aattgattac aattttaata taaaattaga aaaatttttt
     2041 atctctcttc tcaagtatat attgtttact ttgattatat gttatggaaa aagagttaaa
     2101 agttgcagct ttgcgaaatg gtaccgttat agatcatata ccggtggata aattgtataa
     2161 agtggttttt atgttacacc tggataggtg tgaaaatcaa atcactattg gcaacaatct
     2221 cgatagtgtt aaaatgggga aaaaggggat tatcaaaatt gccggaaaat tttttgctga
     2281 ggatgagacc aataaaattg ctctgatagc gccgaatgcc aatattaata ttattaaaga
     2341 tgcccaagta attgaaaaaa gagctctaag cttaccaaat gaaattcatg atattatcca
     2401 gtgcaataat cctaattgta tcaccaatca tcaaaatgta cctacctttt tttatgtttt
     2461 gaaagaagca gaaggcactt ttcttaaatg tcactattgt gaaaggataa tttctcaaga
     2521 agaagtcaga ataaaataaa ttttgaaaaa cgagaaatgt ttaaattgct aatttttaat
     2581 taatttaatt atttagttga atcatatgtc aaaaataagt tggaaacctg tcaacatggt
     2641 atacccttta ccagcggctc ttatttcgtg tgggaatagc cctgacgaat ataatttaat
     2701 gacagtaagc tgggtaggaa ctatttgttc aaatccgccg atgtgttata tttccgttcg
     2761 tcctgaacga cactcccatt caatcataaa aaaaaataaa gaatttgtta ttaaccttac
     2821 taatgaagag cttgcttatg ctactgattg gtgcggtgta aaaagtggaa aggattataa
     2881 taaatttaaa gagatgaacc ttactccttt gaaaggtgaa aaagtaaatg ctcccattgt
     2941 tgaagaatcg ccattaaaca ttgaatgcaa ggtaaaagaa attatttctt taggttctca
     3001 tgatatgttt atttccgaag tagttaatat tcaggtcgat aataaatatt ttgatttaaa
     3061 aactcattct tttcatttgg aaaaggcaaa attaattgcc aatctaaata gtcattacta
     3121 taaattagga gaagaaattg gaaaaattgg ttggacaatc aaagaaaagg accataaata
     3181 attatttatc aaaaaattaa aaaatattta aataaaagag caattctatt ttgtaaataa
     3241 attgattagt ttttaaatca ttaatataag agaaaaatga aaagagacca aataattttt
     3301 gatattattg agcaagaaaa acaacgacag ttaaaaggaa ttgaattgat tgcttctgaa
     3361 aattttgtga gcgaccaggt attacaagca atgggttctg ttttgactaa taagtatgcg
     3421 gaaggttatc cgggcaaacg atattatggc ggttgcgaaa taatagatga gagtgaacaa
     3481 cttgctattg aacgtttaaa gaaccttttt gatgcagaat gggtaaatgt gcagcctcat
     3541 tcaggttcac aagccaatca ggcagtttat tttgcctgtt tgaaaccagg tgataaaatt
     3601 ttgggattga atttgtcaca cggcggacat ttgacgcatg gttccccagt aaatatttcg
     3661 gggattttat atcaaccact tgagtacaat gtgaatgcag aaacagggag agttgattat
     3721 gaccagatgg aagaagttgc tttgcgtgaa cgcccaaaat taattattgg tggtgcttcc
     3781 gcttattcgc gtgaatggga ttataaacga atgagagaaa ttgcagataa aattggagct
     3841 ttgttaatga tagatatggc tcatccagcg ggcttaattg caactggttt acttcaaaat
     3901 cctttaaaat gggcacacat cgtcacttct actactcata aaactttgag aggtcctcgt
     3961 ggaggaatta ttttgatggg taaagatttt cccaatcctt ggggcaagac tacaccaaaa
     4021 ggcgagataa aaatgatgtc ttctttgtta gattcggctg tttttcccgg tgttcagggt
     4081 ggtccactgg aacatgttat tgctgcgaaa gccgttgctt ttgaagaagc tcttcaacct
     4141 gaattcaaac aatatcaact tcaagtaaaa aagaatgcgg aaacattggc taatgaactt
     4201 atatcacgag gattttctat cgtttctggt ggaactgata atcattgtat gctggtagat
     4261 ttacgctcaa aatatcctga tttaactggg aaaatagcag aaaacactct ggtagaagct
     4321 gacattaccg tcaataaaaa tatggtgcct tttgattccc gtactccttt tcagacttca
     4381 ggaattcgac tgggaacccc agctattact actcgtggag ctaaggaaga tttaatggtt
     4441 gaaattgccg aaatgataga aacagtattg gataatattg acaataagaa aattatttcg
     4501 tcggtaaaag aacatgtcaa taaaactatg agcaattatc cgttgtttgc ttattagaaa
     4561 ataatttaag tccttttttc acaaagaaaa ggcaatgatt tggtgagatg aaaattaata
     4621 tttagcatga ttcaacgatg aatgtaaaag ggaaaattgc agtaattggt ggcggaagct
     4681 gggcaactgc cattgcaaaa attgttctta caaatgaaga cacaattaat tggtacatgc
     4741 gtcgtcaaga acaaattgat gaatttattc gtttgagcaa aaatccatct tatctcacag
     4801 gggttaagtt tgatacagaa cgaattaatt ttacgaatga tattaacaaa gtggtgcgtt
     4861 cttctgatgt gctcattttt gccacacctt cacctttctt aaaacaacat ttaaaaaaat
     4921 taagggttaa attaaataaa aaatttattg tttcagctat taaaggaatt gtacccgatg
     4981 aaaatctaac tgttacagaa tattttcagc aattttatgg aattttacct gaaaatttag
     5041 ctgtattagc aggtccatgt catgctgaag aaattgctct tgaaagatta tcttatttga
     5101 ctattgcttg tacttctcgt gaaaaagcca gaatacttgc caatcgtttg gctacaaatt
     5161 ttgttaaaac ttctattagt gatgatgtaa taggtattga atatgcttcg gttatgaaaa
     5221 atatttatgc agtagccgca ggtatatgtc atggattaaa atacggtgat aattttcaag
     5281 cggtgttact ttcaaatgcc attcatgaaa tgagtcgttt ctgcaatgct atgaatccta
     5341 ttcatcgcga tattaatgaa tcggcttatc ttggtgattt actggtaacg gcttattcca
     5401 agtttagcag aaatcgctta tttggtacta tgattgggaa gggatattct gtgaaaactg
     5461 ctcaaatgga aatggaaatg attgcagagg gttactatgc cgtgaaatgt attcacgaaa
     5521 tcaatgaaaa ttatcagatc aatatgccta ttgtggatgc ggtttaccag aacctggaga
     5581 tcgccgctca gcgcggcaac atcttgtctc agctcggtct tcgcccgggt gacttttttc
     5641 tggtcaccgt ccatcggcag gaaaacgtgg acgaccgtac ccggttctcc tctatcatcg
     5701 ctgggctgga agccctttcg cagcgctatc aattaccggt catctatccc attcatccgc
     5761 gctcgcgcaa aatgcttcag cagttcggcc ttcagccctg tggaattacc ctgctg
//
LOCUS       DAOTKS010000052         5781 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_12631_length_5781_cov_3.871114, whole genome shotgun sequence.
ACCESSION   DAOTKS010000052 DAOTKS010000000
VERSION     DAOTKS010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12631_length_5781_cov_3.871114"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="P5236_01805"
     CDS             complement(<1..295)
                     /locus_tag="P5236_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63128.1"
                     /translation="MKNYFYKLFFTTVCICSFLFSCTNNVDLSNISTDVQWDGAIAIP
                     IGEANLNIENLLDKIDKNNVLTTEGEEIIYQNEDSTAFRFRDIDLAKNAQSLST"
     gene            complement(515..1930)
                     /locus_tag="P5236_01810"
     CDS             complement(515..1930)
                     /locus_tag="P5236_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRU63129.1"
                     /translation="MLANYIISEIKAQLPFEPNEQQEALIETLSNFLLSKDKKKIFLL
                     KGYTGTGKTSIISAMVKAMDELYQKNILLAPTGRATKVIANYAGMPAFTIHKKIYRQK
                     SLADFHFQLSENLHSNTLFIVDEASMISETSSDKIFGSGHLFEDLLEYVYEGNNCSLL
                     LLGDTAQLPPVKEEKSPALDTERLKSYGLNVTEFTLTQVMRQALESGILYNATKLRNH
                     LSKNKVRDFPYLEIKNFKDIVSLPVSEFVNEIQKAYEEVGFDETIVITRSNYRANYYN
                     QGIRNNVLQREEEITNGDLLMVTRNNYFWSQPYEDIDFIANGDILKISRIYHYKEIYG
                     FRFADVTLFSVDENREIDVKLWLDSLQTKNPAQTEEMYKLLMERVAEDYPEIKKRNEK
                     MKRIFENEYYNALQVKFAYAITCHKSQGGQWKRVFIDPGLITEVQINADFYCWLYTAL
                     TRATEKVFLINFSENFVKKNP"
     gene            2094..2678
                     /locus_tag="P5236_01815"
     CDS             2094..2678
                     /locus_tag="P5236_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HRU63130.1"
                     /translation="MLSHLENYQIILASKSKRRQDLLSGLNIPFGVYPIEVDESFPPE
                     LIGMDIPIYLSKKKFQACTSIMNEKTMIITADTIVWYQGQVFGKPRDREEAKSMLITL
                     SGKTHDVITGVTIATFRKQRTFGVASEVRFARISEEEIDYYLDHYDPYDKAGGYAVQE
                     WIGYVGVEHINGSYYNVMGLPVQRLYNELKNWKE"
     gene            complement(2706..3971)
                     /locus_tag="P5236_01820"
                     /pseudo
     CDS             complement(2706..3971)
                     /locus_tag="P5236_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
     gene            4334..5095
                     /locus_tag="P5236_01825"
     CDS             4334..5095
                     /locus_tag="P5236_01825"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HRU63131.1"
                     /translation="MLIKKKVLILLMICMLTSCNYKTWQKVAATSDKISIDSTTDMLI
                     DKEYEAFLQPIKQKIDAEMNRVIGETLVDLKSYAPESPLSNFCADVFRQIASEFLNEK
                     IEIGIVNLGGLRASIPTGKITVRQIFELMPFENELVILWLKGDKLEKLLQYFASIGGE
                     GISGIQMTIKNGKAESILVDNQPLDPEKTYIIATSDYLAEGNDNMIQLAEYEKRIDTG
                     LKIRDILINHIQAETQKGNKIQSKTDGRITIIQAF"
     gene            5106..>5781
                     /locus_tag="P5236_01830"
     CDS             5106..>5781
                     /locus_tag="P5236_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HRU63132.1"
                     /translation="MKTKYLSILLLGLFFSFSVTFSAEKVKLVILHTNDTHSQIEPTE
                     KSPLPTSNRGGYARRMGVINQIREQEKNVLLLDAGDFWQGTPYFNFYHGRIEVDGMNR
                     MNYDAVTIGNHEFDNGLDTMAIVLKQAKFPIISSNYQFNNPLLDSIVKPYLILNKNGI
                     RIGIMALNIDPKGLILKINYKKLKFNDPLETAINVSDFLKNKKKCDVIICLSHLGCDT
                     IKQKMND"
BASE COUNT         2049 a          906 c          911 g         1915 t
ORIGIN      
        1 aagtacttaa agattgggca ttttttgcca aatcaatatc cctgaatcgg aaagctgtgc
       61 tatcctcgtt ttgataaata atttcttctc cttccgtagt gagaacatta tttttatcaa
      121 ttttatccag taaattttct atattcaagt tagcttctcc aattggaatt gctatcgccc
      181 catcccattg tacatcagta gaaatattag ataaatctac attgttggta caagaaaaca
      241 aaaaggaaca gatacaaaca gttgtgaaaa ataatttgta gaagtagttt ttcatgatgt
      301 ctggtttttg agaaaattaa aaatttaaga ataatggttg atgaataatt taaatccaaa
      361 aagagagaac ggaacttaaa aatttttaat tttctttgtt tttaaataaa aataagcaaa
      421 aaaaattaaa aagaataatg gaaatcaata ttaaattaat tgtgtgctaa aatcagcttc
      481 aaatgtaata attattttgt taaaaaaaat atttttatgg attttttttg acaaaatttt
      541 cagaaaaatt aataagaaaa actttttctg ttgctctggt taaagcagtg taaagccagc
      601 aataaaaatc agcgtttatt tgaacttctg taataagtcc cggatcaata aaaacccttt
      661 tccattgacc tccttgtgac ttatgacagg tgatggcata agcaaattta acctgtaaag
      721 cattgtaata ttcattttca aaaattcgtt tcattttctc gtttcgtttt tttatttccg
      781 gataatcttc agcaactctt tccattaaga gtttgtacat ttcttctgtt tgagcagggt
      841 ttttggtttg taaactatcc agccataatt ttacatctat ttctcgattt tcatcaacag
      901 aaaataacgt aacatcagca aaacgaaagc catatatttc tttgtaatga taaattctac
      961 tgattttcaa tatatcacca ttggcaataa aatcaatatc ttcataaggt tgtgaccaga
     1021 agtaattatt tcgcgttacc attagcaaat cgccgtttgt aatttcttct tctctttgta
     1081 aaacattgtt tctgatgcct tgattgtagt aattggctct gtaattggat cgtgttatga
     1141 caatagtttc atcaaatcca acttcttcat aggctttttg aatttcattg acaaactctg
     1201 atacaggaag cgaaacaata tctttaaaat tttttatttc cagatatggg aaatctctta
     1261 ctttattttt actcaaatga tttctcaatt ttgtggcatt gtataatatt ccactttcca
     1321 gagcttgacg cattacctgt gtgagcgtaa attccgtgac attcaaacca taacttttca
     1381 gtctttctgt atctaaagca ggactttttt cttcttttac aggtggaagt tgagccgtat
     1441 cacccagcag taataaactg caattgtttc cttcataaac atattccagt aaatcttcaa
     1501 ataaatgtcc agaaccaaaa attttatcac tggaagtttc agaaatcatc gaagcttcat
     1561 ctacgataaa aagggtattt gaatggagat tttcagaaag ttgaaaatga aaatcagcaa
     1621 gtgatttttg tcgataaatt tttttgtgaa tcgtaaaagc tggcatacca gcataattgg
     1681 caatcacttt ggtagctctt ccagttggtg ccagcaaaat gtttttttga taaagctcat
     1741 ccatggcttt taccatagcc gaaataatag aagtttttcc cgttcctgta tatcctttta
     1801 ataagaaaat ttttttctta tcctttgaaa gcaggaaatt acttagtgtc tcaataagtg
     1861 cttcttgctg ctcgtttggc tcaaaaggta attgagcttt aatttctgat ataatgtaat
     1921 tagctaacat atttacaaat atatcaagga tattttacat tttatacgaa aaatttctat
     1981 ttttgtaaaa ataaacactt tattcaagtg gaattgttta agggtttgtt ttttcattct
     2041 ttagtctgaa aaatatttat ttgcaaagtg ttttaataaa aaactaagtt atgttgcttt
     2101 cacatcttga aaattaccaa attattttag cttcaaaatc gaaaagacgt caagatttat
     2161 taagtggact gaatatacct tttggtgtat atcctattga ggttgatgaa agttttccac
     2221 cagaattaat tggaatggat attcctattt atttatctaa aaagaaattc caggcttgta
     2281 cttctattat gaatgaaaaa accatgatta ttactgctga tactattgtc tggtatcaag
     2341 gtcaggtatt tggcaaacca agagatagag aagaagcaaa aagtatgctt ataaccctat
     2401 cagggaaaac tcatgatgta attacaggtg tcaccattgc tacttttcgt aaacaacgaa
     2461 cttttggtgt tgcttccgaa gtaagatttg cccgaatctc ggaagaagaa attgattatt
     2521 atctcgatca ttatgatcct tatgacaaag ctgggggtta tgctgttcag gaatggattg
     2581 gttatgtagg agttgagcat atcaatggct cgtattacaa tgtaatggga cttcctgttc
     2641 aacggctgta taatgaattg aaaaactgga aagaatgaaa attttcccga gaaagtgatt
     2701 ttttctcatt taaagaagct gataaacagc agaacattca agattgttac tattattcct
     2761 aataaataaa gaatgatttt atgaaataag tcgtttttgt atttccccat tactttttca
     2821 gaggaagtaa gataaacttg cgaaaaaacg gtaaagggga gctggatact taaaatcatt
     2881 tgagatataa ttagtccttt aaaaggatca ccaatcaaaa agatgataat cattgccagt
     2941 atgagagaaa aagcaactcc caaacgagaa tgattatctt taatgtcata aggttcttga
     3001 tacattcctg aaaaaatagt accgccagcc attcctgatg tgatgctgga tgaaatgcca
     3061 gaaaatagta aagcaactgc aaaaaccact gctgcattat ttcctaaaag cggtgttaac
     3121 aattgatgag tttgctgtaa ttcagtaaca ggagttttag tttgataaaa agtgctagca
     3181 gcgagtaata taatagcact gttaatagcc catccgatca acatagaaac aagcgtgtct
     3241 aaaaattcat attttaattg tcttttgata acattttcat tttcaagatt ccattgacga
     3301 ctctgaatta tttcagaatg taaaaataag ttgtgcggca tcaccacagc tcccaataca
     3361 ctcataataa ttattattga accttctgga aatgaaggcg ttacacaacc ttttgctgcc
     3421 tgtgaccaat caacttttac taacgacaat tcacacaaaa aagataatcc aataatggac
     3481 acgaaagcaa ttatccattt ttcaatggct ttataagagt tggtaaaaag cataatgaga
     3541 acaaaaactg ttactaaaat tgtaccagct ggaattaaaa tatgaaacaa catgttcaaa
     3601 gcaatacttc cgcctaaaat ttcagcaagg gaagtagaaa cggaagcaca catggcagta
     3661 atgagcaaaa actttgaata aacgggcttc atatattttg aaatggcttc cgcaagacaa
     3721 agacctgtta ctattcacaa gatgtgcaac attgtgccga aaaaaattag cataatggtg
     3781 gaaaaagtaa tcatccaaat caaggcataa ccatattgtg ctccagcagc taaattcgaa
     3841 gcccagtttc ctggatcaat aaagccaacc gttaccaaca agccgggacc taaatattta
     3901 agaatctcta atcctccata aatcggacga taattttttc tatcaatatt tttaaaaatt
     3961 ttaaataaca ttgactaatt ctaaattaat tatgtaagct ttaaaaaagt aaatctcttt
     4021 ataagaactg ttttaaaaat gagtcttctt ttaaagcctg atttttcaaa agtaagaaaa
     4081 aattagcttt tttaaaggaa agaattaaat tttttgttgt ttcaattatt ttacaatatt
     4141 tgcattaaaa aaatttacta aaaattagct cttgtaaagc cttaagcgtt tttttaaaga
     4201 atatttttag tatctgaatt tgtctcggca cgagaacatt gagtagtgtt atgataactt
     4261 tttaaaaatt ctaaagttat acctaatttt ttgtattttt atccccatca ataattcaaa
     4321 aaaattaatt tttatgctta tcaaaaagaa agttttaata ctactgatga tttgcatgtt
     4381 aacttcttgc aactacaaaa catggcaaaa ggtagcagcc acatctgata aaatttctat
     4441 tgacagtaca actgacatgt tgattgataa agaatatgag gcattccttc agcctatcaa
     4501 acaaaaaatc gatgcagaaa tgaatcgcgt catcggagaa acattggtcg atttgaaaag
     4561 ttatgctcct gaaagccctc tttcaaactt ttgtgcagat gtatttcgtc aaatagcttc
     4621 agaatttcta aatgaaaaaa ttgagattgg cattgttaat ttgggtggtt taagagccag
     4681 cataccaaca ggaaaaatta ccgtacgaca aattttcgaa ttgatgcctt ttgaaaatga
     4741 actggttatt ctctggctta aaggcgataa attggaaaaa ttactgcaat attttgcttc
     4801 tatcggagga gaaggaattt caggcataca aatgaccata aaaaatggaa aagcagaaag
     4861 tattcttgtt gacaatcaac cacttgatcc tgaaaaaacg tatattatcg ccaccagtga
     4921 ttatttagcc gaaggaaatg acaatatgat tcaacttgct gaatacgaaa aacgtattga
     4981 tacaggatta aaaatcagag atatactcat caatcatatc caagctgaaa cccaaaaagg
     5041 gaataaaatt caatcaaaaa cagatggtag aatcactata attcaagctt tttaatcaat
     5101 aaaaaatgaa aaccaaatat ttatccattt tattactcgg tttatttttt tctttttctg
     5161 taacattttc agcagaaaaa gtaaaactgg ttattcttca taccaatgat acgcatagcc
     5221 agattgaacc cactgaaaaa tcaccgctgc caacttccaa cagaggtggt tatgcacgca
     5281 gaatgggcgt tatcaatcaa atacgcgaac aagaaaaaaa tgttttatta ttggacgctg
     5341 gcgatttttg gcagggaact ccctatttta atttttatca cggcagaatt gaagttgatg
     5401 gaatgaacag aatgaattac gatgcagtaa cgataggcaa ccacgaattt gacaatgggc
     5461 ttgatactat ggcaattgtg ttgaaacaag caaaatttcc catcatcagt tctaattatc
     5521 aatttaataa tccgttgctg gacagcattg ttaaacctta cttgatacta aataaaaacg
     5581 gtattcgtat cggtatcatg gctctgaata ttgaccccaa aggattgatt ttaaaaatta
     5641 attataaaaa attgaaattc aacgacccgc ttgagacagc tataaatgtt tccgactttt
     5701 tgaaaaacaa gaaaaaatgt gatgtaatta tttgcctttc acatctgggt tgtgatacca
     5761 ttaagcaaaa aatgaacgat t
//
LOCUS       DAOTKS010000053         5741 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_12712_length_5741_cov_4.535526, whole genome shotgun sequence.
ACCESSION   DAOTKS010000053 DAOTKS010000000
VERSION     DAOTKS010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5741
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12712_length_5741_cov_4.535526"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..690
                     /locus_tag="P5236_01835"
     CDS             58..690
                     /locus_tag="P5236_01835"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRU63133.1"
                     /translation="MKKVKFLLASLVIIAASSLNAQGFGVQAGYSNSTTFIGEGDTLS
                     NLNGFHVGPIAELTIQGPFSLQYGLFYNYQTKQETYEGVVTAKTSTTFHSVDIPVHLA
                     FTIPTGSPVGVFVFGGPSFNVGISKKTTSKVTSSLGTFEPPTIDYYKTDDNGDSRQSR
                     FDIQMGVGAGIKLQNLQLRLSYDWGLLDTNNSDNYSLTRDELKASIAFVL"
     gene            941..2095
                     /gene="holB"
                     /locus_tag="P5236_01840"
     CDS             941..2095
                     /gene="holB"
                     /locus_tag="P5236_01840"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HRU63134.1"
                     /translation="MLFSQIIGQKKIKERLLQSVNEQRIPHAQMLCGPEGIGKFALAL
                     AYAQYICCENKTETDACGVCPSCIKYQKLTHPDLHLVFPIIKQEKKKLVVCDDYVAQF
                     RKMVLDNPYFSENEWYAKIGSENKQGMIYTSESEEIIRKLNLKTYEAEYKVMIIWLPE
                     RMNLETANKLLKILEEPPEKTVFLLVSNSPDKIIPTILSRTQQIQIPRLNKEEIIEGL
                     IKNEHIAEEEAFNVAQISEGSYLKALSYLNEDDINKQNFEHFVKIMRLAWKLGNKKDY
                     AALKSLKNWAEEMATTSNGGRERQKNFLTYAQRMIRENFIFNLNEPALNYMTSFEKDF
                     SSGFSPFINERNVEELMNEFALAENHIERNVNPKMVFFDLLMKVVMLLKK"
     gene            2167..3366
                     /gene="ricT"
                     /locus_tag="P5236_01845"
     CDS             2167..3366
                     /gene="ricT"
                     /locus_tag="P5236_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HRU63135.1"
                     /translation="MKYKLDIGGYNINKKGCHRTSALKLSAIDWMCDLPESVKETDFV
                     EVQFKNTRKGYYLNSNKIPLEKGDMVAVEASPGHDIGEVTLVGKLVLLQMKKNKIDPE
                     KIEIKRIYRKAKETDLEKYKEAKNKEHSTMIKARQIAEKMNLKMKIGDVEYQGDGSKA
                     IFYYIADERVDFRELIKVFAETFHVRIEMKQIGARQEAGRIGGIGPCGRELCCASWMT
                     NFNSVVTSSARCQDLSLNPQKLAGQCCKLKCCMNYELDSYQDAIKDFPSKEIQLETLD
                     NTYFHFKTDVFKGLISYSTAQNMGTNLITISVQRAKEVIALNKQGIKPDKLEDQVSEE
                     IAQQEQNTENYVCQDNITRFDEKRNYHQHKNKGKNKKKQANSTNQSLKLRKNAENNRT
                     NENKEDK"
     gene            3344..3841
                     /locus_tag="P5236_01850"
     CDS             3344..3841
                     /locus_tag="P5236_01850"
                     /inference="COORDINATES: protein motif:HMM:NF025474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HRU63136.1"
                     /translation="MKIKKISKLIIFFFVGIIGTACVHHETFLEYKTLPPQGWNKDSV
                     LTFQVNITDTSSLYNVYINIRNRGEYPYQNLWLFLNIQPPHSTLPIKDTIECYLADIH
                     GKWLGSGVGNVFEMPVLYKQNECFKQSGTYTFQIIQGMRNEVLPGISNVGLKVEKISS
                     PKKHN"
     gene            3843..4586
                     /gene="trmB"
                     /locus_tag="P5236_01855"
     CDS             3843..4586
                     /gene="trmB"
                     /locus_tag="P5236_01855"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005679168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HRU63137.1"
                     /translation="MSRNKLSKFADMNSFPHVFQFPAYPFYRSDTEFNLKGKWNKDFF
                     QNDFPIILELGCGKGEYTIGLAELYPEKNIIGVDIKGARMWTGAKYSFEKQMKNVAFL
                     RTNIEAVYYFFDENEVSEIWLPFPDPQMKKVRKRLTSTHFMKIYQQFVKPGGLIHLKT
                     DSNFMFTYTCEMVKINHYPVRCITDNVYNSDNNDPALKIQTFYESQWIERGLKIKYLQ
                     FNLEKRETFFEPDIEIERDTYRSFGRNKN"
     gene            4633..>5741
                     /locus_tag="P5236_01860"
     CDS             4633..>5741
                     /locus_tag="P5236_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRU63138.1"
                     /translation="MEIYPKLILDALSHVKYPGKGKDIVSLEMVQDNIRIEGNKVSFS
                     LIFDKPNDPFIKSVVKAAEQAILTYVSPEVDIKGNITPITKEVPKPAPVLPEVENIIA
                     IFSGKGGVGKSTIAANLAAALASLGYKVGLLDADIYGPSIPKMFGIEDARPFLENING
                     KDMIIPVEKYGVKILSIGFFIDPESPLIWRGMMASNALNQLITDAYWGKLDYFIMDLP
                     PGTSDIHLTLVQTIGITGAIVVTTPQEVALTDARKAINMFRDEKINVPLLGLVENMSW
                     FTPAELPQNKYYIFGKEGGKKLAEEFNIPLLAQIPIVESIRVSSDAGEPLALNENRIL
                     STVFRELAENVVKNTNIRNATMAKTKKVEIIQKDK"
BASE COUNT         2034 a          889 c         1065 g         1753 t
ORIGIN      
        1 tttttgtccc tttccaggaa taaacatttt taacattaac ttttaaaaac aaaacaaatg
       61 aaaaaagtga aatttttgtt ggcatcttta gtgattatcg ctgcttcaag tttaaatgca
      121 caaggttttg gtgtgcaagc aggttacagc aattctacta cttttattgg tgaaggtgat
      181 accctgtcaa acctgaatgg ttttcatgtt ggtccaatag cagaactaac aattcaaggt
      241 cctttcagct tacagtatgg tttgttttat aactatcaaa ccaaacagga aacttatgaa
      301 ggtgtggtca ctgcaaaaac atccacaact tttcattctg ttgatatccc tgtacatctt
      361 gcatttacta ttccgacggg ttctcctgtt ggtgtttttg tttttggggg tcctagtttc
      421 aatgttggta tcagcaaaaa aactacatct aaagtgactt cttcattagg gacatttgaa
      481 ccaccaacta ttgattatta caaaaccgat gataacggag acagtcgtca atctcgcttt
      541 gatattcaaa tgggtgttgg cgctggcatt aagttacaga acttgcaatt gcggttgagt
      601 tatgattggg gattgctgga cacgaacaac agcgacaact atagtcttac tcgcgatgag
      661 ctgaaagctt ccattgcctt tgttttgtag gaaagcattt attaaaaaac aacaagtaat
      721 ttttaacatt cccgtcggcg gtttcttcgt cgacggtttt tttatgaaaa aaatttttgg
      781 ataaaatgaa tccagtttaa aagaaatcga aagagattat tttgcaaaaa tatatgaacc
      841 aactcagcat aaagtatttt ttaacctctc ataatcgaat gaaaattgaa aattatcttg
      901 taaatttgca ttttatagag gatattttca aatctttatt atgttgtttt ctcaaatcat
      961 tggtcaaaag aaaataaaag aaagacttct ccagagcgtt aatgaacagc gtattcctca
     1021 tgctcaaatg ctatgtggac ctgaaggtat tggtaaattt gcattggctt tggcttatgc
     1081 tcaatatatt tgctgcgaaa acaaaaccga aacagatgct tgcggcgtgt gtccttcctg
     1141 cattaaatat caaaaactca ctcatcctga tttacatctg gtattcccta tcatcaaaca
     1201 agagaaaaaa aaattagtag tttgtgatga ttacgtagca caatttcgga aaatggtcct
     1261 ggacaatcct tacttcagtg aaaatgaatg gtacgctaaa ataggtagtg agaacaagca
     1321 aggaatgatt tataccagcg aaagtgaaga aatcatcagg aaattaaatt taaaaactta
     1381 cgaagctgaa tataaagtaa tgattatctg gctgccagaa agaatgaatc ttgaaactgc
     1441 caataaatta ttgaaaattc tcgaagaacc acctgaaaaa accgtttttc ttttggtttc
     1501 aaattcgcct gataaaataa ttcctactat tttatcgcga acccagcaaa ttcagatacc
     1561 acgtttaaat aaagaagaaa ttattgaagg attgataaaa aacgaacata ttgctgaaga
     1621 agaagctttt aatgtagctc agatttcaga aggaagttat ttgaaagcat tatcctattt
     1681 gaatgaagac gatattaata agcagaattt tgaacatttt gtgaagatta tgcgtttagc
     1741 ctggaagtta gggaataaaa aagattatgc tgctttgaaa agcttgaaaa attgggcaga
     1801 agaaatggct actacgtcga atggcggaag ggaacgacag aaaaattttc tgacttatgc
     1861 tcaaagaatg attcgtgaaa attttatttt taacctgaat gaacctgcat tgaattacat
     1921 gacttccttt gaaaaagatt tttcatcggg tttttcacct ttcatcaacg aacgaaatgt
     1981 tgaggagttg atgaatgaat ttgctttagc agaaaatcat attgaacgta atgtaaatcc
     2041 taaaatggta ttttttgatt tgctaatgaa agtagttatg ctgttgaaaa aataaattgt
     2101 aatttcacat tggcattttc aaaaagatat tttttaataa attcttgttt ttagtttatt
     2161 ttagatatga aatacaaatt agatattggt ggctataata ttaacaaaaa aggatgtcac
     2221 cgtaccagtg ccctcaaatt gagtgcaatt gactggatgt gtgatcttcc tgagtcagta
     2281 aaagaaactg attttgttga agttcaattt aaaaacacac gaaaaggtta ttatctgaac
     2341 agcaataaaa ttccattgga aaaaggagat atggtggcag tagaagcttc tcccggccat
     2401 gatataggag aagttacact ggttggaaag ctggtgttac tgcaaatgaa aaaaaataaa
     2461 attgatcctg agaaaataga aattaagcgt atttatcgaa aagcgaaaga aaccgacctt
     2521 gaaaaataca aggaagccaa aaataaggaa cacagtacca tgattaaagc ccgtcagatt
     2581 gctgaaaaga tgaatctgaa aatgaaaatt ggcgacgtag aatatcaggg agatggcagt
     2641 aaagctattt tttattacat tgctgatgaa cgtgtagatt tcagagaatt aataaaagtt
     2701 tttgccgaaa cttttcacgt gcgtattgaa atgaaacaaa tcggagctcg tcaagaagct
     2761 ggacgcattg gcggaattgg tccctgcgga cgagaattgt gttgtgcgag ctggatgact
     2821 aattttaatt cagtagtcac aagctctgcc cgttgtcaag atctgtcgtt aaatccacaa
     2881 aaattggcag gtcagtgttg taagctgaaa tgttgtatga attacgaact tgactcttat
     2941 caggatgcca taaaagattt cccttcaaaa gaaattcaat tggaaacctt agacaatact
     3001 tattttcatt ttaaaaccga tgtgtttaaa ggattaatat cttattccac tgctcaaaat
     3061 atggggacta acctcattac tatttcagtt caacgagcaa aagaagtcat agctctcaat
     3121 aagcaaggca ttaaacctga caaattggaa gaccaagtat ccgaagaaat agctcaacaa
     3181 gagcaaaata cagaaaatta tgtatgccaa gataatatta ctcgctttga cgaaaaaaga
     3241 aattatcatc agcataaaaa taaaggaaaa aataaaaaaa agcaggcaaa ttcaaccaat
     3301 cagtcactaa agttgagaaa aaacgcagaa aacaatagaa caaatgaaaa taaagaagat
     3361 aagtaaactt ataattttct tttttgtagg aataatagga acagcttgcg ttcatcatga
     3421 aacttttctg gaatacaaaa ctttacctcc acaaggctgg aataaagata gtgttctgac
     3481 ttttcaagta aatatcacag acacttcttc actttataat gtctatataa acatccgtaa
     3541 tagaggggaa tatccttatc agaacttatg gctttttctt aacattcagc caccacatag
     3601 tacgttgcct atcaaagaca cgatagaatg ctatttggct gatattcatg gaaaatggct
     3661 tggctccggt gtaggcaatg tttttgaaat gcctgtctta tacaagcaaa atgagtgttt
     3721 taaacaaagt ggcacttata cttttcaaat tattcaggga atgagaaatg aagtacttcc
     3781 gggcatcagc aatgttgggc tgaaagtcga aaaaatttct tcacccaaaa aacacaatta
     3841 aagtgagcag aaataaatta tcgaaatttg cggatatgaa ttcatttcct catgtttttc
     3901 aatttcctgc ttatcctttt taccgaagtg ataccgaatt caatttaaaa ggaaaatgga
     3961 acaaggattt ttttcaaaat gattttccaa ttattctcga attagggtgc ggaaaaggag
     4021 aatacaccat tggactggca gaactttatc ctgaaaaaaa cattattggc gttgatatca
     4081 aaggagcacg aatgtggaca ggtgccaaat attcgtttga aaagcaaatg aaaaatgtgg
     4141 cttttttacg gacgaacatc gaagctgttt attatttttt tgatgagaac gaagtaagtg
     4201 aaatctggtt gccttttcct gatccacaaa tgaaaaaagt tcgaaaaagg ttgacttcta
     4261 ctcattttat gaaaatttat cagcaatttg ttaaacccgg tggacttatt catttaaaaa
     4321 cagacagcaa ttttatgttt acttacactt gcgaaatggt aaaaatcaat cattatcctg
     4381 ttagatgtat aacagataat gtttataatt ctgacaacaa tgatcctgct cttaaaattc
     4441 aaacctttta tgagtcacaa tggatagaaa gaggattaaa aattaaatac ttacaattca
     4501 atttagaaaa aagagaaaca ttttttgagc ctgatattga aattgaacgt gatacatata
     4561 gaagctttgg tagaaacaaa aattagttta tgtattttac attaatcagg tcaacattat
     4621 tcaaattcaa ttatggaaat ttacccaaag ttaattcttg atgcattaag tcatgtaaaa
     4681 tatcctggaa aagggaaaga cattgtttct ctcgagatgg tacaggataa tattcgtatt
     4741 gaagggaaca aagtcagttt ttcgctgatt tttgataaac cgaacgatcc ctttataaaa
     4801 tcagttgtga aagctgctga acaagctatt ttaacttatg tttcgccaga agtggatatc
     4861 aaaggcaaca ttacaccgat taccaaagaa gtacccaaac cagctcccgt tttgccagaa
     4921 gtagaaaata ttattgccat tttttctggg aagggtggtg tagggaaatc cactattgca
     4981 gcgaatttag cggctgctct tgcttcttta ggttacaaag tgggtttatt ggatgccgat
     5041 atttacggtc cttctattcc caaaatgttt ggaattgaag atgctcgtcc tttcttagaa
     5101 aatattaatg ggaaagatat gattattcca gttgagaaat atggagtaaa aattctttct
     5161 ataggctttt ttattgatcc tgaaagtccg ttgatttgga gaggtatgat ggcaagtaat
     5221 gctcttaatc agcttattac agatgcctac tggggaaaat tggattattt catcatggat
     5281 ttaccgcctg gaaccagtga tattcactta actttggtac aaacaatagg aattactgga
     5341 gcgattgttg ttaccacccc tcaagaagta gcattgaccg atgcacggaa agctataaat
     5401 atgtttcggg acgaaaaaat caatgtgcca cttctgggac tggtcgaaaa tatgtcgtgg
     5461 tttacgcctg ctgaattgcc acaaaataag tattatattt ttggaaaaga aggaggaaaa
     5521 aagttagcag aagaattcaa cattcctctt ttggcacaaa tacctatagt agaaagtatc
     5581 agagtatcaa gtgatgctgg tgagccactt gccctgaatg aaaatcgtat tctttctact
     5641 gtttttcgtg aactggcaga aaatgtagta aaaaacacga acattagaaa tgcaacaatg
     5701 gcaaaaacca aaaaagtaga gattatccag aaagataaac a
//
LOCUS       DAOTKS010000054         5640 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_12970_length_5640_cov_3.875918, whole genome shotgun sequence.
ACCESSION   DAOTKS010000054 DAOTKS010000000
VERSION     DAOTKS010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12970_length_5640_cov_3.875918"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1214
                     /locus_tag="P5236_01865"
     CDS             <1..1214
                     /locus_tag="P5236_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HRU63139.1"
                     /translation="LDKLGRRHKILAIPPDYTRLPSRAGELTEMVWEYYGNTLTDILP
                     ALGTHTPMTDEQISHMFGKTPRNLFRVHDWRHDVITLGEVPAEYVKEVSEGKVDFSWP
                     AQVNKLLVEGNFDLILSIGQVVPHEVVGMANYNKNIFVGTGGIEGINKSHFVGAVYGM
                     ERMMGRADTPVRKLFNYASKNFANQLPIVYILTVVGINKEGKQQTYGLFVGDDVDVFD
                     EAAKLSLEVNFEMVEEPLKKILVWLDPTEFKSTWLGNKSIYRTRMAIADGGELIVLAP
                     ALKEFGEDKEIDRLIRKYGYHGTSATLKAIAENEELRNNLSAAAHLIHGSSEGRFTIT
                     YCPGKNHENLTKEEIESVGFQWADIDEVMNRYHFRELKEGYNILPDGEEIFFISNPAL
                     GLWAHKDRFEF"
     gene            1783..2307
                     /locus_tag="P5236_01870"
     CDS             1783..2307
                     /locus_tag="P5236_01870"
                     /inference="COORDINATES: protein motif:HMM:NF040109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6452 family protein"
                     /protein_id="HRU63140.1"
                     /translation="MNKMSYLQKYSLRICWMLILWSIGNSCSTTDEFCREEKNVKLKM
                     SFYTRNFNSNTQKYVNSSLSVDSLTIVGIDSLGQLIDSVLYNNSKNISSVKLPLNKFS
                     KESKFLLILNDELDTITIHYTNHLQYLSFECGSIITHTIDTVYTTSHFIDSLTIVEPD
                     ITTLDVENLQIFHY"
     gene            2276..2959
                     /locus_tag="P5236_01875"
     CDS             2276..2959
                     /locus_tag="P5236_01875"
                     /inference="COORDINATES: protein motif:HMM:NF039966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6048 family protein"
                     /protein_id="HRU63141.1"
                     /translation="MLKICKYFIISFLIFFFSHINVFSQNKKENLPKIKWWNGIMLNF
                     DVVSPIQSSLSSSQNFSAVGNVQINLKDIFLPVYEIGIDGINKTSSQEIYYKANGIFQ
                     RAGIDFNLLRQKKEAEPSNNKLLGGIRVGWSNFKYDVTNITITDDYWGGESILNYERQ
                     SASKVWYEIVFGIQVEVFKNVYLGWNIRSKNLFKQNTSTEVSPWYIPGFGKNNTSNVG
                     FNYTVGYHF"
     gene            3008..5032
                     /locus_tag="P5236_01880"
     CDS             3008..5032
                     /locus_tag="P5236_01880"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRU63142.1"
                     /translation="MNSIETLNRAADNIRILAAAMVEKAKSGHPGGAMGGADFMNVLY
                     SEFLEYDPENPFWEGRDRFFLDPGHMSTMLYAVLAFSGKFTLDELKQFRQWGSPTPGH
                     PELDIKRGIENTSGPLGQGHTYAVGAAIAAKFLKARFGEVMDHTIYTFISDGGIEEEI
                     SQGAGRIAGHLGLDNLIMFYDSNNIQLSTETKVVTSENTAQKYEAWGWKVITIDGNDA
                     AQIRKALTEAKAEKERPTLIIGKTIMGKGIKKADGSSFENQISTHGMPISEAGGSFEE
                     TIKHLGGDPANPFVIFEETKTLYAKRAEELKEIVSKKQAIKNSWAKENPQLAEKLKFF
                     FSSEIPPVKWDAIVQTPNKSTRAASSVVLAELAKQVENMIVASADLSNSDKTDGFLKQ
                     TKAFAKNDFSGAFLQVGVSELTMAALCVGMSLHGGVIPACGTFFVFSDYMKPVVRMAA
                     LMEQPVKFIWTHDAFRVGEDGPTHQPVEQEAQIRLMEKLKNHSGNNSMLVLRPADAEE
                     TTVVWKLALENTKCPTALIFSRQDIVSLPAIENRRKEAEQASRGAYVVQCDGNPEIIL
                     VASGSEVSTLAEAAVLLRKDGIKVRLVSVPSEGLFRLQPITYQERVLPPNSKIFGLTA
                     GLPVTLQGLVGNNGKIFGMESFGFSAPYKVLDEKLGFTPQNIYEQIRKIL"
     gene            5096..>5640
                     /locus_tag="P5236_01885"
     CDS             5096..>5640
                     /locus_tag="P5236_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HRU63143.1"
                     /translation="MDIVSIILIALGLAMDCLAVSISKGIAVRKFYFSSTLLMAFLFG
                     FFQAVMPLIGYFAGLNFLTVIKNSDHWLAFVLLLLIGGKMLWEGIRNHSSEKDKGETH
                     PFHWMKLLTLSLATSIDALATGIIFVPYPLMVWKAITIIGTISFLMSILGVYIGVRFG
                     KRFHVNVEILGGIILIGIGFK"
BASE COUNT         1856 a          898 c         1094 g         1792 t
ORIGIN      
        1 cattggacaa attaggcaga agacataaaa ttcttgccat tcctcccgat tacacccgct
       61 tgccaagtcg ggcaggagaa ctcaccgaaa tggtttggga atattacggc aacacactta
      121 ccgatatact cccggcatta ggcacacaca ctcctatgac agatgaacaa atttcgcaca
      181 tgtttggaaa aacaccacga aatttgtttc gtgttcacga ttggcgtcac gatgtaatca
      241 cattgggaga ggtcccagca gaatatgtaa aagaagtttc cgaagggaag gtagattttt
      301 cttggccggc acaggtaaat aaattgctgg tggaaggtaa ttttgacctg attctttcca
      361 taggccaggt ggttccacac gaagtggtcg gcatggccaa ttacaacaag aacatttttg
      421 taggcacagg tggtatcgaa ggaattaaca aaagccattt tgtcggtgcc gtttatggga
      481 tggaacgcat gatggggcgc gcagataccc cggtaaggaa actgtttaac tatgcctcaa
      541 aaaattttgc caatcagctg cctattgttt atatactgac agttgttggc ataaacaagg
      601 aaggcaagca gcagacttac ggactttttg taggtgacga tgtggatgta ttcgatgaag
      661 cagcaaagtt gtctcttgaa gtgaatttcg aaatggtcga agaacccctg aaaaaaatcc
      721 ttgtttggct cgaccctacc gaattcaaaa gcacgtggct ggggaataaa tctatctatc
      781 gcacccgcat ggctattgct gatggtgggg aacttattgt tttggcaccc gccttaaaag
      841 aatttggaga ggacaaagag attgaccgcc ttatcagaaa atacggctat catggaactt
      901 ccgctacctt gaaagccata gctgaaaacg aagaattacg gaataattta agtgctgcag
      961 ctcatttgat tcatggctcg tcagaagggc ggtttactat cacttactgc cccggcaaaa
     1021 atcacgaaaa cctgacaaaa gaagaaatcg aaagcgtggg tttccaatgg gcagatatcg
     1081 atgaggtgat gaaccgttat cactttcgtg aactcaaaga gggatacaac atcttgcccg
     1141 atggagaaga aattttcttt atctccaacc cggcattggg actatgggca cataaagatc
     1201 gatttgaatt ttgaatttaa actctcaagt tttggaataa ttaattctga catttattac
     1261 ttgaaagtac ttaataaatc caaatttgtc attaggtaaa atttacacta acgattatta
     1321 gaaaataggt ataccaactt acaaccagaa tcaaaattaa ataaagaaaa caacgacctt
     1381 tgacatcaaa taatatacga ttgtattttg ttttaatagt gagtttgcgc tgattcttca
     1441 cttgctaagt tttacgtaga tttgtaatct gcagatacgg aaggtaaaaa ataatggcac
     1501 aaattgcaaa tttatgtcag aaaaaatttt taatgttaat caacaaaggt attaagtatc
     1561 cgcctccata aaaaattttc cttttttcgc actacatacc ttgaacttcc tccgtttttc
     1621 agcttagtag acaagattaa ttacttttct tttaccattt tcagcaaaat catttttctt
     1681 tttgaaaaca agttggtgta attgactatc aaagcaaaat acaaaataaa gaaaggtttt
     1741 cgttaatttg ccagtgaata tgtatatttg tatcgaaaat taatgaataa aatgagttat
     1801 ctccaaaaat atagtttacg tatttgctgg atgttaattt tatggagcat cggaaattct
     1861 tgttccacca ctgatgaatt ttgccgggaa gaaaagaatg ttaaattaaa aatgagtttt
     1921 tatactcgta attttaatag caatacacaa aaatatgtca attcttcctt atctgtggat
     1981 agcttgacaa tcgttggcat cgattcttta ggacaattaa ttgactcggt tttatataac
     2041 aattcaaaaa atatttcatc agttaaattg cccttaaata aattttcaaa ggaatcaaag
     2101 tttctattaa ttttgaatga tgagttagat accataacaa tacattatac caatcattta
     2161 cagtaccttt catttgaatg tggaagtatt atcactcaca ctattgacac tgtttatact
     2221 acttcacact ttattgattc cctgacaatt gttgaaccag acataacaac tcttgatgtt
     2281 gaaaatttgc aaatatttca ttattagttt tttgattttc ttttttagtc acataaacgt
     2341 tttttctcaa aataaaaaag aaaatttacc taaaattaag tggtggaatg gaattatgtt
     2401 gaattttgat gtcgtttctc ctattcaatc ctctttatct agttcacaaa atttctcagc
     2461 ggtaggaaat gtacaaataa atttaaagga tattttttta cccgtttatg aaataggcat
     2521 tgatggaatt aataaaactt cttcacaaga gatttattac aaagctaatg gaatttttca
     2581 acgtgcaggc atagatttta atttattaag acaaaaaaaa gaagctgaac caagtaataa
     2641 caaattgtta ggtggaattc gagttggatg gtcaaatttt aagtatgatg tgaccaatat
     2701 tactattaca gacgattatt ggggtggaga aagtatatta aattatgagc gtcaatcagc
     2761 ttctaaagtc tggtatgaaa ttgtttttgg aattcaagtg gaagtattta aaaacgtata
     2821 tttagggtgg aacattcgaa gcaaaaactt gttcaaacaa aatacttcaa cagaagtttc
     2881 tccatggtat attccaggtt ttggtaaaaa caatacttct aatgttggat ttaactatac
     2941 tgttgggtat catttctaat aacttaaaaa tgaatatatt gaataaaaac aacaaaacta
     3001 tatttttatg aattcaattg aaactttaaa tcgggcggct gacaacattc gtattttggc
     3061 agcagcaatg gtagaaaaag caaaatcagg acatcctgga ggcgccatgg gaggtgccga
     3121 ttttatgaat gtgctttatt ctgaatttct tgaatatgat ccagaaaatc ctttttggga
     3181 aggacgcgat cgtttttttc ttgatccagg gcacatgtca acgatgttat atgcagtact
     3241 tgctttttct ggaaaattta cattagacga attaaaacaa ttcagacaat ggggaagtcc
     3301 taccccggga caccctgaac tggatataaa aagaggaatt gaaaatactt ctggtccgct
     3361 tgggcaagga catacatatg ctgtaggagc agccatagca gcaaaatttt taaaagctcg
     3421 ttttggtgaa gtaatggatc ataccattta tacgtttata tctgatggag gcattgagga
     3481 agaaatttca cagggtgctg gacgtattgc tggtcattta ggattggata atttgattat
     3541 gttctatgat tcaaataata ttcaactttc taccgaaacc aaagtggtta cgagtgaaaa
     3601 tactgctcaa aaatatgaag cttggggctg gaaggtgatc actatcgatg gcaatgatgc
     3661 agcacaaatt cgaaaagctt tgacagaagc caaagccgag aaggaacgac ctacgttaat
     3721 tatcggaaaa actattatgg gaaaaggaat aaaaaaggca gatggttcaa gttttgaaaa
     3781 tcaaatatct actcacggta tgcctatcag tgaagcaggg ggttctttcg aagaaacaat
     3841 aaaacatctc ggaggagatc ctgctaatcc ttttgttatt tttgaagaaa caaaaacttt
     3901 atatgctaaa cgtgcagaag aattaaaaga aattgtttca aaaaaacaag ccattaaaaa
     3961 ttcttgggca aaagaaaatc ctcaattggc tgaaaagtta aaatttttct tctccagtga
     4021 aattccacct gtaaagtggg atgctattgt tcaaacccct aacaaatcta ctcgtgctgc
     4081 ttcttcagtg gtattagctg aattggcaaa gcaggttgaa aatatgattg tagcaagtgc
     4141 tgatttatcc aattctgata aaactgatgg atttttaaag caaaccaaag cttttgcaaa
     4201 aaatgatttt tcaggtgctt ttttacaagt aggtgtttct gaactcacta tggctgccct
     4261 ttgtgttggg atgagtttac atggaggcgt cattccagct tgtggaactt tctttgtttt
     4321 ttctgattat atgaaaccag tagtacgtat ggctgctttg atggaacaac ctgttaaatt
     4381 tatctggaca cacgatgcat ttcgtgttgg agaagatggt cctacgcatc aacctgtgga
     4441 acaggaagca caaattcgct tgatggaaaa attgaaaaat cacagtggaa ataattcgat
     4501 gttggtgctt cgccctgccg atgcagagga aacgacagta gtatggaaat tggctttgga
     4561 aaataccaaa tgtccaactg ctttaatctt ttcacgccaa gatattgtga gccttccagc
     4621 tattgaaaat cgcagaaaag aagctgaaca agcaagcagg ggagcttatg tagttcaatg
     4681 tgatgggaat ccagagatta ttcttgtagc ttcaggttct gaagtttcta ccttagctga
     4741 agcagcagta ttacttcgga aggatggtat taaagtacgt ttagtgtccg taccttctga
     4801 aggtttgttc cgcttacaac caattaccta tcaggaaaga gttttaccac ccaatagtaa
     4861 aatttttggt cttacagcag gactcccagt aacacttcaa ggtttggtag gaaataatgg
     4921 aaaaattttt ggaatggaaa gttttggttt ttcagctcca tataaagtat tggacgaaaa
     4981 acttggtttt acccctcaaa acatttacga gcaaatcaga aaaatattat aattaatttt
     5041 tcaacaaaag taagaagaaa ataatacaca ctcacttact tttttaattt atattatgga
     5101 tattgtctct attattctaa ttgctcttgg attagcaatg gattgtttgg cagtttctat
     5161 cagtaaagga attgcagtaa gaaaatttta tttttcatca actttattaa tggctttttt
     5221 gtttggcttc tttcaggcgg tgatgccttt gattggttat tttgctggat tgaattttct
     5281 tacagttatt aaaaatagtg atcactggtt ggcttttgtc ttgctgttat taataggagg
     5341 aaaaatgttg tgggaaggta taagaaatca ctcttcagaa aaagataagg gagaaactca
     5401 tccttttcac tggatgaagc ttttaacctt atcattggca accagcattg atgctttagc
     5461 taccggaatt atttttgttc cttatccatt gatggtttgg aaagcaatta caattattgg
     5521 aactattagt ttcttaatga gtatattagg tgtgtatatt ggagtacgtt ttgggaaacg
     5581 ttttcatgta aatgtagaaa ttcttggtgg aattattttg attggaatcg gtttcaaaat
//
LOCUS       DAOTKS010000055         5634 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_12984_length_5634_cov_4.143753, whole genome shotgun sequence.
ACCESSION   DAOTKS010000055 DAOTKS010000000
VERSION     DAOTKS010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5634
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12984_length_5634_cov_4.143753"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="P5236_01890"
     CDS             complement(<1..1134)
                     /locus_tag="P5236_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HRU63144.1"
                     /translation="MKKTLQTPDKEITILSTEEFPMIAKTFQGLEEVLAEELTSIGAS
                     NVQIQRRAVSFTGDKALMYKANLHCRTALRILKPIFTFNANDADEVYEQIKKFDWGQL
                     MTVESTFAIDATVFSEEFTHSKYVVYRVKDAIADWFMERYHRRPSVSVTSPQLMFNIH
                     IAGRHCTLSLDSSGESLHKRGYRVAQTEAPINEVLAAGMLLLAGWHGQSDFLDPMCGS
                     GTLLIEAALIALNMPPGIFRPSFAFEKWSDFDKELFDELYNDDSQEKTFEHKIYGSDV
                     SARAMKIAEQNVKSSGLSKYIQLKAVPIEKLEPPSSHCFIVTNPPYGERLPSKDIFDL
                     YASLGSVLKHKFAGSTAWIISSNEEALKKIGLKPSKRIQLLNGP"
     gene            complement(1147..2037)
                     /locus_tag="P5236_01895"
     CDS             complement(1147..2037)
                     /locus_tag="P5236_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HRU63145.1"
                     /translation="MKNEILETIQKLSQIEKIKDVCHKQGLNFPMPSINTLQEIVQLC
                     RSILFPGYFDEAPLNSETLKFHIGVNVDHLLMLLSKEIKAGICFNGNTKITTEKCEEL
                     DKISLQKAVEFILKLPEIRQKLYTDVIAIYNGDPAAKSFGEVIFCYPGLKAITNYRLA
                     HALLKLDVPLIPRIITEMAHSETGIDIHPGAEIGEYFMIDHGTGVVIGETSVIGNNVK
                     MYQGVTLGAKSFPLDENGNPIKGIVRHPKIGNNVIIYSNSTILGNITIGDGAVIGGNL
                     WVDKDVPAGARLSQDKNLNF"
     assembly_gap    2108..2207
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2535..3182
                     /locus_tag="P5236_01900"
     CDS             2535..3182
                     /locus_tag="P5236_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRU63146.1"
                     /translation="MCKFTTFLLIFIILSLYLSAQNPDIWILEQVNDEHPKMLRKYSL
                     MVTNTATTITLSTPFVIGMIGIIDKNSQLEKDALYVGATLGVEGILTLGLKQAVHRER
                     PYVTYPNVVFPYQILPSYSFPSGHTSMAFANATALSLKYPKWYVIIPSYFWACSVGYS
                     RLNLGEHYPSDVLAGAIVGAGSAFLSLKLTNWLFNSCENKKQCKTLNIFNTYLSF"
     gene            3863..4873
                     /locus_tag="P5236_01905"
     CDS             3863..4873
                     /locus_tag="P5236_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007486243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRU63147.1"
                     /translation="MKNTQITIKDIAKALNISPSTVSRALKNHPDISEKTKKAVQEYA
                     QKFNYKPNVLASNLRMSKNNTIGVIVPEIIHYFFSSVLSGIEQVSNREGYNVIICQSS
                     ENYEKEVRNVDALISSRVAGVLACLSKTTSIYDHFQEIINHSIPLVFYDRIYIGINTD
                     RVVVDDYTGAFSAVEYLIQTGCKRIAFYSSPLHLEISKNRRNGYLDALYKYGLPVDES
                     LIRICDTREEAIIITPEILDRPDRPDGFFAINDHCASGILYATKLAKLSVPEDVSICG
                     FSDGELAKACDPMLTTVEQHGYEMGINAAELLISKIKGETQGKYTNKIIKTNLIIRGT
                     TR"
     gene            5198..5455
                     /locus_tag="P5236_01910"
     CDS             5198..5455
                     /locus_tag="P5236_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63148.1"
                     /translation="MDSFRKTETFFSCPSINVAMEPFRALVADMLPEEQSTLGFSIQS
                     FLIGIGAVIGSWLPYVLAEWFGIPKTTINGNIPSNVIYSFH"
BASE COUNT         1875 a          893 c          861 g         1905 t
ORIGIN      
        1 aggtccattc aacaactgaa tacgtttcga tggttttaaa ccaatttttt tcagtgcttc
       61 ttcgttagaa ctaattatcc atgcagtgct tccagcaaat ttatgtttta acactgagcc
      121 taatgaagca tacaaatcaa aaatatcttt tgacggaagt cgttcaccat agggaggatt
      181 ggttacaata aagcaatgtg aagatggcgg ttcaagcttt tcaataggga cagcttttaa
      241 ttgaatatat tttgaaagtc cgctgctttt tacattctgt tccgcaattt tcatagctct
      301 tgcggaaacg tctgaaccat aaattttatg ttcaaaagtt ttttcttgcg aatcatcatt
      361 gtacaactca tcaaaaagtt ccttatcaaa gtctgaccat ttttcaaaag caaaagaagg
      421 tctgaaaatg cctggtggca tatttaatgc tatcaaggca gcttctataa gcaatgtacc
      481 agatccgcac atgggatcga gaaaatcgga ttgcccatgc cagccagcca ataacaacat
      541 tcctgctgcc agcacttcgt taataggagc ttcagtttgt gcaacacgat aaccacgttt
      601 gtgaagcgat tctccagaac tgtccaatga aagggtgcaa tgacggccag caatatgaat
      661 attaaacatt aattgtggcg aggtaactga aacactcgga cgacgatgat aacgttccat
      721 aaaccaatcg gcaatagcat cttttactct gtaaactaca tattttgaat gtgtgaattc
      781 ttctgaaaat actgttgcat caatagcaaa tgtgctttca actgtcatta attgacccca
      841 gtcaaatttt tttatctgtt catatacttc gtcagcatca ttagcattaa aggtaaaaat
      901 tggcttcaaa atacgtaagg ctgttcgaca atgtaaattt gctttgtaca tcaaagcttt
      961 atctcctgta aaactgactg ctcggcgttg tatttgaaca ttactggcac caattgaagt
     1021 aagctcttct gccaaaactt cttccaatcc ttgaaaagtt ttggcaatca ttggaaattc
     1081 ttctgtagaa agtatagtaa tttctttatc tggagtttgt aaagtttttt tcattaaaaa
     1141 taaaaatcaa aaattcaagt ttttatcctg tgataatctc gcaccagccg ggacatcctt
     1201 atcaacccag agatttccgc caatcactgc tccatctcca atagtaatat ttcccagaat
     1261 agtagaattg gaataaataa tcacattatt tccaattttt ggatgtcgaa caatgccttt
     1321 gataggattt ccgttttcgt ccaatggaaa gctcttggct ccaagcgtaa ctccttgata
     1381 catttttaca ttattaccaa tgacagaagt ttcaccaatg acaacacccg ttccatgatc
     1441 aatcataaaa tattctccaa tttctgctcc cggatgaata tcaatacctg tttctgaatg
     1501 agccatttct gtgatgattc gtggaattaa gggaacatca agtttcagta aagcgtgagc
     1561 cagtcgataa ttggtaattg cttttagtcc aggataacaa aaaataactt cgccaaaact
     1621 ttttgcagca ggatcgccat tataaatagc aatcacatcg gtataaagtt tttgccttat
     1681 ttcaggcagt ttcaatataa attcaacagc cttttgcaga gaaattttat ccaactcctc
     1741 acatttttcg gtagttattt tggtatttcc gttaaaacat attcctgctt tgatttcttt
     1801 tgaaagcagc atcaacaaat gatcaacatt cactccaata tggaatttta atgtttctga
     1861 atttaaagga gcctcatcaa aatatcctgg aaagagaata gaacgacaca actgaacaat
     1921 ttcttgcaat gtattgatgg aaggcatagg aaaatttaat ccttgtttgt ggcagacatc
     1981 cttgattttt tcaatctggg ataacttttg aatagtttct aaaatttcat ttttcatatt
     2041 tttatatttt tatttattac gaaatgataa taaggcattt atagcacatt gaatccgttg
     2101 atcaccannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttc tgccagcttt
     2221 tttgctaatt ctgtttttcc tgttgattca ggaccaataa tggcaatttt tttcataaag
     2281 taatcaattt gtctatctgt aatttttttc ccttttaaaa accatacaac taaaaattgt
     2341 gttacactag agtaaatttt aaattgttag aatgaatatt taaaggtagt tttttacttt
     2401 aaattaacgt tttattaaaa gatgaaaaat tccatagcaa aaaatttaaa caggagtaaa
     2461 aagtgaaata tcttagttaa aattctgatt tctaaaaaca tcaattctat aatctactta
     2521 gattcatcaa aaatatgtgc aaatttacaa catttctatt gattttcatt attttatctt
     2581 tatacctttc tgctcaaaat cctgatatct ggattttaga acaagtaaac gatgaacatc
     2641 ccaaaatgtt gagaaaatat tctctcatgg taacgaatac cgctacaact attacacttt
     2701 ctactccttt tgtgatagga atgataggaa taattgataa aaactcacaa ttggaaaaag
     2761 atgcattata tgttggagct acattaggtg ttgaaggaat attaactttg gggttaaaac
     2821 aagctgttca cagggaacgt ccttatgtaa cttaccccaa cgtagttttt ccttatcaaa
     2881 tcttgccttc ttattctttt ccatcggggc atacttctat ggcttttgcc aatgctactg
     2941 ctttgagttt aaaatatcct aaatggtatg tgataattcc aagttatttt tgggcttgct
     3001 ctgtcggcta ttcccgacta aatctgggcg aacattatcc ttctgatgta ttagctggcg
     3061 ctattgtagg agctggaagt gctttcttat ccttaaaatt gaccaattgg ttatttaatt
     3121 catgcgaaaa caaaaaacag tgtaaaacac ttaatatttt taatacttat ctttcttttt
     3181 gatactcagt tgtattcgag aatataaagc tttgaaataa tatgatgcat gacattattc
     3241 agaaaaaaga gaaggaattt tttcaaggta aaacattgct gtgaacttat ttccttttgc
     3301 ctcaaaaggg tttcatttta attgttttag caaattttgc tcctctttaa tcaaaagaat
     3361 tcctcgaggt ttgccttggg gttaattttg ctaaaaaata gataatttat ttgttaaatt
     3421 attcacttac tgagaagtaa agtttgtcac attatttagt agcaaatata taggttaaaa
     3481 atatatttac aactcccaaa aagttaaatc aataaaaaaa ttatgcaaac gattgcaatt
     3541 attttttttc aagaaataat ttttttctaa ataaaactat tttatttttg taaaaagtat
     3601 catttggtag aaaattctat ataagaactt attttttcaa tcattttgtt aaaagtattc
     3661 aacttattat tttgattaag taactaacat tttttggaaa aacaaaatag taatttgttc
     3721 cgaaaaatca attttaataa tttcttgatt aatatgatat tgaatattaa tttaatatta
     3781 aatttcaaca gatgagaaat aaaacaaact tttagcatat acttctttat tttacaataa
     3841 ggtaagattt taaaaaatta tcatgaaaaa tacacaaatt accattaagg atattgcaaa
     3901 agcccttaat atctctccat ccactgtatc tagagcttta aaaaaccatc ctgatattag
     3961 tgaaaaaaca aaaaaagcgg ttcaagagta tgctcaaaaa tttaattaca aacctaatgt
     4021 acttgcttca aatttacgaa tgagtaaaaa taatacgata ggagttattg ttccagagat
     4081 tattcattat tttttctctt cggttttgtc aggaattgaa caagtatcaa acagagaagg
     4141 atacaatgta attatttgcc agtcaagcga aaattatgaa aaagaagtac gaaatgttga
     4201 tgctctaatt tcttccagag ttgctggagt attggcatgc ttatcaaaaa ccacttctat
     4261 ttatgaccat tttcaagaaa ttattaatca tagcattcca cttgtttttt atgaccgtat
     4321 ttatattggt atcaataccg atcgtgtagt agttgatgat tatacgggtg ctttttctgc
     4381 tgtagaatac ttaattcaaa ctggttgtaa aaggatagct ttttattctt cacccttgca
     4441 cctggagatt tccaaaaata gaagaaatgg ctatttggat gctttatata aatatgggct
     4501 tccagtggac gaatctttga taagaatttg tgatacccgc gaagaagcta ttataattac
     4561 acctgaaatt ttagatcgtc ccgatcgtcc tgatggcttt tttgccatta atgaccattg
     4621 tgcctcgggt attttatatg ccacaaaact tgccaaatta agtgtccctg aagatgtttc
     4681 catttgtggc ttttctgatg gcgaattagc aaaagcttgt gatcctatgt tgactactgt
     4741 tgaacaacat ggttatgaaa tgggtataaa tgcagcagaa ttattaatat caaaaataaa
     4801 gggggaaact caaggaaaat acactaataa gattataaaa acaaacctta ttattagagg
     4861 aactaccaga taacttaaag catatatttt ttcaaaaaat agaaataagc ataataaagt
     4921 tagaatcaat tttttgttta aattttgaaa ggatatggga gaacagcaaa atattaatat
     4981 aaaaaaagaa aaaaccaacc ttttcgtttt ttcaaatcct ttcaatgagc atgggttttt
     5041 ggggtatcca atttggatat gctttacaaa atgctaatgc aagtagaatt ttacaaactt
     5101 ttggagcaaa cattgaacaa ctttcatggt tttggttaac agcccctctt accggaatga
     5161 ttattcaacc aattattggg cattattctg atcaaacatg gactcgttta ggaagacgga
     5221 aacctttttt tcttgtcctt ctattaatgt ggctatggaa ccctttagag cactggttgc
     5281 cgatatgtta ccagaagaac aaagtacact tggcttttca atacaaagtt tcttgatcgg
     5341 aattggtgca gtaattggtt catggctacc ttacgtatta gctgaatggt ttggcattcc
     5401 aaaaacgaca ataaatggta atatcccttc caatgttatt tattcatttc actaaagttt
     5461 agcagttcag aaagggttga aaataaaaag gaaaaagagt taagaaagta aaaaagtagc
     5521 atattttatc taatatctca ttaagaatga gtatattagc tgtgtaatta caaaaacaga
     5581 aaaatatgct accagacaaa gttagaaaaa agatttctga aattgaaaca gatt
//
LOCUS       DAOTKS010000056         5630 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_12995_length_5630_cov_4.651659, whole genome shotgun sequence.
ACCESSION   DAOTKS010000056 DAOTKS010000000
VERSION     DAOTKS010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5630
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12995_length_5630_cov_4.651659"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="P5236_01915"
     CDS             complement(<1..766)
                     /locus_tag="P5236_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HRU63149.1"
                     /translation="MILSTFPIEKSLNRAYLKQDLTKKQMEVFRNELKKLLDTISQLN
                     NQGQDEENFKTPIIHFLTETYYKGKNYVNSSKKYDLVIGNGEKVADSVAVIIETKRPS
                     NTAEMITSSNPNAKALHELVHYYMQERKNGNDELKYLVVTNCYEWYLFDAVDFENIFY
                     KNQEFRNNYENWDTKQKVDNTTFSLYENIRNFIDKNVAELKATYFDLRDYETALNSDD
                     SENLSQLINLYKIFSPEHLLKKPFANDSNTLNREFYN"
     gene            complement(966..1739)
                     /locus_tag="P5236_01920"
     CDS             complement(966..1739)
                     /locus_tag="P5236_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HRU63150.1"
                     /translation="MKILILGAGKMGTFFTDLLCLHHEVALFDIDPKKLRFVFNTYRL
                     TDYEQIREFQPELVLNCVTLKFTIEAFQNVMPFLPENCMLSDIASVKTGLKEFYESTG
                     KRFVSTHPMFGPTFATLDNLSSQNAIIISESDFLGKTFFRDLYHSLHLKIFEYSFEEH
                     DKTTAYSLAIPFASTLVFAAVMKHQEAPGTTFKRHMEIAEGLLSEDDYLLTEILFNPY
                     TPEQIKKIQAELATLLDIIEKKDTERMQAFLEKVRKNIQ"
     gene            complement(1838..3706)
                     /locus_tag="P5236_01925"
     CDS             complement(1838..3706)
                     /locus_tag="P5236_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRU63151.1"
                     /translation="MKNKQTRNVSPWAWVPTLYFAEGIPYVVAMTVTVIMYKRLGISN
                     ADIALYTSWLYLPWVIKPFWSPFVDIIKTKRWWILSMQLLIGAGLAGVAFLIPVPFFF
                     QATLAVLWLIAFSSATHDIAADGFYMLALDSSQQSFFVGIRSTFYRFAMITGQGLLII
                     LAGALESHTGTKPVLLTVASTNSSQIEMMEIPENVLPADTAELSFVAYPSQLVLSTQT
                     ISRDSLNKILQYATEQNITNRFVEKEQKTVTGKSEKNSWWKRNVSDPLANYIRDHFGE
                     KREIKTDLTERVGNAGLVQIRLTKKPEKGKSLIFNITHAKGDKSISLVTGERLVFDET
                     NWDKPAYVVFQLDPKLKDEVKSEFKGVSGNVKLAWSITFFIIAGFFILIYFYHRFILP
                     YPDSDHPTLTNTAKDILREFGRTFATFFQKKGIGLAIAFMLLYRLGEAMLVKMASPFL
                     LDPREIGGLGLTTGQVGLVYGTVGVVALTIGGIIGGIVASQKGLKFWIWPMALCITLP
                     HLVYLYLSSVQPENMLLINLAVAIEQFGYGFGFTAYMLYLIYFSEGEHKTPHYAICTG
                     FMALGMMLPGMIAGWLQELLGYHHFFIFVVLCAIPTLVVIPFMKIDKEFGKKKTTT"
     gene            4409..4534
                     /locus_tag="P5236_01930"
     CDS             4409..4534
                     /locus_tag="P5236_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63152.1"
                     /translation="MSGEEASLKDFSQQTPIFPPYLTKNLPNRNDFPLNQALLPY"
     gene            complement(4594..5316)
                     /gene="recO"
                     /locus_tag="P5236_01935"
     CDS             complement(4594..5316)
                     /gene="recO"
                     /locus_tag="P5236_01935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRU63153.1"
                     /translation="MLTKTEGIVLHSTRYSDFASIVTIYTRQFGRNAYMVYGKGKKKS
                     IYKPAFFQPFSIVELDVCHSPKKEIQQIKDIKLSFLPQTLFFDPVKNSIALFLSEILF
                     KVLLQAETDEQLYSFLENSIKILDTMEEGTSNFHLIFLLQLTRFLGFKPNSDNPSKTY
                     FDLLNGTFLSHPPLHSHFLGPELTVDFILLLKSNYSTMANVLLNHQKRKELLENVLQY
                     YQLHISGLSNIQSLEVLEEVFT"
BASE COUNT         1935 a          971 c          891 g         1833 t
ORIGIN      
        1 cattataaaa ttctctgttc agtgtgttac tatcgttggc aaatggtttt ttaagcagat
       61 gttcaggaga aaatatttta tacaaattga tgagttgact taaattttca ctgtcatcac
      121 tatttaaagc agtttcataa tcacgtaaat caaaataagt agctttcagt tcagcaacat
      181 ttttatcaat gaaatttcga atgttttcat agagcgaaaa agtagtattg tccacttttt
      241 gtttggtatc ccaattttcg taattgttcc tgaattcctg atttttgtag aaaatatttt
      301 cgaaatctac tgcatcaaac agataccatt cataacaatt ggtaacgacg agatatttga
      361 gttcatcatt tccgtttttc ctttcttgca tgtaataatg caccaattcg tgcaaggctt
      421 tagcattggg attagaagaa gttatcattt cggcggtgtt ggagggtctt ttggtttcaa
      481 taataaccgc cacgctgtcg gcaactttct cgccattacc gattactaaa tcatattttt
      541 ttgaactatt cacatagttc ttgcctttgt aataagtttc tgtcagaaaa tgaataatgg
      601 gtgttttaaa attttcttcg tcttgccctt gattatttaa ttgagaaatg gtatccagca
      661 attttttaag ttcattcctg aaaacttcca tttgcttttt ggtaaggtct tgtttcaaat
      721 aagcccgatt taagcttttt tctataggga aggttgacaa tatcatggtt ctgttttttt
      781 acaaatgtag aattaaaagg ttatttttca aaatgttttt tgaggtcagg gtacaaaaaa
      841 tgtgttcagc gtttaaaaag tcatctgtca agaaatatct ttaatcaaac tttataattt
      901 ctgaacttga agataataac tcaaaaaaca ctttttgtct ttggaaacaa ttgaagctta
      961 gtcacctatt gtatattttt ccttactttt tccagaaaag cctgcatcct ttctgtatct
     1021 ttcttttcaa taatgtccag caaagtagcc aattcagctt gaattttttt aatttgttca
     1081 ggtgtataag gattgaataa tatttcggtt aataaataat catcttctga cagcaaacct
     1141 tctgcaattt ccatatgccg tttaaaagta gttccaggcg cttcctgatg tttcatcaca
     1201 gcagcaaaaa ccaatgtgga agcaaaagga atagcaagtg aataagcagt cgttttatcg
     1261 tgctcttcaa aggaatattc aaaaattttc aaatgtaggg aatgatacaa atctctgaaa
     1321 aaagtttttc ccagaaaatc agactcagaa ataatgatgg cgttctggga actcaaatta
     1381 tccagtgttg caaaagtagg accaaacata ggatgtgtgg atacaaaacg tttacccgta
     1441 ctttcgtaaa attccttcaa ccctgttttt actgaagcaa tatcggaaag catgcaattt
     1501 tccggtagaa aaggcatcac attttgaaaa gcctcaatgg taaatttcaa tgtaacacaa
     1561 ttcagcacca gctctggctg aaattccctg atctgttcat aatcagtcag tcgataagta
     1621 ttgaaaacaa accgaagttt tttaggatca atatcgaata aagccacttc atgatggaga
     1681 cacaataaat ccgtaaaaaa agtgcccatt ttaccggcac caagtattaa gattttcatt
     1741 ttctaaaata aaattatatt ttctacaaaa gtagaaataa aattaaaaaa gaaaacacga
     1801 tacgtaagga aaattgttcc tgataaaatt attgaaatta agttgttgtc tttttcttcc
     1861 caaattcttt gtcaattttc ataaagggaa tgacaaccag cgttggaata gcacaaagta
     1921 ctacaaaaat aaagaaatga tgatagccta acaattcctg taaccagcca gcaatcatac
     1981 ctggcaacat catgcccaat gccataaatc ctgtacaaat agcataatga ggtgttttat
     2041 gttcaccttc cgagaaataa atcaagtaca acatataagc agtaaagcca aaaccatacc
     2101 caaattgttc aattgcgact gccaggttga ttaacaacat gttttcaggt tgtactgagg
     2161 aaagatataa atacaccaga tgaggtagcg tgatgcacaa agccattggc cagatccaga
     2221 atttcaatcc cttttgtgaa gcgacgatac cgccaataat gccaccaatt gtcaaagcaa
     2281 caacgccaac cgttccataa accagtccaa cctgaccggt agtcaatcct aaccctccaa
     2341 tttcgcgcgg atccagcaaa aaaggtgaag ccatttttac taacattgct tcgcctaaac
     2401 ggtaaagaag cataaaggca atagccagac cgattccctt tttctggaaa aaagtggcaa
     2461 aagttcttcc aaactcccgc aagatatctt ttgctgtatt tgttaaagta ggatggtctg
     2521 aatcaggata aggcaaaata aaacgatgat agaaataaat caagataaag aaccctgcaa
     2581 tgataaagaa agtgatgctc caagccaatt ttacatttcc tgaaactcct ttgaactcac
     2641 tttttacttc atctttcaat tttgggtcta actgaaatac tacataagcc ggtttatccc
     2701 agtttgtttc atcaaaaacc aatcgttcac ccgtgaccag cgaaatactt ttgtcacctt
     2761 ttgcatgggt tatattgaat atcaatgatt ttcctttttc tggctttttt gttaaacgga
     2821 tttgaaccaa gccagcatta cccactcttt ccgttaaatc agtttttatt tctcgttttt
     2881 ctccaaaatg gtctcgaata taatttgcaa gtggatcaga cacatttctt ttccaccagg
     2941 aatttttctc tgactttcct gtaactgttt tttgttcttt ttctacaaaa cgattcgtga
     3001 tattctgttc agttgcatat tgaagaattt tattcaacga atcccgactg atggtttgtg
     3061 tgctcaacac caactgagaa ggataagcca caaacgacaa ttctgctgta tcagcaggaa
     3121 gtacgttttc cggaatttcc atcatttcta tttgggaaga attagtagaa gcaactgtca
     3181 gtaaaacagg ttttgtccct gtatgacttt ccaatgcacc agccagaatg ataagcaacc
     3241 cttgcccggt aatcatggca aaacgataga aagtgcttcg tatccctaca aaaaaagatt
     3301 gttgagaact atccagtgcc agcatataaa agccatcggc tgcaatatcg tgggtagcag
     3361 aactgaaagc aataagccat aacaccgcta aggtggcttg aaaaaagaaa ggcacaggaa
     3421 tgagaaaagc aacgccagcc aatcccgcac caattaacaa ttgcatggaa agtatccacc
     3481 agcgtttcgt tttaataata tccacaaaag gactccaaaa aggtttgatc acccacggca
     3541 aatataacca gcttgtgtac aaggcaatat cggcatttga tatacctaaa cgtttgtaca
     3601 taatgaccgt aactgtcatc gccaccacat aaggaatgcc ttcagcaaaa tacaaagttg
     3661 gaacccaagc ccacggagaa acatttctgg tttgtttgtt tttcattgct tagtaatatt
     3721 tattaaaatt attttttctt ctatttaaca aacaaacttt tcattttgga aggttaaaat
     3781 gctgataatg aaaaaaatca aatggttcct tttctcaaat atacaacgat ttgttctcaa
     3841 caaatcagga atgtcaatta caatttttat aaaactgatt ctttataaat tgtgagacaa
     3901 atgtaaacaa tcgtcaccaa attcaaaaga aattcttcaa aaaaattaat tttttgcagc
     3961 aatttaataa gaagagcacg atattataaa attggttatc aatggtataa ggagaaaaaa
     4021 taaattgata taaaagaaaa attaaattcg taagaagcaa gagttaattc attaaatttt
     4081 gaactgtatt tttacttgaa ctcataaacc atcttccaca aaaacaagga tttttttagt
     4141 tatgagtttc cgagactgcg agttttgagt gaaaaagttt ttatgaaatg ttttgctgtg
     4201 attacaaatt ttgcgagtag aaaaagtccc gtcagggaca aaacattggt agaaaatagt
     4261 ttagcagaaa ggattttgaa gtgccgtcag gtacgaaatt ttagttacga gttacgagtt
     4321 tttaaatgta aagccaaaat atagaataaa tgacattagc cgatgagccg aaaaataagt
     4381 gaagttgacg ttaaaaaatc ttttctgttt gagcggcgaa gaagcgagtt taaaagattt
     4441 tagccaacaa acgcccattt ttccacctta tcttactaaa aaccttccta acagaaatga
     4501 ctttccccta aatcaagcct tattacctta ttaagaaaca gaataaaaat catttttaca
     4561 cttcattcat cttaataaat aataagctga taattaagta aatacttctt ccagcacttc
     4621 caacgattga atgttagaaa gaccagatat atgcagttga taatattgca aaacattttc
     4681 taacaattcc tttcgctttt gatgatttaa aagcacattt gccatagttg aataattact
     4741 tttcaataat aagataaaat ctactgttag ttcaggaccc aaaaagtgtg aatgaagagg
     4801 tggatgcgaa agaaaagtgc catttaacaa atcaaagtaa gttttagaag gattatcgct
     4861 attgggcttg aatccaagaa aacgagttaa ttgaagtaaa aatattaaat gaaaatttga
     4921 agttccttct tccattgtat ctaaaatttt tattgaattc tccaaaaacg aatacagttg
     4981 ttcatccgtt tctgcttgca aaagcacttt aaaaagaatt tcggaaagaa ataatgctat
     5041 tgaattttta actgggtcaa aaaagagggt ttgtggcaaa aaggaaagtt tgatatcttt
     5101 tatttgttga atttctttct ttggagaatg gcaaacatct aattctacaa ttgaaaatgg
     5161 ctgaaaaaag gcaggtttat agattgattt ctttttacct tttccgtaaa ccatataagc
     5221 atttcgtcca aattgacgag tataaatagt cacaatagaa gcaaaatcag aatatctggt
     5281 agaatgaagc actatacctt ctgttttggt cagcattttc aaaaaatttt tagcaacaca
     5341 aaattaatat attttctatc ctcgatcatc aattatttca aataaccttc attctttgta
     5401 atttttttct ttgctatttc tattcaactt agattatagg acattgattt acagtgcatt
     5461 ggaatttaaa atgtttattt atttgtttca tttgattgat aatactctcc aagtatttta
     5521 tttgcttttt caaaatcttt ttcaaaaata attaatttaa ttccttcatt aatattactc
     5581 aacatgggat aaagttcaac tgttttttca ttgttaataa aacattcgat
//
LOCUS       DAOTKS010000057         5624 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_13014_length_5624_cov_3.943257, whole genome shotgun sequence.
ACCESSION   DAOTKS010000057 DAOTKS010000000
VERSION     DAOTKS010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5624
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13014_length_5624_cov_3.943257"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="P5236_01940"
     CDS             <1..342
                     /locus_tag="P5236_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63154.1"
                     /translation="PHIAGYSIEGKANAAAQSVKAVSRFFGLGLDNWEPTDLPEPFPL
                     KTTSFESPMDFFLQTYNIEADSQQLKSFPEGFENQRSNYIFRREPKAYLNQLEEDFKE
                     KLTKQFEIFFR"
     gene            426..1829
                     /gene="dacB"
                     /locus_tag="P5236_01945"
     CDS             426..1829
                     /gene="dacB"
                     /locus_tag="P5236_01945"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HRU63155.1"
                     /translation="MKNLLYTILLCGIVTNSVAQLPVDLFVNDPLLKNANVGLVVKDL
                     KTNKILYSYQPNKCFVPASVLKLVTTATALELLGPDFRFQTQLEIDGNLSADGVLNGN
                     LFIRGSGDPTLGSVKLGDKNFLNKWIKAVQEKGIKKINGQVIADASLYDNEGVSPFWI
                     WEDIGNYYAAAVYGISYLDNTFQLVLQSGPVGTTPKIVRVIPPLPDLIIENYLKSTLI
                     GFDSAYFYGMPYSNIRRLYGEIPANRSSFVVKGDIPNPVLLLAQHFTEQLNKNGIPVT
                     QDATDQVPFSRNRTVIYTHYSPPLKEIIAEINKNSNNHYAEQLFRYLALQNEKVATNS
                     GAIKVITNFWKSKALDVNQLFLYDGSGLSPMNTVSAQFLTDLLTYMFTTSKNKTDFIH
                     SLAVSGDQGTLKSFLANTPLEGKVYAKSGTIARVKSYAGYIKQEDKTYAFVILVNNIR
                     NSKAVQHRIEKLLLSIQ"
     gene            2117..2953
                     /gene="dinD"
                     /locus_tag="P5236_01950"
     CDS             2117..2953
                     /gene="dinD"
                     /locus_tag="P5236_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006848896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HRU63156.1"
                     /translation="MKSDEIRNLFYQFETAALELEDIECWSARELQKLLGYSKWENFE
                     KVIQKAKDACKNAGEKVDYHFPDIRKTIAMPKGAEKEINDILLTRYACYLIAQNGDSR
                     KEEIAFAQNYFAVQTRRAELVEQRILEFERVKAREKLSQTEKQLSGILYERGVDNQGF
                     AIIRSKGDQALFQLNTKMLKRKMGIPDSRPVADFLPTISIKAKDLAAEMTGFNVQNKN
                     LKGQTNIEKEHVDNNTAVRNMLTKRGIVPENLPPAEDIKKLQRKLEGEEKKMLKNIKK
                     KK"
     gene            3302..4021
                     /locus_tag="P5236_01955"
     CDS             3302..4021
                     /locus_tag="P5236_01955"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRU63157.1"
                     /translation="MNNFLFFPETSSTNILLWQKLNEEKLPEGFVVYTDFQTAGKGQV
                     GNSWESAAGENILFSLLLYPQHIAIEEHFLISQITSIGIKKMLDNYISDVTIKWPNDI
                     YWKEKKLAGILVETSLQNRIIKSAVIGVGLNVNQKEFLSDAPNPVSLFQILGEKLDRQ
                     TLMNSIVQNILDIYYHWDNNKIRSCYQQFLYRKEDYHLFEANKEVFLAKIVDVLPSGQ
                     IELSTKEGVTKKFYFKEVKYL"
     gene            complement(4035..4250)
                     /locus_tag="P5236_01960"
     CDS             complement(4035..4250)
                     /locus_tag="P5236_01960"
                     /inference="COORDINATES: protein motif:HMM:NF027444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4834 family protein"
                     /protein_id="HRU63158.1"
                     /translation="MNLIFSILIFLLFFFLFGVIVLFGFFRSLFNFRGKGNKIFHHRK
                     REEKPIQRKEKIFDKNEGEYVDFEEIE"
     gene            complement(4261..5208)
                     /gene="metF"
                     /locus_tag="P5236_01965"
     CDS             complement(4261..5208)
                     /gene="metF"
                     /locus_tag="P5236_01965"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007764101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HRU63159.1"
                     /translation="MSVVEKIMNSPSTAFSFELLPPLKGDGIESVYKTIDLLREFDPQ
                     YINITTHRSEVVYKKNPQGLFEQVAIRYRPGTVAIAAAIQNKYKITVVPHVICSGYTK
                     EEIEYELIDLQFLGITDLLLLRGDKGKNDKEFIPTGHLHATDLQEQVNNFNKGFFLDG
                     TRMKSRVIPFSYGMACYPEMHEESPNFESELYYTKKKVDGGAEYLVTQMFFDNQKYYD
                     FVKKCREIGITVPIIPGIKPITLMNQLTVLPKIFHVEIPEEFATELRKCRTDKEATEV
                     GVEWCIKQAADLKAHGVPSIHFYSLMAAQSVKRVAKAVY"
BASE COUNT         2032 a          866 c          904 g         1822 t
ORIGIN      
        1 cctcatattg caggttattc catagaaggt aaagctaatg cggcggcaca aagtgtaaaa
       61 gcagtaagta gattttttgg attgggcttg gacaactggg aaccaactga tttgcctgaa
      121 ccttttccac ttaaaactac ttcttttgaa tctccaatgg attttttctt acaaacatat
      181 aatattgaag ctgatagtca gcaacttaaa tcatttcctg aaggttttga aaatcaacgt
      241 tccaattaca tttttcgccg cgaaccaaaa gcatacctga atcaactgga agaagatttt
      301 aaagaaaaac tcaccaaaca gtttgaaatt tttttcagat aaaataattt cagaattttt
      361 tctatatttg aattagtaaa gagctcttat ttgattttgt tctataagtt aaaagtaaat
      421 ttctgatgaa aaatctatta tacaccattt tattatgtgg aattgtcact aattcagtag
      481 cacaacttcc agtggattta ttcgtcaatg atccattgtt gaagaatgcc aatgtcggtt
      541 tagtcgtcaa agatttaaaa accaataaaa tactttatag ttatcagcct aataaatgtt
      601 ttgtacctgc atcagtgttg aaattggtta ctactgcaac cgcactggaa ttgttaggac
      661 ccgattttcg ttttcaaact caactggaaa ttgacggaaa tctgagtgct gatggtgttc
      721 tgaatggtaa cttatttatt cgaggaagtg gagatccaac attaggctca gttaagttag
      781 gcgataaaaa ctttctgaac aaatggataa aagcagtaca ggaaaaagga ataaaaaaaa
      841 ttaatggtca agttattgca gatgccagtt tgtatgacaa tgaaggtgta agtccctttt
      901 ggatatggga agatattgga aattattatg cagcagcagt ttatggaatt tcttacctcg
      961 acaatacttt tcaattggtt ttgcaatcag gacctgtagg aacaacccct aaaattgttc
     1021 gagtaattcc tcctttaccc gatttaatta ttgaaaatta tttaaaaagt actttaattg
     1081 gatttgacag tgcttatttt tacggcatgc cttattcaaa tatacgaaga ttatatggcg
     1141 aaattccagc caaccgttct tcttttgtag taaaaggcga tattcccaat cctgtcttat
     1201 tgcttgctca gcattttact gaacaactta ataaaaatgg aattcctgta acccaagatg
     1261 ctactgatca agtgcctttt tctcgtaaca gaacggtgat ttatactcat tattccccac
     1321 ctttaaagga aattattgct gaaatcaata aaaacagcaa caatcattat gcagaacaac
     1381 tttttcgtta tctggctttg caaaatgaaa aagtagcgac caatagcgga gcaataaaag
     1441 taattaccaa tttctggaaa tcaaaagctc tggatgtaaa ccaattgttt ttatatgacg
     1501 gttcaggtct ttcacctatg aatactgttt cagctcaatt tttgaccgac ttgttgactt
     1561 acatgtttac caccagcaaa aacaaaacag atttcattca ttctcttgca gtatcaggtg
     1621 atcaaggaac attgaaatct tttcttgcca acactccgct tgaagggaaa gtgtatgcta
     1681 aaagtggtac tattgcccgt gtaaaatcgt atgcaggata tataaaacaa gaagataaaa
     1741 cttatgcttt tgtcattctg gtaaataata taagaaactc aaaagcagtg cagcatcgaa
     1801 ttgaaaaact gttattatca attcaataag cgggaaaaat ctgtcagtat gttttaaaaa
     1861 attgaccttg tctgatgagc cgaaaaatga atagttgcga ggtgcattga gcaaataaaa
     1921 atttcctttc ttacattaaa aaaattccct cccctatagt ctcttatatt ttagattttt
     1981 tctttacctt tgaagtgcca ccttaattag gtaatgggaa atgcaatgag attttacttg
     2041 aaaatggttc atgtatgttt ttcataatta aggatgattt tcaaataata aattgattca
     2101 taaaaaatat ttcaaaatga aatccgacga aattagaaat ttgttttatc aatttgaaac
     2161 tgcagcttta gaattggagg atattgaatg ttggagtgca cgagaattac aaaaattatt
     2221 gggatatagc aaatgggaaa attttgaaaa agtaatacaa aaagctaaag atgcttgcaa
     2281 gaatgcagga gagaaagtcg attatcattt tcctgacatc aggaaaacga tagcaatgcc
     2341 taaaggtgct gaaaaagaaa taaatgatat tcttttaaca agatatgctt gctatttaat
     2401 tgcacaaaat ggtgatagca gaaaagaaga aattgcattt gctcaaaact attttgcagt
     2461 acaaactcgt cgagcagaat tagttgagca aaggatttta gaatttgaac gagttaaagc
     2521 gcgtgagaaa ctaagtcaaa cagaaaaaca actttctggt atcctttatg aacgaggagt
     2581 tgataatcaa ggatttgcta taataagaag caagggtgat caagctctat ttcagttaaa
     2641 tacaaaaatg cttaaacgta aaatgggaat accggatagc agacctgtgg ctgatttcct
     2701 tcccacaatc agcattaagg ctaaagatct tgctgctgaa atgactgggt ttaatgttca
     2761 aaataaaaat ttaaaaggac aaacaaatat cgaaaaagaa catgttgaca acaacacggc
     2821 tgttcggaat atgttaacaa aaagaggaat agttccagaa aatcttccac cagcagaaga
     2881 tataaaaaaa ctgcaacgaa aacttgaagg tgaagagaaa aaaatgttga aaaacattaa
     2941 aaagaaaaaa tgataaaagt tatttgaaca acactattta ccaaatatta taagcaaata
     3001 cagatttgaa tatgtttatt atccatttct ttgttaaaaa agatttctta caagctgatt
     3061 tcagaagtac tttttttacg cgaaaacaat ggcaaactgt tgttaaaaaa cattctgtca
     3121 caaataattt acaatctttc gttgttttat taacggattt gcttctacaa aacaataaaa
     3181 tattcagatt caaacccaat aaaaatagtt ctttctgtta agatattttg agtaaattct
     3241 cactgaacaa tttgaatatc aatgattatc aatgattatc aataataaaa taactaaagg
     3301 aatgaataat tttttatttt ttcctgaaac ttcttctacc aacatattat tatggcaaaa
     3361 gttgaacgaa gaaaagctgc ccgaaggttt tgtcgtttac actgattttc aaactgctgg
     3421 caaaggacaa gtaggcaatt cttgggaatc agcagcaggc gagaacattc tttttagttt
     3481 acttttgtat cctcaacata ttgctatcga agaacatttt ttaatttcac aaatcaccag
     3541 cattggcata aaaaaaatgt tagataatta tatttctgat gttaccatta aatggccaaa
     3601 tgatatttat tggaaagaaa aaaaactggc cggtattctc gtggaaacat ctttacagaa
     3661 tagaataatt aaatcagcag tgattggagt cggtctgaat gtgaaccaaa aagaatttct
     3721 gagcgacgca ccaaatcctg tgtcgctttt tcaaatttta ggcgaaaaac tggacaggca
     3781 aacattgatg aattcaattg ttcagaacat tctcgatatt tactatcatt gggataataa
     3841 taaaattcgt tcttgttacc aacaattttt atatcgaaaa gaagattatc atttatttga
     3901 agctaataaa gaggtttttt tagcaaaaat tgtagatgtt ttaccaagtg gacaaattga
     3961 attatcgacc aaagaaggag taacaaaaaa attttatttc aaggaagtca aatatttata
     4021 atcaactttg atttttattc tatttcttcg aaatcaacgt attcgccttc gtttttatca
     4081 aaaatttttt ctttgcgttg gataggtttc tcttctcttt tgcgatggtg aaatatttta
     4141 tttcctttcc ccctgaaatt gaaaagagaa cgaaaaaaac caaataaaac gataacgcca
     4201 aataaaaaga aaaacaataa aaagattaat attgagaaaa ttaaattcat gtcaattttt
     4261 ttaataaaca gccttagcta cacgtttcac gctttgtgca gccattaagc tataaaaatg
     4321 aatacttggc acgccatgtg cttttaaatc agcagcttgc tttatacacc attctacacc
     4381 tacttcagtg gcttctttgt cagtgcggca tttgcgtaat tctgtagcaa attcttccgg
     4441 aatttctaca tgaaaaattt taggaagtac tgttaattga ttcattagtg taatgggttt
     4501 aattcctgga ataatgggaa ccgtgattcc tatttcacga cattttttta caaaatcata
     4561 atacttttga ttatcaaaaa acatttgggt taccaaatat tctgcacccc catcgacttt
     4621 tttctttgta taatacaatt ctgattcaaa atttggagat tcttcgtgca tttctggata
     4681 acaagccatt ccataagaga aaggtatcac acgtgatttc atgcgagtac catctaaaaa
     4741 aaatccttta ttaaagttgt ttacttgttc ttgtaaatcg gtagcatgca gatgacctgt
     4801 cggaataaat tccttgtcgt tttttccttt atcgccacgt aaaagcagta aatcagtgat
     4861 tcctaaaaat tgcaaatcta tcagttcata ttcaatttct tctttggtat aaccactgca
     4921 aataacatga ggtacaacgg tgattttata tttattttga atagcagccg caatggctac
     4981 cgtaccagga cgatatcgaa tggctacttg ttcaaaaagt ccttgcggat tttttttata
     5041 tactacttca ctgcgatgag tagtaatatt aatatattgg ggatcaaatt ctctcaataa
     5101 atcaatggtt ttataaaccg attcaatacc gtcacctttc aaaggtggga gcaactcaaa
     5161 agaaaaagcc gttgaaggag aattcattat tttttcaaca acactcataa attttgataa
     5221 ttacaataaa atgcttcttc gcaaattttt acttacaaaa gtacaaattt aattttgttc
     5281 taaaaatgaa ctttgaataa gatatgcaaa acaatgtgaa acatagaaga ataactattt
     5341 gaaaaaattt atatttcgta atacaaacca attgaaaata aaatatcgaa ataaataaat
     5401 tcgaagcaaa tgttgcaaga ttatgttaaa aatagatttt tcttcatttg tgccaaacca
     5461 aaaaaatagc aaaatgtaat aacaacaaag catgttagta agtatttttt cgcctatctg
     5521 caaattaatt gaaatttatc atttatattt gttagtcagt tgtgtaaact cgtaaattca
     5581 agtaattaat gcaacttttt caggggaaat aaaaggtaaa gaaa
//
LOCUS       DAOTKS010000058         5618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_13038_length_5618_cov_3.385583, whole genome shotgun sequence.
ACCESSION   DAOTKS010000058 DAOTKS010000000
VERSION     DAOTKS010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13038_length_5618_cov_3.385583"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3198)
                     /locus_tag="P5236_01970"
     CDS             complement(<1..3198)
                     /locus_tag="P5236_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRU63160.1"
                     /translation="MKKNHHEKRGGNILEEYFSQTMRIIRVGLFFIVVSTTISWNTTS
                     YSQSTSLSLDMKDVTVGEVIKAIENQSEFLFLYQGGQVDLNRRVSIQIDRKQVQEILK
                     ELFKGTDNIYTVDNRKIIINKASRKILDNHLAATEKNLRMDLQQPQQREITGKVTDVS
                     GEPLPGASVMVKGTNIGTITDKEGRFSLRIPSNAQILQISFVGMKSQEIPIEGRTMFN
                     VVLEEETTLLQEVVAVGYGTMTKRDLSASVSSIKSDKIEERTYAFNIMESMAGKLPGV
                     RNVSFSGKPGGASSLRIRGMGSINADSNPIYVLDGVVGVDPSIINPTDIESIDVLKDA
                     AATAMYGAKGANGVVLITTKSGKKDRGTFSYNGKIGVGLLTRKIDVLNAEEYMEVQRR
                     AYAYSGKVMPHLQTPMEKLFYYAKDASGNYLRDENGYLIASPKYDTDWQDAITRNAPI
                     NDHNLTFTGGSEKSTIYANIGYQDFEGLVKYTYSKRLTGTLNIKSDIKEWLNLQALIT
                     TGFNKINDMEGGFGQGPVRNMIEMPPIVPVKYEDGTWGRKNDYPLGEAGEDPILLLKE
                     QKNIWDGNFTILNLISTFKFTNNFHFTAKGDLQTSNKRHITYAKAGLLDVSETNNGYA
                     DIYNEVSKKWSNEDYFSYHNNFFNNQLVSDFVLGASWYYYRLENSSGGSENYFDDSFE
                     YYNLGAGTTFHKPSSGMDQNTMNSFYFRMNHSFRDKYLLGLTLRADGASNFGENNKYG
                     FFPSVSGAWRISEENFFESAKPIINNLKLRLSYGLVGNASIPNYKTISSYSNSSVIFN
                     KSLQPYVVLSNLGNEDLKWETSKQFNAGMDIGFLNNRIEILMDYYNKATTNLLFLKQV
                     PYTTGYSNTWTNLGEIANRGFELTLSSRNIANKNFVWSTDIIFSTNKLLVIDIGGETI
                     DTGNGTVAKEGEEWASYFILNRIGTWGLNEVAEAAVYGKKPGDLKYEDVNKDGIINDD
                     DRKLMGNGMPKGEINMGNTFRYKGFSLMLDLNCQYGAKILGITTTMLENRQLYGNSRK
                     TVLDAWTPEHQNTMIAAIRLPSDPNFGENEKDSRML"
     gene            4683..5411
                     /locus_tag="P5236_01975"
     CDS             4683..5411
                     /locus_tag="P5236_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRU63161.1"
                     /translation="MKIILDHNSPIPLYAQIEDQIREAIKSPEYKNGKMLPSEVDLSK
                     QLGISRSTLRHAINKLVFEGLLVRKKGIGTFVANKAVSTKLSNWLSFTQEMRALGIEV
                     KNYELHVQWEKPNEELCNFFNISPNTKILRLERLRGKPEYPFVLFISYFNPRIGLTGN
                     EDFSRQLYDILENDYNTIVKYSKEEISAKIADKKLAEKLQINVGDPILVRKRHVYDPG
                     NHPVEWNIGYYRADSFVYTIEAER"
BASE COUNT         1863 a          959 c          733 g         2063 t
ORIGIN      
        1 caacatccga ctatcttttt cattttctcc aaaattagga tctgaaggta aacgaatcgc
       61 agcaatcata gtattttgat gctcaggcgt ccatgcatct aaaactgttt tcctagaatt
      121 tccatataat tgcctgtttt ccaacattgt cgtggttatt ccaaggattt tagcaccata
      181 ttgacaattc agatcgagca ttaatgagaa acctttataa cgaaaagtat ttcccatatt
      241 tatctctcct tttggcatgc cattacccat taatttacga tcatcatcat ttattatacc
      301 gtctttatta acatcttcat actttaaatc tcctggtttt ttcccgtaga ctgctgcttc
      361 tgcgacttcg ttaagacccc atgttcctat tctatttaat ataaaataag aagcccactc
      421 ctcgccttct tttgctactg ttccattgcc cgtatcaatt gtttctcccc ctatatctat
      481 aactaataat ttattagtcg aaaaaataat atctgttgac catacaaaat ttttatttgc
      541 aatatttcgg gaagacaacg ttaactcaaa tcctctatta gcaatttctc ctaaattagt
      601 ccacgtattc gagtaacctg tagtataagg aacttgcttt aagaataata aatttgtcgt
      661 tgctttgtta taataatcca ttaatatttc aattctatta tttaaaaacc ctatgtccat
      721 gcctgcatta aactgttttg atgtttccca ctttaagtct tcattcccca agttactaag
      781 aacaacataa ggttgtaaac tcttattgaa tatcactgaa ctattagaat aagaagaaat
      841 tgttttataa ttaggaatag aagcatttcc taccaatcca tagcttaatc ttaatttcaa
      901 attattaatg ataggtttag cagattcaaa gaagttttct tcagagatac gccaagcacc
      961 tgacactgat gggaaaaaac catatttatt attttctcca aaattagagg ctccatctgc
     1021 tcttaaggtt aagccaagca aatacttatc acgaaaagaa tgattcattc taaaataaaa
     1081 agaattcatt gtattttgat ccatcccaga agacggctta tgaaaagtag ttcctgctcc
     1141 taaattataa tactcaaaag aatcatcaaa ataattttca gagcctccac tagaattttc
     1201 aaggcgataa taataccaac ttgctcctaa aacaaaatca gataccaatt gattattaaa
     1261 aaaattattg tgataagaga aataatcttc atttgaccat tttttactta cttcattata
     1321 aatatcagca taaccattat ttgtttcgct tacatctaat aatcctgctt ttgcatacgt
     1381 tatgtgtctt ttattagaag tttgtaaatc ccctttggct gtaaaatgaa aattattagt
     1441 aaatttaaaa gtggatatta agttcaatat tgtgaaattt ccatcccaaa tgtttttttg
     1501 ctcttttaat aaaagaatag ggtcctcacc agcttctcct aagggataat catttttacg
     1561 accccatgtc ccatcttcat actttacggg aacaatagga ggcatttcaa tcatatttcg
     1621 tacaggacct tgaccaaatc ccccttccat gtcattaatt ttattaaatc ccgtcgtaat
     1681 taaagcctgt aaatttagcc attcttttat atcacttttt atattaaggg tgccggtcaa
     1741 tcgtttcgaa taagtatatt ttactaatcc ttcaaaatct tgatagccta tatttgcata
     1801 aatagtagat ttctcacttc ctccagtaaa agtaagatta tgatcattaa taggtgcatt
     1861 tcgggtaata gcatcttgcc aatcagtatc atactttgga gaagcaatta aataaccatt
     1921 ttcatctctt aaataatttc ctgaagcatc tttggcataa taaaaaagtt tttccattgg
     1981 tgtctgaaga tgaggcatca ctttgccact ataggcatat gcgcgtcttt gaacctccat
     2041 atattcctca gcatttaaga catctatttt ccgtgttagg agtcccacac caattttacc
     2101 attataacta aaagtaccac ggtctttctt gccactttta gtagtaataa ggactactcc
     2161 attagcacct tttgctccat acatggcagt cgcagcagca tcttttaaga catcgataga
     2221 ctctatatct gtgggattta taattgaggg atcaacccct actaccccat ccagaacata
     2281 aatcggatta ctatctgcat taatagatcc catgcctcga attcttaacg aagaagctcc
     2341 ccctggtttc cctgaaaagc taacatttct cacacctgga agttttcctg ccatgctttc
     2401 cataatattg aatgcataag tcctttcttc tattttatca cttttaatag aactaacaga
     2461 ggcactgagg tctcgctttg tcattgtccc ataacctact gctacaactt cttgtaaaag
     2521 tgtagtttct tcttccaaca ccacattaaa cattgttcta ccttctatgg gaatttcttg
     2581 acttttcatt cccacaaatg aaatttgtaa aatttgtgca ttagaaggaa ttcgaagaga
     2641 aaatcttcct tccttatcag tgattgttcc tatattagta ccttttacca tcacactggc
     2701 accaggcaaa ggctcacctg aaacatcggt tacttttcct gttatttccc tttgctgagg
     2761 ttgttgtaaa tccattctta aatttttttc agtagctgct aagtgattat ccaggatttt
     2821 tctggatgct ttattaatta taattttacg attatccaca gtatagatat tatctgtccc
     2881 tttgaacaat tcttttaata tctcttgaac ttgcttcctg tcaatctgaa tagacactcg
     2941 ccgattcaaa tctacttgtc ctccttgata tagaaacaaa aactcgcttt gattttctat
     3001 cgctttaatt acttctccaa cagtaacatc tttcatatcc aacgaaagac tcgtgctttg
     3061 cgaataagaa gtggtattcc aactaatcgt tgtactcacc actataaaaa ataaacctac
     3121 tcgtataatt cgcatcgttt gagagaaata ttcttccaaa atattccccc cacgtttttc
     3181 atgatgattt tttttcatac tttctttttt ctttttggtt atttttaaat aaatattttt
     3241 ttaaaagcaa agattgacac ttttttattt ttgtttagtg tttttggcta atctcattgt
     3301 ttttttattc cataggcatt attctttttt aaggtttaac aatggaaaga gggaatttga
     3361 aaaaatctta aaaatccaat atccgattta ttcaaattct tctcaaaaag aagattattc
     3421 tctctaaaaa agtaaaatta tttctgtatt tctattatca ctaaactatt atcctaatta
     3481 aatcttatct atttacatat caattattta cagataatat acttaaattt attcttatcc
     3541 aaaaagatta tatgtcaaag atatttgctc tgttattcac gttgtattaa aatttttctt
     3601 tcactgtttt agttcgcttt gtgaatccac tagaagataa aacatcaacc acatatataa
     3661 ttaaaataag tttttattgc ataaaaaaat taataatttt atctgaagaa cgattttata
     3721 agttaaaacc attaaaatat gaattaaaat atgaattaag atttaactct tcccataata
     3781 tcctttaaat tactataaag ttaagagtta ttggatatct gaaattagct atctctcctc
     3841 gaggagccta attttatttt caaagaataa ttcgcagtat aggaatctgt ccttaataag
     3901 tcaagctcat tttatttctc taatagcatg gttaaagata actctttgaa aaaatctttc
     3961 tttgttcatt ttttgtcgtc agtcaaaggt ttacttttgt gttaactaaa agtactaaaa
     4021 ggttattttt accttatgag atttttcagg gtaacaagtt ttttgcaaat ttttatcttg
     4081 tagtagttga atagattaga acatctaaaa aattacaaaa agtttcactc cctcttttag
     4141 acagtattca ttttttccac atagcttgtc aagacgtttc acgctggtca aaagaatatc
     4201 ccacaataaa tgaatgatct tgtttgcttt aggttaagtt ataccaaaaa agctgtctct
     4261 tgttcttgtg agattacgtt tttagataaa tcaaaatgaa cattctaact ataaaaattg
     4321 agttttgttc ttttaatgga gtatatcaac atattgtttt tttatcttta aattgagaaa
     4381 aaaacagcgg tgtttgaatt atcagtctct taatcaacca agtaataatg tattttttta
     4441 attcatttat attacttata tttgcttttg aactatgttc gtatgtttat atgtctatac
     4501 atataaattc actacaaatg taatagaaaa tttactgaat gcaaataatt ttagtttaat
     4561 taacacagat taaaaatcaa acatattctt aaaatttaag ctactcttaa agtaaagctt
     4621 ttaacaaaaa aatttattta aattaaaaat aataattaaa tttgcaattt aattcattga
     4681 ctatgaaaat aatattggat cataatagcc ctataccttt atatgctcaa attgaagacc
     4741 aaattcggga agctattaaa tcacccgaat ataaaaatgg aaaaatgctc ccaagtgaag
     4801 ttgatttgtc taaacaatta ggcatttcac gaagtaccct tagacatgct attaataaac
     4861 tagtttttga aggtttgttg gtgaggaaaa aaggaatagg tacttttgtg gcaaataaag
     4921 ctgtaagcac taaattaagt aactggctaa gttttacaca agaaatgcga gcattaggta
     4981 tagaagttaa aaattatgag ctccatgttc agtgggaaaa acctaatgaa gaattatgca
     5041 atttttttaa tatctctcct aatactaaaa ttttgagatt ggaacgatta agaggaaaac
     5101 cagaatatcc ttttgtactt tttatttctt attttaatcc acgaattggt ttaactggta
     5161 atgaagattt ttctcgtcaa ctgtatgata ttttagaaaa tgattacaat acgattgtta
     5221 agtattcaaa agaagaaata agtgcaaaaa ttgctgataa aaaattagca gaaaagctac
     5281 aaatcaatgt aggtgatcct atccttgtac gtaaacgaca tgtttacgat cccggaaatc
     5341 atcctgtgga atggaatatt ggatattata gagcggatag tttcgtttat acaatagagg
     5401 ctgaaaggta ataataaatt taagttaaat cctttccgaa aaacaatttt aggatctctt
     5461 gattcgtaag tcgttgaata ttaatttttt aattttagaa agagatgagt gtataaatta
     5521 taaaaatatt ccagcaaaat attttttatt taattagtta cttaaatcct cttcttatat
     5581 gatgaaaaat ttttttgtaa agctattatc ggtattta
//
LOCUS       DAOTKS010000059         5614 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_13057_length_5614_cov_3.959705, whole genome shotgun sequence.
ACCESSION   DAOTKS010000059 DAOTKS010000000
VERSION     DAOTKS010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13057_length_5614_cov_3.959705"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="P5236_01980"
     CDS             <1..829
                     /locus_tag="P5236_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HRU63162.1"
                     /translation="NGWDDPRMPTLCGLRRRGYTPEAIRNFIDKIGYTKFDALNDIGL
                     LEYSARENLNKIATRVSAVLYPVKLIITNYPDNEIEILQTPNNPEDPSAGTREIIFSK
                     ELYIEQDDFMENAPKNYFRLTPGQEVRLKSAYIVKCTGCKKNEKGEIVEVYCEYDPNT
                     KSGMPESNRKVKGTIHWVSVAHSLSAEVRLYDRLFRVENPSEEEGDFRELLNPDSLKI
                     LTGCKVETFLQYAKPLDHFQFQRIGYFTVDMDATPENLIFNRTVSLKDSWAKEQKKK"
     gene            999..1727
                     /locus_tag="P5236_01985"
     CDS             999..1727
                     /locus_tag="P5236_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HRU63163.1"
                     /translation="METWTDRTELLIGEKGLKILQDSSVLIVGIGGVGGMATEMICRA
                     GIGKITLIDGDIVNASNINRQIIALTSTLYRPKVEVMAERLSQINPELKLIIHHKWLD
                     FSNTISMLEENHFDFVIDAIDTLMSKVFLIKSCVERNIPIISSMGAGGKLNPSKVMIK
                     DISETTMCSLARAVRQQLAKIGIKKGVPVVYSTEPVIKKSIIPVANEANKKSTIGSIS
                     YMPAVFGCYLTSFVIRELLNKSRE"
     gene            1756..4572
                     /locus_tag="P5236_01990"
     CDS             1756..4572
                     /locus_tag="P5236_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HRU63164.1"
                     /translation="MSDEEIFEEENENILSTDEEELNSESFDTGHSTYQVPKITDESV
                     KHHLSGMFQNWFLDYASYVILERAVPDIHDGLKPVQRRILHSMKEMDDGRYNKVANIV
                     GQTMQYHPHGDASITEALVQLGQKDLLIDCQGNWGNILTGDAAAAPRYIEARLSKFAL
                     ETVFNPKITEWKPSYDGRKKEPVTLPVKFPLLLALGCEGIAVGLNSKILPHNFNELLD
                     ACIAYLKGEEFVLYPDFQTGGLIDISRYNDGERGGVVKIRAKINKIDNKTLAITEIPY
                     STNTSKLIDSILKANDKGKIKIRKIDDNTSSNVEILVYLQPGTSSDKTIDALYAFTDC
                     EISISPNCCVIHDKKPRFMSVSEMLCVSCEDTKNLLEAELKIRKKELEEQFFFISLEK
                     IFIENRIYKEKGFEDSTSHEVAIQYIDKQLEPFKANLIREVTREDILKLLEIKMVRIT
                     KFDADKANNTLIEIQKKIEETDYQLSHLIDYTIAWYESLKAKYGKKYPRRTEIRNFET
                     IEAAKVIEANEKLYVNREEGFIGTSLKKDEFVCNCSEIDDIIVFFKDGKYKVVKVAEK
                     MFVGKNILYINVFKKNDKRTVYNVVYRDGKTGPYYIKRFAVNSITRDKEYDLTQGKPG
                     SKIVYFSANPNAEAEIIRVTLKPKPRLQKLVFEKDFSEIAIKGRQTRGSILTKNEIHK
                     ITLKQKGGSTLGGREVWFDHDVLRLNYDKRGEYLGEFQNDDLILVITQQGEYYTTDFD
                     VNNHYENNILRLEKFEEDKIWSAAIFDADQNHYYLKRFQLSPSQKKVSFIGENPESRL
                     ILLSDKDFPRFEVIFGGKDAYRKPLVIEVSEFISAKSYEAKRKRITTHEIEIINELEP
                     LHTKQEIDTEQVSDYIEIEENGNDRNEESEILEKNFTSLEEASEIEKEESTMIFSEKD
                     SILPDELVNDKKNHDETSQQLSLF"
     gene            complement(4667..4849)
                     /locus_tag="P5236_01995"
     CDS             complement(4667..4849)
                     /locus_tag="P5236_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HRU63165.1"
                     /translation="MKDILNFLGAIIVLIGVCILGVYHFAGINSNTLLAIAGLLMIIG
                     FFTFLFMNKKSLKKDF"
     gene            5360..5536
                     /locus_tag="P5236_02000"
     CDS             5360..5536
                     /locus_tag="P5236_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63166.1"
                     /translation="MGLYMEIFNLSVVLPQLVASFKMGEVINMAANKSIIFAICTITL
                     TISGLLWFFVKNEK"
BASE COUNT         2028 a          822 c         1039 g         1725 t
ORIGIN      
        1 gaacggttgg gacgatcctc gcatgccaac tctttgtgga cttagaagaa gaggttacac
       61 gcccgaagct atacgtaact ttatagataa aattggctac accaaattcg atgcattgaa
      121 tgatattggt ttgctggaat attcagcacg tgaaaatctc aataaaattg ccacacgagt
      181 tagtgcggtg ctttatccgg tgaaattgat tattacaaat tatcctgata atgaaataga
      241 aatacttcaa acacccaata atcccgaaga tccttcggca gggacacgag aaattatttt
      301 ctcaaaagaa ttgtatatcg agcaagatga ttttatggag aatgcaccga aaaattattt
      361 tcgcctaact cccggtcagg aagtcagatt aaaaagtgcc tatattgtta aatgtacggg
      421 ctgcaaaaaa aacgagaaag gtgaaattgt tgaagtttat tgcgaatatg atccaaacac
      481 aaaaagtgga atgcctgaga gcaatcgcaa agttaaaggt actattcatt gggtttcagt
      541 tgctcattca ttatccgcag aagttcgttt atatgatcgt ttgttccgag ttgaaaatcc
      601 ttcggaagaa gaaggtgatt tccgtgaatt gctcaatcca gattctttaa aaattctgac
      661 cggctgtaaa gtggaaactt ttttgcagta tgctaaacct ttggaccatt ttcaatttca
      721 acgaattggt tattttactg tagatatgga tgctacacct gaaaatttaa tctttaatcg
      781 taccgtttca ctcaaagata gttgggcaaa agaacaaaag aaaaaatgaa ttttctttta
      841 caagtattgt gataaaaaaa ctgttgcatg tagttgtgac agttttttat ttatgaattc
      901 ctcgatataa aataaaaaat gataaagctt tttaaaaaat atggaattgt agttttaaac
      961 tgataacttt gtataaagaa aagttaagca atcattgtat ggaaacgtgg acagatcgca
     1021 ccgaattatt aattggagaa aaaggattga aaattttaca agattcttca gtcctgattg
     1081 taggaatagg aggggtggga gggatggcaa cagaaatgat ttgtcgtgca ggtattggaa
     1141 aaataaccct cattgatggt gacatagtaa atgcgagtaa tatcaatcgc caaattattg
     1201 ctttaacatc tactctttat cgcccaaaag tagaagtcat ggcagaacga ctctcgcaaa
     1261 ttaatcctga gttgaagttg attattcacc ataaatggct ggacttttcc aatacgatat
     1321 caatgttgga agaaaatcat tttgattttg tgatagatgc cattgacacg ttaatgtcca
     1381 aagttttttt gataaaatca tgtgtggaac gaaacattcc tatcatttct tctatgggtg
     1441 caggaggaaa attaaatcca tcaaaagtaa tgataaaaga tatttcggaa accaccatgt
     1501 gcagtctggc aagagctgtg cgtcaacaat tggcaaaaat aggtattaaa aaaggggttc
     1561 cggtagttta ttcaaccgaa ccagtgataa aaaaatccat tattcctgtt gcaaatgaag
     1621 caaacaaaaa atcaactatt gggagtattt catatatgcc tgcggtattt ggatgttatt
     1681 tgacttcctt tgtgattcgg gaattattaa ataagtcaag agaataattt attagacaag
     1741 atattataaa taaagatgag cgacgaagaa atatttgagg aagaaaatga aaatatatta
     1801 tcaactgacg aggaagaatt aaattccgaa tcttttgata cagggcattc aacttatcaa
     1861 gttcccaaaa taacagatga aagtgtgaaa catcatcttt caggaatgtt tcaaaactgg
     1921 tttctggatt atgcttctta tgtcattctg gagcgagctg tgcctgatat tcatgatggt
     1981 ttgaagcctg ttcagcgacg tattcttcat tctatgaagg aaatggacga tggacgctac
     2041 aataaagttg ctaatattgt tggtcaaacc atgcaatacc atccgcacgg tgatgcttcc
     2101 attacggaag cgctggttca attaggacaa aaagatttgt tgattgactg tcagggaaac
     2161 tggggaaaca ttttaacagg tgatgcagca gccgctccac gatatatcga agcacgtctc
     2221 tcaaaatttg ctctcgaaac cgtctttaat ccaaaaatca ctgaatggaa accttcgtat
     2281 gacggacgaa agaaagagcc agtaacttta cctgttaaat ttccattact tttagcttta
     2341 ggatgtgaag gaattgctgt tggattaaac tctaaaattt tacctcataa tttcaacgaa
     2401 cttcttgatg cttgtattgc ttatttaaaa ggagaagaat ttgttcttta tcctgatttt
     2461 caaacaggtg gtctgataga tataagtcgt tataatgatg gagaaagagg cggagttgtc
     2521 aaaattagag ccaaaattaa taaaatagat aacaaaacat tggctatcac tgaaattcct
     2581 tatagcacaa acacttccaa acttattgat tctattctga aagcaaatga caaaggaaaa
     2641 attaaaatca gaaaaataga tgacaatact tcttctaatg ttgaaatttt ggtttatctc
     2701 cagccaggta cttcgtctga caaaaccatt gatgctctgt atgcctttac cgattgtgaa
     2761 ataagtattt cgccaaattg ttgcgtcatt cacgataaaa agcctcgatt catgagcgtc
     2821 agcgaaatgc tttgcgtgag ttgcgaggat acaaaaaatc ttttggaagc agaattgaaa
     2881 attcgtaaaa aagaactaga agaacaattc tttttcattt cactggaaaa aatttttatt
     2941 gaaaatcgta tctataagga aaaaggtttt gaagacagta cctctcatga agttgctatt
     3001 cagtacattg ataaacaact cgaaccgttt aaagccaatc tgattcgtga agttacccga
     3061 gaagacatct tgaagctact ggaaataaaa atggtacgaa ttaccaaatt tgatgccgac
     3121 aaagccaata atactttaat agaaattcag aaaaaaattg aggaaactga ttatcaactt
     3181 tctcatttga tagattatac tattgcctgg tacgaaagtt taaaggcaaa atacgggaaa
     3241 aaatatccac gccgaacaga aattcgcaat tttgaaacca ttgaagctgc aaaagttatc
     3301 gaagccaacg aaaaacttta tgtcaatcga gaagaaggtt ttattggaac ttcgttgaag
     3361 aaagatgaat ttgtatgcaa ttgttccgaa atagatgaca tcattgtctt ttttaaagat
     3421 ggtaaatata aagtggtgaa agtagctgaa aaaatgtttg ttgggaaaaa tattctctat
     3481 atcaatgtct ttaaaaagaa tgataaacgc acggtttata atgtagtata tcgcgacggg
     3541 aaaacaggac cttattacat caaacgtttt gcagtgaata gcatcactcg cgataaagaa
     3601 tatgatctta ctcagggaaa gccaggttca aaaattgttt atttttcagc caaccccaat
     3661 gctgaagcag aaataatacg cgtcacttta aaaccaaaac cgcgtttaca gaaattggtt
     3721 tttgaaaagg attttagtga aattgctatt aaaggtcgtc agacaagagg gagcatttta
     3781 accaaaaacg aaattcataa aatcaccttg aaacaaaaag gaggttctac cttaggtggc
     3841 agagaagttt ggtttgatca tgatgtgttg cgtctgaatt atgacaaacg aggcgaatat
     3901 ctgggagaat ttcaaaatga tgatcttatc ttagttatta ctcaacaagg ggaatattat
     3961 actactgatt ttgacgtcaa taatcattat gaaaacaata ttttaagact tgaaaagttc
     4021 gaggaagata aaatctggtc tgctgctatt tttgatgctg accagaacca ttattatttg
     4081 aaacgtttcc aattatcacc atcgcaaaag aaagtaagct ttattggcga aaatcctgag
     4141 tcgcgtttaa ttcttctttc tgataaggat tttccacgct ttgaggtaat ttttggtggc
     4201 aaagatgctt atcgaaaacc tttggtgatc gaagtcagtg aatttatttc ggcgaaaagc
     4261 tacgaggcaa aacggaaacg tattactacc catgaaattg aaatcatcaa tgagttagaa
     4321 cccttgcata caaaacaaga aattgataca gaacaggtaa gcgattatat tgaaatagaa
     4381 gaaaacggaa atgatagaaa cgaagagtca gaaattttag aaaaaaattt cacatcatta
     4441 gaagaggctt cagaaataga aaaagaagaa tcaacaatga ttttttctga aaaagattct
     4501 attttaccag atgaattggt aaatgataaa aaaaatcatg atgaaacctc acagcaatta
     4561 tctttgtttt aataataaag atagaaacat tctatcatga aatgtcccta tctttatttc
     4621 tcaaaatttg gttataaaaa aaattaaaaa taaaaaacca acgtacttaa aaatcttttt
     4681 taagagattt tttattcatg aaaagaaaag taaaaaaacc tattatcatt aataatccag
     4741 caattgctaa caaagtattt gagttaatac cagcaaaatg atatactcct aatatacaaa
     4801 cacctattaa aacgataata gcacccaaaa aatttaaaat atctttcatc ttctgaaaaa
     4861 gtttatttgt ccttttattg gcacaaatta aataaatatt tatcaagtaa tcaaatattt
     4921 tttacaaaat taggctgaat gttccaaaaa tttttaaata cttagaaaac aattcttttt
     4981 atatcatttt ctgtttttaa taagctaata aggttggtat gattcgttta tcatttgtta
     5041 ttaaggtttt aagcaagata aagttaaaaa attgaccttg tttgatgact caaaagttaa
     5101 tgagttacga gtttcgaatt tgtgagagaa cgagtagcaa ggtttgaaga atatggtgcg
     5161 attaattttg ttgatggtca ggagtttgct aattgcgaga catacggtgc tggttcgaga
     5221 taagagtttg cgaggaaaca aaaaaattgt ggaatgatgg aataaaaatt atctttttat
     5281 agcaattgtt ggcattggct ggggatcagt agtttcactt ccatttgcaa ttataagtga
     5341 gaaagttgat caatcaaaaa tgggtttata tatggaaata tttaatctat cagtagtgtt
     5401 gcctcagttg gtagcgagtt tcaaaatggg tgaggttata aatatggctg ctaataaatc
     5461 cataattttt gctatttgta ccatcacact taccatttca ggacttttat ggttttttgt
     5521 gaaaaacgag aaataaaata ggagaaaaat gatttttttg ggaaaatcta ttttccccct
     5581 gcaaaaagcg atttataacg tataatattt tgag
//
LOCUS       DAOTKS010000060         5603 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_13079_length_5603_cov_4.016042, whole genome shotgun sequence.
ACCESSION   DAOTKS010000060 DAOTKS010000000
VERSION     DAOTKS010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13079_length_5603_cov_4.016042"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="P5236_02005"
     CDS             complement(<1..579)
                     /locus_tag="P5236_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63167.1"
                     /translation="MKKKKGKISLLLQKLRFKYRVSVVNENTLEESWHIHLSRFTVIL
                     YFFLFTILTFVLLTILIYVTPLRYYLPGYGETSDRSKIIQESLKTDSLMKVVSQQSAY
                     IELIKKIINEEIQPDSISSLDSVTLSKKSQELLEKSKKEEEFCKKFEEEEKFNLANIS
                     INKNENTYVFFRPVQGVITSSCSLQNHRYGINI"
     gene            complement(773..1222)
                     /locus_tag="P5236_02010"
     CDS             complement(773..1222)
                     /locus_tag="P5236_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HRU63168.1"
                     /translation="MMGPIILAYIGIGLMVGLSGVGSAVGVTIAGNATLGALKKNPDA
                     FGNYMVLSAIPGTQGLYGFLGYFLLSPHLVPDMTWGAAAAVFGGSLALALTGLLSAIR
                     QGQVCANGIASIGSGYDVFGRTLILAAFPELYAIVSLAAVFLIGNAI"
     gene            complement(1270..>2843)
                     /locus_tag="P5236_02015"
     CDS             complement(1270..>2843)
                     /locus_tag="P5236_02015"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HRU63169.1"
                     /translation="ENKLAQKESLEQKLQQIEKERDRMEVWGIFSFERLHQLREAGYI
                     INFFSCNIRKFDQEWEVIYNAFEIDTVGSTRYFITITRPGEVVDIDADPIKLSDRTAP
                     ELDLQISEIRKEIEEVKQQLIKMAVEDYNTLKSFQVETFETIDFTKVVLNTEPTIEEK
                     VMLIEGWCPEDSEEELQNFLNDSEIYYETSEPVGEEKVPVKLKNNRFARIFEFIGNLY
                     DLPNYYEIDLTPFFAPFYMLFFGICLGDAAYGLLLVLFAIFLRKKVNDAIKPVLSLMI
                     CLGISTVIMGIITGTFFGFNLIEAEIPWLEKLKKIMIDSNQLFYISLILGVIQILFGM
                     IIKAVGKVLRYGWPASFSTWGWLSILIGCGGTFVLNSYAGLPFTVARWLYIIFGGIGV
                     IFIFLLNNIHQNPLINIGSGLWDTYNRATGLLGDILSYVRLFALSISGAVMGFVFNDL
                     AINISKGIPFFGPIVMIIIMLIGHSINIFMSGLGAFVHPMRLTFVEFYKNAGFEGGGK
                     KYKPFAHYKEEKSII"
     assembly_gap    2844..2943
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2944..3260)
                     /locus_tag="P5236_02020"
     CDS             complement(<2944..3260)
                     /locus_tag="P5236_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HRU63170.1"
                     /translation="MIVKMTKYTFLVYHKQYIEFLEKIREIGVLHVIEKPEGIPENDA
                     LREKMLLSARIKIALKQLEKRLSKEIIPLPADPVKDGLEVLINIENKLAQKESLEQKL
                     QQI"
     gene            complement(3257..3862)
                     /locus_tag="P5236_02025"
     CDS             complement(3257..3862)
                     /locus_tag="P5236_02025"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HRU63171.1"
                     /translation="MAIQFQYNKTSLQNLEKNLKIRVRALPTIKNKESALRTEVKKAK
                     DDIKKINAEFEARIAAYDKMLALWGEFDTSLLEVEDIKMSIKKIAAVRIPVLDEVVYK
                     TKSFSLFNAPKWYLDGLALLKELAQIAIEHEFYIQKLNLLEHERKKTTQKVNLFEKVQ
                     IPGYEDAIRKIKRFMEDEENLSKSSQKIIKSRHEKIEGRNI"
     gene            complement(3906..5225)
                     /locus_tag="P5236_02030"
     CDS             complement(3906..5225)
                     /locus_tag="P5236_02030"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HRU63172.1"
                     /translation="MATKAFQKIYTQISQITKATCSLHATNVGNDELATVNGKLAQVV
                     KIIGDEVTLQVFEGTEGIPTNAEVVFLGKAPTLKVSEQLAGRFFNAFGEVIDGGPEIE
                     GEEREIGGPTVNPVRREQPSELIATGIAGIDLNNTLVSGQKIPFFADPDQPYNEVMAN
                     VALRAQTDKIILGGIGLTNDDYLYFKNVFNEAGALDRIISFINTTEDPPVERLLIPDM
                     ALTAAEYFAVDKNEKVLVLLTDMTLYADALAIVSNRMDQIPSKDSMPGSLYSDLAKIY
                     EKAVQFSAGGSITIIAVTTLSGGDITHAIPDNTGYITEGQLFLRRDTDIGKVIVDPFR
                     SLSRLKQLVIGIKTREDHPQVMNAAVRLYADAANAKTKLENGFDLTDYDERALAFAKE
                     YSEKILAIDVNIDTTTMLDVTWKLFAKYFKPSEVNIKQDLVDKYWRN"
     gene            complement(5279..>5603)
                     /locus_tag="P5236_02035"
     CDS             complement(5279..>5603)
                     /locus_tag="P5236_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HRU63173.1"
                     /translation="DGVPVDYHITFWKSELIDFVILQQDAFDEIDALTPIERQHYMLS
                     LIMNICNADFKFSNFVEVMDYFKRIINICKQMNYSVFHSEDFEKYEKELNKILEERKI
                     INSSN"
BASE COUNT         1850 a         1013 c          738 g         1902 t
ORIGIN      
        1 aatattaatt ccatagcggt gattctgtaa gctgcaagaa gaagttatta ccccttgcac
       61 aggtcgaaaa aaaacataag tattttcatt cttattgata ctgatatttg ctaaattgaa
      121 tttttcttct tcttcaaatt ttttacagaa ttcttcctct ttttttgatt tttcaagtaa
      181 ttcttgagat tttttactta gggtaactga atcaagcgaa gagatagaat caggttgaat
      241 ctcttcgttt attatttttt taataagctc tatataagct gattgttgac tgactacttt
      301 cattaaagaa tccgttttca atgactcttg aattattttt gatcgatcgc ttgtttctcc
      361 atagcctgga agataatatc gcaaaggtgt tacataaata agaattgtta ataaaacaaa
      421 agtcagtata gtgaaaagaa aaaaataaag aataacagta aatcgcgaca aatgaatatg
      481 ccacgattcc tccaaagtat tttcattgac aacagaaaca cgatatttaa aacgtagttt
      541 ttgtaataat aatgaaattt ttcctttttt ctttttcatt caatgattac tgaatacaaa
      601 atgctttaca aaattagcat atttcgaaag aaaataaaaa tcaagaagtt caaagtgttt
      661 tttaaattga actccttgat aaaaaaatga aaaaataatt ttgtttttta ctatagttga
      721 aaaatattac gctgtttttc tttatcgtat tttttttgat aaactctttt tattagatag
      781 cgttaccaat aagaaatacg gcagctaaag aaacaatcgc atataattct gggaaagctg
      841 ctaaaataag agttctacca aatacatcgt aacctgaacc gatactagca ataccatttg
      901 cacaaacttg tccttgacga attgctgaaa gtaatcctgt aagagctaat gctaaacttc
      961 ctccaaatac agcggcggca gcaccccatg tcatatcagg caccaaatga ggagacaata
     1021 aaaaatagcc caaaaaacca tataaacctt gtgtaccagg aatagcgctt aaaaccatat
     1081 aattaccaaa agcatcagga ttctttttta aagcacctaa tgtggcattc ccagcaattg
     1141 ttactccaac agcacttcct acccctgaaa gtcccaccat taaaccaatt ccaatataag
     1201 ctaaaataat aggtcccata atttattttt ttaattgtta atgttttata tattttgatt
     1261 ttttattctt taaataatag atttttcttc tttataatgt gcaaatggtt tatatttttt
     1321 ccctccacct tcaaaacctg catttttata aaattccaca aaagtcagac gcatgggatg
     1381 aacaaaagca cctaaaccag acatgaatat attaatactg tgtcctatga gcataattat
     1441 tatcataaca atgggtccaa aaaaaggaat acctttgctg atattaattg ccaagtcatt
     1501 gaatacaaat cccattactg ctcctgaaat acttaatgca aacaatcgaa cataagacaa
     1561 aatatctccc aacagaccgg ttgccctatt ataagtatcc cataaaccag aaccaatgtt
     1621 tataagagga ttttggtgaa tattgttcaa taaaaaaata aatattaccc caattccacc
     1681 gaaaatgata tataaccatc ttgctactgt aaaaggaaga ccagcataag aatttaaaac
     1741 aaaagtgcct ccacaaccta ttaaaataga taaccaaccc catgttgaaa aagatgctgg
     1801 ccaaccataa cgtaagactt tcccaactgc tttaataatc attccaaata aaatttgaat
     1861 aacacccaga attaaagaaa tataaaacaa ttgatttgaa tcaatcatga ttttttttaa
     1921 cttctctaac catggtattt cggcttctat taaattgaag ccaaaaaatg tgcctgttat
     1981 tattcccata attacagtag aaattcctaa acaaatcatc aaggaaagta caggttttat
     2041 cgcatcattg actttttttc gaaggaaaat tgcaaataaa actaaaagta aaccataagc
     2101 agcatctcct aaacatattc cgaaaaagag catataaaat ggagcaaaga aaggagttaa
     2161 atcaatttca taataattag gtaaatcata caaattgcca ataaactcaa atattcgagc
     2221 aaaacgatta ttttttaact ttacagggac tttttcttct ccaacaggtt cgcttgtttc
     2281 ataataaatt tcagaatcat ttaagaaatt ttgtaattcc tcttccgaat cttccggaca
     2341 ccaaccttca atcaacataa ctttctcttc aatagtaggt tcagtattaa gcacaacctt
     2401 tgtaaaatca attgtttcaa aagtttcaac ttgaaaagat tttaaagtat tataatcttc
     2461 aaccgccatt tttattagtt gttgctttac ctcctcaatt tctttcctta tttctgaaat
     2521 ttgcaaatct aactctggag cagttctatc cgacaattta ataggatcgg catctatatc
     2581 aacaacttcg ccaggtcgag taatggtaat aaaataacgg gttgaaccta cggtatcaat
     2641 ttcaaaggca ttataaatta cttcccattc ttgatcaaac ttgcgaatat tacaactaaa
     2701 aaaattgatg atataacccg cctcacgtaa ttgatgcagc ctttcaaagc taaaaattcc
     2761 ccaaacttcc attcggtctc tttctttctc aatttgctga agtttttgtt caagtgattc
     2821 tttttgagct aatttatttt ctannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnntcaattt gctgaagttt ttgttcaagt gattcttttt gagctaattt attttctatg
     3001 tttatcagca cttctaagcc atctttaaca ggatccgctg gcaatggtat tatttccttt
     3061 gaaagtcttt tttccaactg tttcaacgca atttttatgc gagctgataa cagcatcttt
     3121 tctcgcaaag catcattttc aggtattcct tcgggttttt ctatcacgtg gagcacacca
     3181 atctcgcgga ttttttctaa aaattctata tattgtttgt ggtataccaa aaaggtatat
     3241 tttgtcattt taacaatcat atattcctcc cttctatctt ttcatgacgt gatttgataa
     3301 ttttttggga agatttcgac agattttcct catcttccat aaatcgtttt atttttcgaa
     3361 tcgcatcttc ataaccgggt atttgtactt tctcaaataa atttactttt tgagtcgtct
     3421 tttttctttc atgctccaac aagttaagct tttgaatata aaactcatgc tcaatagcaa
     3481 tttgtgctaa ttcttttaat aaggctaacc catctaaata ccatttaggc gcattaaata
     3541 agctaaaaga tttggtttta taaactacct catctaaaac tgggatacgt actgcagcaa
     3601 tcttttttat actcatttta atatcttcca cttcgagcaa gctggtgtca aattcgcccc
     3661 acaaagccaa cattttatca taagcagcaa tacgagcttc gaattcagca tttatttttt
     3721 ttatatcatc ttttgctttt ttgacttctg tacgcaaagc actttcttta tttttaattg
     3781 taggtaatgc tctgactctg atcttgagat ttttttcaag attttgtaac gaagttttat
     3841 tatattgaaa ctgtattgcc attaaaaaaa ggaatttatt ttaattttta taaaaaattt
     3901 atgaattaat ttctccaata tttatccact aaatcttgtt taatattaac ctcacttggt
     3961 ttaaagtatt tagcaaacaa tttccaggtg acatccaaca tggtagtagt atcaatattt
     4021 acatcaatag ctaatatttt ttctgaatat tctttagcga aagccaatgc acgttcatcg
     4081 taatcggtca aatcaaatcc attttcaagt tttgttttag cattggcggc atcagcataa
     4141 agacgtacgg cggcattcat tacttgtgga tgatcttcac gcgttttgat acctattacc
     4201 aattgtttta gtcgtgaaag cgaacggaaa ggatccacta ttactttgcc tatatccgta
     4261 tcacggcgaa ggaataattg tccttcagta atgtatcccg tattatcagg aatagcatgg
     4321 gtaatatctc cacccgacag agtagttact gcaataatcg taatggagcc tcccgctgaa
     4381 aactgaactg ctttttcata aatttttgca agatcagaat acaaagaacc cggcatagaa
     4441 tcttttgatg gaatttgatc catacgattc gaaacaattg ctaatgcatc agcatacaaa
     4501 gtcatatctg taagtagtac caatactttt tcgtttttat ctactgcaaa atattccgca
     4561 gcagtcaaag ccatatccgg aattaataaa cgttccacag gtggatcttc tgtagtattt
     4621 ataaaactta taatccgatc taaagcccct gcttcgttaa aaacattttt aaaataaaga
     4681 tagtcatcgt tagttaatcc tataccacct aaaattattt tatcagtttg agcacgtaag
     4741 gcaacattag ccataacttc attataaggc tggtcaggat cggcaaaaaa gggaattttc
     4801 tgtcctgaaa ccaacgtgtt attcaaatca atacctgcaa ttcctgttgc aattaattca
     4861 gacggttgtt ctcgtcggac aggattgacg gttggtccac ctatttcacg ttcttcacct
     4921 tcgatttcag gtccaccgtc gataacttca ccaaaagcat taaaaaaacg tcctgctaac
     4981 tgttcactta ctttcaaagt tggggctttt cctaaaaaaa caacttcggc atttgtggga
     5041 ataccttctg tgccttcaaa gacctgtaaa gtaacttcat ccccaataat tttaactact
     5101 tgagccaact taccattgac agtagctaat tcatcattac ctacattggt ggcatgaagg
     5161 gaacatgtgg cttttgtaat ctgactaatt tgtgtataaa ttttttgaaa tgcttttgtt
     5221 gccatcttta tgattaaata ataaaaaatt ctacttaaat aaatttatta aaattgcatc
     5281 aattacttga atttattatt ttcctttcct ccaaaatctt attcaattct ttttcatatt
     5341 tttcaaaatc ttctgaatga aaaacagaat aattcatttg tttgcagata ttaataattc
     5401 ttttgaaata atccatcact tcaacaaaat tactgaattt aaaatcggcg ttacaaatat
     5461 tcattattaa acttaacata taatgttgtc gttcaatagg cgttaatgcg tcaatttcat
     5521 caaaagcatc ctgctgaaga atcacgaaat caattaattc tgatttccaa aaagtaatat
     5581 gataatccac cggcacccca tcg
//
LOCUS       DAOTKS010000061         5532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_13274_length_5532_cov_3.686690, whole genome shotgun sequence.
ACCESSION   DAOTKS010000061 DAOTKS010000000
VERSION     DAOTKS010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13274_length_5532_cov_3.686690"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..625
                     /gene="yihA"
                     /locus_tag="P5236_02040"
     CDS             26..625
                     /gene="yihA"
                     /locus_tag="P5236_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HRU63174.1"
                     /translation="MEIKSATYVKSSENYLKCPEGNLPEYAFIGRSNVGKSSLINMLT
                     HRKKLTMTSSKPGKTLLINHFLINNQFYFVDLPGYGYSSLQKEKRKKLSKMIEDYILY
                     REQLVNLFVLLDVRLDPQKIDIEFIEWLGKNKVPFSLIFTKIDKLSTSQVNEKIQNYQ
                     NILLQQWEELPPVFLTSSLKQLGRKELLQYVENINKNLK"
     gene            complement(1045..1482)
                     /locus_tag="P5236_02045"
     CDS             complement(1045..1482)
                     /locus_tag="P5236_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRU63175.1"
                     /translation="MPALSTSSLPDIIFMLLFFFMAVTSLKEVTFKVRISPPLATEVQ
                     KLENKSLIRYIYVGKPLPQYQKIYGSETRIQLSDAFADVSEIAEYIVNERSAMKEEDQ
                     NLMTVCIKADRDTHMGIINDIKQALRRAYALKIIYAANPRPTS"
     gene            complement(1502..2107)
                     /locus_tag="P5236_02050"
     CDS             complement(1502..2107)
                     /locus_tag="P5236_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRU63176.1"
                     /translation="MGKRKKRKVGQINASSMADISFLLLIFFLVTTSLNVGTGLARRL
                     PPPLPPDQKPPDIDIKKRNLFVVRINSKNQLMVQGEIIDIKDLRRKAKEFIKNENNDP
                     NLPELVTENIEYLGPMTFTKNHVISLQNDVGTTYQAYIDVQNELIAAYNELRDELAMQ
                     RFGKHYHELNEKLQGAIQKVYPQKISEAEPKNYGGVKSGTN"
     gene            complement(2145..2624)
                     /locus_tag="P5236_02055"
     CDS             complement(2145..2624)
                     /locus_tag="P5236_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63177.1"
                     /translation="MSKIVEKIPTIIAITLVSISVILVLLIYVGGSGESIYNSAGELM
                     SVPRFTDVLIYWTYFLVALTIVITLVMVGRTNVKSYIINPQSAIKSLIPIILFILVFV
                     LSWIFGSSEEVKIIGYDGNQNVGDWARFCDMMIYPVYVMLIALILTIIGAQIYKSLK"
     gene            complement(2644..3465)
                     /locus_tag="P5236_02060"
     CDS             complement(2644..3465)
                     /locus_tag="P5236_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRU63178.1"
                     /translation="MKKVFAILSILAIISFGSTVNVSAQEVASQTDQAAVNNDTASAM
                     VTDTAQAVEEVQAPAPAEGGGMHKALKQKFIEGGPAWMAPIALCLIIGLALCIERIIY
                     LSLSSTDTKKLLADIDEAWSKGGVEAAKEVCRNTRGPVASIFYQGLSRFDEGVEVVEK
                     TVASYGGVQLGLLETNLTWISLFIAISPSLGFLGTVVGMIEAFDRIQQVGDISATIVA
                     SGIKVALLTTVFGLIVAIILQVFYNYILTLVEGMTNDMEDSSISLLDIIVKHTRK"
     gene            complement(3542..3631)
                     /locus_tag="P5236_02065"
     tRNA            complement(3542..3631)
                     /locus_tag="P5236_02065"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3595..3597),aa:Ser,seq:gga)
     gene            complement(3729..4508)
                     /locus_tag="P5236_02070"
     CDS             complement(3729..4508)
                     /locus_tag="P5236_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRU63179.1"
                     /translation="MIDTHSHIYLEEFDEDRKEVIQRAKDIGVQYIILPNIDSSTLPR
                     VHQVEKEERDYCFAAIGLHPTSVKENFKEELEVIKSELQQRKYIAIGEIGIDLYWDKK
                     FYKEQEEAFCQQVEWAIKYELPILVHIRNSFEETINALSSYKGTKLSGIFHSFAGTIE
                     EAEKILNFDKFKIGINGILTFKNSKLSKIIENIGLNHIVLETDAPYLAPVPFRGKRNE
                     SAYIYYICQHLANVLKKPMDEVDQQTTRNANEIFKLFYRDS"
     gene            complement(4523..>5532)
                     /gene="guaA"
                     /locus_tag="P5236_02075"
     CDS             complement(4523..>5532)
                     /gene="guaA"
                     /locus_tag="P5236_02075"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRU63180.1"
                     /translation="HTVNGMLLFENFLDICECKRDWTPASFVESTIEQLKKQLGNDKV
                     ILGLSGGGDSSVTAMLLSKAIGKNLTCIFVDHGLLRKNEFEEVLSEYQHLGLNVIGVD
                     AKDRFYSALKGITDPEQKRKIIGAKFIEVFNEEAKKIEGVKWLAQGTIYPDVIESMSP
                     SGNTIKSHHNVGGLPEKMDLKLCEPLRLLFKDEVRKVGIELGMMHQFIKRHPFPGPGL
                     AVRILGEVIPEKVQILQEADDIFIKNLKEWNLYDKVWQAGAILLPIQSVGVMGDERTY
                     ENVVALRAVTSTDAMTADWAQLPYEFLSKVSNDIINKVKGINRVVYDISSKPPATIEW
                     E"
BASE COUNT         1783 a         1004 c          777 g         1968 t
ORIGIN      
        1 ttttttattg tttgattaaa aaaacatgga aattaaatcg gcaacatatg taaaaagtag
       61 tgaaaattat ttgaagtgtc ctgaaggaaa tttacctgaa tatgctttca ttggacgttc
      121 taatgttggt aaatcatcct taattaacat gttgacacat cggaaaaaat taaccatgac
      181 ttcctcaaaa ccaggaaaaa ctttgttaat taatcatttt ttaattaaca atcaatttta
      241 ttttgtcgat ttgccaggat atggttactc atcgttgcag aaagaaaagc gaaaaaaact
      301 ttcaaaaatg attgaagact atattcttta tcgtgaacaa ttagtaaatt tatttgtatt
      361 actcgatgtt cgactcgacc ctcaaaaaat cgacatcgaa tttattgaat ggcttggaaa
      421 aaacaaagtc cctttttcat tgatatttac aaagattgat aaattatcaa ctagtcaggt
      481 aaacgaaaag attcaaaatt atcaaaatat tttactccaa cagtgggaag aattaccacc
      541 ggtattttta acttcttcct taaaacaatt aggtaggaaa gaattgcttc aatatgtgga
      601 aaatattaat aaaaatctca aataattctt ataggtaatg atgtaaggaa tgcgagttag
      661 caagatttca tattatcggg gaataaggat ttttgtataa gtataatact ttacgctaaa
      721 caaatattta attgccaagc caattttaaa taattaaaaa tttttgtact tatattttgt
      781 ttcactcttt aacgagtaag aatttcctac tttcagagct ttaaaaaaca acctcaattt
      841 tgcaattttt cgttgataca ttatattgta attcaaataa ttataaatta tttttctaat
      901 attgccatta gaaatttcac ttaatagcaa attttagatt agccccatct ttacggttat
      961 gtgaaaagat cggaaaataa aataggagga aactatgatt tattttcctc ctactcgatt
     1021 tattttttat gttgtcaata gtgtttaaga tgttggacgt ggattggcag cataaataat
     1081 tttcaacgca taagctcttc gtaaagcttg tttaatatca ttaatgatac ccatgtgtgt
     1141 atctctatcc gctttgatac atactgtcat caaattttga tcttcttctt tcatagcact
     1201 ccgctcattt acaatatatt ccgctatttc agatacgtca gcaaaagcat cgcttagttg
     1261 aatacgtgtt tctgatccat aaatcttttg gtattgtggt agcggtttcc ctacataaat
     1321 ataacgaatc aacgacttgt tttctaactt ttggacttca gttgcaagag gaggactaat
     1381 tctgacttta aaggtcacct ctttcaatga agtaacagcc ataaagaaga ataacagcat
     1441 aaaaataata tcaggcaatg aagatgtact caaagccggc atttctcttt tttctttttc
     1501 tctaattcgt gccacttttc actcctccat aatttttggg ttcagcttca gatatctttt
     1561 gaggataaac cttctgaatt gctccttgta acttttcatt caattcgtga taatgcttgc
     1621 cgaaacgctg catagctaat tcatctcgta attcattgta agcagctatt aactcatttt
     1681 gtacatcaat ataagcttgg taagttgtgc ctacatcatt ttgaagagaa ataacatgat
     1741 tttttgtaaa agtcatggga cctaaatatt caatattctc agttacgagt tctggaagat
     1801 ttggatcatt attttcgttt ttgataaact cttttgcctt tcttcttagg tctttgatat
     1861 ctattatttc tccttgtacc attaattgat tcttactatt tatacgcacc acaaaaagat
     1921 tacgtttctt aatatctata tctggtggtt tctgatcagg tggtaaggga ggcggtaaac
     1981 gtcttgccaa acccgttccc acattcaagg aagtggtaac aaggaaaaaa atcaaaagca
     2041 aaaatgaaat gtcagccata gaactggcat ttatttgccc tacttttctt ttttttcttt
     2101 tccccatgtt tatttttaat ttaaaataaa ttttaaaaaa tgctctattt taagctctta
     2161 taaatttgtg caccaataat tgttaatatg agggcaataa gcatcacata aacaggataa
     2221 atcatcatat cgcaaaatct tgcccaatca cctacattct ggtttccatc ataaccaata
     2281 atttttactt cctcagagct gccaaaaatc caggataata caaatactaa aataaataga
     2341 atgattggaa ttaaagattt aatggctgat tggggattaa taatataact ttttacattg
     2401 gttctcccca ccataactaa ggtgataaca attgttaaag caacgaggaa gtatgtccaa
     2461 tatatcagaa catctgtaaa acgaggaacc gacattaatt cacccgcact attataaata
     2521 gattcaccgc ttcctcctac atatattaat aataccaata ttacagatat acttactaaa
     2581 gttattgcaa taatagttgg tatcttttct acaatttttg acataatttc actatttatc
     2641 aatttatttt ctagtatgtt ttacaatgat atccagtaat gagatggaag aatcttccat
     2701 atcgttggtc attccttcta ctaaggtaag aatgtaatta taaaatactt gcagaattat
     2761 tgctacaata agtccaaaca ctgttgttaa caatgccact ttaataccac ttgctacaat
     2821 agttgctgaa atatctccta cttgttggat tcggtcaaaa gcctcaatca taccaacaac
     2881 tgttcctaag aaacctaaac ttggtgaaat agcaataaat aagctgatcc atgtaagatt
     2941 ggtttccaat aaacccaatt gtacacctcc ataagaagct acggtttttt ctactacttc
     3001 aactccttca tcgaagcggg acaatccctg atagaaaata gaggctacag gtcctctggt
     3061 attacgacat acttcctttg cagcctcaac accaccttta ctccaggcct catctatgtc
     3121 agctaacaat tttttagtat cggtagaaga aagacttaga taaataatac gttcaataca
     3181 caaagctaat cctataatta agcagagggc tataggagcc atccatgcag gtccaccttc
     3241 aataaatttc tgttttaaag ctttgtgcat tcctccacct tctgctggtg ctggtgcttg
     3301 tacttcttct acagcctgag ctgtatctgt aaccattgct gaagcagtat cattgttcac
     3361 tgctgcctgg tctgtttgag aagcaacttc ttgagctgaa acatttactg tgcttccaaa
     3421 agagataatc gctaaaattg ataagattgc aaataccttt ttcattgttt ttgtttttaa
     3481 aatttctaaa tttattgttt ttatttttat taattactaa ttctttttaa atgaagaatt
     3541 gcggagagga agagattcga actcttgata cgattcaaca ccgtatatac gctttccaga
     3601 cgcacctctt cgtccactcg agcacctctc cattttgagg tgcaagttaa aaaaaaaata
     3661 tgaaatacaa cacatttatt ttttattttt attttttgct cataaaaata ttttttattt
     3721 gatgtgtttt atgagtctct ataaaaaagt ttaaaaattt catttgcatt tctggtcgtt
     3781 tgctggtcaa cttcatccat tggttttttt aatacatttg ccagatgctg acaaatataa
     3841 taaatataag cactttcatt tcttttacca cgaaaaggaa caggtgccaa ataaggagca
     3901 tctgtttcta atactatgtg attgagacca atgttttcaa ttatttttga taacttcgaa
     3961 ttcttaaagg ttaaaatgcc attaattcct attttgaact tatcaaaatt caatattttt
     4021 tctgcctctt ctattgttcc tgcaaagcta tgaaaaatac ctgatagttt ggtaccttta
     4081 taagaagaaa gtgcatttat tgtttcttca aatgaattcc ttatatgtac taaaataggt
     4141 aattcatatt taatagccca ttcaacttgc tgacaaaaag cttcttcctg ttctttataa
     4201 aattttttat cccaatacaa atcaattcct atttcaccaa tagcaatata ttttctttgc
     4261 tgcaattctg attttattac ttccaattct tccttaaaat tttcttttac actggtagga
     4321 tgtaagccaa tagctgcaaa acaataatca cgttcttctt tttctacttg atgtacacgc
     4381 ggcaaagtac tactgtcaat gttgggtaaa ataatatatt gaacaccaat atcttttgct
     4441 cgttggatta cttcttttct atcctcatca aattcttcga gataaatatg tgaatgtgta
     4501 tcaatcaatt tctaaaaatt cattattccc attctatggt agctggaggt ttggaactta
     4561 tatcgtatac tacacgatta attcctttta ccttattgat aatgtcattg gatactttag
     4621 aaagaaattc gtaaggcaat tgagcccagt cggctgtcat agcatcagta gaagttactg
     4681 cacgtaaagc taccacattt tcataagttc tttcatcgcc cattacgcca actgattgaa
     4741 tgggaagtag aatggcaccc gcttgccaca ctttatcata caaattccat tcttttaaat
     4801 ttttgataaa aatatcatct gcctcttgca atatctgaac tttttctggt atcacttcgc
     4861 ctaatattct tactgctaat ccaggaccag gaaaaggatg tcgtttgata aattgatgca
     4921 tcatacccaa ttcaattcct actttacgta cttcgtcctt aaaaagcaat cgcaaaggtt
     4981 cacaaagttt taagtccatt ttttcaggca aacctcccac attgtgatga gatttaatgg
     5041 tatttcctga gggcgacatt gattcaatta catctggata aattgtgcct tgagccagcc
     5101 atttaacacc ttcaattttt tttgcctctt cattaaaaac ttctatgaat tttgctccga
     5161 taatttttcg tttttgttct ggatcggtaa ttcctttcaa agcagaataa aacctatcct
     5221 ttgcatctac tccaataaca ttcaatccca aatgctgata ttctgacaaa acttcttcaa
     5281 attcattttt tctcaataaa ccgtgatcca caaaaatgca agtcagattt ttcccaatag
     5341 ctttgctcaa caacatagca gttacagacg aatctccccc tcctgaaagt cccaaaatta
     5401 ccttgtcatt gccgagttgc ttttttaatt gttcaattgt tgattcaaca aaggatgcag
     5461 gcgtccagtc acgtttacat tcacaaatat ccagaaaatt ttcaaataat aacattccat
     5521 tgacagtatg at
//
LOCUS       DAOTKS010000062         5530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_13278_length_5530_cov_4.545023, whole genome shotgun sequence.
ACCESSION   DAOTKS010000062 DAOTKS010000000
VERSION     DAOTKS010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13278_length_5530_cov_4.545023"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="P5236_02080"
     CDS             complement(<1..355)
                     /locus_tag="P5236_02080"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HRU63181.1"
                     /translation="MNYYYLIAGLPDLHIEDTKLFLPLLDFKKELLVQLAPSDVELIK
                     LLFAKYDNQNFLLYLANKEAELNPLGNLTSEDWQQLITLMKETEHPNDKRLLPYIQKF
                     YAVYYEEEFWSEGISK"
     gene            complement(434..1033)
                     /locus_tag="P5236_02085"
     CDS             complement(434..1033)
                     /locus_tag="P5236_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63182.1"
                     /translation="MNTRLQELTDKIYQEGVEKGNAEAKAIIEKARAEADEIIKKAHE
                     EAAQIISEAETKAANIAKNTQAELRLFAQQAVNALKSEIANLICGEIVSNSVKAATAD
                     KDFMQKVILTLVKEWAKNQAITIETKDEKALTDYFMANAKDLLNKGVKITKVNNAKAD
                     FTIVPEGAGYKITFGEEEFIAYFKDFLRPKLVDMLFEKM"
     gene            1298..1780
                     /locus_tag="P5236_02090"
     CDS             1298..1780
                     /locus_tag="P5236_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HRU63183.1"
                     /translation="MDILLIILSGIFVFLGFIGCILPILPSVPLSYIGILLLHFTSKI
                     QFSTQFLVIWGIIVLLSQLLDYFVPILGTKKFGGSKKGIWGSTLGMVIGLFFAPWGII
                     IGPFVGAITGELIDGKTNVQAIRAGLGSFIGWIINTACKLIIAGFFIYYYFEALIKVY
                     "
     gene            complement(1767..3149)
                     /gene="hisS"
                     /locus_tag="P5236_02095"
     CDS             complement(1767..3149)
                     /gene="hisS"
                     /locus_tag="P5236_02095"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HRU63184.1"
                     /translation="MQKPTIPKGMRDFMPEEMARRNYIFDTIKNVFCRYGYQQIETPA
                     MENLSTLLGKYGEEGDKLLFKVLNSGDFLADISDNELLKRDTVKLSNKICEKGLRYDL
                     TVPFARFVVQNRDKITFPFKRYQIQPVWRADRPQKGRYREFYQCDADVVGSDSLVNEL
                     ELVQIVNEVFEQLKINVTIKINNRKILAGIAEIIGASQKILDITIAVDKMEKIGLQNV
                     NEELIEKGISPEAVAKLQPILTLKGNNAEKLDQLKLILVSSEIGLKGISEIETIFRLC
                     SNVDIKFFPELDLTLARGLNYYTGAIFEIKALDVEIGSIGGGGRYDDLTGVFGMKGVS
                     GVGISFGADRIYDVLNQLQLYPQTLQSQTQILFTALGEKELIYSFSWLQRLRSFGFNV
                     EIYPEPVKIKKQLDYANNKNIPFVAIVGENEVVSEKVMLKNMLTGNQQLVTFDELKKQ
                     LSDKLNLNIP"
     gene            complement(3213..>4253)
                     /locus_tag="P5236_02100"
     CDS             complement(3213..>4253)
                     /locus_tag="P5236_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HRU63185.1"
                     /translation="GEFEQGRPIIEKLKKEHPETKILLTFYSPSGYNVRKNYSCVDVV
                     SYLPLDTRKAAQEFLKIVKPTKAIFIKYEFWPNFLLEMEAEKIPVYCISAIFRPNQIF
                     FKKYGKWYLQLLNTFSHIFVQDNLSAKLLNEHGIQKVTVCGDTRFDRVYALSLEPKKI
                     PLIESFARNSKVIVAGSTWPEDEELLIRYIKLHPQIKLILVPHEIDKSHLYDIFKLLE
                     GKYIRYTEATEINIHTFNCLVVDAIGFLSFIYQYGKVAYVGGGFGAGIHNILEAAVWN
                     IPVVFGPNYQKFREAHELIAEGGAFSISDYDSLEKKFDELLDGEEAGKKAGAYILKNV
                     GATEKIYHLIFS"
     assembly_gap    4254..4353
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4354..4582)
                     /locus_tag="P5236_02105"
     CDS             complement(<4354..4582)
                     /locus_tag="P5236_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HRU63186.1"
                     /translation="MRILYSFGIHVYGILIFIASLFYDKARELYRGQRNALKVLREKI
                     DSQSSYVWFHAASLGEFEQGRPIIEKLKKEHP"
     gene            complement(4747..5016)
                     /gene="rpsO"
                     /locus_tag="P5236_02110"
     CDS             complement(4747..5016)
                     /gene="rpsO"
                     /locus_tag="P5236_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRU63187.1"
                     /translation="MYLTAEKKKEIFEKYGKSNTDTGSPECQIALFSYRIDHLTQHLK
                     SNKKDYSTERALVKLVGKRRRLLDYLKEKDINRYRAIIQELGIRK"
BASE COUNT         1792 a          923 c          762 g         1953 t
ORIGIN      
        1 cttttgaaat gccttcggac caaaattctt cttcataata aacagcataa aacttctgaa
       61 tataaggtaa taatctttta tcgttgggat gttcagtttc tttcatcaaa gttatgagtt
      121 gctgccagtc ttcagaagtt aaatttccca gcggatttaa ttcagcttct ttatttgcca
      181 agtatagaag aaaattttga ttatcgtatt tggcaaaaag tagttttatc agttcaacat
      241 cagaaggtgc cagttggact aataattctt ttttaaaatc aagaagagga agaaataatt
      301 ttgtgtcttc tatatgtaaa tcaggaagtc ctgcaattaa ataataataa ttcatttttc
      361 tcttttcttg ttcttcaaac cctcaatgct ttttctatca attaaattca aactccttgt
      421 ttcaagcagc taattacatt ttttcaaaaa gcatatcgac cagttttggt cgtagaaaat
      481 ctttaaaata cgcaataaat tcttcttcgc cgaaggtaat tttgtaacct gccccttcag
      541 gaacaatagt aaaatcagcc ttagcgttat tcacttttgt tatttttaca cctttattca
      601 gtaaatcttt ggcatttgcc ataaaataat ctgttaaagc tttctcatct ttggtttcaa
      661 tagtaatagc ttgatttttt gcccactctt tgaccaatgt caaaatcact ttctgcataa
      721 aatctttgtc agcagtagct gcttttacag aatttgaaac aatttcacca caaattaaat
      781 tggcaatttc tgattttaag gcattcaccg cttgttgagc aaagaggcgt aattcagcct
      841 gggtgttttt ggctatgttg gctgccttcg tttctgcttc cgaaataatt tgcgctgcct
      901 cttcatgggc ttttttaatt atctcatcag cttctgctcg ggctttttca ataatggctt
      961 tcgcttcggc atttcctttt tctactccct cttgataaat tttgtcagtt aattcttgta
     1021 atctcgtatt cataagaatt tatttaaaat aacattatgc aaatttaatg ttttattttt
     1081 agtagattat ttttttataa ttatttaaaa aaattataca ccctaaaaga ttaagaaatt
     1141 tagtaacata caaattattc cttgattttc aaatataact tttttgtgta actttttatg
     1201 ataactcatt ttttattagc tttgtacgta ttaattgtca gatgtaaatc aaaatagaat
     1261 tttactttta tttttgaaaa actaataaat taatgagatg gatattttac ttattatttt
     1321 atcaggtatt ttcgtttttt taggttttat tggttgtatt ttacctattt tgcccagtgt
     1381 tccgctaagc tatataggca ttttactgtt gcattttaca tcaaaaattc aattttctac
     1441 ccaatttctt gtcatttggg gaataattgt gcttttatcc caattgctgg attattttgt
     1501 accgattttg ggaactaaaa aatttggggg tagtaaaaaa ggtatatggg gcagcacgtt
     1561 gggaatggta atagggttat tttttgcacc gtggggaatt attataggtc ctttcgttgg
     1621 ggcaattact ggtgaactta tagatggtaa aacaaatgtt caagccattc gtgcaggatt
     1681 gggttcattt ataggatgga taattaatac agcttgtaaa ctgataattg ctggattttt
     1741 tatctattat tattttgaag cacttattaa ggtatattaa gatttaattt atcagacaac
     1801 tgttttttta attcatcaaa agtaactaac tgttgattgc cagttaacat atttttcaac
     1861 attacttttt cggaaaccac ttcattttcg ccgacaatag caacaaaagg aatattttta
     1921 ttatttgcat aatccaattg ctttttgatt ttaacaggtt caggataaat ttcaacgttg
     1981 aatccaaaac tccgtaacct ttgcaaccac gaaaaactat atatcaattc cttttcacca
     2041 agtgcagtaa ataaaatttg ggtttgcgat tgtaaggttt gtggataaag ttgaagttga
     2101 ttaagcacat cataaattct atctgctcca aaagaaattc ctactccaga aactcctttc
     2161 atcccaaaaa cacctgtaag atcgtcataa cgaccaccac ctccaatgct tcctatttca
     2221 acatctaaag cttttatttc aaaaatagca cctgtgtaat aattcaaccc tcgagccaaa
     2281 gtcaaatcaa gttctggaaa aaatttgatg tccacattgc tacaaagccg aaaaatggtt
     2341 tcaatttccg aaattccttt tagaccaatt tcagaggaaa ccagtattaa tttcaattgg
     2401 tcgagttttt ctgcattatt gccttttaaa gttaaaatgg gttgcaattt agctactgcc
     2461 tcaggcgaaa tacctttttc aatcaattct tcattaacat tttgcaatcc aattttttcc
     2521 attttgtcaa cggcaatagt aatatccaaa attttttgtg aagcacctat tatttcagct
     2581 attcctgcaa gaatttttcg attattgatt ttaattgtaa cattaatttt taattgttcg
     2641 aaaacttcat tgacaatttg aacaagttct aactcattaa ccagtgaatc acttcccact
     2701 acatcggcat cacattgata aaattcacga taacgccctt tctgaggacg atcagctctc
     2761 catacaggtt gaatttgata ccgtttaaaa ggaaaagtga ttttgtcgcg gttttgtacc
     2821 acaaatcggg caaaaggtac agttaaatca taacgtaaac ctttttcaca aattttattg
     2881 gagagcttta cagtatcacg ttttaataac tcattatcag aaatatctgc aagaaaatct
     2941 cctgaattta aaaccttaaa tagcaattta tctccttctt ctccatattt acccaacagg
     3001 gtagacaagt tttccatcgc aggcgtttct atttgttgat aaccatatcg gcaaaaaaca
     3061 tttttaatgg tatcaaaaat ataattcctt cttgccattt cttcgggcat gaaatcacgc
     3121 atcccttttg gaattgtcgg tttttgcata aaatttttta aaataataag atagatgtcc
     3181 tttttaaaat ggttcctact ttttgttaaa aactacgaga atattaagtg atatattttt
     3241 tctgtagctc ctacattttt taaaatataa gcacctgctt ttttacctgc ttcttcaccg
     3301 tcgagcagtt catcaaattt tttttcaagt gaatcataat ctgaaataga aaaagctcct
     3361 ccttctgcaa ttaattcatg tgcttcgcga aatttttgat aatttggacc aaaaactacc
     3421 gggatattcc atacagcagc ttccaaaata ttgtgaatgc cagctccaaa accaccaccc
     3481 acataagcaa ctttgccgta ttggtaaatg aaagataaaa atcctatagc gtccaccaca
     3541 aggcaattaa aagtatgaat atttatttct gtagcttcag tgtatcgtat atatttcccc
     3601 tctaataatt taaaaatatc atataaatga cttttatcaa tttcatgagg caccagaata
     3661 agctttattt gcggatgaag tttgatataa cgtatcaata attcttcatc ttcgggccag
     3721 gtgctacctg ctacaattac tttactgttg cgggcaaaag attcaatcaa aggtattttt
     3781 ttaggttcca aagataaagc atataccctg tcaaaacgcg tatcaccgca cactgtaact
     3841 ttctgaatac cgtgctcgtt aagtaatttt gctgataaat tatcctggac aaaaatatga
     3901 gaaaatgtat tcaacagttg aagataccac tttccatatt ttttaaaaaa aatctgattg
     3961 gggcggaaaa tagccgagat gcaatataca ggaatttttt cagcttccat ttcaagtaaa
     4021 aaattaggcc agaattcata tttgataaaa attgccttcg taggcttgac aattttcaaa
     4081 aattcctgtg cagcttttct ggtatctaaa gggagatagg aaaccacgtc cacgcaacta
     4141 taatttttcc tcacattata acctgatgga gaataaaaag tcaataagat tttagtttct
     4201 ggatgttctt ttttcaactt ttcaatgatg ggtcgccctt gctcaaattc tccnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnctggatg ttcttttttc aacttttcaa
     4381 tgatgggtcg cccttgctca aattctccta atgaagccgc atgaaaccat acatacgaag
     4441 attgagaatc tattttttct ctcagtactt ttaaggcatt tcgttgtcca cgataaagct
     4501 ctctcgcttt gtcataaaac aacgaagcaa taaaaattaa aataccataa acatgtatac
     4561 cgaaagaata taaaattctc attctcaaaa atatttaaaa atgaaataaa aaagattgat
     4621 aaatatgtat aaatatttat tgtcgaattt tataccacct tattgtaaaa aaataaaagt
     4681 atgaaaaaca aaaggcatct caaaaatagt tttgaaacgc cttttttgaa acacttaaaa
     4741 gtaaaattac tttcttatac ccaactcctg aattattgcc cgataacggt taatatcctt
     4801 ttcttttaaa taatcgagca aacggcgacg ttttcctacc agttttacaa gagcgcgttc
     4861 tgtactataa tctttcttat ttgactttaa atgttgagtc aaatgatcaa tacggtatga
     4921 aaacaatgct atctggcatt ctggtgaacc agtatcagtg ttagactttc cgtatttttc
     4981 aaagatttct tttttctttt ctgctgttaa atacatattt aaaattaaaa attaattatg
     5041 agtctgcaaa tttacaaatt atattttaat tccctaactt ttcttgaaaa aacttccaca
     5101 aaattttatt taatagactt ttacacaaga actgacattt taaaaaactg acataaggta
     5161 gttcaaaaca acaacaaaaa cttccgtgaa aagccatgag atagattttt aaggaaaatt
     5221 ttcatctcaa atttaaaaat tgagtgcaaa aatattcaat attttcttaa taaagaccta
     5281 aaatttgcaa gtaaaaaatt gtttgagaaa tttatttttt tcaaataaac tttgaaaaac
     5341 tctcttttaa aaaattgatt cggtgaatgt gaattaagaa aatggaaata caattatcca
     5401 tttttgatta gagttaccct atttgtagat tacaaaaaaa tgcctaattt tgtaatctca
     5461 aaaataagaa gaaaccgcta aaataagaca aaatgaagag gacatttcaa ccatcgcaaa
     5521 gaaaaagaag
//
LOCUS       DAOTKS010000063         5520 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_13298_length_5520_cov_3.517292, whole genome shotgun sequence.
ACCESSION   DAOTKS010000063 DAOTKS010000000
VERSION     DAOTKS010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5520
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13298_length_5520_cov_3.517292"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="P5236_02115"
     CDS             <1..203
                     /locus_tag="P5236_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HRU63188.1"
                     /translation="SIKELSGIVKKNVGYEGEIQWDTTKPDGTPRKLLDVTRLNSLGW
                     KPKISLEEGIKQVVANYTNYIH"
     gene            252..1592
                     /locus_tag="P5236_02120"
     CDS             252..1592
                     /locus_tag="P5236_02120"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HRU63189.1"
                     /translation="MKIAIVGTGYVGLVSGTCFAEMGVDVTCVDINQEKIDQLKKGVI
                     PIYEPGLDDMVSRNIQAGRLHFTTQPTDVLNEVEAVFIAVGTPPKKDGSADLKYVFDV
                     AHTIGQNLQQYLLVVTKSTVPVGTTFNVKKIIQEELNKRGVDIDFDVASNPEFLKEGD
                     AINDFMSPDRVIVGVENERSKEILTKLYRPFLLNNFRVIFMDIPSSEMTKYAANAMLA
                     TRISFMNDVANLCEKVGADINMIRKGIGTDQRIGNKFLYAGCGYGGSCFPKDVRALIR
                     TAEENDYEMRILKAVDEVNERQKEVLYYKLLKHFDDLHGKTIALWGLSYKPNTDDIRD
                     ATSLVIIEKLLKASCKIRAYDPVAMPETQKYISEKMKDLLTSENTVYFGKDIYDTVLD
                     ADALLLITEWKEFRMPSWNVVKRAMKFPLVIDGRNIYVKEELKGLGFEYEGIGN"
     gene            1632..2462
                     /locus_tag="P5236_02125"
                     /pseudo
     CDS             1632..2462
                     /locus_tag="P5236_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(2513..4579)
                     /locus_tag="P5236_02130"
     CDS             complement(2513..4579)
                     /locus_tag="P5236_02130"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRU63190.1"
                     /translation="MNKQSNRQIKNFLFLSLIFFSSIVSAQLNTDRILTIGKSAFYFE
                     DYVLSIQYFNQVIKIKPYLAEPYMYRAMAKIQLGDYEGAEKDCNEAIERNPFIPQAYY
                     TRGFAKLKLNCFNEAAEDFSKALEFSPDNLYLIANRILAFEKAGNYEAALKDINEFLK
                     LSPNNTDMYYEKGRLQLALKDTIGAEKTFDKYIEMNPSSSLGWSARALLKLQRNDKEG
                     ALKDYNEAIKRNSTFAGDYINRAFLNIEKNNFRQALEDYDKAIALDSKNELAYFNRGL
                     LRAKLGDTNNALNDFKKVLALNPSSPEALWQKAILENTLGNYEEAIKNYQQLINNHPY
                     FLPAFLGIAEAYQKLGDEKNAFRYKQLAYNLETNKDKIKKEQITASTKIVQNTQNNGK
                     QRNIEIFNRFITQNASENETNSKYNNSLRGTIQDKFVDIVNEPNFILTYYSKPEEIRR
                     TNLFHLAVEEYNLQKKLSSPLKITNYEIPLTPELINQHFEKINSLSFQLEKNNQDADL
                     YFSRALEFSLIRDYTSAIEDLNKAISLRPNFMLAYFTRANIRYKLIESDNQLNKSANT
                     LTAGSETSSNKMKEQQNKLDFELILRDYDKVIQLTPDFTFAYYNKANILCNQKDFSTA
                     IIYYSKVIELDRDFAEAYFNRGLSYLFLGEDNKGLNDLSKAGELGIYKSYNIIHRFKQ
                     QSQEGK"
     gene            complement(4572..>5520)
                     /locus_tag="P5236_02135"
     CDS             complement(4572..>5520)
                     /locus_tag="P5236_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008654722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRU63191.1"
                     /translation="HFLRCGFMSMEARTGHVKAYVGGPNFRRFQYDMVKKGRRQIGST
                     IKPFLYTLAMEEGMWPCDKTINQPITLIAETGQPWTPRNSSKARIGEVVTLRWGLANS
                     NNWISAYLMSLFTPQSLVDLMHSFGIRGKIDPVVSLCLGPCEVSVEEMVDAYTAYTNK
                     GIRVEPLYVTRIEDNNGNVIANFTPKMHEIFGETTAYKMIDMLQAVVNEGTGSRVRTR
                     YGLTMPMGGKTGTTQNNSDGWFMGFTPSLVSGVWVGGEERSIHFDRMSEGQGATMALP
                     IWALYMKKVLSDADLKYSPNEQFEIPKWFNPNAGCEDNE"
BASE COUNT         1760 a          861 c          962 g         1937 t
ORIGIN      
        1 tttctataaa agaattatca ggaatagtta aaaaaaatgt aggctatgaa ggtgaaattc
       61 aatgggatac aacaaaacct gatggtaccc ccagaaaatt gctggatgtt actcgattga
      121 attctcttgg ctggaagcca aaaataagtt tagaagaagg aatcaagcag gtagttgcaa
      181 attacaccaa ttatattcat tagaaatgat aaaaacagtt tttagtcaac ttaataaatt
      241 tttgaattaa aatgaaaata gctattgtag gtacgggata tgttggactt gtttcgggaa
      301 cttgttttgc tgaaatgggt gtagatgtaa cctgtgttga tattaatcaa gaaaaaattg
      361 atcagttaaa aaaaggtgtt attcctattt acgaacctgg actggacgat atggtctcac
      421 gtaatataca ggcaggaaga ttgcatttca ctactcaacc tactgacgta ttgaatgaag
      481 tagaagccgt ttttattgct gttggtactc ctcctaaaaa agatggaagt gcagatttaa
      541 aatatgtttt tgatgttgct cacacgattg gacaaaactt gcagcaatat cttttggtgg
      601 ttacaaaaag taccgttcca gtaggtacaa cctttaatgt aaagaaaatt attcaagaag
      661 aattaaataa acgtggcgtt gacattgatt ttgatgtagc ttcaaatcca gaatttctga
      721 aagagggtga tgccattaat gattttatga gtccggatag agtgattgta ggtgtggaaa
      781 atgaacgatc aaaagaaatt ttgacaaaac tgtatcgtcc gtttctctta aacaattttc
      841 gagttatttt tatggatata ccttcctcag aaatgacaaa atatgcagca aatgctatgc
      901 ttgcaacccg cattagtttt atgaatgatg tagcaaactt atgtgaaaaa gtcggagccg
      961 acatcaacat gataagaaaa ggaataggaa ccgatcaacg aattggcaat aaatttcttt
     1021 atgcaggctg tggttatggc ggttcgtgct tcccaaaaga tgtaagagct ttgattcgta
     1081 ctgccgaaga aaatgactat gaaatgcgta ttttaaaggc tgtagatgaa gtaaatgaac
     1141 gtcagaaaga ggttctctat tacaaattat tgaaacattt tgatgatttg catggaaaaa
     1201 ctattgcttt atggggatta tcttataaac ccaatactga cgatatccgt gatgcaacgt
     1261 cattagttat aatagaaaaa cttttaaaag caagctgcaa aattcgggct tatgatcctg
     1321 tagctatgcc agaaacccaa aaatatattt ctgaaaaaat gaaagattta ctaacatcag
     1381 aaaatacagt ttattttgga aaagatattt atgatacagt attggatgct gatgcattgt
     1441 tgttaatcac cgaatggaaa gaatttcgaa tgccaagttg gaatgtagta aaaagagcaa
     1501 tgaaatttcc attggttatt gatggacgaa atatttatgt aaaagaagaa ttgaaaggac
     1561 tgggctttga atatgaagga attggaaatt gagttaaagt ttttattgaa ggtaaaagag
     1621 ttgttttttg aatggaaaca gtaattaagc ccaaaaatag tctgttagaa attaatctga
     1681 atgaaatctg gcagtatcga gatttgcttt ttatgtttgt aaagtgaaat attatcacgc
     1741 aatacaaaca aactattctt ggtcctacat ggttttttat tcaaccagct ctcactaccc
     1801 ttatgtacat gattgttttt ggtggaattg ctaaaattcc aactgatgga cttcctcaac
     1861 ctatgtttta tttggctggc gtagtttgtt ggcaatattt tgcagattgc ctgaccaaaa
     1921 catccaatac ttttattgat aatcagggaa tttttgggaa agtttatttt cctcgtttga
     1981 ttgtaccatt ggctactgtt atttcaaatc tagtaaggat gttaatccag tttctgcttt
     2041 ttattgcagt ttatatttat tatattgttg ttggagtaaa agtagccccc aatctctata
     2101 ttttattggt tcccttgtta attattatgt tggctggtct ttcactgggt tttggaatta
     2161 ttatttcttc gttaacaacc aagtatcgtg atttgaccat tttgttcaat tttattgttc
     2221 aactctggat gtatgctacc ccaattattt atccattaag tactatgcca cccaatcgac
     2281 aatggattat ggctttaaat ccagttacat ctattattga aacattcaag tacggaacta
     2341 tgggagttgg aactttcagt tgggcacact tgagttatag tttcgttttt atggcggtat
     2401 tgctgtttgt gggtataatg gtgttcaaca aagttcaacg aagttttatg gataccatat
     2461 aaaaaacttt tgcatctatc ctatcctatt aattttgtat aaaattaaaa aactatttcc
     2521 cttcctggga ttgttgttta aatcggtgaa tgatattgta acttttatag atacctaatt
     2581 caccagcttt gcttaagtcg ttcagtcctt tgttatcttc ccctaaaaat aaatagctca
     2641 atcctcgatt aaaataagct tctgcaaaat cgcgatctaa ttcaatgact ttactgtaat
     2701 aaataattgc agtagagaaa tctttttgat tacataaaat attagcctta ttataataag
     2761 cgaaagtaaa atcaggtgtt aattgaatta ctttatcata atcacgcaaa ataagttcaa
     2821 aatccagttt gttctgttgt tctttcattt tatttgatga agtttctgaa ccagcagtca
     2881 aagtatttgc tgatttattt aattgattat cagattctat taatttgtac cgaatattgg
     2941 cacgggtaaa ataagcaagc atgaaattgg gtcgtaacga aatggcttta tttagatctt
     3001 caatggcgct tgtgtaatca cgtatcagcg aaaattcaag agctcgacta aaataaaggt
     3061 cagcatcctg gttgtttttt tccagttgaa aagaaagaga attaattttt tcaaaatgtt
     3121 gattgattaa ttcaggcgtc aaaggtattt cgtaattagt aatttttaat ggtgaactta
     3181 atttcttttg tagattatat tcttcaactg ccagatgaaa aagattggta cgacgaattt
     3241 cttcaggttt gctataataa gtcaatataa aatttggttc atttacaata tccacaaatt
     3301 tatcctgtat agttccacga agtgaattgt tgtattttga gtttgtttca ttttcagaag
     3361 cattttgggt aataaaacga ttgaagattt caatatttcg ttgtttccca ttattttgag
     3421 tgttttgtac tattttagtg gaagctgtga tttgttcttt ttttattttg tctttattgg
     3481 tttcaagatt gtaagccaat tgtttgtatc taaaagcatt tttttcatct ccgagttttt
     3541 gataagcttc agcaatacct aaaaaagcag gtagaaaata aggatggtta ttaataagtt
     3601 gttgataatt ttttattgct tcttcataat tgccgagtgt attttcaaga atggcttttt
     3661 gccataaagc ttcaggactt gaaggattta atgctaaaac ttttttgaaa tcatttaaag
     3721 cattattggt atcccctaat tttgcccgta acaaaccacg attgaaataa gctaattcat
     3781 ttttactatc caaagctatg gctttatcat aatcctccaa agcttggcga aaattattct
     3841 tttcaatatt taaaaaagct ctattgatat aatctccagc aaaagttgaa tttcttttta
     3901 tggcttcatt gtaatctttt aaagctcctt ctttgtcatt tctttgtaat tttagtaatg
     3961 ctcttgcact ccatcctaag ctactggaag gattcatttc aatgtattta tcaaaagttt
     4021 tttctgcacc aattgtgtct ttcaaagcaa gttgcaatct acctttttcg taatacatgt
     4081 cggtattatt agggctaagt ttaagaaatt catttatatc ttttaaagcg gcttcataat
     4141 ttccagcttt ttcaaaggct aatattctat ttgcaatcaa atacaaattg tcgggactga
     4201 attcaagtgc ttttgaaaaa tcctcagcag cttcattaaa acaatttaat tttaatttgg
     4261 caaaaccacg agtataatag gcttgaggta taaatggatt tcgttcaata gcttcgttac
     4321 aatctttttc agcaccctca taatctccca gttgaatttt tgccattgcc ctgtacatgt
     4381 aaggctctgc taaataaggc ttgattttaa taacttgatt aaaatattga atcgaaagaa
     4441 catagtcttc aaaataaaat gcacttttgc caatggtaag aatacgatcg gtattcaatt
     4501 gtgctgaaac aattgaagaa aagaatatca aggacaagaa aagaaaattt tttatctgtc
     4561 tgtttgattg tttattcatt atcttcgcat cctgcattgg gattaaacca ttttggaatt
     4621 tcaaattgtt cgtttggtga atattttaaa tcagcatcag agagaacttt tttcatatat
     4681 aaagcccaaa taggtagagc catagtagca ccttgaccct ccgacatcct atcgaaatga
     4741 atagagcgtt cttcaccgcc tacccaaacg cccgaaacca atgatggcgt aaagcccatg
     4801 aaccatccgt cggaattgtt ttgcgttgtt cctgtttttc cgcccattgg catagtgaga
     4861 ccgtaacgag tgcgcaccct tgaacctgtt ccttcattta ctacggcttg tagcatatca
     4921 atcattttat aagctgttgt ttctccgaaa atttcatgca ttttaggagt aaaattggca
     4981 attacatttc cattattgtc ttcgatacgt gttacataca atggttcaac acgaataccc
     5041 ttattggtat aggcagtata agcgtcaacc atttcttcta ctgaaacttc acacggacct
     5101 aaacacaaag aaacaacagg atcaattttt cctctaatac caaatgaatg catcaaatct
     5161 acgagagatt gtggtgtaaa taaactcatt aaataagctg atatccagtt atttgaatta
     5221 gccaagcccc aacgtagtgt tactacttct cctattctgg cttttgaaga gtttcgagga
     5281 gtccagggtt gtcctgtctc agcaattaag gtaatgggct gattaatagt tttatcacaa
     5341 ggccacatac cttcttccat tgctaaggta tataagaagg gttttatggt agaacctatt
     5401 tgtctacgtc ctttttttac catatcatat tgaaaacgtc taaaatttgg tccgcctaca
     5461 taagctttta catggccagt acgagcttcc atagacataa atccacatct taaaaaatgt
//
LOCUS       DAOTKS010000064         5518 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_13307_length_5518_cov_4.206846, whole genome shotgun sequence.
ACCESSION   DAOTKS010000064 DAOTKS010000000
VERSION     DAOTKS010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5518
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13307_length_5518_cov_4.206846"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..1702)
                     /locus_tag="P5236_02140"
     CDS             complement(170..1702)
                     /locus_tag="P5236_02140"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HRU63192.1"
                     /translation="MSDKKNSPFSLEEPLEYLSERAIERRKFFSIPLDSTDLPINPSY
                     LNLIQQTGAKIHCCSKWLNGLTVLLQDSNLMANIRSLPFVKWTQYTGKTTSPSAKISK
                     KTKFKVPAEQNDYANQIKQVGGDYLLKNGLRGKNIHIGVLDAGFTHVNINPAFDSLRL
                     QNRLLGTKDIVSLSDLDIFEAHFHGANVLSIMTANLPEQYIGSAPGASYWLIRTENDS
                     SEYLVEIDFWVAGIEYADSVGVDLVNSSLSYTIFDESSMDFQYEDMNGENSRASRAAT
                     IAAQKGIIVVVSAGNEGNKTWKYIGSPADAKETITVGGVRADGNPSAFSSFGPTFDGR
                     VKPEICARGTSVEMIDVNGNLTYGNGTSYAAPIITGLMAGYLQYFKENQIHITIHEIL
                     QSIFRTGNLYFNPTDQMGYGIPNFQAALENYLTKNEMIIANNVQWFINKSDETIHIQL
                     PKEIVGEKVQVRVYSDMGILRHQQEISSEEIIISTSSFLPGIYLIHIRYDNKLVMKKI
                     II"
     gene            complement(1936..4764)
                     /locus_tag="P5236_02145"
     CDS             complement(1936..4764)
                     /locus_tag="P5236_02145"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRU63193.1"
                     /translation="MINKDFRVKRFILLTALTLFSLSLFSQQQFQPLPIDPKVRYGKL
                     ENGLTYYIRSNQFPKQRADFYIVQKVGAILEEDYQNGLAHFLEHMAFNGTVHFPGKKI
                     IDYFESIGVKFGQNINAYTSLDETVYHLSNVPTIRESIIDSALLVLHDWSHFILLEDD
                     EIEAERGVILEEWRQGQNAYRRMWAESNKKKFPGSQYAKRDIIGDTAVIKNFKPDELR
                     EYYNKWYRPDLQAIIIVGDVDVDKVEAKIKELFTDIKKNENAPERPIYPIFDNEEPII
                     SIVTDPEAPQTQIELYYKHNKLPAEIKLSMNGYVIELINSLISNIMQDRFDEITMKPD
                     APFVAGYANYGELVKSKDAFQLLVIPKEGQELEGLEALLLEAEKIKRFGFVNSELERA
                     KANLIKQVEKRYNERENLKNSEFVKEYIGHFLNNEPIPGIEWEYQTLQMILPQVNVDM
                     VNQLAKSYVTDTNMIVSIMAPEKETVKIPTEEQIRQLIENTKTVELTPKVDEQINKPL
                     IGQVPKAGKIKKVTKNSTLETTEWLLSNGVKVILKPTKFKKDEILLNAYSIGGLAKII
                     NNEDLISGAFASEIVSNNGLAEFSQIDLKKLLTGKIVSVRPYISSYEEGFSGNSSVSD
                     FETMLQLIYLHFTALRRDDDAFTALMNTYKAILSNSEKNPDKAFSDSITVMLTNHHPR
                     TILLNLSTIEKVNQDKALSIFAERFANPADFTFIFTGNIDPQNESLKKFICTYLGGLR
                     TTNMKETFTDDQIRKPEGRVNNHFTKEMKVKKASNFIAYHGQMPFNLQNRTAMSAIGN
                     ILNIRYLESVREKEGGSYGVGVRGSIDNKPIEDAILMIQFDTDPEKQPKMISIIYDEI
                     NNIIANGPRKDDVQKVKETMLKKYAEDVENNGWWQNAIYLYYQDHLNLVTDYKSSVEE
                     VTPLFIQTTLKKLVDQNNVIEVIMMPKE"
     gene            4979..5482
                     /locus_tag="P5236_02150"
     CDS             4979..5482
                     /locus_tag="P5236_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRU63194.1"
                     /translation="MSENLFIPINETKEEIYNNLLPQVAALIEGENDFIANLANVSAA
                     LHETFHWLWVGFYLIKNDELVLGPFQGSIACTRIGYGKGACGTAWKEKKSLLILNVKN
                     FPGHIACNQLSVAEIVVPIFDANQNIIGVLDIDSDKHDELTDVDVFYLGKICQLITNK
                     LSNFFHV"
BASE COUNT         1786 a          923 c          807 g         2002 t
ORIGIN      
        1 gtagcacacg atataatcag aagatagtga aaagtgatca aatatttgga tttggagttt
       61 ttcctcaacc cacgcttcct taaaacttac gcagatttgt aatctgggca tgccaaaggc
      121 aaaaaatact gatagaaatt gcaaatttct gccagcaatg gatttattat caaattatta
      181 tttttttcat aacaagctta ttatcatacc taatatgaat cagataaatt ccaggcaaaa
      241 atgagctggt agaaataata atttcttccg atgaaatttc ctgctgatgc cttaaaatac
      301 ccatatcaga ataaaccctt acttgaactt tttcccccac aatttctttt ggtaactgaa
      361 tatgaatagt ttcatcgctt ttgttaataa accattgcac gttgttagca ataatcattt
      421 cattttttgt caaataattt tccaaagcag cttgaaaatt aggaattcca taccccattt
      481 gatcagtggg attaaaatat aaatttccag tacgaaaaat agattgaagt atttcatgga
      541 tggttatatg aatttgattc tccttaaagt attgaagata accagccatt aatcctgtaa
      601 taataggtgc ggcataagaa gtgccattgc cgtaagttaa atttccattt acatcaatca
      661 tttcaacaga agtgcctcta gcgcatattt caggtttcac acgcccgtca aaagtaggtc
      721 caaatgatga aaaagcactt ggatttccat ctgctctaac tcctccaacg gttatagttt
      781 cttttgcatc agcaggagaa ccaatgtatt tccatgtttt atttccttca ttacctgcac
      841 taaccactac aatgatacct ttttgagcag ctatcgtggc agctctgctt gctcttgaat
      901 tttctccgtt catatcttca tactgaaaat ccattgaact ctcatcaaaa atagtataag
      961 ataaagaaga atttactaaa tctactccta cgctatcagc atattcaatt cctgctaccc
     1021 aaaagtctat ttctacaagg tattcagagc tatcattttc tgtcctgatt agccaatatg
     1081 aagcaccagg agctgagcct atgtattgtt ctggtaagtt agcagtcatg atggataata
     1141 catttgctcc gtgaaaatga gcttcaaaaa tatccaaatc cgacaatgat acaatatcct
     1201 tcgttcctaa caatcgattt tgtaatcgta agctatcaaa agctggatta atatttacat
     1261 gtgtaaatcc tgcatctaaa acgccaatat gaatattctt tcctctcaat ccattcttta
     1321 ataaataatc tccacccact tgtttgattt gatttgcata atcattttgc tcagccggaa
     1381 ctttaaattt tgttttttta gaaattttag cggaaggaga agttgttttg ccagtatatt
     1441 gtgtccattt tacaaatggt aacgaccgaa tgtttgccat aagatttgaa tcttgcaaca
     1501 aaacagttaa accgtttagc catttactgc aacaatgaat ttttgctcct gtttgttgaa
     1561 ttaaatttaa ataagatgga ttaattggta aatcagttga atctaaagga atagaaaaaa
     1621 actttcttcg ttctatggct cgctctgaaa gatattccaa tggttcttct aatgaaaacg
     1681 gactattttt tttatctgac aattgaacat aaaaaaaata attggtttct tgagaatgac
     1741 cttggcaaaa cattatccaa aaaccaaaaa ataaaaacca ctttttataa atgataattt
     1801 ttctcatcat ccctgaatat taccatttta aaaatatttt tgtattcaat tccaataaat
     1861 tagctagggt taataatgtt tattttgaac aaagaggaag atttttggct tcctctttga
     1921 aaatttggta ttttattatt ctttaggcat cattattact tctatcacat tgttttgatc
     1981 gactagtttt ttcagcgtag tttgaatgaa taatggtgta acttcttcta ccgaagactt
     2041 ataatcggtt actaaattca aatgatcttg atagtaaaga tagatagcat tttgccacca
     2101 gccattattt tctacatctt cagcgtattt tttcaacatt gtttctttta ctttttgcac
     2161 atcatctttg cggggtccat tggcgatgat attattgatt tcatcgtaaa tgatgctgat
     2221 cattttaggt tgtttttctg gatcggtatc aaattggatc ataagtattg cgtcttcaat
     2281 cggtttatta tcaatacttc cacgcactcc aacaccataa gaaccacctt ctttttcacg
     2341 aacactttcc agataacgaa tatttaaaat atttcctatg gcactcatag ccgtcctgtt
     2401 ttgtaaatta aaaggcattt gtccatgata agcaataaaa tttgaagctt tttttacttt
     2461 catttctttt gtgaaatgat tgtttactct cccttcaggt tttcttatct gatcatctgt
     2521 aaatgtttct ttcatattgg tggtacgtaa acctcccaaa taagtacaaa taaatttttt
     2581 caatgattca ttttgtggat caatatttcc tgtaaagatg aaagtaaaat cagcaggatt
     2641 ggcaaatcgt tcagcaaaaa tggacaatgc tttatcttga tttacttttt ctattgtgct
     2701 caaattcaac aaaatagtac gtggatgatg attcgtgagc ataacagtaa tactatcaga
     2761 aaaggcttta tcgggatttt tttcactatt agacaaaata gctttataag tattcattaa
     2821 agctgtaaaa gcatcatcat ctctccgtaa agctgtaaaa tgcaaataaa ttaactgtaa
     2881 catggtttca aaatcagaaa cggaagaatt ccctgagaaa ccttcctcat aagaagatat
     2941 ataaggccta acactgacaa ttttccctgt tagtaatttt tttaaatcta tttgtgaaaa
     3001 ttctgctaaa ccattatttg aaacaatttc agaggcaaac gcgccagaaa ttaaatcttc
     3061 attatttata attttcgcca aacctccgat gctatacgca ttcagcaaaa tttcatcctt
     3121 tttgaattta gtcggtttca aaatcacttt cacaccattg cttaataacc attcggttgt
     3181 ttccagtgtt gaatttttag ttactttttt tatttttcca gctttaggca cttgtccaat
     3241 taatggttta ttaatttgct catcaacttt tggtgttaat tcaacagttt tggtattttc
     3301 gatcaattgt cggatttgtt cctcagtagg gattttaaca gtttcttttt caggagccat
     3361 aatagaaaca atcatatttg tatctgtaac atatgatttg gctaattgat ttaccatgtc
     3421 tacatttact tgtggtagta tcatctgcaa tgtttgatat tcccattcga taccgggaat
     3481 aggttcgtta tttaaaaaat gacctatata ttccttgaca aactcgctat tttttagatt
     3541 ttctctttca ttatatcgtt tttcaacttg ctttattaaa tttgcttttg ccctttctaa
     3601 ctctgaattt acgaaaccaa aacgcttgat tttttcggct tcgaggagca aagcctctaa
     3661 tccttctaat tcttgccctt cttttggaat aactaataac tggaaagcat ctttcgattt
     3721 aaccaattct ccataattag cataacctgc cacgaaagga gcatcgggtt tcattgtaat
     3781 ttcatcaaat cgatcttgca taatattgga gatcaacgaa ttaataagct caatcacata
     3841 accattcatt gacaatttaa tctctgctgg tagtttatta tgcttgtaat acaattctat
     3901 ttgagtttga ggagcttctg gatctgttac aatcgagata attggttctt cattgtcaaa
     3961 aattggataa attggacgtt ctggagcatt ttcatttttc tttatgtctg taaataattc
     4021 tttaattttt gcttcaactt tatctacatc aacgtcacct acaattatta ttgcctgtaa
     4081 atctggtcta taccatttat tgtaatattc acgaagctca tctggtttaa aatttttaat
     4141 cactgctgta tctccaatta tatcccgttt agcatattgt gaaccaggaa attttttctt
     4201 atttgattca gcccacatgc ggcgataagc attttgtcct tgtcgccatt cttctaaaat
     4261 tacacctctt tctgcttcaa tttcatcatc ttctaataat ataaaatgtg accaatcatg
     4321 taaaaccaac aaagcactgt caataatact ttcacgaata gtaggtacat tcgaaagatg
     4381 atataccgtt tcatcaagtg aagtataggc attgatgttt tgaccaaatt tgacaccaat
     4441 tgattcaaaa taatcaataa tttttttacc agggaaatga acagttccat taaatgccat
     4501 atgttcaaga aaatgtgcaa gaccattttg ataatcttct tctaaaatag cgcccacttt
     4561 ttgaacgata taaaaatcag cacgttgttt gggaaattga ttggatcgaa tgtaatacgt
     4621 gagtccattt tctaatttgc cataccttac tttcggatca ataggaagag gctgaaattg
     4681 ttgttgtgaa aataacgaaa gagaaaaaag cgtcagtgca gttaataaaa taaatctttt
     4741 aactctaaaa tctttattta tcataataaa aaatttttaa aaatgaactt taaaaaacaa
     4801 ttttaattca caaagtaaca gataaatttt agaaagtaca ataaatcaat ttgctttttc
     4861 ttaaaaatgt taaatcttgt aattactatg ttctcaaatg aaactaagaa ttgaaataac
     4921 tctcagaaaa gattttttaa tgtaattttg tagtagaaat gtaaataaat aattttttat
     4981 gagtgaaaat ttatttattc cgataaatga aacaaaagag gagatttata ataatctatt
     5041 acctcaagtg gctgctttga tagaaggaga aaatgatttt attgcaaatt tagcaaatgt
     5101 ttctgctgct ttgcatgaaa cttttcattg gctttgggtg ggtttttatc tgataaaaaa
     5161 tgatgaattg gtacttggac cttttcaagg ttctattgcc tgtacacgaa ttggttacgg
     5221 gaaaggtgca tgtggaacag cttggaaaga aaagaaaagt ttattgatac tgaatgttaa
     5281 aaattttcct ggacatattg cctgtaatca actttctgtt gcggaaattg ttgttccaat
     5341 atttgatgca aatcaaaata ttattggcgt tttagatatt gacagtgata aacacgatga
     5401 attgacagat gtagatgtat tttatttggg aaaaatttgt caattgatta ccaataaatt
     5461 atctaatttt tttcatgttt aaaaaattat caacaagaaa cggatttttc actaaaaa
//
LOCUS       DAOTKS010000065         5501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_13348_length_5501_cov_5.314726, whole genome shotgun sequence.
ACCESSION   DAOTKS010000065 DAOTKS010000000
VERSION     DAOTKS010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5501
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13348_length_5501_cov_5.314726"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..1176)
                     /locus_tag="P5236_02155"
     CDS             complement(7..1176)
                     /locus_tag="P5236_02155"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRU63195.1"
                     /translation="MKLNRENRQENLKNKLNSNAGRDGKFYRKSDREDMPAEEGNKDL
                     FLTENNRIQVGKKLILKKKENSFSKTSNFLNRNNKSKDYSSDRKNENDLILKKKKNVT
                     SFPKYEKVTYNKTSSHPLKGENDRKDFHKSPYSQKKQLEYNKKNIDYSKPIRLNKFLS
                     NAGVCSRREADEFIKAGVISVNGQVVTEMGTKVMRTDKIMFRNQEVHLERKVYILLNK
                     PKDCVTTSDDPHARLTVMDLVKNACSERIYPVGRLDRNTTGVLLLTNDGDLAAKLTHP
                     KYDKKKIYQLTLDKSLKPEDYENIKKGVALENEELIEIDALEFIKEGDRKQLGIEIHS
                     GQNHVVRRIFEKYGYKVVRLDRVYFAGLTKKNLSRGKYRFLTQKEINMLKMGAYE"
     gene            complement(1397..1837)
                     /locus_tag="P5236_02160"
     CDS             complement(1397..1837)
                     /locus_tag="P5236_02160"
                     /inference="COORDINATES: protein motif:HMM:NF023109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HRU63196.1"
                     /translation="MEEEKRKIDLGKKNNEIIYSKSIKAGNRIYYLDVKKSRRDELYL
                     AITESKKKKVGFDENAQVTYEKHKIFLYREDFDKFREGLEDVMNFIKNQGEEETKTES
                     NLEEDQKSNEISDDPGEDDSVSDELDKEGNNPKPEKNFFDKFKF"
     gene            2089..3039
                     /gene="nusB"
                     /locus_tag="P5236_02165"
     CDS             2089..3039
                     /gene="nusB"
                     /locus_tag="P5236_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRU63197.1"
                     /translation="MINRVLIRIKIIQILYSYYKNEGKSVQLAEKELLHSIEKTYDLY
                     YHLLQLAIEITRYAALRIDARKNKLRPTAEDLHPNMKFVENKFVQQLSNNIQFNSYLN
                     EHKLSWTNYPEIIKNLFEEITTADFYKEYMESSLDDYEEDKNLWKKIYKKIILENEEL
                     DNSLEDQNIYWTADIELVVSFIIKTIKKFKLENSAYQELLPMFKDEEDEEFARKLLRS
                     SINNCNEFRNLINKNTKNWELDRIAFMDIVIMQAALAELMEFPTIPVNVTLNEYIEIS
                     KMYSTEKSGKFINGILDNIVGELKKENKLIKVMTFKNNKK"
     gene            3125..3457
                     /gene="yajC"
                     /locus_tag="P5236_02170"
     CDS             3125..3457
                     /gene="yajC"
                     /locus_tag="P5236_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRU63198.1"
                     /translation="MNLLTILLQAKPADQPSGGGYTGILMLILIFVVFYFFLIRPQTK
                     RQKEIKKQREAMKPGDHVITSGGIYGKIKEVKDNAFIIEIADNVRIKVDKNSVFLTAE
                     DIQNTPSK"
     gene            3518..4459
                     /locus_tag="P5236_02175"
     CDS             3518..4459
                     /locus_tag="P5236_02175"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HRU63199.1"
                     /translation="MKMFFLSKDVFIFLFFLVLSTGFWFINALSKPREKTIEVPLQYE
                     GLPPDIVITNSPPKFISLKIRDEGRNLLSYTRKKLNPLIIQLARNFQENGEIIVSTDQ
                     LLNKFTYYLQPTTLILEAKPETILIRYEKLYSKVLPVECKINFDLLPQYMISDEIKLQ
                     PSKVNVFGPKHLLDSLKTIKTELIYLKSLHDTTIITAKLQSIANVRFLPEIVEVIIPV
                     EMFTEKKVQLPITVINAPGDIYLRTLPSKVIATFNIAMSRYHSFKPSDIQVAVDYNEV
                     KNNNSDKLNVKILRYPIYVQNIRLDPQQVDFFVEKNK"
     gene            4497..4967
                     /locus_tag="P5236_02180"
     CDS             4497..4967
                     /locus_tag="P5236_02180"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HRU63200.1"
                     /translation="MLQRIQTLYLTIVLILSCLTLFSPVANLFNKEDLTFYEINYRGI
                     CLIEDDSKTPVSSVWALTVISAIVPLVTLATIFFYKKRMLQIRLSIFNLIIMIGYYVI
                     LFIYIWFATKNLPASHWNLKIVSAFPLINIILTILAMRAIVKDERLIKSLNRLR"
     gene            complement(5057..>5501)
                     /locus_tag="P5236_02185"
     CDS             complement(5057..>5501)
                     /locus_tag="P5236_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF554 family protein"
                     /protein_id="HRU63201.1"
                     /translation="EQFSEYLKRKLHLGGERFSEGLVTAFLLFCIGSMTILGAIQEGI
                     DGSSDLLFTKSLMDGFSSIILGSALGAGVIVAAIPLLIFQGGITLLAMWASSFFTSEI
                     IQGLTSVGGILLIGLGIDILGIKKLRIINLLPALIFVVLFLWWFV"
BASE COUNT         1908 a          889 c          750 g         1954 t
ORIGIN      
        1 taaaaatcat tcataagctc ccattttgag catattgatt tctttttgtg taaggaaacg
       61 gtattttcct cgtgaaagat ttttcttagt caaacctgcg aaatagactc tatctaaacg
      121 tactacttta tagccatatt tctcaaaaat acgacgaacc acatggtttt gacctgaatg
      181 aatttcaata cccaattgtt ttctatcacc ttcttttata aattcgagtg catcaatttc
      241 aatcaactct tcattttcaa gtgcaacgcc ttttttgata ttttcgtaat cttctggttt
      301 taatgattta tcaagagtta attgataaat ttttttcttg tcatatttgg gatgggttaa
      361 ttttgcagcc agatcaccat cgtttgtcaa caacaggaca cccgtggtat ttctatccaa
      421 cctgccaacc ggatatattc gttcactgca agcattttta accaaatcca tcaccgttaa
      481 acgagcatgt ggatcgtcag aggttgttac acaatctttt ggtttattaa gcaagatata
      541 gactttccgt tccagatgaa cttcttgatt acgaaacatt atcttgtcag ttctcatcac
      601 tttggtcccc atttcagtaa ctacctgacc attaaccgaa ataacacctg ctttgataaa
      661 ttcatcagcc tctctgcgtg aacatacgcc tgcattggac aaaaacttgt ttaatcgaat
      721 aggctttgaa taatcaatat ttttcttatt atattcaagt tgtttttttt gactataggg
      781 cgatttatga aaatctttcc tatcattttc accttttaaa ggatgagaac tggttttatt
      841 ataagttact ttttcatatt tcggaaaaga ggtaacattc ttcttctttt ttagtataag
      901 gtcattttca ttttttctat ccgaactata atctttgctt ttattatttc tgtttaaaaa
      961 attagaagtt ttagaaaatg aattttcttt tttctttaaa atcaatttct ttcccacctg
     1021 aatgcgatta ttttctgtta ggaacaagtc tttatttccc tcttctgctg gcatatcttc
     1081 tctgtcactt tttctataaa atttaccgtc acggccggca tttgaattta atttattttt
     1141 taaattttct tgcctatttt ccctgtttaa tttcattttg atttgataaa aaatttacat
     1201 tgcaaatttc ataaaaaaac ttggataaat cattatccta taagtcaaaa aactctaata
     1261 aattaacaat ttattttaaa aagataattc tgtcaaaatt atctcaaaag gaaagtataa
     1321 aaatacactg ctatcccttt tttgaacagc agtgtattaa gagaataaaa acagacattg
     1381 gtcgagaagt gagaatttaa aatttgaatt tatcaaaaaa atttttctcg ggttttggat
     1441 tattaccttc tttatccaac tcatcactca cagaatcatc ttcacctgga tcatcgctta
     1501 tttcattaga tttttgatct tcttcaaggt tactttctgt ctttgtttct tcttcacctt
     1561 gatttttgat gaaattcatt acatcctcta atccttcacg aaacttatca aaatcttcac
     1621 gataaagaaa tattttgtgc ttttcgtaag taacctgagc attttcgtca aaaccaactt
     1681 tctttttctt gctttcagtg atggctaaat acaattcatc tcgtctgctt ttttttacat
     1741 ctaaataata aatacgattg cctgctttga tagattttga ataaataatt tcattattct
     1801 ttttccccaa atcaattttt cttttttctt cttccatgtt ttccatcttt ttcagttaga
     1861 ttttaaataa aatagagaaa aatcccttca aaaatttaaa gttcaaattt ctatattttt
     1921 ttttacttta tcaaattatt tatctatttt ttgattttaa attatgttac ttcatttttg
     1981 ggaatagatg tgaaagtaat tttaacagac agtagtatga aaaagaagaa aaaataccta
     2041 actttgcctt ctaatttata aagtgaccat tgaagattaa ctgttgatat gattaatcgc
     2101 gttttaattc ggattaaaat tattcaaatt ttgtattctt attataagaa tgaaggtaaa
     2161 tccgtacaat tagctgaaaa agagcttctt cacagcatag aaaaaactta cgatctttac
     2221 tatcatttat tgcaacttgc tattgaaatt actcgttatg ctgctttgcg tatcgatgcg
     2281 agaaaaaaca aactgcgtcc cacagcagaa gatttgcacc ctaacatgaa atttgttgaa
     2341 aataaattcg ttcagcagtt atcaaacaat atccaattta atagttattt aaacgaacat
     2401 aaattatcgt ggacaaacta tcctgaaatc attaaaaatt tatttgaaga aatcacaaca
     2461 gcagattttt ataaggaata tatggaaagt tcgctggatg attatgaaga agataaaaat
     2521 ctatggaaga aaatttataa aaaaattatc cttgagaatg aagagctgga caactcactg
     2581 gaagatcaaa atatttattg gacggctgat attgaattgg tagtgagttt cattattaaa
     2641 acgataaaaa aattcaaatt agaaaatagt gcttaccaag aacttttgcc aatgtttaaa
     2701 gatgaagaag atgaagaatt tgctcgaaaa cttttaagaa gttctattaa taactgcaat
     2761 gaatttagaa atctaattaa caaaaacacc aaaaattggg aattggatcg aattgctttt
     2821 atggatattg ttattatgca agcagctctg gcagaattaa tggaattccc aaccattccg
     2881 gtgaatgtta ctttaaacga atacattgaa atttccaaaa tgtatagcac tgaaaaaagt
     2941 ggcaaattta ttaatggcat cctcgacaat attgtaggag agttaaaaaa agaaaataaa
     3001 ttaataaaag ttatgacatt taagaataat aaaaaataat cgtttatctt tgcattacaa
     3061 ataattatta acttactctt ttttaaaaat ttagcaaatt aatttaacaa taaaagcaaa
     3121 aattatgaat ttactaacta tcttattaca agcaaaacca gccgatcaac caagcggtgg
     3181 aggttatacc ggtattttaa tgctgatttt gatttttgtg gtattttact ttttccttat
     3241 cagaccacaa acaaaacgtc aaaaagaaat aaaaaagcaa cgtgaagcga tgaaaccggg
     3301 tgatcatgta attacttctg gaggaattta cggaaaaatc aaagaagtta aagacaatgc
     3361 tttcattatt gaaattgctg ataacgtgcg aattaaagta gataaaaact ctgtatttct
     3421 tactgctgaa gatattcaga atactccgtc taaataaagt cggaacccta tgttccctaa
     3481 taacttaata aaaatattaa agttttatgg agcaaagatg aaaatgttct ttctctcaaa
     3541 ggatgttttc atctttttat tttttcttgt actttctaca gggttctggt ttattaatgc
     3601 attgagcaag ccacgtgaaa agacaattga agttcctcta caatatgaag gattgccacc
     3661 tgatattgtt attacgaatt ctccaccaaa atttatttct ttaaaaatca gagatgaagg
     3721 cagaaatctt ttgagttata ctcgaaaaaa gctgaatcct ttgataattc aattagcaag
     3781 aaattttcag gaaaatggag aaatcattgt ttctacagat caactcttga ataaattcac
     3841 ttattacctg caacctacta cactcatttt agaagccaag ccagaaacta ttttaattag
     3901 atatgaaaaa ctttatagca aggttttacc tgtagaatgc aaaatcaatt ttgatctttt
     3961 gcctcagtat atgattagcg atgaaataaa attacagccg agtaaagtaa atgtctttgg
     4021 accaaaacac ttattagatt ctctgaaaac tataaaaacc gaacttattt atttaaaaag
     4081 cttgcatgat actactatta taacagctaa acttcaatcg atagcgaatg taagattttt
     4141 acctgaaatt gtcgaagtta ttattcctgt ggaaatgttt acggagaaga aagttcaact
     4201 gccgatcact gtaataaatg ctcctggtga tatttattta cgaaccttac catcaaaagt
     4261 tattgccact ttcaatatcg caatgagtcg ttatcattcg tttaaacctt ctgatattca
     4321 ggtggcagtt gattataatg aagtcaaaaa caataattct gataaattaa atgtaaagat
     4381 tctcagatat cctatttatg tgcaaaacat tcgtttggat cctcaacaag ttgatttttt
     4441 tgttgagaag aataaatgat ttatttatta attcactatc tttaaaataa attattatgt
     4501 tacagcgtat tcagacattg tatttaacaa ttgttcttat actttcctgt cttacacttt
     4561 tttctcccgt tgctaatttg tttaataaag aggatttaac tttttatgaa ataaattatc
     4621 gtggtatttg tctgattgaa gatgattcta aaacacccgt ttcgtcagta tgggctttaa
     4681 cggtcatttc agctattgtt ccattagtta cactggcaac tatttttttt tataaaaaga
     4741 gaatgcttca aatacgtctt tccattttca atctcattat catgattggg tattacgtaa
     4801 tattgttcat ttacatctgg tttgccacca aaaatttacc tgcttcacac tggaatttaa
     4861 aaattgtttc tgcttttccg ctcataaaca ttatactgac aattttagct atgagagcaa
     4921 ttgtcaaaga tgaaagatta attaagtcac taaatcgatt acggtaaacg taaaaaataa
     4981 tttttgaatt gaattttata acgtggaaaa gtgctaattt tttaatttga ggaaaaaata
     5041 tttttcctca aaaaaattaa acaaaccacc ataaaaacaa aacaacaaaa atcaaagcag
     5101 gcagtaaatt aatgattctc agttttttaa tgccaagtat atcaattcct aaacctatta
     5161 gtaaaattcc acccacgctg gttaatcctt gaataatttc cgaagtaaaa aatgaggaag
     5221 cccacatagc cagtaaagtt attccaccct gaaaaattaa caatggaata gcagccacaa
     5281 taaccccagc acctaatgca gaacctaaaa taatagaaga aaaaccatcc attaatgatt
     5341 ttgtaaacaa caagtcagaa gagccatcaa tgccttcctg aatggcacct aaaatagtca
     5401 ttgagccgat gcaaaacaac agaaaagccg tcaccaaacc ttctgaaaag cgttcacctc
     5461 ccaaatgaag ttttcttttc aggtattcac tgaattgttc t
//
LOCUS       DAOTKS010000066         5495 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_13361_length_5495_cov_4.109191, whole genome shotgun sequence.
ACCESSION   DAOTKS010000066 DAOTKS010000000
VERSION     DAOTKS010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5495
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13361_length_5495_cov_4.109191"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="P5236_02190"
     CDS             <1..279
                     /locus_tag="P5236_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013264990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HRU63202.1"
                     /translation="LNLMTHQTNRAACFRTVIVLILEGKKYFFEGKVKGIISEEPRGN
                     GGFGYDPIFIPEGFNKTFAELTAEEKNQISHRALALKRLSDFLEKINH"
     gene            432..2714
                     /locus_tag="P5236_02195"
     CDS             432..2714
                     /locus_tag="P5236_02195"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRU63203.1"
                     /translation="MLKAENLAMGKWRTHLAYNSISRIAQSDDKIYATGDNALFSVDK
                     RDGSIELYSKISGLHDNVVGNFEYDNISKQLLIVYANGNMDIMGSGGIINIPDLYNKP
                     QISDKQINHIYFYGNKAFLSTNFGIIVVNIQKNEIADTYYIGPNASEVKVLATTVSNG
                     YIYALTEDAIYQASVNEPNLVNYQFWTIDNTIPGSGNFQAIVSFAGQLFILRNNKIYK
                     KDSNNLWTTFYSEITATNLSVSNGKMIIYTTTNVYVVDEAMNFKTISGLGKILDVEYD
                     TNNNLYWLAANSQGVVSYQENSGGTPIISTYKPSGPAVNIPWKMRFSGQKLFVVPGGR
                     WSAQNNQPGYVMIYENGLWTNIDGKDIEAQTGKSVLDFMDIAIDPADPSHFYIPSYGT
                     GVYEFKNNQFAAWFNFNNSTIETIFPDNPNSAYLYMRMDGGVFDTEGNVWFTNSLVLN
                     GIKFRKPDGTWSKLTYPSLLSKETLGELMILKQKTQQKWVLSVRSAPGICIFDDNGTL
                     DDQNDDKSIFYSSFQTPDEKITPTSYYCLTQDKNGVVWVGTDMGPLLFYNPTNAFNSS
                     EFMASRVKIPRNDGTNLADYLLADEKVKAIAIDGANRKWLGTESSGVYLMSENGQETI
                     HHFTTLNSPLLSNTILSIAINPVTGEVFFGTGNGIISYQSDAVEAGDTFGDVYAYPNP
                     VRENYNGIITITGLVDKTHVKITDLNGNLICETISNGSIATWDGKDAHGRRVSTGIYL
                     IICTNSEGSQSAITKILVIN"
     gene            3131..4291
                     /locus_tag="P5236_02200"
     CDS             3131..4291
                     /locus_tag="P5236_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63204.1"
                     /translation="MNNLITKNYKYNGKTLLLCIIYLIINGLFIFKYIRRFNFVDSLI
                     VFFIYILLILTFFIINQKLQKKDKLLKFFLISLLIISLALIIFIQIKINPLTLKVDRW
                     SAINNFLSNLFKGKSPYSANTHLGGYGSPFPVWQIFHIPFYLLGDVGLCMIFCFVCLI
                     LCVKWYSNSNKVTFTFLSLMALSPAFWYEVSVRSDLMYNFFVVFIALLIIQKKNFNLQ
                     NNTISLGIVCGLFMSTRLSVFLPLFLYFFYEFWTINLKKKILFIVSGLVTFILSFFPF
                     VLWDYKTLFLTNHNPFILQTRQGFGIDIIIIIALAIYFSFKAKNNFYKIIIFISFSLF
                     ILVAINFLFRLANENFADLLFDSGYDITYFNMSLPFIIAGISLINRAMDADL"
     gene            4426..>5495
                     /locus_tag="P5236_02205"
     CDS             4426..>5495
                     /locus_tag="P5236_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63205.1"
                     /translation="MQNKVTHTLSNCPLFYLKKQWWIFLIFISLFIKIFLLPVKTGDY
                     VVYLEPWINFIKTHGYFSALKYDFYNYTPAYLYILAAIAKIGLNPLFLIKGISVLFEY
                     VTAYFVGKIAWLKFKDNLVIIIALAIVPLLPSVMLNSSYLSQCDAIYASFTVASIYYA
                     LKNKSFLSILMLGIAFSFKLQAVFILPFFFVMLLRGNVKWYHFFLIPTVYLISIFPAW
                     LVGRPFSELISIYFSQADYYRLLTMNFPNLYIWFNNNYYELISKAGIFFTFLFTLFGG
                     IKLSLKRINFTFENWIQLAFLSVTVIPFFLPGMHERYLYLGDVLGVAYLMIFPRKFYL
                     PLAVWVISLYSYIRCSRFNEILP"
BASE COUNT         1790 a          797 c          831 g         2077 t
ORIGIN      
        1 ttgaacttaa tgactcatca aaccaatcga gccgcctgtt ttagaacagt gattgtttta
       61 atattggaag ggaaaaaata tttttttgaa ggaaaagtaa aaggaattat cagtgaagaa
      121 cctcgtggca acggtggttt tggttatgat ccgatattta ttcctgaagg ttttaacaaa
      181 acttttgcag aactgactgc cgaagagaaa aaccagatta gtcatcgagc attggctttg
      241 aaaagactta gtgattttct tgaaaaaatc aatcactaaa tatttttgta gagctatcgc
      301 aaaataaatc aaacaaaaaa ttgtttatac ataataaaac tcattgaaaa ttattcactt
      361 aattagctca tatcattaaa atgaaaaaaa atacaatttt ccttttctta ctattgtcag
      421 ttttttgtaa aatgctaaag gctgagaatt tagctatggg taaatggaga acccatcttg
      481 cttataatag tatttccaga attgctcaat ctgatgataa aatttatgca acaggagata
      541 atgctttatt ttcagtagat aaacgagatg gaagcataga actttatagt aaaatttctg
      601 gacttcatga taatgtagtt ggaaattttg aatatgataa tatttctaaa caattactca
      661 ttgtatatgc caatggcaat atggatatta tgggatcagg tggcattatc aatattcctg
      721 atttatataa taaaccccaa atttcggata aacaaatcaa tcatatttat ttttatggta
      781 ataaagcatt tttatctaca aattttggca tcatcgtagt caatattcag aaaaatgaga
      841 ttgctgatac ttattacatt ggtccaaatg cttcagaagt aaaagttttg gctacaacag
      901 tttctaatgg ttatatttat gctttaacag aagatgctat atatcaggct tctgtcaatg
      961 aaccaaattt agtgaattac caattctgga ccattgacaa tactataccg ggaagtggca
     1021 attttcaagc tattgtttcg tttgccggtc aactttttat tctccgaaat aacaaaattt
     1081 acaaaaaaga tagcaacaat ttatggacaa ctttttattc cgaaattaca gctaccaatt
     1141 tgagcgtttc taatgggaaa atgataattt atactaccac taatgtttat gtagttgatg
     1201 aggcaatgaa ttttaaaaca ataagcggat taggaaaaat tttagatgtg gaatatgata
     1261 ccaacaacaa tttatattgg cttgctgcaa acagtcaagg agtcgtttcc tatcaagaaa
     1321 atagtggtgg aacgcctatt atttctactt ataaaccttc aggaccagcg gtaaatattc
     1381 cctggaaaat gcgtttttcg ggtcagaaac tttttgtagt tcctggtggg cgatggtctg
     1441 ctcaaaataa ccagccaggt tacgttatga tttatgaaaa tggtttatgg acaaacattg
     1501 atggaaaaga tatcgaggct caaactggta aaagcgtgct tgattttatg gatattgcca
     1561 tagacccagc tgatccttct catttttata ttccgtctta tggaacagga gtttatgagt
     1621 ttaaaaataa tcaatttgcg gcttggttta attttaacaa cagtacgatt gaaactattt
     1681 tccctgataa tcctaatagt gcttatcttt atatgcgaat ggatggagga gtttttgata
     1741 cagaaggaaa tgtttggttt acaaattcac tcgtgttaaa tggaataaaa tttagaaaac
     1801 cagatggtac ctggtcaaag ttaacctatc cttctttatt atcaaaagaa acattaggag
     1861 aattaatgat attaaaacaa aaaacgcaac agaaatgggt tttatcagta cgttcagcgc
     1921 ctgggatttg catttttgac gacaatggca cgttggatga ccaaaatgat gataaatcta
     1981 ttttttattc ttctttccaa actcctgatg aaaaaatcac tccaacctct tattactgtc
     2041 ttactcaaga taaaaacggt gtcgtttggg taggaacaga tatgggacct ttgctttttt
     2101 ataatcccac caatgctttt aattcttctg aatttatggc ttctcgcgta aaaattcctc
     2161 gcaacgatgg tactaacctt gcagattatc tgcttgcaga tgaaaaggtg aaagcaattg
     2221 ctattgatgg agccaatcgg aaatggttag gaactgaatc atcaggtgtt tatttaatgt
     2281 ctgaaaatgg acaggaaact attcatcatt ttactacttt aaatagtcct ttattatcca
     2341 ataccattct ttccatcgct attaatcccg taacaggaga agtttttttc ggaacaggca
     2401 atggtattat ttcttatcaa agtgacgctg ttgaagctgg tgatactttt ggcgatgttt
     2461 atgcttaccc caatccagta cgagaaaatt ataatggcat tattactatt actggattgg
     2521 tagataaaac ccacgttaaa attactgatt taaatggtaa tttgatttgt gaaaccatat
     2581 cgaatggcag cattgctacc tgggatggaa aagatgcgca cggacgcaga gtaagcactg
     2641 gaatatatct aattatatgc accaattctg aaggaagtca aagtgcaatt acaaaaattt
     2701 tagtaataaa ttaaataaac gtaaaaaata aaatactttt tgtgaattac tcgaataaaa
     2761 gaaaaattta attgataact attttattaa ctgaatgatg ctttacatga tgtcgttaga
     2821 aatgcattgt ttcatgtcaa taaataacaa ggagtcctta tgtaatatta aattagaaca
     2881 actttttata attttaaaag atatcagaag ttttttaatt atttgataaa tattttctta
     2941 aaagttgtca tatcatttaa aatatttaag cattatccgg aacatttgct taactaatac
     3001 ttaaataagg ctaattgttt tgtgatatcc tcttacctcc tttgttcatt ttctcattat
     3061 taaaattatc tgtattagca tcgatttttt gaaaaaatga aaatcaaaaa aataaatttc
     3121 aatcgaaatt atgaataatt taatcaccaa aaattataaa tataacggta aaacattact
     3181 tctttgcatc atatatctta ttataaatgg tttatttatt tttaaatata taagacgatt
     3241 taattttgtt gattctctta tcgttttttt tatttatatt ttacttatat taactttttt
     3301 cattataaat caaaaactcc agaaaaaaga taaactttta aagttttttc tgatttctct
     3361 tctcataatt agtttggctt taattatttt tattcaaata aaaatcaatc cattaacact
     3421 taaagttgat agatggtcag caattaataa ttttctatct aacttattta aaggtaaatc
     3481 accttactct gctaacactc atttaggagg atatggttct ccattccccg tttggcaaat
     3541 ttttcacata cctttttatt tattaggaga tgtgggactt tgcatgattt tttgttttgt
     3601 ctgtttgatt ttatgtgtaa aatggtattc taattcaaac aaagtaacat ttacttttct
     3661 ttcccttatg gctttatctc ctgctttttg gtatgaagtc tcagtaagaa gcgatctaat
     3721 gtataatttt tttgtggttt ttattgcttt actgattata caaaaaaaga attttaatct
     3781 tcaaaacaat acaattagct tgggaatcgt ttgtggctta tttatgtcta ccagattatc
     3841 tgtatttctt cctttattct tatatttttt ttatgaattc tggacaataa atttaaaaaa
     3901 gaaaatactt tttattgttt caggattggt cacttttatt ttatcttttt tcccctttgt
     3961 tttatgggat tataaaactt tatttttaac taatcataat ccatttatat tacaaacaag
     4021 acaaggtttt ggaattgata ttataattat aatagcatta gcaatatact tttcgtttaa
     4081 agcaaaaaat aatttttata aaattatcat tttcatttct ttttcattat ttatcttagt
     4141 tgcaattaat tttttatttc gattggcgaa tgaaaatttt gcagatttat tgtttgactc
     4201 tggatatgac attacctatt ttaatatgag tttacctttt ataatagctg gtataagctt
     4261 aattaataga gctatggatg ctgatcttta aggctaaata caaatttatg taaaaataaa
     4321 cgatttcgaa ctgatttttt ataccacagt atcttgtctg ctggaaatat tccaacttct
     4381 cttattgaca aacaccttga tatttttgaa taaatgctct taaaaatgca aaataaagtt
     4441 actcatactt tgtcaaattg tcctttgttt tatttgaaaa aacaatggtg gattttcctg
     4501 atttttatca gtttattcat aaaaattttt cttttgccag taaaaaccgg tgattatgtt
     4561 gtatatttag aaccctggat taattttata aaaacgcatg gctacttttc tgccttaaaa
     4621 tatgattttt acaattatac tcctgcttac ttgtatattt tagccgcaat tgctaaaata
     4681 ggattaaatc ctctgtttct tatcaaagga atttctgttt tatttgaata tgtaacagct
     4741 tattttgtgg ggaaaatcgc atggttaaaa tttaaagata atctggtaat tatcattgcg
     4801 ttagcaattg ttcccctgct tccttctgtg atgctcaata gcagttatct ctcgcaatgt
     4861 gatgctattt atgcttcgtt tacagtagcc agcatttatt atgcactaaa aaacaaatct
     4921 tttctttcaa ttttaatgtt aggaatagct ttttcgttta aattacaagc tgtttttatc
     4981 ctgccattct tttttgtgat gctcttacgg ggcaatgtta aatggtatca tttttttctc
     5041 attccaactg tctatcttat tagcattttt ccagcatggt tggttggtag acctttttca
     5101 gaattaataa gcatttattt ttctcaagcc gattattacc gattgcttac catgaatttc
     5161 cccaatcttt atatctggtt caacaataac tattatgaat taatttcaaa agcagggatt
     5221 tttttcactt ttttatttac tttgtttggt ggaataaaat taagcttaaa aagaattaat
     5281 tttacttttg aaaactggat tcagcttgct tttttaagcg taacggtaat accttttttc
     5341 ttgccaggta tgcacgaacg ttatctttat ctgggagatg tgctgggagt agcgtattta
     5401 atgattttcc ctcgtaaatt ttatctgcca ttagccgtct gggtgatttc gctttattct
     5461 tatattcgct gctctcgctt caatgaaatt ctacc
//
LOCUS       DAOTKS010000067         5469 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_13424_length_5469_cov_4.320835, whole genome shotgun sequence.
ACCESSION   DAOTKS010000067 DAOTKS010000000
VERSION     DAOTKS010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5469
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13424_length_5469_cov_4.320835"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(432..1682)
                     /locus_tag="P5236_02210"
     CDS             complement(432..1682)
                     /locus_tag="P5236_02210"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HRU63206.1"
                     /translation="MDTKLNTIEEAIDDIRRGKFVIVVDDEDRENEGDFICAAEKITP
                     EMVNFMITHGRGVVCAPLTEERCKELDLEMQVDINTSIHSTPFTVSVDKLEGCTTGVS
                     AADRAATLRALADPASKPETFGRPGHIFPLRAKSKGVLRRAGHTEAAIDLIRLAGLYP
                     VGALIEIMNEDGTMARLPQLIEIAKRFDLKIVTIEDLIAYRLKTESLIEKGEKVDLPT
                     LYGHFNLIPFRQISNGKEHIALIKGEWQKEEPILVRVHSSCITGDTFGSLRCECGEQL
                     HKAMQMIEKEGKGVIVYMNQEGRGIGLMNKIKAYRLQEQGLDTVDANLHLGFDADERD
                     YGVGAQILKSIGVTKIRLLTNNPVKRIGLEAYGLSIVENVPIEIQPNPYNAFYLETKK
                     KRMGHDLKFIKDESDPNNEKPTNV"
     gene            complement(1714..3180)
                     /locus_tag="P5236_02215"
     CDS             complement(1714..3180)
                     /locus_tag="P5236_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRU63207.1"
                     /translation="MFRVKILHKYVFKHFITLFIMTFFICIFILLMQFLWKHVEDLVG
                     KGIGISVLFEFFNYSVLTLVPMALPLAILLASLMTFGNLGETSELTAMKASGISLFRI
                     MQPLIFFIALVCVGAFYFSNYVLPISQTKLWTLIFSLKQKSPELEIPVGEFYTNIDGI
                     NIYVREKQGKALKNLMIYDFSAGFNNATVMIADSGEINLTEDKKYLLLSLYNGESFEN
                     LKNQRVTDNPASIPYRRETFRTKEILIDYDAQFNRLDESLLRDQHISKNISQLSYSID
                     SVSKVVKNISIQQSNELLNYSSFARERNTVSASVPIEKNKNITLNADSLFLSLNQPSM
                     IKAVEYAISQAQQIKDQVGFNKIILNDPIMYVRRHQVEWHRKFTLSFACLIFFFIGAP
                     LGAIIRKGGLGMPVVISVVMFIIYYIIDNTGYKMAREGLWSTFAGMWLSSFVLLPVGI
                     FLTYKAVKDAPLLDAEEYTKIWNKMNQKLNSWIDKIKI"
     gene            complement(3371..3757)
                     /locus_tag="P5236_02220"
     CDS             complement(3371..3757)
                     /locus_tag="P5236_02220"
                     /inference="COORDINATES: protein motif:HMM:NF039344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HRU63208.1"
                     /translation="MAKNMLQLEYPLKSASISLLWNKIGTPLGLSEWFANEVIVNDNK
                     YLFKWDNYEETAYLLDIKPLMKIRFQWEDEIENNTYFELKIETNEITGEVALVITDFT
                     DNIEEEDVILLWDKQIDELKRKIGMG"
     gene            complement(3859..4347)
                     /gene="cdd"
                     /locus_tag="P5236_02225"
     CDS             complement(3859..4347)
                     /gene="cdd"
                     /locus_tag="P5236_02225"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HRU63209.1"
                     /translation="MYKNNIVIPVTICQFEELSPSYQHLIIKAKEEVKKAYAPYSHFY
                     VGVALELGNGEIITGNNQENSAYPSGLCAERVAIFYANAKYPEVPVKTLAIAAFSNNE
                     FLKKPITPCGACRQVLLETELRFQNDITILLYGSEEIYLIKNVKDLLPLSFDKYQLKG
                     NK"
     gene            4571..>5469
                     /locus_tag="P5236_02230"
     CDS             4571..>5469
                     /locus_tag="P5236_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HRU63210.1"
                     /translation="MKKIKWLLSFILFCGFANSQVQNKAYLTYIEKYHSLAEKQQKLH
                     NIPASIILAQGLLESSAGQSYLSQRANNHFGIKCNNDWTGETIYWDDDEKNECFRRYK
                     SVSESYEDHSLFLKKRQRYAFLFQLDPTDYQGWAFGLKNAGYATDPAYAYKLISIIEN
                     YNLQRFDKAEFKEATIATSDIYRLSAYISHKVFKNNGVPYVVATPCDTYESIAEEFSL
                     EEERLRNYNEVDAEAELIAWEKVYIRPKKNKASKNHPIHVVQEGESMHSIAQEYGIKT
                     EKLYKLNHLPYTEGARVGQVLKL"
BASE COUNT         1900 a          922 c          780 g         1867 t
ORIGIN      
        1 tttcattttc agacaactcg cctttcttga tcagttcttt ggcaaattct tcagcttttt
       61 cgcgggtcaa tgaaataagc cctatgcctg ctaaaactgc atttttgata atttctgaca
      121 taatgcgatc ctcctttatt ttatgagata agtggttaaa aatttttctt tgataaacta
      181 aaaaatgata aatatttttt tactctgaat tttatgtaaa aatacaacat ttttttaaaa
      241 acactattgt ttagaaacaa cttatttttc tttttaaatt cttagaaaca ttagaaacaa
      301 gagaaaagat tgtggaaaat tattatttca tgcaaaaaga gagcactaac ttatgcctat
      361 taattggtca ctttcatctt ataatcaacc ataaattcaa tagccaatct caaaaaagaa
      421 gaaacatatt ctcatacatt agttggcttt tcattatttg gatcagattc gtcttttatg
      481 aattttaaat catgtcccat tcgtttcttt ttagtttcca aatagaaagc attataagga
      541 ttaggttgga tctcgatagg aacattttca acaattgaca atccataagc ttctaaacct
      601 atgcgtttta cagggttatt ggttaaaagt ctgatttttg ttacaccgat tgattttaat
      661 atttgtgctc caacaccata atctctttca tcagcatcaa aacctaaatg taaattagca
      721 tcaactgtat caagtccttg ttcttgcaat ctataagctt taattttatt catcaagcca
      781 atccctcttc cctcctgatt catataaaca atcactccct ttccttcttt ctcaatcatt
      841 tgcattgctt tatgtaactg ttcaccacat tcacaacgta aagaaccaaa agtatccccg
      901 gttatgcaag atgaatgtac tcgtaccaga ataggttctt ctttctgcca ttcacctttt
      961 attaaagcta tatgttcttt tccatttgaa atttgacgga atgggatcaa attaaaatgt
     1021 ccataaaggg taggcaaatc gaccttttct cctttttcaa tcaatgattc agttttgagt
     1081 cgataagcaa tcaaatcttc aatagtcact atttttaaat caaaacgttt agcaatttct
     1141 attaattgag gcaaccgtgc cattgtgcca tcttcattca ttatttcaat aagtgcacct
     1201 actggatata aacctgctaa gcgaattaga tctattgctg cttcagtatg accagctctt
     1261 cgtaaaactc ctttcgattt tgctcgtaaa ggaaaaatat gtccgggacg tccaaaagtt
     1321 tcaggcttgg atgcaggatc tgccaatgct cttaaagtag ctgctctatc ggcagcagaa
     1381 acgccagtag tacaaccttc taatttatct acgcttaccg taaaaggagt ggaatgtatg
     1441 gaagtattaa tatcaacttg catttccaaa tctaattcct tgcatcgttc ttccgtcaaa
     1501 ggagcacaca ctactcctct tccgtgtgta atcataaaat tcaccatttc aggagtaatt
     1561 ttttcagcag cacaaataaa atctccttcg ttttcacgat cttcatcatc aactacaata
     1621 acaaattttc cgcggcggat atcatcaatt gcttcctcaa tggtgtttaa ttttgtgtcc
     1681 ataaataatt atcatttaat cgaatatcaa atcttatatt ttaattttgt ctatccatga
     1741 attcagtttt tgattcattt tattccatat ttttgtatat tcttcagcat ccaggagtgg
     1801 agcatctttt actgctttat aagttaaaaa tatacctact ggtaaaagta caaaagagct
     1861 aagccacatg cccgcaaaag ttgaccatag accttcccgt gccattttat agcctgtatt
     1921 gtcaataata taataaataa taaacattac taccgaaatc accacaggca ttcccaaacc
     1981 acctttacga ataatagctc caagaggtgc tccaatgaag aaaaatatta agcatgcaaa
     2041 agaaagcgta aattttcgat gccattctac ctgatgcctt ctaacataca tgatagggtc
     2101 atttaatata attttattga atccaacttg atcctttatt tgctgagctt gtgaaatggc
     2161 gtattccaca gcttttatca tagaaggctg attcaacgac aaaaaaagtg aatcggcatt
     2221 taaagtgatg tttttgtttt tttctattgg aactgatgct gaaacagtgt ttctttcacg
     2281 agcaaatgaa ctataattta ataattcatt tgattgctga atacttatat tttttacaac
     2341 tttactaaca gaatcaatgg aatacgaaag ttgtgaaatg tttttactga tatgttgatc
     2401 gcgaagtagt gattcatcta aacgattaaa ttgtgcatca taatcaatta aaatttcttt
     2461 cgtcctaaaa gtttctcgtc tgtaaggaat acttgcagga ttatcagtga cacgttgatt
     2521 tttaagattt tcaaaacttt ctccattgta cagagagagc aataaatact ttttatcttc
     2581 tgttaaattt atttcgccag aatctgcaat cattactgtt gcattattaa atccagccga
     2641 aaaatcataa atcattaaat tttttagagc ttttccttgt ttttcacgta catagatatt
     2701 tatgccatct atgtttgtat agaattctcc tactggtatt tccaattcag gtgatttctg
     2761 tttcaatgaa aaaattaatg tccataattt tgtttgagag ataggcaaaa cataatttga
     2821 gaaataaaaa gcaccaacac aaacaagggc gataaagaaa ataagaggct gcatgattct
     2881 aaaaagagaa attccagatg ctttcatagc tgtaagctca gaggtttctc ccagatttcc
     2941 aaaagtcatt agtgaagcga gcaagatggc aagcggtaaa gccatcggca ccaaagttag
     3001 aactgaataa ttgaaaaatt caaataaaac agaaatacct attcctttac ctaccaaatc
     3061 ttctacatgt ttccataaaa actgcatcaa caaaataaag atgcagatga agaaagtcat
     3121 gataaagagc gtgataaaat gtttgaaaac atatttatgt aatattttta ctctgaacat
     3181 aattttttta aaatgcaaaa ttacaccaaa aagttcaata atttaggaag aaaaacatga
     3241 aattaacatt cattgaaaac tataatattt ttatatataa ataaaaaacc atttgttaaa
     3301 tggaaaagtt ctccaaaaca aatggtaatt aaattaattg aagtaaaaat aattttaaaa
     3361 aacaatttta ttaccccata cctattttac gtttcaattc atctatttgc ttatcccaaa
     3421 gcaatataac atcctcttct tcaatattat cagtgaaatc agttattact agcgcaactt
     3481 ctccggttat ttcattggtt tcaattttta gttcgaaata ggtattattt tctatttcat
     3541 cttcccattg aaaacgaatt ttcatcaaag gtttaatatc taacaaataa gctgtttctt
     3601 cataattatc ccacttaaaa agatatttat tatcatttac aattacttca ttggcaaacc
     3661 attcagaaag tcccagcgga gtacctattt tgttccaaag caaacttata gaagctgatt
     3721 taagtggata ttccaattga agcatatttt tcgccatctt ctcttaattt taattcacaa
     3781 gaaaaaaata ctgttagtaa acgagagcca atataaaaat aataattcaa ataaacaagg
     3841 aaaaagaaaa agaagcgatt atttattgcc ttttaattga tatttatcaa aacttaaagg
     3901 aagtaaatct tttacgtttt taattaaata aatttcttcg cttccataaa gcaaaatagt
     3961 aatatcattt tgaaaacgaa gctcagtttc aagcaaaact tgtcggcaag ctccacaagg
     4021 agtgattggt ttttttaaga attcattgtt gctaaaagct gcaattgcta atgttttaac
     4081 gggtacctca ggatattttg catttgcata aaaaattgca actcgttcgg cacataaacc
     4141 tgaaggataa gccgaatttt cttgattatt tcctgtgata atttcgccat tccccagttc
     4201 taaagctact cctacataaa aatgtgaata aggagcatag gcttttttta cctcttcttt
     4261 tgcttttatg atgagatgct gatatgaagg ggacaattct tcaaattgac aaatagtgac
     4321 cggtattaca atattgtttt tatacataca taatttcctt taatgttttt aacttattga
     4381 aaatcaaatg acattaatat taaaatttaa tgccaaatta caaaaatagg aaattaattc
     4441 ttttttaggg cttattttcg gaaaaataaa ttatttttgt atatatcttt acattttgtt
     4501 aagagacact ttatcataat ttaaaataac aaatttgaat atcgatattt gctaactaat
     4561 tgtttttata atgaaaaaaa taaagtggtt attatctttt attttatttt gtggctttgc
     4621 caatagtcaa gtgcaaaaca aggcttatct aacttacatt gaaaaatatc attctctggc
     4681 agaaaaacag caaaaattac ataacattcc tgccagcatc atattagcac aaggattatt
     4741 agaatcgtca gcagggcaaa gttatctctc tcaacgagcc aacaatcatt ttggaataaa
     4801 gtgtaacaat gattggacag gcgaaacaat ttattgggac gatgatgaga aaaatgaatg
     4861 tttcagaaga tataaaagcg tatcagaatc gtacgaggat cattctcttt ttctgaaaaa
     4921 aaggcaacgt tatgcttttt tgtttcaatt agatcctact gattatcaag gatgggcatt
     4981 cggacttaaa aatgcaggtt atgctaccga ccctgcctat gcttacaagt tgatttcaat
     5041 cattgaaaat tataatttgc aaagatttga taaggctgaa tttaaagaag caacaattgc
     5101 aacatctgat atttaccgct taagcgctta tatttctcat aaagttttta aaaacaatgg
     5161 tgttccgtat gtagttgcta ctccatgcga tacttatgaa agtattgcag aagaatttag
     5221 tctggaagag gaacgattgc gaaattataa cgaagtagat gctgaagctg aattaatagc
     5281 atgggaaaaa gtttatattc gtccaaagaa aaacaaagct tcaaaaaatc atcccattca
     5341 cgtagtacaa gaaggtgaat ccatgcatag catagctcaa gaatatggaa taaaaacaga
     5401 aaaactttat aaactcaatc acttacctta tacagaaggg gcacgagtgg gtcaggtttt
     5461 aaaattaag
//
LOCUS       DAOTKS010000068         5431 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_13535_length_5431_cov_3.480283, whole genome shotgun sequence.
ACCESSION   DAOTKS010000068 DAOTKS010000000
VERSION     DAOTKS010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5431
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13535_length_5431_cov_3.480283"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..1805)
                     /gene="ilvB"
                     /locus_tag="P5236_02235"
     CDS             complement(117..1805)
                     /gene="ilvB"
                     /locus_tag="P5236_02235"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HRU63211.1"
                     /translation="MKITGAKALMESLLQEGVNTIFGYPGGAILPVFDALYDYDKEIK
                     HILARHEQGAVHAAEGYARVSGKVGVSIITSGPAATNVITGISDAMLDSVPLVVITGQ
                     VGAGLLGSDAFQEIDVIGITQPITKWAYQIRRAEDVAWAIARAFYIAKSGRPGPVVLD
                     FTKNAQSEEVEFIYKPCKFIRSYIPVPELNPSQIEVAAQIINSAQKPLAIVGHGVIIG
                     EAETELKEFLEKADIPAAWTLLGASAMPTDFRLNMGFIGMHGNVAPNMKTNECDVLIA
                     IGMRFDDRVTGDLNTYAKQAKIIHIDIDAAEINKNVKVDAPVLASVKDALPALTKLIK
                     PAKHQEWIASFKEYADLEFEKVIQREIFPQSGKITMGEVIHKVSEATGKNAICVTDVG
                     QNQMAAARYFGFTKSRSLITSGGLGTMGFGLPAAIGAKIAAPNRTVCLFSGDGGFQMT
                     IQELGTIMEHQIGVKMIVLDNQFLGMVRQWQELFYEERYSFTRMKNPDFVAIGKAYNI
                     DGRWVTEREQLDEAIADMLKDDKPYLLAVKVEKKGMIYPMIPAGTTVTNIILGD"
     gene            complement(1879..3708)
                     /gene="ilvD"
                     /locus_tag="P5236_02240"
     CDS             complement(1879..3708)
                     /gene="ilvD"
                     /locus_tag="P5236_02240"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRU63212.1"
                     /translation="MEMELRSLTSTSGRRMAGARALWRAVGMKDEQMGKPIIAIVNSF
                     SQFVPGHVHLHEIGQKIKAEIEKLGCFAAEFNTIAIDDGIAMGHDGMLYSLPSRDLIA
                     DSVEYMMNAHKADAMICISNCDKITPGMLMAAMRLNIPAVFVSGGPMEAGELDGVQLD
                     LIDAMVQSADESVSDEQIRRIEYAACPTCGSCSGMFTANSMNCLTEAIGLALPGNGTV
                     VATHINREKLFEKAAHLIVENAFKYYRDNDDSVLPRSIATRAAFLNAMTLDIAMGGST
                     NTVLHLLAIANEAEVNFSMDDIDELSRHVPCICKVAPNTQKYHIQDVNRAGGVLGIMA
                     ELNRDGLIDTSVHRVDGLTLEQALAKYDIMLPNVEDEIIQLYKSAPANRFNLTLGSQN
                     FYYEELDRDRINGCIRDLVHAYTRDGGLAILKGNIAEDGCVIKTAGVDESILKFIGPA
                     KVFHSQEAACDGILSGKVQAGDVVVISYEGPKGGPGMQEMLYPTSYIKSRHLGKECAL
                     ITDGRFSGGTSGLSIGHISPEAAAGGNIALIQDGDIIEIDVPQRTIRVKLNDEELHLR
                     REKELSKGKNAFQPINRQRSVSKALKAYASMVSSADKGAIRII"
     gene            4171..>5431
                     /gene="dxs"
                     /locus_tag="P5236_02245"
     CDS             4171..>5431
                     /gene="dxs"
                     /locus_tag="P5236_02245"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HRU63213.1"
                     /translation="MTEKPLQYLPKINSPDDLKKLDKEELKTVCEELRTFIINELSKH
                     PGHLASSLGTIELTVALHYVFNSPYDRIVWDVGHQAYAHKILTGRRDKFHTNRQFKGI
                     SGFPSPKESKYDAFGTGHASTSISAALGMDVASHLKNEKQRKIVAVIGDGALTGGLAF
                     EGLNNASMTKNDLLIILNDNQIAIDPLKGGINQYLVDITTSATYNKIRYKIYLLLKRL
                     DLIDESKKKNIIRFNNALKAILTRRHNLFEGLNIRYFGPVDGHDVEELVRILTEIKNY
                     KGPKVLHCITKKGKGYEPAEKAVTEWHSPGKFDIETGERNISNGGKKEPPLFQDVFGE
                     TLLELAKENNKIVGVSPAMLTGCSMTIMQKEFPDRVFDVGIAEGHAVTFSAGMAKEGL
                     IPFCNVYSSFLQRAYDNIIHDVALQNLH"
BASE COUNT         1716 a         1046 c          875 g         1794 t
ORIGIN      
        1 gactttcatc ttgtcccttc ttttttatct tatcttttgt cggtcagtag tgaaattttt
       61 tatatctatt ttttctattt taaataagca tgaaaataaa acgaatagaa tttatttcaa
      121 tctcctaaga taatattagt aacagttgtt cctgcaggta tcattggata gatcattcct
      181 ttcttttcta cttttactgc caataaatag ggtttatcat cttttagcat atcggctatt
      241 gcttcatcta actgttctcg ttcagtcacc catcttccat ctatattgta agctttccca
      301 attgctacaa agtcaggatt tttcattctg gtaaaggaat atctttcttc atagaataat
      361 tcttgccatt gacgtaccat tcccagaaat tgattgtcta aaacgatcat ttttacacca
      421 atttgatgtt ccataatagt tcccagttcc tggatagtca tctgaaatcc cccatcgcca
      481 gaaaaaaggc aaacagtacg gtttggagca gctatttttg caccaatagc agctggcagt
      541 ccgaaaccca ttgtccctaa tcctccggaa gtaatcaagc ttcgactttt cgtaaatcca
      601 aaataccgag cagccgccat ctggttttgt cccacatcgg taacgcaaat agcatttttc
      661 cctgtagctt ctgaaacttt atgaattact tctcccattg ttattttgcc cgattgaggg
      721 aaaatttcac gttgaataac tttttcaaat tcaagatcag catattcttt aaaagaagca
      781 atccattctt gatgtttggc tggttttatt aattttgtca aagctggcaa tgcatcttta
      841 accgaagcta aaacaggagc atctactttc acatttttgt ttatttcagc agcatcgata
      901 tcaatatgaa taatttttgc ctgttttgca taagtattca aatcgcctgt tactcgatca
      961 tcaaaacgca ttcctatggc aattaaaaca tcacattcat tggttttcat gtttggagca
     1021 acattcccat gcatccctat aaatcccatg tttaaacgaa aatcagtagg catggctgaa
     1081 gcacctaata aggtccatgc cgctggaatg tctgcttttt ccagaaattc ctttaattct
     1141 gtttctgctt ctcctataat tacaccgtgt ccgacaattg ctaacggttt ttgagctgaa
     1201 ttaataattt gtgctgcaac ttcgatttga gaaggattca gttctggaac aggtatgtaa
     1261 ctgcggatga atttgcaagg tttataaata aattctactt cttcagactg agcatttttt
     1321 gtaaaatcaa gaaccactgg tcctggacgt ccacttttgg caatataaaa agcacgagct
     1381 atcgcccacg cgacatcttc agctcttcga atctgataag cccacttggt aataggctga
     1441 gtgattccaa tgacgtcgat ttcctggaaa gcatcgctgc ctaataatcc tgcaccaact
     1501 tgaccggtaa tgactaccag cggaacacta tccagcattg catcgcttat gcccgtaata
     1561 acattggtag ctgcaggacc tgaagtaata atggatacac caactttacc tgatacacga
     1621 gcatagcctt cagcagcatg tactgcaccc tgttcatggc gtgccagaat atgtttgatt
     1681 tccttatcgt aatcatacaa agcatcaaaa actggcaata tcgctcctcc gggataacca
     1741 aaaatagtat ttactccttc ttgcaataat gattccatta atgcttttgc acctgtaatt
     1801 ttcataaatc aatttttatt attcactcct ataagaacaa aggttatttt tttctaatcc
     1861 ttatattttt aaaaagtttc aaatgattct tattgcacct ttatcagcag agctaaccat
     1921 tgaagcataa gcttttaagg ctttagaaac tgaacgttga cggtttattg gttgaaaagc
     1981 atttttacct tttgataatt ctttttcacg acgtaaatgt aattcttcat cgttcaattt
     2041 gactcgtatc gtacgttgag gtacatcgat ttcaataata tctccatctt gaattagtgc
     2101 aatattccct cctgcagcag cttcaggaga aatatgaccg atagataatc ctgatgttcc
     2161 gccagaaaaa cgtccgtcgg taattaaagc acattctttc cccaagtgac gtgatttgat
     2221 ataagacgtt gggtaaagca tttcttgcat tcccggacct ccttttggac cttcataact
     2281 gatcactacc acatcacctg cctgtacttt tcctgataaa attccatcac aagctgcctc
     2341 ttgagaatga aaaactttag caggtccaat aaattttaaa atactttcat ctactcccgc
     2401 tgtttttatt acgcaaccat cttctgcaat atttcctttt agtattgcca atccgccatc
     2461 tctggtataa gcgtgaacta aatctcgaat gcaaccattt attctgtctc tatcaagttc
     2521 ttcataataa aaattttgtg agccaagtgt taaattgaaa cgattggctg gtgcactttt
     2581 ataaagttga ataatttcat cctccacatt tggtaacata atatcgtact ttgctaatgc
     2641 ttgctcgagt gttaaaccat ccactcggtg aacagatgta tcaatcaaac catcacggtt
     2701 gagctctgcc atgattccta acacgcctcc agcacggtta acatcttgta tatgatactt
     2761 ttgcgtatta ggagccactt tacaaataca tggaacatgg cgagaaagct catcaatatc
     2821 atccatcgaa aaattaacct ctgcttcatt ggcaattgct aaaagatgaa ggactgtatt
     2881 ggttgaacct cccatagcaa tatccagggt catagcatta agaaaagctg cacgtgtcgc
     2941 tatggaacga ggcaaaacag aatcatcatt atcgcgataa tatttaaaag cattttcaac
     3001 aattaaatga gctgcctttt caaacaattt ttctcgatta atatgagtcg ctacaactgt
     3061 accatttcct ggcaaagcaa ggccaatagc ttctgttaaa caattcattg aattagcagt
     3121 aaacatacct gaacaagagc cacaagtagg acaagctgca tattcaattc gccgaatttg
     3181 ttcatcagaa acactttcat cagccgattg aaccatagca tcaattaaat caagttgaac
     3241 accatctaat tctcctgctt ccattggtcc gccagaaaca aatacagcgg gtatattcag
     3301 ccgcatggca gccattaaca ttccaggtgt aattttatca caattactaa tacaaatcat
     3361 tgcatcagct ttgtgtgcat tcatcatata ttccacgcta tcagcaatta agtcgcgcga
     3421 aggtaaagaa tacaacatac catcatgccc cattgcaatg ccatcatcaa tggctatggt
     3481 attaaattca gcagcaaaac atcctaattt ttctatttca gctttaattt tttgtcctat
     3541 ctcatgtaaa tgaacatgac caggcacaaa ctggctaaat gaatttacaa tagcaataat
     3601 aggtttaccc atttgttcat ctttcatacc tacagctcgc catagagccc gtgctcctgc
     3661 cattcttcgt ccactggtac ttgttaaact tcgtaattcc atttcaatat ttttttatta
     3721 tctataaaga aaaaccttcc tcattgtttg ggaggaaggt taacattatt ttttgtttat
     3781 gattgtaaac catatagaac catcctcacc tttgattttt caccaaaagt agcacgacga
     3841 ttctattgat atttctattt gctctgaaca ttttctcttt atttctgttt gcaaagataa
     3901 actttgaaat tgaaaaaaca aatagtttac aaaaaaattt tcttttttaa tcaaaagaat
     3961 tcctcgaggc ttgcctcggg gttattcatt attataaaaa aataggttga aagtaaaaaa
     4021 tcatttttag ttaaattgat aacttatgat ttcatataat tgtttataaa aaatttaaac
     4081 gtaaatttgt aataggataa aaaataataa ttattaggta gctaacgaat aaaataatgt
     4141 attatttttg tgggcaattt taaaactata atgacagaaa aacctttaca atatttacca
     4201 aagataaatt ctccagatga tttaaaaaaa cttgataaag aagaacttaa aactgtctgt
     4261 gaagaattaa gaacttttat tatcaatgaa ctttcgaaac atcctggtca tctggcttcc
     4321 agtttaggaa ccattgaact tactgttgcc cttcattacg tttttaacag cccttatgat
     4381 agaatagtgt gggatgtagg tcatcaggct tatgctcata aaatacttac gggccgacgg
     4441 gataaatttc atacaaaccg tcaattcaaa ggtattagtg gtttcccgag tccaaaagaa
     4501 agtaaatatg atgctttcgg aactggtcat gcttccactt ctatttctgc agcattagga
     4561 atggatgtgg cttcacattt aaaaaacgaa aaacaaagaa aaattgtagc agttattggt
     4621 gatggagctt taacaggggg attggctttt gaaggattga ataatgcttc catgactaaa
     4681 aatgatttat tgattatcct taatgataat caaattgcta ttgatccttt gaaaggtgga
     4741 ataaatcaat atttagtaga tattactact tctgctacct ataataaaat tcgatataaa
     4801 atttatctac ttttaaaaag attagattta attgatgaat cgaaaaagaa aaacattatt
     4861 cgatttaata atgctttaaa agctattcta actcgacgac ataatttatt tgaaggttta
     4921 aacatacgtt actttggtcc tgttgacggg catgatgtgg aagaattggt tcggatttta
     4981 acagaaataa aaaactacaa aggtcctaaa gtattgcatt gcattactaa gaaagggaaa
     5041 ggatacgaac ctgcagaaaa ggctgtaacc gaatggcatt ctcctggaaa atttgatatt
     5101 gaaacaggtg aaagaaatat atcaaatgga gggaaaaaag aaccaccact tttccaagat
     5161 gtttttggtg aaactttatt agaattagct aaagagaata ataagattgt aggcgtttct
     5221 ccggctatgc ttacgggatg ttctatgacc attatgcaaa aggaatttcc tgatagagtt
     5281 tttgatgtgg gaatagcaga gggtcatgcg gtaacttttt cagcaggaat ggccaaagaa
     5341 ggattgattc cgttttgtaa tgtttattca tcttttttgc aaagagctta cgataatatt
     5401 attcatgatg ttgctcttca aaatttgcat g
//
LOCUS       DAOTKS010000069         5393 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_13642_length_5393_cov_4.790933, whole genome shotgun sequence.
ACCESSION   DAOTKS010000069 DAOTKS010000000
VERSION     DAOTKS010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13642_length_5393_cov_4.790933"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..1055
                     /locus_tag="P5236_02250"
     CDS             219..1055
                     /locus_tag="P5236_02250"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HRU63214.1"
                     /translation="MVLGNIVSYFSNDTLILSQNSISRIEDSVSKINLADTDLLSRRL
                     STKIDTIHAINHLEGFEGILHPSSPQTENWVIVLLIVLLSILLVFLYQHPSFITENIQ
                     NFFKIKIDKYRTNIGTSYSFFYSFIFLFSIGVFSLYAYILFYNPAEGFLFSRYFYFCG
                     ITLAFFILKYVFIQLIGFVFFEPNIFTVLKANYFNTFYFFNIILFFLVILQIYFPVYD
                     IKWTLYFALILGILALTSITTKLLKIFSHKKFVYFYILLYLCTLEFLPLIALFRAYQL
                     MY"
     gene            1082..1858
                     /locus_tag="P5236_02255"
     CDS             1082..1858
                     /locus_tag="P5236_02255"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HRU63215.1"
                     /translation="MKIKKILISQPQPNSEKSPYFAISEKYNVKIDFRPLIKVEPLSA
                     KDFRAQHISILDHTAIIFNARHGIDHFFRLCEEMRITVPETMKYFCVSETVALYLQRY
                     IQYRKRKVFFSPTGKLSDLVTLMKKHPDEKYLFVTSDVPNEENMSILVNSNLNFDKAI
                     MYRTVSNDFDPEEEFNYDMIIFFSPVGVSSLLKNFPDFVQGDIQIGCFGASTAQAVRD
                     AGLRLDLEVPQPGFPSMTMALDNFLKEFSKKENNNKKATK"
     gene            1938..2342
                     /locus_tag="P5236_02260"
     CDS             1938..2342
                     /locus_tag="P5236_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRU63216.1"
                     /translation="MKNRQFSFPKSERLHSKIKINRLFTEGKSFIVYPLKIFYNFEPS
                     EEFNVCILISVPKKQFKKAIHRNRIKRLLRETYRLNKHLIFEKFSQRKFQIQLAFVYV
                     ANEEIDFYKLNEKMKIALQKLIELVDHDKESY"
     gene            2326..2547
                     /gene="yidD"
                     /locus_tag="P5236_02265"
     CDS             2326..2547
                     /gene="yidD"
                     /locus_tag="P5236_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005655675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRU63217.1"
                     /translation="MKKAIEFIVLLPVYIYRYTISPLFPPSCRYTPTCSQYAIEAVKK
                     HGIFKGGWLAVKRLSRCHPWGESGYDPVP"
     gene            2643..>3429
                     /gene="lpxK"
                     /locus_tag="P5236_02270"
     CDS             2643..>3429
                     /gene="lpxK"
                     /locus_tag="P5236_02270"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HRU63218.1"
                     /translation="MKNQFIHIILYPFAVFYGLAVGIRNWLYDRGIFHSTAFSIPIIG
                     VGNLSVGGTGKTPHTEFILSYVQDEWKTAFLSRGYKRKTKGFFLADEKSTYQTIGDEP
                     YQIYRKFPKVIVAVDEKRVDGVMKLQKQFPDLQLVVMDDAFQHRQIKIGLTILLTDYF
                     HLYTRDRLLPTGTLREQKSGSKRADIVIVTKCPPDLKPIDMRLVEKELNLTGRQQLFF
                     STYEYEEIVPVFPEKEEKKYSLQQINEKNGSLLIVTGIASPEKM"
     assembly_gap    3430..3529
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3530..3886
                     /locus_tag="P5236_02275"
     CDS             <3530..3886
                     /locus_tag="P5236_02275"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HRU63219.1"
                     /translation="EKNGSLLIVTGIASPEKMIEYLRQFSNLIESLIFPDHHDFSKKD
                     IEKMEQKFAQLPANEKIVIVTEKDAVRLLNNAFLSEFLKSKIYYLPIKVKILNHQEDS
                     FIQKIKNYVRKNSKSN"
     gene            3858..4670
                     /locus_tag="P5236_02280"
     CDS             3858..4670
                     /locus_tag="P5236_02280"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HRU63220.1"
                     /translation="MLEKIQKATDFLSAKITTSPKTGIILGTGLGNLITQMDISLEIP
                     YETIPHFPVSTVEGHQGKLIVGKLGGVEVIAMQGRFHYYEGYDMKQVTFPVRVMYALG
                     IRNLLVSNAAGGMNPDFEIGDIMIITDHINLFPEHPLRGKNYDELGPRFPDMSEAYSK
                     DLIAKAKQIAVRNNIKVVEGVYIGTSGPTFETPAEYRYFRTIGGDAVGMSTVPEIIVA
                     NHAGMKCFGISIITDLGVPGKIVEVSHEEVQRIGNSVQPLMTLIMKELVKEI"
     gene            complement(4738..>5393)
                     /locus_tag="P5236_02285"
     CDS             complement(4738..>5393)
                     /locus_tag="P5236_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63221.1"
                     /translation="REFFMLEYRKKIGWDAYLPAEGMLIWHIDYDPTAWDENTVNNYS
                     GTSQTADSHMRVYLQPLSGSTTTPGTAFTSGSFTPITWTGVNINRPITQITKSDDKIT
                     FKLMGGAGEPVIEIGKVDQQLKFARIPVNTQSKKFLNIKTTDLTGDLTLSISGEQASY
                     FLISTSSISKEAANSAEGTNITITYQPTSAGIHTATFTISGGGLDPAKIITLTGEAY"
BASE COUNT         1753 a          775 c          885 g         1880 t
ORIGIN      
        1 agagaccaaa gtaaagcatt tattccatca gggcaaaggg gaaatgctct tttttctcct
       61 ttgtcttgaa aaataatttt ttctttacct tttatttccc ctgaaaaagt tgcattaatt
      121 acttgaattt acgtaatttt atcataggag taaagcattc taatgataat gaaagtgaat
      181 gaagaagtaa tttatttagt gcgaaattaa atgtttttat ggttttagga aatattgttt
      241 cttatttttc taatgatacg ttgattttgt ctcaaaattc tattagtaga atagaagact
      301 ctgtatcaaa aattaaccta gcagatactg atttattatc cagaaggctt tcgacaaaaa
      361 ttgatacgat tcatgcgatt aatcatttgg aaggttttga aggtattttg catccttcct
      421 ctccgcaaac tgaaaattgg gtgatagttt tattgattgt tcttttaagt atattattgg
      481 tttttttata ccagcatcct tcctttatta ctgaaaacat tcaaaatttt tttaagatta
      541 aaattgataa atatcgaacc aatattggaa ccagttattc ttttttttat tcttttattt
      601 ttcttttttc tattggcgta tttagtttat atgcgtatat tctcttttac aaccccgcag
      661 aaggtttttt gtttagtcgc tatttttatt tttgtggcat cacgttggca ttttttatcc
      721 tcaaatatgt ttttattcaa ttaattggtt tcgtcttttt tgaacctaac atttttacag
      781 tattaaaagc taactatttt aacacatttt atttttttaa tatcatttta ttttttctgg
      841 ttattttaca aatttatttt cctgtgtatg atataaaatg gactttatat tttgcattaa
      901 tattgggtat attagcttta acaagcatta caaccaagtt attaaaaata ttttctcaca
      961 aaaaattcgt ttatttctat attttgttgt atctttgcac acttgaattt ttaccattaa
     1021 ttgcgctctt tagagcttat caattaatgt attaagtgtt ttatttttaa acaataaagc
     1081 cttgaaaata aaaaagatac ttatttctca gccgcagcct aatagtgaaa aatcacctta
     1141 ttttgctatt agtgaaaaat acaatgtaaa aatagatttt agacctctta tcaaagttga
     1201 gcctctttct gcaaaagatt ttagagccca acatatttct attctggatc atactgccat
     1261 tatttttaat gcaaggcatg gaattgatca ttttttcaga ttatgtgaag aaatgcgcat
     1321 tacggtacct gaaacaatga aatatttctg tgtttcagaa acagttgctt tgtatttaca
     1381 acgctatatc caatatcgga aaagaaaggt cttttttagt ccgacaggta agttgagtga
     1441 tttagtgacc ctcatgaaaa agcaccctga cgaaaagtat ttatttgtta cttctgatgt
     1501 gccgaacgaa gaaaacatgt cgatattggt caattcaaat ttaaatttcg ataaagcgat
     1561 aatgtatcga acagtgagca atgattttga tcccgaagaa gaatttaatt atgatatgat
     1621 tatttttttc agccctgtgg gagtaagttc attgttaaag aatttccctg attttgttca
     1681 aggtgatatt cagattggat gttttggagc aagtacggct caagccgttc gtgatgctgg
     1741 cttacgctta gatcttgagg tgccccagcc aggttttcct tccatgacta tggcattgga
     1801 taattttctg aaagaattct ccaaaaaaga aaacaacaat aaaaaggcta caaaataaag
     1861 gaagcattag tggatgttaa tattatccca aaagtttaag tatttttctt acttttggga
     1921 taatttattt tattgaagtg aaaaatcgac aattttcatt ccctaaatca gaacgattgc
     1981 atagtaaaat taaaattaat cgtttattta ctgaaggtaa aagttttatt gtttatcctt
     2041 tgaaaatttt ttataatttt gaaccgtcag aagaatttaa tgtttgtata ttaataagtg
     2101 ttcccaaaaa acaatttaaa aaagctattc atcgaaatag aataaaacgc ttattaagag
     2161 aaacttacag gttgaataaa catttgattt ttgaaaagtt ttctcaaaga aaatttcaga
     2221 ttcaactggc atttgtttat gttgctaatg aagaaataga tttttacaaa ctgaatgaaa
     2281 aaatgaaaat tgcactacag aaattaattg aattggttga tcatgataaa gaaagctatt
     2341 gaatttattg ttttattgcc tgtgtatatt tatcgataca ctatttcgcc acttttccca
     2401 cccagttgca gatatacacc aacttgctcg caatatgcta ttgaagctgt aaaaaaacat
     2461 gggattttta agggaggttg gttagctgta aaacgactta gccgctgtca tccgtggggt
     2521 gaaagcggtt atgatccagt tccttaacat ttaatttttc aaaagacaaa aagttctttt
     2581 atttgaaaaa agaatcattt tatttttact ccttttgaat tttttccaac aaaacaaact
     2641 ttttgaaaaa tcaatttatc catattattc tttatccatt tgctgttttt tatggcttag
     2701 ctgttggaat acgtaactgg ctttacgata gaggaatttt tcattctaca gctttttcaa
     2761 ttcctattat aggggttgga aatctttcgg taggcggaac aggaaaaact ccgcatactg
     2821 aattcattct ttcttatgtg caagatgaat ggaaaacagc ttttttaagt agaggctaca
     2881 aacgaaaaac aaaaggattt tttctggcag atgaaaaatc aacgtatcaa accattgggg
     2941 atgaacctta tcaaatttat cgcaagtttc caaaagtcat tgtggctgtg gatgaaaaaa
     3001 gggtagacgg tgtaatgaaa cttcaaaaac aatttcctga tcttcaactg gtcgtaatgg
     3061 atgatgcctt tcaacacagg caaattaaaa tcgggttaac catcttgttg acggattatt
     3121 ttcatcttta tacacgcgat aggcttttgc caacaggaac acttcgtgaa cagaaaagtg
     3181 gaagtaaaag agcagatata gtaatagtta ccaaatgtcc tcccgattta aagcctattg
     3241 atatgcgttt agttgaaaaa gaattgaatt taactggtag acagcaattg tttttttcaa
     3301 cttatgaata tgaagaaatt gttcctgtat ttcctgaaaa agaagaaaaa aaatattcac
     3361 tccagcaaat aaatgaaaaa aatggcagtt tattaatagt gacaggaatt gcttcacctg
     3421 aaaagatgan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng aaaaaaatgg
     3541 cagtttatta atagtgacag gaattgcttc acctgaaaag atgatagaat atttaagaca
     3601 attttcaaat ttaatagaaa gcttgatttt ccctgatcat catgattttt caaaaaaaga
     3661 tattgaaaaa atggagcaaa aatttgctca acttcctgca aatgagaaaa ttgttatcgt
     3721 tacagaaaaa gatgccgttc gtttattaaa taatgctttt ttatctgaat ttttaaaatc
     3781 aaaaatatat tatttaccga ttaaggttaa aattttaaat caccaagaag attcatttat
     3841 tcaaaagatt aaaaactatg ttagaaaaaa ttcaaaaagc aactgatttc ctatcggcta
     3901 aaattacaac ttctccaaaa acagggatta ttttaggtac aggtttagga aatttaatta
     3961 ctcaaatgga tatttctttg gaaatacctt atgaaactat tcctcatttt cctgtttcaa
     4021 cagtagaagg acatcaaggc aagttgattg ttgggaagtt aggtggtgtg gaagtaattg
     4081 ccatgcaagg gcgttttcat tattatgaag gttatgatat gaaacaagta actttccctg
     4141 tccgggtgat gtatgcttta ggtattcgta atttattggt atccaatgca gcaggtggta
     4201 tgaatcctga ttttgagatc ggtgatataa tgattataac cgatcatatc aatctttttc
     4261 ctgaacatcc tttgcgaggt aaaaattatg atgaattagg accgcgtttc cccgacatga
     4321 gcgaagctta ttcaaaagat ttgattgcca aagccaaaca aattgcagtt aggaataata
     4381 ttaaagtagt agaaggtgtt tacattggaa ccagtggtcc tacttttgaa acgccagctg
     4441 aatatcgtta ttttcgtact attggcggcg atgctgtagg catgagcact gttcctgaaa
     4501 taatcgtagc caatcatgct ggtatgaaat gttttggtat ttccattatt accgatctgg
     4561 gcgttcctgg caaaattgta gaagtttcac atgaagaagt acaacgaatt ggcaactcag
     4621 ttcaaccact tatgacatta atcatgaaag agctggtaaa ggaaatataa tttcatttgt
     4681 tcaattaaaa caaaaaagga acgataccca aaaaattatc gttcctttta ttttttatca
     4741 ataagcttca ccagtcaagg taataatttt tgcaggatcc aatccgccgc ctgagatggt
     4801 aaaggttgct gtatgtattc ctgctgaagt tggttgataa gtaatggtta tgttagtacc
     4861 ttcagcagaa tttgcagctt cttttgaaat acttgaagta gaaatcaaaa aataacttgc
     4921 ttgttcacct gaaatagaaa gtgttaaatc gcctgttaaa tcagtagttt taatatttaa
     4981 aaatttcttg ctttgtgtat tgactggaat acgagcaaat tttaattgtt gatcaacttt
     5041 tcctatttcg ataacaggtt caccagcacc acccattaat ttaaaagtta ttttatcatc
     5101 actttttgtg atttgtgtga taggcctatt aatattcaca cctgtccaag taattggagt
     5161 aaatgaaccg ctcgtaaaag ctgtgccagg tgttgttgta gaaccactta atggttgcaa
     5221 ataaacacgc atgtggctat cggcagtttg tgaagttcct gaataattgt taacggtatt
     5281 ttcgtcccat gcagttggat cgtaatcaat atgccagatt aacattcctt cagcaggaag
     5341 ataagcatcc cagcctattt ttttacggta ttctaacata aaaaattcgc gag
//
LOCUS       DAOTKS010000070         5366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_13709_length_5366_cov_5.270006, whole genome shotgun sequence.
ACCESSION   DAOTKS010000070 DAOTKS010000000
VERSION     DAOTKS010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13709_length_5366_cov_5.270006"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1573)
                     /locus_tag="P5236_02290"
     CDS             complement(<1..1573)
                     /locus_tag="P5236_02290"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HRU63222.1"
                     /translation="MQTCHQQHISFICQYLLLFLGGLVPLLCYSQDKISSSPSFNKDI
                     STDSVLVKTDSTKISSQKQKASPIDAEINYSAKDSIVFYGDGTGYLHGEGEIKYKNIT
                     LKAEFVRVKLDSSLIYARGKVDSLGNKIGEPIFSEGEANYASKELTYNLKSQKGYIKN
                     VVTQQGEGYIIGEKTKKVSEDIFCIADGKYTTCDKFDSPDFYLYLTKGKVKPGDYIVT
                     GPAYLVLADVPLPIAVPFGFFPFTEKYSSGILMPNYEDNISRGFGLTNGGYYFALSDY
                     ADLELLGDIYTKGTWSLTANSRYIKKYKFSGNFSFSYRVDVTGEKAMPDYNKATNMSI
                     RWSHSQDQKANPYFTFSSSVNFSTSGYNRSNINYYYRPDLNSENTKSSSVSFTKRFPG
                     IPNLNLSGSMLISQRTKDSTISLSFPNLNVSVSRFVPFKRKNAIGKEKWYEKIYMSYS
                     GTFANSINTKENLLLSSSFSKDWKNGMKHSIPISATFNVLNYINISPTFNYTERWYLQ
                     SIHKDWDTENQKVKID"
     gene            1859..3097
                     /locus_tag="P5236_02295"
     CDS             1859..3097
                     /locus_tag="P5236_02295"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005790917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRU63223.1"
                     /translation="MKIKKIKRNIFYNFKKLLIVNSFFFPAFLNSFISINAQDNRFIV
                     VLDAGHGGEDPGAKGKISNEKDINLAVVKELGKMMEENKELKVIYTRKTDKYLTLQQR
                     ADIANQNHADLFLCIHTNASKNRSAFGAETYTLGLAKTQSNLEVAMRENSVILLEKDY
                     KTKYEGFDPTSVDSYIMFEFMQDKYLDKSIELASLIQTHFNYHGRLDRGVRQAGFWVL
                     HRSACPSVLVELGFISNPEEELFLNSKEGQRKMAEAIYKAFLEYKREHDKRNGISNNF
                     VNTRVETKTDVKQNIPTDISPNQNIAKIEITDSNIVSASPVPLSDNLLDDQAIVFKIQ
                     IFTSRKKLPPNAPDFKGIKEVAFYEEDGLYKYTVGAESDYNKILNLRNEIKTKFPEAF
                     IIAFKGNSKISVKDALKTSK"
     gene            3164..4072
                     /locus_tag="P5236_02300"
     CDS             3164..4072
                     /locus_tag="P5236_02300"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRU63224.1"
                     /translation="MKKNFFNKEVKIGLMVIIAIFLLYFGINFLKGIDIFSPTRSYYS
                     VYEKLDGLETSSPVFIKGYKVGQVDKIQYDFSQQKPFVVKISIEKNIRLPEGTKIELF
                     DNGLMGGKALQLILNQSDDEHYFQPGDTIPSQISVGLMGQITNSLLPKIENISSHADS
                     LLTSLRKLSESEKINNSLTSLEETSANLAHSSKQLKNIMNNEIPVIIDNVNTLTTDFK
                     AVSNNIKGIDFASTVGNIDQTINNLNLMSDKINNGEGTLGLLLTDKELYFNLANTTGS
                     ANNLLIDLKNNPKRYVHFSVFGSKTK"
     gene            4489..>5366
                     /locus_tag="P5236_02305"
     CDS             4489..>5366
                     /locus_tag="P5236_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HRU63225.1"
                     /translation="MKIHEQLWNRCLKVIKDNINDASFNTWFAPFIPLQYENNCIILQ
                     VPSQFFVEYIEEKYIDLLQMTLYRVIGEGTHLEYRVMIDQSNGKSTQIPSTGEKAKIK
                     LSSSNTTYKKQSLPDIDPQLNLNYNFDTLIEGKSNKLARTAGISIGENPGKTAFNPLF
                     VYGPSGIGKTHLANAIGIKAKQSHADKRVLYVSANTFQIQYTDSVRNNTVNDFLNFYQ
                     TIDVLIIDDIQEFAGKTGTQNTFFHIFNHLHQSGKQLVLTSDRSPLLLAGLEQRLLTR
                     FKWGLSAEIEKPDFELR"
BASE COUNT         1953 a          806 c          793 g         1814 t
ORIGIN      
        1 tatcaatttt tactttttga ttttcagtat cccagtcttt atggattgat tgtaaatacc
       61 atctttcagt ataattaaac gtcggagaaa tattgatgta gtttaaaaca ttaaaggtag
      121 cactaatagg aatagaatgt ttcattccat ttttccaatc ttttgaaaag gaagatgata
      181 ataataaatt ttctttcgta ttgatgctgt ttgcaaaagt tccactgtat gacatataga
      241 ttttttcata ccatttttct ttaccaatag catttttacg cttaaaaggt acaaatctgc
      301 tgacactgac atttaaatta ggaaatgata agctgatggt tgaatcttta gttcgttggc
      361 taattaacat gcttccagat aaatttaaat taggaatacc tggaaatcgc ttggtaaaag
      421 aaacacttga actttttgtg ttttcagaat ttaaatcggg tcgataataa taattaatgt
      481 tgcttctgtt gtaaccactg gttgaaaaat ttacactaga tgaaaaggta aaatatggat
      541 ttgccttttg atcttgagaa tgtgaccatc gaatgctcat gttagtagct ttattataat
      601 caggcatagc tttttcgcct gttacatcca cacgataact gaaactaaaa tttccactga
      661 atttatattt tttaatatat cgtgaattgg ctgtcaatga ccaggttcct ttagtataaa
      721 tatcacctaa aagctccaaa tcggcgtaat cacttaatgc aaaataatat cctccattag
      781 tcaacccaaa gcctcttgaa atattatctt cataatttgg cattaaaata cctgaagaat
      841 atttttcagt gaaaggaaaa aagccaaatg gtacagctat cggcaaagga acgtctgcca
      901 gcactaaata agcaggaccg gtaacgatat aatcaccagg tttcactttc cctttggtga
      961 gatacaaata aaaatcgggt gaatcaaatt tatcacaagt agtgtatttt ccatcagcaa
     1021 tacaaaaaat atcttccgag actttttttg ttttttctcc gataatataa ccttcacctt
     1081 gctgagttac aacattttta atatatccct tttgactttt taaattataa gtcaactctt
     1141 tggaagcata gttcgcttct ccttctgaaa atataggttc tccaatttta ttgcccaggc
     1201 tatcaacttt gccccgagca taaatcaaac tactatctaa tttaacgcga acaaattcgg
     1261 ctttcaaggt tatattttta tattttattt caccttctcc atgcaaatat cctgtgccat
     1321 caccataaaa cacaatagaa tcttttgcag aataattaat ttctgcatca attgggcttg
     1381 ctttttgctt ttgagaagaa atttttgtag aatccgtttt aactaaaact gaatcagtag
     1441 aaatatcttt attgaacgaa ggcgaagaag aaattttatc ttgactataa catagtaaag
     1501 gaacgagccc tcctaaaaat aaaagtaagt actggcaaat gaatgagata tgttgttggt
     1561 ggcaagtttg catccttaat tttatctcta cctaaaagtc aaaaaataac aaatttacac
     1621 gtttgcaaat ttaaataaaa aatatgtcct tcttttacta ttaaatttta atttaggatt
     1681 aatttggttt tttattgttt tttgttttta ttgaaaacac aagaaaaaaa gattattttt
     1741 gcaaaaggaa agtgctttac aattttttat tttaagttaa taaaaaagat aaaatgattt
     1801 attcgttttt gagaaaaaca actctgtata aataaaaata ttttcagggg atttaataat
     1861 gaaaatcaaa aaaataaaaa ggaacatatt ttataatttt aaaaagttac tgattgtcaa
     1921 ttcttttttc tttcctgcct ttttaaatag ttttatttct atcaatgctc aggataatag
     1981 atttatagta gttttggatg ctggacatgg aggtgaagat cctggagcaa aaggaaaaat
     2041 ttcaaatgaa aaagatatta atttagcagt ggtgaaagaa cttggtaaaa tgatggaaga
     2101 gaataaagaa ttaaaagtaa tctatactcg taaaacagat aaatatttaa ccttgcagca
     2161 gagagctgat attgccaatc aaaatcatgc agacttattt ttatgtattc atactaatgc
     2221 ttcaaaaaat cgttctgctt tcggagcaga aacttatact ttgggacttg ctaaaaccca
     2281 atctaactta gaagttgcca tgcgtgaaaa ctctgtaatt cttcttgaaa aagattacaa
     2341 aactaaatat gaaggattcg atcctacttc tgttgattct tatatcatgt ttgaatttat
     2401 gcaagataaa tatcttgaca aaagtattga acttgcctcc cttattcaaa ctcattttaa
     2461 ttatcatggt agattggata gaggtgttcg tcaggcaggt ttctgggttt tgcatcgttc
     2521 agcatgtccc agtgtccttg ttgaacttgg attcatttct aatcctgaag aagaattatt
     2581 cctaaattca aaagaaggac aacgaaaaat ggctgaagct atttataaag cttttctcga
     2641 atataagcgt gaacatgata aacgaaatgg aatttcaaat aattttgtaa atacaagagt
     2701 agaaacaaaa acagacgtga agcaaaatat accaaccgat atttctccca atcaaaacat
     2761 tgcaaaaata gaaataacag attcaaatat tgtttcagct tcccctgttc cactttcaga
     2821 taatttgctt gacgatcaag ctattgtctt taaaatacaa atttttacaa gtagaaaaaa
     2881 acttcctccc aatgctccag attttaaagg aattaaagag gttgcctttt acgaggaaga
     2941 tggattatat aaatatactg tcggcgcaga atctgactat aacaaaatac tgaatttacg
     3001 aaatgaaatt aagaccaagt ttccagaagc attcataatt gcttttaaag ggaacagcaa
     3061 aatttctgtc aaagacgctt taaaaacttc aaaataaaat attttcattt ctctatttat
     3121 aagttgctaa ttaagaacgg tttaaacttt aaaaaacttg ataatgaaga aaaatttttt
     3181 taataaggaa gttaaaattg gattaatggt aataattgcc atttttttac tgtatttcgg
     3241 aatcaatttt ttgaaaggaa ttgatatttt ttcacctacg aggtcttatt atagtgttta
     3301 tgaaaagtta gatggtttgg aaacttcctc tcccgttttt atcaaaggct ataaggtagg
     3361 acaggtggac aaaatccaat atgatttttc tcaacaaaaa ccatttgttg ttaaaatttc
     3421 gattgaaaaa aacattcgtt tgcccgaagg aacaaaaatt gaattgtttg acaatggctt
     3481 aatgggagga aaagctttgc aattaatttt aaatcaatca gatgacgaac attattttca
     3541 acctggcgac acgattcctt ctcaaataag cgttggatta atgggtcaaa ttacaaacag
     3601 tcttttgcca aaaattgaaa atatttcatc tcatgcagat tcattattga cctctctcag
     3661 aaaactttct gaaagtgaaa aaattaataa ttctttaact tctctcgaag aaacttctgc
     3721 taatcttgca cacagttcca aacaactaaa aaatataatg aataatgaaa ttccagtaat
     3781 aattgataat gtaaatacac ttacaaccga ttttaaagca gtgagcaata atattaaagg
     3841 aattgatttt gcttcaacag tcggtaatat tgatcaaacc atcaataatt taaatttaat
     3901 gtctgacaag ataaataatg gcgaaggcac gctgggattg ttattgaccg ataaagagct
     3961 ttattttaat ctggcaaata ccactggaag cgctaataat ttattaatcg atttaaaaaa
     4021 caacccaaaa cgatatgttc atttttcagt tttcggaagt aaaacaaaat aaagttattt
     4081 agaatttgtc taaataagaa agaaatgaaa aaaataaata attacctttt attatcttta
     4141 aaacgcacat taataattta ttcaacttga ttttaataaa aaattatagt ttttcacact
     4201 aaattgttaa catcttttaa atcaggtact tctatataaa ttttgtaaca aactatttga
     4261 atctcaaata attttacaaa catactgatt ataagtaaaa taaaattttg atttttcttc
     4321 cttttaaaaa atcattttga tttttcaaaa taaattaaga tttttgctaa aaggagttga
     4381 agttatcttt acttaaaaaa tttaaatgaa ttacgcgtaa ttcatttatt ttttctgtgt
     4441 caatacaaaa ttcgtaagtt atataacgcg ataatttaat tttttgaaat gaagattcat
     4501 gaacaattgt ggaatagatg cttaaaagtc attaaggata acatcaacga cgcatcattt
     4561 aatacctggt ttgctccttt tatcccattg caatatgaaa ataattgtat tattttacaa
     4621 gtaccgagtc aattttttgt agaatacata gaagaaaaat acatcgactt attgcaaatg
     4681 acactttatc gcgtgatagg tgaaggtaca cacctggaat atcgtgtaat gattgaccaa
     4741 tcaaatggta aaagcacaca aattccaagc acaggtgaaa aagcaaaaat caaattgtct
     4801 tcaagtaata caacttacaa aaaacaatca ttacccgata tcgatcccca attaaattta
     4861 aactacaatt ttgatacgtt aattgaagga aaaagtaaca aattagctcg aactgctggt
     4921 atcagtattg gagaaaatcc tggaaaaaca gcctttaatc cattatttgt atatggacct
     4981 tcgggaattg ggaaaactca tttggcaaat gccatcggca ttaaagcaaa gcaaagtcac
     5041 gctgataaac gagttttgta tgtttcagca aacacttttc aaatacaata tactgattct
     5101 gtacgaaata atacagttaa tgatttcttg aatttctatc aaactatcga tgtattaatc
     5161 attgacgaca tacaggaatt tgctggaaaa actggtacgc aaaatacatt tttccacatt
     5221 tttaaccatt tgcatcaatc agggaaacaa ttggtgctga cttctgaccg ttcacctttg
     5281 ttgctggcag ggcttgaaca acgtttgcta acaaggttca aatggggact ttcagccgaa
     5341 attgaaaaac ctgattttga acttcg
//
LOCUS       DAOTKS010000071         5361 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_13720_length_5361_cov_3.962684, whole genome shotgun sequence.
ACCESSION   DAOTKS010000071 DAOTKS010000000
VERSION     DAOTKS010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5361
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13720_length_5361_cov_3.962684"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="P5236_02310"
     CDS             <1..200
                     /locus_tag="P5236_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRU63226.1"
                     /translation="SQTIYRNILAYEEKNTLNGFLLLMHIGTDPKRSDKLYNRLDDLI
                     KELKHRGYELVRVDELLKNKK"
     gene            complement(227..1099)
                     /gene="lgt"
                     /locus_tag="P5236_02315"
     CDS             complement(227..1099)
                     /gene="lgt"
                     /locus_tag="P5236_02315"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRU63227.1"
                     /translation="MLNYVVWNVNPEIFRIGSLSVRWYGLMWAVGILLALFVGQRLFK
                     HEKLPEQWLDKLFFYAVIGGIVGARLGHCLFYEWELLPQPVKFLGITFKYGNYYLSHP
                     LEILYIWRGGLASHGGALGILVAMYFYNKNVSKKGFLWVFDRLVIGVALTGACIRIGN
                     LMNSEIYGTPTNLPWGFIFVRNGEVVPKHPTQIYEALYCLATFAVTWWLYWKKEAYKQ
                     TGLIFGVFLVMIFGIRFLLEFIKMDQEAFESGMILNMGQILSIPFIIWGIWLIVRRKK
                     TPAPALSTPKRKGS"
     gene            complement(1123..4419)
                     /gene="secA"
                     /locus_tag="P5236_02320"
     CDS             complement(1123..4419)
                     /gene="secA"
                     /locus_tag="P5236_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRU63228.1"
                     /translation="MALNNILKKILGNKSERDLNEIAPIIEKIQNVYPEIKALSNDDL
                     RARSQALKARINEYVAPEKNKIAELKASAETSEIDEQEKIYAEIDKLENEITDKYEKI
                     LNEILPEAFSIMKDTARRFVENEETEVTANDFDRNLAAKHDFVTIRGDKAYYKNEWMA
                     GGTLIKWDMIPYDVQLIGGVVLHQGKIAEMGTGEGKTLVATLPVFLNALTGNGVHIVT
                     VNDYLAKRDSEWMGPLYMFHGLSVDCIDKHAPNSEERRQAYLADITYGTNNEFGFDYL
                     RDNMAISPDDLVQRKHFYAIVDEVDSVLIDDARTPLIISGPVPKGDDQLFDELRPKVE
                     RLVNAQKNLVTKYLSDARMLLKSANEKEVEEGALALFRAYKGLPKNKPLIKFLSEPGI
                     KTTLLKTEEFYMQENNKNMPIATDPLYFVIDEKNNTIELTDKGIDLITDSSEDSDFFV
                     LPDVGSEIADLEKDTSLSLEEKQQRKDEILQNYAIKSERVHTIHQLLKAYTLFDKDVE
                     YVVMDNQVKIVDEQTGRILEGRRYSDGLHQAIEAKEHVTVEAATQTFATITLQNYFRM
                     YHKLAGMTGTAETEAAEFWDIYKLDVVVIPTNRPVIRDDMEDRIYKTKREKYAAVIEE
                     IVRLLDSGRPVLVGTTSVEISELLSRMLTGRKIKHNVLNAKLHQKEAEIVAEAGHRGT
                     VTIATNMAGRGTDIKLTPEVRDAGGLAIIGTERHESRRVDRQLRGRSGRQGDPGSSVF
                     YVSLEDDLMRLFGSERISKVMDRLGFQENEMIQHPMISRSIERAQKKVEENNFGIRKR
                     LLEYDDVMNSQREVIYTKRRHALMGERIESDIINMIYDEVDQIVETAKENANYGELQE
                     ELMTIFAIDVPFTEQEFNYSRTKDLVKIVAEAALNSFKRKMEKMAEIAYPVIKQVYEL
                     QGHKYENILIPISDGKKVYNITANLKTAYETQAKELVKSFEKQTLLYVIDNEWKEHLR
                     QLDELRNSVQNATYEQKDPLLVYKLESFGLFKEMIETINRKTLSILMRGQIPVREPEN
                     VKEAKIPAHQDYSKYKTQKEEFGNKGNGNKNDTREKQKPQPIHVEKKVGRNDPCPCGS
                     GKKYKYCHGA"
     gene            5023..>5361
                     /locus_tag="P5236_02325"
     CDS             5023..>5361
                     /locus_tag="P5236_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRU63229.1"
                     /translation="MMDKLFIFDTTLRDGEQVPGCQLNTVEKIQVAKALESLGVDIIE
                     AGFPVSSPGDFNSVVEISKTVTWPIICALTRAVEKDIEVAAEALKYAKRKRIHTGIGT
                     SDYHIKYKFNS"
BASE COUNT         1664 a          974 c          833 g         1890 t
ORIGIN      
        1 catctcaaac tatttatcgg aatattctcg cttatgaaga gaaaaacacg cttaatggtt
       61 ttttgctgct tatgcatata ggcacggatc cgaaacgttc cgacaagtta tacaaccggc
      121 tggatgatct gattaaagaa ttgaaacatc ggggatacga acttgtgaga gtggatgagt
      181 tgttgaaaaa taaaaagtga gtgaaatttt aattctccat taaattttaa gagccttttc
      241 gtttgggagt agaaagagcg ggtgcaggtg ttttttttct tcttacgatt aaccagattc
      301 cccagataat gaatgggata cttaaaattt gtcccatgtt caaaatcatt ccactttcaa
      361 atgcctcttg atccattttt ataaattcca ggaggaatcg gattccaaaa atcatgacta
      421 aaaacactcc aaaaatcaaa cctgtttgtt tataggcttc ttttttccag tagagccacc
      481 aagtcacagc aaaagtagca aggcaataaa gggcttcata aatttgcgta ggatgttttg
      541 gtaccacttc gccatttctt acaaaaataa atccccatgg aagattagtc ggagtaccat
      601 aaatttcaga attcattaaa tttccaatgc gaatgcaagc accggtaagg gcaacgccaa
      661 tcactaagcg gtcgaaaacc caaaggaaac ctttttttga aacattttta ttataaaaat
      721 acatggcaac taaaattccc aaagctcccc catgacttgc caatccacca cgccagatat
      781 ataaaatttc aagaggatga gataaataat aattgccata tttaaaagtt atgcctaaaa
      841 attttactgg ttgaggtaat aattcccatt catagaaaag acaatgtcct aaacgagcgc
      901 cgacaatgcc gccaatcaca gcataaaaaa acagcttgtc aagccattgt tcaggtaatt
      961 tttcatgttt gaaaagtcgt tgcccaacaa agagcgctaa caatattccc acagcccaca
     1021 ttaagccata ccatcgtacg gacaatgatc ctattcgaaa aatttcagga tttacattcc
     1081 agacaacata attcaatatt ggattcattc tttaattttc tatcaagctc cgtgacaata
     1141 tttatatttt ttcccgctgc cacaaggaca aggatcattt ctacccactt ttttttctac
     1201 gtgaataggt tgcggttttt gtttttcacg ggtatcattt ttattgccat ttcctttgtt
     1261 cccaaattcc tctttttgag ttttgtattt actgtaatct tgatgagcag gtattttagc
     1321 ttcttttaca ttttctggtt cacggacagg tatttgtcct ctcattaaaa tggataaggt
     1381 ttttctatta atggtttcta tcatttcctt aaaaaggcca aaggattcta acttatatac
     1441 caggagagga tctttttgct cgtaggtagc attttgaaca gaattacgca attcgtctaa
     1501 ttgtcgtaaa tgctctttcc attcgttgtc aattacgtat aataaagttt gtttttcgaa
     1561 tgattttacc aattcttttg cctgagtttc ataagcagtt tttaaattag ctgtaatatt
     1621 gtaaaccttt ttaccatcag aaataggaat aagtatattt tcatatttat gaccttgtaa
     1681 ttcgtaaact tgtttaataa caggataagc tatttcagcc attttttcca tttttcgctt
     1741 gaaactattt aaagctgctt cagcaacaat tttaacaaga tctttagtac gagaataatt
     1801 aaattcttgt tcagtaaagg gaacatctat ggcaaaaata gtcattaatt cttcttgcaa
     1861 ttctccataa ttggcgttct cttttgccgt ttctactatt tggtctactt catcataaat
     1921 catgttgata atatctgatt caatacgttc tcccattaac gcatgacgac gtttggtata
     1981 aataacttca cgttgtgaat tcatcacatc atcatattca agcagtcgtt tacgtattcc
     2041 aaaattattt tcttcaactt tcttttgagc tcgttcaatg gaacgtgaaa tcataggatg
     2101 ttgaatcatt tcattttctt gaaatccaag ccgatccatt acttttgaaa ttcgttctga
     2161 accgaataaa cgcattaaat catcttcaag cgaaacataa aatacagaag aacccggatc
     2221 gccttgacga ccagaccgac ctcgcaattg acggtctaca cgacgacttt cgtgacgttc
     2281 agtaccgata atggcaagtc caccagcatc tcttacttca ggcgttaatt ttatatctgt
     2341 accacgacct gccatattcg tagcgattgt aacagtgcct ctatgacctg cttccgctac
     2401 aatttctgct tctttttggt gcaatttggc attcaatacg ttatgtttaa tttttctacc
     2461 agtaagcatt ctgctgagta attctgaaat ttcaaccgaa gtagttccca ccaatacggg
     2521 tcgtccacta tcgagtaggc gaacgatttc ttctataact gccgcatatt tttcacgttt
     2581 agttttataa attctatctt ccatgtcatc acgaatcaca ggacgattag taggaataac
     2641 cactacatca agtttgtaaa tatcccagaa ttctgctgct tctgtttcag cggttcctgt
     2701 cattccagca agtttatgat acattcggaa ataattttgc aacgtaatgg ttgcaaaagt
     2761 ttgcgtggca gcttcaaccg taacatgttc ttttgcttca attgcttgat gcaatccatc
     2821 ggaatatctt cttccttcca gaatacggcc agtttgttca tctacaattt ttacttgatt
     2881 atccattacc acatattcaa catctttgtc gaataaagtg taggctttca ataattgatg
     2941 aatagtatga actcgttctg atttaatagc ataattttgc aaaatctcat cttttcgctg
     3001 ttgtttttct tctaatgata atgacgtatc tttttctaaa tcagcaattt cactacccac
     3061 atcaggtaaa acaaagaaat ccgagtcttc acttgagtct gttatcaggt caataccttt
     3121 atcggttaat tcaatggtat tatttttttc gtcaatgacg aaataaagag gatcggttgc
     3181 tataggcata ttcttgttat tttcctgcat atagaattct tctgttttga gtaaagtagt
     3241 tttgatacct ggttcactca aaaattttat taaaggttta ttttttggta gacctttata
     3301 tgctctgaat aatgcgagag ctccttcttc aacttccttt tcatttgctg attttaacaa
     3361 cattcgtgca tccgataagt atttggtgac caaattcttt tgagcattaa ctaatcgttc
     3421 tactttagga cgtagttcat caaacaattg atcatcgcct ttcggcactg gtccggaaat
     3481 aattaatggg gttcgtgcat catcaattag cacggaatcc acttcatcga caatagcata
     3541 aaaatgtttc ctttgtacca gatcatcggg actaatagcc atattatcac gaagataatc
     3601 aaaaccaaat tcattgttgg ttccgtaagt gatgtctgcc agataagctt gacgtcgttc
     3661 ttcagaatta ggagcatgct tatcgatgca atctacgctt aaaccgtgaa acatatataa
     3721 aggacccatc cattctgagt cacgtttagc aaggtagtca tttaccgtaa caatgtgaac
     3781 gccatttcct gtaagggcgt ttaaaaaaac agggagtgtt gcaaccaatg ttttaccttc
     3841 accagtaccc atttcagcaa ttttaccttg atgtaaaacc accccaccga ttaattgaac
     3901 atcataagga atcatatccc acttgataag tgttccacct gccatccatt catttttgta
     3961 ataagcttta tcgcctcgta tagttacaaa atcatgttta gctgccaaat ttctatcaaa
     4021 atcattagca gttacttcag tctcttcatt ttctacaaaa cgtcgagcag tatctttcat
     4081 aatgctgaaa gcttctggaa gaatttcatt taaaatcttt tcatatttgt cggtgatttc
     4141 attttccagt ttgtcaattt cggcgtagat tttttcttgt tcgtcaattt cactggtttc
     4201 tgctgatgct tttaattcag cgattttgtt tttttcagga gctacatatt catttatcct
     4261 agctttcaaa gcttgcgaac gagctcttaa atcatcattg gaaagagctt taatttcagg
     4321 ataaacgttt tggatttttt ctataattgg ggcaatttcg tttaaatctc gttcagactt
     4381 atttcctaaa atttttttta aaatattatt taatgccatt tatatcaatt ttttttagat
     4441 tcgattttat tattatgcaa acctttataa aacaatttta ttatcaaaaa gatttacaaa
     4501 aataacactt tttatgtcga tagcctatta tttatatcaa aaattgttat aaagctttca
     4561 aaaataaaaa gttatcaaca cattttcaaa aataattttt ctaagtattt tgtttttctc
     4621 attttaactg tgaataaaaa aaccttttat atgaattata taaacaattg aaaaccaaat
     4681 ttttaattgt ttgaaaagta ttaaagtagc attttattat ggaaaaaatt aaaaatcata
     4741 ataaaatcca ttgaaaaaat tttattttat atttgactta ttcaaaaatt gatttatctt
     4801 tgcagtgtaa ataataatat tgcaatgaat aaacattcct tacatatact tctactgggc
     4861 ctcattattc taattttaca gaataagtga agtgagtttg gtattgtagg gatagtataa
     4921 aaatgtctaa aagcctttct cactcgctta ggagtgagaa aggctttttt tatgggaata
     4981 agtggaaaaa ttataggagt ttttatattt aaaaaattat aaatcatgga taaattattt
     5041 atttttgaca ctactttacg tgatggcgag caagttcctg gttgtcaact gaatacagtt
     5101 gaaaaaattc aggttgcaaa agctttagaa agcttagggg tcgatataat tgaagctggc
     5161 tttcctgttt caagtccagg tgattttaat tcggtagtgg aaatttcaaa aactgttacc
     5221 tggccaataa tttgtgcttt aacacgtgct gttgaaaaag atattgaagt ggctgcagaa
     5281 gccttaaaat atgccaaaag aaaacgaatt catactggaa ttggaacgtc agattatcat
     5341 atcaaatata aatttaattc t
//
LOCUS       DAOTKS010000072         5326 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_13812_length_5326_cov_3.944413, whole genome shotgun sequence.
ACCESSION   DAOTKS010000072 DAOTKS010000000
VERSION     DAOTKS010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13812_length_5326_cov_3.944413"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="P5236_02330"
     CDS             complement(<1..488)
                     /locus_tag="P5236_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRU63230.1"
                     /translation="MVNDPFQNRRHIISIIISVFIGIFIIRLFSLQVIETKYKKGADS
                     NAFLNKTLYPPRGLIYDRNHNLLVYNKPAYDLMLIVREIHDLDTTAFCEALEINKQFF
                     IQRMAEIKDRKKNRGYSSYTPQVFMTQLDIKDIANIQQQLYKFPGFYIQNRTLRDYRY
                     PNA"
     gene            complement(491..988)
                     /gene="mreD"
                     /locus_tag="P5236_02335"
     CDS             complement(491..988)
                     /gene="mreD"
                     /locus_tag="P5236_02335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HRU63231.1"
                     /translation="MTLALQNIIRFVLLVLAQVLILNNIQFLGYINPYIYILFIFSLP
                     YRTPQWLILSFAFILGLTIDIFSNTLGMHSFATVLMAFTRNGVIQLFTSFEKEENPIP
                     SFRTFGISAYLKFIIVMVLIHHTTLFLLEAFSFSNFFFIFKKILLSSMISIVLILGIQ
                     SFKKK"
     gene            complement(989..1828)
                     /gene="mreC"
                     /locus_tag="P5236_02340"
     CDS             complement(989..1828)
                     /gene="mreC"
                     /locus_tag="P5236_02340"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRU63232.1"
                     /translation="MKNLINFLIHHSGLLLFIILEIISFIMMINSQEYQKSIFLSSSN
                     AVVATLYNWTNSVGEFFKLRNTNNQLSSENTELKNQIIELQNKLKVLEPSITSLNFKL
                     LPENEYRFISAKVINNSTDKFQNYLTLNKGSKDGIKPDMGVVSSEGVVGIIKSVSPHF
                     SVVIPILNPKIQISCKFKRTDYTGSLVWNGEDYRYANLVDIARHVDIRLGDTIVTSGF
                     TRTFPEGIPVGIVEDYNIKESDAYYNIKVKLAVNFRTLTYVNVINYLNYQEQKQLEEK
                     TYE"
     gene            complement(1928..2947)
                     /locus_tag="P5236_02345"
     CDS             complement(1928..2947)
                     /locus_tag="P5236_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRU63233.1"
                     /translation="MGLFSFTQEIAIDLGTANSVITYNDQIVVDEPSVVALDKRTDRL
                     IAVGERARQMQGKENEAIRTIRPLRDGVIADFYAAEMMIRGLIKMIPTKNHFFSPSLR
                     MVVGIPSGSTEVEIRAVRDSSEHAGGREVYMIYEPMAAALGIGIDVEAPNGCMIVDIG
                     GGTTEIAVISLGGIVLNKSIRIAGDDLTMDIIEYMGRMHNIKIGERTAELIKINVGAA
                     LTDLEDGPEDYIVRGPNRMTALPIEVPVSYQEIAHCLEKTISKIEAAVLSALEETPPE
                     LYSDIVKQGIYLCGGGALLRGMDKRLTEKINIPFQIADDPLHAVARGTGVALKNIDKF
                     SFLLR"
     gene            complement(2999..4519)
                     /gene="purH"
                     /locus_tag="P5236_02350"
     CDS             complement(2999..4519)
                     /gene="purH"
                     /locus_tag="P5236_02350"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRU63234.1"
                     /translation="MEEKRIKKALISVYHKDHLEDIIIKLHSEGVALISTGGTKKFIE
                     SLNLPCQSVEELTGYPSILGGRVKTLHPKIFGGILARRDIEEDLEQLKQYEIPEIDLV
                     IVDLYPFEATVNAGADEPTVIEKIDIGGISLIRASAKNFNDVVIIASQAQYEPFMEIL
                     TTKGAQTTLEERRWFAKEAFAVTSQYEIAIFNYFDRSYQSAFRYGENHPKILRYGENP
                     HQQGIFYGKFDEMFEQLQGKEISYNNLLDIDAAVNLINDFDDITFAILKHNNACGIAT
                     RPQLKDAWQAALAGDPVSAFGGILITNAIVDKETALEINKIFFEVLIAPDYDLEAIEI
                     LSQKKNRIILIMKSTKLKPKQFRSILNGVLTQDRDTHIETINDLKIVTEKSPTAEEIE
                     DLLFANKVVKHSKSNAIVLAKNKQLLGSGVGQTSRVDALKQAIDKAKSFNFDLKGAVM
                     ASDAYFPFPDCVQLAYEAGITAIIQPGGSIRDKDSIDLCNQNRISMVFTGYRHFKH"
BASE COUNT         1762 a          892 c          816 g         1856 t
ORIGIN      
        1 gcattaggat acctgtaatc tcttaatgtt ctgttttgaa tgtaaaaacc gggaaattta
       61 tacaattgtt gctggatatt ggctatgtct tttatgtcca gttgtgtcat aaatacctga
      121 ggagtatagg aagaataccc tcgatttttc ttcctatctt ttatttctgc cattcgttgg
      181 atgaaaaatt gtttgttaat ttccaaagct tcacaaaagg cagtagtatc taaatcatga
      241 atttctcgta caataagcat taaatcgtaa gcaggtttat tgtaaaccag caaattatgg
      301 ttccgatcat aaattagtcc tcgtggtgga tacagggttt tatttaaaaa agcgttgcta
      361 tctgctcctt ttttatattt tgtttcgatt acctgcaaag aaaacagacg gataataaat
      421 ataccaatga atactgatat aataatggaa attatatgcc ttctgttttg aaaaggatcg
      481 tttaccattt ctattttttc ttaaacgatt gaatgcctaa gattaataca atacttatca
      541 ttgagctaag gagaattttt ttaaagataa agaaaaaatt cgagaaggaa aatgcttcta
      601 acaaaaacaa agtagtgtga tgaatcaata ccattacaat aatgaatttt aagtaggcac
      661 tgatgccaaa agttctgaag gatggaatag gattttcttc tttttcaaag gaagtgaaca
      721 gttgaataac accatttctt gtaaaagcca ttaaaaccgt tgcaaaagag tgcatcccca
      781 aagtatttga aaaaatatca attgttaatc ccaaaataaa agcaaaagaa agaattaacc
      841 attgaggagt acgataaggc agagaaaaaa taaaaagaat ataaatataa ggattaatgt
      901 atcctaaaaa ctgaatatta tttaaaatca atacttgagc caataccaat aaaacaaaac
      961 gaataatatt ttgaagagct aacgtcattt attcataagt tttttcttcc agttgctttt
     1021 gttcttgata atttaagtag ttaatgacat ttacataagt aagggttcga aaattaacag
     1081 caagttttac tttaatgtta taataagcat cgctctcctt aatattataa tcttctacaa
     1141 ttccaacagg aattccttca gggaaagttc tcgtaaaacc actggttaca atcgtatctc
     1201 ccaatctaat atctacatgt cttgctatat ctactaaatt ggcataacga taatcctctc
     1261 cattccaaac cagagaacca gtataatcag ttcgtttaaa tttgcaactg atttgaattt
     1321 ttggatttaa aattggaata actacagaaa aatgaggaga taccgactta atgattccga
     1381 ctactccttc gctgctaaca acacccatat cgggtttaat tccatctttt gatcccttat
     1441 taagagtgag atagttctgg aatttatctg tactgttatt gattacctta gcagagatga
     1501 atcgatattc gttttcaggt aataatttaa aatttaaaga ggtaatggaa ggttctaaga
     1561 cttttaattt attctgtaat tcaataattt gatttttaag ttcagtattt tctgatgata
     1621 gttgattgtt cgtatttcgt aatttaaaaa attctcctac tgaattggtc caattgtaaa
     1681 gagtagcaac tactgcatta ctcgaagaaa gaaaaatact tttttgatat tcttgtgaat
     1741 taatcatcat aataaaagaa ataatttcaa gtattataaa taataacaaa ccactatgat
     1801 gtatcaaaaa atttatgagg tttttcataa taaatacaat tctgcaggga ttgtttttaa
     1861 aataaaaatt cagccaaatg aaaatgaagc attcatcatt ttaatttatt ttgttgaatt
     1921 tttaaattta tcgaagtaaa aaagaaaatt tatcgatatt ttttaaggca acacctgttc
     1981 ctcgagccac cgcatgtaaa ggatcatcag caatttgaaa aggtatattg attttttctg
     2041 ttagacgttt gtccatcccc cgaagtaatg ctccacctcc acataaataa attccttgtt
     2101 ttacgatatc tgaatatagt tcaggtgggg tttcttccag agcacttaac actgctgcct
     2161 caattttgga aatcgttttt tcaagacaat gagctatttc ttgataagaa actggaactt
     2221 caataggcaa ggcagtcatt cgattgggac cacgaacaat gtaatcttct ggtccatctt
     2281 ctaaatcggt taatgctgca ccaacattta ttttgattaa ctctgctgtc cgttcgccaa
     2341 ttttaatatt gtgcattcgt cccatatatt caatgatatc catagtgaga tcgtcaccag
     2401 cgatacgaat agatttattc aacacaatgc cacccaacga aataactgca atttctgtag
     2461 taccaccacc aatatctaca atcatacagc cattaggggc ttctacatca atacctattc
     2521 ccaaagcagc agccattggt tcataaatca tataaacttc cctgcctccc gcatgttctg
     2581 acgaatcacg tacagcacga atttccactt cagtacttcc agagggaata cctactacca
     2641 ttcgcaaaga aggcgaaaag aaatgatttt tagttggaat cattttgata agtcctctta
     2701 tcatcatttc tgctgcataa aaatcagcaa ttacaccgtc acgtaatggt ctaatagtac
     2761 gaatagcttc attttctttc ccctgcatct ggcgggcgcg ttcacctact gctattaatc
     2821 ggtctgtacg cttatccaga gcaacaaccg aaggctcatc aacaactatc tggtcattat
     2881 acgtaataac actgtttgca gtacctaaat cgattgctat ttcttgtgtg aatgaaaaaa
     2941 gtcccatttt ttataaatga tatttgtatg ttaatgatat attgtatttt taaaaaaatt
     3001 aatgtttgaa atgtcggtag ccggtaaata ccattgagat tctattctgg ttgcataaat
     3061 ctattgaatc tttatctctg atagaacctc caggttgaat gatggctgta atccctgctt
     3121 cataagcaag ttgtacgcag tcagggaaag gaaaataagc atccgacgcc attacagctc
     3181 cttttagatc aaaattaaat gatttagctt tgtcaatagc ttgtttcagt gcatcgactc
     3241 ttgaagtttg tccaacgccg cttcctaaca gttgtttatt ttttgctaga acaattgcat
     3301 ttgattttga atgttttaca actttatttg caaaaagtaa atcttcaatt tcttcggcag
     3361 taggtgattt ctcagttact atttttaaat catttatagt ttctatatga gtatctctgt
     3421 cttgagtaag cactccattg agtatagaac gaaattgttt gggttttaat ttcgtacttt
     3481 tcataatcag aatgattcga ttttttttct ggctcaaaat ttcaatggct tctaaatcat
     3541 aatcaggggc tatcaacact tcgaaaaaaa tcttattaat ttcaagagca gtttctttat
     3601 caacaatggc atttgtaatt aatatccctc caaatgctga gacaggatct cctgctaatg
     3661 ctgcttgcca agcgtctttt agttgaggac gtgtagcaat tccgcaagca ttgttgtgtt
     3721 ttaaaatagc aaaagtaata tcgtcaaaat cattaattaa atttacggcc gcatcaatgt
     3781 ccaagagatt gttataagaa atttctttcc cttgcaattg ttcaaacatt tcatcgaatt
     3841 ttccgtagaa aattccttgt tgatgaggat tttcaccata tctgagtatt ttgggatgat
     3901 tctcgccata tcgaaaagcg ctttgataag aacggtcaaa atagttaaaa attgcaattt
     3961 cgtattgtga agtcacagca aaagcttctt tagcaaacca gcgacgttct tcaagcgtag
     4021 tttgagctcc tttggttgtg agaatttcca taaaaggttc gtattgagct tgggatgcta
     4081 ttattaccac atcattaaaa ttttttgctg atgctcgaat aagtgaaata ccacctatgt
     4141 cgattttttc aataacagta ggttcatctg ctccagcatt taccgtagcc tcaaaaggat
     4201 ataaatcaac tataaccaaa tcaatctcag gtatttcata ttgctttaat tgttccagat
     4261 cttcttcaat atcccttctg gcaagtatcc caccaaatat ctttggatga agagttttta
     4321 ctcttcctcc taaaatggaa ggataaccag ttagttcttc cactgactga cagggtaggt
     4381 ttaaagattc aatgaacttt tttgtgcctc ctgtagaaat aagagcaact ccttcggaat
     4441 gtaattttat gataatatct tctaaatggt ctttgtgata gactgatatt aaggcttttt
     4501 taattcgttt ttcttccatt ttttttgtat ttaatttatt gacaaagtta taaaaaaata
     4561 gtggttttat attaactaaa tagggataga taaaacaaca aattattttt aataaatcta
     4621 cacaaaaata aacaaaactc aaattcaatg aattgagaat ctataggttg gattgcaaat
     4681 agaatgcacc tgaattttga ctataagtca attaaaatgt taaactaaag tttattgaaa
     4741 ttattcaaga ataaaattct catctggatt gggttgcctc ttgtgatgct ctatataaga
     4801 atttatcatc ttttctgtta cattgcctgc gctccaacaa ccatagccta tttacccaaa
     4861 aatgtgccac caataacgat gttgtagttt aggaaatttt tgcaatacca tccggcaact
     4921 ttttcctttc aatcgcttga ctattttaat tacacttaat tgggagataa taaaatggat
     4981 atgatctttg ctaactactc cttgtaaaaa atctctatat ccttgtcgat tctttatctc
     5041 attttttata gcaaatttaa aaaagttttc tataaactaa attcttgtat gtaaagtgta
     5101 tagttttaat ttaacgtgtt tgaaaaataa gaaaataaca attcccatta actattctta
     5161 tatttttatt cttttgtatg tttgttaaaa aaatataaga taaatttgaa agcaagttgg
     5221 tatcaaatct cggtattcaa gagattcatt tttgctccac cgctaaaact cgctaagttc
     5281 aacatagaag tgcgaattta gcagagatgt tttgcatgat ttttta
//
LOCUS       DAOTKS010000073         5284 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_13932_length_5284_cov_4.995984, whole genome shotgun sequence.
ACCESSION   DAOTKS010000073 DAOTKS010000000
VERSION     DAOTKS010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13932_length_5284_cov_4.995984"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="P5236_02355"
     CDS             <1..312
                     /locus_tag="P5236_02355"
                     /inference="COORDINATES: protein motif:HMM:NF015247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HRU63235.1"
                     /translation="EVAVVNYPNDYDFTRITEFKHIHPVETGKTVILKEYPQDYQMGK
                     NLPFYPILKEKNLELYHRYLELAKKFKNLILVGRLADYQYYDMDDIIKKSLEIFEEKV
                     K"
     gene            309..1370
                     /locus_tag="P5236_02360"
     CDS             309..1370
                     /locus_tag="P5236_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63236.1"
                     /translation="MSIQIQEDSIIYVVAPANDVSGGPELLHQLVFHLRKDLKINSYL
                     YYVPVNEISPTPFEYEQYNVPFINEIEDEEKNLLIVPEIKEYVDILKKYVNIRKCIWW
                     LSIDRFYLSLVISDKKKFFFQRLINKISRIFFGKQFIDIETEIYRKINFKVLECPEWI
                     KNIDFHFVQSCYAFHHLLNKGIPKNKIFYLSDYLNKNFLKIHTNLSQKQNIVAYNPKK
                     GYAFTQQLVKQAKNITFIPIINMDRQQVIMTLQKAKIYIDFGNHPGKDRLPREAAILG
                     CCVITDKKGSAAFYEDVSIPDKYKIEDKEENIPEIIEKINECFENFEEKYKDFKSYRE
                     IILREPQKFLEDLKNIFIN"
     gene            1650..2957
                     /locus_tag="P5236_02365"
     CDS             1650..2957
                     /locus_tag="P5236_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRU63237.1"
                     /translation="MITSEDFLQKIESNQIIVGIIGLGYVGLPLATTFAQASIKVIGF
                     DKNPDKVKKINSGENYISDISDEEFCEIISDSEFTATDDFSRIKECDALIICVPTPLD
                     IFQKPDMSFIESACIEIGQNMKRGTFISLESTTFPTTTENFMLPIIERESGLKHGVDF
                     WLAYSPERVDPGNQTYKTKNTPKVLGAISEEGLKIGEALYKKAIQNVHLVSSPRVAEM
                     VKILENTYRLINISLINELALLAGKMDINIWEVIEAAKTKPFGFQAFYPGPGIGGHCI
                     PLDPFYLEYIAKKFDFDLTMIHTAGHINMRMPHHMYNKITSALNRQKKAINGSKIFFL
                     GVAYKPNISDERESPALEIIDIIANKGGIVSYNDPYIPSITTPEGYSFQSTDYSAENL
                     ENADLVVITTNHSIYKADFIMQHAHLIVDLRNVIPPANNVYKL"
     gene            2960..3922
                     /locus_tag="P5236_02370"
     CDS             2960..3922
                     /locus_tag="P5236_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRU63238.1"
                     /translation="MTKKICVVGGGKWGQNHIRTLYQLGNLGGIVEANSEYLSELLKL
                     YPVEGFIELEQALEKDFDGFTVAVPAPLHYEVTKKIIESGKSVLVEKPMTLSTETSRE
                     LVELAERKGVQLMVGHVLLFHPAIRKIKKIIDSGELGNLYYLYSNRLNLGTVRTEENV
                     FWSFAPHDISVLDYFVGHSAEKIVSKGSKFLQENVYDVTITQLDYPNNVHAHIFVSWL
                     HPFKEQRLVVIGSKGMISFDDASIDKEIKFYDKRIHFEKGIPVKVENPTRIIPYEQKQ
                     PLTEELKYFIEHLDKKIEIADGRSGLEVVKVLEEAEKKLRIGSQ"
     gene            complement(4313..>5284)
                     /gene="ffh"
                     /locus_tag="P5236_02375"
     CDS             complement(4313..>5284)
                     /gene="ffh"
                     /locus_tag="P5236_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRU63239.1"
                     /translation="AKLANFLKTKRGKNPLLVACDVYRPAAIEQLKVLGEQIQVPVYA
                     ELENKNPISIAESAVKYAKLHGYDVLIIDTAGRLAIDEMMMTEISAIKQAIQPQEILF
                     VVDSMTGQDAVNTAKEFNDRLDFDGVVLTKLDGDTRGGAALSIRSVVNKPIKFVSTGE
                     KMDALDVFHPERMADRILGMGDIVSLVERAQEQYNEEEARRLQKKIAKNQFDFNDFLS
                     QIHQIKKMGNFKDLASMIPGVGKALKDVDIDENAFKSIEAIIQSMTPEERENPSLLNG
                     SRKQRIARGSGTTIVEVNRLIKQFDQISKIMRVATVGSNKMKMKKKK"
BASE COUNT         1821 a          836 c          893 g         1734 t
ORIGIN      
        1 gaagttgctg tggtaaatta tcccaatgat tatgatttta caagaatcac cgaatttaag
       61 catattcatc ctgttgaaac aggtaaaaca gttattctga aagaatatcc acaagattat
      121 cagatgggta aaaatttacc tttttatcca atattgaaag aaaaaaattt agaactttat
      181 catcgctatt tagaactggc aaagaaattt aaaaatttaa tattggttgg acgactggct
      241 gattatcagt attatgatat ggatgatata attaaaaaat cactggaaat ttttgaagag
      301 aaagtaaaat gagtattcaa atacaagaag atagcattat ttatgttgtg gcacctgcta
      361 atgacgtttc aggaggtcca gaattattgc atcaacttgt ttttcatctc agaaaggatt
      421 taaaaatcaa ttcttatctt tattatgttc ctgtaaatga aatttcccca actccttttg
      481 agtatgaaca atataatgtt ccttttatta atgaaattga ggatgaggaa aaaaatttat
      541 taattgttcc tgaaataaaa gaatatgtgg atatattaaa aaaatatgtc aatataagaa
      601 aatgtatttg gtggttgagc attgacagat tttacttatc tttagtgata agtgacaaga
      661 aaaaattctt ttttcaaaga ttaattaata aaatttctcg aatatttttt ggtaaacaat
      721 ttatagatat tgagacagaa atttatagaa aaatcaattt taaagtttta gaatgtcctg
      781 aatggataaa aaatattgat ttccattttg ttcaaagttg ttatgccttc catcatttgc
      841 tcaataaagg aataccaaaa aataaaatat tttatctttc tgattatttg aataagaatt
      901 ttcttaaaat tcacacaaat ttatcacaaa aacaaaatat tgtagcctat aaccctaaaa
      961 aaggatatgc atttacccaa caacttgtta aacaagcaaa aaatataact tttattccca
     1021 tcataaatat ggatcgccaa caggtgatca tgacccttca aaaagctaaa atatatattg
     1081 attttggcaa tcatcccggt aaagatcggt taccaagaga agctgctatt ttaggttgtt
     1141 gtgttattac tgataaaaag ggttcagccg cattttacga agatgtttca atacctgata
     1201 aatataaaat agaagacaaa gaggagaata tccctgaaat aatcgaaaaa atcaatgagt
     1261 gttttgaaaa ttttgaagaa aaatataaag attttaaaag ttatcgagaa ataatcttac
     1321 gagaacctca aaaatttctg gaagatttaa aaaatatttt tataaattaa gtctaaaaca
     1381 atcaacaagc aatttttcaa tttctgtgat ataatgataa aacaaaatca acaaattttt
     1441 tcagctttga aaaaaattga aaattattaa ctaaatttgg gtcttgaaat aacaggctta
     1501 ttttaaacaa actactgtgt tattcaatca attatgtttg ctgtttaatt tttttctata
     1561 taagaaacct aataaaaatt agacttgttt ttaaaaccta tttaaatcca ttatcaaaaa
     1621 aatttagata tttaattcgt aaataaaaga tgattacttc tgaggatttt ttgcagaaaa
     1681 ttgaatccaa tcaaattatt gtaggaataa ttgggttggg ttatgttggt ctgccattag
     1741 caactacttt tgcacaagct tctataaaag ttattggatt tgataaaaat cccgacaaag
     1801 taaaaaaaat aaatagcgga gaaaattata tttccgatat ttctgacgaa gaattttgcg
     1861 agattatttc cgatagtgaa ttcaccgcca ctgacgactt ttctcgcatc aaggaatgtg
     1921 atgcactgat tatttgtgtg ccaacgccgc tggatatctt tcaaaaaccc gatatgagtt
     1981 ttatcgaaag tgcttgcatc gaaatcgggc aaaacatgaa acggggtact tttatcagtc
     2041 tggaaagtac tactttccct actacaacag aaaattttat gttgccgatt attgaacgcg
     2101 aatctggact aaaacatgga gttgatttct ggctggctta ttcacctgaa cgagttgatc
     2161 cgggtaacca aacttacaaa actaaaaata caccaaaagt attaggagct atcagcgaag
     2221 agggattgaa aattggcgaa gcactttata aaaaagctat tcaaaatgtg catttggtta
     2281 gttctccacg cgtagccgaa atggtcaaaa ttcttgaaaa tacttatcgg ctgattaata
     2341 tcagtttaat caatgaattg gcattgctgg ccgggaaaat ggatattaac atctgggaag
     2401 tgattgaagc tgctaaaaca aaaccttttg gctttcaagc tttttatcca ggtcctggca
     2461 ttggcggaca ttgcataccg ctcgatcctt tttatctgga atatatcgct aaaaagtttg
     2521 attttgattt aaccatgatt catactgctg gacatatcaa tatgcgtatg ccacaccaca
     2581 tgtataataa aattacatca gctttgaata gacagaaaaa agctatcaac ggaagcaaaa
     2641 tattcttttt aggtgttgca tataagccta atattagtga tgaaagagaa tctcctgcac
     2701 tggaaattat tgatattata gcaaataaag gaggaatcgt ttcttataat gatccttata
     2761 ttccttccat taccactccc gaaggttatt cttttcaatc aactgattac tctgctgaaa
     2821 atctggaaaa tgcagactta gtagtgatta caaccaatca ttctatttat aaagcagact
     2881 ttattatgca acatgctcat ttgattgtgg atttaagaaa tgtcattcca ccagctaata
     2941 acgtttataa actttaaaaa tgacaaaaaa aatttgtgtt gttggcggtg gcaaatgggg
     3001 acaaaaccat attagaacgc tttatcaact gggaaatttg ggcggtattg ttgaagccaa
     3061 ttccgaatat ttgagcgaat tgctgaaact ttatcccgtt gaaggattta tagaattgga
     3121 acaggcattg gaaaaggatt ttgatggatt taccgttgct gttcctgctc cactgcatta
     3181 cgaagtgacc aaaaagatta ttgaaagtgg aaaatcggtt ttggttgaaa aacccatgac
     3241 tttatccact gaaacttcca gagaattggt tgaactggct gaaaggaaag gcgttcaatt
     3301 gatggtggga catgtgttgc tttttcatcc ggctattcgt aaaataaaga aaataattga
     3361 cagtggagaa ttgggaaatc tttattatct ctattccaat agattaaatt tgggaacggt
     3421 tcgtacggaa gaaaacgttt tctggtcgtt tgctccgcac gatatttccg ttttggatta
     3481 tttcgtgggt cattcggctg aaaaaattgt ttcaaaaggt tcaaaatttc ttcaggaaaa
     3541 tgtgtatgat gtaaccatca ctcaattgga ttacccgaat aatgttcatg cccatatttt
     3601 tgtttcgtgg cttcacccgt ttaaggagca acggttggtg gtcattggta gcaagggaat
     3661 gatttcgttt gatgatgctt ccattgataa ggagattaaa ttttacgata aacgcattca
     3721 ctttgaaaag ggtattcccg tgaaagttga aaatcctacc agaatcattc cttatgaaca
     3781 aaaacaacca ctcaccgaag aattgaaata ttttatcgaa catctggata aaaaaatcga
     3841 aatagccgat ggacgatcgg gtttggaagt ggtaaaagtg ctggaagaag cagaaaaaaa
     3901 gttaagaatt ggtagtcagt agcttgtaat taaaaaatat tggtacgtaa attcaagtaa
     3961 tttaatcgta agaattcccc gatgcttgcg tcggggtagc ttttgttttt atttttttag
     4021 aaattccggt ggcgaagcca taaaattggc gtaatatctc gcttcttgcg gcgaggatag
     4081 tcaattttaa ggaatttctt taatatgcca tttttagaga aaattataac tacactgaaa
     4141 gcgggaaaag aatgtttata aaattatggg aaacatctaa agaataagag ataaacaaat
     4201 acaaaaattc tgtgaagaaa ttttaacaac ctatcaattt ttacccatta taaaaaaatc
     4261 tcaatctttt ttgccgacaa catttctaat agaaaaaaac gttaacattc ttttatttct
     4321 tcttcttcat tttcatttta ttagaaccta ctgttgccac tcgcattatt ttgctaattt
     4381 ggtcaaactg tttgataagc ctatttactt ccacgatggt tgttccactg cctcgagcta
     4441 ttctttgttt acgacttcca ttcagtaagg aaggattttc acgttcttcg ggcgtcatag
     4501 attgaataat agcttcaatc gacttgaaag cgttttcatc aatatctaca tctttgagag
     4561 ctttccccac tcctggaatc atggaagcca gatccttgaa attgcccatt tttttgattt
     4621 gatgaatttg tgacaaaaaa tcgttaaaat caaattgatt ttttgcaatt ttcttctgca
     4681 gtcgtcgtgc ttcttcttca ttatattgtt cctgagctct ttcaaccaac gaaacaatat
     4741 cgcccattcc taaaattcta tcagccattc gttcaggatg gaaaacgtcc agtgcatcca
     4801 ttttttcacc ggtacttaca aattttatag gcttgttaac aacagaacgt atggaaagag
     4861 cagcaccacc acgcgtatca ccatcaagtt tagtcagcac gacgccatca aaatcaagtc
     4921 gatcattgaa ctcctttgct gtgttcactg cgtcctgacc tgtcatagaa tcaactacaa
     4981 acaaaatttc ttgtggctga atagcttgct taatggcaga aatttcagtc atcatcattt
     5041 cgtcaattgc cagacgacca gcagtatcta taatcagcac atcgtaaccg tgaagctttg
     5101 catatttaac tgctgattct gcaatagaaa taggattttt gttttctaat tcagcataaa
     5161 ccggcacttg tatttgttct cctaacactt tcaactgttc aatagcagca ggacgataca
     5221 catcgcaagc taccaataaa ggattttttc ctcgttttgt ttttaaaaaa ttggctaatt
     5281 tagc
//
LOCUS       DAOTKS010000074         5274 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_13960_length_5274_cov_3.970301, whole genome shotgun sequence.
ACCESSION   DAOTKS010000074 DAOTKS010000000
VERSION     DAOTKS010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13960_length_5274_cov_3.970301"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(676..1701)
                     /locus_tag="P5236_02380"
     CDS             complement(676..1701)
                     /locus_tag="P5236_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HRU63240.1"
                     /translation="MKKVFVIEILSIFFLTGFTQPQEKQNFKVVFYNTENFFDCFDDS
                     LTNDAEFLPDGTRGWTFAKYKKKQNNIGKVIASVGGWNAPALVGLAEVENERCLIDLT
                     HYSGLKNLHYKFLHFESPDPRGIDVALLYQPQFFKPLISKPIPIHFSTSPQIKTRDIL
                     YAAGIIPTDDTLHVFVCHFPSRLGGELESENKRVEVASILRTTIDSIYQVQNHPYIVI
                     MGDFNDYPTNKSLLKTLRALPPSDSIYSENLYNLMYPLHIKGKGSHKYNGEWGALDQF
                     IVSGALLKAGGKFYTLPMDAHFFEADFLLEDDKTNLGKQPFRTYVGMKYQGGFSDHLP
                     IYLDFWY"
     gene            complement(1711..3123)
                     /locus_tag="P5236_02385"
     CDS             complement(1711..3123)
                     /locus_tag="P5236_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63241.1"
                     /translation="MLNFLSCEKDNFTTNPLHTIEFSTDTITFDTLFTTIGSTTAKVM
                     VYNHNSKAVRISTIALAQGGNSFFRINVDGAVNENNFFTNVEIPAHDSIYIFVEVTID
                     ATDSDVPVLMEDKIVFLVNGIVKQIQLQAYGQEVEIFRDKIIWNDTLLTAKKPYLIYG
                     NLIIDTARTLTLEAGTKFYFHHNANLIVYGNLRAEGTFDHPILMRGDRFDKINFETPI
                     PYNVVAGQWGGIYLLGENGEHLLRHVKMNSGYVGIYLSNQNINSLPHLEINSCYIHNF
                     LRYGLVIQNANVDVINTEISNTSSYCVYLNGGTHSFIHCTIVNYFNNSNVQPVSRGSD
                     PAFMIMTLNRVAPMHTAIKNSVITGTLENEFSLVTRFLDEYKGTFSNNYIRKYEPLDL
                     PQFTNIRWYEKNDTVFKNIYYDYKKNNYFNFEPDSISPIRDLGDYTIATEYCPLDLNG
                     NNRLADGKPDAGAYEWKPTE"
     gene            3296..3601
                     /locus_tag="P5236_02390"
     CDS             3296..3601
                     /locus_tag="P5236_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63242.1"
                     /translation="MASRRKLKKRINEISSNLLGEIYLRYLLFDIQDEEKFNQLLSNI
                     LLMRKEFISKINHIDGKNNPVMVKKYFRQFFSDWGTKITEIIEEIEKLSPVKQESEK"
     gene            complement(3684..4337)
                     /locus_tag="P5236_02395"
     CDS             complement(3684..4337)
                     /locus_tag="P5236_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRU63243.1"
                     /translation="MIIFAQGNLPYVDDKPYHFGFLVGLNTADFGIVHKDTLIDNKAY
                     HVSVSTLQPGFSVGIITNLRLSKYWNLRFTPTLHFSERTLTYRADTSSITTSTNINSI
                     PLCLPLYIKYSAERKDNYRPYLLGGGGIYYDFGRNKEKAILLKPTDYYVEFGVGCDLY
                     FSFFKLAPELKFALGFNNILTPLKERDAGGLTEQDKLHNNVLSKLTSKMLTLCFNFE"
     gene            complement(4733..5071)
                     /locus_tag="P5236_02400"
     CDS             complement(4733..5071)
                     /locus_tag="P5236_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63244.1"
                     /translation="MAQQIYNAAGKLVAKVDENCFYNSGGKLMGKVEGVFVYDNAGKL
                     MGKVDGVYIYDEKGKLIGKVDGNNLYDALGRKMATIIDKDLYDSYGRKIRRFNGLTQS
                     QIITYYYYFY"
BASE COUNT         1943 a          824 c          765 g         1742 t
ORIGIN      
        1 agttcataaa cgtacggcag agcatttgaa aagtttctac tgcttagttt tatatcattg
       61 aagtaaaaaa gtaaaaagag actactacgg atatcatact aaccaaaaag aaaagttccg
      121 ctgaaaatga acagtttcag ggaatttatt ttatcaaact cccttaaaag cagaattaga
      181 agaaatcgta tgggtgatag aaaatacttt tagatgtttg aaaactgatt tggatttacg
      241 ttctattcat cacaaaaagg attcattatc aataacagga aattatccag attaccaata
      301 cgcaaaaaag ttgctattac ctataaaaaa catttttaac gaaactattt acattcggtg
      361 gtacactaaa cctaataaaa aggtaaaaac tacttatcag gcatcagaat acagatatta
      421 cccatttgtc aaatgaaaat cgggaggaca taaatctgaa ttcaaaaaaa tcaattctct
      481 cccttctgca aataaacgat agttaactgc aatgtgaact aaaagcaagt tgatcacaac
      541 aagtctgagc taaattataa tattaatagt aacccccctt ttgaactatt ttactaacgt
      601 tcaatccagt ttgtgggtaa aattaatcaa taccacaagg aatgaaaagt ggtacgtccg
      661 ttagatagtt tgctttcaat accaaaaatc aagataaatg ggtaaatgat cagaaaaacc
      721 tccttgatat ttcataccga cataggttct gaaaggttgt ttacctaaat tggttttatc
      781 atcttccaat aaaaaatcag cttcaaaaaa atgagcatcc attggcaaag tataaaattt
      841 tcctccagcc tttaataagg ctcctgaaac aataaattga tccagcgcac cccactcacc
      901 attatattta tgagagcctt tgccttttat atgcaaagga tacataaggt tatataaatt
      961 ttctgaataa atagagtcgc ttggaggaag tgctctcaaa gtttttaata aacttttatt
     1021 ggttggataa tcattaaaat ctcccatgat aacaatgtaa ggatgatttt gcacctgata
     1081 aatgctatca atcgttgttc gtaaaataga tgccacttct actcttttgt tttcagattc
     1141 caattctcct cccagtcgcg aaggaaaatg acaaacaaac acatgcaatg tatcatccgt
     1201 tggaattatt cctgcagcat aaagaatgtc acgtgttttt atctgtggag aagttgaaaa
     1261 atgaatggga ataggtttac tgatcaatgg tttgaaaaat tgcggttgat aaagaagtgc
     1321 tacatcaatt ccacgtggat caggtgattc aaaatgcaaa aatttgtaat gtaaattttt
     1381 taaaccagaa taatgtgtca gatctattag acacctttca ttttctactt cagctaaacc
     1441 taccaaagcg ggtgcattcc aaccaccaac agaagcgatt actttaccaa tattattttg
     1501 ttttttctta tattttgcaa aagtccaacc tctcgttcca tctggcaaaa attctgcatc
     1561 atttgtaaga gaatcatcaa aacaatcaaa aaaattttca gtattataaa aaacaacttt
     1621 aaaattctgt ttttcttgcg gttgagtaaa acctgttaga aaaaaaattg ataatatttc
     1681 gataacaaac acctttttca tatagatgca ttactcagtt ggtttccatt cgtaagctcc
     1741 tgcatctggt ttgccatcgg ctaagcgatt attcccattc aaatccagag gacaatattc
     1801 agttgctatg gtatagtcgc ctaaatcacg aataggtgaa atagaatcag gttcaaaatt
     1861 gaaataatta tttttcttgt aatcataata aatattttta aacactgtgt cgtttttctc
     1921 ataccaacgg atattggtaa attgaggtaa atccaaaggt tcatattttc tgatgtagtt
     1981 atttgaaaat gttcctttgt actcgtccaa aaaacgcgtt accagactga attcattttc
     2041 taaagttcct gttatgacgg aatttttaat tgctgtatgc atgggagcca ctcgattcag
     2101 cgtcataatc ataaatgcag gatcgcttcc ccttgacact ggttgaacat tgctgttatt
     2161 gaaataattt acaatggtgc aatgaataaa agaatgagta ccaccattca aatatacgca
     2221 ataactgctt gtattgctta tttcggtatt gattacgtcc acatttgcat tttgaataac
     2281 taaaccatat cgtaaaaaat tatgaatata acagctattt atttctagat gaggaagaga
     2341 attaatattt tgattcgata aataaatgcc tacatagcca ctattcattt tcacatgacg
     2401 gagcaaatgt tctccatttt cacctaataa ataaattcct ccccattgtc cagctacaac
     2461 gttgtatgga ataggtgttt caaaattaat tttatcaaaa cggtcgccac gcataagaat
     2521 gggatggtca aaagtacctt ctgctcttaa atttccataa acaataagat tggcattatg
     2581 atgaaaataa aattttgttc ctgcttcaag cgttaatgtg cgagcggtat ctattatcag
     2641 attaccataa attaaataag gttttttagc cgttaataaa gtatcgttcc agatgatttt
     2701 atctcgaaaa atttccactt cttgaccata agcttgaagt tggatttgtt ttacaatacc
     2761 atttaccaaa aaaacaattt tatcttccat taaaacaggt acatcagaat ctgtagcatc
     2821 gatggtaact tctacaaaaa tataaatact gtcatgagct ggaatctcta cattggtaaa
     2881 gaaattgttt tcattaacgg ctccatctac attgatgcga aaaaaagaat ttcctccttg
     2941 agccagtgca attgtactga tacggacggc tttgctatta tggttataaa ccattacttt
     3001 cgccgtagta cttccaatcg tagtaaaaag tgtatcgaaa gtgatggtat cggtggaaaa
     3061 ttcaatggta tgtaaaggat tagtggtaaa attgtctttt tcacaagata aaaagttcag
     3121 catgagaaaa atagcaaggg gaaaaaagaa aaaatatttt ttgtgaaaag agaacataat
     3181 gaaatgcatt ataaaatgtt actgatttat ttaggcaaat ttacactaaa agatgaatta
     3241 ctcctaataa aaatttatat ttgtagcaca atattaaatt aaaaaattaa aaaatatggc
     3301 aagtcgaaga aaattgaaaa aaagaatcaa tgaaatttct tctaatttat taggtgaaat
     3361 ttatctgcgt tatttgttgt tcgatattca ggatgaagaa aaatttaatc aattgttatc
     3421 taatatttta ttgatgagaa aagaatttat ttcaaaaatc aatcacattg atgggaaaaa
     3481 taatcctgta atggtaaaga aatatttccg acaatttttt tctgattggg gcacaaaaat
     3541 tactgaaatt attgaagaaa ttgaaaaatt aagtcctgta aaacaggaaa gtgaaaaata
     3601 atctttttct tcactttatt gcgtaatttg tctaaccaaa aagttgcatt tgtgacttga
     3661 attcagtttg attaaaaatt gctttattca aaattaaaac ataaggtcaa catttttgaa
     3721 gtcaatttag acaatacatt attgtgtaat ttatcttgct cagtcaagcc acctgcatct
     3781 ctttccttta aaggagttaa gatattatta aaccccagag caaatttcaa ttcgggagca
     3841 agcttaaaga atgaaaaata caaatcgcag cctacgccaa attcaacata ataatctgtt
     3901 ggttttaata aaatagcttt ttctttgttt cgtccaaaat cataataaat ccccccaccg
     3961 ccaagtaaat aaggacgata attatctttt ctttcagcac tatatttgat atataaagga
     4021 aggcatagag gaattgaatt aatatttgta gaagtagtaa tagaagaggt atcagcccga
     4081 taagttaaag ttctttccga aaaatgaaga gtaggagtaa aacgaagatt ccagtattta
     4141 cttaatcgta agttagttat tatgcctacc gaaaatcctg gctgcagtgt tgacactgaa
     4201 acgtggtaag ctttattatc aataagcgta tctttatgta cgattccaaa gtctgcggta
     4261 ttcaatccta ctaaaaatcc gaaatgataa ggtttatcat ccacataggg caaattacct
     4321 tgtgcaaaaa ttatcataaa aggaaaaagg aagaaaaaaa gaaaaagatt tttttttctt
     4381 gacgagaaaa ttagtaaaat ttttatagca tctttcaaat aaagaatatt atttaacttt
     4441 ttgtattgaa tcatttgata ataaataaat taattaaaaa aagaaaaata taaaagctac
     4501 tactttttct ttattttgaa aaaacgctta aaataaatga ttattgttta tacttttaca
     4561 agtatacaaa agaagataat aaaaaacctc tttataaact taagcacttg acaatttcag
     4621 ttttttaatt atcacttaac acaataggtg agatttttta ataaattttt caaattacta
     4681 ttagtaaaaa ccgatgaaga ttttttaaga taaaagtaat tcttagaatt atttagtaaa
     4741 aataatagta gtatgtaatt atctggcttt gtgtcaaccc attaaatctc ctaatttttc
     4801 gaccatagct atcatataaa tctttatcta taatagttgc catttttctg cccagagcat
     4861 cgtataaatt atttccatct actttaccaa ttaatttacc tttttcatca taaatataaa
     4921 cgccatccac tttccccatt aattttcctg cattatcgta aacaaaaacg ccttctactt
     4981 ttcccattaa ttttcctccg gaattataaa agcaattttc atcaactttt gccactaatt
     5041 ttcccgcagc attataaatt tgctgtgcca ttaggaatga attagtaaat aaaaataaaa
     5101 taagcaaaat ttttttcata gaatgaaaaa atattatttt ttacaaagaa attaaaaata
     5161 atctatttcc ccaaataatt tttatttttt atcactactg accgacaaaa gataagataa
     5221 aaaaggaatg gacaagatga aagtctcgcc cccgggttgt aaatttgtat ttca
//
LOCUS       DAOTKS010000075         5264 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_13992_length_5264_cov_4.409868, whole genome shotgun sequence.
ACCESSION   DAOTKS010000075 DAOTKS010000000
VERSION     DAOTKS010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5264
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13992_length_5264_cov_4.409868"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..457
                     /locus_tag="P5236_02405"
     CDS             158..457
                     /locus_tag="P5236_02405"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRU63245.1"
                     /translation="MGAFDNSGRPTVALDAFKEAAASVISPKNKPKWIFKPNTHTLLF
                     ENKQERISIFQMNGRLVNSFFATNTISLNDLQNGIYIVKAVSYKNEKPEIFKLVK"
     gene            581..1894
                     /locus_tag="P5236_02410"
     CDS             581..1894
                     /locus_tag="P5236_02410"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRU63246.1"
                     /translation="MKVLKFGGTSVGSAERMKKVAELISNDEQKIVVLSAMSGTTNSL
                     LEIADYFYKNNVDGALEHIIALENKYFDVVEELYQTEKKKSEAKDILKSHFDYLRSFA
                     NAVFTLFEEKAVVAQGELLSTNLFQLYLEEIGENSILLPALDFMRLDKNGEPDTEYIK
                     MHLLELLKANPGFKIYLTQGFICRNCYGEIDNLQRGGSDYTASLIGAAVEAEEIQIWT
                     DIDGLHNNDPRYVKNTKAIPMLNFEEAAELAYFGAKILHPTCILPAKLANIPVRLLNT
                     LQPEAPGTLISTQINKGKIEAVAAKDGITAIKIKSGRMLLAYGFLRKVFEIFESYKTP
                     IDMITTSEVGVSVTIDNTKYLEEIVNDLKKLGTVSVDKDMVIVCVVGDLFYENVGFQA
                     QIVEALKDIPIRMISYGGSNYNISFLIKKEDKGKALNALSDALFN"
     gene            1946..3115
                     /gene="lysA"
                     /locus_tag="P5236_02415"
     CDS             1946..3115
                     /gene="lysA"
                     /locus_tag="P5236_02415"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRU63247.1"
                     /translation="MKAQYPIEKFSKLQTPFYYYDLDILRKTLQEVNKQIQKSDYIVH
                     YAIKANANPALLHEIKDTGFGADCVSGGEINAAIKAGIPASNIVFAGVGKTDWEINLA
                     LDKDIFCFNVESIPELEVINQLAKAKGKITNIALRLNPNINPHTHHYITTGLEENKFG
                     INLSDLDEVMNLLPKLKNLKLIGVHAHIGSQITDMSSFKSLCIRINEIQQYFFARRII
                     LEHVNVGGGLGVNYEHPNHFPIPDFESYFNVFRQHLLLQPQQILHFELGRSIVAHCGS
                     LISRVTFVKKGATKKFAILDAGFTDLIRPALYQAYHRIENLTSDLPEEEYDVVGPICE
                     SSDVFAKNYPMNQARRGDLIALRSAGAYGESMASQYNLRPLPKTYTSDQLLLEDN"
     gene            3173..4333
                     /locus_tag="P5236_02420"
     CDS             3173..4333
                     /locus_tag="P5236_02420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRU63248.1"
                     /translation="MEIFNIYFSRAELYFLGGLLLTFIYQIYFYFHYINGVIHYRKKI
                     QHQKVSFLEKQPPVSVIICAKNEAENLSKFLPAILEQDYPDFEVIVINDGSVDDTDIL
                     LGAFSKKYPNLRTTFVPQEAQNLSTKKLGITLGVKAAKNDLLLFTDADCVPASHQWIK
                     LMVRNFLPDIEFVLGYSGYSSQKGFLNQLITYDTLFIGLQYMGMALAGKPYMGVGRNL
                     AYRKQTFFDQNGFASSLNLLAGDDDLMVNRAANKKNTRVEISPESITWSRPKTTFKSW
                     YQQKKRHLSVSSAYTKKSKWRLMLEPLTRGLFYILLIVTCIVGNNITKIFAVFLFLVR
                     YAIQITIINRSARHFKERQYFLTLLVFDLLLPLIPLSILATNRKLSRYNKKW"
     gene            complement(4342..4896)
                     /gene="rsmD"
                     /locus_tag="P5236_02425"
     CDS             complement(4342..4896)
                     /gene="rsmD"
                     /locus_tag="P5236_02425"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018910058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HRU63249.1"
                     /translation="MRIISGKYKGRKIYAPINISARPTTDFAKEGLFDLLINRIDLEG
                     IDVLDLFAGTGGIGFEFVSRGCNKVVSIETDKNQCAFIRKVVSDLKIDNLFLFRTDVF
                     KYIASTHAQYDMIFADPPYLLKNIAEIPDLIFSYHLLKPNGIFVLEHSAKYNFQHHPH
                     FVEQRSYGKVNFTFFKVNPSKEIF"
     gene            complement(4899..>5264)
                     /locus_tag="P5236_02430"
     CDS             complement(4899..>5264)
                     /locus_tag="P5236_02430"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HRU63250.1"
                     /translation="SIIYEVFEQFFPKLNIEHHITRFLINLQVYTNKEENLNVCLREH
                     LMDVVAIENNRLMLINSFVFQTPEDFVYYLLRVIEELSFDIDSCKIRLFNYEKYHSKL
                     YNLTQKYMKHCEIIEKKRE"
BASE COUNT         1814 a          849 c          868 g         1733 t
ORIGIN      
        1 ttaaaaatta aaactgacaa attctgattt ttgtcggtta atagtgaaaa aatttctttc
       61 cgatctgata acaaaaacaa aaagcgtttc taatggaaaa ggattaggag tgttttattg
      121 agaaccagaa tgttacaata attggaatgg ttatacaatg ggggcatttg acaatagtgg
      181 aagacccacc gtagcacttg atgcttttaa agaagctgct gcttcagtca tttcacccaa
      241 aaataaacca aaatggattt tcaagccaaa tactcatact cttttgtttg aaaataaaca
      301 agaaagaatt tctatttttc aaatgaatgg aagattggta aactcatttt ttgctactaa
      361 tacaatttcg ttgaatgacc tgcaaaacgg catttatatt gtgaaagctg ttagctataa
      421 aaatgaaaaa cctgaaattt tcaaactggt aaaataataa aaatttgcta ctcattaaat
      481 caaagaaaat tactaccttt gcaagtaatt ttaacattct tgcaattatt tttaattgaa
      541 ttataactta ttgatacata atttttaaac aaattatcaa atgaaagttt taaaatttgg
      601 tggcacttct gtgggctcgg ctgaacgaat gaaaaaagtg gcagagctta tttcaaatga
      661 cgaacagaaa attgttgtat tatcagccat gtcaggaact actaacagcc tgctggaaat
      721 tgcagattat ttttacaaaa ataatgtgga tggagcattg gaacatatta ttgctctgga
      781 aaataaatat tttgatgttg ttgaagaatt atatcaaacc gaaaagaaaa aatcagaagc
      841 aaaagatatt ctcaaatctc attttgatta tttgcgttca tttgctaatg ccgtttttac
      901 tttatttgaa gaaaaggctg tagtagcaca aggtgaatta ctttctacca acttatttca
      961 actttatctg gaagaaattg gagaaaatag cattcttttg cccgctctgg attttatgcg
     1021 attggacaaa aatggtgaac ctgataccga atacatcaaa atgcacttat tggaattatt
     1081 gaaagccaat ccaggtttca aaatctacct tacgcaaggc tttatttgcc gtaattgtta
     1141 tggagaaatt gataatttac aacgtggtgg aagtgattat accgcttcat taattggtgc
     1201 agcggttgaa gcagaagaaa ttcaaatatg gacagatatt gatggtttgc acaacaacga
     1261 cccgcgttat gtaaaaaata caaaagccat tccaatgctt aattttgaag aagcagcaga
     1321 attagcttat tttggagcca aaattttgca tccaacatgt attctgcctg caaaacttgc
     1381 caatattcct gtgcgtttac tcaatacatt acagcctgaa gctccaggaa ctcttatttc
     1441 aacacaaatc aataaaggta aaattgaagc agttgctgca aaagatggta tcacagccat
     1501 taaaatcaaa tcaggaagaa tgctcttagc gtatggtttt ttgcgaaaag tgtttgaaat
     1561 tttcgaatca tataaaacac caattgacat gatcactact tcagaagttg gtgtttctgt
     1621 aacaattgac aatacaaaat atttagaaga aattgtaaac gacttaaaaa aattaggaac
     1681 tgtttccgta gataaggata tggttattgt ttgtgtggtg ggtgacttat tttatgaaaa
     1741 cgtaggtttt caagctcaaa tagtggaagc tttaaaggat atacctatcc gaatgatttc
     1801 ttacggcggc agtaattaca atatttcttt tctcattaaa aaagaagata agggtaaagc
     1861 gctgaacgct ttaagtgatg ctcttttcaa ttaaaaaagt aatgttgata taaaaagtat
     1921 tggctctttc tttttaaaaa ttatgatcaa agcacaatat cccatagaaa aattttcaaa
     1981 attacagact ccgttttatt attacgatct ggatattcta cgcaaaactt tacaggaagt
     2041 gaacaagcag attcaaaaaa gtgattatat tgttcattac gccataaaag ccaatgctaa
     2101 tccggcttta cttcacgaaa taaaagatac aggatttgga gcagactgtg taagcggagg
     2161 agaaataaat gctgcaataa aagcaggtat tcctgcttca aatattgtgt ttgctggggt
     2221 tggaaaaaca gattgggaaa taaatcttgc attggacaaa gacatttttt gtttcaatgt
     2281 agaatctatt cccgagcttg aagtaattaa tcagttagcc aaagcaaaag gaaaaattac
     2341 aaacattgct ttgcgtctca atccaaacat caatcctcat acgcatcact atattaccac
     2401 aggattggaa gaaaataaat ttggcataaa tctttccgat ctggacgaag taatgaattt
     2461 gcttcctaaa ctgaaaaatt taaaactcat tggcgttcac gcacacattg gctctcaaat
     2521 aaccgatatg agctctttta aaagcttatg cataagaatc aatgaaattc agcaatattt
     2581 ttttgcaaga agaatcattc tcgaacacgt aaatgttggc ggtggattag gtgttaatta
     2641 cgaacaccct aaccattttc ccattcccga ctttgaaagt tattttaatg tatttcggca
     2701 acatttgctg ttgcagccgc aacaaattct ccattttgaa ttgggacgat ccattgttgc
     2761 ccattgtggc agtttgattt caagagtaac ttttgtaaaa aaaggagcta caaaaaagtt
     2821 tgctattctg gatgctggtt ttaccgattt aattcgtcca gcgttgtatc aagcttatca
     2881 tcgcattgaa aatcttactt ccgatttacc ggaagaagaa tacgatgtag tgggaccgat
     2941 ttgcgaatcg tccgatgttt ttgcaaaaaa ttatcccatg aatcaagcta gaagaggcga
     3001 tttgattgct ttgcgttctg ctggtgctta tggcgaaagc atggcttcac aatataattt
     3061 gcgaccttta ccaaaaacat atacttccga tcaactttta ttggaagata attaagatat
     3121 taataaacaa tgattgtcca ataaatctat tttattctgt aatgtttttt caatggaaat
     3181 atttaacatt tatttttctc gggctgaatt atatttttta ggtggattac tattaacttt
     3241 tatttatcag atttattttt atttccatta catcaatggc gttattcatt acagaaaaaa
     3301 aattcaacat cagaaagtat cttttctcga aaaacagccg cccgtttcgg taattatttg
     3361 tgcaaaaaat gaagcggaaa atttaagcaa atttttgcct gccattttag aacaggatta
     3421 tcctgatttt gaagttattg tcattaatga tggttctgtt gatgataccg acattttgtt
     3481 gggagcattc agtaagaaat atcccaactt acgaaccact ttcgttccac aggaagcaca
     3541 aaacctcagc accaaaaaat taggaattac gttaggtgta aaagctgcaa aaaatgattt
     3601 gcttctcttc accgacgccg actgcgtacc agcaagccac cagtggataa aattaatggt
     3661 gcgaaacttt ttacctgata ttgaatttgt tttaggatac agtggttatt cttctcaaaa
     3721 aggttttctc aatcaattaa ttacttacga tacgttattt attggtttgc aatatatggg
     3781 catggcactg gcagggaaac cttatatggg agtaggtaga aatctggcat acaggaagca
     3841 aacttttttt gatcagaatg gctttgcttc cagtttaaat cttcttgctg gcgatgatga
     3901 tctgatggta aaccgggctg ccaataaaaa gaatactcgt gtagaaattt cccctgaaag
     3961 tattacctgg tctcgaccaa aaactacttt caaaagctgg tatcagcaaa aaaagcggca
     4021 tctctccgta tcatcggctt atactaaaaa aagtaaatgg agattaatgt tagaacctct
     4081 gacacgtggt ttgttttata ttttattgat tgttacttgt attgttggaa ataacattac
     4141 aaaaattttt gctgtttttc tatttttggt tcgatatgca attcaaatta ctattataaa
     4201 tcgttcagcc cgacatttta aagaacgtca atattttctt acgctgcttg tttttgatct
     4261 tttgcttccc ttaattcctt tgtctatttt agcaacaaac agaaaattaa gcagatataa
     4321 taaaaaatgg tagtaaaaaa atcaaaaaat ttcttttgaa ggatttactt taaagaaagt
     4381 aaaatttact tttccgtagc ttcgttgttc tacaaaatga ggatggtgtt ggaaattgta
     4441 ttttgccgaa tgttcaagca cgaaaatccc attgggcttt agcaaatgat aagaaaaaat
     4501 aagatcggga atttcagcaa tatttttcag taaataagga ggatcagcaa aaatcatatc
     4561 atattgagcg tgtgtagagg caatatattt gaaaacatct gttctgaaaa gaaaaagatt
     4621 atcaattttc aaatcactga caacttttct aataaaagca cattgattct tatctgtttc
     4681 aatgctcact accttattgc agccacgcga aacaaattca aatccaatac ctccagtccc
     4741 tgcaaacaaa tccagcacat caattccttc taagtcaatt ctatttatca ataaatcgaa
     4801 caagccttcc tttgcaaaat cagtagtagg tcgcgctgaa atatttatcg gggcataaat
     4861 tttcctacct ttatattttc cactgataat tctcatattt attccctctt tttctctata
     4921 atttcacaat gcttcatata tttttgtgtt aaattatata attttgaatg atatttttca
     4981 taattgaaca atcttatttt acaactatca atatcaaaag aaagttcttc tattacccgc
     5041 aatagataat aaacaaagtc ttcaggagtt tggaatacaa aactatttat taacatcaac
     5101 cgattgtttt caatagcaac tacgtccatt aaatgctctc gaagacacac attcaaattt
     5161 tcctccttat ttgtatagac ttgtaaatta attaaaaatc gagtaatatg atgttcaata
     5221 ttcaactttg ggaaaaattg ttcaaaaact tcatatataa tgct
//
LOCUS       DAOTKS010000076         5239 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_14059_length_5239_cov_4.687886, whole genome shotgun sequence.
ACCESSION   DAOTKS010000076 DAOTKS010000000
VERSION     DAOTKS010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14059_length_5239_cov_4.687886"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="P5236_02435"
     CDS             complement(<1..175)
                     /locus_tag="P5236_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005825680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L17 family ribosomal protein"
                     /protein_id="HRU63251.1"
                     /translation="MRHNKKFNHLSRKAAHRKSMLANMACSLIFHKRIATTVPKAKAL
                     RVYIEPLLTKAKSD"
     gene            complement(181..1173)
                     /locus_tag="P5236_02440"
     CDS             complement(181..1173)
                     /locus_tag="P5236_02440"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRU63252.1"
                     /translation="MAILAFQKPENVIMLEYGDFHGKFEFRPLEPGYGITIGNALRRV
                     LLSSLEGFAITSIKIEGVDHEYSTIPGVIDDVTNIILNLKQVRFKQIVEGVDNEKVTI
                     KVSNTTAFKAGDIGKYLTGFEVLNPDLLICELDESASFQINITINKGRGYVTAEENRL
                     PNVEFGVIPIDSIFTPIRNVKYQIENYRVEQVTNYEKLVIEVDTDGSIHPKEALKEAA
                     MILIQHFMLFSDEKISVEIEEREESEEFDEEILRMRQLLKTKLVDLELSVRALNCLKA
                     ADIETLGQLCSYQKHDLLKLRNFGKKSLSELEEKLESLNLTFGMDVSKYKLDKE"
     gene            complement(1189..1794)
                     /gene="rpsD"
                     /locus_tag="P5236_02445"
     CDS             complement(1189..1794)
                     /gene="rpsD"
                     /locus_tag="P5236_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRU63253.1"
                     /translation="MARYTGPKSKIARKFGEAIFGADKVLTRKNYPPGQHGNMRRRKT
                     TEYGIQLREKQKAKYTYGLLEKQFRLTYEKALRNPGITGIILLQLLESRLDNIVYRFG
                     MAPTRAAARQLVSHKHIVVDGKVVNVPSYQVKPGQIVGVREKDKAMEVILNSLNGFNH
                     SKYPWLEWDANTLSGKYLYIPEREDIPENIKEQLIVELYSK"
     gene            complement(1872..2261)
                     /gene="rpsK"
                     /locus_tag="P5236_02450"
     CDS             complement(1872..2261)
                     /gene="rpsK"
                     /locus_tag="P5236_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRU63254.1"
                     /translation="MAKKPVSAKKRVVKIDSVGQLHIHSSFNNIIVTITNEEGQVISW
                     SSAGKMGFRGSKKNTPYAAQVAAQDCAKVAYDLGVRKAKAFVKGPGNGRESAIRTVHS
                     SGIEITEIIDVTPLPHNGCRPPKRRRV"
     gene            complement(2315..2695)
                     /gene="rpsM"
                     /locus_tag="P5236_02455"
     CDS             complement(2315..2695)
                     /gene="rpsM"
                     /locus_tag="P5236_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRU63255.1"
                     /translation="MAIRIVGVDIPQNKRGEIALTYIYGIGRSSANKILKEAGVDRDI
                     KAKDWTDEQIAKIREIISSNYKVEGDLRGEVQMNIKRLMDIGCYRGVRHRIGLPVRGQ
                     STKNNARTRKGRKKTVANKKKATK"
     gene            complement(2712..2828)
                     /gene="ykgO"
                     /locus_tag="P5236_02460"
     CDS             complement(2712..2828)
                     /gene="ykgO"
                     /locus_tag="P5236_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018106937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HRU63256.1"
                     /translation="MKVRVSIKKRSEDCKIVRRKGRLYVINKKNPRFKQRQK"
     gene            complement(2840..3058)
                     /gene="infA"
                     /locus_tag="P5236_02465"
     CDS             complement(2840..3058)
                     /gene="infA"
                     /locus_tag="P5236_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRU63257.1"
                     /translation="MAKQTAIEQDGTIIESLSNAMFRVELENGHIITAHISGKMRMHY
                     IKILPGDKVKVEISPYDLTKGRIIFRYK"
     gene            complement(3069..3854)
                     /gene="map"
                     /locus_tag="P5236_02470"
     CDS             complement(3069..3854)
                     /gene="map"
                     /locus_tag="P5236_02470"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRU63258.1"
                     /translation="MIFLKSEEEIELLRISNQLVAKTLAELAKHIVPGISTLELNNIA
                     DEFIRDHGAIPNFLNFNGYPYSICTSVNEQVVHGMPSSKVILKEGDIISIDCGAYLNG
                     FHGDSAYTFCVGEVDPEIIKLLRTTKESLYKGIEQAQEGNRIGDISFAIQSYCEERGY
                     SVVREMVGHGIGRDMHESPEVPNYGRKGNGIMLKTGMTICIEPMINMGTRHIVIEQDG
                     WTTRTADRKPSAHFEHAVVIHHGKTEILSSFKYIEEVLADKAI"
     gene            complement(3869..5212)
                     /gene="secY"
                     /locus_tag="P5236_02475"
     CDS             complement(3869..5212)
                     /gene="secY"
                     /locus_tag="P5236_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRU63259.1"
                     /translation="MNKFIETLKNIWKIEDLRSRLLTTLLFVLIYRFGSFIVLPGIDP
                     ASLTALKAQTSGGLMALLDMFSGGAFANASIFALGIMPYISASIVIQLLTIAVPYFQK
                     MQREGESGRRKMNQITRYLTVAILLLQGPSYLVNLNMQLRQTGVALPEGFWFQASSTI
                     ILCAGSMFVMWLGERITDKGIGNGISFIILVGIIARFPNAIVAEFASRVTDESGGLIM
                     FLTEIIFLLIVIGASIMLVQGTRKIPVQYAKRIIGNKQYGGVRQYIPLKINAAGVMPI
                     IFAQAIMFIPITIVGFSKSESMRGFIGAFMDNNSFWYNFVFVILIILFTYFYTAITIN
                     PTQMAEELKRNNGFIPGVKPGKHTAEYLDIIMSRITLPGSIFLAIIAIMPAFASIFGV
                     NRSFAQFFGGTSLLILVGVILDTLQQIESHLLMRHYDGLLKTGRVKGRTGGVSAY"
BASE COUNT         1660 a         1012 c          717 g         1850 t
ORIGIN      
        1 tatcactttt cgcttttgtt aaaagtggct caatatatac tcgtaaagct ttggctttag
       61 gaacagtagt ggcaattctt ttgtgaaata tcagtgaaca ggccatattt gccagcattg
      121 attttcgatg agccgctttt cgacttaaat gattgaattt tttattatgt ctcatctttt
      181 ttactcttta tctaatttat acttagaaac atccatccca aaagtaagat ttaaactttc
      241 taatttctct tctaattcgc taagtgattt tttgccaaaa tttctcagct ttaacaaatc
      301 atgtttctga taactacata attgacccaa cgtttcaata tctgctgctt ttaaacaatt
      361 gagagcacga acagataact caagatctac taattttgtt tttagcaatt ggcgcattct
      421 caaaatttct tcatcaaatt cttcactttc ttctctttcc tcaatttcta cagaaatttt
      481 ttcatcagag aaaagcataa aatgctgaat taatatcatt gctgcttctt ttaatgcttc
      541 ttttggatga attgaaccat cagtatccac ttcgataact aatttttcat aatttgttac
      601 ttgttcaacc ctgtaatttt ctatttgata ttttacattt cttataggag taaaaattga
      661 atcgatagga attactccaa attcaacatt gggtaatctg ttttcttcgg cagtcacata
      721 accacgtcct ttattaattg taatattgat ttgaaaactt gcgctttcat caagttcaca
      781 aataagaaga tctggattaa gcacttcaaa gccagttaga tattttccaa tatctccagc
      841 tttaaaggca gtagtatttg aaaccttgat agttaccttt tcgttatcga caccttcaac
      901 aatttgctta aaacgaactt gttttaagtt aagaatgatg ttggtcacat catcaatcac
      961 acccggtata gtagaatatt catgatctac gccttcgatc ttgattgaag tgatggcgaa
     1021 accttccaaa gaggacaata gaactcgtcg caaagcatta ccaatagtaa tgccataacc
     1081 aggttctaaa gggcgaaatt caaattttcc atgaaaatca ccatattcta acatgataac
     1141 attttcgggt ttttgaaatg ctaatatagc catgaattaa attttttatt atttagagta
     1201 taactcaaca attaattgtt ctttaatatt ttctggaata tcttctctct caggaatata
     1261 caaatattta cctgataaag tattggcatc ccattccagc caaggatatt tactatgatt
     1321 aaatccattc aatgaattta gaattacttc catagcttta tctttttcgc gtaccccaac
     1381 aatttgaccc ggtttaactt gataagaagg gacatttaca actttgccat ccacaacaat
     1441 gtgtttatga cttacaagtt gacgggcggc tgctcgtgta ggagccattc cgaaacgata
     1501 aacaatatta tccagacggg attccaacaa ttgcaaaaga ataattcctg tgatacctgg
     1561 gttccgtaaa gccttttcat aggtaagacg gaattgtttt tctaataaac cataagtata
     1621 ttttgctttc tgtttctctc gtaattgaat accatattcc gtagttttcc gccttcgcat
     1681 attaccatgt tgaccaggag gataattttt tcgtgttaaa actttatcag caccgaaaat
     1741 tgcttcacca aatttacgtg cgatttttga ttttggtcca gtatatcttg ccatttttta
     1801 tgctttaaaa atataaaatt aaatcttaac aaataatcac tattttctgt aatttattgt
     1861 atattaaata tttaaactct tcttcttttg ggtggacggc aaccattatg aggaagtgga
     1921 gttacatcaa taatttcggt tatttcaatt ccagaactat gtactgtgcg gatagccgat
     1981 tcacgaccat tgcctggacc ttttacaaaa gctttagctt ttctaactcc taaatcatag
     2041 gctacttttg cacaatcctg agctgcaact tgggcagcgt aaggagtatt ttttttagaa
     2101 cccctaaaac ccattttacc tgctgatgac catgaaatta cctgaccttc ttcattcgta
     2161 attgttacaa taatattatt gaatgaagaa tggatatgta attgacctac actatctatt
     2221 tttacaactc tttttttggc tgaaactggt ttttttgcca tattaattta attttttctt
     2281 tctcagttaa attattattt ttttaaatta attattattt agttgctttc tttttattgg
     2341 caacagtttt ctttcttcct tttcgggttc gtgcattatt ctttgtactt tgacctctta
     2401 ctggtaaacc aatacgatga cgaactcctc gataacaacc tatatccatc aaacgtttga
     2461 tattcatttg cacttctccc ctcaaatctc cttctacttt ataatttgaa ctaattatct
     2521 cgcgtatttt agcaatttgt tcatctgtcc aatctttggc tttaatgtct ctatctacgc
     2581 cagcttcttt taaaattttg ttagcactac tccgacctat tccataaata taagtcaaag
     2641 caatttctcc tcttttgttt tgtggtatat ctacacctac tattcttata gccatatgtt
     2701 aatcttttaa attatttttg acgttgcttg aatctcggat tctttttatt aatcacataa
     2761 agtcgccctt ttcgacgaac aattttacaa tcttctgaac gcttttttat tgaaactcta
     2821 actttcattg ttattctatt tatttatatc taaaaataat tcttcctttt gttaagtcat
     2881 aaggagatat ttcaactttt actttgtcgc ctggtaaaat tttaatataa tgcatacgca
     2941 tctttccaga aatatgagcg gtaatgatat gaccattttc taattcaacg cgaaacatgg
     3001 cattcgacaa tgattcgatt atcgttccgt cttgttctat tgctgtttgt ttggccataa
     3061 attaaccttt aaattgcttt atcagctaat acttcttcta tatatttaaa actcgataaa
     3121 atttctgttt tcccgtgatg gattactaca gcatgctcaa aatgagctga tggttttcga
     3181 tctgctgtac gagtagtcca tccatcttgt tcgataacta tatgacgggt tcccatatta
     3241 atcatgggtt ctatacatat tgtcatacca gttttcagca taattccatt tccttttcgt
     3301 ccataattag gaacttcagg agattcatgc atatcgcgtc caattccatg tccaaccatt
     3361 tcgcgaacta ccgaataacc gcgttcttca caataacttt gaattgcaaa actaatatca
     3421 cctattcgat tcccttcttg agcctgttcg attcctttat ataatgattc cttcgtagta
     3481 cgaagtaatt ttattatttc aggatccact tctcccacgc aaaaagtata agccgaatcg
     3541 ccatgaaaac cattcaaata agctccgcaa tcaatcgaga taatatctcc ttcttttaaa
     3601 attacttttg aagaaggcat tccatgtaca acttgttcat ttaccgaagt acaaatagaa
     3661 taagggtaac cattaaaatt aagaaaattt ggtattgccc catgatccct gataaattca
     3721 tcagcgatat tgtttaattc taaagtactt atacccggaa caatatgttt tgcaagttcg
     3781 gctaacgttt tagccaccaa ttgattgctt atgcgaagta actcgatttc ttcttcagat
     3841 tttaaaaaaa tcatctcaaa taataaattt aataagcaga gacccctcca gtacgacctt
     3901 ttactcttcc agtcttcaat aagccatcat aatgtctcat caataaatga ctttctatct
     3961 gttgcaaggt gtctaaaata actccaacca aaatcagcaa ggaagttcct ccaaaaaatt
     4021 gagcaaacga acgattcaca ccaaaaatac ttgcaaaagc aggcataatt gcaattattg
     4081 ccaagaatat tgagccaggc aatgttattc gagacataat tatatctaaa tattctgctg
     4141 tatgtttacc aggtttaact cccggaataa aaccattatt acgttttaac tcctcagcca
     4201 tttgggttgg attaatcgtg atggcggtat aaaaataagt aaataaaata atcaaaatta
     4261 caaaaacaaa attataccaa aagctattat tatccataaa tgctccaata aaacctctca
     4321 tagattcaga ttttgaaaaa cctacgatag taataggaat aaacataata gcttgtgcaa
     4381 atataatagg cattactcct gccgcattaa tttttaaagg aatatattgt cttacacctc
     4441 cgtattgttt attgccaatt atacgttttg cgtattgtac tggaattttt cgtgtccctt
     4501 gtaccagcat aatagaagct ccaataacaa ttaacaaaaa tataatctct gttagaaaca
     4561 tgattaagcc tccgctttca tcagtaactc gtgaagcaaa ttctgcaaca atagcatttg
     4621 gaaacctcgc aataattcct actaaaatga taaatgaaat tccatttccg atacctttat
     4681 cggtaattct ttctcctaac cacattacaa acatactgcc agcacataaa ataatggttg
     4741 aagaagcctg aaaccagaat ccttcaggaa gtgcaacacc tgtctgtctt aactgcatat
     4801 tcaaattgac taaataagaa ggaccttgta aaagtaaaat agcaacagta agataacgag
     4861 taatctggtt cattttccga cgcccgcttt ccccttcacg ctgcattttt tgaaaataag
     4921 gaacagctat tgtcaacaat tgaattacaa tagaagcaga aatatagggc atgataccca
     4981 aagcaaaaat agaagcatta gcaaaggcac ctccagaaaa catatccaat aaagccatca
     5041 accccccact tgtctgagct tttaaagcag ttaaggaagc aggatcaata cctggaagaa
     5101 caataaaaga accaaaacga taaattaata caaataataa tgttgttaat aagcgacttc
     5161 ttaagtcttc tattttccaa atatttttaa gggtttcaat gaatttattc atccttattt
     5221 aattttatag gagttccac
//
LOCUS       DAOTKS010000077         5238 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_14063_length_5238_cov_4.788347, whole genome shotgun sequence.
ACCESSION   DAOTKS010000077 DAOTKS010000000
VERSION     DAOTKS010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14063_length_5238_cov_4.788347"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="P5236_02480"
     CDS             complement(<1..1181)
                     /locus_tag="P5236_02480"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRU63260.1"
                     /translation="MAEIDDFTFSYSLVRGYVMFAFKRFFSKYIVLGRENLPKEGPVI
                     LAVNHLNALMDALAVISVVPHKMPVVFLARSDYFNNKTIAKFLKFCKIMPAFRMREGI
                     ENIDKNNIVFDKCVEILHHNKVIGIMPEGNQGSQRKIRPLAKGIFRIAFQAQEKYDSQ
                     LAVKIVPVGIDWGNIDKYGEHVIINIGKPIEVADYMSEYEENPVTAINKIRDELKNRL
                     SELTLDIATNENYNCFETATKVANAAMVEKLQLPNKEPFLFFARQKTAQRLLELEQTH
                     PELVEKLTMLCKEYNENLKKLNLPSWLFNKTIKTSSLVLESLLLLVSLPLFLIGFILN
                     LLPFFTPDLIIKAFHLNFPGGRGSLRFGIGLVTFPLFYILQAILFYKKLKATWWATIL
                     F"
     gene            complement(1317..>2569)
                     /locus_tag="P5236_02485"
     CDS             complement(1317..>2569)
                     /locus_tag="P5236_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRU63261.1"
                     /translation="LTLFVLLLLPFSGWLIGKIGKSLKQRSTLGQQQTGELLSQIEET
                     LSGLRVIKAFNAEKSFQNKFAQLNEKIRRTFNRINRRYNLAHPLSELVGTIVIAIVLW
                     FGGVLILENHSSINAAEFIYYLIIFYSIITPAKDLSKAGYSIQKGLASLERIDRILTT
                     PNTIKEPASPMPMKPFSDKIEFRNISFRYKTEWVLRDINITIPKGKTIALVGQSGSGK
                     STLLDLLPRYYDPQKGEILIDGVNIKSLRTSDIRAQIGNVNQEAILFNDTFFNNIAFG
                     SENVTMEQVIEAAKIANAHDFIMATEKGYETTIGDRGSKLSGGQRQRVSIARAILKNP
                     PILILDEATSSLDTESERMVQEAIEKLMQNRTTLVVAHRLSTIKRADEICVIYEGRIV
                     ERGKHEELLALNGYYKRLYDMQSF"
     assembly_gap    2570..2669
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2670..2928)
                     /locus_tag="P5236_02490"
     CDS             complement(<2670..2928)
                     /locus_tag="P5236_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRU63262.1"
                     /translation="MFKFFTLLKRFIPPYKKYVVLNLVYNLLATIFSLFSFATIIPIL
                     QILFGIQRANVSYQPWSWNDSLKDIFNALKNNAFYSIEQIVE"
     gene            complement(2965..4308)
                     /gene="purB"
                     /locus_tag="P5236_02495"
     CDS             complement(2965..4308)
                     /gene="purB"
                     /locus_tag="P5236_02495"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRU63263.1"
                     /translation="MNLSPLTAISPIDGRYHSKTKMYASYFSEFALIRCRVQIEIEYF
                     IALCEIPLEQLKQISADIFPMLRNIYQNFQETDAQKIKDIEQITNHDVKAVEYFLKKK
                     FEEIGLSKYKEFIHFGLTSQDINNTAFPFLLKAALEDVYYAELKKLLEELDQLAENWK
                     DIPMLARTHGQPATPTRLGKEIMVFSYRIKEQLELLKQIPISAKFGGATGNFNAHAVS
                     YPAIDWKKFADNFLSDKFGLKREQYTTQISNYDHLSALLDATKRINTVLIDLCRDMWT
                     YISLEYFKQKIKANEVGSSTMPHKVNPIDFENAEGNFEIANAFFNFLSAKLPISRLQR
                     DLTDSTVIRNIGVPFAHVLIATQSLSKGLSKLVLNDTVIKKDLENNWEVVTEAIQTIL
                     RREGYPEPYEALKSLARTNQPITKKTIHDFIENLDVNENVKEELHKITPFNYTGI"
     gene            4536..>5238
                     /locus_tag="P5236_02500"
     CDS             4536..>5238
                     /locus_tag="P5236_02500"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HRU63264.1"
                     /translation="MKRINIHKEGRFILSGLLLLIFCINIIIYLEFPVTVVAINTILS
                     ALVLCFFTFFFRSPKRIFEIDDPSLVVAPADGTVVVIEPTLETEYFGDERMQISIFMS
                     IFNVHANWYPVSGKVIKTSHRGGRHLAAYLPKSSKENEQASIVIETPLKTQILVRQVA
                     GALAQRIVTYAYPGKECHINEHLGFIKFGSRVDLFLPLDSEIFVKVGEKTTGNETIIA
                     RLKENKDDNQGSSTAS"
BASE COUNT         1648 a          816 c          879 g         1795 t
ORIGIN      
        1 aaaaataaaa tagttgccca ccacgtagct ttaagttttt tataaaataa aatggcttgt
       61 aaaatataaa acaaaggaaa agtaaccagt cctattccga atcgtaatga gccacgaccc
      121 ccaggaaaat taaggtgaaa tgccttaata attaaatcag gggtgaaaaa aggcaataaa
      181 ttcagtataa aacctattaa gaaaagtggt aaagaaacta agagtaacaa actttccagc
      241 actaaagaag aggtttttat agttttgttg aatagccatg aaggtaagtt taattttttt
      301 aaattttcgt tgtattcttt acaaagcatg gttaattttt caactaattc aggatgagtt
      361 tgttccaatt caagtaaacg ttgagctgtt ttttgtcggg caaaaaacag aaagggttcc
      421 ttatttggta attgcaattt ttcaaccata gctgcgttag ccactttggt agcagtttca
      481 aaacaattgt agttttcgtt ggtagcaatg tcaagtgtca attcgcttaa acgatttttt
      541 aattcatctc tgattttatt tattgctgtg accggatttt cttcatattc gctcatgtaa
      601 tctgctactt ctatgggctt tccaatgtta atgataacgt gttcgccata tttgtcaatg
      661 tttccccaat ctataccaac aggtacaatt ttaactgcta attgagaatc atatttttct
      721 tgagcttgaa aagcaattct aaaaatccct tttgcaagag gtctgatttt tcgctggctt
      781 ccctggttgc cttctggcat gataccaatg actttattat gatgcagaat ttctacacat
      841 ttatcaaaaa cgatattgtt tttgtctata ttttcaattc cttcccgcat gcgaaaagct
      901 ggcattattt tacaaaattt gaggaatttt gcaatggttt tattgttgaa atagtcagaa
      961 cgagcaagaa aaactacggg cattttatga ggaactactg agataaccgc cagtgcgtcc
     1021 atcaaagcat tcaaatgatt cactgcaaga ataactggac cttctttagg taaattttca
     1081 cgtcctaaca caatatattt gctaaaaaaa cgcttgaagg caaacataac gtagcctctc
     1141 accaggctat aggaaaaagt aaaatcatcg atttctgcca tttttctcta ttaatttatg
     1201 aatttattaa aattgaaata aatgcaacct ttttagatgg tataaggctt ttccactgag
     1261 caaagttaat aattttttag aaaaaatatc gctgaaaggc tttagcaaaa tgttttttag
     1321 aatgactgca tatcgtataa tcgtttataa taaccattca aagccaataa ttcttcatgt
     1381 tttcctcgtt ctacgatgcg tccttcatag atcacacaaa tttcatcggc tcgtttgata
     1441 gtggaaagac gatgagctac tactagagtt gttcggtttt gcatcaattt ttcaatggct
     1501 tcttgaacca ttctttcaga ttcagtgtcg agcgaagaag tggcttcatc aagaataaga
     1561 ataggtggat tttttaaaat agcacgagca atgctgacgc gttgtcgttg tcctcctgaa
     1621 agtttactac cccgatcacc gatggtggtt tcatatcctt tttcggtcgc cataatgaag
     1681 tcgtgagcat ttgctatttt agcagcttct attacttgtt ccattgttac attttcggag
     1741 ccaaaggcaa tattattgaa aaaagtatcg ttaaaaagaa tagcttcttg atttacattt
     1801 ccaatttgag cacgaatatc agaagttcgt agcgacttga tattaactcc atcaattaaa
     1861 atttcacctt tttgtggatc ataatatctt ggtaataagt ctaagagagt tgacttccct
     1921 gaaccagatt gacctaccag tgcaatggtt tttcctttag gaatggtaat attaatatca
     1981 cgcagcaccc actcagtttt gtatcgaaaa cttatatttc gaaattcaat tttatcacta
     2041 aatggtttca ttggcatagg agaagctggt tctttaatcg tgttgggtgt agttaagatt
     2101 ctatcaattc tttccaacga agctaatcct ttttggatac tatatcctgc ttttgataaa
     2161 tcttttgctg gtgtaataat gctataaaaa ataatcagat aataaataaa ttctgcagca
     2221 ttgatggaac tatgattttc taaaattaat acgccaccaa accataatac aatggctatc
     2281 actatggttc ctactaattc gcttaaagga tgagccagat tgtaacgacg attaattcga
     2341 ttaaatgtgc gacgtatttt ttcatttaat tgtgcaaatt tattttggaa acttttttca
     2401 gcattgaaag cttttatcac acgtaatcca ctcagagttt cttcaatttg tgagagtaac
     2461 tcgcccgttt gttgttgtcc taaagtggaa cgttgcttca aagatttacc aattttgcct
     2521 attaaccaac cactaaacgg taataaaagc agtacaaaaa gtgttagctn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ctcaacaatt tgttcaatgg aataaaaagc
     2701 attatttttt aatgcattga aaatatcttt taaactatca ttccagctcc acggttgata
     2761 gcttacattt gctcgttgga taccaaacaa aatttgcaaa ataggaatga tagtagcaaa
     2821 tgaaaataag ctaaaaatag tagccaataa attatatacg agatttaata caacatattt
     2881 tttataaggt ggaataaatc gtttcaggag tgtaaaaaat ttaaacattt ggcaagaata
     2941 atgaatttta ataattcaaa aaaatcaaat cccagtgtaa ttaaaaggag taattttatg
     3001 taactcttct ttcacgtttt cgttgacatc aagattttct ataaaatcat gaatagtttt
     3061 tttggtaatt ggctgattag tacgagctaa ggattttaaa gcttcataag gttcaggata
     3121 gccttcgcga cgaagaatag tttgtattgc ctcagtaaca acttcccagt tgttttccaa
     3181 atcctttttt ataactgtgt cgtttaatac taatttgctc aatccctttg aaaggctttg
     3241 agtagctatt aaaacatgtg caaaaggtac gcctatgtta cgaataacgg tactgtcggt
     3301 taagtcacgt tgtaaacgtg aaatgggtaa tttagctgat aagaagttaa aaaaagcatt
     3361 agctatttca aaattccctt cggcattttc aaaatcaatt ggattgactt tatggggcat
     3421 ggtcgatgaa ccaacttcat ttgctttgat tttttgttta aaatattcca gagaaatgta
     3481 cgtccacata tcacggcaca aatctatcaa aaccgtattg attcgttttg ttgcatccag
     3541 cagagcagat aaatggtcat aatttgaaat ctgtgtggtg tattgttctc gtttcaagcc
     3601 aaatttatca gacaaaaaat tatccgcaaa ttttttccag tcaatggctg gataagacac
     3661 agcatgagca ttaaaatttc cagttgctcc accaaatttg gctgaaatag gaatttgttt
     3721 taaaagttct aattgttctt tgatacgata ggaaaaaacc atgatttctt ttcctaatcg
     3781 ggttggtgtg gctggttgtc cgtgtgttct tgccagcatt ggaatatctt tccagttttc
     3841 agctaattga tccaactctt caagcaattt tttcaattca gcataataaa catcttcaag
     3901 ggcagctttc aaaagaaatg ggaaagctgt gttattgatg tcttgtgaag tgagtccaaa
     3961 atgaataaat tccttgtatt tacttaaccc tatttcctca aactttttct ttaaaaaata
     4021 ttctactgct tttacatcgt gattagtaat ttgttcaata tcttttattt tttgtgcatc
     4081 ggtttcctga aaattttgat aaatatttct gagcattgga aaaatatcag cagaaatctg
     4141 ttttaattgt tctaatggta tttcacataa ggctataaaa tattcaattt caatctgcac
     4201 tctgcaacgt atcaaggcga attcagaaaa atacgaagca tacatcttcg ttttggaatg
     4261 atagcgtcca tcaattggtg aaatggctgt cagtggagaa agattcataa atgtttctta
     4321 gtttaataaa ttaagatatc aggattgtaa gaaatgaaaa aatttaatta agctaatatg
     4381 ttattacttg caaagttaat caattctgtt taataaggaa agttttattt ttgccttgca
     4441 accaagcgtc tttttcatta cttttgtagg cttcattttt acgattttta atcataaaaa
     4501 tttattgaga gttagatatt tttcacttat tctgaatgaa acgcattaat atacataagg
     4561 aaggacgttt tattttatcg ggactgttgc tgttaatttt ttgcattaac attattattt
     4621 atctggaatt tccagtaaca gttgttgcta ttaataccat tctttcagca ttggttttgt
     4681 gtttttttac attttttttt cgaagtccga agcgtatatt tgaaattgat gatccaagtt
     4741 tggtagttgc tccggctgat ggaacggtgg tggtaataga acctactttg gaaacagaat
     4801 attttggcga tgaaaggatg caaatttcta tttttatgtc tattttcaat gtacatgcca
     4861 attggtatcc tgtttcaggg aaagtaatta aaacatcgca tcgcgggggt cgtcatttgg
     4921 cagcttattt accaaaatcg agtaaagaaa atgaacaagc atctattgta atagaaactc
     4981 cattaaaaac ccaaattttg gtgagacagg tggctggtgc tttggctcag cgaattgtta
     5041 cttatgctta tcctgggaaa gagtgtcata tcaatgaaca tttgggattt ataaaatttg
     5101 gttcacgggt agatttattt ttacctctgg attcggaaat atttgtaaag gtgggagaaa
     5161 aaaccacagg aaatgaaaca attattgctc gtttaaaaga aaataaagat gacaatcaag
     5221 gaagctcaac agcaagtt
//
LOCUS       DAOTKS010000078         5195 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_14193_length_5195_cov_3.653113, whole genome shotgun sequence.
ACCESSION   DAOTKS010000078 DAOTKS010000000
VERSION     DAOTKS010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14193_length_5195_cov_3.653113"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="P5236_02505"
     CDS             complement(<1..372)
                     /locus_tag="P5236_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63265.1"
                     /translation="MKKPFSLIVLLLLLLVGCQPALHYPAENENNINLNSLTTLDVGE
                     NIIYLQDFILNTALIDSITSSSNYLKLSLTTDKKIALATVFPEMETFVDVQVWIDSVP
                     YSIPCRKTDKIDYLFTFHSQGK"
     gene            complement(397..2241)
                     /locus_tag="P5236_02510"
     CDS             complement(397..2241)
                     /locus_tag="P5236_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HRU63266.1"
                     /translation="MKKFYILFLITMTYLPLSGRQINWIQHLEPAFWWAGMNNPNLQL
                     MIHGTDISHCDFFVDDQDISIERKIVTDNPHYVFLYLNIPKNTQPRKFKLLVKKGKNV
                     QQYEYELKARKENSAQRKGFTSADAIYLIMPDRFCNGNSGNDSVPGYYQGVNRNDPSA
                     RHGGDIEGIISKVPYLADLGITAIWTTPLLEDNDQQYSYHHYACSNYYRVDPRFGTNE
                     DYARLGEICHNYGIKLIIDAVPNHCGITHWWMKDIPSKDWFNTWPQYTSTNYRINVWT
                     DPHASKADLLQLTNGWFDRNMPDLNLKNPLVFDYLLQAYVFWLEYAGIDGIRVDTYPY
                     NDIHVASKWLESIKREYPNITIVGECWLKSPAEIAYYQSGNQNKDGFDSHLDCAMDFC
                     LKDVFAFAFNEKESWNSGMNRFYFHFAQDFVYPSPDLIMNFLDNHDTERYETAINGDV
                     AKYKMALAVLMTVRGFPQIYYGTEIMLAGKVGTYEGQRFDFPGGWLDDPHNAFTAEGR
                     TQTENEIFTYLQTLLHYRKNNLVLQSGEMKHFIPENGIYVYFRYNEDKTVMIVANNNE
                     QNSKLSLGRFSEMTSNKKYGKDIVNGKTYPLNAPIEVSAKTVLILELS"
     gene            complement(2296..4608)
                     /locus_tag="P5236_02515"
     CDS             complement(2296..4608)
                     /locus_tag="P5236_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 65 glycosyl hydrolase domain-containing
                     protein"
                     /protein_id="HRU63267.1"
                     /translation="MNKYLQHDPWCIIEEGFHMDSQLASESIFSLGNGHMGQRGNFEE
                     YYSGETMFGSYIAGIYFPERAERSNWKNGYSETNDKIVNAPNWICISVRLNDERLDLG
                     SWDIHNFRRVLNMREGYLERTFEVTSPKGHRIQATVRRFLSMAENEVAVISYTVKSIN
                     YEGRISFLPLIDGNVRNYYTNYNEPFWNVLQTKTEQDVSHLWAQTRRLDFHVCAALTY
                     EFYKNNMLLNVNPIKIEKERVAGFSVGTDIKKGETVCLKKYVSVMSSLNHPREELTDR
                     ACNLARAVKQKGWDKLFDEHKAVWKEKWLQSDVIIENDIAAQQAIRFNIFQLNQSYSG
                     NDDRLNISPKGFTGEKFGGGTRWDTEAVCVPFYLSTSPQNVARNLLLYRYRHLPKAIQ
                     NAEKLGFTNGAALFPAATFNGSECLNEWEISFEEVFRNGIIAYAIYNYLRYTDDNDFL
                     VNYGLRILIAIARFWAQRVNISHQKKKYVLLGVTGPNTYENNVNNNWFTNFIAVWCLK
                     FTMEAIEYVKKKDFRAFGDLSQSINFSNEEVFLWKDITENMYFPEDKKLNIFLQQDGF
                     FDKTLVKASEIPASQLPLNQHWTWDKILRSPFIKQADVVLGLYLFEDLFDKETIRQNF
                     DFYEPFTVHESSLSPCIHSIVASSIGEIEKGYHLYLRTARLDLDDYNDEVADGLHITS
                     MAGTWLSIVQGFGGMRVIDNHLHFNPHIPEKWEAYAFNVIFRGTQLKIRVEKKKVILQ
                     RIKGEPIDLYVYNNLIHLSGNECEEIERKN"
BASE COUNT         1813 a          947 c          713 g         1722 t
ORIGIN      
        1 tttcccttgc gaatgaaaag taaaaaggta atcaatttta tctgtttttc gacatgggat
       61 tgaataagga acactatcta tccacacctg aacatcaaca aatgtttcca tttcaggaaa
      121 aacagtagcc aaagctattt ttttatctgt tgtaaggctt aattttaaat aattagaaga
      181 acttgtaata gaatcaatta aggctgtatt taaaataaaa tcctgcaaat aaataatatt
      241 ttcaccaaca tcaagggtcg taagagaatt taggttaata ttattctcat tttctgctgg
      301 ataatgtaag gctggttgac agccaacaag caaaagaagc aacaaaacaa ttaaagaaaa
      361 aggtttcttc atagtataaa aattaatatt cttctatcag gataattcta aaattaaaac
      421 tgttttagca gaaacttcta ttggcgcatt taaaggataa gtttttccat taacaatgtc
      481 cttcccgtat tttttatttg aagtcatttc gctaaatcta cccaacgata atttgctgtt
      541 ttgttcatta ttatttgcta caatcataac agttttatcc tcattataac gaaaatacac
      601 atagattcca ttttctggaa taaaatgttt catttcccct gattgcaaaa ccaggttatt
      661 ttttcggtaa tgcaaaagag tttgcaaata agtaaaaatt tcgttttcag tttgagtacg
      721 tccttcggcg gtaaaagcat tatgaggatc gtcgagccaa cctcccggaa aatcaaaacg
      781 ttgaccttca taagttccaa cctttcctgc cagcataatt tcagtaccat aataaatttg
      841 aggaaaacct ctcactgtca tcaaaacagc aagtgccatt ttgtattttg ctacatcgcc
      901 attaatggct gtttcataac gttcagtatc gtgattatcc aaaaaattca tgatcaaatc
      961 aggagaagga tatacaaaat cctgagcaaa atgaaaataa aatcgattca ttccactatt
     1021 ccagctttct ttttcattaa aagcaaaagc aaaaacatcc ttcaaacaga aatccattgc
     1081 acaatctaaa tgggaatcaa aaccatcttt attttgattt cccgactgat aataagcaat
     1141 ttctgctggc gatttcaacc agcattcacc tactatcgtt atgtttggat attctctttt
     1201 aatgctttcc aaccattttg aagccacatg aatatcatta taaggatagg tgtctacccg
     1261 gataccatcg ataccggcat attcaagcca aaaaacataa gcttgtagca aataatcaaa
     1321 cactaatggg tttttaagat tcaaatcagg catatttcta tcaaaccatc cattggtcaa
     1381 ctgtaacaaa tccgctttag aagcatgagg atcggtccat acattaattc tataatttgt
     1441 tgaggtatat tgtggccagg tattgaacca atcctttgaa ggtatatctt tcatccacca
     1501 atgagtaatg ccacaatggt ttggtaccgc atcaataatt agttttatcc cgtaattatg
     1561 acaaatttca cctaaccggg cataatcttc atttgtacca aagcgaggat caacacgata
     1621 ataatttgaa caggcataat gatgataaga atattgttga tcattatctt ccagtaacgg
     1681 tgttgtccaa attgccgtta ttcctaaatc agccagataa ggaacttttg aaataattcc
     1741 ttcaatatca ccaccatgac gagccgatgg atcattgcga ttgactcctt gataatatcc
     1801 tggaacagaa tcatttcctg aatttccatt acaaaatcta tcaggcataa tcaagtaaat
     1861 agcatcagca gaagtaaaac cttttcgttg agcagaattt tcttttcttg cttttagttc
     1921 atactcatat tgttgaacgt ttttgccttt ttttacaaga agtttaaatt ttctgggttg
     1981 agtatttttt ggaatattta aataaagaaa aacataatga ggattatcag taactatttt
     2041 acgttcaata cttatatcct gatcatcgac aaaaaaatca cagtgcgaaa tatctgtacc
     2101 atgaatcata agctgcaagt taggattatt cattccagcc caccagaaag caggttccaa
     2161 atgctgaatc cagttaattt gtctgccaga aagtggcaaa tatgtcatgg tgatcaaaaa
     2221 aagaatataa aattttttca tggttagaaa ttttaatttg tcttgttatt ttcttacgaa
     2281 attctatata taaattcaat tttttctttc aatttcctca cattcattcc ctgaaagatg
     2341 aatcagatta ttataaacat ataaatcaat aggttcgcct tttatgcgtt gaagaataac
     2401 tttctttttc tctaccctaa tttttaattg tgttcctcta aaaataacat taaaagcata
     2461 agcttcccac ttttcaggaa tatgaggatt aaaatgcaaa tgattatcaa tgacacgcat
     2521 accaccaaaa ccttgaacaa tagacaacca tgtaccagcc atacttgtaa tatgcaatcc
     2581 atcagctact tcgtcgttgt aatcgtccaa atcaagacgt gctgttcgta aataaagatg
     2641 ataacctttt tcaatttcgc caatactgga agctacaatg gaatgaatac aaggcgacaa
     2701 ggaagattca tgaacggtaa aaggttcata aaaatcaaaa ttttggcgta tggtttcttt
     2761 gtcgaacaaa tcttcaaaaa gataaagtcc taacactaca tcagcttgct taataaacgg
     2821 tgatcttaaa attttatccc acgtccaatg ttggttaagt ggcaattgcg aagctggaat
     2881 ttcagatgct tttaccaacg ttttatcaaa aaaaccatct tgttgaagaa aaatatttag
     2941 tttcttatct tctgggaaat acatattttc tgtaatatct ttccacaaaa aaacttcttc
     3001 attggaaaaa ttgatacttt gcgacaaatc accaaaagct ctaaaatctt tctttttcac
     3061 atattcaatg gcttccattg taaattttaa acaccataca gcaataaaat tggtaaacca
     3121 gttattatta acattgtttt cataagtatt tggacccgtc acacctaaca agacatattt
     3181 tttcttttga tgagaaatat tgacacgttg agcccagaaa cgagcaatgg caattaaaat
     3241 tcttaatccg taattgacta aaaaatcatt gtcgtccgta tatcggagat aattatagat
     3301 agcataagca ataatgccat tccgaaaaac ctcttcaaaa gaaatttccc attcatttag
     3361 acattcgcta ccattaaaag tagcagcagg aaataaagca gctccattcg taaaaccaag
     3421 tttctcagca ttttgtattg cttttggtaa atgacgataa cgataaagca ataaattacg
     3481 tgcaacgttt tgaggagaag ttgataaata aaaaggcaca caaactgctt cggtatccca
     3541 gcgagttccg cctccaaatt tttcgcctgt aaaacctttt ggactaatat ttaaacgatc
     3601 atcattcccc gaataacttt gatttaattg aaaaatatta aatcgaatag cttgttgagc
     3661 tgctatatca ttttcaataa tcacatcgga ttgcaaccat ttttccttcc aaacagcctt
     3721 atgttcatca aacaatttat cccaaccttt ttgcttaacg gcacgagcta aattacatgc
     3781 tctgtctgta agttcttctc ggggatgatt caaagaactc attacagaca catatttttt
     3841 caagcaaact gtttctcctt ttttaatatc ggtgcccaca ctaaaccctg ctactctttc
     3901 tttttcaatt tttataggat taacattcaa aagcatatta tttttataaa attcataagt
     3961 caatgctgca cacacatgga aatcaagacg tcgagtttgt gcccataaat gagaaacatc
     4021 ctgttccgtt ttagtttgca atacattcca gaaaggttca ttataattgg tataataatt
     4081 tctgacattg ccatcaatca aaggtaaaaa cgagatcctt ccttcgtaat tgatagattt
     4141 cacagtataa ctaataacag cgacttcatt ttctgccatg cttaaaaatc gcctcaccgt
     4201 agcttgaatg cgatgacctt taggagaagt aacttcaaaa gtgcgttcca aatagccttc
     4261 acgcatattt aaaacacgcc tgaaattgtg aatatcccac gaacctaaat ccaatcgttc
     4321 atcatttaaa cgaacactga tacaaatcca gttaggagca ttgactattt tgtcatttgt
     4381 ttcagagtaa ccgtttttcc agttgctccg ttctgcgcgt tcaggaaaat aaattcctgc
     4441 aatataagaa ccaaacatgg tttctccaga ataatattcc tcaaaattac cacgttgacc
     4501 catgtgtccg ttacctaaac taaaaatact ttctgaagca agctggctgt ccatatgaaa
     4561 accttcttct ataatacacc agggatcatg ttgcaaatac ttattcataa tattttgatt
     4621 ttaaaaataa actaaaaaaa tcaagacctt ttctttattg gaaaaattat ttttactgaa
     4681 aaattttaat catttaacat aatactcttg tagataaaaa acaaaagaat taaaaaatat
     4741 tagtatctgc aaatatattt tatttgtttg atttttacag taaaaaaaca acaatatttc
     4801 atcaagaaaa tcgtattttt tgaaaaagtt cacttcaaaa aaattgaaaa tacacactta
     4861 ttgttaacgc ctcgtcgcat ttcaaaataa aattagagaa gcaaaatcaa tacgtaaatc
     4921 caaattataa aactttttga aacaatttat ttttcaaaga actttcttta ctttgctttt
     4981 caatgtgtat atttgtcata ctttttaaaa agaaacttat tttattgttt ttttaatttt
     5041 caaaaaagtt ttccacctac ttttaaagtt tttttcattg attaataaaa gattatgaac
     5101 aactttatca ctaacagtac taaaaaacaa ctcaaaacac gtcttattga acttctttct
     5161 aaaagtgaag aactgaaatt tctggttgga ttttt
//
LOCUS       DAOTKS010000079         5135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_14353_length_5135_cov_2.991929, whole genome shotgun sequence.
ACCESSION   DAOTKS010000079 DAOTKS010000000
VERSION     DAOTKS010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5135
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14353_length_5135_cov_2.991929"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="P5236_02520"
     CDS             complement(<1..933)
                     /locus_tag="P5236_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63268.1"
                     /translation="MIVKNIYESKSDFVKFSPSEKIVYEMVKKLYQKETETTERIKEN
                     SLPQTTLFNCVHTITSKLKEEEYPEAFIDEIWDEIKHIRGKRNDPIKNRPAGDFWGDD
                     YDFHVNRWYEATVIFACVYVIMAIDCPEKVDCLETIKEKCAYNENSTPYFEYFEKSLQ
                     DWKKNQERFFESSEIQKDLKENSTNHYSENEWIANEKHVSWEFDNFRKTLVGKSTDEK
                     FRDCLEEYQREQNMPHPNSLYLKLLDREIQYYQRHIAETNSPTIQVNDIISVLQENKF
                     SPAKAVQILEIINYILYARQYPEDICGNIDKKINL"
     gene            complement(1685..2815)
                     /locus_tag="P5236_02525"
     CDS             complement(1685..2815)
                     /locus_tag="P5236_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRU63269.1"
                     /translation="MSTTKVTLRKRELPSGKITLYLDFYPPVRNPFTMKMTRREYLGI
                     YIAKNPKTSLEKSINAEKLKQAEAIRAQRELSLINEQYGFMDKTKQKMDFLAYFKNKL
                     TEHDEKWRIVYEHFYKFVHGKCTFAEITVDLCNGFRTYLLSAKQLNHNHKALSQNSAS
                     GYWSTFRGLLAIAYKEKYIFENINEYLYKIETLDSRRQFLTFEEVQALYNTPCRETAL
                     KNASLFSCLTGLRFSDILALQWENFETYSDGGLCIRLRTKKTNAEVLNPISQEAYELC
                     GKKSTGLVFKGLKKSMLQNILPEWLNDAGINKHITFHSFRHTFATLLVSNGSEIYTVS
                     KMLTHKNVSTTQIYADLIDEKKRQAAEMIKIKPNEKDVDKSE"
     gene            complement(2820..3713)
                     /locus_tag="P5236_02530"
     CDS             complement(2820..3713)
                     /locus_tag="P5236_02530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRU63270.1"
                     /translation="MERKFEFQKVCQYCGQIFTALNSTTKYCSKTCANRAGKEEKRLK
                     RLNEDSNHIKEKYRQNLLSKDLLSITQAAYLLGVSRPTIYKMIANNELFTYRFTERIV
                     RIKRTDLEQKQLPLSPVNTPISEIQKQKEKLISKKEALEMFGISDTWFYKKIKSADLS
                     PTFIDGKAFYSINMLKKLFIKQGQQNISEWYSLPEIMDKFKVSRQYIYDYTSDHKIPK
                     KRQGKGILISKYHWDKARGLDPLENENYYTVEQAIKKLGIGRSHLYDLIRSYKMPKIK
                     RGRNILIERESIDNLIKYKKL"
     gene            complement(3977..>5135)
                     /gene="mnmE"
                     /locus_tag="P5236_02535"
     CDS             complement(3977..>5135)
                     /gene="mnmE"
                     /locus_tag="P5236_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRU63271.1"
                     /translation="YTGEDVVEISCHGSIYIQQQILQCLIDKGCVLAKAGEFTQRAFL
                     NKKLDLSQAEAVADLIAATSAASHRIALTQMRGGISNELKKLRSQLLDFVSLIELELD
                     FSEEDVEFADRSKLLRLADEIEKLMRKLSNSFKIGNALKNGIPVAIIGETNVGKSTLL
                     NVLLNEEKAIVSDIHGTTRDAIEDSVNIEGITFRFIDTAGIRETKDEIETLGIERTFQ
                     KIEQASIILWLIDCTQLSERIESLTEKIAKKVEKKKFILVFNKIDKLTEEERNVVWEF
                     FEHYKGERIYISAKKQLNIEKLKKALVKAAQLPEIQPGDVVISNVRHYEALQNALKAI
                     QRVKQGLENGLSGDFISQDLRECIYFLGEITGEITNDEILGNIFSKFCIGK"
BASE COUNT         1559 a          802 c          821 g         1953 t
ORIGIN      
        1 caagtttatt tttttatcga tatttccaca aatatcttca ggatattgtc tggcatatag
       61 aatgtaattg attatttcca atatttgaac tgcctttgca ggagaaaatt tattttcttg
      121 cagtacggaa attatatcgt tgacttgaat ggtcgggctg tttgtttcag caatatgacg
      181 ttgataatat tgaatctcac gatccagtaa tttcaaatat agactgttgg ggtgtggcat
      241 attttgctcc cgttgatatt cttccaggca atctctaaac ttttcatctg ttgattttcc
      301 aaccaatgtt tttctaaaat tatcaaattc ccaggaaaca tgtttttcat tggctatcca
      361 ttcgttttca gaataatggt tagtagaatt ttcttttaaa tctttctgta tttcagaaga
      421 ttcaaaaaat ctttcctgat ttttcttcca atcctgaagt gatttctcaa aatattcaaa
      481 gtatggagta gagttttcat tataagcaca tttttcctta atggtttcca gacaatccac
      541 tttctcggga caatcgatag ccataatcac atacacacaa gcaaaaatta ccgttgcctc
      601 ataccatcgg ttgacatgaa aatcgtaatc atccccccaa aaatctcctg ctggtctatt
      661 ttttatagga tcattccgtt tccctctaat atgtttgatt tcatcccaaa tttcatcaat
      721 aaatgcttcc ggatattctt cctctttcaa ttttgaggta atggtgtgaa cacagttgaa
      781 aagcgtagtt tgtggaagag aattttcttt tattcgttct gttgtttctg tttctttttg
      841 ataaagcttt ttaaccattt cataaacaat tttttcactg ggactaaatt tgacaaaatc
      901 acttttcgac tcataaatgt tttttacgat catggattat ttttttaaag ttaatgttta
      961 atgtaattgt ttgaactaat agtaatttta tgtgtattaa aaagagcaaa atgcagtttt
     1021 ctaattcgtt gttagccgct gttatcgaaa attatttgaa gaaattggga ggttataaac
     1081 gggataggaa agtttttagg aaaatgatat ttgaagtaag gaagattcat atttacagta
     1141 aacgtcacag acaaaagtta ggttaaaagt ttttatcagt tgaataaaaa tggttttata
     1201 attggaatga aacaccaatt tttttgaaat ctaaattttc agctaaaaag tgaaagagcc
     1261 attgaatcca atagctcttc cgttagatat ttctatcaat aaaaattgat aaattattcc
     1321 tcaaaacagt gttctattct gttgggattt tcaaagacaa aaatagaaat aataaacaaa
     1381 actgaaaaaa gagagtagaa ttttgagtct gttttttaac ttatatactg aaattattga
     1441 tcccgatttt gccaaattgt caaattgcca ttttggagat ttggcaattt ggagatttgg
     1501 agatttggca atttggggat attaaaattg gcttgtgatt tcatttattc acattatttt
     1561 ctttaatatc aagatttaaa tcgagtttaa tggcattggc aatgggcact acaaaaattt
     1621 tattattctc tgtaatgaaa atttttctta tttgcgatta ttcattgtcc agcatatttg
     1681 tagattattc actcttatca acatcttttt catttggctt aattttaatc atttctgctg
     1741 cctgacgctt tttttcatca attaaatcgg cataaatttg tgttgtagaa acgtttttat
     1801 gagtcaacat tttgctaact gtgtaaattt cacttccgtt tgaaacgagc aaagtggcaa
     1861 aagtgtgtcg aaaagaatga aaggtgatat gtttattaat tcctgcatca ttgagccatt
     1921 ctggcaaaat attttgtaac atggattttt taagaccttt gaagaccaga cctgtagatt
     1981 tttttccaca taattcgtaa gcttcttgac tgattggatt caagacttca gcatttgttt
     2041 tctttgttct taatcttata caaagtccac cgtcagaata tgtttcaaaa ttttcccatt
     2101 gcaaagcaag tatatcactg aaacgtaacc cagtaagaca agaaaagagt gaagcatttt
     2161 tgagtgcagt ttcccgacat ggagtattgt aaagtgcttg tacctcttca aaagttaaga
     2221 attggcgacg ggaatctaaa gtttcgattt tgtaaaggta ttcattgata ttttcaaaaa
     2281 tatatttttc tttgtaagca atagccaaca ggccacgaaa tgttgaccaa taacctgaag
     2341 cagaattttg tgaaagagct ttgtggttgt gattaagttg cttggcactc aaaagataag
     2401 tgcgaaaacc attacacaaa tccactgtaa tttctgcaaa agtacatttg ccatgaacaa
     2461 atttgtaaaa atgttcgtaa acgattctcc atttttcatc atgctctgtt aatttgtttt
     2521 tgaaataagc caaaaaatcc attttttgtt tggtcttatc catgaaacca tattgttcat
     2581 taatcaatga caattctcgt tgagctcgta tagcttctgc ctgtttcaat ttttcggcat
     2641 taatgctctt ttcaagggat gttttcggat tttttgcaat ataaattccc agatattcgc
     2701 ggcgagtcat tttcatggta aatgggttac gaaccggagg ataaaaatca agataaagcg
     2761 taattttccc gcttggcaat tctcgtttgc gtaaggttac ttttgttgtt gacattgagt
     2821 tataattttt tgtattttat gagattatca atactttcac gttctatcaa tatgtttctt
     2881 cctcgtttaa tttttggcat tttgtatgaa cgaattaaat catataaatg acttcttccg
     2941 atacctaatt tttttattgc ctgttcgaca gtataataat tttcattttc gagcggatct
     3001 aaccctctgg ctttgtccca atgatatttt gaaattaata ttccctttcc ttgtcttttc
     3061 ttaggtattt tgtgatcact ggtatagtcg taaatatatt ggcgggaaac tttaaattta
     3121 tccataattt caggcaaaga ataccattct gatatatttt gttgtccttg ttttatgaaa
     3181 agttttttga gcatatttat tgaataaaaa gctttgccgt caatgaaagt gggagagagg
     3241 tctgccgatt tgattttctt gtagaaccaa gtatctgaaa ttccaaacat ttcaagggct
     3301 tcttttttac tgattaattt ttctttttgt ttttgtattt cagaaatggg tgtattgaca
     3361 ggtgaaagtg ggagttgctt ttgttccaag tctgtgcgtt taattcttac aattctttct
     3421 gtaaatcgat aagtaaataa ttcattattt gcaatcattt tgtaaatcgt tggacgtgaa
     3481 acgcctaaaa gatatgctgc ctgggttatg gaaagcaaat cttttgaaag caaattttga
     3541 cgatattttt ctttaatatg gttgctgtct tcatttaaac gttttaagcg tttttcttct
     3601 ttaccggcac ggtttgcaca agttttacta caatatttag tagtactatt cagggcagtg
     3661 aatatttgcc cgcaatattg acagactttc tgaaattcaa attttcgttc cattatttat
     3721 tttgctgata tttcgttatt attagagttt atttattgta aattggcgta aatttatgta
     3781 aattgacgta aaaatactgt atcatttagt ataagtattg tgagatttat atttatatgg
     3841 tacaaatgtg gtacaaagat atacaaaata tctgtaaaaa agaataaata aaggaagaaa
     3901 attattgaag gaataaatta taaataactg tatttcaata gtttatttat tcttgattga
     3961 ttgttgttag gagggactat tttccgatac aaaacttact aaaaatatta ccgaggattt
     4021 cgtcattagt aatttcacct gttatttcgc ccagaaaata aatgcattct cgtaaatctt
     4081 gtgaaataaa atcgcctgac agcccatttt cgaggccctg ttttactctt tggatagctt
     4141 ttaaagcatt ttgtaaagct tcgtaatgtc tcacgttgct gattaccaca tcaccaggct
     4201 gaatttcagg taattgagct gctttaacga gtgctttttt taatttttca atgtttaatt
     4261 gttttttagc agaaatataa attcgttcac ctttgtaatg ttcaaaaaac tcccaaacca
     4321 cattccgttc ttcttctgta agtttgtcta ttttattgaa taccagaata aattttttct
     4381 tttcaacttt tttagcaatt ttttcggtca atgattcgat acgttcactc aattgtgtgc
     4441 aatcaatgag ccataaaata atagaagctt gctcaatttt ctgaaaagtt cgttctatgc
     4501 caagcgtttc tatttcgtct tttgtttcac gtatgccggc tgtgtcaata aaacgaaagg
     4561 taattccttc aatgttcacc gaatcttcga ttgcatctcg agttgtgccg tgtatatcag
     4621 aaacaatggc tttttcttcg tttagtaaaa cattcaataa tgttgatttt cccacattgg
     4681 tttcacctat aatagccaca ggaattccat tttttaaagc gtttccaatt ttaaaggaat
     4741 ttgataattt tctcatcaat ttttcaattt cgtctgcaag tctcaataat tttgatcggt
     4801 cagcaaattc cacatcttct tctgaaaaat cgagttctaa ttcaatcaat gaaacaaaat
     4861 caagaagctg tgaacgaagt ttctttaatt cattggaaat gccacctctc atttgagtta
     4921 atgctattcg atgcgaagca gcagaagtgg cagcaattaa atcagcaact gcctcggctt
     4981 gagacaaatc caatttctta tttagaaaag ctctttgtgt aaattcgcca gcttttgcta
     5041 agacacaacc tttatcaatt aagcattgta aaatttgttg ctgaatataa atagatccat
     5101 gacaagaaat ttccacaaca tcttcacccg tgtaa
//
LOCUS       DAOTKS010000080         5013 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_14728_length_5013_cov_3.478419, whole genome shotgun sequence.
ACCESSION   DAOTKS010000080 DAOTKS010000000
VERSION     DAOTKS010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14728_length_5013_cov_3.478419"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="P5236_02540"
     CDS             complement(<1..623)
                     /locus_tag="P5236_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRU63272.1"
                     /translation="MKLLEGKVAIITGAARGIGKAIALKFASEGADIAFTDLIIDENA
                     LNTQKEIASFGVKVKGYASNAANFEETHAVVEEILKEFGHIDILVNNAGITKDGLIIR
                     MSEAQWDAVINVNLKSAFNFIHACAPIMMKQKAGCIVNMSSVVGVSGNAGQANYSASK
                     AGLIGLTKSVAKELGSRGIRANAVAPGFIETEMTHALSDEQRAKWVE"
     gene            complement(804..1331)
                     /locus_tag="P5236_02545"
     CDS             complement(804..1331)
                     /locus_tag="P5236_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HRU63273.1"
                     /translation="MENRIEIVKGDITKEEVDAIVNAANRSLLGGGGVDGAIHRAAGP
                     ELLEECKKMNGCETGEAKITKGYRLPAKYVIHTVGPVWNGGNYHERELLANCYKNSLK
                     LGQQYGIKTIAFPSISTGAYRFPIELASKIALNEIYQFLKNNTSIEKVKIVCFDNQTY
                     ETYQRNLEELMKNER"
     gene            complement(1380..2072)
                     /locus_tag="P5236_02550"
     CDS             complement(1380..2072)
                     /locus_tag="P5236_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HRU63274.1"
                     /translation="MLKCNFLTDFINLLYPNLCLICGESLMKNEEQLCSKCLYQIPKT
                     NYHLIKNNLIEKRFWGKVPIEQATSFFFFQKGSPFQKLLHQLKYHGNKELGEILGKYA
                     AADLILTDYFSAVDMIVPVPLHPKKYNQRGYNQSEWIGKGLSTILQKPQIIDNLVRIK
                     ESTTQTKKSVYERYENTEGIFSVKDENFFSNHHILLVDDVLTTGSTLEACAHALLTAE
                     NVQISVFTLAVA"
     gene            complement(2095..2460)
                     /locus_tag="P5236_02555"
     CDS             complement(2095..2460)
                     /locus_tag="P5236_02555"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HRU63275.1"
                     /translation="MKIKEFLKSFVYAAEGIKVMFACGRNMKVNLFTVFVVIVLGIIF
                     HISAFEWISCLLCFGLVISLEMINTSFENLVNLVSPDFHPLAKKAKDIAAGAVLVSAI
                     IAFIIGLIIFLPKIIDAIS"
     gene            complement(2564..3133)
                     /gene="efp"
                     /locus_tag="P5236_02560"
     CDS             complement(2564..3133)
                     /gene="efp"
                     /locus_tag="P5236_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRU63276.1"
                     /translation="MATTADIKNGMCINLDGQFYIITEFLHVKPGKGPAFVRTKMKNV
                     VTGRTFEKTFSVGTHIDEVRIERRKYQYLYQDDMGYYFMNNETFDQVCINKEMINGVD
                     FLKEGMICDVVVHAESETILYVDLPIQVTLTVTYTEPGLKGDTATNATKPATLETGAN
                     IRVPLFINTGDTIRVDTRDGSYVERVKTE"
     gene            complement(3308..>5013)
                     /locus_tag="P5236_02565"
     CDS             complement(3308..>5013)
                     /locus_tag="P5236_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRU63277.1"
                     /translation="GTEFIKIEVPTEWATLPDDEEIEDNAPEFIKKVVRPMNAQAGDD
                     LPVSVFKGREDGTWEPGTSKYEKRGVATMVPLWNPANCTQCNECAYVCPHATIRPFIL
                     DETEQAKATFETIKANGKQFVGMTYRIQVDVLDCMGCNNCADICPGTRGNKALTMVPI
                     ETQYDNQKNWDFCINEVKSKQHLVDVKMNVKNSQLATPLFEFSGACAGCGETPYVKLI
                     TQLFGDRELVANATGCSSIYSASAPSTPYTTNKNGQGPAWANSLFEDNAEFGLGMVFA
                     NEIMRNRLVKIMTEAINDNSCSNELKALFAEWLDNREDVEKTKELASKIVPFVNTCNG
                     KYCKEIAELSSFLVKRSQWIIGGDGWAYDIGFGGLDHAIASGKNFNILVLDTEVYSNT
                     GGQSSKATPMGAVAKFAASGKRIRKKDLGMMAMAYGYVYVAQIAMGANKAQTLRALRE
                     AEAYNGPSLIIAYSPCISHGIYAGMEKAQEEQARAVECGYWQLYRYNPMLEEEGQNPF
                     ILDSKEPQWDKFQDFLMGEIRYASLMKQYPEEAKELFLAAEKNAKWRYNNYKQMAERQ
                     F"
BASE COUNT         1620 a          962 c          695 g         1736 t
ORIGIN      
        1 ttttcaaccc attttgctct ttgttcgtca cttaaagcat gggtcatttc agtttcaata
       61 aaacctggtg ctactgcatt tgctcgaatg ccacgagaac caagttcttt tgctactgat
      121 ttggtaagtc caatcaaacc tgcttttgat gcagaataat tagcttgtcc agcatttcca
      181 gataccccaa caacagaact catattaaca atacaaccag ctttttgttt catcataatg
      241 ggtgcacaag catgaataaa attaaaagct gatttcagat ttacattgat aacggcatcc
      301 cattgtgctt cactcattcg tataattaaa ccatctttgg taattccagc attattcacc
      361 aatatatcaa tatgtccaaa ttctttgaga atttcttcca ctactgcatg agtttcctca
      421 aagtttgcag cattggaagc ataacccttt acttttacac caaaagaagc aatttctttt
      481 tgcgtattca aggcattttc gtcaatgatt aaatcagtaa aagcaatatc ggctccttca
      541 ctggcaaatt taagggcaat tgcctttcct attccacgtg cagcacctgt gataatcgct
      601 acttttcctt ctaataattt cataagttaa tttgttaatg aattaatttt taaaatttca
      661 gcacaaaagt aagattttat tgataaatga gaaaagtttt taatttataa atttctaaat
      721 ttctaaatat aaacacaatc tatttataat caataaaata aatgtcttga taaataatta
      781 caaagtagaa ttgagtaaaa attctacctc tcatttttca taagttcttc caaattacgt
      841 tgataagttt cataagtttg attatcaaaa caaacaattt ttactttttc aattgaagtg
      901 ttatttttta aaaattgata aatttcgttc aaagcaattt tgcttgccaa ttcaatgggg
      961 aatctataag ctcctgtact aatagagggg aatgcaattg ttttaatgcc atattgttgt
     1021 cctaatttta aactgttttt atagcaatta gctaataatt ctctttcgtg ataattgccg
     1081 ccattccaaa ctggaccaac ggtatgaatc acatatttag ctggcaagcg ataacctttt
     1141 gtaattttcg cttcacccgt ttcacaacca ttcatttttt tgcattcttc aagcaactct
     1201 ggaccggctg ctctatgaat agctccatct acacctcctc cacctaaaag acttcgattg
     1261 gctgcattga caatcgcatc aacttcttcc tttgtaatat cgccttttac aatttctatt
     1321 ctattttcca tattccatga tttataattt gtgaataatt tttcaaaata ttggtaaact
     1381 caagccactg ctaatgtaaa aacgcttatt tgaacatttt cggctgtcaa aagtgcatga
     1441 gcacaagcct ccaaagtaga acctgttgtt aaaacatcat caaccaataa aatatgatga
     1501 tttgaaaaaa aattttcatc tttcacagaa aaaatgcctt ctgtattttc ataacgctcg
     1561 taaactgatt ttttggtttg agtagtactt tctttaatac gtactaaatt atcaatgatt
     1621 tgtggttttt gaaggatagt tgataatcct ttaccaatcc attcactctg attataacca
     1681 cgttgattgt attttttagg atgaagtggt actggtacaa tcatatcgac agcagaaaaa
     1741 taatcggtca aaatcaaatc tgcagcagca tattttccta aaatttcacc taattctttg
     1801 ttgccatgat acttcaactg atgcaataat ttttgaaaag gacttccttt ttgaaaaaag
     1861 aagaaagaag tagcctgttc aattggaacc tttccccaaa aacgtttttc tataagatta
     1921 ttttttatga gatgataatt cgttttagga atctgataca aacattttga acataattgt
     1981 tcctcatttt tcattaaact ttctccacaa ataagacata ggttggggta taaaaggtta
     2041 ataaaatcag tcagaaaatt acattttaac attttacatt gttttttaaa agcttcaaga
     2101 aatggcatca ataattttcg gtaaaaatat gattaatcca ataataaaag caataattgc
     2161 tgaaaccaaa acagctcctg ctgcaatatc tttggctttt tttgctaacg gatgaaaatc
     2221 gggcgaaact aaattgacca aattttcaaa agaagtatta atcatttcca gactgattac
     2281 caaaccgaaa cataataggc aacttatcca ttcaaaagca ctgatatgaa aaattattcc
     2341 taaaacaata actacaaaaa cagtaaataa attaactttc atgttccttc cacaggcaaa
     2401 cattactttt ataccttcag cggcatagac aaagcttttt aaaaattctt ttattttcat
     2461 gacaattttt ttatctatgc acaaaaataa aaaaaatccg ataaacttaa ttatttatcg
     2521 gataaataga tcattcaaaa aaatcgtgag aaaatatttt taactattca gttttgactc
     2581 tttctacata agaaccatca cgagtatcaa ctctgattgt atcccctgta ttgataaaaa
     2641 gaggaacgcg aatattggca cctgtttcga gagtagctgg tttggtggca ttagtagcag
     2701 tatctccttt aagccctggc tctgtgtaag ttacagttaa cgttacctga ataggtaaat
     2761 ccacatataa aatagtttcc gattcagcat gtaccaccac atcacaaatc attccttctt
     2821 tcaaaaaatc tacgccattg atcatttctt tgtttatgca aacttgatcg aaagtttcat
     2881 tgttcataaa ataataaccc atatcatcct ggtaaagata ttgatactta cgtcgttcta
     2941 tacgtacttc atcaatgtga gttcctacac tgaaagtctt ttcaaaagtt ctgcccgtca
     3001 ctacattttt cattttcgta cgaacaaaag caggtccttt acccggtttt acatgaagaa
     3061 attctgtgat gatataaaat tgaccatcaa gattaataca cattccattt ttaatatctg
     3121 ctgttgtcgc catatcattg ttaaaaattt gaatttaatt acctgagaaa gatttgtttt
     3181 acaaaaataa taattttatt tatattttca aaataaattg attatcagaa ttatattatc
     3241 aaaaaagcta ttcaaatgaa tagccttttt gataactcaa agaattattt aagaataaat
     3301 tattatctta aaattgtctt tcagccattt gcttatagtt attataacgc cattttgcat
     3361 ttttttcggc tgcaaggaat aattctttag cttcttcagg atattgtttc atcaaagaag
     3421 cataacgaat ttcgcccatc agaaagtctt gaaatttatc ccattgtggt tcttttgaat
     3481 ccaaaataaa tggattttga ccttcttctt ccagcattgg attataacga tacaactgcc
     3541 agtaaccaca ttcaacagct cgtgcctgtt cttcttgggc tttttccatg cctgcataaa
     3601 taccgtgact aatacaagga gaataagcaa taattaatga tggtccatta taagcttcag
     3661 cttctcttaa agccctgagc gtttgagctt tatttgctcc cattgcaatc tgagctacgt
     3721 aaacataacc gtaagccata gccatcatgc ccaaatcttt tttacgaata cgtttgcccg
     3781 aagcggcaaa tttagcaact gctcccatcg gagtagcttt agaagattgt cctcctgtat
     3841 tagaataaac ttcagtatct aatactaaaa tattgaaatt ttttcctgaa gcaatggcgt
     3901 gatctaaacc tccaaaacct atatcataag cccaaccatc gcctccgata atccattgcg
     3961 aacgttttac taaaaaagaa cttaattcag ctatttcttt acagtatttt ccattacaag
     4021 tattaacgaa gggaacaatt ttagaggcta attcctttgt tttttcaaca tcttctcgat
     4081 tatccagcca ttcagcaaat aaagctttca attcattgga acagctatta tcatttattg
     4141 cttctgtcat tattttgacc aagcgatttc gcataatttc gttggcaaat accattccca
     4201 gtccaaattc agcattatct tcaaacaaag aatttgccca ggctggacct tgtccgtttt
     4261 tattggtcgt ataaggagta gaaggagctg aagcagaata aatagagctg caaccagtag
     4321 cgttggcaac caactcacgg tcaccaaaaa gctgagtaat taatttcaca taaggtgttt
     4381 caccacaacc agcacaagct cctgaaaatt caaacagtgg agtagctaat tgggaatttt
     4441 ttacattcat ttttacatct accaaatgtt gtttcgattt gacttcgttt atgcaaaaat
     4501 cccaattttt ctgattatca tattgcgttt caataggaac cattgtaagt gctttattac
     4561 ctcttgttcc tggacaaatg tctgcacaat tattacaccc catacaatca agtacatcca
     4621 cttgaatacg ataagtcatt cctacaaact gttttccatt agctttgatg gtttcaaaag
     4681 ttgcttttgc ttgttctgtt tcatctaaaa taaatggtct aatagtagca tgcggacaaa
     4741 cataagcaca ttcgttgcat tgcgtacaat ttgctggatt ccatagggga accattgttg
     4801 ctacacctcg tttttcatat ttactggtac ctggctccca cgtgccatct tctcgccctt
     4861 taaaaaccga tactggcaaa tcatcgcctg cctgagcatt catcggacga accacttttt
     4921 taatgaattc aggtgcatta tcttctatct cttcatcatc tggtaacgtt gcccattcag
     4981 ttggaacttc tattttaatg aattctgttc ctc
//
LOCUS       DAOTKS010000081         5007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_14751_length_5007_cov_4.206179, whole genome shotgun sequence.
ACCESSION   DAOTKS010000081 DAOTKS010000000
VERSION     DAOTKS010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14751_length_5007_cov_4.206179"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /gene="argG"
                     /locus_tag="P5236_02570"
     CDS             complement(<1..1157)
                     /gene="argG"
                     /locus_tag="P5236_02570"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRU63278.1"
                     /translation="MKEKVVLAFSGGLDTSFCVKYLSEEKGFDVYTAIANTGGFSEED
                     LKKIEEKAYQLGAVKHVTLDITREFYDKGIKYMVFGNVLRNGTYPISVSSERIFQAIA
                     IINYAKEIGANYVAHGSTGAGNDQVRFDLTFQVLAPEINIITPIRDLSLTREYEIDYL
                     KQHGYEADFKKMEYSINKGLWGTSIGGKETLKSEQTLPEEAYPSQVTEKEEKIISIEF
                     DKGEICGINGEKKTDKIALIQQLENIASKYGIGRDMHIGDTIIGIKGRVAFEAAAPIL
                     IINAHKMLEKHTLTKWQQYWKDQLGTWYGMFLHEALYLEPVMRDIEKFLESSQKNVSG
                     TVYIKLRPYCFTLVGVDSPFDLMKTELGEYGETNKGWTAEEVKGFIKMMANP"
     gene            complement(1214..1798)
                     /locus_tag="P5236_02575"
     CDS             complement(1214..1798)
                     /locus_tag="P5236_02575"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRU63279.1"
                     /translation="MEKDFNIDEITVQVADERFLGYVDTILKTIEDAAKVRGTGIARR
                     SPEYIAQKIKEGKAIIALQGEEFAGFCYIETWSDKKFVANSGLIVVEKFRGHGLAKRI
                     KQKAFELSRQRYPDAKIFGLTTGLAVMKINSELGYKPVTFSELTSDEAFWKGCESCVN
                     YDILQRTNRKYCLCTAMLYDPAKEENKNNQPTEK"
     gene            complement(1853..2323)
                     /locus_tag="P5236_02580"
     CDS             complement(1853..2323)
                     /locus_tag="P5236_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HRU63280.1"
                     /translation="MKNKNNRLKMISEILRTHVVGSQEELLNLLKEQHCEVTQATLSR
                     DLKQLKVAKTPLSNGGYKYVLPTYNRPLPSVSENSLENSPANGAILSLEFSGQLGVMK
                     TKPGYANAIAWDIDNRSSDYILGTIAGDDTILIIPREGVSREDITEIIKEIFSN"
     gene            complement(3678..3941)
                     /locus_tag="P5236_02585"
     CDS             complement(3678..3941)
                     /locus_tag="P5236_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63281.1"
                     /translation="MIVYILLSFRYRYEYYESMGELFRVMNAECLQQTLDERLWGLFL
                     ELLCEICEALEKDIDELFELIMNCSKTAELVSRMLVPSLQEIV"
     gene            complement(3938..4066)
                     /locus_tag="P5236_02590"
     CDS             complement(3938..4066)
                     /locus_tag="P5236_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63282.1"
                     /translation="METYQIRCLIETLFKETKQLLNLEGWQSNDFDAQNSRYHCKY"
     gene            complement(4515..4991)
                     /locus_tag="P5236_02595"
     CDS             complement(4515..4991)
                     /locus_tag="P5236_02595"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HRU63283.1"
                     /translation="MKRQLKIALVAHDNRKVDMVEWALYNAEMLSQHKLFCTGTTGKL
                     IKKAMAERGIEVDITCMHSGPLGGDAEIAALVVQKKIDLAVFLIDDLNPQPHEADIQM
                     LLRQCRIHNVPLACNRYSADLMITSTLWDDETYVPTEPKYVKFVREDFFNSDEDLD"
BASE COUNT         1637 a          898 c          650 g         1822 t
ORIGIN      
        1 ggatttgcca tcattttgat aaatcctttt acttcttcgg ctgtccaacc tttattggtt
       61 tctccatatt ctccaagttc agttttcatt aagtcgaaag gactgtcaac acctaccaga
      121 gtaaagcaat aaggtcgaag tttgatataa acggttccag aaacattttt ctgagaactt
      181 tccagaaatt tttcaatatc tcgcataaca ggttccagat aaagtgcttc gtgcagaaac
      241 ataccatacc acgtacccaa ttgatctttc cagtattgtt gccatttggt aagtgtgtgt
      301 ttttcaagca ttttatgagc gttaatgatt aatatcggag ctgcagcttc aaaagccact
      361 cgtcctttta tgccaataat ggtatcgcca atgtgcatat ctctaccaat tccatatttt
      421 gaagctatgt tttccaactg ttgaatcaag gcaattttat ctgtcttttt ttctccattg
      481 ataccgcaaa tttctccttt atcgaattca atagaaataa ttttttcttc tttttcagtt
      541 acttgcgaag gataagcttc ttctggaagt gtttgctctg atttcaatgt ttccttaccc
      601 ccaatactgg ttccccacaa acctttatta atggagtatt ccattttttt aaaatcagct
      661 tcgtagccat gttgctttaa ataatcaatt tcgtattcac gcgtgagaga taaatcacga
      721 atgggagtaa tgatatttat ttcaggagcc aacacttgaa atgttaaatc gaaacgaacc
      781 tgatcatttc ccgctccagt acttccatga gctacataat tagcaccaat ttctttcgca
      841 tagttgatga tggctattgc ttgaaaaatt ctttcggagc tgactgaaat aggataagtg
      901 ccattgcgaa gaacattacc gaaaaccatg tatttaatac ctttatcata aaattctctt
      961 gtaatgtcca atgttacgtg ttttactgct cctaattgat aagctttttc ttcaattttt
     1021 tttaaatctt cttctgaaaa gcctcctgta ttagctatgg cagtgtaaac atcaaatcct
     1081 ttttcttctg ataaatattt tacacaaaac gaagtatcca aacctccact gaatgctaaa
     1141 acaacttttt ctttcatttt aagttataat tagtttaaaa ttagtttttt atcgataaat
     1201 aacaactgat aaatcatttt tcagtcggct gattattttt gttctcttct tttgcaggat
     1261 cgtaaagcat agctgtacaa aggcaatatt tgcgattagt tctttggaga atatcataat
     1321 ttacacaact ttcacatcct ttccaaaaag cttcgtcact ggtaagttct gaaaatgtta
     1381 caggtttata acccagttca gaatttattt tcattacagc cagtccggta gtcaatccaa
     1441 aaatttttgc atcaggataa cgctgacgtg aaagttcgaa agccttctgt ttaattcgtt
     1501 ttgctaaacc gtgtccacga aatttctcca ccacaatcaa tcctgaattg gctacaaatt
     1561 ttttatcgct ccacgtttca atataacaaa atcctgcaaa ttcctctccc tgaagagcaa
     1621 taatagcttt cccttctttt attttttgtg caatatattc tggcgaacgg cgagctatgc
     1681 ctgtaccacg cactttagca gcatcttcaa ttgttttcag aatcgtatca acatatccta
     1741 aaaaacgttc atcagctact tgaaccgtaa tttcatcaat attaaaatct ttctccatct
     1801 tttttaaatc tattttaaaa tactttttaa agatttactt aaaatttgat tatcagttac
     1861 taaatatttc ctttataatt tctgttatgt cttctctgga tactccttca cgtggaataa
     1921 ttaaaatagt atcatcgcct gcaatggtac ctaaaatata atcagaagaa cgattgtcaa
     1981 tatcccacgc aatggcattt gcataaccag gctttgtctt cattacgcct aattgtcctg
     2041 aaaattcaag acttaaaata gctccattag ctggagaatt ttccaaagaa ttttctgaaa
     2101 cggaaggcaa aggtctatta taggtgggta atacgtactt atatccaccg tttgagagag
     2161 gagttttggc aacttttaat tgttttaaat ctctcgataa agttgcttga gttacctcgc
     2221 agtgctgttc ttttaaaaga tttagtaatt cttcctgact tcctacaaca tgtgtacgaa
     2281 ggatttcaga aatcattttt aaacggttat tcttgttttt cataagttgt tgtctatttt
     2341 atatcaaaaa tcataggaat ttgcccataa aactttttgt gtataatttt ttaatataat
     2401 tcatgtcgta atgaatattt attaaaaaat ctttgcaaaa ttactatatt tattcttaaa
     2461 aatgatatta aataattata aatattcttt ccttaaaaat tattatgact tttataacat
     2521 gtactaaaaa ataaaaatta atttttataa ttatttaatc aaatcttcct aaaaaataaa
     2581 ttaaaaatga atatttataa aaccattatg atatttcaaa aacataaata ctaataaaaa
     2641 tacttaccct caggaacatc aatgaggaat taaattcttt gcttcaaaat ttctaagtac
     2701 atgacaaaaa ttcttaactg tctaaaattt tctgacaatc aggcatttat atgagggact
     2761 ttgctccaaa tctcacttta ttttttctta aaaaatgttt cactttttta gttgtttcag
     2821 tgatttcagc ttcgcttcaa ttgttttgaa aaaagtaaaa aacgttttac tatttcgttt
     2881 gttttttgag atttttctct ctataacttt gtaccttctc tgtggaattc tatgaaaata
     2941 aatgacttat tatactgatg aacaaaagat gattctttta tcgttgtgtt gttaaacgaa
     3001 actataaata ctcgaaattc gcaaatccaa aactcgtcaa ttttcggctc gccgaacaaa
     3061 gtcaatccaa aataactttt taattactga ataccaatat gatacggcaa cgtttatttt
     3121 tgtcgtggac aaaaagttta ataaaaagac gctgcctttt cgaaatgcat tttattattc
     3181 gtccgacaat aaaattatta atttgaacat gggaaaaaat agatttttca atatggaata
     3241 tggaaaaaac aagttttaaa attgaaacct tatcatttta aaaaccttca taaccattaa
     3301 caaacaaata aatcaaacct tattacctta taaagagcag aataaaatac agatggaaag
     3361 aaagaaaata atcaacccaa gtaaataagg taatatggtt atgagtgagg taaaacttaa
     3421 gcgttatgaa aattgtctgt ttttgatgaa gttttaaaga caaaaaatga taaaaacaat
     3481 gaaaattttt acgttaaaaa ccaaaaatgg ttctggggaa gagtggattt ttccttattg
     3541 aatataagta aagaataatt ttttatttta ctaatgcttt ttcgtacttt gatttaatgg
     3601 tttgacaaat aaaaacaatg aagtacgatt taattatttt tatcctgatt atcaatatgt
     3661 taataattcc attcccgtca tactatttcc tggagtgaag gaacaagcat tcttgaaact
     3721 aattctgccg tcttagaaca attcatgatc aactcgaaca attcatcaat atccttctct
     3781 aatgcttcgc atatttcaca cagcaactcc aaaaataatc cccaaagacg ttcgtcaagc
     3841 gtctgctgca aacattctgc attcattacc ctgaaaagtt ctcccatcga ttcatagtac
     3901 tcatatcgat atcgaaaaga taacaaaatg tagactatca atacttacaa tggtatcggc
     3961 tattttgtgc atcgaaatca ttggattgcc atccttccag attcaacaat tgcttcgtct
     4021 ctttgaaaag tgtttctatt aagcatctaa tctgataggt ttccataata tctttcgtga
     4081 atgagttcgt tgattattct tcaaacgaaa caaaacatcc ttatttatcg ttataactcc
     4141 ttcttttaac aagacaggga agatcctatc gttttccacc ttgtgacatg attaaatcaa
     4201 cgccaacaat atcacttgaa aactgtaact actccttttc aaattcttga actttgaaaa
     4261 actttccgtc agtttcaacg ccttgaaacc tttcaaaata tttatactct ccaatttatt
     4321 ttcataaaaa tctgtttcaa tctcacaaaa tccttcttct aactttgtcc ggtagcatat
     4381 ttttattttt ttaactatac agttaatata tactactttt aatatatact actttttact
     4441 tttttatctt cttttttact ttattcccag cactttctga aatgcgaaac tttagttatt
     4501 ctattatttt tattttaatc taaatcttca tcagaattaa aaaaatcttc tcgcacaaat
     4561 ttaacatatt tcggttctgt aggcacataa gtttcatcgt cccacaaagt gctggtaatc
     4621 attaaatcag cactgtaacg attacaagcc agtggtacat tatgaattct acattgacga
     4681 agcaacattt gaatatctgc ttcgtgtggt tgaggattga gatcatcgat taaaaaaact
     4741 gccaaatcaa tttttttctg aacaacaaga gcagctattt cagcatcacc tcctaaaggt
     4801 cctgaatgca tacaagtaat gtcaacttca attcctcttt ctgccattgc tttttttatt
     4861 aatttacctg tagtacctgt acaaaataat ttgtgctgag ataacatttc agcattatat
     4921 aaagcccatt ctaccatgtc aacttttcga ttatcatgag ctacaagagc tatttttaat
     4981 tgtcttttca taaaaaatta atctata
//
LOCUS       DAOTKS010000082         4999 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_14768_length_4999_cov_4.849515, whole genome shotgun sequence.
ACCESSION   DAOTKS010000082 DAOTKS010000000
VERSION     DAOTKS010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4999
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14768_length_4999_cov_4.849515"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            297..770
                     /gene="greA"
                     /locus_tag="P5236_02600"
     CDS             297..770
                     /gene="greA"
                     /locus_tag="P5236_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRU63284.1"
                     /translation="MGNITYMTSEGYKKLVEELNELENVQRPAISKQIAEARDKGDLS
                     ENAEYDAAKEAQGMLEMKIALLKETIASARLIDESRINTNEVQILTRVKIKNTKTQQV
                     MSYMIVSESEANLKEGKLSVTTPIAKGLLGKKVGDNVAIPVPNGVMQFEILEISL"
     gene            783..1178
                     /locus_tag="P5236_02605"
     CDS             783..1178
                     /locus_tag="P5236_02605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HRU63285.1"
                     /translation="MATIFSKIASGEIPCYKVAEDENYFAFLDINPLTKGHTLVIPKK
                     EEDYLFQLDDPTYIGLMLFAKKIALAIQKAVPCKRIGMAVIGLEVPHAHIHLIPINNE
                     SDMNFKNPKLQLSSEEMTEIASAIRQFIQ"
     gene            complement(1231..3237)
                     /locus_tag="P5236_02610"
     CDS             complement(1231..3237)
                     /locus_tag="P5236_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HRU63286.1"
                     /translation="MEILPLVKLDPWLEPYAEAIYGRYEYFLNTEKKLTEGKQSLSDF
                     ANGYLYFGLHHVPDGWVFREWAPNATSIYLIGDFNNWQRRPEYQLKKLQNGIWEIKLP
                     EYAMTHTQLYKLFVEWEGGSGERIPAWTQRVVQDPQTKIFSAQVWHPYQPYVFKIKNF
                     KPQKDPLLIYECHIGMSSQEEKISTYDEFRRNVLPRIAKLGYNAIQLMAIQEHPYYGS
                     FGYHVSSFFAASSRFGTPEELKQLIDEAHEMGIAVIMDLVHSHAVKNEVEGLGRFDGT
                     PYQYFHGGPRREHPAWDSLCFDYGKQEVMHFLLSNCKFWLDVYHFDGFRFDGVTSMIY
                     LSHGLGEDFTSYDCYYNLNQDGDAICYLTLANKLIHQVNPRAVTIAEEVSGMPGLAAK
                     IEDGGMGFDYRMAMNIPDFWIKIIKEVKDEDWKVGQIFWEVTNRRPEEKTISYVECHD
                     QALVGDKTIIFRLIDADMYWHMKRDDTTLQVDRGVALHKMIRLVTATTINGGYLNFMG
                     NEFGHPEWIDFPRPGNGWSFKYARRQWDLVDNQDYLYYNLNEFDRAMIALIKSVPAFN
                     KLPIQELWIKEDDQVLAYQRGKFVFVFNFNGTKSFTDYGILADKGAYKITLNTDNSLF
                     GGFNLVDESIVHFTIPEPPHFSGKEWLKLYIPARTAFVLKKQEM"
     gene            complement(3285..3731)
                     /locus_tag="P5236_02615"
     CDS             complement(3285..3731)
                     /locus_tag="P5236_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HRU63287.1"
                     /translation="MILDNLENEKMYESVHPHFRKAFDFIKSTDLLHLPIGKIELDGS
                     KLYVSVVEIEGKSEEEAKMETHKNFIDIQVPVDGEEIMGWKALQSLKKISQPYDSEKD
                     IAFYSDKASNLIKVQPGEFVIFFPTDGHQPGIFEGKHKKIIVKVLV"
     gene            complement(3766..4449)
                     /locus_tag="P5236_02620"
     CDS             complement(3766..4449)
                     /locus_tag="P5236_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HRU63288.1"
                     /translation="MSIQPILDYLSELKNNNNRIWFNQNRDWYEEVRSEFLRISSALI
                     TEISKFDEEIKYVNLKDCLFRIYRDIRFSPDKSPYKTNFGVYIAAGGGRKSPRGGYYL
                     HLDPTGSFVSVGIWNPEPKVLKVLRKSVFENIDEFNEIRSNPEFSTYFSYFLEDGKLK
                     KIPAGFPKDFPYAEFLKLRHYIVDYKLDTSFFEDNHVVSRLAEIMKAGYPLNQFLNFT
                     VDELLLNSF"
     gene            4650..>4999
                     /locus_tag="P5236_02625"
     CDS             4650..>4999
                     /locus_tag="P5236_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRU63289.1"
                     /translation="MKEKILQLQQEINQLTASSLEEVENLRIKYLSKKGKISLLFDEF
                     RNVPPAEKREIGQLLNQLKESAQSKINELKESLIISADEQQNIDLTRNADPIQIGTRH
                     PLSIIRNEIINIFG"
BASE COUNT         1769 a          891 c          756 g         1583 t
ORIGIN      
        1 gggcaaatga ggtaactggt taccctatag gagagcctgc agatgtcaaa accatcaata
       61 ataattttgt aataacgttg ggtgtaagat tttaatagtt aaatagtcaa tttttaagaa
      121 gtcattttaa gaattaacta tttagaaaaa atctttaaaa gttttgttat atcaaatata
      181 ttgaatatat ttgtttttaa atacgtttat caaaaaacac tctctatata agagttccag
      241 caaagagttg gaattctttt tattttttat atatcaaaaa cctaaaataa gtaattatgg
      301 gtaacataac ttatatgacc tccgaaggtt ataaaaaact tgttgaagaa ttaaatgaat
      361 tagaaaatgt tcaacgccct gctatttcca agcaaattgc tgaagcaagg gataaaggtg
      421 atttatctga aaatgccgaa tatgatgcag caaaagaggc tcagggaatg cttgaaatga
      481 aaatagcttt gttgaaagaa accatagcat cagcacgttt aattgatgaa tcaagaatta
      541 ataccaatga agttcagatt ttgactcgcg ttaaaattaa gaatacaaaa actcaacagg
      601 tcatgtctta tatgattgta tcagagagtg aggcaaactt aaaagaaggc aagttatccg
      661 taacaacacc tattgcaaaa ggactgttag gtaaaaaagt gggtgataat gttgcaattc
      721 ctgttcctaa tggagtaatg caatttgaga ttcttgaaat ttcactttaa aaaatacaaa
      781 cgatggcaac gatattcagt aaaattgcaa gtggcgaaat accttgttat aaggtagccg
      841 aagatgaaaa ttattttgct tttctggata ttaatccact tacaaaaggt catacattgg
      901 ttatacccaa aaaagaggaa gattatcttt tccagcttga cgacccgact tatattggat
      961 taatgctttt tgcaaagaaa attgctttag ctattcaaaa agccgttcct tgtaaaagga
     1021 taggcatggc tgtaattggt ttggaagtcc ctcatgctca tattcatctt attcctatca
     1081 ataatgaatc ggatatgaat tttaaaaatc caaagttaca actcagttct gaagaaatga
     1141 cagaaattgc gagtgctatt cgccaattta ttcaataaaa atgaaaagag tcgtattcaa
     1201 aattttttac gactctttaa aattactaaa ttacatttct tgctttttca gcacaaaagc
     1261 agttcgagct ggaatgtaaa gcttcaacca ttcttttccg ctaaaatgtg gtggctctgg
     1321 aatggtaaaa tgaacaatac tttcatctac cagattaaat ccaccaaaaa gagaattgtc
     1381 tgtgtttaag gttattttat aagcaccttt gtctgctaaa ataccataat cagtaaaaga
     1441 tttagtgcca ttgaaattaa atacaaaaac aaatttaccg cgttggtaag ccaacacttg
     1501 atcatcttcc ttaatccaca actcttgaat aggtaattta ttgaaagctg gcacagattt
     1561 aataagggca atcatagcac gatcaaattc attcaaatta taatataaat aatcctgatt
     1621 atctactaaa tcccattgac gacgagcata cttgaatgac cacccattac cagggcgagg
     1681 aaagtcaatc cattcaggat gtccaaactc atttcccata aaattcaaat agccgccatt
     1741 gattgttgtg gctgtgacta aacgaatcat tttgtgtaaa gccactccac gatcaacttg
     1801 taaagtagta tcatcacgtt tcatgtgcca atacatatct gcatcaatca atctgaaaat
     1861 aatagtttta tcaccaacca acgcttgatc atgacattct acataactaa tggttttttc
     1921 ttctggtctg cgattggtta cttcccaaaa aatttgtcct actttccaat cttcatcttt
     1981 tacttctttg attattttta tccagaaatc aggtatgttc attgccattc gataatcgaa
     2041 acccatccca ccatcttcaa tttttgctgc caatccaggc atgccactca cctcttcagc
     2101 gatggtcact gcacgtggat ttacttgatg aattagttta ttggcaagcg taagatagca
     2161 aatggcatca ccatcctgat tcaaattgta ataacaatcg taacttgtga aatcttctcc
     2221 taaaccgtga cttagataaa tcatagaagt gaccccatca aagcgaaagc catcaaaatg
     2281 atatacatct agccaaaatt tacaattcga taaaagaaaa tgcatcacct cctgttttcc
     2341 gtaatcaaaa cataacgaat cccacgcagg atgttctctg cggggtccac catgaaaata
     2401 ttgataaggc gttccgtcaa aacgacctaa accttctact tcatttttaa ctgcatgaga
     2461 atgaaccaga tccatgatta cggctattcc catttcgtga gcttcgtcaa tgagttgttt
     2521 taactcttcg ggcgtaccaa agcgagaaga agcggcaaaa aaactggata catgatatcc
     2581 aaaagagcca taataagggt gttcctgaat agccattaat tgaatggcat tataacctaa
     2641 ttttgctata cgaggtaaaa catttcgcct aaattcatcg taagtagaaa ttttttcctc
     2701 ctggcttgac ataccaatat gacattcata aataagaaga ggatcttttt gaggcttaaa
     2761 attcttaatc ttaaaaacat aaggttgata aggatgccac acttgagcac tgaaaatttt
     2821 tgtctgtgga tcttgtacta ctctttgagt ccaagcagga atacgttcac cacttccacc
     2881 ttcccattcc acaaaaagtt tataaagttg ggtatgagtc attgcatatt cgggtaattt
     2941 tatttcccaa ataccatttt gtaacttttt taactgatat tcaggacgtc gctgccaatt
     3001 gttaaaatca ccaataagat aaatggacgt ggcattgggt gcccattcac gaaaaaccca
     3061 gccatcagga acatgatgaa gtccaaaata caaatatcca tttgcaaaat cagataagct
     3121 ttgttttcct tcagttaatt ttttttctgt attcaagaaa tattcatacc gtccataaat
     3181 agcttctgca taaggttcca accagggatc caacttaaca agaggtaaaa tttccatatc
     3241 ttaaaattct tgatctctta acactaaatt tcctatcaaa agttttaaac caaaacctta
     3301 acgatgattt tcttgtgctt tccttcaaaa ataccaggct gatgaccatc tgttgggaag
     3361 aaaataacaa attcacctgg ttgcacttta attaaatttg aagctttgtc gctgtagaaa
     3421 gcaatatctt tttcagaatc gtaaggctga gaaatttttt tcaaactttg taatgctttc
     3481 caacccatta tttcttcccc atccacagga acttgaatat caatgaaatt tttatgcgtt
     3541 tccatttttg cttcttcttc acttttacct tctatttcta caacgcttac gtacaactta
     3601 ctcccatcca attcaatctt accgatagga agatgaagta aatcagtgct ttttatgaaa
     3661 tcaaaagctt ttctaaaatg aggatgtact gactcgtaca ttttttcatt ttctaaatta
     3721 tctaaaatca taaataaaag aattaaatgt ttattttaat gacgtttaaa aactatttaa
     3781 caacagctca tccactgtaa aattcaagaa ttgatttaaa ggataaccag ctttcattat
     3841 ttcagctaag cgagacacca catgattatc ttcaaaaaat gaggtatcaa gcttataatc
     3901 gactatgtaa tgtcttaatt tcaaaaattc agcatacgga aaatctttag ggaaaccagc
     3961 aggaattttt ttaagtttcc catcttccaa aaaataagaa aagtaagtgg aaaattcggg
     4021 attggaacga atttcattaa attcatcaat gttttcaaaa acacttttac gcaatacctt
     4081 caaaactttg ggctcaggat tccatatacc aaccgaaaca aaagaaccgg ttggatcaag
     4141 atgtaaatag tagccacctc gaggactttt gcgaccacct cctgcagcaa tataaactcc
     4201 gaaatttgtt ttatagggac ttttatcggg agaaaaacgg atatcacgat aaattctaaa
     4261 aagacaatct tttaaattaa cgtacttaat ctcttcatca aacttggaaa tttctgtaat
     4321 aagagcagaa cttatcctta aaaactcaga cctaacttct tcataccaat ctctgttttg
     4381 attaaaccat atacgattgt tgttgttttt caattcactt aaatagtcaa gtataggttg
     4441 aatagacatg ttttccccct ttttaaaaac acaaagatac tgaaaattaa agaaaattca
     4501 ttattttcct taatttttta aatttgttat tttcgaagaa gataacgcta tttttcctga
     4561 aaataattac ctttgcaagt tcaaattagt aatgaatgat tttaatttct tcaggaaatt
     4621 tttatctggt agaataaaag gacattaaaa tgaaagagaa aattttgcag ttacaacaag
     4681 aaattaatca attaactgcc tcatcgttag aagaagtaga aaatttacgg attaaatatt
     4741 tgagtaaaaa aggaaaaata tccttgcttt ttgatgaatt cagaaatgtt cctccagcag
     4801 aaaaacgaga aatcggacaa ttactaaacc aattaaaaga aagtgctcaa agtaagatca
     4861 atgagctgaa agaaagttta atcatttcag cagatgaaca acaaaatatc gacttaacaa
     4921 gaaatgccga ccccattcaa attgggacac gtcatccact ttctattatc agaaatgaaa
     4981 ttatcaatat ttttggtca
//
LOCUS       DAOTKS010000083         4980 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_14832_length_4980_cov_5.004264, whole genome shotgun sequence.
ACCESSION   DAOTKS010000083 DAOTKS010000000
VERSION     DAOTKS010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14832_length_4980_cov_5.004264"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="P5236_02630"
     CDS             complement(<1..228)
                     /locus_tag="P5236_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HRU63290.1"
                     /translation="MDAGSYAKDIVDTVSKNCDLFYIRANKSESMTAQISQISEWKKV
                     EINYKTYEVASIPFKQFFEERNYRLVVMREES"
     gene            complement(289..450)
                     /locus_tag="P5236_02635"
     CDS             complement(289..450)
                     /locus_tag="P5236_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63291.1"
                     /translation="MDYDNQIIEHEKWDAKRTYKHTTGYFAGIGSIGNRIVYVENRDG
                     NANVKTAQV"
     gene            complement(620..868)
                     /locus_tag="P5236_02640"
     CDS             complement(620..868)
                     /locus_tag="P5236_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63292.1"
                     /translation="MKLQKVSQSVSSFAGIFFVHKEFNKCGLLDLIDNCLERRNYSGY
                     QHGELFRTWFEIFFCGGEVAEDVQEHLRSTLENIPEAG"
     gene            complement(1091..1822)
                     /locus_tag="P5236_02645"
     CDS             complement(1091..1822)
                     /locus_tag="P5236_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008776358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRU63293.1"
                     /translation="MKKLLLIIGIMTATNLFSKTPAFESDVFETSMGKLTITFIGHGS
                     LMFEVNQTVIHVDPVGQFGDYSLLSQADLILITHDHYDHLDLSAIKKIAKSDTKIIIP
                     SVCKKAVPEGFIMQNGASTTFNNISIEAVPAYNLKHKNDKGEFFHPKGVGNGYVLTFG
                     NFKVYVAGDTENTPEMKSLKNIDVAFLPMNLPFTMTPEMVADAARSFKPKILYPYHYG
                     NTDPNQLVKLLKNEPNIEVRIRKLR"
     gene            complement(1882..2526)
                     /locus_tag="P5236_02650"
     CDS             complement(1882..2526)
                     /locus_tag="P5236_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63294.1"
                     /translation="MLFNISLLEWIGYLASIIVAISLTMSSIKKLRWLNLCGSAVFSF
                     YGFAIGALPVGLLNSFMVFVNVYYLIKMNSSVESFKTLQVPVNDPYFNYFIDFHKKEI
                     QEIFPNFDFSLLQNEGNNCSLSLLILRNAIAAGVFYGTIENKTLYVHIDFVTAEYRDL
                     KPGEYIYKKNVQQLKDFGIQRIISKSDYTKHQKYLLKMGFVSQINNPSIFVKEI"
     gene            complement(2644..4095)
                     /gene="rodA"
                     /locus_tag="P5236_02655"
     CDS             complement(2644..4095)
                     /gene="rodA"
                     /locus_tag="P5236_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRU63295.1"
                     /translation="MVRSSIKYALDWRTIFYYVILVIMGWLSVYAASFDFDHPGILDF
                     SQRSGKQFIWILSAFGLAGIILLIDSRIYNYFSYLIYGLTLLLLVVTIFIAPNIKGSR
                     SWIVIGPVNFQPAELAKMATSLALAKFMSGYNYKIKTWKDLIPLLVIIGIPLVLILLQ
                     KETGSALVFLSFFLVFYREGMKGIVLLLGIFAIVLFVLVIRFSMVPIQGDKGTLGIIL
                     AMGLILLVQFVYSFFIKKNKKEALFFLAGIVAVFFISYLLSLWIDINYNAVAFFTVIA
                     SCIYWAIVEIIHKHKQYLFLAIVSLCAALFSASSDYLFENVLEPHQKVRIKVLLNMES
                     DPLGAGYNINQAKIAIGSGGFTGKGFLNGTQTKLQYVPEQDTDFIFCTVGEEGGFLGS
                     VFVLIIYWLFLMRLITIAERQRDTFNRVYGYCVVSIFFFHLIVNIGMVLGIMPIIGIP
                     LPFFSYGGSSLWGFTILLFILLRLDASRIEHVR"
     gene            complement(4082..>4980)
                     /locus_tag="P5236_02660"
     CDS             complement(4082..>4980)
                     /locus_tag="P5236_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRU63296.1"
                     /translation="QYSPGSTFKTIQALVCQQMGGIDTNTLFPCNGPATSPIKCTHFH
                     GSPVNLLKGIEQSCNPYFWGAFHTTLELGGYGEKNVNFKKNYNTWRKHVLSFGFSQKF
                     TDGDIYQQSSGNIPAEKYYNHYFGETGWRAITIRSLAIGQGEILVTPLQLANAMAIIA
                     NKGYYITPHLNKSDSMLVRTHFTTVDKKYFNVVDEGMWRVCEYGTARHYKIPNMAMCG
                     KTGTVQNPFGKDHSIFVGYAPRENPKIAIAVVVENAGFGATWASPIANLMIEQYLFGK
                     INRVDLFNRISNSYFISSYGQE"
BASE COUNT         1706 a          919 c          753 g         1602 t
ORIGIN      
        1 cgattcttcc cgcataacca ccaatcgata attgcgctct tcaaaaaact gcttaaaggg
       61 aatggatgct acttcatatg ttttgtaatt gatctctact tttttccatt cgcttatttg
      121 gcttatctgt gccgtcatgc tttccgattt gttggcccga atatagaaaa gatcgcaatt
      181 ctttgaaacc gtgtcgacga tatcttttgc atatgaaccg gcatccatcc gtgaacgatt
      241 gatcgttatt ccgttatccc gcagttggcc gaacattctt gcgagcgttt acacctgtgc
      301 tgtcttaacg ttggcattgc catcccgatt ttctacataa acgatcctgt tgcctatcga
      361 gccaatgcct gcaaaatatc ctgttgtatg tttgtaggtg cgcttggcat cccatttttc
      421 gtgctctatg atttgattgt cgtaatccaa atcgtatttc ttaccctttt caagctctcc
      481 ggtcaatagc agggatttga tttcaaatca ttcatctttt cattgatgtt gaaccggtac
      541 tctttcccgc tcgaagaggt caccatcgta tcttctgttg ccaacgcttt cagttcccga
      601 agcagcgtat ccggagaggc taccctgctt cgggtatgtt ttccaatgtc gatcgcaaat
      661 gttcctgaac gtcttctgct acttctcctc cacaaaagaa tatctcaaac cacgtcctga
      721 acaactctcc gtgctgataa ccggaataat tccttctctc caaacaattg tctatgagat
      781 ctaacagacc gcatttgtta aattctttat gaacgaaaaa aattcctgca aatgaactca
      841 ccgattgcga aactttttgt aatttcatct tctgtaagat ttactttgtt ttttgtttac
      901 actcataaag atatgtgaaa tcttacactt ggcaaagcgt ttggtatttt gttttttatc
      961 aacgctttgc ttctttatat ttaaaattta ggtgcggaat taaggtaata gtaaagttgt
     1021 taaaaaacga atggctttaa attaatcagc cattcgttaa agtaggtgtt aacattctga
     1081 aacaaagtgt ttatcttaat tttcgaatcc tcacttcaat attgggctca tttttcaata
     1141 attttaccag ttgattggga tcggtattcc cgtaatgata aggatacaaa attttgggtt
     1201 taaaacttcg tgcagcgtca gctaccattt cgggcgtcat agtaaaaggc aaattcattg
     1261 gcaaaaaggc aacatcaata tttttaagtg atttcatttc aggagtattt tccgtgtcac
     1321 cagctacata aactttaaaa tttccaaacg ttaatacata tccgttacct acccctttcg
     1381 gatggaaaaa ttcacctttg tcatttttat gtttgaggtt ataagcaggt acagcttcaa
     1441 tactaatatt attgaaagta gtagaagctc cattttgcat tataaatcct tctggtacag
     1501 cttttttaca aactgatgga ataataattt tcgtgtcgct ttttgctatt tttttaatag
     1561 cagaaaggtc taaatgatca taatgatcat gcgtgatgag aatcaaatca gcttgagaaa
     1621 gaagtgaata atctccaaac tgtccgacag gatccacatg aataactgtt tgatttactt
     1681 caaacataag gcttccatga ccaataaaag taatagtcaa tttacccata gaagtttcaa
     1741 aaacatcgct ttcaaaagcc ggtgttttgc taaataaatt tgttgcagtc ataattccaa
     1801 taattaataa taattttttc ataacaatta gagctttaat atagttaaaa aaatttttct
     1861 aacaaaacaa aaatctttat tttaaatttc tttcacaaaa atagaaggat tatttatttg
     1921 agaaacaaac cccattttca ataaatattt ttgatgtttg gtataatctg attttgatat
     1981 aatccgttgg attccaaagt ctttcaactg ttgaacattt tttttataaa tatattcacc
     2041 tggttttaaa tctctatatt cagcagttac aaaatcaatg tgaacgtaaa gagttttgtt
     2101 ttcaattgtt ccataaaaaa cacctgctgc tatggcgttg cgaagtatta ataaggaaag
     2161 agaacaattg ttcccttcat tttgaagcaa actaaaatca aaatttggaa aaatttcttg
     2221 aatttctttt ttatgaaagt caataaagta attaaaataa ggatcattaa caggaacttg
     2281 taacgtttta aatgattcca cagaagaatt cattttaatg agataataaa catttacaaa
     2341 aaccatgaat gaattaagca agcctactgg taaagcacca atggcaaatc catagaaaga
     2401 aaatactgct gaaccacata aattcaacca gcgaagtttc tttatagaac tcattgtaag
     2461 cgaaatagct acaataatag aagctaaata acctatccat tcgagtaaag aaatattgaa
     2521 taacatattt ttctaaatga ttaactgagt tgaatttttg atttatcgac gactaaattt
     2581 ttccctttac aaaaataaac ttttaaagtc agaaatggtt cagaaatttt gtaaaaaagg
     2641 agattaacgt acatgttcta tccttgaagc atctaatcga agcaaaataa aaagcaaaat
     2701 agtaaacccc cataaggagg atccaccata actaaaaaaa ggcaaaggta ttcctataat
     2761 aggcattatt cctaatacca ttccaatatt gactattaaa tgaaaaaaga aaatactgac
     2821 cacacaataa ccatagactc gattaaaagt atcacgttga cgttcagcaa tggtaattaa
     2881 tcgcattaaa aagagccaat aaataattaa tacaaaaaca gaaccaagaa accccccttc
     2941 ttctccaacg gtacagaaaa taaagtccgt atcttgttcg ggaacatatt gtaatttagt
     3001 ttgtgtcccg tttaaaaaac cttttcctgt aaaacctccc gaaccaatag caatttttgc
     3061 ttgattaata ttatatccgg ctccaagtgg atcactttcc atattcaaca aaaccttgat
     3121 gcgaactttt tgatgaggtt ccaatacatt ttcaaataaa taatcggaag aggcactaaa
     3181 taaagcagca caaagtgaaa caattgctaa aaaaagatat tgtttatgtt tatgaattat
     3241 ttcaacgata gcccaataaa tacaggaagc aattaccgta aaaaatgcga cagcattgta
     3301 gtttatatct atccataaac taagcagata agatataaaa aaaactgcta caatgcctgc
     3361 caagaaaaat aaagcctctt ttttattttt tttaatgaaa aaagaataaa caaattgaac
     3421 caacaaaatc aatcccatcg ctaaaataat tcccagcgta cctttatcac cttgaatagg
     3481 caccatgcta aagcgaatga ctaaaacaaa taaaacaata gcaaaaattc caagcaacaa
     3541 gacaattcct ttcattcctt cacgataaaa aacaaggaaa aaagaaagaa aaactaaggc
     3601 agaaccggtt tctttttgta ataaaataag caccaatgga attccaataa taactaaaag
     3661 aggtattaaa tctttccatg ttttgatttt atagttatag ccactcataa atttggctaa
     3721 cgccagcgag gtagccattt tagctaattc agcaggttga aaattgacag gacctatcac
     3781 tatccacgaa cgagaacctt tgatattagg agcaataaaa attgttacaa ctaataataa
     3841 taaagtaagt ccataaatta ggtagctgaa ataattatat attctcgaat caataagcaa
     3901 aataattcca gccagaccaa aagcagagag aatccagata aattgttttc ctgagcgttg
     3961 gctaaaatcg agaatgccag gatggtcaaa gtcaaagctt gccgcataga cacttaacca
     4021 acccattatt accaatatca cataatagaa tatggttcgc caatccaaag catatttaat
     4081 actactcctg accatacgaa gatataaagt atgaatttga aattctgttg aataaatcca
     4141 ctctatttat tttcccaaac aaatattgtt caatcattaa atttgcaatc ggagatgccc
     4201 acgtagctcc aaatcctgca ttttcaacaa ccacagcaat agcgattttt ggattttctc
     4261 gcggggcata acctacaaaa attgaatgat ctttcccaaa aggattttgt actgtccctg
     4321 ttttgccaca cattgccata ttcgggattt tataatgtcg tgctgtccca tattcacaaa
     4381 ctctccacat tccttcatct accacattaa aatatttttt atctactgtt gtaaaatggg
     4441 tccttactaa catactgtct gatttgttga gatgaggtgt tatataatag cctttattgg
     4501 ctattattgc catcgcattt gccagttgca atggtgtgac cagtatttca ccttgtccaa
     4561 tggctaatga acggatagta attgctctcc accctgtttc accaaaataa tggttataat
     4621 atttttcagc aggaatattt cctgatgatt gctgataaat atcaccatcg gtaaattttt
     4681 gactaaatcc gaaactcaaa acatgttttc gccaagtatt ataatttttt ttgaaattga
     4741 catttttttc tccataacct cctaattcaa gcgttgtgtg aaaagctccc cagaaataag
     4801 gattacaact ttgttcaata ccttttaata agtttactgg tgaaccatga aaatgggtac
     4861 atttaatagg ggaggtggct ggtccgttac aaggaaataa agtattggtg tcaattccac
     4921 ccatttgttg acaaaccagt gcttgaatag ttttaaaagt tgaacctggt gaatattgtg
//
LOCUS       DAOTKS010000084         4913 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_15066_length_4913_cov_4.576986, whole genome shotgun sequence.
ACCESSION   DAOTKS010000084 DAOTKS010000000
VERSION     DAOTKS010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15066_length_4913_cov_4.576986"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2162)
                     /locus_tag="P5236_02665"
     CDS             complement(<1..2162)
                     /locus_tag="P5236_02665"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HRU63297.1"
                     /translation="MRNNKIFIPYFIVYLMLMFSFPFTSLSAQETLVVGQIVNQVNKQ
                     PIANAKIFFKNTSKIAVSNEEGYFLIRNSGKENVLEFSAMGYKNKEIKIKPGESIGIL
                     VEMEPEAVTINNSFTSKEKEAAIPIISQIRNHLEDNDETNSPDYKVIAIEQNLLILNK
                     IHKQNIDPQIYHYLQNASVTDSFLVVPMYMNEVKYQYTSKGKKEISKTNFSSPQTEEM
                     IISQVLGEINPSLNFYKPTVTFFGQKYISPLSEKAEFYYHFYLTDSILSDTGKLYEIH
                     FASKNQRHFAFEGKFQVDSATWAITYIEASTSKLDKSFIKNLTIFQKFEKNQENKWCR
                     SKEKILITFKDNNLVKKITSEFPLFFNRTTTYQQNTTVAYKNNFAQSGYSTEVLNDKL
                     NQFQNTKLMKLTNWTSDAIFSAYLSVGKIDIGKIQFLARTTGLEGWRVNLPLRTNEKL
                     CKNFTVGGYLGYGFGNQEVKYSTFAQYRLPGETKRVLGINYTNDNRRIDYNYNNFLYL
                     ENPLNSGDEDIASTIFSFRKAQRLNERKELTFSFSNDWNTNVESSIYFRTIQQFSNEF
                     IPFTRDNVSYSSTHQQSIIFSTRFSFDEKTFEDHLQRIYMYNHKPIIYTILEGGKYKV
                     GNQVNNYGKLVGIIKQNTQFNWAQLNYWLEGGWLIGKVPYSLLAFQPGCEGGYGFYRF
                     SMLDYMQYAADKYFQFHGELIFNGRVWQKIPVIKYLTL"
     gene            complement(2152..3078)
                     /gene="xerD"
                     /locus_tag="P5236_02670"
     CDS             complement(2152..3078)
                     /gene="xerD"
                     /locus_tag="P5236_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HRU63298.1"
                     /translation="MLLILDEYHYYLKLEKGLSDNTIDAYERDLSKLSQYLSEAHIPL
                     EQATTEHLRDFLIEIRDSGIHPRSQARIISGITSFYHFLIYKNIITEDPTELLESPKI
                     GLRLPEVLSVSEINAMVAAIDLSKPEGHRNKAIIEVLYGSGIRVSELIGLQISKMNLK
                     EGYMLVEGKGSKQRLVPLSPTSIEQIEMWKIDRNKLPIKKGNEDYLFLNRRGTKMTRA
                     MIFTIVKDLAAAAGIKKKVSPHTFRHSFATHLLENGANLRAIQQLLGHESITTTEIYT
                     HIDIHYLRETILNFHPLNQHHPKMTNNAKNEE"
     gene            complement(3318..4085)
                     /locus_tag="P5236_02675"
     CDS             complement(3318..4085)
                     /locus_tag="P5236_02675"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HRU63299.1"
                     /translation="MKKFLVIIQLVILCLFPIYSQKKGLINTGGGSSMSVVLNSVGLA
                     YCFGDIGGSYQEQFLNGINDWDISNTRYLVSLGLRQSFKNDLGYKINVLYGSFVGDDA
                     NSRNEMRDYSYKNKVVGFSLQAEYVILGGFDPYKLNLHTLYIYLGGGILNSDSKLWRK
                     GLSISETPSDRPYDKVKLTDTTPIIPVGIGYQYHLTSNFSIGAELGWIYSFSDYLDGI
                     ITQYSKRKDTLANLNITFTYKFKDTLTLPCHCLSYEK"
     gene            complement(4082..4816)
                     /locus_tag="P5236_02680"
     CDS             complement(4082..4816)
                     /locus_tag="P5236_02680"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HRU63300.1"
                     /translation="MKKFLVIIQLVILCLFPIYSQKKGLISTGGSSMSVVLNSVSLAY
                     CFGDVGGSYQEQFLNGINDWDISNTRYLVSLGLRQSFKNDLGYKINVLYGSFVGDDAN
                     SRNEMRDYSYKNKVVGFSLQAEYVILGGFDPYKLNLHTLYIYLGGGILNSDSKLWRKG
                     LSISETPSDRPYDKVKLTDTTPIIPVGIGYQYHLTSNFSIGAELGWIYSFSDYLDGII
                     TQYSKRKDTLANLNITFTYKFKDLML"
BASE COUNT         1703 a          797 c          697 g         1716 t
ORIGIN      
        1 ttcaacgtga gatatttaat cacagggatt ttttgccaca ctcgaccatt aaaaatcagt
       61 tcaccatgaa attgaaaata tttatcagca gcatattgca tataatccaa catgctaaat
      121 cgataaaagc catagccacc ttcgcaacct ggttgaaaag caagcaaaga ataaggaact
      181 tttccaataa gccaaccacc ttccagccaa taattcaact gtgcccagtt gaattgggta
      241 ttttgtttta taattcctac caatttcccg taattattta cttgattacc aactttatat
      301 ttccctcctt caagaatcgt ataaattatt ggtttatgat tgtacatata aatgcgttgc
      361 aggtgatctt caaacgtttt ttcatcaaaa gaaaaacgcg tagaaaaaat tatcgattgt
      421 tgatgagtac ttgagtaaga aacattatct cttgtaaaag gtataaattc atttgaaaat
      481 tgctggatag tccgaaaata aatacttgat tctacattag tattccaatc atttgaaaaa
      541 gaaaatgtta actcttttct ttcatttagt cgttgagcct ttctaaagga aaaaatagtg
      601 ctggcaatat cttcatcgcc gctatttaaa gggttttcca gataaagaaa attattgtag
      661 ttgtagtcaa ttcttcgatt atcgttcgtg taatttatac ccaatacacg ttttgtctcg
      721 ccaggcaaac gatattgagc aaaagtagaa tattttactt cttgattgcc aaaaccataa
      781 cccaaataac caccaacagt aaaatttttg cataattttt catttgttct taaaggtaaa
      841 tttactctcc atccttccag cccagttgtt ctggctaaaa attgaatttt gccaatatct
      901 attttcccta ctgaaagata agctgaaaaa atggcatccg acgtccagtt ggtcaatttc
      961 attagttttg tattttgaaa ttgattcaat ttgtcattta atacttctgt agaataacca
     1021 ctttgagcaa aattattttt ataagcaaca gtagtatttt gttgataagt agtggtacga
     1081 ttgaaaaaca aaggaaattc acttgtaatt ttttttacta aattgttatc cttaaaagtg
     1141 attaaaattt tttcctttga gcgacaccat ttattttctt ggtttttttc aaatttttga
     1201 aatatcgtta agtttttgat aaaacttttg tctaattttg aagtagaagc ttctatataa
     1261 gtaatagccc atgtggctga atcaacttga aacttgcctt caaaagcaaa atgtctttga
     1321 ttttttgaag caaaatgaat ttcataaagt ttgcctgtat cacttaaaat actatctgtt
     1381 aaataaaaat gataataaaa ctctgccttt tctgataaag gactaatgta tttttgtcca
     1441 aaaaaagtaa ctgttggctt ataaaaattt aaggaaggat ttatttcacc caatacttga
     1501 ctaattatca tttcttcagt ttgaggagaa gaaaaattgg tttttgaaat ttctttcttt
     1561 ccttttgaag tatattggta ttttacttcg ttcatataca taggaactac taaaaaagaa
     1621 tctgtaacac ttgcattttg caaataatga taaatttgtg ggtcaatgtt ttgtttatga
     1681 attttattta gaattaaaag attttgctca atagcaataa ctttatagtc aggtgaattg
     1741 gtttcatcat tatcttcaag atggtttcta atctgactta taataggtat ggctgcctct
     1801 ttttctttac tagtgaagga attattaatg gtaacagctt ctggttccat ttctactaat
     1861 atccctatgc tttcgccagg cttaattttt atttccttgt ttttataacc cattgctgaa
     1921 aattccaata cgttttcttt gcctgaattt ctgattaaaa aatacccttc ttcattgctc
     1981 acagcaattt ttgaagtgtt cttaaaaaaa atcttagcat ttgcaatggg ttgcttattc
     2041 acttgattaa caatttggcc gactaccagt gtttcttgag ctgacaaaga agtaaaaggg
     2101 aatgagaaca tcaacatcag gtaaacaata aaataaggta tgaatatttt attattcctc
     2161 attttttgca ttgttggtca ttttagggtg atgttgattc aaaggatgaa aattcaaaat
     2221 agtttctcgc aaataatgaa tgtcaatatg ggtataaatt tctgtagtgg taatagattc
     2281 atgtcccaac aactgttgaa tagcccttaa gttggctcca ttttccagca aatgtgtagc
     2341 aaacgaatgc cgaaaagtgt ggggacttac cttctttttt attcctgcag ctgctgccaa
     2401 atctttaaca atggtgaaaa tcattgctcg agtcatttta gtgcctcgtc tgttcaaaaa
     2461 aagataatct tcattccctt ttttgatggg cagtttgttt cgatctattt tccacatttc
     2521 aatttgctca atggaagtag gcgacaaagg aaccagtcgt tgtttactgc ctttaccttc
     2581 caccaacata taaccttctt tgagattcat ttttgaaatt tgtaaaccaa ttaattctga
     2641 aacgcgaatt cctgagccat acaaaacttc aataatggct ttgtttcgat gaccttcagg
     2701 tttacttaag tcaatagcag caaccatagc attgatttcg cttacggaaa gcacttcagg
     2761 cagccgcaaa ccaattttag gactttcgag taattcggta gggtcttcgg taataatatt
     2821 tttataaatc aagaaatgat aaaaagaagt aataccggag ataatgcgtg cctgcgaacg
     2881 aggatgaatt cctgagtccc gaatttcaat gagaaaatca cgaagatgct cggtagtagc
     2941 ttgttccaaa ggtatgtgag cctctgacaa atattgtgat agctttgaca agtctctttc
     3001 ataagcatct atcgtattgt ctgacaagcc tttttcaagt tttaaataat aatgatattc
     3061 atctaaaatc agaagcatac ataaaaatgt agatagtaga ataatttgca aagatataaa
     3121 aagaagagtg aatcaataaa gagttgtttt ttcatctttt tcgcacaata tctatccata
     3181 aagaagaatt ataaaccgct acataattaa atattagagt tataaatatg ctatggttaa
     3241 tttttggtaa ggtgaaagga aatttttatt atgtaatgat agcaaatgtt tttaatttat
     3301 ttcaatattc tagaaaatta cttttcataa gataaacaat gacagggtaa tgtaagagta
     3361 tctttaaact tgtaagtgaa cgttatattc aaatttgcca aagtatcttt tcttttagaa
     3421 tattgagtaa taataccatc aagataatca gaaaaagaat aaatccatcc taattctgca
     3481 cctatcgaaa aattagaagt aagatgatat tgataaccta tacctactgg gataataggt
     3541 gtcgtatctg tgagtttaac tttatcgtaa ggtctatccg atggagtttc agaaatagat
     3601 agtccttttc tccataattt tgaatcgcta tttaaaatac ctccacctaa ataaatatac
     3661 aacgtgtgta aattcaattt ataaggatca aaaccaccta aaataacgta ttcagcttgc
     3721 aaagaaaagc caactacttt atttttataa gaataatctc tcatttcatt tcttgaatta
     3781 gcatcatctc ctacaaatga tccatacaat acatttattt tataacctaa atcattttta
     3841 aatgactgtc ggagtcctaa agaaactaaa taacgcgtat tagaaatatc ccaatcatta
     3901 atgccattta aaaattgttc ctgataggac ccaccaatat caccaaaaca atatgccaag
     3961 cctacagagt ttaaaacaac agacatagaa ctaccacctc ctgtatttat taatcctttt
     4021 ttttgactat aaatagggaa caaacacaaa atgacaagct gaattataac taaaaatttt
     4081 ttcacagcat taaatcttta aacttgtaag tgaacgttat attcaaattt gccaaagtat
     4141 cttttctttt agaatattga gtaataatac catcaagata atcagaaaaa gaataaatcc
     4201 atcctaattc tgcacctatc gaaaaattag aagtaagatg atattgataa cctataccta
     4261 ctgggataat aggtgtcgta tctgtgagtt taactttatc gtaaggtcta tccgatggag
     4321 tttcagaaat agatagtcct tttctccata attttgaatc gctatttaaa atacctccac
     4381 ctaaataaat atacaacgtg tgtaaattca atttataagg atcaaaacca cctaaaataa
     4441 cgtattcagc ttgcaaagaa aagccaacta ctttattttt ataagaataa tctctcattt
     4501 catttcttga attagcatca tctcctacaa atgatccata caatacattt attttataac
     4561 ctaaatcatt tttaaatgac tgtcggagtc ctaaagaaac taaataacgc gtattagaaa
     4621 tatcccaatc attaataccg tttaaaaatt gttcctgata ggacccacca acatcaccaa
     4681 aacaatatgc caagcttaca gagtttaaaa caacagacat agaactacca ccagtactta
     4741 ttaatccttt tttttgacta taaataggga ataagcataa aatgacaagt tgaattataa
     4801 ctaaaaattt tttcatagta ttaatttttt gaacttgttt aataattgat acgaaatact
     4861 attaattttt attgaaaaat ttttcagtct ttttttacta atatctaaaa cta
//
LOCUS       DAOTKS010000085         4892 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_15145_length_4892_cov_3.626421, whole genome shotgun sequence.
ACCESSION   DAOTKS010000085 DAOTKS010000000
VERSION     DAOTKS010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15145_length_4892_cov_3.626421"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /gene="glnS"
                     /locus_tag="P5236_02685"
     CDS             complement(<1..1017)
                     /gene="glnS"
                     /locus_tag="P5236_02685"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HRU63301.1"
                     /translation="MIYKEEPSKRNLNFIEAIVEQDLKEGKNGGRIQTRFPPEPNGYL
                     HIGHAKAICLDFGIAEKYGGICNLRFDDTNPLKEDVEYVDSIMEDIQWLGFQWKNVYY
                     ASDYFQKLWDLAVFFIKKELAYVDEQSAEIIAKQKGTPTIPGIESPYRNRPVEENLEL
                     FYKMSSGEIPEGAMVLRAKIDMSSPNMHMRDPIMYRIITSHPHHRTGWKWKVYPMYDF
                     AHGQCDYFEGVTHSLCTLEFEVHRPLYDWFIDQYQDSDYRPRQIEFNRLNLTYTILSK
                     RKLLQLVQEGLVNGWDDPRMPTLCGLRRRGYTPEAIRNFINKIGYTKFDALNDIGLLE
                     YSARE"
     gene            complement(1130..1909)
                     /gene="lpxA"
                     /locus_tag="P5236_02690"
     CDS             complement(1130..1909)
                     /gene="lpxA"
                     /locus_tag="P5236_02690"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HRU63302.1"
                     /translation="MEQPLVYIHPEAKIDPSVTVGPFVFIDKNVEVGEGTTIGPHVTI
                     LEGSRIGKNCKIFPGAVIGAIPQDLKFRGEETLAIIGDNTTIREYVTVNRGTASKGKT
                     VIGNNCLIMAYCHIAHDCVIGNNVIMSNASQLAGEVVIDDYAILSGGVLVHQFTHIGS
                     HVIIQGGSKVNKDIPPYIKAGREPLSFEGINSIGLRRCGFTSEQICSIQEIYRYLYLS
                     GMNVSQAIERIEAELPATKEREDVLVFIRNSERGIVKAYFE"
     gene            complement(1927..3309)
                     /locus_tag="P5236_02695"
     CDS             complement(1927..3309)
                     /locus_tag="P5236_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HRU63303.1"
                     /translation="MTKQKTIKETFTLQGKGLHTGLEISLTFIPAVENHGIKLKRIDL
                     PEQPIIEALAENVTHTIRGTVLQKGEVQISTVEHALAALYALGIDNCLLEVNGPEFPI
                     LDGSAKHYVEKILEVGIEELNAKKDEYIVRKKIEYSLPDSKSKITLLPDDIFSIDVHI
                     GFDSPVLSNQFASLDSLDNFVTEIASARTFVFVHEIEPLLNMNLIKGGDLKNALVIYD
                     KEISQERMDFLTEKLNQKPIDASKLGYINNELNYPNEPARHKLLDVIGDLSLIGKPIK
                     GKVIANYPGHKINTEFAKLIRKEIKKQEVPVPIYDDSIPPVLDINAIRKLLPHRWPFL
                     LVDKIIEIHDKVIVGIKNVSANETFFVGHFPNEPVMPGVLIVEAMAQTGGILVLNSIP
                     HPEKYSTYFLRIDNVKFRQKVVPGDTVIFRLEMIDEIRHSCTNMKGHAFVGGKIVAEA
                     QFMAQITKNN"
     gene            complement(3359..4417)
                     /gene="lpxD"
                     /locus_tag="P5236_02700"
     CDS             complement(3359..4417)
                     /gene="lpxD"
                     /locus_tag="P5236_02700"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRU63304.1"
                     /translation="MEFTAQQIADFLGGEIEGNPSVKVRGFSKIEEGKPGTLTFLSNP
                     KYNHYLYDCKASIVLINKDFKPEKEVTATLIRVDNAYESLAKLFSLINQNQIHKKGIS
                     SLAYVAPTAQIGENVYIAPFVFIGENVKIGNNSEIYAQCTIEENSTVGENVILYPGVK
                     IYHNCVIGNNCILHSGVVIGADGFGFALSKEGIYEKIFQLGNVVLEDDIEVGANTTID
                     RATLGSTIIHKGVKIDNLVQIAHNVEIGENTVIVAQTGIAGSTKIGKNCVLAGQVGIV
                     GHLQIADNTQIGAQSGVTNSVKTPGQIIIGSPAIPIRNFRRSSILFKNLPEIQNLLIE
                     LKNKVEKLEKNIQSGNSQ"
     gene            complement(4531..>4892)
                     /locus_tag="P5236_02705"
     CDS             complement(4531..>4892)
                     /locus_tag="P5236_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HRU63305.1"
                     /translation="ICAIKVWTNNEDKVLSYLCKSFINRKLFKVEMLEHGFHETKRQH
                     LLSQYQEYFKISAEEAEYFMGEETVSTDTYNPGEERINILYKNNTVKDITEASDMLNI
                     KVLTKKVYKHYLCYCRI"
BASE COUNT         1554 a          896 c          732 g         1710 t
ORIGIN      
        1 ttcacgtgct gaatactcca gcaaaccaat atcgtttaat gcatcgaatt tagtataacc
       61 aattttgttt ataaagtttc gaatagcttc gggagtgtat cctcttcttc taagtccaca
      121 aagagttggc atgcgaggat cgtcccaacc gttcaccaga ccttcctgca ccagttggag
      181 taattttctt ttacttaaaa tagtataggt tagatttaaa cgattaaatt caatttgtcg
      241 tggtcgataa tctgaatctt gatactgatc aatgaaccaa tcataaagtg gacgatgaac
      301 ttcaaattca agtgtgcata gagaatgtgt aactccttca aaataatcac attgcccgtg
      361 agcaaaatca tacatcggat acactttcca tttccaacct gtgcgatgat gaggatgcga
      421 ggtaataata cgatacataa ttggatcgcg catgtgcata ttgggcgaag acatatcaat
      481 ttttgctcgc aataccattg ctccttcagg aatttcacct gatgacattt tataaaacaa
      541 ttcaagattt tcttcgacgg gacgattacg gtatgggctt tcgataccag gaatagtagg
      601 cgttcctttc tgttttgcta tgatttcagc ggattgttca tcaacataag ccaattcttt
      661 tttgataaaa aaaacagcta aatcccaaag cttttgaaag taatcagaag cataatatac
      721 atttttccac tgaaaaccaa gccattgaat atcttccata atggaatcaa cgtattcaac
      781 gtcttctttt aatggattgg tatcgtcaaa acgtaaatta caaatgccac catatttttc
      841 ggcaatgcca aaatcaaggc aaatagcttt agcatgtcca atatgtaaat aaccattagg
      901 ttcaggtgga aaacgtgtct gaattctacc tccatttttc ccctccttta aatcttgttc
      961 aacgattgcc tcgataaaat tcaagtttcg tttggaaggt tcttctttgt atatcatata
     1021 tttattactc aaattatttt tatatcaacc tcttttaaag ataattgatg tagatttgaa
     1081 aaaagaataa aaaagatttt ttgatcgttt gatagaaaga gtaaaatatt tattcaaagt
     1141 aagccttgac aatacctctt tcggaattac ggataaatac taaaacatct tcccgttctt
     1201 ttgtggcagg caattcagct tcgatccgtt caattgcttg cgaaacattc atccctgata
     1261 aatataaata gcgataaatt tcctgaatac tacaaatttg ttcacttgta aaaccacatc
     1321 gacgtaaacc aatagaatta ataccttcga atgaaagtgg ttcacgacct gcttttatat
     1381 aaggaggaat atctttattt acttttgaac cgccttgaat gatcacatgc gaaccaatat
     1441 gggtaaattg atgtactaac acgcccccgc ttaaaattgc ataatcatct attactacct
     1501 cacctgccag ttgagaagca ttcgacataa taacattgtt cccaataaca caatcatgag
     1561 caatatgaca gtatgccatt atcaaacaat tgttgccaat tacagttttt ccttttgagg
     1621 ctgtaccgcg atttacggta acatattcgc gaatggtagt gttatcgcca ataatggcaa
     1681 gagtttcttc ccctctaaat tttaaatctt gcggaatagc tccaattact gcgccaggaa
     1741 aaattttgca atttttcccg attctacttc cttcaagtat agttacatga ggaccaatgg
     1801 tagtcccttc tccaacctct acgtttttat caataaaaac aaaaggtcct actgtgactg
     1861 atggatctat tttagcttct ggatgaatat agacaagcgg ttgttccatt ttttctatat
     1921 caacttttaa ttattttttg taatctgagc cataaattga gcttctgcta caattttccc
     1981 gccaacaaaa gcgtgtcctt tcatgttcgt acagctatgt ctgatttcgt ctatcatctc
     2041 aagacgaaag ataacagtat cgccaggaac cactttctga cggaatttta cattatctat
     2101 tcttaaaaaa taggtagaat atttttcagg atgaggaata gaattcagaa ctaaaattcc
     2161 acctgtttga gccattgctt ctacgatcaa aactcccggc atcacgggct cgttaggaaa
     2221 atgacctaca aaaaaagttt cgtttgctga aacatttttt attcctacaa taactttatc
     2281 atgtatctca ataattttgt ccaccaaaag gaaaggccat cggtgaggta aaagctttct
     2341 gatggcattt atatccaata caggaggaat gctatcatca taaataggaa caggaacttc
     2401 ttgctttttt atttctttac gaattaattt ggcaaattct gtattgattt tatgtcctgg
     2461 ataattggca attactttac ctttaatggg tttaccaatc agcgacaaat caccaatgac
     2521 atcaagaagt ttatgtcttg ctggttcatt tggataatta agttcattat taatataacc
     2581 taatttggaa gcatcaatag gtttttgatt taatttctca gtaagaaaat ccattctttc
     2641 ctgcgagatt tccttgtcat aaattaccag tgcatttttt aaatcccctc ctttaataag
     2701 gttcatgttc aataaaggtt caatttcatg aacaaaaacg aaagtgcgag cagaagcaat
     2761 ttctgttaca aaattatcca gtgaatcaag tgaagcaaat tgattggaaa gcactggtga
     2821 atcaaatccg atatgaacat caatgctaaa tatatcgtcg ggtaataaag taatttttga
     2881 tttagaatct ggcaaagaat attctatttt ttttctaaca atatattcat cttttttagc
     2941 attcagttct tctattccaa cctccaaaat tttttcaaca taatgtttgg cactaccatc
     3001 taaaatagga aattctggtc cattaacttc cagcaaacaa ttgtcgattc ctaaagcata
     3061 caaagctgcc agtgcatgtt ctaccgtact tatttgaact tctccttttt gcaaaacggt
     3121 accacgaatt gtatgggtta cattttctgc taaagcttca atgatgggtt gttcaggaag
     3181 gtctattcgt ttcagcttaa ttccatgatt ttcaactgct ggtataaatg ttaaagagat
     3241 ttctaaacct gtatgtaatc cttttccttg aagagtgaat gtttccttta ttgttttttg
     3301 ttttgtcatc attttacttg ttagaataaa aatcctttat gtgaggcagt actatgaatt
     3361 attgagagtt acctgattga atattttttt caagtttttc cactttattt ttcaattcta
     3421 taagaagatt ttgtatttcc ggtaaatttt taaaaagaat agaagacctt ctaaaattac
     3481 gaatgggtat agcaggagaa ccaataataa tctgacctgg tgttttcact gaatttgtta
     3541 cacctgattg tgctcctatt tgtgtattgt ctgcaatttg taaatgtcct acaattccta
     3601 cttgcccggc taaaacacaa tttttaccta tttttgttga acctgcaatc cctgtttgtg
     3661 ccacgataac agtattttct ccaatttcga cattatgagc aatctgcacc aaattgtcta
     3721 tttttacccc tttgtgaata atagtactac ccaatgtagc tctatcgatg gtggtatttg
     3781 ctcccacttc aatatcatct tccaacacaa cgttgcctaa ttgaaaaatc ttttcgtaga
     3841 ttccttcttt ggatagtgca aaaccaaaac catcggctcc aatcacaaca ccactatgca
     3901 aaatacaatt gttaccaata acacaattat gataaatttt cactcctgga taaagaatta
     3961 cattttcgcc aactgtagaa ttttcttcga tagtacattg agcatatatt tcagaattat
     4021 ttccaatttt aacattttct ccaataaaca caaaaggagc aatataaaca ttttcaccaa
     4081 tttgtgccgt aggagcaacg tatgccagtg acgaaatgcc ttttttgtgg atttgatttt
     4141 gattaatgag agaaaaaagt ttagctaatg attcataagc attatccaca cgaattaaag
     4201 tagcagtaac ttctttttca ggcttaaaat ctttgttgat taaaactata ctggctttac
     4261 aatcataaag ataatgattg tatttgggat tggacagaaa agtaagcgtg cctggctttc
     4321 cttcttcaat tttagaaaaa ccccttactt ttacagaagg attaccttca atttctcctc
     4381 ctaaaaaatc tgctatttgt tgagcggtaa actccatagt taaaaatttt ggctacaaaa
     4441 gtactaaact atttttaatt tttcagtttt agaaataaac aattttaaat aataagatat
     4501 aaactatttg ttattaaata tttatataat ttatattctg caataacaca aataatgctt
     4561 ataaactttt ttggtaagca cttttatgtt caacatatcg gatgcttcgg ttatgtcttt
     4621 gacagtatta tttttgtata agatattaat tctttcttcg ccgggattgt aagtatctgt
     4681 actcaccgtt tcttctccca taaaatattc cgcttcttcg gcagaaattt taaaatattc
     4741 ttgatattga cttaacaaat gttgtcgttt agtttcatgg aaaccatgtt ccaacatttc
     4801 aactttaaat aattttctat taataaacga tttacataaa tatgataaaa ctttatcttc
     4861 gttatttgtc caaactttaa tggcacaaat ca
//
LOCUS       DAOTKS010000086         4846 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_15301_length_4846_cov_5.584847, whole genome shotgun sequence.
ACCESSION   DAOTKS010000086 DAOTKS010000000
VERSION     DAOTKS010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15301_length_4846_cov_5.584847"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="P5236_02710"
     CDS             <1..544
                     /locus_tag="P5236_02710"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRU63306.1"
                     /translation="NNFKECLEGLRKGNYDIYADFIPITSEWQNEYLFTIPLKTSRQV
                     LIQCKDSSEKIIKNQVDLAGDTIYIPKNSPHKLRLKNLSDEIADTIYLVEVNDVNSED
                     LVKWVAMGKIKQTICDERDALKWKLQYPNLDISLPVGFDQQYGWMLYKESKQLQKKLN
                     DFLNDFIGSEAYWKIYRKYY"
     gene            635..1552
                     /gene="metA"
                     /locus_tag="P5236_02715"
     CDS             635..1552
                     /gene="metA"
                     /locus_tag="P5236_02715"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HRU63307.1"
                     /translation="MPLNIPASMPAVEILRKENIFVMDSDRASHQDIRPLKIVILNLM
                     PLKITTETDLIRLLSNSPLQIEVDFLQLESHTPKNTPIEHMMAFYKKFDVIKESNYDG
                     MIITGAPIEHLSYEEVTYWDEITKIFDWVRTHVTSTMFICWAAQAGLYYYYGIPKYPL
                     KEKMFGVFEHTLYNPQNPIFRGFDDVFYVPHSRYTEVRAEDIKKVPELTLLSESAESG
                     VYMVMGRNGREFFITGHSEYSARTLDDEYKRDLAKGLPIKMPKNYYLNDNPENEPVVR
                     WRSHANLLFTNWLNYFVYQETPYDLSKIH"
     gene            complement(1676..2635)
                     /locus_tag="P5236_02720"
     CDS             complement(1676..2635)
                     /locus_tag="P5236_02720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRU63308.1"
                     /translation="MYQSLSIIVPCYNEEEVITESYKRLRQVSQKLDFDTDIIFINDG
                     SIDRTEQILSEIAKTDKNVKVISFSRNFGHQNAVTAGLNHCTSDLAVIIDADLQDPPE
                     LIPQMIETLKKNQAQVVYCVRKIRKGESLFKRFSAKIFYRFLNFLSEIKLPVDTGDFR
                     LVDKNIIKEFNRMREKGKYIRGLIAWMGFKQVPFYYEREARFAGETKYSLSKMIKLAT
                     TSMLYFSKKPLKIAIYLGFFTTLLAILLVLWSIFGKIFGFTNADPGWTSLFIAIVFFG
                     GIQLLTIGILGTYIGTLFDEVKDRPEYVVKNLLNFENNIEKEK"
     gene            complement(2642..3028)
                     /locus_tag="P5236_02725"
     CDS             complement(2642..3028)
                     /locus_tag="P5236_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HRU63309.1"
                     /translation="MNYSKIVIQFFKFGIVGILNTLITAFIIFLLLKVFHCSDYVSNI
                     IGYLAGLINSFIWNRKWTFESHGKISVTVFKFIITFVISYLLQLGGLHLLLQYTTIDN
                     YVCQLIAMVIYTIINFLLNKYYTFKI"
     gene            complement(3179..4621)
                     /locus_tag="P5236_02730"
     CDS             complement(3179..4621)
                     /locus_tag="P5236_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63310.1"
                     /translation="MSLKIRKQFLFIFFIISIYTLLTIVCWNHCYFWDNIQQISKEAH
                     WFYQNNFSSLIIPIHSSSDEIVPTGYHPPLMGIMTAALWKIFGYHLWVSHFFVFCWFF
                     ILLYQTIKLLQLIFPQKYASGALIIVLLEATVLTQFAIASPDFILLTAFVICLRAILE
                     QKKILLSVGLIFLCGINARGLLTGIALFFTNYYYLYLQSEKKHTFECLKKSFIPFLPT
                     LLLLIAYYCLYFKTNGWFFADSSEAGHYALPANFSMIIKHLFEFIIRLIENGRIVVWG
                     VAFYLFILIIRGKIQTSIKDNVLIFCFLFLMAEYLVLVFTTRMPFSGRYFMPLFFVLT
                     LITLSGITHFFDRKKIKITFVVILLFELTGHFWIYPEKIAQSWDSTLAHLPYYELRKN
                     CFDYIDQQGIDYQKISAGFCLYGDRGYIELQHAGKKVNDEFNNEYFIYSNISNIADEW
                     LFQLKDTTQWKPIQTFQKWPVKIILYQKIQ"
BASE COUNT         1763 a          749 c          758 g         1576 t
ORIGIN      
        1 taataatttt aaagaatgtt tggaaggttt aaggaaaggc aattatgata tttatgctga
       61 ttttattcct attacttccg aatggcaaaa tgaatatttg tttactattc ctcttaaaac
      121 ttccaggcaa gtattgatac aatgcaagga ttcatctgaa aaaattatta aaaatcaagt
      181 ggatttagct ggcgacacca tttatatacc aaaaaattca cctcataaac tacgattaaa
      241 gaatctttct gatgaaattg ctgatacaat ttatctggtt gaagtaaatg atgtcaatag
      301 tgaagattta gtaaaatggg tagctatggg taaaatcaaa caaactattt gtgatgaaag
      361 agatgcatta aaatggaaat tacaatatcc aaacctcgat atttctttac cagtcggttt
      421 tgaccagcaa tatggttgga tgttgtataa agaatcaaaa caattgcaga aaaaattaaa
      481 tgattttctg aatgatttta ttggaagtga agcttactgg aaaatttatc gtaaatatta
      541 ttaaatttga actttgtttt tgtaaggttg ttttaataat caaacaatta cgaatcagct
      601 tatttgaata aaaaaatttg aaaaaaataa aaatatgcca ttaaatattc ctgcctcgat
      661 gcccgctgtg gaaattctca gaaaagaaaa catttttgta atggattctg atagagcttc
      721 tcatcaagat attcgtcctc ttaagattgt gatattaaac ttgatgcctt taaaaattac
      781 gactgaaact gatttgatac ggttgctttc gaattcaccg cttcaaattg aagtcgattt
      841 tttgcaattg gaaagtcata cccccaaaaa tacgccaatt gagcacatga tggcttttta
      901 caaaaaattt gatgtcatca aggagagcaa ttatgatgga atgattatta caggtgcgcc
      961 catcgaacat ttatcttatg aggaagtgac ttactgggat gaaatcacga aaattttcga
     1021 ttgggtgcgt acgcatgtca cttctactat gtttatatgt tgggcagccc aggctggttt
     1081 gtattattat tacggcattc ctaaatatcc attgaaagaa aaaatgtttg gcgttttcga
     1141 acatacgcta tataatccac aaaacccaat ttttcgaggt tttgacgatg tattttatgt
     1201 tcctcatagt cgttacactg aagttcgtgc cgaagatatt aagaaagttc ccgagcttac
     1261 tttactttca gaatccgcag aatcaggtgt ttacatggtg atggggagaa atggacgaga
     1321 gttctttatt actggacatt ctgaatattc agctcgaaca ctggatgacg aatataaaag
     1381 agatttagca aaagggttgc caataaagat gcctaaaaat tactatttga atgataatcc
     1441 agaaaatgaa cctgttgttc gctggagaag tcatgctaat ttgcttttta caaactggct
     1501 taattacttt gtttatcagg aaacaccata cgatcttagt aaaattcatt gaattgaaat
     1561 tcatcagggc aaataaaagg aaaaatattt ttcatcttat gttcctttaa aagctgcatt
     1621 aattggtgga atttgcttga attatgagaa ttgtaaatag ctaagcttat tgtagttatt
     1681 tttccttttc tatattattt tcaaaattca gtagattttt cactacatat tcaggtctat
     1741 ctttcacttc atcaaaaagg gttcctatat aagttcctaa aattccaatc gtaagtaatt
     1801 gaattccacc aaaaaacacg atggctataa ataaggatgt ccatccagga tcagcatttg
     1861 taaaaccaaa tattttccca aatatcgacc acaaaaccaa taagatagcc agtaatgttg
     1921 taaaaaatcc gagataaata gctattttta atggcttttt agagaaatac agcatggaag
     1981 tagttgccaa tttaatcatt ttagaaagcg aatattttgt ttccccagca aaacgggctt
     2041 ctctctcgta ataaaaaggt acttgtttaa atcccatcca ggcaattaaa ccacgaatat
     2101 attttccttt ttccctcatt cggttgaatt ctttaataat atttttatcc accaatctga
     2161 aatcacctgt atcaacggga agttttattt ctgacaaaaa attcagaaat cgataaaata
     2221 tttttgctga aaacctttta aataaacttt ctccttttcg aattttacgt acacaataca
     2281 ctacttgagc ctgatttttt tttagtgttt ctatcatttg cggtattaac tcgggagggt
     2341 cctgtaaatc ggcatcaata attacggcca aatcagaagt acaatgattt aaacccgccg
     2401 tcactgcatt ttgatgtccg aaatttcttg aaaagctaat tacttttaca tttttatccg
     2461 ttttcgctat ttctgataaa atctgttcag tacggtcgat acttccatca ttgataaaaa
     2521 tgatatctgt atcaaaatct aatttttgtg aaacttgcct caatctttta taagattcag
     2581 taattacttc ttcttcatta tagcaaggaa cgattatgct taatgactga tacatttaaa
     2641 tttagatttt aaaagtataa tacttgttta ataaaaaatt aataatggta taaatcacca
     2701 ttgctatcaa ctgacaaacg taattgtcta ttgttgtata ttggagcaat agatgtaagc
     2761 cacccaattg taataaatag gaaataacaa aagtaatgat aaatttaaaa actgttactg
     2821 aaatttttcc atgactttca aaggtccatt ttctgttcca gataaaacta tttatcaaac
     2881 ctgcgagata gccaataata tttgaaacat aatcagaaca atgaaaaacc ttgagcaaca
     2941 aaaagataat gaatgcagta atgagtgtgt ttaaaatacc cactatacca aatttgaaaa
     3001 attgtatcac aattttagaa tagttcatag caggaaaaat ttaatcgtaa gaattccccg
     3061 atgcttgcgt cggggtagct tttgttttta tttttttaga aattccggtg gcgaagccat
     3121 aaaattggcg taatacctcg ctgcttgcgg cgaggatagt caattttaag gaatttcttt
     3181 attgaatctt ttgatataaa attattttaa ctggccattt ctgaaaagtt tgtataggtt
     3241 tccactgagt ggtatctttc aactgaaaga gccattcatc agcgatatta gaaatattgg
     3301 aataaataaa atactcatta ttgaattcat catttacttt tttgcctgca tgttggagtt
     3361 ctatgtaacc tctgtcgcca tacaaacaaa aacctgcaga aattttctga taatctattc
     3421 cttgctgatc aatataatcg aaacaatttt ttctcaattc ataataaggc aaatgagcca
     3481 gcgtactatc ccacgattga gctatttttt caggatagat ccagaaatgt ccagttaatt
     3541 cgaacagcag aattacaaca aaagtaattt taattttttt acgatcgaaa aaatgagtaa
     3601 tccctgatag agtgattaaa gtcaacacaa aaaacaaggg catgaaatat cttcctgaaa
     3661 aaggcattcg cgttgtaaaa accaaaacaa ggtattctgc cattaaaaat aaaaaacaaa
     3721 aaatcaatac attatctttg atgctggttt gtattttccc tcgaataatc agtataaaca
     3781 aataaaaagc aactccccaa acaacaatac gtccgttttc tataagtcgg ataataaatt
     3841 caaaaagatg ttttattatc atggaaaaat tagcaggtaa agcataatgt cccgcttctg
     3901 aagaatcggc aaaaaaccat ccattggttt taaaatacaa gcaataatat gctattaata
     3961 acagaagagt aggaagaaaa gggataaaag attttttcaa acactcaaag gtatgttttt
     4021 tctctgattg taaatataaa taataataat ttgtgaaaaa caatgcaatt cctgttaaca
     4081 agcctctggc gttaatgcca cataaaaaaa tcaaacctac agaaagtaaa attttttttt
     4141 gttctaaaat tgctcgtaaa caaatcacaa atgcagttaa taaaataaag tcgggcgaag
     4201 caatagcaaa ttgagtcaac actgttgctt ccaaaagtac gatgataagt gccccacttg
     4261 catatttttg agggaaaatt agttgcagca attttatggt ttgataaagc agaataaaaa
     4321 accagcaaaa aacaaaaaag tgagaaaccc acaaatgata accaaaaatt ttccataagg
     4381 cagccgtcat tatccccatc aaaggtggat gatatcccgt gggaacaatt tcatcacttg
     4441 aactgtgtat gggaattatt agggaagaaa aattattttg ataaaaccaa tgtgcttctt
     4501 ttgaaatttg ttgaatattg tcccagaagt agcaatgatt ccagcataca attgtcagca
     4561 aagtgtaaat actgataatg aaaaaaataa aaagaaactg tttccttatt tttaaagaca
     4621 taatgaaaaa atttttgata atttttgata agaaaggtaa aaaatttccc cttttctcat
     4681 aaaattattt catttgtttt tgataatgaa ctgtttgttc gaattcctaa ataaaaatca
     4741 atggaaataa agactaaaat taataaataa ataaagaaag ctggccacat aggtagaatt
     4801 tcattgaagc gagagcagcg aatataagaa taaagcgaaa tcaccc
//
LOCUS       DAOTKS010000087         4818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_15405_length_4818_cov_4.165442, whole genome shotgun sequence.
ACCESSION   DAOTKS010000087 DAOTKS010000000
VERSION     DAOTKS010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15405_length_4818_cov_4.165442"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /gene="rsmH"
                     /locus_tag="P5236_02735"
     CDS             complement(<1..464)
                     /gene="rsmH"
                     /locus_tag="P5236_02735"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016271911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRU63311.1"
                     /translation="MNDGAYHIPVLLHEAVNGLNIQPDGIYVDATFGGGGHAREILNK
                     LNEKGRLIAFDQDEEATKNAFNDSRFTFVHSNFCYVKNFLRYLEIDKVNGILADLGVS
                     SHHFDASERGFSFRFEGKLDMRMNKRSPLTAADVLNTYSEEKLADIFFNYGE"
     gene            complement(475..930)
                     /locus_tag="P5236_02740"
     CDS             complement(475..930)
                     /locus_tag="P5236_02740"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRU63312.1"
                     /translation="MSTFIGKYEAKVDAKGRIFVPAAYRKLLGDIEKDRIVMRKEEEN
                     NCLIFYPEAVWNQKIEYLKSKLDEWNSIDELLLMQFVADAEWLVIDTQGRILVSKKNL
                     QTLGIENGEVVFMGMIDRFALWSKAHYEKARLSSDEFTSLLKEKLMKSE"
     gene            complement(2084..2767)
                     /locus_tag="P5236_02745"
     CDS             complement(2084..2767)
                     /locus_tag="P5236_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HRU63313.1"
                     /translation="MLWAYIIFIVVAIVSFIIQNSLQNKFKKYSNLSIPNGMTGKDIA
                     EKMLHDHGIYDVKVTSTSGHLTDFYNPINKTVNLSEQVYDSSNVAAAAIAAHECGHAV
                     QHAEAYAPLTLRSKLVPVVSFASSWMQWVLLAGIIFLNTFPQLMLAGIILFATTTLFS
                     FITLPVEINASQRALLWLNNAGITNHETHEPAKDALKTAAYTYVVAALGSLATLVYYI
                     LIFIGGSRR"
     gene            complement(2914..4185)
                     /locus_tag="P5236_02750"
     CDS             complement(2914..4185)
                     /locus_tag="P5236_02750"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRU63314.1"
                     /translation="MKIDVLLGLQWGDEGKGKIIDVLTPHYDIITRFQGGPNAGHTLE
                     FEGKKFILKSVPSGIFQGNKINIIGNGMVLDPALFKAEGEALETAGSPLTKLLKISKK
                     THLILPTHRLLDQAYENAKGDGKIGTTGKGIGPAYTDKVSRNGLRVGDIYHDFQNKYK
                     TAIGRHLDLLKFYQVDVDLSALEKEWFEGIEYLKRFELIDSEYYINKALSVGKKVLAE
                     GAQGTMLDIDFGTYPFVTSSNTICAGTCIGLGVAPSCIGEVYGIFKAYCTRVGSGPFP
                     TELKDETGEIMRKRGDEYGSVTRRPRRCGWIDLVALKYAIMLNGVSQLIMMKSDVLDN
                     FETIKACVAYKIDGKETTQFPFEVADNIEPVYVELAGWKTDLTKIKEEEKLPDNFKSY
                     LNFLEKELKIPIKIVSLGADREQTIVRNVCK"
     gene            complement(4263..4739)
                     /locus_tag="P5236_02755"
     CDS             complement(4263..4739)
                     /locus_tag="P5236_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004290125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRU63315.1"
                     /translation="MKEENTYETVKEVFTEYLEKHQHRKTPERYAILEQIYAHEGHFN
                     AELLYNAMQKDFRVSLATIYNTLDLLLECHLIVKHQFGDQLAQYEKTFGTNIHHHLIC
                     TQCGKIKEFSDKNIRTAIQSKHFSNFQMTHYSLYVYGICSKCQNKKKNRKNKSIKT"
BASE COUNT         1597 a          859 c          704 g         1658 t
ORIGIN      
        1 aattctccat aattaaaaaa aatatcagcc aatttttctt cagaataggt gttcaacacg
       61 tctgcagcag ttaacggaga tcgtttgttc atacgcatat ccagtttccc ttcaaaacga
      121 aaagaaaagc cccgttctga agcatcaaaa tgatgcgatg atacacccaa atctgctaaa
      181 atgccattta ctttatctat ttcaagataa cgtaaaaaat tttttacata gcaaaaatta
      241 ctatgaacaa acgtaaaacg cgaatcatta aaagcatttt tggtagcctc ttcatcctga
      301 tcaaaagcaa ttaatcgtcc tttttcattc agtttgttta atatttcacg agcatgaccg
      361 ccaccaccga aagtagcatc tacataaatt ccatcaggct gaatattcaa cccattgact
      421 gcctcgtgca gcaaaacagg aatatgatac gctccatcgt tcatcaggat ttttttattc
      481 tgatttcatt aatttttctt tcaacaaact ggtaaattcg tcagacgaaa gtcgagcttt
      541 ttcataatga gctttactcc aaagggcaaa acggtcaatc attcccataa aaactacttc
      601 gccattttct attcctaacg tttgaagatt ttttttcgag actaaaatcc gtccctgcgt
      661 gtctataacg agccattcag catctgctac aaactgcatt aataaaagct catcgataga
      721 attccattcg tccagctttg atttcaagta ttcaattttt tgattccaaa ccgcttcggg
      781 ataaaatatc aaacaattgt tttcttcttc tttacgcatt acaatgcggt ctttttcaat
      841 atctcctaaa agttttcgat aagccgcggg gacaaaaatt cttcctttgg catctacttt
      901 agcttcatat tttccgataa atgttgacat taattgagat ttttacttta cactgtaaaa
      961 ataaaaaaat aaatttaaat tccccacatt tccccacaaa aaaattatca acaattaatt
     1021 aacagcaaaa aatgctttta aaaactttat aaacagctta ttgattataa ttcgatcaga
     1081 aaatatttga tttaaaatac ttattatcaa aatgttatga attttatttc ataaaaatgg
     1141 aattaagtgg ggaatatttt tttacttatt aacaacttat taacagataa cttcatccca
     1201 gaattgtcat taggaatata tcatttattt aactgtagtt tttaaagtat tgaaaatcgt
     1261 tttaaatttt ttatacacaa caaacgatga tgtttttcta ttagagttga atttagcgaa
     1321 ttaatgatgt tttttggata gatctcatct gataagccaa aaattgacta atcttgaatt
     1381 ttgagttcgc aagatatgaa gtacaagttt gagttaagag tttggagttc tgaatttgga
     1441 acaaaaaaga aaaaaaatct ctgtgttttt ccgtataata aatcattcat tttcaaaaag
     1501 tttcgcagaa aaggcacgaa gatatagaga aaaatcttaa atgccaagca aagtagaaga
     1561 acaattttat ttgtgttaag ataattaagc gaagcgaact cgctgaaaca actatttcgc
     1621 ttatacagat atttatctgt ttattattct aagagtaaga aaaatattat ttgctttttc
     1681 caagcaagat acaagtaaat acttacgtca ggaatttatc aaaaaagtta gttggattgg
     1741 gagcaaaact cctaatacaa aatgttattc aataaatttt aggcaatttt tgtcttgtag
     1801 ttttttataa aattaagcaa aagctccttc cattcatttt aaaaaaaata agtctaattt
     1861 tgataatgtt ttttcggcac aacatattat aattcaaata attacaattt attttctaat
     1921 aaccgccatt agaaattaaa ttctaatgat aaatttgggt taaaaaaaat tgcattaact
     1981 acttgaattt acgttatgaa agcaagttgg tattaaaaaa aggaaaacct tccataaaaa
     2041 acggcttagt tttccttctt gtaaaagtta cagttttatt tttttatctg cggcttccgc
     2101 ctataaaaat caaaatatag taaaccagcg ttgccagcga tcccaaagct gctactacat
     2161 aagtataagc tgctgttttc aaagcatctt tggcaggttc atgcgtttcg tggttagtaa
     2221 ttccggcatt gttgagccac aacaaagccc gctggctggc gttaatttct accggtaaag
     2281 taataaaact aaataaagtt gttgtagcaa ataaaataat tcctgctaac attaattgag
     2341 gaaatgtgtt gagaaatata atccctgcca gcaataccca ttgcatccac gaagaagcaa
     2401 aactaactac tggtaccagt ttggatcgca acgtgagggg agcataagct tcggcatgtt
     2461 gaactgcatg cccacattca tgagcggcaa ttgctgcagc agctacattg ctcgaatcat
     2521 aaacttgttc acttaaattg actgttttgt ttataggatt atagaaatct gttaaatgtc
     2581 ccgacgtaga agttactttt acatcataaa taccatgatc atgtagcatt ttttctgcta
     2641 tatcttttcc tgtcatacca ttaggaatag ataaattgga atatttctta aacttgttct
     2701 gtaaactatt ctgaataata aagctaacaa ttgcaacgac aataaaaata atataagccc
     2761 acaacataat ttatttgttt aaaagttcga cagcaaataa gcaaaatctt tgccaaaata
     2821 aaaatatgac acagaaattt actcgcattt taagtaaaat aattttcctt ccttccaaat
     2881 cacaatcaat gaatttttca ttgatataat aatttattta caaacatttc taacgatggt
     2941 ttgttcacga tcggcaccaa gtgacactat ttttatagga atttttaact ctttttctaa
     3001 aaaatttaga taggatttaa aattgtctgg caatttttct tcttccttga tcttagttaa
     3061 atcagttttc caaccagcta attccacata aacaggttcg atattgtcgg ctacttcaaa
     3121 aggaaattga gtggtttctt ttccatcgat tttgtatgca acgcatgctt tgatggtttc
     3181 aaaattgtct aacacatcac ttttcatcat gataagttga gaaacaccat tgagcataat
     3241 agcatatttc aaggctacta aatcaatcca gccgcatcgc ctgggtcgtc gagttactga
     3301 accatattca tctccccgtt ttcgcataat ttctcctgtt tcatctttca gctcagtagg
     3361 gaaaggaccg ctgcccacgc gtgtacaata agctttgaaa ataccataga cttctcctat
     3421 gcagcttgga gcaactccaa gcccaataca tgtgccagca caaattgtat tggaagaagt
     3481 tacaaatgga taagtgccaa aatcaatatc cagcatcgta ccttgagcac cctcagccaa
     3541 tacttttttc cccaccgaaa gagctttgtt gatataatat tcactatcaa ttagttcgaa
     3601 acgtttcaga tattctattc cttcaaacca ttctttttca agagcggata agtcaacatc
     3661 tacctgataa aatttaagca agtcaaggtg acgaccaatg gctgttttgt atttgttttg
     3721 gaaatcatgg taaatgtcac ccacacgcaa accgttacga cttactttat cagtataggc
     3781 gggaccaatt cctttacctg tggtaccaat ttttccatca ccttttgcat tttcataagc
     3841 ctgatcgagc aaacgatggg tgggaagaat caaatgtgtt tttttagaaa ttttcaataa
     3901 tttggtcaac ggacttcctg ctgtttccag tgcctcgcct tctgctttga ataaagctgg
     3961 atcgagtacc attccattgc caataatatt aattttattt ccctgaaaaa tgcctgatgg
     4021 aacagatttc agaataaatt tttttccttc aaattctaaa gtatgacctg cattgggacc
     4081 gccttgaaag cgtgtaataa tatcataatg aggagttaaa acatctataa tttttccctt
     4141 gccttcgtcg ccccattgaa gacccaataa tacatctatt ttcatttttg aattctttaa
     4201 ttttttctct ggaattataa aatcactttc gttataaaaa tattttcttt tttgatgaaa
     4261 atttaagttt tgattgattt gttttttctg tttttctttt tattctggca cttgctgcaa
     4321 attccataaa catacaatga ataatgtgtc atctgaaaat tgctaaaatg ctttgactgg
     4381 atggctgttc ggatattttt atcagaaaat tccttgattt ttccgcattg tgtacaaata
     4441 agatgatgat gaatattggt gccaaaagtt ttttcgtatt gtgctaactg atcaccaaac
     4501 tgatgtttga caatcaaatg acattccagc agtaaatcca acgtattata aatggtggca
     4561 aggctaaccc gaaaatcctt ttgcatcgca ttatacaata attcagcatt gaaatgacct
     4621 tcgtgagcat aaatttgctc taaaatagca tatctttcag gcgtttttct gtgttgatgc
     4681 ttttcaagat attcggtaaa aacttcctta acagtttcgt aggtattttc ttctttcatt
     4741 gaaatgaatt cctttcaata aacaaaggta gtattttttt ctgtgttttt tataatagtt
     4801 ccagaaaatt taattctt
//
LOCUS       DAOTKS010000088         4752 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_15651_length_4752_cov_4.150309, whole genome shotgun sequence.
ACCESSION   DAOTKS010000088 DAOTKS010000000
VERSION     DAOTKS010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15651_length_4752_cov_4.150309"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1358
                     /locus_tag="P5236_02760"
     CDS             <1..1358
                     /locus_tag="P5236_02760"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HRU63316.1"
                     /translation="FGMDTKDLPGDGVITGTGTINGAPVCIYAQDFTVMGGSLGIQHA
                     RKITKIMDHALKMKMPCIGINDSGGARIQEGIGALAGYGEIFYRNTIASGVIPQISVI
                     LGPCAGGAVYSPALTDFVFVVENISKMFITGPGVIETVLGEKISQEDLGGARVHAEIT
                     GNAHFYALSEKECFAQIKELISLIPHNNEEKAPGVEPKKPTFKKSIENIIPANPKLPY
                     DVRHIIYALVDNSKFFEVQELWAQNIVVGFARMNGETVGFVANQPLYLAGVLDCDASD
                     KAARFIRFCDAFNIPLITLVDIPGYLPGVDQEHAGVIRHGAKVLYAYSEATVPKMTVI
                     LRKAYGGGYIAMNSRHLGADFTFAWPDAEIAVMGPEGAANIIFKKEIMEAPDPDAMRQ
                     EKVKEYIEKFANPFVAAGKGYIDAVIEPLETRDLLLHALSVSKKKFDYRPAKKHGNIP
                     L"
     gene            1465..1833
                     /locus_tag="P5236_02765"
     CDS             1465..1833
                     /locus_tag="P5236_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005635376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRU63317.1"
                     /translation="MGKNDFQKKNNAKKDETPEFVDFVVTARKYKTLLTKKYLNRKKW
                     ENPSPYEIYSYIPGTIVEVFVKEGEVVEEGEPLLTLEAMKTLNRIEMPFTAKIKKINV
                     KPGDQIPKDYLIIELEPVEE"
     gene            2039..2599
                     /locus_tag="P5236_02770"
     CDS             2039..2599
                     /locus_tag="P5236_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HRU63318.1"
                     /translation="MDQTEQQFDKIVSICKDIFIKKLKDYGASWRLLRPSSVTDQLFI
                     KANRIRSIQMKGISKINEDIKSEFIGIINYGIIGLIQLELGFAEKVDISTEQAVELYD
                     KYIQKSKDLMSNKNHDYDEAWRSMRVESFVDIILMKICRIKEIENHAGKTLISEGIDA
                     NYYDIINYSIFALIKLEEMEKTHNNH"
     gene            2679..3824
                     /locus_tag="P5236_02775"
     CDS             2679..3824
                     /locus_tag="P5236_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRU63319.1"
                     /translation="MTYNSTKKKKSKSHKETKTANFFLELARIILGLVFIFSGFVKAI
                     DPLGSAYKIEDYLIAFGHPFSYFIFLSLPFAILLSTFELTLGFNLILKIYPRITIILT
                     AIFLTALTALTLYLALKNPITDCGCFGDAIILTNWQTFYKNLVLISLAVLLLIYRKRF
                     TPLLLPHIEPFALVIFALLGIVISIYCYHHLPLIDFLPYKVGVNIPEAMHIPEGAKTD
                     QYETTFIYEKNGQRKEFTLENYPKDDSTWVFIDQKTTLKSIGYRPPIHDFVIFDTQDE
                     DITDIILQENNSAYLLIMYDVNKASKVGAKKAEKVYQKAQSTNSFFCAVTASSDEEIQ
                     KFVQATGVTYPFCKADPIMLKTIVRANPGLVLIKNGTIMGKWNWRDF"
     gene            3923..4678
                     /gene="tpiA"
                     /locus_tag="P5236_02780"
     CDS             3923..4678
                     /gene="tpiA"
                     /locus_tag="P5236_02780"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRU63320.1"
                     /translation="MRKNIVAGNWKMNKTLPEGLKLATELNTLLANVKLNCQIIIAPP
                     FIHLASIVAAIDTKKIGVAAQNCADKEWGAYTGEVSAGMVKSTGAEFVILGHSERRTY
                     YGETPEILKTKVELALKYELTPIFCIGEILSEREAGKHFEVVRSQLEKSLFHLPAEEF
                     SKLILAYEPVWAIGTGKNATPEQAQEMHAFIRKTIEERYSSLVADNLSILYGGSCNPG
                     NAKELFANPDVDGGLIGGASLKSQDFKAIIDAF"
BASE COUNT         1648 a          691 c          848 g         1565 t
ORIGIN      
        1 attttggaat ggataccaaa gatttaccgg gcgatggagt aattacagga acaggcacaa
       61 ttaatggagc tcctgtatgt atctatgctc aagattttac agtaatggga ggatcattag
      121 ggatacagca tgctcgtaag ataactaaaa ttatggatca tgccttaaaa atgaagatgc
      181 cttgcatcgg tattaacgac tctggaggtg ctcgtattca ggaaggtatt ggagctttag
      241 caggttatgg tgaaattttt tatcgtaaca ctatagcatc gggcgtaatt ccacaaattt
      301 ctgttatttt aggaccatgt gcaggcggag cagtatattc acccgcgttg actgattttg
      361 tttttgtggt tgaaaatatt tctaaaatgt ttattaccgg tccaggagta attgaaaccg
      421 tcttgggtga aaagatttca caggaagatc tgggaggagc acgggtacat gcagaaatta
      481 ccgggaatgc tcatttttat gcgttgagtg agaaagaatg ttttgctcaa ataaaagagt
      541 tgatatcgtt aatccctcat aataatgaag aaaaagctcc tggagtagaa cctaaaaaac
      601 caactttcaa aaaaagtatt gaaaatataa ttcctgccaa ccctaaatta ccttatgacg
      661 taagacacat tatttatgca ctggtggata actctaaatt ttttgaagtg caggaactat
      721 gggctcaaaa tattgttgtt ggttttgcgc gaatgaatgg agaaacggtt ggttttgtag
      781 ccaatcaacc tttgtatttg gcaggggtat tggattgcga tgcttcagat aaagctgctc
      841 gatttattcg tttttgtgat gcctttaata tcccattaat tactctggtg gatattcctg
      901 gctatttgcc tggtgtagat caggaacatg ccggtgtcat tcgtcacgga gcaaaagttt
      961 tatatgctta ttccgaagca acagtaccaa aaatgacagt tattttaaga aaagcttatg
     1021 gcggtggtta tattgcaatg aattccaggc atttaggagc tgactttact tttgcctggc
     1081 cagatgccga aattgctgtg atgggaccag aaggagcagc caatatcatt ttcaaaaaag
     1141 aaattatgga agctcctgat cctgatgcta tgcgacaaga aaaagtgaaa gaatacattg
     1201 aaaaatttgc aaatcctttc gtggcagcag gaaaaggtta tattgatgcc gtaattgaac
     1261 cgcttgaaac gcgcgattta ttgcttcacg ctctgagtgt atcaaagaaa aagtttgatt
     1321 atcgtcctgc aaaaaaacat ggaaatattc ctttataatt caatatttta tattgaattt
     1381 cacttctgct tcagactttt attagaaagt taacaaaaaa cagaaacgat tatttaattt
     1441 taaaaataaa aaaattagga ataaatggga aaaaatgatt ttcagaaaaa aaataatgca
     1501 aaaaaagatg aaacccctga atttgttgac tttgtagtta cagcaagaaa atataaaaca
     1561 cttttaacta aaaaatatct taatagaaaa aaatgggaaa atcctagtcc ttatgaaatt
     1621 tactcatata ttccaggtac tattgtggag gtttttgtaa aagaaggcga ggtagtagaa
     1681 gaaggagaac cacttttaac attagaggcg atgaaaacac taaatcgtat cgaaatgcct
     1741 tttacagcta aaattaaaaa aattaatgtt aaacctggcg atcaaattcc taaggattat
     1801 ttaatcattg agttagaacc cgttgaagaa taatttttaa aaaatattca gataattcat
     1861 ctctttgtat gatttttaaa ttttcaaaga caaagagatt tttttaaatg aggatttaag
     1921 tgttaattgt cttttttgat taaaagttgt acttttgcaa atgaccaaaa attcaatgag
     1981 aaagagatta aataaaaatg taatttgttt ttataaatag attcttaatt ttttacttat
     2041 ggatcaaact gaacagcaat ttgacaaaat tgtttctatt tgcaaagata ttttcattaa
     2101 aaaattaaaa gattacggtg cttcctggcg ccttttacgt ccttcttcag ttactgacca
     2161 gctttttatc aaagccaatc gtattaggtc tattcaaatg aaaggaattt caaaaatcaa
     2221 cgaagatata aaatctgaat ttattggaat catcaattat ggaatcatcg gtttaattca
     2281 actagaatta ggctttgccg aaaaagtaga tataagtact gaacaagctg ttgaactata
     2341 cgataaatat atccagaaaa gcaaagattt gatgtccaat aaaaatcatg attatgatga
     2401 agcgtggcga agtatgcgag tggagtcatt tgtggatatt attcttatga aaatctgtcg
     2461 tataaaagaa attgaaaatc atgcaggaaa aactttaatt tcagaaggaa tcgatgccaa
     2521 ctattatgat attatcaatt attcaatttt tgctttgata aaactggaag aaatggaaaa
     2581 aacgcacaat aaccattaaa taattgaatt acattttaat aagaatcaca gttgaaaaaa
     2641 taaaaaatct tttgtttttt aagtttattt tatagaaaat gacttacaat tccacgaaaa
     2701 agaaaaaatc aaaatctcat aaagaaacaa aaacggctaa tttttttctt gaattggctc
     2761 gaataatttt gggcttggtt tttatttttt caggctttgt taaagcaatt gatcccttag
     2821 gttcagccta taaaattgaa gattatttaa tagcttttgg tcaccctttt tcttatttca
     2881 tttttctttc tcttcctttt gctattttac tttctacttt tgagttgaca cttggattta
     2941 acttgatatt aaagatttat ccacgtatta ctatcatttt aaccgctatt tttttgacag
     3001 cacttactgc cctcacactt tatctggcac ttaaaaatcc gataactgat tgtggttgtt
     3061 ttggtgatgc aattatattg accaattggc aaacttttta caaaaatctg gtacttatct
     3121 ccttagctgt attgctttta atttaccgaa aaagattcac tcctctgctt ttacctcata
     3181 ttgaaccatt tgcattggta atttttgcct tgttgggaat agttatttca atttattgtt
     3241 atcatcattt accattaatc gattttctac catataaagt aggtgtaaat attccagaag
     3301 ctatgcatat tcctgaaggt gccaaaacag accaatatga aacgactttt atttatgaaa
     3361 aaaatgggca acgaaaagaa tttacgctcg aaaattatcc aaaagatgat tctacgtggg
     3421 tatttatcga tcaaaaaacc actttaaaat caataggtta tcgtcctcct attcatgatt
     3481 ttgttatttt tgatactcaa gatgaagata ttactgatat aattcttcaa gaaaataatt
     3541 ctgcttattt attaattatg tacgatgtaa acaaagcttc aaaagttggt gccaaaaaag
     3601 cagaaaaagt ttatcaaaaa gcacagtcaa caaacagctt tttctgtgct gttacagctt
     3661 cttctgatga agagattcaa aaatttgtcc aagctacggg cgtaacttat cctttttgta
     3721 aggctgatcc cataatgttg aaaacaattg ttcgcgccaa tccagggttg gttttaataa
     3781 aaaatggaac aattatggga aaatggaact ggcgagattt ttagattgaa atttattcat
     3841 tggtgaagaa ttaattaaat ttgacctata tcattgatta tcaaatttat tttataaata
     3901 atttatcata aaatataaaa agatgagaaa aaacattgtc gcaggaaatt ggaaaatgaa
     3961 taaaaccctt ccagaagggt tgaaattagc aactgaattg aatacgcttc tggcaaatgt
     4021 taaattaaat tgtcagataa ttattgctcc tccgttcatt catttagcaa gtattgttgc
     4081 tgccattgat acaaaaaaaa taggagtagc tgctcaaaat tgtgctgata aggaatgggg
     4141 agcttataca ggcgaagtaa gtgctggaat ggtaaaatca actggagcag aatttgtcat
     4201 tttaggacac tcagaacgcc gcacttatta cggggaaaca cctgaaattc tgaaaaccaa
     4261 agtagaattg gcactgaaat atgaattaac gcctattttt tgtattggtg aaatattgag
     4321 cgaaagagaa gcaggaaaac attttgaagt agtcagatca caattggaaa aatcgttgtt
     4381 tcatcttcca gcagaagagt tttcaaaact gattcttgct tatgaaccag tttgggctat
     4441 tggcactggt aaaaatgcta ctcccgaaca agctcaggaa atgcatgctt ttattcgaaa
     4501 aaccattgaa gaaagatatt cttctctggt ggctgataat ctttctattc tttatggtgg
     4561 gagttgtaat ccaggaaatg caaaagaact gtttgcaaat cctgatgtcg atggcggact
     4621 tattggtggg gcttcattga aatcgcaaga ttttaaagcc attattgatg ctttttaaat
     4681 cactgttttt gaaaatattt tatgattatc cgcaaagtat cctactttaa aagggataaa
     4741 ttacagtata tt
//
LOCUS       DAOTKS010000089         4730 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_15732_length_4730_cov_4.422888, whole genome shotgun sequence.
ACCESSION   DAOTKS010000089 DAOTKS010000000
VERSION     DAOTKS010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15732_length_4730_cov_4.422888"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="P5236_02785"
     CDS             <1..442
                     /locus_tag="P5236_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRU63321.1"
                     /translation="ITFTLALCTFLVVNISGTKEYWKGIFWPEVPVWLKVPIPLMPVI
                     EFIEIFTKPFALMIRLFANIVGGHSIVGGLVCLVFVTVKLGTTINVTMSALSVIFTVF
                     IDLIELLIAYIQAYIFTLLSALFIGLARVDPPHKISAVEATEQV"
     gene            520..768
                     /gene="atpE"
                     /locus_tag="P5236_02790"
     CDS             520..768
                     /gene="atpE"
                     /locus_tag="P5236_02790"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HRU63322.1"
                     /translation="MLLSILLQVATLDALAKLGAALAIGLTVIAAGSGIGKIGTSAME
                     SIARQPEMSSDIRMSMIIAAALIEGVSLFAIVVCGFIL"
     gene            836..1336
                     /gene="atpF"
                     /locus_tag="P5236_02795"
     CDS             836..1336
                     /gene="atpF"
                     /locus_tag="P5236_02795"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HRU63323.1"
                     /translation="MSLLTPDGGLVFWMLLSFGIVVLILAKFGFPVILQMIEKRKSYI
                     EESLLEAEKARNELENVKLEGKKIINEAHQAHEKIMKEAAQLKESLLNDAREKASQEA
                     EKILQEARENIQKEKEAALEDIRRQVADMSIVISEKLLRTKLRNKEEQMQIIDRLIDE
                     MKINKS"
     gene            1345..1902
                     /locus_tag="P5236_02800"
     CDS             1345..1902
                     /locus_tag="P5236_02800"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HRU63324.1"
                     /translation="MKTGLIASRYAQALYEFASDYKMEDEIYKGMKILEKSFSLNREL
                     NTVLRNPLLKRELKKQLIVTAYGGEVDIVFERFIELILNNKREAYLQNIVLKYLDVYY
                     EHKNLCIVKLKIAFPIGQESEKKLIDSIKKLVLKDLIVEKEIDPKIIGGFILEIGDVR
                     WDGSLATQLNKIHKKLMEGTFLNLN"
     gene            1960..3549
                     /gene="atpA"
                     /locus_tag="P5236_02805"
     CDS             1960..3549
                     /gene="atpA"
                     /locus_tag="P5236_02805"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRU63325.1"
                     /translation="MPENAIKVSEISELLKKQLEQIDINVQYDEIGEVLEVSDGVARI
                     YGLTNVGANELLEFENGVMAIAMNLEEDNVGAILLGPTYLVKEGFTVKRTGRVASINV
                     SEEMLGRVINPLGVPIDGKGSIGGDFFEMPLERKAPGVIFRQPVSEPLQTGLKAIDAM
                     TPIGRGQRELIIGDRQTGKTSITVDTIINQLDNFKAGNPVYCIYVAIGQKGSTVASLV
                     NTLKEYGAMSYTIVVAANASDPAALRYLAPYAGAAIGEYFRDTGRHALVIYDDLSKQA
                     VAYREVSLILRRPPGREAYPGDIFYLHSRLLERAAKIIAQQEVAEQMNDLPECLKGKV
                     KAGGSLTALPIIETQAGDISSYIPTNVISITDGQIFLDTDLFNEGVRPAINVGISVSR
                     VGGNAQIKPMKKVAGTLKTDQAQFRELEAFTKFGSEMDAVTAMTIDKGQKNTKLLIQP
                     LHKPLSVEKQVAILYCGTHGLLADVPLDKVKDFEDKFLTILEYSYKEEVLNEIKKGNF
                     DDKISSLIEEAAQRAQAQIGT"
     gene            3642..4502
                     /gene="atpG"
                     /locus_tag="P5236_02810"
     CDS             3642..4502
                     /gene="atpG"
                     /locus_tag="P5236_02810"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HRU63326.1"
                     /translation="MPTLKELKNRIQSVRSTQKITSAMKMVSSVKLMKAEKAINSFLP
                     YQQALSEILRNYLSNQIQTFSPYTDIRHVQHVAIVVFASNSSLCGNYNSNMIKRMEQS
                     IVTHRDVRKEDILIFPIGKKVSQACKKKNLKIAGDFEEIAAQPTFENTAPIADTLTDM
                     FLKHEIDRVKVIYLHYVNKSSQIMTADTFLPIEIKYRHGKELRNNYIIEPSVEDIENK
                     LIPLALRYHIYAAALEAQASEYAARTIAMQIATDNADKLLQELSLQYNKFRQESITNQ
                     ILDLIAGSLH"
BASE COUNT         1604 a          694 c          918 g         1514 t
ORIGIN      
        1 aattactttt acattagcac tttgtacttt ccttgtagta aacatatctg ggacaaaaga
       61 atattggaaa ggaatatttt ggccagaagt acctgtctgg ctaaaagttc ctattccttt
      121 aatgccagta atagagttta ttgagatttt caccaagcct tttgctttga tgattcgtct
      181 ttttgctaat attgtaggag ggcattctat cgtaggagga ttggtttgtt tggtgtttgt
      241 tacagtaaaa ttggggacaa ccatcaatgt aactatgtct gctctatcag tcattttcac
      301 cgtatttata gatttgattg aattgttaat cgcttatatt caagcttata tttttacttt
      361 gttatcggcg ttatttattg gattggcaag agtggatcca ccacataaaa tttcagcggt
      421 ggaagccact gaacaagtat aaagttattt gaaaaacaaa atttgtttat catttattaa
      481 taaatttttt aatttatcat ttaaaaataa aaagttatga tgttattatc gattttatta
      541 caagtagcta ctcttgatgc tttggcaaaa ttgggagctg ccttagcaat aggtcttaca
      601 gtcattgcag caggttctgg aattggaaaa attggtacct ctgcaatgga aagtattgca
      661 cgccagcccg aaatgagttc tgatattaga atgagtatga ttattgctgc tgctcttatt
      721 gaaggagttt ctttatttgc aattgtagtt tgtggattta ttctttaaat ttttttctaa
      781 aattaagcac ttcagcgtgt ttaatgttgg tttatttttt taaataaaga gtattatgtc
      841 acttttaact cctgatggtg gtttagtttt ctggatgctt ctttcattcg ggatagtggt
      901 tcttattctc gcaaaatttg gttttcccgt cattcttcaa atgattgaga aacgaaagtc
      961 atacattgaa gaatcattac ttgaagctga gaaagctcgt aatgaattag aaaatgtaaa
     1021 attggaaggt aaaaaaatca ttaatgaagc tcatcaagca catgaaaaaa ttatgaaaga
     1081 agccgctcaa ttaaaagaat cattgttgaa tgatgcacga gaaaaagctt cacaagaagc
     1141 agaaaaaatc ttgcaggaag cacgagagaa cattcaaaag gaaaaggaag ccgctttaga
     1201 ggatattcgc cgacaggtgg ctgatatgtc aatagttata tctgaaaaat tattgagaac
     1261 taaattgagg aacaaagaag aacaaatgca aatcatcgac cgtttgattg acgaaatgaa
     1321 aatcaataaa tcttaaactc taaaatgaaa actggcttaa ttgcttcgcg atatgcacag
     1381 gcattatatg aatttgcttc cgactataaa atggaagatg aaatctataa agggatgaag
     1441 attctggaaa aatcattctc tttaaataga gaattgaata cagttcttag aaatcctttg
     1501 ttaaaaagag agttaaaaaa acaacttatc gttaccgctt atggcggaga agtagatatt
     1561 gtgtttgaaa gatttatcga gttgattctc aataataaaa gagaagctta tttgcaaaac
     1621 attgtgctaa aatacttaga tgtatattat gaacataaaa atttatgtat tgtcaagctg
     1681 aagattgctt ttcccattgg acaggaaagt gaaaagaaat taatagattc aattaaaaaa
     1741 ttagttctta aagatttgat tgttgaaaaa gaaatagatc ccaaaatcat tggaggattt
     1801 attttagaga taggcgatgt aagatgggat ggaagtttag ccacccagtt gaataaaatt
     1861 cataaaaaat tgatggaagg aacgttttta aatttgaatt aattttttcc ttcctttgaa
     1921 taaaaaattt atatcattat aaaatttgga gataaaaata tgcctgagaa tgccataaaa
     1981 gtaagtgaga tatcagagct actgaaaaaa cagcttgaac aaattgatat aaacgttcag
     2041 tacgatgaaa ttggggaagt gcttgaagtg agtgatggag tagcaagaat ctatgggttg
     2101 acgaatgtag gagcgaatga attgcttgaa tttgaaaatg gggtaatggc tattgcaatg
     2161 aatcttgaag aagataatgt gggagcaatt ttgctgggac caacatatct cgtaaaagaa
     2221 ggttttaccg ttaaaagaac aggtagggtt gcttccatca atgtgagcga agaaatgctt
     2281 ggtcgggtaa ttaatccttt aggtgtgccg attgacggga agggaagcat tggaggagat
     2341 ttttttgaaa tgccgttgga gagaaaagcc ccgggcgtta ttttccgtca accagtatca
     2401 gaaccattac agacaggttt gaaggctatt gatgccatga cccctattgg acgtggacaa
     2461 cgtgaattaa ttattggcga ccgtcaaaca ggaaaaacca gtattaccgt tgatacgatt
     2521 attaatcaat tagataattt taaagccggc aaccctgttt actgtattta tgtagccatt
     2581 ggtcaaaaag gttctactgt tgcttcgctg gtaaatacac tgaaagaata tggagccatg
     2641 agttatacaa tcgtagtagc agcaaatgct tctgacccgg ctgccttacg ttatcttgca
     2701 ccttatgcag gagctgctat tggtgaatat tttcgcgaca caggccgtca tgctcttgta
     2761 atttatgacg atttgtccaa acaggctgtc gcttatcgag aagtttcatt gattcttcgt
     2821 cgtccacccg gaagagaagc ttatcctggc gatattttct atttacattc tcgtttgttg
     2881 gaaagagctg ctaaaattat tgcccagcag gaagtagctg aacaaatgaa cgatttgccc
     2941 gaatgcctta aaggaaaagt gaaagcaggc ggttcattaa cagctttgcc tatcatcgaa
     3001 acacaggctg gcgatatttc gtcgtatatt cctacaaacg tgatttccat tacagatgga
     3061 caaatatttt tggatactga tttattcaat gaaggggttc gtccggctat taatgtagga
     3121 atttctgttt ccagagttgg tggtaatgct caaatcaagc ctatgaaaaa agtcgcaggg
     3181 acattgaaaa cagatcaggc tcaatttcgt gaattggaag catttactaa atttggtagc
     3241 gaaatggatg ctgtaacggc tatgactatt gataaaggtc aaaaaaacac aaaacttttg
     3301 attcaacctt tgcataagcc tctttcagta gaaaaacagg ttgctatcct ttattgcggt
     3361 acacatggat tattagccga tgttccatta gataaagtga aagattttga agataaattt
     3421 ttgactattc tggaatactc ttacaaagaa gaggttttga acgaaattaa aaaaggaaat
     3481 tttgatgaca agatttcttc tctcattgag gaagcagctc aaagagcaca agcacaaatt
     3541 ggaacataaa tatattctat cttaaaatat ggcttttgtt tttcacttaa tgaaacgttg
     3601 atttggtaaa ctactaattt tcaaattaaa attttttata cgtgcccact ttaaaggaat
     3661 taaaaaatag gatacagtct gtaagatcta cgcaaaagat aacttcggca atgaaaatgg
     3721 tttcttcggt taagctgatg aaagctgaaa aagccataaa cagtttttta ccttatcaac
     3781 aagctttatc agagatttta cgtaattatt taagtaacca aattcaaact ttttcacctt
     3841 atactgatat tcgacatgtt cagcatgtag ccattgtagt ttttgcttca aattcttcgt
     3901 tgtgtggcaa ttataattcc aatatgatta aacgaatgga gcaatccatt gttactcatc
     3961 gagatgtaag aaaagaagac attttaattt tccctatagg aaagaaagtt tcacaagcat
     4021 gtaaaaaaaa gaatttaaaa attgcaggcg attttgaaga gattgctgct cagcctactt
     4081 ttgaaaatac tgctccaatt gcagacactt tgacagatat gtttctcaaa catgaaattg
     4141 atcgggtgaa agttatttat cttcattatg ttaataaaag ttctcaaata atgactgctg
     4201 acactttctt gcctattgaa attaaataca ggcatgggaa agaattacgc aataattata
     4261 ttattgaacc ttccgttgaa gatattgaaa ataaattaat tccgctggct ttacgctatc
     4321 atatttatgc tgcggcactt gaagcacagg cttcagaata tgcagctcgc actatagcca
     4381 tgcaaattgc tactgataat gccgataaat tattacagga actttctctt caatacaata
     4441 aatttcgtca agaaagtatt acgaatcaaa ttcttgatct cattgcaggt tcattacact
     4501 aaatttttaa aacttccctt caagattata atttgagact tataggtcaa aaagcaggct
     4561 gtaatcttct ggaaggtcga ttatgattat ctttgcgata aatcaaaggt tatgaataaa
     4621 aaaagtgttt ttattgtggt agtagagtaa cgaaaaaaaa tgagtttaag caaggcatcc
     4681 agatgtataa ataccccatt cgtgggaagc agttttgtgg aggaaaccat
//
LOCUS       DAOTKS010000090         4720 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_15769_length_4720_cov_4.072026, whole genome shotgun sequence.
ACCESSION   DAOTKS010000090 DAOTKS010000000
VERSION     DAOTKS010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15769_length_4720_cov_4.072026"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3228)
                     /gene="purL"
                     /locus_tag="P5236_02815"
                     /gene_synonym="purI"
     CDS             complement(<1..3228)
                     /gene="purL"
                     /locus_tag="P5236_02815"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HRU63327.1"
                     /translation="MILFFQTSDNQIYAVQSAVELTKEDFQKLIWLFGEAKLLSETQI
                     EGNFIGPRREMITPWSTNAVEITQNMGITGILRIEKFRQVSFAEKDKDSEVYDPMLER
                     VYSKLDQHIFTIDKTPDPIIYIDDIAAYNEKEGLALSEEEIAYLNEVAKKIGRKLTDS
                     EVFGFSQVNSEHCRHKIFNGKFIIDGKEKEMSLFQLIKKTSKENPNFLISAYKDNVAF
                     NQGPEIQQFSPEKGDQPTFFQVKNISSVLSLKAETHNFPTTVEPFNGAATGTGGEIRD
                     RLGGGKGSLPLAGTAVYMTSYPRNSEETGSNKKSSFENDKEQKISVFSKNWERAIQPR
                     KWLYQTPEQILIKASNGASDFGNKFGQPLICGSLLTFEHEENGKKYGYDKVIMLAGGV
                     GYANRRDALKQKVTAGDKVILMGGDNYRIGMGGGAVSSVETGQYDNAIELNAVQRSNP
                     EMQKRVANVIRALTETERNPIVSIHDHGAGGHLNCFSELLEETGGKINISALPVGDPT
                     LSAKEIIGNESQERMGLLLKEEDIEEARKISERERSPFYVVGEATDNKQLVFYQEDGQ
                     KPIDLKLEYLFGNPPRTVIVDETIEEQFADVEIQKDKILEYLQNVLQVESVACKDWLT
                     NKVDRSVTGKVARQQCLGEIQLPISDVGVMTLDYEGTKGIATSIGHAPLAALVDPQAG
                     SVLAIAEALTNIIWAPLVNSLKSISLSANWMWPSKNPGEDARLYKAVEACSDFACSLG
                     INIPTGKDSLSMTQKYGDEKVFSPGTVIISAAGEVSDVKKTVSQVIVNENDTFIYYID
                     FSKDDFQLGGSVLAQTLNKVGDKVTTVKNAQYFVKAFEIIQQLINEDLILAGHDISEG
                     GMIVTLLEMCFPNVNGGLKVCLDGISEKSIVKILFAENPGILIQIKNKEAVDQILKEN
                     KIRYIHIAQPIAERRLEIQQGKISYAFSINELRDIWFHSSYLLDRKQSSEICAQERYM
                     NYKKQPLEFSFLPSFQGKLSQFGLKKPHSTTGIKAAIIREKGTNSDREMAYALYLAGF
                     DVKDVHMTDLASGRETLEDINMIVFCGGFSNSDVLGSAKGWAGAFL"
     gene            3663..>4720
                     /gene="radA"
                     /locus_tag="P5236_02820"
     CDS             3663..>4720
                     /gene="radA"
                     /locus_tag="P5236_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HRU63328.1"
                     /translation="MAKSKTVFVCQNCGVESPKWIGKCPQCGEWNTYVEETVRKTESS
                     SSTNNHLPHQKPVLLSEVEATEEIRIDTLNKELNRVLGGGLVPGSLVLIGGEPGIGKS
                     TLVLQIILHLKNKKTLYVSGEESIQQIKLRAERLQLHHPSCYLASETALTQIFDDIQE
                     IQPDLVVIDSIQTISSDTIESYAGSVSQVRECALAFLKFCKSTSTPVILIGHINKEGN
                     IAGPKVLEHIVDTVLQFEGDRHYMYRILRPIKNRFGDTSELGIFEMLHNGLREVSNPS
                     ELLLSQNHEGLNGIAIASAMEGIRPFLIETQALVSSAAYGMPQRSCTGFDIRRLNMLL
                     AVLEKRVGFKLSQKDVFL"
BASE COUNT         1478 a          922 c          744 g         1576 t
ORIGIN      
        1 caaaaatgca cctgcccagc cttttgccga acccaacaca tcagaattag aaaatccacc
       61 acaaaataca atcatattta tatcttctaa tgtttcacgt cccgaagcta aatctgtcat
      121 gtgaacatct tttacatcaa aaccagccag ataaagagca tacgccatct cacggtcact
      181 atttgttcct ttttcacgga taatggctgc ttttatgcca gttgttgagt gtggtttttt
      241 taaaccaaat tgagatagtt tcccttgaaa agaaggaaga aaagaaaatt ccaacggttg
      301 ttttttataa ttcatgtaac gctcctgagc acaaatttca ctgctttgtt tccgatcaag
      361 caaatacgat gaatgaaacc aaatatctcg taattcattg atagaaaaag cataagaaat
      421 ttttccttgt tgtatctcca gtcttctttc tgcaataggt tgagctatgt gaatatatct
      481 tattttattt tccttcaata tttggtcaac agcttctttg tttttaatct gaatcaatat
      541 accaggattt tcagcaaata atatttttac tattgatttt tcagaaattc catccaaaca
      601 aactttcaaa cccccattta cgtttgggaa acacatttcc agcaaagtta caatcattcc
      661 tccttcagaa atatcgtgac ctgctaaaat taaatcttca tttatcaatt gttggatgat
      721 ttcaaaagct ttaacaaaat attgagcatt ttttacggtg gtgactttat caccaacttt
      781 gttcagtgtt tgtgccagaa cagaaccgcc taattgaaaa tcatcttttg aaaaatcaat
      841 ataataaata aatgtatcat tttcatttac aatcacttgt gaaacagttt tttttacatc
      901 tgaaacttct ccagcagcag aaataatgac tgtaccaggt gaaaaaactt tttcgtcacc
      961 atatttttgt gtcatcgaaa gactatcttt accggttggg atattaattc ctagcgaaca
     1021 ggcaaaatcg ctgcaagcct ctactgcttt gtaaagtcga gcatcttcac ctggattttt
     1081 actaggccac atccagttag cactcaatga gatacttttc aagctattca ccagcggagc
     1141 ccaaataata ttggtcaaag cttcggcaat tgccaacact gaaccggctt gtggatctac
     1201 caatgcagcc agtggagcat ggccaataga agtagctatt ccttttgttc cttcataatc
     1261 taatgtcatt acaccgacat cacttattgg taattgtatt tctcccagac attgttgtcg
     1321 agctactttt cctgtaacgg aacggtcaac tttattcgtc aaccaatctt tgcaagcaac
     1381 agattcaact tgtaacacgt tttgtaaata ctctaatatc ttatcttttt gaatttctac
     1441 atctgcaaat tgttcttcta ttgtttcgtc tactatcacc gtacgaggtg gattcccaaa
     1501 taaatattcc agttttaaat caataggttt ttgtccatct tcctgataaa aaaccaactg
     1561 tttattatca gtggcttcac ctactacata aaaaggagaa cgttctcttt cagaaatttt
     1621 acgagcttct tcaatatctt cttctttcaa caataaaccc atgcgttctt gactttcgtt
     1681 tccaataatt tcttttgcac ttaatgtagg gtctcccaca ggcaatgcag aaatatttat
     1741 tttcccacct gtttcttcca acaattcaga aaaacaattg agatgcccgc cagcaccatg
     1801 atcgtgaatg gacacaatgg gatttctttc agtttccgtt aaagcacgaa taacgttggc
     1861 aacacgtttt tgcatttcag gattggaacg ttgcacagca tttaattcaa tcgcattatc
     1921 atattgtccc gtttcaacag atgaaacagc accaccaccc atcccaattc gataattatc
     1981 accacccatc aaaataactt tatctccagc cgttactttc tgtttcaatg catcacgacg
     2041 attggcataa cctactccac cagctaacat aattactttg tcgtaaccat attttttacc
     2101 attttcttca tgctcaaaag tgagtaatga gccacaaatc aaaggttgac cgaatttatt
     2161 tccaaaatcg gaagcaccat tcgaagcttt aattaaaatt tgttcaggtg tttgatacaa
     2221 ccatttgcga ggttgaatag ctctttccca atttttagag aaaacggaaa ttttttgttc
     2281 cttatcattt tcaaaagaag attttttatt actccctgtt tcttcgctat tccgtggata
     2341 actggtcata taaaccgcgg ttcctgccag tggcaaacta ccttttcctc cgcctaaccg
     2401 atcacgaatt tctccaccgg ttccagtggc agcaccatta aaaggctcta cagtagtcgg
     2461 aaaattgtgt gtttcagctt ttaaagaaag tactgatgaa atatttttta cttggaaaaa
     2521 agtcggttga tctccttttt caggagaaaa ttgctgaatt tcaggacctt gattaaaagc
     2581 tacattatct ttgtaagccg aaatcaaaaa atttggattt tctttagaag tttttttgat
     2641 aagttgaaac aacgacattt ctttttcttt tccatcgata ataaattttc cattaaaaat
     2701 tttatgacga caatgctcag aatttacttg cgaaaaacca aagacttcac tatctgtcaa
     2761 ctttcgtcca atttttttag caacttcatt tagataagca atttcttctt cactcaaggc
     2821 caatccttct ttttcattat aagcagctat gtcgtcaata taaataatgg gatcaggagt
     2881 tttatcaatg gtaaaaatat gttgatcaag ctttgaataa actctttcca gcataggatc
     2941 ataaacttca ctatctttat ctttttcagc aaaagacact tgtcgaaatt tttcaatccg
     3001 taaaataccc gtgataccca tattttgggt gatttctaca gcatttgtac tccagggcgt
     3061 aatcatttca cgacgaggac caataaaatt cccttcgatt tgtgtttcgg acaataattt
     3121 tgcttcaccg aaaagccaga ttaatttctg aaaatcctct tttgttagtt caacagcaga
     3181 ttgaactgca tatatttgat tatcagaagt ttgaaaaaaa agaatcatat ttttatggtt
     3241 ttttcttgat tgaaatataa cattcaacaa tactttttta tttattttac aaagataatt
     3301 attttcgatt cacggagaaa aattccgttg taccatccaa aaattatttc gcatcagaaa
     3361 attgtttata ttaaaaaacc ttatcataaa ccattaacct tcataacagg gaatgtttcc
     3421 tacagcttac aaccacctta ttaccttatt ttcaaaatca ataaatttta ttttagtgtg
     3481 acgcagccag taggctatgt cgaacgaaaa atttcgagta tatttaaatc caacttagca
     3541 agacacaaaa aagattttca aaaagtttac tttccgtcca gtaaaaaaca ctacttttgt
     3601 aaaaatgatt tattattttt attttagcaa attgttgatt tttaaaagat tattataaat
     3661 tcatggcaaa aagtaaaacg gtttttgttt gtcaaaattg tggggtagaa tctcctaagt
     3721 ggattggtaa atgtccccag tgtggtgaat ggaatactta tgtagaagaa acggttagaa
     3781 aaacagagtc atcttcatct acaaacaatc atttgcctca tcaaaaacct gtattacttt
     3841 ccgaggtaga agccaccgaa gagatacgaa ttgatacgct aaacaaagaa ttgaatcggg
     3901 ttttaggagg cggattggtt cctggctcgt tggtattgat tggcggagaa ccgggaatag
     3961 gaaaatcaac acttgtttta caaatcattc ttcatctaaa aaacaaaaaa actctttatg
     4021 tctcaggcga agaaagcatt caacaaataa aacttcgtgc agagaggctt caacttcatc
     4081 atccttcgtg ttatttggca agcgaaacgg cgttaacgca aatctttgat gacattcaag
     4141 aaattcagcc tgatttggtg gtgattgatt ctatacagac tatttcgagt gacacaatag
     4201 aatcttatgc aggttctgta tcgcaagtca gagagtgtgc tttggccttt cttaaattct
     4261 gtaaatctac ttccacgcct gtgatactaa ttggacatat taataaagaa ggaaatattg
     4321 cgggtccgaa agtgctggaa catattgtag atactgttct gcaatttgaa ggcgaccgac
     4381 attacatgta ccgaattttg cgtcctataa agaatcgttt tggtgatact tcggaattgg
     4441 gaatttttga aatgttgcac aatggtcttc gcgaagttag caatccttct gaattattac
     4501 tttcgcaaaa ccacgaagga ctaaacggta tcgctattgc atcagcaatg gaaggaatcc
     4561 gtcctttcct catcgaaaca caggcgttag tgagttctgc tgcttatggc atgcctcaac
     4621 gttcctgcac aggttttgat atacgtcggt tgaatatgtt gctggcagtg ctggaaaaac
     4681 gggtaggttt taaattatca caaaaagatg tttttctgaa
//
LOCUS       DAOTKS010000091         4702 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_15837_length_4702_cov_3.343017, whole genome shotgun sequence.
ACCESSION   DAOTKS010000091 DAOTKS010000000
VERSION     DAOTKS010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15837_length_4702_cov_3.343017"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="P5236_02825"
     CDS             complement(<1..734)
                     /locus_tag="P5236_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRU63329.1"
                     /translation="MKAIDCFVFYEETKEIKKTIQNLLAEELVNNIYLLTKSSVSSGI
                     KGCINLPITSLLSTETVKKIAVHAKAPYSLIFLKNTMLELGQFALERFYQIANDTQAG
                     LVYADYYQITNGTRSKLPLIDYQLGSVRDDFNFGSVLFYNTECLKKAVSEFSENYQYA
                     GFYDLRLKVSQCAELVHIDEYLYSDVELDIRKSGEKQFDYVDPKNREIQLEMEKACTD
                     HLKKIGAYLKPVFKKISFNEEKFPVEA"
     gene            complement(760..2739)
                     /locus_tag="P5236_02830"
     CDS             complement(760..2739)
                     /locus_tag="P5236_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007753536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HRU63330.1"
                     /translation="MKTNLSSKISMIRIPKRYYQPDDVFDLSRVTRFEKIPTEIFDNE
                     QLGSKKVAEEIAELIRKKQSINENCVLGLTSGRSMLGIYAELIRLHKKENLSFHNVIV
                     FLLFEYYPLSSNLQGAYAQLKELLFDHIDIPEENIFYPDGTMDKSEIIEACAAYEQKI
                     ASYGGIDLQLLGIGQTGNIGFNEPGSQVNSQTRLILLDNVSRKDAADYFGSIDLVPIS
                     AITMGIGTILKAKRIIVVAWGEHKSTVIKNAVEGVMSDMVPASFLQLHSNVKIIIDLY
                     AASELTRISMPWLVTSCEWDSKLIRRAIVWLCLRINKPILKLTNKDYNDNGLSELLAI
                     YGSAYNVNIRIFNDLQHTITGWPGGKPNADDTYRPERAFPYPKRVIIFSPHPDDDVIS
                     MGGTFKRLIDQNHEIHVAYETSGNIAVTDEEVIRFLSFTKGFKDLFDSGNKVLEKKYN
                     ELCNFLLNEKKEGDNDTRDILELKALIRRGEAKAACRYLGLPSKNVHFLNLPFYETGT
                     IKKNSLTETDVNIIKQLLQEVRPHQIYAAADLADPHGTHKICLDAVLAAIDELKEEEW
                     MKDCWIWLYRGAWKEWEIDHIEMAVPLSPEELRNKRNAILRHQSQMENAPYLGDDQRL
                     FWQRSEDRNRATAELYTKLGLASYEAIEAFVKYIP"
     gene            2918..3760
                     /locus_tag="P5236_02835"
     CDS             2918..3760
                     /locus_tag="P5236_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HRU63331.1"
                     /translation="MKLIADSGSTKTHWCIIEDKKILKEIFTKGINPFYQSKEEIENE
                     LRFNLIPSLAEFVIDEVHFYGAGCAFQEQKKLISQIIQLFYPNAYIEIQSDLLGAARS
                     LFANEKGIACILGTGSNSCLYDGKKIVMNVPSLGFILGDEGSGAVLGKKLVADVLKNQ
                     LSQELSTNFLNQYHLNIEIILENVYKKPFPNRYLAQFTPFLKENIQHPLIYNIVYTAF
                     NDFFIRNISQYLSKENVSVAFVGSIAFHFSEILKKVASEKQIVISMIRQHPMEGLITY
                     HSEN"
     gene            3781..4605
                     /gene="murQ"
                     /locus_tag="P5236_02840"
     CDS             3781..4605
                     /gene="murQ"
                     /locus_tag="P5236_02840"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HRU63332.1"
                     /translation="MTFQKITEQNSLHDDLDKKSTRQLLIEMNEEDKKVPFAVEKVIP
                     EIEQLIEEIVKRMKIGGRLFYIGAGTSGRLGVLDASEIPPTFGMPPNLVVGIIAGGDI
                     ALRNAVEKAEDDFEQGWEDLKKNNINQKDTVVGLTASGAAPYVIGALKHARENGIFTA
                     SISCNPDSPVSKEANIKIEVIVGPEFVTGSTRLKAGTAQKLILNMISTITMIKLGRVK
                     GNKMIHMQLTNSKLVDRGTRMLVEALNIDYEQARNILLIHGSVKKAIDAFKMKNKT"
BASE COUNT         1630 a          785 c          699 g         1588 t
ORIGIN      
        1 gcctcaacag gaaatttttc ttcgttaaat gaaatttttt taaatacagg ttttaagtaa
       61 gcacctatct tttttaaatg atcagtacac gccttctcca tttcaagctg tatttctcta
      121 tttttaggat ccacgtaatc aaattgtttt tcaccactct tacgaatatc taactctaca
      181 tcagagtata aatattcatc tatgtgtacc aactcagcgc attgagaaac ttttaacctt
      241 aaatcataga aaccagcata ttgataattt tcagaaaatt cactcacagc tttttttaaa
      301 cattcagtgt tgtaaaacaa gacagaacca aaattaaaat catctcgtac gctacctaat
      361 tggtaatcta tcagtggtaa tttagaacgt gttccattcg taatttgata ataatcagca
      421 taaactaaac ctgcttgcgt atcgttcgca atttgataaa aacgttccaa tgcaaactgc
      481 cctaattcca gcatggtatt tttcaaaaaa attaaagaat aaggtgcttt cgcatgaact
      541 gcaatttttt ttaccgtttc agtgctcaac aaggaagtta ttgggaggtt tatacatcct
      601 tttataccag acgagacaga agattttgtt aaaagataaa tattatttac caattcttct
      661 gctaacaaat tttgaatggt cttttttatt tctttcgttt cttcataaaa aacaaaacaa
      721 tctatagctt tcataattat ttaaaatatt agtatttatt tatggtatat atttaacaaa
      781 agcttctatc gcctcataag aagccaagcc taatttggta tataattcag cagtagcacg
      841 atttctatcc tccgaacgct gccaaaaaag ccgttgatca tcacctaaat aaggagcatt
      901 ctccatttgt gattgatgac gtaaaatagc attgcgtttg tttctcaatt cttcaggact
      961 taaaggaaca gccatttcta tatggtcaat ttcccattct ttccaggcac ctcgatacaa
     1021 ccaaatccag caatccttca tccattcttc ttctttcagt tcatcaatag ctgctaaaac
     1081 agcatcaagg caaattttat gtgtgccatg aggatcagct aagtcggcag ccgcataaat
     1141 ttgatgcgga cgaacttctt gtaaaagttg tttaataata tttacatctg tttcagttaa
     1201 agaatttttt ttaatagtgc ctgtttcata aaaaggtaaa ttcaaaaaat gcacattttt
     1261 cgaaggaagt cctaaatatc tacaagcagc ttttgcttca cctcttcgaa tcaatgcttt
     1321 taattcgaga atatcccttg tgtcattatc cccttctttt ttttcattca ataggaaatt
     1381 acataattcg ttatattttt tttcaagaac tttatttccc gaatcaaata aatctttaaa
     1441 tccttttgta aaagaaagaa aacgaatcac ttcttcatct gttacggcaa tgtttcctga
     1501 agtttcgtat gccacatgaa tttcatgatt ttgatcaatc aggcgtttaa aagtgcctcc
     1561 cattgaaata acatcgtcgt caggatgggg actaaaaata atgacacgtt ttggataagg
     1621 gaaagctctt tcaggacgat aggtatcgtc agcattaggt tttccgccag gccatccggt
     1681 aatagtatgc tgcaagtcgt taaaaatacg aatattaaca ttataagctg agccataaat
     1741 agccagcaat tcacttaacc cattgtcatt ataatcttta ttggtaagtt ttaatatagg
     1801 tttgttaatt cttagacata accacacaat tgcccgacga atcaatttgc tatcccattc
     1861 acaggaagtc accaaccacg gcatgctaat tcgcgtaagt tcactggcag cataaagatc
     1921 aataataatt tttacattgg aatggagttg caaaaatgaa gcaggaacca tatcagacat
     1981 aactccttct acagcatttt taatcacagt tgatttatgc tcaccccaag ctactactat
     2041 aattcttttt gctttcaaaa ttgtacctat ccccattgta atggctgaaa taggaaccaa
     2101 atcgatgctt ccaaaataat ctgctgcatc ttttcttgaa acattatcca acaagataag
     2161 gcgagtttgt gaatttactt gtgaccccgg ttcattaaaa ccaatattcc ctgtttgacc
     2221 aatacctaaa agttgaaggt caattcctcc ataagaagct attttctgtt cataggcagc
     2281 acaagcctca ataatttcag atttatccat tgttccatca ggataaaaaa tattttcttc
     2341 aggaatatca atatgatcaa ataataattc tttcaattga gcataagcac cttgcaaatt
     2401 actggacaag ggataatatt caaacaataa aaaaacaata acattatgaa agctaagatt
     2461 ttctttttta tgtaaacgaa tcaattcagc ataaatgccc aacatagatc ttccactcgt
     2521 taatcctaag acacagtttt cattgattga ctgtttttta cgaataagct ctgcaatctc
     2581 ttcggctact ttttttgaac caagttgttc attatcaaaa atttcagttg gtattttttc
     2641 aaaacgcgtc acacgcgaca aatcaaatac atcatcaggc tgatagtatc tttttggaat
     2701 acgaatcatt gaaatttttg aacttaaatt tgttttcatg tttttaaaaa ttacaaattc
     2761 tttgaatttg atttcaaaat tacaataatt ttctttctta acaaattttt tttgcaatcg
     2821 tttgcgttaa aaatgtaatt ttttgttatt ttttctttga aagctaattt cttttccttt
     2881 actttgtagc aaaattaatt tttgcaatcg ataaaatatg aaattaattg ctgatagtgg
     2941 ttctacaaaa acacattggt gtattattga agataaaaag atattgaaag aaattttcac
     3001 aaagggaatc aatccttttt atcaatcaaa agaagaaatt gaaaatgaat taagatttaa
     3061 tttaattcct tcgcttgcag aatttgttat tgacgaagta catttttatg gagcaggttg
     3121 tgcttttcaa gaacagaaaa aattaatttc tcaaattatt caactctttt atcccaacgc
     3181 ctatattgag atacaaagtg atttattagg agctgcacgt tcattatttg ctaatgaaaa
     3241 aggaatagcc tgcatacttg gcacagggtc aaattcatgc ttgtatgatg gaaaaaaaat
     3301 tgtaatgaat gtgccttccc tgggattcat tttaggagac gaaggaagtg gtgctgtttt
     3361 aggaaaaaag cttgtagcag atgtcttgaa aaatcaactt tcacaagaat taagtaccaa
     3421 ttttttgaat caatatcatt tgaatattga aatcatcctc gaaaacgtct ataaaaaacc
     3481 tttcccaaat agatatttgg ctcaatttac tccttttttg aaagaaaata ttcagcatcc
     3541 gcttatatat aatattgtat atacagcatt taatgatttt tttatccgaa atatttctca
     3601 atatctttca aaagaaaatg tgtcagttgc ttttgttggc tcaattgctt ttcatttttc
     3661 agaaatttta aaaaaagtag cttcggaaaa acaaattgtt atatctatga ttagacaaca
     3721 cccgatggag ggattaatca cttaccattc agaaaattaa ttttttaata aaattcatat
     3781 atgacatttc aaaaaatcac agaacaaaat tctttacatg atgacttgga taaaaaatca
     3841 acaagacaac ttctgatcga aatgaatgaa gaagataaga aagtaccatt tgcagttgag
     3901 aaagtaattc cagaaataga acagttaata gaagaaatcg taaaaagaat gaaaataggt
     3961 ggaagacttt tctatattgg agctggaaca agtggacgtt taggagtgtt ggacgcttct
     4021 gaaattcctc ctactttcgg tatgcctcct aatttagtgg tcggtattat tgccggcggc
     4081 gacattgcat taaggaacgc tgtagaaaaa gctgaagatg attttgaaca aggttgggaa
     4141 gatttgaaaa aaaataatat aaaccaaaaa gatacagttg ttggcttaac agcttctgga
     4201 gcagctcctt atgtaattgg agctttaaaa catgctcggg aaaatggcat ttttactgct
     4261 tctatttctt gcaatccaga ttctcctgtt tcaaaagaag caaatattaa aattgaagtt
     4321 atagtgggac ctgaattcgt aactggaagt acacgactga aagcagggac agctcaaaaa
     4381 cttatattaa acatgatttc tactattacc atgataaaat taggtagagt gaaaggaaac
     4441 aaaatgattc atatgcaact aactaattca aaattagtag atagaggaac tcgtatgtta
     4501 gtagaagcat taaatatcga ttatgaacaa gccagaaaca ttcttttaat acatggttcg
     4561 gtaaaaaaag ccattgatgc ttttaaaatg aaaaataaga cataaaataa aaaatgttaa
     4621 atattttgca aacgtttgta atctattatt ttttttattt atttgcacaa aaattatcac
     4681 tataaataag ttttaataga ga
//
LOCUS       DAOTKS010000092         4687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_15893_length_4687_cov_4.182858, whole genome shotgun sequence.
ACCESSION   DAOTKS010000092 DAOTKS010000000
VERSION     DAOTKS010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15893_length_4687_cov_4.182858"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="P5236_02845"
     CDS             <1..226
                     /locus_tag="P5236_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HRU63333.1"
                     /translation="KRHKGYWISLIPAVFMTCVSVSYICIAPEGFQLPQHLSYLISAI
                     ACVVIIFLFYRFIYLNKGKLLAENQLFNKK"
     gene            408..1535
                     /locus_tag="P5236_02850"
     CDS             408..1535
                     /locus_tag="P5236_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63334.1"
                     /translation="MAITVREVTTKKQLKQFVWFGINLYKNNKYAAPPLFIDDYTNLT
                     KGKNPSLDFCDAAYFLAHDERNKIVGRIAMIINPVANETWNQKNARFGWIDFIDDYNV
                     SKALFDTAVAWAKSKGMKAIHGPLGFTDFDHEGTLIEGFDRLGTLATIYNYPYYPKHI
                     ERYGFVKDVDWLEFLITIPNKFPEKYFKIAELVKEKYHLTPKQFKNRKYLVEKYGEKV
                     FDLLNTCYEDLYGFTKLNDAQKKFYIKLYFSFFRLDTVSIVTDENDEVVALGIAMPNL
                     TKALQKAKGRLFPLGWFYLLRAIQKNDIVDLYLMAVHPRYQNKGVNSIIFAELMPKFA
                     KNGYKFAESNPELETNVRVMNLWENFEYINHKRRRAYRLEI"
     gene            1673..3247
                     /gene="nadB"
                     /locus_tag="P5236_02855"
     CDS             1673..3247
                     /gene="nadB"
                     /locus_tag="P5236_02855"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HRU63335.1"
                     /translation="MTRKFDFLVIGGGIAGISFALKVAPYGKVALLCKTTLEESNTSL
                     AQGGMAAVFYPPDSFEKHIQDTLISGDGLCNIEAVKKVVYNAPEQIKQLIEWGVQFDK
                     TKDGKFDLHREGGHSEFRILHKKDYTGYEIQKKLNERVRNTPNIEIFEHHFAIDILTQ
                     HHLGEIVTHHTPNIECYGAYVLDETNDEIHTFLSKVTLLATGGIGGAYLTSTNPPIAT
                     GDGIAMVHRARGVIKDMEFVQFHPTALYQPGVKPSFLISEAIRGYGAVLRTKDGNEFM
                     YKYDERKELAPRDIVARAIDNEMKLRGDEYVYLDVTHKDPKETKKQFPLIYKRCRELG
                     INITKDYIPVTPTQHYLCGGIAVDLDARSSINRLYAAGECSCTGLHGANRLASNSLIE
                     AIVYADAAAKDAIKQVNSFSFKEGIPDWNDEGTTYPEEMVLITQSVREVEQIMNTYVG
                     IVRSNLRLKRAMTRLEILFRETEDLFDRSVVSRQICELRNIISVSYLIIKQATFRKES
                     RGLHYNIDYPYHADTV"
     gene            3302..3685
                     /locus_tag="P5236_02860"
     CDS             3302..3685
                     /locus_tag="P5236_02860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRU63336.1"
                     /translation="MKQVLLILLGCLFILKIEANSYFQDKSHSLNYPQNVKVDVTSLN
                     WMKLSNDSSENEGPLTGNPVSFSTTSEGIYLTLRNGKYDVQLFALTGQLLWSGNLVQG
                     RFFIPVRQGIYFLRINGKSYKVICK"
     gene            3769..4074
                     /locus_tag="P5236_02865"
     CDS             3769..4074
                     /locus_tag="P5236_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HRU63337.1"
                     /translation="MIKHIVLFQLAEFAEGKTKKENAQFIKTELEKLINVIPQLKKIE
                     VGLNHPEAPQYNYDIALYCEFNSLNDLNIYQEHPAHKEVSEYVGKIKTSRVAVDYEV"
     gene            complement(4096..>4687)
                     /locus_tag="P5236_02870"
     CDS             complement(4096..>4687)
                     /locus_tag="P5236_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRU63338.1"
                     /translation="SWTAPQEKTTVFELKAYIENIFLRLGIKRRQLHVQEFSDDLLSK
                     ALVYLNEKGKKIGIFGIVHSNVLKKFDIEQEVYYADLDWNLLLALIKNHKISFTEIPK
                     FPEVRRDLALLLDKNINFSEIEKIAYETERKLLKRVQLFDVYEGENIAPGKKSYAVSF
                     ILQDETKTLTDDQIDAIMKKLMKNFAEKLGAQLRNK"
BASE COUNT         1609 a          740 c          793 g         1545 t
ORIGIN      
        1 aaaaaggcac aagggttatt ggatttctct tatcccagca gtatttatga cttgtgttag
       61 tgtaagttat atttgtattg cacctgaagg atttcagtta cctcagcatc tttcttatct
      121 tatcagtgca attgcttgtg ttgtgattat atttctcttt tatcgtttta tttaccttaa
      181 taagggaaaa ttattagctg aaaatcaatt atttaataaa aaataattca aaaaaattcg
      241 aaattgaatc tttcccgttg aaaattgtgt aattttacac gttttttaac aaacaaaaag
      301 tactttttta aaagcagcat ttttgtaatt gcttcttttt agtaaggaat gaataaccca
      361 ttctatgaaa aagcaaataa aataatttat taaaaaccta aatggatatg gcgataactg
      421 ttcgagaagt aactactaaa aaacaattaa agcaatttgt atggtttggt atcaatttgt
      481 acaaaaacaa caaatatgct gctcctcctt tgtttatcga tgattatact aatttaacta
      541 aagggaaaaa tccttcatta gatttttgcg atgctgctta ttttttagct cacgatgaga
      601 gaaacaaaat agtaggtaga attgcaatga ttattaatcc tgttgccaat gagacctgga
      661 atcagaaaaa tgcacgtttt ggttggatag attttataga cgattataat gtttccaaag
      721 ctctgtttga tactgctgta gcctgggcaa aatccaaagg gatgaaagct attcatggac
      781 ctcttggttt tactgatttt gatcacgaag gaacattaat tgaaggtttt gaccgtttag
      841 gcacattggc aaccatttat aattatcctt attatcccaa gcatattgaa cgatatggct
      901 ttgtaaaaga tgtagactgg ctggaatttt taattaccat tcccaataaa tttcctgaaa
      961 aatactttaa aattgctgaa cttgttaaag aaaaatatca tcttactccc aaacaattta
     1021 aaaatcgcaa atatctagtt gaaaaatacg gagaaaaagt ttttgactta ctcaacactt
     1081 gttatgaaga tttatatggt tttacaaaat taaacgatgc acaaaagaaa ttctatatca
     1141 aactctattt tagttttttc cgattagata cggtttctat tgttactgac gaaaacgatg
     1201 aagtagtagc attgggaata gctatgccca atttgacaaa ggctcttcag aaagcaaaag
     1261 gaagactctt cccgcttgga tggttttatc tactaagagc aattcaaaaa aacgacattg
     1321 ttgatttata tttaatggct gtacatcctc gttatcaaaa taaaggagtc aattcaataa
     1381 tatttgctga attgatgccg aaatttgcta aaaatggtta taaatttgct gaaagcaatc
     1441 cggaattaga aacaaatgta cgggttatga atctatggga aaattttgaa tatatcaatc
     1501 acaaaagaag aagagcttat agacttgaaa tataaaatct ttcttatgaa atattttttt
     1561 ctttatcaac ccagaaaatt ttgatgaaga ctccacacgt ccactttaac tttaaaaagt
     1621 ttgaatgtgg tgaagcccaa ttataatact tttgatttaa aattaaatta ttatgacacg
     1681 taaattcgat tttttagtaa ttggtggagg aattgccgga atcagctttg ctctcaaagt
     1741 tgctccttat ggaaaagtag ccttgctttg taaaacaaca ttagaagaat ctaatacgtc
     1801 gcttgcccaa ggaggaatgg cggccgtttt ttatcctccc gatagttttg aaaaacatat
     1861 tcaagacact ttaatttctg gagatggact ctgcaatatt gaagctgtga aaaaagttgt
     1921 ttataatgcc cctgaacaaa tcaaacaact gattgaatgg ggtgtacaat tcgataaaac
     1981 aaaagatgga aaatttgatc ttcaccgaga aggtggacat tcagaattca gaatccttca
     2041 caaaaaagat tatacaggtt atgaaattca aaaaaaatta aacgaacggg ttcgaaatac
     2101 tccaaacatt gaaatttttg aacatcattt tgccattgat atacttacac aacatcattt
     2161 aggcgaaata gtgactcatc atactcctaa tattgaatgt tatggtgctt atgtgctgga
     2221 cgaaaccaac gatgaaattc atacgttttt atctaaagtc actttgctgg caacaggagg
     2281 aattggcggt gcctatctta catctaccaa tccacctatt gcaactggtg atggaatagc
     2341 aatggttcat cgtgctcgtg gcgtgataaa agatatggaa tttgttcaat ttcatccaac
     2401 cgccctttat caaccgggtg taaaaccttc ttttttgatc tcggaagcta taagaggtta
     2461 tggtgctgtg ttacgtacta aagatggaaa tgaatttatg tataaatatg acgaaagaaa
     2521 agaactggca ccacgcgata ttgtagcaag agctattgat aacgaaatga aactccgtgg
     2581 ggatgaatat gtttatttag atgtgaccca taaagatcca aaagaaacaa aaaagcagtt
     2641 tccgcttatt tataaacgtt gtcgtgaatt aggtatcaat atcacgaagg attacattcc
     2701 agtaactcct acccaacatt atttatgcgg aggaatcgct gtggatttgg atgcccgttc
     2761 atccatcaat aggctgtatg cagcaggtga atgttcttgc accggtttgc acggagccaa
     2821 tcggctggct tccaactcat tgattgaagc cattgtatat gcagatgccg ctgccaaaga
     2881 tgcaataaaa caagtgaatt ctttttcgtt taaagaaggt attcccgact ggaacgacga
     2941 gggaacaact tatcctgaag aaatggtgct gatcacacaa agtgtcagag aagtggaaca
     3001 aattatgaat acttatgtag gaattgttcg ttccaattta cgtttgaaga gagccatgac
     3061 tcggctggaa attttgttcc gtgaaacaga agacttattt gaccgctcag ttgtttcacg
     3121 tcaaatttgc gaactaagaa atattattag tgtctcttat cttattataa aacaagcaac
     3181 tttccgaaaa gagagccgcg gtttgcatta caacattgat tatccatatc atgccgatac
     3241 cgtgtgaaca actcgtctct ttcttttgtt attaaaatag caaaaagaat agtattacag
     3301 aatgaaacaa gtgttgttaa tattattggg atgtttattt attctaaaaa tagaagcaaa
     3361 ctcctatttt caggacaaat ctcattcact gaactatcct caaaatgtga aagttgatgt
     3421 tacttctctc aattggatga aattaagtaa tgattccagc gaaaacgaag gtcctctaac
     3481 aggaaatcct gtaagttttt caactacatc tgaaggaatt tatcttactt tgagaaatgg
     3541 aaagtacgac gttcagttgt ttgctctgac aggacaatta ttatggagtg gcaatcttgt
     3601 tcaaggacga ttttttattc cagttcgtca aggaatttat tttttacgta tcaatgggaa
     3661 aagttataaa gtaatatgca aataattagc attttaaatt taataaggaa taattcaaaa
     3721 taagtattta gcttaaaaaa attgatctat ctaaactttt aaatttttat gataaagcac
     3781 attgttcttt ttcaactggc agaatttgct gaaggaaaaa cgaagaaaga aaatgcccag
     3841 tttatcaaaa ccgaattaga aaaacttatt aatgtaattc ctcagctcaa aaaaattgaa
     3901 gtggggctca atcatcccga agcacctcaa tataattatg atattgctct atattgcgaa
     3961 tttaattcac tgaacgattt aaatatttat caggaacatc cggcacataa agaagtttct
     4021 gaatatgtcg gaaaaataaa aacgagtcgt gtagctgtgg attatgaagt gtaaattaag
     4081 caattaaaaa attaatcatt tgttccgcaa ttgagctcct aatttttcag caaaattttt
     4141 cattagcttt ttcataatgg catcaatttg atcatcagtt aaagttttcg tttcatcttg
     4201 caaaataaaa cttactgcat aggatttttt accgggagca atattttccc cttcataaac
     4261 atcaaacaat tgaactcttt tcaatagctt tcgttccgtt tcataagcaa ttttctctat
     4321 ttcagaaaaa tttatatttt tgtcaagcag taatgccaaa tcacgacgaa cttcaggaaa
     4381 tttgggaatc tctgtaaaac ttattttgtg attttttatt aatgctaaaa gtaagttcca
     4441 gtccaaatca gcataataaa cttcttgctc tatatcgaat ttttttaaaa cattagaatg
     4501 tacaatgcca aaaattccga tttttttccc tttttcattt agataaacta aggctttgct
     4561 tagcaaatcg tccgaaaatt cctgaacatg aagttgacga cgttttattc ctaatcgcaa
     4621 gaaaatattt tctatataag cttttagttc aaaaaccgtg gttttttctt gtggtgctgt
     4681 ccatgat
//
LOCUS       DAOTKS010000093         4611 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_16183_length_4611_cov_4.211150, whole genome shotgun sequence.
ACCESSION   DAOTKS010000093 DAOTKS010000000
VERSION     DAOTKS010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16183_length_4611_cov_4.211150"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="P5236_02875"
     CDS             <1..567
                     /locus_tag="P5236_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63339.1"
                     /translation="ISNFIDLDKALSTDATFILQIDDKNQIALSWWVSAKRTRSYPYA
                     RVYDSLSFAGKKITIIPIVKDEGKEGDRDFLQWDTISLMSLLGVYVIIAYYNDATQSS
                     RYDKKITEQRFDINYLKSQINKLLSYQSDALHWNLSQIDNIAEIGKKALDSYDKISQK
                     VGVEMHSKKSAEKRIMELKMAPSNENRL"
     gene            complement(474..803)
                     /locus_tag="P5236_02880"
     CDS             complement(474..803)
                     /locus_tag="P5236_02880"
                     /inference="COORDINATES: protein motif:HMM:NF013695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HRU63340.1"
                     /translation="MSHHEHQDYQKHNDIKNIKTAFFLNFGFTIFEILGGIFTNSIAI
                     VSDAVHDLGDSLSLALAWYFQKKSKKSSDKSYSYGYKRFSLLGAIFNSIILFSADFLE
                     CISTPTF"
     gene            995..3694
                     /locus_tag="P5236_02885"
     CDS             995..3694
                     /locus_tag="P5236_02885"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HRU63341.1"
                     /translation="MKTSKWRKLWIILAVIALFFIILLCLPFVFKGKIVDFTKQQLNK
                     QLIAHIDFDKIQLSFIRRFPNASIQIKNLKIIGIDEFEKDTLLATEKIEAVVNLKSFF
                     ADSGYDISKVLLANSQIKAHILPDGKANWNIVKETPKTEKDTSKMNFHLKLKDVILQN
                     INLTYLDEQGNMKIQVCNLNHHTSGDLTADSSLLKTDTRIDSLSFWMNNVAFLSKANV
                     NLKADVDADLNNMMFKLSKNSILINALPFSINGWLKVLDDGFDMDLKLLADKVDFKSI
                     LSLIPALYYQSFKELKADGKISLDASVKGQMKEDNYPSFNLKLNVDDGWFRYPSLPKA
                     VQNIQIATQIFHPDGNLDKTVVDISKVSFVLDKQPFFVSCRILTPVSDPQIQAVAQGK
                     IEMDKIKDFYPLGDKTKLNGLLDVDASLNGRMSYIENNQYDKFNFQGKLNLERFLMKD
                     VNLYPQEITINNASLLMNPLFVNLSLSGLKIGENNLSAQGKLENLIAYLLKNKTLSGQ
                     LNAQSSYLNLNDFITSQPDNESATEKKVLEIPKNINFIVNADLNKVQFDKMIFENIKG
                     KLEIENGELKLQNVALQAFGGNILAKGLYSTLLPENPKLKVDLNIDSVVINKIFTQIG
                     FMEKLVPFLKNTDGKFSTNLSLGANLKNDMSLDLTSLNANGVFSTRAVGIKNSPIFNA
                     LANNLNHPEFMQPTLKDIALKFEILNGKIFIKPFDFKISNIKMNLGGSSSLNKEIDFQ
                     GIIQLPDQMNIGRFSTVKVNITGTYDKPKIKLDVMNTLSEIVDETKLKVEAEVAKQVD
                     KAKQEALEKAQKQKEKALQEAQQQADKIRLEAQKKSEKILEEARKEGDALVAKATNPV
                     AKKVAETTAKKLLDEAQKKVDQINSEAEAEAQKIIQNATKNLQ"
     gene            3746..>4611
                     /locus_tag="P5236_02890"
     CDS             3746..>4611
                     /locus_tag="P5236_02890"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRU63342.1"
                     /translation="MMINLQKKYRLFSILLLLVSFSVLGQTPQEADNLFQQKKYLEAK
                     KAYETLLRRNPKSALYNYRYARCCYELNDDENAITYFERAGTKYPLRNKYLGELYMKA
                     YRFSESANAYQGYLTTLKENDADYDKIKLALKRAQLAENFIQRVDDISIIDSMIVDKS
                     KFLNFYNFTHELGSLSASPIQLKNRAEEKIVYTTERQDRMYFSDSIRGQMDIFTCYKL
                     LEKWSNPKPVSNQINTSANENYPFLMLDGLTLYFASDGENSIGGYDIFITRYVPSEND
                     FFIPDNIGMPYNS"
BASE COUNT         1740 a          621 c          738 g         1512 t
ORIGIN      
        1 atttctaatt ttattgattt ggataaagca ttatcaactg atgcgacatt tattttacag
       61 attgatgata aaaaccagat tgctctaagt tggtgggtgt ctgcaaagcg aacaagatct
      121 tatccttatg caagagtata cgactctctt tcttttgcag gaaagaaaat aacaataatt
      181 cctatcgtaa aagatgaagg aaaagaaggc gatagagatt ttctgcaatg ggatacaatt
      241 tcattgatga gcttacttgg tgtatatgtc ataattgctt attataatga tgctacgcaa
      301 agctcaagat atgataaaaa gataactgaa caaagatttg atattaatta tctcaaaagt
      361 cagataaata aacttctttc atatcagtca gatgctttac attggaactt atcgcaaata
      421 gataatattg ccgaaatagg taaaaaggcg ttggactctt atgataaaat ttctcaaaaa
      481 gtaggcgtag agatgcattc caaaaaatca gcagaaaaaa gaataatgga attgaaaatg
      541 gcaccaagca acgaaaatcg tttgtaaccg tatgaataag atttgtcgct gctttttttc
      601 gatttctttt gaaaatacca tgccaatgcc agcgaaagac tgtcgcctaa atcatgtacg
      661 gcatcagaaa caatggcaat actgttagta aaaatacctc ccaatatttc aaaaatggta
      721 aatccaaaat taagaaaaaa agctgtctta atatttttta tatcgttatg tttttgataa
      781 tcttgatgtt cgtgatgcga catgattagt tatgtttttg gaaaaatgat tacgaaaaaa
      841 cttttaaaat ttagttaaaa tgtttacttt tttaaaatac aaaggtatgt tttttgatta
      901 aattgattga aaaagcatca cttaattatt ctattttttg ttaaattaat cctatctttg
      961 aaaaataaat caatcatttt aaattcagaa aaatatgaaa acatctaaat ggcgtaaact
     1021 gtggattatt ttagcagtta ttgcattgtt ttttataata ttattgtgtt tgccttttgt
     1081 ttttaaaggt aaaattgttg attttacaaa gcaacaatta aacaagcaat taattgctca
     1141 tattgatttc gataaaattc agctaagttt catacgtcgt ttccccaatg cttctataca
     1201 aattaaaaac ttgaaaatca ttggaataga tgaatttgaa aaggatactt tattggctac
     1261 tgaaaaaatt gaagcggtag tgaatttgaa aagtttcttt gctgatagtg gctacgatat
     1321 ttcaaaagtt ttgctggcaa actctcaaat aaaagctcat atattgcccg atggaaaagc
     1381 aaattggaat attgtgaagg agacaccgaa aactgaaaaa gatacttcaa aaatgaattt
     1441 tcatttaaaa ttaaaagatg tcattttgca aaatataaat ttgacttatc ttgatgaaca
     1501 aggaaacatg aaaattcaag tttgcaattt gaaccatcat accagtggcg atttgactgc
     1561 tgacagctca ttgctaaaaa ccgataccag aattgattct ttgagttttt ggatgaacaa
     1621 tgtggcattt ctttcaaaag ctaatgttaa tttaaaagca gatgttgatg ccgatttaaa
     1681 taacatgatg tttaaattga gtaaaaattc aatcctcatc aatgctcttc ctttttctat
     1741 aaatggttgg ttaaaagttt tggatgatgg atttgatatg gatttaaaat tgctggccga
     1801 taaagttgat tttaaatcaa ttttgtcatt gataccagct ttgtattatc aatcattcaa
     1861 agaattaaaa gctgatggta aaatttctct cgatgcctca gtaaaagggc aaatgaaaga
     1921 agataattat ccttctttta acttaaaatt aaacgtagat gatggctggt ttcgttatcc
     1981 ttctttacct aaagctgttc agaatatcca aatagctact caaatattcc atcctgatgg
     2041 taatttagat aaaacagtgg tggatatttc taaggtttca tttgtattag ataaacaacc
     2101 cttttttgtt tcgtgccgaa ttttaacccc tgtttctgat ccacaaattc aggcagttgc
     2161 tcaaggaaaa attgaaatgg ataaaattaa agatttttat ccacttggtg ataaaaccaa
     2221 attgaatgga cttcttgatg tagatgctag tttaaatgga cgaatgtcgt atattgaaaa
     2281 taatcaatat gataaattta attttcaagg gaaactaaat ttggaaaggt ttttaatgaa
     2341 agatgtaaat ctttatccac aggaaatcac cattaataat gcgagtttat tgatgaatcc
     2401 tttatttgta aatttatcat tatcagggct aaaaataggt gaaaacaatt tatcagctca
     2461 aggaaagctt gaaaacctga tagcttactt attaaaaaac aaaactttaa gtggtcaatt
     2521 aaatgcacag tcttcttatc tcaatttaaa tgatttcatt acatcacagc ccgataacga
     2581 aagtgcaaca gaaaagaaag ttttggaaat tcccaaaaat ataaatttta ttgtaaatgc
     2641 tgacttaaat aaagtgcagt ttgataaaat gatttttgaa aacatcaaag gaaaattaga
     2701 aattgaaaat ggagaattga aacttcaaaa tgttgcattg caggcttttg gtggaaatat
     2761 cttagcaaaa ggtttgtaca gtactttatt gccagaaaat ccaaaattaa aagtagattt
     2821 aaacattgat agtgttgtta ttaataaaat ttttactcaa ataggattta tggaaaaact
     2881 ggttccattt ttgaaaaata ctgatggaaa attttcaacg aatctttcgc ttggtgcaaa
     2941 tttaaagaat gatatgagtt tagatttaac ttcattaaat gcgaatggtg ttttttcaac
     3001 cagagcagtt ggaataaaaa attctcctat cttcaatgct ttggctaata atttgaatca
     3061 tcctgaattt atgcaaccaa ctttaaaaga tattgcctta aaatttgaaa tattgaatgg
     3121 taaaatcttt ataaaacctt ttgattttaa aatttcaaat ataaaaatga atttaggcgg
     3181 tagttcaagc ttaaataaag aaattgattt tcagggaatt atccaattac ctgatcaaat
     3241 gaacataggg cgtttttcta ctgtaaaagt caacattaca ggtacttatg ataaaccaaa
     3301 aattaagttg gatgtcatga atactttaag tgaaatagta gatgaaacaa aattaaaagt
     3361 agaagccgaa gtagcaaaac aagtagataa agccaaacaa gaagcattag aaaaggctca
     3421 aaaacaaaaa gaaaaagctt tacaggaagc ccaacaacaa gctgataaaa ttcgattaga
     3481 ggctcagaaa aaaagtgaaa aaattcttga agaggctcgt aaagaaggcg atgcccttgt
     3541 tgcaaaggct acaaatcctg tggctaaaaa agtagctgaa actactgcaa aaaaattatt
     3601 ggacgaagcc cagaaaaaag ttgatcaaat aaacagtgaa gcagaagctg aagcccaaaa
     3661 aattatccaa aatgcgacaa aaaatttgca ataaatccat ttttaactga attcgttttt
     3721 aaaatagttg ttaaacgatt tttaaatgat gattaactta caaaaaaaat accgattatt
     3781 ttctatatta ttattacttg tttccttttc agtattggga caaacacctc aggaggcaga
     3841 taatttgttt cagcaaaaaa aatatctcga agcaaaaaaa gcttatgaaa ctttgttacg
     3901 acgaaatcca aaaagtgctt tatataatta tcgttatgca cgttgctgtt atgaactaaa
     3961 tgatgatgaa aatgctatca catattttga acgtgctgga acaaaatatc ctttacgaaa
     4021 caaatattta ggtgaattgt acatgaaagc ttatcgtttt tcagaatctg caaatgctta
     4081 tcaaggatac cttactacct taaaagaaaa tgatgcagat tatgataaaa tcaaacttgc
     4141 attgaaaaga gcacaattgg ctgaaaattt tattcaaaga gtagatgata tttcaataat
     4201 agatagtatg atagtagata aaagtaaatt tctaaatttt tataatttta ctcatgaatt
     4261 aggttccctt agtgccagtc ccattcaatt aaaaaatcga gcggaagaaa aaatagttta
     4321 tactactgaa cgtcaagaca gaatgtattt ttctgattct attcgtgggc aaatggatat
     4381 ttttacttgt tacaaattat tagaaaagtg gtctaatcct aagcctgttt ccaatcaaat
     4441 taatacttca gccaatgaaa attatccttt cctcatgttg gatggactta cattgtattt
     4501 tgcatctgat ggtgaaaatt ctataggagg atatgacatt tttattaccc gttatgttcc
     4561 atcagaaaac gattttttca ttcctgacaa tatcggaatg ccttacaatt c
//
LOCUS       DAOTKS010000094         4594 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_16261_length_4594_cov_4.752809, whole genome shotgun sequence.
ACCESSION   DAOTKS010000094 DAOTKS010000000
VERSION     DAOTKS010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16261_length_4594_cov_4.752809"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..643
                     /gene="rplD"
                     /locus_tag="P5236_02895"
     CDS             11..643
                     /gene="rplD"
                     /locus_tag="P5236_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRU63343.1"
                     /translation="MELSVLNIKGEDTGRKVSLNEKIFSIEPNDHAIYLDVKQHLANR
                     RQGTHKTKVRSEVTGSTRKLGRQKGGGGARPGDIKSPLRIGGGTIFGPVPRSYSFKLN
                     KKLKQLARKSALSYKAKDQQITVVEDFDFDTPKTKKLIEIIKNLQVADKKPLIVLENA
                     KKNVYLSARNIPAVKVVTVSELNTYILLNAKMLVLTENSISAIEKTFNLE"
     gene            651..944
                     /gene="rplW"
                     /locus_tag="P5236_02900"
     CDS             651..944
                     /gene="rplW"
                     /locus_tag="P5236_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006283655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRU63344.1"
                     /translation="MENIIIKPLVTEKLTKQTEKFNRYGFKVNRDANKIQIKEAVEAL
                     YNVTVIKVNTMIVAPKKRSRYTKSGIIEGEKSAYKKAIVTLKKGEEIDFFSNI"
     gene            954..1778
                     /gene="rplB"
                     /locus_tag="P5236_02905"
     CDS             954..1778
                     /gene="rplB"
                     /locus_tag="P5236_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRU63345.1"
                     /translation="MAVRKLVPTTPGQRNKIISTFDTITAKEPEKSLVIGKKKTGGRN
                     NGGRRTMRYIGGGHKRKFRIIDFKRNKDGVPATVKAIEYDPNRSAHIALLYYADGEKS
                     YILAPEGLKVGQKVQSGPEAEPEVGNALPLANIPLGTIIHNIELRPGQGASMARSAGT
                     FAQLISREDKYVIVKLPSGEIRKILSTCKATIGSVGNSDHALIKSGKAGRSRWLGRRP
                     RVRGVAMNPVDHPMGGGEGRASGGHPRSRKGLLAKGYKTRYPKKESNQYIIERRKK"
     gene            1808..2071
                     /gene="rpsS"
                     /locus_tag="P5236_02910"
     CDS             1808..2071
                     /gene="rpsS"
                     /locus_tag="P5236_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRU63346.1"
                     /translation="MSRSLKKGPYINLKLEKKILAMNESGKKSVIKTWARASMISPDF
                     VGHTIAIHNGNKFIPVYITENMVGHKLGEFALTRTFRGHSGKK"
     gene            2116..2529
                     /gene="rplV"
                     /locus_tag="P5236_02915"
     CDS             2116..2529
                     /gene="rplV"
                     /locus_tag="P5236_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRU63347.1"
                     /translation="MGSRKRISAENRKEEKRTLYYAKLNNVPTSTRKMRLVADMIRGM
                     EVNKALSVLKFSSKEAARKMEKLLKSAISNWENKTEQRADNANLYISKIYVDGGTMLK
                     RLRTAPQGRGYRIRKRSNHVTLFLESRNTNDINAK"
     gene            2547..3194
                     /gene="rpsC"
                     /locus_tag="P5236_02920"
                     /pseudo
     CDS             2547..3194
                     /gene="rpsC"
                     /locus_tag="P5236_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656540.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
     gene            3322..3744
                     /gene="rplP"
                     /locus_tag="P5236_02925"
     CDS             3322..3744
                     /gene="rplP"
                     /locus_tag="P5236_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRU63348.1"
                     /translation="MLQPKKTKYRRQQKGRMKGNAQRGNQLAFGSFGIKSLESKWLTG
                     QQIEAARVAVTRHMERQGQVWIRVFPDKPITKKPAEVRMGKGKGNPEGFVAPVTPGRI
                     LIEIDGVPLDVAKEALRLGAQKLPVTTKFVIRRDYEAI"
     gene            3760..3954
                     /gene="rpmC"
                     /locus_tag="P5236_02930"
     CDS             3760..3954
                     /gene="rpmC"
                     /locus_tag="P5236_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007134553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRU63349.1"
                     /translation="MKSREIRELSNKELVDRMELEKERLIRLKLNHAISPLDNPMEIK
                     KTRRTIARIATELRQREINQ"
     gene            3969..4226
                     /gene="rpsQ"
                     /locus_tag="P5236_02935"
     CDS             3969..4226
                     /gene="rpsQ"
                     /locus_tag="P5236_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRU63350.1"
                     /translation="MEERNLRKERIGVVTSNKMDKTITVTVKWKEKHPIYGKFINKKK
                     KFYAQDDENKCNIGDTVRIMETRPLSKLKRWRLVEIIERAK"
     gene            4229..4594
                     /gene="rplN"
                     /locus_tag="P5236_02940"
     CDS             4229..4594
                     /gene="rplN"
                     /locus_tag="P5236_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRU63351.1"
                     /translation="MIQQETRLTVADNTGAKEALCIRVLGGSKKRYATVGDTIVVTVK
                     SVIPSSDIKKGMVSKAVVVRTKKEIRRPDGSYIRFDDNACVLLNNAGELRGSRIFGPV
                     ARELRATNMKIISLAPEVL"
BASE COUNT         1755 a          655 c          944 g         1240 t
ORIGIN      
        1 ataaataatc atggaattaa gtgtattaaa tattaaagga gaagacacag gtcgaaaagt
       61 gtcattgaat gaaaagattt tcagtattga gccaaatgac catgctattt atttggatgt
      121 gaaacaacat ttggctaatc gtcgccaggg aacccataaa acaaaagtta gaagtgaagt
      181 tacgggaagt acacgtaaat taggcagaca aaaaggcgga ggaggagcac gaccgggtga
      241 tattaaatca ccacttcgta ttggaggggg tacaattttt ggacctgtac ctcgcagtta
      301 cagttttaag ttaaataaaa agttaaaaca attagcacga aaatcagctc tttcttataa
      361 ggctaaagat cagcaaatta cagttgtaga agattttgat ttcgataccc caaaaacaaa
      421 aaaattaata gaaatcatta aaaatttaca agtagctgat aaaaaacctt taattgtttt
      481 agaaaatgca aagaaaaacg tatatttgtc ggctcgaaat attcccgcag taaaagtagt
      541 aacggtttct gaattaaata cttatatttt actgaatgct aaaatgttag tattaacaga
      601 aaattcaatt tctgcgattg agaaaacttt taatttagaa taaaaggaaa atggaaaata
      661 ttattataaa acctctggtt acagaaaagt taacgaaaca aactgaaaaa tttaatcggt
      721 atggatttaa agtaaacagg gatgcgaata aaatccagat aaaagaagca gtagaagctt
      781 tgtataatgt aacagtaatt aaagtaaata caatgatagt agctcctaaa aaaagaagtc
      841 gttacactaa aagtggaatt atcgaaggag agaagtcggc ttataaaaaa gctattgtaa
      901 ccttaaaaaa gggtgaagaa attgattttt ttagcaacat ataaaaataa aacatggcag
      961 ttcgtaaatt agtccctact acacccgggc agagaaataa aattataagt acctttgata
     1021 ctattactgc aaaagagcct gaaaaatctc ttgttattgg aaaaaagaaa acaggtggtc
     1081 gcaataatgg tgggcgaagg acaatgcgct atatcggtgg tggtcataaa agaaaattca
     1141 gaattattga ttttaaacgt aataaagatg gcgtgccagc aaccgttaaa gctattgaat
     1201 atgatccaaa tcgttcagct catattgctt tgctatatta tgctgatgga gaaaaatcat
     1261 acattcttgc tccagaaggt ttaaaagtag gtcaaaaagt tcaatccgga ccagaagctg
     1321 aaccagaagt tgggaacgca cttccattag caaatatccc acttggtact attattcata
     1381 atattgaatt acgtcccggt caaggtgctt caatggcacg ttcagcaggg acttttgctc
     1441 aattaatctc tcgtgaagat aaatatgtta ttgtaaaatt accttcgggt gaaatccgaa
     1501 aaattctttc tacctgtaag gctactattg gaagcgtagg aaattccgat catgctctga
     1561 taaaatcggg aaaagctgga cgttcacgtt ggttgggtag aagaccacga gttagaggag
     1621 tggcaatgaa tcctgttgat caccccatgg gtggaggaga aggacgtgcc tcaggaggac
     1681 atcctcgttc tcgtaaagga ttgttggcaa aaggatataa aacacgttac ccaaagaagg
     1741 aatcaaatca atatattatt gaaagaagaa aaaaataata ataattggat aaaaatcgat
     1801 ataaaatatg agtcgttcat taaaaaaagg accttacatt aatttaaagc tggagaaaaa
     1861 aatccttgca atgaatgaaa gcgggaaaaa atctgtaatt aaaacatggg cacgtgcttc
     1921 aatgatttcc cccgactttg tgggccatac tattgccata cataatggga ataaatttat
     1981 tcctgtctat attactgaaa atatggtagg acataagctt ggcgaatttg ctttgacacg
     2041 aacttttaga ggtcattcag ggaaaaaata agtaaattat tgaaggtcaa ctattaaact
     2101 taaaattttt tagaaatggg ttcaaggaaa agaatatcag ccgaaaatag gaaagaagaa
     2161 aaaagaacac tttattatgc taagctaaat aatgtaccta cttcaactcg caaaatgcgg
     2221 cttgttgccg atatgataag aggaatggaa gtaaataagg ctttaagtgt gttgaaattc
     2281 tcctctaaag aagctgcccg taaaatggaa aagctattga aatctgccat tagtaattgg
     2341 gaaaataaaa ctgagcagag ggctgataat gctaatttat atattagtaa aatatatgtt
     2401 gacggcggca ctatgctaaa acgcttgaga acggcaccgc aaggaagagg ttatcgaatt
     2461 cggaaacgtt ctaatcatgt gacattattt ttagaatcaa gaaatacaaa cgatattaac
     2521 gcaaaataac caaatttaat tactcgatgg gacaaaaaac aaatccaata agcaaccgat
     2581 taggaatcat cagaggatgg gattctaatt ggtatggggg taaaaattac agtgatactt
     2641 tgcttgaaga cagtaaaata agaaaatact tgaatgttcg tttggcaaaa gcaagtgtat
     2701 cacgaattat tattgaacgt actcttaaac ttataaccat tacagtttgt actgcaaggc
     2761 caggacttat tattggtaaa ggtggtcagg aagtagacaa gttgaaagaa gagttaaaaa
     2821 agattacaga taaagaagtc caaattaata tttatgaaat taaacgtcct gaattggatg
     2881 ctgtaattgt agcaaacaat atcgctcatc agattgaagg taaaattgct taccgtagag
     2941 ctgtaaaaat ggccatagct tctaccatga gaatgggagc agaaggaata aaagttaatg
     3001 ttgccggtcg tctgaatggc gttgagatgg cacgttctga aatgtataag gaaggcagaa
     3061 ctcctcttca tacttttaga gctgatatag attatgcttg tgctgaagct ttaacaaagg
     3121 tagggatgat aggagtgaaa gtatggattt gcagaggtga aatttatggc aaaaaggact
     3181 tgactccttc ttatgctatt ggaagtaagg aaattgtaaa aggtgaccat cgcccaggtg
     3241 atcgtcattc aaataatcat agcagaggtt caaaaagaaa aaaaaaataa ataatcttat
     3301 aagaatttga tttaaaccat catgttacaa ccgaagaaaa ctaaatacag aagacagcaa
     3361 aaaggacgca tgaaaggaaa cgctcagcgg ggcaatcaat tagcttttgg tagttttgga
     3421 atcaaatcat tggaatctaa atggttgaca ggccagcaaa ttgaagccgc tcgtgttgct
     3481 gtaacacgac atatggaacg tcaaggacag gtttggattc gtgttttccc ggataaaccc
     3541 attacaaaaa aaccagcaga agttcgtatg ggaaaaggta aaggaaatcc cgaaggattt
     3601 gttgctcctg ttactccagg aagaatactt atagaaattg atggtgtacc tttagatgtt
     3661 gctaaagaag ctcttcgttt aggtgcacag aaattgcctg taacaacaaa gtttgtcata
     3721 cggcgtgatt atgaagcaat ttaaaaatgc atagcaaaaa tgaaatcaag agaaattaga
     3781 gagttatcaa ataaagaact cgtcgatcga atggagttag aaaaagaacg tttaatacgc
     3841 ttaaagttga atcatgctat tagtcctctc gataatccaa tggagattaa aaaaacacgt
     3901 agaactatcg cacgtattgc aactgaatta cgtcagcggg aaattaatca ataaattttt
     3961 attatctgat ggaagagaga aatttaagaa aagaacgcat aggagttgtt accagtaata
     4021 aaatggataa aactattacg gtaaccgtaa aatggaaaga aaaacatcct atttacggta
     4081 aatttataaa taaaaagaaa aaattctatg ctcaggacga tgaaaacaaa tgtaatattg
     4141 gtgatacagt gcgtattatg gaaactcgtc ctttgagtaa attaaaaaga tggcgattag
     4201 tagaaattat tgaaagagct aaatagttat gatacagcaa gaaacaagac taacagtagc
     4261 tgataataca ggagcaaaag aggctctttg tattcgtgta ttaggcggaa gtaaaaaaag
     4321 atatgccact gtgggtgaca caatagttgt aacggttaaa agtgtaatac cttcttcaga
     4381 tattaaaaag ggaatggtgt caaaggcagt ggtagtacgt actaagaaag aaattcgccg
     4441 tcccgatggt tcttacattc gctttgatga taacgcttgt gtattactaa ataatgcagg
     4501 agaacttaga ggcagtcgta tttttggacc tgtggctcgt gaattacgtg ctactaatat
     4561 gaaaatcata tctcttgcac cagaagtgct ttaa
//
LOCUS       DAOTKS010000095         4594 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_16263_length_4594_cov_3.545935, whole genome shotgun sequence.
ACCESSION   DAOTKS010000095 DAOTKS010000000
VERSION     DAOTKS010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16263_length_4594_cov_3.545935"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="P5236_02945"
     CDS             complement(<1..820)
                     /locus_tag="P5236_02945"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007479051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRU63352.1"
                     /translation="MSDVKICINPGHGGYDSDDRNVVIPPFTSGNPNGFWESQSNLDK
                     GLQLRTMLENAGATVIMTRTTNTTADDLPLSQIVAIANSANVDYMLSIHSNAGNGVAN
                     YVLQLYAGKDPNDTYTYPTPTPYSEESREVSTIVAQNLYSNEITNWSAPYSVRGDKTF
                     ARTAMGWSDGYGVLRGLAVPGAISEGSMHDYIPETYRLMNMDYKWLEAWHFFKSFCQY
                     FNGGEIPTGNIGGSVRDSRNVIQETYYKLKGTRDELLPLNGALITLLETNETYTT"
     gene            complement(903..1823)
                     /locus_tag="P5236_02950"
     CDS             complement(903..1823)
                     /locus_tag="P5236_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63353.1"
                     /translation="MKTKKIFSFLMFIFLGTYLVSAQTITITKRYKIDDNKIRGYYPI
                     LSTNGDKLLFTSDSYKGLSLYDFNTKTVIKITDEEGAGYEPIFDESESKIFYRKTSYQ
                     NNRKFEAIESFDLNTKQKKEMISPQRTIRQLQPYHKGLLVSVDRKLLKATFGKVQQEI
                     PVYVCSEDLKLVIYKNGNRQELNPIAGEDVSYIWVSLSPDKTKILFTATTKGTFVCDL
                     NGKIIASLGYLNAPVWYDNDFVVGMQDKDDGHFITESKIIMMPLKLDKKHILSLPNEI
                     AMYPSASAKAKKVAYNTLAGIIQIVELNIK"
     gene            complement(1867..4362)
                     /locus_tag="P5236_02955"
     CDS             complement(1867..4362)
                     /locus_tag="P5236_02955"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HRU63354.1"
                     /translation="MKKIIIGFILVLAIFLQTLQAQNTITLNNRIYRIDTLVQKHKIG
                     PGAYHTYYDLPTYPLKVHIVEIDLTNPHIQIETCLANDKALTIERPSSMARRKDYPGH
                     DVVAATNGGFYFYTDPVDIGIPRSAQFINEECMVAQPYGRASFILGKNKKPFVDRFSF
                     NGLIKTGTNSHKLNMINTVNQEVYTEENANIMILYTDKFGGKTPTDNTGIEILIKPKE
                     GTTFTYRSNQDYPAVVEKILPSGGNSVIPTGKAVLFGRGNSRDFLTNINEGDEITISL
                     EFELRTTPNLLKEIKESIGGSDHKILNNGQLGEGDYGAYYGSHPRTGMGISKDSSTVY
                     LIEVDGRQPGFSMGASLYEFAEIFKSAGAWNAVNLDGGGSSIMIVNKQIVSKFSESTE
                     RAVGNGVLIVSQAPVDNVISHIEFEPKKLEIPMYSELCPKIYGYNQYGLLINKDVKGF
                     QLSCSPELGQIINDSIFVASNIKQTGVLTATFNNLSVAKMVTIVDANINIRLDSVLID
                     HRTQYPIEVLSQNNKTIMNIPPAALSWNIRDPEICSINENGILKGLKNGSTMVIGTLG
                     DFKDSLKVIVEIPTSGRMIGDNFNVENWTLNASASLNAVLNTENLPSNWTHGAAVNYV
                     YTSTRAPFIKLLRNVPMYGLPDTLKLIINIGDINISKALLSLRANNATVSASKEFTNI
                     PKNRDTELSVAMSELFDATDIAIYPVWFDNINFYLGTQTTGQAYTLALKEIVLCYKDV
                     EISGLPSVLLSQYEVYPNPLDGTILNIRIKDSDSKLLQAQLFHLNGQSVVSQQLNPVS
                     GMATFKIGKLPAGTYLLKLYSNNETETIKVIIK"
BASE COUNT         1447 a          775 c          727 g         1645 t
ORIGIN      
        1 ctgttgtata agtttcattt gtttctaaaa gtgttattaa agctccattg aggggcagta
       61 attcatctct tgttcctttt aatttataat aagtttcttg aataacattt cggctgtctc
      121 gcacagaacc acctatgtta cctgtcggaa tttcaccgcc attaaaatac tgacagaaag
      181 atttaaaaaa atgccaggct tctaaccatt tataatccat attcataagc ctataggttt
      241 caggaatata atcatgcatt gatccttcag aaatagcccc tggaactgct aagccacgaa
      301 gaacgccata gccgtcgctc caacccatag ctgtgcgtgc aaatgtttta tctcctctca
      361 ctgaataagg agcagaccag tttgttattt cgttagaata taaattttga gccacaatgg
      421 tactaacttc tcgactttcc tccgaataag gagtaggagt gggataggtg taggtgtcat
      481 ttggatcttt tcctgcatat agttgcagca cataattagc gactccattt cctgcattac
      541 tgtgaatgga taacatataa tccacatttg ccgaattagc tattgctaca atttgggaaa
      601 gaggtaaatc gtcggctgta gtatttgtcg ttcttgtcat tattactgta gcaccagcat
      661 tttctaacat tgttcttaat tgcagacctt tatccagatt actttgtgat tcccaaaaac
      721 catttggatt accagaagta aaagggggta ttacaacatt cctgtcatca ctatcataac
      781 ctccgtgacc aggattgatg caaattttaa catcagacaa gttttgtgat aaaacattga
      841 aggaggtaaa aaatataagg gataaaaaaa ttatgttttt ctttttcata gcgataaatt
      901 tattatttta tatttaattc tactatttga ataattccag cgagagtatt ataagctact
      961 ttttttgctt tagcagaagc acttggatac atagcaattt cgtttggcaa actcaaaatg
     1021 tgttttttgt caagttttaa tggcatcatt attatttttg attcagtaat aaaatggccg
     1081 tcgtctttgt cttgcattcc tacaacaaaa tcattgtcat accaaaccgg agcatttaaa
     1141 tatcctaatg aggcaataat ttttccattc aaatcacata caaaagttcc tttggtagta
     1201 gccgtaaaaa gtattttagt tttatcaggc gacaaagaaa cccatatata acttacatct
     1261 tctcctgcaa ttggatttaa ttcttgacgg ttcccatttt tgtaaataac caatttcaaa
     1321 tcttctgaac atacataaac aggtatttct tgttgaactt ttccaaaggt agcttttaat
     1381 aattttctat ccacagaaac taataatcct ttgtgataag gttgtagttg tcttatagta
     1441 cgttgaggag aaatcatttc ctttttttgt ttcgtattaa gatcaaaact ttctatggct
     1501 tcaaacttac gattgttttg ataacttgtt ttcctataaa aaattttaga ctcggattca
     1561 tcaaatatgg gctcatagcc agcaccttct tcgtcggtaa ttttaattac tgttttagtg
     1621 ttaaaatcat ataatgataa gcctttataa ctgtcggagg taaataataa tttatctcca
     1681 ttagtgctta aaataggata atatccacga attttattat catcaatttt gtatcttttt
     1741 gtaattgtta tcgtttgggc agaaactaaa taagtgccta aaaagataaa cattaagaat
     1801 gaaaaaattt tttttgtttt catagtgaat tattttaatt taaattaata ataacaattt
     1861 tttattttat tttattatca cttttattgt ctcagtctca ttgtttgaat aaagtttcaa
     1921 caaataagtt ccagcgggta atttaccaat tttaaaagta gccattccac ttactggatt
     1981 cagttgttga gaaaccacag attgaccatt taaatgaaag agttgagctt gcagaagttt
     2041 tgagtcgcta tcttttattc ggatgttcaa aatagttcca tccaatggat tcggataaac
     2101 ttcgtattga gaaagcaata cagaaggcaa tcctgaaatt tctacatctt tgtagcaaag
     2161 aacgatttct ttgagagcca gcgtataagc ttgtcctgtt gtttgtgtgc ccagatagaa
     2221 attaatgtta tcgaaccaga caggataaat ggcaatatct gtggcatcga aaagttcact
     2281 catggctact gatagttctg tatctctatt tttaggtata ttggtaaatt cttttgaagc
     2341 agaaacggta gcattatttg ctctcaaaga caaaagagct tttgaaatat taatatctcc
     2401 aatattaata attagtttta aagtatctgg taaaccatac atgggaacat ttctgagaag
     2461 cttaataaaa ggtgctcttg ttgaagtata tacataatta accgcagctc catgtgtcca
     2521 gttgctgggt aaattttctg tgttaagcac tgcatttaag gaagcggaag cattcaatgt
     2581 ccagttttca acattgaaat tatcgccaat cattcttcct gaagttggaa tttcaacaat
     2641 tactttgaga gaatctttaa aatcgcccaa agtaccgatg accatagtac tgccattttt
     2701 caaacctttt aagataccgt tttcgttaat ggaacaaatt tcaggatcac gaatgttcca
     2761 gctcaaggca gcaggtggaa tattcataat ggttttatta ttttggctta acacctcaat
     2821 agggtattga gtgcggtgat caataagtac tgaatctaat cttatattaa tgttagcatc
     2881 aacaatagtg accattttag caacacttag attattaaaa gtagctgtaa gtacccctgt
     2941 ttgttttatg ttacttgcta caaatataga atcgttgata atttgaccta attcaggtga
     3001 acaacttaac tgaaaacctt tgacgtcctt attaatgagt aaaccatatt gattataacc
     3061 ataaattttg ggacataatt cactatacat aggtatctcc aatttttttg gctcaaattc
     3121 tatatgtgag attacattgt caactggagc ttgagatact attaatactc catttcctac
     3181 agcacgttca gtactttcag aaaatttgct aacgatttgt ttgtttacaa tcataatgga
     3241 tgaacctccg ccatcaaggt tgacagcatt ccatgcacct gcagatttaa aaatttctgc
     3301 aaattcatat aaagaagctc ccatcgaaaa tcctggttga cgtccgtcaa cttctattaa
     3361 ataaaccgtg gaactatctt ttgaaattcc cattcccgta cgaggatgag acccataata
     3421 ggcaccataa tcaccttctc ctaattgtcc attatttaaa attttatgat cacttcctcc
     3481 tattgattct ttaatttctt tcagtagatt cggtgtggtt cttaattcaa attccaaaga
     3541 aatagtgatt tcatctcctt cgtttatatt ggtaagaaaa tcgcgtgaat ttccgcgtcc
     3601 aaacagaaca gctttcccgg taggaattac tgaatttcct cccgatggta aaattttttc
     3661 aacaactgct ggataatctt ggttggaccg ataagtaaaa gtagttcctt cttttggctt
     3721 gattaaaatt tcaataccag tattatctgt tggcgttttt cctccgaatt tgtctgtata
     3781 taaaatcatt atattggcat tttcttcagt ataaacttct tgattaaccg tattgatcat
     3841 attaagttta tgtgaattgg tacctgtttt aattaaacca ttgaaggaaa aacgatctac
     3901 aaatggtttt ttgtttttac ctaatataaa agaagcacgt ccataaggtt gagctaccat
     3961 gcattcttca ttgataaatt gggcgcttcg tggaattcct atatcaacag ggtcggtata
     4021 aaagtaaaac cccccatttg ttgctgccac tacatcatga cccggataat cttttcttct
     4081 tgccattgaa cttgggcgct cgatagtcaa agctttatca tttgccaaac aagtttctat
     4141 ttgaatatgg ggatttgtta gatctatttc aacaatgtga actttgaggg gataagtggg
     4201 taaatcataa taagtatgat aagcacctgg acctatttta tgtttttgaa ccaacgtatc
     4261 aatacgataa atcctgttgt ttaaggtaat tgtattttgt gcctgtaatg tctgtaaaaa
     4321 aatggctaaa actaaaataa aacctataat aatttttttc ataggtagtg aatattttta
     4381 ataattaaac taaagttgaa taaggggtta ttcaacttta gttaatgaaa ttaatatcaa
     4441 ttttctttat aaaattgtca ataaattttc tattcatttt tatttatttt atcactattt
     4501 tgacactttc atatttttca ttgtttgaat agagtttcaa caaataagtt ccagcaggta
     4561 atttaccaat tttaaaagga gctattccac ttac
//
LOCUS       DAOTKS010000096         4559 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_16417_length_4559_cov_3.523091, whole genome shotgun sequence.
ACCESSION   DAOTKS010000096 DAOTKS010000000
VERSION     DAOTKS010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16417_length_4559_cov_3.523091"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3026)
                     /gene="sprA"
                     /locus_tag="P5236_02960"
     CDS             complement(<1..3026)
                     /gene="sprA"
                     /locus_tag="P5236_02960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HRU63355.1"
                     /translation="MRRVKKSVRVFVFFNSFLIANFSFVSLFSQVFSNQAVESQQSSS
                     DSILFSIKKLSQDTYEDITTQLPLDAPTPSNVKSVIEYDPQTNTYILRTYVGEIEIAT
                     PFLMTEKEYYDYTLKKEMENYWREKNVGSQIDNEDKFRLTDMKFDIGPAEKVFGPGGV
                     QVRTQGSAEILFGYKTNKIDNPALTERMRKTGIFDFEEKIQMNVNGKVGDKVNFNLNY
                     NTESSFEFDQKMLKLNYKGKEDDIIKSIEAGNVSMPLNSSLIPGSSALFGIKTDLQFG
                     KLSVSAIASQQESETKTISTRGGAQTTKYEINIDNYDENRHFFLSHYFRNNYENAMSK
                     LPYVMSGVTITRMEVWITNKRGNYEQARNIIAFEDLGETDRIDNPHWQPMSTNLLPYN
                     KANTLYDEIVSLPNVRDIQQTNNILSSQYGALGIVGGQDYEKIESARKLDPSEYTFNP
                     TLGYISLRNMLQPDEVLAVAFEYTYGGQTYQVGEFSTDPITAPNALIVKLLKSTTQSP
                     SLPSWDLMMKNVYSLGATQIQPENFILEIVYRNDSVGTELRYITEGNIKNKLLLQVMN
                     LDRLNSKNAPQPDGKFDYVEGYTIFSSSGKIFFPVLEPFGSHLKQAIGNDQIAEKYIF
                     QELYDSTLVMAQEFSEKNKFRIIGEYKASSRNEIRLNAMNIPRGSVVVTAGGKTLVEN
                     VDYTVDYIMGTVNIINTSLLESGTNIDVKLENRSMFNMQRKTLLGTHLEYQFNRDFSL
                     GGTIMHLSEMPLTTKVNTGNEPISNTIWGLNTSWRKESQWLTNQLDKLPFVNATKPST
                     IALNAEVAQLIPGHSSVISAAGLAYIDDFESTQTTIDIHYPAFWYLASTPYNPNGGLF
                     PEAALSNNVEYGKNRALLAWYYVDPILNANSRNTPPNLRNNVESQSNHLTRNVLEEEI
                     FPNKERLATQTSRLTILNLSYYPTERGPYNLDADNINPDGTLMNPQQRWGGIMRKLDV
                     TDFETANIEYIEFWMMDPFIMDKDGTQRGGDLYFNLG"
     gene            complement(3105..3695)
                     /gene="ruvA"
                     /locus_tag="P5236_02965"
     CDS             complement(3105..3695)
                     /gene="ruvA"
                     /locus_tag="P5236_02965"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRU63356.1"
                     /translation="MIDYIKGEIVELTPTYVVIETGGIGYFINIALPVFSLLSGKNSV
                     KLYIYEVIREDAYLLYGFSTKDERQLFLLLISVSGIGANTARMIMSSYSVEELQGIIA
                     SENDSALSSIKGIGTKTAQRIIVDLKDKILKITSNKENIPAFKDTTSQIKEEAIAALI
                     MLGFSAAVSQKAIDSILKKQSVANVEDLIKQVLKII"
     gene            4107..4481
                     /locus_tag="P5236_02970"
     CDS             4107..4481
                     /locus_tag="P5236_02970"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HRU63357.1"
                     /translation="MKVLYYEKKLDKESIDARVLYRYQRFEEIIKEAELLCHKNFYNA
                     SVNILYYACYYAISALFLKDGIEAYSHKGMKTLFGLYYVKTEKIPIEQATTYFTLFEK
                     RHSSDYDDFILILILIARWWKN"
BASE COUNT         1495 a          753 c          724 g         1587 t
ORIGIN      
        1 cctaaattaa aatacaaatc accacctctc tgtgttccat ctttatccat aatgaaagga
       61 tccatcatcc agaattcaat gtattcaatg ttagctgttt caaaatcagt cacatcgagt
      121 tttcgcataa taccacccca gcgttgttgt ggattcatta aggttccatc aggatttata
      181 ttatctgcat ccagattata aggacctctt tcagtaggat aataagataa atttaaaata
      241 gtcaaacgtg aagtttgtgt tgccagtcgt tctttatttg gaaaaatttc ttcttccaga
      301 acattacgtg tcaaatgatt agattgagat tcaacattat ttcttaaatt tggaggtgta
      361 tttctgctgt ttgcatttaa aataggatca acataatacc aagctaacaa agctctattt
      421 ttaccatatt caacattgtt acttaaagct gcttctggga aaagcccacc atttggatta
      481 taaggtgtgc ttgccagata ccagaaagct ggataatgaa tgtctatggt tgtttgtgtt
      541 gattcaaaat cgtcaatata agccagacca gccgcactga taacagaaga atgtcctgga
      601 atcaattggg caacttctgc atttaaggca atagttgaag gtttggtagc atttacaaat
      661 ggcaacttgt ctaattggtt ggttagccat tgtgattctt ttctccacga agtattcaat
      721 ccccaaatgg tgttggaaat gggttcattg ccagtattta ctttagttgt caaaggcatt
      781 tcggaaaggt gcataatggt tccccctaaa ctgaaatcac gattaaattg atattccaga
      841 tgagtaccca acagtgtttt tcgctgcata ttaaacatgg aacgattctc gagttttaca
      901 tcaatattgg ttcctgattc cagaagagaa gtattgataa tattaacagt ccccataata
      961 taatcaacag tgtaatcaac attttccacc aatgtttttc ctccagcggt tactactacc
     1021 gatcctcgtg gaatattcat cgcatttaac ctgatttcat ttcttgaact tgctttgtat
     1081 tctccaatga ttctaaattt atttttttca ctgaattcct gagccattac aagggtagaa
     1141 tcatataatt cttgaaaaat atatttctct gcaatctgat cattgccaat tgcttgtttt
     1201 aaatgagagc caaatggttc cagcacaggg aaaaagattt ttccactgga agaaaaaata
     1261 gtatatcctt ctacataatc aaacttacca tcaggttgag gtgcattttt ggagttcagc
     1321 ctatcaagat tcattacctg tagtaataat ttatttttaa tatttccttc ggtaatatat
     1381 cgaagttctg taccaaccga atcgtttcga taaactattt ccaatataaa attttcaggt
     1441 tgaatctgag ttgctcctaa actgtaaacg tttttcatca ttaaatccca cgaaggcaaa
     1501 gaaggagatt gagtagtgct ttttaataat ttgacaatca atgcatttgg tgcagttata
     1561 ggatcggttg aaaattcacc tacttgataa gtttgcccgc cataagtata ttcaaaagcc
     1621 actgccagca cttcatcagg ttgtaacata ttgcgtaaag aaatataacc taaggtagga
     1681 ttaaaagtat attcagaggg atccagttta cgagcacttt caattttttc ataatcttgt
     1741 cctcctacta ttcccaacgc tccatattgt gaagaaagaa tattgttagt ttgctgaata
     1801 tcacgaacat ttggcaaaga aacaatttca tcatataatg tatttgcctt gttataaggt
     1861 aaaagattgg ttgacattgg ttgccagtga ggattatcaa ttcgatcggt ttctcccagg
     1921 tcttcaaaag caataatatt tcttgcttgt tcataatttc ctcgtttatt ggtgatccaa
     1981 acttccattc ttgtaatagt aacgccagac atcacatacg gaagtttact cattgcattt
     2041 tcataattat ttctgaaata atgacttaaa aagaaatgcc gattttcatc ataattatca
     2101 atattaattt catatttagt agtctgagca cctcctcgtg tacttatggt tttggtttct
     2161 gattcttgtt gagatgcaat ggcactgaca cttaattttc cgaactgcaa atcggttttt
     2221 attccaaaca aagctgagct tcctggtata agagaactat tcaagggcat acttacattg
     2281 cctgcttcaa tgcttttaat aatatcatct tcttttcctt tataattgag tttaagcatt
     2341 ttctggtcaa attcaaatga cgattcagta ttataattca aattaaaatt gactttgtcg
     2401 cctactttac catttacatt catctgaatt ttttcttcaa aatcgaatat gcctgttttt
     2461 ctcattcttt cagtcaaagc tggattatct attttattgg ttttgtaacc aaataaaatt
     2521 tcagcagagc cttgtgttct aacttgtaca ccgccgggac caaaaacttt ttcagcaggt
     2581 ccaatgtcaa atttcatgtc ggtcaaacga aatttatctt cattatctat ctgactacca
     2641 acattttttt cacgccaata attttccatt tcttttttca aagtgtagtc ataatattcc
     2701 ttttcagtca ttagaaaagg agtagcaatt tcaatttcgc cgacataagt acgtaaaatg
     2761 tacgtattcg tttgaggatc gtattcaatt actgatttaa cattagaagg agtgggtgca
     2821 tcaaggggta attgagttgt aatatcttcg tatgtatcct gacttaattt cttaatagag
     2881 aaaagaatag aatctgaact tgactgttga gactcaacag cttgattaga aaaaacttga
     2941 ctaaataaac tgacaaagct aaagttagct atcaaaaagc tattgaaaaa tacaaaaaca
     3001 cgtaccgatt ttttcaccct tcgcataaat tgtttttcac ttttgtagaa agctattaga
     3061 ttaaatcaag tagaaattac ccatttatca atgtgcttta aaagtcagat tattttcaaa
     3121 acctgtttaa ttaaatcttc tacgttggca acagattgtt ttttcaatat agaatcaatt
     3181 gctttttgag aaaccgccgc agaaaaacca agcataataa gggcagcgat agcttcttct
     3241 tttatttggg aggttgtgtc cttaaatgcg ggaatatttt ctttattgga agtaattttt
     3301 aaaatcttat cttttaagtc cacaatgatt cgttgagctg ttttggtacc aattcctttt
     3361 atagagctaa gagcagaatc attctcactg gctattatcc cttgaagctc ttcaacggaa
     3421 taagacgaca taatcattcg tgcggtattt gctccgattc ctgaaacaga gatcaataac
     3481 aaaaaaagct gcctttcatc ttttgtgcta aagccataaa gtagataagc atcttcacga
     3541 atcacctcat aaatatataa cttaactgag ttttttccac ttaataaaga aaacacaggg
     3601 agagcaatat tgataaaata gcctattcct cctgtttcaa tcaccacata agtaggtgtt
     3661 aattctacaa tttcaccttt tatataatca atcatctggt taaaatttgg tgtaaaagta
     3721 agaatttatc tggaaatata ccaactttat caagctatag aataaaaaac cttatttttt
     3781 agttaacgtt gatttctata aaaaattacg attaagcaat caaaaagaaa agaaagaatt
     3841 tttgctaaag gaaataaaaa ggcacaattt ctctactcct aaatagcttt tgaaaataaa
     3901 aaatagttta aaaatagttg taaattagta tttttagttt acggtaggag aggggagagg
     3961 gagagagaag gatgaggtgc aaatatttga ataacgttga ccattttttt tattaaaaaa
     4021 tcctatcttt gtaaaatttt tagaatatat aagatgatgc cagatagaaa ggccattcaa
     4081 aactctcttt caaatcaaca ttttgaatga aggtattgta ttatgaaaaa aagttggata
     4141 aagagagtat agatgctcgg gttctttatc gttatcagcg atttgaagaa attattaaag
     4201 aagctgaact actttgtcat aaaaatttct ataatgcatc agtaaatata ctttattatg
     4261 catgttacta tgcaatttca gctctttttt taaaagatgg aattgaagca tatagtcata
     4321 aaggaatgaa aactctcttt ggtttgtact atgttaaaac tgaaaaaatt cccattgagc
     4381 aagcaacaac atattttact ctttttgaaa aaagacatag tagtgactat gatgacttta
     4441 ttttgatttt gattttgatc gcgagatggt ggaagaatta atttctcaaa caaaggattt
     4501 tatcgctttt attaaaaatt tattaggctt ataatatggg aaaagattat tttgtacat
//
LOCUS       DAOTKS010000097         4482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_16731_length_4482_cov_3.144793, whole genome shotgun sequence.
ACCESSION   DAOTKS010000097 DAOTKS010000000
VERSION     DAOTKS010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16731_length_4482_cov_3.144793"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="P5236_02975"
     CDS             complement(<1..313)
                     /locus_tag="P5236_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRU63358.1"
                     /translation="MKRTLTFILILYLSFVAFIVSAQSNIRMNNYWIELNFINPASIY
                     DKYEAVFSIAARDQWAGINGAPQTFFASASTYLEKQHIQLGLSLIQDKAGYTSLTDVK
                     LT"
     gene            complement(326..3913)
                     /locus_tag="P5236_02980"
     CDS             complement(326..3913)
                     /locus_tag="P5236_02980"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRU63359.1"
                     /translation="MHMYNYRLFKFSWVVVNAFLAVINCYSQINVNESFKTGVAGTNI
                     KLGGSTYLTSGVVDPENDGWLRLTNSVRNEKGYAYIDANLPSTLGVLIDFEYKTWRSG
                     TDVNFVGGDGFSVFLFDATSTFTIGGYGGSLGYAPHTGEGAIRGLGGGYIGIGFDEYG
                     NFSNPTEGRIGGPGQRCNSITLRGPTTNDPRTTNKYLASIQLQPDNRSDINSIDFNTL
                     QDKRPSDSQFYRRVKIAITPTGSSSNPKYTITVRWRTSPNGEDVTLLTYTTETAPPPN
                     LKLGFAASTGGDVNYHEIRNLSVQTPGGVSTFKECDKGTLNVGEDVTYTIHVRNSTPA
                     PINNLILTDTLKDEKGNILNLASQNFTLKSITFDNNEDDSNTASGYTSGIPIISGFTN
                     PFTSMLSLNANSSSFFTIVGSIKNDPALEGASFINSVKIDPSLTGITDQDFSDNYSFA
                     VASVNITTPDLKISMFVDKPCADPVNGNIISILVSNISSVPINASDPKIQIIVTDTIP
                     PGYTFIKPPDGTWAVRQNGNVLRFNRNGTLNPGATFEAIQIKVFPPATGNILPNYATV
                     GFENNAIVEKHLENNRASVSITAIPSPPIVVSPVKYNLGEEPKPLSAEGTNLIWYTDL
                     NSPGTTVAPVPVTTSLGSTTYYVSQKNGECESQLVPIVVIVETGNIVIVDTTVCESYL
                     APDDSVYTTSGIKIIHFKTEEGQDTTILVNLTVHYNSTKFLSVTACDSYLAPDGEIYT
                     DSGRKTVIIPNAIGCDSIITIDLTIGKSTSDTINVKACDTYISPSGRIFHESGTIKDT
                     IPNKAGCDSIITIHLMVKKTTTVEVSPTVCDTYISPSGKVYHNSGTIKDTIPNKAGCD
                     SIITIHLEVNKTTRTEISPKVCDTYISPSGKIFHESALIIDTIPNKAGCDSIITIHLE
                     VANSSKANLDTTVCDTYTSPSGKIYYDSGLIKDTIPNKAGCDSILTIHLQVNKSSATT
                     LFVSTCDAYMAPDGQIYTDSGVKEAIIPNKAGCDSTIVIYLEIGKNTEKTIDITACDS
                     YVAPDGVIYTDSGTKTAIIPNVFGCDSIITIHLIINQSSSVSQVIYMFEGDKYSINKH
                     KYYKEGVYNDTLVAKNGCDSVITTEIKLIMIPNTITPNGDGKNDFFLPGYHVKIYNRN
                     GILLFEGNNGWDGTHKGQPVGPDTYYFVLYFNSASDGVKTKEGYITVIR"
BASE COUNT         1435 a          790 c          747 g         1510 t
ORIGIN      
        1 aagtaagttt gacatcagta agagaagtat atccagcttt atcctgaatt agtgacaatc
       61 caagttggat atgttgtttt tcgagataag tgctggctga agcaaaaaaa gtttgtggtg
      121 ctccattaat gcctgcccat tgatcacgtg cagcaatact aaaaactgct tcatatttat
      181 cataaataga ggctggattt ataaaattca attcaatcca gtaattattc attcggatat
      241 tcgactgtgc cgaaacaata aaagcgacaa atgataaata taatattaat atgaaagtta
      301 atgttctttt catcgtatta tttacttagc gaataacagt tatataacct tctttcgttt
      361 taacaccatc tgatgccgaa ttgaaataaa gtacgaaata ataggtatca ggacctaccg
      421 gttgtccttt atgcgtacca tcccaaccat tattcccttc aaacaacaga atcccattcc
      481 ggttataaat tttaacatga taaccaggaa ggaaaaagtc atttttccca tctccattcg
      541 gcgttatggt gttcggtatc ataattaact ttatttcagt tgttatcaca ctgtcgcaac
      601 cattttttgc cacaagagta tcattataaa ctccttcctt gtagtattta tgtttgttaa
      661 ttgaatattt gtccccttca aacatataaa ttacttgact aacactactg ctttgattaa
      721 tgattagatg aatagttatt atgctatcgc aaccaaaaac attaggtatt atagcagttt
      781 tagtgccaga atcagtataa atgactccat caggagccac atacgaatca caggcagtaa
      841 tgtcaatagt cttttcagta ttcttgccta tctccaaata gatcactatc gtactgtcac
      901 atcctgcttt gttggggatg atagcttctt taacccctga atcagtataa atttgtccat
      961 caggtgccat ataagcatca caggtactta caaaaagggt agtagcactg ctcttgttta
     1021 cctgtaaatg gatggtaaga atgctgtcac atcctgcttt attcggtata gtatccttta
     1081 ttaatcctga gtcataatat atttttccac tgggagaagt gtaagtatca cagacggtgg
     1141 tatcaaggtt agctttacta ctgttagcca cctctagatg aatggttatg atactatcac
     1201 aacctgcttt gtttggtatg gtatctatta tcagagccga ctcatgaaat atttttccac
     1261 tgggtgaaat gtaagtgtca catactttcg gcgatatttc tgttctggtg gttttgttta
     1321 cctccaggtg gatagttatg atgctgtcac aacctgcttt gtttggtatg gtatctttta
     1381 ttgttcctga attatgataa actttaccgc tcggagaaat gtacgtatca cataccgttg
     1441 gacttacctc gacagttgtt gtcttcttta ccatgagatg gatggttatg atgctgtcgc
     1501 agcctgcttt gttcggtatg gtatccttta tcgttcctga ctcatgaaat attcttccac
     1561 tgggtgaaat gtaagtatca caagctttta cgttgatggt atcagatgta ctctttccaa
     1621 tggtcaaatc tattgttata atactgtcac aaccaattgc attcggaata attacagttt
     1681 ttctaccaga atcagtatat atttccccat ctggagcaag atacgaatca caagcagtta
     1741 cgcttaaaaa tttagtagaa ttgtaatgaa ccgtcaagtt aacaagaatt gttgtatctt
     1801 gaccctcttc tgttttaaaa tgaattattt ttattccaga agttgtatac acagaatcat
     1861 caggagccag ataagattca caaacagtag tatcaactat cactatgttt cccgtttcta
     1921 ctattaccac aatggggacc aactggcttt cacattcacc atttttttgg cttacataat
     1981 aagtagtgct tccaagagaa gttgtgacag gcacaggagc aactgtagtc cctggactat
     2041 tcaaatcggt gtaccatatc aaattggttc cctcagctga taaaggtttt ggctcttctc
     2101 ctaaattgta ttttacaggg gaaaccacaa taggaggaga aggaattgca gttattgaaa
     2161 cactcgctct attattttcc agatgttttt ctacaatagc attattttca aagcctactg
     2221 ttgcataatt aggtaaaata ttccctgtag ctggaggaaa cacttttatc tgtatagctt
     2281 caaaggttgc gccgggattt aaagtaccat ttctgttgaa tctcaaaaca tttccatttt
     2341 ggcgcactgc ccaagtacca tcaggaggtt taataaaagt atagccaggg ggaatggtat
     2401 ctgttacaat aatttgaatc ttaggatcgg aggcattaat aggaacagat gatatattag
     2461 agaccaggat agaaataata ttcccattaa cagggtcagc acaaggttta tcaacaaaca
     2521 ttgaaatttt taaatctggg gttgttatat taacacttgc gacggcaaag gaataattat
     2581 ctgaaaaatc ttgatctgtt attccggtca gtgagggatc aatttttact gaatttataa
     2641 aagatgctcc ttctagtgca gggtcgtttt ttatgcttcc cacaatggta aaaaaggatg
     2701 aactatttgc attcaaactt aacatgcttg tgaaaggatt agtaaatcca cttataattg
     2761 gaataccact cgtatagcca cttgcagtgt tggaatcatc ttcgttatta tcaaatgtaa
     2821 tactttttaa tgtgaaattt tgtgaggcta aattaagaat atttcccttt tcatctttaa
     2881 gtgtatctgt taaaattaaa ttattaatcg gagcaggcgt agagtttcta acatggattg
     2941 tataagtaac atcctctcca acattaagag tacctttgtc acattcttta aaagtactta
     3001 cacctcctgg ggtttgaaca gataaatttc ttatttcgtg ataattaaca tcaccgcccg
     3061 ttgaagcggc aaaacccagt tttaaattag gaggaggagc agtttcagtt gtataggtta
     3121 ataaagtaac atcctctcca ttaggagaag ttcgccagcg gacagtgatg gtatatttcg
     3181 gattactgga acttcctgta ggtgttatgg caattttcac ccttctgtaa aactggctgt
     3241 cggaaggtct tttatcctgt aaagtattaa aatctatgga attaatatct gatctattat
     3301 caggctgtaa ctgaatagac gctaaatatt tattagtagt acgtggatca tttgtagtag
     3361 gtcccctcaa agttattgaa ttacatcttt gcccaggacc gcctattcta ccttctgttg
     3421 ggtttgaaaa attaccatac tcatcaaaac cgattccaat atatccacct ccaagccctc
     3481 taatagcccc ttcacctgta tgtggcgcat aacccagaga acctccataa ccaccaatag
     3541 taaaggttga agtagcatca aaaagaaaaa cagaaaatcc atcccctcca acaaaattga
     3601 catctgtacc tgagcgccag gttttatact caaagtctat tagaactcca agagtagaag
     3661 gaagatttgc atctatataa gcatatccct tttcatttct cactgaattt gtcaatcgaa
     3721 gccaaccatc gttttcagga tcaactactc ctgatgttaa ataagtcgag ccacccaact
     3781 taatatttgt accggcaaca cctgttttaa aactctcatt aacattaatc tggctgtagc
     3841 aatttataac agctaaaaat gcatttacga cgacccaaga aaatttaaat aaacgatagt
     3901 tatacatatg catatttaga acggattccc cctttcaaaa ggggtatatt ttatatataa
     3961 aaaattgatt attatttgtt taatatattt tttaaagtaa tttcttgttt acagtgtaat
     4021 tattgattgt ttatcacata ttaaatatta aacattattt tattttttat aaatatacaa
     4081 aaaaattcaa taaaataact ttgttagaaa aaaaaatagt atttttcttg cgtacattaa
     4141 acgtacatta aatttactgg aatattctta aaagcctatt gtattggctt ttagatcatt
     4201 ttttacaaaa ttttggaaaa aaatccgata taaaatttaa aaattgatta cacaaatttt
     4261 tttatggtga gaaaatgtgt ataattcaag aagaacatta taaaaaaact ttcgtcgaag
     4321 cagataatga ttttttgaaa ttaattaatt aaccaaataa gataataagg ttataatcaa
     4381 gaagatattt caaagttatt agggttcaat gtttttatat aaggttttaa taaagaaatt
     4441 ccttaaaatt gactatcctc gccgcaagca gcgaggtatt ac
//
LOCUS       DAOTKS010000098         4478 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_16749_length_4478_cov_3.875650, whole genome shotgun sequence.
ACCESSION   DAOTKS010000098 DAOTKS010000000
VERSION     DAOTKS010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4478
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16749_length_4478_cov_3.875650"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="P5236_02985"
     CDS             <1..820
                     /locus_tag="P5236_02985"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRU63360.1"
                     /translation="YVSENEKQEFLSTFGYLLNGLISTNLIQQKDESDKWINNQLVQW
                     YYQSGTQQVTMLITKKWNDTYSKWENYQKSEYEYLQQQPLSEIHQHWELAFWRNDFRY
                     DYFYDKNGNLEKKVLSLPIYEKWRPVINIFYDNYIQEMSNVIKAQYDFWGGEINEPVT
                     SYIPFLFNDEWTIKKAQYIEIDYDDFIDRTNTITNENNNIFIYPNPSEGIYYLNHSSA
                     EIQSWTVTALNGQMIISQKQPSSSVIDLTDCPQGIYILKMQTSDGQFIQKIIKK"
     gene            898..2970
                     /locus_tag="P5236_02990"
     CDS             898..2970
                     /locus_tag="P5236_02990"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HRU63361.1"
                     /translation="MVTTSIASTALAGNPFFETYKTPHQTIPFNLIKNEHYIPAFEEA
                     IRQHNKEIETITNNSKSPTFKNTIEALEYSGRLLDKVSSPFFNLLSAETNDELQQIAE
                     KISPELTEHFNSIYLNDKLFQRIKIVYGQKDKLPLTPEQNMLLQQTYDAFVKNGANLS
                     DTDKEIFRQYTKGLNLLTLQFGQNVLKETNQYQLLIKDKNVLSGLPEDLLNTLAENAR
                     KAGKDGWLIDLKQTTYTPMMKYADQRDLRRELYLAYNTRCMKGGEYDNQEIIKKIVNL
                     RLKIAQLLGYSTYADYVLVHRMAENKKNVYDLLNKLLTAYKPTARKEYEEIQTYADAI
                     GAYFEVQPWDWSYYSEKLRSEKYQINDELLKPYFELEKVKNGVFGLATRLYGIHFTKN
                     PKIPVYHPEVEAYEVTDKDGKFIAVLYTDFHPREGKQSGAWMSEFKGQWKKNGKDSRP
                     QIVIVMNFTKPTATKPALLTFDEVTTLLHEFGHSLHGMLSDCTYPSLSGTNVYRDFVE
                     LPSQIMENWAFQKEFLDDFAAHYETNEKIPDELINKIKEAKNFHVAYACLRQLGFGYL
                     DMGWHTLSTDFEGNVVQFEKNSMIDTQILPDVPNTAISPTFTHIFSGGYAAGYYSYKW
                     AEVLEADAFSVFEKNGIFDQGTANSFLENILKKGGTEHPMILYKRFRGQEPTIDALLQ
                     RNGIVTNN"
     gene            3089..4478
                     /gene="pulA"
                     /locus_tag="P5236_02995"
                     /pseudo
     CDS             3089..4478
                     /gene="pulA"
                     /locus_tag="P5236_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444370.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
BASE COUNT         1560 a          700 c          783 g         1435 t
ORIGIN      
        1 ctatgtttct gaaaatgaga aacaggaatt cctttccact tttggatatt tgttaaacgg
       61 cttgatctca actaatctta ttcaacaaaa agacgagtca gacaaatgga taaataatca
      121 acttgttcaa tggtattatc aatctggtac acaacaagtt acaatgctta tcacaaaaaa
      181 atggaatgat acttattcaa aatgggaaaa ttatcaaaaa agtgaatatg aatatttaca
      241 acaacaacct ctatctgaaa ttcatcagca ttgggaattg gcattctggc gaaatgattt
      301 ccgttacgat tacttttatg ataagaatgg aaatttagaa aagaaagttc tttccttgcc
      361 aatttatgaa aaatggcgcc ctgttatcaa tatcttctat gataattata tccaagaaat
      421 gtccaatgta ataaaagccc aatacgattt ctggggtgga gaaataaatg aacccgttac
      481 ttcttatatt ccttttttgt tcaatgatga atggacaata aaaaaagctc agtatataga
      541 aattgattat gatgatttta ttgatagaac aaatacgatt accaatgaaa ataataatat
      601 ctttatttac cctaatcctt cggaaggaat atattatcta aatcattcga gtgctgaaat
      661 tcaatcttgg acagttactg ctttaaatgg gcaaatgatt atttctcaaa aacaaccttc
      721 ttcttctgta attgatttga cagactgtcc tcaaggaatt tatattctta aaatgcaaac
      781 cagtgatgga caattcattc aaaaaattat caaaaaatag aattttgttt tcaaaaaatg
      841 attcaaaaga taaatcaaaa atttaataaa aatcattcaa tcgtaaaatt ttttattatg
      901 gtcacaactt ccatagcttc tactgctctg gcaggcaatc ctttttttga aacttacaaa
      961 acaccgcatc aaactattcc ttttaactta attaaaaacg aacattatat tcctgctttt
     1021 gaagaagcta ttcgccagca taataaagag atagaaacta ttaccaacaa ttcaaagtcg
     1081 cctactttta aaaacactat tgaagcgtta gaatattctg ggagattact ggacaaagtc
     1141 agttcacctt tttttaattt attaagtgcc gaaactaacg atgaacttca acaaatagcc
     1201 gaaaaaattt caccagaatt aactgaacat tttaattcta tttatttaaa tgataaattg
     1261 ttccagcgga ttaaaattgt ttatggacaa aaagataagc ttccgcttac tcctgaacaa
     1321 aatatgctat tacagcaaac ctatgatgct tttgtaaaaa atggagcaaa cctttcggat
     1381 acagataaag aaatttttcg ccaatacaca aaaggattaa acttactgac ccttcaattt
     1441 ggtcaaaatg tactgaaaga aaccaatcaa tatcaattgc ttatcaagga taaaaatgta
     1501 ttaagcggtc tacccgaaga tttattaaac acactggctg aaaacgccag aaaagctggc
     1561 aaagatggtt ggttgattga tttaaagcaa actacttata ctcctatgat gaaatatgcc
     1621 gatcagcgag atctaaggcg tgaattatat ttggcttata atacccgatg catgaaaggt
     1681 ggcgaatatg ataatcaaga aataattaaa aaaattgtca atcttcgttt gaaaattgct
     1741 caactgcttg gatattctac ttatgcggat tacgtacttg ttcataggat ggctgaaaac
     1801 aagaaaaatg tatatgatct cttaaacaaa ttgttaacag cttataagcc gaccgccaga
     1861 aaagaatatg aagaaattca aacttatgct gatgcaatcg gagcttattt tgaagttcaa
     1921 ccgtgggatt ggagctatta ttcagaaaaa ttgagatcag aaaaatatca aattaatgat
     1981 gaattgttaa agccttattt tgaattggaa aaagtgaaaa atggagtttt tggtttggca
     2041 acgcggcttt atgggattca ttttacaaaa aatccaaaaa ttcctgttta tcatccagaa
     2101 gtagaagctt atgaggtaac tgataaagat ggaaaattta tagcagttct ttacactgat
     2161 tttcatccgc gtgaggggaa acaaagtggc gcttggatga gtgaattcaa aggacaatgg
     2221 aaaaaaaatg gaaaagatag tcgtccacag attgtaattg ttatgaattt tactaaacct
     2281 actgctacca aacctgcttt attaactttt gacgaggtaa ctactttgct acatgagttc
     2341 ggtcattcat tgcatggaat gttgagcgat tgtacttatc cttctctttc aggtactaat
     2401 gtttatcggg attttgtaga attgccttcg caaattatgg aaaattgggc ttttcaaaaa
     2461 gagtttcttg atgactttgc tgctcattat gaaacaaacg aaaaaattcc tgatgaactg
     2521 atcaataaga taaaagaagc caagaatttt cacgttgctt atgcctgtct tcgccaattg
     2581 ggatttggtt accttgatat gggctggcat actttatcta cagattttga aggaaatgtg
     2641 gttcaatttg aaaaaaactc tatgatagat acgcaaattc ttcctgatgt accgaatact
     2701 gccattagtc caacttttac tcatattttt tctggtggtt atgcagcagg ctattatagt
     2761 tataaatggg cagaagtgct ggaggctgat gctttctctg tgtttgaaaa aaatggcatt
     2821 tttgatcaag gtactgcaaa ttcttttttg gaaaatattt taaaaaaagg tggaacagag
     2881 catcctatga ttttatataa acgttttaga ggacaagaac caacaattga cgcgttgtta
     2941 caacgaaatg gcattgtgac gaataattaa agaattcaag aaaattgttt ttatgttttg
     3001 aaaaagaatt tttacttgtt aaatttttta aagattgttc gtaaatttat tataaatcaa
     3061 tcatttaaaa atgaaaaaaa tcatatttat gttttttctt ccaggctttt tatgttcctg
     3121 tcacaaggtt gcccattatt cttcttatga tgaatatcct gtttacaatg gttctgattt
     3181 agaattaact tacactccac aagcttcatt tttcagggta tgggcaccga ctgcttcaca
     3241 tgtaaaatta ttactttatg atgatggtga aaatggaagt gcttataaga tttatgatat
     3301 gaaacatgct gaaaatggca catggaaatt aaagataaaa aagatttgaa agggaaattt
     3361 tatacttttc aagtaaacat taatgacaaa tggttggacg aaacgccagg catgtgggta
     3421 aaagctacag gcgtaaatgg taaacgtgcc gctattattg atttttctga aactaatcca
     3481 gcaggctggc taaatgacca gcgtccgcca ttgaaaaatc aagctgatgc aatgatttac
     3541 gaagttcata ttagagattt ttcagtttct cccaattcag gcataaaaaa taaaggaaaa
     3601 tttctgtcat ttactgaacg cggcactaaa aatcaatttg gcgaagcaac aggcattgat
     3661 catctgaagg aattaggaat tactcatgta catttgttgc ctgtatttga ttttgcatct
     3721 attgatgaaa caaaattatc tgaaaataaa tacaattggg gctatgatcc gttaaattac
     3781 aatgttccag aaggaagtta ttcctccaat ccttatgatc caaccgtaag aattcgtgaa
     3841 ttcaaacaaa tggtacaatc attgcatcaa gcaggaattc gcgtaattat ggatgtggtt
     3901 tacaatcata cttttacagg agaaaattct cacttgaatc ttcttgttcc aggatatttt
     3961 tatcgaatga atcccgacag cacctggtcg aatgctactg gttgtggcaa tgaaaccgct
     4021 tctgaacgtc ccatgatgcg gaaatttatt gtcgaatcag tcgtttattg ggctactgaa
     4081 tatcacattg atggttttcg tttcgatttg atgggcgttc atgatattga aacaatgaat
     4141 gccgtgcgtg aagcacttga cagaatcgat ccttctatta ttgtttatgg cgaaggctgg
     4201 acggcaggtc cttctgttta tccagaatca aaacgagcag tgaaaaaaaa tgcaaaattg
     4261 ctggatagaa ttgccgtttt cagtgatgat attcgagatg ctttgaccgg tagctggatg
     4321 catcccgata ttcctggctt tgtgtcagga aaagacagtc tggaagaaag tgtaaaattt
     4381 ggtattgttg gagcaatttt tcatcctcaa gtggattata ctcaacttat ttattcaaaa
     4441 gaaccatatg ccaaaaatcc cattcaggtg ataaatta
//
LOCUS       DAOTKS010000099         4473 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_16764_length_4473_cov_3.814169, whole genome shotgun sequence.
ACCESSION   DAOTKS010000099 DAOTKS010000000
VERSION     DAOTKS010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16764_length_4473_cov_3.814169"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..2617)
                     /gene="gyrA"
                     /locus_tag="P5236_03000"
     CDS             complement(65..2617)
                     /gene="gyrA"
                     /locus_tag="P5236_03000"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005938733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRU63362.1"
                     /translation="MAEEERIIKVNIDEEMKSSYIDYSMSVIVSRALPDVRDGFKPVH
                     RRVLYAMNELGNTSDKPYKKCARIVGEVMGKYHPHGDSSIYGTLVRLAQPWSMRYPLV
                     DGQGNFGSIDGDSPAAMRYTEARLQKIAEEMLADIDKDTVDFQPNFDETLKEPVVLPS
                     RLPNLLINGSSGIAVGMATNMAPHNLSETIDAIIAYIDNKEITIPELMKYLKAPDFPT
                     GGIIYGYDGVKEAFETGRGRIIIRGKTEIEVDASGHEKIIIKEIPYQINKVELIKIIA
                     SLVEDKKIEGISNINDESDREGMRIVIDVKKDANSNVILNKLFKNTPLQSSFNVNNIA
                     LVNYRPYLLTLKDLIRHFVNHRHTVITRRTKFELSEAEKRAHLLEGFMIILDNLDEAI
                     QIIRDSKNPDEARTRLMERFNLTEVQSRAIVEMRLRQLTGMEQDKLRAEYQEVLQLIT
                     HLKEILEKESLRMQIIKDELLEIKKQYGDPRRTEIVYSSEEFNPEDFYANEQMIITIS
                     HLGYIKRTPLSEFRAQGRGGIGSKGSDSRDEDFIEYIYPASMHNTMLFFTQKGKCYWL
                     KVYEIPEGSKNSKGRAIQNILNIDPDDKVNVFIRVESLSDPDYNQSHYLIFCTKKGVI
                     KKTSLEAYSRPRQNGVIAIQLRENDEVIQVRLTDGNSEIIMANRNGRAIRFHESNVRE
                     MGRTATGVRGMTLDEDGEDEVIGMICVNKDSGENILVVSEQGYGKRTMLDDENGEPVY
                     RITNRGGKGVKTMNITEKTGKLVAIKSVTDENDLMIINKSGITIRLKVSDIPVIGRAT
                     QGVRLINLEKRNDEIASVCKVSSETNENQEFEIDEENEEENLLATSNEFLEN"
     gene            3097..3486
                     /locus_tag="P5236_03005"
     CDS             3097..3486
                     /locus_tag="P5236_03005"
                     /inference="COORDINATES: protein motif:HMM:NF022317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 family protein"
                     /protein_id="HRU63363.1"
                     /translation="MHTVYSILDIFFVIFHTSLTIFNAIGWVWKKTRLLNLITLLLTA
                     SSWLFLGMIVGTPGYCPLTDWHFMVLEKLGKTNLPNSYLKYLFDRITGLNINAELVDK
                     VTLIVFLAALAISIFLNILDKVVLKRK"
     gene            3626..4054
                     /locus_tag="P5236_03010"
     CDS             3626..4054
                     /locus_tag="P5236_03010"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRU63364.1"
                     /translation="MKIRQLSVFVENKTGHLNEILNILARNNINIIALTLADSSDYGL
                     LRMLVSDPLKAVEVLRAENCSVRIHDVLSLELEGVPGSLARILDKFTAENISIEYLYA
                     FSFGSKSIMVMRTNNMDKATEVIKKYQFKPIYESDLKLTV"
     gene            4051..>4473
                     /locus_tag="P5236_03015"
     CDS             4051..>4473
                     /locus_tag="P5236_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HRU63365.1"
                     /translation="MIWNEEVECMSHEEMRELQSERLRNLVNRVYRNVPFYRKKMEEM
                     NITPSDIHSIDDISKLPFTYKNDLRDNYPFGLFAVPMKDIVRVHASSGTTGKPTTVGY
                     TKNDIQNWCESLARCLTMAGVTKEDVVQVSYGYGLFTGG"
BASE COUNT         1331 a          820 c          750 g         1572 t
ORIGIN      
        1 attgaataat ttattagact gtaaaaatac agtagatcaa aagattgaac aaattctttt
       61 aaaattaatt ttctaaaaat tcattagatg ttgctaataa attttcttct tcattttcct
      121 catcaatttc aaattcttgg ttttcattgg tttcagagga aactttgcaa acagaagcta
      181 tttcatcgtt ccgtttttcg aggttgatga ggcgaacacc ttgtgtggca cgaccgataa
      241 caggaatatc agaaactttc aacctgatgg taatgcctga tttattgata atcattaaat
      301 cgttttcgtc agtaacactt ttgatggcta ctaatttacc cgttttttct gtgatattca
      361 ttgttttaac acctttaccg ccgcgattag taattctata aacaggttca ccgttttcat
      421 catccagcat agtccgtttt ccgtagcctt gttctgaaac caccaaaata ttttctccac
      481 tatctttatt cacacaaatc atgccaatta cttcatcctc tccatcttca tcaagtgtca
      541 ttccacgaac accggttgca gtacgtccca tttcacgaac atttgactcg tgaaagcgaa
      601 tggctcgccc atttcgattg gccatgataa tttcagaatt tccatcggtc aatcttacct
      661 gaatgacttc atcgttttcc cttaactgaa tggcaattac accattttgt cgtggacgtg
      721 aataagcttc caaacttgtt tttttgataa cacctttttt tgtgcaaaaa atcaaataat
      781 gggattgatt ataatcagga tcagataggc tttcgacacg aataaacaca tttactttgt
      841 catcaggatc aatgttcagg atattttgaa tggctcttcc tttggaattt ttacttcctt
      901 ctggaatttc gtaaactttc agccagtaac attttccttt ttgggtaaaa aatagcatgg
      961 tgttatgcat ggaagctgga taaatatatt ctataaaatc ttcgtctctt gaatcacttc
     1021 cttttgaacc aatgccgcct cgtccttgag ctcggaattc gcttaatggt gtccgtttga
     1081 tatagccaag atgagaaatg gtaataatca tttgttcatt ggcataaaaa tcttctggat
     1141 taaattcttc agaagaataa acaatttcag tccgacgtgg atcgccatat tgctttttga
     1201 tttcaagaag ctcatcttta ataatttgca tacgcaaaga ttctttttct aatatctctt
     1261 ttaaatgagt tattaattgt agcacttctt gatactctgc tcgcaattta tcttgctcca
     1321 tacccgtaag ttggcgtaat cgcatttcaa caatggctct tgactgaact tccgtaagat
     1381 taaaacgttc cattaaacga gtgcgtgctt catcaggatt ttttgaatca cgaatgattt
     1441 gaatagcttc atccagatta tccaggataa tcataaatcc ttccagtaaa tgtgctcgtt
     1501 tttcagcttc tgacaattca aacttggtac gacgagtaat aaccgtatgg cgatgattta
     1561 caaagtgacg aattaaatct tttaatgtca ataaataagg tcggtaattt actaatgcaa
     1621 tattattgac attgaaagag gattgtaagg gcgtgttttt gaatagttta tttaaaataa
     1681 cattggaatt cgcatccttc ttaacatcaa ttacaatccg catcccttca cggtcagatt
     1741 catcattgat atttgaaatt ccttcaattt tcttgtcttc taccagagaa gcaataattt
     1801 ttatcaactc aactttattt atctgatagg gaatttcttt tataatgatt ttctcatgac
     1861 ctgaagcatc aacttcaatt tcagtttttc cacgaataat aattcgtcct cgtcctgttt
     1921 caaaagcttc cttcacacca tcgtagccat aaatgatgcc acctgtagga aaatcaggag
     1981 ctttgaggta tttcatcaat tctggtatgg taatttcttt attatcaatg taagcaatga
     2041 tagcatcgat agtttcggaa agattgtgag gagccatgtt ggttgccatt ccaacagcaa
     2101 tccctgacga gccatttatt aacagattgg gtaaacgtga tggcaatacc acaggttctt
     2161 tcaatgtttc gtcgaaattc ggttgaaaat caacagtatc tttgtcaatg tcagccaaca
     2221 tttcttcggc tattttttgc aggcgagctt cagtataacg catagcagca ggactgtccc
     2281 cgtcaataga accaaaattg ccttgtccat caaccagcgg ataacgcatt gaccacggtt
     2341 gtgctaaacg aaccagtgtt ccgtaaatgg aagaatcacc atgcggatga tactttccca
     2401 tcacttctcc aacaattctt gcacattttt tataaggttt atcagaagta ttgcccaatt
     2461 cattcattgc atatagcact cttcggtgca cgggcttaaa accgtcacgc acatcaggca
     2521 atgctcttga tacaatcaca gacattgaat aatcaatgta cgaagatttc atttcttcgt
     2581 caatgttcac tttaataatt ctttcttctt cagccattta tattgttttt atatcttctt
     2641 gaaaaattgt actaaattac tcatttttct tcagatgggc aatttttaaa agttaattta
     2701 tggtaagaat tcccttttac agagcactgg gaagtttttt gattttcagt tcaggcaatg
     2761 gaggaaatct aatttgaaat aagatgaggt tacttttgca aataaacagt gaaataaaat
     2821 tcaggcaata ttttttcaat cttattttgc tgaaaaccgt cataacaaat ctggaaattt
     2881 aatttttagt gtaagatggt gaggaaggta caccgaacaa gaggataaat ttgtctaaag
     2941 tatagacgcg gcttttcgaa tttgttcaaa gcgtttgatt aagctgcatt ttttagctaa
     3001 gttttatttc tttcttcatc ttattaaaaa taattttcag ctccgtggtt tcaactttta
     3061 aaaattgctg tacttttgtt gtacaagaaa ttttttatgc ataccgtcta ttccatactg
     3121 gatattttct tcgtcatttt tcatacctca ctaactattt tcaatgccat tggttgggta
     3181 tggaagaaaa ctcgattgct taatctgatc acgcttcttc ttacggcctc ttcgtggcta
     3241 tttctgggaa tgattgtcgg tacaccgggt tattgtccac tcaccgactg gcattttatg
     3301 gtactggaaa aactggggaa aacaaatctt ccaaattctt atctcaaata cctttttgat
     3361 agaattacag ggctgaatat taatgctgaa ctggtcgaca aggtgacatt aattgtgttt
     3421 cttgcagctt tagcaatttc tatttttctc aacattctgg ataaagttgt tttaaaacgc
     3481 aaataatatt ttgcatttgc cattactcgt gtcaaaactt tgacgagtcg atacacttcg
     3541 ccaaataagc tatctaaatt tatgctgttt atttttatta tttgttgtaa atttgcattt
     3601 ttaaattttt aaaattattt ctgcaatgaa aatccgtcaa ctttctgttt ttgtggagaa
     3661 caaaactggt catctcaacg aaattttaaa tattttggct cgcaataata tcaacattat
     3721 tgccttaaca cttgccgata gcagcgatta tggcctttta cgtatgttgg tgtccgatcc
     3781 gttaaaagca gtagaagtac tcagagctga aaattgttct gtccgcattc acgacgttct
     3841 ttctttggaa ttggaaggtg ttcctggctc attagctcgc atccttgata aatttacggc
     3901 tgaaaacatt tctattgaat atctttatgc ttttagtttt ggaagcaaat ccattatggt
     3961 aatgcgaacc aacaatatgg ataaggcaac agaagtaatt aaaaaatatc aattcaaacc
     4021 catttatgaa tcagatttaa aactgacggt atgatctgga atgaggaagt agagtgtatg
     4081 tcgcacgaag aaatgcgaga acttcaaagt gaacgtttaa gaaatttagt gaacagagta
     4141 tatcgaaatg tgccttttta tcgcaaaaaa atggaggaaa tgaatattac tccctctgat
     4201 attcactcca ttgatgatat ttccaaactg ccttttactt ataaaaacga tttacgtgac
     4261 aattatccat ttggactttt tgctgtccca atgaaagata ttgttagagt acatgcttcc
     4321 agtggcacaa caggtaaacc taccacggta ggttatacaa aaaatgatat tcaaaattgg
     4381 tgtgaatcac tggctcgttg tttaacgatg gctggggtta caaaagaaga tgtggtgcaa
     4441 gtttcttatg gatatggttt gtttacaggt ggc
//
LOCUS       DAOTKS010000100         4465 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_16801_length_4465_cov_4.578685, whole genome shotgun sequence.
ACCESSION   DAOTKS010000100 DAOTKS010000000
VERSION     DAOTKS010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16801_length_4465_cov_4.578685"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..1314)
                     /locus_tag="P5236_03020"
     CDS             complement(82..1314)
                     /locus_tag="P5236_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005467366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HRU63366.1"
                     /translation="MKNLKLKIGFLLFFFVFLFPVTIFAQSNEGGNLIVQSIQKFWLN
                     TGIANFEFKYLAMIIVGIFFIYLGIAKEWEPLLLVPIGFGILIGNIPFMEGYEIGIYE
                     EGSVMNLLYSGVIQGWYPPLIFLGIGAMTDFSSLISNPKLMLLGAAAQLGIFLTFIGA
                     YALGFTPAQAGAIGIIGGADGPTAIFLSSRLAPELLGAIAVAAYSYMALVPVLQPPVM
                     RLFTTSKERVIRMKPPRQVTKLEKIAFPIVGFLLTTFISPSAIPLLGMLFLGNLLKES
                     GVTERLADTARNALINIVTILLGLTVGASTQADRFLTVDSLLIFGLGALSFMIATAGG
                     VLFAKIMNLFLKDGNKINPLIGSAGVSAVPLSAHVSQSIGNSYDKNNHLLMHAMGPNV
                     AGVIGSAVAAGILLSILS"
     gene            complement(1327..1773)
                     /locus_tag="P5236_03025"
     CDS             complement(1327..1773)
                     /locus_tag="P5236_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRU63367.1"
                     /translation="MKKFDFTIHGNEYHVLIKSVEGNLVELEVNGTPYTVTLNQEVKV
                     SKTPILVRKEIPATPSNMNVAEKFNPVPSTDKPSARVIKSPLPGNVIKVNVSVGDIVK
                     EGDVLMVLESMKMENNILSEKSGKITKVCVTPGKSVLQDEILFEIE"
     gene            complement(1773..2114)
                     /locus_tag="P5236_03030"
     CDS             complement(1773..2114)
                     /locus_tag="P5236_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HRU63368.1"
                     /translation="MKLLAINSWEELLMGAAIGIIVVFMVLTILVLLFEFIQKMVISM
                     TKKKVVKEGKKIEEIKDIPANDIAAISLALHLYFNSLHDEENHVLTIKRIERRYSPWN
                     SKIYGLTNLHR"
     gene            complement(2150..>2428)
                     /locus_tag="P5236_03035"
     CDS             complement(2150..>2428)
                     /locus_tag="P5236_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRU63369.1"
                     /translation="EIAVMGASGAIEILEGRKIATITDPEEKEKYIAKKVEEYNDTFA
                     NPYQAASYGYIDDVIEPRDTRFRVIRALETLQTKKVVNPLKKHCNIPL"
     assembly_gap    2429..2528
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2529..3457)
                     /locus_tag="P5236_03040"
     CDS             complement(<2529..3457)
                     /locus_tag="P5236_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRU63370.1"
                     /translation="MNNQEKINYLLELRAKAKLGGGEKRIAKQHAKGKYTARERIEML
                     LDDASFEELDMFVTHRCTNFGMEKEKYLGDGVVTGHGTIDGRTVYVYAQDFTVFGGSL
                     SETMALKICKVMDMAMKMGAPIIGINDSGGARIQEGVTSLAGYAEIFERNILASGLIP
                     QISAIFGPCAGGAVYSPALTDFILMTEQNSYMFVTGPKVIKSVTGEDISVEDLGGANV
                     HSSKSGVSHFKVETEEEGLLLIRKLLSYLPQNNLEEPPLVENFDPIDRVDDKLNTIVP
                     DNPNQPYDMKEIIRSIIDNGEFLEVHQQYARNI"
     gene            complement(3516..3926)
                     /gene="mce"
                     /locus_tag="P5236_03045"
     CDS             complement(3516..3926)
                     /gene="mce"
                     /locus_tag="P5236_03045"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HRU63371.1"
                     /translation="MELKKIEHIGIAVKSLEVSIPFYENVLGLKCYAIEEVADQKVKT
                     AFFKVGETKIELLESTDPAGAIGKFIENKGEGVHHVAFLVDNVQKALDEASEKGIRLI
                     DNQPRKGAEELNIAFLHPKSTGGILIELCEQPGK"
     gene            complement(4186..>4465)
                     /locus_tag="P5236_03050"
     CDS             complement(4186..>4465)
                     /locus_tag="P5236_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63372.1"
                     /translation="FDLQTELLNTIRQEFAHNLSQQIYVSNELWNYIRTAEESLLRLV
                     NMCASQCNPADPANVLAEKIIEVYNASEHTPTEQAIEKLKAEVRSYFQ"
BASE COUNT         1463 a          791 c          633 g         1478 t
ORIGIN      
        1 aaacttcatt taaaatgaca gattaaaata caaaagccca ctgaaaccaa tgagcttaaa
       61 atagaaataa tgataaaatt gtcaacttaa aatacttaac aaaataccag cagctactgc
      121 tgaaccaatt acacctgcca catttggtcc cattgcatgc atcaataagt gattgttttt
      181 atcgtatgaa tttcctatag attgagaaac atgagcagaa agaggaacag ccgatactcc
      241 tgctgatcca attaaaggat taattttatt gccatctttt agaaataaat tcataatttt
      301 agcaaacaat acacctcctg ctgtggcaat cataaatgat aaagctccca aaccaaagat
      361 taataaggaa tcgacagtta aaaagcggtc tgcttgggta gaagctccta cggttaatcc
      421 taacaaaata gtcacaatat taattaatgc atttcgtgca gtatcagcta aacgttctgt
      481 tactcctgat tcttttaaaa gattacctaa aaacaacatt cccaaaagag gaatagcact
      541 tggagaaatg aaagtggtta ataaaaaacc aacaatgggg aaagctattt tttctaattt
      601 tgttacttga cgaggaggtt tcattctgat aactctttct ttagaagtag taaatagtct
      661 cattacaggt ggttgaagca cgggcaccaa tgccatataa gaataagcag ccactgcaat
      721 tgcacccaac aattcagggg ctaacctcga agataaaaag atggctgtag gtccatcagc
      781 accaccaata attccgatag cgcctgcttg cgcaggagta aaacctaaag cgtaagcacc
      841 aataaaagta agaaaaatgc ctagttgagc agcggcaccc aataacatta atttaggatt
      901 ggaaatcagt gaagagaaat cagtcatagc accaatgcct aaaaaaatga gtggaggata
      961 ccatccttga ataacaccgc tatatagtaa attcattaca gaaccttctt cataaattcc
     1021 tatttcatag ccttccataa aagggatatt accaattaaa attccaaacc cgataggtac
     1081 aagtaaaagt ggttcccatt ctttggcaat tccaagataa atgaagaaaa tccctactat
     1141 aatcattgcc aggtacttaa attcaaaatt tgctattcct gtgttcaacc aaaatttttg
     1201 aatactttga acaatcaagt ttcccccttc attggactga gcaaaaatgg taacagggaa
     1261 taaaaaaaca aaaaagaaaa gtaaaaatcc tattttaagc tttaaatttt tcattgatat
     1321 tcaaatttat tctatttcaa ataaaatttc atcttgtaat acagatttac ctggtgtaac
     1381 acatactttt gttatcttac cacttttttc agaaagaata ttattttcca ttttcattga
     1441 ttcaagcacc attaatacat ctccttcttt aacaatatct cctactgaaa catttacttt
     1501 tataacattt ccaggaagag gtgatttaat aactcttgca ctgggtttat cggtagatgg
     1561 tactggatta aatttttcag ccacattcat attacttgga gtagcaggaa tttcttttct
     1621 taccagaatg ggtgtcttgc taacttttac ttcttgattg agagtaactg tatagggtgt
     1681 accattaact tcaagttcaa ctaaatttcc ttccacggat tttattaata cgtgatattc
     1741 attaccatgg atagtaaaat caaatttttt cattatctat gcaaatttgt aagtccataa
     1801 atttttgaat tccatggtga ataacgacgt tcaatacgct taatggtaag aacgtgattt
     1861 tcttcatcat gtaatgagtt gaaataaaga tgtaaagcta aagaaatggc agcaatgtca
     1921 ttagctggaa tatctttgat ttcttctatc tttttccctt cttttactac ttttttcttg
     1981 gtcattgaaa taaccatctt ttgaataaat tcaaatagaa gtactaaaat agttaaaacc
     2041 ataaaaacta ctataattcc aatagcagct cccatcaaaa gttcttccca cgaattgata
     2101 gccagtaatt tcatatatta agttattatt tattagtaaa ctaaatattt tacaatggga
     2161 tattgcaatg tttttttaag ggatttacca cttttttagt ttgtaaagtt tctaaagcgc
     2221 gaatgactct gaatcgggta tcgcgtggtt caatcacatc gtcaatatag ccataagaag
     2281 cagcttgata aggatttgca aaagtatcat tgtattcctc tacttttttt gcaatatatt
     2341 tttctttttc ttcaggatca gtaattgttg caattttacg tccttccaat atttcaatgg
     2401 ctcctgaagc acccattaca gcaatttcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnat aatatttcga gcatattgtt gatgaacttc taaaaattca ccgttatcta
     2581 tgatagatcg aataatctct ttcatatcgt aaggttgatt gggattatcg ggtacaattg
     2641 tatttaattt atcatctacc cgatcaattg gatcaaaatt ttctactaaa ggcggttctt
     2701 ccagattatt ttgaggcaag taggataaaa gctttcgaat aagcagtaaa ccttcttctt
     2761 ctgtttctac tttgaaatga gaaactcctg attttgaaga atgtacattt gctcctccta
     2821 aatcttccac ggaaatatct tctccagtaa ctgactttat gactttggga ccagttacaa
     2881 acatgtaact attttgttcg gtcattaaaa taaaatcagt caatgctggt gaatatacag
     2941 cacctccagc acaaggtcca aaaatagctg atatttgcgg tattaaacct gaagctaaaa
     3001 tgtttcgttc aaaaatttct gcataaccgg caagtgaagt aactccttct tgtattcgag
     3061 caccgccaga atcattgata ccaataatag gagctcccat tttcatagcc atatccatca
     3121 ctttacaaat tttgagagcc atcgtttcag aaagcgaacc gccaaatacg gtaaaatcct
     3181 gagcgtaaac ataaacagtg cgaccatcaa tagtcccatg accagtcact acaccatcgc
     3241 ccagatattt ttctttttcc attccaaaat tcgtgcaacg atgagtgaca aacatatcca
     3301 gctcttcaaa acttgcatca tccagcaaca tttcgatacg ttctcttgct gtatattttc
     3361 ctttagcgtg ttgtttagct attctttttt ctccacctcc caactttgcc tttgcacgaa
     3421 gttcaagaag ataattgatt ttttcctgat tgttcatctt attttataat taattgattc
     3481 ccattttaaa aaacttctaa aaataatcct taaaattatt tgcctggctg ttcgcaaagt
     3541 tcaatcaaaa ttccccctgt tgatttggga tgtagaaagg caatgtttaa ttcttctgct
     3601 ccttttcgtg gttgattatc gataagcctg ataccttttt cggaagcttc atccagtgct
     3661 ttttgtacat tatccactaa aaaagcaaca tgatgaacac cttctccttt gttttcaata
     3721 aatttcccta tcgctcctgc tggatcggtt gattccaata attcaatttt tgtttccccc
     3781 actttaaaaa aagctgtttt tactttctga tcagctactt cttctattgc ataacacttt
     3841 aatcctaata cattttcata aaaaggaata gatacctcta aactttttac tgctatgccg
     3901 atatgttcta ttttttttag ttccataaat atataattta ggttgataga taattttact
     3961 aaaatattca aatttttaat tcctttaata aaagaaaaag ttgaattaaa gaaatatttc
     4021 agtatttaat ttttgcacaa aattaataaa tataattcat ttatgcatta gaaaacacag
     4081 atgaatttgt attttttaaa aatgattgag tgtcaattac actaaaagcc tataactttt
     4141 attgggaagt gaaatttaaa agaaaatagt taaaaaaact attttttatt gaaaataact
     4201 tcttacttca gcttttaatt tttcgatggc ttgttcggta ggagtatgtt cactggcatt
     4261 ataaacttca atgatttttt ctgccaaaac attcgcggga tcagcaggat tacattgcga
     4321 agcacacatg tttactaatc gaagtaaact ttcttcagca gtccgaatgt aattccataa
     4381 ttcattacta acataaattt gttgtgaaag attgtgggca aattcctgcc tgatagtatt
     4441 taataattct gtttgaagat cgaaa
//
LOCUS       DAOTKS010000101         4454 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_16849_length_4454_cov_3.935213, whole genome shotgun sequence.
ACCESSION   DAOTKS010000101 DAOTKS010000000
VERSION     DAOTKS010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16849_length_4454_cov_3.935213"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1765)
                     /locus_tag="P5236_03055"
     CDS             complement(<1..1765)
                     /locus_tag="P5236_03055"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HRU63373.1"
                     /translation="MKNKNLSKLEFRGLKVFVLLGMLSCALSMSAQINIQGKVIDQNN
                     EPLTGVTVMIKGTSIGTITDINGNFSLSVPDQKAVLVFSFVGYQKQEVPVGTSRQLTI
                     TMIEEPKLLQEVVVVGYGTQKKVSVTGSISQISGAEMLKAPPVNLSQMLGGRVTGLVT
                     RQSSGIPGSDDASMVIRGITTTSGATDPMIIIDGVPRSFNQLDPNEIESISILKDAAA
                     AAVYGIQGANGVILVTTKKGMEMKPQITYSGNFEINQNTSFPKFMNGPQYAYYWNKAL
                     EMDEQAPVFTEEMVNKMINGDPEGVYGNTNWVDEIFKTGQTQHHNLSATGGTKDVKYY
                     MMIGYYDQKGNVKNFDFNRYNVRSNIDANIVKGLTANFNIGARKENRNRPYFGAGKNE
                     YMSIIQQAIRAHPYVPLTYNGLPTATKTASAQVSPVAARDLSGFNDSEMSVFQSNFAL
                     NWEIPWLKGLSARAMGSFDRDYTHSKVFKTPFQVSLASRPTPTYPSLKYETMYPVAIG
                     NVATMSEGFAEATRITFQGQINYAQTFGKHDISALALWEQSNYIYKQFNVNVKDFDFY
                     DFAELNHAKSIADQNQFSGSSK"
     gene            complement(1846..3261)
                     /locus_tag="P5236_03060"
     CDS             complement(1846..3261)
                     /locus_tag="P5236_03060"
                     /inference="COORDINATES: protein motif:HMM:NF027716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HRU63374.1"
                     /translation="MKRAVALDVLRGISIFGMVLSGNIPFGVLPAWMYHRQTPPPSHV
                     FNPDVPGITWVDLVLPIFIFCMGVAIPLALNKKIEAGKNFWNIAQSIIKRYFMLVIFA
                     ILIAHFQTSAIPKSFVNFHFLGIYIKGYDLFLITIIGFLSLFSIYVIIENQQQKKKWR
                     IIGWTAIVSLLLLLHYVYGLKFSLHRSNIIILLLANVYLFGAFSWYFTRSDKLNRLLL
                     LMIWGAIQITCKTTHFDEALNQISGLSWLFLFRMTHYMLLIIPATIVGDIIYERLTAE
                     NSKNTSHSKKKGEKFISHLIFLLGIWFIVALYNRWLVILYISTPIVLACFYYIVKKYS
                     PSSLSFFKIAAYMILVGLILEPAEGGIKKDPVTASYLFITGGISILWLIFLDYVCQRN
                     EKSHFVKLFAGAGSNPLLAYIATNWFVIPLMNLTFLIIPYQGCYPDGYHWLGVLRAFI
                     LVFCVMFLVALATKKKIRWRA"
     gene            complement(3417..>4454)
                     /locus_tag="P5236_03065"
     CDS             complement(3417..>4454)
                     /locus_tag="P5236_03065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRU63375.1"
                     /translation="FVYNATTGDSICALNTANIVDGLFPLNDVGATDDGKIITASLGA
                     NTNFKIYMYDNLTAEPVVVLHYPDAAPGGRTGDLFTVVGDYSEGTAKIYTATAANPSD
                     IYVLEMENGQWKTERTLVKSISLDGNAVCPGIGVKPNGDLFWKAGGKKIHLITNEAVL
                     SEPEQTISMYSNSVKYIGYSSTYNLDYIVLFTYGGQKECAEIISLTPGDLSTCQIIAR
                     TPSLGANANVGGTGDVEVRYDQQGNPIIYVVSTNNGFGAYKVEGLDITTGLPMPPEKI
                     EVNIYPNPAKEKVHFSQTAKKVKLFSISGQLLKEANNVDHLNVKELKGIYLLEIESQR
                     GEKVFKRLIVE"
BASE COUNT         1463 a          793 c          679 g         1519 t
ORIGIN      
        1 ttttgctgga accactaaat tgattttgat ctgcaattga tttagcatga tttagctctg
       61 caaaatcata aaaatcaaaa tcttttacat ttacattaaa ttgcttatat atgtaattag
      121 attgttccca caatgctaaa gcactaatat catgtttccc aaaagtttga gcataattaa
      181 tttgtccctg gaaagtaatt cgtgtagcct cagcaaaacc ttcggacata gtagccacgt
      241 tgccaattgc tactggatac atggtttcat attttaatga aggataagtt ggcgtagggc
      301 gagaagctaa acttacttga aaaggggttt taaaaacctt gctatgagtg taatctctgt
      361 caaagctacc cattgctctt gctgataacc cttttaacca tggaatttcc caatttaatg
      421 caaagttact ttgaaaaaca gacatttcac tgtcattaaa accagataag tctctcgcag
      481 cgaccggact tacttgagca cttgcagtct ttgtggcagt aggcaaacca ttataagtaa
      541 gaggaacata aggatgagct ctgatagctt gttgaataat gctcatatac tcattttttc
      601 ctgcaccaaa atagggccta ttacgatttt ctttacgggc accgatattg aaatttgcgg
      661 tcaaaccttt tactatgttt gcatcaatat tagaacgaac attataacga ttaaaatcaa
      721 agttttttac gtttcctttt tgatcatagt atcctatcat catgtaatat ttaacgtctt
      781 ttgttccccc tgttgctgaa aggttatgat gttgagtttg acccgtttta aaaatttcat
      841 ccacccaatt ggtattacca taaacacctt cgggatcgcc attgatcatt ttattaacca
      901 tttcttctgt aaatactgga gcctgttcat ccatttctaa tgctttgttc caataataag
      961 catattgagg tccattcata aattttggga aagaagtgtt ttgatttatt tcaaaattac
     1021 cagaataagt aatttgaggt ttcatttcca ttcctttttt ggtggtaact aaaataactc
     1081 cattagcccc ttgtattcca tatacagccg ctgctgcagc atcttttaat atggaaatac
     1141 tttcaatttc gtttggatct aactggttaa atgaacgcgg tactccatct ataataatca
     1201 taggatcagt agcacccgaa gtagttgtaa taccacgaat aaccattgaa gcatcatctg
     1261 acccaggaat acctgatgac tggcgtgtta ccaatcctgt tactcgtccc cctaacattt
     1321 gagaaagatt gacaggaggc gcttttaaca tttcggcacc tgaaatttgg gaaatagaac
     1381 ctgttacact tacttttttt tgtgtgccgt atccaaccac aactacttct tgaagaagtt
     1441 tgggttcttc tatcatggta attgtcaatt gtcggcttgt tcctacggga acttcttgct
     1501 tttgatagcc aacaaatgaa aagactaaaa cggctttttg atcaggaact gataaggaaa
     1561 aatttccatt tatatctgtt attgtcccaa tactagttcc ttttatcatt accgttacac
     1621 ccgttagagg ttcattgttt tgatcaatga cctttccttg aatgtttatt tgtgcactca
     1681 ttgataaagc acaacttaac attcctaaga gcacgaaaac ttttaagcct cgaaactcta
     1741 attttgatag attcttgttt ttcatagaat aaattaatgt ttaatgtaga aagttaatta
     1801 atcacttgtt ttttaaagct atttttttaa aattatttta tctatttaag ccctccatct
     1861 tatttttttc ttcgtggcta aagcaacgag aaacataaca caaaatacta aaataaaagc
     1921 ccttaaaaca cctaaccaat gatatccatc aggataacat ccttgataag gtatgattaa
     1981 aaatgtaagg ttcattaaag gaattacgaa ccaattggta gctatgtatg ctaaaagtgg
     2041 attacttcct gctcctgcaa aaagtttgac aaaatgtgat ttctcatttc tttgacaaac
     2101 gtaatctaag aaaattaacc ataatatact gattcctcct gttataaaaa gataagatgc
     2161 tgtaacaggg tcttttttta ttcctccttc agcaggttct aaaattaatc caactaaaat
     2221 catataggct gctattttaa aaaaggaaag agaagaaggg gaatattttt ttactatgta
     2281 ataaaaacat gccaacacaa taggtgttga aatataaaga attactaacc atcgattgta
     2341 taaagctact ataaaccata ttcctaataa aaaaatcaag tgagaaataa atttttctcc
     2401 tttctttttt gaatgagaag tattttttga attttctgct gttagtcgct cataaattat
     2461 atcgcccact atagttgccg gaatgattaa taacatataa tgagtcattc tgaaaaggaa
     2521 taaccacgac aaacctgaaa tttgattaag ggcttcatca aaatgagtag ttttgcaagt
     2581 aatttgtata gcaccccaga tcattaacag tagcaaacga tttaatttat cacttcttgt
     2641 aaaataccaa ctaaaagctc caaacaaata aacgtttgct aaaagaagaa taataatgtt
     2701 acttctatgt aaagaaaatt ttaatccata cacataatgc agaagtaata ataatgaaac
     2761 gatagcagtc catcctatta ttctccattt ctttttttgc tgttgatttt ctataattac
     2821 ataaatacta aataaagaaa gaaaccctat tatagtgata aggaataaat catatccttt
     2881 aatataaatc cctaaaaagt gaaaattaac aaaactcttt ggaatagcag atgtttgaaa
     2941 atgagcaatc agaatagcaa atattaccaa cataaaataa cgctttataa tactttgagc
     3001 aatattccag aaatttttgc cagcttctat ttttttattt aatgctaatg gaatggcaac
     3061 ccccatacaa aaaataaaaa taggaagaac taagtcaacc caagtaatgc ctggtacatc
     3121 agggttgaaa acatgagaag gtggaggtgt ttgtctatga tacatccatg caggtaatac
     3181 tccaaaaggg atattaccag aaagaaccat cccaaaaata gaaattcctc gtaagacatc
     3241 taaagcgact gctcttttca tgagacaagt tagtattaga ttaaataagg caaggcagta
     3301 attttttgat gatttgttta aattttttag taataatttt ctctttttga aatatatttt
     3361 tatcaaaagc actttttata aggatttcct tataaaaagt gctttacaaa gttgatttat
     3421 tcaactatta aacgtttgaa taccttttct cctctttgag attctatttc taacagataa
     3481 atacctttta attcttttac attgagatga tcaacattat tggcttcttt aagcaattgt
     3541 cctgaaatgc taaataattt tacttttttt gcggtttgtg aaaaatgaac tttctcttta
     3601 gctggattgg gataaatatt cacttctatc ttttcaggtg gcatgggtaa tcctgttgta
     3661 atatctaaac cttctacttt gtatgctcca aatccattgt ttgtggaaac gacataaata
     3721 ataggattcc cttgttgatc ataacgaact tctacatctc ctgtgcctcc tacatttgca
     3781 ttagctccta agcttggagt acgagcaata atttggcagg tactaaggtc acccggagtc
     3841 aaagaaataa tttcagcaca ttctttttgt cctccgtagg taaataatac tatataatct
     3901 aaattatatg tagaactata tcctatgtat tttactgaat ttgaatacat agagatagtt
     3961 tgttctggct ctgacaaaac ggcctcatta gttattaaat gaattttttt cccgcctgct
     4021 ttccagaata aatctccatt gggtttaacg ccgatacctg gacatactgc gttcccatcg
     4081 agtgaaatac ttttgacaag agttctttca gttttccatt gaccattttc catctctaaa
     4141 acataaatgt cagaggggtt tgctgcagta gcagtataaa tttttgccgt tccttctgaa
     4201 tagtcaccta ctactgtaaa taaatctcct gttcgaccac ctggcgctgc atccggataa
     4261 tgcaatacta ctactggctc agcagttaag ttatcgtaca tataaatttt aaagtttgtg
     4321 ttagccccta agctggcagt aataattttc ccatcatcag ttgctcctac atcatttagt
     4381 gggaaaagtc catcaacaat atttgcagta ttcagagcac agatagaatc acctgtcgta
     4441 gcattgtata caaa
//
LOCUS       DAOTKS010000102         4446 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_16888_length_4446_cov_4.037349, whole genome shotgun sequence.
ACCESSION   DAOTKS010000102 DAOTKS010000000
VERSION     DAOTKS010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16888_length_4446_cov_4.037349"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /gene="mutL"
                     /locus_tag="P5236_03070"
     CDS             complement(<1..353)
                     /gene="mutL"
                     /locus_tag="P5236_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRU63376.1"
                     /translation="MSDIIHLLPDSVANQIAAGEVIQRPSSALKELIENSIDAEADTI
                     SIQIKHAGRTLIQVVDNGKGMSETDARMAFERHATSKISEINDLYSLHSLGFRGEALA
                     SIAAVAQVELKTRRA"
     gene            complement(367..648)
                     /locus_tag="P5236_03075"
     CDS             complement(367..648)
                     /locus_tag="P5236_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63377.1"
                     /translation="MITSFFNIYKPRKYDYRYIYYDPLKEARKERERRMGVTNEDSDK
                     EYRPTLHRGSFREEADKLKKTCTKEVRKTNRNVLIILIILLILAYFLLR"
     gene            complement(680..1753)
                     /locus_tag="P5236_03080"
     CDS             complement(680..1753)
                     /locus_tag="P5236_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HRU63378.1"
                     /translation="MEKNFCIIMAGGIGSRFWPFSRNNRPKQFLDFFGTGRSLLQLTF
                     DRFKKVVPIENILIVSNVIYKDLILEQLPEIQENQLLLEPVRRNTAPCIAYAVNRIKT
                     ITDEANIIVTPSDHLILKEDEFLATIQEGLQFIEKNDCLLTLGIKPSRPETGYGYIQI
                     EPGDGKLRKVKTFTEKPNAELAKIFFESGEFFWNAGIFMWNLRTILAALDFHIPDLAI
                     KFKTIRKYLNTPEEQKYIEEIYPTCPNISIDYGIMEKALNVYVLCSDFGWSDLGTWGS
                     LYEMSPKDKDGNVSLKGKTIFYESNDNIVALTPDKLAVIQDLHGYIVADSDNVLLICK
                     RENEEQIRQFVKDAQILFDGKYN"
     gene            complement(1883..3259)
                     /locus_tag="P5236_03085"
     CDS             complement(1883..3259)
                     /locus_tag="P5236_03085"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRU63379.1"
                     /translation="MKIKENNWLLTAIVIGSFFNSFMSSSLNIAMPVIDAEYHVGLAE
                     VAWISKSFLLASAVGLLLAGWLADQFGRRKIFLIGNILFGVVSLAIILAENFYIEVAL
                     RFIQGLGSSMIMASSPALIASAFPSSRRGFTLGIQTTFTYMGLSLAPLLGGLMTQTLG
                     WKSLFMLNFIFTVLVIIIMLFNIHHEWKNEEISSFDLKGYLLFVVSMISVVFALEWIN
                     WKSIVALCIAGIVGYFFVSYELRIDQPFISIPLLKNNKYFTHSVLAALINYATTFAIS
                     YVLSLYLQRIRGISPFKSGLILCFQPLMMALFSAYSGHLSDRHNPGKIASYGMMIIAI
                     SLFFIGLLLSKTLPLYVLAIVLAVLGFGFALFSSPNTNAVMSSVDTNSYGFASAFLAF
                     NRLFGQFISMALSAALITLFSRQNVSPVPQHSLLIACKIIFIIFAILSCYGVYLSSQR
                     IHSKINSK"
     gene            complement(4053..>4446)
                     /locus_tag="P5236_03090"
     CDS             complement(4053..>4446)
                     /locus_tag="P5236_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013064429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63380.1"
                     /translation="ITPRKLRDENSILKRKDLRLVQARDAIPATSNQILQGITRAALQ
                     TTSFMSAASFQETTKVLTEAAINGKVDKLEGLKENVICGHLIPAGTGQRDFEKIIVYS
                     REEYEKKTFARKNVLDMENEELVFEEEE"
BASE COUNT         1579 a          778 c          627 g         1462 t
ORIGIN      
        1 tcggcacgac gtgttttcag ttctacttgt gctactgctg caatggaagc taaagcttct
       61 ccacgaaaac ctaacgaatg taaagaatat aaatcgttta tttctgaaat tttggaagta
      121 gcatgccgtt caaaagccat tcgagcatcg gtttcactca ttccctttcc attatcaacc
      181 acctggatca aagtgcgtcc agcatgtttg atttgaatac taatcgtatc agcctcagca
      241 tcaatagaat tttctatcaa ctccttcaat gcggacgatg gacgttgaat aacctcacct
      301 gcagcaatct gattggcaac tgaatcaggt agtaaatgaa taatatcact catgcagaag
      361 ataaaattac ctcaacaaga agtaagcaag aattaataaa ataatcaaaa tgattaacac
      421 atttcgattg gttttacgaa cttcttttgt acatgttttt tttaatttgt cagcttcttc
      481 acgaaacgaa ccacgatgca gagtaggtcg gtattcttta tcactatcct cattagtgac
      541 acccattctt cgttctcttt ctttacgggc ttctttcaga ggatcataat agatatagcg
      601 ataatcatat ttacgcggtt tgtaaatatt aaaaaaactt gtaatcatag ttagaaaaat
      661 cagttattaa acgaatcaat taattgtatt taccatcaaa taaaatttgt gcatctttta
      721 caaattgacg aatttgttct tcgttttcac gtttgcaaat caataataca ttatcggaat
      781 cagcaacaat atacccatgt aaatcttgaa tgactgctaa tttatcagga gtaagagcta
      841 caatgttatc attactttca taaaagatag ttttaccttt taagctcaca ttaccatctt
      901 tatctttggg tgacatttca taaagtgaac cccatgttcc cagatcactc catccaaaat
      961 ctgaacataa tacataaaca tttaaagcct tttccattat gccataatca atagaaatat
     1021 taggacacgt tggatatatt tcttcaatat acttttgctc ttcaggagta ttgagatatt
     1081 ttcttattgt tttaaattta attgccagat cggggatatg aaaatctaag gctgctaaga
     1141 tagttctcaa attccacata aaaatacccg cattccaaaa aaattctcct gattcaaaaa
     1201 aaatctttgc taattcagca ttcggttttt cagtgaatgt tttcactttt cttaatttac
     1261 catcaccagg ttctatttga atatagccat aacctgtttc aggacgactg ggcttaattc
     1321 ccaaagttag taaacaatcg ttcttttcaa taaactgaag tccttcctga atagttgcta
     1381 aaaattcatc ttcttttaaa attaaatggt ctgaaggagt tacaataata ttggcttcgt
     1441 cagttattgt ttttatacgg tttacagcat aagcaataca cggtgccgta ttcctcctta
     1501 ctggttctaa caacaattga ttttcttgta tttcaggaag ctgctctaaa attaaatctt
     1561 tataaataac attagaaaca atcaagatgt tttcaattgg caccactttt ttaaatctat
     1621 caaaagttag ttgtaataag gatcgtccgg ttccaaaaaa atctaaaaat tgtttgggtc
     1681 tgttatttct gctaaaaggc cagaaacggc ttccaatacc gcccgccatg ataatacaaa
     1741 aatttttttc cataatattt ttgaataaag ccgttaatta ttaatgccat tcaaatagca
     1801 ctactaagat acagtgtttt tcttttcttg ccaaaactta gaatctaaaa ataagttttt
     1861 aaaaacaaat gataacaaaa aactattttg aatttatctt tgaatgaatt cgttgagatg
     1921 ataaatagac accataacag cttaaaatag caaaaatgat aaaaataatt ttgcaagcta
     1981 ttagtaatga atgttgagga acaggagaaa cgttttgcct gctaaataat gttatcaaag
     2041 ctgctgaaag agccatcgat ataaattgtc caaacaatcg attaaaagcg agaaaagcag
     2101 aagcaaaacc gtaagaattt gtgtcgacag aactcattac ggcattggta ttgggcgaac
     2161 taaacaaggc aaatccaaaa cctagcacag ctaatacaat tgccagcaca tataatggca
     2221 atgttttact aagaagaaga cctatgaaaa atagtgaaat tgcaataatc atcataccat
     2281 aagaggctat tttgcctgga ttatgccgat cggataaatg tcctgaataa gcagaaaata
     2341 aagccatcat caaaggctgg aaacataaaa tcaaaccaga tttgaaagga gaaatacctc
     2401 gaattctttg taaatataga cttaaaacat aacttatagc aaaagtcgtt gcatagttta
     2461 ttaaggcagc caaaacagaa tgagtaaaat acttattgtt tttaagtaaa gggatagaaa
     2521 taaaaggttg atctattcga agttcgtaag aaacaaaaaa ataaccgact attccagcaa
     2581 tacataaagc aactatcgat ttccaattta tccattctaa tgcaaaaact acacttatca
     2641 tcgatacaac gaatagcaaa tatcctttca gatcgaagga agaaatttct tcatttttcc
     2701 attcatgatg aatattaaaa agcattataa tgataactaa tacagtgaaa ataaaattca
     2761 acataaaaag gcttttccag cctaatgtct gcgtcattaa tccacccagc aatggagcaa
     2821 gtgaaagccc catataagta aaagtagttt gaatacccaa tgtaaatcct cttcgggaag
     2881 aagggaatgc tgaagcaata agagcaggcg aagaagccat aatcatagaa ctgcccaaac
     2941 cttgaataaa tcgcaaggca acttcgatat aaaaattttc agctaaaata attgccagtg
     3001 aaacaactcc aaaaaggatg ttcccaatga gaaatatttt tcgtcgtcca aactgatcag
     3061 ccaaccaacc tgccaaaagt aatcctactg ctgaagccaa aagaaacgat tttgaaatcc
     3121 atgccacttc cgctaagcca acatgatatt ctgcatcaat aactggcatg gcaatattta
     3181 aagaagagga cataaaacta ttaaaaaaag aacctattac aatagcagta agcaaccaat
     3241 tattttcttt gattttcatt gccaagcaat ttttaactta tataaatttc gatttataat
     3301 tacacaaatt attagttaga aagaaaatat tgcccataaa tacttgatat tttctttttt
     3361 cttgataaac aataaaaaat ttgtgccatc aactttcaaa atcaattcaa taaagtaata
     3421 aggcctggtt tgtttgttta tttcttgtta tgaaggtttt ttacatgata aggttatcat
     3481 ttaaaaactc actttaatta ttcaacaata aaaaatcaac ttttttcaat aacttttgtg
     3541 gtttcttgtg taaaaccatt ataaaaattc tctctgtgaa attataattc tcctacataa
     3601 ttaatttttt gctcattata tcctacttta attttcttta aaaccttatt ataaaacatt
     3661 taaccttaat aataataaaa tttcttcctg attataacct tattacctta ttcaagcgat
     3721 tcattcatta caaaaaaaaa tttatctttt catatgacga aattctactt gttaatgttc
     3781 tttttgaaat gaataaccct gaggcaagcc gcgaggaatt cttttgatta aatacctttt
     3841 aatttgcatt taaacacttt tttaaaacga acataatctt ttgcttagaa gaagattttt
     3901 ctctatattg aaaaattttc tatatatatg caaacaattt ttacatttta tgtaattatt
     3961 attgaaatta acgaaatcaa aaagacagca aacgaggtgg ctttatcaag ataaccacct
     4021 cgtttgttta aaattatact tgaaaaataa acttattctt cttcttcgaa taccaattct
     4081 tcattttcca tatccagcac atttttacgg gcaaatgttt tcttttcgta ttcttcgcga
     4141 gaataaacta taattttctc gaaatcgcgt tgcccagtac ctgcaggtat taaatgtcca
     4201 cagattacat tttcttttag tccttcgagc ttatctactt ttccattgat tgcagcttct
     4261 gtcaaaactt ttgttgtttc ttggaaagaa gcagccgaca taaagctagt tgtttgaaga
     4321 gctgcacgcg taattccttg taaaatctga tttgaagtag cagggatggc atcgcgtgct
     4381 tgaaccagcc gtaaatcttt acgttttaat atactgtttt cgtcacgtaa ttttcgaggg
     4441 gttata
//
LOCUS       DAOTKS010000103         4409 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_17029_length_4409_cov_4.763895, whole genome shotgun sequence.
ACCESSION   DAOTKS010000103 DAOTKS010000000
VERSION     DAOTKS010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17029_length_4409_cov_4.763895"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="P5236_03095"
     CDS             <1..904
                     /locus_tag="P5236_03095"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HRU63381.1"
                     /translation="GAKSDTGNIGDIFHINNRVAVTDPSYPVYVDTNAMAGRAGVPNE
                     YNQWSNLVYLPCNEENNFVPALPKEKVDLIYLCYPNNPTGTTLTKEQLKVWVDYAREN
                     RAIILFDAAYEAFITENNVPHSIYEIEGATEVAIEFRSFSKTAGFTGTRCGYTIIPQQ
                     VMGYTRKGEAVSLNKLWLRRQSTKFNGTSYVVQRGAEATFTPDGRKQVKALIDYYLEN
                     ARIIREGLTKNGFAVYGGINSPYVWVKTPNGMSSWEYFDFLLKEKNIVTTPGAGFGIS
                     GEGYIRFSAFGSKEDTIEAMKRLA"
     gene            1031..2023
                     /locus_tag="P5236_03100"
     CDS             1031..2023
                     /locus_tag="P5236_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HRU63382.1"
                     /translation="MVSYKELGLVNSRELFKKAIEGGYAIPAFNFNNMEQLQAIISAC
                     VETKSPVILQVSSGARKYANQTLLRYMAQGAVEYAKELGYPIPIVLHLDHGDSFELCK
                     SCIEFGFSSVMIDGSHLPYEENVALTKKVVDYAHQFDVTVEGELGVLAGIEDDVKAEH
                     HTYTDPDQVVDFVSRTGVDSLAISIGTSHGAYKFKPEQCTVDANGRLVPPPLRFDILK
                     EIERRIPGFPIVLHGASSVPQEYVDEINRYGGQLKDSIGVPEEQLREAAKSAVCKINI
                     DSDGRLAMTAAIRRILWTQPAEFDPRKYLGPARDELKKLYMHKTQNVLGSAGKA"
     gene            2587..3078
                     /locus_tag="P5236_03105"
     CDS             2587..3078
                     /locus_tag="P5236_03105"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRU63383.1"
                     /translation="MNTLDLIILIPIVFGFIQGLFKGLIKELTSFAVIVIGIYGAKLL
                     TTPFEKLLLNIAFLSPQITKPLAFLLGFIIIAIGLFILAHLLDKTLSFIQLGGLNKFF
                     GGIVGGLKYLLILSILLNVFDTVDNHFHLINKTTKEQSLTYSKVLKVAPVLWEEVKDK
                     KID"
     gene            3132..3764
                     /locus_tag="P5236_03110"
     CDS             3132..3764
                     /locus_tag="P5236_03110"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019036106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRU63384.1"
                     /translation="MTPELKSRFSFHKYECGCDEAGRGCLAGPVVAAAVILPDDFVCP
                     QINDSKKLTSGVREKLRFIIEREALAWAVAEVSNVEIDQMNILNASILAMHKAIDKLS
                     IRPEFIIIDGNYFKPYADIPYQTIVKGDGKYLSIAAASILAKTHRDEIIEQLGEKFPQ
                     YYWQKNKAYPTFQHREAILKFGATPYHRMSYNLLGKKKHKKFDNFKKDNQ"
     gene            complement(3745..>4409)
                     /locus_tag="P5236_03115"
     CDS             complement(3745..>4409)
                     /locus_tag="P5236_03115"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRU63385.1"
                     /translation="NIETAGNFLYGLNHLLGSAPKNGSYQLFNIDYAQYVKTEYNITY
                     HQIFDSNNRFVYHAGIGVGAPYGNGKIIPYEKRFFSGGANSVRGWTESTLGPGVYKRI
                     NTLMRDYNQTGDVKLDLNMEYRAKLFWLLEGAAFIDAGNIWTIKDYETQPGGVFQWNN
                     FIDQIAIAYGIGLRFDLTIMIARLDLGIKLHDPVLKRSECWRLPPKWSDLAFHIAIGY
                     PF"
BASE COUNT         1377 a          724 c          820 g         1488 t
ORIGIN      
        1 tggagcaaaa agtgatacag gaaacattgg cgatattttt catattaaca atcgggtggc
       61 tgttaccgat ccaagctatc ctgtttacgt agataccaac gctatggcag gtcgagctgg
      121 tgtaccgaat gaatacaatc aatggagtaa tcttgtttat ttaccttgta atgaggagaa
      181 taatttcgtt cccgctttgc caaaagaaaa agtagattta atttatctgt gttatccaaa
      241 taatcctaca ggcacaactt taacgaaaga acaattgaaa gtttgggtag attatgccag
      301 ggaaaatcga gctattattc tttttgatgc agcctacgaa gctttcatta ctgaaaacaa
      361 tgttccacac agtatttatg agattgaagg agcaacagaa gtagcgatag aattcagaag
      421 tttttctaaa acggctggtt ttacaggtac acgttgcggt tataccatta ttccacagca
      481 agttatggga tatactcgaa aaggtgaagc agtgtcactt aataaattat ggctacgaag
      541 acaatcaacg aaattcaacg ggacttctta tgttgttcaa cgtggggcgg aagcaacgtt
      601 tactccagat ggaagaaaac aagttaaagc tcttattgat tattatttag aaaacgctcg
      661 aatcattcga gaaggattga ccaaaaatgg ttttgctgtt tatggaggta tcaattctcc
      721 atatgtttgg gtaaaaactc ccaatggaat gagcagttgg gaatattttg attttctgtt
      781 aaaagaaaaa aatattgtga ctactcccgg tgcaggtttc ggtatcagtg gagaaggtta
      841 tattcgtttt tctgcttttg gcagcaaaga agataccatc gaagctatga aaaggttagc
      901 ataataacct aaatgataaa gttttatctt ttattaaaat gttctttaac gctttctttt
      961 gtttttttct atttcacaga aaaatactac ttttgtaaaa taagattatt aatcaataaa
     1021 attcaagaaa atggtaagtt ataaagaatt aggtcttgta aattcaagag aattgtttaa
     1081 aaaagctatt gaaggaggct atgcaattcc agcatttaat ttcaataaca tggaacaatt
     1141 gcaagccatc atttctgctt gtgttgaaac aaaatcacca gtgattttac aagtatcaag
     1201 tggagcacgc aaatatgcca atcagacctt gttgcgttat atggctcaag gtgcagtaga
     1261 atatgcaaaa gaattgggtt atccaattcc tattgtgctt catcttgatc atggcgatag
     1321 ctttgaactg tgtaaaagtt gtattgaatt tggattttcg tctgtcatga ttgatggctc
     1381 tcatcttcct tacgaagaaa atgtagcatt gacaaaaaaa gttgttgatt atgctcatca
     1441 gtttgatgta acggtcgagg gtgaattagg tgttttagcg ggtatcgaag acgatgtgaa
     1501 agctgaacat catacttata ctgatccaga tcaagtagtt gattttgtaa gtcgtacagg
     1561 tgtagattct ttggcaattt ccattggaac ttctcatgga gcttataaat tcaaacctga
     1621 acaatgtaca gttgatgcaa acggtagatt agttccacca ccattgcgtt ttgatatcct
     1681 gaaagaaatt gaaagacgta ttccaggatt ccctattgtg cttcatggag cttcttcagt
     1741 accgcaagaa tatgtggacg aaattaatcg ttatggtggt caattaaaag attctatcgg
     1801 tgttcctgaa gaacaacttc gcgaagcagc caaatcggca gtatgtaaga ttaatattga
     1861 ttctgatggt cgtttagcta tgactgcagc tatccgaaga atattatgga cacaacctgc
     1921 agaattcgat ccacgtaaat atttgggccc tgctcgtgat gaattgaaaa aactttacat
     1981 gcataaaacc caaaacgttt tgggaagtgc tggcaaagca taattagctt aattataaga
     2041 atcccccgat gcttgcgtcg gggtagcttt tgtttttatt tttttagaaa ttccggtggc
     2101 gaagccataa aattggcgta atacctcgct gcttgcggcg aggatagtca attttaagga
     2161 atttctttat ttcaaaagaa ttcctcgagg cttgcctcgg ggttattcat tcttctgtaa
     2221 aacgtataca tttacgtttg ttcttctgcc tattttcgta tttgcgttgt gccaaatccg
     2281 ctctgtgtta cccgtttcga ttatcacagt ttcgacgcag ttcacacgat acaaccgatt
     2341 tatcttttcc gatagctaat gctatatctt tttgtttgca tttctgtgtt tccttgttta
     2401 aattatgtat ttttgtgcta tggttaatgt tttatttttg taatttttta tagggaaaaa
     2461 atcgaagtaa aacatttatt ccatcagggc aaaggggaaa aagttttttt cctttgccct
     2521 gaaaataatt ttttttcctt ttattccctc ttgaaaaagt tgcattaatt acttgaattt
     2581 acagtaatga atacactgga tttaattatt ttgattccta ttgtttttgg ttttattcaa
     2641 ggtttattca aaggattgat aaaagaatta acctcctttg ctgtcattgt cattggtatt
     2701 tatggagcaa aattattgac cactcccttt gaaaagttgt tgcttaatat tgcatttttg
     2761 tccccccaga taaccaaacc tttggctttt ttattgggtt ttattattat agctataggc
     2821 ttgtttatac ttgctcattt actggataaa acattatcgt tcattcaatt aggtgggttg
     2881 aacaaatttt ttggcggtat tgttggtggc ttaaaatatc ttcttattct cagtatcttg
     2941 ttgaatgttt ttgataccgt tgataatcat tttcatttaa ttaataaaac taccaaagaa
     3001 cagtcactga cttattctaa agttctgaaa gttgctcctg ttttgtggga agaagtgaaa
     3061 gacaaaaaaa tcgactgatc agaaaaatat ttattcaaaa aacaaaattg aatctaacaa
     3121 aaagaaataa aatcactccc gaattaaagt cccgtttttc atttcataag tatgaatgcg
     3181 gatgtgatga agcaggtcgt ggttgtttgg caggtccagt tgtagctgct gcagtaattt
     3241 tgcctgacga ttttgtttgt cctcagataa atgattctaa aaagttaact tccggtgtga
     3301 gagaaaagtt acgtttcatt atagaaaggg aagctttggc gtgggcagta gctgaagtaa
     3361 gtaatgtcga aattgatcag atgaatattc tcaatgcttc tattttggca atgcataaag
     3421 caatagataa actttcgatt cgacctgaat tcattatcat agatgggaat tattttaaac
     3481 cttatgctga tattccttat caaacaattg tgaaaggtga tggcaaatat ttgtctatag
     3541 ctgcagcttc catattagcc aaaacacacc gtgatgaaat tatagaacaa ttgggagaaa
     3601 aatttcctca atattattgg caaaaaaata aggcttatcc tacttttcaa catcgagaag
     3661 ccattttaaa atttggggca actccttatc atcgaatgtc gtataacctt ttaggaaaga
     3721 aaaaacacaa aaaatttgat aactttaaaa aggataacca atagcaatat gaaaggcgag
     3781 gtcgctccat tttggcggta gtctccaaca ttcactacgt ttaaggacgg gatcatgtag
     3841 ttttattcct aaatcaagac gtgctatcat aatagtaagg tcaaatctaa gtcctattcc
     3901 ataagcaatt gcaatttgat cgataaaatt gttccattga aaaaccccgc caggttgagt
     3961 ttcataatcc ttaatagtcc agatatttcc tgcatctata aaagcagctc cttcaagtag
     4021 ccagaataat tttgctctgt attccatatt caaatccagc ttaacatcac ccgtttggtt
     4081 atagtctctc atgagcgtat tgattctttt ataaacgcct ggtcccaaag tgctttcggt
     4141 ccatcctctt acgctatttg ctcccccgct aaaaaatcgt ttctcataag ggattatttt
     4201 tccatttcca taaggagcac ccacgccaat ccctgcgtga taaacgaatc tgttgtttga
     4261 atcgaaaatt tgatggtaag taatattgta ttcagttttt acatattgtg cgtaatcaat
     4321 attgaataat tgataagaac catttttagg agcacttccc aataaatgat ttaagccata
     4381 taaaaaattt ccagcagttt caatattgt
//
LOCUS       DAOTKS010000104         4399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_17076_length_4399_cov_4.216621, whole genome shotgun sequence.
ACCESSION   DAOTKS010000104 DAOTKS010000000
VERSION     DAOTKS010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17076_length_4399_cov_4.216621"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1646)
                     /locus_tag="P5236_03120"
     CDS             complement(<1..1646)
                     /locus_tag="P5236_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63386.1"
                     /translation="MRKTERYLCILLISVFFVGCNTTKFVPEGKYLLDKVDIKTDVRS
                     IKKNELYDYLRQTPNNSAFGLFRTQLGIYSLAGKDTTKWLNRFLMKIGEEPVIFNPTL
                     TDLSAQQLQLCFANKGYLNSKVDTKIEFYDKKARVEYIITSNQPYRIKNYNVQIPHNE
                     LAKIANDTLRSLIHRNMLFDIDVLDAERERITSRFRQMGYYNFRKDFLSYTADTTIAS
                     HQVDLTLHLNDNLLNPTDSLYSIIFKKFSIRNVTFYINATNISELQENGVTDTINFRN
                     FTMVTPSKQIIRIDALVQNTYINPKSVYNDVDVEKTYSALNSLGAIKYVNISFRETKD
                     SLLDCNIFIMPSSAISLTGEIEATYTDGYWGGAVNLNLINKNIFKGAETFSLQGHGAY
                     EWQKNIWAQEWGGQIGLKFPRFILPFASYDFKRNLHANTEFSGSYYYQFRPGEFRTTN
                     SQIGIKYLWNRSQYQHAINLIDINYVYFPEIFPTFREKFLDTGIFNRYNYENHFIMGI
                     GYSGSTTNYRSSSPLRNFFTMRYNIETAGNFLYGLNHLLG"
     gene            complement(1796..2878)
                     /gene="proB"
                     /locus_tag="P5236_03125"
     CDS             complement(1796..2878)
                     /gene="proB"
                     /locus_tag="P5236_03125"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HRU63387.1"
                     /translation="MELRFKKIVVKIGSNVLTQSDGTLDISIMSTLVDQIALLYKEGV
                     EIIIVSSGAVASGRSLLGINKKMDIVDQRQLYSTVGQAKLINLYWDLFQKHGITVGQV
                     LTTKQNFNSRRNYLNQRNCIKVMLENKIIPIINENDAVSVSELMFTDNDELSGLIASM
                     MDTEALFILSNIDGIYTGSLDDPFAKIIREIKKGEDISPYIQKTKSTFGRGGMQTKIN
                     IARKIADQGIEVFIANGRKENILIQLMDKNQEAICTHFVAEKSEVSSIKKWIAHSEDF
                     AKGEIYVNADAAKALKSDKAISLLPVGVTKIVGNFEKDDIVKIIDENGYSLGIGKIRY
                     DSTKARELIGKKHQKPLIHYDYMYLE"
     gene            complement(2888..3865)
                     /locus_tag="P5236_03130"
     CDS             complement(2888..3865)
                     /locus_tag="P5236_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007487476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRU63388.1"
                     /translation="MKKNLIIVLALIVFISCTASKKKNEKEAEKMNIPTFSSDSAYLY
                     IEHQTNFGPRVPNSSAHAACQQYLFHSLKRFGAEVIIQQADLQAFDGTILHAANIIGS
                     FSPAKENRLLLFAHWDSRPWADNDPEPANRRQPVLGADDGASGVGVLLEIARQLGKQL
                     PNIGVDIIFFDAEDYGNSQSFQNENTNNSWCLGSQYWAKHPHKQNYQARFGILLDMVG
                     ASQALFYKEYFSLTYAPQIVSKVWTEAKNLGFGQYFIDQQGGAITDDHFYVNNIRGIP
                     SIDIIHYDPNSATGFPPYWHTLHDTMENIDKNTLLAVGTTLLHIIYNEK"
BASE COUNT         1466 a          744 c          665 g         1524 t
ORIGIN      
        1 cttcccaata aatgatttaa gccatataaa aaatttccag cagtttcaat attgtaccgc
       61 atggtaaaaa aatttctcaa aggactagac gaacgataat tagttgtgga accagaataa
      121 ccgattccca taatgaagtg attttcgtaa ttataacgat tgaaaattcc tgtgtcaaga
      181 aatttttcac gaaaagtggg gaaaatttca ggaaaataga cataattaat atcaattaaa
      241 ttaatagcat gttgatattg agaacgattc caaagatatt taatgccaat ttgtgaattt
      301 gttgttctga attcacctgg tctgaattga taataatagg agccagaaaa ctcagtattg
      361 gcatggagat ttcttttaaa atcatagctg gcaaagggca atatgaaacg tggaaacttt
      421 agtcctatct gtcctcccca ttcctgtgcc catatatttt tttgccattc ataggctcca
      481 tgaccttgta atgaaaaagt ttcagctcct ttgaaaatat ttttgttgat taaattcaaa
      541 tttactgctc caccccagta accgtctgta taagtagctt caatttcgcc tgtaagagaa
      601 atagcagaag aaggcattat aaaaatgtta caatctaata aactatcttt cgtttcgcgg
      661 aaactaatat tgacatattt tatagctccg agtgaattaa gcgctgaata agttttttct
      721 acatccacat cattatacac acttttagga ttaatatagg tgttttgtac caaagcatca
      781 attctgataa tttgtttgct aggcgtaacc atagtaaaat ttctgaaatt gatggtgtct
      841 gtaactccat tttcttgtag ttccgaaata ttggtagcgt taatataaaa agttacattt
      901 cgtatactga atttcttaaa tataatacta taaagtgaat cggtgggatt gagtaaatta
      961 tcgtttaaat gaagagttaa atctacttga tgagaagcaa tagtagtatc agcagtataa
     1021 gaaaggaaat cttttcggaa attgtaataa cccatttgac gaaatcgtga ggtaattctt
     1081 tcgcgttcag catccagcac gtcaatatca aaaagcatat tgcgatgaat caagctccgc
     1141 aaagtatcgt tagcgatttt agctaattca ttatgaggaa tttgaacgtt ataattttta
     1201 atgcggtaag gttgattgga ggtaatgata tattctactc ttgctttttt gtcataaaat
     1261 tctattttcg tgtccacttt tgaatttaaa tatcccttat tggcaaagca aagttgtaac
     1321 tgctgagcag ataaatcggt aagagtagga ttaaaaataa caggttcttc acctattttc
     1381 attaaaaatc tattgagcca tttcgtagta tctttgcctg ctaaactgta aattcctaat
     1441 tgggtacgaa ataatccaaa ggcagaattg ttgggcgttt gtcggagata atcgtataac
     1501 tcattttttt taatagaacg aacgtctgtt tttatatcaa ctttatccaa taaatatttt
     1561 ccttccggaa caaattttgt cgtgttgcat cccacaaaaa atacactaat caacaaaatg
     1621 cataaatatc gttctgtttt tctcataaat gagatatttt gatttcttta gaaataattc
     1681 ctcatcaatt gctatcatta agctaattaa aaatagggaa agatatattt ttcaattctt
     1741 ttttataagt acaaaaaaga tggaataaaa tcataaacca caaaaacggt ttattttatt
     1801 ccagatacat ataatcataa tgaatcaatg gcttttgatg ttttttgcct attaattctc
     1861 ttgcttttgt actgtcgtaa cgaatttttc cgatcccaag tgaataaccg ttttcatcta
     1921 tgattttgac aatatcatcc ttttcaaaat tgccaacaat tttggtaaca cccacaggca
     1981 acaaacttat tgctttatca cttttcaaag cctttgcagc atcagcatta acataaattt
     2041 caccctttgc aaaatcctca ctgtgagcta tccatttttt tatactggaa acttcacttt
     2101 tctcagccac aaaatgtgtg caaatagcct cctgattttt atccattaac tgaatgagaa
     2161 tattttcttt tcttccatta gcaataaaaa cttctattcc ctgatcggca attttacgag
     2221 cgatattaat ttttgtttgc ataccgcctc ttccaaaagt agatttagtt ttttgaatgt
     2281 agggagaaat atcttctcct ttttttattt cacggataat ttttgcgaaa ggatcatcga
     2341 gcgaaccagt ataaattcca tctatgttgc ttaaaatgaa taaagcttcc gtatccatca
     2401 tcgaagctat aagtcctgaa agttcatcat tatcggtaaa cattagttca gaaacagaaa
     2461 cagcatcatt ttcattaatg ataggaatta ttttattttc gagcattacc ttaatacaat
     2521 tccgctgatt aaggtaattg cgtcgactat taaaattttg tttcgtagtt aatacttgac
     2581 caactgttat accatgtttt tgaaacaaat cccaataaag attgataagt tttgcctgtc
     2641 cgacagtaga atacaattga cgttggtcca caatatccat ttttttgttg atccctaata
     2701 aacttcttcc agaagctact gcaccagacg aaacaataat aatttcaaca ccttctttat
     2761 acaatagggc aatttgatca acaagggtag acataatgga aatatccaac gtaccatccg
     2821 attgagttaa aacattgctt ccaattttta ctactatttt tttaaaccga agttccatat
     2881 tgaattttta tttttcgttg tatatgatat gcaacaacgt tgttccgaca gccagtagag
     2941 tatttttgtc tatattttcc atagtgtcgt gtaaagtatg ccaataaggt ggaaaccctg
     3001 tagcactatt aggatcataa tgaataatat caatgctcgg tattcctctt atattattca
     3061 cataaaagtg gtcatcggta atagctccac cttgctgatc aataaaatat tgaccaaagc
     3121 caagattttt tgcttctgtc cacacttttg aaacaatttg tggagcataa gttaacgaaa
     3181 agtattcttt ataaaaaagg gcttgcgatg ctcctaccat atcgagcaaa ataccaaatc
     3241 gagcttgata attttgttta tgaggatgtt ttgcccagta ttgagaacca aggcaccacg
     3301 aattgtttgt attttcattt tgaaatgact gggaattacc ataatcttct gcatcgaaaa
     3361 agataatatc aactcctatg ttaggtaact gttttcccaa ttgacgagct atttctaata
     3421 aaacacccac tccgctggca ccatcatcag cacccaaaac aggttgtcga cgatttgctg
     3481 gttctggatc attatcagcc cacggtcgcg aatcccagtg agcaaaaagc aaaaggcgat
     3541 tttcttttgc tggagaaaaa gaaccaataa tatttgccgc atgaagaatg gtaccatcaa
     3601 aagcctgtaa atcggcttgt tgaattatta cttctgcccc aaatcttttt aaagaatgaa
     3661 atagatattg ttgacatgct gcatgagcag aagaattcgg aactcgagga ccaaaatttg
     3721 tttgatgttc tatatataaa taggctgaat cgctcgaaaa agtgggaata ttcatctttt
     3781 cggcttcctt ctcatttttc tttttagaag cagtacagga aataaaaacg attaatgcca
     3841 gaacaatgat caaatttttt ttcacttgat aagcaattta atttttttga aaagacagtt
     3901 gttaaaattc caatgaaaaa tcaatctaaa aatacaagtt acaaatttac atgatatttt
     3961 caatttaccc atgaaaaaga aaatcaactt ttcattaact caacagattt tcgggaataa
     4021 acgagattat gaaaggaaaa tttaaattaa gcaataactt tttaacaaaa atagaaaacc
     4081 ttttatcata cattagacat ttctaaattt tattattttt gtagataata ctaacttgct
     4141 ttctaacgga ttttcttttc tgaaaagtcg taatttattt ataataagtt ggatattatt
     4201 cacttacatg atcttcccta aaaaatttct tgcttacaaa ttatgtggta gctcaattgt
     4261 tcaatttatc taaaatgtat tgttgatctt catttaatcc caaaccctaa attttagggt
     4321 ttgagattaa aattgagtcc ttccaaatac ttcctttgag ttgatacaag tattgcacgt
     4381 actcctcttg cttatgatt
//
LOCUS       DAOTKS010000105         4394 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_17097_length_4394_cov_4.352155, whole genome shotgun sequence.
ACCESSION   DAOTKS010000105 DAOTKS010000000
VERSION     DAOTKS010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17097_length_4394_cov_4.352155"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2123)
                     /locus_tag="P5236_03135"
     CDS             complement(<1..2123)
                     /locus_tag="P5236_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HRU63389.1"
                     /translation="MMKGVNLILREFQDIYAQHPQIKALEKWTHSLEQNIKISGLSGS
                     SFALTLASLFKNVPQHFLIIQDDVDDAGYLYHDLKQLLSENEIFYYPSSYKQAIKLSQ
                     LDAANEILRTEVLNKLIHDATPCVVVTYPDAIMQKVIAPQYIQKQTLKLKVGGRANLD
                     EIAETLFQYGFNKVDFVYEPGQFSVRGGILDIFSFSSEQPYRCDFFGNEIESIRIFDI
                     ETQLSKGTKNEIEIIPNLHHDKVSPHVSFFEFIPKNTIIVINNPFFIKERINQLYDEI
                     LVKINAQKSIADLHQTHITGNELINFITPFRKIETGTEKNYFKITSRLNFNTLPQPVF
                     HKNFDLISDHLKKKIEEGYQVYILSDSTKQTDRIAMIFEDHGDNIPFIPVKNTLHEGF
                     IDEDLKILCYTDHQIFDRFHKYQLKTDQARHGKVIMTLKELNQLKIGDYVVHVDHGIG
                     TFGGLVRMENNGKMQEMVKLIYKDNDILFVSIHSLHRISKYKGKEGETPRINKLGSGA
                     WERLKEKTKSKVKNIARELIQLYAQRKAEQGFQFSLDSYLQQELEASFIYEDTPDQTK
                     ATADVKKDMETPIPMDRLICGDVGFGKTEIAIRAAFKAVADNKQVAVLVPTTVLALQH
                     YNTFKERLKDFPCNVEYLTRARSAKETKDILKRLSEGKIDIIIGTHKLVGKSVKFKDL
                     GLLIIDEEQKFGVAVKEKLKQMKVNV"
     gene            complement(2265..3815)
                     /locus_tag="P5236_03140"
     CDS             complement(2265..3815)
                     /locus_tag="P5236_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRU63390.1"
                     /translation="MLKKIRVVVSIVLFSLITLYFLDFRGFLPQSFSFLEKIQFIPAL
                     LAVNVAIIIILIALTLIFGRIYCSSVCPMGIFQDIIAWLSKKIIKRKRYTYSKAKTIL
                     RWSVLAAVVVTFLFGFTFLLGLLEPYGAYGRMATHLFRPIYMAGNNLLANILSSFGNY
                     SLYKVSIYSFGFFSTIIATITLLGIGFLAWRNGRTYCNTICPAGTLLGFLSNFSFLKI
                     QFKDERCNSCGLCVMKCKASCVDAKNKKIDYSRCVTCFNCIESCNRDAINYTFILQNT
                     KKNNPALTKDDNIKKIKSIDSSRRRFLSATLFSLVTGSTLLAQQTTSKLLPKKAFKRQ
                     TPVAPPGAISFEHFKKKCISCHLCVSKCPSHVIKPAFLEYGLSGVMQPHLYFDHGYCN
                     YNCTICGEVCPTGALKPLTVEEKHLTQMGQVQFIIENCIVYNDETSCGACSEVCPTQA
                     VSMIPYKDALTIPYIDTSICIGCGGCESACPAIPYKAIYVEGLKIQGKASIKHEQQEQ
                     IEIKDFGF"
     gene            complement(3836..>4394)
                     /locus_tag="P5236_03145"
     CDS             complement(3836..>4394)
                     /locus_tag="P5236_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRU63391.1"
                     /translation="TNSGIEAAAKAAGAKVVPANEESYYKPVSIPYGKNLKSTKIHEA
                     IVGSDKWINVPYLKNHGGAQLTISMKNYMGIVWDRGYFHAHDLQQCIADVCTYEKRPV
                     LNVVDAYRIMKNNGPRGKSPSDVVIAKGLFISQDIVAVDTAAANFFNQVREMPIEKVK
                     HILNAQALGLGTMNLDELKIKRIKL"
BASE COUNT         1451 a          767 c          709 g         1467 t
ORIGIN      
        1 acatttactt tcatttgttt taatttttct tttaccgcaa ctccaaattt ttgctcttca
       61 tcaatgataa gcaatcccaa atctttgaat tttaccgatt tacctactaa tttatgcgtt
      121 ccaatgataa tatcaatttt cccttcactt aatcgtttta gaatatcttt ggtttctttt
      181 gcacttcggg cacgggttaa atattcaaca ttgcaaggaa aatcttttaa tctttcttta
      241 aatgtgttgt aatgttgcaa agccaacaca gttgttggca ctagtaccgc aacctgttta
      301 ttatcagcca cagctttaaa agcagcacga atggcaattt ctgtttttcc aaaacctaca
      361 tcaccacaaa ttaaccgatc cattggaatg ggtgtttcca tgtctttttt tacatcagca
      421 gtagcttttg tctgatcagg tgtgtcttca taaataaacg aagcttccag ttcctgttgt
      481 aaatagctgt caagtgaaaa ttgaaaacct tgttctgctt ttcgttgtgc ataaagttga
      541 atcaattcac gagcaatatt ctttactttc gattttgtct tttccttcag tcgttcccag
      601 gctcctgaac caagtttatt aatacgagga gtttcacctt ctttaccttt gtattttgaa
      661 atgcggtgaa gtgaatgaat actcacaaaa agaatgtcat tgtctttata aatgagtttc
      721 accatttcct gcatttttcc attgttttcc atgcgaacca agcctccaaa agtgccaatc
      781 ccgtgatcca catgcactac gtaatcgcca attttcaatt gattcaattc tttgagcgtc
      841 ataatgactt tcccatgacg agcctggtcg gttttcaact ggtatttatg aaaacggtca
      901 aaaatctgat gatcagtgta acataaaatt tttaaatctt cgtcgataaa accttcatgc
      961 aaagtatttt tgacaggtat aaatggaata ttatcgccat gatcttcaaa aatcatggcg
     1021 attcggtcag tttgtttggt gctatcactg aggatataaa cttgataacc ttcttcaatt
     1081 tttttcttta aatgatcgct gattaaatcg aaatttttgt gaaaaacagg ttgtggaaga
     1141 gtattaaaat tcaatcgaga agttatttta aaataatttt tctctgttcc tgtttctatt
     1201 tttcgaaatg gcgtgataaa gtttattaat tcattccctg taatgtgcgt ttgatgtaaa
     1261 tcagcaatac ttttctgggc atttatttta acgagaattt catcatacaa ttgattgatt
     1321 cgctctttaa taaaaaaagg attgttgatg acaataatgg tatttttagg aataaattca
     1381 aaaaaagaaa catgaggcga aactttgtca tggtgcaaat ttggaataat ttctatctcg
     1441 ttttttgttc cttttgaaag ttgggtttca atatcgaata tacgaatgga ttcaatttcg
     1501 ttaccaaaaa aatcgcaacg ataaggttgt tcagaagaaa aagaaaaaat gtccagtatt
     1561 ccaccgcgaa cagaaaattg acccggttca tagacaaaat ctactttatt gaagccgtac
     1621 tggaatagtg tttctgcaat ttcatccaga ttagctcgtc caccaacttt taatttaagt
     1681 gtttgttttt gaatgtattg tggggcaatt actttttgca taatggcatc aggataagtt
     1741 accaccacgc aaggagttgc atcatgaatg agcttgttta atacttctgt tcttaaaatc
     1801 tcatttgctg catctaattg agataattta atagcttgtt tataagaaga aggatagtaa
     1861 aatatctcat tttcagaaag taattgtttc aggtcatgat aaagatagcc agcatcatcc
     1921 acatcgtcct gaataatgag aaagtgttga ggaacatttt taaaaaggga agctaaagtt
     1981 agagcaaaag aagaaccgct caaaccagaa atttttatat tctgttcgag ggaatgagtc
     2041 cacttttcaa gtgctttaat ttgtggatgc tgtgcgtaaa tgtcttgaaa ttctctaaga
     2101 atcaaattaa ctcctttcat cattacataa acatttaaca accaaatatt tatgtaatag
     2161 aaaattgttc taaaatttca ctgcaaaggt agtaatttta aaggaaagtg gaaaattaaa
     2221 aggatgagaa tttttaaaga aaacagttct gcaaggaaaa aaattcaaaa tccgaaatct
     2281 tttatttcta tttgttcttg ttgttcatgt tttatgctgg ctttaccctg aatttttaat
     2341 ccttctacat aaatagcttt gtaaggaatt gctggacaag ctgattcgca accgccgcaa
     2401 cctatacaaa tggaagtgtc tatataagga atagtaagtg catctttata aggaatcatg
     2461 ctaactgcct gcgtgggaca aacttctgaa caagcaccac aactggtttc gtcattatag
     2521 acaatacaat tttcaataat gaattgtact tgacccattt gcgtaagatg tttttcttct
     2581 actgttaatg gtttgagtgc acctgtagga caaacttcac cacaaatggt gcaattataa
     2641 ttgcagtaac cgtggtcaaa atataagtga ggttgcatta ctccacttaa accatattcc
     2701 agaaaagctg gcttaatcac gtgagaagga catttgctca cacacagatg acaggatata
     2761 catttttttt taaaatgttc aaaactaatg gcacctggag gagcaacagg agtttgacgc
     2821 ttgaatgctt tttttggaag taatttactg gtagtttgtt gagccagcaa tgtgcttcct
     2881 gtcacaagag aaaacaatgt ggctgaaaga aaacgtcgtc tcgaagaatc aatggatttt
     2941 atttttttta tgttatcatc tttagtaaga gctggattgt tctttttagt attttgcaaa
     3001 ataaaggtat aatttattgc atctcgatta caactttcga tacaattgaa acacgtaaca
     3061 caacgactat aatcaatttt tttattcttt gcatcaacac atgaagcttt gcacttcata
     3121 acacacaaac cacatgaatt acatctctca tctttaaatt gaatttttaa aaaagaaaaa
     3181 ttgctcaaaa aaccaagaag cgttcctgca ggacaaatag tattacaata agttctacca
     3241 tttcgccagg ctaaaaaacc aatccccaac aaagtaatgg tagcaataat agttgaaaaa
     3301 aatccaaaac tataaataga aactttatat aaactataat taccaaatga actcaaaata
     3361 ttagcaagta aattattccc agccatataa ataggacgga aaagatgagt ggccattctt
     3421 ccataagcac cataaggctc aagcaaaccc agcaaaaaag taaatccaaa aaggaaagta
     3481 acaacaactg cagccaacac actccagcga agaatagttt ttgctttgct ataagtgtaa
     3541 cgttttcttt ttatgatttt tttggataac caggctatga tatcttgaaa aattcccatt
     3601 ggacaaactg aagaacaata tattcgtcca aatattaatg tgagcgctat caaaattata
     3661 ataatagcca catttactgc taataaggca ggaataaatt gaattttttc taaaaaactg
     3721 aaactttgtg gcaaaaaacc tctgaaatcc agaaaataaa gagtaattaa tgaaaacaaa
     3781 acaatggaaa caaccactcg aatttttttc agcatacgtt tttattcagg aaaaattaca
     3841 acttaattct tttgattttt aattcgtcaa gattcattgt ccctaatccc aaagcttggg
     3901 cattaagaat atgttttact ttttcaatgg gcatttcacg tacctgatta aagaagttag
     3961 ccgctgccgt atccactgca acgatgtctt gtgagataaa aagtcctttt gcaatgacta
     4021 catcggaagg agattttcct ctcggtccat tatttttcat aattctgtat gcatcaacta
     4081 cgttcaaaac tggacgtttt tcgtaagtac aaacatcggc aatacattgc tgtaagtcgt
     4141 gagcatggaa ataacctctg tcccaaacaa tgcccatata atttttcata gaaatagtga
     4201 gttgtgcgcc gccgtgattt ttcaggtaag gaacattaat ccatttatca ctgcctacaa
     4261 tagcttcatg aatttttgtc gacttcaaat ttttaccata aggaatggaa acaggtttat
     4321 aataagattc ttcatttgct ggaacaactt ttgcgcccgc agcttttgca gctgcttcaa
     4381 taccactatt ggta
//
LOCUS       DAOTKS010000106         4366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_17214_length_4366_cov_4.073301, whole genome shotgun sequence.
ACCESSION   DAOTKS010000106 DAOTKS010000000
VERSION     DAOTKS010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4366
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17214_length_4366_cov_4.073301"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="P5236_03150"
     CDS             complement(<1..858)
                     /locus_tag="P5236_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HRU63392.1"
                     /translation="MKNDVLLKQVMEKAQRWLDGNYDEETKKEVRRMMSAEDKTELIE
                     AFYKDLEFGTGGLRGIMGAGSNRMNIYTVGAATQGLSNYLKLQFPEIPQISVVIGYDC
                     RNNSRRFAEVSANIFSANGIKVYLFDDLRPTPETSFAIRYLGCQSGIIITASHNPKEY
                     NGYKAYWSDGAQLVPPHDENVIAEVNKITNIDDIKFTGNPDLIQIIGEEIDKAFIEAV
                     KTISLSPEAIRRHKDLKIVYTPLHGTGIKLIPRALKEYGFENIIHISEQDVVSGDFPT
                     VISPNPEEPA"
     gene            complement(1406..2599)
                     /locus_tag="P5236_03155"
                     /pseudo
     CDS             complement(1406..2599)
                     /locus_tag="P5236_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785475.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            2969..>4366
                     /locus_tag="P5236_03160"
     CDS             2969..>4366
                     /locus_tag="P5236_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRU63393.1"
                     /translation="MIVNYSEQLHNVFQYSVQEAERLGNNYLGPEHLLLGILRNGKGK
                     AIDILNYSQINVSKIKQTIENQIRTDKEPDGEKIPVLKSTERIKKMMELEAKSLSAET
                     ADTEHLLLAILKEKDNLAGEILNEENLTYEIAKSYIEHSTDFQLNSSFQDDDDEEETF
                     HSQQKNISGSSKQSDTPALDTFGTDITKAAMENRLDPVVGRETEIERLAQILSRRKKN
                     NPVLIGDPGVGKSAIVEGLALRIVQHRVSRILFDKRIVALDMASIVAGTKYRGQFEER
                     IKAILNELAKNPNIILFIDEIHTIVGAGGTPGSLDAANMLKPALARGEIQCIGATTLD
                     EYRQSIEKDGALERRFQKIIVEPTTYDETLQILKNIQDRYEYHHNVRYTPEAIEACVR
                     LTDRYITDRCFPDKAIDALDEAGSRVHIGSISVPAEIEELEKRIEELKKQKMKVLSEQ
                     KYELAGPLRDQEKQYQ"
BASE COUNT         1561 a          777 c          705 g         1323 t
ORIGIN      
        1 agccggctct tctggattag gagaaataac tgttgggaaa tcaccactta ccacatcttg
       61 ttcagatata tgaattatgt tttcaaaacc atattctttt aaagcacgag gaattaattt
      121 aatgcctgtc ccatgaagag gagtataaac aatcttcaaa tctttatggc gacgaatagc
      181 ttcaggtgat aaggaaatag tttttacagc ttcaataaat gccttgtcaa tttcttcacc
      241 aataatttga attaagtcag gattaccggt aaattttatg tcgtcaatat tagtaatttt
      301 attcacttct gcaatcacat tttcatcatg aggaggaacc aattgtgctc catcgctcca
      361 ataagctttg taaccgttgt attcctttgg attgtgtgaa gctgtaataa tgatgccgct
      421 ctggcaaccc agatatcgaa tggcaaaaga agtttcgggt gtgggacgca aatcatcgaa
      481 caaataaact tttattccat tggccgaaaa aatattggcg gaaacttctg caaatcgccg
      541 actattgttt cgacaatcat aaccaatgac gacactaatt tgaggtattt caggaaattg
      601 cagcttcaaa taattgctca aaccttgtgt agcagctcct acagtgtaaa tattcatccg
      661 attactcccc gctcccataa ttcctcgcaa accacctgtc ccaaattcga gatctttata
      721 aaaagcttca atcaattctg ttttatcttc agcagacatc atccgacgta cttctttttt
      781 tgtttcttca tcataattac catccagcca cctttgagct ttttccatta cttgctttaa
      841 taaaacatca tttttcatat ttcatatttt ttaaattgtt atcaaaaatt aaagataata
      901 ctttttgctt tcagatacaa aaactgtgtt atttcttaat atttggcaat tccaactcat
      961 aacctttttt tcggtcttca tcttttaaca taaatccaac cacaagtgcc aatataccaa
     1021 aaatcataaa taccaaactt gcataagtat aattataacg taacgcaatt ttatgctgaa
     1081 cgatttcctt agaatctatt tttacacaaa ttttttcaac aatttcttcc ttttcttttt
     1141 tattttcagg caattctatt tgttgaagag ctttcagatt ttcttcaact attttgtttt
     1201 taaagaaagc aaagcctgcg gtatcattaa ctgcttcata attagtgttt attaaaatat
     1261 tattaattaa attagtagta gttttatcca cagctttttc caattcaatt ttatttaatt
     1321 gcaataccgg ttgtaattgc aaataagttt cactaatact attttttatc atttcttttg
     1381 cttttgaaac ggtaggattg gtattgttca acactacgcc aataagcaat ggaaccagca
     1441 tcaatcccca gttttgtatc caaaatatca aagcataagc tgtcccaagt tgacgttcag
     1501 gaataatttt tgggacagaa ggccacattg ccgatggaac caatgataaa gctattccca
     1561 aaataattgt cacaataata gccaatagcc agtgattgaa aaaaggaata gagaaaaaac
     1621 catgtacaat aattaataaa gcagcaccaa taatcataat ggtagctcct tttccttttc
     1681 ggtcataaat atttccaaat aaaggggtta aaaaaagagt acccagtggt aggacgctgg
     1741 gaattaatcc tgccaaattc ggatcaacac ggtatttatt gattactaaa tcagttgcaa
     1801 acttaatcca tgggaaaaca gcggaataaa acaacacaca aagaatagct ataagccaga
     1861 aacccttatt tttaatgata gtaaatacat ctgaaacttt aaaaggttct tcttcctctt
     1921 ttggcaaatc agtttctgaa gcttccaact ttttatccat ctgaacataa actatataag
     1981 caatcaagcc aatacaaagc aaaagaactc caaaaagaat aggtacagat acatcattaa
     2041 aatgtcttgc aataggaaga ggaattgcca ttgccagcaa ggttcccaga cgagccgttg
     2101 ccatttgaag tcccatagcc agagccattt ctttcccttt aaaccatttt acgatcacac
     2161 gtgaaacagt gatgcccgca atttcaactc cgacggcaaa tgttgcatag cctaatgctg
     2221 caattattac ttgagctttt ataccaaaaa tcggagaaag accttccggg aaaaaaggtg
     2281 atatagcaaa atacttaatt gaagcaccta tcaccattac tgaagtcgca gccaaccctg
     2341 tgaacttcac tcccatttta tctaggataa tgccaccaaa aatcaacatc aataggaaaa
     2401 cattgaacca accgtaggca ctggtaaaaa tggaataatc aatattggtc cacagcaact
     2461 gactttcaag taaaggtttt agaggactca ttgcatcggc tataaagtaa ccgcataaca
     2521 tggtaaacga tacaatgcct aaagccgtcc atctaactaa ggaagattct cgaagcgaaa
     2581 cagcaaactt ttctgacata aaattgtttt taattaggaa aattctaaaa gaaaaagaga
     2641 atatttacgt ttttagggtt acaaaactaa tgaaaaaaat ttaattgttt ttttactttt
     2701 attcaataac taactttctt attatgataa tctttaaaat tttttatttt tgacaatatt
     2761 tcaccttgaa gtgacaaaat tacagtattt tactataaat gcctacggta ttatttttga
     2821 tagaaaataa gaaatttaat tcttgattct gagtaaacaa aaaagaaaat aaaattaaaa
     2881 atcaaaagtt aaaaaccaaa aaacatacaa gaaacataca aactcaacat tatttcaatt
     2941 atatttgtag caaaggagaa aatcaatcat gatagttaat tattctgaac aattacataa
     3001 tgtatttcaa tacagcgtcc aagaagcgga acgattagga aataattatc ttgggcctga
     3061 acacttgtta ttgggaattc ttcgcaatgg aaaaggtaaa gccatcgata tactaaatta
     3121 ttcacaaata aacgtatcga aaattaaaca aaccattgag aatcaaatac gtactgataa
     3181 agaacctgat ggtgaaaaaa ttcctgtttt aaaaagtaca gaacgtataa aaaaaatgat
     3241 ggaattggaa gccaaatctt tgtcagccga aactgctgat acagagcatt tgcttttagc
     3301 tattttaaaa gaaaaagata atctggcagg tgaaatatta aacgaagaaa atcttactta
     3361 tgaaatagca aagtcatata ttgaacattc tactgacttt cagttaaatt caagtttcca
     3421 agatgatgac gatgaggaag agactttcca tagccagcaa aaaaatattt ctggtagctc
     3481 aaaacaaagt gatacaccag cattggatac ttttggaaca gatatcacaa aagctgctat
     3541 ggaaaatcgg ttagatccag tggtgggaag agaaactgaa atcgaacgtc tggctcaaat
     3601 tttaagcaga agaaagaaaa ataatcctgt tttgattggc gatccaggag ttggtaaatc
     3661 agccatagtt gaaggattgg ctttacgaat tgttcaacat cgtgtttcgc gaattttgtt
     3721 tgataagcga attgtagctc ttgatatggc ttctattgta gcaggaacaa aatacagagg
     3781 acaatttgaa gaacggataa aagccatttt aaatgaactg gcaaaaaatc ctaatatcat
     3841 tctctttatt gatgaaattc ataccattgt tggtgcgggt ggaacgccag gatcattaga
     3901 tgctgcaaac atgctcaaac cagcattggc gcggggtgaa atacaatgta tcggtgctac
     3961 tacgttggat gaatatcggc aaagtattga aaaagatggt gcgttagaac gtcgttttca
     4021 aaaaattata gtagaaccaa ctacttatga tgaaacttta cagatattga aaaacattca
     4081 agataggtat gaatatcatc ataacgttcg ttacacgccc gaagcaatcg aagcttgtgt
     4141 aagactgact gatcgttata taacagatcg ctgtttcccc gataaggcaa ttgatgcttt
     4201 ggacgaagca ggttcgagag ttcatattgg ttctatttct gttcccgcag aaatagaaga
     4261 actggaaaaa cgaatcgaag agctcaagaa gcaaaaaatg aaagtgcttt ccgaacaaaa
     4321 atatgaattg gccggtcccc tccgcgatca ggaaaaacaa taccaa
//
LOCUS       DAOTKS010000107         4358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_17248_length_4358_cov_6.819661, whole genome shotgun sequence.
ACCESSION   DAOTKS010000107 DAOTKS010000000
VERSION     DAOTKS010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17248_length_4358_cov_6.819661"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1640
                     /locus_tag="P5236_03165"
     CDS             <1..1640
                     /locus_tag="P5236_03165"
                     /inference="COORDINATES: protein motif:HMM:NF025943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chondroitinase-B domain-containing protein"
                     /protein_id="HRU63394.1"
                     /translation="KPILIKSKNLFGAKITGASYFNPQGQSYVTYEGFKFELSPVSTI
                     FKMEGCSYVRITRNWFTMNSDATKNSKWITVGEIWANETCRSHHNRIDHNLFEGKHDM
                     GALLVIDGSHGTVPAISKYDRIDHNIFRDNTPRVDNEKETVRIGVSDLSNLDAYTVVE
                     NNLFENCDGDPEIVSVKSYSDTIRNNTFKGCLGTICLRQGKGSVVEGNFMFGENKTVL
                     FNGEMIGCGGVRMYGLNHKIFNNYFEGLTGDKWDAALTITNGDVTNSSTSLSSHFLPE
                     NIIITNNTLINNVSNIEIGFDNSGSYGKPPKNCVIANNIITAAANPLVKYYSTTSLTG
                     VNFVNNIFYPTETATIGLTGFNETQIKNIDPQIILTDCRAYDQNCNNKLPFLLYKLTS
                     SSPAVNASTPYDFVTTDFERQPAVGIRDIGADENNAVSPVVNGPMDETKAGPEAPENY
                     TYELNAIAGVENIVNNGILIMPNPFNGTTQLTIHNTKAGKITVNLYNTLGEQIQQKEI
                     ETNGGMFEMQISTKQKGLLFCLIRLSNKTYSLKLISK"
     gene            1681..3789
                     /locus_tag="P5236_03170"
     CDS             1681..3789
                     /locus_tag="P5236_03170"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRU63395.1"
                     /translation="MKKITLLLILCILTDLTMKGQLLYSDDFSTYSVGNLEGQNGWVV
                     SLKSADANGSSPLVVDEQISYSNYIHSGQGKVVRYVTEGVDGSNSRISAKSWGNAITS
                     GSLYVGFMVRFVNATQSNTSGGRDFFGATFNNSGGTTLRGRIFLRKGTAANTIQLCIT
                     KNSTPSGTGTYWSGDLSINDTHFLVVKYTIVGTANSKESDDTYRLYIDPIIGSNEASA
                     TIFTDVFDATTVADGSGLNNFCLREKGDDMKISGIRVATTWEDVVKASPSSLIQLSAP
                     TVGTPTNVTDNGFTAHWTPVDNATGYDVKVYLGANLINTINVSGQSTSSVEITGLMSG
                     MTYTYQVVAKGDGTTYGDSDPSASSSEITTLDPYATDAIYTDFNDGSWGEIASSTPSS
                     GSYPSYSVNGFDMTAATLCVGSMRGIKGESHTNRIAIDKLAYGGKVALPTVNALKQIE
                     IHATAGTAGNGFQLKEFNPSTNSWSTIGSTYVYDANSKAAGTDSIYIISLDRISPTKL
                     RIDNPTNGAIYIWQIITRNTNPALLAKPVVSYASNVTSSGATANWTPVENATGYKIYV
                     YQGSTLIEGAPFSVSGQTINSFDIMNLATSTTYSYKVQAIGDGDINYSDSFLSSAIEF
                     TTLVTGIDNLKNNPNITVVNKKIICPETGDMRIFNMQGGLIESVKNVKTVQTDLPSGI
                     YIVKFSNQNGETKIQKIAIQ"
     gene            4142..>4358
                     /locus_tag="P5236_03175"
     CDS             4142..>4358
                     /locus_tag="P5236_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63396.1"
                     /translation="MKKLILLFSVGFAFFMAKAQEPIFTEYFETAPIGGDLEGYNDWH
                     VSPKSSEANGVSPKIAEGALFYTGYAGS"
BASE COUNT         1487 a          718 c          800 g         1353 t
ORIGIN      
        1 taaaaccaat tttaataaaa tcgaaaaatc ttttcggagc aaaaattact ggtgcatctt
       61 atttcaatcc acagggacaa agctatgtaa cttacgaagg ttttaaattt gaactcagtc
      121 cggtaagcac cattttcaaa atggaaggtt gcagttatgt gcgtattacc agaaactggt
      181 ttaccatgaa ttctgatgcc acaaaaaatt cgaaatggat aacagtagga gaaatttggg
      241 caaatgaaac atgtaggagc catcataaca gaatcgatca taatcttttt gaaggaaaac
      301 atgatatggg ggcattactc gttattgatg gaagtcatgg aaccgtaccg gcgatttcaa
      361 aatacgaccg tatcgatcat aatattttca gggacaatac tcctcgtgtg gataatgaaa
      421 aagaaactgt ccgcattggc gtaagtgatt tgagcaatct cgatgcttat acagtagtcg
      481 aaaataattt attcgaaaat tgtgacggcg atccagaaat cgtttcggta aaaagttaca
      541 gcgataccat tcgaaataat actttcaaag gttgtcttgg aaccatttgc ctgcgacaag
      601 gtaaagggag tgtagtggaa ggtaatttca tgtttggaga aaataaaacc gtacttttca
      661 atggagaaat gattggatgt ggtggcgtaa gaatgtatgg attaaatcac aaaattttca
      721 acaattattt tgaaggactt accggtgaca aatgggatgc tgccttaaca atcactaatg
      781 gagatgtaac caattcatct accagcctgt ccagccattt tttacctgaa aatattatca
      841 ttaccaacaa tacgttaatc aacaatgtat cgaatataga aattggtttt gataatagtg
      901 gttcatatgg aaaaccaccg aaaaattgtg tgattgccaa caatattatt acagccgcag
      961 ccaatcctct ggtgaaatat tatagtacca cttccctgac aggcgtcaat ttcgtaaaca
     1021 atatctttta tccgactgaa actgccacca ttggattaac aggttttaat gaaacacaaa
     1081 ttaaaaacat tgatccacaa atcattttaa ccgattgcag agcttacgat caaaattgta
     1141 ataataaact tccttttttg ctttataagt tgacttcttc aagtccagca gtgaatgctt
     1201 ccacacctta cgattttgtt acaaccgatt ttgaaagaca accagcagtt ggaataagag
     1261 acatcggagc agacgaaaac aatgctgttt ccccggtcgt taacggaccg atggatgaaa
     1321 caaaagccgg accagaagcg cctgaaaatt acacctatga gctaaatgca attgcagggg
     1381 tagagaatat tgtaaacaat ggcattttaa tcatgccaaa tcctttcaat ggtactacac
     1441 aattgacaat tcacaatacc aaagctggga aaataactgt aaatctatac aatactctgg
     1501 gtgaacaaat tcaacaaaaa gaaatagaaa caaatggtgg catgttcgaa atgcaaatct
     1561 cgacaaaaca aaaaggttta ttattctgcc ttattcgctt atccaataaa acatatagtc
     1621 taaaacttat ttctaaataa aaattcactt aacaaattca caatttaaaa ttttacaatt
     1681 atgaaaaaaa ttacattact attaattctg tgtattttga cagatttgac aatgaaaggt
     1741 caattgttat attcagatga tttttcaact tattctgtag gtaatctcga aggtcaaaat
     1801 ggttgggttg tatctttaaa gtctgctgat gctaatggtt cttctccgct ggttgtggat
     1861 gagcaaattt cttattcaaa ttatattcat tcagggcaag gaaaggttgt tcgatatgtt
     1921 acagaaggag tggatggttc aaatagtcga atttctgcaa aaagttgggg aaacgctata
     1981 acttctggct cattatatgt gggttttatg gtaaggtttg taaatgcgac tcagagtaac
     2041 acaagtggag gaagagattt ctttggagca acgtttaata attcaggagg aaccacttta
     2101 agaggtcgaa tttttttacg gaaaggcaca gcagctaata caatccagct ttgtattaca
     2161 aaaaattcaa caccatctgg gactggaact tattggtcag gagatctttc tataaatgat
     2221 acccactttt tagtagtcaa atatacaatt gtcggaactg caaattctaa agaatcagat
     2281 gatacttacc gactatatat agatccgata attggtagta atgaagcaag tgcaaccatt
     2341 tttacagatg tatttgatgc aactactgtc gccgatggat caggcttaaa taatttttgt
     2401 ttgagagaaa aaggtgatga tatgaagatt tcaggtataa gagtcgcaac aacatgggaa
     2461 gatgtagtaa aagcatctcc atcaagttta attcaattaa gtgccccaac cgttggtact
     2521 ccaactaatg tgacagataa tggatttact gctcattgga caccggtaga taatgcaacc
     2581 ggttacgatg tgaaagttta tttgggagct aatttaatta acactattaa tgttagcggg
     2641 caatctactt caagtgttga aataacgggc ttaatgtcag gaatgactta cacttatcaa
     2701 gtggtagcca aaggagacgg tacaacttat ggcgattctg acccttcagc ttcttcttct
     2761 gaaattacaa ctctcgatcc ttatgctacc gatgctattt ataccgattt caatgatggt
     2821 tcatggggag aaattgcaag cagtacgccg tcttcaggtt cttatccgag ttattcggtc
     2881 aatggctttg atatgacagc tgccacattg tgtgttggtt caatgagagg aatcaaagga
     2941 gaatcgcata ccaaccgtat tgctattgac aaattagcgt atggagggaa agtagcattg
     3001 cctactgtca atgctttgaa acaaattgaa attcatgcta cagcaggaac agcaggaaac
     3061 ggctttcaat taaaagaatt caatccaagt accaattctt ggtcaaccat tggctctact
     3121 tacgtttatg atgctaattc aaaggctgca ggcactgatt ccatttatat tatttcattg
     3181 gatagaattt ctcctaccaa gctcaggatt gacaatccta ccaatggagc aatttatatt
     3241 tggcaaatta ttacccgcaa taccaatccc gctttattgg ctaaaccggt tgtttcgtat
     3301 gcatccaatg ttacctcatc aggagctaca gctaattgga ctccagtaga aaatgcgaca
     3361 ggctataaaa tttatgttta tcaaggatca acgttaattg aaggagcacc tttcagtgtt
     3421 agtggacaaa ccattaacag ttttgatata atgaatttgg ctacatccac aacatattct
     3481 tacaaggttc aagccatcgg agatggggat ataaactatt ccgattcatt tctttcttca
     3541 gctattgaat ttacaacgct ggttactgga atagataatt taaaaaacaa tccgaatatt
     3601 accgtagtaa ataaaaaaat aatttgtccc gaaacaggtg atatgcgtat tttcaatatg
     3661 caaggtggct tgatagaatc ggtaaagaat gtcaaaactg ttcaaactga tctcccttca
     3721 ggaatttata ttgttaaatt cagtaatcaa aatggtgaaa ccaaaattca aaaaatcgcc
     3781 attcagtagt ttattcagca agaatcatat cggcattttt tcgtgcagat atgattcttt
     3841 aaaatgttgc tggaagaaaa acctaaattc aagtaattaa tacaactttt ttaagaaaat
     3901 aataagtaga aaaaagagag aaaatttaat aaaaagttga gattaataaa ttctttttaa
     3961 tttttaaaag attaaaatca ttgaataaaa gttcttgtat taatttcatt tgtctttcaa
     4021 aattattcta ataaaatttg ttttttatta aaatatacct tatttttggt cgaccaattt
     4081 tttaaaatga gaattttttg taataatttt tttaatgtct aacttttaat ttctttacat
     4141 tatgaaaaag ctaattttac ttttttcagt cggttttgct ttttttatgg caaaagcaca
     4201 agagccaatt tttacggagt attttgaaac agcgcccatt ggtggcgact tagaaggtta
     4261 taatgattgg catgtttctc ctaaatcttc agaagcaaat ggagtgagtc ccaaaattgc
     4321 tgagggggct ttgttttaca ccggttatgc aggttcta
//
LOCUS       DAOTKS010000108         4349 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_17299_length_4349_cov_4.600140, whole genome shotgun sequence.
ACCESSION   DAOTKS010000108 DAOTKS010000000
VERSION     DAOTKS010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17299_length_4349_cov_4.600140"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="P5236_03180"
     CDS             <1..275
                     /locus_tag="P5236_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005789131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRU63397.1"
                     /translation="ILDEFIFRIEAVQMAEGKKYVLMNVPTDNLNKIIEIIPGIKSPT
                     IMPLAKEGWHSLHAVISEKEFWDIIEKLKACGAEGILVLPIEKIIP"
     gene            321..1049
                     /locus_tag="P5236_03185"
     CDS             321..1049
                     /locus_tag="P5236_03185"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRU63398.1"
                     /translation="MNLCIDQGNSSTKAGIFDKDKLIQCFSYQNFNIKILLSIFSDFP
                     IENCIFSTVIEKDPIFINFLREKIPFVIVLDDKTPIPIINKYLTPESLGNDRLAGVVG
                     ASFLKPNSDVLVVDAGTAITYDFIDANKTYCGGNISPGIDLRIKSLHEFTQKLPLVEP
                     TPNIPILGVDTYTAIAAGVIQGIAFEIDGYIDFLRLQYPKLLTFLTGGSVFYFDKKLK
                     NTIFVEKYLVLIGLNHILQYNVKP"
     gene            1081..2289
                     /locus_tag="P5236_03190"
     CDS             1081..2289
                     /locus_tag="P5236_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63399.1"
                     /translation="MIQLSYSQNNTNSPYTRFGYGEINDNHNGEQRAMGGVAMGVRSS
                     ASINSVNPASYSVVDSMTFMFDMGASLLVSNFSSGNNKKTTFTGNLDYLTIQFPLAKK
                     IGVSAGIQPYSFSGYNFSETQLLFLANNTDTPDSLTATKSFVGSGGFSEFYAGLSFEL
                     FHHLALGVNAYYMFGSINNYRNISFNQSTNFTSTTQLNYIEANNFRFRYGGQFFTTFA
                     KKHEITLGLIYEQKKNFNGNASTIISGISVDTINYDLAFDLPTIFGAGLYYTYHNKLS
                     VGLDYSFQNWEEARFYGKTDTLANRHKIAIGGEYIPNPNGRKYFEHIRYRVGFNTSNP
                     YYKIEGISPGKNFGINFGVGLPLRYSRTIINASFEYGKIGNYSKLKENYFKFTFNACI
                     NENWFFKRKL"
     gene            2316..3713
                     /locus_tag="P5236_03195"
     CDS             2316..3713
                     /locus_tag="P5236_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63400.1"
                     /translation="MKTKITILFLMGLILLSPKGFAQKDKKDKTKATDQEFTSTQNPS
                     QQVTDSMCLIYISLFNESAKNKQYADAYGPWLSAYNECPGANKVIYTRGREILQWKLG
                     QAKDQTSYKETFDELMRMYDKRIQYFGDDSRYPTPWILGVKALDYITYVKGDDLKKPA
                     YEWLEQSIDGMKENSELEVLRQFIMLSYNIYKAEPTHAEKFIADYLKVNEILEAQINN
                     PEFKYAELASQIKQGLDVMFAQSGAADCKTLDEIYKSKVSQNISNLEYLNNVLDFYSR
                     IGCFDSQVYFQAAIAAHKIQPTATSANACAEMSYKKKNFNQAIQFYEEATNLATNNNE
                     KADYLYKIAQIYYSELQNYLKARDYARKSLEYNPNNGSAYLLIGIMYANTKGIYDDPV
                     LAKTVFWVAVDKFIKAKQVDPDPKIKEEADKLIQTYSRYFPSKEDIFFHPELEEGKSF
                     YVGGWIGETTICRPY"
     gene            3785..>4349
                     /gene="lptC"
                     /locus_tag="P5236_03200"
     CDS             3785..>4349
                     /gene="lptC"
                     /locus_tag="P5236_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HRU63401.1"
                     /translation="MFFFMIGCNKEKPEIIQAITDRAATPNLHATDVTTIISDSGITR
                     YRISAPQWDIYDKVPEPYWEFPYGIHLERFDMNLHVDANIHSKYAKYYINQKLWELKG
                     NVKATNLKGELFETEQLFWDQNSERIYSDSLIKVTRPTQIINAIGFESDQSMNYYVFH
                     KVKGIFPYEESQDSFPPNPQQTEISPSK"
BASE COUNT         1560 a          672 c          704 g         1413 t
ORIGIN      
        1 aaattctgga tgaatttatt ttcagaattg aagccgtaca aatggcagaa gggaaaaaat
       61 atgttttgat gaatgttcca acagataatt taaataaaat cattgaaatt attcctggca
      121 ttaaaagtcc caccatcatg ccattagcaa aagaaggatg gcattctttg catgccgtaa
      181 tatctgaaaa agaattctgg gatattattg aaaaattgaa agcttgtggt gctgaaggca
      241 ttttagtgct tccaattgaa aaaataatcc cttaaattca atattcatgg ctgatataat
      301 ttaatctatt tcaatttttt atgaatttat gcattgatca aggaaattca agtaccaaag
      361 caggaatatt tgataaggat aaactaatcc aatgcttttc gtatcaaaat ttcaatataa
      421 agattctttt atcaattttt tcagattttc ccatagaaaa ttgtatattt tcaactgtta
      481 ttgaaaaaga tcctatattt atcaattttt taagagagaa aatacctttt gtaattgttt
      541 tagatgataa aactcctatc cctattatca ataaatattt aacccccgaa tccttaggaa
      601 atgatcgttt agctggagtg gtaggtgctt catttttaaa gccaaactca gatgtactgg
      661 ttgttgatgc aggaacagca ataacttacg attttattga tgccaataaa acttattgcg
      721 ggggtaatat ctctccaggc attgatttaa gaattaaatc tctccatgaa ttcactcaaa
      781 aattaccact tgttgaacca acacccaata ttccaattct tggagttgat acgtatactg
      841 caattgcagc gggtgtaata caaggtattg ctttcgagat agatggctat atagattttt
      901 tacggttaca atacccaaag cttttaactt ttttaacagg aggtagtgtt ttttactttg
      961 ataaaaagct aaaaaacacc atctttgtag aaaaatattt agtactaata ggccttaacc
     1021 acatacttca atataatgtt aaaccataag gtattatttt ttatctttcc tactttaatg
     1081 atgatacaac tatcttatag tcaaaacaat acaaattctc cttacactcg ttttggatat
     1141 ggagaaatca atgataacca caatggtgaa caacgtgcta tgggaggagt tgctatggga
     1201 gttaggtcat cagcaagtat taattctgta aatcctgctt cttatagcgt agtggatagt
     1261 atgacattta tgtttgatat gggagcttct ttgttggttt ctaatttttc aagtggtaat
     1321 aacaaaaaga ctacttttac aggaaatttg gattatctta ctattcagtt tcctttggca
     1381 aaaaaaatcg gcgtcagtgc tggaattcaa ccatactctt tttctggata taatttcagc
     1441 gaaactcaac tcctcttttt agccaataat actgatacac ctgatagcct cactgctact
     1501 aaaagctttg ttggttcagg gggattcagt gaattttatg ctggtttatc cttcgaatta
     1561 tttcaccatt tagcattagg cgtaaatgcc tattacatgt ttggaagtat caacaattat
     1621 agaaatatca gttttaatca atcaacaaat tttacttcca ctacgcaact aaactatatt
     1681 gaagcgaata atttcagatt cagatatggg ggtcagtttt tcacaacttt tgccaaaaaa
     1741 catgaaatta cattagggtt aatttatgaa caaaagaaaa atttcaatgg aaatgcttca
     1801 accattattt ctggcatttc tgtggacacc ataaactatg atctagcttt tgatttgcca
     1861 acgattttcg gagctggttt atattatact taccataata aactttcagt aggacttgat
     1921 tatagttttc aaaattggga agaagccaga ttttatggga aaacagatac cttagctaac
     1981 cgccataaaa tagctatagg aggagaatat attccaaacc ctaatggaag aaaatatttt
     2041 gaacatattc gttaccgtgt tggctttaac acaagtaatc cctattataa aatagaagga
     2101 atttctccag gaaaaaattt tggtataaat tttggagttg gtttaccatt acgatacagt
     2161 agaacaataa tcaatgcttc tttcgaatac ggtaaaattg gaaattacag caaactaaaa
     2221 gaaaattatt ttaagtttac tttcaatgcc tgtatcaatg aaaattggtt ttttaaacga
     2281 aaattataaa acaactttta aaaatatttt ttattatgaa aactaaaata acaattttgt
     2341 tcttgatggg tttgatcctt ctcagtccta aaggatttgc tcaaaaagat aagaaagata
     2401 aaacaaaagc tacggatcag gaattcacaa gtactcaaaa cccttcacag caggtaactg
     2461 attcgatgtg tttgatttat atcagtttat tcaatgaatc agctaaaaat aaacaatatg
     2521 ctgatgctta tggtccctgg ttatcagctt acaatgaatg tccaggagca aataaagtaa
     2581 tatacaccag aggtagagaa attctccagt ggaagttagg acaggcaaaa gatcaaacct
     2641 cctacaaaga aacctttgat gaattaatga gaatgtatga caaacgcatt caatatttcg
     2701 gagatgacag tcgctatcca actccttgga ttttaggagt aaaagcctta gactacatca
     2761 cttatgtaaa aggagatgat ttaaaaaaac ctgcttatga atggttagag caatccattg
     2821 atgggatgaa agaaaattct gaattagaag ttcttagaca atttataatg ttgtcttaca
     2881 atatttacaa agcagaacct acacatgcag aaaaatttat tgctgattat ttaaaagtta
     2941 atgaaattct tgaagctcaa attaataatc ccgaatttaa atatgctgaa ttagccagtc
     3001 aaataaagca aggattagat gtaatgtttg cccaaagtgg tgctgctgat tgtaaaactt
     3061 tagatgaaat ttataaatcg aaagtatctc aaaacatttc aaatttagag tacttgaata
     3121 atgtgttaga tttttatagt cgaattggtt gttttgactc tcaggtttat ttccaagcag
     3181 ccattgctgc ccataaaata caaccaactg ctacatcggc taatgcatgt gctgaaatgt
     3241 catacaaaaa aaagaatttc aatcaagcaa ttcaatttta tgaagaagct actaatttag
     3301 caacaaataa caatgaaaag gctgattatt tatataagat agctcaaatt tattattcag
     3361 aattgcaaaa ttatctgaaa gcaagagatt atgctcgtaa atcattagaa tacaatccca
     3421 acaatggcag tgcttatctg ttgattggaa taatgtatgc aaatacaaaa ggaatttatg
     3481 atgatccggt tttagctaaa actgtttttt gggtagcagt agataagttt ataaaagcaa
     3541 aacaagtaga cccagatcct aaaattaaag aagaagcgga taaattaatt caaacttata
     3601 gtaggtattt cccctccaaa gaagatattt tctttcatcc tgaattagaa gaagggaaat
     3661 cattttatgt tggaggatgg ataggagaaa ctaccatttg cagaccatat tgatgataca
     3721 aaatttgaag aataaatttc aattgaatta caagaactta acaattattt ttaatatagt
     3781 tgttatgttc tttttcatga taggttgtaa taaagaaaag cctgaaataa ttcaagctat
     3841 tacggacaga gcagctactc caaaccttca tgcaaccgat gttactacta ttatttccga
     3901 ttccggaatt acccgctacc gtatttctgc tccgcagtgg gatatttacg ataaagttcc
     3961 ggagccctat tgggaatttc cttatggtat tcacttggaa cgtttcgata tgaatctgca
     4021 tgtagatgcc aatattcaca gcaaatatgc aaaatattat attaatcaga aactgtggga
     4081 attaaaaggt aatgtaaaag caaccaactt aaaaggagaa ttatttgaaa ctgaacaact
     4141 tttctgggat caaaacagcg aacgaattta ttctgatagt ttgattaaag taacacgtcc
     4201 aactcaaatt atcaatgcca ttggatttga atcggatcag tcaatgaatt attatgtctt
     4261 ccacaaggta aaaggtatat tcccatacga agaatcacaa gattcttttc ctcccaatcc
     4321 gcagcaaaca gaaatttcac cttcaaaat
//
LOCUS       DAOTKS010000109         4345 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_17319_length_4345_cov_4.022844, whole genome shotgun sequence.
ACCESSION   DAOTKS010000109 DAOTKS010000000
VERSION     DAOTKS010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17319_length_4345_cov_4.022844"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="P5236_03205"
     CDS             <1..368
                     /locus_tag="P5236_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRU63402.1"
                     /translation="VKPFRGKVVLVDFWATWCGPCRRANKEMEPLKAQLAGKDLVYVY
                     MTGYTSPENTWRNMITDLKGNHYRMNDAQWEYVSQQKKVGGVPTYIILDREGNQSFYS
                     FGFSCTDTMKRELLKALNQ"
     gene            731..2044
                     /gene="der"
                     /locus_tag="P5236_03210"
     CDS             731..2044
                     /gene="der"
                     /locus_tag="P5236_03210"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRU63403.1"
                     /translation="MGNIVAIVGRPNVGKSTLFNRFTSTRRAIVSSVSGTTRDRQYGK
                     CEWCGTEFSLIDTGGWVVNTSDVFEEEIKRQILLAIEECDVVLFLVDVQSGITDYDLE
                     VAKILRRSDKPVILVANKGDTFEWQYQAAEFYRLGLGDPFLISAINGLGTGDLLDKVI
                     SYFPSKKEMETEENLPRFAVVGRPNVGKSSLINAFLGEERNIVTDIAGATRDSIYTRY
                     NKFGYDFYLVDTAGIRKKTKVNEDVEYYSIVRSIRAIENSDVCILMIDATVGIEGQDL
                     SILSLIQKNKKGLVVCVNKWDLIEDKDSRSVKEYEATIRERFSPFTDFPIIFASALTK
                     QRIFKVIESAMKVYENKNRKIPTAQLNDFLLPLIQNYPPPAWKGKYIKIKYVTQLPNT
                     QIPTFLFFANLPQYVKEPYRRFLENKIRSQWNFTGTPIQLFIRQK"
     gene            complement(2080..3546)
                     /locus_tag="P5236_03215"
     CDS             complement(2080..3546)
                     /locus_tag="P5236_03215"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 domain-containing protein"
                     /protein_id="HRU63404.1"
                     /translation="MLINILSSITAYEPNFATYHLLIKIKLFNRTMKIKSIFLVSLFI
                     ILFSSCGDDLSEIGINIQPSKDFISVYTDTFHLSSQNVFVDYIYSRPDSFLLGTFYDQ
                     KYGITQADILAQVNCPVGFTYPEGSVPDSANIVLRYLTWFGDNYSPVEINIYQMNKAT
                     FSYSQPYPSNIDPADYTDKTIHLGKRIVTAKDASGSKIDTMMIRFKLSDDFVRWFYPS
                     SNKYTSQQEFLEKFKGIYITPNFGTATMFYINQIDLYYYYHYTYVRKALDGITDSTVT
                     VNNVITFPANEEVRQVNRFMHPNREQIIKQREEVNYISSPANVFTKINIPLKKIKQKL
                     DNNINDKYLSINSAVLQVDATEVEDTTLAQPLVKYLLLIKESAMDRFFKNNELPSDTC
                     SILATYSTTKNSDNTYNYYYTFNLASLLTNELKTAGTNAAELPDQLSFLLVPVRIKYN
                     SSGSVTEVKHQFLMSAVTICSGKHPSKPMKMNLVYSGF"
     gene            complement(3509..4321)
                     /locus_tag="P5236_03220"
     CDS             complement(3509..4321)
                     /locus_tag="P5236_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HRU63405.1"
                     /translation="MNQVSKVLYISQEIYPYLPESEMAMMGRYLPQEVQEKGLETRTF
                     MPKFGSINERRNQLHEVIRLSGMNLIIDDTDHPLIIKVASIQPARLQIYFIDNEDYFH
                     RKYTVANENNEEYDDNDERTIFFARGVLETIKKLRWTPDVIHCQGWMSALVPLYIKKA
                     YNDDPFFKHSKVVYAVFNDEFKKPFNSNFGEKLMIDGIQRKHIASHLKNQPIDYVALN
                     KLAIDYSDGVIQASSTINEAVLEYILSTKTNFLAFESNDDYADKYIKFYNSL"
BASE COUNT         1441 a          668 c          802 g         1434 t
ORIGIN      
        1 tggtgaagcc tttccgaggt aaagtggtgc tggtggattt ttgggcaacg tggtgcggtc
       61 cctgccgacg ggcaaacaaa gaaatggagc cgttgaaagc tcaactggcc ggtaaagatt
      121 tggtatatgt ttatatgacc ggatatactt cgcccgaaaa cacctggcgt aacatgatca
      181 ccgacttgaa aggcaatcat tatcggatga acgatgcaca atgggaatat gttagtcaac
      241 agaagaaagt tggaggagtg cccacataca ttattcttga cagagaaggt aatcagagtt
      301 tctactcgtt tggtttttcc tgtaccgaca ctatgaaacg cgaattgttg aaagcgttga
      361 atcagtaaat aaagtataga ggttaagtat ctattcaaaa atgattatga tatagaagaa
      421 aaaaggatgc tgtcattact aaatatgaca ctacttgttg ttaaaaaaat attttgcttc
      481 taaatatttt gagaattttc ctttttattc gaaagattgc gtttaattcg ttgaagaaaa
      541 gacaagtata aaggcttaat ggttaccaaa tgcttaaaag ctgattggaa caacctattc
      601 cttttacaat cattggaaaa ttaaatgtga cgatccttca attctaattg taagacagca
      661 taaattgaaa attatctcgt aactttgtat caaattttgt ttagaaaaag ttaatgagaa
      721 aaaatagaaa atggggaata tcgtagcaat tgttggacga ccaaatgtag ggaaatcaac
      781 tctgtttaat cgatttacca gcacccgccg agccatcgta agttccgtat cgggaactac
      841 gcgcgaccgt caatatggaa aatgtgaatg gtgtggaact gaattttctt tgatcgatac
      901 aggtggttgg gtagtaaata cttctgatgt gttcgaagaa gaaattaaac gtcaaatttt
      961 gctggcaata gaagagtgtg atgtggttct ttttttagtc gatgttcaaa gtggaataac
     1021 agattatgat ttggaagtgg cgaaaatttt acgacgttcc gataagcctg tcattttggt
     1081 tgccaataaa ggtgacactt tcgaatggca atatcaggca gcagaatttt atcgtttagg
     1141 attaggtgat ccttttttga tttccgctat caatggatta ggtacaggtg atttgctgga
     1201 taaagttatt tcttattttc cttcaaaaaa agaaatggaa acagaagaaa acttgccacg
     1261 ttttgcagta gtaggtcgtc ctaatgttgg taaatcatca ttgataaatg cattcttggg
     1321 agaagagcgg aatattgtta ccgatattgc tggtgctact cgcgattcta tttatactcg
     1381 ttataataaa tttggttacg atttttattt ggttgatact gctggcatcc gaaaaaagac
     1441 taaagtaaat gaagatgtgg agtattattc cattgttcgt tctattcgtg ccattgaaaa
     1501 cagcgatgtt tgcattttaa tgattgatgc taccgttggt attgaaggac aggatttaag
     1561 cattttgtca ttgattcaga aaaataagaa aggattggtg gtttgtgtca ataaatggga
     1621 tttgatcgag gataaagata gccgttccgt aaaagaatat gaagcaacca tcagagaacg
     1681 tttttctcct tttacagatt ttcctattat ttttgcttca gctttgacaa aacagcgaat
     1741 tttcaaagtg attgaatcgg ctatgaaagt ttatgagaac aaaaacagaa aaattcctac
     1801 tgcacaattg aatgattttt tgctgccatt gattcaaaat tatcctccac cagcatggaa
     1861 aggaaaatat attaagataa aatatgtgac acaattacct aatactcaaa tacctacttt
     1921 tctctttttt gccaatttac cccaatatgt aaaagaacct tatcgtcgtt ttctggaaaa
     1981 taaaattcgt tcgcagtgga attttacagg aacacctatt caattattta tcaggcaaaa
     2041 ataaagttta gaataaacta aaaaaccctt tagaatttat caaaatccac tataaactaa
     2101 attcattttc atcggtttgg aaggatgctt accactgcaa atagtaacag cgctcattaa
     2161 gaattgatgt tttacttcgg tgacagaacc actgctattg tattttatcc taacagggac
     2221 caatagaaaa cttaattggt caggaagttc cgcagcgttg gttccagcag ttttcaattc
     2281 attggtaagc aaagaagcaa gattaaatgt ataataataa ttataagtgt tatcactatt
     2341 ctttgtagta gaataggtag ctaaaatgga acaagtatca gaaggtaatt cattattttt
     2401 aaaaaatcta tccattgctg attctttgat taacaacaaa tattttacaa gcggttgtgc
     2461 cagtgtagtg tcttccactt ctgttgcatc tacttgaagg acagcactgt tgatagataa
     2521 atatttatcg ttgatattgt tgtccaattt ttgtttgatt ttttttaatg gtatattgat
     2581 tttagtaaac acattagctg gcgaagatat gtaatttacc tcttctcttt gtttgataat
     2641 ttgttcccga tttggatgca taaaacggtt tacttgtctt acttcttcgt tggcaggaaa
     2701 agtaataaca ttgttaacag ttactgttga gtcagtaata ccatccagag ctttgcgaac
     2761 gtaagtataa tgatagtaat aatacaagtc gatttgatta atgtaaaaca ttgttgctgt
     2821 cccaaaattg ggagtaatat atataccttt gaatttttcc agaaactctt gctgactcgt
     2881 gtatttattt gaagacggat aaaaccatct aacaaaatcg tccgacagct taaagcggat
     2941 catcatagta tctattttcg aaccactggc atctttggct gttacaattc ttttacctaa
     3001 atgtatggtt ttatcggtat aatcagcagg gtctatattg gaaggataag gttgagaata
     3061 agaaaaagtt gccttattca tctgataaat atttatttct actggtgaat aattatcgcc
     3121 gaaccatgtg aggtatcgga gaacaatatt agctgaatcg ggaacagaac cttctggata
     3181 ggtaaaaccg acaggacaat ttacttgagc caatatatcg gcttgagtta ttccatattt
     3241 ctggtcataa aatgttccaa gaagaaacga atcaggacgg gaataaatgt aatctacaaa
     3301 aacattttga gagcttaaat ggaaagtatc cgtataaaca ctgataaaat cttttgatgg
     3361 ctgaatattt atacctattt cacttaaatc atcaccacag gaagaaaata aaattataaa
     3421 aagagaaact aagaaaatac ttttgatctt catagtgcga ttgaataatt tgatttttat
     3481 taacaaatga taagtggcaa aattaggttc ataggctgtt atagaactta atatatttat
     3541 cagcatagtc atcgtttgac tcaaatgcaa ggaaatttgt tttggtagac aaaatatatt
     3601 ccaaaacagc ctcattgatg gtagagcttg cctgaataac tccatccgaa taatctattg
     3661 ccaatttatt gagagcgaca taatcaatgg gttgattttt taaatgagag gcaatatgtt
     3721 ttctttgaat accatcaatc attaactttt caccaaaatt actattgaaa ggttttttga
     3781 attcatcatt aaaaactgca taaactactt ttgagtgctt aaaaaatgga tcatcattat
     3841 atgctttttt tatatacaat ggaacgagtg cactcatcca accctgacaa tggataacat
     3901 caggtgtcca ccgtagtttt tttattgttt ccagtacacc acgtgcaaaa aaaatagttc
     3961 tctcatcatt atcatcatat tcttcgttat tttcattggc aacggtatat ttcctatgaa
     4021 aataatcttc attatcaata aaatagattt gtaagcgggc aggctgaata gaagcaactt
     4081 tgataataag tggatgatcg gtatcgtcaa taatcaaatt cattcctgaa agtcgtatta
     4141 cttcgtgaag ttgattacgc cgctcattga tagaaccaaa tttaggcata aatgtgcgag
     4201 tctccaaacc tttttcttga acttcttgtg gaaggtatct tcccatcatt gccatttcag
     4261 actcaggtag ataaggataa atttcctgtg aaatgtacaa tacttttgat acttgattca
     4321 ttattcccaa ctaaaataat tcatt
//
LOCUS       DAOTKS010000110         4329 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_17397_length_4329_cov_4.195835, whole genome shotgun sequence.
ACCESSION   DAOTKS010000110 DAOTKS010000000
VERSION     DAOTKS010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17397_length_4329_cov_4.195835"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..1248)
                     /locus_tag="P5236_03225"
     CDS             complement(22..1248)
                     /locus_tag="P5236_03225"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018462777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HRU63406.1"
                     /translation="MNKIKVNQPIVELDGDEMARVMWKMIKEQLILPYLDLNIQYYDL
                     SIENRDATDDQVTIDAAYAIKKYNVGIKCATITPDEARIKEFGLKKMWKSPNGTIRNI
                     LGGTVFREPIICSNVPRLVPNWEKPIIIGRHAFGDQYKATDFVTKGKGKLKVTFTNEN
                     GESQEWEVYNFNGDGVALTMYNTDESIYGFARACFQAALEKKLPLYFSTKNTILKAYD
                     GRFKDIFQEVYNTEFKDKFQEIGITYEHRLIDDMVASVLKWKGDFIWACKNYDGDVQS
                     DAIAQGFGSLGLMSSYLLTPDGKTMEAEAAHGTVTRHFRLYQQGKETSTNPIASIFAW
                     TRGLSHRGKLDGNQELIDFCHELEQVCIETVESSKMTKDLALLVYGDKITRQDYLSTE
                     EFLSALKENLDKKLSN"
     gene            complement(1279..2634)
                     /locus_tag="P5236_03230"
     CDS             complement(1279..2634)
                     /locus_tag="P5236_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HRU63407.1"
                     /translation="MKEEFLIYKLSETIKETSKIDKELFTKYNVKRGLRNEDHTGVLV
                     GLTRIGDVVGYKRTETGELKPIPGKLYYRGIDVEELVNGIQKENRLGFEETAYLLLSG
                     YLPNNEELETFSHIIYEKMPLEHQATISILNLKGHNIMNILARSVLELYIYDENPDNI
                     SRDNLIKQSLNLIAKFPTIISYAYNVLRHHEKGRSLHVRHPNENFSFAENFLYMIKGK
                     DQYTPLEVQILDLALILHAEHGGGNNSTFSVRVTSSTETDTYSSIAAGIGSLKGPLHG
                     GANLKVMGMIDDMKKNIADWTNPEEIDKYLLKILNKQAYDKSGLIYGVGHAVYTISDP
                     RAVLLKKMARELAKEKGLEKEFNFLELIEERAIKTFMEYKGKDVNKQVCINVDFYSGF
                     VYQAIGLPQEVYTPIFAMARVVGWCAHRIEELNFESKRIIRPAYKNVNENQEYIPLSK
                     R"
     gene            complement(2694..>4329)
                     /locus_tag="P5236_03235"
     CDS             complement(2694..>4329)
                     /locus_tag="P5236_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HRU63408.1"
                     /translation="KDVILKLAGMLTVKGATNAVLEYFGEGTETLSATGKATICNMGA
                     EVGATTSIFPYDKKSEQYLKATGRNSIATMANKLSNYLTADPEVLAHPEDFYDKVIEI
                     NLNEIEPHINGPFTPDAAYPISKFAKIVKEKNYPQKMEVGLIGSCTNSSYEDLSRAAS
                     VFEQAIEKKLKINAELLINPGSEQIRTTAKRDGILEVFTKVGGVIMANACGPCIGQWN
                     RKLIPADRPNSIVTTFNRNFAKRNDGNPNTHAFLASPEIVAALTIAGDLCFNPLIDFL
                     VNEKGEKVKLSEPIGFELPLVGYETKEKGYIAPTGEKLPINIDPHSERLQLLQPFPAW
                     DGKDMIELALLIKVKGKCTTDHISMAGPWLRYRGHLDKISDNLLMGAVNAFNNKTNSV
                     LYPPTGEYMPVSAAARRYKAENIGSVVVAEENYGEGSSREHAAMEPRYLNVKAIIAKS
                     FARIHETNLKKQGMLALTFINKSDYDRIREDDKISILGLTEFSPGKNLKIQLHHSDGT
                     TEQFEVAHSYNQTQIEWFKAGSALNFIKEQFEKGNN"
BASE COUNT         1294 a          815 c          689 g         1531 t
ORIGIN      
        1 ttaaagtttt ctgaccattt tttagttaga aagtttttta tctaaatttt ctttcaaagc
       61 agagagaaat tcttccgtag ataaatagtc ttgtcgcgtg attttatcac cataaaccaa
      121 taatgctaag tctttagtca ttttgcttga ttctactgtc tcaatacaaa cctgttccag
      181 ttcatgacaa aaatcaatta attcttgatt gccatctagt ttgcctcgat gtgataagcc
      241 acgtgtccat gcaaaaatgg aagcgattgg attggtggaa gtttcttttc cttgctggta
      301 aagcctgaaa tgtcgcgtaa ctgtgccgtg agctgcttca gcttccatag ttttaccatc
      361 gggtgttagc aaataagaag acattaatcc caatgaacca aatccttgag caatggcatc
      421 agactgaaca tctccatcat aatttttaca agcccaaatg aaatcacctt tccattttaa
      481 tactgaagct accatgtcat caatcaatcg atgttcgtaa gtgataccaa tttcctgaaa
      541 tttgtcttta aattcagtgt tatatacttc ttgaaaaata tccttaaacc gtccatcata
      601 agcttttaaa atggtatttt ttgtagaaaa gtaaagaggt aatttttttt caagagccgc
      661 ttgaaaacaa gcacgagcaa atccataaat agattcatcc gtgttataca tggttagtgc
      721 tacgccatcg ccattaaaat tataaacttc ccattcttgc gattcaccat tttcattggt
      781 aaaagttacc tttaattttc ctttgccttt ggtaacaaaa tcggtagctt tatactgatc
      841 gccaaaagca tgacgcccaa tgataatcgg tttttcccag tttggaacca aacgaggaac
      901 attggaacaa ataataggtt cacggaaaac cgttccacct aaaatattcc gaatcgttcc
      961 atttggtgat ttccacattt tctttaatcc aaactctttt attcgtgctt cgtcgggcgt
     1021 tatggtagca catttaatac ccacattata tttttttata gcataagctg catcaatagt
     1081 tacctgatcg tccgtagcat cacgattttc aatgcttaaa tcataatatt gaatatttaa
     1141 atcgagataa ggaagaatga gctgttcttt aatcattttc cacattactc tggccatttc
     1201 atcgccatct aattcaacaa taggttgatt tacttttatc ttgttcattc gttttgtatt
     1261 attataagaa tttgttcctt atcttttact taaaggaata tattcttgat tttcattgac
     1321 atttttgtaa gccggacgaa taatacgttt actttcaaaa ttaagttcct caattctgtg
     1381 agcacaccag ccaactacgc gtgccattgc aaagataggt gtataaactt cttgtggcaa
     1441 accaatggct tgataaacaa aacctgaata aaagtcaaca ttaatgcaaa cttgtttatt
     1501 tacatctttt cctttatatt ccataaatgt tttgatggcg cgttcttcaa tcagttccag
     1561 aaaattaaat tctttttcaa gtcctttttc ctttgctaat tcacgtgcca tttttttcaa
     1621 taatactgct cttggatcgc taatggtata aactgcatgt cctactccgt agattaagcc
     1681 cgatttgtcg taagcttgtt tgttcaaaat tttgagcaaa tatttatcta tttcttcagg
     1741 atttgtccag tcagcaatat tttttttcat gtcgtcaatc atgcccatta cttttaaatt
     1801 agcgcctcca tgaagtggac ctttcaaaga accaattcct gcagcaatag aagaataggt
     1861 atcagtttcg gtagaactgg ttacgcgtac actgaaagta gaattatttc caccaccatg
     1921 ttcagcatgc aaaatgagag ctaaatcaag aatttgcact tcaagtggcg tatattggtc
     1981 tttccctttt atcatataca gaaaattctc ggcaaatgaa aaattttcgt ttggatgacg
     2041 tacgtgtaag gaacgtcctt tttcatgatg acgcaataca ttataggcat aagaaataat
     2101 ggttggaaat ttggcaatca gattaagaga ctgtttgata agattgtctc gtgaaatatt
     2161 atccggattt tcatcataaa tataaagttc caatacgcta cgagctaaaa tattcattat
     2221 gttatgtcct ttaaggttca aaatactaat ggtagcttga tgttccaacg gcattttttc
     2281 ataaataata tgtgaaaaag tttccaattc ttcgttgtta ggtaaatatc ctgaaagaag
     2341 taaataagca gtttcctcaa atcccaatcg attttctttc tgaatgccat ttactaactc
     2401 ttccacatca attccacgat aataaagttt accaggaatg ggtttcaatt caccagtttc
     2461 tgttcgttta tagcccacta cgtctcctat acgcgttaag cctaccagca caccagtatg
     2521 atcttcattt cttaatcctc gtttgacatt atattttgta aaaagttctt tgtcgatttt
     2581 actggtctcc ttaatggttt cggagagttt ataaataaga aattcttctt tcattgcttt
     2641 ttttaatttt tgaaaatgaa tatatgtttg ttttatattt ttgtctctga attttaattg
     2701 tttccttttt caaattgttc ttttatgaaa tttaaagcac tgcctgcctt aaaccactca
     2761 atttgagttt gattataact atgtgcaacc tcaaattgtt cagtagtgcc atcactgtga
     2821 tgcaactgaa tttttaaatt tttaccagga gaaaattcgg ttaatcctaa gatactgatt
     2881 ttatcatctt ctctaattct atcataatct gatttgttaa tgaaggtaag agcaagcata
     2941 ccttgttttt ttaaattggt ttcgtgaatg cgagcaaacg atttagctat gatagctttt
     3001 acattcaaat agcgaggctc cattgctgca tgttcgcgtg atgatccttc tccataattt
     3061 tcttcggcta ctaccacact tccaatgttt tctgctttat atcttcttgc tgctgcagaa
     3121 acaggcatgt attcaccagt aggaggataa agaacgctat ttgtcttatt gttgaaagca
     3181 tttacggctc ccattaatag attatccgaa attttgtcca gatgaccgcg ataacgcaac
     3241 cagggacctg ccattgaaat atggtctgtg gtgcatttac ctttgacttt aattaacaaa
     3301 gccaattcta tcatatcttt tccatcccag gctgggaaag gctgtaacag ttgcaacctt
     3361 tcagaatgtg ggtcaatatt gataggcaat ttttccccag taggagcaat ataacctttt
     3421 tcttttgttt catagccaac taaaggaagt tcaaaaccaa tcggttcgct taactttact
     3481 ttttctcctt tttcatttac taagaaatca atcaacggat taaagcataa atcaccggca
     3541 atagttaatg cggcaactat ttcaggagaa gcaagaaaag catgggtgtt gggattacca
     3601 tcgtttcgtt tggcaaaatt tctattgaaa gtagtaacaa tagaattagg acgatcagca
     3661 ggaatcaatt tgcggttcca ttgaccaatg caaggaccgc aagcatttgc cataataact
     3721 ccacctactt ttgtaaaaac ttccaatata ccatcacgtt tagcagtagt tctgatttgt
     3781 tctgaaccag gattgattaa aagttctgca ttaattttca attttttttc aatggcttgc
     3841 tcaaaaacag aagcagctct tgataaatct tcataggaag aattggtaca agaaccaatt
     3901 aatccaactt ccattttttg cggataattt ttttctttca ctattttggc aaattttgaa
     3961 ataggataag ctgcatcagg tgtaaatgga ccattgatat gaggttctat ttcgtttaaa
     4021 ttgatttcaa taactttatc ataaaaatct tctggatgag ccagcacttc tggatcagca
     4081 gttaaataat tactcaattt gtttgccatt gtagcaattg agtttcgccc cgtagccttg
     4141 agatattgtt cagatttttt atcatatgga aatatggaag tagtagcacc aacttcagcc
     4201 cccatattgc aaatggtggc ttttcccgta gctgataagg tttcggtacc ttcgccaaaa
     4261 tattccaaaa cagcatttgt agcacctttc acggtaagca ttccggctaa ttttaaaatt
     4321 acatctttt
//
LOCUS       DAOTKS010000111         4208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_17962_length_4208_cov_4.603178, whole genome shotgun sequence.
ACCESSION   DAOTKS010000111 DAOTKS010000000
VERSION     DAOTKS010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17962_length_4208_cov_4.603178"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="P5236_03240"
     CDS             <1..799
                     /locus_tag="P5236_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRU63409.1"
                     /translation="NQFNYGARLSLNWARNRILNIDDSPNIPDYLKRNGLQVGMKDGF
                     VSLGLFKSDEEAAIYPTVREGAKGGDIRYKDLNGDGKITYDQDRAWIAKSNIPELMGG
                     LDLHASWKGFDFSILFTGAAFCDIALMGYYEGIGWDNTEFTRPFYHDGNSPLSLVENA
                     WTYENPDAKFPRLSTQTRWNNNWASTFWIVDGSYLRIKNLQIGYSLPQKFVNTIGMKS
                     ARVSVSGSNLYTWANKRLNGLDPEAPDVCNGYYPQQKTYSFGINLTF"
     gene            828..2462
                     /locus_tag="P5236_03245"
     CDS             828..2462
                     /locus_tag="P5236_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRU63410.1"
                     /translation="MKKKIFKLTILSFMALLTSCNDWDYLDPVPTDRYSDKMVWQSEK
                     NADLYLNGFYTYVNTYGNFGNGQFGGLLTEGLTDMLKYGSNTAGDGTPNDYAVEPSKI
                     TPDQNLLGVWGDAYNRIRRVNEFLVGLKEYANFSNEIKNRYEAQARFFRAYLYHQLVI
                     RHGTAILLDAPTTNKNNPLSSEEECWNFIENDLDFASRYLPIQWDDANKGRLTKGAAF
                     AFKSRAMLFARRWEKAKAAADSVFALNIYELAPTYEEAFKSYMKTGNKESILEFNYAL
                     PAPSHNFDRQYAPGGDEAEMGGKATPTQEMVEEYELATGGKPDWSTWHSTSGTTETPP
                     YEQLEPRFHASILYNEANWKGRKIEPYVGGKDGFIQYGEEQYPKGKTTTGYYLKKWLD
                     ENNTDLVNIRSTQPWIEIRLAEVYLNHAEACAMLGENNSANQDLATIRARVGLPHTTK
                     TGNDLIEAIRHERKIELAFEGKYYWDLRRWRIAHQVLTGVRFHGLRIEKASNQFTYKY
                     VECDDRDRQFPEKLYRLPIPSNEIANNLAISQIEGW"
     gene            2475..4166
                     /locus_tag="P5236_03250"
     CDS             2475..4166
                     /locus_tag="P5236_03250"
                     /inference="COORDINATES: protein motif:HMM:NF027725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5018 domain-containing protein"
                     /protein_id="HRU63411.1"
                     /translation="MKKYIFLFITSLTLMCLFHSCEEPEEIQRIDTESNSGIMQLVCT
                     FMDGTGEFTVDTTINKYPFQDGDTVKIIVPWFYPPESTNETSIDKMKIKASLPNNVYV
                     SPPFDIIDLTKVTPYTVTSPSGRKITIYITGVRKKSSEAMIKEFELPAVGLKGFILEE
                     QNFIGIVSGGMDLSNQKPKITISYHATISPDTSIAQNFNEPVIYTVTAFDGTQRTYTV
                     SPFTPQKVAYGIRPASARQLWFKSLGEMGLPPADHYSTAIAVSGNYLVINTRNQDNVY
                     VDRFSGEPKGRMQLGPIKGSLTNFFATSDSVGHILICNLVPNAGPNFKVYKFNNVNDA
                     NPVLLIDWPTGGIAMGRKMSVRGNLDGDALITVTQMESKIVHIWEVKNGSIQSAPDGG
                     PTRSISVAFENNWTFQSDAIARSATYPSILFISGYPSSFGAVNADGSVLALYDLIGAG
                     YDSNFVPQSLDYAIFNGAHYLAQTIVALYGGGNNCHLYDVTIPTNLSLAPKDPRLLVF
                     SSPPNVVTDNTNATGDVAIKVSDDGYKMIMYMLITNFGVGAYEFDCIDVDNIFGE"
BASE COUNT         1432 a          673 c          807 g         1296 t
ORIGIN      
        1 caaccaattt aattatggag cacgtttaag tttaaactgg gcacgaaatc ggattttaaa
       61 tattgatgat agccctaata tacccgacta cttaaaaaga aatggtttgc aagtgggcat
      121 gaaagatggc tttgtgtcct taggactttt taaatctgat gaagaagcag ccatctatcc
      181 aactgtacga gaaggagcaa aaggcgggga tatccgctat aaagatttaa atggggatgg
      241 taaaattact tatgatcaag atagagcgtg gatcgcaaaa agcaatattc cagaattaat
      301 gggtggatta gatttacatg cttcctggaa aggatttgat ttttcaatac ttttcacagg
      361 agcagccttt tgcgacattg ctttaatggg ttattatgag ggaataggat gggacaatac
      421 agaatttaca cgtccttttt atcatgatgg aaatagtcca ttaagtttag tagaaaatgc
      481 ctggacatat gaaaatcctg atgcaaaatt tccccgatta tctactcaaa ccagatggaa
      541 taataactgg gcttctactt tctggattgt tgatggctct tatttgagaa taaaaaattt
      601 acaaatcgga tattctctcc cacaaaaatt tgtaaacaca ataggaatga aatctgcaag
      661 agtttctgta tcaggatcaa atctctatac ctgggcaaat aaacgtttaa atggtttaga
      721 ccctgaagca cctgatgttt gtaatggtta ttatccacaa caaaaaactt attcctttgg
      781 gattaattta actttctaat ttttttgaca tctaaaatat tcactatatg aaaaagaaga
      841 tatttaaatt aacaattcta tccttcatgg cacttttaac tagttgtaat gattgggatt
      901 accttgatcc agtgccaacc gatagatatt cagacaaaat ggtatggcaa agtgaaaaaa
      961 atgctgattt atatctaaat ggtttttaca cctatgttaa tacttatggt aattttggga
     1021 atggacaatt tggcggatta ttaacagaag gtttaacgga tatgttaaaa tatggttcaa
     1081 atactgccgg agatgggact ccaaacgatt atgctgttga accttcaaaa ataactcccg
     1141 atcaaaattt attaggtgta tggggagatg cttacaacag aatacggcgt gtcaatgaat
     1201 ttctggtagg tcttaaagaa tatgctaatt tctctaatga aataaaaaat cgatacgaag
     1261 ctcaagctcg tttctttagg gcatatcttt atcatcaatt agtaattcga cacggaactg
     1321 ccattttgct cgatgctcca acgactaata aaaataatcc attgagttct gaagaggaat
     1381 gttggaattt tatcgaaaat gatttggatt ttgctagtcg gtatctacca attcaatggg
     1441 atgatgctaa caaaggaaga cttaccaaag gagctgcttt cgcttttaaa tctcgtgcca
     1501 tgttgtttgc cagacgttgg gaaaaagcaa aggcagcagc ggattcagtt tttgctttaa
     1561 atatttatga attagcacct acttatgaag aggcttttaa atcttatatg aaaactggaa
     1621 ataaagaatc tattttagaa tttaattatg ccttacctgc tccttctcat aactttgacc
     1681 gacaatatgc accaggtggc gatgaagctg aaatgggtgg aaaagccact cctactcaag
     1741 aaatggttga agaatacgaa ttagctacag gtggaaaacc agattggagc acatggcatt
     1801 ctacaagtgg aacgacagaa actcctcctt atgaacaact tgaacctcgt tttcatgctt
     1861 ccatcttgta taatgaggca aattggaaag gaagaaaaat tgagccatat gttggaggta
     1921 aggatggttt tatacaatac ggagaagaac aatatcctaa aggaaaaaca accacaggat
     1981 attacctcaa aaaatggctc gatgaaaaca acactgattt agtaaatatt agaagcacgc
     2041 aaccatggat tgaaattcgt ttagctgaag tatatttaaa tcatgcagaa gcatgtgcaa
     2101 tgttaggaga aaataattca gcgaatcaag atctcgctac tataagggca cgagttggac
     2161 taccccatac tacaaaaaca ggtaatgatc taattgaagc tattaggcat gaacgaaaaa
     2221 tagaattagc ttttgaaggt aaatattatt gggatttacg tcgctggaga attgctcatc
     2281 aagttttaac gggagtacgt tttcatggtt tacggataga aaaagcaagt aatcaattta
     2341 cttataaata tgtggaatgc gatgaccgtg atagacaatt ccctgaaaaa ttgtatcgac
     2401 ttcctatccc atcaaatgaa atagcaaata atttagcaat tagtcaaatc gaaggatggt
     2461 aaaaaacttg aattatgaaa aaatacatat ttttatttat tacaagcctc accttgatgt
     2521 gtctttttca ttcatgtgag gaacccgaag aaattcaaag aatagataca gaaagtaatt
     2581 cgggtattat gcaattggta tgtaccttta tggatggaac cggagagttt actgttgata
     2641 ccacaataaa taagtatcca tttcaagatg gtgatacagt aaaaattatt gttccttggt
     2701 tttatccacc agaaagcact aatgagacaa gcattgataa aatgaaaata aaagcgagct
     2761 tgccaaataa tgtttacgta tcaccgcctt ttgatattat tgatttaaca aaagtaacac
     2821 cgtatacagt tacttctcca agtggtagga aaattactat ttacatcaca ggagttcgta
     2881 agaaaagttc agaagcgatg ataaaagaat ttgaactacc cgctgtagga ttaaaaggtt
     2941 ttattttaga agaacaaaat tttatcggta ttgtaagtgg aggaatggat ttatcaaatc
     3001 aaaaaccaaa aataactatt tcatatcacg ctaccatttc tcccgatact tctatagcac
     3061 aaaatttcaa tgaaccagta atttacactg tcacagcctt tgatggaact caacgaactt
     3121 atacagtgag tcccttcacg ccacaaaagg tagcttatgg cattcgtcca gcaagtgcgc
     3181 ggcaactatg gtttaaaagt ttaggagaaa tggggttacc tcctgctgac cattattcaa
     3241 cggcaattgc tgtgagtggt aattatttgg taattaatac ccgcaatcaa gataatgtct
     3301 atgtagatcg tttttctggg gaaccaaaag gaagaatgca attaggtcct attaagggct
     3361 ctttaacaaa tttctttgcc acaagtgatt cagtaggaca tattcttatt tgcaatttag
     3421 tacccaatgc aggaccaaac ttcaaagtat ataaatttaa caacgtaaat gatgcaaatc
     3481 cagttttatt aatagattgg cctaccggtg gaatagctat gggaagaaaa atgtctgtta
     3541 gaggtaacct tgatggtgat gccttaatta cagttactca aatggaatca aaaatagtgc
     3601 atatttggga agttaaaaat ggaagcattc aatctgctcc tgatggaggt cccacacgtt
     3661 ctatctcagt tgcttttgaa aacaattgga cattccaaag cgatgccata gctcgctcag
     3721 ctacttatcc ctcaatttta tttattagcg gttacccaag tagctttggg gctgttaatg
     3781 ctgatggaag tgtgttagca ctttatgatc ttataggagc aggttacgat tctaatttcg
     3841 ttccacagtc attggattat gcaattttta atggagctca ttatcttgct caaactattg
     3901 tagctcttta tggaggtggt aataattgtc atttgtatga tgtaactatt ccaacaaatc
     3961 tttcattggc acctaaagat ccacgtttat tagtgtttag ttctcctccg aatgtagtaa
     4021 ctgacaatac aaatgcaacc ggtgatgtag caattaaagt ttcggatgat ggttataaaa
     4081 tgattatgta catgcttatt accaattttg gtgtaggagc gtatgaattt gattgtattg
     4141 atgtagataa catttttggc gaataagtcg atcgtaatta acatatctcg tttttttaaa
     4201 acgagata
//
LOCUS       DAOTKS010000112         4178 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_18116_length_4178_cov_5.887703, whole genome shotgun sequence.
ACCESSION   DAOTKS010000112 DAOTKS010000000
VERSION     DAOTKS010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18116_length_4178_cov_5.887703"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1174)
                     /locus_tag="P5236_03255"
     CDS             complement(<1..1174)
                     /locus_tag="P5236_03255"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRU63412.1"
                     /translation="MKFFVRNFWLLLIFNVLATYVAAQNFALKSPRLVIGIMIDGLQQ
                     RHIDQLWNRFDNYGFKQIVEKGAICNNIYYNLLSAGNASDIATVMTGTTPYYHGVTGN
                     HYFSRKNKEIQSIIYDENELGIGTKLTVSAHHLLSSTVTDELMLNFPHQSKTYAVAIN
                     PEEALMLAGHTANSTVWISDETMKWITTTYYSDGLAASADEMNVNDQIRKYASMIWEP
                     MYSPNTYLYPPQGENKKTSFQYKPDSKRKNSSLLLLKNTPTANAVVADLAYRIVNQEK
                     LGTDDYPDFLLLQFTIKTPYEKTSVINSAEKEDIYLQLDKDIQYLLQKIDSKIGMSQV
                     LVFMFGNQTNFHTPIELGNNKIPAGYFNGDRSMALLNTYLMAIYGQEKWVEGYYGKN"
     gene            complement(1233..2354)
                     /gene="hisB"
                     /locus_tag="P5236_03260"
     CDS             complement(1233..2354)
                     /gene="hisB"
                     /locus_tag="P5236_03260"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HRU63413.1"
                     /translation="MKKRVLFIDRDGTILVEPSETYQVDTLEQLEFLPGVIRNLYFIR
                     QKLDFEWVLVSNQDGLGTSAYPQKNFDVVQQKFLQILKNEDITFDKIFIDKSLPEESL
                     PTRKPGIGMLTEYFSEDYDLQNSFVIGDRYTDVELAKNLGCKAIFLNNDEKILEKKKL
                     TEYCVLQTSSWDKIAEFLFAGERTAVVQRTTKETDVYVYLNLDGEGKSSISTGLHFFD
                     HLLEQISKHSGCDLTIQVKGDLEVDEHHTIEDTALALGEAFAKTLSDKRGIERYGFCL
                     PMDDSLSMVAIDFGGRPWLVFDAHFQREYIGDFPTEMILHFFKSFSDAGKMNLYIKAE
                     GENEHHKLESIFKALAKAIKMAVRRDIYHFDLPSTKDAL"
     gene            2535..>4178
                     /locus_tag="P5236_03265"
     CDS             2535..>4178
                     /locus_tag="P5236_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HRU63414.1"
                     /translation="MDWIKSILVDNGVAEIILVYSLVITAGILLGKIKFFGVSLGVTF
                     VLFVGLFAGHLGFKGNPEVIEFIKEFGLILFVYSIGLQVGPSFFSSFKKGGIQLNLLA
                     TSIVLLGVAVVIGLYYLLNEKISMPMLVGVLSGAITNTPGLGAAQEALQHAHHIGQLT
                     EIPPITLGYAVAYPIGVIGIIFSIIILRIIFKIDFEKENEQLRKINESYQENLERITL
                     RVTNKSAIGKQVREIKKIIRYPFIISRLMRNEEFFIPQGDTVLQENDVILLVANTKHI
                     PHIASCIGEKVEMDWKISEEKLVSRRIVVTNSKMNGKTIGSLKLRTLYGVNITRVNRS
                     GIDLLGTPGLALQVGDYVMVVGEIEAIQQVEKLLGNVLQKLNEPPLITIFAGILLGVL
                     FGSIPFYIPGIPMPVKLGLAGGPLIIAILIGRFGYKLKLTTYTTMSANYMLREIGISL
                     FLASVGIAAGDKFVENVISADGLLWMGCAALISFIPLLLIGIISRKFLKMNYFTLIGL
                     LAGSTTDPPALAYANSIAGSDAPAVAYSTVYPLTMFLRVI"
BASE COUNT         1331 a          709 c          705 g         1433 t
ORIGIN      
        1 tatttttccc ataataacct tccacccatt tttcttgacc ataaattgcc attaaataag
       61 tgttcaacaa agccattgaa cgatctccat taaaatatcc cgctggaatt ttattgttgc
      121 ctaattcaat gggtgtatga aaattggtct gatttccaaa cataaaaacc aacacctgcg
      181 acattccgat tttactatca attttttgca acaaatactg aatatcttta tctaattgta
      241 aataaatatc ttctttttca gcagaattga taactgaagt tttttcataa ggtgttttga
      301 tagtaaattg aagtagaaga aaatcaggat aatcgtctgt tcccaatttt tcctgattaa
      361 caattctata ggcaaggtca gccacaacgg catttgcagt tggcgtattt ttcaatagca
      421 aaagggaaga atttttccgt ttactgtcag gtttatattg aaaagaagtt tttttgtttt
      481 ctccttgtgg tggataaaga taagtattag gtgaatacat cggttcccaa atcatagaag
      541 cgtattttct aatctggtca ttcacattca tttcatcagc acttgctgcc aaaccatctg
      601 aataataagt tgtggtaatc catttcatcg tttcgtcact tatccacaca gtactatttg
      661 ccgtatgtcc tgccagcata agagcttctt caggattaat agctactgcg tacgtttttg
      721 attgatgagg aaaatttagc atcaattcgt ctgttactgt gctggacaat aaatgatgag
      781 ccgaaacagt taattttgtt ccaatgccaa gttcattttc atcgtaaata atagattgta
      841 tttccttatt ttttcttgaa aaataatggt ttcctgttac accatgataa taaggagtag
      901 tgcccgtcat aaccgttgca atgtctgaag catttcctgc tgaaagaagg ttgtaataaa
      961 tattgttaca tattgcacct ttttctacaa tttgcttgaa accataatta tcaaatcgat
     1021 tccataactg gtcaatatga cgttgttgca aaccatcaat cattatcccg atgaccaatc
     1081 gcggtgattt taaagcaaaa ttttgagcag ctacataggt tgccaaaaca ttgaaaatta
     1141 acaacaacca aaaattccga acgaaaaact tcatatttta aaaaattaac tttttgattt
     1201 ttaacaaatg aaagacatgt atttttaaac agttataaag catcttttgt tgagggtaaa
     1261 tcaaaatgat aaatatcccg cctgactgcc attttaatag cttttgccaa tgctttgaaa
     1321 atagactcta atttatgatg ttcgttttca ccttcagctt ttatgtaaag attcattttt
     1381 cctgcatcgc taaacgattt gaaaaaatgt aaaatcatct ctgttgggaa gtcaccaata
     1441 tattctcgct gaaaatgggc atcaaaaacc aaccatggtc ttcctccaaa atcaatagca
     1501 accatactta atgaatcgtc cattggtagg cagaaaccat aacgttcaat gcctcgctta
     1561 tcagacaatg tttttgcaaa agcttcacca agtgcaagag ctgtatcttc aatagtatga
     1621 tgttcgtcca cttccaaatc accttttact tgaattgtta aatcacaacc tgaatgtttg
     1681 ctgatttgtt ccagcaggtg gtcaaaaaaa tgaagacctg tagaaataga acttttacct
     1741 tcgccatcca aatttaagta aacatataca tctgtttcct tggtggttcg ttgtacgaca
     1801 gccgtccgtt cgcctgcaaa taaaaattca gcaattttgt cccaactgct ggtctgcaaa
     1861 acacaatatt ctgtcagctt ttttttctct aaaatttttt catcattatt taagaaaata
     1921 gctttacaac ccagattttt agcaagttcc acatcagtat aacgatcacc tataacgaaa
     1981 gaattttgca aatcataatc ttctgaaaaa tattcggtca gcattccaat acccggtttg
     2041 cgagtaggaa gagattcttc aggtaaagac ttatcaataa atatcttatc aaaagtaata
     2101 tcttcgtttt tcagaatttg taaaaatttc tgctgaacaa catcaaaatt tttctgagga
     2161 taggctgagg ttcctaagcc atcttgatta gaaaccagta cccattcaaa atcaagtttt
     2221 tgacgaataa aatataaatt acgaatcaca ccaggcaaaa attcaagttg ttccaacgta
     2281 tctacctgat aagtttcaga tggctctact aaaatggttc catctctgtc aatgaaaagg
     2341 actctctttt tcataaaaaa tcaaacaatt attaaacaat cagaattatt ttttttataa
     2401 ttattctcat tacaaaaata atctattttt atcatgtagc taatatatat ttcaattttt
     2461 aaagagttta aaaattgtgt aattttgcaa ttgtggaaat gtttcccttt taaaacttta
     2521 agtgagtatg tatgatagac tggatcaaat cgattttggt ggataatggt gtagcagaaa
     2581 ttatcctggt atattccctt gtcattaccg caggaatttt actgggaaaa atcaaatttt
     2641 tcggtgtctc tcttggtgta acttttgttt tatttgttgg attatttgct ggtcatttgg
     2701 gcttcaaagg aaatccagaa gtaatagaat tcataaaaga atttggactg attttgttcg
     2761 tatattccat tggattacag gttggtccta gtttcttttc ttcttttaaa aaaggaggca
     2821 ttcagttaaa tttattagct accagtatcg ttttgttggg cgttgctgta gtgatcggtt
     2881 tatattattt gttgaatgaa aaaatttcca tgcctatgct agtaggtgta ttgtcgggag
     2941 ctattaccaa cactccagga cttggtgcag cacaagaagc tcttcagcat gctcaccaca
     3001 tagggcaatt aactgaaata ccacctatta ctttggggta tgcggttgct tatccaattg
     3061 gtgttattgg tatcattttt tccattatca tacttagaat tatttttaag attgattttg
     3121 aaaaagaaaa tgaacaattg cgtaaaataa atgaatccta tcaagaaaac ttggaaagaa
     3181 tcactttaag agtaaccaac aaatcagcca tcggcaagca agtacgagaa ataaaaaaaa
     3241 taatcagata tccttttatt atttcccgat taatgcgtaa cgaagaattt tttatccccc
     3301 agggtgatac tgttttacaa gaaaatgatg tgatcctgtt agtagcaaac acaaaacata
     3361 ttcctcatat tgcttcgtgt attggtgaga aagttgaaat ggactggaaa atttcagaag
     3421 agaagttggt ttctcgccga attgtagtaa ccaatagtaa aatgaatggc aaaacaattg
     3481 gttcgttgaa attaaggaca ctctatggag tcaatataac acgagtaaat cgttcaggaa
     3541 ttgacttatt ggggacgcct ggattggctt tacaggtagg tgattacgta atggtagtag
     3601 gcgaaataga agccattcag caagtagaaa aactattggg aaatgttttg caaaaattga
     3661 atgagccacc tttaattacc atttttgcag gaatcttatt gggcgttttg tttgggagca
     3721 ttccttttta cattcctgga atccctatgc cagtaaaatt gggtttggca ggtggtccat
     3781 tgattattgc tattttaatc ggacgcttcg gctataagtt aaaattgact acttacacta
     3841 cgatgagtgc aaattacatg ttacgtgaaa ttggcatttc actctttctt gctagtgttg
     3901 gaattgcggc aggagataaa tttgttgaaa atgtaatttc tgcggatggc ttgctttgga
     3961 tgggatgtgc tgctttaatt tcttttattc ctttactatt aataggaata atttccagaa
     4021 agtttttgaa aatgaattat ttcactttaa taggattgct tgcgggaagc acaacagatc
     4081 cgcctgcttt agcctatgct aattctatag ctggctcaga tgctcctgca gtagcttatt
     4141 caacagtata tccattaaca atgtttttaa gagtaatt
//
LOCUS       DAOTKS010000113         4145 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_18283_length_4145_cov_4.387531, whole genome shotgun sequence.
ACCESSION   DAOTKS010000113 DAOTKS010000000
VERSION     DAOTKS010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18283_length_4145_cov_4.387531"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1292
                     /locus_tag="P5236_03270"
     CDS             <1..1292
                     /locus_tag="P5236_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase family protein"
                     /protein_id="HRU63415.1"
                     /translation="IFPISTAAEKNYNKKPALMWFDATANFKRLSYPDSIDYYLDKIK
                     KLGFTHTILDVRPICGEVLYKSQYAPQMTEWNGFHRPDFDFVGYFITKAHQLGLQIHA
                     SFNVFVGGHNFYDRGIIYTTHPEWASVVYTPDKGILPITELKNKYSAMINPILPDYQE
                     YILNIFIEFISKYPAIDGIILDRARYDGIMADFSNYSRLKFEEYIGQKIENFPNDIYE
                     WQKDSKGNFYPKEGKHFLKWIEWRAKNIYDFMSLAKEKVKSVNKNISFGVYTGAWYPS
                     YYEVGVNFASKNYDPSKEYKWATSEYKNFGFRELLDLFTVGNYYSTISIEDYLKNHPM
                     IKNETDFKFQQGTWYCVEGSCKKLKKILDGNPFYGGILASQFYNQRKDLTRSIIMNLQ
                     ESDGVMIFDISHIIEKNLWNDVQNGMLESKPLETIKK"
     gene            1323..3206
                     /locus_tag="P5236_03275"
     CDS             1323..3206
                     /locus_tag="P5236_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008651664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRU63416.1"
                     /translation="MKTNKNSIFYFFMSAAFLFSSSSCIKDNVIKTDVLIIGGGTSGI
                     SAGIQSSRLNAKTVIIEESTWLGGMLTAAGVSAVDGNYKLPSGMWGEFKDSLTNYYGT
                     PEALKTGWVSNVLFEPSVGDKIFKNIAAKEKNLKTYYNATLSSLKKKNNKWLAEVNTP
                     NGILSFSAKIIIDATELGDVAKLCGAGYDIGMESKKVTGEPGAPEEANDIIQDLTYVA
                     TLKDYGRDVTIEKPIDYDSTLYLCCCINKFCSNPPQPWSVQTMITYGKLPNDKYMINW
                     PMDGNDCYLNIIEMSTEERIEALKKAKNITMGFIYFMQKELGFNNLGLADDEYPTEDL
                     LPFIPYHRESRRIHGLIRFTENYIREPYSQKYKLYKTNIAVGDYPIDHHHNRYNGQVP
                     LPDLSFYPIPSYGLPLGTLIPKDIQGLIVAEKSISVSNIVNGTTRLQPVVLQIGQAAG
                     ALAALAVKQNKKINEVEVRDVQNTILNYGGYLMPYLDVEKNDSLFLPLQRIGATGILK
                     GEGKSKGWANETWFRANDILLYSELEGLKEVYPDIEINSISSPVILKNALQIIDNIAQ
                     KENIIIKNGIEKASKQIYSKYHFSPFDINKQITRGEMAVLIDRLLDPFNKKKVDIYGN
                     FKN"
     gene            3226..>4145
                     /locus_tag="P5236_03280"
     CDS             3226..>4145
                     /locus_tag="P5236_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 domain-containing protein"
                     /protein_id="HRU63417.1"
                     /translation="MKKTSRRDFLKKAAVASASTIALSQVQAIGSNQQISKLEKEVYP
                     KNRPILNIKKNESLKSKLIVPKENGLKITGTFLDEISHDIPHQNWGEAEWDLDFQYMK
                     AIGIDTVIMIRSGYKKFITYPSKYLLNKGCYMPSVDLLDMFLRLAEKYGMKFYFGLYD
                     SGHYWETGDMSYEIEDNKYVIDEVWKNYGEKYKSFQGWYISGEISRATKGAIEAFHAM
                     GKQCKDVSDGLPTFISPWIEGKKAVAASSTALTKEEAISVQQHEKEWDEIFAGIHDVV
                     DACAFQDGHIDYDELDAFFTVNKKLADKYG"
BASE COUNT         1529 a          602 c          716 g         1298 t
ORIGIN      
        1 ttatttttcc tatatcaacc gctgctgaaa aaaattataa caagaaacct gcactaatgt
       61 ggtttgatgc gacagctaac tttaaacgtt taagttatcc tgattctatt gattattatc
      121 ttgacaaaat aaaaaagtta ggatttactc acaccatctt agatgtacgt cctatttgtg
      181 gtgaagtcct ctacaaaagt caatatgcac cacaaatgac agaatggaat ggctttcata
      241 gaccagattt tgactttgtg gggtatttta taacaaaagc tcatcaatta ggactacaaa
      301 tacatgcttc ttttaatgtt tttgtaggag gacacaattt ttatgatcgt ggcattattt
      361 atactactca ccctgaatgg gcttctgtag tttatactcc tgataaagga attctcccta
      421 ttacggagtt aaaaaacaaa tactctgcca tgattaatcc gattttacct gactatcaag
      481 aatatatttt aaatattttt atagaattta tatcaaaata tccagctatt gatggtatta
      541 ttctcgatag agcaagatat gatggtatta tggcagattt ttcaaactat tctcgtttaa
      601 aatttgaaga atacatagga caaaaaatag aaaattttcc taatgatatt tatgaatggc
      661 aaaaagactc aaaaggtaat ttttatccaa aagaaggtaa acatttttta aagtggatag
      721 aatggagagc caaaaatatt tatgatttta tgtctttagc taaagaaaaa gtaaaatcag
      781 taaataaaaa tatttctttt ggagtttata caggcgcatg gtatccttcc tattatgaag
      841 taggggtcaa ctttgcaagt aaaaattatg atcctagcaa agaatacaaa tgggcaactt
      901 ctgagtataa aaatttcggt tttagagaat tattggattt gttcaccgtt ggaaactatt
      961 attctaccat ttcaattgaa gattatttaa aaaatcaccc gatgataaaa aatgaaacag
     1021 atttcaaatt tcaacaaggg acatggtatt gtgtagaagg ttcttgcaaa aaattaaaaa
     1081 aaattttaga cggaaatcct ttttatggag gaattttggc aagtcaattt tataatcaac
     1141 gtaaagattt aactcgttct attatcatga atttacaaga atctgatgga gtcatgattt
     1201 tcgatatttc tcacattata gaaaaaaatc tatggaacga cgtccaaaat ggcatgttag
     1261 aaagcaagcc tttagagaca ataaaaaaat agatatttta acatctttaa aatctttttc
     1321 ttatgaaaac aaataaaaat tctatatttt acttttttat gagtgcggca tttcttttta
     1381 gttcatcttc ttgcatcaaa gataatgtta tcaaaactga tgtattaatt attggaggag
     1441 gaacaagtgg aattagcgcc ggcattcaat cttcaagatt aaatgctaaa acagtaatca
     1501 ttgaagaatc tacgtggcta ggaggaatgt taactgctgc tggagtaagt gccgtagatg
     1561 gcaattataa attaccaagt gggatgtggg gagaatttaa agattcttta acaaactact
     1621 atggaactcc tgaagcatta aaaacaggat gggtaagcaa tgttttattt gaaccctctg
     1681 taggtgataa aatttttaaa aatatagctg ctaaagaaaa aaatttaaaa acatattata
     1741 acgctacact ttcttcctta aaaaagaaga ataataaatg gttggcagaa gtaaatacgc
     1801 caaatggtat tctttctttt tctgctaaaa tcattattga tgccacagaa ttaggggatg
     1861 ttgctaaact atgtggcgca ggctatgata taggaatgga aagcaaaaaa gtaacaggtg
     1921 aacctggagc accagaagaa gccaatgaca tcattcagga tttaacttat gtagccactt
     1981 taaaagatta tgggcgagat gtcactattg aaaagccaat tgactatgat tccactcttt
     2041 atttatgctg ttgtattaat aaattttgtt caaacccacc acaaccctgg tctgtacaaa
     2101 ctatgattac ttatggtaaa ttgcctaatg ataagtatat gattaattgg cctatggatg
     2161 gcaatgattg ttaccttaac attatagaaa tgtctaccga agaaagaata gaagcactaa
     2221 aaaaggcaaa aaatataaca atgggattca tttactttat gcaaaaagag ttgggattca
     2281 ataatttagg attggcagat gatgaatatc ctacagaaga tttattgcct tttatacctt
     2341 atcatcgcga atccagaaga atacatggtt taattcgttt tacagaaaat tatatcagag
     2401 aaccttattc tcaaaaatat aaattataca aaacaaacat tgctgttggt gattatccaa
     2461 ttgatcatca tcataataga tataacggtc aagtaccact tccagattta agtttttatc
     2521 ctatcccttc ttatggatta ccactcggga cattaattcc caaagacata caaggactaa
     2581 ttgtagctga aaaatctatt tctgtctcca atatagtaaa tggtactacc cgtttgcaac
     2641 cagtagtttt acaaataggg caagcagcag gagctttagc ggcacttgcc gtaaaacaaa
     2701 acaaaaaaat aaatgaggtt gaagttcgtg atgtgcaaaa cacaatatta aattatggtg
     2761 gatatcttat gccttattta gatgtagaaa aaaacgactc tttattttta cctttacaac
     2821 gaatcggtgc tactggcatt cttaaaggag aagggaaaag taagggttgg gctaatgaaa
     2881 catggtttag agcaaacgac attttattat attcagaact ggaaggttta aaagaagtct
     2941 atcctgatat tgaaattaat tctatcagtt ctcctgtcat tttaaaaaat gcattacaaa
     3001 taattgataa tatcgctcaa aaagaaaata tcatcattaa aaatggaatt gaaaaagctt
     3061 caaaacaaat ttattcaaaa tatcactttt ctccttttga tataaataaa caaattacca
     3121 gaggagaaat ggctgtgtta atagatcgat tattggatcc atttaacaag aaaaaagtag
     3181 atatttatgg aaactttaaa aattaactgc taatatattt taaccatgaa aaaaacttca
     3241 agaagagatt tcttaaaaaa agctgctgtt gccagtgctt ctactattgc cttatcccaa
     3301 gtgcaagcta taggttccaa tcaacagata tcaaagttag agaaagaagt ataccccaaa
     3361 aatagaccta tcctcaatat taaaaaaaat gaatcattaa aaagcaagct catcgtacca
     3421 aaagaaaatg gcttaaaaat cacaggaact tttcttgacg aaatttcgca tgacatacct
     3481 caccagaatt ggggtgaagc agaatgggac ctggactttc aatacatgaa agcaattggg
     3541 atagatactg ttataatgat tcgtagtggt tataaaaaat ttattactta cccttcaaaa
     3601 tacttactaa acaaaggatg ttatatgccc tctgttgatt tactggatat gtttctgaga
     3661 ttggcagaaa aatatggtat gaaattttat tttggtctct atgactcagg gcattattgg
     3721 gaaacaggcg acatgtcgta cgaaattgaa gataacaaat acgttattga tgaagtatgg
     3781 aaaaattacg gtgaaaaata taaaagtttt cagggttggt acattagtgg agaaataagc
     3841 agagcaacaa aaggagctat cgaggctttt catgctatgg gaaaacaatg taaagatgtt
     3901 tctgacggac ttcctacttt tatttctcca tggatagaag gcaagaaagc agttgctgca
     3961 agtagcactg cgttgacaaa agaagaagcc atttcggtgc aacaacatga aaaagaatgg
     4021 gacgaaattt ttgccggtat tcatgatgta gttgatgcct gcgcttttca agatggtcat
     4081 attgattatg atgaattgga cgctttcttt actgtaaata aaaagcttgc tgacaaatat
     4141 gggat
//
LOCUS       DAOTKS010000114         4108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_18467_length_4108_cov_3.586479, whole genome shotgun sequence.
ACCESSION   DAOTKS010000114 DAOTKS010000000
VERSION     DAOTKS010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18467_length_4108_cov_3.586479"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2117)
                     /locus_tag="P5236_03285"
     CDS             complement(<1..2117)
                     /locus_tag="P5236_03285"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HRU63418.1"
                     /translation="MKRTNLIFLFFIASIVFSFHLQAVEPYLNTALTFDERVNDLLSQ
                     LTLEEKVSLMLYDSPAIERLGIPAYNWWNECLHGVARSGLATVFPQAIGMAAMWDTDE
                     MFNIATAISDEARAKHHDYVSKGKRGIYQGLTFWSPNINIFRDPRWGRGMETYGEDPF
                     LTAEMAIPFIKGLQGDDPKYLKLIATAKHFVVHSGPEAVRHSFDVHPSEYDLAETYLP
                     HFKRAIQEAGVYSVMCAYQRLNGEPCCGSKYVEDILRNKWGLKGYIVSDCGAISDFYE
                     PDKHHVVNTPEEAAAMAVKAGTDLNCGDTYKHLVNAVKMNLISESDIDVSVKRLLMAR
                     MKLGMFDPDKEVKYAQIPISVVDCEKHRLLALEAARKSMVLLKNDKNLLPFTKNVRKV
                     AVIGPNVDNEEVLLGNYNGYPSMKITPLKGIKAKLPKATIKYALGCRWTDEFPYLTPV
                     SENCFYTDKNLKTKGLNAEYFDNSQWQGTPKHTTIDRKIDFVWETNAPFPDMKYDQFS
                     VRWRGVIVPPVSGIYAIGGEAFSGFKLTLDGKVLTFGQSAHHAKKGFENVKLEKGKPY
                     EIELEYVQNNTEYPYVRLLWDVPGRDLKKEALDLAKKSDLVVLCMGLSPSLEGEEMNV
                     NIDGFYKGDPTDIKLPQVQTELIQEIVKLGKPTVLVLLNGSALAINWENENIPAIVEA
                     WYPGQAGGTAIADVIFGDYNPAG"
     gene            complement(2132..3994)
                     /locus_tag="P5236_03290"
     CDS             complement(2132..3994)
                     /locus_tag="P5236_03290"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRU63419.1"
                     /translation="MYHIKKINIIIVFLIILHLTGFSKDLVSKYELQWEDSTIYTKSY
                     IVDNKNPNASDMNPGTPNLPLLSISKAVQLVKAGEKVVIHSGIYREAIILKNSGKSAR
                     KMISIEAAPGEKVIIKGSRELNVKWIQNLVATDEPLKKDVIYTWLKGIWACQLPDSLF
                     ENDYNPFQLQNIEPKEFDLMPWAWLVKNRAPYNLARGMLFQDGKRMMQIASIDDLRLV
                     AGSFYVAKANKILYIHPFEEKNPNNAFFELVVQSHLLRPAGVGFGYVRLSGLSFEHCA
                     NGFLRTSTGAVTTWGGHHWIIENNTFSQINSSALEFGYYAFENGDDNPLNQPRGGKEE
                     GNTIVRNNIIHDCGTAGIRSYYVINGLIENNTIYNCGWQNAENYWEVAGIKLLQTRNT
                     LIKGNHIYNITGGNGIWLDWDNRYSQIKENIIYNIQTIQGGIFIEASQYPNLIDHNFI
                     WNINGNGVLANDADEVIIINNLIGHTSNSLVRAEVATNRFLNGRALTTNRNKVINNIF
                     IDGGEPISLQGKENIVDYNLYIFTFLPKSFFLNKWIKQSGFDCNSSEIEAKVSFDSDL
                     LLFNFESNMEIPKMPCFELSFKNTEELKKVENCLVLPGPFFNLGHKNKILLKIF"
BASE COUNT         1267 a          878 c          618 g         1345 t
ORIGIN      
        1 cttccggccg gattgtaatc gccaaaaatt acgtcggcaa tcgctgttcc gccggcttga
       61 cccggatacc aggcctcaac aatggcgggt atgttctcgt tctcccaatt aatggccaaa
      121 gcactcccat tgagtaaaac cagcacggtc ggttttccga gtttaacaat ttcctgtatc
      181 aattcggttt gaacttgcgg caatttaata tcagtagggt cgcctttgta aaatccatcg
      241 atattcacat tcatttcttc tccttccaat gaaggactta atcccataca gagtaccacc
      301 aaatccgatt ttttagccaa atccagtgct tcttttttca aatctcttcc cggcacatcc
      361 cacaataatc gaacataggg atattcggta ttgttttgaa catattccaa ctcaatttca
      421 taaggttttc ctttttcaag cttcacattt tcaaatcctt ttttggcatg atgcgcactt
      481 tgtccgaaag tcaaaacttt tccgtccaat gtcaacttaa agccactaaa cgcttcacct
      541 ccaatagcat aaattccact taccggtgga acaatcacac ctcgccaccg aactgaaaat
      601 tggtcgtact tcatatcggg aaatggtgca ttcgtttccc atacaaagtc gatttttcta
      661 tcaatcgtgg tatgttttgg cgttccttgc cattgggaat tatcaaaata ttcggcattc
      721 aatccttttg ttttcagatt cttatcggta tagaaacaat tttcggaaac aggcgtcaaa
      781 taaggaaatt catccgtcca tcggcaaccc aaagcatatt ttatagttgc tttcggcaac
      841 tttgctttga ttcctttcaa aggagttatt ttcatggaag gatatccgtt gtaatttccc
      901 agcaaaacct cttcattatc tacattgggt cctataactg ccacttttcg aacattcttt
      961 gtaaaaggga gcaaattttt atcattcttg agcagcacca tcgattttct tgcagcttcc
     1021 aaagccaaca gccgatgttt ttcgcaatct accacactaa tcggaatttg agcatatttc
     1081 acctctttgt cggggtcgaa catgcccaac ttcatgcgag ccatcagcaa tcgttttacg
     1141 gaaacgtcaa tatccgattc gcttatcaaa ttcattttta cggcattcac caaatgtttg
     1201 tacgtgtcgc cacagttcaa atctgtaccg gcttttacgg ccattgctgc tgcttcttct
     1261 ggagtattca ctacatgatg cttgtctggc tcgtaaaaat cgctgattgc accacaatcc
     1321 gacacaatgt atcccttcaa tccccatttg tttcgcagga tgtcctccac atacttgctg
     1381 ccacagcaag gttcaccgtt cagacgctga taggcacaca tcaccgagta aacaccagct
     1441 tcttgaatag cccgcttgaa atggggcaga taagtttctg ccagatcata ttcgctggga
     1501 tgcacatcaa aggaatgacg aacagcttcc ggaccactgt gcactacaaa atgtttcgcc
     1561 gtggcaatca gtttcaaata tttcggatcg tcgccctgca aacctttgat aaacggaata
     1621 gccatttcag ccgtcagaaa aggatcttcg ccgtaggttt ccatacctct accccaacgt
     1681 ggatcgcgaa aaatattaat attgggactc cagaaagtca atccctgata aatgccccgc
     1741 ttccctttcg acacataatc gtgatgtttg gcacgtgctt cgtcggaaat ggctgtagca
     1801 atattgaaca tttcgtcggt atcccacatg gcagccatcc caatagcttg cgggaagacg
     1861 gttgccaagc ccgaacgtgc tacgccatgc aggcattcgt tccaccaatt ataagcaggg
     1921 attcccaatc gctcgatggc cggcgaatca taaagcatca aactcacctt ttcttccaac
     1981 gttagttgtg aaagcaaatc gttcactctt tcatcaaaag ttaaagctgt gttcaaataa
     2041 ggttcaacgg cctgtaaatg aaacgaaaaa acaatagatg caataaaaaa cagaaaaatc
     2101 aaatttgttc ttttcacaat taaaaaataa attaaaaaat ttttaaaagt attttattct
     2161 tatgacctaa gttaaagaat gggcccggca atactaaaca attttctact ttttttaatt
     2221 cttcagtatt cttaaaagaa agttcaaaac aaggcatttt aggtatttcc atattgctct
     2281 caaaattgaa taataataaa tcagaatcaa aacttacttt tgcttctatt tctgaactat
     2341 tgcaatcaaa tcctgattgt ttaatccact tatttaagaa aaaagactta ggtaaaaaag
     2401 taaaaatata aagattataa tccacaatat tttctttacc ttgtagagat attggttccc
     2461 ctccatcaat aaaaatatta tttataactt tatttcgatt tgtagttaaa gctcttccat
     2521 ttaaaaaacg gtttgtggca acttcagctc taacaagtga atttgaagta tgccctatta
     2581 aattatttat aataattact tcatccgcat cattagccaa aacaccattc ccattaatat
     2641 tccaaataaa attatgatca atcagattag gatactgtga tgcttcaata aaaattcctc
     2701 cttgaattgt ttgtatattg taaataatat tttcttttat ttgagaataa cgattatccc
     2761 aatccaacca aataccatta cctcctgtta tattataaat atgatttcct ttaataaggg
     2821 tattgcgagt ttgtaaaagc ttaattcctg caacttccca ataattttct gcattttgcc
     2881 atccacaatt ataaattgta ttattctcaa tgagtccatt tataacataa taacttctta
     2941 taccggcagt cccacaatca tgaataatat tatttcgaac aattgtgttc ccttcttctt
     3001 tcccacctcg aggttgattt aaaggattat catctccatt ttcaaaagca taataaccaa
     3061 attcaagtgc agaagaatta atttggctaa aagtattatt ttctattatc caatgatgcc
     3121 ctccccatgt ggtaactgca ccagtactgg tgcgtaaaaa accattcgca caatgttcaa
     3181 atgataaacc tgaaagtcga acatacccaa aaccgactcc agcaggtctt aatagatgac
     3241 tttggacaac taattcaaaa aaagcattat ttggattttt ttcttcaaaa ggatgaatat
     3301 ataaaatctt atttgctttt gcaacataaa aagaacctgc taccaacctt agatcatcaa
     3361 ttgaagctat ttgcatcatt cttttgccat cttggaataa cattccgcga gctaaattat
     3421 aaggtgctct atttttgact aaccaagccc atggcattag atcaaattct ttgggttcaa
     3481 tattttgaag ttgaaaagga ttataatcat tttcaaacaa agaatcagga agctgacaag
     3541 cccatattcc tttcaaccac gtataaatta catctttttt taaaggttca tcagtagcaa
     3601 ctaaattttg tatccattta acattaagtt ctctagatcc ttttataatc accttttctc
     3661 cgggggctgc ttcaatacta atcatttttc ttgctgattt tcctgaattc ttcagaatta
     3721 tcgcttcacg ataaataccc gaatgaatta ccactttttc gcctgcttta accaattgaa
     3781 ctgcttttga aatacttaaa agaggtaaat tgggagttcc tggattcata tcagaagcat
     3841 taggattctt attatctacg atataacttt tagtgtatat tgtagaatct tcccactgga
     3901 gttcatattt tgaaactaga tctttggaaa agcctgttaa atgcaaaata atcaaaaata
     3961 ctattataat atttattttt tttatgtgat acattgtttt gcttttgtta ttttacataa
     4021 atttaagtct actctgaaaa atcgattttt aatcggattt tcctttccaa attttgtaaa
     4081 aaatgatcta aaagccaata ccaatcgg
//
LOCUS       DAOTKS010000115         4092 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_18550_length_4092_cov_4.926678, whole genome shotgun sequence.
ACCESSION   DAOTKS010000115 DAOTKS010000000
VERSION     DAOTKS010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18550_length_4092_cov_4.926678"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="P5236_03295"
     CDS             <1..467
                     /locus_tag="P5236_03295"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRU63420.1"
                     /translation="IFAIYGWLPSIFKWLMKIELRKIPLVGTACELAGHIFIDRSNPI
                     AAQESIKKAEKKLKNGVSVVIFPEGTRTRDGKLGKFKLGAFRIATDLSLPIVPITIRG
                     SFERISRSTLKITPGTIEMIIHQPIDVNAYLPNKASELVKLSWEIVHADLEK"
     gene            968..1840
                     /locus_tag="P5236_03300"
     CDS             968..1840
                     /locus_tag="P5236_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRU63421.1"
                     /translation="MSFNIVVLAKQVPDTRNVGKDAMKADGTVNRAALPAIFNPEDLN
                     ALEQALRLKSKFEGSTVKVLTMGPVRAAEILRESLYRGADEGYLLSGREFAGSDTLAT
                     SYALTCAIRKFDKVDIILCGRQAIDGDTAQVGPQVAEKMGFPQITYAEDILEVTENSI
                     TVKRRLERGVEIVRGPLPILITVNGTAAPCRPRNAKLTMKYKHAATPSEKQEMGADYT
                     DLYDTHPYLNLTEWGANDIEHDPRWLGLNGSPTKVKKIENIVFTVKESKRLTANDADI
                     DSLMKELINNHTLG"
     gene            1907..2926
                     /locus_tag="P5236_03305"
     CDS             1907..2926
                     /locus_tag="P5236_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007486135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRU63422.1"
                     /translation="MNNIIVYLEIEDGVVRDISLELLTKGRALAQQLGCKLEAVAIGY
                     QLDNIAKQVFPYGVDKLYVCDDKRFIPYTTLPHASVLINLFKQEKPQIALLGATTIGR
                     DLAPRVSSALFSGLTADCTALEIGDYEDKKAGKVYKNLLYQIRPAFGGNIVATIVNPE
                     CRPQMATVREGVMKKEIYDSKHTGEIHKLQVAKYVSDKDFVIEVLERNIEKSKINLKS
                     APVIVSGGYGVGSAENFKLLYELAEALGGEVGASRAAVDAGFAEHERQIGQTGTTVRP
                     KLYIACGISGQIQHIAGMQESAMVISINSDPNAPINKIADYVITGTIEEVVPKMIKYY
                     KKNTK"
     gene            3087..>4092
                     /locus_tag="P5236_03310"
     CDS             3087..>4092
                     /locus_tag="P5236_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRU63423.1"
                     /translation="MNFFNDNPALKFQLSHPLMQKIVTLKERNFVDKDKYDYASRDFE
                     DAIDNYEKILEIIGEIAGEVIASNAEEIDQTGPTVVNNRVIYAPGTQQNHETLIQAGV
                     YGMALPREYGGLNFAVIPYVMAAEIVSRADAGFANIWGLQDCAETINEFADEDIKREF
                     LPRVCAGQTCSMDLTEPDAGSDLQAVQLKATYNEKDGMWYLNGVKRFITNGDAHIKLV
                     LARSEEGTTDGRGLSLFVVDNKHDNTVKVRRIENKLGIKGSPTCELVFSNTPAKLIGS
                     RRLGLIKYVMSLMNEARLGIGAQSVGLSTAAYNEALSYAKERRQFGKAIIEFPAVFEM
                     I"
BASE COUNT         1419 a          617 c          778 g         1278 t
ORIGIN      
        1 acatttttgc catttatggt tggttacctt ccatttttaa atggttgatg aaaatagaat
       61 taagaaaaat acccttagta ggtactgcct gtgaattggc aggtcacatt tttattgatc
      121 gttcaaatcc gatagcagca caagaaagca taaaaaaagc tgaaaaaaaa ctgaaaaatg
      181 gtgtttctgt ggtgattttt ccagaaggaa cccgtacacg tgatggtaaa ttgggaaaat
      241 ttaaacttgg agcttttcgt atcgctactg atttgtcgtt gcctattgtg cctattacga
      301 ttcgagggag ctttgaacgc atttcacgtt cgacgttgaa aattactcca ggcaccattg
      361 aaatgattat tcatcaacct attgatgtaa atgcttattt acccaataaa gcgtcagagc
      421 tggtaaaact tagctgggaa atcgttcatg ccgacttaga aaaatgaaga aaaaaatgat
      481 ttatgtccaa tttcatttgg gaaactaatg atttatttat ttataataag aacaggtaac
      541 agttaaaaac caactgacaa acaacttaat ctaatgattt taaataggtt atgttatata
      601 tttggttatt tcttttttct tttcctttaa aaaattatat aaaataggtt gtaaaacata
      661 ttttttgact tgtaaatttc aatttttaat acgtattttg caactgttaa ctaaacaaca
      721 caatcttttt aataccttaa aaaactaata cctaattgca cacgcgagac ttgctcaagc
      781 ctcgcgtttt ttatcccctt cattgttgaa aataaaataa aattgcttgt ttcttcaata
      841 aaaactgcta attttgcaaa cttaatttaa acaactcttt gctaactgac tttttatcaa
      901 aacaaagtga tttataagga gtaaggagaa gcgacagaaa ttaatttaat gaaataatat
      961 tattgatatg agttttaaca ttgtagtttt ggcaaaacaa gtacctgata caaggaatgt
     1021 aggaaaagac gctatgaaag cagatgggac tgtgaatcga gcagctttac ctgccatttt
     1081 taatccagaa gatttgaatg cattggaaca agcattacgc ttaaaaagta aattcgaagg
     1141 atcaactgtg aaagttttaa ctatgggtcc tgttcgtgca gcagaaattc ttcgggaaag
     1201 tctttatcgt ggggcagatg aaggatattt attaagtgga cgcgaatttg ctggttctga
     1261 taccctggct acttcgtatg ccttaacttg tgccattcgc aaattcgata aagttgacat
     1321 tattttatgc ggtcgtcaag caattgatgg tgacacagca caggtgggtc cgcaagtagc
     1381 tgaaaaaatg ggttttcccc aaataactta tgcagaagat attttagaag taacagaaaa
     1441 tagtattaca gtaaaacgtc ggttagaaag aggagtagaa atagtaagag gtccacttcc
     1501 tatattaatc actgttaatg gtactgctgc tccgtgtcgt ccacgcaatg caaaacttac
     1561 catgaaatat aaacatgctg ctacaccgtc tgaaaaacaa gaaatgggtg ccgattatac
     1621 cgatttatat gatacccatc cttatctgaa tcttactgaa tggggagcaa atgatattga
     1681 acatgatcct cgctggttag gtttgaacgg ctcaccaact aaagtaaaaa aaattgagaa
     1741 tattgttttt actgtaaaag aaagtaaacg tttaacagct aatgatgctg atattgacag
     1801 tttaatgaag gaattaatta acaatcatac attgggataa tcttttattg attaaactat
     1861 caaatgaaaa caataaactt ataattcttt aatagaaaaa ttgaatatga acaatataat
     1921 tgtgtatttg gaaattgaag atggggtagt gagggatatt agtttggaat tacttaccaa
     1981 aggacgtgct ctcgcacaac aattaggttg caaacttgaa gccgtagcaa taggatatca
     2041 attagataat attgctaagc aagtttttcc ttatggtgta gacaagttat atgtatgtga
     2101 tgacaaaaga tttatacctt atactacttt acctcatgct tcagttttaa ttaatctttt
     2161 caagcaagaa aaaccacaaa ttgcattatt gggagccacg acaataggtc gcgatttagc
     2221 gccacgtgtt tcttctgctt tattcagtgg cttaacagcc gattgcacag ctctcgaaat
     2281 tggagattat gaagataaaa aagcaggcaa agtatataaa aatttacttt atcagattcg
     2341 tcctgctttt ggtggaaaca tagttgctac tattgttaat cctgaatgtc gcccacaaat
     2401 ggctactgtg cgtgaaggtg ttatgaaaaa agaaatatat gattcaaaac atacgggcga
     2461 aattcataag cttcaagttg caaaatatgt ttctgataaa gattttgtta tagaagtatt
     2521 ggaacgtaat attgaaaaat caaaaataaa cttaaaatca gccccagtta ttgtatcagg
     2581 aggctatgga gtaggaagtg ctgaaaattt taaacttcta tatgaattag ctgaagcgtt
     2641 gggaggcgaa gtgggagcat cacgagcagc agttgatgct ggttttgcag aacacgaacg
     2701 tcagattggt cagacaggta ccacagtgag accaaaattg tatatcgctt gtggaatttc
     2761 tggacaaatt caacatattg cgggaatgca ggaaagtgca atggttattt caattaacag
     2821 tgatccaaat gcacctatta ataaaattgc agattatgta attactggaa ccatcgaaga
     2881 agtagtgcct aagatgatta aatattataa aaaaaatacc aaataaataa gtatgataaa
     2941 aatttctgaa aaggattttc tcccgttagt aaaaaatcag aattcatatt aaataagtat
     3001 taaattaata aattactaac taaatttata aaatttattt tagatttttt tatcgtttaa
     3061 actaatcaaa gagaaaaaca attgctatga atttttttaa tgataatcca gctttaaaat
     3121 ttcaactttc gcatccgttg atgcaaaaaa ttgtcacgtt gaaagaacga aattttgtag
     3181 ataaagataa atatgattat gcctcgcgcg atttcgagga tgctattgat aattatgaga
     3241 aaattctgga aattattggt gaaattgctg gtgaagttat cgcttcaaac gccgaagaaa
     3301 tagatcaaac aggcccaact gtagtgaata accgcgtaat ttatgcacca ggcacacaac
     3361 aaaatcacga aactcttatt caagctgggg tatatggaat ggctttacca cgagaatatg
     3421 gaggattaaa ttttgcggta attccttatg taatggctgc tgaaattgtt tcgcgtgccg
     3481 atgctggttt tgctaatatt tggggtttac aagattgtgc tgaaaccatt aatgaatttg
     3541 ccgatgaaga tataaaacgt gaatttttgc cgcgtgtatg tgctgggcaa acttgctcca
     3601 tggatttaac tgaacctgat gccggttccg acctacaagc agttcaactg aaagctactt
     3661 acaacgaaaa agatggaatg tggtatttga atggcgtaaa acgttttatt accaatggtg
     3721 atgctcatat taaactggtt ttggctcgct ctgaagaagg tacaaccgat ggtcgtggat
     3781 tatccttatt tgttgttgat aataaacatg ataacacagt aaaagtacga cgtattgaaa
     3841 ataaattggg aatcaaaggc tcaccgacat gtgaattggt tttcagcaac acgccagcta
     3901 aattaattgg ctctcgtcgt ttagggttaa ttaaatatgt gatgtcacta atgaatgaag
     3961 cacgtttggg tattggagca caatcagttg gactatcaac agcagcttat aatgaagctt
     4021 tatcttatgc aaaagaaagg cgacaatttg gtaaagccat catcgaattt cctgcagtat
     4081 ttgaaatgat tt
//
LOCUS       DAOTKS010000116         4078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_18624_length_4078_cov_4.033806, whole genome shotgun sequence.
ACCESSION   DAOTKS010000116 DAOTKS010000000
VERSION     DAOTKS010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18624_length_4078_cov_4.033806"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="P5236_03315"
     CDS             <1..455
                     /locus_tag="P5236_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HRU63424.1"
                     /translation="LTDEEFLKKISEYFLVEKKGKEIYQPNKLHNFSLYLGGNWYSLT
                     AKEGTYNDEDPIEGLDVTVLSHYILDNILDIKDLRTDKRIDFVGGIRGLGELKKLVDS
                     GKMKAAFALYPVSMKQLIAIADSGNIMPPKTTWFEPKLRSGLVIHELE"
     gene            506..>2139
                     /locus_tag="P5236_03320"
     CDS             506..>2139
                     /locus_tag="P5236_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRU63425.1"
                     /translation="MRFRIFSVILILLAFLLLEGCNTASRIKKADNRFEIGEYYAAGE
                     LYKRCYSQLSSKDKPMRAYVAFQQGECYRLTNYYRAEQAFANSIRNNFSDSIVFLRYA
                     QQLHKNRKYADALKYYKIYLQKDSSNIVAKNGILAIDSIEQWKNTPTRYQINYEKQFN
                     ERKSSSFSPSFMNSSNDVLAFTSTRPMNKKAPPKADPITGLPKNNLLMVKKNAIGKWE
                     KPEFIEGEINTLDSDEGVCSFSSDGKTMYFSRAQQSQEKDMGAEIYYSKRAGGSWSTP
                     QRITIFKDSSISVAHPAIAPDGETLYFVSDHPSGFGGKDIYRTVLEGGECKYVENLGP
                     FINTPGDEMFPTIRIDGTLYFSSNGHPGFGGLDIFKATQRTDSVWQIQNMGIPINSSS
                     DDFGITFEGNAERGYFSSNRNENRGYDAIWSFELPEIIYLLEGKVVDENGQPVPDAKI
                     RIVSNTGVNAYTQTKKDGSYRFKLEKDAEYVMLASARGYLNQQNKLSTMGVQESKTYH
                     VDFKLYPINKPIKIENIFYEFGKWDLTPASEKALQE"
     assembly_gap    2140..2149
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2150..2535
                     /locus_tag="P5236_03325"
     CDS             <2150..2535
                     /locus_tag="P5236_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRU63426.1"
                     /translation="DILKINQLILVQVLKDNPNITIELSSHTDYVGDNQFNKILSEKR
                     AQSVVDYLIKHGIEKERLTAVGYGEEQPVVVDENLVQKYPFLKLGQPLTESYILTLTP
                     EQQEIANQINRRTEFKVLKTTYNLY"
     gene            2564..3358
                     /locus_tag="P5236_03330"
     CDS             2564..3358
                     /locus_tag="P5236_03330"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HRU63427.1"
                     /translation="MKQYLHLLNKVLTEGVYKEDRTGVGTISIFGHQMRFLLEEGFPC
                     LTTKKLHLKSIIYELLWFLKGDTNVKYLQENGVTIWDEWADKNGDLGPIYGYQWRSWP
                     DYKGGYIDQISEAVNTIKNNPNSRRIVVSAWNVAQLPQMKLPPCHILFQFYVVNGTLS
                     LQLYQRSADIFLGVPFNIASYSLLLMMMAQVTGLKAGEFIHTLGDAHIYLNHLDQVKL
                     QLTRIPRPLPTMKINPDVKDIFSFQYSDFELINYDPYPPIKGEVAV"
     gene            3489..3992
                     /locus_tag="P5236_03335"
                     /pseudo
     CDS             3489..3992
                     /locus_tag="P5236_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858889.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
BASE COUNT         1417 a          614 c          721 g         1316 t
ORIGIN      
        1 atttgactga tgaagaattc ttaaaaaaaa tttcagaata ttttctggtt gaaaaaaaag
       61 gaaaagaaat ttatcagcca aataaattac ataatttttc gttatactta ggaggcaact
      121 ggtattctct gacagctaaa gaaggaactt ataatgatga agatccgatt gaaggattag
      181 atgttacggt gttgtcccat tatattctgg ataatatttt ggatattaaa gatttgcgga
      241 ccgacaaacg aattgatttt gtaggaggaa ttcgaggttt aggagaacta aaaaaattgg
      301 tcgacagtgg aaaaatgaaa gcagcatttg ccttgtatcc agtatcgatg aaacaattaa
      361 tagcaatagc cgactcagga aatatcatgc ctcccaaaac tacttggttt gagcccaaat
      421 tacggtctgg attagtaatt catgagttgg aatgacttaa tgaaaattta taaatttcac
      481 ttttctcgct ttttaaatat tattcatgcg ttttagaatt ttcagcgtca tattgattct
      541 tttagctttt ctcttactgg aaggatgcaa tactgcttca agaatcaaga aagctgataa
      601 tcgttttgaa attggagaat attatgctgc tggcgagttg tataaacgtt gctatagcca
      661 gctttcttct aaggataaac ctatgcgagc ttatgtggcc tttcagcaag gtgaatgtta
      721 ccgattgaca aattattatc gggctgaaca agcttttgcc aattccatca gaaataattt
      781 ttccgacagc attgtttttt taagatatgc ccaacaattg cacaaaaata ggaaatatgc
      841 agatgctttg aaatattata aaatatatct tcagaaagat tcttccaata ttgttgcaaa
      901 aaatgggatt ttggcgatag attctattga gcaatggaaa aatactccta ctcgttatca
      961 aataaattat gaaaaacaat ttaatgaacg aaagtcttct tctttcagcc cttcttttat
     1021 gaactcttca aatgatgtac tggcgtttac ttctactcgt cctatgaata aaaaagctcc
     1081 tccgaaagct gatcctatta ctggtttacc taaaaataat ttgttaatgg taaaaaagaa
     1141 tgcgatcggg aaatgggaaa aacctgagtt tatcgaaggt gagattaata ccttagattc
     1201 cgacgaaggt gtttgttcct tcagctcaga tggtaaaact atgtattttt ctcgagctca
     1261 acaatcacag gaaaaagata tgggagctga aatttattat tctaaacgtg caggtggaag
     1321 ttggagcaca cctcaaagaa ttactatctt taaagattca agcatttcgg tggctcatcc
     1381 agcaattgca cccgatggtg aaacacttta ctttgtttct gatcatcctt ccggtttcgg
     1441 tggaaaagat atttatcgaa ctgttttaga aggtggagaa tgtaaatatg tagaaaattt
     1501 aggtcctttt attaacacac cgggagatga aatgttcccg acaataagaa ttgatggaac
     1561 actttatttt tcttcaaatg gtcatcctgg ttttggaggg ctcgatattt ttaaagctac
     1621 tcaacgtaca gacagtgttt ggcaaataca aaatatggga atcccaatta attcttcatc
     1681 tgacgatttt ggaattactt ttgaaggaaa tgccgaacga ggttattttt cttcaaacag
     1741 aaatgaaaat cgcggttatg acgccatttg gagttttgaa ttaccagaaa taatttattt
     1801 attggaagga aaagtagttg atgaaaacgg acagcctgtc ccagatgcaa aaattagaat
     1861 tgtaagtaat acaggagtca atgcttatac acaaactaaa aaagacggtt cgtatcgttt
     1921 taaattggaa aaagatgcag agtatgttat gctggcttct gcacgaggat acctaaatca
     1981 gcaaaataaa ttgtcaacta tgggtgttca agaaagtaaa acatatcacg ttgattttaa
     2041 attatatcca attaataaac ccatcaaaat tgaaaatatt ttttatgaat ttgggaaatg
     2101 ggatttgaca cccgcttctg aaaaagcctt gcaagaattn nnnnnnnnna agatattctt
     2161 aaaataaatc aattaatatt agtgcaagtt ttaaaagata atccaaacat tactattgaa
     2221 ttaagttctc atactgatta tgtgggagat aatcaattca ataaaattct ttcagaaaaa
     2281 agagctcaat ctgttgttga ttatttaatt aagcatggaa tagaaaaaga acgattaaca
     2341 gcagtaggtt atggagaaga acaacccgtt gtagtagatg aaaatttggt tcaaaaatat
     2401 ccatttttaa aactgggaca acctttaact gaaagttata ttttgacttt gacacctgag
     2461 caacaggaaa ttgccaatca aattaatcga cgaacagaat tcaaagtgct taaaacaacc
     2521 tataatttat attgataaaa ataaaatatt tatttcttaa aaaatgaaac aatatttaca
     2581 tttattgaat aaagtactta ccgaaggggt ttacaaagaa gatcgaaccg gagtaggaac
     2641 tatcagcatt tttggacatc aaatgcgttt tttgttagag gaaggttttc cttgcctcac
     2701 taccaaaaaa ttacatttaa aatctattat ttatgaatta ctctggtttt taaaaggaga
     2761 caccaatgtg aaatatctgc aagaaaatgg cgtaaccatc tgggatgaat gggcagataa
     2821 aaatggagat ttaggtccca tttatggcta tcaatggcgt tcctggcctg attacaaggg
     2881 cggatatatc gatcagattt cagaagcagt taataccata aaaaataacc caaattctcg
     2941 gagaatagtt gtaagtgcct ggaatgtggc tcaacttcct caaatgaaat taccaccctg
     3001 tcatatactt tttcaatttt atgtggtgaa tggcacttta agcttgcaac tttaccagcg
     3061 aagtgctgat atttttctgg gagtaccttt taatattgct tcttattcat tgttattaat
     3121 gatgatggca caggttacag gcttgaaagc aggtgaattt attcatacct taggtgatgc
     3181 ccatatttat ctgaatcatc tggatcaagt aaaattacaa ttaactcgca ttcctcgtcc
     3241 tttgcccact atgaagatca atcctgatgt taaagatatt ttttcgtttc agtattctga
     3301 ttttgaactt attaattatg atccttatcc tcccattaaa ggtgaagtag cagtataaaa
     3361 aagaattgat taatttcttt ccagaaaaat caaaaaacaa ttttttttat aaacgttgag
     3421 aactttcata ttttttagta actttgaaaa attgtcttaa cgctttttaa catttaaagt
     3481 gatgcatcat gcctaaaatt tcaatcattg ctgctgttgc tgacaattat gcaattggaa
     3541 atttgaataa acttccatgg catttgccag cagatttgaa atattttaaa cagcttacta
     3601 caggtcatcc tatcattatg ggaaaacgca catttgataa tttgccaaat ggaccgcttc
     3661 caaatagaaa aaacattgtt ttgacttctg ttatctccga aggaatcaca aaaggatatg
     3721 tagaagctaa ttcattagaa gaagctattg aaatatgaaa aaatgatgag gaagtattta
     3781 tcatcggtgg tgtttcagta tttaaacaag ctattgatat tgctgacaga atgtacatta
     3841 cgtgggtaca tgaaaatttt aaggcagatg cctttttccc agaaattaat tttgacgaat
     3901 gggaagaaat atgcagagaa gatcaccctg ctgatgataa aaacccttac gcttatactt
     3961 tctctgttta cgaacgaaaa aaaagaaaat aagattttcc tttttatcct atgcctgatg
     4021 gttttttaat tttaatgtag ctggagaaaa ctaaaaatca actaatgtta aatgagta
//
LOCUS       DAOTKS010000117         4069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_18670_length_4069_cov_5.159940, whole genome shotgun sequence.
ACCESSION   DAOTKS010000117 DAOTKS010000000
VERSION     DAOTKS010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18670_length_4069_cov_5.159940"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /locus_tag="P5236_03340"
     CDS             complement(<1..1286)
                     /locus_tag="P5236_03340"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HRU63428.1"
                     /translation="MKTTKKAKAVGLMFLIIIVSVASSLITSYFTNKNKNNNFNQNLQ
                     VPAAYAKFAEVSSAGETDFTVAAELCVHSVVHVKTKSKAQLSFSPDPFFEFFFGTPRF
                     YQEPPVIEGSGSGVIITNDGYIVTNNHVIDRANQIEVTLNDKRTFDAKVVGTDPNTDL
                     ALIKIDADNLPIIPFGNSDSLKVGEWVLAIGNPFNLTSTVTAGIVSAKARNINIINSK
                     MRIESFIQTDAAVNPGNSGGALVNTKGQLVGINTAIASQTGSYAGYAFAIPTSIVKKV
                     VSDLRQYGVVQRAVLGVEIADITAQLAKEKDLKTLEGAYVANVNPGSAAEKAGIKEGD
                     VIINVNGVNVKSVAELQEQIGRYKPGDKISVTVMRKNEKLKFDNVELKNAQGISSVVS
                     ADKINVEKILGATFKEVPASLKKKLDINYGMQVVSLT"
     gene            complement(1574..2851)
                     /gene="eno"
                     /locus_tag="P5236_03345"
     CDS             complement(1574..2851)
                     /gene="eno"
                     /locus_tag="P5236_03345"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRU63429.1"
                     /translation="MSRIEKIIAREILDSRGNPTVEVDVTLECGVMGRAAVPSGASTG
                     ENEAIELRDGDKNRYSGKGVLKAVANVNDIIAPALIGMCSLEQRSIDKKMIALDGTKT
                     KSRLGANAILGVSLAVAKAAAAYLDVPLYRYIGGTNTYVLPVPMMNIINGGSHSDAPI
                     AFQEFMIRPVGAPTFREGLRMGAEVFHSLKKVLQERNLSTAVGDEGGFAPTLKGVEDA
                     IESILTAIKKAGYKPGKDVMIGLDCASSEFYENGIYNYSKFEGPNGAKRTSAQQVEYL
                     TELVNKYPIDSIEDGMSENDWEGWKLLTEKLGKKIQLVGDDVFVTNVEFLKKGIEMGC
                     ANSILIKVNQIGTLSETLDAVEMAHRAGYTSVISHRSGETEDSTIADIAVATNSGQIK
                     TGSLSRSDRMAKYNQLLRIEEELGEIAVYGYKK"
     gene            3108..>4069
                     /locus_tag="P5236_03350"
     CDS             3108..>4069
                     /locus_tag="P5236_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HRU63430.1"
                     /translation="MHKILIILLMLKQQLQQKLQQKLSPAQIQEIKLLEVTTLELEER
                     IRQELEENPVLEEGPEPELEKEELSDDFEEEKIDEEVNDEIDFEEYLDEDEIPDYKLR
                     INNTSKDDKYEEIPFSAGMTFHDYLMDQLNLIPLTEKEKIIAEFIIGNIDEEGYLRRK
                     PEALIDDLAFQLNIETNEEKIRKIIKIIQQLDPPGVGAYDLQECLLLQLERKPFTPEI
                     DIAKRIVKEYFKEFTRKHFEKLIKGLEIDEEKLKKAMNEIIKLNPKPGSAWSGSVLEK
                     SMTTVVPDFILENKDGELYVHLNNSNIPELRINSTYNSMFQDYMA"
BASE COUNT         1353 a          722 c          638 g         1356 t
ORIGIN      
        1 gtaagtgaaa ctacttgcat accataatta atatccaatt ttttctttaa agaagctgga
       61 acttccttga aggtagcacc taaaattttt tcaacattta tcttatcagc agaaacaaca
      121 cttgaaattc cttgtgcatt ttttaattct acattgtcaa atttcagttt ttcattttta
      181 cgcatcactg taacagaaat tttatcgccc ggtttatagc gtccaatttg ttcctgaagt
      241 tctgctaccg actttacatt tactccattg acgttgataa tcacatcacc ttccttaatt
      301 cctgcttttt ctgcagcact tcctggattt acattagcta cataagctcc ttccaacgtt
      361 tttaaatctt tttcttttgc taattgtgca gtaatatcag caatttctac acccaatact
      421 gctctttgaa ctactccata ttgacgaaga tctgaaacca cttttttcac aatgctggtt
      481 ggaatagcaa aagcataacc agcataagaa cctgtttgtg atgcaatggc ggtattaatt
      541 cctactaact gtcctttagt atttacaagg gctccgccac tgtttcctgg attgacagct
      601 gcgtcggtct gaataaatga ttcaattctc attttggaat tgataatatt gatattacgg
      661 gcttttgcac tgacaattcc tgctgtaaca gtagaagtaa gattaaaagg attgccaatt
      721 gccagtaccc attcacctac tttcagtgaa tcagaatttc caaagggaat aatcggtaaa
      781 ttgtcagcat ctatttttat taaagcaaga tcagtattcg gatctgtccc tactactttt
      841 gcatcaaaag ttcttttgtc atttaacgtt acttcaattt gattagcacg gtcaatgaca
      901 tgattgtttg taactatata accatcattt gtaataatta ctcctgaacc tgaaccttca
      961 atcacagggg gttcttgata aaatcttggc gttccaaaaa agaattcaaa aaaaggatca
     1021 gggctgaaag ataattgagc ttttgatttt gttttaacgt gaaccactga atgcacacaa
     1081 agttctgcag ctactgtgaa atctgtttct cctgcactgg atacttcagc aaattttgca
     1141 tacgcagcag gaacttgaag attctgatta aaattattgt tcttgttttt atttgtaaaa
     1201 taactcgtaa taagactgga agccacactc acaataataa tcaaaaacat caagccaact
     1261 gctttcgctt tttttgttgt cttcatttgt tgttttttta aaagttattt ttaatataaa
     1321 tttttatgat tgagagacaa aaataatacc taaaattgat aatgaactct tgcgtttaaa
     1381 taaattatgt tttatacata acagttttga taaagatttg tttaaaatta attgataaat
     1441 tcattcattg cttgatatta tcaaaacaag agatttttca aatgtttaca aaagcaaaga
     1501 aaaatttaat aaaaaaacca cttttaaagg tggtttcttt ggtttaaaat tgacaggaga
     1561 atgacaattt ttcttatttt ttataaccat acactgcaat ttccccaagc tcttcttcga
     1621 tacgcaacaa ctgattatat ttagccatac ggtcagaacg acttagtgaa ccagttttga
     1681 tttgtcctga attagttgct actgcaatat cagctattgt agaatcttct gtttcgcctg
     1741 agcgatgtga aatgacggat gtatatcccg cacgatgtgc catttcaaca gcatcaagcg
     1801 tctcacttaa agtgccaatt tgatttacct taattaaaat ggaatttgca caacccattt
     1861 caatgccttt tttcaagaat tcaacattgg ttacaaacac atcatcgcca actaattgaa
     1921 tttttttgcc taacttttct gttaaaagtt tccaaccttc ccagtcattt tcgctcatcc
     1981 cgtcttctat agaatcaata ggatatttgt tgaccaattc tgttagatat tcaacttgct
     2041 gagctgaagt gcgttttgca ccattgggtc cttcaaattt agaataatta tagatgccat
     2101 tttcataaaa ttcagaagaa gcacaatcaa gtccaatcat cacatctttt cctggcttat
     2161 aaccagcttt tttaatggca gtcaatattg attcaatggc atcttcaact ccttttaaag
     2221 ttggagcaaa accaccttcg tcccctacag cagtgctcaa atttctttct tgaaggactt
     2281 ttttcaaaga atgaaaaact tcagcgccca ttcggagccc ttcacgaaag gtaggagcac
     2341 ctaccggacg aatcatgaat tcctgaaaag ctatgggggc atcggaatgc gaaccaccat
     2401 tgataatgtt catcatgggg acaggcaaaa cataagtatt tgttccacca atatagcgat
     2461 acaaaggaac atcaagataa gcggcagcag cttttgcaac agctaaagaa actcccaaaa
     2521 tggcattggc gcctaacctt gatttcgttt ttgtgccatc caaagcaatc atttttttat
     2581 caattgatcg ttgctctaat gagcacatgc cgattaatgc tggagcgata atgtcattca
     2641 catttgcaac agcttttaat acacctttgc cagaatagcg atttttgtca ccatcgcgta
     2701 attcaatagc ttcattttcg cctgttgaag cacctgaagg aacagcagca cgtcccatta
     2761 caccacattc caatgttaca tctacttcaa ctgtcggatt gcctcttgaa tccaatattt
     2821 cacgggcaat aattttttca attctactca tgttttttta atttttaatt aatttagaaa
     2881 tattttgata atcatttttt tctgatgcaa agtaacaaaa tgttctcata atattatttg
     2941 aataaatcat taaaatttca aaaaggatgt tttttcgtgg attaaaaata attaatttta
     3001 aaagctattt caaatttcca aaatgattta gaaattaaaa aaatatcaga tattttaact
     3061 ttcacaaata tttatgtaat aaactttttt gcaaaaaact tttatctttg cataaaatcc
     3121 ttattatttt actgatgtta aaacaacaat tacaacaaaa attacaacag aaactttcgc
     3181 ctgcccaaat tcaagaaata aagctactcg aagtgactac tttagagtta gaagaacgaa
     3241 tacgccagga attagaagaa aatccagttc tggaggaagg tccggaaccc gagctcgaaa
     3301 aagaagaact ttctgacgat tttgaagaag aaaaaattga tgaggaagtc aatgatgaga
     3361 ttgattttga agaatatctt gatgaagatg aaattcctga ttataagctt agaattaata
     3421 atacttcaaa agatgacaaa tatgaggaaa ttcctttttc tgcgggtatg acttttcatg
     3481 attatttgat ggatcaattg aacctaattc ctcttacaga gaaagaaaaa attattgccg
     3541 aatttattat aggaaatata gatgaagaag gatatttacg tagaaagcct gaagcactta
     3601 ttgatgattt ggcttttcag ttaaatattg aaacaaatga agaaaaaatc aggaaaatta
     3661 tcaaaatcat tcaacagctt gatcctccag gtgtcggagc ttacgattta caagaatgtt
     3721 tgctgcttca gttagaacgt aaacctttta cgcctgaaat tgatattgcc aaacgtattg
     3781 taaaagagta ttttaaagaa ttcacaagaa aacattttga aaaattaatc aaagggttgg
     3841 aaattgatga ggaaaaacta aaaaaggcaa tgaatgaaat aataaaactt aatccgaagc
     3901 ctggaagtgc ctggagcggc agtgtgctgg agaaatctat gactacggta gtgcctgatt
     3961 ttattttgga aaacaaagat ggagaacttt atgtgcattt aaacaatagc aatattcctg
     4021 agttacgaat taatagtacg tacaattcaa tgtttcagga ttatatggc
//
LOCUS       DAOTKS010000118         4046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_18789_length_4046_cov_4.411927, whole genome shotgun sequence.
ACCESSION   DAOTKS010000118 DAOTKS010000000
VERSION     DAOTKS010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18789_length_4046_cov_4.411927"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="P5236_03355"
     CDS             complement(<1..442)
                     /locus_tag="P5236_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63431.1"
                     /translation="MYSEEAFNKVGKEIEELENKKSEEEQKLNVLKSNICQYTGSDIS
                     ADWSVLIDDLFKKREEIKKEFQEIEAKILSGIITNKTIGKLQEEEDKELLEALNSEEV
                     KNFLFKLTGRYKQLLFNDSDIIISDDYNEFSLKDLSTGAKEQVMI"
     gene            592..1425
                     /locus_tag="P5236_03360"
     CDS             592..1425
                     /locus_tag="P5236_03360"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HRU63432.1"
                     /translation="MNCNININSDLIQGTNKSVFTTLQKSMYEFINNRKWTEMTFTPE
                     ERIECTMNITVKKVEGDNFTAEILVQSRRPVYNSSYTTTLFNFKDNDFTFTYKEFDQL
                     EWNPSVITSNLTAVLSYYAYIIIGYDMDSFSRLGGTSAFQAAENIVNMAQGINLSGWK
                     AFESPRNRYALVSNLLDEAFKPFRNYFYEYHRLGLDMMVDNSANARAKIAEGLHVLRD
                     VNRARPSAIVISTFLDAKTDELINIFSKGTEKEKMLVVEILSDVDPAQTAKYEQILNS
                     K"
     gene            1473..3143
                     /gene="recN"
                     /locus_tag="P5236_03365"
     CDS             1473..3143
                     /gene="recN"
                     /locus_tag="P5236_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018909717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HRU63433.1"
                     /translation="MLKSLYISNFALISEININFESGFSVITGETGAGKSIILGALSL
                     LLGQRADSRLIKEGEEKCVIEAEFDIHDYQLEDFFEKNELDYNSSNCLLRREITVAGK
                     SRAFVNDTPVSLQVLRELGDKLIDIHSQYENLLLSHADYQLQVVDTMAQNEKILENYR
                     ITFFEWKTLQNDFRQLQKEAEKQLAEFDYLQFQFQQLNDAHLSENEQTELEAEFQTLS
                     HTEEIKEELQKAYCFLNEEQMAISLIKNAINSLAHIKNFIPEGENWYQRLQSVEIETK
                     DIADEIISLEERIEFQPDRLQWIENRLNEIYALEKKFKVSTEKELISIRDNIKDQLQK
                     IESFDDELADLQKKINEVYEQLIQQANILRETRIKATESIENYLKQQLSQLGMPYIQF
                     KVEISETFDFTENGKDNVQFLFSANKNQSLQPVAQIASGGEISRLMLSIKSLVAHRAG
                     LPTIIFDEIDTGVSGEIAHKMGQIMHDMSKDMQVIAITHLPQIASKGEYHYRVYKDES
                     GEKAQTHIVPLNQEERVQEIAQMLSGENVTEVSLKNAEELLNINYFNN"
     gene            3238..>4046
                     /locus_tag="P5236_03370"
     CDS             3238..>4046
                     /locus_tag="P5236_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HRU63434.1"
                     /translation="MLQSMTGFGKATCEYNNKKIVIEVKSLNSKQLDVSIKIPNLYRE
                     KELEIRNEISQKLERGKIDFSLFIENAGKETATQFNQTIIEGYYQQINDLSEYLGINP
                     PNNWFDIFLRLPDSLKTEISELDENEWQAVKKAIQQAFDELKAFRMQEGKAIENVFYN
                     KISNIQQLLNEIAPFEAERIEKIKEKLKEELESISDKVDYDKNRMEQELIYYIEKLDI
                     NEEKSRLQNHLNYFIQVLEEEQAQGKKLGFIAQEIGREINTLGAKANHSEI"
BASE COUNT         1453 a          623 c          655 g         1315 t
ORIGIN      
        1 caatcataac ctgttctttt gctcccgtgc ttaaatcttt taaagaaaat tcattgtaat
       61 catctgaaat aattatgtct gaatcattaa acaaaagttg tttatatctg cctgttaatt
      121 taaaaagaaa atttttaact tcttcagaat taagtgcttc taatagttct ttatcttctt
      181 cttcctgtaa tttcccaatt gttttatttg taataattcc tgataaaatt tttgcttcaa
      241 tttcctgaaa ttcctttttt atttcttctc tctttttaaa taaatcatca atcaaaacag
      301 accaatcagc gcttatatca ctaccagtat attggcatat attagatttt aaaacattta
      361 atttctgttc ttcttcactc tttttattct ccagttcttc tatttctttt ccaactttgt
      421 taaatgcttc ttctgaatac acttttccag ggtcttctac cacatattca ttttctctaa
      481 tacccagccc acttaacaca ccactttttt tgtttatttc agcattaagt ttctctcttt
      541 tggacaacat ttcatttatt ttctcttttt caatttgttt ctgctcaaga attgaattgt
      601 aatatcaata ttaattctga cctaattcaa ggcaccaata aatcggtttt tacaacattg
      661 caaaaatcca tgtatgaatt tataaacaat cgcaaatgga cagaaatgac tttcactccc
      721 gaagaacgaa tagaatgtac tatgaacatt accgttaaaa aagtagaagg agacaatttt
      781 acagcagaaa ttcttgtaca atcacgtcgt cccgtttata attcaagcta taccaccaca
      841 ctttttaatt tcaaggacaa cgattttacg tttacctaca aagagtttga tcaactggaa
      901 tggaatccct ctgttattac ttccaattta actgctgttc tgtcatatta tgcctatatc
      961 atcattggtt atgatatgga ttctttttct cgattgggag gcacttctgc ttttcaggca
     1021 gcagagaaca ttgtaaacat ggcacaagga attaatttat ctggatggaa agcttttgaa
     1081 agtccgcgaa atcgttatgc tttggttagt aatttattgg atgaagcttt taaacctttt
     1141 agaaattatt tttatgaata tcatcgttta ggtctggata tgatggtgga taattctgct
     1201 aacgcacgtg caaaaatagc cgaaggattg cacgtcttac gtgatgtcaa tagggctcga
     1261 ccttcggcta ttgttatttc tactttttta gatgctaaaa cagacgaact tatcaatata
     1321 ttttcaaaag gaaccgagaa ggaaaaaatg ctggtagtag aaatcctttc agatgttgat
     1381 cctgcccaaa ctgctaaata tgagcaaatt ttaaatagca agtaaaaagt gaattgaatt
     1441 tttacaaaat taaagtctaa cttgaaatct aaatgcttaa atcgctttat atcagtaatt
     1501 ttgctttaat ttcagaaata aatatcaatt ttgaaagtgg tttttcagtt attacaggtg
     1561 aaacgggtgc tggaaaatcc attattctgg gagcactttc tctcttatta ggacaaagag
     1621 ctgatagtag gcttattaag gaaggagaag aaaaatgtgt gattgaagca gaatttgata
     1681 ttcacgatta tcaattagaa gatttttttg aaaaaaatga actggattat aattcttcca
     1741 attgcctttt aagacgagaa atcactgtcg ctggaaaatc aagagctttt gtaaacgata
     1801 cccctgtttc tttgcaagta ttacgtgaac ttggcgataa actgattgac atccattcac
     1861 aatacgaaaa tttactgcta tcacatgccg attatcaatt gcaagtagtg gatacaatgg
     1921 ctcaaaacga gaaaattctg gaaaattacc gaattacttt tttcgaatgg aaaacactac
     1981 aaaacgattt ccgtcaatta caaaaagaag ctgaaaaaca actggcagaa tttgattatt
     2041 tgcaattcca atttcagcaa cttaatgatg ctcatctttc tgagaacgaa caaactgaat
     2101 tagaagctga atttcaaaca ttaagtcaca cggaagaaat taaggaagaa ttgcaaaaag
     2161 cttattgttt tctgaatgag gaacaaatgg cgatttcctt gattaaaaat gccattaatt
     2221 cgctggctca tataaaaaat tttattcctg aaggtgaaaa ttggtatcaa cgtttacaat
     2281 cagtagaaat tgaaacgaag gatattgccg acgaaattat ttcattggaa gaaagaattg
     2341 aatttcaacc agatagatta caatggattg aaaacaggtt gaacgaaatt tatgctttgg
     2401 aaaagaaatt caaggtatca accgagaaag aacttatttc tatcagagat aatataaaag
     2461 atcaattaca aaaaattgaa tcttttgatg acgaattggc tgatttacag aaaaaaatta
     2521 atgaggttta tgaacaattg attcaacaag ccaacatact tagggaaact cgaataaaag
     2581 ctacggaatc tattgaaaat tacttaaagc aacaactttc tcaactgggt atgccatata
     2641 ttcaatttaa agttgaaatt tcagaaactt ttgattttac agaaaatgga aaagataatg
     2701 tgcaatttct tttctctgca aataaaaatc aatctttaca accagttgct caaattgcgt
     2761 ctggcggtga aatttcacga ttgatgttgt caatcaaatc gttagtggct catcgagctg
     2821 gtttaccaac cattattttt gatgagattg atacaggtgt ttcaggtgaa attgctcata
     2881 aaatgggtca aattatgcac gatatgagca aagatatgca ggtaattgcc atcacccatc
     2941 ttcctcaaat tgcttcgaag ggagagtatc attatcgcgt atataaggat gaatcaggcg
     3001 aaaaagccca gacacatatt gttcctctaa atcaagaaga aagagttcaa gaaattgctc
     3061 agatgttgag tggagaaaat gttacggaag tttctcttaa aaatgcagag gaacttctaa
     3121 atattaatta ttttaataac taataagctg attttaaaat aattaaatca aattgattaa
     3181 tttacttgtt catagaatac ttcaattttt tttcgaatag tttaaaaaat taagattatg
     3241 ttacaatcga tgacgggttt tgggaaagct acatgcgaat ataataacaa aaaaattgta
     3301 atagaagtaa aatcgcttaa tagtaagcaa ctcgatgttt ctattaaaat tccaaatttg
     3361 tatcgggaaa aagaattgga aattagaaat gaaatttctc aaaaactcga aagaggaaaa
     3421 attgattttt ctttgtttat tgagaatgca ggcaaagaaa ccgcaacaca atttaaccag
     3481 actataatag aaggttatta tcaacaaatc aacgacttat cagaatattt aggtattaat
     3541 cctcccaaca attggtttga tatttttcta cgtttacctg atagtttgaa aacagagatt
     3601 tcagaattgg acgaaaatga atggcaagca gtaaaaaaag caatacagca ggcttttgat
     3661 gagttaaagg cttttcgtat gcaagaagga aaagccattg aaaatgtgtt ttataacaaa
     3721 ataagcaata ttcagcaatt gctcaacgaa attgctcctt tcgaagctga acgaattgaa
     3781 aaaattaagg aaaaactgaa agaagaactg gaaagtattt cagataaggt tgattacgac
     3841 aaaaacagaa tggagcagga gttaatatat tatattgaaa aactcgatat caatgaagaa
     3901 aagtctcgtt tgcaaaatca tttaaactat tttattcagg tattggaaga agaacaggca
     3961 caaggtaaaa aattaggatt tattgctcag gaaatcggac gcgaaataaa cacgctgggg
     4021 gcaaaagcaa atcacagcga aatcca
//
LOCUS       DAOTKS010000119         4039 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_18826_length_4039_cov_3.867972, whole genome shotgun sequence.
ACCESSION   DAOTKS010000119 DAOTKS010000000
VERSION     DAOTKS010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18826_length_4039_cov_3.867972"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..1146)
                     /gene="prfB"
                     /locus_tag="P5236_03375"
     CDS             complement(join(39..1082,1084..1146))
                     /gene="prfB"
                     /locus_tag="P5236_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445733.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRU63435.1"
                     /translation="MVTIEQLKDLSERQEALRRYLDIENKQIQLQEEELRTQVPDFWD
                     DPKAAENQLRKVKELKNWLTDYNEVKDAVDELKLAFDFFNEEAISEKELDETYQRALN
                     LVENLEMKNMLSHEEDQLGAVIKIVAGAGGTEAQDWAEMLLRMYQRWCERKGYKWEIA
                     SFQPGEEAGMKSVTLQIEGDKAYGYLKSENGVHRLVRVSPYNAQGKRMTSFASVFVVP
                     LIDDSIEIEINPANLTWDTFRSSGAGGQNVNKVETGVRLHYTFKHPLTGEMSEIVIEN
                     TESRSQFDNKENAIRLLKSQLYELELERRRAEQAKLEANKKKIEWGSQIRSYVFHDRR
                     VKDHRTNYQTSNVQAVMDGEIDDFIKAYLMEFSD"
     gene            complement(1165..2283)
                     /locus_tag="P5236_03380"
     CDS             complement(1165..2283)
                     /locus_tag="P5236_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HRU63436.1"
                     /translation="MKKNISIGIMIFIFLVINSSAFGQQRPKNIIIMIGDGMGLPQIY
                     TAMVANNNHLALEDCTYSGFSKTYSADHFTTDSGAGGTAIACGIKTKNGMIGMGPDSV
                     KVTSILEISEQNQLSTGIVVACAVTHATPASFIAHQVDRDMYEEIAADYLKTDIDVFI
                     GGGRQYFEKRKDKRNLLNELKNKNYQLASTLEEVKKIKSGKLVGLLYENHPSSMPERG
                     NMLPEATMAAIDILDNNPKGFFLMVEGSQIDWAAHDNNTKQIIKEMLDFDQTFQQVLN
                     YAKEKGNTLVIITADHETGGMTLLDGKMGSDSLKAEFSTKNHSGIPVPVFAFGPGAEN
                     FTGFMENTDFKPKIEKLLNLKDNDSAIKIKNNIHKKNQ"
     gene            complement(2296..2616)
                     /locus_tag="P5236_03385"
     CDS             complement(2296..2616)
                     /locus_tag="P5236_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRU63437.1"
                     /translation="MEKLYYSISEVAEMLDVTPSNLRFWEKEFTQLRPKRNEKGTRFY
                     SQKDIQTIKQIKYLVDEQKLTLEGARLKLSQKKDEVAKQQELLERLKGIRNELLKIIQ
                     ALND"
     gene            complement(2630..3913)
                     /locus_tag="P5236_03390"
     CDS             complement(2630..3913)
                     /locus_tag="P5236_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRU63438.1"
                     /translation="MNFKPIIFIGVFLLLFFWQSKTQNLEKISTDSTDISQYNENDTS
                     IIIPFGFISNLDQLLHSWALQRIDTSCSANEEIPLVNDSIYKVRLSQMPCLIEMPFHP
                     IVRSFIELYTVQKRTLMEYMLGMSNYYFPIFEQILDANKLPLELKYLPIIESALNPIA
                     ISRMGAAGLWQFMISTGKMYGLEINTLIDERLDPVKSTKAAARFLKDLYDIYGDWNLV
                     IAAYNCGPGNVNKAIRRAGGKTDYWTIYPYLPAETRGYLPIFIAATYSFHYAAEHNLC
                     PAIIDIPIITDTFTVHQRIHFEQIASVLHIPIEELRWLNPQYRKDIVPGDIKPYTITL
                     PLNYSSAFIDKFDEITAYNADELINNRRNEVTIMKASSPSLGGSGKVTYHKVRKGETL
                     GGIAAKYGVSVSQLKKWNNIRGTLIKPGQRLKIIK"
BASE COUNT         1221 a          753 c          634 g         1431 t
ORIGIN      
        1 aaacttgttt gcagagaaaa aatgaaaaaa gcaaaaaatt aatcggagaa ttccattaaa
       61 taagctttga taaaatcatc aatctcacca tccattactg cctgtacatt ggaagtctga
      121 taattagtgc gatgatcttt tactctccta tcatgaaaaa catagcttct tatttgtgag
      181 ccccattcaa tttttttctt gtttgcttct aattttgctt gttccgcacg tcttcgttcc
      241 aattcaagtt cgtataattg tgatttcagc aatcgaatgg cgttttcttt attatcgaat
      301 tgggaacggc tttctgtatt ttcaattacg atttcgctca tttcgccagt aagtgggtgt
      361 ttaaaagtat agtgtaacct tactcccgtt tctactttgt ttacattttg accaccagca
      421 cctgaagagc gaaaagtatc ccaggtaaga ttggctggat tgatttcaat ttcgatgcta
      481 tcatcaatca aaggaaccac aaagacagaa gcaaaagaag tcattcgttt accttgagca
      541 ttataaggtg aaacgcgaac taatctatga acaccatttt cacttttcaa ataaccataa
      601 gccttatcgc cttcaatttg taaagttacc gatttcattc ctgcttcttc gccaggttgg
      661 aaagaagcaa tttcccattt gtagcctttc cgctcacacc atcgctgata catccgcaaa
      721 agcatttctg cccaatcctg agcttctgtt ccacctgctc cagctacaat ttttatcaca
      781 gctcccaatt gatcttcttc gtgcgaaagc atatttttca tttccagatt ttcaaccagg
      841 ttcaaagcac gctgataagt ctcgtcaagt tctttttcac taatagcttc ttcattgaaa
      901 aaatcaaaag ccaatttcaa ctcatccact gcatctttta cttcattata gtctgtcaac
      961 cagtttttta attcctttac ttttcgcaat tggttttctg cagctttagg atcatcccag
     1021 aagtcaggca cttgggtacg caattcttct tcttgaagtt gaatttgttt gttttcaatg
     1081 tcaaagatac ctcctcaacg cctcctggcg ttctgataaa tcttttaatt gttcaatagt
     1141 aaccataatt taattagttc ctaattattg attcttttta tgaatgttgt tttttatttt
     1201 tattgctgaa tcattatctt ttaaattcaa caatttttct atttttggtt taaaatcagt
     1261 attttccata aaacctgtaa aattttctgc accagggccg aaagcaaaaa caggaactgg
     1321 gatacctgaa tgattttttg tactgaattc agcttttaat gaatctgaac ccattttccc
     1381 gtcaagtaaa gtcattccac ctgtttcatg atcagcagtt ataattacca atgtattacc
     1441 tttttctttt gcatagttta aaacctgctg aaaggtttga tcaaaatcga gcatttcttt
     1501 gataatttgc ttggtattat tatcgtgagc agcccaatcg atctgagaac cttccaccat
     1561 caggaaaaat ccttttggat tgttatccaa aatatcaatg gcagccattg ttgcttcagg
     1621 aagcatattt cctctttcag gcatggaaga aggatgattt tcatacaaca agcctaccaa
     1681 tttgcctgat ttgatttttt ttacttcttc cagtgtagag gcaagttgat aatttttatt
     1741 tttcagttca tttaaaaggt tgcgtttatc tttgcgtttt tcgaaatatt gacgaccacc
     1801 accaataaat acgtcaatgt ctgttttaag ataatcggca gcgatttctt catacatatc
     1861 tcgatcaact tgatgagcaa taaaagacgc cggtgtagcg tgggtcacag cacaagctac
     1921 tacaatgccc gtcgaaagtt gattttgttc agaaatttcg agtatagaag ttacttttac
     1981 ggaatcaggt cccatcccga tcattccatt ttttgtttta ataccacaag caatagctgt
     2041 tcctcctgct ccagaatcgg tagtaaaatg atcggcagaa taagtttttg aaaaaccaga
     2101 ataagtacaa tcttccagtg ccaagtgatt attattggca accatagccg tataaatttg
     2161 tggtagcccc attccatcac cgatcataat aatgatattc tttggtctct gttgaccaaa
     2221 tgccgatgaa tttatcacca aaaagataaa tatcattatt ccaatactaa tatttttttt
     2281 cattttccaa aatgattaat cgtttaaagc ttgtataatt tttaataatt cgtttcgtat
     2341 gccttttaaa cgttcaagga gttcctgttg ttttgctact tcatcttttt tttgagataa
     2401 ttttaatctc gccccttcca gtgttaattt ttgctcatca actaaatatt tgatttgctt
     2461 aatggtctgg atatcttttt gggaataaaa gcgtgttccc ttttcattcc gttttggtcg
     2521 aagttgagtg aattcttttt cccagaagcg taaattggaa ggcgttacgt ctaacatttc
     2581 ggctacttca gatattgaat aataaagttt ttccatatta tgggttactt tatttgataa
     2641 ttttcagtcg ttgtcccggc tttattagag ttcctctaat gttattccat ttttttaatt
     2701 gggaaactga tactccatat ttagctgcaa ttcctcctaa ggtttcacct tttcttactt
     2761 tatgataagt aactttcccc gaaccaccca gcgatggtga agaagccttc ataatagtta
     2821 cttcatttct tctgttatta attaactcgt cggcattata agctgtaatt tcatcaaatt
     2881 tatctataaa agcactggaa taatttaatg gcaaggtaat agtgtaaggt ttaatatcac
     2941 caggtactat gtctttacga tattgtggat tcagccagcg aagttcttca ataggaatat
     3001 gcaaaacaga agcaatttgt tcaaaatgaa ttcgttgatg aactgtaaaa gtatctgtta
     3061 tgatcggaat atctataata gcaggacata agttgtgttc agcagcataa tggaatgagt
     3121 aagtggcagc aataaaaata ggtaaatatc cccttgtttc tgcaggcaaa taaggataaa
     3181 ttgtccagta atctgtttta cctccagctc ttcgaatggc tttgtttaca ttacctggtc
     3241 cacaattata agcagcaatt acaagattcc agtctccata aatatcatat aaatccttta
     3301 aaaaacgtgc tgcagctttg gtagatttca cagggtctaa gcgttcgtca atcaacgtat
     3361 ttatttctaa accatacatt ttaccggtag aaatcataaa ttgccacaat ccagccgctc
     3421 ccattcgaga tattgcaatt ggattcaatg cagattcaat aataggaaga tattttaatt
     3481 ctaaaggtaa tttattagca tccaaaattt gctcaaaaat gggaaagtaa taattactca
     3541 ttcccaacat atattccatt aaagtacgct tttgaacagt atataattct ataaaagagc
     3601 gtacaatggg atggaaaggc atttctatta aacaaggcat ttgcgataaa cgcactttat
     3661 aaatggaatc attaacaaga ggaatttctt cattggctga acaacttgtg tcaattcttt
     3721 gtaaagccca tgaatgtagc aattgatcta aatttgaaat aaatccaaag ggtataataa
     3781 tgcttgtgtc attctcatta tattgtgaaa tatctgtact atcagtggat attttttcaa
     3841 gattttgcgt tttggattgc caaaagaaca acaataaaaa aacacctata aaaattattg
     3901 gtttaaaatt catttttgga aaatattttt ataactcatt tcaaatggaa acaaagttgc
     3961 atccctaatg ttctttgtgc ttctgaatta tcaatcaaaa gagttggtcc gaaacccatt
     4021 gataactcag gggtaatgt
//
LOCUS       DAOTKS010000120         3938 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_19396_length_3938_cov_4.270409, whole genome shotgun sequence.
ACCESSION   DAOTKS010000120 DAOTKS010000000
VERSION     DAOTKS010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19396_length_3938_cov_4.270409"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="P5236_03395"
     CDS             <1..434
                     /locus_tag="P5236_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63439.1"
                     /translation="YLDENIVRMCKTHRMFITQLAGALIAKNDSVRAEKVLDYCNEMI
                     PGTSVRHDYSSLMMGQYYYQLQQPAKGDKILEQIANDCVEYLNWSLQLSPTQQNSITS
                     EMGNNMAILNEVLRIFDEAERKDLANKYFPYYVQYSKHFRM"
     gene            435..1040
                     /locus_tag="P5236_03400"
     CDS             435..1040
                     /locus_tag="P5236_03400"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRU63440.1"
                     /translation="MNLQFPKFLRPFIGKYTWRKLPSSKTIYITFDDGPVPEVTPFIL
                     KLLDEYGIKATFFCVGENVKKYPELYQEVLSRGHQVGNHTYNHLKGCSVSKEEYLNNI
                     EKAARLIDSNLFRPPYGKMKRSQQKALNEKYELILWDVITYDYNTKLSPQKVFNNVKH
                     YTRNGSIILFHDSIKAKNNVLAVLPLAIEFWNKKGYNFGVI"
     gene            1097..2374
                     /locus_tag="P5236_03405"
     CDS             1097..2374
                     /locus_tag="P5236_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HRU63441.1"
                     /translation="MKEAILQYVWKHKLFYQNQLYTVDGEKVEVIDVGKYNVDGGPDF
                     FNSKIKIGNTLWAGNVEIHSQSSDWLKHQHQFDKAYDSVILHVVGEANGEVFRTDGSK
                     IPQLVLTFPPKIETNYETLLNNQQWIPCADKLEQIPSIFIQSWKNTLVVERLEQKVKA
                     IDTLLETTQQHWEEAFYITLARNFGFGTNGQAFEALAKSIPLMVLGKHKDNLFQLEAI
                     LFGQAGLLNVEVTDNYVKDLKKEYHFLQTKFSLQPMDNSQWKLFRLRPYNFPHLRIAQ
                     FASLVHNSSKLFSKIIENPYINELKSLLTCEPSSYWQTHYLFGESSGKRTKKLGSASI
                     NSILINTIVPFLFCYGLKKSNEDLKEKSLQILEQLPSEKNALIKGWKNVGLESETAFD
                     SQALIQLKRNYCEEKKCLFCRIGHKVLTLQMTE"
     gene            2434..>3938
                     /locus_tag="P5236_03410"
     CDS             2434..>3938
                     /locus_tag="P5236_03410"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HRU63442.1"
                     /translation="MTNKKRKRTYVLYFFGIYFFISFFSSCANKAQGPTGGPKDTIPP
                     QIIRSVPQNGALNFAKKQILVDFDENISVEKIAENVIISPPQRKPPEIKAIGKRLIVN
                     LLDTLKPNTTYTIDFGNAIVDLNEKNPLKNYVFSFSTGNEIDSLKIAGTVINAEDLNP
                     ISGIIVGIYDELSDSVFLQKPFLRIGRTDEEGDFSINNVKKNTYRIYALGDVNRDYHF
                     QPGEGLAFTDSLIIPDFRKEEVQDTIWKDSVTIDTIRTVIRTHFLPDDILLRYFKESK
                     KRQYLVKYERKQPYMFTLYFNTSLAEMPHIKPLNFDWEGKHLVQKNNTNDTITYWLTD
                     SICWEKDTLQIEMTYLKTDSLYQLIPTTDTLNIFMRKAISASKSKAQKSPIAALFKQD
                     YKFTTNLTSTFEIYLPIQIRFNSPLANVDLSKIQLSEQKDSLFKPLPVQWHQVDSTQM
                     FYAIDYKWEPQKTYKFEIDSAAFTSIYHQVNKKYSTTFKIRSLDEYASLKIV"
BASE COUNT         1405 a          612 c          647 g         1274 t
ORIGIN      
        1 tctatctgga tgaaaacatc gtcagaatgt gtaaaacaca tagaatgttt attactcaat
       61 tggcaggagc cctaatagca aaaaatgatt ccgttagagc tgagaaagtg ctggattatt
      121 gcaacgaaat gattcccggc acgagcgttc gacatgatta ttcttctctt atgatggggc
      181 aatattatta tcaacttcag cagccagcaa aaggagataa aattttggaa caaattgcca
      241 atgactgtgt ggaatattta aactggagtt tacaattaag tcctactcag caaaacagta
      301 ttaccagcga aatgggaaat aatatggcca tattgaatga agttttgaga attttcgatg
      361 aagctgaacg taaagatttg gcaaacaaat attttccata ttatgttcag tattcaaaac
      421 attttcgtat gtaaatgaat cttcagttcc ctaaattttt acgtcccttt attggcaaat
      481 atacgtggag aaaattgcca tcctcaaaga ctatttatat cacctttgat gatggtcctg
      541 ttccagaagt aacacctttt attttaaaat tattggacga atatggaata aaagcaactt
      601 ttttttgtgt aggagaaaat gtgaaaaaat atcctgaact ttatcaagaa gtactgagta
      661 gaggacatca ggtggggaat catacttata atcatttgaa aggttgttct gtctcaaaag
      721 aagaatatct caataatatt gaaaaagctg ctcgactgat tgacagtaat ttattccgac
      781 caccttatgg aaaaatgaaa agaagtcagc aaaaagctct taatgaaaaa tatgaactca
      841 ttttatggga tgtaattact tatgattata acactaaatt atcacctcaa aaagtattca
      901 ataatgtaaa acattatacc agaaacggtt ccattatttt atttcatgat tccataaaag
      961 caaaaaataa tgtactggca gtattgcctc tggcaattga attctggaac aaaaaaggtt
     1021 ataattttgg tgtgatataa aaatttaatt cagtaatatt ggcaagttgg attaactttt
     1081 agattttcat ttatctatga aagaagccat tttacaatat gtgtggaagc ataaactttt
     1141 ttatcaaaat cagctttaca cggttgatgg agaaaaagtt gaggtaatcg atgtaggaaa
     1201 atataatgta gatggtggtc ctgatttctt taattcgaaa atcaaaatag ggaatacatt
     1261 gtgggcaggg aacgtagaaa ttcattctca atcttctgat tggttaaaac atcagcatca
     1321 gtttgacaaa gcttacgata gtgttattct tcatgtggta ggcgaggcaa atggagaagt
     1381 attccgaact gatggttcta aaattccaca attggttttg actttccccc caaaaattga
     1441 aaccaattat gaaacgttac tcaataatca gcaatggata ccttgtgcag ataaattaga
     1501 gcaaattcct tctattttta ttcaaagctg gaaaaatact ttagttgttg aacggcttga
     1561 gcaaaaagtg aaagcaattg ataccctttt ggaaacaact caacaacatt gggaagaagc
     1621 attttatatt actttggctc gtaattttgg ttttgggaca aatgggcagg cttttgaagc
     1681 cctggcaaaa tcaattccac taatggtatt gggaaagcat aaggacaatc tctttcagtt
     1741 agaagctatt ctatttggtc aggctggatt gctgaatgta gaggtgacgg ataattatgt
     1801 gaaagattta aaaaaagaat atcattttct gcaaacaaaa ttttctcttc aaccaatgga
     1861 taactcgcaa tggaaattat ttcgcttgcg tccctataat ttccctcatc tacgaattgc
     1921 acaatttgct tcgttggtac acaactcttc aaaattattt tcaaaaataa tagaaaaccc
     1981 ctacattaat gaattaaagt ctttattgac atgtgaacct tcttcttatt ggcaaacaca
     2041 ttatctgttt ggagaatcaa gtggaaaacg tacgaaaaaa ttagggtcag cttctattaa
     2101 cagcatttta atcaatacta ttgttccttt cttgttttgt tatggattaa aaaaaagcaa
     2161 cgaagatttg aaggaaaaat cattacaaat tttagaacaa ttaccttctg aaaaaaatgc
     2221 attgattaaa ggttggaaaa atgtgggact tgaatctgaa acagcttttg attcacaagc
     2281 cctgattcaa ttaaaaagga actattgtga agaaaaaaaa tgtttgtttt gcagaattgg
     2341 tcataaagtt ttaacgcttc aaatgactga gtaatatttt taaagttctg gtcttcaatt
     2401 ttgaaaagac aaagcaaagt taaattcaat taaatgacaa ataaaaaaag aaaaagaaca
     2461 tatgtccttt attttttcgg aatttatttt ttcatttcgt ttttttcttc ctgtgctaat
     2521 aaagctcaag gtccaactgg cggtccaaaa gatactattc ctcctcaaat aattcgttct
     2581 gttccacaaa atggagcttt gaactttgca aaaaaacaaa ttctggttga ctttgatgaa
     2641 aatatcagtg ttgaaaaaat tgctgaaaac gtaattattt cacctcctca acgcaaacct
     2701 cctgaaatta aagctattgg gaaaagattg attgttaatt tattagatac tttaaaacct
     2761 aacactactt acactattga ttttggtaat gccattgttg atttaaatga aaaaaatcct
     2821 ttaaaaaatt atgtattttc attttcaact ggcaatgaaa ttgattcttt aaaaattgca
     2881 ggaacagtta ttaatgcaga agatctgaat cctatttcag gcatcattgt aggtatttat
     2941 gatgaattgt ccgattctgt ttttcttcaa aaaccgtttc ttcgtattgg tcgaactgac
     3001 gaagaaggag atttttcaat taataatgtg aaaaaaaaca cttatcgaat ttatgcctta
     3061 ggagatgtta accgagatta ccattttcaa ccaggagaag gattggcatt taccgattca
     3121 ttaattattc cggactttag aaaagaagaa gtgcaagata ccatctggaa agattcggta
     3181 accattgaca caattcggac tgtaattcga actcattttt tacccgatga tatacttttg
     3241 cgatatttta aagaaagcaa aaaacgacaa tatctggtaa agtatgagcg aaaacaacct
     3301 tacatgttca ctttgtactt caatacttcg cttgcagaaa tgccccacat aaaaccgctt
     3361 aattttgatt gggaggggaa acatttagtt caaaaaaaca acaccaatga caccataact
     3421 tattggttaa cagattcaat atgttgggag aaagatactt tacaaataga aatgacctat
     3481 ttaaaaaccg attcgttgta tcaattgatt ccaactaccg atactttaaa tatttttatg
     3541 agaaaagcta taagtgcctc aaaatcaaaa gctcaaaaat cacctattgc agctttattt
     3601 aaacaggatt ataaatttac gaccaatcta acgagtactt ttgaaattta tcttcctatt
     3661 cagattcgtt ttaattcccc tttagcaaat gtagatttat ctaaaatcca attaagtgaa
     3721 caaaaagatt ctttattcaa gcctttgcct gttcagtggc accaggtaga ttctacccaa
     3781 atgttctatg ccattgatta caaatgggaa cctcaaaaaa catataaatt tgaaattgat
     3841 tcagcggctt ttaccagcat ttatcatcag gtcaacaaaa aatattctac aactttcaaa
     3901 attcgttctt tggatgaata tgcttctcta aaaattgt
//
LOCUS       DAOTKS010000121         3917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_19545_length_3917_cov_3.701968, whole genome shotgun sequence.
ACCESSION   DAOTKS010000121 DAOTKS010000000
VERSION     DAOTKS010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19545_length_3917_cov_3.701968"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1225
                     /gene="hisD"
                     /locus_tag="P5236_03415"
     CDS             <1..1225
                     /gene="hisD"
                     /locus_tag="P5236_03415"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007480415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRU63443.1"
                     /translation="RALFETVQKILNKVHKDGDNAVKEYTALFDKVSLDTLEVTKEEI
                     NEAEKLVPKELKEAIEMARRNIWKFHSAQQTVFKEIQTSPGVNCWQKALPIEKVGLYI
                     PGGTAPLFSTVLMLGIPAKIAECKEIILCSPPTSNGKIHPTVLYAAKVVGIHRIFKIG
                     GVQAIAAMAYGTETVPKVYKIFGPGNQYVTAAKQLVSMEGVAIDMPAGPSEVAIIADE
                     SANPVFVAADLLSQAEHSVDSQCVLITTSEKLIQPVLDEIERQTKQLLRKDIIQQSLE
                     NARIILVKSIEDAINMINEYAPEHLIIATRFPQEVSLNIVNAGSVFLGNYAPESAGDY
                     ASGTNHTLPTNGYAKSYNGVNLDSFLRKITFQYLTRDGLVNLSNAIILMAENEGLDAH
                     SNAVKVRLDENNLNF"
     gene            1256..2290
                     /gene="hisC"
                     /locus_tag="P5236_03420"
     CDS             1256..2290
                     /gene="hisC"
                     /locus_tag="P5236_03420"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRU63444.1"
                     /translation="MNINELLRENIKKLEPYSCARNDFKGEASIYLDANENPFNDPYN
                     RYPDPLQAELKEQFSKIKNISTSNIFLGNGSDEAIDLLYRAFCEPRIDNVVAIEPTYG
                     MYRVCAEINDVEFRKVLLNKKFDIEAYKLMDATNERTKIIWLCSPNNPTGNSLDGEEI
                     RKILKWFRGIVVIDEAYIDFSNKESFLKEISQYPQLVILQTMSKAWGNAAIRLGMAFA
                     SEEIIQVLNKIKYPYNVNFLTQQHALKVLQDPFQMKKWVSILLEEREKLRNELSKLAI
                     VEEIFPSDANFLLVKVKDANMIYQYLIKKGIVVRNRHNMPLCTNCLRITVGTPDENNV
                     LINTLKKLYI"
     gene            2349..2630
                     /locus_tag="P5236_03425"
     CDS             2349..2630
                     /locus_tag="P5236_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63445.1"
                     /translation="MVRKLVLDVENKIKANNVTVVKSEAIIRGQNVNGIEIICNGKKY
                     YSANLLICTGSRTAIPPICRLSEAGDSMVTNHEMLKLKAQPPSSFSRWK"
     gene            3041..>3917
                     /locus_tag="P5236_03430"
     CDS             3041..>3917
                     /locus_tag="P5236_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRU63446.1"
                     /translation="MKNIFIYFFFFFLTISLNFLFCPKIYAEWNSFIINYDKNLYGKG
                     SQTWQIVPYDTRWVYFANKNGLLEFNGILWNLFPLNTGSDARSVYPSKRQQRIYVGGI
                     NEFGYFEPNEKGKLNYICLSDSFDIANLQIGNIWKIYENDNILYFQGDNKIIKYLNGK
                     FFPIEAHRKIECSDLVNGVIYIGTEEGVWVLVGNTFSPLQGAESLISKRIRGIMPYGK
                     GILIVTAYDGLYYYDEHTLKPYLTGFENFMKQNEVFCAASSEQLIALGTIHGGILLIN
                     NQNHTVKYFNENNGLQ"
BASE COUNT         1364 a          588 c          696 g         1269 t
ORIGIN      
        1 aagagctttg tttgaaactg ttcagaaaat tttaaacaaa gtgcacaaag atggtgataa
       61 tgctgtaaaa gaatatactg ctttgtttga taaagtttct ctcgatactt tagaagtgac
      121 aaaagaagaa ataaatgaag cagaaaagct ggttccaaaa gaattaaagg aagccattga
      181 aatggcacgc agaaatatct ggaagtttca ttctgctcag cagacagttt ttaaagaaat
      241 acaaacttcc cccggcgtta actgctggca aaaagctcta cctattgaaa aagttggttt
      301 atatattcct ggtggcacag ctcctttatt ctcgacagtt ttaatgctgg gcattccagc
      361 caaaatagct gaatgtaaag aaatcatact ttgttcacca ccgacatcaa atgggaaaat
      421 tcatcctact gttttgtacg cagcaaaagt ggtgggcatt catcggatct tcaagattgg
      481 tggtgttcaa gcaattgcag ctatggctta tggaacagaa acagtgccga aagtttataa
      541 aatttttgga cctggcaatc agtatgtcac cgcagctaag cagttagttt caatggaagg
      601 agtagctatt gatatgcctg caggtccctc tgaagttgcc attattgccg acgaaagtgc
      661 aaatcctgtt tttgtcgctg ctgatttact atcgcaagcc gagcatagtg ttgacagcca
      721 atgcgttttg attaccactt cagaaaaatt aattcagcct gttttagatg aaattgaacg
      781 gcaaacaaaa caacttctta gaaaagatat catccaacaa tcattggaaa atgccagaat
      841 tattctggta aaatcaatag aagatgctat taatatgatt aatgaatatg cacctgaaca
      901 tttaattatt gctactcgtt ttccacaaga agtatcttta aatattgtta atgcaggttc
      961 tgtcttttta ggtaattatg ctcccgaaag tgcaggtgat tatgcctcag gcacaaatca
     1021 tacacttcct accaatggtt atgcaaaaag ttataatggt gtaaacttag atagtttttt
     1081 aagaaaaatc acttttcaat atcttaccag agatggtttg gtaaatttaa gcaacgctat
     1141 tattttaatg gctgagaatg aaggacttga cgctcacagc aatgccgtaa aagttcgttt
     1201 agatgaaaat aatttgaatt tttaaaaaat gctttaatga ttagatttat aaaatatgaa
     1261 tatcaatgaa ttattaagag aaaacataaa gaaacttgaa ccttattcat gtgcaaggaa
     1321 tgattttaaa ggcgaagctt ccatctatct tgatgcaaat gaaaatccat ttaatgatcc
     1381 ttataatcgt tatcctgatc ctttacaggc agaattgaag gaacaatttt caaaaataaa
     1441 aaacatatct acctccaata tttttttagg aaacggtagt gatgaagcta ttgatttgct
     1501 ttatcgtgct ttttgtgaac ctcgtattga taacgtagtt gctatcgaac caacttatgg
     1561 aatgtataga gtttgtgctg agataaatga tgttgaattt cgaaaagttc ttctaaataa
     1621 aaagtttgac attgaagcat ataaattaat ggacgctact aatgaacgga caaaaattat
     1681 ctggctatgt tctcccaata atcctacagg taatagttta gatggggaag aaataagaaa
     1741 aattcttaaa tggtttcgtg gaatagtggt aattgatgaa gcatacattg atttttctaa
     1801 taaagaaagt tttctaaaag aaatctctca atatcctcaa cttgttattc ttcaaaccat
     1861 gtcaaaagcc tggggcaatg ctgctataag gttaggaatg gcttttgcct cagaagaaat
     1921 aattcaggta ttaaacaaaa tcaaatatcc ttataatgtt aattttctta ctcagcaaca
     1981 tgctctaaaa gtccttcaag atccttttca aatgaaaaaa tgggtaagca tactgttgga
     2041 agaacgtgaa aaactacgaa atgaattaag taaattagcc attgtagaag aaatattccc
     2101 aagcgatgca aattttttgc tggtaaaagt caaagatgca aacatgatct atcagtattt
     2161 gatcaaaaaa ggaatagtag tacggaatcg acacaacatg cctctttgta ccaactgctt
     2221 gcgtattact gtaggaacac ctgacgaaaa taatgttttg ataaataccc taaaaaaatt
     2281 atatatttaa cagaaattta aaatattaaa gagagttcat aaaaagtcac tttctttttt
     2341 gttgataagt ggttcgcaag ttggtgttag atgtggagaa taagataaag gcaaacaacg
     2401 ttactgttgt taaaagtgaa gccataattc gtgggcagaa tgttaatggt atcgaaatta
     2461 tttgtaatgg caaaaaatat tatagtgcca atttgttgat ttgcacgggc tcgcgaactg
     2521 ctatccctcc tatttgtaga ttgagtgaag ccggagatag tatggttact aatcatgaaa
     2581 tgctgaagtt gaaagcacaa cctccgtcgt cgttttctcg ctggaaatga aggtggtaag
     2641 gactttgtaa aatattgaca ggggaaatgg agaaatattt tcatatcttt gctgttgcct
     2701 cttgctagcg aaacaattta tggggcttgt atagccattg aactgaaaat gatgataaaa
     2761 gaacacagaa aatcgttccc ctcatttaac agtgagcgaa attatcaaac aaaccctttt
     2821 tacttttaaa aagattgtta aatcttacct gatttttaat tatatttgtt ctttatcaac
     2881 gaattaattt ctgaatgtaa tgctgaaaaa tagttaattt tttagacagg gttaaatttt
     2941 ttaaaaacac ttatttttaa aaggttgtaa ctctttttat aaaattgata ttataaaaat
     3001 aatctgctaa atttgttctt ttagcttaaa aaagctaact atgaaaaaca tttttatcta
     3061 ttttttcttc ttttttttga cgatttcttt gaacttcctt ttttgtccaa aaatttatgc
     3121 cgaatggaac agcttcataa tcaattatga taaaaatctt tatgggaaag gttctcaaac
     3181 atggcaaatt gttccttatg acacacgatg ggtatatttt gctaataaaa atggtttact
     3241 tgaattcaat ggaattttgt ggaatttatt cccgctcaat actggttcag atgctcgttc
     3301 ggtttatcct tcaaagagac aacaacgtat ttatgtagga ggcattaatg agtttggtta
     3361 ttttgaaccc aatgaaaaag gtaaattaaa ttacatctgt ctgtcagact catttgatat
     3421 tgctaattta caaattggaa atatctggaa aatttatgaa aatgacaaca tcctttattt
     3481 ccagggcgat aacaaaatca ttaaatatct gaatggaaaa ttttttccaa tagaagccca
     3541 tcgaaaaatc gagtgctcgg atttagttaa tggcgttatt tacattggca cagaagaagg
     3601 tgtatgggtt ttagttggca atactttttc ccctctgcaa ggtgcagaat ctttaatatc
     3661 taaaaggata cgaggtatca tgccatacgg aaaaggtata ttgattgtca ctgcttatga
     3721 tgggttatac tattatgatg aacatacgct taaaccttac ctcacaggtt ttgaaaattt
     3781 tatgaaacaa aatgaagtat tctgtgcagc ttcatcagaa caattaatag cattaggcac
     3841 tattcatgga ggtattctgt taatcaacaa tcaaaatcat acagtaaaat atttcaacga
     3901 aaataatgga ttacaga
//
LOCUS       DAOTKS010000122         3908 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_19587_length_3908_cov_5.050091, whole genome shotgun sequence.
ACCESSION   DAOTKS010000122 DAOTKS010000000
VERSION     DAOTKS010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19587_length_3908_cov_5.050091"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="P5236_03435"
     CDS             complement(<1..687)
                     /locus_tag="P5236_03435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRU63447.1"
                     /translation="MQKRYLNAFQYINEQAYTTEKYILPFIREIKEINTSTRVLEIGC
                     GEAGNLKPFLDAGCKCTGIDISSVKIEKGKEFFATHPLAENLTLICQDIYQINNELHD
                     FDIIILRDVIEHMPDKEKLLNLLKKWLAVDGIVFIAFPPWQNPFGGHQQICESKFLSV
                     LPYFHLLPVSLYKIILKFFGEKEGKINTLLEIKQTGISLENFEQLMKKNDFQILKKVL
                     YFINPNYEIKF"
     gene            complement(696..2228)
                     /gene="gltX"
                     /locus_tag="P5236_03440"
     CDS             complement(696..2228)
                     /gene="gltX"
                     /locus_tag="P5236_03440"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRU63448.1"
                     /translation="MNDNLTNVRVRFAPSPTGPLHIGGVRTALYNFLFARKNKGTMLL
                     RIEDTDSNRFVPGSEEYIIESLNWLGIEIDEGIGAKTEGKYGPYRQSERKEIYKQYVR
                     QLLNDGKAYLAFDSAIELAAKRAEIENFQYDAHTRNEMCNSLTLTPDEVKARIAKGEQ
                     YVVRIKIEPNEEITVNDLIRGEVTINSSVIDDKVIFKSVDELPTYHLANVVDDYLMQI
                     THVIRGEEWLPSSPLHVLLYRYLGWENEMPQFAHLPLLLKPDGKGKLSKRDGDRLGFP
                     VYPLNWVDPKTGEVYKGFREAGYFPEAVVNFLALLGWNPGTEQEIFTMEELIENFSLD
                     RVSKSGAKFDYEKAKWFNHKYLQMKSDEEIADTFQRMLVEKGFVEDSDKVLKIVSLIK
                     DRANFITDLWEQSHYFFEAPMQYDPKAVKKCWKPEIVPHLSELANFLENIKDFSAENL
                     EIEIKKWLAEKNYPLGNLMNALRLSLVGELKGPSIFEIVSILGKEETIRRIQKAIEEI
                     NT"
     gene            2531..>3908
                     /locus_tag="P5236_03445"
     CDS             2531..>3908
                     /locus_tag="P5236_03445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3836 domain-containing protein"
                     /protein_id="HRU63449.1"
                     /translation="MRTKITLFTMMLAIFSIGLFAQPDVWNRQEKIHSKFQKENNFQM
                     FSESYPSQEKEIPSLLNKRKQDQLKKDQLIQSERNVLPDFLKKKEGPLFIFSSRENKQ
                     NFTSKNFLQKAKNAYNVRAEIKQRLDSVVYVERDENTNEWLTSYKNEYAYDERGNLIL
                     EAWYHWDSDTERWEGDYKSEYIYDENRNCISEAGYDWDSDTEQWEGDCKYEYTYDERG
                     SLILKAYYRWDSDTEQWEVYYKYEYTYDERGSLILKAYYRWDSDTEQWEEYNKYEYTY
                     DERGNLILEAQYYWDSDTEQWGGDYKYEYAYDERGNLILEVDYNWNNINQQWQPTYKY
                     AVDYDYNYFNDDLLLPPYVDFKNKVVNYIVYKSSDGVNWTESGKGIYYYSDIEMAVSQ
                     ISMGSIKLYPNPVVNDLNIVLPDDIQQAEFKLFDLHGRMLVTKNIVADKKVNLEKLAA
                     GVYLYQIIT"
BASE COUNT         1336 a          635 c          635 g         1302 t
ORIGIN      
        1 aaatttaatt tcatagttgg gattaatgaa atataacact ttctttagaa tttgaaaatc
       61 attttttttc atcaattgct caaaattttc aagactaata cccgtttgtt tgatttccaa
      121 caaagtattg atttttcctt ctttttcacc aaaaaatttc aaaataatct tatataatga
      181 aacaggcaac aaatgaaaat aaggtaaaac agataaaaat ttactttcac aaatttgttg
      241 atggccgcca aaagggtttt gccacggcgg aaaagcaata aaaacaatgc catcaacagc
      301 caaccatttt tttaacaaat tgagaagttt ctctttgtca ggcatgtgct caataacatc
      361 tcgcaaaata atgatatcaa agtcgtgtag ctcgttgttt atctgataaa tatcttgaca
      421 gatcagggtc agattttctg ccaatggatg cgttgcaaaa aattctttac ctttttctat
      481 tttaacagaa ctaatgtcga tacctgtaca tttgcaacct gcatcaagaa aaggttttag
      541 attccctgct tccccacaac cgatttcaag gacacgagta gaggtattta tttcttttat
      601 ttctcgaatg aatggtaaaa tatatttttc agtagtatag gcttgttcgt tgatatattg
      661 aaaagcattc aaataacgtt tttgcattct tctttttacg tattaatttc ttcgatagct
      721 ttttgaattc gccttattgt ttcttctttt cccaatatcg acacaatttc aaaaatcgaa
      781 ggtcccttta attcacctac cagcgacaat cgaagagcat tcattaaatt tccaagcgga
      841 taattttttt ctgccagcca ttttttaatt tctatttcaa gattttcagc agaaaaatct
      901 ttgatatttt caagaaaatt agccagttca cttaaatgtg gaacaatttc tggtttccag
      961 cattttttta ccgcctttgg atcatattgc atcggagctt caaaaaaata atgagattgt
     1021 tcccataaat cagtaataaa atttgctcgg tctttgatta aagagacgat tttaagcact
     1081 ttgtctgaat cttcaacaaa tcctttttca accaacattc gttgaaaagt atctgctatt
     1141 tcttcgtccg atttcatttg caaatattta tgattgaacc atttggcttt ttcatagtcg
     1201 aattttgcac cgctcttgct gacccgatcc agcgaaaaat tttcgattaa ttcttccatt
     1261 gtaaaaattt cctgctctgt tccaggattc cagcccaata gagccaaaaa gttcaccact
     1321 gcttctggaa aatagccagc ttcgcgaaaa cctttataaa cttcacctgt ttttggatca
     1381 acccagttaa gtggataaac aggaaatccc aatctgtcgc catcgcgttt actgagttta
     1441 cctttgccat caggtttgag caaaagcggg agatgagcaa actgtggcat ttcgttttcc
     1501 caaccaagat accgatatag caacacatgc aaaggagacg acggcaacca ttcttcacca
     1561 cgaatgacat gagttatctg cataaggtaa tcatctacaa cgttagccaa atggtaagtg
     1621 ggcaattcat caaccgattt gaaaataact ttgtcgtcaa tcaccgaaga attgatggta
     1681 acttcaccac gaattaaatc atttaccgta atttcttcat ttggttcaat cttgatacga
     1741 acgacatatt gctcaccttt tgcaattctt gcttttactt cgtcaggcgt caatgtcaga
     1801 gaattgcaca tttcatttcg cgtgtgagca tcgtactgaa aattttctat ttctgcccgt
     1861 tttgcagcca gttcaatagc cgaatcgaaa gcaagataag ctttcccgtc atttaataat
     1921 tgcctgacat attgtttgta aatttctttt cgttcgcttt gtcgataagg accatatttt
     1981 ccttccgttt tagcaccaat gccttcatca atttctattc ccagccagtt tagtgattca
     2041 ataatatatt cttctgagcc aggaacgaat ctgtttgaat cagtgtcttc aattcttaac
     2101 aacatggttc ctttattctt tctggcaaaa aggaaattgt acaatgccgt ccgcacaccg
     2161 ccaatgtgta aaggtccggt tggacttggt gcaaatctta ctctcacgtt tgttaaatta
     2221 tcattcattt tttctattca aaaatttggt gactttgcaa agttaatgaa tttcacttat
     2281 aagaaagtga tttttaaaaa aaatgataag gaaaagaatt tatgtgaatg ttctttatga
     2341 aatttttagt ttttattcgc ctatagcaag ctttatctat cttaaaattt attttttata
     2401 cttacccaaa aactttttgt acaataaaga acaaatttgc aaaacatccc ttatatttgt
     2461 aaaaaataaa taacaaaaat tgacgaactt aaaaattcac taaaagttta actttaaaaa
     2521 acaaattatt atgagaacaa aaattactct ttttactatg atgctggcaa ttttttcgat
     2581 aggtttattt gcccagccag atgtatggaa tagacaggaa aagattcact caaaatttca
     2641 gaaagaaaat aattttcaaa tgtttagcga atcatatcca tcacaagaaa aagaaattcc
     2701 ttctttgctt aataaaagaa aacaagatca attaaagaaa gatcaattaa ttcagtccga
     2761 gcgtaatgtt cttccagatt ttttgaagaa aaaagaagga ccacttttta tattttcttc
     2821 cagggaaaat aagcaaaatt ttacttctaa aaattttcta caaaaagcta aaaatgctta
     2881 taatgtcaga gccgagataa agcaacgttt ggacagtgtt gtatatgtag aaagagatga
     2941 aaataccaat gaatggttaa ccagttataa aaatgaatat gcgtatgatg aaagaggaaa
     3001 tcttatatta gaagcatggt atcattggga ttcggatact gaacgatggg aaggagatta
     3061 caaaagtgaa tatatttatg atgaaaatag gaattgtata tcagaagcag ggtatgattg
     3121 ggattcggat actgaacaat gggaaggaga ttgcaaatat gaatatactt atgatgaaag
     3181 aggaagtctt atattaaaag catattatag atgggattcg gatactgaac aatgggaagt
     3241 atattacaaa tatgaatata cttatgatga aagaggaagt cttatattaa aagcatatta
     3301 tagatgggat tcggatactg aacaatggga agaatataac aaatatgaat atacttatga
     3361 tgaaagagga aatcttatat tagaagcaca gtattattgg gattcggata ctgaacaatg
     3421 gggaggagat tacaaatatg aatatgctta tgatgaaaga ggaaatctta tattagaagt
     3481 agattataat tggaataata ttaatcagca atggcagcca acctataaat atgcggttga
     3541 ttatgactat aattatttca atgatgatct tttattacca ccttatgttg atttcaagaa
     3601 taaggttgtt aattatattg tttataaaag tagcgatggt gtaaactgga cagaaagtgg
     3661 caaaggaatt tactattatt ccgatattga gatggcagtt tctcaaattt caatgggaag
     3721 cataaaactt tatcccaatc ccgtagtaaa tgatttgaac attgtattac ctgatgacat
     3781 tcagcaagca gaattcaaac tttttgattt gcatggaaga atgttggtaa ccaaaaatat
     3841 tgttgcagat aaaaaagtta atctcgaaaa acttgcggca ggtgtttatt tatatcaaat
     3901 catcactg
//
LOCUS       DAOTKS010000123         3893 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_19688_length_3893_cov_4.671443, whole genome shotgun sequence.
ACCESSION   DAOTKS010000123 DAOTKS010000000
VERSION     DAOTKS010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19688_length_3893_cov_4.671443"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1613)
                     /locus_tag="P5236_03450"
     CDS             complement(<1..1613)
                     /locus_tag="P5236_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63450.1"
                     /translation="MRIKHFLFLFFPLLLFVGKSEAQTTFSFAQKINWIGVQKWFADS
                     SYINVISFENACYPSDNFLPYFNYKQEMEPSFSYEASITNPVWIPVAPDELKIVEQYS
                     FVAEPEIKSNVAIEKGVSFFSVNILPFVEREGQYFKLLSFDLVIKKTSAPQKTKASTL
                     HTYATSSVLAQGKFVKIKVANNGIYKITYDDLVKMGINPTNVRVFGYGGGVLEQSFLK
                     NKIDDLPEVAIWMEKGKDGIFNTGDYILFYAQGPVKWYYDEAKSKFVHVNNPYSLYGY
                     YFITSDAGVGKKIEEKTIEVPSDAVIHQITEFTDYQVYEKDQVNLCSSGKEFYGEIFN
                     TRTSYEFSFNFPNILYTNTTKVTVDVAATSSANSSFTISLNNTQSKSLTVQKISGDHY
                     EMGKAATGSYIFTPNSETLNFKIDYNKSTSTSTGYLNYIEVNARRRLQMSGSVMQFQN
                     VDYLNKSSNYNNYILSNINSNIQIWDITDPQNITRVPFTMVNDNTLQFTVPGYELHTY
                     LAIDPAAGSSFSTPETVGKVVNQNLHGLQP"
     gene            complement(1696..2307)
                     /locus_tag="P5236_03455"
     CDS             complement(1696..2307)
                     /locus_tag="P5236_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRU63451.1"
                     /translation="MKIICIGENYPEHNKELNTTNPGEPVVFIKPDTAVLKNNKPFYI
                     PDFTNELHYETELIVKINRLGKNIAAKFASRYYNEIGLGVDFTARDLQRKLRAEGKPW
                     EICKAFDNAAVIGNFLPISYFKNLQDISFHLNINEKTVQRGNSKDMIFSIDELIAYVS
                     RFFTLKIGDILFTGTPAGVGKVSVGDHLEGYIFEEKMFDFFVK"
     gene            complement(2330..2962)
                     /locus_tag="P5236_03460"
     CDS             complement(2330..2962)
                     /locus_tag="P5236_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HRU63452.1"
                     /translation="MDFKVPEPTLRRLPWYLAYAKLMLEKGETFLSSTQIANDIAIDA
                     SIVAKDLSFVKISGRTRVGYDIKELIYVLERFLGFNVSHKAFLFGVGNLGGALMHDNG
                     LEQFGLKIVAGFDVKYELIGTSINKIPIHHIDHFPELQKQTGVIIGILTVPVDKAQKM
                     AELIVDGGIKAIWNFTPFRIRVPENVVVQNTSIYAHLAVMFNRLNAILGK"
BASE COUNT         1364 a          637 c          606 g         1286 t
ORIGIN      
        1 ggctggagcc catgcaaatt ctggtttaca accttaccca ctgtttcggg cgtactaaaa
       61 gaagaacctg cagctggatc aatagccaaa taggtatgta attcgtaacc tggaacagtg
      121 aattgtaaag tattatcatt caccatagta aatggtactc gtgtaatatt ttgtggatca
      181 gtaatatccc atatttgaat attactgtta atattactga gaatataatt attatagttg
      241 gaggatttat ttagataatc tacattttga aattgcatta cagagccact catttgtaat
      301 cttcgacgcg cattgacttc aatataattt aagtaacctg ttgatgtaga agtagattta
      361 ttataatcaa ttttaaaatt taaagtttca ctattgggag tgaaaatata agatcctgta
      421 gcagccttgc ccatttcgta atgatctcct gatatttttt gaacagtcaa agatttcgat
      481 tgagtattat ttaaactaat tgtaaaggag gaatttgctg acgaagttgc agcaacgtcc
      541 accgtaactt ttgtggtatt ggtatataaa atattaggga aattaaaaga aaattcataa
      601 ctagttcgcg tattaaaaat ttctccataa aattcttttc cagaactaca caaattaact
      661 tgatcctttt cgtaaacctg ataatcagta aattctgtaa tctgatgaat cacagcgtct
      721 gaaggaactt caattgtttt ttcttctatt tttttgccaa ctcctgcatc agaagtaata
      781 aaataatatc catataggga ataaggatta tttacatgta caaattttga ttttgcctca
      841 tcataatacc atttcacagg tccttgtgca tagaataaaa tataatcacc ggtgttgaaa
      901 atgccatcct ttcctttttc catccagatg gctacttcag gcaaatcatc aatcttattt
      961 ttgagaaaac tttgttccaa aacaccacct ccataaccaa aaactcgaac gttggtagga
     1021 tttattccca ttttaaccaa atcgtcatac gtaattttat aaataccatt attagcaact
     1081 ttaattttta caaatttccc ttgagccaat accgaagaag ttgcataagt gtgaagagtg
     1141 gatgccttag ttttttgcgg agcacttgtt tttttgatca ccagatcgaa acttaacaat
     1201 ttaaaatact gaccttcccg ttcaacaaaa ggcaatatat ttacgctaaa aaaagaaact
     1261 cctttttcaa tagctacatt cgattttatt tctggctcag caacaaaaga atattgttct
     1321 acgattttta gttcatccgg cgccacagga atccatactg gatttgttat ggaagcttcg
     1381 taggaaaagg aaggttccat ttcctgttta tagttaaaat atggcagaaa attatcagaa
     1441 ggatagcaag cattttcaaa agaaataaca ttgatatagc ttgaatcagc aaaccatttt
     1501 tgaacaccta tccagttgat tttttgagca aatgaaaaag ttgtttgagc ttcacttttt
     1561 ccaacaaaaa gtagcaaggg aaaaaacaga aacagaaaat gttttattct cataaaatga
     1621 attacctcac aatacaaagt taacagatat gaaacgctaa aaaaaattat ttattatttg
     1681 aactgaattt tgaaattatt taacaaaaaa atcaaacatt ttttcctcaa aaatataacc
     1741 ttccaaatgg tctcctacag aaactttccc cactcccgca ggggtacccg taaataaaat
     1801 atccccaatt tttaaggtaa aaaaccgact cacataagca atcaattcat caatggaaaa
     1861 aatcatatct tttgaattgc ctctttgaac agttttctca tttatattca aatgaaaaga
     1921 aatatcctgt aaatttttaa aataactgat aggcaaaaaa ttaccaataa cagccgcatt
     1981 atcaaaagcc ttgcaaattt cccagggttt tccttcggct cttaattttc gttgtaaatc
     2041 acgagcagta aaatctactc ctaacccaat ttcattgtaa taacgagagg caaatttcgc
     2101 cgcaatattt ttacctaaac gattgatttt tacaatcaat tccgtttcat aatgcaactc
     2161 gttggtaaaa tctggaatat aaaaaggctt gttgtttttc agcactgctg tatcaggttt
     2221 tatgaaaaca actggctctc ctggattagt agtattaagc tccttattgt gttcaggata
     2281 attttctcca atacaaatta ttttcatttt aatttcttat ctctaaattt tatttcccca
     2341 aaatggcatt caatctgtta aacatgaccg ccaaatgagc ataaatagaa gtattttgta
     2401 ccaccacatt ttcaggaacc cgtatgcgaa aaggagtaaa attccagata gcttttatgc
     2461 cgccatcaac tattaattca gccatttttt gagccttatc gacaggaacg gttaaaattc
     2521 caataatcac tcctgtttgt ttttgcaatt ctggaaaatg atcaatatga tgaataggaa
     2581 ttttattaat gctcgtgcct attaattcat attttacatc aaaacctgca acaattttta
     2641 aaccaaattg ttctaaacca ttatcatgca tcaaggctcc acccaaattc cctactccaa
     2701 acaggaaagc tttatgagaa acattaaatc ctaaaaaacg ttccagcaca taaataagtt
     2761 ccttgatatc ataacctaca cgggtacgac ctgaaatctt gacaaatgac aaatcctttg
     2821 ccacaatgga agcgtcaata gcaatatcat tggcaatttg cgtagaagat aaaaaagttt
     2881 cccctttttc aagcatcaac tttgcatagg ctaaatacca cggcaaacga cgcaaagtag
     2941 gctcagggac tttaaaatcc atttctggtt tgttattgtt cacttcaatg gcattataaa
     3001 catttatctc acaaaaataa tcattttttt taaatttttc tttttaagtc attttcttat
     3061 acttatatat gttgtatttt tagttaaata attgaaaatc aacaaattta ttttaaattt
     3121 atttatctaa tttcctgaaa accatcgctt tataaatcaa gtcacaattt tttaagaaat
     3181 aagcagatgc cgaaaaaatc agtttgttta tctcataggt aatatatttt gatagcgatt
     3241 tggtatactg aggtttttgg aaaaatattg aaatggattg gcaacgcaaa caacaaaatt
     3301 tagaaatgct tttaggagta tacttttgaa aagtaatcaa atttaaaaat ataatgtttg
     3361 aaaatcagag aaaaaaatcc tgaaattgaa atagatttca acgaaaataa attagagagt
     3421 gtaaatattt taagagactt taaggcgttg aagttgacgg aaagttttca gaattcaagc
     3481 atttgaaaag aagtggttac agttttcaat tggtattgtc aacaatgatc tgtgcagtga
     3541 cattggggag taacacatca gtatcttttt tgcctctatt aagggataaa gggagcatga
     3601 tgggtaagga ggtattgtat cgtttgaaga atataatcgg gggatcaatt ggcagaagat
     3661 attatggcaa attgcttgta agtttatcgg gataacggca gaggagagtg aaggagaata
     3721 taacgatgcc tcatatttga tgataccttg cttgaaaaga taggtaaaaa gatagaaaag
     3781 attgggaagg tgcatgatca tgtaacaaat gctttcaaat tgagatccaa gttattattg
     3841 ggattgtatt gggaatggaa agtcaatgat acgttggatt ttacaattgt tga
//
LOCUS       DAOTKS010000124         3868 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_19841_length_3868_cov_2.882769, whole genome shotgun sequence.
ACCESSION   DAOTKS010000124 DAOTKS010000000
VERSION     DAOTKS010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19841_length_3868_cov_2.882769"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="P5236_03465"
     CDS             <1..905
                     /locus_tag="P5236_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRU63453.1"
                     /translation="PVNKDGEVFKDGAFCEIILDKTAAKGNARERLFGPPTSGGGGEG
                     GYIYKELADAFPMIDGKPIGQSKYTYDVFNPNVNRDPRFANSVLYDGGYFCTGVSAYT
                     PIRISQGVGATLDAVHVGTPTGFYVKKTVSRATCGNSFIGVPQSRPLIRYAEILLNYA
                     EAANEYYGPDYNDQYTSVYDALKAIRARGGIEPGDDGMYGLKPNMTQDEMREAIRLER
                     RIELAFEGHRFFDVRRWKIAEETDNKVMHGLEITVNNDGSKSWQEFEVAKHTFRPAMY
                     FWPIPYDEVTKSNDLLQNPFYSAE"
     gene            932..1384
                     /locus_tag="P5236_03470"
     CDS             932..1384
                     /locus_tag="P5236_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5004 domain-containing protein"
                     /protein_id="HRU63454.1"
                     /translation="MKSKILKTVTLLSFFTFLISCDAFTDSEFNDYKESVKDLSGVWQ
                     LKTVSRNGIDISSTMNFSQFHLNLNPNGSYTLENYLPFAVKDNGEWRVDDPQYPYNLI
                     LRENNSSEDITIGLKYPIVNGKRIISITLSPGCQSNTYVYVFEKIDNN"
     gene            1398..2471
                     /locus_tag="P5236_03475"
     CDS             1398..2471
                     /locus_tag="P5236_03475"
                     /inference="COORDINATES: protein motif:HMM:NF027652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4961 domain-containing protein"
                     /protein_id="HRU63455.1"
                     /translation="MKNIFKNKKNTRLIGLFLTVGFVLFLIGCVFIDSVSILQIQEDG
                     TQAPRTKAGSVATFILTGHINCQEDHGDVQFIFSFLAPKSWNVRQNATVTYTTTLATN
                     PEEELKMSVVPESSLPKNGGGRTWGEALMQEYGVGPNVLSDMEWVTFATNDKWAIYNG
                     DKPNYTIYLRTNVGYQNLKAYLGFFVNHTDDGMSTSADHKKVKFSDTPFEVYGGKGLM
                     IDYSTDHFNKVQPLASLQDDYVTFSFNGGVYQNDLISCSEIYFEGIAYDENGEIISEV
                     NEKTDKTLLHRESMFSQVYSLTMWPASFFNVPQGKEIKKIDYVFTNKDRSIIITQSDD
                     DFAINHIPINSEKEPFVFELLCD"
     gene            2516..>3868
                     /locus_tag="P5236_03480"
     CDS             2516..>3868
                     /locus_tag="P5236_03480"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRU63456.1"
                     /translation="MRNNKCQYLILFMLIWLVMGIQSVCVKAQDNSSKSSLTNIVLDP
                     YGNPVSNVEIGIKGSDIKTFTKINGTFDLDYKVGDILTYSHKNFMYSESKVSKKDVKE
                     GIQIHLQEKSIKNPSNVPEPYGEIIDKNSYLGSASTIYTDQLTKTMGSTVIPAMVGRI
                     SGLNIIQTRGARLHYTSANYNADLAGLIPVFGRRVYSDNSEFSLNARGQAPIVIVDGI
                     QREFYSLDPEAIESISLQKDALSSMFLGMQSSRGALIITTKKPTQGAMNISFTGRYGI
                     TTPLQLPKPLNSYQYTYLLNEALTNDGKLPIYSYEDFEKYRSGNSPYTHPDVNWFDEL
                     LNNNASTQSYNLNVSGGNNVAQYYVSLGYMNEDGLFITSSKNPYNTNLNYQRYLITSK
                     INVKVTRDFTTNIMLVGRIEDGNQPGGNGSGYADLLNTIFTTPNSAYPITNPDGSWGG
                     N"
BASE COUNT         1346 a          546 c          718 g         1258 t
ORIGIN      
        1 taccggtgaa taaagacgga gaggtattta aagatggtgc tttttgtgaa attattttag
       61 ataaaactgc ggcaaaaggt aatgcacgtg aaaggttatt tggaccacct acaagtggag
      121 gaggaggcga aggtggttat atatataaag aattagcaga tgctttccca atgatcgatg
      181 gtaaaccgat tggacaaagt aaatatacct atgatgtatt caatcccaat gtaaatagag
      241 atcctcgatt tgcaaattct gttctttatg atggtggtta tttttgtaca ggggtatccg
      301 cttatactcc aatcagaatt tcgcaaggcg ttggcgctac cttagatgct gttcacgttg
      361 gaactcctac tggtttttac gtaaaaaaaa ctgtgagtcg tgctacctgt ggtaattcct
      421 ttatcggtgt accgcaaagt cggccattaa ttcgttatgc tgaaatttta ttaaattacg
      481 ctgaagcagc taatgaatat tatggaccag attataatga tcaatatact agtgtttatg
      541 atgcgttgaa agctattcgc gcaagaggtg gaatagaacc aggggatgac ggtatgtatg
      601 gtttaaaacc aaatatgaca caagatgaaa tgagagaagc tattagatta gaaagaagaa
      661 tcgaattggc tttcgaaggt catcgttttt ttgacgtacg tcgttggaaa attgccgaag
      721 aaactgataa caaagtaatg catggcttag aaattaccgt taataatgat ggttcaaaat
      781 catggcagga atttgaagta gcaaaacaca cattccgccc tgctatgtat ttttggccta
      841 taccatacga tgaagttaca aaatcaaatg accttttaca gaatcctttt tattcagctg
      901 aatagtgtta attaataata taaataacca tatgaaatct aaaatattaa aaactgttac
      961 cttactttct ttttttactt ttttaatctc atgcgacgca tttactgata gtgaatttaa
     1021 tgattacaaa gaaagtgtta aagatctaag tggggtatgg caattaaaaa ctgtgtcacg
     1081 aaatggtatc gatatttcta gcacaatgaa ttttagccaa tttcacttga atttaaatcc
     1141 gaatggaagc tatactttag agaattattt accttttgct gttaaagata atggggaatg
     1201 gagggttgat gatcctcaat atccttacaa tctaatttta cgcgaaaaca attcaagtga
     1261 ggatataact attggattaa aatatccaat agtaaatggg aaacgcatta tctccataac
     1321 tttaagtcct ggatgtcaaa gcaatactta cgtttatgtg tttgaaaaaa ttgataataa
     1381 ttaaatataa ggattaaatg aagaatatat ttaaaaataa gaaaaatact cgattgatag
     1441 gattatttct aacagtagga tttgtactgt ttttaattgg atgtgttttc attgatagtg
     1501 tttcaatttt gcaaattcaa gaagatggta cacaagcacc tagaacaaaa gcaggttctg
     1561 tggctacatt tattctaaca ggtcatatta actgtcaaga agatcatggg gacgtacaat
     1621 ttattttttc tttcttagca cctaaatcat ggaatgttag gcaaaatgct acagttacct
     1681 atactactac tttggctaca aatcctgaag aagaacttaa aatgtcagtt gttcctgaat
     1741 catcattacc caaaaatgga ggaggacgta cctggggaga agctttaatg caggaatatg
     1801 gagtaggtcc taacgtttta tctgatatgg aatgggtaac ttttgcaaca aatgataaat
     1861 gggcaatata taatggagat aaaccaaatt ataccattta tttacggact aatgtaggat
     1921 atcaaaattt gaaagcttat ttaggttttt ttgttaatca tactgatgat gggatgagta
     1981 ctagtgccga tcataaaaaa gtgaaattta gtgatactcc ttttgaggtt tatggaggca
     2041 aaggcttgat gattgattat agtactgatc actttaataa agttcaacca cttgcttctt
     2101 tacaagatga ttatgtgact tttagtttca atggaggtgt ttatcaaaat gacttaattt
     2161 catgtagtga aatttatttt gagggaatag cttatgatga aaatggagag ataatatctg
     2221 aagtgaatga aaaaactgac aaaaccttac ttcacagaga gtctatgttc agtcaggttt
     2281 atagtttgac tatgtggcct gctagttttt tcaatgttcc tcaagggaaa gagattaaaa
     2341 aaattgatta cgttttcacc aataaagata gatctattat tataactcaa tctgatgatg
     2401 attttgcaat aaatcatata ccaataaata gtgaaaaaga accttttgtg tttgaactat
     2461 tatgtgatta atattaactg aataaataaa tagataaaaa ttaaaattaa tttctatgag
     2521 aaataataaa tgccaatatt taatattatt catgctaatt tggttggtaa tgggaattca
     2581 atcagtatgc gtgaaagcac aagataatag ctcaaaatct tccctcacaa atatagtgct
     2641 cgatccttat gggaatcctg tctcaaacgt tgagattgga attaaaggat ctgatatcaa
     2701 aacatttact aaaatcaatg gaactttcga tttggattat aaagtaggtg atatattgac
     2761 atatagtcat aaaaatttta tgtattcaga atccaaagtt tctaaaaaag atgtgaagga
     2821 aggtatacag atacatttgc aagaaaagtc aataaaaaat ccttcaaatg ttccagaacc
     2881 ttatggagaa attattgata aaaatagtta tttagggtct gcatcaacta tttatacgga
     2941 tcaactaaca aaaacaatgg gtagcactgt tatacctgct atggttggcc gtatttctgg
     3001 cctgaacatt attcagacca gaggtgcccg tctccattat acttcggcaa actataatgc
     3061 cgatcttgca ggattaatac ctgtatttgg tagaagagtt tacagtgaca attctgaatt
     3121 ttctttaaac gcgagaggac aagctcccat tgtaattgtt gatggaattc aaagagaatt
     3181 ttattcatta gatcctgagg cgattgagtc aatttcctta caaaaagatg ccctgtcctc
     3241 aatgtttctt ggtatgcaaa gttcacgtgg agctctaata attacaacga aaaaaccaac
     3301 acaaggtgct atgaatatat cttttacagg tagatacggt attactactc ctttgcaact
     3361 tcctaagcca ttaaattcat atcaatacac ttatttgtta aatgaagcac taactaatga
     3421 tggtaaatta ccgatttata gttatgaaga ttttgaaaaa tatagaagtg gtaatagccc
     3481 ttatactcat ccagatgtaa attggtttga tgaattatta aataataacg catcaacaca
     3541 atcctataat ttgaatgttt cggggggaaa taatgtggct caatattatg ttagcttagg
     3601 ttatatgaat gaagatgggt tgtttataac ttcttctaaa aatccatata acacgaattt
     3661 gaattatcag cgatatttaa ttacttcaaa gataaatgta aaagtaacgc gtgattttac
     3721 tacaaatata atgttggttg gaagaattga agatggcaat caaccgggtg gcaatgggag
     3781 tggctacgct gatttactta ataccatatt tactactcca aattctgctt atcctattac
     3841 caatcctgat ggttcgtggg gtggaaat
//
LOCUS       DAOTKS010000125         3857 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_19911_length_3857_cov_3.639926, whole genome shotgun sequence.
ACCESSION   DAOTKS010000125 DAOTKS010000000
VERSION     DAOTKS010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19911_length_3857_cov_3.639926"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1529)
                     /locus_tag="P5236_03485"
     CDS             complement(<1..1529)
                     /locus_tag="P5236_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63457.1"
                     /translation="MAIHPCVRNFSHKTTHSGTQNWDIIQYRNNWMYFANNNGLLEFD
                     GNKWTVYPISNYTNVRSLYYDIKTDRIYAGAFNEFGYYSRDEAGLLHYYSLIEKIPAK
                     NRNFNEIWNISAVDNSIYFQGDKDIFQYKNDQISHFSFPAKIDCSAVVFNSLIVCSKQ
                     NGTFFLNGDMFIKIPNSELLTNKRVSAILPFQKNNVLFVTEFNGLFTFDGNKIEPFKT
                     DIDQFMSENQVFCAAIKDYKLAIGTVRKGLVIKDFQENSNFFSNTFSGLQNNTILSLA
                     FDNQDNLWLGLDKGIDYVLINSPIYELISSINQIGTGYCSLIENENLYLGTNQGLYCT
                     LYPIPVSPDPPPIQLINGMQGQVWCLMKIDGTIFCGTDHGAYYFSGNKIVQIPGIPGT
                     WSFRKMNQHPDCVLGSSYQGFFILKKNKQKWEFSNFIKGFTDSGGMFEMDENDNIWFS
                     HWIKGISKLTLNQTLDSVIHIETYDTTKGLPTNRNNTLLLYKNSIKFSSEHGFYKYNK
                     K"
     gene            1938..2108
                     /locus_tag="P5236_03490"
     CDS             1938..2108
                     /locus_tag="P5236_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63458.1"
                     /translation="MKLVTGKNLCFCGRWFVGGLAEWGVAPNLKLQILDYHVNIQNTE
                     LETYTSYLDKHF"
     gene            complement(2154..>3857)
                     /locus_tag="P5236_03495"
     CDS             complement(2154..>3857)
                     /locus_tag="P5236_03495"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HRU63459.1"
                     /translation="KRIKTLYEQKDKLGLSFEQNRLLEKYYKNFVRNGAALNAEQKAQ
                     LREINKELSLAELQFGQNVLAETNAYQKWITDEKDLAGLPDNVRQAAAEAAEQAGRKG
                     AWLFTLQKASFIPVLQYSKNRDLRKELVTAYTHIANNNNQHDNKAVINKIMKLRIQKA
                     QLLGFPTSADYYLDDTMAKTPDKVYSFLASLWEPSLKKAKEEAAELQQLMDTEGKGEK
                     LEPWDWWFYAEKLRKIKYDLNEEVLRPYFKLENVRQGVFDLASRLYGLQFKKLNNMPV
                     YHPEVEVFEVLDADGSLVGILYTDYFPRAGKQSGAWMNNISEQYIKNGVNFRPVICNV
                     GNLTKSIAEHPSLLNMDEVETIFHEFGHALHGLLAQSTYPSLSGTNVVRDFVEFPSQI
                     MENWCWEPEVLKTYARHYQTNEIIPDDLIEKIQQSRLFNQGFNMTELLAAAYLDMDYH
                     TRKDTSDFNVEDFENASMAKIGMIPQIHVRYHSTYFNHIFTGGYCAGYYSYSWAEVLA
                     DDAFQAFKETGDVYNQELSFAFRKKILERGDSDEPMKLYIDFRGKEPLPDALLIKRGL
                     K"
BASE COUNT         1241 a          682 c          613 g         1321 t
ORIGIN      
        1 tttttcttgt tatatttata aaatccatgc tcgcttgaaa atttaattga atttttatac
       61 aataataagg tgttattcct attggtaggc aaaccttttg tcgtatcata agtttcaata
      121 tgtatgacgc tatctaaagt ttgattcaac gttagttttg aaataccctt gatccagtga
      181 ctgaaccata tgttgtcatt ttcgtccatt tcaaacattc ctcctgaatc cgtaaaacct
      241 ttaatgaaat ttgaaaattc ccatttttgt ttgttctttt tgagaataaa aaaaccttga
      301 taagaagatc caagtacgca atcaggatgt tggttcattt ttcggaaact ccaagtacct
      361 ggtatccctg gaatctgtac aattttgttg cctgaaaaat aataagctcc gtggtctgtt
      421 ccacaaaaga tggtcccatc gattttcatt aaacaccata cttgcccttg cataccattt
      481 atcagttgaa taggaggtgg atctggcgag acaggtattg gatataaagt gcagtacaag
      541 ccttgattag tgccaaggta taaattttca ttttctatca gagaacaata accagtaccg
      601 atttgattaa tactcgaaat taattcgtaa atgggagaat taataagaac ataatcaatg
      661 cctttatcaa gtccaagcca taaattatct tgattgtcaa acgctaaact taaaatggta
      721 ttattttgca atccactgaa agtatttgaa aaaaaattgg aattctcttg aaaatcttta
      781 ataactaatc cctttcttac ggtaccgatt gccagcttat aatcctttat tgcagcacaa
      841 aaaacttgat tttccgacat gaattggtca atgtctgttt tgaaaggttc aattttgttc
      901 ccatcaaaag taaataatcc attgaattca gttacaaata ggacattgtt tttctggaaa
      961 ggtaaaatag cactgactct tttgttagtt aaaagttcag aattaggaat ttttataaac
     1021 atatcgccat ttaagaaaaa tgttccattt tgtttactgc aaacaatcaa agaattaaaa
     1081 acgactgctg aacaatctat tttagcaggg aaagagaaat gtgaaatttg atcattttta
     1141 tattgaaaaa tatccttatc tccttgaaaa taaattgaat tatcaacggc tgaaatattc
     1201 cagatttcgt taaagtttct gtttttagct ggaatttttt ctatcaatga ataataatgc
     1261 aatagtcctg cttcatcacg tgaatagtaa ccaaattcat tgaaagctcc ggcatatatt
     1321 ctgtcagttt ttatatcata ataaagagaa cgaacattcg tgtaattgct tataggataa
     1381 acggtccatt tatttccatc gaattccaac aatccgttgt tgttggcaaa atacatccag
     1441 ttgtttcgat attgaataat atcccagttt tgagtaccag aatgagttgt cttgtgcgaa
     1501 aaattccgga cacagggatg aattgccatt aaataccatt gaggaaaaat cagaaaagaa
     1561 attataataa aaaacttgtt gtttaataac tgttttagac aacaacagca atagattttc
     1621 atatcgaaac aaattggaaa ttatacatga aagattaaat taataggata gaagaatgat
     1681 atctttttat ttctatgtca ttaattttat gttccatttt aacctgagaa aatatttttt
     1741 atagtaaaaa aattaagtat ttacaagtca aatttaatat tttttttgaa aaaaaaattg
     1801 gaagttgatt ttttattata aaaagtactg aaagattctt ctttcttctt aattttttaa
     1861 agttagtttt ttcttactat tgtctatgta aacacctaaa aacgttgatt gtttttcaaa
     1921 gatttttact tgcaaatttg aaactcgtaa ctggaaaaaa tctttgtttt tgtggaagat
     1981 ggtttgtggg tgggttagct gaatggggcg ttgctccaaa cctaaaacta caaattcttg
     2041 attaccatgt aaatattcaa aacacagaac tcgaaaccta tacctcgtac ctcgacaaac
     2101 acttttgaaa agcaatttgg tatgaaacac aaaacaaatt ttggaataat gaactatttc
     2161 aatcctcttt taattaaaag tgcatcaggt agaggttctt tgccacgaaa atcaatatat
     2221 aatttcattg gttcatccga atcgccacgt tccagtatct ttttcctgaa agcgaatgat
     2281 aactcttgat tgtaaacatc acctgtttcc ttaaaagctt gaaaagcatc atcagccagc
     2341 acctctgccc aggaatagct gtaatagcca gcacaataac caccagtaaa aatgtgatta
     2401 aaataagtgc tgtgatatct gacatgaatt tgcggaatca taccaatttt tgccattgaa
     2461 gcattttcaa aatcttcaac attaaaatca ctggtatctt ttcgagtatg ataatccata
     2521 tccagataag cagcagccag caattccgtc atattaaatc cttgattgaa caatcgtgat
     2581 tgctgaattt tttcaatcaa atcatcggga attatttcgt tggtttgata gtgacgagcg
     2641 tacgttttca atacttcagg ttcccagcac cagttttcca tgatttgtga aggaaattct
     2701 acgaaatcac ggactacatt ggttcctgat aaactgggat aagtgctttg tgccagcaat
     2761 ccatgcaatg catgaccaaa ttcgtgaaaa attgtttcca cttcgtccat attgagcaag
     2821 gaaggatgtt ctgctataga tttggttaga tttcccacat tgcaaatcac tggtcggaaa
     2881 ttgacgccat ttttgatata ctgctcggag atattattca tccaggctcc cgattgtttg
     2941 cccgcacgag gaaaataatc agtatataaa attcccacca aagaaccatc tgcatccagc
     3001 acttcgaaca cttcaacttc tggatgataa acaggcatgt tatttaattt tttaaattgc
     3061 aagccataca atcgtgaagc cagatcaaaa acaccttgac gtacattttc cagtttaaaa
     3121 taaggtcgca aaacttcttc gttcagatca tacttaattt tgcgaagctt ttctgcgtaa
     3181 aaccaccaat cccacggttc cagtttttct ccttttcctt cagtatccat cagttgttgc
     3241 aattcagcgg cttcttcttt tgcttttttc aatgaaggtt cccaaagcga agccaggaaa
     3301 ctgtaaactt tatcgggcgt ttttgccatt gtgtcgtcca gataataatc agctgaagtt
     3361 gggaagccca gtaattgagc tttctggatg cgaagtttca taatcttgtt aataactgct
     3421 ttattatcat gctgattatt attgttggca atatgggtat aagctgttac caattctttt
     3481 cgtaaatcac gatttttact gtactgtaga acgggaataa aacttgcttt ctgaagagta
     3541 aaaagccagg cacctttcct accagcttgt tctgcagctt cagctgccgc ctgacgaaca
     3601 ttgtctggca aacctgctaa atctttttca tcggtaatcc atttttgata ggcattggtt
     3661 tctgctaata cattttgacc gaattgcaat tcagcgagcg ataattcttt gttaatctcc
     3721 cgaagttgag ctttttgttc cgcatttaaa gcagctccat tccgaacaaa attcttgtaa
     3781 tatttttcca gcaaacgatt ttgttcaaaa ctcaaaccca acttgtcctt ttgttcataa
     3841 agcgttttaa ttctttt
//
LOCUS       DAOTKS010000126         3855 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_19927_length_3855_cov_3.542632, whole genome shotgun sequence.
ACCESSION   DAOTKS010000126 DAOTKS010000000
VERSION     DAOTKS010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19927_length_3855_cov_3.542632"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..1410
                     /locus_tag="P5236_03500"
     CDS             175..1410
                     /locus_tag="P5236_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRU63460.1"
                     /translation="MVKILFFLIIFFVVADFVLERYLTYLNIQYSKQELPPILQNIYD
                     EEKYKKQQEYFRTNTRFSMLTSTLSFVIILLMLIFGGFGWLDNLLRKITEHQILLPLL
                     YFGVLFFASEIINLPFEWYDTFVIEEKFGFNKVTPKLFIVDKIKEILLSVIIGGGILA
                     TIIWIYTLTPNYFWLWAWLVVTFFSLIMNMFYSEIIVPLFNKQTPLPEGELRTEIEKF
                     ASKAGFKLSNIYVIDSSKRSTKANAYFSGLGSKKRIVLFDTLLNKLTTEEIVAVLAHE
                     IGHYKHRHTLYQFLISLPVNLLLFFLLGYILKSDIFAQVLGVASASFHINVIVFSLLY
                     APLSLLLGIFSNILSRKFEFQADAFAAQYGYATFLQSGLKKLSETSLSNLMPHPLYVF
                     FHYSHPTLYQRLTKLVSYT"
     gene            1422..>1774
                     /locus_tag="P5236_03505"
     CDS             1422..>1774
                     /locus_tag="P5236_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HRU63461.1"
                     /translation="MENNIFFLCPTAGVLALLFAWYFSHQMMKASEGTVTMKTIAKHV
                     REGAFAYLKQQYKVVAIVFLCLTILFAIMAYGFNIQNPWVPFAFLTGGFFSGLAGYIG
                     MRTATYASARAANAA"
     assembly_gap    1775..1874
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1875..3802
                     /locus_tag="P5236_03510"
     CDS             <1875..3802
                     /locus_tag="P5236_03510"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRU63462.1"
                     /translation="IGMRTATYASARAANAAQHSLNSGLKIAFRSGAVMGLVVVGLGL
                     LDISIWFYVLQFFIGALDNTTDLAIAADPSMKLIIITTTTLTFGMGASTQSLFARVGG
                     GIFTKAADVGADLVGKVEANIPEDDPRNPATIADNVGDNVGDVAGMGADLYESYCGSI
                     LSTAALGATAFAGFGTTTQFNGVIAPMIIASVGIILSILGIFLVRSKEDASQKQLLNA
                     LNRGIYTTAVLIAIFSFFILKYLNIPNSLGVWGSMLAGLLGGVAIGKITEYFTSAAYQ
                     PTRRISEASKTGPATVIISGIGTGMISTALPVIVVAISIILAYYFASGLDFTNISMGL
                     YGIAIAAVGMLSTLGITLASDAYGPIADNAGGNAEMSGLGPEARKRTDALDALGNTTA
                     ATGKGFAIGSAALTALALMASYLEETKVAIGRIPEKAQLFFDTVNTNIHQASIPQLME
                     FFQVNLMNPRVLVGAFIGAMAAFLFCGLTMNAVGRAAQSMVEEVRRQFREIVGIMEGK
                     AEPDYARCVAISTKGAQKEMVFPSLLAILIPIITGIFFGVPGVMGLLLGSVSTGFVLA
                     IFMANTGGAWDNAKKYIEEGNFGGKGSENHKAAIVGDTVGDPFKDTSGPSLNILIKLM
                     SMVSIVMAGLTVTWSLM"
BASE COUNT         1118 a          569 c          692 g         1376 t
ORIGIN      
        1 aacatttctc tcaaaaattc caaaaagtga ctttattaca aattattgaa caaaaaactt
       61 tttaaaacat agacgaatga tagcttattt tttaaaattc ataataaagg atagtttgaa
      121 tttattaaaa tcattatctt tgtggtcaat ttttagatag catcattcgt tatgatagta
      181 aaaatattgt ttttccttat tatttttttt gtcgtagctg actttgtttt agaacgttac
      241 ttaacctatc ttaatattca atattccaag caagaacttc ctcccattct tcaaaatatt
      301 tatgatgaag aaaaatataa aaagcaacag gaatattttc gcaccaatac ccgttttagc
      361 atgttaacaa gcacattaag ttttgttatc attctactga tgcttatttt tggtggcttc
      421 ggctggttag ataatctttt aagaaaaata acagaacatc aaattttatt acctctttta
      481 tattttggag ttttattttt tgccagtgaa ataattaatc ttcctttcga atggtatgat
      541 acatttgtga ttgaagaaaa atttgggttc aataaagtaa caccaaaatt atttattgtt
      601 gataaaataa aggaaatttt gctttctgtt ataattggtg gcggtatttt agccactatt
      661 atttggattt acacattaac acccaattat ttttggttat gggcgtggtt ggtggtaaca
      721 ttttttagtt taattatgaa tatgttttat tcagaaatta ttgtgccctt atttaataaa
      781 caaacacctt tgccagaagg agaattaaga actgaaattg aaaaatttgc ctcaaaagca
      841 ggttttaagc tatctaatat ttatgttatt gacagttcga aacgaagcac aaaagctaat
      901 gcttatttca gtggattagg aagtaagaaa aggattgtat tatttgatac tcttttaaat
      961 aaattaacca cagaagaaat tgttgctgtt cttgctcacg aaattggtca ttacaaacac
     1021 agacacactc tttatcaatt tttaatatca ttacctgtaa atttgttgtt gttttttttg
     1081 ctcggttata ttcttaaaag tgatattttt gcacaagtct tgggagttgc atctgcttct
     1141 tttcacatca atgtgattgt gtttagcttg ttatatgctc ctctttcatt gttgttggga
     1201 attttttcca atattttatc aagaaaattt gaatttcagg cagatgcttt tgcagctcaa
     1261 tatggttatg ctactttttt acaatcagga ttaaaaaagt tatctgaaac ttcattaagt
     1321 aatttaatgc cacatccatt atatgtgttt tttcattatt ctcatccaac tttatatcaa
     1381 cgtttaacta aattagtttc ttatacataa attaaattct tatggaaaat aacattttct
     1441 ttttgtgtcc aacagcagga gttttagcac tactatttgc ttggtatttt tctcatcaaa
     1501 tgatgaaagc aagcgaagga actgtaacaa tgaaaaccat tgctaaacat gtacgagaag
     1561 gggcttttgc ctacttaaag cagcaatata aagtagttgc aattgttttt ttgtgtctta
     1621 ccattctttt tgctattatg gcttatggat tcaatataca aaatccatgg gtgccttttg
     1681 cttttctaac aggtggtttc ttttcagggt tagctggata tataggcatg agaactgcca
     1741 cttatgcttc tgccagagca gctaatgctg cacannnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnatatag gcatgagaac tgccacttat gcttctgcca gagcagctaa
     1921 tgctgcacaa cattcattga atagtggttt aaaaatcgcc tttcgctcag gagcagttat
     1981 gggtttagta gttgtgggac ttggcttgct tgatatttct atttggtttt atgtgctaca
     2041 attttttatt ggtgctttag acaacacaac agatcttgct attgcagccg atccttcaat
     2101 gaaactgatt attattacta ctactacctt aacttttgga atgggtgctt ctacccaatc
     2161 tttatttgct cgtgttggtg gtggaatttt taccaaagct gctgatgtag gtgctgacct
     2221 tgtaggtaaa gtagaagcca atattcctga agatgatcct cgtaatcctg caactattgc
     2281 tgataatgta ggagataatg ttggcgatgt ggctggaatg ggagccgatt tgtacgaatc
     2341 ttattgtggt tcaatccttt ctacggctgc cttaggagcc actgcttttg ctggttttgg
     2401 taccacaacc cagtttaatg gtgttatagc tccaatgatt atcgcctcag taggaattat
     2461 tctttctatt ttaggaatat tcttagtgag atcaaaagaa gatgcttcac aaaaacaatt
     2521 gttaaatgcc ttgaatagag gaatttatac cactgctgtt ttaattgcta ttttttcatt
     2581 ttttattttg aaatatttaa atattccaaa ttctctaggt gtatggggct ctatgttggc
     2641 aggtttatta ggtggcgttg caattggtaa gattactgaa tattttactt cagcagctta
     2701 tcaaccaact cgacgaattt ctgaagcttc aaaaacagga cctgctactg ttatcatttc
     2761 aggaattgga actggtatga tttctaccgc tttaccggta attgtagtag ctatttctat
     2821 tattttagct tattatttcg cttcaggtct tgattttact aatattagta tgggattata
     2881 tggaattgct attgcggctg taggaatgct ttcgacgctt ggcattaccc ttgcttctga
     2941 tgcctatggt cctattgctg ataatgctgg tggaaatgca gaaatgagtg gtttaggacc
     3001 agaagcccgt aaacggacag atgcattgga tgctttagga aatacgactg ctgcaacggg
     3061 aaaaggtttt gctattggtt cggctgcttt aactgctctg gctttaatgg cttcttatct
     3121 tgaagaaaca aaagttgcta ttggacggat tcctgaaaaa gcacaactct tttttgatac
     3181 agtaaatact aatattcatc aagcctctat acctcaactt atggaatttt ttcaggtaaa
     3241 tttaatgaat ccacgggtat tggttggagc ttttataggt gcgatggcag cttttctgtt
     3301 ttgtgggtta acaatgaatg cggtaggaag agctgcacaa agtatggttg aagaagttcg
     3361 ccgtcaattt agagaaattg taggaattat ggaaggcaaa gctgaaccag actatgctcg
     3421 ttgtgttgcc atttcaacta aaggggctca aaaagaaatg gtatttcctt ctttattggc
     3481 tatcttaatt ccaattataa caggtatatt ttttggagtt cctggtgtaa tgggattatt
     3541 attaggtagt gtttccacag gttttgtatt agcaattttt atggcaaata caggaggtgc
     3601 ttgggataat gctaaaaaat atattgaaga aggaaatttt ggtggaaaag gttcagaaaa
     3661 tcataaagca gccattgtcg gtgatactgt tggtgatcct tttaaagata cttcaggacc
     3721 ctcgctcaac attttaatta aactgatgag tatggtttcc attgtaatgg caggattaac
     3781 agtaacctgg agcttaatgt aaaaaaatta aaacgttatc ataaaaaaat ccatatcaca
     3841 aaaaggtatg gattt
//
LOCUS       DAOTKS010000127         3843 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_20009_length_3843_cov_4.221753, whole genome shotgun sequence.
ACCESSION   DAOTKS010000127 DAOTKS010000000
VERSION     DAOTKS010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20009_length_3843_cov_4.221753"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="P5236_03515"
     CDS             <1..425
                     /locus_tag="P5236_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRU63463.1"
                     /translation="AVLEGCVNRIPSIGFSLCSYQAEPDFTNFEPYILQIVQIVLEKG
                     LPDDVCLNVNAPSGKIKGTLITRQCKGFWTQEFAGRTDPAGRAYYWLTGYFENREPEA
                     EDTDEWALSHGYISVVPIKVDMTSYETIELLKQWNLEK"
     gene            439..714
                     /locus_tag="P5236_03520"
     CDS             439..714
                     /locus_tag="P5236_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63464.1"
                     /translation="MKLILAGLLIGLISPILFLWLYLHFFFPLDLPFLEIIKKLFPGI
                     LMGKLLLLSIIPDLFIGFIFYKLEHFKLVSGIVLGSIPYLIISIFMF"
     gene            1372..2514
                     /gene="lpxB"
                     /locus_tag="P5236_03525"
     CDS             1372..2514
                     /gene="lpxB"
                     /locus_tag="P5236_03525"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005657548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HRU63465.1"
                     /translation="MKYFLIAGEASGDLHGAHLMHELKNLDAKAEFCFLGGDLMQAEG
                     GTMVKHYREMAFMGFIAVIKNGKKLMNNLKECKKTLRNFQPDILILIDYPSFNLRIAK
                     FAKKELQLPVCYYISPKVWAWKTFRIKQIKQYVDKMLVIFPFEVDFYAQYHYEVEYVG
                     NPTVDAVFSSLNQNQTFEDFCLFNGIEKKPVIALLAGSRKQEISTCLPKMVETASKFS
                     DFQVIIAGAPGIELEFYQVILKNKNIKVVFEQTYQLLLHAKAAIVNSGTATLETALIG
                     TPQVVVYSVAFGRLAYWAKNIFIKVKYISLVNLIAGREVVKELIAHLFTVENLAKELK
                     KLLYNEAYRTKIQEGYQEIKNILGDKGAAERAARKIIAFCEERKKN"
     gene            complement(2639..>3843)
                     /locus_tag="P5236_03530"
     CDS             complement(2639..>3843)
                     /locus_tag="P5236_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRU63466.1"
                     /translation="RIEDLKKQKMKVLSEQKYELAGPIRDQEKQYQYQLEDALKRWER
                     ESQQHPEIVDEEKVAEVVAMMTGIPVQRIAQAEGLRLRQMQEVLKSKIIGQDEAVEKI
                     AKAIQRNRLGLKDPNKPIGSFIFIGPTGVGKTYLAKMLAEFMFDSPDALIRVDMSEYM
                     EKFSVSRLIGAPPGYVGYEEGGQLTEKVRRKPYSIVLLDEIEKAHPDVFNLLLQVMDE
                     GRLTDSLGRRIDFKNTILIMTSNVGTRQLKDFGKGVGFNTSESPEMDAEYSQGVIQKA
                     LSRTFAPEFLNRIDEVVMFNPLNKESIKLIIDLELKDVYNRVKEMGYQLELTPAAKDF
                     IAERGYDAQFGVRPLKRAIQKYIEGQLADIVLSGEIHPGNTILMDIDTENNTIKPKII
                     ESTPIINN"
BASE COUNT         1259 a          577 c          622 g         1385 t
ORIGIN      
        1 gagctgtact ggaaggttgt gtaaacagaa ttccttcaat aggtttttct ttgtgcagtt
       61 atcaagctga gcctgatttc actaattttg aaccctatat tcttcaaata gttcaaattg
      121 tcttagaaaa aggtttgcct gatgatgttt gtttaaatgt caatgcacca tccgggaaaa
      181 taaaagggac attgataacc cgtcaatgca aaggattttg gacacaggaa tttgctggaa
      241 gaactgatcc agcgggacgt gcctattatt ggttaactgg atattttgag aacagagaac
      301 cggaagcaga agacactgat gaatgggctt taagtcatgg ctatatttct gtagttccta
      361 taaaagttga tatgacgtct tacgaaacta tagagttact taaacaatgg aatttagaaa
      421 aataattaaa tgaagcaagt gaaacttatt ttagctggac ttttaattgg cttgatatct
      481 ccaatccttt ttttatggtt gtatttgcat ttctttttcc cactcgattt gcctttttta
      541 gagatcatta aaaaattatt ccctggaatt ctaatgggga aattattatt gctttccata
      601 attccagatt tgtttatcgg atttattttt tacaagcttg agcattttaa gttagtgtca
      661 ggtattgttt taggcagtat accttattta atcatcagta ttttcatgtt ttaactaatg
      721 aaaatacgaa ttattttata aaaaaacaga ttaacttttt caaccgcaaa gttttttaaa
      781 tattagacat tatgaaattg gttattttaa aataatttta cattttttat ttatttattt
      841 agtttcattt tgaaacaata attttttcct actttttcta tgattcgttt aggataaatc
      901 tgctgaacat taatcaattt atgtttattt ttttacttgc tagtaaccta aatgttaagc
      961 atagaatcct tataaaataa acatcataat tttaaaagaa actggattct gtaaaaaatt
     1021 gaaaatttat acaatttttc gtatgaaaaa tacttttcaa tttttgttat ataaagttta
     1081 caaattgatt acaataatat taataatatt aagtattttt ctacgaaata aaatgtcata
     1141 atactcttat ttacaataat tttatttatt tttaaggaat gatttatgta atcacaaatt
     1201 acaatctata taaatgaaaa gagaatcttg aattaaaaaa ataaattgta atgttcacat
     1261 attcagaact taaataagtt tagttttttc tattagttat taactgttca atggaaaaag
     1321 tatttttaag cttatctatt cctatcgaaa ttgtttttat aagatactga aatgaaatat
     1381 tttcttattg ctggtgaagc atcgggcgat ttacatggag ctcatttaat gcatgaatta
     1441 aaaaatttgg atgccaaagc tgaattttgt tttttaggag gcgaccttat gcaagctgaa
     1501 ggtggaacga tggtaaaaca ttaccgcgaa atggcattta tgggatttat tgctgtgata
     1561 aaaaatggga aaaaattgat gaataattta aaagagtgca agaaaacttt aagaaatttt
     1621 caaccagata ttcttatttt gattgattat ccaagtttta atttaagaat agcgaaattt
     1681 gccaaaaaag aattacagct tcctgtttgt tattacattt cgcctaaagt gtgggcatgg
     1741 aaaactttcc gcataaagca aatcaagcaa tatgttgata aaatgctggt tatttttccc
     1801 ttcgaagttg atttttatgc tcaatatcat tatgaagtcg aatatgttgg aaatccaact
     1861 gttgatgcag ttttttcaag cttgaaccaa aatcaaacct ttgaagattt ttgtcttttt
     1921 aatggaatcg agaaaaaacc tgttatagca ttactggcag gtagtcgcaa acaagaaata
     1981 agtacttgtt taccaaaaat ggtagaaaca gcttctaaat ttagtgattt tcaagttatt
     2041 attgctggag cacctggaat tgaattagaa ttttatcaag ttattttgaa aaacaaaaac
     2101 ataaaagttg tttttgaaca aacgtatcaa ttattactac atgctaaagc tgctattgta
     2161 aattctggaa cagccacgct tgaaaccgca ttaattggaa caccacaagt ggtagtttat
     2221 tctgttgctt ttggccgttt agcttattgg gcaaaaaata tttttattaa agtaaaatat
     2281 atttcattag tgaatttaat tgctggcaga gaagtggtaa aagagttgat tgcccatctt
     2341 tttactgtag agaatttagc aaaggaactg aaaaaacttt tatataatga agcatatcgg
     2401 accaaaattc aagaaggtta tcaggaaata aaaaatatac tgggagacaa aggagcagca
     2461 gaacgagctg cgagaaaaat tattgctttt tgtgaggaac gtaaaaagaa ctaattttaa
     2521 ttaaaatggg gcaccagtat tgattttttt atttatttat gtcaagacaa ttgaggcgaa
     2581 tcagtaaaaa aggtaaagag ctctgataat caaagctcct taccttttta gctaatggtt
     2641 agttatttat gatgggagta gattctataa tttttggttt tatcgtatta ttttcggtat
     2701 caatatccat taaaatagta tttcctggat gaatttcgcc cgataaaaca atatcagcca
     2761 attggccttc gatgtatttt tgaatagcac gttttaatgg acgtacacca aattgagcat
     2821 cataacctct ttcggcaata aaatcttttg cggcaggagt taattcgagt tgatacccca
     2881 tttctttgac gcgattgtaa acgtctttca attccaagtc tataattaat ttgatggatt
     2941 ctttgttcaa tgggttgaac atcactactt catcaattcg gttcaaaaat tcaggtgcaa
     3001 aggtacggct taatgctttt tgaatcacac cttgcgaata ttctgcgtcc atttctggac
     3061 tttcagaagt attaaaacca actcctttac cgaaatcttt taattgacga gttcctacat
     3121 ttgaggtcat aataagaatg gtatttttga aatcaattct tcgacctaaa ctgtcagtca
     3181 atcgtccttc atccattact tgcaaaagca aattgaatac atcaggatgt gctttttcaa
     3241 tttcatccaa taatacaatc gaataaggtt tacgacgcac tttttcagtt aactgaccgc
     3301 cttcttcata accgacatat cctggaggtg cgccaattaa acgagacaca ctgaattttt
     3361 ccatatattc actcatatct acacgaatca acgcatcagg actatcgaac ataaattcag
     3421 ccagcatttt tgccagataa gtttttccga cgcctgttgg tccaatgaag ataaaagaac
     3481 caataggctt attaggatct tttaatccca gtctatttcg ttgaatagct ttagcaattt
     3541 tttctactgc ttcatcttgt ccaatgattt tgctttttaa aacttcttgc atctgacgaa
     3601 gtctcaatcc ttcagcttga gcaatacgtt gtacaggtat acccgtcatc atggcaacca
     3661 cttcggcaac tttttcctca tcaacgattt caggatgttg ttgtgattct ctttcccaac
     3721 gtttaagtgc atcttctaat tgatactggt attgtttttc ctgatcacga ataggacctg
     3781 ccaattcata tttttgttcg gaaagcactt tcattttttg cttcttgagg tcttcgattc
     3841 gtt
//
LOCUS       DAOTKS010000128         3836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_20050_length_3836_cov_3.205766, whole genome shotgun sequence.
ACCESSION   DAOTKS010000128 DAOTKS010000000
VERSION     DAOTKS010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20050_length_3836_cov_3.205766"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="P5236_03535"
     CDS             <1..480
                     /locus_tag="P5236_03535"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRU63467.1"
                     /translation="PTEAEWEYAALGGKNTHNYKYAGSNTLDDVAWYSLNSGGTKHNV
                     KEKLPNELGLYDMSGNVNEWCNDWYDEYYGFPIINEVLTIPDLQIDPKGPDTGTEKVV
                     RGGNFEDDEFWGLSACNVKYRSHINPTGYSYGTYLGNPTIFFTSKNTGFRLVISMNN"
     gene            492..1106
                     /locus_tag="P5236_03540"
     CDS             492..1106
                     /locus_tag="P5236_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRU63468.1"
                     /translation="MKRFILFFVLTLGFSFLQAQTVVKMTLPPQADEPVQVVVLFDEE
                     VPEGIPVVLGLIGYKVTGGIEPYTYEWIQNGEVIGTGDIVVITPAKGDQFSLKATDKN
                     KCYSTTSFSMKVIRKASGQHQPIPGLNIYPTLVKENVIHIDLPENDLPLNANIRIFNI
                     KGVLMFQTFADESFTINVYLPDGVYFVSVATEQFHKVEKIIVQH"
     gene            1119..3740
                     /locus_tag="P5236_03545"
     CDS             1119..3740
                     /locus_tag="P5236_03545"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HRU63469.1"
                     /translation="MKKFILKTHISFYYRLVILLLTGTMNFSIFAETPNPTGAKQFAE
                     DFFKTNAPRFAPGKVYQKPVLRQCYQSASYKATPVFVFQNVEKGFVVVAQNNNRFAVA
                     GYAPEGQFQADSVPPQLSSLLQFYEDSLQIKPAAIQKTSAGEPVMTPLLDEAGISLNQ
                     FSHPEVGGCPTGCVATAFVQIMAYYKYPDKGVGSHCYTHPTYGELCADFGNTTYNWDN
                     PTNEDYKKLSFHVGIAMDMNYCESSYGSTPSRSDYPQAMSDYFRYYLNNGFTDSYFII
                     NEINNRRPAYAELPGDPGHAVVLDGYDTDGLFHINFGWGGRYNGYYVLNNNSTFTVGY
                     KFGTNVSFTCFMTPYPLKTDIQDSLALVAVHNSLNGSTGWDLKQPVSTWKGVLMMNGR
                     VISLKLNNGSYFTYKGSIPSEIGNLTALQMLDLLGQFDGELPASIANLTMLKTLSIYA
                     GAGTLKAQLPENVGNLVNLEILEIPMHAEGTIPSSIGSLTKLQRLVLNSGNLTGNIPN
                     EIGNLKNLTYLNLKGNKLTGSIPTGITNLTQLTEIHLDENQLSGSLPDNIGNLTELVA
                     LTLNDNQLSGNLPESMGNCSKLTTLHLYNNQFTGEIPASLGNLSLIKNLNFSNNKFIS
                     LPDEIDNWNNIVELNLNNNQLSSLPRSINNLTNLKTLYANHNQLAELPENFGAFPSLN
                     NIDLSYNQLKVFPEELCLLTKLEHISFRKNKIKEFPAGITMLPTTLNYLALDSNEIKD
                     RIPKALLENENLSSLLLSYNRFTFEDLPVSDQLRNGIGNQKPVNLSKKIFKTAIGDTL
                     RIDIREIAPFTLTTNQYNWISAENNKAISDSPNPILTIIINDKNIKNKYYCKVTNPSS
                     PTYTYVSSGTPFRLPCF"
BASE COUNT         1289 a          690 c          686 g         1171 t
ORIGIN      
        1 ccgacggaag cagaatggga atatgcagct ttaggtggta aaaatacaca taattataaa
       61 tatgcgggaa gcaacacatt ggatgatgtt gcgtggtatt ctctaaactc aggtggtacc
      121 aaacataacg tgaaagaaaa actgccaaac gaactgggac tttacgatat gagtggaaat
      181 gttaatgaat ggtgcaatga ttggtatgat gaatattatg gatttcccat tataaatgaa
      241 gtgttaacaa ttcctgattt acaaattgat cccaaaggac ctgacactgg aactgaaaaa
      301 gtagtcagag gcggtaattt tgaggatgat gaattttggg gactttctgc ctgcaacgta
      361 aaatatcgtt cacacataaa tcctacaggc tatagttatg gtacttatct aggtaatcca
      421 acaatatttt tcacaagtaa aaatacagga ttccgattgg ttatttcaat gaacaattaa
      481 aaactaaaat catgaaaaga tttatattat tttttgtatt aacgcttggc ttctcttttc
      541 tgcaagctca aaccgttgta aaaatgactt taccgccaca ggcggatgaa ccagtgcagg
      601 tagttgtatt gttcgacgaa gaagtaccag aaggcattcc tgtagtgctt ggtttaatcg
      661 gatataaagt gacaggtggt atagaacctt acacttacga atggattcaa aatggtgaag
      721 ttatagggac aggcgatatt gtggtaatta ctcctgcaaa aggagatcaa ttttcattga
      781 aagctactga taaaaacaaa tgttattcca ctacttcctt cagcatgaaa gtcatcagaa
      841 aagcaagtgg acaacatcaa cccattcctg gcctgaatat ttatcctacc ttggtaaaag
      901 aaaatgtaat tcatatcgat ctgccagaaa acgatttacc tctcaatgcc aacattcgta
      961 tattcaacat aaaaggagta ttgatgttcc aaacttttgc agatgaaagt tttacaataa
     1021 atgtatattt acctgatgga gtttattttg tttcagtagc cactgaacaa ttccacaaag
     1081 tggagaaaat tatcgttcaa cattaaaaaa cattagcaat gaaaaaattt atattaaaaa
     1141 ctcatatttc attttattat aggttggtaa ttctgttatt aacaggaaca atgaatttct
     1201 ccatttttgc tgaaactccc aatccgacag gtgcaaaaca atttgctgaa gattttttca
     1261 aaaccaatgc tccccgcttt gctccaggaa aagtttatca aaagcctgtg ctgagacagt
     1321 gctatcaatc agcaagttat aaagcaacgc ctgtgtttgt ctttcaaaat gtagaaaaag
     1381 gatttgttgt tgttgcacaa aacaataatc gttttgctgt agcaggttat gctccagaag
     1441 gtcaatttca agctgatagt gttccacctc aattatcctc tttgcttcaa ttctatgaag
     1501 attcgttaca aattaagcca gcagccattc aaaaaacctc agcaggagaa cctgttatga
     1561 cgcctttact ggacgaagca ggcatatctc ttaatcaatt tagccatccg gaagtgggag
     1621 gatgtccaac gggctgtgta gcaaccgctt ttgttcaaat tatggcttat tacaaatatc
     1681 ctgataaagg tgttggctca cattgttata cgcatcccac ttatggagag ctttgtgctg
     1741 attttggaaa tacgacctac aattgggata atccaaccaa tgaagactat aaaaaacttt
     1801 cgtttcatgt aggtattgca atggacatga attattgtga aagcagttac ggcagtactc
     1861 cctctcgatc ggattacccc caagccatga gtgattattt tagatattat ctcaataatg
     1921 gctttacaga ctcttatttt attataaatg aaattaacaa ccgacgtcct gcttatgctg
     1981 aacttccggg cgatcccgga cacgcagtag ttcttgatgg ctacgatacc gatggcttgt
     2041 tccatattaa ttttggttgg ggtggacggt acaacggtta ttatgtacta aataataatt
     2101 ccacgttcac tgttggatat aaatttggaa cgaatgtttc ttttacctgt ttcatgacac
     2161 cttatccgtt aaaaaccgat atacaggatt cgttggcact ggtagctgta cacaattcat
     2221 tgaatggctc caccggctgg gacttaaaac aacctgtaag tacatggaag ggtgttttga
     2281 tgatgaatgg aagagtgata agtttaaaat taaacaatgg aagttatttt acatataagg
     2341 gaagtattcc ttcagaaata gggaatttaa ctgctcttca aatgttagat ttattgggac
     2401 aatttgatgg tgaattacca gcttctatag ccaatttaac tatgttgaaa acgctcagca
     2461 tttatgcagg agcaggaaca cttaaagcac agttgcctga aaatgttggt aatttggtaa
     2521 atctggaaat attggaaatt cctatgcatg cagaaggcac tattccttct tccattggca
     2581 gcctcacaaa attacagcga ttggttttaa attctggaaa tttaaccgga aatattccca
     2641 atgaaattgg caatctgaaa aacctgacct atttgaattt aaaaggaaac aaattaacag
     2701 gttcaattcc aacaggaatc accaatttaa cccaacttac tgaaattcat ttagatgaga
     2761 atcagttaag tggttcttta ccagataata ttggcaattt aacagaactc gttgctttga
     2821 cgttgaatga caatcaacta tcgggcaatt tacctgaaag catgggcaat tgcagcaaac
     2881 tcacaacatt gcatctctat aacaatcaat ttaccggtga aattcctgct tcgctgggta
     2941 atttaagttt gataaaaaat ttgaatttca gcaataataa atttatttct ttgccagacg
     3001 agatagacaa ctggaacaat atagtagaat taaatctgaa taacaatcaa ttatcttctt
     3061 taccaagatc gatcaataac ttgacaaatt taaaaacatt atacgccaat cataatcaac
     3121 ttgctgaatt acctgaaaat tttggggcgt tcccttcact taataatatc gatttatcat
     3181 acaatcaatt aaaagtattt cctgaagaac tatgcctgct cacgaaattg gaacacattt
     3241 cgttcaggaa aaataaaata aaagaattcc ctgccggcat taccatgttg cctactacgc
     3301 tcaattacct ggctttagat agtaacgaaa taaaagatcg tataccaaaa gctttgctgg
     3361 aaaatgaaaa tctatcttct ctgttgctct cttacaatcg ttttactttt gaagatttgc
     3421 cggtatctga tcaactaaga aatggtattg gcaatcaaaa gccagtaaac ctatcgaaaa
     3481 aaatatttaa aactgcaatc ggcgacaccc tccgtattga tattcgagaa attgcacctt
     3541 tcacattgac taccaaccaa tataattgga tctccgcaga aaataataaa gctataagtg
     3601 attcacctaa cccaattttg accataatta ttaatgataa gaacattaaa aacaaatatt
     3661 attgtaaggt aaccaatcct tcatcgccaa cttacactta tgtatcttct ggaactccgt
     3721 tccgtttgcc atgtttctga ttgaaggagc tctgagtatg gctaagaaga tttgcattcg
     3781 atacaagaac ttcgatcttt tgagaataat cccatttcct gctgggagcg aatatg
//
LOCUS       DAOTKS010000129         3831 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_20079_length_3831_cov_5.262182, whole genome shotgun sequence.
ACCESSION   DAOTKS010000129 DAOTKS010000000
VERSION     DAOTKS010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20079_length_3831_cov_5.262182"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="P5236_03550"
     CDS             complement(<1..391)
                     /locus_tag="P5236_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63470.1"
                     /translation="MKTKARLNIRRSDDLKTSKSFSIFFRISFGASDFDDKKKYVVLN
                     YFTGLQTIAKYWKVKDGRSKTDSYLNGVLERANVRIHALWSEMNYNGKPNIDVFKKTL
                     ADDKELNEILNRKSVAVKGYVSPFDFIE"
     gene            complement(756..3218)
                     /locus_tag="P5236_03555"
     CDS             complement(756..3218)
                     /locus_tag="P5236_03555"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRU63471.1"
                     /translation="MKKLIFLFFLNFFIGFTLSAQLLTTVKGQVVGPTENEPLPYVTI
                     SIAKDNNPEISIKKFATDIKGNFVISIPPGKYIFTFQFVGMNVLKKNVEVAGQTEIDL
                     GKIILEESSTQLNEINVVAQKPLVKVETDKITYNAKDDPESSTSNVLELLRKVPMVTV
                     DGEDKIQLKGSNNFKIYLNGKPSNMMTNNPSQVLKSMPASSVKNIEVITEPGAKYDAE
                     GVGGIINIVTDKRADDGYSGSIGANADIRGGYGGNGYLALKYGKVGFTGNASYYHFNQ
                     PDVESNYERHEFDSSLENLLKQDGTSKVNGGGLYLSGALSYEPDTMNLLNFSASRYGG
                     NYLVNTSQDALSQGIQNYSYHSKSSSTTEYGGINLSGDYQHNFKKKGEMLTVSYRYEE
                     NPNNSNFDNLFTDVSGNYFYPDGYRQQSINKATGKEHTGQVDYEIPFPQKHIFETGMK
                     YIFRKNYSTTDNSYYDVINEIWKTDTTQKNDLNHHQNILSGYVNYSFKTGKFGLNVGF
                     RGEYTFQRIHFMNSQDTIINTNFFDPVPSITVSYQLGMTKTLRAGYNMRISRPGIWYL
                     NPYVNNLNPTDIYYGNPELDAEHTHNFNINFGSFSQKTNLNTTLSYSLTNNAITSYSF
                     IKDGITHNTQANIGKNQNISLNIFGSWTPNQKLRLYANANINYTDIRSTNNTQLQNDG
                     FSGQIFSGLTLNLPKDFRLGANGGIFNSYIQLQTTQSSFYFYSFNLMKSLFNKKLDIN
                     LSTVNPFPESIAIKSQTKGVGFLQKTTYSEPIRNVRLSATFRFGDLKTTIKKVKKTIS
                     NDDIKAGGSDTQKGTGVIPAGN"
BASE COUNT         1247 a          603 c          553 g         1428 t
ORIGIN      
        1 cttctataaa atcaaatgga gaaacatatc ctttcactgc tacggacttc ctatttaaaa
       61 tttcgttaag ctccttgtcg tctgccagtg ttttcttaaa aacgtcaata ttaggcttac
      121 cgttgtaatt catttcactc caaagtgcat gaatgcgaac gttggcacgt tccagcactc
      181 cgtttagata actgtcggtt ttacttctgc catctttaac cttccaatac ttggcaatgg
      241 tttgcaggcc tgtaaaataa ttcagtacaa cgtatttttt tttatcgtca aaatctgatg
      301 ctccgaaact aattctgaaa aatatggaaa aagattttga agtttttaag tcatcacttc
      361 tccgtatgtt taacctcgct tttgttttca tctttctatt tttttacaaa gatagaattt
      421 acaaacgaat ttacaaacga atttatgata attcttttta tttttcttta ctattcttta
      481 gaaatgcgat ttatatattt atgtttatca gtctgttata aaacgcatta acacaaaata
      541 actatcatac agtatcataa gttcaagtcc tagtgagaca gcaatattgc aaaaaattta
      601 aagtatttca aacaaatttt caaaagtcat cgacttgctc tctaaaattc acgtaatttt
      661 caataattag tttcttcctt ttctgtttca attctggcag tattattcat aaatttttcg
      721 gtcaattgtt agttttattg caactattgc tattattaat ttccagcggg aataacccct
      781 gtaccttttt gagtgtcact gccacctgct tttatatcat cattgctaat ggtttttttg
      841 actttcttaa tggtggtttt taaatcacca aatcggaaag tggcacttaa cctcacgttt
      901 cgaattggtt ctgaataagt agttttttgc aggaaaccta cccctttagt ttgagatttg
      961 attgctatcg attcaggaaa aggattaaca gtagaaagat taatatctaa tttcttatta
     1021 aataagcttt tcatcaagtt aaatgaataa aaataaaagg aggattgggt ggtttgcaac
     1081 tgaatataac tattaaaaat gcctccattt gctcctaaac gaaaatcttt cggtaaattt
     1141 agtgttaatc cgctaaaaat ttgccccgag aagccatcgt tttgtaattg agtattattt
     1201 gtactcctaa tatcagtata atttatattg gcattggcat acaaacgtaa tttttgatta
     1261 ggagtccaac ttccaaaaat gttcagactg atattttgat ttttcccgat atttgcttgt
     1321 gtattatgag ttattccatc ttttataaaa ctataagaag ttatagcgtt attggttaaa
     1381 gaatagctca aagttgtgtt taaattggtt ttttgagaaa aagatccaaa attgatatta
     1441 aaattgtggg tatgttcggc gtccaactca ggatttccat aataaatatc ggttggatta
     1501 agattgttaa cgtaaggatt tagataccaa ataccagggc gtgaaatacg catgttataa
     1561 ccagcacgta aagtttttgt cattcccaac tgataagaaa ctgtaattga tggcacggga
     1621 tcaaaaaagt ttgtattgat aatagtgtcc tgagaattca taaaatgaat tctttgaaag
     1681 gtatattctc cgcgaaaacc gacatttaaa ccaaatttcc cagttttgaa actgtaattt
     1741 acatagccag atagaatatt ctgatggtga ttcaaatcat ttttttgagt cgtatctgtt
     1801 ttccaaatct cgttgataac gtcataataa gaattatcgg tggtactgta attttttctg
     1861 aaaatatatt tcattccggt ttcaaaaatg tgtttttggg gaaatggaat ttcataatca
     1921 acttgtcctg tatgttcctt gcctgttgct ttgttaatgc tttgctggcg gtaaccatca
     1981 ggataaaaat aatttcctga aacatctgta aatagattgt caaaattact gttgttggga
     2041 ttttcttcgt aacggtaaga aacagttaac atttctcctt tctttttgaa attatgttga
     2101 taatcgcctg acaaattaat tccaccatat tcagttgttg aagatgattt ggaatgataa
     2161 ctgtaatttt gaatgccctg agataaagca tcttgcgatg tatttacaag ataatttcct
     2221 ccatatcgag atgctgaaaa gtttaaaaga ttcatcgtat ctggttcata acttaaagct
     2281 ccactcaaat ataagcctcc accatttact ttacttgttc catcttgttt cagcaaattt
     2341 tcaagtgaag aatcaaattc gtgcctctca tagtttgatt ctacatcagg ttgattgaaa
     2401 tgataataac tggcatttcc cgtgaatcca acttttccgt attttaatgc cagatagcca
     2461 ttccctccat atcctccacg aatatcagca ttcgctccaa ttgatccaga atatccatca
     2521 tcagcccttt tatcggtaac aatattaata attccaccta ctccttccgc atcatatttt
     2581 gcacccggtt cggtaataac ttcgatattc tttacgctac tggcaggcat gctttttaat
     2641 acttgtgaag gattattagt catcatatta gaaggcttac cattcagata gattttgaaa
     2701 ttgttggaac ctttcaactg gattttatct tctccatcaa cagttaccat tggtactttt
     2761 ctcaaaagct ccagaacatt ggaagtagag gattcaggat catctttggc attgtaagta
     2821 atcttgtctg tttctacttt tacaagcggt ttctgggcaa caacatttat ctcattaagt
     2881 tgtgttgaac tttcttcaag aataatcttt cctaaatcaa tttcggtttg tccagcaact
     2941 tccacatttt tttttaaaac attcattcca acaaactgaa aggtaaaaat atatttacct
     3001 ggaggaatag aaatcacgaa atttcccttt atatcagtag caaatttttt aatggatatt
     3061 tctggattat tatcctttgc tatagaaata gttacataag gaagcggctc attttcagtt
     3121 ggacctacta cttgaccttt tactgtagtt agcaattgag ctgataaagt aaaaccgata
     3181 aagaaattta aaaaaaataa gaaaattaat tttttcatta aagtttttta caaaggattt
     3241 atttaaattt aaataaaaga aaaatattcc aaactacgga ttcattaata atataacatg
     3301 atcctcttaa aaaaatcaat tttaataatt tagtgatttg caagtcattg agtattaata
     3361 ttgaatttcc ttagaaaatt tataaaacag gtttttaaag gtagattcat aaaaacaatt
     3421 tttaaatttg taaaattcgg aataaagatt cattaccaaa taaaaaaaat aatattttta
     3481 gtaatattta attgattttt tattatacag aatattagca tcaaagtacc agtaaaaagt
     3541 tcttgcaaaa gaaaaaataa ttttactttt cgctcttctt ataaatggta ttaattactt
     3601 gaatttatct tgttattttt ctaaaatttt ttaatttttg tagaatagtt tttgtcacta
     3661 aaaatatatt aacaatcaaa tgtttttcgt taaaaacaag taaaagatga aggattcaat
     3721 ttagtgagac attatttgtt tagttaaaaa attatatctt tgcttttaaa tttttatcaa
     3781 agaacaaatg aaaacagctt cgaggacaat tctttttatt gctttatttt t
//
LOCUS       DAOTKS010000130         3735 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_20696_length_3735_cov_4.881250, whole genome shotgun sequence.
ACCESSION   DAOTKS010000130 DAOTKS010000000
VERSION     DAOTKS010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20696_length_3735_cov_4.881250"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="P5236_03560"
     CDS             <1..464
                     /locus_tag="P5236_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018464880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRU63472.1"
                     /translation="FIFQFHQLLPEFTALENVMIPALIAKKDAKETVKRAKEILSELQ
                     LADRMEHKPNELSGGERQRVAVARALINQPSVILADEPSGSLDSKNKIELHQLLFDLR
                     AKFGLTIVIVTHDKELAKLTDRVIELKDGMIQNVNNNGKVVLPGIEPESKV"
     gene            complement(427..498)
                     /locus_tag="P5236_03565"
     tRNA            complement(427..498)
                     /locus_tag="P5236_03565"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(463..465),aa:Arg,seq:cct)
     gene            589..1221
                     /locus_tag="P5236_03570"
     CDS             589..1221
                     /locus_tag="P5236_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HRU63473.1"
                     /translation="MSKNKFYVVWKGKQPGIYSSWEECKLQIDGIKDAIYKSFLTEEE
                     AIEAFKSPSSNYIVKNLSFSEKKSDEKNSLLQPILESISVDAACSGNPGILEYRGVYT
                     RTGKEIFHQGPFQNGTNNIGEFLAIVHALALQMKKNTSFPIYSDSATAIKWVKTKKAK
                     TKLVESPENAYLFELVERAEKWLEQFEYSIPVLKWQTENWGEIPADFGRK"
     gene            complement(1229..2620)
                     /locus_tag="P5236_03575"
     CDS             complement(1229..2620)
                     /locus_tag="P5236_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63474.1"
                     /translation="MIRKLSAGDRFVISNFLVAFVFFWIIRAIYHFTTITNEQNIPFL
                     TIIAILFFIINLTLSYFIFKKERAKIKFSWEFILNMALLFLIISTVLSVYTSIVATVA
                     FAGLVIIYASKKKIHFQPHPLFYFLVAYYLLQYIGLIWSIDPQNGFRFVGKGLSFIIV
                     PLAFSFYSISEDEKRKLLRIFFRLLEIYLLMILLAYCYQVYFHKVNFGIGFHLKKNYF
                     PTPIPPGETHHLLLAWAGYYHPTFVSFILTLMFGIGFYLWRNDNSPQTVRISGFEVGF
                     YGFMTAGVILLLQSRIGMLMFPFGIFLTLLYSLRKNKKLVLSVIVFSALLVLLAGFYI
                     FKNYRGYFYDHPREIQTDIVLSFLKDHYLLGTGTGGMKVLIPWYPTAHNQFLGDLFHL
                     GIPGLLLLLALMGSSFYFAIKDKNFPLLYFLLIYFVMMQIEMPLSLQKGITYYTLFVC
                     LFLNGKKKATTKE"
     gene            complement(2639..>3735)
                     /locus_tag="P5236_03580"
     CDS             complement(2639..>3735)
                     /locus_tag="P5236_03580"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HRU63475.1"
                     /translation="KKYRKYKKTLLARKNIIDACALYRSADALKIGGYNEEIIGREDW
                     EFWISMLKNGGEVYHLPEVGVYYRIRQHSKRVTDRRLKKHIIDVLNRRHKAFFYRELG
                     GKLHYQRSLSRLLNFFIHLFRPEAFVISPDFKKEEEFFYSLPDIFPHQGETIHSGRNI
                     IKIFSIGTQKWVVKWFKSPNFFNRFIYGNFRKSKARRSYEYALKLQSLGIKTPVPIGY
                     YEQKNLFFFSQSFYATLASECEHRFDEIIANKDFKDRELILKEFAKFSAKLHENNVLH
                     GDYSEGNILFSIKGKEVLFEIVDLNRIKFKKVSPEEGCKNFERLNLEPEALTIIAKAY
                     ARARNLDEKNCIDWILKYRWRKHKNISKTI"
BASE COUNT         1395 a          610 c          585 g         1145 t
ORIGIN      
        1 gattcatttt tcaatttcat caattgttac ctgaatttac tgctcttgaa aatgtgatga
       61 ttccagcttt gattgcaaaa aaggatgcta aagaaactgt aaaaagagca aaagaaattc
      121 taagtgagtt acaattggca gaccgaatgg aacataaacc aaatgaatta tcaggcggag
      181 aaagacaacg agtagcggta gctcgggctt tgataaacca gccttcagta attttggctg
      241 atgaaccatc aggcagtttg gattcaaaaa acaaaattga attgcatcaa ttgttgtttg
      301 atttacgagc aaagtttgga ctgacaattg ttattgtaac acacgataaa gaattagcaa
      361 aattaactga cagagtaatt gaactcaaag atgggatgat ccaaaatgta aataacaatg
      421 ggaaagtagt cctgccggga atcgaacccg aatctaaagt ttaggaaact tttgttctat
      481 ccattgaact acaggacctt ttgggaaagt agtgcaaaat tacgtttttt taatgagaga
      541 gaaaagcatt ctatcctatt ttgaaaccat tgtataaggt taaattttat gagcaaaaat
      601 aaattttatg ttgtctggaa aggaaaacaa cctggtatat attcttcgtg ggaagaatgt
      661 aaattgcaaa tcgatggtat aaaagatgct atttataaat cttttcttac agaagaagaa
      721 gcaattgaag cctttaaatc tccttcctca aattatatag tgaaaaatct ttctttttca
      781 gaaaaaaaat cagatgaaaa aaatagctta cttcaaccaa tcttggaaag tatttccgta
      841 gatgcagctt gtagtggaaa tcctggtata ctcgaatatc gaggagttta taccagaaca
      901 ggcaaggaaa tctttcatca aggaccgttt caaaatggaa caaataatat cggtgaattt
      961 ttagcaattg tgcacgcact ggctttacaa atgaaaaaaa atacttcttt cccaatttat
     1021 tctgatagtg caactgccat aaaatgggtg aaaacaaaaa aagcaaaaac aaaattggtt
     1081 gaatcacctg aaaatgcata tctctttgag ctagttgaaa gagctgagaa atggttggaa
     1141 caatttgaat attctattcc tgttttaaaa tggcaaaccg aaaactgggg tgaaattccg
     1201 gctgattttg gaagaaaata gaaaattttt attctttagt tgttgccttt ttctttccat
     1261 taagaaataa acagacaaat aaagtgtaat aagttattcc tttttgtaaa gaaagcggca
     1321 tttctatttg catcataaca aaatagatca acagaaaata cagcaacgga aaatttttat
     1381 ccttaatggc aaaataaaac gaacttccca ttaaagccag taacaagagc aagccgggga
     1441 tacctaaatg gaacagatct cctaaaaatt gattgtgagc ggttggatac cagggaatta
     1501 aaactttcat tccccctgta ccagtgccta ataaataatg atcttttaaa aacgaaagta
     1561 caatgtcagt ttgaatttcg cgaggatggt cgtaaaaata cccacgataa tttttaaaaa
     1621 tataaaatcc ggctaacaga actaaaagag ctgagaaaac aattactgaa agaacaagct
     1681 ttttattttt tctcaagctg taaagcaagg ttaaaaaaat tccaaacgga aacataagca
     1741 tgcctatacg actctgcaac aataaaataa ctccggctgt catgaaccca taaaaaccta
     1801 cttcgaaacc ggatattcta actgtttgcg gtgaattgtc gtttcgccac aaataaaacc
     1861 cgattccaaa catcagcgtc aaaatgaatg aaacgaaagt cggatgataa taaccagccc
     1921 acgccaacaa taaatgatga gtttctccag gaggaatcgg agttgggaaa taattttttt
     1981 tcagatgaaa cccaattcca aaatttactt tatgaaaata tacttgataa caatatgcaa
     2041 gtagtatcat taataaataa atttccaaca agcggaaaaa aattcttaac aatttccttt
     2101 tttcgtcctc tgaaatggaa taaaaactaa atgccaatgg tacaattata aaagataatc
     2161 ccttccctac aaaccggaaa ccattctgcg gatcaataga ccaaattaac ccaatatatt
     2221 gaagtaaata ataagctact aagaaataaa acaatggatg aggctgaaaa tgaattttct
     2281 tttttgaagc ataaattatt accaatcctg caaaagcaac tgttgctaca atgctggtat
     2341 agacacttaa aactgttgag ataattaaaa acaacaaagc catgtttaat ataaactccc
     2401 atgaaaattt tattttagct ctttcttttt taaatataaa ataagataag gttagattaa
     2461 taataaaaaa caaaatggct attattgtga gaaaaggaat gttttgctca ttggtaatgg
     2521 tggtaaaatg atagattgcc cgaataatcc agaaaaaaac aaaagcaacc aaaaaattac
     2581 taataacaaa acgatctcct gcagaaagtt tacgaatcat aattcaactt ttaaatgatc
     2641 agatagtttt tgaaatgttt ttatgttttc tccagcgata ttttaaaatc cagtctatac
     2701 aatttttttc gtccaaattt cgtgctctgg catatgcttt tgctataata gttaaagctt
     2761 caggttctaa atttaatctt tcaaaatttt tgcaaccttc ttcgggtgat acttttttaa
     2821 attttatccg attcaggtca actatttcaa ataaaacttc cttccctttg atagaaaata
     2881 aaatatttcc ttcagaataa tcaccatgca aaacattatt ctcgtgcaat ttagcagaaa
     2941 actttgcaaa ttcctttaaa ataagctccc tatctttaaa atctttattt gcaatgattt
     3001 catcaaatcg atgctcacat tcagatgcca aagtagcata aaaactttga gaaaaaaaga
     3061 acaaattttt ttgttcataa taaccaatag gaacgggtgt ttttatccct aacgattgaa
     3121 gtttcaaagc atattcataa gatcttcgtg ctttcgattt tctaaaattt ccataaataa
     3181 agcgattaaa gaaatttggt gacttgaacc atttcacgac ccatttctgg gttccaattg
     3241 aaaaaatttt aatgatattc cttcctgaat gaattgtttc gccttgatga ggaaaaatgt
     3301 ctggcaaaga ataaaaaaat tcttcttctt ttttaaaatc aggtgaaata acaaaagctt
     3361 caggtctaaa aagatgaatg aaaaaattta ataagcgtga taacgagcgt tgataatgca
     3421 attttcctcc caattcgcga taaaaaaatg ctttgtgacg acggtttaaa acgtcaataa
     3481 tatgtttttt taaacgacga tcggtcactc gttttgaatg ctgacgtata cgataataaa
     3541 caccaacttc cggtaaatga taaacttccc caccattttt taacatcgaa atccaaaatt
     3601 cccaatcttc tcgtccaatg atttcttcgt tataaccgcc aattttcaaa gcgtctgcgc
     3661 ttctatacag ggcacaggca tcaataatgt tttttcgagc gagcaatgtt tttttatatt
     3721 ttctatattt tttta
//
LOCUS       DAOTKS010000131         3724 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_20772_length_3724_cov_3.864541, whole genome shotgun sequence.
ACCESSION   DAOTKS010000131 DAOTKS010000000
VERSION     DAOTKS010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20772_length_3724_cov_3.864541"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="P5236_03585"
     CDS             complement(<1..923)
                     /locus_tag="P5236_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRU63476.1"
                     /translation="MEQSNKLKIINDPVFGFINIPDDFLYEIIQHPYFQRLNRIKQLG
                     VSYYVYPGAQHTRMQHSLGCMYLVGEAIKELRQKGHFITTGEEQAVRACMLLHDIGHG
                     PFSHALEKSLVTDIDHEKISLLMMQRLNEEFEGKLTTAIEIFTNQYPKKFLHQLVSSQ
                     LDMDRMDYLSRDSFFCGVSEGIIGSARIINMLNIFNDDLVVESKGIYSIEKFLVARRL
                     MFWQVYLHKTCVAVERMLINILTRAKELVLSGKKLFSSPALHYFLYNYINEEKFSESV
                     EVLDNFANLDDSDLICAIKVWTNNEDKVLSY"
     gene            complement(967..2412)
                     /locus_tag="P5236_03590"
     CDS             complement(967..2412)
                     /locus_tag="P5236_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HRU63477.1"
                     /translation="MINIEPSTVWYYFNEITKIPRPSKKEEKIRNYLLQIAKLYNLSA
                     KVDRAGNVLITKPATSGYENQQKIILQSHMDMVCEKNSEVDFDFETDAIQTYIEGDWI
                     KAKGTTLGADNGIGMAMMLAVLTSNDISHPEIECLFTVDEESGLTGASALEPDFLEGK
                     ILINLDSEDDGVIFIGCAGGIDTTANLSYIPRKTPLTNLSFSITVKGLKGGHSGEDID
                     KKHGNAIKILTRFLWNLSKEVVVHLHSLEGGGLSNAIPREATAIISIPFHQKENLSVL
                     FNQYIAEITSELQQSEPNLQFILSSEKMPYTVLDKRSSKTLLNALYACPHGVKARSEE
                     IPDLVETSTNLASVKMIDNNRILITTSQRSSVESAKYDIAHQVESVFVLAGAKVHHSK
                     GYPGWKPNFNSEILKVAQQCYQEITGEEPKVRAVHAGLECGLFLEKYPQLDMISIGPQ
                     MYGVHSPEERLSISSTQKCWKWLVKILAHRL"
     gene            complement(2569..3435)
                     /locus_tag="P5236_03595"
     CDS             complement(2569..3435)
                     /locus_tag="P5236_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63478.1"
                     /translation="MSLNIWCHYLKLLFFIFLILVIVSCEKSDNEVTNSAYITDVFEY
                     QYAPGQHANTVKPADAANFISLPSNIKSCLYLGGWGGYVIAGFDHNVINHEGYDFEVF
                     ALAGVSPEPAIVYVMEDKNGDGKPNDIWYELKGNQFENSKRNYWVCYYKAFSDSANIT
                     WKDSEGNTGELLAGFGTKYTASWWWKYYENDSITFYGTRLPDAYENNSTQWVVPVDRF
                     TWGYAENNFGIDYDQIVHSNQFDISNAVDSLGNSIDLLHIRFIKIQTGVFQQAGWLNE
                     ISSEICGARELK"
     gene            complement(3464..>3724)
                     /locus_tag="P5236_03600"
     CDS             complement(3464..>3724)
                     /locus_tag="P5236_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HRU63479.1"
                     /translation="HIEQLIDNPAGMSNFEMLQLQLNKCREVLEMNKDNKGQKIVFIH
                     GKGEGVLKKEIQKLLETQYKKFYFQDASFQQYGFGATMITIR"
BASE COUNT         1211 a          687 c          557 g         1269 t
ORIGIN      
        1 aaatatgata aaactttatc ttcgttattt gtccaaactt taatggcaca aatcaggtct
       61 gagtcgtcaa gatttgcaaa attgtcgagt acttcaaccg attcactaaa tttttcttca
      121 tttatataat tgtataaaaa ataatgcaaa gctggtgaag aaaaaagttt tttaccgctc
      181 aataccaact ctttggctct tgttagtata ttgattagca ttctttcaac agctacacat
      241 gttttatgca aataaacttg ccaaaacatc aaacgtcgtg ccacaaggaa tttttcaata
      301 gaataaatgc cttttgactc aactaccaga tcgtcattaa atatatttag catattaata
      361 atgcgagcag aaccaataat tccttcactt acaccacaaa aaaaactgtc acggctcaaa
      421 taatccattc tgtccatgtc cagttggctt gaaaccaatt gatgtaaaaa tttttttgga
      481 tattgattgg taaaaatttc aatcgcggtg gtcaatttcc cttcaaactc ttcattgaga
      541 cgctgcatca tcaaaagaga aattttttcg tggtcaatgt ccgtgaccaa acttttttcc
      601 agtgcatggg aaaaaggtcc gtgtcctata tcgtgtaaca gcatacaagc ccgaactgct
      661 tgttcttcgc ctgtagtaat gaagtgacct ttttgtctca actctttaat ggcttcacca
      721 accagataca tacaaccaag tgaatgttgc atgcgggtat gttgggcgcc aggataaaca
      781 taataagaaa cgcccaattg tttaatacga tttaatcgct gaaaataagg atgttgaatg
      841 atttcatata aaaaatcatc aggaatattg ataaaaccaa aaacaggatc gttaattatt
      901 tttaatttat tcgattgttc catttttttt tatctatgct gattaaatga aatattgaga
      961 gaacgtttat aacctatgtg ccagtatttt caccagccat ttccagcatt tttgagttga
     1021 agatatactc aatctttctt ccggagaatg aaccccatac atttgtggtc caatagaaat
     1081 catatccaac tgaggatatt tttcaagaaa aagtccacat tccagacctg catgtactgc
     1141 tcgaacttta ggctcttctc ctgtaatttc ctgataacat tgttgagcta ctttaaggat
     1201 ttcagaatta aaatttggct tccatcctgg ataaccttta ctatgatgta cttttgctcc
     1261 agccaaaaca aaaaccgatt ctacctgatg agctatatca tatttggctg attcaacaga
     1321 acttcgttga ctggtagtaa tcaaaattcg gttattatct atcattttta ctgaagctag
     1381 attagtagaa gtctcaacta aatcgggaat ttcttcactt ctggctttta ctccgtgagg
     1441 gcaagcataa agagcattaa gaagtgtttt tgagcttctt ttatccaaaa cggtatatgg
     1501 cattttttcc gaagaaagta taaattgcaa attaggctcg ctttgttgta attcagatgt
     1561 tatttctgca atatattgat tgaataaaac actcaaattt tctttttggt gaaaaggaat
     1621 agaaattatt gcagtagctt cacgtggaat ggcattggat aaaccaccac cttccagact
     1681 atgaagatgt actactactt ctttgcttaa attccacagg aatcgagtca aaattttaat
     1741 agcattgcca tgtttcttgt caatatcttc ccctgaatgt cctcctttta gacctttaac
     1801 tgttatggaa aaagataaat tagttagcgg agtttttcga ggtatatatg aaagattagc
     1861 tgtggtgtca attccaccgg cgcaacctat aaaaataaca ccatcatcct ctgaatccaa
     1921 attaattaaa atttttcctt ccaaaaaatc aggttctaaa gctgaagcac cagttaaacc
     1981 actttcttca tcaacagtaa acaagcattc tatttccgga tgggaaatat cattggaagt
     2041 caagacagcc agcatcatag ccataccaat tccattatcc gcacctaaag ttgttccctt
     2101 tgctttaatc cagtcacctt ctatgtaagt ttgaatagca tcagtttcaa aatcgaaatc
     2161 cacttcacta tttttttcac ataccatgtc catatgtgat tgtaaaataa ttttttgctg
     2221 attttcgtaa cctgaagtgg ctggtttagt aatcaaaaca tttcctgccc tatcaacttt
     2281 tgctgaaaga ttatatagct ttgcaatttg aagaagataa tttctgattt tttcttcctt
     2341 ttttgaagga cgaggaattt tagttatttc gttaaaataa taccagactg ttgaaggttc
     2401 gatattgatc ataatgtagt tttgagtttt gaatttctag ttacaaattt cataggaaat
     2461 gaaaatttaa caaagataat taaaatttca tagatagaag ctaataattt acaaagaaat
     2521 ataagatttt acgctatttt ttgaaatgga atttatcgta tatttctatt attttaactc
     2581 tcgagctccg caaatttctg acgatatttc attgagccaa ccagcttgct gaaaaactcc
     2641 agtttgaatt tttataaaac gaatatgtag taaatcaatt gagttaccca aactatctac
     2701 agcgtttgaa atatcaaatt gattggaatg aactatttga tcgtagtcaa taccaaaatt
     2761 gttttcggca tacccccacg taaaacgatc aacgggaaca acccattgag tactattatt
     2821 ctcataagca tcaggtaaac gtgttccgta aaaagtgatg ctatcatttt cataatattt
     2881 ccaccaccac gaagcagtat attttgtacc aaaacctgca agtaattctc ccgtatttcc
     2941 ttcgctatct ttccaagtta tattggctga atctgaaaaa gccttataat aacacaccca
     3001 ataattcctt tttgagtttt caaattgatt cccttttaat tcgtaccaga tgtcattggg
     3061 ttttccatcg ccgtttttat cttccattac ataaacaata gcaggttcag gcgaaacacc
     3121 tgccaaagca aatacttcaa aatcatatcc ttcatgattg atcacattat gatcaaagcc
     3181 agctatcaca tatccgcccc aaccacccag atacagacaa ctttttatgt tagaaggtaa
     3241 gctaataaag tttgctgcat ctgcaggttt aactgtgttg gcatgttgac caggtgcata
     3301 ttgatattca aaaacatctg taatataagc agaattggtt acttcattgt ctgatttttc
     3361 acaagaaaca attactaaga ttagaaaaat aaaaaacaat agttttaaat aatgacacca
     3421 aatattaaga gacatttttc tgtaaattaa aaaatgaaaa aaattatcga atcgttatca
     3481 ttgtcgctcc aaaaccatat tgctgaaacg aagcgtcctg aaagtaaaat tttttgtatt
     3541 gtgtttccag aagtttctga atttcttttt ttaatacgcc ttcacctttt ccatgaatga
     3601 aaacgatttt ttgacctttg ttatctttgt tcatttccag tacttccctg catttgttca
     3661 attgaagttg aagcatttca aaattactca ttcctgctgg attatctatt aattgttcaa
     3721 tatg
//
LOCUS       DAOTKS010000132         3676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_21086_length_3676_cov_4.140845, whole genome shotgun sequence.
ACCESSION   DAOTKS010000132 DAOTKS010000000
VERSION     DAOTKS010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21086_length_3676_cov_4.140845"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2762)
                     /locus_tag="P5236_03605"
     CDS             complement(<1..2762)
                     /locus_tag="P5236_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HRU63480.1"
                     /translation="MKRFKLLNTIFGWVAFAIAFVTYLTTLEPTASFWDCGEFIATAY
                     KLEVGHPPGAPFFMMTARLFSLFTSDPSKVAVMINALSALCSALTILFLFWTITHLAK
                     KILIKSEENYTLANIIIIIGAGLVGALAYAFSDTFWFSAVEAEVYAYSSLFTAVVFWL
                     ILKWEEKADEPGSDRWLLLIAYLMGLSIGVHLLNLLAIPAIVLVYYYKKYTPSTKGTF
                     LTLLVSVVLLAVVLYGIIPGSIQVACWFELFFVNTLGLPFNSGLYVYLILTIAALAWA
                     IYESYVAKNYFRMTFSILLAITLAGIPFFGAHIVLGIILIAALGIYFYHGKSKVSPHF
                     LHTSILMITLMLIGYSTYALIVIRSAANPPLDENSPDNVFALKYYLNREQYGDRPLFY
                     GATYNAPVKLRIEGNMCIPERKVGEPMYTPKPKTTPDEKDIYLKTGNKFDYKMDERFC
                     MFFPRMYSSSPNHIQAYKEWGKVKGKQITYDYCGQQKTDVCPTFFENLRFFFNYQLNF
                     MYWRYFLWNFSGRQNDLQGNGEVDKGNWITGINFIDNMLVGNQKNLPTALATNKGHNT
                     YYMLPLLLGILGIFSQIYGGKEGRRGFWTVLLLFFMTGIAIVVYLNQTPYQPRERDYA
                     YAGSFYAFSIWIGLGLIELFKTVNKYLSDISSSILVTLLSLGVPTLMAAQNWDDHDRS
                     ERYTARDFGHNYLASCRQNAIIFTNGDNDTFPLWYNQEVEGNRTDVRCCNLSYLQTDW
                     YIDQMKRQAYESDPLPISWKKEDYLSGKNEVVQVEDVINQPLQLKTVIDFILSTDSYT
                     KIDGEPFIPTKKVYLPIDPEEVLKTGTLKPERANEIIPQIDISLKNRLTKSELMVLEM
                     LKENKWERPMYFAVTVGDDYYLGLTDHFELTGLAYQILPIGVKGAGPGVNVDEMYDNV
                     MNKFRFG"
     gene            complement(2935..3507)
                     /locus_tag="P5236_03610"
     CDS             complement(2935..3507)
                     /locus_tag="P5236_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63481.1"
                     /translation="MTDEEKLSKNDEETNKNLSHQGKKKESKIKVWIKKHKSVCFLIV
                     VLIVVLLAAFIKIKFMERNFAKEKFKLRSCYEHQLDSIKSSQLVTISEVFSWAVRSEL
                     LRNNKEQINQFFLTLIKKPGFQKIQLIDPVSTRVILSTDKKEEGQIFRDAHRFIIPET
                     ISFKKDSLLQIVTPITGLNDQIGILVIECN"
BASE COUNT         1282 a          669 c          554 g         1171 t
ORIGIN      
        1 ccaaaacgga atttattcat cacattatca tacatttcgt ctacattaac gccaggtcca
       61 gcacctttaa ctccaatggg caaaatttga taagccaatc ctgtcaattc aaaatggtca
      121 gtcaatccca gataataatc atcaccaact gttacagcaa aatacattgg ccgttcccat
      181 ttgttttctt tcaacatttc aagaaccatc aattcacttt ttgtaagtct attttttaat
      241 gaaatatcta tttgtggaat aatttcattt gctctttcag gtttcagtgt gcctgttttt
      301 aaaacttctt cagggtcaat gggtaaataa accttttttg taggaataaa aggttctcca
      361 tcaatttttg tatatgagtc agtacttaaa ataaaatcta tcactgtttt caattgaaga
      421 ggttgattga tgacatcttc cacttgaaca acttcatttt tccccgataa ataatcttct
      481 tttttccacg aaatgggaag aggatcagat tcatatgcct gacgtttcat ctggtcgata
      541 taccagtctg tttgaagata actgaggttg caacatctta cgtctgttct attaccctct
      601 acttcctggt tgtaccataa gggaaaagta tcattatcgc cattcgtaaa aataatagca
      661 ttttgtcggc acgaagccaa ataattatgc ccaaaatcgc gagcagtata acgttcactc
      721 cgatcatgat catcccagtt ttgagctgcc attaaagtag gaacacctaa agacagcaag
      781 gtcaccaaaa ttgagcttga tatatcagat aaatatttgt tgactgtttt aaaaagttct
      841 attagcccca acccgatcca gatgctgaaa gcataaaatg aaccggcgta agcatagtct
      901 cgttcgcgag gctgataagg cgtttgattc aaataaacta caatggcaat acctgtcatg
      961 aaaaataata agagaactgt ccagaatcct cttctgcctt ctttacctcc gtagatttgt
     1021 gaaaaaatcc ccaaaattcc taacaataaa ggtaacatat agtaagtatt atgaccttta
     1081 ttagtagcca gtgcagttgg taaattcttt tgattcccca ccagcatgtt atcaataaaa
     1141 tttattcctg taatccaatt acctttgtcc acctctccat ttccttgtaa atcattttgt
     1201 cgcccactga aattccacag gaaataacgc caatacatga aatttaactg ataattgaaa
     1261 aagaaacgta gattttcaaa aaaagtaggg cacacatctg ttttttgttg tccacaatag
     1321 tcataagtaa tctgtttgcc ttttactttc ccccattctt tataagcttg aatatgattt
     1381 ggagaactac tgtacatacg agggaagaac atacaaaaac gttcatccat cttataatca
     1441 aatttatttc ctgtttttag ataaatatct ttttcatctg gagttgtttt aggttttggt
     1501 gtatacatgg gttctcctac ttttcgttcg ggaatgcaca tatttccttc tatacgaagt
     1561 ttaactggtg cattataagt ggcaccatag aaaagaggac gatctccata ttgttctcga
     1621 ttcaaataat attttaaggc aaaaacatta tcaggtgaat tttcatccag aggtggatta
     1681 gcagcagaac gaataactat caaagcataa gtagaataac ctatcagcat taacgtaatc
     1741 atcaaaatag aggtatgaag gaaatgaggg ctaactttgc tttttccgtg ataaaagtaa
     1801 atgcctaaag ctgctattaa aataattccc aacacaatat gagccccgaa aaatgggatc
     1861 cctgccaatg taattgccag aagaatggaa aaagtcattc taaagtaatt tttagctacg
     1921 tacgactcat atattgccca tgctaaagct gcaatagtca aaataagata gacatataaa
     1981 ccactattaa atggtaaacc gagcgtatta acaaaaaaca attcaaacca gcaagccacc
     2041 tgaatcgatc ctggtattat tccgtataat acaactgcta acaaaactac tgaaactaat
     2101 aaagttaaaa aagttccttt agtagaaggc gtgtattttt tataataata aaccaaaaca
     2161 attgctggaa ttgccagtaa atttaacagg tgtacaccaa tagacaaacc catcagataa
     2221 gctattaata agagccaccg atcagagccc ggttcatcag ctttttcctc ccactttaaa
     2281 attaaccaga atactacggc tgtaaacaat gaagaatagg catacacttc ggcttctact
     2341 gctgaaaacc agaaagtatc ggaaaaagca taagccaaag cacctactaa ccctgcacct
     2401 attatgataa taatattggc taaagtataa ttttcttcag atttgattaa aattttttta
     2461 gccagatgag taatcgtcca aaataaaaaa agaatggtca aagcactaca taacgccgac
     2521 agtgcattaa tcattactgc cacttttgat ggatcggaag taaacaagct gaaaagacga
     2581 gcagtcatca taaaaaaagg agcaccagga ggatgaccta cttctaattt ataagctgta
     2641 gcaataaatt ccccacaatc ccaaaaactg gcagttggct ccaatgttgt taaataagtt
     2701 acaaaagcaa tggcaaaagc tacccatcca aaaattgtat tcagtaactt aaaacgcttc
     2761 atttaattat tgttttaact cgtttataga aggatatgcc ttcaaagata caaagaattt
     2821 tttttaaaaa gagaatatta ttccttttta gttaaaattt agtgttcaaa aaataaaaaa
     2881 ggaaggggtt gattcaatta tattgaacga cctcttatga aaaaattcag aaaatcaatt
     2941 acattcaatt accaaaatcc ctatctgatc attaagacca gtaataggag taacaatctg
     3001 caataaagaa tcttttttga atgaaatagt ttcgggaatt atgaaacgat gagcatctct
     3061 gaaaatttga ccctcttctt ttttatcggt tgagaggata actcttgtac ttacaggatc
     3121 aattaattga attttttgaa aacctggttt ttttatcagg gtcaggaaaa attgattgat
     3181 ctgttctttg ttgttgcgaa gcaactcact tcttacagcc catgaaaaaa cctcagaaat
     3241 cgtaaccaat tgtgaacttt taatactatc cagttgatgt tcataacaag agcgtaattt
     3301 gaatttttct tttgcaaaat ttcgttccat aaacttgatt ttaataaaag ctgccagtag
     3361 caccactatc agcacaacaa tcaaaaaaca aacagattta tgttttttga tccagacttt
     3421 tattttactt tctttctttt tcccctgatg agacaaattt ttatttgtct cttcgtcgtt
     3481 ttttgataat ttttcttcgt ctgtcatagg tttaattttt taagaaaaac aacacttaaa
     3541 aaaattatat ggagaaaaaa tatgataaaa ttcaaaatta aaatgtaaca aatttaatta
     3601 aaagttgaaa gatttatttt ttaaaaatca ttcataagct cccattttga gcatattgat
     3661 ttctttttgt gtaagg
//
LOCUS       DAOTKS010000133         3667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_21154_length_3667_cov_3.654762, whole genome shotgun sequence.
ACCESSION   DAOTKS010000133 DAOTKS010000000
VERSION     DAOTKS010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21154_length_3667_cov_3.654762"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="P5236_03615"
     CDS             complement(<1..333)
                     /locus_tag="P5236_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HRU63482.1"
                     /translation="MKNICFYFEIHQPVRLKRYRFFDIGNDHYYYDDFQNEDRIRFLA
                     EQSYLPANKAIYEMIRNSNGKFRCAYSFSGIALEQLEQYAPEVIDSFIELAKTDCVEF
                     LAETYAHSL"
     gene            complement(353..1609)
                     /locus_tag="P5236_03620"
     CDS             complement(353..1609)
                     /locus_tag="P5236_03620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRU63483.1"
                     /translation="MKVLMFGWEFPPHILGGLGTASYGLTKALAMMDDIDITFVIPKK
                     RGDEDESFLKIIGACFVPIVWKENDWNYVNQRIGWIMSPDEYFKLRNGIKYDFRRIYT
                     DDLGCVEFSGIYPNNLIEEIFNYEAVASVLAHQLDFDIIHSHDWLTYPAGVFAKQISG
                     KPLVIHVHATEFDRSRGQVNPVVYDIEKRGMDMADHIITVSNLTRNIVIEKYHQDPRK
                     ITTVHNAVEPLPDADSYVKTIRRDKIVTFLGRITMQKGPEYFVEAAQRVISKTKNVRF
                     VMAGNGDMMYSMIDLVAKQRIADRFHFTGFLVGRQVYQMLADSDVYIMPSVSEPFGIS
                     PLEAMQVGTPTIISKQSGCAEILTHTIKIDYWDIDGMADAIYAIVRSPAMYKSLHELG
                     KMEVSNIKWENAAQKVKTIYESLLKK"
     gene            complement(1667..>2013)
                     /locus_tag="P5236_03625"
     CDS             complement(1667..>2013)
                     /locus_tag="P5236_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HRU63484.1"
                     /translation="SLSPKSGFYRPVYVGGMLERNRNYHNGPVWPFTFGAFATAYLKI
                     YKQSGKSFIERMLIGFEAEMKELCIGTLSELYDGNPPFKGHGGISFAMNVAEVLRVLE
                     ILKNYDNNQTSE"
     assembly_gap    2014..2113
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2114..3463)
                     /locus_tag="P5236_03630"
     CDS             complement(<2114..3463)
                     /locus_tag="P5236_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme N-terminal
                     domain-containing protein"
                     /protein_id="HRU63485.1"
                     /translation="MSYLSFDRSLLINLERSLSKEILRTNRAGTYNCTTLVDCNTRKY
                     HGQLVVPLPEIDEYNHVLLSSVDETVVQHGIEFNLGVHKYEGNNFYPNGHKYIRQFDC
                     EIIPRTIYRVGGVVLAKERLLVSFEPRVLFKYTLLEAHSPTLLRFKPFLAFRSVHELT
                     HQNNVANTSFDEVHNGIKMCLYDGYPSLYMQFSKKNIYHHYPDWYKNIEYFKEQERGF
                     EYKEDLLVPGSFELPIKKGESIIFSAGISEVSTHQLKFLWEKELKKRHPRMDMFSCLK
                     NSAQQFYKREGGKTYLLAGYPWFGARARDEFLALPGCTLSIGRMDYFDTIIETAIPEL
                     QHFMNGDQIKFKLQEIDAPDVLLWFIWAIQEYANHASINEATQRFGELIYQIIDFIQK
                     QKHPNLFLHSNGLLYVNGVDKPATWMNAVENGRPITPRTGYVVEINALWYNAIKFAAQ
                     "
BASE COUNT         1236 a          667 c          464 g         1200 t
ORIGIN      
        1 caaagaatga gcatacgttt cagccaaaaa ttcaacacaa tcggttttag ccagttcaat
       61 aaaactatca atcacttcag gtgcatattg ttctaattgt tccaaagcaa taccactaaa
      121 tgaataagca cagcgaaact taccattaga atttctaatc atttcataaa tagctttatt
      181 agcaggcaaa taagactgct cggctaaaaa gcgaatacga tcttcatttt ggaagtcatc
      241 ataataataa tgatcattcc caatatcaaa aaaacggtat cttttcaagc gaacaggttg
      301 atgaatttca aaataaaaac atatattttt cataattttt tattttactc aattattttt
      361 tcaataaact ttcataaata gttttcactt tttgagcagc attttcccat ttaatattac
      421 ttacttccat tttccctaat tcatgtaaac ttttatacat ggcaggactt ctaacaatgg
      481 cataaatagc atctgccatt ccatctatat cccaataatc aatttttata gtatgagtta
      541 atatttctgc acaaccagac tgttttgaaa taatagtagg tgtaccaact tgcatagcct
      601 ctaaaggaga aataccaaaa ggttctgata ctgagggcat tatataaaca tcactatcag
      661 ccaacatttg ataaacttga cgtcctacta agaacccagt aaaatgaaat ctatcagcga
      721 tacgttgttt agcaaccaaa tcaatcattg aatacatcat atctccatta ccagccatta
      781 caaatcgtac atttttcgtt ttactgatta ctcgttgagc agcttccaca aaatattcag
      841 gtcctttttg catagtgatt ctccccaaaa aagttacaat tttgtctctc cgaattgtct
      901 ttacataaga atcggcatct ggaagaggtt caacagcatt atgaacagtt gttatcttac
      961 gagggtcctg atggtatttt tcgatgacaa tatttcgtgt taaattactc accgtaatta
     1021 tatgatcagc catatccatt cctcgttttt caatatcata aaccacagga tttacttgtc
     1081 cacgacttct atcaaattca gtagcatgta catgaataac aagaggtttt ccactgattt
     1141 gtttggcaaa aacccctgct ggataagtaa gccaatcatg agaatgaatg atatcaaaat
     1201 ccaattgatg agccaacaca cttgcaactg cttcataatt aaatatttct tcaattaaat
     1261 tatttggata tatacctgag aattctacac agccaagatc atcagtataa atccgtctga
     1321 aatcatactt aatgccattt cttaatttaa aatattcatc gggagacatt atccagccaa
     1381 ttctttgatt tacataattc caatcatttt ccttccatac tattgggaca aaacatgctc
     1441 caataatttt taaaaaactt tcatcttcat cgcctcgttt tttaggaatg acaaaagtaa
     1501 tgtcaatatc atccatcatt gccagagctt ttgtcaatcc ataacttgct gtccccaatc
     1561 cacccagaat atgaggagga aattcccaac caaacattaa tactttcata cgccaaataa
     1621 ttgattcttt aaatgctact aaaaaaggaa aaaattttct gataaattat tcagaagttt
     1681 ggttgttatc gtaatttttt aatatttcca aaacccttaa cacttccgct acattcattg
     1741 caaaagaaat tcctccatgt cctttaaaag gtggattacc atcgtaaagt tcagataaag
     1801 ttccaataca caattctttc atttctgctt caaatccaat cagcattcgt tcgataaaag
     1861 atttaccact ttgtttatag atttttaaat aagcagtagc aaaagcacca aatgtaaatg
     1921 gccatacagg tccgttatga taattcctat tacgttccaa cattcctcct acataaacag
     1981 gtcgataaaa tccactttta ggacttaaac ttcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnttgagct gcaaatttta ttgcattgta ccataaagca ttaatttcaa
     2161 ccacataacc tgttctcggc gtaatgggac gaccattttc aacagcattc atccatgttg
     2221 caggcttatc aacaccattt acgtacaata atccattgct atgtaaaaac aaattaggat
     2281 gcttttgttt ttgtataaaa tcaatgattt gatatattaa ttccccgaat ctttgagtag
     2341 cttcatttat tgaagcatgg ttagcatatt cctgaatagc ccaaataaac cacaataata
     2401 catcaggagc atctatttcc tgtaatttga atttaatttg atctccattc ataaaatgtt
     2461 gtaattcagg aattgcagtt tcaataatag tatcgaaata atccattcgt ccgatgctca
     2521 atgtgcaacc aggtaaagct aaaaattcat cacgggctct tgcaccaaac cacgggtaac
     2581 ctgccaataa ataagttttt cccccttccc gtttataaaa ttgttgagca gagtttttta
     2641 aacaactaaa catatccatt cgtggatgac gtttttttaa ttctttttcc caaagaaatt
     2701 ttaattgatg agtagaaact tctgaaatac ctgctgaaaa aataatggat tcacctttct
     2761 tgatgggtaa ttcaaaactg ccgggaacca acaaatcttc cttatattca aagcctctct
     2821 cctgctcttt gaaatattca atatttttat accaatcagg ataatggtga taaatatttt
     2881 ttttagaaaa ttgcatatac aaagacggat aaccgtcata tagacacatt ttaattccat
     2941 tatgaacttc atcaaacgaa gtgtttgcga cattgttttg atgagtaagc tcatgaacac
     3001 tccgaaatgc caaaaaaggt ttaaatctga gtaaagttgg cgaatgagct tccagcagag
     3061 tatatttaaa taatacacga ggttcaaatg agacaagcaa tctttcttta gctaaaacaa
     3121 ctcctcctac tctataaata gtacgaggaa ttatttcaca atcaaattga cgaatgtatt
     3181 tatgaccatt tggataaaaa ttatttcctt catacttatg aacccccaaa ttaaattcta
     3241 ttccatgttg taccactgtc tcatcgactg aagaaagaag tacatgatta tattcatcta
     3301 tttctggcaa cggaacaaca agttgtccat gatatttacg agtattacaa tcaacaagag
     3361 tagtacaatt ataagtaccg gctcgattgg tgcgaagaat ttctttggat aatgaccttt
     3421 caaggttaat taataaagac cgatcaaaac taagataact cattatattt catatttata
     3481 agtacctaat tagctaattt tttataaaaa aagcgatatt caaaagaatt aaatcttagc
     3541 atcacttttt tattaaatag atgtttcgca atttatttat ttttttttaa ctaaaaaact
     3601 tttttggtat taatttatga tttaaaatta ttatttttga aattattttt tgcaaaaaat
     3661 aaaaatt
//
LOCUS       DAOTKS010000134         3641 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_21329_length_3641_cov_4.738706, whole genome shotgun sequence.
ACCESSION   DAOTKS010000134 DAOTKS010000000
VERSION     DAOTKS010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21329_length_3641_cov_4.738706"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="P5236_03635"
     CDS             complement(<1..267)
                     /locus_tag="P5236_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRU63486.1"
                     /translation="MAKQNFTYEDVLNDLRQKIYFPVYFLMGEEPYYIDLVSDYIQNN
                     ILTETEKDFDQTIFYGKDVNIPTIINVAKRFPMVAKHQVLIVKEA"
     gene            complement(284..1057)
                     /locus_tag="P5236_03640"
     CDS             complement(284..1057)
                     /locus_tag="P5236_03640"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HRU63487.1"
                     /translation="MKTKKEIVENWLPRYTKRPLEDFTNHILLVNFNIYLELFANYYQ
                     VPIIGRNGNMPNASANGITMINFGLGSPNAALIMDILSAVQPKAVLFLGKCGGLRDKN
                     KEGDYILPIAAIRGEGTSNDYFPPEIPALPAFSLQRAVSSNIRDFGKDYWTGTVYTTN
                     RRVWEHDEKFKEYLRSTKAMAIDMETATLFSSGFYNGIPTGALLLVSDMPMHEEGIKT
                     IESDNRVNANYVEEHLKIGIASLVSLITNSRTVKHLRFE"
     gene            1145..1612
                     /locus_tag="P5236_03645"
     CDS             1145..1612
                     /locus_tag="P5236_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HRU63488.1"
                     /translation="MLQLNLPAYNFRLKKERDTYWIFDNIRKKFVKLTPEEWVRQNFI
                     RFFTEEKHFPASRLSVEEEIVWNGMKKRCDAIFYNEYAEPALILEFKAPQITISQLVF
                     DQVAVYNVKLNVNFFIISNGIEHFFCKVNVKNSQFEISREIPDYKIFIELNRQ"
     gene            complement(1623..2537)
                     /locus_tag="P5236_03650"
     CDS             complement(1623..2537)
                     /locus_tag="P5236_03650"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HRU63489.1"
                     /translation="MVKLNVDIGKLQLKNPVMTASGTFGYGIEYADFLDLSRIGSIIV
                     KSITLRNREGNPYPRMKETASGMLNAVGLQNKGVKYFVENIYPLLKNIDTCIIANIAG
                     STIEEYVQIAEILNELDKISALELNISCPNVKEGGMLFGNSSIAANQVVTAVRRVYSK
                     ELIVKLSPNVTSISEIAIAVENAGADAISLINTLVGMAIDIETRKPCLSTITGGLSGP
                     AIKPVAVRMVWEAAHAVKIPIIGIGGIMNAADAIEFLLAGATAIEVGTANFIDPTVTI
                     KIIEGIEQYLIQHGFDNVYEIIGAMENK"
     gene            complement(2531..3295)
                     /locus_tag="P5236_03655"
     CDS             complement(2531..3295)
                     /locus_tag="P5236_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HRU63490.1"
                     /translation="MTKCILDFRVRKNIHLTTEHSLLILSVDQPLPEILPGQFAQVRI
                     DNSSVFLRRPFSIHYVDKKKNELWLLIQVVGEGSRKLYHINKNEILNLILPLGNSFSL
                     PKNHKDKILIIGGGTGIAPLLFLGDYLHKNGFCPNFLFGARNKNYLIPLKKFETYGNI
                     FITTEDGSTGEKGLVTCHSILKNHFDMIYCCGPTSMMKAVASYAEKNNIPCEVSLENT
                     MACGFGVCLCCVTDTTEGRVCVCTEGPVFNITKLKW"
     gene            complement(3440..>3641)
                     /locus_tag="P5236_03660"
     CDS             complement(3440..>3641)
                     /locus_tag="P5236_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63491.1"
                     /translation="NYEEKNLNVNSPPHKIKSELDKSPLNKSFATSEQISSNIQKIEK
                     SVKKIIVYFSDNTFQEFESKET"
BASE COUNT         1225 a          616 c          520 g         1280 t
ORIGIN      
        1 agcttctttt acaatcaaca cttgatgttt tgctaccatt ggaaatcgct tggcaacatt
       61 aataatagta ggtatattta catctttccc ataaaaaatg gtttgatcaa aatctttttc
      121 tgtttcagtc aaaatattgt tttgaatata atccgatacc aaatcaatat agtaaggctc
      181 ttctcccatt aaaaaataaa caggaaaata aattttctgc cgcaaatcat ttaaaacatc
      241 ttcgtaagta aaattttgct tagccatttt taacagttat tttttattca aatcttaaat
      301 gctttacagt tcgactattg gtaatcaagg aaactaatga agcaatgcca attttcaaat
      361 gttcttctac ataattggca ttcactctat tatcgctttc aatggttttt atcccttctt
      421 catgcatagg catatcggaa actaacaata aagctcctgt tggaattcca ttataaaatc
      481 ctgaggaaaa caacgtcgct gtttccatat ctattgccat agctttggta gaacgaagat
      541 attctttaaa tttctcatca tgttcccata ctcttctatt agttgtgtaa acggttcctg
      601 tccagtaatc tttcccaaaa tcacgaatat ttgaagaaac cgcacgctga aggctaaaag
      661 caggcaaggc tggaatttct ggcggaaaat aatcgttcga agttccttca cctcgaatag
      721 cagcaatggg taaaatataa tcgccttctt tatttttatc tcttaaacca ccacatttac
      781 ctaaaaacaa aacagctttt ggttgtactg ctgacaaaat atccataata agagcagcat
      841 ttggactgcc caaaccaaaa ttaatcatcg ttataccatt cgctgaggca ttgggcatat
      901 ttccatttct tccaataata ggaacttgat aataattggc aaacaattcc agataaatat
      961 taaaatttac cagcaaaata tgattggtaa aatcttccaa tggtcgtttt gtatatcggg
     1021 gtaaccagtt ttctactatt tcttttttcg ttttcatctt gtgtcgtttt ttgatacatt
     1081 tttgtatatt cgtattttat ttctggtagg aaatagatcg agatatagag caaaattaca
     1141 aaaaatgtta caattaaatt tgccagctta caattttcgt ttaaaaaaag aacgagatac
     1201 ttactggata tttgataata tccgaaaaaa atttgtaaaa ctcactcctg aagaatgggt
     1261 aagacaaaat tttattcgtt tttttaccga agaaaaacat ttccctgcca gtcgtttgtc
     1321 cgttgaagaa gaaattgttt ggaatggaat gaaaaaacgt tgcgatgcta ttttttataa
     1381 tgaatatgct gaacctgcct tgatattaga atttaaagct ccacaaataa ctatctctca
     1441 attagttttt gatcaagtgg ctgtttataa tgtaaaatta aatgtaaatt tttttatcat
     1501 aagcaatgga atagaacatt ttttctgtaa agttaatgta aaaaattcgc agtttgagat
     1561 ttcaagagaa atacctgatt ataaaatatt tatcgaatta aatagacaat aaaaataaat
     1621 ttttatttat tttccattgc cccaataatt tcataaacat tgtcaaaacc atgttgaatc
     1681 aaatattgct caattccctc gattatttta atggtcactg ttggatcaat aaaattagcc
     1741 gttcctactt ctattgcagt agctccagcc aataaaaatt caatagcatc ggcagcattc
     1801 ataattcctc caataccaat aattgggatt ttaacggcat gagctgcttc ccaaaccatt
     1861 cgaacagcta cgggtttgat tgcaggacca gacaagcctc ccgtaatggt ggaaaggcaa
     1921 ggttttctgg tttctatatc aattgccatt cctaccaatg tgttaattaa tgaaatggca
     1981 tcagcacctg cattttctac ggcaattgct atctctgaaa tactcgttac atttggcgaa
     2041 agctttacaa ttagttcttt actgtatacc cttcgcactg ctgtaactac ttgattagca
     2101 gctattgatg agtttccaaa aagcattcct ccttccttca catttggaca tgaaatattc
     2161 aattctaagg cagaaatttt atcaagctca ttcaatattt cagcaatttg aacgtattct
     2221 tcaatggttg aacctgcaat gttggcaata atacacgtat ctatattctt taaaagagga
     2281 taaatgtttt cgacaaaata ttttactccc ttattttgca gtcctacagc atttagcatg
     2341 cctgacgcag tttctttcat gcgaggatat ggattccctt ctctattgcg aagtgtaata
     2401 ctttttacaa taatactacc tattcgtgat aaatcaagga agtctgcata ctctattcca
     2461 tagccaaaag tacctgaagc tgtcatcaca ggatttttca actgtaattt tcctatatcg
     2521 acatttagtt ttaccatttt aattttgtaa tattaaaaac cggaccttct gtacaaacac
     2581 aaactcttcc ctctgtggta tctgttacgc aacataaaca aactccaaat ccgcaagcca
     2641 tcgtattttc taaggatact tcacacggga tgttattttt ttcagcataa gaagctacag
     2701 ctttcatcat ggaggttgga ccacaacaat aaatcatatc aaaatgattt tttaaaatag
     2761 aatggcaagt taccaaacct ttttctcccg ttgagccatc ttcggtagta ataaagatat
     2821 ttccgtaagt ttcaaatttt tttaaaggaa ttaaatagtt tttatttcga gctccaaata
     2881 aaaaatttgg gcaaaaacca tttttatgta aataatcacc caaaaaaagt aaaggagcta
     2941 tacctgttcc gcctccaata attaaaattt tatctttatg attttttggc aagctaaaag
     3001 aattacccaa aggtaatatt aaatttaaaa tttcattttt attgatatga tacaattttc
     3061 tgcttccttc tcctaccact tgaattaata accataattc attttttttc ttatcaacat
     3121 aatgaataga aaaaggacgg cgtaaaaata cggaagaatt atcaattctt acttgagcaa
     3181 attgtccagg caaaatctct ggaagtggct gatcaacaga aagaattaac agggaatgtt
     3241 cggttgttag atggatattt tttcttactc taaagtcaag tatgcatttt gtcattatac
     3301 ttgataattt taaaaaatca atttaaaaaa ggcatatttg attataattg attacaaatt
     3361 tagagattaa aattcaattg acaagaagga atgttgaatt gtttttttag aataaataag
     3421 tttaaatagg taaaaaagac taagtttctt ttgattcaaa ttcttgaaag gtattatcac
     3481 taaaatagac aataattttt ttaactgatt tttcaatctt ttgaatatta gatgaaattt
     3541 gttctgaggt tgcaaatgac ttgttcaatg gagatttatc aagttcactt ttaattttat
     3601 gaggaggaga atttacattt aaattttttt cttcataatt a
//
LOCUS       DAOTKS010000135         3633 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_21376_length_3633_cov_4.198435, whole genome shotgun sequence.
ACCESSION   DAOTKS010000135 DAOTKS010000000
VERSION     DAOTKS010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21376_length_3633_cov_4.198435"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /gene="mscL"
                     /locus_tag="P5236_03665"
     CDS             complement(<1..391)
                     /gene="mscL"
                     /locus_tag="P5236_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HRU63492.1"
                     /translation="MKELLAELKAFLMRGNVVDMAVGIVIGAAFGKVVTSFVNDILMP
                     PISLLLGSTNFSDLKIVLKNAVVEGTNVVAPAITWNYGNFIQIIIDFLIVGTAIFFLI
                     KGMNQLSRKKEEAPAPAPEPTNEEKLLA"
     gene            1051..2247
                     /locus_tag="P5236_03670"
     CDS             1051..2247
                     /locus_tag="P5236_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HRU63493.1"
                     /translation="MFQNKQVAKDIFYVGVNDRQKAKFENIIPLPFGVSYNSYLIIDD
                     KTALMDTVDFHQGEIFVDKIQSQLQGRKLDYLIINHMEPDHSGSIRLIRKYFPEVILV
                     GNSRTLKMIEGFYGETSCVKEIHDEEILSLGTHELQFFLVPMVHWPETMMTFDRTEKV
                     LFSGDVFGCFGALDGAVVDTEMNVGKYWDEMIRYYANIIGKYGSPVQKAIQKLSGVPF
                     ETICTTHGPVWKENIQKTINIYDQLSKYEGGNGIVIVYASMYGNTELMAETIAQGVAE
                     AGVKEITIHNASTTDASFILRDIFKYKGLIIGSPTYNNELFPEIEALLQKIELRQVKN
                     RIFGCFGSYTWAGAAVDRLSLFAEKMKWIMVDKGIEEKQALKDNQYKACLQLGLKMGE
                     MLIQSF"
     gene            2338..2568
                     /locus_tag="P5236_03675"
     CDS             2338..2568
                     /locus_tag="P5236_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HRU63494.1"
                     /translation="MKKNNPSELSKINYLLIAIGFVIIVIGFFLMTGSSTEITFNPDI
                     FSFRRITLAPMISLFGFLFIIVAILYQPKKKE"
     gene            2569..3453
                     /locus_tag="P5236_03680"
     CDS             2569..3453
                     /locus_tag="P5236_03680"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRU63495.1"
                     /translation="MSFFQAILIAIVEGLTEFLPVSSTGHMIIIQSLLGIESTDFVKV
                     FTVNIQFGAILSVIVLYWKRFFKLNPLPALDKTFIESKKDFAKWKVYFQRFLYKYDFY
                     WKLLIGFLPTGIIGFLASDYIDTLLGEVWVVALMLLLGGILMIFIDQWLKYPSSNQEI
                     SWKRALEIGFCQCIAMIPGVSRSMATIVGGMATKLNRKNAAEFSFFLAVPTMAAASIF
                     KLIELLHTSNGSQVLTQNLSLLLVGNIVAFVVAMAAIKFFIDFLTKHGFKAFGYYRII
                     VGAFLLILIYLGIDLKIV"
     gene            3453..>3633
                     /locus_tag="P5236_03685"
     CDS             3453..>3633
                     /locus_tag="P5236_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase"
                     /protein_id="HRU63496.1"
                     /translation="MDVIAGEVFYVDKPLHWTSFNVVDRIRKKITKKLKIKNIKVGHA
                     GTLDPLATGVMIICTG"
BASE COUNT         1220 a          545 c          648 g         1220 t
ORIGIN      
        1 ctgcgagcaa cttttcttca ttagttggtt ctggagcagg tgctggtgct tcttcctttt
       61 ttcgacttaa ttgattcatg cctttaatca aaaagaaaat agctgtccca acaataagaa
      121 aatcaataat aatttggata aaattcccat aattccaggt aatagctggt gcaacaacat
      181 ttgtaccttc tactaccgca ttctttaata ctattttcaa atcagagaaa tttgtacttc
      241 caagaagtag cgaaattggc ggcataagaa tatcgttaac aaaagaagtt actactttac
      301 caaaagcagc accaattaca ataccaactg ccatgtcaac aacattacca cgcattaaaa
      361 atgcttttaa ttctgctaat agttctttca taatgataat aatttaatgt taattttttt
      421 atttgcaaat aaaagaaaaa aaccaatgtg ttcaataatt aaagaaagaa ttttaagacg
      481 tttatagtaa atagatttgc aaaaatgggg aaggatagaa tttgtccgac aagtaaaaaa
      541 ctgacaaatt agcgagatgc aacgtgcgag gtgcgagaat gcaaattccg agtttggtgt
      601 tttgaagtag aaatgctttg tgaagcgtct aacgaggtac gaagttcaag ttttgagttt
      661 tgagcgaatg aagaaaaaat ttctgtatcc cattgttcga cgtactctcc aggctacgtc
      721 gaaataaaaa taaaatctct tgattttaac cgctgatata attaaacgct ttgctaaacc
      781 aatatttgtc ataaaaaacc ttattaaaaa cctataacct tcataacaag gaatgttccc
      841 tatagcttac aaccttatta tcttatttat caggtcgttt ttcttttctc tgaatgtaat
      901 tagcgacgcc atttagaatc attacctcca atgtatatca aaaaaggtgc gtagcattga
      961 tatttttgta gccactttgt aaaatttgtt ttatctttgt agtactattt ttacatatta
     1021 tcaaaatcct gtaaaaaagc ttcaacaatg atatttcaga ataaacaagt agcaaaagat
     1081 attttttacg ttggagtaaa tgatcgccag aaagcaaaat ttgaaaatat aattccgtta
     1141 ccttttggag tttcgtataa ttcttattta attattgatg ataaaacggc cttaatggat
     1201 actgttgatt ttcatcaagg tgaaatattc gttgataaga ttcaatctca attgcaaggt
     1261 agaaaacttg attatttaat tatcaaccac atggagcccg atcattcagg ttctattcgt
     1321 ttgattcgca aatatttccc agaagtcata ttggttggta attcaaggac attgaaaatg
     1381 atagaaggat tttatgggga aacctcttgc gtgaaggaaa ttcatgacga agaaatacta
     1441 tcattaggca ctcatgagct tcagtttttc cttgttccaa tggttcactg gcccgaaaca
     1501 atgatgacgt ttgaccgaac agaaaaagtt ttattttctg gcgatgtttt tggttgtttt
     1561 ggtgctttag atggagctgt ggttgatact gaaatgaatg tagggaaata ttgggatgaa
     1621 atgatacgtt attatgcaaa tattattggt aaatatggtt ctcctgttca aaaagctatt
     1681 cagaaattaa gcggagttcc tttcgaaaca atttgcacga cgcatggacc tgtttggaaa
     1741 gaaaatattc agaaaacgat aaatatttat gaccaattaa gtaaatatga aggggggaac
     1801 gggattgtaa tagtatatgc ttcaatgtat gggaatactg aactaatggc tgaaactatt
     1861 gctcaaggtg tggcagaagc tggagtaaaa gaaattacca ttcataacgc ttccacaact
     1921 gatgcgtcat ttattttaag ggatatattt aaatacaagg gattgatcat aggaagtcct
     1981 acttataaca acgaactttt tcctgaaatt gaagctttgc ttcaaaaaat agagcttcgt
     2041 caagttaaaa accgtatttt tggttgtttt ggttcttata catgggcagg agcagcagta
     2101 gatcgtttga gccttttcgc tgaaaaaatg aaatggataa tggttgataa gggaatagaa
     2161 gaaaaacagg ctttgaaaga caatcaatat aaagcttgcc tacagttagg gctcaaaatg
     2221 ggtgaaatgt taatacaatc tttttaacaa gaaaaaaagg aaaataattt tttaaaatat
     2281 tctttagata aaggaaattc atatcatttt tttaatcttt tatcaaatta aaaagaaatg
     2341 aaaaaaaata atccatcaga acttagtaaa attaattatc tccttattgc aataggtttt
     2401 gtcattattg taattggttt tttccttatg acaggttctt ctaccgaaat tacgtttaat
     2461 cctgatattt ttagttttag aagaataaca ctcgcaccca tgatttctct ttttggtttc
     2521 ttattcatta ttgtagccat tctgtatcaa ccaaaaaaga aagagtaaat gagttttttt
     2581 caggccattt taatagccat tgtcgaagga ctgacagaat ttttgccggt ttcttctaca
     2641 gggcacatga ttattatcca aagccttttg ggaattgaaa gtacagactt cgtaaaagtt
     2701 tttactgtca acattcagtt tggagccatt ctgtcggtta tagtacttta ctggaaacgt
     2761 ttttttaagc tcaaccctct tccggctctt gataaaacat ttatcgaatc aaaaaaagat
     2821 tttgccaaat ggaaagttta ttttcaacgc tttttatata aatacgattt ttattggaaa
     2881 cttttaattg gttttttgcc aacaggtata attggattct tggcaagtga ttatatcgat
     2941 acattattgg gtgaagtatg ggtagtggct ttgatgctct tgctgggtgg aatattaatg
     3001 atatttatcg atcaatggct gaaatatcct tcatccaatc aggaaataag ctggaaacgt
     3061 gcgctggaaa tcggattctg tcaatgcatt gctatgattc caggtgtttc ccgttctatg
     3121 gcaacaattg taggcggaat ggcaacaaaa ctcaatcgca aaaatgcagc agaattttct
     3181 tttttccttg ctgttcctac tatggctgct gcttccattt tcaaactaat cgaattatta
     3241 cacaccagca atggaagtca agtactgaca caaaatttat ctcttttact tgttgggaat
     3301 atagtagctt ttgtagtagc aatggcagct attaaatttt ttattgattt tctaacaaaa
     3361 catggtttta aagcgtttgg atattatcgt atcatagtag gtgctttcct cttaatatta
     3421 atttatttgg gaatcgattt aaaaatagtt taatggacgt tattgcagga gaagttttct
     3481 atgtagataa gccactgcat tggacatcat tcaatgtggt ggaccgaata cgaaagaaaa
     3541 taacaaaaaa actaaaaatt aaaaatataa aagtagggca tgcaggtaca cttgatccct
     3601 tggcaacagg agtgatgata atttgtacag gca
//
LOCUS       DAOTKS010000136         3607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_21551_length_3607_cov_3.995214, whole genome shotgun sequence.
ACCESSION   DAOTKS010000136 DAOTKS010000000
VERSION     DAOTKS010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21551_length_3607_cov_3.995214"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..1166
                     /gene="mnmA"
                     /locus_tag="P5236_03690"
     CDS             105..1166
                     /gene="mnmA"
                     /locus_tag="P5236_03690"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRU63497.1"
                     /translation="MEIAVLLSGGVDSSVTVHLLKEQGYSPHLFYIKIGKSEDEFLHC
                     SSEEDIEIATLIARKYGCQLEIIDLHKDYWENVVVYTINKVRQGLTPNPDVMCNKLIK
                     FGVFEQKVGKNFDKTATGHYATITEQNNKIFLSTAKDPIKDQTDFLAQIDYLQVSKLM
                     FPIGHLMKNEVRKIAQQAGLPSAKRHDSQGICFLGKINYNDFIRSYLGEKKGPIVELE
                     TGKILGKHKGYWFHTIGQRKGLELSGGPWYVTQKDIDANIIYVSKGYDTEAQYGYEFT
                     MKDFHFITENPWENTENEIAITFKIRHTPEFTKGTIKQTPQGYHVVSSEKLQGIAPGQ
                     FGVIYDVASKICIGSGEIQ"
     gene            complement(2080..3607)
                     /gene="rpsA"
                     /locus_tag="P5236_03695"
                     /pseudo
     CDS             complement(2080..3607)
                     /gene="rpsA"
                     /locus_tag="P5236_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005775782.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S1"
BASE COUNT         1101 a          659 c          541 g         1306 t
ORIGIN      
        1 aaaaaattaa atttcttaac accaaatgaa tttttattac gaaatttgtc taacaaaaaa
       61 gttgcatttg tgacttgaat tcagcaaaat agaaataaaa taaaatggaa attgctgttt
      121 tattgtcggg aggagttgat agttcggtga cagtacattt gttgaaagaa caaggttatt
      181 ctccccattt attttatata aaaattggaa aaagcgagga tgaatttttg cattgttctt
      241 cagaagaaga tattgaaatt gcaacattaa ttgcacgtaa atatgggtgc caattggaaa
      301 taattgatct tcacaaagat tactgggaaa atgttgtggt ttatacgatt aataaggtaa
      361 gacaaggctt aacccccaat ccagacgtaa tgtgcaacaa attaattaaa tttggagttt
      421 ttgaacaaaa agttggtaag aacttcgata aaactgctac aggccactat gctaccatta
      481 ctgaacaaaa caataaaata ttcttatcca cagctaaaga ccctataaaa gatcaaactg
      541 atttcttagc tcagattgat tatttacaag tatcaaaatt aatgtttcct attggtcatt
      601 taatgaaaaa tgaagtccgt aaaattgctc aacaagctgg actaccaagt gcaaaacgtc
      661 atgatagtca aggaatttgt ttccttggga aaattaatta taatgatttt attcgttctt
      721 atcttggtga aaaaaaaggt ccaattgtag aattggaaac aggaaaaatt ttaggaaaac
      781 acaaaggata ttggtttcac accataggtc aacgaaaagg gttggaactt tcgggaggtc
      841 catggtatgt cacccaaaaa gacattgatg ccaacatcat ttacgtatca aagggttatg
      901 acacggaagc tcaatatggt tatgaattta ctatgaaaga ctttcatttc atcactgaaa
      961 acccatggga aaacactgaa aatgaaatag ccattacttt caaaatcaga catacacctg
     1021 aatttactaa gggaactata aaacaaacac cacaaggata tcatgtcgtt tcaagcgaaa
     1081 aattacaagg tattgctcct ggtcagtttg gagtaatcta cgacgtagcc tccaaaattt
     1141 gtattggtag cggtgaaata caataatccc ttgactttgt tactttaaat ttataagtag
     1201 ttgattatca aatatatatg ctttacataa gtactacctt ttgtatttag tcggaggtta
     1261 agtcatggta tttcaaaaag tatatttgaa atttttaaac ccactacatg atatattttt
     1321 ttaacttctt gactaattaa agaaaagact ccatattgtt tgcctggccg gatgtctttt
     1381 aaaatactta attagatttg ttaatatctg tcttgccttt caattgaaaa gattcctttt
     1441 aaagacgtat acgataatta tataaattta aacccaaagt aagctttcaa aaatagaaaa
     1501 tttcattttt tcagatcgaa ttgctttata tcctgcttat tataaataat taagccctaa
     1561 ttttttctaa aatatttcta acaatacgca ttagaaaatt ttgcacctta tcatctgatt
     1621 tacaattaac tgtaactgca tttctttcta atgctgaatc tgggtttaat gacaaatttt
     1681 ggttaaagaa aaggttgcct ttttgaagca accttttcat ttttatatga tagaaaatct
     1741 ttattcctca gaagaagatt cttttttctt ttcttctgca ttttcattct caatttcatt
     1801 tttcgtttct tccttttcct tttcattttc aggagaattt tcatctgcaa ccacttttgt
     1861 ctgaggcttt tcttttttcc gagtttcttt ttcttttgtt actttctcag cttcaactat
     1921 tttcaactct tctacaactt cttcctgttt ttctttctga gtagtttctt cttctactga
     1981 aagttcttca gccttagctt tttcctttga agttttagta gttttggttt ttttagcttt
     2041 ggttgctttt ttttcttcct gttcagcttg tttggttgct tcttcattat ccagttgatc
     2101 tttcaattca gccagtgact gaatatctcc cagcgtggtt ttttctatcg aaggtgtagt
     2161 agctacttct tttttcttag aacgtttggt aagcgttttc tttgtagttt catcttctgt
     2221 ttcggcaact ttagcctttt cttctcgttt caaatcttca taaacgcgtg tatgggaaag
     2281 aacaatcttt ttcgcatcct tattaaactc aattactttg aatggcaact tttcatctat
     2341 ttgggcttgc gtaccatctt ctttaattaa atgtttcgtt gcagcaaatc cttccacacc
     2401 ataaggcaag gcaataatgg ctcctttatc caggaattct accacaatgc cttcatggac
     2461 actacctact gtaaaaatag tttccaatac ttcccatgga ttttcttcca gttgcttgtg
     2521 tcccaaactt aaacgacgat tttctttgtc gatttccaga acaacaacct ctatatcact
     2581 tccaatttgt gtaaattcag aagggtgttt aacttttttc gtccaagata aatcagaaat
     2641 atgaattaat ccatcgacac cttcttctat ttcaacaaaa acgccaaaat tggtaaaatt
     2701 acgaaccttt gcaacatgct tgcttccgac gggataacgt tcttcgatat tttcccacgg
     2761 atcggatttt aattgtttga tacccagaga cattttccgc tcttcgcgat caagtgtcaa
     2821 gataatagct tctatttcat cacccacttt caggaagtct tgtgctgaac gaagatgctg
     2881 cgaccagctc atttccgaca catgaattaa accttctact cctggagcaa tttcgacaaa
     2941 agcaccatag tcagttaata ccactacttt acctttaact ttatcgccta ttttcaaatt
     3001 aggatccagt gaatcccacg gatgaggtgt aagctgtttt aagcctaaag caattcgctt
     3061 tttctcttcg tcaaaatcca gaataactac atttagcttt tgatccagtg aaacgatttc
     3121 gcttgggtga ttgattcgtc cccatgacaa atctgtaata tgaattaaac catctactcc
     3181 gcccagatca ataaatacac cataagacgt gatatttttt accgtgcctt caagaatttg
     3241 tcctttttct aatttactga taatttcctt tttctgttgt tcaagttctt cttcgataat
     3301 agctttatgg gaaactacca catttcggaa ttcttggtta atttttacta ctttgaattc
     3361 catagtttta tttacaaaag catcataatc acgaattggt tttacgtcaa tttgtgaacc
     3421 tggtaggaat gcttctattc caaaaacgtc aacaatcatt ccgcctttgg tgcggcactt
     3481 aatatatcct tttataattt cttgagattc aagtgcagca ttaacacgtt cccatgcacg
     3541 acttacacga gcttgtatat gtgaaaggac caattgtcct tttttatctt cttgacttta
     3601 aatatat
//
LOCUS       DAOTKS010000137         3598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_21606_length_3598_cov_3.744285, whole genome shotgun sequence.
ACCESSION   DAOTKS010000137 DAOTKS010000000
VERSION     DAOTKS010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21606_length_3598_cov_3.744285"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="P5236_03700"
     CDS             complement(<1..986)
                     /locus_tag="P5236_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63498.1"
                     /translation="MKKGELIFSVFVLLSFVCGAGVFLTSCDSNNDVSSPDNGNSSNS
                     KEVVLKSDITGTRHLVSDSVYILQGQINVSGILNIDPGTIIKGDKVTKGCLVILPGGK
                     INAIGTPDKPIVFTSRMEPGLRAAGDWGGVIIVGRAPVNQSNPTIEGLSKAIFYGGGT
                     DPNDNSGTLRYVRIEFAGIALQPDNEINGLTFCAVGRGTTVDHIQVSYSGDDSYEWFG
                     GTVNAKYLIAYCGLDDDYDIDFGYSGKVQFALGVRNPRVADVSTSNGFESDNDGNGSA
                     FQPKTKAVFCNVTLIGPWKTSADKNVNAMFGAGMHIRRNSSLSVFNSVFTGW"
     gene            complement(1140..>3598)
                     /locus_tag="P5236_03705"
     CDS             complement(1140..>3598)
                     /locus_tag="P5236_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRU63499.1"
                     /translation="SVQVVAQPRTDTELSLLREVKSSLQIVNGISSQQISKTLDTDAS
                     EVIKRVPGITVQENRFVIVRGLNQRYNNVWLNNAPTPSSEIDVKAFSFDNVPSNMIDH
                     IITYKSASPELPAEATGAFIKIFTKNIPEGNFLNIEYSVGYNNQSTFKNIYHLPSKPL
                     DIIGLGSKARELPVNFPSDLNLLPVTVQDAYALQLNRNWVAQSYKAFPAQKFQFSIGK
                     KWNLNHGTKIGNITSLSYNASNQVRDNMTNNMYEQFDAVNGVPVYLYRYTADIYATNF
                     RWGLMHNWGYQTTTGTRIEFKNLLNQIGEDKTSNTFGWNNYRQGNFKYFSNQYSSRTI
                     YSGQLSGNHQLQNSDQKKLDWNVDFSYANRLEPDRQNWSMKEAVEGTYIYVLPDVPSI
                     NELGRLFTTTHEYLSGANINFENTIKIGKILPTYKVGALTKYKTRNFSERSIAYRKEY
                     GPFSDEQINSIPFEDLFTAPYLGHGKVISVDEQTNVANTYKAKNLLAAGYFSITIPVE
                     KFILSGGVRAEYNRLLLNGYYDASSPVTVKNPAFRFFPSLNTSFNFTNKSLLRLAYAY
                     TINRPEFREISPFCYYDFTEKTSVRGNPDLKDATIHNVDLRFENYPSPNETFTLALFY
                     KNFIHPIEMVNIGVGNEFSFKNAQSAVDYGIELELKKSLEKWLKNFSLNLNASYIFSQ
                     VHFSDKQNERNRPLQGQSPYVVNAALFYQNDKAGINSSLVYNLIGKRILVAAQINQGE
                     VVIPDIYEMPRNVLDFSFNKKIGQKVELKFGVKDLLAQNFRTQQTYEYTKNGKKKSSV
                     LNNQVYNLGTIFLLGINLKF"
BASE COUNT         1169 a          686 c          590 g         1153 t
ORIGIN      
        1 ttccaacctg taaacacaga attgaacact gaaagtgaac tgttgcgacg aatatgcata
       61 cctgcgccaa acatggcatt aacattttta tcagcagaag ttttccacgg accaattaat
      121 gttacattac aaaatacagc tttcgttttc ggctgaaaag cactaccatt gccatcattg
      181 tccgattcaa atccatttga agtagaaaca tcagccacac gaggattgcg aacgccaaga
      241 gcaaactgaa cttttcccga ataaccaaaa tcaatatcat agtcatcatc caatccgcaa
      301 taagcaatta aatatttggc attgactgtt cctccaaacc actcgtaaga atcgtctcca
      361 ctataagaaa cttgaatatg atcaaccgtc gttcctcttc caactgcaca aaatgttaag
      421 ccattgattt cattatcagg ttgtagagca atacccgcaa attcgattct tacataacga
      481 agtgttccgg aattgtcatt cggatccgtg ccaccaccgt agaaaattgc ttttgataga
      541 ccttcaatag taggattaga ctgattgacg ggagcacgtc ctacaataat cacacccccc
      601 caatcgccag ctgcacgaag tcctggttcc attctcgaag taaaaacaat aggtttatca
      661 ggtgtaccaa ttgcattaat tttcccgcca ggaagaatga ccagacaacc tttcgtcacc
      721 ttatcgcctt taataatagt tcctggatca atattcagaa taccactcac attgatttgt
      781 ccctgcaaaa tataaacaga atcactcacc agatgacgtg ttcctgtaat gtccgattta
      841 agcactactt ctttgctatt cgaagaattt ccattgtcag gtgaagaaac atcattgtta
      901 ctatcgcatg aagttaaaaa aacacctgca ccacaaacaa aagataaaag aacaaaaaca
      961 ctaaaaatta attccccttt tttcattttc tgttggtttt aaatgataca tttttttcaa
     1021 attttataca gcaaaagtaa gaaccacata tttcaatttc gttcaaaaaa catgatgatt
     1081 tcgtgtaatt gctgttaaaa atctgtttca agctgatggt ttttaaaaaa gaaaaaattt
     1141 tagaatttca gattgatacc caaaaggaaa atagttccca aattgtaaac ttgattatta
     1201 aggacagaag attttttctt tccatttttt gtatattcat aagtttgctg ggtgcggaaa
     1261 ttttgagcca gcaaatcttt tacaccaaat ttcagttcca ctttttgtcc aattttttta
     1321 ttgaaagaaa aatccagtac atttcgtggc atttcataaa tatcgggaat tactacttcg
     1381 ccttgattga tttgtgcagc gacaagaatt cgtttcccta tcaaattgta aactaatgaa
     1441 gaattgatac cagctttatc attttgataa aacaaagcag catttaccac ataaggtgat
     1501 tgaccttgca atggtcgatt ccgttcattt tgcttatcag aaaaatgtac ttgactgaaa
     1561 atataagatg cattcaaatt aagactaaaa tttttcagcc atttttcaag cgattttttc
     1621 aattccagtt caataccgta atcaacagca ctttgggcat ttttgaatga aaattcattc
     1681 cctactccaa tgtttaccat ttcaatggga tgtatgaaat ttttataaaa taaagccagt
     1741 gtgaacgttt cattcggcga aggataattt tcaaatcgta aatctacatt atgaatggtc
     1801 gcatctttta aatctggatt ccctctcact gaagtttttt cggtaaaatc ataataacaa
     1861 aaaggagaaa tttcgcgaaa ttctggtcgg ttaatggtat aagcataagc caaacgcaat
     1921 aaacttttgt tcgtaaaatt aaaagaagta ttcaaagaag ggaaaaaccg aaaagctgga
     1981 ttttttaccg ttacgggcga actggcatcg taataaccat tcaacaaaag tcgattgtat
     2041 tcagcacgaa caccgccact taaaataaat ttttctacag gaatcgttat tgaaaaatat
     2101 cctgctgcta gcaaattttt tgccttataa gtatttgcta cgttggtctg ttcatccacg
     2161 ctgataactt tgccatgacc cagataaggt gctgtaaaca aatcttcaaa aggaatcgag
     2221 ttgatttgtt catccgaaaa tggaccatat tcttttcgat aagcgatgct acgctctgaa
     2281 aaatttcgag ttttatattt tgtcaaagct ccaactttat aggttggcaa aattttgccg
     2341 attttaatag tattttcaaa atttatgttt gctccactca gatattcgtg cgtggtggta
     2401 aaaagtctcc ctaattcatt gatactgggc acatcaggca aaacataaat atatgttcct
     2461 tcaaccgcct ctttcatact ccaattttgc cgatcaggtt ccaatcgatt ggcataggaa
     2521 aaatcaacgt tccagtccaa tttcttttga tctgaatttt gaagttgatg attgccactt
     2581 aactgtcctg aataaatggt tctacttgaa tattgattgc tgaaatattt aaaatttccc
     2641 tgtcggtaat tattccagcc aaaagtgttg gatgttttat cttcgccgat ttgattcagt
     2701 aaatttttaa attcaattcg agtaccagta gtagtctgat aaccccaatt gtgcatgagt
     2761 ccccatctaa aattggtagc ataaatatct gctgtgtaac gatataaata aacaggtacc
     2821 ccatttactg catcaaattg ttcatacata ttattggtca tattatcacg aacctgattg
     2881 gaagcattgt acgaaaggga agtaatgttt cctatttttg taccatgatt caagttccat
     2941 tttttaccaa tagaaaactg aaatttttgt gcagggaaag ctttataaga ttgtgcaacc
     3001 caattacgat tcaattgtag agcataggca tcctgcaccg taacaggtaa taaattaaga
     3061 tcggaaggaa aatttactgg aagctctctt gctttgctcc ctaatccaat gatatccaat
     3121 ggttttgaag gcaaatgata gatattttta aaagttgatt gattgttgta accaacacta
     3181 tattcaatgt ttagaaaatt tccttccgga atatttttgg taaatatctt aatgaaagct
     3241 ccagtagctt ccgcaggtaa ttctggagaa gcagacttat aagtaatgat gtggtcaatc
     3301 atgttgctgg gaacattatc gaaagaaaaa gcttttacat cgatttcact gctgggcgtt
     3361 ggagcattgt ttaaccagac attattatat cgttgattca agccacgtac gattacaaaa
     3421 cgattttcct gaactgtgat gcccggtacc cgtttgataa cttcagaagc atccgtatca
     3481 agcgttttgc taatttgttg ggaggaaatt ccattaacaa tttgaagaga cgatttcact
     3541 tcccgtaaca gcgaaagttc tgtatctgta cgaggttgag caaccacttg aacacttt
//
LOCUS       DAOTKS010000138         3590 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_21669_length_3590_cov_4.603112, whole genome shotgun sequence.
ACCESSION   DAOTKS010000138 DAOTKS010000000
VERSION     DAOTKS010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21669_length_3590_cov_4.603112"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="P5236_03710"
     CDS             complement(<1..288)
                     /locus_tag="P5236_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63500.1"
                     /translation="MKILEIILPALLVLLTAYLLLDKLLRNEEKKRNFEIFKNNVSII
                     TPIRLRAYERLIILLERTAPNKLILNVLKPNMTSFDLQTELLNTIRQEFAHN"
     gene            complement(348..1298)
                     /locus_tag="P5236_03715"
     CDS             complement(348..1298)
                     /locus_tag="P5236_03715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRU63501.1"
                     /translation="MDISVVIPLYNEEESLTELFEWIERVMNENNFSYEVIFVDDGST
                     DNSWNMIESLKRKTPYIRGIKFRKNYGKSVALHFGFQAAKGDVVITMDADLQDSPEEI
                     PALYAMIKNENYDLVSGWKKKRYDAKLTKNLPSKLYNATARKVTGIKIHDMNCGLKAY
                     RNEVVKNIELYGEMHRYIPYLAKNSGFTKIGEKVVEHNKRQYGKSKFGWNRFVNGYLD
                     LMTLWFLSKFGKKPMHFFGLIGSLMFLLGLIAVIVVGVKKLMALSAHVRAPLVTDSPY
                     FYLALLAMILGTLLFLAGFVAELIIRNSSERNNYLIEKEI"
     gene            complement(1349..1858)
                     /locus_tag="P5236_03720"
     CDS             complement(1349..1858)
                     /locus_tag="P5236_03720"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HRU63502.1"
                     /translation="MTSNIFKSSMIYGLFMGILFAANFFISLPQGIIFRLLSFLIIIL
                     IIYAMYRFSVRYRDNECGGYISYGKAFSFILLTFFFASIISSIFKFFYFQFINPDYLT
                     NLLQESLKIIDSMQLPVNEEFYDQMEKTMKPITYSLQSIWVDVFLGIILGLIMSAFIK
                     KDKNIFEQE"
     gene            complement(1977..3554)
                     /locus_tag="P5236_03725"
     CDS             complement(1977..3554)
                     /locus_tag="P5236_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HRU63503.1"
                     /translation="MNLKDEINRRRTFAIISHPDAGKTTLTEKLLLFGGAIHVAGAVK
                     SNKIKRTATSDWMEIEKQRGISVATSVMGFEYRNYKINILDTPGHQDFAEDTYRTLTA
                     VDSVIIVIDSAKGVEEQTRKLINVCRMRNTPIIVFINKMDREGRDAFDLIDELEKELQ
                     MEVCPLTWPIGQGISFKGVYNRYYNNLQLFTPNKQVVGSSIDISDVCSTELEKCVGEK
                     EAQQLRDELKLISGIYSKFDIEKYLGGELAPIFFGSALNNFGVKELLDCFVEIAPSPR
                     PVEAMERKVNPDEEVFTGFVFKIHANMDPHHRSCIAFVKVCSGKFERNVNYKHVRKNK
                     IIRFSNPTAFMAQKKQIVDEAYAGDIVGIPDNGNFKIGDTLTSGEELHFKGLPSFSPE
                     MFKYLENSDPMKAKQLEKGIQQLMDEGVAQLFINQHNSRKIVGTVGELQFEVIQYRLL
                     HEYNAQCRWETLPLYKACWIESENKEELENFKKRKDQFLAKDKEGRDVFMADSPYILQ
                     MAQNDFKNIKFHFTSEF"
BASE COUNT         1216 a          630 c          519 g         1225 t
ORIGIN      
        1 attgtgggca aattcctgcc tgatagtatt taataattct gtttgaagat cgaaactggt
       61 catatttggt tttaaaacat tcaaaatcaa cttatttggt gctgttcttt ccaataaaat
      121 tatgagccgt tcgtaagctc ttaaacgaat gggggtaata attgaaacat tatttttaaa
      181 aatttcgaag ttacgttttt tttcttcgtt gcggagcaat ttatccaata ataaataagc
      241 agttaacaaa acaagtaatg cgggtaatat aatttcaagt attttcattg aaacaacaat
      301 tttaataaat taactttttt acaaaaagat ataacaagta aagttattta tatttctttt
      361 tctatcaaat aattatttcg ttctgaagaa tttctgataa tcagttccgc tacaaaaccg
      421 gctaaaaaaa gtaaagttcc caaaatcata gccagaagag cgagataaaa ataaggactg
      481 tccgttacca atggggctct gacatgtgca gatagtgcca tgagtttttt aactcctacc
      541 acaatgacag caatcaaacc cagcaaaaac atcaagctcc cgattaaacc aaaaaaatgc
      601 ataggctttt tcccgaattt tgaaagaaac cacaatgtca tcaagtctaa ataaccattt
      661 acaaagcgat tccagccaaa ttttgactta ccgtattgtc gtttgttatg ttcaaccact
      721 ttttcgccta ttttggtaaa accagaattt ttagccagat aaggaatata gcgatgcatc
      781 tcgccataga gttctatatt tttcaccact tcgttacgat aagcttttaa tccgcaattc
      841 atatcatgaa ttttaatacc ggtaactttt cgagcagtag cattgtaaag ttttgaagga
      901 agatttttgg taagttttgc atcatatctt ttctttttcc atcctgatac gagatcatag
      961 ttttcatttt ttatcatggc atacaaagca ggaatttctt ctgggctatc ctgtaaatca
     1021 gcatccattg taattacaac atcgcctttg gctgcttgaa atccaaaatg caaagctacc
     1081 gatttgccat agtttttcct aaattttatg cctctgatat aaggagtttt tcgttttaat
     1141 gattcaatca tattccatga attatctgtg ctgccatcgt ccacaaaaat cacttcataa
     1201 ctgaaattat tttcattcat tactcgttca atccattcga aaagttcggt gagtgattct
     1261 tcttcattat ataaaggaat gacgactgaa atgtccataa ttattacttt ataagccttt
     1321 gttaattata ttattgttgt aaagaatttt attcttgctc aaaaatattc ttgtcttttt
     1381 tgataaaagc tgacattatt aatcccaaaa taattcctaa aaaaacatct acccaaatac
     1441 tttgtaaact ataggtaata ggtttcatcg ttttttccat ttggtcataa aattcctcat
     1501 tgacaggtaa ttgcatacta tcaattattt ttaaactttc ttgcaaaaga ttagtaagat
     1561 agtcaggatt aataaattga aaatagaaaa atttaaaaat agaagatatg atggaagcaa
     1621 aaaagaaagt aagcaagata aatgaaaatg ctttaccata tgagatgtac ccgccacatt
     1681 cattgtcgcg atatctaaca gagaaacggt acatggcata aataatcaaa ataataatca
     1741 aaaaggagag taatctgaat attattcctt ggggaagtga aatgaaaaag ttggcagcaa
     1801 acaaaatccc cataaacaac ccataaatca tggaagattt aaaaatattg gatgtcataa
     1861 caattttttt aaaaattata tttgacaaaa atacaaaaac taaattaaaa aataagttaa
     1921 attgattttc tgggaaaaaa taaggaatta attaaaaaaa caagtaaaaa aagcttttaa
     1981 aactccgaag tgaaatggaa ttttatattt ttaaagtcat tttgagccat ttgtaaaata
     2041 taaggtgaat cagccataaa aacatccctg ccttccttat cttttgccaa aaattgatct
     2101 tttcttttct tgaaattttc cagttcttct ttgttttcac tttcaatcca gcaagcttta
     2161 taaaggggaa gggtttccca tcggcattga gcattgtatt catgcaacaa tcggtactga
     2221 attacttcaa attgaagttc acctactgtc cctacaattt ttcgactatt atgctggtta
     2281 ataaataatt gtgcaactcc ttcgtccatt aattgttgaa tacctttttc taattgttta
     2341 gctttcattg ggtcagaatt ttcaaggtac ttgaacattt caggtgaaaa acttggcaat
     2401 cctttaaaat gcaactcttc acctgaagtt aaagtatcac caattttaaa atttccatta
     2461 tcaggaatac ctacaatgtc gcccgcataa gcttcatcta cgatttgttt cttttgagcc
     2521 ataaacgcag taggattcga aaaacgtata attttatttt ttctgacatg tttataattg
     2581 acatttcgct caaattttcc agaacaaact tttacaaaag caatacaact gcgatggtga
     2641 ggatccatat tagcatgaat tttaaaaaca aaaccagtaa atacttcttc atctggattt
     2701 accttacgct ccatagcttc tacagggcga ggtgaagggg cgatttctac aaaacagtcc
     2761 aataattctt ttacaccaaa attatttaaa gcactaccga aaaaaatagg agctaattca
     2821 cctcccagat atttttcaat atcaaatttt gaataaatac cacttatgag ttttagttcg
     2881 tcgcgaagtt gctgagcttc tttttctcca acacattttt ctaattcggt actacaaaca
     2941 tctgaaatgt caatggaaga accaacgact tgtttatttg gcgtaaaaag ttgtaaattg
     3001 ttataataac gattatatac tcccttaaaa gaaattccct gaccgatagg ccaggtaagc
     3061 ggacaaactt ccatttgcag ttctttttca agttcatcga tcaaatcgaa agcatctctt
     3121 ccttcccggt ccattttgtt aatgaacacg ataatcggag tatttcgcat cctgcaaacg
     3181 ttgattaatt ttcgtgtttg ttcttctaca ccttttgctg aatcaattac aataattaca
     3241 ctatccaccg ctgtaagtgt gcgataagta tcttctgcaa aatcttgatg accaggcgta
     3301 tccagaatat taattttata attccgatat tcaaaaccca taacagaagt tgctaccgaa
     3361 atacctcttt gtttctcaat ttccatccag tctgaagttg cagttctttt gattttattt
     3421 gatttcactg cacctgccac atgaatagca ccgccaaaaa gtaaaagttt ttctgtcaaa
     3481 gtagtttttc cagcatcggg atggctgata atggcaaatg ttcgtcgccg atttatttcg
     3541 tctttgagat tcattttcag atagttaatt ttaatgtaaa cctttgtaaa
//
LOCUS       DAOTKS010000139         3588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_21685_length_3588_cov_5.274837, whole genome shotgun sequence.
ACCESSION   DAOTKS010000139 DAOTKS010000000
VERSION     DAOTKS010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21685_length_3588_cov_5.274837"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3588
                     /gene="rpoC"
                     /locus_tag="P5236_03730"
     CDS             <1..>3588
                     /gene="rpoC"
                     /locus_tag="P5236_03730"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRU63504.1"
                     /translation="LDAIIYYEKYVIIQGGILENGENITQGELLSEEEYLDILDTLPR
                     DNQLLDDSDPNKFIAKMGAEALYDLLSRLDLDALSYELRNKANTDTSQQRKTEALKRL
                     QVVEAFRASRNRNKPEWMILKVIPVIPPELRPLVPLDGGRFATSDLNDLYRRVIIRNN
                     RLKRLIEIKAPEVILRNEKRMLQEAVDSLLDNSRKVSAVKTESNRPLKSLSDSLKGKQ
                     GRFRQNLLGKRVDYSARSVIVVGPELKMHECGLPKNMAAELYKPFIIRKLIERGIVKT
                     VKSAKKIIDRKDPVIWDILEYVMKGHPVLLNRAPTLHRLGIQAFQPKLIEGLAIQLHP
                     LSCTAFNADFDGDQMAVHLPLSNEAILEAQLLMLSSHNILNPANGAPITVPSQDMVLG
                     LYYITKPRKGVKGEGLIFYSPEEAEIAYNEGKVDLHAWIKVRISETNQEGKNVKKIIE
                     TTVGRILFNKCVPPEVGYINELLSKKSLRDIISKVIETCGFARTSQFLDDIKDLGYYM
                     AFKGGLSFNMDDVIIPKEKEQLIQEGNAEIEEIMNNYNMGFITYNERYNQIIDTWTHV
                     NSKLSDVLIKKLSSDQDGFNSVFMMLDSGARGSKEQIRQLSGMRGLMAKPQKSGAEGG
                     QIIENPILSNFKEGLSVLEYFISTHGARKGLADTALKTADAGYLTRRLVDVAHDVTIT
                     QEDCGTLRGLIATELKNNEEVITSLYERILGRVSVHDIYHPQTGELIVAAGQEITEKE
                     AQIIQNSPIEHVEIRSVLTCEAKKGVCAKCYGRNLATGKMIQIGEAVGVIAAQSIGEP
                     GTQLTLRTFHVGGIAGNIAAESKLVLRYDGRLEFDELRTVQPTDNVNYQVVISRLAEM
                     RVIDPKTGIVLSTHNIPYGSKLYVKNGDLGTKGQLICEWDPFNAVIISENEGIIKFED
                     LVENVTFKMETDEATGLREKIIIESKDRNKIPTAYIVDENGNILRTYNLPVGAHIVFE
                     NGDKIKAGQILVKIPRAIGKTGDITGGLPRVTELFEARNPSNPAVVAEIDGEVSLGKI
                     KRGNREIIITSKSGEVKKYLIPLSKQILVQENDFVRAGTPLSDGAITPTDILQIKGPT
                     AVQEYIVNEIQDVYRLQGVKINDKHFEIIVRQMMRKVEIVEAGDTRFLEKQVVDKNEF
                     MEENDRIWGKKVVVDAGDSPNLKPGQIITPRKLRDENSILKRKDLRLVQA"
BASE COUNT         1212 a          545 c          740 g         1091 t
ORIGIN      
        1 gctggatgcc attatttatt atgaaaaata tgtcattatt cagggtggaa ttcttgaaaa
       61 tggagaaaat attacacaag gagaattgct ctcggaggaa gaatatttgg atattttaga
      121 tactcttcca cgcgataatc aacttttaga tgatagtgac cccaacaagt ttattgctaa
      181 gatgggagct gaagcccttt atgatttatt atcacgatta gatttagacg ctttatccta
      241 tgaattaaga aataaagcga atacagatac ttctcaacaa cgtaaaacag aagctttaaa
      301 acggttacaa gtagtagaag ctttcagagc ttcacggaac agaaataaac cagaatggat
      361 gatattgaaa gtgattccag ttattcctcc cgaactacgt cctttagttc ctcttgatgg
      421 tggacggttt gctacttcgg atttgaatga cttatatcga cgtgtcatta ttcggaataa
      481 tcgtttgaaa agactaatcg agattaaagc tccagaagta attttacgta atgaaaaacg
      541 tatgctccag gaagcagttg attcattgct cgataattct cgtaaagtta gtgcagttaa
      601 aacagaatcg aacagacctt taaagtctct ttctgattca ttaaaaggaa aacaaggacg
      661 attccgtcaa aatctgttag gtaagcgtgt ggattattcg gctcgttcgg tgattgtagt
      721 aggtccagaa ttgaaaatgc atgagtgcgg tttgccgaag aatatggctg ctgaacttta
      781 taaacctttt attattcgta aattgattga aagaggaatt gtaaagacag taaaatcggc
      841 aaaaaaaatc attgatagaa aagatcctgt gatttgggat attttggaat atgttatgaa
      901 agggcatcct gtacttttaa accgtgctcc aaccttgcac cgcttaggta ttcaggcttt
      961 tcaaccaaaa ttaattgagg gactggctat tcaacttcat ccgttatcgt gtacagcttt
     1021 caatgctgac tttgatggtg accagatggc agttcatttg cctttaagca acgaagccat
     1081 acttgaagct caattgttga tgttatcttc tcataatatt ttaaatccag ctaacggagc
     1141 tcctattact gttccatcac aagatatggt attgggactt tattacatta ctaaacctcg
     1201 caaaggtgtt aagggagaag gattgatttt ttattcgcca gaagaagctg aaattgctta
     1261 caatgaagga aaagttgact tacatgcatg gatcaaggta agaatatcgg agactaacca
     1321 agaaggtaaa aatgttaaaa aaattataga aactacggtt ggaagaattt tatttaacaa
     1381 atgtgtgcca ccagaagtag gttacatcaa tgaacttctt tcaaaaaaat ctcttcgaga
     1441 tattatttca aaagtaattg aaacatgtgg ttttgctcgt acttctcaat tcctggatga
     1501 tattaaagat ttgggatatt acatggcatt taaaggaggg ctttcgttta atatggatga
     1561 tgtaattatt cctaaagaaa aagagcaact tattcaggaa ggcaatgcag aaattgagga
     1621 aatcatgaat aattacaaca tgggattcat tacttataat gaacgataca atcagattat
     1681 tgatacctgg acccatgtaa attcaaaatt atcagatgtt ttaataaaaa aactttccag
     1741 tgatcaagac ggatttaact ctgtatttat gatgcttgat tctggtgctc gtggctcaaa
     1801 agaacaaatt cgacaattat caggaatgcg aggtttgatg gcaaaacctc aaaaatcagg
     1861 agctgaagga gggcaaatca ttgaaaatcc tatcttgtca aatttcaaag aaggtctttc
     1921 tgtgcttgaa tactttattt ctactcatgg tgcacggaaa ggtttagcag atacagcttt
     1981 aaaaacggct gatgcaggat atttaactcg acgcttagtg gatgtggctc atgatgttac
     2041 tataacgcaa gaagattgcg gaactttacg tggcttgata gctactgaac tcaaaaataa
     2101 tgaagaagtc attacttctc tttatgaacg tattttggga cgtgtttctg tccatgatat
     2161 ttatcatcct caaacaggtg aactaattgt tgctgctggt caagaaatta ctgaaaaaga
     2221 ggcacaaatt attcagaatt ctccgattga gcatgttgaa attcgttccg tattaacttg
     2281 cgaagctaaa aaaggtgttt gtgcaaaatg ttatggacga aatcttgcaa caggcaaaat
     2341 gattcaaatt ggagaagctg ttggagtaat tgctgcacaa tctattggcg aaccaggtac
     2401 acaattaaca cttcgtactt tccatgtggg tggtattgcg ggaaacattg cagccgaatc
     2461 aaaattagta ttaaggtatg atggacgttt agaatttgat gaacttcgca ctgtacaacc
     2521 tactgataat gttaattatc aagtagttat aagtcgtctg gctgaaatgc gggtaattga
     2581 tcctaaaact ggtattgttc tttctactca taatattcct tatggttcaa aattatatgt
     2641 aaagaatgga gatttaggaa caaaaggaca attgatttgt gagtgggatc cgtttaatgc
     2701 agtcattatt tctgaaaatg aaggaattat caaattcgaa gatttagttg agaatgttac
     2761 cttcaaaatg gaaactgatg aagcaacagg attacgtgaa aaaattatca tagagtcaaa
     2821 agacaggaat aaaataccta ctgcttatat tgttgacgaa aatgggaata ttttacgtac
     2881 ctataattta cctgttggtg ctcacattgt gtttgaaaat ggagataaaa taaaagcagg
     2941 acagatttta gtgaaaattc cacgtgccat aggaaaaacg ggtgatatta ctggtggttt
     3001 gccccgagta acggaattat ttgaagctcg gaatccatcc aatcctgctg tggttgctga
     3061 aattgatggc gaagtatcgt taggtaaaat taaacgtggt aatcgtgaaa taattattac
     3121 ctcaaaatca ggcgaagtaa agaagtattt gattcctttg tccaagcaaa ttcttgtcca
     3181 ggaaaatgac tttgtacgag caggtacacc tttgtctgat ggagcaatta ctcctacaga
     3241 tattctgcaa attaaaggtc ctactgctgt tcaggaatac attgtgaatg aaattcaaga
     3301 tgtgtatcga cttcaagggg taaaaataaa tgataagcac tttgaaatta ttgttcgtca
     3361 gatgatgcgt aaagtggaaa ttgttgaagc aggcgatact cgtttccttg aaaaacaagt
     3421 ggttgataag aatgaattta tggaagaaaa tgatcgtatt tggggaaaaa aagtggtggt
     3481 agatgctggt gattcgccca atctgaaacc aggacaaatt ataacccctc gaaaattacg
     3541 tgacgaaaac agtatattaa aacgtaaaga tttgcggctg gttcaagc
//
LOCUS       DAOTKS010000140         3562 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_21868_length_3562_cov_3.749929, whole genome shotgun sequence.
ACCESSION   DAOTKS010000140 DAOTKS010000000
VERSION     DAOTKS010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21868_length_3562_cov_3.749929"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            331..404
                     /locus_tag="P5236_03735"
     tRNA            331..404
                     /locus_tag="P5236_03735"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:365..367,aa:Asp,seq:gtc)
     gene            complement(497..1351)
                     /gene="rfbD"
                     /locus_tag="P5236_03740"
     CDS             complement(497..1351)
                     /gene="rfbD"
                     /locus_tag="P5236_03740"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HRU63505.1"
                     /translation="MKTILVTGSHGQLGSEIQKISDHFSVFQYIYTDIEELDITDKKA
                     LEDFVIKNKAHFLVNCAAYTNVDKAEEEVDLCYKINAEAVKNIGEVAAEQHLKVVHIS
                     TDYVFDGKNFKPYVEEDLPSPVSVYGKSKLAGEEALLKNCQQAMIIRTSWLYSSFGNN
                     FVKKMLSLGKERQELNVVFDQIGTPTYAADLARTILIILQQQSFQPGIYHFSNEGVCS
                     WYDFAKAIFRIKNMECSIQPIESKEFPTKAQRPFYSVLNKAKIKNTFNLCIPHWEESL
                     EKCLSLLP"
     gene            complement(1362..1910)
                     /gene="rfbC"
                     /locus_tag="P5236_03745"
     CDS             complement(1362..1910)
                     /gene="rfbC"
                     /locus_tag="P5236_03745"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HRU63506.1"
                     /translation="MEIIKTPIPDLLVIKPQIFEDSRGYFYEVYNEKRYNEVGITYHF
                     CQDNQSKSTYGVLRGLHYQLSPASQSKLVYVIEGKVWDVAVDLRSTSPTFKQWYGIEL
                     SAENHLQFLIPKGFAHGYAVLSEMAIFAYKCDEFYNPSLERGIIYNDPILAIDWRIPA
                     EKMIISEKDSKHPFFDHAEFNF"
     gene            complement(1976..2848)
                     /gene="rfbA"
                     /locus_tag="P5236_03750"
     CDS             complement(1976..2848)
                     /gene="rfbA"
                     /locus_tag="P5236_03750"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005635387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HRU63507.1"
                     /translation="MKGIVLAGGSGTRLYPITKSISKQIIPVYDKPMIYYPLSILMLA
                     GIREILIISTPQDIHLYERLLNDGSDFGLKIEYAVQPSPDGLAQAFIIGENFIGNENV
                     CMILGDNIFYGYDFSKQLKEAAQLEDGALIFGYYVNDPQRYGVAEFDSEGNVLSLEEK
                     PEHPKSNYAVTGLYFYSNDVVEKAKQLKPSKRGELEITDLNRLYLNENRLKLKIMGRG
                     MAWLDTGTHDSLLEASNFIATIENRQGLKIACLEEIAYRSGYIDREQLVKLAEPLNKN
                     HYGEYLLKIANESK"
     gene            complement(3006..3551)
                     /locus_tag="P5236_03755"
     CDS             complement(3006..3551)
                     /locus_tag="P5236_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007664433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4924 family protein"
                     /protein_id="HRU63508.1"
                     /translation="MFIAQQLKNENICEYLLYMWQIEDVIRAFHLDMDAINSHIIASY
                     PASEIDRKKMYEWYESLIDMMRRENVEEKGHLQLNKNIIIELNEFHQQLLTSGKDHFY
                     NEKFYSIFPYIIELRKKSTTNVSDIELCFNFLYGIMTLRLQKAEIIPATKEAQEKISQ
                     FMSQLAKDYRLYQEGELKFDE"
BASE COUNT         1177 a          631 c          549 g         1205 t
ORIGIN      
        1 aatttatcca caagatgaat tattcataaa caataacaaa tatacagtat tttgtgtaat
       61 tgcagaaata aaaagatgtt gaaacaaagt agcatttacg gaatacccta aatgaataac
      121 ccctaggtaa gcctcgagga attcttttga ttaaaatcta tataagaagg aaaagataaa
      181 attttgactt gaagttgctg ctaaaatatt caaatttaat cactgtgctt tctttcttct
      241 ttcccttttc ttacacttaa aaaagtgaat ggaaagtatt gtagaaatta aaatttgaat
      301 tatctttgca ctaccaaaac aaaatagtct ggtgcggtag ttcagttggt tagaatatcg
      361 gcctgtcacg ccgggggtcg cgggttcaag tcccgtccgc accgcaaaaa attaaaaagc
      421 tcattacttt tcggtagtga gcttttcttt ttatctaaat tgataccaaa aaatttttgg
      481 agataaaaaa cttgcactat ggtaaaagcg aaaggcattt ttctaaactt tcttcccaat
      541 ggggaatgca gagattgaaa gtattcttga ttttggcttt atttaaaacg ctatagaaag
      601 gtcgttgagc tttagttgga aattccttac tttcaattgg ttgaattgaa cattccatat
      661 tttttattcg aaaaatagct tttgcgaaat cataccacga gcaaacccct tcgttggaga
      721 aatgataaat tccaggctga aaagactgct gttgtaaaat gattaaaata gttcttgcta
      781 aatctgctgc ataggtaggc gtcccaatct ggtcaaaaac cacgtttaat tcctggcgtt
      841 cttttcccag acttaacatt tttttgacga aattatttcc aaaagaagaa tacaaccacg
      901 aagtacgaat aatcatagct tgctggcaat ttttcaacaa agcttcttca ccagccaact
      961 tcgattttcc ataaacggaa acaggcgaag gaagatcttc ttccacgtaa ggcttaaaat
     1021 tttttccatc aaaaacataa tcggtagaaa tgtgcactac ttttaaatgt tgttctgcag
     1081 caacttctcc aatatttttt actgcttcag cattaatttt ataacataaa tcaacttcct
     1141 cttctgcttt atctacgttg gtataagcag cacaattaac caaaaaatgg gctttatttt
     1201 ttataacgaa gtcttccagt gcctttttat cggtaatatc cagttcctct atatcggtat
     1261 aaatatattg aaaaacagaa aagtgatcag aaattttttg tatttcgcta cccaattgtc
     1321 catgactgcc cgtaactaaa atagttttca ttttttcatc tttaaaaatt gaattcagca
     1381 tgatcaaaaa aaggatgttt actatctttt tctgaaataa tcattttttc tgctggaatt
     1441 ctccaatcaa tagccagtat agggtcgttg taaataattc cccgttccaa agatggatta
     1501 taaaattcat cacatttata agcaaaaata gccatttcag aaaggacagc ataaccatgt
     1561 gcaaatccct ttggaattag aaactgcaaa tgattttcgg cggaaagttc tatcccgtac
     1621 cattgtttaa aagttggcga agtagaacgt aaatctactg caacatccca cacttttcct
     1681 tcgatgacat aaaccaactt gctttgactt gctggtgaaa gctgataatg taacccgcga
     1741 agaacaccgt aagtagattt ggattgatta tcctgacaaa aatgataagt aattccaact
     1801 tcattatatc ttttctcgtt gtaaacttcg taaaaatagc ctcgtgaatc ctcaaaaatt
     1861 tgtggtttga ttaccagtaa atctggaatt ggtgttttga taatttccat cagaataatt
     1921 attttatttt agattaaaaa ttgcaaatat tttatttaca gaatattata taaatttatt
     1981 ttgattcatt ggcaatttta agaagatatt caccataatg atttttgttt aatggttcgg
     2041 caagcttcac caactgttct cgatcaatat agccactgcg ataagcaatt tcttccagac
     2101 aggctatttt caaaccctga cggttttcaa tcgtagcaat aaaattggaa gcttccagta
     2161 aactatcatg cgtacccgtg tcgagccacg ccattccgcg tcccataatt tttaatttca
     2221 atctattttc attcaaatac aaacggttta agtcagtgat ttccagctcg ccccgtttgg
     2281 aaggttttaa ctgttttgcc ttttcaacaa catcattgct ataaaaatat aagcctgtga
     2341 cagcataatt cgattttgga tgctcaggtt tttcctccag gctcaataca tttccttcac
     2401 tatcaaattc agccacgcca tatcgttgag gatcattaac ataataaccg aaaattaacg
     2461 ctccatcttc taattgtgct gcttctttta gttgttttga aaaatcatac ccataaaaaa
     2521 tgttgtcacc taaaatcata cagacatttt cgttgccaat aaaattttct ccaataataa
     2581 atgcttgtgc caagccatcg ggtgaaggtt gcactgcata ttcaattttt aatccaaaat
     2641 cacttccgtc atttaacaaa cgttcgtaaa gatgaatatc ctggggtgtt gaaatgataa
     2701 gaatttcacg aatacctgcc agcataagga tagacaaggg ataataaatc atgggtttat
     2761 cgtaaacagg aatgatttgt tttgaaatgc ttttggtaat aggataaagt cgtgtaccag
     2821 aaccacctgc cagaacaatg cctttcatag tatataaatt attaaaaatg aattcattaa
     2881 agttataaaa ttattttgtc aactttcaaa ataaataaat ggttttttat ttaaaaaatg
     2941 agtgaaaaat gaatcattca aaattataaa taaattatga aaaaagatat tgaaaaagtt
     3001 gcaatttact catcaaattt cagttcccct tcttgataaa gccgataatc ctttgcgagc
     3061 tggctcatga actgactgat tttttcctgt gcttcttttg tggctggtat gatttctgct
     3121 ttttgtaacc gcaaggtcat aattccatac agaaagttaa aacacaattc gatgtcactg
     3181 acattggtag tagatttttt tcgcagctcg atgatatagg gaaaaatgga ataaaatttt
     3241 tcattgtaaa aatgatcttt cccagaagta agcaactgtt gatgaaattc attcagctcg
     3301 ataataatgt ttttgttcaa ttgcaaatgc cctttttctt cgacattttc tcgcctcatc
     3361 atgtcaatca gactttcgta ccattcatac attttttttc gatcaatttc tgatgctgga
     3421 taagaagcaa taatatggct gttgatggca tccatatcca gatgaaaagc acgaattaca
     3481 tcttcaattt gccacatata aagcaagtat tcgcagatat tttcattttt caattgttga
     3541 gcaataaaca taatttattt at
//
LOCUS       DAOTKS010000141         3549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_21968_length_3549_cov_3.063824, whole genome shotgun sequence.
ACCESSION   DAOTKS010000141 DAOTKS010000000
VERSION     DAOTKS010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21968_length_3549_cov_3.063824"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2939
                     /locus_tag="P5236_03760"
     CDS             <1..2939
                     /locus_tag="P5236_03760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HRU63509.1"
                     /translation="GATLVFSYVGMDKVERKVTGPGKIDVVMSPTAIAIEEVVVTAMG
                     VKSEKKKLNFAVQSVNAEDITDNRSPNFVTSLQGKVSGVSVTTAGGSPNAGSQILLRG
                     ISSVNPAQNNEPLFILDGMVLAGGASAAADINPNDIENITVLKGAAAAALYGQNAANG
                     AIMITTKTAQAGKMSVTANVSLQLDEPANLLELQSMYGPGTLGFYKPEPSGGYGGWGP
                     PLAENEPRYNNVKNYFKTGFYQKYDLSLSGGSEKFQAYASANYSRNDGIVPKDYLDKI
                     GVMLKGTYEINKKLSATLMANMVNSSYRGAGSISSIYSWPINDDIRIYKTGEGKPRYR
                     YISENKKENSPISPLWSRYMDYGLNSSTRNLLQGSLEYKPFKNFNIIGRVSLDQNNYS
                     YDGYAVPRFDDSVILPNPPTSEDYPDPEVLAQKTAEYNAYYNSVPYFNEKDISSVNKD
                     LFGNYSYSQSKRQLITTNVVATYNIPLPKDFTVEILAGGEMKINKSTASSIKGRDFIV
                     PGTYSISNVSEIVGVNDVSLNHRENRNAGIFGELRGDYKGLLNLSVTSRWDWSSTLTL
                     KYNPYYYPSVTGGIVFSELFHLANDWFSYGKLRGNWAKVGKDVASPYLFDRKYTQYPT
                     LPDGGYGADPTLSVASVLKPEMNNSWEIGLDTRFFNNKTRLDIAYYSTTVDNQIVTVR
                     VTPTWGYILQTRNEGSIRNEGFEFTLDQDLLKNKNLSWTAGLNFGLNRGKVLSLPDDV
                     VEIQGTQYGDIFPTAYLNGSTTAISGKDYLRTPDGKIICEANGYPKINPTKSVLIGNR
                     EPDFLMGLKSQFKYKDFSVSFLLDGRKGGDVMNVTGRSLWSSGQHKLLEFYRGRQVVW
                     DGVVEQPDGTYVKNTTPIVLDNRTIINYYYNVSSNFIEDGSYIRLSYVTLGYDFSKLI
                     KKNPYISGLRCSLTGTNLFLLTKYSGSDPQINAETSEGGTGSMGIDSYPVPNTRGFNF
                     SITANF"
     gene            2952..>3549
                     /locus_tag="P5236_03765"
     CDS             2952..>3549
                     /locus_tag="P5236_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007483627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HRU63510.1"
                     /translation="MKTANKFKYLGIVLTVIFGLFLTGCEDWFDINTSKDKPSKVPPD
                     QSLPLLVFYASQICYDHAEYGVYLSQALTTGGRAQVSSYGYKSGWEFLSMNRHPQWRR
                     HYYDIGVNGAELIKEAEKINSKNFILIARTIMLHSTLLTTDAFGDMPVKESYLLSYLP
                     NSPFYSLTKSPAFDPQTDVYEWMLQEADELISLYNDPNW"
BASE COUNT         1165 a          592 c          679 g         1113 t
ORIGIN      
        1 taggagctac tttggtattt agttatgttg ggatggataa agtagaacgc aaagtaacag
       61 gacctggaaa aatagacgta gtaatgtcac caactgctat tgccattgaa gaagtggttg
      121 ttactgctat gggcgtaaaa tcagaaaaga aaaaattaaa ttttgctgta caaagtgtaa
      181 atgcagaaga tattactgac aaccgttctc ctaattttgt tacttcctta caaggaaaag
      241 tttcaggagt tagtgttact actgctggcg gttcgcccaa tgctggttca caaattttgt
      301 tacgtggtat ttcttctgtt aatccagctc aaaacaacga acctttgttt attttggatg
      361 gaatggtatt ggctggagga gcaagtgcag cagcagatat taacccaaac gacattgaaa
      421 atataactgt tttgaaaggt gcggctgccg cagctttgta tggccaaaat gctgccaatg
      481 gcgccattat gattaccaca aaaacggctc aagcgggcaa aatgtctgta acagcaaatg
      541 tgagcttaca attagacgaa ccagctaatt tacttgaatt gcaatctatg tacggacccg
      601 gtacattagg attttataag cctgaaccaa gcggtggtta tggcggatgg ggacctcctt
      661 tagcagaaaa tgagccaaga tacaataatg taaaaaatta ttttaaaaca ggtttttatc
      721 aaaaatatga tctcagcctg agtggaggaa gtgaaaaatt tcaagcttat gcttcagcta
      781 attattccag aaatgatggt atagttccta aagattatct ggataaaatt ggagttatgc
      841 taaaaggaac atacgaaatt aataaaaaat tgtccgctac attgatggca aatatggtta
      901 acagtagcta tcgaggagca ggaagtattt cttctatcta ttcttggcct atcaacgatg
      961 atattagaat ttataaaact ggtgaaggaa agcctcgtta tcgctatatc agtgaaaaca
     1021 aaaaagaaaa ttcgcctatt agcccattat ggagccgata tatggattat ggtttaaatt
     1081 catctacacg aaatttgcta caaggctcac tggaatacaa accttttaaa aattttaata
     1141 tcataggacg tgtaagttta gaccaaaaca attactcgta tgatggttac gctgtacctc
     1201 gatttgatga ctcagtaatt cttcctaatc caccaacatc agaggattat cctgaccctg
     1261 aggttttagc tcaaaaaact gctgagtata atgcttatta taattctgta ccttacttta
     1321 atgaaaaaga tatctcaagc gttaataaag atctatttgg aaattatagt tattctcaaa
     1381 gtaaacggca acttattaca accaacgtag ttgctaccta taacattcca cttcccaaag
     1441 attttactgt tgaaatatta gcgggaggcg aaatgaaaat aaataaaagt actgcttctt
     1501 ctatcaaagg acgtgacttt attgtaccag gtacatatag catatctaat gtaagtgaaa
     1561 tagtaggagt aaatgatgtt agtttaaatc accgtgaaaa ccgaaatgca ggcatctttg
     1621 gtgaattaag aggcgattat aaaggattac ttaacttaag tgtcacctca cgttgggact
     1681 ggtcttcaac gcttacttta aaatataatc cttattacta tccttctgtt actggtggaa
     1741 ttgttttctc tgaattgttc catttagcca atgactggtt ttcttacggt aaattacgtg
     1801 ggaactgggc aaaagtagga aaagatgtag cttcacctta tctattcgac cgaaaatata
     1861 cccaatatcc tactttacct gatggaggct atggagctga tccaacttta tctgttgcaa
     1921 gtgttttaaa acctgaaatg aataattcgt gggaaattgg acttgatact cgtttcttta
     1981 acaacaaaac tcgcttagat attgcctatt attcaactac tgttgataac caaattgtta
     2041 ctgtaagggt tactcctacc tggggttaca ttctccagac cagaaacgaa ggtagtatcc
     2101 gaaatgaagg ttttgaattt actttagatc aagatttatt gaaaaataaa aatctttcat
     2161 ggactgctgg tttaaacttt ggtttaaata gagggaaagt gcttagctta ccagatgatg
     2221 tagtggaaat tcaaggtaca cagtatggag atattttccc cactgcttat ttgaatgggt
     2281 ctactacagc tatttcagga aaagattatt taagaacacc tgatgggaaa ataatttgcg
     2341 aagcaaatgg ctatcctaaa attaatccaa caaaaagtgt attgattggg aacagagaac
     2401 ccgacttttt aatgggttta aaaagccaat tcaagtacaa agatttttct gtaagtttct
     2461 tacttgatgg tagaaaagga ggcgatgtaa tgaatgtaac aggtcgtagt ctctggtcga
     2521 gtggacaaca caaactctta gagttttatc gtggacggca ggtggtatgg gatggagttg
     2581 tagaacaacc tgatggcact tatgtgaaaa acaccactcc tattgtgtta gataaccgaa
     2641 ccattataaa ttattattat aatgtaagtt ccaattttat tgaagatggc tcttatatcc
     2701 gattaagcta tgttacctta ggttatgatt tttctaaact cattaaaaag aatccctaca
     2761 tcagtggttt aagatgttca ttaacaggaa ctaacttgtt cttgttaaca aaatattcag
     2821 ggtccgatcc tcaaataaat gctgaaacca gcgaaggagg tacagggtct atgggaattg
     2881 atagctatcc cgtacccaat accagaggtt ttaatttttc tattactgct aacttttaaa
     2941 tcgtaacaac aatgaaaaca gctaataaat ttaaatattt aggtatcgtt ttaactgtca
     3001 tttttggcct ttttttaact ggctgtgaag attggttcga tatcaatacc agcaaagata
     3061 aaccttcgaa agttccccct gatcagtctt tgcccttgct cgttttttat gcttcacaaa
     3121 tttgctatga ccatgctgag tatggtgtct atttgtcaca agcattgacc acgggcggac
     3181 gtgcacaagt aagttcgtat ggctataaaa gtggttggga atttttatcc atgaatcgac
     3241 atccacaatg gagacgacac tactatgata ttggagtgaa tggtgctgaa ttaattaaag
     3301 aagctgaaaa aattaattca aaaaacttta ttctcattgc acgcaccatt atgctacact
     3361 ctactttatt gactactgat gcttttggtg atatgcctgt aaaagaatca tacttattgt
     3421 cttatttacc caattcacct ttttatagct taaccaaatc accagcattt gatcctcaaa
     3481 ctgatgttta cgaatggatg ttgcaagaag ctgatgaatt aataagttta tataatgatc
     3541 ctaactggg
//
LOCUS       DAOTKS010000142         3524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_22178_length_3524_cov_4.067166, whole genome shotgun sequence.
ACCESSION   DAOTKS010000142 DAOTKS010000000
VERSION     DAOTKS010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22178_length_3524_cov_4.067166"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /gene="dapA"
                     /locus_tag="P5236_03770"
     CDS             complement(<1..624)
                     /gene="dapA"
                     /locus_tag="P5236_03770"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008148263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRU63511.1"
                     /translation="MNMYNKLTGMGVALVTPFKNDDSIDFEAFGRLIEYIIKNGADYL
                     VVCGTTAETPTLTEDEKDELARFAVQQNAGRLPIVLGIGGNNTKAVVEKIKTTDLSRI
                     DAILSVTPYYNKPSQEGLYQHFASIAKVSPLPIILYNVPGRTGVNMTAETTLRLAKDF
                     SNICAIKEASGNFVQIGEIIKNKPEDFLVISGDDGVAYPLITMGGVGV"
     gene            complement(661..1224)
                     /locus_tag="P5236_03775"
     CDS             complement(661..1224)
                     /locus_tag="P5236_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63512.1"
                     /translation="MIIDKIAKYQLKKEVKRYNLSTAREKRFINYDQARNFLLIFESD
                     ENEKNLPIKKIIEKLKSDDKKVTAIGYLNKKKLCTPFLLDYKIFNSRQTNFFKKPNEF
                     ILKELRNYQFDIVIDLSFHNVIPLQYVVLYVNAFLKVGLKNADLSIYDFIFNITNQFK
                     EELSFFDNINNIFNQIIFYLKNIQSSD"
     gene            complement(1255..3258)
                     /gene="ligA"
                     /locus_tag="P5236_03780"
     CDS             complement(1255..3258)
                     /gene="ligA"
                     /locus_tag="P5236_03780"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRU63513.1"
                     /translation="MSIKEQIEALRKELEQHNYYYYVLSQPIISDFEYDMKLKQLQEL
                     EEKYPEYYDPYSPTQRVGSDLTEGFEQAYHAYPMLSLSNTYTEEEVKEFYERVSRTLN
                     EPFELVGELKYDGVSISITYENGVMIRAVTRGDGEKGDVVTANVKTIKSIPLRLRGDY
                     PSKFEIRGEVLMSWEVFDKLNKEREAQEEPLFANPRNATAGTIKLLSPAIVASRNLDA
                     YFYYVLGEELPYRTHYDNLQAAKTWGFKISSDMKVCKNLEEVFDFIHYWDVERRKLPI
                     PTDGVVLKVNSLDQQRKLGATAKFPRWAIAFKFQAEKALTKLLSVSYQVGRTGAVTPV
                     ANLEPVVLSGTTVKRASLHNADIIESLDLHIGDMVYVEKGGEIIPKITGVEKSARIQV
                     GEKVQFIKNCPECGTPLIRYPGEAAHYCPNENGCPPQIKGKIEHFVSRKAMNIEGLGT
                     ETINLLYNKGLLRNIADIYDLKFSDLVNLERMGEKSANNLLNSIEKSKQVPFERVLFA
                     LGIRFVGETVAKKLAAHFKSIDALQHATYEELLEVEEIGDKIAQSVIQFFSDPKNLEI
                     IQRLKAAGVQMSVSEENQLLKGNELEGKSIVISGVFKLHSRDEYKKMIEEHGGKNVSS
                     VSSKTSFILAGDNMGPEKLKKAQQLGIPLIDESTFLAMINEKQ"
BASE COUNT         1088 a          652 c          532 g         1252 t
ORIGIN      
        1 gacaccaact cctcccattg taatcagtgg ataagcaaca ccatcatcac cagaaattac
       61 taaaaaatct tcaggtttgt tttttataat ttcaccaatt tgtacaaagt tacctgatgc
      121 ttcttttata gcacaaatat tagaaaaatc tttagccaaa cgtaaagtag tttcggctgt
      181 catatttacg cccgttctgc ctggaacatt atagagaata ataggaagtg gtgaaacttt
      241 tgctatactt gcaaaatgtt ggtaaagtcc ttcctgcgaa ggtttattat aatatggtgt
      301 aacggagagt atcgcatcaa ttcttgataa atcagtagtt ttaatctttt ctactacagc
      361 ttttgtatta tttccaccga tgcccagcac aattggtaat cgtccggcat tttgttgaac
      421 agcaaaacga gccagttcat ccttttcgtc ttcagttaag gtgggtgttt cagcagttgt
      481 tccacacact accagataat cagcaccatt tttaattatg tattctatta accttccaaa
      541 agcctcaaaa tcaattgaat catcattttt aaaaggagtt accaaagcta ctcccattcc
      601 cgttaattta ttatacatgt tcatagaaaa tatattttaa ttcatcacaa aattacgctt
      661 ttaatcagaa gattgaatat tttttaagta aaatattatt tgattaaaaa tattatttat
      721 gttgtcaaag aaggataatt cctccttaaa ttgattcgta atgttgaaaa taaaatcgta
      781 aattgacaaa tcagcgtttt tcaatcctac tttaagaaaa gcattgacat aaagcaccac
      841 atattgcaaa ggaatgacat tatgaaatga taaatcaatt actatatcaa actgatagtt
      901 tctcaattct ttcaaaataa actcgttagg ctttttaaaa aaattggttt gtcgagaatt
      961 aaaaatttta taatcaagca aaaatggtgt acagagtttt ttcttgttca aataaccaat
     1021 agcagttact tttttgtcat cagattttaa tttttctata atttttttga taggaagatt
     1081 tttttcattt tcatcgcttt caaaaatcaa caggaaattc cgtgcttgat catagtttat
     1141 aaaacgtttt tctcgagcgg tagataaatt ataccttttt acttcttttt ttaactgata
     1201 tttagctatt ttatcaatta tcataaaatt aaaaccttag gttaacaaag tttattattg
     1261 cttttcgttg atcatggcta aaaacgtact ttcatcaatc aatgggatgc ccaattgttg
     1321 agcttttttg agtttttcgg gtcccatatt atctcctgcc agaataaaag atgtttttga
     1381 agaaacgctg ctcacatttt ttccaccatg ttcttcaatc atttttttgt attcatctcg
     1441 tgaatgaagt ttgaaaaccc cactaatcac aatagatttc ccttccaact catttccttt
     1501 caataattga ttttcttcag aaacactcat ttgaacacca gcagctttga gtcgttgaat
     1561 aatttccaga tttttaggat cagaaaaaaa ttggatcaca ctttgtgcta ttttgtcgcc
     1621 aatttcttct acttccagga gttcttcgta ggtggcatgt tgcaaagcat cgatagattt
     1681 gaaatgagcg gctaattttt tagctactgt ttcgcctaca aaacgaattc ccagagcaaa
     1741 caaaactctt tcaaaaggaa cttgtttaga tttttctata gaattcaaaa gattgttagc
     1801 tgacttttct cccattcgtt ccaaattcac taaatcagaa aattttaaat cataaatatc
     1861 ggctatattt cgcaaaagac ctttgttata aagcagattt atggtttctg ttcccaatcc
     1921 ttcaatgttc attgcttttc ggctgacgaa atgttcgatt tttcctttga tttgtggcgg
     1981 acaaccattt tcgttcgggc aataatgagc agcttcgcca ggataacgaa tcagaggcgt
     2041 tccacattca ggacaatttt taataaattg aaccttttct ccgacttgta ttcttgcact
     2101 tttttctaca cctgtgattt taggtataat ttctccgcct ttttcaacat ataccatgtc
     2161 gccgatatgt aaatcgagtg attcgataat gtctgcattg tgtaacgaag cacgttttac
     2221 cgttgttccc gaaagtacta cgggttccag atttgctaca ggagttactg cacctgtacg
     2281 tccaacttga tacgaaacgg ataaaagttt tgtcagtgct ttttccgcct gaaacttaaa
     2341 agcaattgcc cagcgaggaa attttgcagt agctcctaac tttcgttgtt gatctaaaga
     2401 atttactttt agcacaacac catcagttgg gataggtaat tttcgtcgtt caacatccca
     2461 ataatgaata aaatcaaata cttcttcaag atttttgcag actttcatgt cacttgaaat
     2521 tttaaatccc catgttttag ccgcttgaag attatcgtaa tgagttcggt atggcaattc
     2581 ttctcccaac acatagtaaa aataagcatc caggtttcga gaagcaacaa ttgctggact
     2641 taaaagtttt atagtgccag cagtagcatt tcgaggattg gcaaaaagtg gttcttcttg
     2701 ggcctccctt tccttattca atttatcaaa aacttcccaa gacatgagaa cttctccacg
     2761 aatttcaaac tttgatggat aatcacctcg caatcgcaat gggatacttt taatagtttt
     2821 cacattggct gttacaacat cacctttttc gccatcacca cgtgtcacag cacgaatcat
     2881 cacaccattt tcataagtta tggaaattga aacaccatca tatttcagct ctcctaccag
     2941 ttcaaaaggt tcgtttaatg ttcttgaaac tcgctcgtaa aattccttta cttcttcttc
     3001 tgtgtaagta ttactcaacg aaagcatggg ataagcgtga taagcctgtt caaaaccttc
     3061 agtcaaatca ctgccaaccc gttgagttgg tgaataagga tcgtaatatt cgggatattt
     3121 ttcttcgagc tcttgtaatt gtttcagttt catatcgtat tcgaaatctg aaataatcgg
     3181 ctgagaaagt acataataat aataattatg ttgctcgagt tccttacgca gtgcttctat
     3241 ctgttcttta atgctcattt ccataatcaa aatgtgtttc taaaaataaa tccaagaatt
     3301 tattgaactg ttttatacca agttgtaatc attaatataa ttttaatagc atcgattgat
     3361 ttcgattaaa tgatttttta cgatagatat ttgtttagca aaatgtttga ttttatcagt
     3421 agttaaaaat caggagattt tattttttgt gcgacgtagc caaaaggcta cgccaaacaa
     3481 agatatgagg ctttgccaca aaccccactt acttttttat tatt
//
LOCUS       DAOTKS010000143         3508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_22311_length_3508_cov_3.377643, whole genome shotgun sequence.
ACCESSION   DAOTKS010000143 DAOTKS010000000
VERSION     DAOTKS010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22311_length_3508_cov_3.377643"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="P5236_03785"
     CDS             <1..247
                     /locus_tag="P5236_03785"
                     /inference="COORDINATES: protein motif:HMM:NF027479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HRU63514.1"
                     /translation="SDNQVPSGRNYVAVFVPCKIKEAKVQSLHALLISDYTVGENFTY
                     YFGGGWNKWGYPTDNDWFNAVYQFTEKISQPLIISLK"
     gene            379..1077
                     /gene="tsaB"
                     /locus_tag="P5236_03790"
     CDS             379..1077
                     /gene="tsaB"
                     /locus_tag="P5236_03790"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRU63515.1"
                     /translation="MENQTVTILEIETSTNVCSVALSEENSCLFSKVNKEGLNHAALL
                     SNYIQEALQVLKERNKKLNAVAVSSGPGSYTGLRIGVATAKGLCYGLNIPLISVNTLE
                     ILTIPVLQIIKEENALFCPMIDARRMEVYTAFYIAHLEMIRPVSSDIINNDSYKEILE
                     KNPIYFFGNGMNKCKEYLQHPNAHFIENILPLATSMIPLSLKAYHEKQFENTAYFEPF
                     YLKEFQTTHSKRKL"
     gene            complement(1170..2618)
                     /locus_tag="P5236_03795"
     CDS             complement(1170..2618)
                     /locus_tag="P5236_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRU63516.1"
                     /translation="MTTMKENNVKMTNYRWVICFMLFFATTINYLDRQVLSLTWKDFI
                     APEFHWTNNDYGTITALFSIFYAISMLFAGRLVDKLDTKKGFMWAIGVWSFGAILHAF
                     CGIATSGILTGHWLVSFGEAKQFISGVDNVAKIVNVSVTLFIFARIVLAIGESGNFPA
                     AIKATAEFFPKKDRAFATSIFNSGAQIGALIAPLTVPFIAKAWGWEMAFIVMGLLGFI
                     WMGFWVFVYKKPEVHPKINELELAYIQQDKDEEIEEVAPIEKKKVTFAQVFKYKQTWA
                     FAFGKFMTDGVWWFYLFWTPAYLSSVYGMDATESAVPIFILYLITMLSLVGGWLPTYF
                     VDKKNMHPYAGRMKAMLIFAFFPLLALLAQPLGTYSYWLPIIIIGIAGAAHQSWSANI
                     FSTVGDMFPKHAVATITGIGGMAGGIGSFLINKGSGALFDYSEAVGMRFMGFEGIDSG
                     YFIIFSICAVAYLIGWMVMKLLVPKYKPIVDL"
     gene            complement(2623..3291)
                     /locus_tag="P5236_03800"
     CDS             complement(2623..3291)
                     /locus_tag="P5236_03800"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HRU63517.1"
                     /translation="MKFSKIQVLTKMAETGIVPVFYNSNIETAKNVVKACYEGGIRVF
                     EFTNRGEFAHEVFNEVVKYAEKECPEMIVGVGSVVDAPTAALYIQLGANFVVGPLFNP
                     DVAKVCNRRLILYVPGCGSVTEIGFAQEAGCDLCKVFPGEVLGPAFVKALKAPMPWSN
                     LMVTGGVKPEENNLKSWFDAGVMCVGMGSNLFPAEMIKANNWQGITNLCITSLSIIQG
                     LKKS"
     gene            complement(3320..>3508)
                     /locus_tag="P5236_03805"
     CDS             complement(3320..>3508)
                     /locus_tag="P5236_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HRU63518.1"
                     /translation="GDSFMGGLIYGLLNYPNDDQHALEFAVAASCLKHTIYGDFNLVS
                     VAEVENLMKGDVSGRVAR"
BASE COUNT         1233 a          685 c          509 g         1081 t
ORIGIN      
        1 ttctgataat caagttccct caggacgaaa ttatgtagct gtttttgtcc cttgcaaaat
       61 caaggaagcc aaagttcaaa gtttacatgc tttacttatc tctgattata cagtgggaga
      121 aaatttcact tattattttg gtggtggttg gaacaaatgg ggatatccaa ctgataatga
      181 ttggtttaat gcagtatatc aatttacaga aaaaattagc cagcctttga taataagttt
      241 aaagtaataa aaaaattcta ttattttatc aaagataaaa aatatcagta tttcattctt
      301 tttaaataaa tctttacatt tgcaataaca tttgggacag tgtctttagg gcactgtctt
      361 ttaatttata taaaataaat ggaaaatcaa acagttacaa tactcgagat tgaaacatcg
      421 acaaatgtat gttctgttgc attaagcgaa gaaaattcct gccttttctc aaaggtaaat
      481 aaagaagggt tgaatcacgc tgctttatta agtaactata ttcaagaagc acttcaagtc
      541 cttaaagaaa gaaataaaaa attaaatgcc gttgcagtga gtagtggacc gggttcttat
      601 actggtctgc gaattggggt cgctaccgct aaaggtttat gttatggttt aaatattccg
      661 ttgatttcag tcaataccct tgaaatactt accattcctg ttttacaaat aataaaagag
      721 gaaaatgctt tattttgtcc gatgattgat gctcgaagga tggaagttta tactgctttc
      781 tatattgctc atttagaaat gataaggccc gtatcttcag atataattaa taatgattca
      841 tataaagaaa tccttgaaaa aaatcctatt tacttttttg ggaatggcat gaataaatgt
      901 aaagaatatc ttcaacatcc gaatgcacat tttatcgaaa acattttacc acttgcgacc
      961 tctatgatac ccctttctct gaaagcttat catgaaaagc aatttgaaaa tactgcatat
     1021 tttgaacctt tttacctcaa agaatttcaa accactcatt caaagagaaa attataaact
     1081 aattctcata atattttttt aatgaaaaac atatgaagtt ttaaaaaaga aagggaagca
     1141 aaattttact cccctttcat tttaataaat tataggtcaa caataggctt atatttgggt
     1201 actaataatt tcattaccat ccagcctatt aaataagcta cagcacaaat cgaaaaaata
     1261 ataaaatatc ctgaatctat tccctcaaaa cccataaatc tcatacctac agcttcagaa
     1321 taatcaaata aagcacctga tcccttattt atcaagaagg aaccaatacc acctgccata
     1381 ccgccaatac cagtaatagt agcgacagca tgttttggga acatatctcc aacagtggaa
     1441 aatatgtttg ctgaccacga ttgatgggca gcacctgcta tacctataat gataataggg
     1501 agccaataag aataagttcc taagggttga gctaaaagtg ctaacaacgg gaaaaaagca
     1561 aaaataagca tagccttcat acgtccagca taaggatgca tattcttctt atccacaaaa
     1621 tatgttggca gccatcctcc aaccaatgaa agcatggtaa ttaaatacaa tataaaaata
     1681 ggaacagcac tttcagtggc atccattcca taaacggaac ttaaataagc aggtgtccag
     1741 aaaagataaa accaccatac accatctgtc ataaacttgc caaaagcaaa agcccaggtt
     1801 tgcttatatt taaagacctg agcaaaagtt acttttttct tttctatagg ggcaacctct
     1861 tctatttcct catccttgtc ctgttgaata tatgccaatt ctaattcatt tattttggga
     1921 tgaacctccg gtttcttata aacaaaaacc caaaatccca tccaaataaa acccagtaac
     1981 cccataacga taaaagccat ttcccaaccc catgcttttg ctataaaagg aacagttaaa
     2041 ggagcaatta aagcaccaat ttgagctcct gaattaaaaa tgctggtagc aaatgctcta
     2101 tcttttttag ggaaaaattc tgctgttgcc ttaattgccg cagggaaatt tcctgattcg
     2161 ccaattgcca gaacgattcg tgcgaaaata aataaggtaa cactaacatt tacaattttc
     2221 gccacattat ctactccgct aataaattgc ttggcttccc caaaacttac aagccaatga
     2281 cccgtcagaa ttcctgaagt agcgattcca caaaaagcat gtaaaattgc tccaaaagac
     2341 cacacgccaa ttgcccacat aaaacctttt ttggtatcta atttatctac caaacgtcca
     2401 gcaaataaca tacttattgc ataaaatatt gaaaaaagag ctgttattgt tccataatca
     2461 ttatttgtcc aatgaaattc gggagcaata aaatctttcc atgttaacga caaaacctga
     2521 cgatccagat agttaatggt ggttgcaaaa aatagcataa aacaaattac ccatcggtaa
     2581 ttggtcattt ttacattatt ttctttcatg gtagtcatat ttttatgatt tttttaatcc
     2641 ttgaatgata gaaagtgaag taatacataa attagtaatt ccttgccagt tgttagcttt
     2701 tatcatttca gcaggaaata gattagaacc cattcctaca cacatcacac cggcatcaaa
     2761 ccaacttttc agattatttt cttctggctt gacaccccct gttaccatta aatttgacca
     2821 tggcatgggg gctttcaatg cttttacaaa agcgggtccg agaacttcac caggaaatac
     2881 tttacacaaa tcacatccgg cttcttgagc aaatcctatt tcggttaccg acccacagcc
     2941 agggacataa agaattaaac ggcgattgca aacttttgca acatctggat tgaacaaagg
     3001 tcctactaca aaattggctc ccaactgaat gtataacgca gcagttggag catcaaccac
     3061 agaaccaaca cccactatca tttctggaca ttctttttca gcatatttca ctacctcatt
     3121 aaaaacttca tgagcaaatt caccgcggtt ggtaaattca aaaactcgaa ttcctccttc
     3181 ataacaagcc tttaccacat tttttgcggt ttcaatattg ctattataaa aaactggaac
     3241 tattccagtt tcagccattt ttgttaaaac ttgaattttt gaaaatttca tttctttatt
     3301 gttattttta aattcataat tatcttgcta ctcttcctga aacatcgcct ttcatcaaat
     3361 tttctacttc tgctactgag accaaattaa aatcaccata aatagtgtgt tttaaacaag
     3421 aagcagccac agcaaattca agagcatgtt ggtcatcatt tggataatta agtaaaccat
     3481 aaataagtcc tcccataaat gaatcacc
//
LOCUS       DAOTKS010000144         3480 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_22531_length_3480_cov_3.883212, whole genome shotgun sequence.
ACCESSION   DAOTKS010000144 DAOTKS010000000
VERSION     DAOTKS010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22531_length_3480_cov_3.883212"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="P5236_03810"
     CDS             complement(<1..349)
                     /locus_tag="P5236_03810"
                     /inference="COORDINATES: protein motif:HMM:NF022187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRU63519.1"
                     /translation="MDIVLIGAGNVANQIAKTFQKKNISIKQIYSRTLESAQKLAKEI
                     NVSFTNNLAEIDRQADVYFYAVKDSALEEIIRKINIREGLHIHTAGSIPISVFEGFAE
                     KYGVFYPLQTFSKT"
     gene            complement(464..991)
                     /locus_tag="P5236_03815"
     CDS             complement(464..991)
                     /locus_tag="P5236_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRU63520.1"
                     /translation="MNSFFELIKTRRSIRKYMDEKIDAEKISLITKAALMSPSSKRSN
                     PWEFIVVQDAQMLEKLANCKPNSSQFLAHAPLGIVVIADTTKSDIWIEDASIASIIIQ
                     LQAHDLGLGSCWIQVYNRKKNEQTPAEDYIKDLLQIPERYSVLCIISIGYPNEVRKPH
                     EEEKLQYEKIHNEKF"
     gene            complement(1011..1832)
                     /locus_tag="P5236_03820"
     CDS             complement(1011..1832)
                     /locus_tag="P5236_03820"
                     /inference="COORDINATES: protein motif:HMM:NF025115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HRU63521.1"
                     /translation="MKRKIILFSFISAVFILFFTGCISKTVKTKILEYSNKFYLTSDT
                     TKGGLSLSIQVEMPVKFHNKNVLDTIRKQLISRIFGEEYQSLPLDSVIPAFANSQRDD
                     YLENNLAMLKLLKKDSPYNFNYEHNIEAFALWDDQNIFSYGIKRYVFMGGAHGLSNHI
                     LLNFDLKTGEQITEKDLFKDNYNEFLTELLINRLIEENKELSSIDDLKKTDYWVDSIR
                     PNSNFYITDEGISYIFNPYEISPYYVGETEIFLPYARLKNILKPGNPIEYLYREK"
     gene            complement(1965..3329)
                     /locus_tag="P5236_03825"
     CDS             complement(1965..3329)
                     /locus_tag="P5236_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HRU63522.1"
                     /translation="MEKTKKIILPEKEIPSQWYNIVAELPNKPMPILHPGTKQPIKEE
                     DLYPIFAKELSHQELNQTDLWIDIPEEVREKYKIYRPTPLIRAYELEKALDTPAHIYY
                     KNESVSPAGSHKLNTALAQAYYCKKEGITNITTETGAGQWGTALALAAKFFGLELTVY
                     MVKISYEQKPYRRSLMQLWGAQVIPSPSNTTKAGQKILAERPNYMGSLGTAISEAIEL
                     ATEKPNCKYTLGSVLNHVSLHQTVIGLEAEKQMEIAGEYPDIVIGCFGGGSNFSGISF
                     PFIRHNIRNGKKTRFIAAEPTSCPKLTRGIFEYDFGDEAGYTPLLPMFTLGHNFAPAH
                     IHAGGLRYHGAGTIVSQLMKDNLMEAVDIDQLDSFKAGLLFAQTEGIVPAPESAHAIA
                     ATINEALKAKEEGTPKTILFNLSGHGFLDMSAYDQYLSGELTNYTLTEEEITRNVNKL
                     EHLV"
BASE COUNT         1150 a          555 c          535 g         1240 t
ORIGIN      
        1 ttgtttttga aaatgtttgc aaaggataaa atacgccata tttttctgca aatccttcaa
       61 aaacactgat aggaatgctt ccagcggtat gaatatgtaa accttctcga atattaattt
      121 ttctgataat ttcttctaaa gcactgtcct tcactgcata aaaatagaca tcagcctgac
      181 ggtcaatttc tgccaaatta tttgtaaacg aaacatttat ttctttagca agcttttgtg
      241 cactttctaa tgttcggcta taaatttgct tgatcgaaat attttttttc tggaaagttt
      301 tagcaatttg atttgctaca tttccggcgc caatcagtac aatgtccatg aattaaatac
      361 ttgaaaaatt aattgaactg taaaaagctt ttgtttcctt cttgaaaatt atttttaaaa
      421 tgtaaaaacg aaaagtaaaa ggatttttct gccgaaagaa taattaaaat ttctcattat
      481 gaattttttc gtactgaagt ttttcttctt catgaggttt gcgaacttca ttgggataac
      541 ctattgaaat aatgcagaga acagaatatc gttcaggaat ctgaagcaag tctttgatgt
      601 aatcttcagc aggagtctgt tcattttttt ttctgttata aacctgaatc caacaacttc
      661 ccagacccag atcgtgtgct tgcagttgga taataataga agcaattgag gcatcttcta
      721 tccaaatatc gctttttgtt gtgtcggcaa taacaactat gcccaatgga gcatgagcga
      781 gaaattggga gctgtttggc ttacaatttg ccaatttttc cagcatttga gcatcttgaa
      841 ccacaataaa ttcccacgga ttacttcttt ttgaagaagg agacatcaac gcagcttttg
      901 taatcaaaga aattttttca gcgtcaattt tttcgtccat gtatttgcga atactacgac
      961 gagtttttat gagttcaaaa aatgagttca tttcctaaat aaataatttg ttatttttca
     1021 cgataaaggt attcaatcgg attacctggt tttaaaatgt ttttcaaacg agcatagggc
     1081 aaaaaaattt ctgtttcacc cacataataa ggagaaattt cataaggatt gaaaatgtaa
     1141 cttattcctt catctgtaat gtaaaaattg ctatttggtc ggatagaatc gacccagtaa
     1201 tcagtttttt tcaagtcatc aatggaacta agttctttgt tttcttctat aagacgattg
     1261 atcaataatt ctgtaagaaa ttcattataa ttatctttaa ataaatcttt ttctgtaatt
     1321 tgttctccag tttttaaatc aaaatttagt aaaatatggt tacttaatcc atgagctcct
     1381 cccataaaaa catatcgctt gatgccataa gaaaagatat tttgatcgtc ccaaagtgca
     1441 aaagcctcaa tattatgctc gtaattaaaa ttatagggac tgtctttttt taatagtttt
     1501 aacatggcta aattgttttc caaatagtca tctcgctggc tatttgcaaa agctggaatt
     1561 actgaatcaa gtggcaatga ctgatattct tctccaaata ttctggaaat gagttgttta
     1621 cgaatagtat ccaatacatt tttattatga aattttactg gcatttctac ctgaatggaa
     1681 agcgataatc ctcctttggt agtatcagaa gttaaataaa atttattgga atattccaat
     1741 atttttgttt tcactgtttt tgaaatgcaa cctgtgaaaa acaaaataaa aaccgcacta
     1801 ataaaagaaa ataaaataat ttttcttttc attttttgta gatttttttc atcgacaaaa
     1861 ttacgataat aaacaaaaac aacttacttt tttgctaact tataatttga aaaaagcaag
     1921 acaaacaaaa taagtttgta cttgctttga aatattacca attattaaac cagatgttct
     1981 aatttgttaa catttctggt aatttcttcc tctgttaaag tataattagt taattcacca
     2041 gataaatatt gatcataggc tgacatatct aaaaatccat gtcctgataa attaaataaa
     2101 atagtttttg gggttccttc ttcttttgct tttaaagctt cattaatagt agcagcaata
     2161 gcatgtgctg attccggtgc aggaacaatc ccttctgtct gagcaaagag caaaccggct
     2221 ttgaatgaat caagttgatc aatatcaact gcttccatta aattatcttt cattaattga
     2281 cttacaattg tgccggcacc atgataacga agtccgcctg catgaatatg tgcaggagca
     2341 aaattatgtc ctaaggtaaa catgggtaaa agaggtgtat aaccagcttc atcaccaaag
     2401 tcatattcaa atattcccct tgttaatttt ggacaagaag taggttcagc agctataaaa
     2461 cgtgtttttt taccatttct aatattgtga cgaataaacg gaaaagaaat tcccgaaaaa
     2521 tttgaaccac cgccaaaaca tccaataact atatcaggat attctcctgc tatttccatt
     2581 tgtttttcag cttctaaccc tattacagtt tgatgaagcg aaacgtgatt aaggacactt
     2641 cccaaagtat atttacaatt tggtttttca gtggcaagtt ctattgcttc agaaatggct
     2701 gttccaagac tgcccatata atttggacgt tcagctaaaa ttttttgtcc agctttagtg
     2761 gtattgcttg gggaagggat tacctgagct ccccacaatt gcatcaatga acgacgataa
     2821 ggtttttgtt cataactaat ttttaccata taaacagtta attccaaacc aaaaaattta
     2881 gcagcaagag ctaaagctgt tccccattgt ccagcaccag tttcagtggt aatgttggta
     2941 atcccttctt ttttacaata ataagcttga gctaaagcag tatttagttt atgtgaacct
     3001 gctgggctga cactttcatt tttgtaataa atatgggcag gagtatctaa agctttttct
     3061 aattcataag ctcttatcaa aggggtaggc cgataaattt tatatttttc tcgtacttct
     3121 tctggaatat caatccataa atcagtttga ttaagttctt gatgagataa ttcctttgca
     3181 aaaataggat ataaatcttc ttctttaata ggttgttttg ttccaggatg aagaataggc
     3241 ataggtttat ttggtagttc tgcaacaata ttataccact gggaaggtat ttctttttca
     3301 ggtaaaataa ttttttttgt cttttccata aataattaat ttattgtaat aatttattgt
     3361 aaattaatat aaatttgagc atccaaagat actacaaaca tttgaatgtt taaaaaaaag
     3421 atgtttttct aacaaaattt atttatagaa gaagggaata aaaaattaag gtaagaattt
//
LOCUS       DAOTKS010000145         3461 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_22667_length_3461_cov_2.896653, whole genome shotgun sequence.
ACCESSION   DAOTKS010000145 DAOTKS010000000
VERSION     DAOTKS010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22667_length_3461_cov_2.896653"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..201
                     /locus_tag="P5236_03830"
     tRNA            129..201
                     /locus_tag="P5236_03830"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:162..164,aa:Thr,seq:cgt)
     gene            230..583
                     /locus_tag="P5236_03835"
     CDS             230..583
                     /locus_tag="P5236_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63523.1"
                     /translation="MLMFDNILFATFIPQILMLVGYLFCLLAPKATTNSTTFSISESS
                     KTISYYSSENQTLNNSTFVVDFHSDVPCNIENNKNLTIPICKCEIRLPIQKVFFKISE
                     KFSFKLFCRPPPVFC"
     gene            718..>1322
                     /locus_tag="P5236_03840"
     CDS             718..>1322
                     /locus_tag="P5236_03840"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRU63524.1"
                     /translation="MKKNLIAVVLLFPLSLYAEEEQKNDSLRTIQLNEINIYSHKETM
                     PQQMPVSYTIITPNVINGAQFNTIRDLSIYVPNFFIPDYGSAMSTAPYIRGVGSRSTG
                     QSMALYVDNVPYFEKTTFDFDFYDIMQIDVLRGSQGTLYGRNAMGGVVNIYTLSPLIY
                     QGTKLSVAGGNYGSVRAQAAHYAKFNSKMGLSLSGYYNHRNG"
     assembly_gap    1323..1422
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1423..3134
                     /locus_tag="P5236_03845"
     CDS             <1423..3134
                     /locus_tag="P5236_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRU63525.1"
                     /translation="FNSKMGLSLSGYYNHRNGFFTNTYTGKDADDKTSAGGRAKFDWQ
                     INPQWKMQYILNYDYVDQTAFPYGLYDKTTEEVANPNFNDPGAYNRSMVTNSLFIEHQ
                     NDKILFSSSTSHQYFSDDMKMDQDFTPLSFFSLEQIQKQHAVNEEFTLKSNTKNNYQW
                     SFGLTSFYQKLDMNAPVAFKKDAITQILQPAFDKAIAQGAPQMIITSEQMDIPGIYNT
                     ETYGGALFHQSTYNNLFVKGFSITAGLRGDFEKVKLDYNTNTSLNINMKMGNTPRPVT
                     LADTLKGKASKPFSEVLPKVALKYAWNEHQYIYGNVSRGYKAGGFNVQMFSDLMQKDL
                     MNTGNPNAPKIDVRKSILYYPEYSWNYEVGFQGTIWKNRIKTQISLFYMDIDSIQLTK
                     FVPSGNGRMITNAGKAKSKGIEFSTQANLGKGFSATINYGYAHAIFTNYIDSVAMNGN
                     FQQVDYKGKYVPYAPQQTLCLGGSYVHLFRHAFIDQFMANAQFLGAGKIYWNEANTLS
                     QKFYTLVNAKVGIRKGDIGLDVWVKNLFNTSYNAFYFESFGNSFFQKGNPLQCGMTLK
                     LEL"
     gene            complement(3357..3461)
                     /locus_tag="P5236_03850"
                     /pseudo
     CDS             complement(3357..3461)
                     /locus_tag="P5236_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774287.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT         1122 a          537 c          570 g         1132 t
ORIGIN      
        1 tttatttata gccttgtttc tgtaaacatt ttataatcaa atagtaaata atagaaaggc
       61 aattttatta tttgtcaaaa tgtcttttca aaaattttgt gtaagtttgc actgcgattt
      121 tataaattgc ctttttagct cagttggtag agcaattcat tcgtaatgaa taggtctggg
      181 gttcgagtcc ccaaaaaggc tcgaattttt cgttgaaaaa gttattaaaa tgttgatgtt
      241 cgacaacata ttgtttgcta cttttattcc acaaatttta atgttggtgg gatatttgtt
      301 ctgtctgctg gcaccgaagg caactacaaa ctcaacaact ttttcaattt ctgaatcatc
      361 aaaaaccatc agctattatt cttctgaaaa tcaaacttta aacaactcta cttttgttgt
      421 tgattttcat tctgatgttc cttgtaatat tgagaataat aaaaacctca ctattccaat
      481 ttgcaaatgc gaaatcagac ttcctatcca aaaggtgttt tttaaaattt ctgaaaaatt
      541 ttctttcaaa cttttctgcc gaccacctcc tgttttctgc tgattttcag tgatttttaa
      601 aaccttttct attcattatt cttatttcaa ataaataggg aatttacttt ccactatttt
      661 gaattttaat ttattggaga ttcaattcat tttaaacaat attaaacata aactaaaatg
      721 aaaaaaaatt taatagccgt tgtgctgtta tttcctctat ctttatatgc tgaagaagag
      781 caaaaaaatg attctttgcg taccattcag ctcaacgaaa taaatatata ttctcacaaa
      841 gaaacaatgc cacagcaaat gccagtttct tataccatca ttactcccaa tgtaataaat
      901 ggagctcaat ttaatacaat aagagactta agcatatatg tacccaattt ctttattccc
      961 gattatggat cagccatgtc aaccgctcca tacattcgtg gagttggttc gcgaagcaca
     1021 ggacaatcaa tggcgctata tgtggacaat gtcccttatt ttgaaaagac tacttttgat
     1081 tttgattttt acgacattat gcaaattgat gtgctccgtg gttcacaagg tacgctttat
     1141 ggacgaaatg ctatgggtgg agttgttaat atttatacac tgtctccact tatttatcaa
     1201 ggaacaaaat tgtcagttgc aggaggaaac tatggatcag ttagggctca agcagctcat
     1261 tatgccaaat ttaattcaaa aatgggtctt tcattaagtg gctattacaa tcatagaaat
     1321 ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaatttaat tcaaaaatgg
     1441 gtctttcatt aagtggctat tacaatcata gaaatggatt ttttaccaat acttatactg
     1501 gcaaagatgc tgatgacaaa acttcagccg gtggcagagc aaaatttgac tggcaaatca
     1561 atcctcaatg gaaaatgcag tatattctta attatgatta cgttgatcaa acggctttcc
     1621 cttatggatt atacgataaa actacagagg aagtagcaaa tccaaatttt aatgatccag
     1681 gggcttataa tcgttcaatg gtaacaaata gtttattcat tgaacatcaa aacgataaaa
     1741 ttttattcag ttcttccact tctcatcaat atttttctga tgatatgaaa atggatcaag
     1801 attttacacc tttgtcattt ttctcactgg aacaaattca aaagcaacat gctgtaaatg
     1861 aggagttcac acttaaatcg aatactaaaa ataattatca atggagtttt ggactcactt
     1921 ctttttatca gaaattagac atgaatgctc ctgttgcttt taaaaaagac gccataactc
     1981 aaattcttca accggctttt gataaagcta ttgcacaagg tgcaccacaa atgataatca
     2041 ccagtgaaca aatggatatc cccggaattt ataatacgga aacgtatgga ggagctttgt
     2101 ttcatcaatc cacttacaat aatctgtttg taaaaggatt ttctataacg gctggtttgc
     2161 gtggcgattt tgaaaaagta aaactggatt acaataccaa tacaagcttg aatatcaata
     2221 tgaaaatggg aaatacaccc cgtccagtta ctttggctga cacattaaaa gggaaagcat
     2281 cgaagccatt ttcagaagta ttgcccaaag ttgctcttaa atatgcgtgg aacgaacatc
     2341 aatatattta tggcaatgtg tcacgtggtt acaaggcagg cggtttcaat gtacaaatgt
     2401 tttctgattt gatgcaaaaa gatttaatga atacaggaaa ccccaatgca ccaaaaattg
     2461 atgttcgaaa atcaatttta tattatcctg aatatagctg gaattatgaa gttggatttc
     2521 aaggaaccat ttggaaaaat cgtataaaaa cacaaatttc attattctac atggatattg
     2581 atagcatcca gttaactaaa ttcgttccaa gtgggaatgg gcgaatgata accaatgccg
     2641 gaaaagccaa aagtaaaggg attgaattct ccactcaagc aaatttaggg aaaggtttta
     2701 gtgctactat taattacggc tatgctcatg caatatttac aaattatatt gattcagtag
     2761 ccatgaatgg aaattttcaa caagtcgatt ataaaggtaa atacgtacct tatgcgccac
     2821 aacaaacgct ttgtttagga ggtagttatg tacatctttt tagacatgct tttatagatc
     2881 agtttatggc aaatgcacaa tttcttggag caggtaagat atactggaat gaagccaata
     2941 cgctttcaca aaaattttac acacttgtga atgcaaaagt tggtattaga aaaggagata
     3001 ttgggttgga tgtatgggta aaaaatcttt ttaataccag ttacaatgct ttttattttg
     3061 aatcgtttgg caattcattt ttccagaaag gcaatccttt gcaatgtggg atgactttaa
     3121 aattagagtt atgatagcaa aataaggaaa gttacttcca tataagaaac atttatttta
     3181 aaaaatattt ttaagaaggg tatccggaaa ctgaatgaag gatatccttt tttttgtttc
     3241 aagggaattg aattgttttt ctgatacaaa aatgccccgc agataggcga ggcgctaaat
     3301 gttttcacaa cggaataatc cttattgtga tttgctttca aattattaat gttgtagtcg
     3361 taaatggtgt agagtttttt cttgagattt tgcttgagga ggttgttttc gtcgatgctt
     3421 tcaatggccg acttgaggat ataggcttct tcttcggtga a
//
LOCUS       DAOTKS010000146         3454 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_22712_length_3454_cov_3.449838, whole genome shotgun sequence.
ACCESSION   DAOTKS010000146 DAOTKS010000000
VERSION     DAOTKS010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22712_length_3454_cov_3.449838"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="P5236_03855"
     CDS             <1..201
                     /locus_tag="P5236_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63526.1"
                     /translation="QTSISNIELLADNQEYTAIKISIINNKAFLFITLNSNFDKTSEH
                     TINLDNQKFQFKGNYYFSALTK"
     gene            213..557
                     /locus_tag="P5236_03860"
     CDS             213..557
                     /locus_tag="P5236_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRU63527.1"
                     /translation="MKTRSLNFLHASEIEWEITGEGVSRQILGYDGQIMMVKVHFKKG
                     AIGYIHEHFHSQTTYVASGKFEVTINDEKQILEAGDGYYIEPDAPHGAVCLEEGILID
                     VFSPMRMDFLKK"
     gene            562..2025
                     /locus_tag="P5236_03865"
     CDS             562..2025
                     /locus_tag="P5236_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRU63528.1"
                     /translation="MKIKGLRWWIIGLIVLITIINYLDRGTLNYMWVTNVEYTIVDQF
                     SNNLKTNEAIYLKDKNTYLLKNKKGKIIEQQATHLSQKKDGKVVVNREGIAYDLGIIE
                     KDTTPDEASKKAKGILGTITIFFMIAYGISQLFSGKMYDKIGTRRGFVISAAIWGLAD
                     ALTSLAKGKFSLTAFRIMLGLGEAGPWPGAVKSNAEWFPQKERAFAQGLFNAAASIGS
                     ILAPIVILMLYLLVGWRLTFLIVGSLGLIWIIPWLIINKKGPKEHPWITEKEREYILT
                     GQPEAQIVDERGKSWGELLSNKKNYSVILGRFFLDPIWWMFVTYLPLYLVEQFNLNIT
                     ELAFSAWVPYFGAMIGSLTGGWFSGYLMRHGKTVDKARKTAMLVGGLIIVPSVIASVM
                     ASNAIVAVILMAFVLGGFQFTMTNIQTLPSDLHCGKSVGSLAGLGGASAVLGTILAIL
                     FSAKIPSWPLLFGLLAALVPLSLGSIFLTVGKIEQIK"
     assembly_gap    2055..2154
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2155..2661
                     /locus_tag="P5236_03870"
     CDS             <2155..2661
                     /locus_tag="P5236_03870"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRU63529.1"
                     /translation="DKIDILVNVVGGIMGRKEITEQDENWYSALMDVNMKSCWLCTRE
                     VVPHMNQGGAIINFSSLAARDGGGPGASMYATAKGAVMTFTRSMAKELGPKGIRVNAV
                     APGTIATSFHDKFNTPENRERMKGIYPLRREGDADDVADLVLFLASSDSDYITGANID
                     INGGIYFS"
BASE COUNT         1119 a          476 c          614 g         1145 t
ORIGIN      
        1 caaacctcca tttcaaatat tgaactttta gctgataatc aggagtatac tgcaataaaa
       61 atttcaataa ttaacaacaa agcatttctt tttattactc ttaacagcaa ttttgataaa
      121 acaagtgaac ataccattaa cttggacaat caaaaatttc aatttaaagg aaattattat
      181 ttttcagcat taacaaaata aaatttaaaa atatgaaaac aagaagttta aattttttac
      241 atgcttctga aatagaatgg gaaataacgg gagaaggtgt ttcacgacaa attttaggat
      301 atgatggaca aatcatgatg gtaaaagttc attttaaaaa aggagcaatt ggttatattc
      361 acgaacattt tcattctcag acaacttatg tggcgagtgg aaaatttgaa gtaaccatta
      421 acgatgaaaa acaaattttg gaagcaggtg atggatatta tattgaacca gatgctccac
      481 atggagcggt atgtcttgaa gaaggaattt taattgatgt atttagtcct atgagaatgg
      541 atttcttaaa aaagtaagat aatgaaaata aaaggattac gttggtggat cattgggctg
      601 attgtgctca ttacgatcat aaattatctg gatagaggaa cattgaatta catgtgggta
      661 accaatgtag aatataccat tgttgaccaa ttttctaaca acctaaaaac aaatgaagct
      721 atttatttga aagataaaaa cacttattta ttaaagaaca aaaaaggtaa aataattgaa
      781 cagcaagcta ctcatttatc tcaaaaaaaa gatggtaaag tagttgtaaa cagagaagga
      841 atagcttacg atttaggtat tattgagaaa gacacaacac ccgatgaagc ctcaaaaaaa
      901 gcgaaaggca ttctgggaac tattacgata ttttttatga ttgcctatgg cattagccag
      961 ctattttcag gaaaaatgta cgataagatt ggaacacgtc gtggttttgt tatctctgcg
     1021 gctatttggg gattggcaga tgcgcttacc tcacttgcaa aaggaaaatt ttccctgaca
     1081 gcctttagga taatgctggg attaggagaa gctggtccct ggccaggagc tgtaaaaagt
     1141 aatgctgaat ggttccctca aaaagaaaga gcttttgcac aaggactctt taatgcagca
     1201 gcctcaattg gttctattct tgctcccatc gttattttaa tgttgtattt gttggtgggt
     1261 tggagattaa cgtttttgat tgtcggttcg ctgggtttga tttggattat tccatggctg
     1321 attatcaaca aaaaaggtcc taaagagcat ccttggatca ccgaaaaaga acgcgaatat
     1381 atcctcacag gtcaacctga agctcaaatt gttgatgaga gaggaaaaag ttggggagaa
     1441 ttactttcaa ataaaaaaaa ttattctgtt atacttggac gattttttct tgatcctatt
     1501 tggtggatgt ttgtaactta tctacctctt tatctggtgg aacaatttaa tctcaatatt
     1561 accgaattag ctttttcagc ttgggtaccc tactttggtg ctatgattgg aagcctgaca
     1621 gggggttggt tctctggata tttaatgcgt catggaaaaa cggtcgacaa agcacgtaaa
     1681 acagctatgt tggtcggtgg gttaatcatt gtaccttccg tcattgcttc tgtaatggca
     1741 tccaatgcga tagtggcagt aattttaatg gcatttgtac ttggaggatt tcaatttact
     1801 atgactaata ttcaaacact ccccagcgat ttacattgtg gaaaatcggt cggttctctg
     1861 gctggtctgg gaggcgcttc tgcggtacta ggtaccatat tagcaatatt attttctgct
     1921 aaaataccaa gttggccttt actgtttgga cttttagccg cattagttcc tttgtcatta
     1981 ggatcaatat ttttaactgt tgggaaaata gaacaaatta aataacaatt ttttaaataa
     2041 taaatattat gaaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngataaa
     2161 attgacattt tagttaatgt agtaggaggg attatgggca ggaaagaaat tacagaacag
     2221 gatgaaaatt ggtattctgc cttgatggat gttaatatga aaagctgctg gctttgtaca
     2281 cgtgaagtag tacctcatat gaatcaagga ggtgctataa taaatttttc atcgttagct
     2341 gcaagagacg gtggaggacc aggagcatca atgtatgcaa cagcaaaagg agcggtgatg
     2401 acatttactc gttccatggc aaaagaactg ggaccgaaag gtattcgtgt taatgcagtt
     2461 gctccaggaa caatagctac ctccttccat gacaaattca atacacctga aaatcgtgaa
     2521 cgaatgaaag gtatttatcc acttcgtcgt gaaggtgacg cagatgatgt agctgatttg
     2581 gtcttatttc tggcttccag tgattcagat tatattacag gggcaaatat tgatattaat
     2641 gggggaatat atttttctta atctgttatt cccaatttat ttttacaatc tgagttttga
     2701 atgaaaaata gtaatataaa gttgcaaaat agcgtaatta ttttgcaact ttataattga
     2761 aaatcaaata atttataaat aatatcttgt agataaattt ttaaattttt ttatattatc
     2821 actaacgatt tttgtaatga acaggagaat aaagaaattc aagaatcact tttcgcaatt
     2881 ctattctatt tttatttgca aatgaaagaa gaaattattc ttcttttaga ttttgtttta
     2941 tgaaccaatt cataagcctc gtataatttt ttatacccgt ttcagaaagt ctttagaacc
     3001 tttttggtcg ttttcaaagc ttgcttgtac taaaaaccta ttgatttata acctatagta
     3061 tgttacatcg aacgtatttt tagaaaagtc aattctaaca aataaaaaaa aatttttcat
     3121 acttttgtat tttataggtt ttaaatttgg aattaaccga taaaaactgc tttgtactaa
     3181 ggcccttaat gggcttttaa tcttaatatt ctatttggta ttttgcagtt tgattcttaa
     3241 aagtcttgta ccctcacgta gaattaaatt tctatgtctc tacatagttc acttttaaga
     3301 atatacggtt aatttcttct tttttaccgg attttccttt caaaagtggt atattttata
     3361 tataacaaat tgattattat ttgtttaata tgttttttta aaataatttc ttgcttacag
     3421 tgtaattatt agttgtttat cacatattga atat
//
LOCUS       DAOTKS010000147         3453 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_22722_length_3453_cov_4.032666, whole genome shotgun sequence.
ACCESSION   DAOTKS010000147 DAOTKS010000000
VERSION     DAOTKS010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22722_length_3453_cov_4.032666"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..639
                     /locus_tag="P5236_03875"
     CDS             127..639
                     /locus_tag="P5236_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRU63530.1"
                     /translation="MKYNPDNHHRRSIRLQGYDYSQPGIYFITLCTHNRECLFGKILN
                     GEMRLNEFGKMTQQCWLEIPNHFPHVQLDEYIIMPNHIHGIIVLNDIIGVQNIEPQRQ
                     NAYQHIIPGSIGSIIRGFKIGVTKGFKILKPDNTDIYVVWQRNFYEHIIRNNDELNRI
                     RKNIIDNPEK"
     gene            995..2791
                     /locus_tag="P5236_03880"
     CDS             995..2791
                     /locus_tag="P5236_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HRU63531.1"
                     /translation="MSTQRYSVNPQPVETLLTWVKSGEIAIPEIQRPFVWNSTKVRDL
                     LDSLYQGFPIGFLIVWRNPNIKLKDGTISKGKRILIDGQQRITALMASLLAREVLTKD
                     YDTVNIRIAFNPQEERFEVTNPAISKDVKWIDDISKMFSPNTSILKLVNDYCDKNPGL
                     SEDDIFSAIEKLKNIIHNNVGIIELADDLDIETVTEIFIRVNSAGLELSQADFAMSKI
                     AVNETYGGNILRKAIDYFCHLAVAPEFYDKIKKNDPVFASSDYFQAMQWLKDSNDDIY
                     DPTYTDMLRVAFTSQFNRGKLQDLVALISGRNFETKQYEEVIAEQSFAKLKAGVLAFI
                     NKSNFERFVMIIRSAGFITSNLIGGQNALNFAYIVYLLGKKEGLSSDNIEHLVRRWYV
                     MSILRGRYAGSPETAFDIDIRQIGTNGLKKYIDTVCDTELPESFWTGMLPQLMNTSSA
                     QSPYFLAYKAAQVKLGDKGFLSRDITVRDLILNRGDVHHIYPKEYLKKKGMQRGMYNQ
                     IANFVIAQSEINIVIGDKPPQNYIPEIIEQCNGGEKKYGGITDMEELRDNFNVHCLPE
                     AFLNGNIPEYEDFLEERRKLMAIKIKTWYEML"
BASE COUNT         1231 a          488 c          652 g         1082 t
ORIGIN      
        1 agaatgtttg tttttgatga atatatgaat ctgttcaata aataattttg gaaacaaacg
       61 tatgaatgaa cagcagaaca tagaattcaa atagcaatta cgaaaattac ggaaaggagg
      121 gaaagattga aatacaatcc agataaccac catcgccgtt ccatacgttt gcagggatac
      181 gattattccc aaccaggtat ttattttatt accctttgca cacataatcg tgaatgttta
      241 tttgggaaaa ttttaaatgg tgaaatgcgg ttaaatgaat ttggaaaaat gacacaacaa
      301 tgttggttgg aaataccaaa tcattttccg cacgtacaat tggatgaata tatcattatg
      361 ccaaatcata ttcatggaat aatcgttttg aatgatatta ttggggttca aaatattgaa
      421 ccccaacgcc aaaacgcata ccaacacatt ataccggggt caattggttc cattattcgc
      481 ggatttaaaa ttggcgttac caaagggttc aaaatattga aaccggacaa cacggatatt
      541 tacgttgttt ggcaacgaaa tttttacgaa cacattattc gaaataatga tgaattaaat
      601 cgaattcgga aaaatataat tgataatcct gaaaaatagg aaacagatga aatttatatt
      661 tgatattcct gttatttttt caaccgccca gccgattgtt tgggcgaaaa atttttctcc
      721 ccaacataat tatatattta cgtaattgtt aaaaaatcca aaagccaaca aatttctgaa
      781 ttttattatt tacaatttat ttttttattg gaagttaagg atcaagagtt tatttttgga
      841 taataaatta gagatggcag tacagtatcg tgaagggaat tttctactat tcaaaaatgg
      901 aattactatt caatagttta ttggcgatta taagtcaatt gtattaagat aaattcaaag
      961 gagttttctg tcttaaaaaa gagaaatatt tactatgtca acacaacgtt attcagtaaa
     1021 cccacaacca gtagaaaccc ttcttacatg ggtaaaatcg ggagaaattg caatacctga
     1081 aatacagcgc ccctttgtat ggaattctac aaaggttcgt gatcttttag attccctata
     1141 tcaaggattt ccaatagggt ttcttattgt gtggcgtaat cccaatatca agctgaaaga
     1201 tggaactata tctaaaggca agcgtattct aatcgatggt cagcaacgta ttactgcact
     1261 tatggcttca cttttagcta gggaagtact tacaaaggat tatgatacag taaatatccg
     1321 aatagctttt aatcctcagg aagagcgctt tgaagttacc aaccctgcta ttagtaagga
     1381 tgtaaagtgg atagatgata tttctaaaat gttttctcct aatacaagta ttttgaagtt
     1441 ggttaatgat tattgtgata aaaatcctgg tttatcagaa gatgacatct tttcggcaat
     1501 tgaaaagtta aaaaatataa tacataacaa tgttggaata attgagttgg ctgatgatct
     1561 tgatatagaa acagtaacag agatatttat ccgtgtaaat tcggctggat tggaattgtc
     1621 gcaggcggat tttgccatgt caaaaatagc ggtaaacgaa acctatggag ggaatatttt
     1681 acgaaaggct attgattatt tttgccatct tgctgttgcc cctgaatttt acgataaaat
     1741 caaaaaaaat gatccagttt ttgcaagttc agattatttt caggctatgc aatggttaaa
     1801 agatagtaat gatgatattt atgatccaac ttatacggat atgttacgtg tagctttcac
     1861 ttcacaattt aatcgaggca aactacaaga tcttgttgcg ttaatttcag gtcgaaattt
     1921 tgaaacaaag caatatgaag aagttattgc agagcaatca tttgcaaaac taaaagcagg
     1981 agtattggca tttattaaca agagtaattt tgagcgtttc gtaatgataa tacgttctgc
     2041 tggtttcata accagtaatc taattggagg acaaaatgcc ttaaattttg catatatagt
     2101 gtatttattg ggtaaaaagg aagggttatc ttctgataat atagagcatt tggttcgacg
     2161 atggtatgta atgtcaattc tacgcgggcg ttatgcaggt agtcctgaaa ctgcttttga
     2221 tatagatata cgtcaaatag gaacaaatgg tttaaagaaa tatattgata ccgtttgtga
     2281 tactgaattg cctgaaagtt tttggacagg aatgctccct cagttaatga atacttcttc
     2341 tgcacaaagt ccctatttcc ttgcatataa agctgctcag gtcaaattgg gtgataaagg
     2401 atttctttca cgggatatta cagtacgaga tttgatatta aatagagggg acgtgcatca
     2461 tatttatcca aaggaatact taaagaaaaa gggtatgcaa cgcggtatgt ataaccagat
     2521 tgctaatttt gttattgctc agagtgaaat aaatattgtt attggtgata aacctccaca
     2581 aaattatatc cctgagatta ttgaacaatg caatggtggt gaaaaaaaat atggtggaat
     2641 tactgatatg gaagaattaa gggataattt taatgtccat tgtttaccag aggctttttt
     2701 gaatggtaat attccggaat atgaggattt ccttgaagaa cgaagaaagt taatggctat
     2761 caagataaaa acatggtatg aaatgttata agaaagcata tacccgatga cagtctactc
     2821 atatatagaa attgtatatt tatgttgttt tgatactttg catttaacct tctcgtaaaa
     2881 tgaatatcag ctttcttaag agataaagtt atttgagaat agccatagaa agtggaggat
     2941 taacaaggta gtttataaat tcaaaaactt tataattgat tttagagtta ttaactagga
     3001 aacttgcaga tacaggaaag ttaatgaata ttttaaaata aatcattgac taatgggcag
     3061 ctaaaagtaa taaaagatta atgcgaaaat attcgttagg aataggaaga attcaaagca
     3121 gtggtttcga tacgagctat cgctcacttc agcggctgtc tagaattcga tcgctaaggt
     3181 gcctgaatgg cgtatcgagg caatggacta attcgaccac gagtgaattc atgggcaaaa
     3241 caatacagat aacgcatagg ataccatcat tatgaactca acctccaacg acatactatc
     3301 agaaggcttc agaatgacag aacccagagc gctgtgggag gtggtgaaat gagaaaaaaa
     3361 attaaatttg actaatattg atgaatcaaa aggcttatat tagataaagg gacaatcaac
     3421 catgaaatta accagtatat tgaaacaaaa agg
//
LOCUS       DAOTKS010000148         3444 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_22778_length_3444_cov_4.174388, whole genome shotgun sequence.
ACCESSION   DAOTKS010000148 DAOTKS010000000
VERSION     DAOTKS010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22778_length_3444_cov_4.174388"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="P5236_03885"
     CDS             <1..983
                     /locus_tag="P5236_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HRU63532.1"
                     /translation="IFSLMNFYLPQRGIASMHCSANVGEEGDVAIFFGLSGTGKTTLS
                     ADPKRYLIGDDEHGWDDHGIFNYEGGCYAKVIDLSKENEPDIWGAIRRDALLENVTVL
                     PDGTPDFSKAASKTENTRVSYPIYYINKIVLPSKAGHASKIIYLSADAYGVLPPVSIL
                     DEKSAQYHFLCGYTSKLAGTERGINEPVPSFSPAFGEAFLTLHPTIYAKVLAEKMKKH
                     NAKAYLVNTGWNGTGKRISIKDTRAIIDAIIDGSIEKAPTTHIPIMNLTVPTSLNNVD
                     PKILDPRNTYANSGEWEEKAKSLAKQYIKYFEQYCDNVDARVLLASGPQL"
     gene            1101..1820
                     /locus_tag="P5236_03890"
     CDS             1101..1820
                     /locus_tag="P5236_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HRU63533.1"
                     /translation="MKIDICFSPALYPYYQTSEARMVVMVDVFRASTTICAAFAAGAN
                     AIIPVATIEEALRYKNQGWIVGAERNVEKCNFADAGNSPFEYTPEMVKGKEIVFTTTN
                     GTQAINSASSEWEVIIGCFSNLTAVTNFCLANKKEVLVLCAGWNNRFNLEDAVFGGAL
                     AERLIKEGNFEPCSDAVITAQSLWQLAGNDPENFLKNTEHAHRLFSHHLEADYYFCLQ
                     TDTTDVLPYYDKTSKKLLIKK"
     gene            complement(2064..2777)
                     /locus_tag="P5236_03895"
     CDS             complement(2064..2777)
                     /locus_tag="P5236_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_036612898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRU63534.1"
                     /translation="MSNPYFKFKKFTVFQDKCAMKVGVDGVLLGAWTPLENSMHILEV
                     GTGTGLISMMLAQRSNAFIDAIDIDKQAILQAQQNINFSPWKNRIQLSEISFQEYVQK
                     TASKYDLIVSNPPYFVNSLKAPFVSRTQARHADTLSHSELIELGNQILKPAGKICLIL
                     PVKEGLKCYLHALELNMRCQQLVFVYPRIELPAKRLLLHFSKEDAVTRVSKLVIETTH
                     RHQYTPEFKALVSDFYLNL"
     gene            complement(2810..3280)
                     /locus_tag="P5236_03900"
     CDS             complement(2810..3280)
                     /locus_tag="P5236_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63535.1"
                     /translation="MKAKNYICIIILMLGFVNYLQAQLPETAVENSPEQEAAKQTARL
                     EQELNLTLEQTKKIYEINLRYAKQRQLCNKRSEVMELIKKKDVEIQQVLSGDQYQQLL
                     NKRNERSTMQVPTTEGIRNITPSYRPINERKSVESSPSNRMKQKNTSSKPSPRK"
BASE COUNT         1107 a          592 c          608 g         1137 t
ORIGIN      
        1 gtattttttc tttaatgaat ttctatcttc ctcaaagagg gattgcttcc atgcactgtt
       61 ctgcaaacgt aggagaagag ggagatgtag ctattttctt tggcctttct ggtactggaa
      121 aaacaactct ttctgctgac ccaaaacgtt atctgattgg cgacgatgaa cacggttggg
      181 atgatcacgg catctttaat tatgaaggtg gctgctatgc aaaagttatt gacttgagca
      241 aagaaaatga acccgatatt tggggagcta tcagacgaga tgccttgctg gaaaatgtaa
      301 cggttttgcc tgacgggacg cctgattttt ctaaagctgc atcaaaaact gaaaatactc
      361 gtgtctctta tccaatttat tatattaaca agattgtgtt accttcaaaa gctggacacg
      421 caagtaaaat tatttatctt tcggctgatg cttatggagt tttgcctcct gtttctattc
      481 tggatgaaaa atcagctcaa taccatttcc tttgtggata tacttcaaaa ttagctggta
      541 ctgaaagagg tattaatgaa cctgttcctt ctttctcacc tgcttttggt gaagcttttc
      601 tcacacttca tcctactatt tatgctaaag tgttagctga aaaaatgaag aagcacaatg
      661 ccaaggctta tctggtaaat actggctgga atggtaccgg gaaacgtatt tcaatcaagg
      721 atacgcgtgc tattattgat gccatcatcg atggctcaat cgaaaaagct ccaactaccc
      781 atattcctat tatgaatcta accgttccaa cttcattaaa caatgtagat ccaaaaattc
      841 tggatccaag aaatacatat gccaattcag gagaatggga agaaaaagca aaatcgttgg
      901 ctaaacagta tattaaatat tttgaacaat attgtgataa tgtagatgct cgtgttttgc
      961 ttgcttcagg tccacaatta taatttttca caataaaaat ccactgataa agaatgagcc
     1021 ctttaaaaca aagactcatt ctttttcatt tatttcttga tgaaagttga gaaaagataa
     1081 ttaaacaaaa aaaaattcta atgaaaatag atatttgttt ttctcctgct ttatatcctt
     1141 attaccaaac gtcagaggca agaatggtag taatggtaga tgtttttaga gcttctacta
     1201 cgatatgtgc tgcttttgct gctggtgcca atgccattat acccgtggca actattgaag
     1261 aagctttgcg atataaaaat caaggctgga tagtaggagc tgaacgaaat gtagaaaaat
     1321 gtaattttgc cgatgcaggc aattcgccgt ttgaatacac acctgaaatg gtaaaaggca
     1381 aagaaattgt ttttactacc acgaatggga cacaggcaat taattcagct tccagcgaat
     1441 gggaagtaat aattggttgt ttttctaacc taacagcagt tacaaacttt tgtctggcaa
     1501 ataaaaaaga ggtgttggtt ctttgtgctg gatggaacaa tcgctttaat ctcgaagatg
     1561 ctgtttttgg aggagctctg gctgaacgat tgataaaaga aggaaatttt gagccttgct
     1621 cagatgcagt gataacagct caatcccttt ggcaactggc tggtaatgat cctgaaaact
     1681 ttttgaaaaa tacagaacac gctcaccgat tattttctca tcatttggaa gcagattatt
     1741 atttttgttt acaaacggat acaacagatg ttttaccgta ctatgataaa acttctaaaa
     1801 aattgcttat aaagaaataa aattataaaa aatttgattt ttatagcacc ttcctgaaac
     1861 ggtaaaacag caagaggctt ataattcacc ttcctcataa aaaatgatag aattttcgaa
     1921 attaccttat tcatcaggtt gtttttcttt tttctgaacg taattagcaa cactgtttaa
     1981 aattgttgct tgcaatgtat atcaaaaagt acgtagcact gatattttgg tagccactta
     2041 tgccagttaa tgaactggta tcattataaa ttcagataaa aatctgaaac caatgcttta
     2101 aattccggcg tatattgatg tcggtgagtg gtttcaatta ccaatttact tacacgagtc
     2161 acagcatctt cttttgaaaa gtgaagcaac aagcgttttg ctggaagttc aatacgagga
     2221 taaacaaaaa ccaactgctg acacctcatg tttaattcca aagcatgcaa ataacatttc
     2281 aatccttctt ttacaggtag aataaggcaa atcttgcccg ccggtttcaa aatttgatta
     2341 cccaattcaa tcaattctga atgagaaagt gtgtcagcat gccgtgcctg tgttcttgac
     2401 acaaaaggtg ctttcagtga gtttacaaaa taaggtggat ttgaaacaat taaatcatat
     2461 ttcgaagcag tcttttgaac atattcttga aaagagattt cagataactg gatacgattt
     2521 ttccacggtg aaaaattaat attttgttgt gcctgcaaaa tggcttgttt atcaatatca
     2581 atggcatcaa tgaaagcatt acttctttga gccagcatca ttgaaattaa acctgtccct
     2641 gtaccgactt ctaaaatatg catagaattt tcaagtggtg tccatgctcc taacaaaacg
     2701 ccatcaacac cgactttcat ggcacatttg tcctgaaaaa ctgtaaattt tttaaacttg
     2761 aaataaggat tagacatatg ttattgaaac gattttgcca ttctcttttt tactttcgag
     2821 gcgaaggctt tgaagaagta tttttttgct tcattctatt gctgggacta ctttcgacag
     2881 attttctctc atttatagga cgataagaag gcgtaatatt tcttattcct tcggtggtag
     2941 gtacttgcat ggtggaacgt tcattacgtt tgttcagaag ctgctgatat tgatctccac
     3001 ttaatacttg ctgaatttct acatcttttt tcttgatgag ttccattact tctgaacgtt
     3061 tattgcaaag ttgacgttgt ttggcatatc gaagattaat ttcataaatt ttttttgttt
     3121 gttcaagagt caaattcaat tcttgttcaa gcctggcagt ttgcttggcg gcttcttgct
     3181 caggtgaatt ttcaacagca gtttccggca attgagcttg caaataattt acaaaaccca
     3241 gcattaaaat tattatacaa atataatttt tagctttcat tccaaaaaaa gaaatttttt
     3301 aatagctaat ttggcaggtt ttaaacaaat actaaaagca ctcattcctt taataaaaaa
     3361 tacaacaacg attgtgccac aataaatgaa aacataaaaa attctcaatg gataaaaaaa
     3421 ggtttttcat ttaagttctt tata
//
LOCUS       DAOTKS010000149         3427 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_22909_length_3427_cov_4.395314, whole genome shotgun sequence.
ACCESSION   DAOTKS010000149 DAOTKS010000000
VERSION     DAOTKS010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22909_length_3427_cov_4.395314"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="P5236_03905"
     CDS             <1..642
                     /locus_tag="P5236_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63536.1"
                     /translation="ARYVRDVEVAGSSLVTPTLAFFIKNYYFPKNKFIMKKQFFILLG
                     CVAILFTSCLKNEESNYTPTISFLTYPITNHNDSLNIKLIDPSGTYQLDTIEVGDTVT
                     FLLGLNGYTNNLTSFYVQHTDSSATKIDFPPLENLNNIFSEPLSDYESGKFIFAYNYS
                     SIYFSFNYIAKKPSNNAAIIFTLSSDAQFSEGSGYNTVSFRLKTPIKQKISIQ"
     gene            774..1298
                     /locus_tag="P5236_03910"
     CDS             774..1298
                     /locus_tag="P5236_03910"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRU63537.1"
                     /translation="MERIFLIGYMGAGKSTVGELLAQALGYEFIDMDHEIEKRTQQTI
                     SNIFAEKGENEFRQLEYNCLHEIAQQNRVVISTGGGAPCFFNNLSFMKENGLTVYLKV
                     PAEQLKEKLEMSQHDKRPLISGKKGNDLLQYIKKGLAEREPFYQQATLVVTGTYEEML
                     NQILNYLSMHQKNN"
     gene            1546..>3427
                     /locus_tag="P5236_03915"
     CDS             1546..>3427
                     /locus_tag="P5236_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRU63538.1"
                     /translation="MNSFVHLHVHSHYSILDGMSSIPDLIEKAIAHNMPALALTDHGN
                     MFGIKEFYNYVKKKNQPILDKIASLTQQFQNIQLTDEEKKEIQLQIDREKKNLFKPII
                     GCETYVAHRSRHLKEIPEDRSGYHLILLAKNKTGYRNLCKIITLAYTEGMYYRPRIDK
                     ELLEKYREGLIATSACLGGEIHKKIESGNLAEAEEAILWYKKIFGDDFYLELQRHQTD
                     KPGADVTTYEKQKIQNAELIKLARKTNTKLIATNDVHFVEEEHADAHERLICLSTGTD
                     LDDPQRMRYTKQEWLKTPQEMAAIFEDIPEALNNTLEVAEKVEFYDIDSAPVMPLFPI
                     PEDFATEAEYRSKFTEEMLRQEFEESFDRLGGYNKVVRIKLEADYLTKLTLAGAEERY
                     GKNLPPEIEDRINYELKVIKTMGFPGYFLIVQDFIQAARDMGVAVGPGRGSAAGSVVA
                     YCLKITDIDPLKYDLLFERFLNPDRISMPDIDIDFDDDGRNQVLRWVTEKYGKNRVAH
                     IITFGTMAAKMAIKDVARVQKLPLSEAERLTKLIPDRLPDTPDGKKLKVNLSNCFEFV
                     PEIQVELDSSNKNIVETLKYAEMLEGTVRQTGVHACGVIIGADDLSNFIPLSTAEDKD
                     KKE"
BASE COUNT         1183 a          540 c          585 g         1119 t
ORIGIN      
        1 gcgcgctacg ttcgggacgt agaggtcgca ggttcgagtc ttgtcacccc gacattagcg
       61 ttttttatca aaaattatta tttccctaaa aataaattta ttatgaaaaa acaatttttt
      121 attttattgg gatgtgtggc tattttattt acatcctgtc taaaaaatga agagtcaaat
      181 tatactccta ctattagttt tttaacttat ccaattacca atcacaatga ctccttaaat
      241 attaaattaa ttgatccgag tggtacttat caactcgata ctatcgaagt aggcgataca
      301 gttacatttt tgcttgggtt aaatggttat acgaataatt taacctcctt ctatgtgcaa
      361 cacactgaca gcagtgctac taaaatagac tttccaccac ttgaaaatct gaataatatt
      421 ttttctgaac ccctttcaga ttatgaaagt gggaaattta tttttgcata taattatagt
      481 tcaatatatt tttcttttaa ttatatagct aaaaaaccaa gcaataatgc tgcaattatt
      541 tttactcttt cttctgatgc acaattttct gaaggaagtg gatataatac tgtttctttc
      601 agattaaaaa ctcccataaa gcaaaaaatt agtatccaat aattagtttt ttgaggatgt
      661 ttaaaaagga ctttttcaaa aaagaaaaat gtcctttttt tataacttta ttttaattat
      721 gttaattttg taagtttttg ttttctaaca tttttttgaa gcaatttttt tatatggaac
      781 gaattttttt aatcggttac atgggcgctg gaaaatctac tgtgggtgaa cttttagcac
      841 aagctcttgg ctatgaattt attgatatgg atcatgaaat tgaaaaaaga acacaacaaa
      901 ctatctctaa tatttttgct gagaaaggtg aaaatgaatt cagacaatta gaatataatt
      961 gtttacatga aatagctcag caaaatcgcg ttgttatttc aactggtggt ggcgcacctt
     1021 gcttttttaa taatttgtcc tttatgaagg agaatggttt gaccgtttat ttaaaagtgc
     1081 cagcagaaca acttaaagaa aaacttgaga tgtcacaaca tgacaaacgt cctcttattt
     1141 ctggcaaaaa aggtaatgat ttgttgcaat atattaaaaa agggctggct gaaagagaac
     1201 ctttttacca acaagccact ctcgtagtaa caggtaccta cgaagaaatg ttaaaccaaa
     1261 tcctgaatta tctttccatg catcaaaaaa acaattaaaa aattgaagta aaagacttcc
     1321 cttttcacat gttgctgttt taacaaacag ttgctttttc aactttttca gaaggaaaat
     1381 gaggagtatt tacatctatt tctgaaatgg tcaatcgaga actaaaataa ttcatcctct
     1441 ttcaattttt ttacattttt ttgatgcatt tttttattaa ctttatcctt caaaaatttt
     1501 ttagctattt tattcatatt caaagaccca ttagtttgat ttatcatgaa ttcttttgtt
     1561 catcttcatg ttcattctca ttattccatt cttgatggga tgtcatctat acctgatttg
     1621 attgaaaagg caatagccca caatatgcct gcccttgctt taacagatca tggcaatatg
     1681 tttggaatca aggaatttta caattatgta aagaaaaaaa atcagcctat tcttgataaa
     1741 attgcctctt taactcaaca atttcaaaac attcaactca cagacgaaga aaaaaaagaa
     1801 attcaacttc aaatagatcg ggaaaagaaa aatttgttta aacctattat tggatgtgaa
     1861 acctatgtag ctcatcgtag tcggcattta aaagaaatac ctgaagatag aagtggttat
     1921 catttaattc tgctggcaaa aaacaaaact ggttatcgca atctttgtaa aatcataaca
     1981 ttagcttata cagaaggaat gtattatcgt ccccgaattg ataaagaact tcttgaaaaa
     2041 tatagagaag gattaattgc aacgtcggct tgtctgggtg gagaaatcca taaaaaaata
     2101 gaaagtggaa atcttgcaga agctgaagaa gccattctct ggtacaaaaa aatttttgga
     2161 gatgattttt atttggaatt acaacgtcat caaacagata aacccggtgc cgatgttacc
     2221 acttatgaaa aacagaaaat ccaaaatgct gaattaatca aattggctcg aaaaaccaat
     2281 accaaactta tagctactaa cgatgtgcat tttgtggaag aagaacatgc tgatgcacac
     2341 gagcgtttga tttgtctaag tacgggtaca gatttagacg atccccaacg gatgcgttat
     2401 acaaaacaag aatggcttaa aacgccacaa gaaatggcag ctatttttga agatattccc
     2461 gaagcactca acaatacttt ggaagttgct gaaaaagtag aattttatga tattgattca
     2521 gcaccagtaa tgcctctttt ccctattcca gaagattttg ctaccgaagc tgaatatcgt
     2581 tccaaattta ctgaagaaat gttacgtcag gaatttgaag aaagttttga tcgtttgggt
     2641 ggctataata aagtagtgcg tatcaaactg gaagcggatt atttaaccaa acttacgctg
     2701 gcaggtgcag aggaacgtta tggaaaaaat ttacctccag aaattgaaga tcgtatcaat
     2761 tatgaattga aagtaataaa aacaatggga ttccctggtt attttcttat tgtacaagat
     2821 tttattcagg cagcaagaga tatgggtgtt gcagttggtc caggacgagg ttcagcagca
     2881 ggatcggtag tagcttattg tttgaaaata accgatattg acccactaaa atatgatttg
     2941 ctttttgaac gttttcttaa ccctgaccga atttctatgc ccgatattga tattgatttt
     3001 gatgacgatg gaagaaatca agtattacgt tgggttactg aaaaatatgg aaaaaacaga
     3061 gtggctcata ttattacttt tggaacgatg gcggcaaaaa tggctattaa agatgtggca
     3121 cgtgtccaga aattacctct ttcggaagct gaacgtctga caaaattaat tcctgacaga
     3181 ttacctgata ctcctgatgg gaaaaaatta aaagtaaact tatccaattg ttttgagttc
     3241 gttccagaaa ttcaagtgga gttagattcc tccaataaaa atattgtaga aaccctcaaa
     3301 tatgcggaaa tgctcgaagg cactgttcgt caaacaggag ttcatgcttg cggagtaatt
     3361 attggagccg acgatttaag caattttatc cctttaagta cagcagaaga taaagataaa
     3421 aaggaag
//
LOCUS       DAOTKS010000150         3423 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_22936_length_3423_cov_3.749109, whole genome shotgun sequence.
ACCESSION   DAOTKS010000150 DAOTKS010000000
VERSION     DAOTKS010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22936_length_3423_cov_3.749109"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..667
                     /locus_tag="P5236_03920"
     CDS             2..667
                     /locus_tag="P5236_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase"
                     /protein_id="HRU63539.1"
                     /translation="MVKTISEGRTRTNLLRKYEQSTLAYLVQRIPAWMSSDMLTAIGF
                     LGSVIVFLSFILAVYVNRYLLLVGVLGFVISWFGDSLDGRVAYYRHKPRKWYGFSLDI
                     TVDWLGVILMGAGFIVYAISPWKIIGFLFVGLYGWEMITALLRYKIINQYSIDSGAFG
                     PTEVRILISLILVLEVIFKNSIIYTSSLACLILLIMNILETKKILQLADERDKNERGG
                     EKI"
     gene            672..1091
                     /locus_tag="P5236_03925"
     CDS             672..1091
                     /locus_tag="P5236_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HRU63540.1"
                     /translation="MIERLFTFTKAQFSAMLGGAIDYTTMVFFTEVIHIHYTVSIVIG
                     GIVGALVNFTVNKGWTFRSKELPYKSTGLKQLLKFSLVVANSIFLKDVGTYLITTFVK
                     IDYKFSKIIVDLIVSIGFNYNLQKYWVFKKVPVNTTN"
     gene            complement(1172..1690)
                     /locus_tag="P5236_03930"
     CDS             complement(1172..1690)
                     /locus_tag="P5236_03930"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRU63541.1"
                     /translation="MKKFAILLLCLLPLKLIGQELKLGHINSQEILSLMPERSQIEKT
                     LGDLQNQWENELLKMQQEYYAKIKEFQDKQATMPESIKQARQSEIVEMEQRITTFRQT
                     ADSDLMKKQQELFAPVIEKVKKAINEVGAENNYTYIFDISTQVIVYQSPKSNDLTPLV
                     KKKLGLSSSSIK"
     gene            complement(1741..2256)
                     /locus_tag="P5236_03935"
     CDS             complement(1741..2256)
                     /locus_tag="P5236_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRU63542.1"
                     /translation="MKKLILTFCLFFSLSMAGIAQKFATVDMNYILKNIPAYEAANEQ
                     LNQISKKWQAEVEAQLKEVEAMYKNYQTELVFMTEEMKKKRENEIIAKEKAAQELKRT
                     YFGPEGELFKKRQSLMKPIQDEIFTAVQEISKEKNIDLVLDKSTSVNIIFSSPALDIS
                     DEILKQLGYSK"
     gene            complement(2330..>3423)
                     /locus_tag="P5236_03940"
     CDS             complement(2330..>3423)
                     /locus_tag="P5236_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRU63543.1"
                     /translation="NGQSYSSFDISFLEPWLGGKRPNSLSAMVYYSTQSAISNRYLNY
                     ISNLSSLYYGGYGGYGGYGDYYQTEVDPERYMRTFGASIGYGKRLNWPDDYFSFYSEL
                     SYQLFKLNNWYAGYFPMTGGTFNNLSLNLTLSRSSIDNPIFTRNGSLFSLGLKITPPY
                     SLFTGKDYSQPMTNEERYRFIEYHKWTFTGKTFTPLSYNNKLILMARAQFSYLGYFNK
                     YLKSPFETFYMGGDGMSAYTSYGTEYIAMRGYENGALTPYDVTTGVAAGYLYNKYTLE
                     LRYPLSLEQSATIYALGFVEAGNCFKSFSGPNGYNPFNLKRSAGIGVRIFLPMFGMMG
                     IDWGYGFDKVTGSTKPSGSQFHFVLGQEL"
BASE COUNT         1109 a          510 c          537 g         1267 t
ORIGIN      
        1 aattgtcaaa acaatttctg aaggaagaac gcgaaccaat ttattaagaa aatatgaaca
       61 gagtaccctt gcttatttag ttcaacgtat tccagcctgg atgagttctg acatgctcac
      121 tgctattggt tttttgggaa gtgtgattgt ttttttaagt tttatattag cggtgtatgt
      181 aaatagatat ttacttttag tcggggtatt aggatttgtc attagttggt ttggcgattc
      241 gcttgatgga agagttgctt attatcgaca taaaccaagg aaatggtatg gtttttcttt
      301 agatattacc gttgattggc tgggtgttat tttaatgggt gccggattta ttgtgtatgc
      361 aatttctccc tggaaaataa taggtttttt gttcgtaggg ctatatggtt gggaaatgat
      421 aacagcttta ctacgttaca aaattattaa ccaatattct attgattctg gagcatttgg
      481 accaactgaa gtgcgtattt taatttcact tattcttgtt cttgaagtaa tttttaaaaa
      541 ttcaattatt tatacttctt cattggcatg tttaatttta ttgataatga atattttaga
      601 aactaaaaaa atattacaat tagcagatga aagagacaaa aacgaaaggg gaggggaaaa
      661 aatttaataa tatgatcgaa aggcttttta cgtttactaa agctcaattt tcagctatgc
      721 ttggtggagc aattgattat actacgatgg tttttttcac tgaagtaatt catattcatt
      781 atactgtttc cattgttatt ggaggaattg taggagcatt ggtcaatttt actgtcaata
      841 aaggctggac ttttcgttct aaagaattac cttataaaag tactggacta aaacaattac
      901 tcaagttttc gctggtagtt gccaatagta tttttttaaa ggacgtaggt acttatttga
      961 ttactacttt tgtaaaaatt gattataaat tcagtaaaat tattgttgat ttaatagttt
     1021 ccattggatt taattataat ttacaaaaat attgggtatt taaaaaagtt ccagtaaaca
     1081 caactaatta attctgctat atgacaacaa agcctttgaa attaatcaaa agctttgttg
     1141 tttttactat atttaaaaat ttcttattat tttacttaat actgctacta cttaagccta
     1201 actttttctt taccaaaggt gttaaatcat tagacttagg ggattgatag acaatgactt
     1261 gtgtgcttat gtcaaatata taagtataat tattttctgc acccacttca ttgattgctt
     1321 ttttaacttt ttcaattaca ggagcgaaaa gttcttgttg ctttttcata aggtcactat
     1381 cagccgtttg tcgaaaggta gtaatacgtt gttccatttc aacaatttca ctttggcgag
     1441 cctgttttat actttcaggc attgtggctt gtttgtcttg aaattcttta attttagcat
     1501 aatattcttg ttgcattttc aataattcat tttcccattg attttgcaaa tcacctaaag
     1561 ttttttcaat ctgtgatctt tcaggcatta aggaaagaat ttcttgagag ttaatatgac
     1621 ctaattttaa ttcttgtcca attaatttta gaggaagaag gcagagtaac aaaatagcaa
     1681 atttcttcat gatgtctttg tttaaaaatt aatataaaat tagcttacaa atataggaaa
     1741 ttactttgaa tatcccaatt gtttaagtat ttcatcacta atatctaaag caggagaaga
     1801 aaaaataata tttacagaag tactcttgtc aagaactaaa tctatatttt tttctttgct
     1861 gatttcctga acagcagtga atatctcatc ttgaataggt ttcattaaac tttgtcgttt
     1921 tttaaaaagt tccccttcag gaccaaaata agtacgtttt aattcttgtg ctgctttttc
     1981 ttttgcaatg atttcatttt cacgtttttt cttcatttct tctgtcataa aaactaattc
     2041 agtctgatag tttttataca ttgcttctac ttcttttaat tgtgcttcta cttcggcttg
     2101 ccattttttt gaaatttgat tcaattgttc attagcggct tcataagcag gaatattttt
     2161 taaaatgtaa ttcatatcaa cagttgcaaa tttttgagct attcctgcca tcgacaaaga
     2221 aaagaataaa caaaaagtta atattaattt tttcatatcc aattttttaa aaattttatt
     2281 tctgaaaaac aaattccttg ccaaaattat taaaattttt tagttcaatt tataattcct
     2341 gtcctaaaac aaaatggaat tggctgccgc ttggttttgt tgaacctgtt actttatcaa
     2401 aaccatatcc ccaatctatt cccatcatgc caaacattgg caagaaaata cgtactccaa
     2461 ttccagccga acgttttaaa ttaaatggat tataaccatt cggtccagaa aagcttttaa
     2521 aacaattacc tgcctctaca aatcccaagg cataaatagt tgctgattgt tctaatgaga
     2581 gaggatatcg taattctaat gtatatttat tatacaaata tcctgcagcg actcctgtgg
     2641 taacgtcgta aggagttaag gctccatttt catatcctcg catggctata tattctgttc
     2701 cataactggt ataagccgac attccatcac ctcccatata aaatgtttcg aaaggagatt
     2761 taagatattt attgaaataa ccaagataag aaaattgggc tcttgccatc aatatcaatt
     2821 tattattata tgaaagtgga gtgaaagttt tgcctgtaaa tgtccactta tgatattcaa
     2881 taaatctgta gcgttcttca tttgtcattg gttgtgaata atccttgcct gtaaaaagtg
     2941 aatagggagg agtaattttt aaacctaagg aaaataaaga accattacga gtaaaaatgg
     3001 gattatcaat agaagaacgt gataatgtta agtttaagct aagattgtta aatgttcctc
     3061 ctgtcatggg aaaataacca gcataccaat tatttaattt aaataattga taagataatt
     3121 cactataaaa gctaaaataa tcatctggcc agtttaaacg tttaccataa cctatcgaag
     3181 ctccaaaggt gcgcatatat ctttcagggt caacttctgt ttgataataa tcaccataac
     3241 ctccataacc tccataacct ccatagtaaa gacttgataa gttgctgata tagttcaaat
     3301 aacgattact tatggctgat tgtgtagaat aatagaccat agcagaaagt gaattaggac
     3361 gtttccctcc taaccagggc tccaaaaatg aaatatcaaa tgagctgtaa gattgtccgt
     3421 tgg
//
LOCUS       DAOTKS010000151         3416 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_22989_length_3416_cov_2.791431, whole genome shotgun sequence.
ACCESSION   DAOTKS010000151 DAOTKS010000000
VERSION     DAOTKS010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22989_length_3416_cov_2.791431"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="P5236_03945"
     CDS             complement(<1..910)
                     /locus_tag="P5236_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HRU63544.1"
                     /translation="MSKKIVTFGEIMLRLATPGYERFCQASQLIATFGGGEANVAVSL
                     ANYGMQTEFVTRLPQNDIAESCIMELRKHNVGTNYIVRGGERMGIYFLETGAVARGSK
                     VIYDRSHSAIAEIQPGMIEWREVLKDADWFHWTGITPAISQGAADTCLEAIQVANQMG
                     ITVSVDLNYRKNLWKYGKSASEVMPALVQGCDIILGNEEDAEKVFEIKPEGFDVTKTG
                     GDIEGAKFESVCKQLMEKFPRAKKVIITLRGSINANHNTWGGVLYSNGKLYQSRRYDI
                     THIVDRVGAGDSFMGGLIYGLLNYPND"
     gene            complement(1014..2420)
                     /gene="uxaC"
                     /locus_tag="P5236_03950"
     CDS             complement(1014..2420)
                     /gene="uxaC"
                     /locus_tag="P5236_03950"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HRU63545.1"
                     /translation="MKKFLDENFLLQNETAQQLYHKHAEKLPIIDYHCHLIPAMVAED
                     YQFKTLTEIWLGGDHYKWRAMRTNGVDEKYCTGKDTSDWEKFEKWAETVPYTVRNPLY
                     HWTHLELKRAFGIEKLLSPKTAREIYEECTAKLQTKEYSAKNLMRKFNVEVVCTTDDP
                     VDSLEYHAKVKKEGFEIKMLPAWRPDKAMAVENPTVFRAYIEKLGQTADVNIVEFDDL
                     ITALRKRHDFFSEMGCKLSDHGIEEFYAEDYTENEIKTIFNKVYSGKELTKEEILKFK
                     SAMMVIFAELDWEKGWVQQFHYGAIRNNNTRLFKLLGPDTGFDSIGTFTTAKAMSKFF
                     DRLDVQNKLTKTIIYNLNPADNEVVATMIGNFQDGSVPGKMQFGAAWWFLDQKDGIEK
                     QLNTLSVLGLLSRFVGMLTDSRSFLSYPRHEYFRRILCNLLGNDIENGLLPYQELDFI
                     GQMVENISYYNAKSYFNF"
     gene            2710..>3416
                     /locus_tag="P5236_03955"
     CDS             2710..>3416
                     /locus_tag="P5236_03955"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRU63546.1"
                     /translation="MDYSSKKIRIKDIAKFSGVSKGTVDRVLHNRKGVSEKSRQAVEK
                     VLNEMNYSPNVFARSLASKKLYRIFCLFPAYQKGDYWENIENGFEQAAADFENYNVEI
                     LIEYFNQFDSSSFTKVSQSILEKEPDAIIMAPVFRNETIQFVNQLAEQKILFSYIDSM
                     IEETNFLTYYGQHSFQSGYVAAKLLFQTLPENSTVIITRTKRRGGAVSNQTTNRYNGF
                     MKYCEEFQLEKKFNLLK"
BASE COUNT         1122 a          635 c          496 g         1163 t
ORIGIN      
        1 catcatttgg ataattaagt aaaccataaa taagtcctcc cataaatgaa tcacctgctc
       61 caacgcgatc tacaatgtgt gtaatatcat aacgtctaga ttgataaagt tttccattac
      121 tatacaaaac accaccccaa gtattatgat tggcattgat agagccccgt aaagtaataa
      181 ttactttttt tgctcgtgga aatttttcca ttaattgttt gcataccgat tcaaattttg
      241 ctccttcaat atcaccacct gttttggtta catcaaaacc ttctggctta atttcaaaaa
      301 ctttttctgc atcttcttca ttccctaaaa taatatcgca accttgaact aaagcgggca
      361 taacttctga ggcagatttt ccatacttcc agagattttt tcgataattg agatccaccg
      421 aaacggtaat tcccatttga ttggctactt gaatcgcttc gagacaggta tcggcagctc
      481 cttgtgaaat ggcaggcgtt attcctgtcc aatgaaacca atcagcatct ttcaatactt
      541 cccgccactc aatcatacct ggctgaattt cagcaatagc agaatgggaa cgatcataaa
      601 ttaccttact accacgagct actgctcctg tttccaaaaa ataaattccc attctctcgc
      661 ctccacgaac aatatagttt gtccctacat tgtgttttct caactccata atacaacttt
      721 cagcaatatc attttgaggt aaacgagtta caaattcagt ctgcatacca taatttgcta
      781 acgatactgc tacattggct tctccaccac cgaaagttgc aattaattgt gaagcctgac
      841 aaaatctttc ataaccagga gtggctaatc gaagcattat ttctccaaaa gtgacaattt
      901 ttttactcat cttttcttta gttttttaat agttaaatag tgaacgagta aacaaaaata
      961 atatttttta ttgttatata gatttcaatt tttttagata atcttttaat gtttcaaaag
     1021 ttgaaataag acttagcatt ataataacta atattttcaa ccatctgacc aataaaatct
     1081 aattcctgat aaggtaataa accattttca atatcattgc ctaacaaatt gcacaatata
     1141 cgacgaaaat attcatggcg tggataggaa agaaaactgc gtgaatcggt aagcattcca
     1201 acaaaacgac tcaataaacc taaaacagag agagtattca actgcttttc aattccgtct
     1261 ttttgatcga gaaaccacca tgccgcaccg aattgcattt ttcctgggac gctaccatcc
     1321 tgaaaattgc caatcatggt agccacaact tcattgtcgg caggattgag attataaata
     1381 attgttttag taagcttgtt ttgaacatca aggcggtcaa aaaactttga cattgctttt
     1441 gctgtagtaa atgttccgat ggaatcaaaa cccgtatcag gtcccaggag cttgaataat
     1501 cttgtattgt tgttacgtat ggcaccataa tgaaattgct gaacccaacc tttttcccaa
     1561 tcaagttcag caaaaataac catcatggct gatttgaatt ttaaaatctc ttcttttgtc
     1621 aattcttttc cactatatac tttattgaaa atagtcttga tttcattttc agtataatct
     1681 tctgcataaa attcttcaat gccatgatcg gaaagtttac aacccatttc ggaaaaaaaa
     1741 tcatgccgtt tacgtaaagc tgtaattaaa tcatcaaatt ctacaatatt gacatcagca
     1801 gtttgaccaa gtttttcaat ataagctctg aaaacggtag gattttcaac tgccattgct
     1861 ttatcaggac gccaggcagg aagcattttt atttcaaacc cttctttttt cactttggca
     1921 tgatattcaa gtgaatcgac aggatcatca gtggtacata caacttctac attaaattta
     1981 cgcatgagat tttttgcact gtattccttc gtttgcagtt tagcggtaca ttcctcataa
     2041 atttctctgg ctgtttttgg cgaaagtaat ttttcaatgc cgaatgctct tttcaattcg
     2101 agatgtgtcc agtgataaag cggatttcgc acggtataag gcactgtctc agcccatttt
     2161 tcaaattttt cccagtcact tgtatctttg cctgtacaat atttttcatc cactccattg
     2221 gtacgcattg ctctccattt ataatgatca ccacctaacc aaatttcagt taaagttttg
     2281 aattgataat cttcagctac catagctgga attaaatgac aatgataatc aataattggc
     2341 aacttttcag catgtttatg ataaagctgc tgagccgttt cattttgtaa gagaaaattt
     2401 tcatctaaaa actttttcat tttttattga ttttaaaaaa ttattttaaa cgtttatttt
     2461 ctaataaaaa atttctactg aaacttattt tggatataaa aataataagc aatcctttaa
     2521 aaaatgttat aataatgctt ctatttttca acttttaaga taaaaaacat taattttttt
     2581 aaataaaaaa cctattaaat ttttgtgtcc gtgcacgaag gtacaaaaaa ttttatattc
     2641 tcaaaaattt ctattatgtt tgtaaaaatt atttttgtgt acgtacacgt attataattt
     2701 ttaagaaaaa tggactattc ttcaaaaaaa attcgtatta aagatattgc taaattttca
     2761 ggggtttcca aaggaactgt tgatcgagtt ttacataacc gaaagggagt ttctgaaaag
     2821 agcagacagg ccgtagaaaa agtactgaac gaaatgaatt actcgcccaa tgtatttgct
     2881 cgttcattgg cttcgaaaaa actctaccgc attttttgcc ttttccctgc ttatcaaaaa
     2941 ggtgattatt gggaaaatat agaaaatggt tttgagcaag cagccgctga ttttgaaaac
     3001 tataatgtag aaattttgat tgaatacttt aatcaatttg atagttcttc ttttacgaaa
     3061 gtttctcaat caatacttga aaaagaacca gatgctatta tcatggctcc ggtttttaga
     3121 aatgaaacaa ttcaatttgt caatcaactg gcagagcaaa aaatactttt ttcttacatt
     3181 gattcaatga ttgaagaaac caatttttta acttactatg gtcaacattc cttccaaagc
     3241 ggttatgtag ctgccaaatt gctttttcaa acgctgcctg agaattcaac agtgattatt
     3301 acgcgaacca aaagacgtgg tggcgcagta tcaaatcaaa caaccaaccg ctataacgga
     3361 tttatgaaat attgtgaaga atttcaactg gagaaaaaat ttaatttatt aaaaat
//
LOCUS       DAOTKS010000152         3415 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_22996_length_3415_cov_3.519643, whole genome shotgun sequence.
ACCESSION   DAOTKS010000152 DAOTKS010000000
VERSION     DAOTKS010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22996_length_3415_cov_3.519643"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="P5236_03960"
     CDS             complement(<1..421)
                     /locus_tag="P5236_03960"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRU63547.1"
                     /translation="MNVFSQTFNVSGTVLSAEDNQPIIGANIWIKGVNKGTVSDINGR
                     FSIEVEKNQTLVISYLGFENVEQRITGPVSNLKIILQPSTVVLEEVVAVGYGTMKKRD
                     LTGAIASVTGEDLRKLPAATVDKALQGLSPGVTAIANS"
     gene            complement(825..1718)
                     /locus_tag="P5236_03965"
     CDS             complement(825..1718)
                     /locus_tag="P5236_03965"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRU63548.1"
                     /translation="MKKLFTKKALMLLCAFFMASALFSQQKRYVFVLGGNPTWNSSWI
                     NLRLDDVNRVLYVWPDGSSLSGTTPVGTGSLGQDGYIAFTVGNIGWWGCGYFVLPTSQ
                     MDLTDVTEDWTFHFAIRTDCAQDITVGLNGSSPDPSDPFITTKTEGKVILNRTNLPLA
                     QRDKTQWVEFNIPMSQLMSGGSNNLKYLAPLTDQNYLFFSGGNDAGSFIAWDNVYIGA
                     PDGGSGIFTPKAEKLAFSIDDDGNYLNVFNNNSNDEINIYSISGAHVLNSKVNRVNIS
                     NLSSGVYIVKSGNLVNKFTKK"
     gene            complement(1983..>3415)
                     /locus_tag="P5236_03970"
     CDS             complement(1983..>3415)
                     /locus_tag="P5236_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HRU63549.1"
                     /translation="NSVRLPMHYNLFTLPIEEEPDPYKNTWLSKGFELTDSLVSWCKA
                     NKMYVILDLHAAPGGQGKDANISDYDPTKPSLWESDLNKNKTIALWQRIAEHYKDEPN
                     IGGYDLLNEPNWTFENKDIHGKEDLLNKDIWDLYIEISKAIRKVDKKHILFIEGNGWA
                     NNFNGFPGKWDNNMVLSFHKYWNPNTQQSIEPFIQLRKKYDIPLWLGESGENSNKWFT
                     DCIRLVEKNHIGWSWWPLKKVSSVVNPFTIIAPSGYDQLLNYWKYGESKPSKDFAAKV
                     LFELTNNLKAENCSFNKGIIDAMFRQRFETSTLPYTKNFIPGRIYGVDYDLGYYNRAY
                     FDTDNENIMVNGKRSVGNRGNQYRNDGVDIIRCNDKNEYTNGYHLFAIEKGEWLKYTV
                     DVKEKGDYQIFARIKPTSHPVKFNFIFGKKKKKKKIEFSHSDTNPNDWQTIYCGNINL
                     KKGKQNLFISFESNDFEFNFLEFIKK"
BASE COUNT         1174 a          627 c          447 g         1167 t
ORIGIN      
        1 cactattagc aattgctgta acgccaggtg aaagtccttg caaagcttta tcaacagttg
       61 ccgcagggag tttacgtaaa tcctcacccg ttacagatgc tatagctcct gttaaatctc
      121 tttttttcat agtgccataa ccaacagcta caacctcttc taaaactaca gtagaaggtt
      181 gtaatataat tttcaaatta gaaactggcc cagtgatacg ttgctctaca ttttcaaagc
      241 ccaaataaga aatgactagt gtttgatttt tttctacttc aatactaaaa cgtccattaa
      301 tatcagagac tgttccttta ttaactcctt ttatccaaat attggcacca ataataggct
      361 ggttgtcttc agcagataaa acagtaccgc taacattaaa agtttgtgaa aaaacattca
      421 tgactaaagt tacccaaaga aaaagtaacg ttatcttttt tttcatgtgt aaaaattatt
      481 atattgtttt aattatcatc aaatgaaatt tatccttaac aaattggata aaaatttcta
      541 aattcccaat aagtttttaa attaggtaat aatttaattg gctatttgaa attactttta
      601 ttgaaaatct tttttattga aaaatgtttt atttttgaat ctactacttc aaaaaaatta
      661 atttaaatat tttttgattt ataaatcgtt gaatatcagg attgaatttt taaaaggtaa
      721 ttagagaatg aacttttaaa agcaggctta tttttaaatc gaaaaaacag cttgtctata
      781 catgaagcat agacaagctg tacaatgaaa caaatcaatt caaatcattt ttttgtaaat
      841 ttattcacta aattacccga cttcacaata tacacacctg aggataaatt ggaaatattt
      901 acacgattaa ctttggaatt caaaacatgg gctccagaaa tactataaat attaatttca
      961 tcattactat tgttattgaa gacattcaaa taattaccat catcgtcaat agaaaaggca
     1021 agtttttcag cttttggagt gaaaattcct gaaccaccat ctggtgctcc tatgtataca
     1081 ttatcccatg caataaagct acctgcatcg ttgccacctg agaaaaacaa ataattttga
     1141 tcagttaaag gtgctaaata tttgagatta ttcgagcctc cactcattaa ttgtgacatc
     1201 ggaatgttaa attctaccca ttgtgttttg tcacgttgtg ctaacggcaa attagtcctg
     1261 ttcagaataa cctttccttc tgttttagta gtaataaaag gatcactagg gtcaggagag
     1321 cttccattta atccgactgt aatatcctga gcacaatcag tacggatggc aaaatggaat
     1381 gtccaatctt cggtaacatc agttaaatcc atttgagaag taggcagaac gaaataaccg
     1441 caaccccacc atccaatgtt accaactgta aaagcaatat aaccatcctg gccgagtgag
     1501 cctgtaccca ctggtgttgt tccactcaaa gaagatccat caggccatac atataagacc
     1561 ctattaacat catcaagacg aagattaatc cacgaagagt tccatgttgg attaccacct
     1621 aatacaaata cataacgttt ctgttggcta aacaaagcac tagccataaa aaatgcacat
     1681 aaaagcatga gagctttttt cgtaaataat tttttcataa aacaattttt tttaatttgg
     1741 ttaataataa tgtttaacaa aaataataaa taaaaagaat tttaaaaaaa ttcatccaaa
     1801 aataatgact tattcgaatt cgttccaaaa aaattacctc acaaaaactc acacccctaa
     1861 aatttaattt tcatttttcc tcaacatcaa ataataaact tctcctttat ttttctaaaa
     1921 ttcaactaat taaataattt ctttgatttt tttaatttac ctttcactaa aaatgaaaag
     1981 aattatttct taataaattc caaaaaatta aattcaaaat cattgctttc aaaggaaata
     2041 aacaaatttt gcttaccttt ttttaaatta atatttccac aatagatagt ttgccaatca
     2101 tttggatttg tatcactatg tgaaaattca attttcttct tcttcttctt ctttccaaaa
     2161 ataaaattaa atttaactgg atgagaagtc ggtttaattc tggcaaatat ttgataatca
     2221 cctttctctt ttacatctac ggtatatttt agccactctc ctttttcaat tgcaaataaa
     2281 tgataaccgt ttgtatattc atttttatca ttacaacgga tgatatctac tccatcattt
     2341 cgatactgat taccacgatt gccaacagat cttttgccat ttaccatgat attttcatta
     2401 tccgtatcaa aatatgccct gttgtagtaa cctaaatcat aatctactcc ataaattctt
     2461 cccggaataa agtttttagt gtaaggtaaa gtgcttgttt caaatcgttg gcgaaacata
     2521 gcatctataa ttcctttgtt aaaggaacaa ttttcagctt tcaaattatt tgtcaattca
     2581 aataaaactt ttgctgcaaa atcctttgaa ggttttgatt ctccatattt ccaatagttc
     2641 aacaattgat cataacctga aggagcaata atggtaaagg gattaaccac agaagaaact
     2701 tttttaagcg gccaccacga ccagccaata tgatttttct ctaccagtcg aatgcaatct
     2761 gtaaaccatt tattggaatt ttcaccactt tcaccaagcc aaagaggaat atcatatttc
     2821 tttctcaact gtataaaagg ttcaatagat tgctgggtat tagggttcca atatttatgg
     2881 aaacttaaaa ccatgttgtt atcccatttt ccagggaaac cattaaaatt attagcccat
     2941 ccattacctt caataaatag aatatgtttt ttatctactt ttcgaatggc tttggaaatt
     3001 tctatataca aatcccaaat atctttattt aaaagatctt ctttgccatg aatatccttg
     3061 ttctcaaaag tccaattggg ctcattcaat aaatcatatc ctccaatatt gggctcatct
     3121 ttgtaatgtt ccgcaatcct ctgccaaaga gcgatggtct tatttttatt caaatcgctc
     3181 tcccacaatg aaggcttcgt tggatcatag tccgaaatat ttgcatcctt tccctgaccg
     3241 ccgggtgctg catgaagatc taaaataaca tacattttgt ttgccttgca ccaggaaacc
     3301 aagctatcgg tcaactcaaa accctttgaa agccaggtat ttttatacgg atccggctct
     3361 tcttcaattg gtaaggtgaa taaattataa tgcataggga gtcgtacact attga
//
LOCUS       DAOTKS010000153         3402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_23105_length_3402_cov_4.137735, whole genome shotgun sequence.
ACCESSION   DAOTKS010000153 DAOTKS010000000
VERSION     DAOTKS010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23105_length_3402_cov_4.137735"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..1968
                     /locus_tag="P5236_03975"
     CDS             139..1968
                     /locus_tag="P5236_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HRU63550.1"
                     /translation="MHYTFYDFLILIGSLGVFLYGMKVMSEGLQKVAGERLRAIMAAM
                     TKNRFSGVLTGLLITSIIQSSSATTVMVVSFVNAGLLTLYQAITVIMGANIGTTVTAW
                     VISLFGFKVNIASFAVPLIGLSIPFIFSSKSRRKSIGEFLIGFAFLFMGLEMLKNSVP
                     DIQSNPEILAFFSKYTNYGFGSVLLFLLIGTILTIIAQSSSAIMAITLIMCSKGWLSF
                     ELGAVMVLGENIGTTITANLAAIPANVSAKRAALAHLIFNVFGVIWILIFFYPFINLI
                     SYIITNFGPGDPNLLTSFTTSLDPETLKVISSDTTALTSEQLTLKQQLLQYQLATSYG
                     LSLFHTLFNLINTCLLIWFVNFIAKVVSFIIKQKDSDEEFQLKYISIGLLSTSELSIL
                     QAWKEIKVYVDRAQRMFQMVRELYYEKNEDEFVKKFSRIQKYENISDRMEIEIAHYLT
                     KVAEGRLSEKSKFEIQTMLRIISEIESIGDSCYNLARILMRKREDKAIYTPEMDENVN
                     RMMDLVEKALKEMREAIDNYQVSDQELAKAQNLENEINNLRDRLKFQNIDDVKEQKYD
                     YLASVTYMDIIAECEKMGDFIINIVEALNEATNKKDKIPLLQL"
     gene            complement(2156..2710)
                     /locus_tag="P5236_03980"
     CDS             complement(2156..2710)
                     /locus_tag="P5236_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRU63551.1"
                     /translation="MKEEIIISGFGGQGVLSMGKILAYSGLMQGMEVSWMPSYGPEQR
                     GGTANVTVILSDEKISSPVLNSYDTAIILNQPSLEKFESKVKPGGILIYDGNNFHKFP
                     TRKDINIYRIDAIEYAYAHDMAKTLNMIILGGYLKIHPIVKMENVLKGLEKSLPERYH
                     HLIPLNEKAIQTGMEIIQLEHKVE"
     gene            complement(2783..>3402)
                     /locus_tag="P5236_03985"
     CDS             complement(2783..>3402)
                     /locus_tag="P5236_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRU63552.1"
                     /translation="IQDKTIGIAPVGCAVFAYNYLDIDWQEAAHGRAPAVATAIKRLL
                     PDRYVFTYQGDGDLAAIGTAETIHACNRGENIVIIFINNAIYGMTGGQMAPTTLEGMV
                     TSTTPYGRNIALNGYPLNITNLLAQLPGTCYVTRQSVHTVATVRKTKKAIQKAFENSM
                     MNKGTSVVEVVSTCNSGWKMTPEQANEWMEKNMFPEYPLGDLKNI"
BASE COUNT         1126 a          558 c          558 g         1160 t
ORIGIN      
        1 atccataatt taaatttctt aataaaaatg tattttcatt tcaattgtaa atatacaact
       61 ttttttattt ctttgctttt aagaattcaa aaattgaata actttgtagt gaaatcttaa
      121 aaaaacaacc ataaagtaat gcattataca ttttatgatt ttttgattct gattggctcg
      181 ttgggcgtat tcctttatgg aatgaaggta atgagcgagg gattacaaaa agtagcaggc
      241 gaacgtcttc gtgccattat ggctgcaatg accaaaaacc gttttagtgg agttttaaca
      301 ggcttattga ttacctccat aattcaatct tcgtccgcaa caacagtaat ggtagtaagc
      361 tttgtaaatg caggtttact tacactatat caagctatta ctgtaattat gggggctaat
      421 attggtacaa cagttactgc atgggttatt tctttatttg gctttaaagt aaacattgct
      481 tcttttgcgg ttccccttat aggactttca atacctttta ttttttcttc aaaaagtaga
      541 cgcaaatcta taggcgaatt tttaatcgga tttgcgtttt tgtttatggg acttgaaatg
      601 ttaaaaaatt cagttccgga tattcaaagt aaccctgaaa ttttagcatt tttttccaaa
      661 tatactaact atggatttgg gtcagtttta ttatttcttt taataggtac cattttaact
      721 attattgcgc aatcttcaag tgctataatg gctattacct tgataatgtg cagcaaaggt
      781 tggctttcat ttgaactggg agcagtgatg gtgctcggag aaaatattgg tacaaccatt
      841 actgcaaatc tcgcagcgat accagctaat gtttcggcca aacgtgcagc actggctcat
      901 cttatattta acgtttttgg agtgatttgg atattaatat ttttctatcc ttttataaat
      961 ttaatttcct atatcattac taacttcggg cctggagatc caaacttatt aacctctttt
     1021 accacaagtt tagatcctga aactttaaaa gtaatttcat cagataccac agctctcact
     1081 tccgaacaac taacccttaa gcaacaattg cttcaatatc aactggcaac ttcctatgga
     1141 ttatctcttt ttcacacgct atttaatctt ataaatacat gccttttaat atggtttgtt
     1201 aattttattg ctaaggttgt atcgtttatc atcaaacaaa aagattcgga tgaagaattt
     1261 caattgaaat atatatcaat aggattgctt tcaacttctg aactttctat tcttcaagca
     1321 tggaaagaga ttaaagttta tgttgatcga gctcaaagaa tgtttcaaat ggtacgagaa
     1381 ttatattatg aaaaaaatga agatgaattt gttaaaaagt tcagcagaat tcaaaagtac
     1441 gaaaacatta gtgataggat ggaaatagaa atagctcact atttgacaaa ggtagcagaa
     1501 gggcgattga gcgaaaaaag taaatttgag atccaaacta tgttgcgaat tatttctgaa
     1561 attgaaagca taggagatag ttgttacaac ctcgccagaa tactcatgag aaagagagaa
     1621 gacaaagcaa tttatactcc tgaaatggat gagaatgtaa atagaatgat ggatttggtt
     1681 gaaaaggcat tgaaggaaat gagagaagct atagataact accaggttag cgatcaagaa
     1741 ttagctaaag ctcaaaatct tgaaaacgaa atcaataatc ttcgtgatcg gcttaaattt
     1801 caaaatattg atgatgtaaa agaacaaaaa tacgattact tagcaagtgt tacctatatg
     1861 gacataattg cagaatgcga aaaaatgggc gattttatta tcaatattgt tgaagcgttg
     1921 aacgaagcaa cgaataaaaa ggataaaatt ccattactcc aattataagc tgaattcgac
     1981 taattaataa gaatggaaaa attttaattt gaattatttt gttctcatct ttaccctaaa
     2041 aaattttcct aatggtctgg ataaattcat ttcgcatgat aacttgcgat aaaacaaaaa
     2101 cgccgattca tgaggaatta tcggcgataa ggagacttta tcaaaaatca actttttatt
     2161 caactttgtg ctccaattga ataatttcca ttcctgtttg gatggctttt tcattcaaag
     2221 gaattaaatg atgataacgt tcaggcagtg atttttccaa accttttaga acattttcca
     2281 tttttacaat ggggtgtatt tttaaataac cacctaaaat aatcatattt aatgttttag
     2341 ccatatcgtg agcgtaagca tattcaatag catcaatgcg ataaatgttg atgtcttttc
     2401 tggtagggaa tttatgaaaa ttgttgccat cataaataag aatgccacct ggtttcactt
     2461 tactttcaaa tttttctaac gaaggttgat ttaaaataat agcagtatca tatgaattga
     2521 gcacaggaga actgattttt tcatcgctca aaattacagt tacattggct gttccaccac
     2581 gttgttcagg tccgtaagaa ggcatccaac ttacttccat accctgcatc aaaccagaat
     2641 aagccaatat tttacccatt gaaagaaccc cttgtcctcc aaaaccagaa attattattt
     2701 cttctttcat aatttaaatg ttagcaatga aagtattact ttatcacttt caattattta
     2761 gtttattttt tgaattgaaa atttaaatat ttttcaaatc ccccagcgga tattcaggaa
     2821 acatattttt ctccatccat tcatttgcct gttcaggtgt cattttccat cctgaattac
     2881 aagtagaaac aacttcaaca acagatgttc ccttgttcat catagaattt tcaaaagctt
     2941 tttgaatagc tttttttgtt tttcgaaccg tagctaccgt atggacactt tgacgagtta
     3001 cgtaacatgt tccgggtaat tgagctaaca aattagtaat gttcaatgga taaccattta
     3061 aagcaatatt ccgtccataa ggtgtggtag aagtcaccat tccttcaagg gtagttggtg
     3121 ccatctgtcc acctgtcatg ccatagatag cgttattgat aaaaatgatt acaatatttt
     3181 ctcctcgatt gcaagcatgg attgtttctg ctgtaccaat ggctgctaaa tcaccatctc
     3241 cttgataagt aaatacataa cggtcaggaa gtaatctttt aatagctgtt gccactgctg
     3301 gtgcacgtcc atgagctgct tcttgccaat caatatcaag ataattgtaa gcaaagacag
     3361 cacaacctac aggagcaatt ccaatggttt tatcttgaat gt
//
LOCUS       DAOTKS010000154         3397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_23158_length_3397_cov_4.749551, whole genome shotgun sequence.
ACCESSION   DAOTKS010000154 DAOTKS010000000
VERSION     DAOTKS010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23158_length_3397_cov_4.749551"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            583..1689
                     /locus_tag="P5236_03990"
     CDS             583..1689
                     /locus_tag="P5236_03990"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRU63553.1"
                     /translation="MEDKFVTLAIRTLKRAEKIKNVLEENGIETLIQNVNLEHPELSV
                     GVRIRIKESDLPKALKIVEEVEKAWDEEKDEKEEKGLQILVPLDFSDLIPQTIDFAFY
                     FAQKLKAKIVFLNVYYSPPFTISSVTDDMNTYTFGDAELLRRIIKTANEEMAKMNNQI
                     NERVSKGELPNVNFRFELKEGIPEDQILDYCKKNRPVLVIMGTRGKKISSELIGSVTA
                     EVIEACVSPVLAIPAYVSLNSPAEIKRIAFLTNFDEKDLIAIDTTISLFGSENLEIYF
                     IHVSQKKEVWDEVMIGGIKEYFSKHYPELKTHYDLISSGDSLQLIDDYLAKNEIHLLA
                     FNSPRRNLIRRLFNPGLAYKMVLHTDTPLFVTHV"
     gene            complement(1738..2466)
                     /locus_tag="P5236_03995"
     CDS             complement(1738..2466)
                     /locus_tag="P5236_03995"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HRU63554.1"
                     /translation="MNVFYVPEPTLRNTLSEEESQHAVKVLRLKEGEEIVLIDGKGGY
                     YKAKITFPDAKHCEFEIIETIPDFGKLNYYLHIAIAPTKNMERFEWFVEKATEIGISE
                     ITPIICQFSERKVIKMERLEKIMIAAAKQSIRAYFPKLNPSCRFDDFLKKYQDSQQFI
                     AHCYSFIENSEQIQKLEKKKLKDVAQKSLRTIILIGPEGDFSKEEIKKAIAAGYIPIS
                     LGENRLRTETAGIIACHTIALINQ"
     gene            complement(2506..3390)
                     /gene="folD"
                     /locus_tag="P5236_04000"
     CDS             complement(2506..3390)
                     /gene="folD"
                     /locus_tag="P5236_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HRU63555.1"
                     /translation="MYTLLDGKLISEQIKKEIAEEVRQMIAAGKKRPHLAVIIVGHDG
                     GSEAYVAGKIKDCEICGFKSTLIRFEDDVTEKRLLEEVQHLNEDADVDGFIVQLPLPK
                     HISEQKIIEAIDHKKDVDGFHPINVGRLCIGLPCYVSATPMGIMELLKRYQIETSGKN
                     CVVIGRSNIVGKPIAMLMMQKKYPGDATVTVCHSRTKNLPEICRQADIIVAALGQPEF
                     LKGDMVKEGVIVIDVGTTRVPSTETRSGYKLKGDVAFNEVAPKASYITPVPGGVGLMT
                     RVSLMKNTLLAAKREIYY"
BASE COUNT         1101 a          610 c          545 g         1141 t
ORIGIN      
        1 atcgatcaac ttttcgattt atcggacgcc ggttggcaga ttatctattt aacgatggat
       61 gaccacatta gaaatttgtt cctcgaaaaa gcctcgcatc ggaataattt taaacagatc
      121 atgcttggat aaatcatcca aattagtttg tttgcaattg aaaaattgtt tttactttat
      181 tcagaaacat tcaacacctt gatccgtcta tcaagatagt ggacaacttg ttttgaagag
      241 actaaactat ttctcttttg ttcatcccct aatgaataag aatttctcac tttcaatgtc
      301 aaaaagtcgt cccatttttc ttaatataac ggtaatttaa aaataaaatc aataatttac
      361 ttttgtgaat tgattttttt tatcaataca tcatgaataa attaatgtgg atttttattc
      421 tcaacctcca aatttttacg gcgtcatttt tcatagaaaa aattaaaaaa gcaaagattg
      481 cctttatttc agaaaatata ttaattttac atttgaaaac gattagaaag ttttgaaaaa
      541 ataattaata tcgtaaatta cgaatttaaa atatttaata ctatggaaga taaatttgta
      601 acccttgcta ttcgcacttt aaaacgggct gaaaaaataa agaacgtttt ggaagaaaac
      661 ggaattgaaa cactcattca aaatgtaaat ctggaacatc ctgaattatc ggtaggtgtt
      721 agaatacgta tcaaagaaag cgatttacca aaagctttaa aaattgtaga agaagttgaa
      781 aaagcttggg atgaagaaaa agatgaaaaa gaagaaaaag gcttgcaaat tcttgttcca
      841 cttgattttt ccgatttgat tcctcaaact attgattttg ctttttattt tgcacagaag
      901 ttaaaagcaa aaattgtttt tttgaatgtt tattacagtc ctccttttac catttcttcc
      961 gttactgatg acatgaatac ctatacattt ggtgatgctg aattattgcg tcggattatc
     1021 aagacagcca atgaagaaat ggcaaaaatg aataaccaaa tcaatgaacg tgtctcaaaa
     1081 ggagaattac ccaatgtcaa ttttcgtttt gaattgaaag aaggcattcc tgaagatcaa
     1141 attctggatt actgtaaaaa aaaccgtcct gttttagtta ttatgggaac gcgaggcaaa
     1201 aaaatttcca gcgaattgat tggtagcgtt acagcagaag tcattgaagc ttgcgtttca
     1261 cctgttctgg ctattcctgc ctacgtatca ttgaattctc cggcagaaat caaaagaatt
     1321 gcttttttga caaattttga tgaaaaggac ttaattgcta tcgataccac tatttccctg
     1381 tttggatctg aaaatctgga aatatacttc attcatgttt ctcaaaaaaa agaagtatgg
     1441 gacgaggtaa tgattggagg cataaaggaa tatttctcga aacattatcc tgaactcaaa
     1501 acacattatg atttaataag ctcaggtgat tctttacaat tgattgatga ttatctggca
     1561 aaaaatgaaa ttcatcttct tgcttttaat tcaccgcgtc gaaatctcat cagaaggtta
     1621 ttcaatccag gtttggctta taaaatggta cttcatactg atacgccgtt atttgttact
     1681 cacgtgtaac tttctgtttt tttgaaaatc agtaggaaaa aaaagtaata aaacttttta
     1741 ttgattgatg agggcaatag tgtggcaggc aatgatacct gctgtttcgg tacgcaaacg
     1801 attttcaccg agggaaatcg gaatgtatcc tgcggcaatc gcttttttaa tttcttcttt
     1861 gctgaaatca ccttctggac caattaaaat aatggttctc aaagattttt gagcaacatc
     1921 ttttagcttc tttttttcaa gtttttgaat ttgttcactg ttctcgataa aagaatagca
     1981 atgtgcaata aactgctgtg aatcttgata tttcttcaaa aaatcatcaa aacgacacga
     2041 gggatttaat ttcgggaaat aagctctgat ggattgtttg gcagcagcaa tcatgatttt
     2101 ttccaagcgt tccattttaa taactttacg ttctgaaaat tgacaaatga tgggcgttat
     2161 ttcgctaatg ccaatttcag tcgctttctc gacaaaccac tcaaaacgtt ccatgttttt
     2221 ggttggagca atcgcaatat gcaagtaata atttaatttg ccaaagtcag gtatcgtttc
     2281 tataatttca aattcacaat gtttagcatc agggaatgta atttttgctt tgtaatagcc
     2341 tcctttacca tcaatcagaa cgatttcttc gccttccttt aaacgtaaca ctttgacggc
     2401 atgttgagat tcttcttcgg aaagagtatt tcttaacgtg ggttcaggaa cataaaaaac
     2461 attcatactt acattctttt actttttcgg aaaaaatgcc agaaatcaat aataaatttc
     2521 tcttttagcg gctaacagtg tatttttcat taacgaaacc cttgtcatca accctacccc
     2581 gccaggaaca ggcgtaatgt aagaagcttt aggagcaact tcgttaaaag ctacatctcc
     2641 tttgagcttg tatcctgacc tcgtttcagt agaaggaact cgcgttgttc ctacgtcaat
     2701 cactatcaca ccttctttca ccatgtcacc ttttaaaaat tctggctgac ctaaagcagc
     2761 gacaataata tcagcttggc gacagatttc tggcaaattc ttagtacggc tatgacaaac
     2821 tgttacagta gcatcgcccg gatatttttt ttgcatcatc aacatcgcaa ttggtttacc
     2881 aacaatgttg cttcgtccga taacaacaca atttttacca ctcgtttcaa tttgatagcg
     2941 ttttagtagt tccataattc ccatcggtgt agcagaaaca taacaaggca aaccgataca
     3001 taaacgaccc acattaatag gatggaaacc atccacatct tttttatgat caatagcttc
     3061 gataattttt tgttctgaaa tatgtttggg caaaggtaat tgaacaataa agccatccac
     3121 atcagcatct tcgtttaaat gctgcacttc ttccagcaaa cgtttttcag taacatcgtc
     3181 ctcaaatcga attaaagttg acttaaatcc gcaaatttcg caatctttaa ttttcccagc
     3241 tacatacgct tcactaccac cgtcgtgtcc tacaatgatt acagccagat gaggacgttt
     3301 tttaccagct gctatcattt gccgaacttc ttcagcaatt tcttttttta tttgttccga
     3361 aataagtttc ccatctaaaa gtgtgtacat aaattga
//
LOCUS       DAOTKS010000155         3396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_23165_length_3396_cov_3.043999, whole genome shotgun sequence.
ACCESSION   DAOTKS010000155 DAOTKS010000000
VERSION     DAOTKS010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23165_length_3396_cov_3.043999"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1891)
                     /locus_tag="P5236_04005"
     CDS             complement(<1..>1891)
                     /locus_tag="P5236_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRU63556.1"
                     /translation="RLGIPLLIGRDVIHGFKTIFPIPLGQAATFDPELVKNAAHIAAV
                     EASSVGIKWTFAPMVDIARDPRWGRIAEGCGEDPYLTSVMAIAMVKGFQGNGSKDPTS
                     IAACPKHFVGYGAAEGGRDYNSTFLPERLLRNVYLPPFEASAKAGAYTFMTSFNDNDG
                     VPSSGNEFILKQVLRKEWGFDGFVVSDWASIREMIAHGFAADEKEAALKAVNAGVNME
                     MVSMTYFNHLKELIKEGKVKQETIDDAVRNILRIKFRLGLFENPYVDVNQASVMYADT
                     HLAAAKRTATESVILLKNDNQTLPLDENIKTIAVVGPMADAPYEQMGTWVFDGDKAFT
                     KTPLNALKEKYGNKINIIFEPALAFSRDKNTDGIEKAVKAAAQADVVLAFVGEESILS
                     GEAHCLADLNLQGAQSELIQAVAKTGKPVITVIMAGRPLTIAKEAALSSAVLYSFHPG
                     TMGGPALIDLIFGKEIPSGKTPVTFPKTVGQIPIYYAHNSSGRPADGKETLIDEIPVE
                     AGQTSLGNRSFYLDAGSEPLYPFGYGLSYTTFEYDNLKISADILKKSDILKVSFDLKN
                     TGKYAATEVAQLYVQDKFGSVTRPVKELKRFARVTLQPGEKQTVNFEFPVEELAFWNI
                     DMKKVV"
     assembly_gap    1892..1901
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1902..2118)
                     /locus_tag="P5236_04010"
     CDS             complement(<1902..2118)
                     /locus_tag="P5236_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63557.1"
                     /translation="MAISPKNDPVEKKIDKLISRMTLEEKIGQMNQISSFGSVETMEE
                     FVKNGSIGSIINEIDPVNINALQRIAVN"
     gene            complement(2194..3252)
                     /locus_tag="P5236_04015"
     CDS             complement(2194..3252)
                     /locus_tag="P5236_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HRU63558.1"
                     /translation="MTSCSNNAPAVSSENIQIQILPSSLNFDYTASEQSILITSNADF
                     TVLSDQIWCNVSSSNINKGTDSLKISVAENTTNSKRNAIITFKSGNFEKKYSVSQDCH
                     LEINVPEGYRLVWQDEFNEYHASGEKPLLPNATKWWFETGGGGWGNNELERYIPGYEG
                     SDTCSVVSNGTLKIIAKKKGNEVISIRMNTSKSWTYGYFEARLKLPQGKGTWPAFWMM
                     PKNFSAWPDDGEIDIMEEVGYRPNWVSASIHCSAYNHKMGTQKTAEKFVSAAESDFHV
                     YAVEWTESFIKGYVDGENYFTFLNDGKKDKKTWPFNTPFYLKLNLAWGGNWGGAQGVD
                     ESKLPATYEIDYVRVFQK"
BASE COUNT          968 a          730 c          614 g         1074 t
ORIGIN      
        1 caaccacttt tttcatgtca atattccaga atgccaattc ttctacggga aattcaaagt
       61 taacggtttg tttttcaccc ggttgtaagg ttacgcgtgc aaaacgtttg agttctttta
      121 caggtctggt aaccgagccg aatttgtcct gaacataaag ttgagcaact tcagttgcgg
      181 catattttcc ggtatttttt aaatcaaaac taactttcag tatatctgat tttttcaaaa
      241 tatctgccga aatttttaag ttgtcgtatt caaatgttgt gtacgacaat ccatatccaa
      301 aaggataaag aggctcagaa cccgcatcca ggtaaaatga acgattgccg agcgacgttt
      361 gtccggcttc aaccggaatt tcatctatca gtgtttcttt gccatcggca ggccgtccag
      421 aactattgtg agcataataa atggggattt gtccaactgt ttttggaaaa gttacaggtg
      481 ttttaccact gggaatttct tttccaaaaa taaggtcaat caatgcaggg ccacccatag
      541 ttccgggatg aaacgaatac aaaactgccg acgacagagc tgcttctttt gctatggtca
      601 gaggtcgccc agccatgatt actgttataa cgggcttgcc tgttttagca acagcttgaa
      661 tcaactccga ttgtgctccc tgcaaattca aatctgccag gcaatgtgct tctcctgaaa
      721 ggatagattc ctctccaaca aaagccaaaa cgacgtcggc ttgtgcggct gctttcacgg
      781 ctttctcaat tccatcggta tttttatctc ggctgaaagc aagagcaggt tcaaaaatta
      841 tgttgatttt atttccgtat ttttctttta atgcgttcaa tggagttttg gtaaaagcct
      901 tatctccgtc gaacacccat gttcccattt gttcatacgg tgcatcagcc attggcccaa
      961 cgacggcaat ggtttttata ttttcatcca atggtaaggt ttggttgtcg tttttcaaaa
     1021 ggataaccga ttctgtagca gtacgcttgg ccgcagcgag atgggtatct gcatacatta
     1081 ctgaagcttg attcacatca acataaggat tttcaaacag gcccaaccga aatttgatac
     1141 gcaaaatgtt tcgtaccgca tcgtcgattg tttcttgttt tacttttcct tcttttataa
     1201 gttctttcaa atgattaaag taagtcatac tcaccatttc catattcact ccggcgttta
     1261 ctgcttttaa ggccgcttcc ttttcgtcgg ctgcaaaacc atgagcaatc atctctctaa
     1321 tggaagccca atcggaaacc acaaatccat caaatcccca ttccttgcgt aaaacctgtt
     1381 tcagaataaa ttcgtttccc gaagaaggaa cgccatcatt gtcattgaat gaggtcataa
     1441 aagtataagc accggctttt gcacttgctt cgaaaggagg aagatatacg ttgcgaagca
     1501 aacgttcggg caaaaaagtg gaattatagt cgcgtccccc ttcggcagct ccatagccaa
     1561 caaaatgttt gggacaggca gcgatagaag tcggatcttt tgacccgttt ccctgaaatc
     1621 cttttaccat agctattgcc atcacagaag taagatatgg atcttctccg catccttccg
     1681 caattcttcc ccaccgcggg tcgcgagcaa tatccaccat tggtgcaaaa gtccatttta
     1741 tacccactga ggaagcttcg acagctgcta tgtgggctgc attttttacc agttccggat
     1801 cgaaagttgc tgcttgacct aagggaatag gaaaaatagt tttaaaaccg tgaataacat
     1861 cccttccaat aagcagcgga attcccaagc gnnnnnnnnn ncgttgacag caattcgctg
     1921 aagcgcatta atgttgaccg gatcaatttc gttgataatg gaacctatac tgccattttt
     1981 cacaaattct tccattgttt ctactgaacc aaatgaactg atttgattca tttgtccaat
     2041 tttctcttca agtgtcatcc gggagatcaa tttatctatc tttttttcaa caggatcgtt
     2101 ctttggactg atagccatca aactggaact aaataaaaac aaacttgcag aacttgccat
     2161 taaaaatttt aaccacttca tattattatc tatttatttt tgaaacactc gaacataatc
     2221 aatctcataa gttgcaggaa gtttgctttc atctactccc tgagcacctc cccagtttcc
     2281 tccccatgca agattaagtt tcaaataaaa aggggtattg aaaggccacg tttttttgtc
     2341 cttttttcca tcattgagaa aggtaaaata attttctcca tccacatatc ctttaataaa
     2401 actttctgtc cattccacag cataaacgtg aaaatcggac tcggctgcag atacaaactt
     2461 ttcggctgtt ttttgagtac ccattttatg gttgtaagcc gaacaatgaa tcgaagccga
     2521 cacccaattg ggacgatagc caacttcttc cataatatcg atttccccat cgtcgggcca
     2581 agcagaaaag tttttgggca tcatccagaa tgcaggccag gttccctttc cctgaggcag
     2641 tttaagtctg gcttcaaagt aaccgtaagt ccagctttta gaagtattca ttcgtatgga
     2701 aattacctcg tttcctttct ttttggcaat aattttcaac gtcccatttg aaacgacaga
     2761 acaagtatca ctgccttcat atccgggaat atatctttca agttcattgt ttccccagcc
     2821 acctccgcca gtttcaaacc accatttagt agcattcggc aacaaaggtt tttctccgga
     2881 tgcatgatat tcgttaaatt catcctgcca aaccaaacga taaccttcag gcacattaat
     2941 ttccaaatgg cagtcctgac tgacgctgta ttttttttca aaattacccg atttgaaagt
     3001 aataatagcg ttgcgtttgc tatttgttgt attttcagca acggaaattt ttaacgaatc
     3061 tgttccttta tttatgtttg aagaagaaac gttgcaccat atctgatcgg ataaaaccgt
     3121 aaaatcggca ttggaagtta ttagaattga ttgttccgat gctgtgtaat caaaattcaa
     3181 agaagaaggc aaaatctgaa tctgaatatt ttcagatgac actgccgggg cattgttgct
     3241 acaacttgtc attaaaaaac ttaaacaaaa taaatatgtg agtgtcgatt ttcgcatgtt
     3301 aggcaaatag aatattgttt tgtgaagtga ataatttttt gtgtttatga aaaaaatatt
     3361 ttttatttta gctcaataat ttatttcatt aaattg
//
LOCUS       DAOTKS010000156         3394 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_23183_length_3394_cov_4.991015, whole genome shotgun sequence.
ACCESSION   DAOTKS010000156 DAOTKS010000000
VERSION     DAOTKS010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23183_length_3394_cov_4.991015"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="P5236_04020"
     CDS             <1..603
                     /locus_tag="P5236_04020"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRU63559.1"
                     /translation="YALGLLVGNDFLKNIKTIPGGKTNVDLIIKGFTTSMKGDSAIIP
                     LDSANNYLRNYFTAAQTKDSEKRKTAAIIFMEQNKKKEGIITTESGLQYEILKATEGP
                     KPANTDKVKVNYEGFLIDGTKFDSSIDRGEPIVFALNQVIPGWSEGLCLMSVGSKYKF
                     YIPYELAYGAQGAGNVIPPFSPLIFEVELLEINPDEENNQ"
     gene            627..1148
                     /locus_tag="P5236_04025"
     CDS             627..1148
                     /locus_tag="P5236_04025"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase
                     N-terminal domain-containing protein"
                     /protein_id="HRU63560.1"
                     /translation="MKKLSILSLIVVMTTFLMVSCKNKYEAKKSELSNQNDSLNYTLG
                     LVNGEGIKNYYLQNDSSDKPIEAFMNALDKAYKSKKTDKWYQLGYQIGTSLKFQESHG
                     LMADTTLAFNIKLVKQGIINALYNFDGGMSPQQAQEYLQKTMYELQAKKASEQALPSV
                     PKKDSTLDTLNTK"
     gene            1175..2038
                     /locus_tag="P5236_04030"
     CDS             1175..2038
                     /locus_tag="P5236_04030"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRU63561.1"
                     /translation="MKKINIFSVLIILIAFAIVSCNSNKPAKPVLKNQLDSLNYAFGV
                     ANGDGIKNYYLQGDSSDETIKSLIKGMNDGLKSKVEKEHLEISFLGTQIGSSLKEQEK
                     IGLMGDSTLKTDINLIKQGLVNGLRGSNIQMEPEEAKVYLQSTMQNRQAKQIEKEYAA
                     TKAAGELFLKENAKRPGVIITPSGLQYEIIKKGNGPIPTDSSKVKVHYHGTLIDGTVF
                     DSSVERKEPSVFYVNQVIKGWQEILKLMPVGSKYKIYVPQELAYGGQEQGLIKPYSPL
                     IFEIELLSIEK"
     gene            2249..3112
                     /locus_tag="P5236_04035"
     CDS             2249..3112
                     /locus_tag="P5236_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 family protein"
                     /protein_id="HRU63562.1"
                     /translation="MDKKTRKLLISVQRNEITEHFIYKRLAEGCKDQKNAEILHTISE
                     QEKQHAAFWQEKTNLKVKPNYWRITYTVFLARLLGLSFVLKQMEKREGTGSKLYNELS
                     NIIPETKFFAEEESIHEQQLLTMLDEERLQYVGSIVLGLNDALVELTGALAGFTLTLG
                     TTRTISLAGLVTGISAALSMAASDYLSSKAEGNKKAKKSAAYTGITYFFTVIFLILPF
                     LLLSSRFFALSLTLLLAVLIIFFFNYYISVAKELEFKSRFFEMCFISLGVAAFSFFIG
                     YILNSILGVNA"
BASE COUNT         1258 a          500 c          565 g         1071 t
ORIGIN      
        1 tatgccttag gtcttttagt ggggaatgat tttttaaaaa atataaaaac cattccaggt
       61 ggtaaaacta atgtagattt aataatcaaa ggatttacta cttcaatgaa aggcgattca
      121 gccattatac ctcttgattc tgccaacaac tatttgagaa attattttac tgcagctcaa
      181 accaaagatt ctgagaaaag aaaaactgca gctattatct ttatggaaca aaacaagaaa
      241 aaagaaggaa taataactac agaaagcggc ttacaatatg aaattttaaa agctactgaa
      301 ggacctaaac ctgctaatac tgacaaagta aaagtcaatt atgaaggttt tttaatagat
      361 ggtactaagt tcgatagctc cattgatcga ggagaaccta tagtttttgc tttaaatcag
      421 gtaattccag gctggagtga aggactttgt ttaatgtcag taggttcaaa atataaattt
      481 tatattcctt acgaacttgc ttatggagca caaggagccg gaaatgtaat acctcctttt
      541 tcacctctaa tttttgaagt agaattgctc gaaattaatc ctgacgaaga aaacaaccaa
      601 taataacatt atcaaaaaat gatgcaatga aaaaattaag tattttgtcc ttaatagtag
      661 taatgactac atttttaatg gtttcgtgta aaaataagta cgaagccaaa aaaagtgaat
      721 tgagcaatca gaatgatagc ttaaattaca cactcggtct ggtgaatgga gaaggtataa
      781 aaaattacta tttacaaaat gattcttccg ataaacctat cgaagcattt atgaacgctc
      841 ttgacaaagc ttacaaaagc aagaaaactg acaaatggta tcaattaggt tatcagattg
      901 gcacttcgct aaaatttcaa gaatctcacg gtttgatggc tgacactact ctggctttca
      961 atataaagct ggtaaagcaa ggaattatca atgctttata caattttgat ggaggaatga
     1021 gccctcaaca agctcaagaa tatcttcaaa aaactatgta cgaattgcag gctaaaaaag
     1081 cttctgaaca agctttacca tctgttccta aaaaagatag cacattagat actcttaata
     1141 cgaaataaaa tttataacaa taaactaatt cgaaatgaaa aagattaata tattctcggt
     1201 tctcatcatt ctaattgctt ttgccattgt ttcctgtaat agtaacaaac cagctaaacc
     1261 agttctgaaa aaccaacttg acagtttaaa ctatgctttt ggtgttgcca atggagatgg
     1321 tattaaaaat tattatttac aaggcgactc ttctgatgaa actataaaat ctcttatcaa
     1381 aggcatgaat gatggactta aatcgaaagt agaaaaagaa catctggaaa tttctttttt
     1441 aggtactcaa attgggtctt cactaaaaga gcaggaaaaa attggcttaa tgggtgactc
     1501 aactttaaaa actgatatca acctaattaa acaaggattg gtgaatggac taaggggatc
     1561 aaacattcaa atggaacctg aagaagcaaa agtatatttg cagagcacca tgcagaatcg
     1621 gcaggctaaa caaattgaaa aagaatatgc tgccactaaa gctgcaggag aattattttt
     1681 aaaagaaaat gccaaaagac cgggagtaat tatcactcca agtggcttac aatacgaaat
     1741 tatcaaaaaa ggaaatggtc ccataccgac tgatagtagt aaagtaaaag ttcattatca
     1801 cggtacgttg atagatggaa ctgtttttga cagttctgtg gagcgtaaag aacctagtgt
     1861 attctatgtc aatcaagtaa tcaaaggttg gcaggaaatt ctaaaattaa tgccagtagg
     1921 ttcgaaatat aaaatttatg ttcctcagga attggcttat ggtggtcagg aacagggatt
     1981 aattaaaccc tattcacctc ttatttttga aattgaatta ctttcaattg aaaaataaaa
     2041 gcagctttat agcaatttca atatcttata aaaggatata aaagaaattt taataaaccc
     2101 tggttgacaa taaactttca acgagggttt tttgtttttt aaaatcttgt ttttctgcat
     2161 tggaacggat taatattttt ttactttgtt tttcttagtt tatttctaat taaaattaat
     2221 tgtcttataa aatcaaaata aatatactat ggacaaaaaa acgagaaaac tattaatatc
     2281 agtacaacga aatgaaatca ctgaacattt tatttataaa cgtttagcag aaggatgtaa
     2341 agatcaaaaa aatgctgaaa ttttacacac cataagcgaa caagaaaaac aacatgcagc
     2401 attttggcaa gaaaaaacca accttaaagt taaaccaaac tattggagaa ttacttacac
     2461 tgtttttctg gctcgtttat tgggactgtc gtttgtatta aaacaaatgg aaaaacgcga
     2521 aggaacaggt tcaaaacttt ataatgaatt gtcaaatatt attcctgaga caaaattttt
     2581 tgctgaagaa gaaagcattc acgaacaaca attattaaca atgctcgatg aagagcgatt
     2641 acaatatgtt ggttctattg tgttagggct taacgatgct ctcgtcgaat taaccggagc
     2701 actggcaggt tttactttaa ctttgggaac aaccagaacc atttcattag caggattagt
     2761 tacaggaata tctgctgcct tgtctatggc ggcttccgat tatctttcct cgaaagcaga
     2821 aggtaataaa aaagccaaaa aatcggctgc ttatacaggt ataacttatt ttttcactgt
     2881 tatttttctc attcttccat ttttattatt atccagcaga ttttttgctt tatcgctaac
     2941 attattactg gcagtattaa taattttctt ttttaattat tatatctctg tggcaaaaga
     3001 gctcgagttt aaatctcgtt tttttgaaat gtgctttatc agcttaggag tagcagcctt
     3061 ttcatttttt ataggttata tacttaatag tatattaggt gtaaatgctt aatagaaaaa
     3121 gttttgagtt tacgaggcgt ggaaagaacg aggatttttt gtagggaaat tttaatttgt
     3181 gtcaaaacaa ccgaagcgaa gctgaaattg cacaaacaat taaaaaatta aacactcttt
     3241 gagacaaaaa agtaaataag gtttgaggaa aagcccctaa tacaaaatat tgttcaataa
     3301 attttagata atttttgtct tgtgtttatg atatttttta taaaattgcg taaaaatttc
     3361 ttccattcaa ttttaaaaaa gcatcaattt tttg
//
LOCUS       DAOTKS010000157         3389 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_23228_length_3389_cov_3.730054, whole genome shotgun sequence.
ACCESSION   DAOTKS010000157 DAOTKS010000000
VERSION     DAOTKS010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23228_length_3389_cov_3.730054"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="P5236_04040"
     CDS             <1..644
                     /locus_tag="P5236_04040"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRU63563.1"
                     /translation="DNGKAFPMMDVDNVNHKALFVTNDGSNIATDKIYKYDLNASSWS
                     EVTTNGLPQNVVMNCLKNIDDNEWLLATNVGLYRSKDGGVNWSIAHSSDYWQNGIIVN
                     SIVKINDKAFLGTMANGVWEVDLSTGVANPLSEKDFLVFPNPAIDKLTIAIPEWNAKS
                     AEITIYNIEGKKVFNTLLTTNPTTVSLNHLTAGTYLVELKSNGTIFHKTVVKK"
     gene            complement(698..3127)
                     /locus_tag="P5236_04045"
     CDS             complement(698..3127)
                     /locus_tag="P5236_04045"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRU63564.1"
                     /translation="MRKTFLLTAFVLFCAMAATGQHFIKGKVLDENQHPLEGANVFIK
                     SEGIGKITNKNGEFFFDHLSRKSYQLDVSYVGFETLKVTTQVDREIIITLKPKDYLMN
                     EIIITSLRANERSAVTYSEVKKEEIEKRNVGQDIPYLLAGTPSFIVTSDAGNGVGYTN
                     FRIRGTDANRTNVTLNGVPLNDAESHGTFFVDFPDLASTISSFQIQRGVGTSTNGAGA
                     FGASINMQTEGTSSKPYADITSSYGSFNTNKNTIKAGTGLINNVFAFDARLSNVTSDG
                     YIDRASVDMKSYYFSAGYYTPKTTIQFLTLGGKEKTYQAWNGVDAEKLKTDRTYNDLG
                     LYYDNEGNIHFYDNQTDNYNQYHYHLHWLQQVNPNLIFNATAHLTRGIGYYEEYKLDR
                     KYPEYGLTPAIIGSDTLKKTDLVRQKWLDNYFYGLIYNLNYQKRNLNFSAGGALNRYD
                     GDHYGKVIWVRNANQLDMSKKWYENTSTKDDANIYAKLNLETFKNLFTSIDVQYRYIN
                     YQMNGRDDKYDEVNKTMRDITQEHTYHFFNPKLGLTWQINRSNDIYGSYSISHREPNR
                     DNFTEAGPNERPTYETLYDTELGYHFKSTLFSAGINLYYMKYKDQLILTGKISEIGEP
                     LTKNIPDSYRSGIELVIGIKPSRWLDWNGNLTLSRNKIRNFVEEDVEEYDADWNYIGV
                     RNNQLGTTDIAYSPNVIMNSIFTFHIHKWDIGFYSNYVGRQFIDNTSDKERSINPYFV
                     NNISLNYSLPLMKIKELQLQLLVNNVFNEKYETNGYTWYSCYLDGKRYNELRYFPQAG
                     TNFLAAVRLKF"
     regulatory      complement(3181..3275)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         1149 a          596 c          531 g         1113 t
ORIGIN      
        1 atgataacgg gaaggctttc cctatgatgg atgtggataa tgtaaatcat aaagctcttt
       61 ttgttaccaa tgatggctcc aatatcgcaa cggataaaat ttataaatat gatctgaatg
      121 cctcttcatg gtcagaagtt acaacaaacg gtctccctca aaatgttgtt atgaattgtt
      181 tgaaaaacat cgacgataat gagtggctct tggcaacaaa tgttggtttg tacagaagta
      241 aagatggcgg ggtaaactgg tcaattgctc attcctcaga ttattggcaa aatggaataa
      301 ttgtaaatag tattgttaaa attaatgata aagctttttt gggcacaatg gcaaatggcg
      361 tatgggaagt tgatttgtcc acaggggtag caaatccttt gtctgaaaaa gattttctcg
      421 tctttccaaa tcctgcaata gataaattaa ctattgcaat tcctgaatgg aatgcaaaat
      481 cagcagaaat taccatttac aacatagaag gtaaaaaagt atttaacacc cttcttacaa
      541 ctaatcctac tactgttagt ttgaatcatt taactgcggg tacttatctt gtagaattaa
      601 aatcgaatgg aacaattttt cataaaactg ttgtcaaaaa ataagctgaa atgaaaaatt
      661 taagagagcc gattcaaacc aatcggctct cttattgcta aaattttaac ctgacagcag
      721 ctaaaaagtt agttccagct tgaggaaaat agcgtagttc attataacgt ttaccatcta
      781 aataacagga ataccaggta taaccattgg tttcgtattt ttcattgaat acattattaa
      841 ccaatagttg cagttgtaac tcctttattt tcattaatgg taaagaataa tttaaactaa
      901 tattgtttac aaaataagga ttaatggagc gttccttatc tgaagtatta tcaataaatt
      961 gtcttcctac ataattggaa taaaatccaa tatcccattt atggatatga aaagtaaaaa
     1021 tactattcat aatcacatta ggagaataag caatatcggt agttccaagc tggttgttac
     1081 gcacaccaat ataattccag tcagcgtcgt attcttccac atcttcttct acaaaattcc
     1141 gaattttgtt tctgcttaaa gtcaaattgc cattccagtc taaccatctg gatggtttta
     1201 tcccaataac aagttcaatt ccagatcgat aactatcagg tatatttttt gtaagtggct
     1261 cgccaatttc gcttattttg ccagtcaata tcaactggtc tttgtatttc atataatata
     1321 aattgatccc tgctgaaaat aaagtggatt taaaatgata ccccaattca gtatcgtaca
     1381 aagtttcata agtaggacgt tcatttggac cagcttcggt aaaattatca cgattaggtt
     1441 cccgatgaga aatagaatag gaaccataaa tatcattact tctattaatt tgccaggtta
     1501 atcctaattt tggattaaaa aaatgataag tatgctcttg agtaatatca cgcatggttt
     1561 tattcacttc atcatattta tcatctctcc cattcatctg ataattgata taccgatact
     1621 gcacatcaat ggaagtaaat aaatttttaa aagtttccag attcaatttt gcataaatat
     1681 tggcatcatc ttttgtggaa gtgttttcat accatttttt gctcatatca agctgattag
     1741 cattgcgtac ccatattact ttaccataat gatcgccatc gtaacgattg agggcaccac
     1801 cagcggaaaa atttaagttt cttttctgat agttcaaatt ataaattaaa ccgtaaaaat
     1861 aattatccag ccatttttga cgaaccaaat ctgttttctt taaagtatct gaaccaataa
     1921 ttgcaggtgt taatccatat tctgggtatt ttctgtcgag tttatattct tcgtaataac
     1981 caattccacg ggttagatga gcagtagcat tgaaaatcaa attgggattc acctgctgca
     2041 accaatgcaa atgataatga tattggttat aattatctgt ttgattatca tagaaatgta
     2101 tatttccttc attatcataa tataaaccca aatcattata ggtacgatca gttttcaatt
     2161 tttcagcatc aacaccattc caagcctgat aggttttttc ttttccaccc agtgtcaaaa
     2221 actgtattgt tgtttttgga gtataatagc cagcggaaaa ataatacgat ttcatatcta
     2281 ccgaagcacg atcaatataa ccatccgaag taacattcga taatcgagca tcaaaagcaa
     2341 atacgttgtt aatcaatcca gtacccgctt taattgtgtt tttgtttgta ttaaatgaac
     2401 catacgaaga agttatatca gcatacggtt tagatgaggt tccttcggtc tgcatattga
     2461 tactggcacc aaatgcaccc gcaccattgg ttgaggtccc aacaccgcgt tgtatttgaa
     2521 aagaagaaat cgtcgaagcc aaatcaggaa aatcaacaaa aaaagttcca tgtgattctg
     2581 catcattcaa cggaactcca ttaagggtta catttgtacg attagcatca gtgccacgga
     2641 ttctaaaatt ggtataacca acgccattac ccgcatcgga agtaacaata aaagaaggtg
     2701 ttccagccaa taagtaagga atatcttgac caacattgcg tttttcaatt tcttcttttt
     2761 ttacttcaga ataagtaaca gccgaacgct cattggcacg taaagaagta ataataattt
     2821 cattcattaa ataatcctta ggttttaaag ttattattat ttcacggtca acctgagttg
     2881 ttactttcaa tgtttcaaaa ccaacgtaag aaacatccaa ctggtaactt tttctggaaa
     2941 gatgatcgaa aaagaattct ccatttttgt tagtgatttt cccaatccct tcgcttttaa
     3001 taaaaacatt cgctccttca aggggatgtt gattttcatc aagcacttta cctttaataa
     3061 agtgttgtcc agttgctgcc atagcacaaa acagcacaaa ggctgtcaac aaaaaagttt
     3121 ttctcataac aaattataat taaatgatta ataaaatgtt ttgagagcct aataaaaacg
     3181 cagatgattt ttctttttcc cttcacggaa ttacccgtgc aggttcaaag ggtatgatct
     3241 cagcccgaaa tattaaggca ccccatttat aaaatgaaag taatggaata aaaaccgcgg
     3301 attttcattt cattttcacc acaaaattac ataaatcttt ttgtaggaaa aaatttccgg
     3361 aaaaattgtt taacacaatt tgtcattaa
//
LOCUS       DAOTKS010000158         3388 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_23242_length_3388_cov_2.910891, whole genome shotgun sequence.
ACCESSION   DAOTKS010000158 DAOTKS010000000
VERSION     DAOTKS010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23242_length_3388_cov_2.910891"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3224)
                     /locus_tag="P5236_04050"
     CDS             complement(<1..3224)
                     /locus_tag="P5236_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRU63565.1"
                     /translation="MALNTKAQDAVIELNNNSLTLGQLINEIERQTDYLVVYSNREID
                     TNQEINLPNKSDKVSTYLNEAFKRTDIAYEFENNYIVLLKRSNQNASTVANLIQAAQQ
                     SQQKEISGKITDSSGDPIPGATVMVKGTTIGTITDADGKFFLRIPANAQTLQISFIGM
                     KNQEIPIGERTTFNIQLEEETVALEEVVAIGYGVQKKVNLTGAVASVRSDDILKAQSA
                     NTANTLVGQIPGLIAKQPTGEPGNDNSQLFIRGVATFRGSTAPTFIIDGIERTYEDFA
                     RLDPHEIESLSVLKDAASAAVFGMRGANGVILVTTKRGRPQKIQASYSGNISTQSPTK
                     LPSFANSADYARMKNIYMNKEIYTPEEIEKFANGSDPENYPNTNWYKETLTKNAIQQQ
                     HNITLNGGREDIAYFTSFGYLNQGGLWNNLNYDRYSLRANIDANITKSTKLSVDISGR
                     VEHTHSSPTNSTGVFQQLVRNTPILLAKNKDGLLIVPDATHPNIIALISKEAGYNNSR
                     NNTVTPLIELEQNLSFITDGLKIKGVFSYEKNNYAQKLWSKSPSLYVRDPLNKDNYIP
                     QPHGSPNLYENTYDNEYIEWQGHVSYNKSFKNHSFSVLAMGLGQKENYHNLWVQRNSF
                     DSEVMDQINAGNTQGQQLGGYDEEHARVSFVGRINYSYANKYLFEVNSRRDASENFSP
                     EKRWGTFSSVSGGWVMSEENFFEDLRKEINFLKFRVSYGTLGNDNTGGIAFPYYSRFD
                     LYFPNGAHAGGLPNDLGDYIFGNLVTKGLQPGPIANPLAAWETSKKTNVGMDWGLFNI
                     INLSLDVFKENRFDILAQRGSEVPWSFGGILPLENIGKVVNKGIELLLSFNQKIGQFH
                     YNLGGNFTYARNKVLEMAEAAGTTKYMKHTGRPIYSYYGYKTDGIFQSQAEIDAYAKQ
                     EVAGIDYVTKPGDIKYVDVDGNGVVNAGDMTYLGYGNVPEIVYGINGSISYKNFDFSF
                     LFQGTGHVQVYLNGGVIMPYYNDGNLPSLWIKNAWTEEKPNSRYPRLEASNHNFPNTE
                     FPPVQTYLYDASYLRLKNIEIGYNFNYKWLVNNHIENIRIYLSG"
BASE COUNT         1076 a          613 c          500 g         1199 t
ORIGIN      
        1 tgtccactca aataaatacg gatattttct atatgattgt taacaagcca tttataattg
       61 aagttataac caatttcaat attttttaag cgtagataag aagcgtcata taaatatgtt
      121 tgaactggcg gaaattcggt attcggaaaa ttatgatttg aagcttctaa tctggggtaa
      181 cgagaatttg gcttttcttc agtccaagca ttctttatcc ataaggaagg taaatttcca
      241 tcattataat aaggcataat tacgccacca tttaaatata cttgaacatg acctgtacct
      301 tgaaataaaa aactgaaatc aaaattttta taggaaatag agccatttat gccgtaaaca
      361 atttcaggaa cattaccata accgagataa gtcatgtcac ctgcatttac aaccccatta
      421 ccatctacat ctacatactt aatatcacct ggtttagtta cataatctat tcctgctact
      481 tcttgtttag catatgcatc tatttctgct tgtgactgaa aaattccatc agttttataa
      541 ccataataac tataaattgg tctccctgta tgtttcatat atttagttgt gccagctgct
      601 tctgccattt ccaatacttt atttcgcgca taagtaaaat ttcctcctaa gttatagtgg
      661 aattgaccaa ttttttgatt aaaagaaagc aataactcta tccctttgtt tactactttt
      721 ccaatattct ctaaaggcaa aattccacca aatgaccatg gaacttcact accgcgttga
      781 gcaagaatat caaaccgatt ttctttgaat acatcaagag acagattaat tatattaaaa
      841 agtccccaat ccatcccaac attagttttc tttgaagttt cccaagctgc taaaggatta
      901 gctattggtc ctggttgaag ccccttggta actaggttac caaaaatata atcacctaag
      961 tcattgggta aaccaccggc atgagcccca tttggaaaat acaaatcaaa gcgactataa
     1021 taaggaaaag caatgccgcc agtattatca ttacctaatg ttccatatga tactctaaat
     1081 ttaaggaaat ttatttcttt tcttaagtct tcaaaaaaat tttcttcaga cattacccaa
     1141 cctccagaaa cagaactaaa agttccccat cgtttttcag ggctaaaatt ttctgaagca
     1201 tctcttcgtg aattaacttc aaaaagatat ttattagcat aagaataatt tattcgtcct
     1261 acaaaactta ctcgtgcatg ttcttcgtca taaccaccta attgttgccc ttgtgtattt
     1321 ccagcgttaa tttgatccat tacttcagaa tcgaatgaat tacgttgaac ccataaatta
     1381 tgataatttt ctttttgccc tagccccatc gctaagactg aaaaagagtg atttttaaaa
     1441 gatttattat aactcacatg tccttgccat tcaatatatt cattatcata agtattttca
     1501 tataagttag gactaccgtg tggttgtgga atataattat ctttatttaa aggatctcgt
     1561 acatataagg atggcgattt agaccataat ttttgagcat aattattttt ttcataagaa
     1621 aaaactcctt ttatttttaa accatcagta ataaaagaaa gattttgttc caattcaata
     1681 agaggagtaa cagtattgtt tcttgaatta ttatatccag cctctttact aattaacgct
     1741 attatattcg gatgagttgc atcaggcaca ataagtaaac catccttatt ttttgctaac
     1801 aatattggcg tgttacgtac aagttgttga aaaacaccag tgctgtttgt tggactacta
     1861 tgagtatgct caactcttcc agaaatatct acagataatt tggtggattt agttatatta
     1921 gcatcaatat ttgctcttag tgaatagcga tcataattta aattattcca aagtccgcct
     1981 tggtttagat agccaaaact agtaaagtag gcaatatctt cacgtccacc atttaaagta
     2041 atattatgct gttgctggat tgcatttttc gtaagagttt ccttatacca atttgtatta
     2101 ggataatttt ctgggtcgga gccatttgca aatttttcaa tttcttcggg agtataaatt
     2161 tctttattca tgtatatatt cttcattctc gcataatctg cagaattggc aaagctagga
     2221 agcttagtcg gactttgagt agaaatattc cctgaatagg aagcttgaat tttttgtggt
     2281 cttccacgtt ttgtcgtcac tagaatcact ccattagcac cacgcattcc aaaaacagct
     2341 gcagatgcag catcttttaa aacacttaat gattcaattt catgtggatc taaacgagca
     2401 aaatcttcgt atgttctttc tatgccatca ataatgaatg ttggtgcggt acttcctcta
     2461 aaagtggcaa ctccacgaat aaaaagttgt gaattatcat ttcctggttc tccagtaggt
     2521 tgttttgcaa tcaagcccgg tatttgtcca actaaagtat tagccgtatt agctgattgt
     2581 gcttttagaa tatcatcact tctcactgaa gcaacagctc cagtcaaatt tactttcttt
     2641 tgtactccat aaccaattgc taccacttct tccaaagcaa ccgtttcttc ttccaactga
     2701 atattaaacg tagttctctc tccaatgggt atctcctggt ttttcattcc aatgaaagaa
     2761 atctggagtg tttgagcatt agcaggaata cgaaggaaaa attttccatc tgcatctgta
     2821 attgtcccaa tagtagtacc ttttaccatt acggtagcac ccggaatagg atcacccgac
     2881 gaatctgtta tttttcccga aatttctttt tgctgtgatt gttgagctgc ttgaataagg
     2941 ttggcaactg ttgaagcatt ttgattggat cttttcaata atactatata gttgttttca
     3001 aactcataag ctatatctgt tcttttaaaa gcttcattca aataagtgga tactttgtct
     3061 gatttatttg gaagatttat ttcttgattt gtgtctattt cgcgattgct ataaactacc
     3121 aaataatcag tttgccgttc aatttcattg atcagttgac ctaaggttaa tgaattattg
     3181 tttaattcaa taaccgcatc ttgggcttta gtatttaaag ccatcaactg aaaagagaaa
     3241 acaaataaaa gaataaaaca gattctcata attctgaata tctctttaaa agtttcattt
     3301 tttattaaat aaagtacttt tagaaatttt tttttcatat ctttgaaaaa ttaaaagtga
     3361 atactaaaaa aagcttgtag gtgtttac
//
LOCUS       DAOTKS010000159         3385 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_23267_length_3385_cov_3.608108, whole genome shotgun sequence.
ACCESSION   DAOTKS010000159 DAOTKS010000000
VERSION     DAOTKS010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23267_length_3385_cov_3.608108"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="P5236_04055"
     CDS             complement(<1..300)
                     /locus_tag="P5236_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HRU63566.1"
                     /translation="MKSKLNNYFIATRPWSFPASTMPALITLTYIFYINEEISGNINW
                     WYGIIAIIGAAIFQASSNLINDYFDYKYQVDRPESFGSSRLLVEKVFTPKFILSYG"
     gene            complement(333..2225)
                     /gene="mutA"
                     /locus_tag="P5236_04060"
     CDS             complement(333..2225)
                     /gene="mutA"
                     /locus_tag="P5236_04060"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase small subunit"
                     /protein_id="HRU63567.1"
                     /translation="MSEKKLNLLADFSPVSTEEWMKKIMEDLKGADFEKKLVWKTDEG
                     FNVQPFYRLENIENLKTTELLPGKFPYVRGTKTDNEWFIRQDIKVENIKEANAKALDI
                     LNKGINSLGFDVRGYKISSSLLKDLLENINAECIELNFLVDANNAITLANTLSEYLLS
                     QQYDVKNLQGAIEFDPIGEMLQDGKKKSKEQIVEQLKSVIDAISKLPFYRVMTVNATI
                     LKNAGAFITQELGNALAWGNQYLSMMVDAGVDTSLAAKKIKFNFGIGSNYFMEIAKFR
                     AARLLWALIVDAYQPVCSCEDNSNAKDGTCKCATKMRIHAETTNFNKTIFDAHVNMLR
                     TQTEAMSAVLGGIDSLTVLGYDDVFNAPNEFAERIARNQQLLLKEESHFDKVVDPAAG
                     SYYLEILTKEIAQQAWKLFLEIENEGGFLEAIYAGKVQQAIKATANYRMKNISNRKEI
                     LVGTNQYPNFNETASEKLVNCPQCDKNNQSTEVETLPPVRGAAEFETLRLATERAAKR
                     PKVFMLTIGNLAMRVARSQFSCNFFACAGYEVIDNVGFDTVEEGVQAALEAKADIVVL
                     CSSDEEYAQFAPAAYQLVKDKAIFVVAGAPACMDDLKTQGIENFISVKSNVLEILKSF
                     NEKLGI"
     gene            2519..>3385
                     /locus_tag="P5236_04065"
     CDS             2519..>3385
                     /locus_tag="P5236_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HRU63568.1"
                     /translation="MKKIAFFILAFSCIFSLSAKRVMTGIEVLKEQNFKLLEGKRVGL
                     ITNPTGVDVNLKSTIDLLNEAPNVKLVALYGPEHGVRGNVYAGGTISNEIDEKTGLPV
                     YSLYGKTRKPTPEMLKGIDMLVYDIQDVGCRSYTYISTLGLAMEAAAENNIEFVVLDR
                     PNPLGGEKIEGNITEDAFISFVSQFKIPYIYGQTCGELAFMLNEENMLKKKCKLTIVK
                     MKNWNREMKWEDTGLEWVIASPHIPHKHSSYFYPVTGILGELNYLSIGVGYTLPFEIV
                     AAPWIKADELAKA"
BASE COUNT         1074 a          596 c          576 g         1139 t
ORIGIN      
        1 accataagaa agaataaatt tcggcgtaaa aactttttca accaataacc ggctagaacc
       61 gaaagattca ggacgatcta cctgatattt ataatcaaaa taatcattga ttaaattact
      121 gctggcttga aaaatggctg ctccaataat tgctataata ccataccacc agttgatatt
      181 tccactgatt tcttcattga tataaaaaat ataagtgaga gttattaatg ctggcatggt
      241 agaagcaggg aaagaccacg gccgcgtagc aataaaataa ttgttgagtt ttgacttcat
      301 ttacatcatt tcatttttga aaaattcttt cattaaatac ccaacttttc attgaaactt
      361 tttaaaattt ccagaacatt ggattttaca ctaatgaaat tttctatacc ttgtgttttt
      421 aaatcatcca tgcaggctgg agcacccgct accacaaaaa tcgctttgtc ttttactaat
      481 tgataagctg ctggggcaaa ttgtgcatat tcttcatcgc ttgaacatag aaccacaata
      541 tccgctttag cttccaatgc agcctgtaca ccttcttcca ctgtatcaaa tccgacattg
      601 tctatcactt cataaccagc acaggcaaag aaattgcagg aaaattgtga acgagctact
      661 cgcatagcca gatttccaat ggttagcata aaaacttttg gcctttttgc tgcacgctcg
      721 gtagcaagac gtaacgtttc aaattctgct gcacctcgaa caggtggtaa ggtttctact
      781 tcagtagatt gattgttttt atcacattgc gggcaattaa ccagtttttc tgatgctgtt
      841 tcattaaagt taggatactg attggttcca accaatattt cttttcgatt ggaaatattt
      901 ttcattcggt aattggcggt agctttgatg gcttgttgaa ctttgccagc ataaatagct
      961 tctaaaaagc ctccttcgtt ttcaatctcg aggaacaatt tccaggcttg ttgagcaatt
     1021 tctttggtga ggatttcaag atagtaagaa cctgctgctg gatcaaccac tttatcaaaa
     1081 tgagattctt ctttgagtaa tagttgttga tttctggcga tacgttcagc aaattcgttt
     1141 ggagcattaa atacatcatc ataacccaga acagtcaatg aatcaattcc accgagcaca
     1201 gcactcatgg cttctgtttg ggtgcgaagc atatttacat gagcatcaaa tattgtttta
     1261 ttgaaattag tcgtttctgc atgaattctc attttagtag cacatttaca agtaccgtcc
     1321 tttgcatttg aattatcttc acaagaacat acaggttgat aagcatccac aataagtgcc
     1381 cacagaagtc gagcagctct gaattttgca atttccataa aataattgct gcctattccg
     1441 aaattaaatt taattttttt agcagccagc gaagtatcaa ctccagcatc caccatcatg
     1501 gaaagatatt gatttcccca ggccagagca ttgcctaatt cctgtgtaat aaaagctccg
     1561 gcatttttca aaattgtagc atttaccgtc attacacgat aaaaaggtaa ttttgaaatg
     1621 gcatcaatga ctgattttaa ctgttcaaca atttgttcct tgcttttctt tttgccatct
     1681 tgcaacattt caccaatagg atcaaattca atagcaccct gtagattctt tacgtcatat
     1741 tgctggctca gcaaatattc agatagagta ttggctaatg ttatggcatt gtttgcatcg
     1801 acaagaaaat ttagttcgat gcattcagca ttaatatttt ccaataaatc ttttagcaat
     1861 gaagaagaaa ttttgtatcc tcttacatca aaacctaagg aattgattcc tttgttcaaa
     1921 atatccaatg ctttggcatt tgcctctttt atattttcaa ctttaatgtc ctgacgaata
     1981 aaccattcgt tatccgtttt tgtaccccga acataaggaa atttacccgg caataattct
     2041 gtagttttta agttttcaat gttttctaat cggtaaaatg gttgaacatt gaaaccttca
     2101 tcagttttcc agacaagttt tttctcaaaa tcagcacctt tcaggtcttc catgattttt
     2161 ttcatccatt cttcagtgga aacaggtgaa aaatcagcca ataagtttaa ttttttttct
     2221 gacatatttt ttatttttca aaaattatta tcgactgcaa aattactact ttaagtttag
     2281 ttattaaatg tttttggaga gaaaaactaa aaaacgtttt ataaaattaa gttggtaatc
     2341 acttttttaa aaaataattt tttagaaggt tttttcttga tgattaaaat tcaatttttt
     2401 tatttacatt tgcttcaaaa tgcaaacgtt tgcaccaatg ttttatttaa aaattccttt
     2461 aagaggaaag agagttaata taaatctata aatttattca aaaagatcgt atcatcaaat
     2521 gaaaaaaatt gcattcttta ttctggcatt ttcttgtatt ttcagcttgt cagccaaacg
     2581 tgtaatgaca ggaattgagg tattaaaaga acaaaatttt aaattactgg aagggaaacg
     2641 agtaggttta attaccaatc ctacgggagt ggatgtcaat ttaaaatcaa ccattgacct
     2701 tctcaacgaa gctccaaatg taaaattagt tgctttgtat ggtccagaac atggtgttcg
     2761 gggaaatgta tatgcaggcg gaacaatcag caatgaaata gatgaaaaaa cgggccttcc
     2821 tgtttattcc ttgtatggaa aaacccgcaa accaactcca gaaatgttaa aaggaattga
     2881 tatgctggtt tatgatattc aagacgtcgg ttgtcgctct tatacttata ttagcacatt
     2941 gggactggct atggaagctg ctgctgaaaa taatattgaa tttgtggtgc tggatcgtcc
     3001 caatccgttg gggggagaaa aaattgaagg aaatattaca gaagacgcct tcatttcctt
     3061 tgtgagccaa tttaaaattc cgtacattta tgggcaaact tgtggagagt tggctttcat
     3121 gctcaacgaa gaaaatatgt tgaaaaagaa atgtaaactt actattgtaa aaatgaaaaa
     3181 ttggaatcgt gaaatgaaat gggaagatac aggattagaa tgggtcattg cttctcctca
     3241 cattccacat aagcactctt cttattttta tccagtgact ggaattctag gagaattgaa
     3301 ttatctttca ataggggtgg gttatacttt gcctttcgaa atagtagcgg ctccgtggat
     3361 caaggcagat gaattagcta aggct
//
LOCUS       DAOTKS010000160         3375 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_23363_length_3375_cov_4.375301, whole genome shotgun sequence.
ACCESSION   DAOTKS010000160 DAOTKS010000000
VERSION     DAOTKS010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23363_length_3375_cov_4.375301"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2097
                     /gene="porU"
                     /locus_tag="P5236_04070"
     CDS             <1..2097
                     /gene="porU"
                     /locus_tag="P5236_04070"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HRU63569.1"
                     /translation="ATTEEVYNEFSSGSPDATAYRWVMKMFYDRALNANRPDDLPKYL
                     LLFGRGSYDNRGIITNSGDNLILTYQADNSLVITSSYVTDDYFGFLDDNEGIDLTSNL
                     LDIGVGRFPVSTDQQATDIVNKTVNYIQNKGKGIWKNQLCFLADDGDYALHMTQADSI
                     ATSIARLYPGYQINKIYLDAFVQEVNASGQSYPVAKSRFQNLLRSGMFLLDFMGHANS
                     GGWTNEAILTTADIKNLTNQHLAVWMAATCDFLQFDIKPISAGEHVVLNPSGGGIGLL
                     SAARPVYASQNFYINKYFVESLFKKENENHLRVGDAIRMAKNKIGAEINKLCYVYVGD
                     PAVMLNYPDKFSVVTSKINGNTQLGNDTLKALSVTTIEGYISDNVGNKIPDFNGKIEV
                     VVYDKLQKITTLNNEKDGALTYYDRPNTLFAGKTDVKNGEFSFTFMLPKDIKYNYGTG
                     RINYYAYDDDHDYEAQGYFENFLVGGSSQSYVNDTIGPKLTIYLNSPEFVSGGKVNET
                     PLFVAHLEDEHGINAVGSGIGHDLVLIIDANPLTSYILNDYYQSEENSYTSGSVSYKL
                     PELKEGKHTLTFRAWDLLNNSSTQTLDFEVVKGLKPVIFSVYTYPNPVQQRTKIVINH
                     DRPETQLITTVEIFDITGRKIWSFSQSSIDDITWDLKGADDKRVQNGIYLYRVSIKTP
                     NSEMTSKTNKIIVVGQ"
     gene            2170..3297
                     /gene="porV"
                     /locus_tag="P5236_04075"
     CDS             2170..3297
                     /gene="porV"
                     /locus_tag="P5236_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HRU63570.1"
                     /translation="MKKRCSILSVCFFLAFYGFSQKNNPVITAVPSLTIAPDARAGAM
                     GDVGAATQVDVNSQYWNPAKYAFMESSAGVAFSYTPWLSKLVSDINLYYLAGFWKFDP
                     LQSVSASLRYFSLGDIDIMDEFGYPQGAAHPNEFAFDIAYSRLLSTKFSGSVAFRYIR
                     SDLNSGVNLSGGTEMYPGNAFAADIAGYFKTPIYMSSGDGNLAFGFNISNIGSKISYD
                     EKETSYFIPTNLRLGGSFEYPIDDYNKISFNADINKLLVPTQDTMSIAEYSDISSISG
                     IFRSFSDAPGGFSEELQEVMWSVGAEYVYNKQFSVRAGYFHESQNKGNRKYFTVGAGF
                     KLNVFELDAGYVIATNQSNPLDQTLRFTLAFDINGLKNLIK"
BASE COUNT         1077 a          507 c          628 g         1163 t
ORIGIN      
        1 gctactaccg aagaggttta caatgaattt tcttccggat cgccagatgc tactgcttat
       61 cgctgggtaa tgaaaatgtt ttacgacaga gctttgaatg ccaatcgacc tgatgattta
      121 ccaaaatatt tgttgctttt tggacgtggc tcttacgata atcgaggaat tattactaac
      181 tcaggcgata atctgatatt gacttatcaa gctgataatt cgcttgttat tacttcttct
      241 tatgttacag atgattattt tggtttctta gatgataatg aaggtataga cttgacttca
      301 aatttattag atattggcgt gggacgtttc cccgtttcaa cagaccaaca agctactgat
      361 atcgtaaata aaacggttaa ttatattcaa aataaaggaa agggaatctg gaaaaatcaa
      421 ctttgttttc ttgctgatga tggagattat gctttgcaca tgacgcaggc tgatagcata
      481 gccacttcca tagcacgatt gtatcctggt tatcagatta ataaaattta tttagatgct
      541 tttgtacagg aagtgaatgc aagcgggcaa tcttatcccg tagcaaaaag tcgtttccag
      601 aatttattac gttcaggtat gtttttgctt gattttatgg gtcatgctaa ttctggtggc
      661 tggacaaatg aagcgatact tacaactgct gatataaaaa atttaactaa tcaacatctg
      721 gctgtatgga tggcagctac atgcgatttt cttcaatttg atatcaaacc aatttctgct
      781 ggtgaacacg ttgttttaaa tccttcagga ggtggtattg gcctcctttc ggccgctcga
      841 cctgtatatg catctcaaaa tttttatatt aataagtatt ttgttgaaag tttatttaaa
      901 aaggaaaatg aaaatcacct gcgcgtagga gatgctataa gaatggctaa aaacaaaatt
      961 ggtgctgaaa ttaataaact ttgttacgta tatgtaggtg atcctgctgt aatgttaaat
     1021 tatccagata aattttcagt agtaactagt aaaataaatg gaaataccca gttaggaaac
     1081 gatacattga aagctttgtc tgtaacaaca attgaaggct atattagtga taatgttgga
     1141 aataaaatac ctgattttaa tggaaaaatt gaagtagttg tttatgataa attacaaaaa
     1201 atcactactt tgaacaatga aaaagatgga gctcttactt attatgatcg tccaaacact
     1261 ttgtttgctg gaaaaacaga tgtgaaaaat ggagaattct cctttacttt catgcttccc
     1321 aaagacatca aatataatta tggaaccgga agaattaatt attatgctta tgatgatgac
     1381 catgattatg aagcacaggg ctattttgaa aatttccttg taggaggcag ttcacaaagt
     1441 tatgtaaatg atacaattgg tcccaaatta acaatttatt tgaattcccc cgaatttgtt
     1501 tcaggcggaa aagtaaatga aactccttta tttgtagctc atctggaaga tgaacatggc
     1561 atcaatgcag taggaagtgg cattgggcat gatttggtat taattattga tgcaaatcct
     1621 ctaacttctt atattttgaa tgattattat caatcagaag aaaatagtta tacgagtgga
     1681 agcgtttctt ataaacttcc tgaactgaaa gaaggaaaac atactttaac tttcagagcg
     1741 tgggatttat taaataattc ttctacgcaa acgcttgact ttgaagtagt aaaaggctta
     1801 aaacctgtga ttttctcggt ttacacttat ccaaatcctg ttcagcaaag aacaaagatc
     1861 gttatcaatc atgatcgacc agaaacgcaa ttaattacaa cggtagaaat ttttgatatt
     1921 actggacgaa aaatttggtc attctctcaa tcttctattg acgatattac ctgggactta
     1981 aaaggtgccg atgacaaacg agtacaaaat ggtatttatt tatatcgtgt tagcattaaa
     2041 acacccaata gcgaaatgac ttcgaagaca aataaaatca ttgttgttgg acaataaaaa
     2101 ttaaaaatca cgtttgtata aaatacattt tttaaaataa tctattagta actaaataaa
     2161 taagaacgaa tgaaaaaacg atgttcaatt ttgagtgttt gtttcttttt agctttttat
     2221 ggtttttctc agaaaaataa tcctgttatt acagcagtac cttcattaac tattgctcct
     2281 gatgctcgag ctggtgcaat gggtgacgta ggagctgcta cgcaagtcga tgtaaattcc
     2341 cagtattgga atccagccaa gtatgctttt atggaaagta gtgcaggagt tgctttctca
     2401 tatactccct ggcttagcaa attagtcagt gatattaatt tatactattt agctggtttt
     2461 tggaaattcg atcctcttca atcggtaagt gcatcccttc gttatttttc tttaggtgat
     2521 attgatataa tggatgaatt tggttatcct caaggtgctg ctcatcctaa tgagtttgca
     2581 ttcgatattg cctattcacg tttgctttca actaaatttt caggttcagt ggcttttcgt
     2641 tatattcggt cagatttgaa tagtggagta aatttgagtg gaggtacaga aatgtatcca
     2701 ggcaatgctt ttgctgcgga tattgctggt tattttaaaa cacctatata tatgtcaagc
     2761 ggagacggga atttagcttt tggttttaat atttctaata ttggatctaa aatctcttat
     2821 gatgaaaagg aaaccagtta ttttattcct accaaccttc gtttgggagg ctcatttgaa
     2881 tatccgattg atgactataa taaaattagt tttaatgcgg atataaataa attacttgtt
     2941 cctacacaag acaccatgtc tatagctgag tatagtgata tttcatcaat cagtggtatt
     3001 ttcaggtcat ttagtgatgc accaggtggt ttttctgaag aattgcaaga agtaatgtgg
     3061 tcagtgggtg ctgaatatgt atataacaag caattttcgg ttcgtgcagg ttattttcat
     3121 gaaagtcaaa ataaaggcaa tcggaaatat tttaccgtag gagctggttt taaattaaat
     3181 gtatttgaac ttgatgcagg ttatgttatt gctaccaatc aatccaatcc tttggatcaa
     3241 acactgcgat ttacgttagc atttgacata aatggcttga aaaatttaat aaaataaatt
     3301 tagatttttg tatttatctt tgcggttgtt cccatattgt tcaattctgt ggttgaacaa
     3361 ccgttttttt tttat
//
LOCUS       DAOTKS010000161         3356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_23546_length_3356_cov_3.161466, whole genome shotgun sequence.
ACCESSION   DAOTKS010000161 DAOTKS010000000
VERSION     DAOTKS010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23546_length_3356_cov_3.161466"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="P5236_04080"
     CDS             complement(<1..634)
                     /locus_tag="P5236_04080"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRU63571.1"
                     /translation="MNKKSNLCALILLFAFNSSFYGQITSDTISIQLSDVEVVRQRNK
                     LYSELGRVLTVINKEEINRMPVHSIDELLDQISGLDIRQRGTFGVQADISVHGGSFDQ
                     VLVLLNGVNITDPQTGHFNLDIPLNFNEIDRIEILQGSAARLLGPNAFSGAINIITNQ
                     NKKQNQLKAELSAGSNEYSTQNISTDFNIKNVTNFISFSHKSSDGYIENTD"
     gene            complement(627..1034)
                     /locus_tag="P5236_04085"
     CDS             complement(627..1034)
                     /locus_tag="P5236_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63572.1"
                     /translation="MKIHHTFSFKKNVRFRRWCKKGYAVFCSLTHQVTIGVLAASICD
                     KSTLKSKKNILTDIPSEKNEFVSFIEKGPLINEDFLLLFNNSITINFSIVSSNDVILP
                     LANQLINFTQRLRKVPSFFNRFFINFKKNYPNE"
     gene            complement(1089..2471)
                     /locus_tag="P5236_04090"
     CDS             complement(1089..2471)
                     /locus_tag="P5236_04090"
                     /inference="COORDINATES: protein motif:HMM:NF039698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6242 domain-containing protein"
                     /protein_id="HRU63573.1"
                     /translation="MPSPYLKLSIFCVAIIFLNSCLGDDYVTDTSNNPCFVSLSFLKN
                     DSVPNLENAVFTLVFDETIHDSIIVNLDSLPYQTRIDSVYAVFSFKSSSACYLIFDND
                     SLVTYSSGNPIDFTKVSQIKNIASDGVTERTYYIKVNVHQVDPELYVWKKLIDNVYSF
                     TAQDQKAIYFKGKIYFFVSSNAGNFLYNSTNGTSWTEYTINGLPPLATFQDIQIFNDT
                     TLFLTQNNSNLYFSTDGINWSLYQYADFQFSSLLFVMNNQLWAIVNTINDGKYRSASS
                     NDGSYWQLNNEIPANFPISNFTSISFYSRTKTPKALVINGTSVDNQPLGTRWSTENGS
                     YWVDFSIEDKSLDSLDQGAPVIFYDDKLFILGRNKEKTEKNYFRQSIDEGLSWQIPDS
                     NYNYLPEDFQARHYSSFFVFNPRPYDKTDKKEQIEASNRIYIIGGKNSSGWFSDVWTG
                     KVNKKNFLIQ"
     gene            complement(2574..3335)
                     /locus_tag="P5236_04095"
     CDS             complement(2574..3335)
                     /locus_tag="P5236_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008765510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRU63574.1"
                     /translation="MKIISYNINGLRAALTKNFTDWLQAENPDVLCLQEIKAQPDQID
                     TWLFAELGYITYIHSAEKKGYSGVATLTKIPPDNVVMGMQNPVYDAEGRVLRTDFEDI
                     SILNVYIPSGSSGGERQQFKMQFLEDFLPFIKELLKLRPNLVVCGDFNICHKPIDINH
                     PELQEGVSGFLPEEREWMDRFQEAGFVDSFRVFDQSPGKYTWWSFRGGARSRNAGWRI
                     DYHWISKPLLPRLKSAKILSEVFHSDHCPVMIELE"
BASE COUNT         1163 a          542 c          567 g         1084 t
ORIGIN      
        1 aatcggtatt ttctatataa ccatcactac ttttgtggga aaaagaaata aaattagtaa
       61 catttttaat attaaaatca gtagaaatat tttgagttga atattcattg ctaccagccg
      121 ataattcagc tttgagttga ttttgctttt tgttttggtt ggttataatg ttgatagccc
      181 cactaaaagc attcggtccc agtagtcgtg ctgcagagcc ttgtaaaatt tctattctat
      241 ctatttcatt gaaatttagt ggaatatcca gattaaaatg tcctgtttgt gggtcggtaa
      301 tgttaactcc atttaaaagc actaaaactt gatcaaatga gccgccatga acagaaatat
      361 cagcttgtac gccaaaagtc ccgcgttgac gaatatccaa gccagaaatc tgatcaagaa
      421 gttcatcgat actgtgtaca ggcattcgat tgatttcttc tttgttaata acggtaagca
      481 ctcgtcccag ctcggaatat aatttgttgc gttgcctaac tacttccacg tcagaaagtt
      541 gaatacttat ggtatcgctg gtaatttgtc cataaaaaga agagttaaaa gcaaataaga
      601 gtataagagc acataaattt gattttttat tcattgggat aatttttttt aaaattaata
      661 aaaaaacggt tgaaaaaaga tggaactttc ctcaaccgtt gggtaaaatt aattaattga
      721 tttgccaatg gcaaaataac gtcattagaa gatactatcg aaaagttaat agtaatagaa
      781 ttattgaata gaagtaaaaa atcttcattt attaaaggtc ctttttcaat aaagctgaca
      841 aattcatttt tttctgacgg aatatcagtc aaaatatttt ttttagattt taaagtggat
      901 ttgtcgcaaa tagaagcagc aagaactcca atggtaacct gatgggtgag gctacaaaaa
      961 acggcataac cctttttgca ccagcggcga aaacgaacat tttttttaaa tgaaaaagtg
     1021 tggtgtattt tcattcttcg gcaaaaaggg atagatcttt aaaattcatt gaacttttac
     1081 agattcagtt attgaataag aaagttcttt ttatttactt ttcctgtcca cacatcagaa
     1141 aaccaaccgg atgaattttt tccaccaata atgtaaattc ggttagaggc ttcgatttgt
     1201 tcctttttat ctgttttatc ataaggacgt ggattgaata caaaaaatga agaataatgg
     1261 cgagcttgga aatcttcggg taagtaatta taattactat caggaatttg ccagcttaat
     1321 ccttcatcaa tagattgacg aaaataattt ttttcagttt tttctttgtt tctacccagt
     1381 atgaacaact tgtcatcata aaagataacg ggagctcctt gatcgagaga atccaacgat
     1441 ttgtcttcta tactaaaatc aacccaataa gaaccatttt cagtactcca tctggttccc
     1501 aatggttgat tatcaaccga tgttccattt attaccagtg cttttggcgt tttagttcgc
     1561 gaataaaagg agatggatgt aaaattgctg atggggaagt ttgcagggat ttcgttattt
     1621 aactgccaat aacttccatc attggacgaa gcagagcgat atttgccatc attaatggta
     1681 ttaacaatag cccataactg gttattcatt acaaaaagta aagaagagaa ttgaaaatcg
     1741 gcatattgat atagggacca atttattcca tctgttgaaa aataaagatt ggaattattt
     1801 tgtgtcagga aaagtgttgt atcattaaaa atctggatat cttgaaaagt agccaaagga
     1861 ggcaaaccat tgatggtata ctcagtccat gaagtgccat tggtagaatt atacaaaaaa
     1921 tttccagcat ttgaactgac aaagaaataa attttaccct tgaaataaat ggctttttga
     1981 tcttgtgccg taaaagaata aacattatca ataagttttt tccatacata taattcagga
     2041 tcaacttgat gaacatttac tttgatataa taagtacgtt ctgtaacacc atcggaagcg
     2101 atatttttaa tttgagacac ttttgtgaaa tcaattggat ttccgctgga atatgttacc
     2161 agcgaatcat tatcgaaaat taaataacat gcggaagaac ttttaaagga aaatacggca
     2221 taaacactat caatacgtgt ctgataggga agtgaatcaa gatttacaat aatagaatca
     2281 tgaatcgttt cgtcaaacac caatgtaaaa actgcatttt ccaggttagg tacgctatca
     2341 ttttttaaaa atgaaagaga aacaaaacaa ggattattag aagtatctgt cacataatca
     2401 tctcctaaac aagaatttaa gaaaatgatc gctacacaaa aaatggacaa ctttaagtaa
     2461 ggagatggca taatatcaat atttattttc tttggacaaa gttaaaaaaa cattttattg
     2521 aagtattttc taaggacaaa gttttattat tttttaggga aatgttttat attttattca
     2581 agttcaatca ttaccggaca atggtcggaa tgaaatactt ctgaaagtat ttttgcagat
     2641 ttcagcctgg gcaataaagg tttgctaatc caatgataat caattctcca gcctgcattt
     2701 cgagagcgag cacctccacg aaaactccac caggtatatt ttcctggact ttgatcaaac
     2761 actctgaacg agtccacaaa accagcttct tgaaagcgat ccatccattc acgctcttcg
     2821 ggcaaaaagc ctgatacacc ttcctgaagt tctggatgat taatatcaat aggtttatga
     2881 cagatattga aatcaccaca caccacaaga ttgggacgaa gtttcaataa ttcctttata
     2941 aaaggtaaaa aatcttctaa aaattgcatt ttaaattgct gtcgttcgcc accagaagag
     3001 ccagaaggaa tataaacgtt caaaatggaa atatcttcaa aatctgttct gagtactctt
     3061 ccttctgcat cataaacagg attttgcatt cccatcacta cattatcagg tggaatttta
     3121 gtaagggtcg caacaccgct atatcctttt ttttcggcag aatgaatgta agtgatatat
     3181 cccaattcag cgaaaagcca ggtatcaatc tgatcaggtt gggcttttat ttcttgtaaa
     3241 caaagcacat caggattttc agcttgtaac cagtcggtaa aatttttcgt gagtgctgca
     3301 cgcaaaccat ttatattgta agaaataatt ttcattgaaa tttattaaaa aaaata
//
LOCUS       DAOTKS010000162         3350 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_23596_length_3350_cov_3.591806, whole genome shotgun sequence.
ACCESSION   DAOTKS010000162 DAOTKS010000000
VERSION     DAOTKS010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23596_length_3350_cov_3.591806"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="P5236_04100"
     CDS             complement(<1..1346)
                     /locus_tag="P5236_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005645163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HRU63575.1"
                     /translation="MKTRVGLFGIGLDTYWMQFDGLLEKLKIYQSEISKKIKNFGVEV
                     VDAGMVDNPLKASEAANYLKRNDVEIVFLYVSTYALSSTVLPVAQKLKVPIIILNLQP
                     VAALDYNMFNKLGDRGKMTGVWLEHCQSCSVPEIAGVFNRTNISYDIVSGYLQDEKAW
                     KEIKEWTEAAKVAKALRHHRMGILGHYYGGMLDVYTDLTRQSSFFGTHFEMLEMCELK
                     KYRDEVTPEELNTKIAEFHTAFDVSSECDPAEIERAAYTSIALDKLVTVHGLDSLAYY
                     YEGQVGNEYENMVTSVIAGNTLLTGKNIPVAGECEVKNAQAMKIMAEFGAGGSFSEFY
                     LIDFKDDVIYLGHDGPAHFAIAEGQVKLVPLPVYHGKPGKGLSIQMTVKHGPVTLLSV
                     VEGKEKIFLLVAEGKSVTGPTLEIGNTNSRYRFSLSAKDFIDRWSKQGPAHHCAIG"
     gene            complement(1805..>3350)
                     /locus_tag="P5236_04105"
     CDS             complement(1805..>3350)
                     /locus_tag="P5236_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63576.1"
                     /translation="DQCLKSLKELGVNVLQLSVAWGGKPANEVLNMEDLDEEQIAKWK
                     FRIAQCKKFGFKPLAHFGVPRVLNSDPVKPACISDPDVQQKYVQMMKKFLNTFPEVND
                     ILIYTYDQQAWICSEFGPCSRCSGIPISDRLPNFLNLLKKTMQDCRPQTQTTLWWKPW
                     ELSKGETIDILKKIDPHNFGLVLNSSSSNEVYPFNDRSFKSDLGVKRLVQYAFEHNIP
                     AIGEFDHTLYKGLYQIEDYFPRLIYEQMEGWKELKGVVGVKEYYGFAPSTYSVNYAML
                     HAWMKNPNASLDELLNQIAAPYGKKTAPLMIKAWEYVAQSVEAFPWDVTYMIGPMGLD
                     KSENGEHDWNCIKIINATWDTPIWESNRRANFMLTDSKVAHPWIFEDASLRLEDAAQL
                     SFEAVEYYNQAIAQNENNVADIIKQRDFVQHTGRSLRGKGIHFALTIASQDARTVAFD
                     KGQFEKVCKRIEQLLELDVNNGYKMAAIKLEEFKKDPKSWLKNNFYPLTWKSEAEPNW
                     SKWIAP"
BASE COUNT          994 a          710 c          523 g         1123 t
ORIGIN      
        1 atcccaatag cacaatgatg ggctggtcct tgttttgacc agcgatcaat gaaatcttta
       61 gcgctgagtg aaaatctata tcgactgtta gtatttccaa tttccagtgt aggtcctgtt
      121 actgacttcc cttcggctac cagtaaaaag atcttctcct ttccttctac aacagacaac
      181 aaggttactg gcccatgttt cacagtcatt tgaatagata accccttacc aggtttaccg
      241 tgataaaccg gtaatgggac cagctttacc tgtccttcag caatggcgaa atgggcaggt
      301 ccatcgtgtc cgagatatat tacatcatct ttaaagtcaa tcaaataaaa ttctgaaaat
      361 gatccacctg caccaaattc cgccatgatt ttcatggcct gggcattttt tacttcacat
      421 tcaccagcta cagggatgtt tttccccgta agcagtgtat ttccagcaat gactgaagtg
      481 accatatttt catactcatt gccaacttga ccttcataat aataagctaa cgaatcaagt
      541 ccatgaactg tcaccagttt atcgagagca atcgaagtgt aagcagcacg ttcgatctca
      601 gcaggatcgc attcggaaga cacatcaaag gcagtatgaa attcagcaat tttggtgttc
      661 aattcttctg gtgtaacttc atctctatac tttttcaact cacacatttc aagcatttca
      721 aaatgggttc cgaaaaaaga actttgtcta gtaagatcgg tatacacgtc aagcatacca
      781 ccataataat gtcccaatat ccccattcga tggtgacgaa gggctttcgc tactttggca
      841 gcttctgtcc attcttttat ctctttccat gctttttcat cttgaagata tccgctaact
      901 atgtcgtagg agatgttggt gcgattgaat acccctgcaa tttcgggaac cgaacaagac
      961 tgacaatgtt caagccaaac acctgtcatt tttccacggt cgcccaattt attgaacata
     1021 ttgtaatcca atgctgcgac aggctgaaga ttgagtataa ttatcggcac tttgagtttt
     1081 tgtgccacag gtaatacggt tgacgacaat gcatatgttg aaacatataa aaaaacgatt
     1141 tccacatcat ttcttttcaa gtagttagct gcttcactag cttttagagg attatcgacc
     1201 atcccagcat ccactacttc aacaccgaaa ttttttattt ttttacttat ttcgctttga
     1261 tatattttca acttctcaag caacccatca aattgcatcc aataggtgtc taaacctatc
     1321 ccaaataatc ctactctagt tttcattctt aaatcttaaa aattaataca gcttaaactt
     1381 tttctctaat acgagttcct atataattgt tttaatctac tttcttattt ttttgagaac
     1441 cttatggaaa aataaatcaa taatcatatt tttttggagt atattcctat ttcaaaaagt
     1501 ttgttactat gttaaaatgc tatcctacat ttaattagat aatgaatgca ttcatttatt
     1561 cttctgactt ctcgcggaac aaggggaatc atattaaaat caagacttac aagcaatcaa
     1621 attgtgttca taatatgtag tcagctccaa ctttaaattt attttttcaa aaatttaatt
     1681 atattcttcc tcaaaacata tcttgtggct tttcaatttc atagtatgga acaaatgtcc
     1741 agcataatca agcattttcg acactattca atcaatgatt atgggtttct gttacataat
     1801 tttattatgg agcaatccat ttactccagt ttggttcggc ttccgatttc cacgttaaag
     1861 gataaaaatt gtttttaagc caacttttag gatctttttt aaactcttca agtttaatgg
     1921 ctgccatttt atacccgttg tttacatcca attcaagcaa ttgttcaata cgtttacata
     1981 ctttctcgaa ttgacctttg tcaaatgcta cggttctggc atcttgtgag gcaatggtaa
     2041 gtgcgaagtg gattccttta ccacgcagcg aacgtccggt atgttgtaca aaatcccgct
     2101 gtttgataat atctgccaca ttgttttcat tttgagcaat ggcctgatta taatattcta
     2161 cagcttcaaa actgagttga gctgcatctt ccaatctcaa actagcatct tcaaaaatcc
     2221 atggatgagc cactttcgaa tcggtaagca taaaattcgc gcgacgattc gattcccaaa
     2281 taggtgtatc ccatgtagca ttaattattt ttatacaatt ccaatcatgt tctccatttt
     2341 cacttttatc taatcccatt ggtccaatca tataagttac atcccatgga aatgcttcca
     2401 cagattgtgc aacatattcc caggctttta tcataagagg agctgttttt ttaccatacg
     2461 gtgcagcaat ctggttcaac aattcatcca atgaagcatt tgggtttttc atccatgcat
     2521 gaagcattgc gtaatttact gaataagtag atggtgcaaa accataatat tcttttacac
     2581 ctaccacacc ttttagctct ttccaaccct ccatctgctc atatatgaga cgcggaaaat
     2641 aatcttcaat ttgatataaa cccttataaa gggtatgatc gaactcacct atcgctggaa
     2701 tattgtgttc aaaagcatat tgaacgagac gttttactcc taaatccgac ttaaatgaac
     2761 gatcgttaaa cggatatact tcattgcttg atgatgaatt aagcactaaa ccaaaattat
     2821 gtgggtctat tttcttcaaa atatctattg tctcgccttt tgaaagttcc caaggtttcc
     2881 accataaagt tgtttgagtt tgcggacgac aatcctgcat ggttttcttc aaaagattta
     2941 aaaaattagg tagcctgtca ctgatgggaa ttccactaca gcgagaacag ggaccaaact
     3001 cactacaaat ccaagcttgc tggtcataag tgtaaatcag tatatcattc acttcaggaa
     3061 aagtgttcaa aaactttttc atcatttgca catatttctg ctgaacgtca ggatcagata
     3121 tgcaagctgg ttttacagga tcactattaa gcactcgtgg cactccaaaa tgagccaatg
     3181 gtttaaatcc aaatttttta cattgagcta ttctgaattt ccattttgca atttgctctt
     3241 cgtcaaggtc ttccatgttc aaaacttcat tagctggttt ccctccccat gctacactta
     3301 actgaagcac attcacccca agttctttaa gacttttaag gcattgatcg
//
LOCUS       DAOTKS010000163         3336 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_23723_length_3336_cov_4.447120, whole genome shotgun sequence.
ACCESSION   DAOTKS010000163 DAOTKS010000000
VERSION     DAOTKS010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23723_length_3336_cov_4.447120"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(265..1149)
                     /locus_tag="P5236_04110"
     CDS             complement(265..1149)
                     /locus_tag="P5236_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HRU63577.1"
                     /translation="MKKKIGFIINPAAGNKNKNFIPDYIQQLLDLQLFKPEIVFTKYA
                     GEGSLLAKEFVNDGFSHVVAVGRDGTVNEIARSLIHTNTSLGILPIGSGNGLARHLKI
                     PLNIHKAIQLLNNSESINIDYGIVNQRLFFCTCGTGFDAYVATEFAKGRKRGIRAYFE
                     KVLKGYFSYHSQNYRLQGKNIDIEIKAFLLTFANASQWGNNAYIAPKASLQDGKMDIS
                     IMSNFPIFALPSIALQLFTKTISNDLFMTTLRTDEITVYRDTPGPFHYDGEPYEEAKE
                     IKIKMIPAGLKVLVRKRF"
     gene            1354..>3336
                     /gene="rnr"
                     /locus_tag="P5236_04115"
     CDS             1354..>3336
                     /gene="rnr"
                     /locus_tag="P5236_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HRU63578.1"
                     /translation="MSKKRKTSSHLPKKELIHYITTIFNQNPERTFNYRQIAAALNIH
                     SSSQRIYIRELLEELTEEDFLIEVSQGKFKANVRNKYVEGIVTRTGIKTYVIPDDGGD
                     KIFIPERKTNHALPNDRVKVLLYAHRHNQPLEGEVIEILERSQNTFVGVLEVSDNFAF
                     LVVDNHIMSNDIFIPKNKLKNGKSGQKAVVKIVDWTSEMKSPIGEVIDILGNKGENNT
                     EMHAILAEFGLPYQYPAEVEKAANKIDAGITPQEIAKRIDMRDVVTFTIDPREAKDFD
                     DALSIHQLENGLWEVGVHIADVTHYVQPNSMIDQEAQKRGTSIYLVDRTIPMLPERLS
                     NNICSLLPNEDRLTYSVIFQLNDKAEVINYQIARTVIRSKQRFTYEEVQKIIESGEGN
                     YKNEILKLNELAQILRKNRLNNGAIDFERVEVQFEIDKKGRPLSVFFKEQKEANQLIE
                     EFMLLANKTVATHIGKPQNGKKTKTFVYRIHESPDAQKLKDFASFIKKFGYHFKTSLP
                     KSKISNEMNKLLNDVQGKKEQNLIETLAIRSMAKAIYSTKNVGHYGLAFDYYTHFTSP
                     IRRYPDMMVHRLLTRYLANGRSVNTEEWEQYCEHSSDMEQLAAKAERESIKYKQVEFM
                     SERIGQIYNGIISGVTEWGIYVELIENKCEGMIPIRN"
BASE COUNT         1216 a          537 c          539 g         1044 t
ORIGIN      
        1 gaccattacg ttatagtgta ataaccgttc attttcactg aattttacag aaaaattcta
       61 ttttaccatc caaaatcatc tcacgtcagc aatttgttta aattttaata aaaaccttat
      121 ataaaaacat tgaaccctaa taactttgaa atatctcctt aattataacc ttattacctt
      181 atttagcttt cttgtggatc tttaaattgt taacttcaag attcacaata aacttaaagc
      241 taaaatacac gaaaattaag tgatttaaaa acgttttctc accaaaactt ttaagcctgc
      301 tggaatcatt ttaattttta tttcttttgc ttcttcataa ggttcgccat cataatgaaa
      361 aggtccaggg gtatcacgat aaacagtaat ttcatctgtt cgtaaagttg tcataaacaa
      421 atcattagaa atagttttag taaaaagttg aagtgcaatg gaaggcaagg caaaaatcgg
      481 gaaattactc ataatggaaa tatccatttt accatcttgc aggcttgctt ttggtgcaat
      541 ataggcatta ttaccccatt gagaagcatt agcaaatgtt aataagaaag ctttgatttc
      601 tatatcaata tttttacctt gtaaacgata attttgtgaa tgataactaa aatatccctt
      661 caaaactttt tcaaaataag ctcttattcc tcgttttctg cctttcgcaa attctgttgc
      721 tacataagca tcgaatccag tgccacaagt acaaaaaaat aatcgctgat ttactattcc
      781 ataatcaata ttaatagatt ctgaattatt caaaagttgg atagctttat gaatattcag
      841 gggaattttg aggtgacgag ccagcccatt gccagaacct attggaagaa tacccagcga
      901 agtgttggta tgtatcagcg agcgtgctat ttcattcact gtaccatcgc gaccaacagc
      961 caccacatga gaaaaaccat cattcacaaa ttcttttgct aacaatgaac cttcaccagc
     1021 atattttgta aaaacaattt ctggtttgaa aagctgcaag tctaataatt gctgaatata
     1081 atcgggaata aagtttttat ttttatttcc ggcagcaggg ttaataataa agccaatttt
     1141 ctttttcatc gtgactccat aaaatcccaa aattggtagt gaaattacaa ataatttttg
     1201 ggaacgaaaa aatcattttc aaagtaataa actattccat taattatttt gttgtaactt
     1261 tgcaaaattt tattaatctg ttgattcata tttgttttaa caaaaatata tttgaacatt
     1321 ctcttattta attatttttt ataactcatt tttatgtcca aaaaaagaaa aacaagtagt
     1381 catttaccta aaaaagaact aattcactat attaccacta tttttaatca aaatcctgaa
     1441 agaacattca attaccgaca aattgcggct gctttgaata ttcattcctc ttcacaacgc
     1501 atatatatcc gcgaattatt agaagaatta acagaagaag attttttaat tgaagtttcg
     1561 caaggaaaat ttaaagccaa tgtacgtaat aaatatgtag aaggcattgt tacacgtaca
     1621 ggcattaaaa catatgtgat accagacgat gggggagaca aaatctttat tcccgaacgc
     1681 aaaactaatc atgcattacc aaacgaccgc gtaaaagttc ttctatatgc tcacagacat
     1741 aatcaaccgc ttgagggaga agtaattgaa attttagaac gttctcagaa tacttttgtg
     1801 ggtgtgctgg aagtttccga taattttgcc ttcctggtgg tagataatca tattatgagt
     1861 aatgatatat ttattccaaa aaataaattg aaaaatggca aaagtggaca aaaagcagta
     1921 gtaaaaatcg ttgattggac atcagaaatg aaaagtccaa tcggtgaagt tattgacata
     1981 ttaggtaata aaggcgaaaa caacacagaa atgcatgcta ttttagccga gtttggtttg
     2041 ccttatcaat atcctgctga agttgagaaa gcggccaata aaattgatgc aggaataact
     2101 ccacaagaaa ttgccaaacg aatagatatg cgtgatgtgg taacctttac cattgatcct
     2161 cgtgaggcta aagattttga cgatgcttta tctatccatc aattagaaaa tggactatgg
     2221 gaagttggtg tacatattgc tgacgtcaca cattatgttc aaccaaacag tatgattgat
     2281 caggaagctc aaaaacgcgg tacttctatc tatctggttg atagaacaat tcctatgctc
     2341 cctgaacgac tttcgaataa tatttgttcc ttgttaccga acgaagatag gttaacttat
     2401 tccgtaattt ttcaactgaa tgacaaggcg gaagtgataa actatcaaat tgctcgtact
     2461 gtgatacgta gcaaacaacg ctttacttac gaagaagttc aaaagattat tgaatcggga
     2521 gaaggaaact ataaaaatga aattttaaaa ttaaatgaat tagcacaaat tcttcgaaaa
     2581 aatcgtttaa ataatggtgc aattgatttt gaacgggtag aagtacaatt tgaaattgac
     2641 aaaaaaggaa gacctctttc agtattcttt aaagaacaaa aagaagccaa ccagttaatt
     2701 gaagaattta tgctactggc aaacaaaaca gtggcaactc acattggaaa accacagaat
     2761 ggaaaaaaaa ctaagacatt tgtgtataga attcacgaat cgcccgatgc acaaaaactg
     2821 aaagattttg ccagctttat taaaaaattt ggttaccact tcaaaacttc cttgcctaaa
     2881 tctaaaattt caaatgaaat gaataaatta ttaaatgatg tacaaggaaa aaaggaacaa
     2941 aatttaattg aaacgttagc tattcgttct atggcaaaag ccatttattc tacaaaaaat
     3001 gtaggtcatt atggactggc tttcgattat tatactcatt tcacttcacc tattcgtaga
     3061 tatcctgata tgatggtgca tcgtttatta actcgctatc tggcaaatgg tcgcagtgtg
     3121 aacacagaag aatgggaaca atattgtgag cacagttccg atatggagca actggctgct
     3181 aaagccgaaa gagaatccat taaatacaaa caggttgaat ttatgtcgga aagaatagga
     3241 caaatttata atggaattat ttcaggagtt actgaatggg gtatttatgt ggagttaata
     3301 gaaaataaat gtgaaggaat gattccaatt cgcaat
//
LOCUS       DAOTKS010000164         3327 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_23804_length_3327_cov_4.917482, whole genome shotgun sequence.
ACCESSION   DAOTKS010000164 DAOTKS010000000
VERSION     DAOTKS010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23804_length_3327_cov_4.917482"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1634)
                     /locus_tag="P5236_04120"
     CDS             complement(<1..>1634)
                     /locus_tag="P5236_04120"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HRU63579.1"
                     /translation="LQKIAQQVRNAENKIIIAEKMGSLAYLKNNYHYKQDSIDEAWRT
                     LMLSQHHDSWIVPYNRLQKNTTWADKIAQWTETTDKIADSVVKEAVESFQGNNKSVND
                     DYSIRVFNTLGFNRNEIVSILLPETLKNKKVILLDTENQVLPSSVEEGQKISFLAQVP
                     AFGYATYKIQMGESKIKKEKKSFYSNKNECILENDKYRIVFDLQKGGTIKSLFSKNNN
                     KEYVKKGSEFLFGEMRGFFYEENKFHSSRENPAKVTLIKDNSLETSILIQGKIASQPF
                     KQIVTVNKEQERIDFDLVINWKQNLCIGEYAQPNNWQDNRRAFYDTRYMLSVLFPVDL
                     NSPQLYKNAPFDVCKSKLDNTFFNRWDSIKHNIILNWIDFSEVNGENSLALLTDHTTS
                     YSYGTDFPLALTIQYSGKGLWGRDYNITQPTHVKYALVPHKFRWDKSSISKKSSSWNE
                     PLIGVCSSDFDFENKSFIDLLSSGYELSSVQIMNDGILVRLFNSEGDGSAQKIKLCFP
                     FKSIEEMELNGEMKNANGSIKAILGNEIQLSIPRFGV"
     assembly_gap    1635..1734
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1735..2377)
                     /locus_tag="P5236_04125"
     CDS             complement(<1735..2377)
                     /locus_tag="P5236_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017142568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HRU63580.1"
                     /translation="MVKVKVRITWTLLVLSIFLNAENVYFVDGYHGGVYGHYPLEWET
                     RFIIDNFYKYPDWRICLEIEPETWDSVKVRTPQDYTQFKQILSDERIEFTNPAYAQPY
                     CYNISGESIIRQFQYGMRKINQHFPDVTFSTYSSEEPCFTSCLPQLLKSFGFQYAVLK
                     CPNTCWGGYTRAYGKDLVNWVGPDTTSILAVPRYQCESFEKNSTWQTIAWNNST"
     gene            complement(2370..2606)
                     /locus_tag="P5236_04130"
     CDS             complement(2370..2606)
                     /locus_tag="P5236_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63581.1"
                     /translation="MINLAIKYLNGELTSEEKERFFVLVAKDETLKNDLIKYHHLLAY
                     ISLLFRKEDNADTERRFLDLLNEIERKKRKEKDG"
     gene            complement(2683..3207)
                     /locus_tag="P5236_04135"
     CDS             complement(2683..3207)
                     /locus_tag="P5236_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HRU63582.1"
                     /translation="MERDFSNIYMTFYSPLVRFAMVFVIYKEEAENIVQDLFVGLWEK
                     KESLNCVENLKAYMFTLAKNKCLDYLKHKLVKENFIAKAQSFYEQEIALKAQSLESFD
                     VELASEEKVEKIVSEAIESLPPKCREIFILSRYEGLKYKEISERLNISVNTVETQMRI
                     AFKKLRNSINYLLN"
BASE COUNT         1041 a          592 c          449 g         1145 t
ORIGIN      
        1 acaccaaagc gaggaattga taattgtatt tcgttaccaa gaattgcctt tattgaacca
       61 tttgcatttt tcatttcacc attcaattcc atttcctcaa tgcttttaaa agggaaacac
      121 aatttaattt tctgagcact tccatctcct tcagaattaa aaagacgaac cagtattcca
      181 tcattcatta tctgaacgga acttaattcg taaccgctgc tcagcaaatc gataaaactt
      241 ttattttcaa aatcgaaatc ggaagaacaa acgccaataa gtggttcatt ccaactcgaa
      301 cttttttttg atatagaaga tttatcccac cgaaatttat gaggaacaag ggcatatttt
      361 acatgcgtag gttgagttat attataatcc cgtccccaca aaccttttcc cgagtactga
      421 atagttaaag ccaacggaaa atcagtgcca taagaatagg aagttgtatg atcggttaaa
      481 agtgccagac tgttttcgcc atttacttca gaaaaatcaa tccagttcaa tatgatatta
      541 tgttttatgc tgtcccaacg attaaaaaaa gtattgtcca atttactttt gcaaacatca
      601 aagggagcgt ttttataaag ttgtggcgaa ttaagatcaa ccggaaaaag aacacttaac
      661 atgtaacgcg tatcgtaaaa tgcccttctg ttgtcttgcc aattatttgg ctgagcgtat
      721 tctccaatac acaaattctg cttccaattt ataacaagat cgaaatcgat acgttcctgc
      781 tctttattaa cagtaacgat ttgcttaaat ggctgcgacg caattttacc ttgaattaaa
      841 atacttgttt ctaatgaatt atcttttatt aaagtaactt tcgctggatt ttcccttgat
      901 gaatgaaatt tattttcttc ataaaaaaaa cctctcattt ctccaaacag aaactcactg
      961 ccttttttta catattcctt attattgttt ttggaaaaca aacttttaat tgtgccccct
     1021 ttttgtaaat cgaaaacgat tctgtatttg tcattttcca gaatgcattc atttttattt
     1081 gaataaaaag atttcttttc cttctttatt ttcgattccc ccatttgaat tttataggtt
     1141 gcatatccaa atgcaggtac ttgagcaaga aaggaaattt tttgtccttc ttccactgaa
     1201 cttggaagga cttgattttc agtgtctaaa aggattactt ttttgttttt taatgtttcg
     1261 ggcaacagaa tagaaacaat ttcattgcga ttaaaaccca acgtattgaa aactctaata
     1321 gaataatcgt catttactga tttattgtta ccttgaaaac tttcaactgc ctctttaaca
     1381 acactgtcgg caattttatc ggtagtttct gtccattgag caattttatc tgcccatgtc
     1441 gtattttttt gaagtcgatt gtagggaaca atccacgaat cgtgatgctg agacaacatc
     1501 aatgttcgcc aagcttcatc aatactgtcc tgtttgtaat gatagttatt tttaagataa
     1561 gcaagagagc ccattttttc ggcaataatt atcttatttt cagcattgcg aacctgttga
     1621 gctatttttt gcaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaggttg
     1741 aattattcca agctattgtt tgccatgtgg aatttttttc aaaagattcg cattggtaac
     1801 gcggaacggc taaaattgaa gttgtatccg gcccaaccca atttaccaaa tctttcccgt
     1861 aagctcttgt ataaccgccc caacaagtat ttggacattt aagaacggca tattgaaaac
     1921 caaacgactt taacaattga ggcaaacagc tcgtaaaaca aggttcctct gaagaataag
     1981 tggaaaatgt tacatcagga aaatgttgat taatttttct catgccatat tgaaactggc
     2041 gaatgatact ttctccggaa atattataac aatatggctg agcataagct ggattagtaa
     2101 attctatcct ttcatcggaa agaatttgct tgaattgtgt gtaatcctgt ggtgttctaa
     2161 cttttacact gtcccaggtt tcaggttcta tctccaaaca tattctccaa tcaggatatt
     2221 tatagaaatt atctattata aatcgagttt cccattccaa tggataatgt ccgtatactc
     2281 caccatgata tccatcaaca aaatatacat tctccgcatt aagaaatata cttaaaacca
     2341 acagtgtcca agttattctt actttaacct taaccatcct tctcttttct ttttttcctt
     2401 tcaatttcat tcaacaaatc caaaaacctt ctttcagtat cggcattatc ttcctttcta
     2461 aagagtaaac taatataagc aagtaaatga tgatatttta taagatcatt ttttaaagtt
     2521 tcatctttcg ctactaatac aaaaaaacgt tccttttctt ctgaagttaa ttctccatta
     2581 agatatttta tggctaagtt tatcattcgt tcttttttaa ttaagaacga tttctaacct
     2641 gagttccatt aaaaaagaaa aaataaaatg ataactaatt ttctaattta ataaataatt
     2701 aatgctgttt ctcaatttct taaaggcaat acgcatttgg gtttccaccg tatttactga
     2761 aatgtttaat ctttctgaaa tttccttata ttttagtcct tcataacgac tcaatataaa
     2821 tatctcccga catttaggag gtagcgattc aattgcctct gaaactattt tttctacttt
     2881 ttcttccgaa gccaattcaa catcaaagct ttccaaagat tgtgctttta aagcaatttc
     2941 ctgttcgtaa aaggattggg ctttagcaat aaaattttct tttactaact tatgctttag
     3001 ataatcaagg catttattct ttgccaaggt aaacatatat gctttaaggt tttcaacaca
     3061 atttaaagat tccttctttt cccaaagccc tacaaataaa tcttgaacta tattttctgc
     3121 ctcttcttta taaatgacaa ataccattgc aaacctcacc aatggtgaat aaaatgtcat
     3181 atatatattt gagaaatccc tctccataat tttatggttt aatgatgaaa aactttaaaa
     3241 aaacagaaag gatgtttggg aacagtcagc taaaaataag cttgtaataa acgataatag
     3301 gaagtaataa agtttcatag tattatt
//
LOCUS       DAOTKS010000165         3320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_23867_length_3320_cov_3.809801, whole genome shotgun sequence.
ACCESSION   DAOTKS010000165 DAOTKS010000000
VERSION     DAOTKS010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23867_length_3320_cov_3.809801"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1307
                     /locus_tag="P5236_04140"
     CDS             114..1307
                     /locus_tag="P5236_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HRU63583.1"
                     /translation="MSSTTTTKPGRLLSLDVLRGFTIAGMILVNNPGSWQYVYAPLRH
                     AQWHGLTPTDLVFPFFMFIMGVSMYLSYRKFDFKLSKQTFWKFTRRSVLLFLIGLMLG
                     WFGLFCRELNQLNGVNMPLGEKILQAATQFENIRILGVLQRLALVSFFGTLIILLVRQ
                     KHIPWLIGFILLFYWILLGCTHSYEASVDNIIAVVDRAILGASHMYKETLADGSVIAF
                     DPEGILSTLPCMAHVLLGVWAGKLITETTNHEEKLQKLFIFGTILLFSGFLLDYGFPI
                     NKKIWSSSFVLVTCGFASLLLALLIWIIDIKQKKKWCVFFESFGVNPLFIYVVAGILS
                     ILLGNISFIYNGEWITIKNFVYTVWLAPVFGNYLGSLIYALCFVFINWIIGHILYKKR
                     IYIKL"
     gene            complement(1339..2268)
                     /locus_tag="P5236_04145"
     CDS             complement(1339..2268)
                     /locus_tag="P5236_04145"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRU63584.1"
                     /translation="MWKDFFYFSKSQRIGIIVLIILILLVIIANLTLPLLPKKPVNVN
                     DSTFFAEVAAFKKSLVSQDSLRKVEWEQRYKERYSSFKKFSSYSKNSYQLFPFDPNTA
                     DSVTFVRLGLKPYIATNILKYRAKGGKFKHPDDLAKIYGISEEKFQELLPYIKIDENL
                     FISSNKIDENKKESEKIFVIELNSADTTQLMQVKGIGRTYAQRIVRYRKSLGGFVSVD
                     QLKEVYGMTPENYNRILPYCVVDPDLIQKIKVNTANVDRLNQHPYLNFYQAKAIYELR
                     RKKGKLYDFKELKSIPEFSDDELEKIQPYLSFE"
     gene            complement(2469..>3320)
                     /locus_tag="P5236_04150"
     CDS             complement(2469..>3320)
                     /locus_tag="P5236_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRU63585.1"
                     /translation="YGIPEFRLPNYVVDVEVQNLKTLGVQFVTNCIVGKTLTLKDLED
                     EGYKGLFIGSGAGLPRFMNIKGENLVGVMSANEYLTRVNLMDAADPEAETPIFRGKKV
                     AVIGGGNTAIDAVRTARRLGAEKAMIIYRRTEQEMPARIEEVKHAKEEGIEFMMLRNP
                     KEYIGDEKGRVIQMLLQVMKLGEVDDSGRQTPVPVIGETELIDVDEVIISIGVSPNPL
                     IPQSVKGLEVTKRGKLVVNNETMQATIPNIFAGGDIVRGSATVILAMGDGRRAAKGMD
                     RWLKNQQ"
BASE COUNT         1015 a          532 c          516 g         1257 t
ORIGIN      
        1 ttcttctaaa tttgtgacct gagtttttta aaaaattgaa aattccattt ctccgatttt
       61 taccttttat tttttgtcca ttaagttaaa gttgttttta aaatttctga aatatgtcat
      121 ccacaactac cacaaaacca ggacgtttac tttcgttaga tgtattaaga ggatttacaa
      181 ttgcaggtat gattttagtt aataatcctg gaagttggca atatgtttat gcacctttac
      241 gtcatgctca gtggcatggc ttaactccga cagatttagt ttttcctttc tttatgttta
      301 ttatgggagt ttccatgtat ttatcgtacc gaaaatttga ttttaagctt tccaaacaaa
      361 ctttttggaa atttacacgt cgttcagtat tactcttttt aattggttta atgttgggat
      421 ggtttggtct tttttgtcga gaattaaatc aactgaatgg tgtcaacatg ccgttaggtg
      481 agaaaatttt acaagctgct actcagtttg agaacatacg aattttaggt gttttacagc
      541 gtcttgcgtt agtctcgttt tttggaactc ttatcattct tttagttcgt caaaagcata
      601 ttccttggct tattggtttt attttactgt tttattggat tctgttaggc tgtactcata
      661 gttacgaggc tagtgttgat aatataatag ctgttgtaga tagagcaata ttaggagcat
      721 ctcacatgta caaagaaact ttggctgatg gtagtgtaat tgcctttgat cctgaaggta
      781 ttttaagcac tttaccttgt atggcacatg tattattagg tgtgtgggcg gggaaactta
      841 ttacggaaac aactaatcat gaagaaaaat tacaaaaatt atttattttc ggaaccattc
      901 ttttgtttag tggcttttta cttgattatg gtttcccaat taataaaaaa atctggtcat
      961 caagttttgt attggttact tgtggctttg cttcattgct gcttgctttg cttatttgga
     1021 ttattgatat aaaacaaaaa aagaaatggt gtgtgttttt tgaatcgttt ggtgttaatc
     1081 cgttgtttat ttatgtagtt gcagggatat taagtatatt attagggaat ataagtttca
     1141 tttataatgg agaatggatt accatcaaaa attttgtata tactgtttgg ttagcacctg
     1201 tttttggaaa ttatttgggt tccttgattt atgctttatg ttttgttttc attaattgga
     1261 ttattggtca tattctttat aaaaaaagaa tttatattaa attatagaaa aacataactt
     1321 gtttttatta ctgaataatt attcaaaact taaataaggt tggatttttt ctaattcatc
     1381 atcagaaaat tcaggtattg atttcaactc tttaaaatcg tataatttgc ctttctttcg
     1441 tcgaagttca taaatagctt tggcttgata aaaattgaga tagggatgtt gattcaagcg
     1501 gtcaacattt gcagtattaa ctttaatttt ttgaatcaaa tcaggatcaa cgacacaata
     1561 aggaagaatt cggttataat tttctggcgt cataccatat acttccttta attgatcgac
     1621 ggaaacaaaa ccgcctaaac tttttcgata acgaacaatt ctctgagcat aagttcgacc
     1681 aatacctttt acttgcatta attgtgttgt atctgctgaa tttaattcta tgacaaaaat
     1741 tttttctgat tcttttttat tttcgtctat tttatttgaa gaaataaata aattctcatc
     1801 aattttaata taaggcaata gttcctggaa cttttcttca gaaattccat agatttttgc
     1861 taaatcatca ggatgtttaa attttccacc ttttgctcta tattttaaaa tattagtggc
     1921 aatataaggt tttaaaccaa gacgaacaaa ggtaacagaa tcggcagtat ttggatcaaa
     1981 tggaaagagt tgataactat ttttggaata ggaactaaat tttttaaatg aactataacg
     2041 ttctttatag cgttgttccc attcaacttt acggagacta tcttgtgaga ccaaagattt
     2101 tttaaatgct gctacttctg caaaaaaagt agaatcattg acgttaacag gttttttagg
     2161 taataaagga agagttaaat tagcaattat gacgagtaaa attaaaatga tcagtacgat
     2221 aatacctatt cgttggcttt tagaaaaata aaaaaagtct ttccacatcc tcagaatcag
     2281 ttaaaagaat tattggtttt acttataatt ttactctttt aataacgtac cacaaagttg
     2341 atataaaatt ttttcaggtc attataaaat caagtaaaaa tgaaaaaata tttttttact
     2401 ttatcatatt gattttgaat gatttctttt caattatttc aaataaaaat atgggaagaa
     2461 ggaatgaatt attgttgatt ttttagccat cgatccatcc ctttggcagc ccgtcgtcca
     2521 tcacccatag caagaataac tgtggcacta ccgcgaacaa tgtcgccacc ggcaaaaata
     2581 ttaggaatag tggcttgcat agtttcatta ttcaccacca gttttccccg tttagtaact
     2641 tccaatcctt ttaccgattg tggaatcaaa ggattaggtg aaacaccgat actaataata
     2701 acttcatcta catcaataag ttctgtttct cctattacag gtacaggagt ttgtcttcca
     2761 gaatcatcaa cttcacctaa tttcattact tgcaataaca tttgaataac tctacctttt
     2821 tcatcgccaa tatattcttt tggattacgg agcatcataa attcaatgcc ttcctctttg
     2881 gcgtgtttca cctcttcaat acgtgcaggc atttcttgct ctgttctccg atagataatc
     2941 atagcttttt ctgctcctaa tcttcgagcc gtacgtacag catctatagc cgtatttcct
     3001 ccaccaatta cagcaacttt ttttcctcta aaaattggag tttcagcttc tgggtctgca
     3061 gcatccatta aatttactcg cgttaaatat tcattagccg acattactcc tactaaattt
     3121 tcacctttaa tattcataaa acgtggcaaa cctgctccac ttccaataaa aagaccttta
     3181 tacccttcat cctccaagtc tttaagtgta agcgttttgc ctacaatgca atttgttaca
     3241 aactgaactc ccaaagtttt caagttttga acttcaacat caactacata attgggcaaa
     3301 cgaaattcag gaattccata
//
LOCUS       DAOTKS010000166         3314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_23926_length_3314_cov_4.532065, whole genome shotgun sequence.
ACCESSION   DAOTKS010000166 DAOTKS010000000
VERSION     DAOTKS010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23926_length_3314_cov_4.532065"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="P5236_04155"
     CDS             complement(<1..355)
                     /locus_tag="P5236_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRU63586.1"
                     /translation="MSSFKIEGGQKLSGTIVPQGSKNEALQVICATLLTSEKVIISNV
                     PHILDVKLLMNLLIQLGVEIEKLQENTYSFKAENIKLNYLKTKDFYQKSSLLRGSVMI
                     VGPMLARFGEAIFPKP"
     gene            complement(416..1000)
                     /locus_tag="P5236_04160"
     CDS             complement(416..1000)
                     /locus_tag="P5236_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HRU63587.1"
                     /translation="MEYPTLKGKLIMPEYGRNIQQMISYAMTIEDREERTRCVKTIVN
                     IMGNLFPYLRDVSDFKHKLWDHVAIMSNFQLDIDFPYEILRPDDLHTRPEPIPYKNSQ
                     IRYRHYGRTLEEMIEKVADYPEGEERNELIRLIANQMKKCFLTWNKETVDDRKIFDDL
                     RELSGGKIDISEDFLKLVESREVLHSKKSKQKRK"
     gene            1253..1969
                     /locus_tag="P5236_04165"
     CDS             1253..1969
                     /locus_tag="P5236_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRU63588.1"
                     /translation="MGRAFEYRKATKFKRWGNMARVFTKLGKQITIAVREGGPDPDMN
                     PRLRVLMQQAKKENMPKENIERAIKKAISKDEIDSYKEVLYEGYAPHGIAMIIETATD
                     NPTRTVANIRSYFNRYGGSLSTSGSVQFMFDHKCVFRIAPQINTSLEDLELELIDYGV
                     DEVDQDDDSIVLYGDFPSYYSIQKYLEDHNFEIYSAQFEWIPKDMKELNDDQKAQVIK
                     LIEKIEEDDDVQNIFHNMIE"
     gene            complement(2034..2852)
                     /gene="panB"
                     /locus_tag="P5236_04170"
     CDS             complement(2034..2852)
                     /gene="panB"
                     /locus_tag="P5236_04170"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRU63589.1"
                     /translation="MSIINENVRKVTTRRLLEMKQRGEKISMLTAYDYTMANIVDEAG
                     IDVVLVGDSASNVICGNTTTLPITLDQMIFLAKSVVRGVKRALIVVDMPFGSYQGNSR
                     EAIASAIRIMKETHADGIKIEGGEEILESVHRILSAGIPIMGHLGLMPQSINKYGSYT
                     VRAQEEEEAKKLIRDAHLLEEAGCFSIVLEKIPAKLGAQIASELTIPVIGIGAGGGVD
                     GQVLVIHDMLGLTQGFSPRFLRRYANLREIMHNAIQHYIEDVKSGDFPNENEQY"
     gene            complement(3121..>3314)
                     /locus_tag="P5236_04175"
     CDS             complement(3121..>3314)
                     /locus_tag="P5236_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63590.1"
                     /translation="LYNAERDKINNIFKEAIPYFKKAVELKPDDIDYKRNLRSLYYRL
                     QMDKEAAEIDEEIKAMENN"
BASE COUNT         1130 a          551 c          514 g         1119 t
ORIGIN      
        1 ccggtttagg gaaaatggct tctccaaacc gagccaacat cggtcctact atcattacag
       61 aacctctaag caaagagctt ttttgataaa agtcttttgt tttgagataa tttaatttta
      121 tattttcagc cttaaaggaa taggtatttt cttgtagttt ctcaatttca actccaagtt
      181 gaatcagaag attcattagc aacttaacat ccaatatatg aggaacatta ctaataatca
      241 ccttttctga agttagtaaa gtagcacaaa ttacctgcaa tgcttcattt ttggagcctt
      301 gtggaacaat ggtgcctgaa agcttctgac ctccttcaat tttgaatgaa gacataaaat
      361 aaagatagct aaatttatac ctttctactt tataatctcg aaaaatgaca gataattatt
      421 tccttttctg cttacttttt ttcgaatgaa gtacttctct tgattcaact agtttaagaa
      481 aatcttctga aatatcaatt tttcctcctg ataattcgcg taaatcatca aaaatctttc
      541 gatcgtccac tgtttcctta ttccacgtaa gaaaacattt tttcatctga ttagcaatca
      601 gacggattaa ttcatttctt tcttctcctt ctgggtaatc tgctactttc tcaatcattt
      661 cttccaaggt acgtccataa tgacgataac gaatttgaga atttttataa ggaatgggtt
      721 ccggacgagt atgtaaatca tcaggtcgta aaatttcgta agggaaatca atatctagtt
      781 gaaaattaga cataatggct acatgatccc ataatttgtg tttgaaatcg ctcacatctc
      841 gtaaataagg gaataaattg cccataatgt ttacaattgt ttttacgcat cgagtacgtt
      901 cttctcgatc ctcaatagtc attgcataag aaatcatttg ctgaatattt cgtccatatt
      961 caggcatgat taattttcct tttaaagttg gatattccat catagatgaa gttttataat
     1021 tgacaaaaat aatgaattgt ttgttgagaa aaaagcatat tgaaaaaaat cattgtcttt
     1081 tgtagttaaa attgatttca gattttttgt aaacataatt ccataagtga taaagactca
     1141 tgataaaagt aatcattcgg aaagagagtt attttacata attggaaaaa ttctctaaat
     1201 ttgcatctat aaatgattca gataataagt aaacgaatat aaattattac gtatgggaag
     1261 agcatttgag taccgaaaag caacgaaatt taaacgttgg ggtaatatgg cccgggtatt
     1321 tacaaaattg gggaaacaaa ttaccatagc agtccgtgag ggtggacctg atcctgacat
     1381 gaatccaaga ttgagagttt tgatgcagca agcaaaaaag gaaaacatgc ccaaggaaaa
     1441 tatcgaaaga gcgataaaaa aagcaatctc caaagatgaa atagatagtt ataaagaagt
     1501 actttatgag ggttatgccc ctcatggaat agccatgata atagaaacag caaccgacaa
     1561 tccaacacga acagtagcaa acatccgaag ttattttaat cgttatggtg gctcgttaag
     1621 tacttcgggc tctgtacaat ttatgtttga tcataaatgt gtttttagaa ttgctccgca
     1681 aattaatact tctctcgaag atctggaatt agaattaatt gattatggtg ttgatgaagt
     1741 agatcaggat gacgacagta ttgtgttata tggtgatttt ccttcttatt attctattca
     1801 gaagtatttg gaagaccata attttgaaat ttatagtgcc caatttgaat ggattccaaa
     1861 agacatgaag gaattaaatg acgatcagaa agctcaagta ataaagttga tcgaaaaaat
     1921 tgaagaagat gatgatgtcc aaaatatttt ccacaatatg atagaataaa atttctgttt
     1981 catcaaataa tattttttat tgaattttta ttcggatttt aaaaaattta tcttcaatat
     2041 tgttcatttt cattggggaa atcgccagat ttaacatctt caatataatg ctgaatggca
     2101 ttgtgcataa tttctcgcaa attagcatat ctacgaagaa aacgtggcga aaatccttgt
     2161 gtcagcccta acatgtcatg gataaccaat acctgaccat ctactcctcc gccagcacct
     2221 atgcctatca caggaattgt aagttctgaa gctatttgag cccctaattt tgcgggaatt
     2281 ttttccagca caatagaaaa acaacctgct tcttcaagta agtgagcatc tcttattaat
     2341 ttttttgctt cttcttcttc ttgagccctt actgtgtaag aaccatattt attaatagat
     2401 tgtggcatta aaccaagatg ccccataata ggaatgccag cacttaaaat tcgatgaact
     2461 gattcaagaa tttcttcacc tccttctatt tttatcccat cagcatgagt ttctttcata
     2521 atgcgaatag ctgatgcaat agcttccctt gaattccctt gataacttcc aaaaggcata
     2581 tctactacta tcaaagctcg ttttacacca cgcaccactg atttagccaa aaaaatcatt
     2641 tgatccaaag taataggcaa agtagtagta tttccacata ttacatttga agctgaatcc
     2701 ccaactaaaa ccacatcgat tcctgcttca tcaacaatgt ttgccatagt ataatcataa
     2761 gcggtaagca tgctgatttt ttctcctcgt tgcttcattt cgagcaaacg acgagtggtc
     2821 acttttctga cattttcatt aattatagac attattaata atgtgtttaa aataatgtgc
     2881 aaacttacaa aaaaattaaa aagaacagtg ttaaaaaatg aataggtaaa atattatata
     2941 ttatatagtc attccttaaa aatagacttt ttaaggaatg actatatata aatggctatt
     3001 tcgaaaaagc cagtcaagga caaataataa cagccataga aatcaagaag attttgaaac
     3061 aaaaaaggca tctggaaaat attagatgcc tttatatttt gtaatttaga cgaaaaaaaa
     3121 ttaattgttt tccattgctt taatttcttc atcaatttct gctgcttctt tgtccatttg
     3181 caatctgtaa tacaaagagc gtaaattacg tttataatct atatcatctg gtttcaattc
     3241 cactgctttt ttaaaatagg gaatagcttc tttgaatata ttgtttattt tgtctctctc
     3301 ggcattatat agtt
//
LOCUS       DAOTKS010000167         3303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_24028_length_3303_cov_4.413177, whole genome shotgun sequence.
ACCESSION   DAOTKS010000167 DAOTKS010000000
VERSION     DAOTKS010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24028_length_3303_cov_4.413177"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="P5236_04180"
     CDS             complement(<1..1054)
                     /locus_tag="P5236_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRU63591.1"
                     /translation="MKFEYFIAKRIHFQQGKKNVSRPAVRIATIGIALGLAVMLISVC
                     VVIGFKSEVRNKMIGFGGHIQITNFDNNNTYEMIPIQMDSLLIQKISEIKGVKGIQKF
                     ATKPGIIKTADEFQGIILKGVDKNYDWNFFKSNLVEGSIPDLFSETPKNEILISRYLA
                     NLLKLKLDDQFYTYFLQDQIRARKFKIVGIYSTNFVDYDKMFLITDIRHVQRLNNWSS
                     DMFGGLEVLIDDFDHLDEIGEKVTFLTANKFTKEGNAYFTQTIKQLNPQIFSWLDLLD
                     MNVWVILALMMAVAGFNMISGLLILILERTSMIGILKAMGATNWQIRKIFIYHSFFLV
                     GKGMLWGNFIGLAICAL"
     gene            complement(1158..2360)
                     /locus_tag="P5236_04185"
     CDS             complement(1158..2360)
                     /locus_tag="P5236_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRU63592.1"
                     /translation="MPHTSQRGLSMPESPIRKLAPLADKAKARGIKVYHLNIGQPDLK
                     TPQKALDVLKTIDRTILEYSPSDGFKSLKIKLASYYEKFNIDVTEEDIIITTGGSEAV
                     NFAFMSCLDPGDEIIVPEPAYANYTAFAIAAGVVVRPVISTIDDGFALPSIEKFEELI
                     TPKTKGIMICNPNNPTGYLYTRSEMNQIRDLVKKYDLFLFSDEVYREFCYSGAPYISA
                     FHLNGIEENVILADSFSKRYSECGIRVGALVTKNKEVRKNVMKFCQARLSPPLIGQII
                     AEASIDTPQEYMLESYNEYLERRKFLIDGLNRIPGVYSPIPMGAFYTVARLPVDDADK
                     FCEWMLSDFEYEGATVMLAPGSGFYTLEGMGKDEVRIAYVVNKEDLKKSLIILAKALE
                     VYPGRTNK"
     gene            complement(2518..>3303)
                     /locus_tag="P5236_04190"
     CDS             complement(2518..>3303)
                     /locus_tag="P5236_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRU63593.1"
                     /translation="GNVNVALNSHIKSPRSVHFVLGGDYYFRALGRPFKFTTETYLKL
                     TDRLISYSIDNVKIVYSGKNDGKAYTAGIDFKLFGELVPGTDSWINFSLMRSREDLIN
                     DSYVKNTYDENGNLSSSEIVNPGWIPGPNEQRYSFSMMFQDYLPTDPRYKLQLKFIWS
                     DGLPFGPPQNYQFRSVFRMPPYRRVDIGASRLLVNGKDKWMEKTWLKPVKDIWLNVEV
                     FNLLDFKNVNSYYWVTDVYGQQLAVPNYLTGRQFNIKLIVDFK"
BASE COUNT         1109 a          636 c          499 g         1059 t
ORIGIN      
        1 gtaaagcaca aattgccaat ccaatgaagt tcccccacaa cattcctttt cctaccagaa
       61 aaaaagaatg ataaataaat atttttctta tttgccaatt ggttgctccc attgctttta
      121 gtatgccaat catgctggta cgttcaagaa tcaaaataag caaacctgaa atcatgttga
      181 aacctgctac agccatcatt aaagccagaa taacccaaac attcatatcc agcaaatcga
      241 gccagctaaa aatttgagga ttcaactgtt taatagtttg agtaaaatag gcgttccctt
      301 ctttcgtaaa cttatttgca gtaagaaaag ttactttttc acctatttca tccagatgat
      361 caaaatcatc aatcaatact tccaacccgc caaacatatc tgatgaccag ttatttaaac
      421 gttgaacatg acgaatgtca gtgattaaaa acattttatc ataatccaca aaatttgtag
      481 aataaatgcc tacaatttta aattttcttg ctcgaatctg atcttgcagg aaataagtat
      541 aaaattgatc atctaatttc aattttaata aattggcaag atatctggag ataagaattt
      601 catttttagg tgtttccgaa aataaatccg gaatgctccc ttcgaccaga tttgatttaa
      661 aaaaattcca atcataattt ttatctacac cttttaaaat gataccttga aattcgtcag
      721 ccgttttaat tattcccggt ttggtagcga acttttgaat accttttact ccttttatct
      781 ccgaaatttt ttgtatcagc aacgaatcca tttgaatggg aatcatttcg taagtattat
      841 tattgtcaaa attagtgatt tgaatatgac cgccaaaacc aatcattttg ttgcggactt
      901 cacttttgaa accaataacc acacaaacgg aaatcaacat cacagccagt cccaatgcaa
      961 ttccaatggt agcaatccga actgctggac gagaaacatt tttctttcct tgttgaaaat
     1021 gaatgcgttt tgctataaaa tattcgaatt tcaaatcagc gaataatttt caaaatatta
     1081 cctttttgaa acaggccaga aattgggaaa attataaagc aagaatggaa aaattccaaa
     1141 taattgattt tcaaccatca tttatttgtt cttcctggat atacttccaa tgctttagct
     1201 aaaataatca aagatttttt caaatcctcc ttatttacta cataggcaat ccgtacttca
     1261 tccttgccca ttccttccag cgtataaaaa ccagaacctg gtgccagcat aacagtagct
     1321 ccttcgtact caaaatcaga aagcatccat tcacagaatt tatcagcatc atcaacaggc
     1381 aagcgagcta cggtataaaa cgctcccatt gggataggcg aataaacgcc cggaatgcga
     1441 ttcagtccat caattaaaaa tttacgtcgt tccagatatt cattgtaaga ttcaagcata
     1501 tattcttgtg gtgtatcaat tgaggcttct gcaataattt gcccaattaa tggcggactt
     1561 aatcgagctt gacaaaattt cattacattt tttctaactt ctttgttttt ggtcaccaac
     1621 gctcctacac gaataccaca ttcgctgtaa cgttttgaaa atgaatcggc cagaataaca
     1681 ttttcctcaa taccatttag atgaaaagct gaaatataag gtgctccact atagcaaaat
     1741 tcgcgataaa cttcgtcaga aaacaaaaat aaatcatatt tttttactaa atcacgaatt
     1801 tgattcattt ccgaacgcgt atacaaatag cctgtcggat tattcggatt acaaatcatg
     1861 atgccttttg ttttcggtgt aatcaattct tcaaattttt caatggaagg caaagcaaaa
     1921 ccgtcatcaa tggtggaaat gacggggcgt acaacgacac ctgcagctat ggcaaaagct
     1981 gtataattgg cataagcagg ttctggcact ataatttcat cgcccggatc caaacaactc
     2041 ataaaagcaa agttgaccgc ttctgaacct cctgttgtga taataatatc ttcttctgta
     2101 acgtcaatat taaatttttc ataataactc gccaacttga tttttaaact tttaaaacca
     2161 tcacttggac tgtattccaa aatagtgcga tcaattgttt ttaaaacatc taaagctttt
     2221 tgtggagttt tcaagtcggg ctgacctata ttcaaatgat aaactttgat tcctcgtgct
     2281 ttggctttat cagccagtgg agcaagttta cgaataggag attcaggcat ggaaagccca
     2341 cgttgtgaag tgtgtggcat ttttaaaggt gttattttga tattttatga tagtttttat
     2401 tctaaatatt ttaaaaaaaa aaaatttagt tacaaattta gcaaaacttt acagaaaatt
     2461 ctatttgctc tgaaattaaa atcgaaacgg atactggaag tatttttgtt tttattttta
     2521 tttaaaatcc acaatcaatt ttatattaaa ttgtcttcca gtcagataat tgggaacagc
     2581 caattgctgc ccatacacat ccgttaccca ataataggaa tttacatttt taaaatccag
     2641 taaattaaat acttccacat tcaaccagat atctttgaca ggtttcagcc aggttttttc
     2701 catccattta tcttttccat ttaccaacaa gcgagatgct ccaatatcca ctcgacgata
     2761 aggtggcatt ctgaaaactg agcgaaactg ataattttgt ggcggaccaa atggcaaacc
     2821 atctgaccag atgaatttta attgcaattt atagcgagga tcggtaggca aataatcctg
     2881 aaacatcatg gagaaggaat aacgttgttc gttgggtcct gggatccagc ccggattaac
     2941 tatttcagaa gaagataaat ttccattttc gtcataagta ttttttacat aactatcgtt
     3001 aattaaatct tctctcgacc gcattaacga aaaattaatc caggaatctg ttcctggaac
     3061 caattccccg aaaagtttaa aatcaattcc cgcagtataa gctttaccgt catttttgcc
     3121 agaataaaca atttttacat tgtcaataga ataagaaatg agtcggtcgg tgagtttgag
     3181 atacgtttcc gtagtaaatt taaaaggtcg tcccaatgcc ctgaaataat aatcacctcc
     3241 caatacaaaa tgcacagaac ggggcgattt gatatgagaa tttaatgcta catttacatt
     3301 tcc
//
LOCUS       DAOTKS010000168         3289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_24146_length_3289_cov_3.717687, whole genome shotgun sequence.
ACCESSION   DAOTKS010000168 DAOTKS010000000
VERSION     DAOTKS010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24146_length_3289_cov_3.717687"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2535)
                     /locus_tag="P5236_04195"
     CDS             complement(<1..2535)
                     /locus_tag="P5236_04195"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HRU63594.1"
                     /translation="MKAKKYTSDEVMEASLKYFQGDDLAAKVWTTKYALKDSFGNFYE
                     LTPDDMHHRLAREIARIEKKYPQPLSEEELFELLRNFKYIVPQGSPMSGIGNNFQIAS
                     LSNCFVIGFDHDADSYGGIIKIDEEQVQLMKRRGGVGHDLSHIRPKGSPVKNTALTST
                     GIVPFMERYSNSTREVAQDGRRGALMLSISIKHPDAESFIDAKMEANKITGANISVKI
                     DDEFMKAALEGKPYIQQFPIDEPEPRVKKEIDAATLWKKIIHNAWQSAEPGVLFWDTI
                     IRESIPDCYADLGFKTISTNPCGEIPLCAYDSCRLLAINLYSYVDRPFTSEASFNFEL
                     FKKHVALAQKIMDDIIDLEIENIDQILSKIKSDPEDEEIKRTEYMLWEKIRAKTLQGR
                     RTGVGTTAEGDMLAALGYRYGTTEAIDFSEHVHKILALSAYRSSVTLAQERGAFSIYD
                     SKREENNPFIHRIRQADPDLYNDMVKYGRRNIACLTIAPTGTTSIMTQTTSGIEPVFM
                     PVYTRRRKVNPNDKNVQIDFVDDNGDSWEEYTVFHHKFVTWMEANNYPTTKKYSKSEI
                     DDLVAKSPYYKATANDINWLKKVQMQGRIQKWVDHSISVTINLPAETTEELVADLYEE
                     AWRCGCKGVTVYREGSRSGVLVSMDEAQKEKQEEKKVCFPVTEGPLVRPKELECDVVR
                     FQNAKDKWIAFVGLKDGRPYEIFTGLADDEEGISLPKTVTHGKIIKTVDENGNKRYDF
                     QFTNKRGYKTTVEGLSEKFDKEFWNYAKLISGVLRYGMPIDQVVKLVSGLQLDSDSIN
                     TWKVGVERALKKYIPDGTQADGQTCPSCKQKTLIYQEGCLTCKNCGY"
     gene            complement(2838..>3289)
                     /locus_tag="P5236_04200"
     CDS             complement(2838..>3289)
                     /locus_tag="P5236_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HRU63595.1"
                     /translation="NGKESIQLNYLSHHQQYDIKIRLKETREKDKILGFSTQGIHKDE
                     LEMLLDGYPIKRVGSQGQNKTFLISLKLAQFNFLSHTHQQFPILLLDDIFDKLDTDRV
                     KRILELVSQESFGQIFITDTDREHLNQILSQLQQEIKIFKVENGTIC"
BASE COUNT         1000 a          608 c          558 g         1123 t
ORIGIN      
        1 atagccacaa tttttacatg tcaagcagcc ttcctgataa atgagtgttt tctgtttaca
       61 cgaagggcaa gtttgtccat ctgcctgagt gccatcagga atgtattttt tcaaagctct
      121 ttctacacca actttccatg tgttaatgga atcactatcc agttgcaatc ctgaaacgag
      181 tttgactacc tgatcgatag gcattccata acgcaacacg cctgaaatta attttgcata
      241 attccagaat tctttgtcaa atttctccga caatccttca actgtggttt tgtaacctcg
      301 tttattggta aattgaaaat catatcgttt gttaccattt tcgtcaactg ttttaatgat
      361 tttgccgtga gtaacagttt taggaagtga aattccttct tcgtcatcag ctaaaccggt
      421 gaaaatttcg taaggacgcc catcttttaa tcctacaaaa gcgatccatt tatcttttgc
      481 attttgaaaa cgaactacat cgcattctaa ctcttttgga cgaaccaacg gaccttccgt
      541 cacagggaaa caaacttttt tctcctcttg tttttctttt tgggcttcat ccatcgaaac
      601 gagaacgcct gaacgcgagc cttcacgata aaccgttact cctttgcagc cacatcgcca
      661 ggcttcttca tataaatcag ctaccagttc ttcggtagtt tcagcaggta aattaatggt
      721 aacactaatg gaatgatcca cccatttttg aattctgcct tgcatttgta cttttttaag
      781 ccagttaata tcattggctg tggctttata ataaggtgat ttggcaacta aatcatcaat
      841 ttcactttta gaatattttt ttgtggttgg atagttattg gcttccatcc aagttacaaa
      901 tttgtgatga aaaacagtgt attcttccca cgaatcgcca ttatcatcta caaaatcaat
      961 ttgcacattt ttatcattcg gatttacttt tctacggcga gtataaacag gcataaatac
     1021 aggttcaatg ccggatgtcg tttgcgtcat aatgctggta gttccagttg gagctatggt
     1081 taaacaagca atattccgac gaccatattt taccatgtca ttgtacagat cgggatcggc
     1141 ttgacgaatg cgatgaataa aaggattgtt ttcctctctt tttgaatcat aaatactaaa
     1201 agctcctctt tcctgagcca gcgttaccga agaacgataa gcacttaaag ccagtatttt
     1261 atgaacgtgt tctgaaaaat caatggcttc tgttgttccg taacgataac ccagtgcagc
     1321 aagcatatca ccttcagcag tagttcctac gccagttcgt cttccttgca aagttttagc
     1381 acggattttt tcccataaca tgtattctgt cctttttatt tcttcatctt caggatctga
     1441 tttgatttta gacagaattt gatcgatatt ttctatttcg agatcaataa tatcgtccat
     1501 gattttttga gccagagcaa catgtttttt gaaaagctca aaattaaagc tggcttcaga
     1561 agtaaagggt cgatcaacat aagaatacaa attaatggct aataatcggc aactatcata
     1621 agcacataag ggtatttccc cgcatggatt ggtagaaatg gtcttgaatc ccaaatcagc
     1681 ataacagtca ggaatagatt ctcgaataat ggtatcccag aataacacgc ccggttcagc
     1741 agattgccag gcattatgaa tgattttttt ccataaagta gctgcatcga tttctttctt
     1801 tacgcgtggt tctggttcat caatgggaaa ttgttgaata taaggttttc cttccaaagc
     1861 tgccttcata aattcatcgt ctattttaac agaaatgttg gctcctgtaa ttttattggc
     1921 ttccattttg gcatcaataa atgattctgc atcaggatgt ttaatagaaa tagaaagcat
     1981 taaagcacca cgacgaccat cttgagcaac ttcgcgagta gaattggaat aacgttccat
     2041 aaaaggaaca atgcctgtag aagttaaagc tgtatttttt acaggagaac ctttgggacg
     2101 gatatgagat aaatcgtgtc ccacgccacc acgacgtttc atcaattgta cctgttcttc
     2161 atcaatttta atgatgccac catacgaatc tgcatcatga tcgaaaccaa ttacaaaaca
     2221 attggacaga gaagcgattt gaaaattgtt cccaatgcca ctcataggac ttccttgagg
     2281 aacaatatac ttgaaattgc gtaataattc gaacagttct tcttcggaaa gtggttgagg
     2341 atactttttt tctatccggg caatttcgcg agccagccga tgatgcatat catcaggtgt
     2401 caattcgtaa aagtttccaa aggaatcttt caaggcatat tttgtggtcc agactttagc
     2461 agccagatca tctccttgaa agtattttaa actcgcttcc attacttcgt ctgaagtata
     2521 tttttttgct ttcacgataa tatgttttaa taattagatt aagcaataag ggtttatatt
     2581 caaaaatgac aataaaatta tctatttgca atgttataaa ttatttttaa attgtacaat
     2641 ttttataaca ttattttttc aaaatattta taattatcta tttaaaaaat tatattattg
     2701 atttacaatg aaatacaaat ttaaaatagt taaaatattt cccaaaatag aatttcttaa
     2761 aaaataagga atgaattatt tttcaattta tcggattaga taacttgctg aaaagaaaaa
     2821 atcattaagc aatttgatca acaaattgtt ccattttcta ctttaaaaat ttttatttct
     2881 tgctgaagtt gggaaagaat ttgattgagg tgttcacgat ctgtgtccgt aataaaaatt
     2941 tgtccgaatg attcttgcga aacaagttcc aggattcgtt taacgcggtc tgtatccagt
     3001 ttgtcaaaaa tatcatccaa taagagaata ggaaattgct gatgagtatg tgacagaaaa
     3061 ttgaattgag ctaattttaa agaaatgaga aaagttttat tttgcccttg tgaacctact
     3121 cgtttaatgg gataaccgtc cagtagcatt tccagctcat ctttatgaat cccctgcgta
     3181 gagaatccaa gtattttatc tttttcgcgg gtttctttca gccgaatttt tatgtcgtat
     3241 tgctgatgat gcgaaagata attaagttga atagattctt ttccgtttg
//
LOCUS       DAOTKS010000169         3280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_24220_length_3280_cov_5.318760, whole genome shotgun sequence.
ACCESSION   DAOTKS010000169 DAOTKS010000000
VERSION     DAOTKS010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24220_length_3280_cov_5.318760"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..2343
                     /locus_tag="P5236_04205"
     CDS             145..2343
                     /locus_tag="P5236_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HRU63596.1"
                     /translation="MSSLRFKAVEEAFKKKAINVEPPSALVSDYFGKYVFTRSKMAEY
                     LSEETMKAIDEAIEKGVTLDRSIADDVAAGMKKWAIELGATHYTHWFQPLTEKTAEKH
                     DSFIDYVEDKPCEKVIEKFSGKLLAQQEPDASSFPSGGIRSTFEARGYTAWDPSSPAF
                     VMDDTLCIPTIFISYTGESLDYKAPLLKALNAVDKAATAVCQLFNSKVKKVYSYLGWE
                     QEYFLVDENLYAARPDLMLTGRTLLGHESAKNQQLEDHYFGAIPTRVMAYMKDLEFEA
                     YKYGIPLKTRHNEVAPNQFELAPIYNECNLSVDQNLLLMSIMKKVARRHSFRLLLHEK
                     PFAGINGSGKHCNWSLGTDIGMGLFTPGKTPEENLLFITFVVNVLMAVYKNNSLIKAS
                     IMTAQNAHRLGANEAPPAIISVFLGEQLSNILDKLEMSDSEDIIIFDHKKKLNLKIPQ
                     IPEVLLDNTDRNRTSPFAFTGNRFEFRAMGSSANCSTAMIVLSTVLAAQLLDFKSEVD
                     TKMEAGLAKQQAIFEVIKEYIQISKPIRFEGNNYSEEWKVEAKKRGLDCEMSVPKILK
                     AYLKPESVHIFSFLGVLSEKELYARNEVKLETYTKKIQIESRVLGDLAVNHIIPVAIR
                     YQTLLAENISKIKNITFDNNGKDICKMQLELIREIAQRVNIIKENVNEMIEARKKANI
                     IENEEEKANAYHDTVLPYFDVIRYHIDKLEMIIDDEMWPLPKYREILFIR"
     gene            2423..2986
                     /locus_tag="P5236_04210"
     CDS             2423..2986
                     /locus_tag="P5236_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63597.1"
                     /translation="MENNDWKEKTQQFQKEIEKEIAELEVTMKQLMDGLNEKYGSQVE
                     NLKQQAKATQAKIDEKIAQIQAKMNDSTDEMKRKYADQITELKQKEELIQKEVDKKIA
                     ELGKKMSESADELKTKYEEQIKELKKMKDDLLYKMNNWSEVTEKNFRALTEVNEKTFK
                     EISQTLKQSFSEIKNLFSSSQEKDSSV"
     gene            complement(3063..>3280)
                     /locus_tag="P5236_04215"
     CDS             complement(3063..>3280)
                     /locus_tag="P5236_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63598.1"
                     /translation="ILFYKKLKATWWATILFFPLQYYFGKFAFFWHKEFKKLKAKIQY
                     LTLKVRQSIVLERTKFLHDQIIHLIEI"
BASE COUNT         1242 a          442 c          594 g         1002 t
ORIGIN      
        1 aatgagtttt tcaaacattt tttataattt cgcacttgct ggttgactct actctaatga
       61 ttttttattc cgaaatttgt caaataaaaa acaatatcat tattttgtaa gtttgtttat
      121 tacaattata taacatacaa gtttatgtca tcattaagat ttaaagcagt agaagaggct
      181 tttaagaaaa aagctattaa tgtagagccg ccatcggcat tggtttccga ttattttgga
      241 aaatatgtat ttactcgttc aaaaatggca gaatatcttt cagaggaaac catgaaagct
      301 attgatgaag ccattgaaaa aggagtaacg cttgatagaa gcattgctga tgatgtggca
      361 gcagggatga aaaaatgggc tatcgaatta ggagctaccc attatactca ttggtttcag
      421 ccattaacgg agaaaactgc agagaaacat gattctttta ttgattatgt agaggataaa
      481 ccatgtgaaa aagtaattga aaaattttca gggaaattat tggcacaaca ggaacctgat
      541 gcttcttctt ttccgagtgg cggtattcga agtacttttg aagcacgagg ttatactgcg
      601 tgggatcctt cttcgcctgc ttttgttatg gacgacacac tgtgtattcc aaccattttt
      661 atttcttata caggagaatc acttgattat aaagcgccac tgttaaaggc tttaaatgct
      721 gttgacaaag ctgcaactgc tgtatgtcag ttgtttaact caaaagtcaa gaaagtttat
      781 tcatatttgg gctgggaaca ggaatatttc ttagtagatg aaaacttgta tgctgctcgt
      841 cccgatttaa tgttaacagg acgaacatta ttgggacatg agtcagctaa aaatcagcaa
      901 cttgaagacc attattttgg agcaattcct acacgtgtaa tggcatacat gaaagatctg
      961 gaatttgaag cttataaata tggtattcca ttaaaaacac ggcataatga agttgcccct
     1021 aatcaatttg aactagcccc catttataat gaatgcaact tgtcagttga tcaaaattta
     1081 ctattaatgt caataatgaa aaaagttgca cggcggcata gtttcagatt actcttgcac
     1141 gagaagcctt ttgctggaat aaatggttcg ggcaaacatt gtaactggtc attgggtacc
     1201 gatatcggaa tgggattatt cacacccgga aaaactccag aagaaaatct tttgtttatc
     1261 acttttgttg tcaatgtttt aatggcagtt tataaaaata attcattgat taaagcttcg
     1321 atcatgactg ctcaaaatgc tcaccgatta ggagcaaacg aagcacctcc agctattata
     1381 tcggtatttt taggtgaaca actttcaaac atactggata aactggaaat gagcgatagc
     1441 gaagacatca ttatttttga tcataagaaa aaattaaatt taaaaattcc tcagattcct
     1501 gaggttttat tagacaatac agacaggaac agaacttcac cttttgcctt tacaggaaat
     1561 cgttttgaat ttagggcaat gggctcgtca gccaattgtt ccacagctat gattgttctc
     1621 agcacagttt tagcagctca actgcttgac tttaaatcag aagtggatac aaaaatggag
     1681 gcgggattag ctaaacaaca agcaattttt gaagtaataa aagagtatat acaaatttcc
     1741 aaacctattc gttttgaggg aaataattat tcggaggagt ggaaagttga agcaaaaaaa
     1801 agaggtttgg attgtgagat gagtgttcct aaaatattaa aagcttacct taaaccggaa
     1861 agtgtccata ttttttcttt tttgggtgtt ttatcggaaa aagaacttta tgctcgtaat
     1921 gaggtaaaac tcgaaacata tacgaaaaaa attcagattg aatccagagt tttgggtgat
     1981 cttgctgtaa atcatattat ccccgttgct attcggtatc aaactttatt ggcagaaaat
     2041 atttctaaaa tcaaaaatat aacttttgat aacaatggaa aagatatttg caaaatgcaa
     2101 ctggaattaa ttcgtgaaat tgctcaacga gttaatatta ttaaagaaaa tgtaaatgaa
     2161 atgatagaag caagaaaaaa ggcgaatatt attgaaaatg aagaagaaaa agcaaatgct
     2221 tatcacgata cggtattgcc ctattttgat gtaatacgtt accatattga caaattggaa
     2281 atgattattg atgatgaaat gtggcccttg ccaaaataca gagagatact gtttattaga
     2341 taggagaaga ataggtataa aaaatgagta agttattata ataaaaaatt gtaaaaatgt
     2401 ttcatttaat attatagaaa atatggaaaa caatgattgg aaagaaaaaa cgcagcaatt
     2461 tcagaaagaa attgaaaaag aaattgctga attggaagtc acaatgaaac aattgatgga
     2521 tggtttgaat gaaaagtatg gatctcaagt ggagaattta aaacagcagg caaaagcaac
     2581 tcaagcaaaa attgacgaaa aaattgcgca gattcaagct aaaatgaatg attccactga
     2641 tgaaatgaaa agaaaatatg cagatcaaat tacagaatta aaacaaaaag aagaactaat
     2701 tcaaaaagaa gtagataaaa aaattgcaga gcttggaaaa aaaatgagcg aaagtgccga
     2761 tgaattgaaa acaaaatatg aagagcaaat taaagaatta aaaaagatga aagatgattt
     2821 actttataaa atgaataatt ggagtgaagt aacagaaaag aattttcgag ccttaacaga
     2881 agtaaatgaa aaaactttta aagaaatatc gcaaacttta aaacaaagtt tttcagaaat
     2941 aaaaaatctt ttttcttctt cgcaagaaaa ggactcttca gtttaaattt ttttagtctt
     3001 actttaaagc cttatttgaa ttaaaaaatt tagataaggc tttttataaa aaaaatttat
     3061 cgttatattt caatcaaatg aatgatttgg tcatgtaaaa atttggttcg ttctaaaacg
     3121 atggactgtc gtactttaag tgtaagatat tgaattttag cttttaactt tttaaattct
     3181 ttatgccaga aaaaagcaaa ttttccaaaa taatattgta aaggaaaaaa taaaatagtt
     3241 gcccaccacg tagctttaag ttttttataa aataaaatgg
//
LOCUS       DAOTKS010000170         3280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_24222_length_3280_cov_4.280620, whole genome shotgun sequence.
ACCESSION   DAOTKS010000170 DAOTKS010000000
VERSION     DAOTKS010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24222_length_3280_cov_4.280620"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            486..1718
                     /locus_tag="P5236_04220"
     CDS             486..1718
                     /locus_tag="P5236_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HRU63599.1"
                     /translation="MILNYIWIGFFLIAFVVAFIQMIVYGNVQVFSDMLNATFDSAKT
                     GFEISLGLTGVLSLWLGIMKVGERGGVVQAFSKVVSPFFEKIFPDLPKNHPAYGSMLM
                     NIAANMLGLDNAATPLGLKAMKEMQETNPKKDTASNPMIMFLVLNTSGLTLIPVTIMV
                     YRAQLGAANPSDIFLPILIATFFSTLAGFLSVAAYQKINLLDKTILKTLLGLIIIIGS
                     ILYFFSKVTQNQLTVYSNFIASFILISLIISFIVAAMVHKINVYDAFIEGAKEGFQVA
                     IGIIPYLIAILVAIGVFRAAGAMDMLMNGFAWFFGLIGVNTDFVGALPTALMKPLSGS
                     GARGMMVDAMKMYGADSFVGRLSCIIQASTDTTFYILAVYFGSVGIKNTRYAVICGLL
                     ADFVGIVAAILIGYLFFH"
     gene            1775..3103
                     /locus_tag="P5236_04225"
     CDS             1775..3103
                     /locus_tag="P5236_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRU63600.1"
                     /translation="MKMQEVIEYIIRFLLGDESSTEIANLIGYTSDKEEFSHYKLVIY
                     PSDFFQPEVYGTPQTLPVLPLKIWEDTPILFGQPKIEKIGQTRILYADIIASTYFLIT
                     RYEEMIRPECRDVHGRFPGKESISYRAGFIDRPLVDEYGKLLRKQLRELGIDVPEPSK
                     KIKKIFLTHDVDQLAHYRRFRGMMGALWRGIKQPNKRPEAKRAIKTFFKGIRFDPWYT
                     FPWLFKMDLSLIDILGKERCEIIVFIKAGGGKQREDKPHSNLVHPDYQNLIKYCKRKN
                     ITIGLHTSYKAGKYPELIAEEKKRLERNAKVKIYYNRNHFLRMCEPEDMETLIQAGIT
                     DDFTLTYADVAGFRIGTCRPVKWINPKNKELTSLTLHPMTIMDSTLSDKRYMNMSAYD
                     AYEYCVQLINNIENYGGEFVLLWHNTMVEEKSQSYHRTLYQNIIDYLKTK"
BASE COUNT         1082 a          461 c          578 g         1159 t
ORIGIN      
        1 atgcgttgaa ctaaataagc aagggtactt tgttcgtatt ttcttaataa attggttcgc
       61 gttcttcctt cagaaattgt tttgacaatt tcttgttgct tttcatcttt aatttttccc
      121 atttttatgt agtggaaaga ataattttta aatgaggttg cttataccgc aaaagtagta
      181 ttttttacaa ctctatgcaa ttttttgaaa caataatcta taattttaaa aaataaaaca
      241 taagacaata tcaaaacttt tgtaattcaa ttagttggaa aaaataataa attatttcta
      301 aaaattatct aactttatat agtttgttaa tctaatttct caaataaata ttaagatttt
      361 tagctttgtt atcaataata aaatgaaaga gaaggtaaaa actaaaaaat aatgtatttt
      421 tgcagcagaa caataattaa tgatgacatt actggtattc aattttcttc caaaaagata
      481 attatatgat tttaaattat atatggattg gatttttttt gattgctttt gttgttgctt
      541 ttattcaaat gattgtttat gggaatgttc aggtgttttc tgatatgctc aatgctactt
      601 ttgattcagc aaaaacaggt tttgaaattt ctttgggttt aacaggtgtc ctttctttgt
      661 ggttgggaat tatgaaagta ggtgaacgcg gcggcgtggt tcaagctttt tctaaagtag
      721 tttcaccttt ttttgaaaaa atttttcctg atcttcctaa aaatcatcca gcttacggaa
      781 gtatgttgat gaatattgct gcgaatatgt tgggattgga taatgcagct actccactcg
      841 gactaaaagc tatgaaagaa atgcaggaaa ccaacccaaa aaaagatact gcttccaatc
      901 ctatgattat gttccttgtg ttaaatactt caggattaac tttgatccct gtaacaatta
      961 tggtttatcg agcccaattg ggagcagcta atccgtccga tatttttctt cctattttga
     1021 tagctacctt tttctcgaca cttgcaggat ttttaagtgt ggcggcttat caaaaaatca
     1081 atttactgga caaaactatt ttaaaaactt tattagggtt aataattatt attggttcta
     1141 ttttgtactt tttcagtaaa gttactcaaa atcaacttac tgtatattca aactttattg
     1201 ccagctttat cttaatttct ctgatcattt ctttcattgt ggctgcaatg gttcataaaa
     1261 taaatgtata tgatgctttt attgaaggtg caaaagaagg ttttcaggtg gcaattggta
     1321 ttattccgta tttgattgct attttagtgg caattggcgt gtttcgtgct gctggtgcta
     1381 tggacatgtt gatgaatggt tttgcctggt tttttgggct aataggtgtt aatactgact
     1441 ttgtaggagc cttacccact gccttaatga aacctttaag tggcagtggt gcaagaggaa
     1501 tgatggtaga tgcaatgaaa atgtatggtg ccgattcttt tgttggcaga ctttcctgca
     1561 tcattcaagc ttctactgat accacttttt atattctggc tgtttatttt ggatcggttg
     1621 gcattaaaaa tactcgttat gcggttattt gtggtttatt agctgatttt gttggaattg
     1681 ttgcagctat tttaattgga tatttatttt ttcactgaaa aaatttaatt taaaaactga
     1741 ttttcagtta acgacataag acaaagtaat ttttatgaaa atgcaagaag taattgaata
     1801 tataataaga tttttattag gtgacgaaag ttcaactgaa attgctaatt tgataggata
     1861 tacttctgat aaagaagaat ttagtcatta taagctcgtt atttatcctt ctgacttttt
     1921 tcaaccagag gtttatggaa ctcctcaaac tttgccagtt ttaccattaa aaatttggga
     1981 ggatacacct attctttttg gacagccgaa gattgaaaaa attggacaga caagaattct
     2041 ttatgcagat ataattgcca gcacctattt tttaattaca cgttatgaag aaatgattcg
     2101 gccagaatgt cgagatgttc acggacgctt tcctggaaaa gaatccatta gttatcgggc
     2161 tggttttatt gatcgtccat tggttgatga atatggaaaa cttttacgga aacaattaag
     2221 agaattaggg atagacgtgc ctgaaccctc aaaaaaaata aagaaaatat ttttaacaca
     2281 tgatgttgac caattagctc attatcgtag gtttagagga atgatgggag ctttatggag
     2341 aggaatcaaa caacccaata aacgacctga agctaaaaga gccataaaaa ctttttttaa
     2401 aggaataaga tttgacccgt ggtacacttt tccatggctt tttaaaatgg atttatcatt
     2461 aattgatatt ttgggaaaag aaagatgtga aattatcgtt tttataaaag ctggtggagg
     2521 aaaacaaagg gaagacaaac ctcattcaaa tttagttcat cctgactatc aaaatcttat
     2581 aaaatattgt aaaagaaaaa atattacgat tggcttgcat accagttaca aagcaggtaa
     2641 gtatccagaa ttaattgcag aagagaaaaa aagattagag cgaaatgcga aagtcaaaat
     2701 ttactataat cgtaatcatt ttttgaggat gtgcgagcca gaagatatgg aaacacttat
     2761 ccaggcagga ataactgatg attttacatt aacttatgct gatgtggctg gttttagaat
     2821 tggaacatgt cgtcccgtaa aatggattaa tcctaaaaat aaggaattaa cttcactgac
     2881 ccttcatccc atgactatca tggacagcac gttaagtgat aaacgctata tgaatatgag
     2941 tgcttacgat gcttacgaat attgcgtaca attaatcaat aatatagaaa attacggagg
     3001 agaatttgtt ttattgtggc ataacacaat ggttgaagaa aaatctcaat cctatcatcg
     3061 cactttatat caaaatatta ttgattatct gaaaacaaaa taaaatgcaa aaaacagcca
     3121 tagtttgcgt aaccaatgat ttaaccacag atcaacgtgt tcataaaacc tgtctaactt
     3181 tacaaaaatg tggttatcaa actattgaag tcggtcgaat gttgccccaa agtttacctt
     3241 tggaaagacc ttattctgtt catagattta aactctggtt
//
LOCUS       DAOTKS010000171         3275 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_24274_length_3275_cov_3.486957, whole genome shotgun sequence.
ACCESSION   DAOTKS010000171 DAOTKS010000000
VERSION     DAOTKS010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24274_length_3275_cov_3.486957"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="P5236_04230"
     CDS             <1..290
                     /locus_tag="P5236_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63601.1"
                     /translation="IIDKRINATDAIGFSGILVYTDFDGKYCAFDLCCPYEAQQNVKI
                     KPNDIGEAVCEKCGSVFSIGHGIGDPISGPAKQPLKRYKTFVQGDVLYIVN"
     gene            complement(306..1316)
                     /locus_tag="P5236_04235"
     CDS             complement(306..1316)
                     /locus_tag="P5236_04235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRU63602.1"
                     /translation="MPDFTPGETNKNILSVSVIVPCHNEEKNLLSLFEALSVQTYQDF
                     ELLLINDHSTDATESIIKNHLPIFKNAVYFSSKEKGKKKALQEAIELAKGELIITTDA
                     DCTPSNKWIETIIDFYSQQNCDLIIGPVKMKNDSSFFQRLQTLEFVSLIATGATTAAS
                     GKPILCNGANLAFLKKSFLESKNDLHDEELSGDDIFLLQSIKKRQGKIQFLKFTEAFT
                     ETQACKTIAEFFKQRKRWASKSKTYQDKFTVFIALLVFLMCFNILVLLIVSIMQPIFW
                     KIFITIFIFKYLLDASFLATVSKFFSIKNMWRNAFFLSLFYPFYVVLVTIAGIVFKQK
                     KW"
     gene            complement(1515..1934)
                     /locus_tag="P5236_04240"
     CDS             complement(1515..1934)
                     /locus_tag="P5236_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRU63603.1"
                     /translation="MIYHFNSYQYLIKHSIRPSVQRTAIMDYLINNRNHPTVDEIYLA
                     LVPGMPTLSKTTVYNTLNLFVERGAVQVLNIDEKNARFDINTSIHAHFICSRCGKIQD
                     IFEISPENLEIKENHDFVVAKVEISYHGICKDCLSSN"
     gene            complement(2121..3062)
                     /gene="mdh"
                     /locus_tag="P5236_04245"
     CDS             complement(2121..3062)
                     /gene="mdh"
                     /locus_tag="P5236_04245"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HRU63604.1"
                     /translation="MSKVTVVGAGNVGATCVNVLAYNHIVDEVVMLDVKNGIAEGKAL
                     DMIQTATLLHFNTRITGCTNDYAQTANSDVVVITSGVPRKPGMTREELVEVNAGIVKE
                     VAEKILKFSPNAILVVVSNPMDTMTYLTYKATGLPRNRVIGMGGTLDTARFKHYLSIA
                     LDANPNEIEALVIGAHGDTSMIPLVRYASYKCRPVTELLDKDTLNKVVSDTMLGGSTI
                     TNLLGTSAWYAPGAAAAFLVEAIIHDERKIIPCSVYLEGEYGQNDICLGIPALIGKNG
                     IEKILQYSLNYEERELFRKSAASVHETNNTLKKLVEI"
BASE COUNT         1112 a          516 c          501 g         1146 t
ORIGIN      
        1 taattattga taaacgaatc aatgcaacgg atgccattgg atttagtggt attttggttt
       61 atacagattt tgatgggaaa tattgtgctt tcgatttgtg ttgcccgtat gaagctcagc
      121 aaaatgttaa aatcaaaccg aatgatattg gagaagctgt ttgcgaaaaa tgtggcagcg
      181 ttttttcaat tggtcatgga attggcgatc ctatttctgg ccctgccaaa caaccattaa
      241 aacgttataa gacttttgtt caaggcgatg tactttatat tgtaaattaa ttaattttca
      301 gaattttacc actttttttg tttgaataca attcctgcta ttgtgactaa caccacataa
      361 aatggataaa ataacgatag aaaaaaagca tttctccaca tgtttttaat ggaaaaaaat
      421 ttgctaactg ttgctaaaaa actagcatcc aacaaatatt taaaaataaa aatcgtaata
      481 aaaattttcc aaaaaatggg ttgcataatt gaaactatca gcaaaactaa aatattaaag
      541 cacattaaaa ataccagcaa cgcaataaaa acagtaaatt tatcctgata agttttcgat
      601 ttggaagccc aacgttttct ttgtttgaaa aattcagcaa tcgttttaca agcttgtgtt
      661 tctgtaaaag cttccgtaaa ttttagaaat tgaatctttc cttgtcgttt ttttatgctt
      721 tgcaataaaa aaatatcatc gcccgacaat tcttcatcgt gtaaatcatt tttactttcc
      781 agaaatgatt tttttaagaa agcaagatta gctccattac aaagaatcgg tttccctgaa
      841 gctgcagtag ttgctcctgt ggcaattaag gaaacaaatt caagggtttg cagtcgttga
      901 aagaaggaag aatcattttt catttttaca ggtccaatga ttaagtcaca attttgttgg
      961 gaataaaaat ctataatggt ttctatccac ttgtttgatg gagtacaatc ggcatcagta
     1021 gtaataatta attctccttt agctaattca atggcttctt gtaaggcttt tttcttacct
     1081 ttttcttttg aagaaaaata aacagcattc ttaaaaattg gaagatgatt ttttataata
     1141 ctttctgtgg catcagtgga atggtcattg atcaacaata attcaaaatc ttgataagtc
     1201 tgaacggata aggcttcaaa taaagaaagc aagttttttt cttcgttgtg gcagggaacg
     1261 atgacagaaa ctgaaaggat atttttattg gtctcaccag gagtgaaatc aggcaatttt
     1321 ttccaccctt ttataaaaaa aatgatcagt aggaaatata aaacactgat aaccaaaagg
     1381 taaatataga attccatgct caagcagcaa aagatacaat ttttcttttt ccaactttat
     1441 gatctttagt agaaattcgt gacggataaa tcaaacttct aagttttcag atataaaaaa
     1501 atagcgaaaa tttattaatt tgaagataaa caatctttac agattccatg gtacgaaatt
     1561 tccacttttg ctacaacgaa atcatggttt tcttttattt caagattttc gggactaatt
     1621 tcaaaaatgt cttgtatttt tccgcaacga gaacaaataa aatgagcatg gatagaagta
     1681 ttaatatcaa aacgggcatt tttctcgtct atatttaata cttgtacggc acctcgttct
     1741 acaaataaat taagtgtgtt gtaaactgtt gtttttgata aagttggcat tcctggcacc
     1801 agtgcaagat aaatttcatc gacagtggga tggtttctgt tgttgattaa atagtccatt
     1861 attgctgtac gttgaacaga aggtctgata gaatgcttga ttaaatattg atagctattg
     1921 aaatgataaa tcatggataa ttttttataa tggatacaga attagtaaaa ataatcagtt
     1981 tttgagattt tgtaatggag ttcttcctca cttaactatc ggctttaaaa atagaagttt
     2041 gattagtaaa atttcgtcaa atgaaaaaat atagttttga acataacctt tttcacttga
     2101 ttattatata ttatacttaa ttaaatttca acaagttttt ttaaagtatt attggtttca
     2161 tgtaccgaag ctgcactttt cctaaataat tctctttctt cataatttaa tgaatattgg
     2221 agaatttttt caataccatt ttttccaatt aatgcaggta ttcccaaaca aatatcattt
     2281 tgtccgtatt caccctctaa atataccgaa catggaatta tttttctttc gtcgtgaatg
     2341 attgcttcta ctaagaatgc tgcagcagct cctggtgcgt accacgcaga agtgcccagt
     2401 aaattggtaa ttgtagaacc tcctaacata gtgtcagaaa ctactttatt caatgtgtct
     2461 ttatctaaaa gttcagttac aggtctacat ttataagaag cataacgaac caaaggaatc
     2521 atcgaagtat cgccatgagc tccaattacc aatgcttcaa tttcgttcgg atttgcatca
     2581 agagcaatac ttaaataatg tttaaaacgt gcagtatcaa gagttccacc catcccaata
     2641 actctatttc ttggtaagcc tgtagctttg taagttaaat aagtcattgt atccatagga
     2701 ttacttacta ctacaagaat agcattaggt gaaaatttta aaattttttc agctacttct
     2761 tttactattc ctgcatttac ctctacgagc tcttcgcgag tcattccagg tttacgaggt
     2821 actcctgaag taatcactac tacatctgaa ttggcagttt gtgcataatc gtttgtacag
     2881 cctgtaattc tcgtattaaa atgaagcaaa gtggcagttt gaatcatatc cagtgcttta
     2941 ccttctgcta tgccattttt tacatctaac attactactt catctactat atggttataa
     3001 gcaagcacat taacacaagt ggcacctaca ttaccagcac caacaacagt tacttttgac
     3061 atattatttt attaaaatta attcaatttt ttatagtgac tgcaaaggta ataattaaat
     3121 aagttaaaga aaaatctttg cttgtttttc attttttttg tagcacccgt ataaaccttt
     3181 gaatatcaat actaattttg tattttggag attataaaaa aagcgtaaaa aaacaagagt
     3241 agaaaacaat ttctcccttt ttacataaga ataat
//
LOCUS       DAOTKS010000172         3271 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_24312_length_3271_cov_4.308147, whole genome shotgun sequence.
ACCESSION   DAOTKS010000172 DAOTKS010000000
VERSION     DAOTKS010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24312_length_3271_cov_4.308147"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="P5236_04250"
     CDS             complement(<1..1108)
                     /locus_tag="P5236_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRU63605.1"
                     /translation="MKLSQIATVLNAKVKKNIPDVEINWLLTDSRTVSFPSESLFFAI
                     KTQRNDGHKYIGELYEQNVHYFVVSEMRPEFESYEKTIFLFVKDTLEALQRLAAFHRN
                     SFQIPVIGITGSNGKTVVKEWLYQLLRFDYHITRSPRSYNSQIGVPLSVWALNENTQL
                     GIFEAGISQPHEMERLELMIRPTIGIFTNLGEAHQENFSSLKQKCEEKLKLFTHTEVL
                     IYCCDNKLVDITIAQSNFKGKLFSWGRNEKAVVRVLSIEKQSNTTEISLKYQNHEDSF
                     TIPFIDDASIENGLQCISLMLYLNYDVGTIKKRVLQLEPVEMRLEVKQGIHNCLIIND
                     TYNSDLNSLTIALDFLNQQAASKDLSRTLILSDIL"
     gene            complement(1113..2957)
                     /locus_tag="P5236_04255"
     CDS             complement(1113..2957)
                     /locus_tag="P5236_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRU63606.1"
                     /translation="MKKRIFLVVYLFICFFIVGFAQRPFSFALLTDLHISATNPQASE
                     DLQMVIDEINTNDQIEFILVSGDITNYGDKLSLEMAKRILDQLKNPYYIVPGNHEMKW
                     SESGATDFAKIFGDDKFSFTYNDCYFIGFATGPIIKMGDGHVAPQDIEWTKKQLKKIG
                     KNNPIFVVTHYPLLNGDVDNWYEVTDVIRRYNVQAVLGGHYHRNVFLNYDEIPGIIHR
                     STLRGNEPKGGYSILNVSDSIYVYEKIIGKTARKWLSLPFGKKHYPKPNLSLRPSFAV
                     NQFYPNVKEKWRKNTLAAIYTTPAISENYIYYGDDKGYFYCIFANNGNLKWKFKTGNR
                     IISSPTVQNGKVVFGSCDGNIYCLNTQNGNLIWKFETANAVLGCPYIQNDTVLMGGSD
                     GYFRALNLHTGNLIWQYEGIKNYIETRPVVANGKVYFGAWDTYFYALHLKDGSLAWKW
                     NNGNSNIFYSPAAVLPVYSYNKIFITAPDRFWTSLDAETGKVVWRTNQHEVRETIGIS
                     EDKNTVYSRAMNDSVFAVDVQTNYPKIIWKTNAQFGYDHNPNMLIERNGVIVFATKNG
                     LLVGINAKDGSVNWKHKIGNSIINTVSFISNNEFIITTTEGVIARIKF"
     gene            complement(3135..>3271)
                     /locus_tag="P5236_04260"
     CDS             complement(3135..>3271)
                     /locus_tag="P5236_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004342335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HRU63607.1"
                     /translation="PAMTLEEIGDELGLTRERVRQIKEKAIRRLRTSSKSKSLKSYLG
                     "
BASE COUNT         1074 a          563 c          511 g         1123 t
ORIGIN      
        1 gtaaaatatc agaaagaata agagttcgcg ataaatcttt ggaagctgct tgttggttta
       61 aaaaatcgag tgcaatggtc aaagaattga ggtctgaatt ataagtatca ttgataatca
      121 aacagttatg aattccttgt tttacttcca gccgcatttc tactggttct aattgcaaaa
      181 cgcgtttttt tattgttccc acatcatagt tcaaatataa catcaaactg atgcattgca
      241 aaccattttc aatggaagca tcatcaataa aaggaatcgt aaaactatct tcatgatttt
      301 gatattttaa actaatttct gttgtatttg attgtttttc aatgcttaaa accctgacaa
      361 cagctttttc atttcttccc caggaaaaca atttcccttt aaaattggat tgagcaatgg
      421 taatgtccac aagtttgtta tcacagcaat aaatcaagac ttctgtatga gtaaaaagtt
      481 tgagtttctc ttcacatttt tgtttcaagc tggaaaagtt ttcctgatga gcttcaccca
      541 aattagtaaa aatgccaatg gtaggtcgaa tcatcagttc taaacgttcc atctcgtgag
      601 gttgagaaat gccggcttca aatattccca attgtgtatt ttcattcaat gcccaaactg
      661 aaagcggaac gccaatctgt gaattataac tgcgagggga acgagtaatg tgataatcaa
      721 aacgaagcaa ttgatagagc cattctttta caacggtctt gccattgctt ccggtaatgc
      781 caatcacagg aatttgaaag gaatttcggt gaaaagctgc cagtcgttgc aaagcttcaa
      841 gagtgtcttt cacgaaaaga aaaattgttt tttcataact ttcaaattcc ggacgcattt
      901 cactcaccac aaaataatga acattttgtt cataaagttc accaatatat ttatgtccat
      961 catttctttg tgtcttgata gcaaaaaaaa gactttcaga tgggaaagaa actgttcggc
     1021 tatcagttaa caaccaatta atttcaacat ctggaatatt ctttttgact tttgcattaa
     1081 gaaccgttgc aatttgagaa agtttcattt tgttaaaatt ttatccttgc tattacgcct
     1141 tctgttgtgg taataataaa ttcgttattt gaaataaaag aaacagtatt gataatagaa
     1201 ttgccaattt tgtgtttcca gtttacagaa ccatcttttg cgtttattcc taccaacaaa
     1261 ccattttttg ttgcaaaaac aattactcca tttcgttcaa taagcatgtt gggattatga
     1321 tcatacccaa attgagcatt ggttttccag attattttcg gataatttgt ttgtacatcg
     1381 acagcaaaca cactgtcgtt catagctcga ctgtaaacgg tatttttatc ttcagaaatt
     1441 ccaatcgttt ctcgtacttc atgttgattg gttcgccaaa caacttttcc tgtttctgca
     1501 tccaatgatg tccagaaacg atctggtgca gtaataaata ttttattgta tgaataaact
     1561 ggcaaaactg ctgctggaga ataaaaaata tttgaattgc cgttattcca tttccaagct
     1621 aaactgccat ctttaagatg taatgcataa aaataggtat cccacgcacc aaaataaact
     1681 tttccattag caacgacggg acgagtttct atataatttt ttattccttc atattgccag
     1741 attaaattac ctgtatgtaa atttaaagct ctaaaatatc catcacttcc tcccataagt
     1801 acagtatcat tttgaatata cggacatccc agaacagcat ttgctgtttc aaatttccag
     1861 attaaatttc cattttgagt attcaaacaa tagatatttc catcgcatga gccaaaaact
     1921 actttcccat tttgaacagt aggcgaagaa ataattctgt taccagtttt aaatttccat
     1981 tttaaatttc cattatttgc aaaaatacag taaaaataac ctttatcatc accatagtaa
     2041 atataatttt cagaaattgc tggagtggtg taaattgcag ccagtgtgtt ttttctccat
     2101 ttttctttta cgtttggata aaattgattt acagcaaagg aaggacggag agataaattt
     2161 ggctttgggt aatgtttttt gccgaatggt aaacttaacc atttacgggc agttttacca
     2221 attatctttt cataaacata aatagaatca ctcacgttta aaattgaata gcctcctttt
     2281 ggctcatttc ctcttaaagt tgaacgatga atgatacctg gaatttcatc ataattcaaa
     2341 aaaacatttc gatgataatg tcctcccaat actgcttgaa cattatagcg acgaataaca
     2401 tcagttactt cataccaatt gtcaacatcg ccatttaaaa gtggataatg agttactaca
     2461 aatatgggat tatttttacc aattttcttt aattgttttt ttgtccattc aatatcttgt
     2521 ggtgctacgt gtccatcgcc cattttgata atgggtccag ttgcaaaacc aataaaataa
     2581 caatcgttat aagtaaaaga aaatttatca tcaccaaaaa ttttagcaaa atcggttgct
     2641 ccagattcac tccatttcat ttcatgattg cctggcacaa tgtaatatgg atttttgagt
     2701 tgatctaaaa ttcgtttagc catttcaagc gataatttat ctccataatt ggtaatatcg
     2761 cctgacacaa ggatgaattc aatctgatcg ttggtattga tttcatcaat taccatttga
     2821 aggtcttctg aagcttgagg attggttgca gaaatatgca aatcggttaa taatgcgaag
     2881 ctaaaaggac gttgagcgaa accaacgata aagaaacaaa taaaaaggta aacgactaaa
     2941 aaaattctct ttttcatctt ttatgcaaga agatttaata ataggttaca aagttacaat
     3001 tttcatttga gtattgacgg gtattttatg ggagaatttc acgaggcatt atctgaaaag
     3061 ggaagaaagt aaataataaa aaaaccgttc ataacttata tagaacggtt ttttatcatt
     3121 tgaataaaaa gaaattatcc taaataactt tttaatgatt tactttttga acttgttcgt
     3181 agtcgtcgga tagctttttc tttgatttga cgaactcgtt cgcgtgtcaa acctaactca
     3241 tcgcctattt cttcaagtgt cattgcagga a
//
LOCUS       DAOTKS010000173         3259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_24409_length_3259_cov_4.169788, whole genome shotgun sequence.
ACCESSION   DAOTKS010000173 DAOTKS010000000
VERSION     DAOTKS010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24409_length_3259_cov_4.169788"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="P5236_04265"
     CDS             <1..232
                     /locus_tag="P5236_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HRU63608.1"
                     /translation="KNLSYNKKDVFEITQPAKAGLPSPNVSSTYIFACNDKFFAYPNN
                     YNYYVSYYKNTFQHGGVSLEEMLIPFVMMLPK"
     gene            280..1029
                     /locus_tag="P5236_04270"
     CDS             280..1029
                     /locus_tag="P5236_04270"
                     /inference="COORDINATES: protein motif:HMM:NF013293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HRU63609.1"
                     /translation="MKIGLIGFGKTGKAVASVILQSPKFDLQWVLRRKKFLDDRNAAE
                     ILGVESDNQGLIHSIYSINIDELLDKQPVDAIIDFSSEEGIYIYGEAAAKRKIKIISA
                     ISHYKKETQDFLKSLSKKTTVFWSPNITLGVNYLLICAKFLKKIAPWVDIQIIEEHFK
                     GKKNVSGTAITIAEALDINKTEINSVRAGGIVGKHEVIFGFPYQTVRLIHESISREAF
                     GNGVLFVAENLENKETGLYNFEELLLPYFTA"
     gene            1057..2013
                     /locus_tag="P5236_04275"
     CDS             1057..2013
                     /locus_tag="P5236_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine carbamoyltransferase"
                     /protein_id="HRU63610.1"
                     /translation="MKKLMYVKDLGDLQEALQEAFEVKKNRFGFQELGKNKTLLMVFF
                     NSSLRTRLSTQKAGMNLGMNTIVLDINQGAWKLETERGVVMDGDKSEHILEAIPVMGC
                     YCDVIGVRAFAQFENKEYDYQETLINQFIQYSGRPVFSMEGATTHPLQAFADLITIEE
                     YKKRPHPKVVLTWAPHPKPLPQAVANSFADFMNEADVEFVITHPKGYELAPQFVRDAR
                     IEYNQMKAFEGADFIYAKNWSAYQDPNYGKILSKDLSWTVSSRQMAVTNNAYFMHCLP
                     VRRNMIVTDEVIESPQSLVIPEAANREISAQVVIKRMLNSLS"
     gene            2093..2617
                     /locus_tag="P5236_04280"
     CDS             2093..2617
                     /locus_tag="P5236_04280"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HRU63611.1"
                     /translation="MDLEQVIASIRNIPDFPKKGVQFKDITTAMKDADVLQFVSDELY
                     DYYKDKGITKVVGLESRGFVLGSILAYKLHAGFILIRKAGKLPAATYKVEYELEYGTD
                     SLEIHQDAMNENDIVLLHDDLLATGGSSSAALELIKKFHPKAIYVSFLIELKDLNGRE
                     KLKGVNDIHSLIQF"
     gene            2659..>3259
                     /gene="argB"
                     /locus_tag="P5236_04285"
     CDS             2659..>3259
                     /gene="argB"
                     /locus_tag="P5236_04285"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRU63612.1"
                     /translation="MKEELFIIKVGGKIIEDEVALQQLLIDFSKLEGNKVLVHGGGQL
                     ATTLATKLGIPTKMVDGRRITDDSMLKIITMVYAGWVNKQIVAKLQALDVNALGLTGA
                     DLNFISSEKRPIGEIDYGLVGDVKKVDVTLISNLIEKKVVPVVAPVTHDKTGNLLNTN
                     ADTIAAEVAKAFAEWFEVTLIFCFEKKGVLLNEEDEESVI"
BASE COUNT         1146 a          455 c          630 g         1028 t
ORIGIN      
        1 aaagaatttg agttataata aaaaagacgt ttttgaaatt actcagcctg ctaaagcagg
       61 tttacccagt cccaatgtaa gttctactta tatttttgct tgtaacgaca aattttttgc
      121 ttatcccaat aattataatt attatgttag ttattataaa aacaccttcc agcacggagg
      181 agtttcactg gaagaaatgt taataccatt tgttatgatg ttgccaaaat agagaaaggc
      241 aagtgattga ttatttgttt aatataaaaa attagaaaaa tgaaaattgg attaataggt
      301 tttgggaaaa cagggaaagc agtcgcttct gttattttac aaagcccaaa atttgactta
      361 caatgggttt tacgaagaaa aaaattttta gatgacagaa acgctgccga aattttgggc
      421 gttgaatcag acaatcaagg tttgatacat tctatttaca gcattaatat tgacgaactt
      481 cttgacaaac aacctgttga tgctattatt gatttttctt cagaagaagg aatttatatt
      541 tatggagaag cggctgcaaa aagaaaaata aaaattatct cggctatttc tcattataaa
      601 aaagaaaccc aagatttttt aaaatctcta tcaaaaaaaa cgactgtttt ttggtcacct
      661 aatatcacat taggagttaa ttatttatta atttgtgcta aatttttaaa gaaaattgct
      721 ccgtgggtag atattcaaat tattgaagaa cattttaaag ggaaaaaaaa tgtatcagga
      781 accgcaataa caatagcaga agcattagat atcaataaaa cagaaattaa ttctgttcgt
      841 gcaggtggca tcgttggtaa acatgaagtt atttttggat ttccttatca gactgttcga
      901 ttaatacatg aatcaatttc gagagaagct ttcggaaatg gggttttatt tgttgcagaa
      961 aacttggaaa ataaagaaac aggtttatat aattttgaag aattacttct tccttatttt
     1021 accgcataaa attcatttat ttgaaataat gtgtcaatga aaaaacttat gtatgttaaa
     1081 gatctcggag atttgcaaga agctctccag gaagcctttg aagtgaaaaa aaatcgtttt
     1141 ggatttcaag aattaggtaa aaacaaaaca ttactgatgg ttttttttaa ttccagtctg
     1201 cgaactcgac tgagtaccca aaaagcaggg atgaacttgg gaatgaacac catagtgctc
     1261 gatatcaatc aaggtgcatg gaaacttgaa acagaacgtg gtgttgtgat ggatggtgat
     1321 aaatcggaac atattttaga agcgattcct gtaatgggtt gttactgcga cgtgattggt
     1381 gtacgtgctt ttgctcaatt tgaaaacaaa gaatatgatt atcaagaaac gttaatcaat
     1441 cagtttattc aatattctgg tcgtcctgtt ttcagcatgg agggtgcaac tacacaccct
     1501 ttgcaagctt tcgccgattt aattaccatt gaagaatata aaaaaagacc tcatccaaaa
     1561 gtagttctta cgtgggcacc tcatccaaag ccgcttccac aagcagttgc aaattctttt
     1621 gctgatttta tgaatgaagc ggatgtagaa tttgtgatta cgcatcccaa aggttatgag
     1681 ctggctcctc aatttgttcg tgatgccaga atagaatata accagatgaa agcttttgaa
     1741 ggagcagact ttatttatgc taagaattgg tcagcttatc aagatcctaa ttatggtaaa
     1801 attttgtcca aagatttaag ctggacagta agtagtcgtc aaatggcagt aactaataat
     1861 gcttatttta tgcattgttt acccgtcaga agaaatatga ttgtgaccga tgaagtaatt
     1921 gaaagtccac aatcactggt gattcctgaa gctgcaaacc gagaaatttc ggctcaagtg
     1981 gttattaaaa gaatgttgaa ttcattaagt tgagcaagaa ttatttttac acttttataa
     2041 caaggttaaa tatatattta tctaaaaatt aaaacattaa gttaaaaaaa atatggattt
     2101 ggaacaagtt attgccagca tcagaaacat ccctgatttt cccaaaaaag gagtgcaatt
     2161 taaagatatt accacggcaa tgaaagatgc ggatgtttta cagtttgttt ctgacgaact
     2221 ttatgattat tacaaagaca aaggtattac gaaagtagtc ggtttggaaa gtcgtggttt
     2281 tgttttggga agcattctgg cttataaact tcatgcgggg ttcatattga ttagaaaagc
     2341 cggaaaactt ccagcagcaa cttataaagt ggaatacgaa ctggaatatg gtacagattc
     2401 acttgaaatt catcaagatg caatgaatga aaatgatatt gtacttttac atgatgattt
     2461 gttagctaca ggtggaagtt ccagtgcagc gttagaatta ataaaaaaat ttcatcctaa
     2521 ggcaatctat gtaagttttt tgattgaatt gaaagactta aacggtcggg aaaaattgaa
     2581 aggtgtaaat gatattcatt cccttattca attttaagcc aataaaattt ttcaactcat
     2641 ttcttaaagg taataaaagt gaaagaagag ctattcataa taaaagtagg tggcaaaatt
     2701 attgaagatg aagttgcgtt acagcaattg ttgattgatt tttcaaaact ggagggcaat
     2761 aaagtcttgg ttcatggtgg tgggcaattg gcaactacat tggctactaa acttggaata
     2821 ccaacaaaaa tggtcgatgg cagaagaatc actgatgatt ccatgctgaa aatcataaca
     2881 atggtttatg ctggctgggt aaacaaacaa atcgttgcta aattacaggc tcttgatgtg
     2941 aatgctttgg gtttaacagg tgcagatttg aattttatct cttcagaaaa acgtcccatt
     3001 ggagaaattg attatggttt agtgggcgac gtaaaaaaag ttgatgtaac gctaatcagc
     3061 aacttgattg aaaagaaagt cgttccagtg gtagctcctg taacacatga caaaacaggt
     3121 aacttactaa ataccaatgc cgataccatt gctgctgaag ttgcaaaagc ttttgctgaa
     3181 tggtttgaag taactttaat tttttgcttt gagaaaaaag gagttttgtt gaatgaagaa
     3241 gatgaagaaa gtgtcattg
//
LOCUS       DAOTKS010000174         3257 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_24438_length_3257_cov_2.886633, whole genome shotgun sequence.
ACCESSION   DAOTKS010000174 DAOTKS010000000
VERSION     DAOTKS010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24438_length_3257_cov_2.886633"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..2802)
                     /locus_tag="P5236_04290"
     CDS             complement(112..2802)
                     /locus_tag="P5236_04290"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRU63613.1"
                     /translation="MIIHFKIPYFTHWGQQIYVKGNIPELGNNETDKGLLLHFTFPEN
                     WIGEVEIVGNKPIEFSYKYFLYDENVNEWKEEWIEDRNIALDPSKSDHFFCFDFWNNP
                     GSIENVFYTAPFKNVFFRQNISLNLSSPQDYTHIFRIKVPLLQEGQVPCLIGDCKKLR
                     NWNIQQPLLLQKTDGNWWKVEVDLNEEKNEIHYKYGIYDTKNNRFLFFESGYDRKAPT
                     FHSNRTCIRLSDGFLRMNVQTWKGAGVSIPVFSIRTKKSFGVGDFLDLKLLVDWAKKI
                     GLKLIQVLPLNDTIGTHTDADILPYAAISAFALNPLFLNLLAIGELPENHPLQKEYKK
                     LQPALNDKEWIDYLTVINFKLKYAKELFIIQKEEFFKKPDYQTFFVQNKHWLIPYAAF
                     CVLRDQFETPDYTQWGDYAKYDAEKIANFTSPSQPHYQEIAYYYFLQYYLHKQLVEAT
                     QYAHEHGIVLKGDIPIGVNRYSVETWFHPEYFNMNMQAGAPPDLFSIKGQNWELPTYN
                     WEAIKQSKYEWWINRLYQMNYYFDAFRIDHILGFFRIWQIPLNQVEGIMGHLYPSIPI
                     YINEFNEKGIWFDYNRFCKPFINDEILGRFFGDDAGWVRASCLQIENGWNFQLKPEFQ
                     SQKAVQKMFEEGKFSEKIKCGLFDLISNVLFFEVEGSNGTQFYPRWGMHYLPSYLYLD
                     DYTKQKLDELYIDYFYRRQDDFWYYSGIKKLSVLKHATNMLICGEDLGTLTPCVTRAM
                     KELGILSLEIQRAPKTDKIEFLHPNDAPYLSVITPSTHDMSTIRGWWEENREVTQRFY
                     NYQLGHWGEAPYFCEPWICREILLQHLYSPAMWAIFQIQDLLSISEKLRRNNPHDERI
                     NVPTNSKQSWRYRFHLNLEDLLEEDDFNEELKNYIIQSGR"
BASE COUNT         1100 a          529 c          504 g         1124 t
ORIGIN      
        1 aggatctggt gccgaaaggc gtgggggttc gagtcccttc agccgcacaa aaaagtcttt
       61 taagtttttt atggcttgaa agactttgtt tttaaagtct gacattgatt tttatcgacc
      121 tgattgaata atataatttt ttaattcttc attaaaatca tcttcttcca gcaaatcttc
      181 aagatttaga tggaaacgat atcgccaact ttgtttagaa ttggtgggta catttattcg
      241 ttcatcgtgc ggattgtttc tacgtaattt ttctgagatg cttaacaaat cttgaatttg
      301 aaaaatagcc cacatagcgg gagaatacag atgttgcaaa agaatttctc tacaaatcca
      361 cggttcgcag aaataagggg cttctcccca gtgacctaat tggtaattgt aaaatcgctg
      421 ggtaacttca cgattttctt cccaccatcc gcgaatggta ctcatatcat gtgtagaagg
      481 cgtgataact gataaataag gagcatcatt tggatgaaga aattcaattt tatcagtttt
      541 tggtgcgcgt tgaatttcca agctaagaat accaagctct ttcattgctc tggtaacaca
      601 aggagtaagt gtacccaggt cttcaccaca aattagcatg ttggtagcat gtttaagtac
      661 acttaatttt tttattccag aataatacca aaaatcgtcc tgtcgtcgat agaaataatc
      721 aatatataat tcatccagtt tttgtttggt ataatcatca agatatagat aagaaggcag
      781 atagtgcatt ccccagcgag gatagaattg tgtaccatta gaaccttcca cttcaaaaaa
      841 caaaacattt gaaattaaat cgaacaagcc acatttaatt ttttcagaaa attttccttc
      901 ttcaaacatt ttctgaaccg ctttttggct ttgaaattca ggctttaact gaaaattcca
      961 accattttct atttgtaggc aacttgctct tacccaacct gcatcatcac caaaaaatct
     1021 tcctaaaatt tcatcattga taaaaggttt acaaaaacgg ttgtagtcaa accaaatgcc
     1081 tttttcgtta aattcattga tgtaaatggg aatggaagga tacaaatgtc ccataattcc
     1141 ttctacctga ttaagaggaa tttgccaaat acggaaaaaa ccaagaatat gatcaatacg
     1201 aaaggcatca aaataataat tcatttgata aagacggtta atccaccatt catattttga
     1261 ttgtttgatg gcttcccaat tataggttgg caattcccaa ttttgacctt tgattgaaaa
     1321 caagtctggt ggagcgccag cttgcatgtt catattgaaa tattcagggt gaaaccaagt
     1381 ttctacgctg taacggttta caccaattgg gatatctcct ttcaaaacaa tgccatgctc
     1441 gtgtgcatat tgagtggctt caaccaactg tttatgcaaa taatattgta aaaaataata
     1501 ataagcaatt tcttgataat gaggttgaga tggagaagta aaattagcaa tcttttcagc
     1561 atcatatttt gcataatctc cccattgtgt ataatcaggt gtttcaaatt gatcacgaag
     1621 tacgcaaaaa gctgcatatg gaatcaacca atgtttattt tgaacaaaaa atgtttgata
     1681 atcaggtttt ttaaaaaatt cttctttttg tataataaaa agttcttttg catattttaa
     1741 tttaaaattt atcactgtca gataatcaat ccattctttg tcatttaaag caggttgtag
     1801 tttcttatat tctttttgca aaggatgatt ttcgggaagt tcgccaatgg ctaataaatt
     1861 taaaaagagt ggattaagag caaaagctga gatagctgca tagggcaaaa tatctgcatc
     1921 ggtatgagtg ccaattgtat cattaagtgg caggacctga attaatttca atccaatttt
     1981 tttagcccaa tccaccagca gttttaaatc cagaaaatca cccacaccaa aacttttttt
     2041 agttctgata ctaaaaaccg gaatagaaac accggctcct ttccatgttt gaacattcat
     2101 tcttaaaaaa ccatctgaaa gtcgaataca tgttctgttt gagtgaaagg taggagcttt
     2161 tcgatcgtaa cctgattcaa aaaatagaaa acgattgttt ttggtatcgt aaattccata
     2221 tttataatgg atttcattct tttcctcatt taaatctact tcgactttcc accaatttcc
     2281 gtcagttttt tgtaacaata atggttgttg gatattccaa tttcgtaatt tcttacagtc
     2341 tcctatcaaa cacggtactt gaccttcttg tagcaaagga acttttattc taaaaatatg
     2401 ggtataatct tgtggagaag aaagattaag agaaatattt tgtcgaaaaa acacattttt
     2461 aaaaggtgct gtataaaata cattttctat actacctgga ttattccaaa agtcaaaaca
     2521 aaaaaaatga tctgatttgg aaggatcaag ggcaatgttt cgatcttcta tccattcttc
     2581 tttccattcg ttaacgtttt cgtcatataa aaaatactta taagaaaact caatgggttt
     2641 atttccaact atttctactt ctcctatcca attttcggga aaagtgaaat gtaataaaag
     2701 ccctttatct gtttcattgt tccctaattc agggatattt ccttttacat aaatttgttg
     2761 cccccaatga gtaaaatagg gtattttaaa atgtatgatc atatgatttt gttatgttgc
     2821 attgaactct ttcttcaaaa ttaaattaat tctattatca caataaattg tttttcaaaa
     2881 atttaaataa caaagagcga gaaaatattt tatcaaaaat acatttaatt tttaccaagg
     2941 agtattgaaa ctttaaaaaa aatcataaaa attagttcaa acgtttgcat aaaacaattt
     3001 gaacagcaga gttgagcatt tgaaatttaa tacccctata cagaagttga tattatgttg
     3061 attcccagtt ttgtttaaat tagaatcaat aaattatgag ataatcgaaa cctttgaaac
     3121 attttaatga gagtttaaac caacttttta atggcaaaga agtattttgg aaataatttt
     3181 gttccaatag agaatttttt attttaattt ctgtaatttt gtaaaaaatt cttttatcta
     3241 cataaaatta tggtaat
//
LOCUS       DAOTKS010000175         3253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_24471_length_3253_cov_6.914321, whole genome shotgun sequence.
ACCESSION   DAOTKS010000175 DAOTKS010000000
VERSION     DAOTKS010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24471_length_3253_cov_6.914321"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="P5236_04295"
     CDS             <1..344
                     /locus_tag="P5236_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63614.1"
                     /translation="FLDKIIEIYNFKTQKNMKAEMVDICKAVGNRIGAYCRENDDKGI
                     LFSIRNAKSRTEFLNVLAETQFRTGVSYSETFFKELPDNSQWEEYKALVSIFAMNSFL
                     FKEGKTTENNK"
     gene            363..1304
                     /gene="cas7i"
                     /locus_tag="P5236_04300"
     CDS             363..1304
                     /gene="cas7i"
                     /locus_tag="P5236_04300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HRU63615.1"
                     /translation="METNQTVNCVNIAYIFKTSLGSINGSWTEGNVSTVKKITLPNGT
                     QLPYVSGQSIKYQIRKAWKEMGLGDLLSEVKQAEKKAGVDFTSGEPNLYLDDDLLGYM
                     IASSGENRRRTAVVRTSAAIGLFPFRNDRDLGTKSKEQTGGDVSAGGNIFETEIYYNY
                     FRVNILVELDRLGVFQDFELAKENKGKVQSLAPQEKRERLIYFMRALSHIWGGGKQSR
                     LLTDMSPKFLAITFQNTKSPIFLETLTVSQNEELNTKSINEVLKGNSGIIKKQFLGFQ
                     SGIFKNDDEISLLPEIMNIQSTFDNTIEYINNELFQQ"
     gene            1334..2056
                     /gene="cas5"
                     /locus_tag="P5236_04305"
     CDS             1334..2056
                     /gene="cas5"
                     /locus_tag="P5236_04305"
                     /inference="COORDINATES: protein motif:HMM:NF021240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HRU63616.1"
                     /translation="MNSFRQPDFHTYHKTLPLPPKTTVAGMIGSALGISPEKVNDEWL
                     KNNRFQMGIVGKNNGKASDLWQIRKYTDRYIKDYIKGKNQYPYFSAVIVRELLYSSEF
                     ILYLHFEDENDYELVSSKFRNPEWALSLGREDELVLIKEIRQINLEEKSNISFCNTVL
                     PIDFTKTPYEIVLNMQNTSGNLLDEAPKSIKLPITFSYNDDGARESNEYQQFSFIFNL
                     PIKPKDSRGFYDEELNYSFQIF"
     gene            2060..>3253
                     /locus_tag="P5236_04310"
     CDS             2060..>3253
                     /locus_tag="P5236_04310"
                     /inference="COORDINATES: protein motif:HMM:NF037707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HRU63617.1"
                     /translation="MKYTEILAKSEPQKTLIEHTNDSLYWFSKVLNWYEVLISKMSEY
                     YSLSKPNIIKRLFITVAFHDIGKATERFQDKVRGLPLNMLESHALTSVPFIYNEIKEN
                     PIKVIDGNPFFPEILAIASHHSKLKNGLFQDYERMRITFADKDYFQTFYENINQQAIK
                     LNIPNWQFLKFYDEVIEENPYWIFYDEVMNYIGSYKYEKTNVIRDLFLLFKSVLHYCD
                     WLASSNNTTYNYSATENNESITAKMKIKVPQFVGWESFQLHAAKSSSKNVFIQIPTGQ
                     GKTEASILWATQNNVNQKILFLLPTMVTTTKMWERMTYLFGGTENVGLSHSTAKYILR
                     EKEEDMEPETLRSHYLYNRTFFKPVTVATLDQLIYSFFNWGYWVLTGAASFNAKIIID
                     EVHIYD"
BASE COUNT         1187 a          484 c          584 g          998 t
ORIGIN      
        1 tattcctgga taaaatcatt gaaatatata attttaaaac ccaaaaaaat atgaaagcag
       61 aaatggtaga tatttgtaaa gcggttggaa accgtatcgg cgcctattgt cgtgaaaatg
      121 atgacaaagg gattcttttt tcaatacgaa atgccaaaag caggactgag tttcttaacg
      181 tattggctga aactcagttt cgaaccggag tatcttatag cgaaactttt ttcaaggagc
      241 tacctgataa ttcccaatgg gaagaatata aggcattggt aagcattttt gccatgaata
      301 gcttcttatt taaggaaggt aaaacaacgg aaaataataa ataatattaa aaaaaattaa
      361 ttatggaaac aaatcaaacc gttaattgcg taaatatcgc ttatattttt aaaacttcac
      421 taggaagtat aaatggcagt tggacagaag gtaatgtcag tactgtaaag aaaatcactt
      481 tacctaatgg tacacagcta ccatatgttt caggacaaag tattaaatat caaattcgta
      541 aggcatggaa agaaatggga ttgggagatt tactgtctga agtaaaacag gctgaaaaaa
      601 aagccggtgt tgacttcact tccggagagc caaatctata tttagacgat gatttattgg
      661 gttatatgat tgcctcctct ggagaaaata gacgccgcac agcagttgta cgtacttctg
      721 ctgctattgg attattccca tttaggaatg atcgtgattt aggcacaaaa tcaaaagaac
      781 aaacaggagg tgatgtgtca gctggcggaa atatttttga aacagagatt tattacaact
      841 atttcagagt aaatattttg gtggagcttg atagattggg cgtatttcag gattttgaac
      901 tggcaaaaga aaataaaggc aaagttcaat cattagcacc tcaagaaaaa cgagaaagat
      961 tgatttattt tatgagagca ctttctcata tttggggagg aggcaaacag tcacggttgc
     1021 tgactgatat gtcaccaaaa tttttggcta ttacttttca aaacactaaa tcacccattt
     1081 tccttgaaac attgaccgtt tcacaaaatg aagagttaaa cacaaaaagc ataaatgaag
     1141 tacttaaagg aaattctgga attattaaaa aacaattctt aggtttccaa tctgggattt
     1201 tcaaaaatga tgatgagatt tcacttttac ctgagataat gaatatacaa tcgacatttg
     1261 ataatacaat agaatatata aacaatgaat tgtttcagca atgaagttta taagagtaat
     1321 atgtttcggt agcatgaatt catttcggca accggacttc cacacctatc ataagacact
     1381 ccctcttcct cccaaaacga cagtagcggg aatgattggt agtgctttgg ggatttctcc
     1441 tgaaaaagtt aatgacgaat ggttaaaaaa taaccgattt cagatgggaa ttgtgggaaa
     1501 aaataatggc aaagcaagtg atttatggca aataagaaaa tatacagata gatacatcaa
     1561 agattatatt aaaggtaaaa atcaatatcc atacttttct gccgtaattg ttagagaatt
     1621 attgtattca tcagaattta ttctatatct tcatttcgaa gatgaaaatg attatgaatt
     1681 agtcagtagt aaattccgga atcctgaatg ggcactttct ttgggtaggg aggatgaatt
     1741 agtgttgatt aaagaaattc ggcaaataaa tttggaagaa aagtcaaata ttagtttttg
     1801 caataccgtt ttaccaattg attttacaaa gacgccttat gaaattgtgt taaatatgca
     1861 aaatacttca ggtaatttat tagatgaagc acccaaatcg ataaaattac ctataacatt
     1921 ttcatacaat gatgatggag caagagaatc aaatgaatat cagcaatttt catttatctt
     1981 taatttgcct atcaaaccca aggatagcag aggcttttat gacgaagaac taaactattc
     2041 attccagatt ttttagttca tgaaatacac tgagatttta gcgaaaagcg aaccccaaaa
     2101 aacattgata gagcatacga acgatagctt atattggttt tcgaaagttt tgaattggta
     2161 tgaagttctg ataagtaaaa tgtcggaata ttattccctt tccaagccca atataattaa
     2221 acgtcttttt attacagtgg ctttccatga cataggtaaa gccacagagc ggtttcagga
     2281 taaagttagg gggcttcctt tgaatatgct tgaatctcac gcgttaactt ccgtcccttt
     2341 tatttacaat gaaataaaag aaaaccctat aaaagttatt gacggaaatc cattctttcc
     2401 ggaaattctc gctatagcaa gccatcacag caaattgaaa aatggactgt ttcaagatta
     2461 tgaaagaatg agaattacct ttgccgataa agattatttc caaacattct atgaaaacat
     2521 taaccagcag gcaataaaac taaatatccc caattggcaa tttttaaaat tttatgatga
     2581 agtaattgaa gaaaatcctt attggatttt ctatgatgag gtaatgaatt atataggttc
     2641 ttataaatat gaaaagacaa atgtaatcag ggatttattt ttacttttta aatcagtttt
     2701 gcattattgt gattggttag cttcctccaa taatacaacc tataattatt ctgctactga
     2761 aaataatgaa tcgattacag ctaaaatgaa aattaaagtg cctcaatttg ttggttggga
     2821 atcttttcaa ttgcatgccg caaaatcatc ttcgaaaaac gtttttattc agatccccac
     2881 aggacaggga aaaacggaag cttctatttt atgggcaaca caaaataatg tcaatcagaa
     2941 aatcttattt ttattaccta caatggtaac aacaaccaaa atgtgggaaa gaatgactta
     3001 cttgtttgga ggcacagaaa acgttggcct ttcacatagt acggcaaaat atatattaag
     3061 agaaaaagaa gaagatatgg agccggaaac attgagaagt cattatctat ataatcgcac
     3121 gtttttcaaa cctgtgactg tcgctaccct tgatcaacta atatatagct tttttaattg
     3181 gggatattgg gtattgaccg gagcagcaag ttttaatgca aaaattatta ttgatgaagt
     3241 tcatatttac gat
//
LOCUS       DAOTKS010000176         3243 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_24569_length_3243_cov_3.274467, whole genome shotgun sequence.
ACCESSION   DAOTKS010000176 DAOTKS010000000
VERSION     DAOTKS010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24569_length_3243_cov_3.274467"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /locus_tag="P5236_04315"
     CDS             <1..1058
                     /locus_tag="P5236_04315"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRU63618.1"
                     /translation="TLTLIQGASILPGDKISYTNGTIEWKTSQNSNAYGTYTFTDYVY
                     GTDCSSLPTVTTDPSSISFSPTQGIQTFTVIGTNLTSAITITPPRGLTVNPSTISPDE
                     NGEINEVVTVNWTGGTTSGSTIQISGGGLVSNVAVLVNTSGFSAYCNKVIYQQNSTNY
                     PAYMTIDMSSDKTQMVFTIAPYNEGDGTIWNRIVNIVVNGGTANELVASNVLSADKTQ
                     IIITFTQPLQDGDEIMFGAPMVWETNANHNIFIDAYQGPYIVGQSCDLEDQGPYVSVS
                     KVLDEIINIYPNPAKDVLNVTPSVYKTEFYSLEGQLLYSVVNVYEIPLSTFNKGMYVV
                     RLTDKAGNVVAKKLVIQ"
     gene            1422..>3243
                     /locus_tag="P5236_04320"
     CDS             1422..>3243
                     /locus_tag="P5236_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63619.1"
                     /translation="MKKGIFLFFVLSFSLRSFSQNPVQVGAGSYAEYPPISVWDEDGY
                     FAKTYRWFHDNWSNLYIHENVRQKPLPTNKWWTNYVFSQYGGDAWAYPHAVSADKEGI
                     NIKIPSGFQGGGMITTPFLEIKGASHLAANEEKIVFADFESTTYPEGWTVGPNPPYPG
                     PVSLNDISQTPTPTGFTGNRFIDSYKGNEAKLTLISPSFVITKNYIHLRVGGGNYIND
                     TYVGLFINGVRVFAETGQNSGTLTQRTWDVSNYIGQTAEIRIVDNSTGGWGFIMCDEI
                     IFSDSNFTGSGYENDFSPKNSNVYDWTDLGFTFRSEDQDGRYMDVTLVHGIPFTWLEL
                     NNLVPLLIPGATCRIYNLSGEEVTNFPATLNAFTMEFGNRIYGVHLPSGSVLYRSSGG
                     DFQVEIPTSSPKYLVISDLPNRNLLSLYDAYARNKPVNTSFLWDYKISEGKINTTFQI
                     DTKNLETGALNGETLMSFLPHHYRNTTTNFNFISGGDYQLILGKMHTASGRVFSIDYP
                     FGGMPPYLPAPLNLTATQKNRLNTMLNYKADHSGGFNGNTYAKGLGENSNVMLMAKTM
                     NANSAFTTLKNNLKTELTDWWTYHPSEASNKKYFFANYPDY"
BASE COUNT         1026 a          511 c          624 g         1082 t
ORIGIN      
        1 atactttaac acttatacag ggagcttcaa ttttgccagg agataaaatt tcttatacaa
       61 atggtacaat agagtggaaa acatcacaaa attcaaatgc ttatggtact tatacattta
      121 cagattacgt ctatggcact gattgttcat cattacctac ggttactact gatccttcca
      181 gtatttcgtt cagtccaacc caaggaatcc aaacatttac tgtaattgga acaaatttga
      241 cgagcgcaat taccattact cctccaagag gattaacagt aaatccatcg accatatcgc
      301 cagatgagaa tggagagatt aacgaggtag tcacagttaa ctggactggc ggaacaactt
      361 caggttcaac tatccagatt tctggtggtg ggcttgtttc aaatgtagct gtattagtaa
      421 atacaagtgg cttttcagcc tactgcaata aagttattta tcagcaaaat agtactaact
      481 atcctgctta catgactatt gatatgagta gtgataaaac tcaaatggtg tttaccattg
      541 caccttacaa tgaaggtgat ggtacaatat ggaatagaat tgttaatata gtagttaatg
      601 gagggactgc caatgaatta gttgcttcta atgttctttc agctgataag acgcaaatca
      661 ttattacttt tacgcaacct ttgcaagatg gtgatgaaat tatgtttggt gctcctatgg
      721 tttgggaaac caatgcgaac cataatattt tcattgatgc ttaccaaggt ccttatatag
      781 taggtcaaag ttgtgactta gaagatcagg gaccttatgt ctctgtatcc aaagttttgg
      841 atgaaataat taatatttat ccaaatccag caaaggatgt tctcaatgtt acgccatcgg
      901 tttataaaac agagttttat tctttggaag gacaattgct ttattcagta gtaaatgtat
      961 atgaaattcc tctatcaact ttcaataaag gaatgtatgt agtacgcctc acagataagg
     1021 cagggaatgt ggttgctaaa aaattggtga ttcagtaatt ttttttaaca gaagagggtg
     1081 tttaaaacac cctcttctgt taactcttct tcaatcaggg cattagtttg gaaaatttga
     1141 tgatgtgagg gggtgctttt ctgtgtctca accacacaaa taataaatga tacggtagct
     1201 ttgaaagttt gagtgaagag tttatgtgtt tgacagcaaa aaagaaaaaa ttttgttgca
     1261 aagaatttgt aattctttgc gggtttgata gcggatttaa aattcgtaaa taaccaacta
     1321 tcggattgta aatccgatag aacggataga aaatattaaa aatcaccctt ttatttgttc
     1381 cttccgttag aatattttta accttaaaat aaatattttc catgaaaaaa ggcattttct
     1441 tattttttgt tctttccttt tctttaagat cgttctcaca aaatccagtt caggtaggag
     1501 ctggcagtta tgctgaatat cctcctattt cggtttggga cgaagatggt tattttgcaa
     1561 aaacttatcg atggtttcat gacaactgga gcaatttgta tattcatgaa aatgttcgtc
     1621 aaaaaccgtt accaaccaat aaatggtgga caaattatgt tttttcacag tatggtggcg
     1681 acgcttgggc ttatcctcat gcagtatcag cagataagga agggattaat ataaaaattc
     1741 ccagtggttt tcaaggcggt ggtatgatta ctactccttt tctcgaaata aaaggagctt
     1801 ctcatcttgc tgccaatgag gagaaaatag tttttgccga ttttgaaagt accacttatc
     1861 ctgagggatg gacagtagga cctaatcctc cttatcctgg acctgttagt ttgaatgaca
     1921 tttctcaaac acctacacct actggtttta caggtaatcg ttttattgat tcatacaaag
     1981 gaaatgaagc taaattaact ttgatttccc ccagttttgt tattacaaaa aactatattc
     2041 acttgcgtgt tggaggcggg aattatatta atgatactta tgtgggttta tttattaatg
     2101 gagtacgtgt gtttgcagag actggacaaa atagcggaac cttaacccaa aggacatggg
     2161 atgtttcgaa ttatattgga caaacagcag aaattcgcat cgtggataat tcaactggtg
     2221 gttggggatt tattatgtgt gatgaaatta tattttcaga tagtaatttt actggcagtg
     2281 gctatgagaa tgatttttcg cccaaaaatt caaatgttta tgattggaca gatttgggtt
     2341 tcactttcag aagcgaagac caggatggtc gctatatgga tgtaacatta gttcatggta
     2401 ttccttttac atggcttgaa ttgaataatt tagttccttt attaattccg ggtgcaactt
     2461 gtagaatata taatctttca ggagaggaag taacaaattt tcctgcaact ttaaatgctt
     2521 ttacaatgga gtttgggaat cgaatttatg gtgtccatct tccttcagga agtgttttat
     2581 atcgatcaag tggcggagat tttcaagtgg aaattccaac ttcaagtcca aaatatcttg
     2641 ttatttctga tttacctaat cgtaatttac tttctcttta tgatgcttat gccagaaata
     2701 aaccggtaaa tacttctttt ttatgggatt ataaaatttc tgaggggaaa atcaatacaa
     2761 cttttcaaat agatactaaa aatctggaaa caggggcttt gaatggcgaa acgttgatga
     2821 gttttttgcc tcatcattat agaaatacta ccaccaattt taactttatt agtgggggtg
     2881 attaccaatt aattttgggg aaaatgcata cagcttcagg tagggtcttt tcgattgatt
     2941 atccttttgg tggaatgcca ccttacttac ctgcaccctt aaatttaact gctactcaga
     3001 agaacaggct gaatactatg ttgaattata aggcagatca ttcaggtgga ttcaatggaa
     3061 atacttatgc aaaaggattg ggagaaaatt caaatgtaat gttgatggcg aaaactatga
     3121 atgcaaattc tgcttttaca acgttgaaaa acaatcttaa aacagaatta actgattggt
     3181 ggacatatca tccatcagag gcgagcaata aaaaatactt ttttgccaat tatcctgatt
     3241 atg
//
LOCUS       DAOTKS010000177         3230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_24671_length_3230_cov_4.540787, whole genome shotgun sequence.
ACCESSION   DAOTKS010000177 DAOTKS010000000
VERSION     DAOTKS010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24671_length_3230_cov_4.540787"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..783
                     /locus_tag="P5236_04325"
     CDS             157..783
                     /locus_tag="P5236_04325"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRU63620.1"
                     /translation="MKKLYLLILLIIPVIVFSQSRLDQPEIYLGTSHGVSASMIHFSP
                     SVEQEILLGYNGGIVFRYIDEKNVGIQAELNFFQRGWKEKGNIYAKQLNYIELPFLTH
                     IYFGNSSRFFINLGPKISYLLSENVLKNLSTNSTETQHITKVQHPFDYGICGGFGVLF
                     RVKKNIFQFDTRFNYSLSDIFSNSKKDYFETSNNINLSINLGWLLQIK"
     gene            975..1817
                     /gene="kduI"
                     /locus_tag="P5236_04330"
     CDS             975..1817
                     /gene="kduI"
                     /locus_tag="P5236_04330"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018665715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HRU63621.1"
                     /translation="MKTNYEIRYASHPQDVKNYDTSRLRKEFLVEKIFSKDEVNMVYS
                     LFDRIIIGGAMPVNEILPLETIEPLKSEYFLSRREFGTFNVGGPGKISVDGNVFELNY
                     KEALYLGAGNRKVYFESLDPSKPAKFYFNSTLAHRNYPDKKVTKANAVIAEMGSMESS
                     NHRNINKMLVSQVLDSCQLQMGMTELAPGSVWNTMPVHTHSRRMEAYFYFEVPEDEVV
                     CHFMGEPNETRHIWMKNEQAVISPQWSIHSAAATSNYTFIWGMAGENLDYGDQDFYKN
                     TDLR"
     gene            1922..2701
                     /locus_tag="P5236_04335"
     CDS             1922..2701
                     /locus_tag="P5236_04335"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HRU63622.1"
                     /translation="MNQFDLTGKIALITGASYGIGYAIAKAFYAAGATIVFNDINEEK
                     VNNGLTSYQKDGIKAHGYVFDVTNEKAVNEAIEKITKEVGVIDILVNNAGIIKRIPAL
                     EMSAADFKQVVDVDLNGPFIVSKAVAPGMIAKGGGKIINICSMMSELGRETVSAYAAA
                     KGGLKMLTKNLACEWGQYNIQVNGIGPGYITTPQTAPLRVDGHPFNNFIISRTPAARW
                     GTPEDLQGPAVFLASSASNFVNGHILYVDGGILAYLGKQPS"
     gene            2758..>3230
                     /locus_tag="P5236_04340"
     CDS             2758..>3230
                     /locus_tag="P5236_04340"
                     /inference="COORDINATES: protein motif:HMM:NF027479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HRU63623.1"
                     /translation="MKKNLFYILISLILASCSSLPKSIEITNPTSFDRKNEMVEIKNQ
                     EILKHFSRSSSYILKNEQNEEVPYQIIIDDNKKTEAFIFQANVKANQKTVYHFVKGKP
                     SPVKPKTFARFVPERKDDFAWENDLAAYRMYGPALADENPSNGVDLWLKCTDELVV"
BASE COUNT         1141 a          512 c          537 g         1040 t
ORIGIN      
        1 actttacatc tttatggtaa cagtttaaat actcaattaa attccattaa ttattccgaa
       61 ggattacaat ctgattttaa ttttcaacgt aactccaaga aatcaggctt tatcaatgtt
      121 caactttatt ggggcgaaag gaatttcaag taatttatga aaaaacttta tcttttaatt
      181 ttactaatta tcccagtaat tgttttttcg caaagtcgat tagaccaacc tgaaatatac
      241 ctggggacaa gtcatggagt ctcagcttcc atgatacatt tttctccttc tgtagaacag
      301 gaaattttat taggctacaa tggaggaatt gttttcagat acattgatga aaaaaacgtt
      361 ggaatacaag cagagttaaa ctttttccaa cggggatgga aagaaaaagg aaatatttac
      421 gccaaacaat taaattacat tgaacttcct tttttgactc atatctattt tggaaacagc
      481 agtcgttttt ttattaattt aggtcctaaa ataagttatt tgttatctga aaatgtatta
      541 aaaaatcttt caacaaattc aactgaaacg cagcacatta cgaaagtaca gcatcctttt
      601 gattatggca tttgtggggg cttcggcgtg ctttttagag tgaaaaaaaa cattttccaa
      661 tttgatacac gattcaatta cagtttaagc gacatatttt caaacagtaa aaaagattac
      721 tttgaaactt caaacaatat caatctttct ataaatctcg ggtggctgct acaaataaaa
      781 tagtttctta atcaaaaatt aagaaaaaaa acagtttttc cacctttttt gaacaaaatt
      841 acacttttta cctccctttt tttattaact ttgcttgaaa agaaattttg tcttcttttc
      901 ttgaatcaat tcaggtcaac attaatttta tttctaacct ttcgttttat caaaaaattt
      961 aaaatattac tcatatgaaa acaaattatg aaataaggta cgcctcccac ccgcaagatg
     1021 taaaaaatta tgacacttcc agattgagga aagaattcct cgtagaaaag attttttcaa
     1081 aagatgaagt gaatatggtc tattcattgt ttgaccgcat cattatagga ggcgccatgc
     1141 cagtgaatga aattcttcct ctggaaacca ttgaaccgtt aaagtcggaa tattttcttt
     1201 cgagacgtga attcggaacg tttaatgtag gaggacctgg caaaatttcc gttgatggaa
     1261 atgtttttga attaaactat aaagaagcac tttatttagg tgcaggaaat aggaaagttt
     1321 attttgaaag tttagaccct tcaaaaccag caaaattcta tttcaactct acccttgcac
     1381 atagaaacta tcctgacaaa aaagttacta aagcaaatgc tgtaatagct gaaatgggtt
     1441 ccatggaaag ttcgaatcat aggaacatta ataaaatgct tgtatcacaa gtacttgatt
     1501 cttgccagtt acaaatggga atgacagaac ttgctccagg aagcgtatgg aatactatgc
     1561 cagttcatac ccatagcaga cgaatggaag catattttta ttttgaagta cctgaagatg
     1621 aagttgtctg ccattttatg ggtgagccca atgaaacccg tcacatctgg atgaaaaatg
     1681 aacaagctgt tatttcacca caatggtcaa ttcattcagc ggctgctaca agcaactaca
     1741 cttttatttg gggaatggca ggtgaaaatc tggactatgg agatcaggat ttttacaaaa
     1801 atacggatct tcgataattt tctggaaata aacttttttg tttttttaaa tttcaatttt
     1861 tatcaatgaa gaacaaaatc ttgtaaaata tttaaactta atctttaaaa aaattaaaaa
     1921 aatgaatcaa tttgatttaa cagggaaaat tgcactaatt actggtgctt cttatggaat
     1981 aggctatgcc attgcaaaag ctttttacgc tgcaggagct actattgtat tcaatgatat
     2041 taatgaagaa aaagttaaca atggcttaac ttcctatcag aaagatggaa tcaaagcaca
     2101 tggctatgta tttgatgtaa ctaatgaaaa agctgtaaat gaagccattg aaaaaattac
     2161 caaagaagtc ggtgttattg atattttggt aaacaatgct ggtattatca aacgtattcc
     2221 agctcttgaa atgagtgctg ccgattttaa acaagttgta gatgtagatt taaacggacc
     2281 ttttatcgtt tcaaaagccg ttgctccagg aatgattgcc aaaggaggag gaaaaattat
     2341 taatatttgc tctatgatga gcgaattagg aagggaaaca gtttctgctt atgcggctgc
     2401 taaaggaggt ctaaaaatgt taactaaaaa tcttgcttgc gaatggggtc aatacaacat
     2461 tcaagtaaac ggaataggac caggttatat tactactcca cagacagccc ctttgcgagt
     2521 agatggacat cctttcaata attttattat atcacgtact ccagcggcac gttggggaac
     2581 accagaagat ttgcaaggac cagcagtatt tcttgcttcg agtgcttcca attttgttaa
     2641 tggacacatc ttatacgtag atggtggtat attggcatat cttggtaaac aaccttcata
     2701 ataaattaaa atatagccaa ttaattaaaa aattaattga cttttaaaaa tttgaatatg
     2761 aaaaaaaatc ttttttatat tttaatttcg ttgattcttg cttcgtgttc ttctctacca
     2821 aaatcaatag aaataactaa tccaacatca ttcgacagaa agaatgaaat ggtcgaaatc
     2881 aaaaatcaag aaattttaaa acattttagc cgatcttctt cttatatact taaaaatgaa
     2941 caaaatgaag aagtgccata tcaaattatt atcgacgata ataagaaaac agaagccttt
     3001 atttttcaag caaacgtaaa agctaatcaa aaaactgttt atcattttgt taagggtaaa
     3061 ccttctcctg tgaaaccaaa aacatttgct cgttttgttc cagaacgaaa agatgatttt
     3121 gcttgggaaa acgatttagc cgcttataga atgtatggtc cagctttagc cgacgaaaat
     3181 ccaagtaacg gcgttgacct ttggctaaaa tgcactgatg aattggtagt
//
LOCUS       DAOTKS010000178         3190 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_25044_length_3190_cov_5.280702, whole genome shotgun sequence.
ACCESSION   DAOTKS010000178 DAOTKS010000000
VERSION     DAOTKS010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25044_length_3190_cov_5.280702"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="P5236_04345"
     CDS             complement(<1..502)
                     /locus_tag="P5236_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRU63624.1"
                     /translation="MKKLVRLIFKYFGWQMIYPLPEPQKSVICLAPHTSNWDFLIGEL
                     SYWSIGRKASFLIKKSWFIFPLNFFFKSLGGIPIDRSKHNSLTQQMIDKFQKLDSFHL
                     VITPEGTRGCVTAWKKGFYYIALGANVPIQLSYIDYKKKELGIAKIFYPTGNIDADFQ
                     EIQNFYE"
     gene            760..3075
                     /locus_tag="P5236_04350"
     CDS             760..3075
                     /locus_tag="P5236_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRU63625.1"
                     /translation="MIEREKDTNFDFLNELNESQRLAVEYTDGPSLVIAGAGSGKTRV
                     LTYKVAYLLKKGFPPSSILALTFTNKAAREMKERIAEIVGNKIARYLWMGTFHSIFSR
                     ILRAEAQYTGFTQNFSIYDQSDSKSLLRSIIKEMELDSKIYTPGLVQSRISMAKNNLI
                     TPKAYAADYSLIEKDRKNHMGKLCDVYQTYCIRCKQANAMDFDDLLLQTNLLFRDHPE
                     VLQKYQQLFRYILVDEYQDTNFAQYLIIKKLAERHKNICVVGDDAQSIYSFRGANIDN
                     IFQFKNSYQDAKIFKLEQNYRSTKTIVKAANSLIKKNKEQIQKTIFSEKEPGAPIKVF
                     STYTDLEEGIVVANCVANLYSENFQYQDIAILYRTNSQSRVLEEALRNRNIPYRIYGG
                     LSFYERKEIKDVIAYCRLICNQQDEEALKRIINYPTRGIGETTLNKIIECAQQNQISL
                     WEVISNLAEYKLPINSRTVTKIEQFKELINVFVGQVNTQNAYDLVNQLINVTGIFQDI
                     YSDNSPENLSKQENIQELLKALHEFCERKEKEEDKGFLPDFLSEISLLTDQDTDKSEG
                     DRVTLMTVHSAKGLEFRAVFIVGMEEQLFPSIFSIFNERELEEERRLFYVAITRAKEH
                     CFISYAKSRFRNGKTYFSEPSRFIKDIDKQYLDLPSEETISQQTSTSWDDTLVTERKH
                     FYQQSNGQGTVENSFSQKRLVKLNDLKEEKVLIEKPSLPVGSLVKHSQFGIGKVIETC
                     IVNGNERAEIDFGEKGKKSLLLKFAKLEVLK"
BASE COUNT         1169 a          496 c          529 g          996 t
ORIGIN      
        1 tttcataaaa attctgaatt tcttgaaaat cagcatcaat atttcctgtt ggataaaaaa
       61 ttttggcaat acccaattcc ttctttttat aatcaatata agacaactga atggggacat
      121 ttgctcccaa agcaatataa taaaaacctt ttttccatgc tgttacacaa cctcgagttc
      181 cttcgggagt aatgaccaaa tggaaactgt caagtttctg aaatttatcg atcatttgtt
      241 gagtaagcga attgtgtttt gaacggtcaa taggaatgcc acctaacgat ttgaaaaaga
      301 aattcaatgg aaaaatgaac catgattttt tgataagaaa agaagctttt cgcccgatgg
      361 accaataaga cagttcacca attaaaaaat cccaattaga agtatgaggt gccaggcaaa
      421 ttacgctctt ttgtggctca ggcaaaggat atatcatttg ccagccaaaa tatttgaaaa
      481 tcaaacgtac taattttttc atcctatcgt cttttgcctt taaatttgat tatttcaaac
      541 tctttatatc aaaagtaata aaaaaattat tataagtatt ccaactttat aaaaattcaa
      601 gttgtaaaat tattatatct cagtcataat aagaaattaa ctcttcaaaa tttacgaaaa
      661 actattcctt ctacatttaa aagtggggta agtttattaa ctttgcaata agttaatgta
      721 atttttgttc ttccaaaaca gcataatata aatttttaaa tgattgaaag agagaaagat
      781 acaaattttg attttttaaa tgaactcaat gaaagtcaac gtctggcagt agaatatact
      841 gatggacctt cgctggtgat tgcgggtgct ggatcaggaa aaactcgtgt gctaacttac
      901 aaagtagctt atttactgaa aaaaggattt ccaccttctt ccattttggc cctcactttc
      961 accaacaagg cagctcgtga aatgaaagaa cgaatagctg aaattgttgg gaataaaatt
     1021 gctcgttatt tatggatggg tacttttcat tctatttttt cacgcattct acgggctgaa
     1081 gctcaatata ctggatttac acaaaatttt agcatttacg atcaatccga ctcaaaaagt
     1141 ctattaagaa gcattataaa agaaatggag ctggacagta aaatctatac tcctggttta
     1201 gttcaatccc gcatttcgat ggcaaaaaat aatctgataa cacctaaagc ctatgctgct
     1261 gattacagct taattgaaaa agatagaaaa aatcatatgg gtaaattatg tgatgtttat
     1321 cagacttatt gtattcgctg caagcaagcc aatgctatgg attttgatga tttattgcta
     1381 caaactaatt tgctgtttcg tgatcaccct gaagttttac aaaaatatca gcaacttttt
     1441 cgatatattc tggtggatga atatcaagat accaatttcg cacaatatct aattataaaa
     1501 aagctggctg aacgtcataa aaatatttgt gtagtaggag atgacgctca aagtatttat
     1561 tcttttcgcg gagcaaatat tgataatatt tttcaattta aaaattctta ccaggatgcc
     1621 aagattttca aactggaaca aaattatcgt tctacaaaaa ccattgtcaa ggcagccaac
     1681 agtttaataa agaaaaataa ggaacaaata caaaaaacta ttttttctga aaaagagcct
     1741 ggtgcaccaa ttaaagtctt tagcacttac accgacctgg aagaaggaat cgttgttgcc
     1801 aattgtgtag caaatctata ctcagaaaat tttcaatatc aggatattgc aattctttat
     1861 cgaactaatt cccaatcgcg ggtgctggaa gaagccttgc gaaatagaaa tattccttac
     1921 cgaatttatg gaggtttatc tttttatgaa agaaaagaaa taaaggatgt cattgcttat
     1981 tgcagattga tttgcaatca acaggacgaa gaagctttaa aacgtattat taattatcct
     2041 actcgtggca ttggcgaaac cactctgaac aagatcatcg aatgtgctca gcagaaccaa
     2101 ataagtctct gggaagtaat atcaaacttg gcagaatata aattaccaat caattcaaga
     2161 acggtaacaa aaattgaaca atttaaggaa ttaattaatg tttttgtagg acaagtaaac
     2221 acacaaaatg cttatgattt ggtcaatcaa ctcattaatg tcactggcat ctttcaggat
     2281 atttattctg acaattcgcc tgaaaatctt agtaaacagg aaaatattca ggaacttttg
     2341 aaagctctcc atgaattttg tgaaaggaaa gaaaaggaag aagacaaagg ctttttacct
     2401 gattttttat cagaaatttc tttattaact gatcaggata cagataaatc agaaggagac
     2461 agagtaactt taatgaccgt tcattctgca aaaggattgg aattcagagc agtttttatt
     2521 gttggaatgg aagaacaatt gttcccttct atttttagca tcttcaatga acgtgagttg
     2581 gaagaagaac gacgtttatt ttatgtcgct attacacgag ctaaagaaca ttgttttatc
     2641 agctatgcaa aatctcgttt cagaaacgga aaaacttatt tttcagagcc cagtagattt
     2701 atcaaagata ttgataaaca atatcttgat ttgccaagcg aagaaacgat ttcccaacaa
     2761 actagcacaa gttgggatga tacattagta actgaacgga aacattttta ccagcaatct
     2821 aatggtcagg gaacagtaga aaattctttc tctcagaaac gactggtaaa gttaaatgat
     2881 ttgaaggaag aaaaagttct tattgaaaaa ccttctcttc ctgttggcag tttagtgaag
     2941 cacagccagt ttggaattgg aaaagtaatt gagacttgta ttgtaaatgg caatgaacga
     3001 gctgaaatag atttcggcga aaaaggaaaa aaatctttat tgttgaaatt tgcaaaatta
     3061 gaagtgttga aataaaaatt ttttatcttt gtaaaacaat aattttctaa aatttacctc
     3121 aatcttcaat taaaaataaa aatttgaatt atcatggcag aaaaaacagc agacgaaata
     3181 aaaacaactt
//
LOCUS       DAOTKS010000179         3183 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_25111_length_3183_cov_3.966432, whole genome shotgun sequence.
ACCESSION   DAOTKS010000179 DAOTKS010000000
VERSION     DAOTKS010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25111_length_3183_cov_3.966432"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /locus_tag="P5236_04355"
     CDS             complement(<1..1095)
                     /locus_tag="P5236_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRU63626.1"
                     /translation="MQISEIQAVKQRFGIIGNSELLNRAIDIAVQVAPTDLSVLITGE
                     SGVGKENFPQIIHYYSQRKHGPYIAVNCGAIPEGTIDSELFGHEKGSFTGAMAERKGY
                     FEVADGGTIFLDEVGELPLSTQVRLLRVLEAGEFMKVGSSKIQKTDVRVVAATNFNIP
                     EALREGRFREDLYYRLNSVPIYIPPLRDRKEDIVLLFRKFAADFAEKYHMPPIRLTED
                     AKELLTNYYWRGNVRQLKNITEQISVIEHEREINAEILKAYLPKEEFERLPALLASHH
                     HNQKTFANEREILYQILFDMKQDMIELKKLVFNLMNGQKVEMQPGYFNVIPSYSPSSV
                     VDQSDNLITPPANFNLQKEDALTKKFSQNEK"
     gene            1558..2295
                     /locus_tag="P5236_04360"
     CDS             1558..2295
                     /locus_tag="P5236_04360"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HRU63627.1"
                     /translation="MRENFSYLIEHYNEKPEDKVIAKIKEMINSGILKPGDKLPAERK
                     LASELGIGRTHIREALHKLEFYGIIKTLPQSGSIIQGLDINTLDGLISDVLNLQDYDF
                     YSLVETRCVLEMSAIKFCAKRRTTKDIQSLEIAHENFVKFFEDKNKRVSYDFAFHRNI
                     AEGSHNPVIKAMLLIITPDIMLIYNRDRICDSATNVIEEHAQMLNAIKDQEGELASAL
                     MLQHLQGVMNFAKSKLKTNYVENISLI"
     gene            2533..>3183
                     /locus_tag="P5236_04365"
     CDS             2533..>3183
                     /locus_tag="P5236_04365"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRU63628.1"
                     /translation="MKKSKYKKFIKKGWLILFLLGINATPIFSQIKVSGIVMDINNEP
                     LIGVNITIKGTQKGTISDFNGKYSIEVPNQKSILVFSYLGYKTTEKTIPSNGILNVIL
                     EEEIQSLDEIVVVAYGTQKKSHLTGAVSSLKNEKMDEIPVSRVDQALQGKIAGVQINN
                     VNPEAGAAPKIRVRGLGSISASNDPLVVVDGFPIPDGLSMVSMADVQSIEVLKDASS"
BASE COUNT         1137 a          490 c          498 g         1058 t
ORIGIN      
        1 tttttcattc tgcgagaatt tcttagttaa agcatcttct ttttgaagat taaaattagc
       61 aggaggtgtt atcaaattgt ctgattgatc caccacggaa gatggagaat aagaaggaat
      121 aacattaaaa taccctggtt gcatctctac tttttgacca ttcattaaat taaaaactaa
      181 ttttttcagc tctatcatat cctgtttcat atcaaaaagg atttgataaa gtatttcacg
      241 ctcatttgca aatgttttct gattgtgatg atgcgaagct aacaaagcag gtaaacgttc
      301 aaattcttct ttcggtaagt aagcctttaa aatttctgca ttaatttctc gttcatgttc
      361 aattacagaa atctgttcgg taatattttt aagctgacga acatttcctc tccaataata
      421 gtttgttagt agttctttag catcttcagt taatctgata gggggcatat ggtatttttc
      481 agcaaaatct gcagcaaatt tccgaaacaa caatacaata tcttcttttc gatctcttaa
      541 aggcggtata taaatgggaa cagaattgag ccgataatat aaatcttctc tgaaacgacc
      601 ttcacgaaga gcttcaggga tattaaaatt cgtagcagca actaccctaa catcagtctt
      661 ttgaatttta gaagatccta ctttcataaa ttcacctgct tcgagaaccc gcaatagccg
      721 aacctgagtt gaaaggggta attcgccaac ttcatccaaa aaaatggtac caccatcagc
      781 cacttcaaaa taaccttttc gctcagccat agcaccggta aaagaacctt tttcatgacc
      841 aaacaattcg gaatcaattg ttccttcggg aatagctcca caatttacag ctatgtaggg
      901 accatgtttt cgttgactgt aataatgaat aatctgagga aaattttctt ttcccacacc
      961 actttctcca gtaatcaaaa cggataaatc agttggagca acctgaactg caatatctat
     1021 agctcggttc aaaagttctg aatttccaat gatgccaaac cgttgtttca cggcttgtat
     1081 ttctgaaatt tgcatgctta cttcctttct ttaatatgtt tcctgtaatt ctgacataaa
     1141 actactgaca aaattagtaa aaaataatga aaagttattg gtaaaaaaat aaaagttgta
     1201 ttttttgaag gattatatat caaagtaaac ttttatgtaa cataaaaaac cagtatacat
     1261 taatatttca tacctataaa caaaatatga aactgtttcg aaaagtctgt tttaataatt
     1321 tctttttaaa agttgatatt gatattcaat agattaaaaa gcagcaagcc tattacaatt
     1381 gatttttcag aagggactca actttaaatt ttttaaaaat ttatcacaat atcatttttt
     1441 ctttataatt ttggtgtacc aattcttgaa aagttttatt aaagaaattc gtgttaagaa
     1501 taaagaaaca attggatttt taacttttat aaaaaattga attaatcata tttaattatg
     1561 agagaaaatt tcagttattt aatagagcat tataatgaaa aacctgaaga taaagttatt
     1621 gcaaaaataa aagaaatgat taattcaggt attttaaaac caggtgataa attacctgct
     1681 gagagaaaat tagcatctga attgggtata ggaagaacgc atatacgaga ggctttacac
     1741 aaacttgaat tttatggaat tattaaaact ttaccacaaa gtggttcaat aattcaagga
     1801 cttgatatta ataccttaga tggcttaatt tcagatgtat taaaccttca agattatgat
     1861 ttttattctc tagtagaaac aagatgtgtg cttgaaatga gtgctataaa attttgtgct
     1921 aaacgtagaa ccacaaaaga tattcaatca ctagaaattg ctcatgaaaa ctttgttaaa
     1981 ttttttgaag ataaaaataa aagggtaagc tatgattttg cttttcatcg caatatagcg
     2041 gaaggtagcc ataatccagt aattaaagct atgcttttaa ttattactcc agatataatg
     2101 ttgatatata atagagacag gatttgcgac agtgccacca atgttataga ggaacatgct
     2161 caaatgctaa acgccataaa agaccaggaa ggtgaattag cttctgcctt aatgttgcaa
     2221 cacttacagg gggtaatgaa ctttgcaaaa tcgaaattaa aaactaatta tgtggaaaat
     2281 ataagtctca tctaagaaat tttattcttt cctttcaatt aaatcgattc taatttttag
     2341 cattataaaa ataagttagg gttaattaat tagcttaagg aattcaaaat atgttttaca
     2401 acatattttg aaaaataatt ctaaaaaaat ttgcttccta ataaaaatca cattattttt
     2461 ggtcgaccaa tttttggtcg accaattttt tatttattca ttcataataa ctttttattt
     2521 aataaagaac gcatgaaaaa atcaaagtac aagaaattta tcaaaaaagg atggctgata
     2581 ctttttctgt taggaataaa tgctacccct attttttctc aaataaaagt aagtgggatc
     2641 gtaatggata ttaataatga gccattaata ggagttaata ttactattaa agggactcaa
     2701 aagggtacta tttcagattt caatggaaag tatagtatcg aagttccaaa tcaaaagtcc
     2761 attcttgtat tctcttacct tggttataaa acaacggaaa aaactattcc ttcaaatgga
     2821 attctcaatg tgattttaga agaagaaatt caatcccttg atgaaatagt tgttgtagct
     2881 tatggaacac agaaaaaatc tcacttaact ggtgctgtct cttcattaaa gaatgagaaa
     2941 atggacgaaa ttccagtttc acgagtagat caggctctac aaggtaaaat tgccggtgta
     3001 cagataaata atgtaaatcc tgaagccgga gcagcaccta aaatccgtgt tcgtggtctg
     3061 ggatccatca gtgccagcaa cgacccgtta gtagtagtag atggttttcc cattcctgat
     3121 ggcttatcga tggttagtat ggcagatgtc cagtcaattg aagtattgaa agacgcttct
     3181 tct
//
LOCUS       DAOTKS010000180         3175 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_25183_length_3175_cov_4.511538, whole genome shotgun sequence.
ACCESSION   DAOTKS010000180 DAOTKS010000000
VERSION     DAOTKS010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25183_length_3175_cov_4.511538"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..1147)
                     /locus_tag="P5236_04370"
     CDS             complement(257..1147)
                     /locus_tag="P5236_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63629.1"
                     /translation="MKKKNLTIIIILSAVVLILGIFLIYNYKKTKEKEAEMAEMVEMM
                     NFEKEQLEKDYSDLALEYDTYSSKISNDSLVQLLQKEKMKVQQLLEELKITKATNAKR
                     IAELKNELTVLRTVMTNYVHQIDSLNAANKLLRTENIEVKRKYEQVNETVKQLAKEKE
                     TMKEVITRASMLEITNFSMTPLDSKNRKTQRYSKITTLQFNYTISKNPTTQPGNKTLY
                     LRITQPDEEVLIKSPDDLFPYEDKQIAYSVKKDFEYSGEAISDVIYWKVDRVLQIGTY
                     RADFFVDGFRIGSFTFEIEK"
     gene            complement(1201..2511)
                     /gene="murF"
                     /locus_tag="P5236_04375"
     CDS             complement(1201..2511)
                     /gene="murF"
                     /locus_tag="P5236_04375"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRU63630.1"
                     /translation="MEISELYQLFLRHPVICTDSRNCVPDSLFFALKGKNYDANIFAL
                     SALEKGCSYAVVDDKKYVIDERFILVKDVLQSLQELARFHRKQFDIPVIAITGTNGKT
                     TTKELISMVLMEKYNLLSTEGNLNNHIGVPLTLLQLKPEHQIAIIEMGANHPGEIKKL
                     AEIASPNYGLITNVGKAHLEGFGSLEGVMQAKAELYEYIYQEGEAIFINKKNQYLQQM
                     AEKAGFTDVSRKIEYCLNPIEKNVNITGEIVDCTPYLHMKCTTNEGTFEIKTKLIGDY
                     NAENVLAAAAVGNFFGVHNEQIKHGIENYQPQNNRSQFSVTQHNKLIIDTYNANPTSM
                     QAAILNFADMKFSPKTLILGDMLELGEQSEEEHQNIINLLQQKNFQNVFLVGEQFSKV
                     NKAYPSFENVEDLIIYLKQHPLFDQYILIKGSRGIYLEKVLPFL"
     gene            complement(2646..3038)
                     /gene="secG"
                     /locus_tag="P5236_04380"
     CDS             complement(2646..3038)
                     /gene="secG"
                     /locus_tag="P5236_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRU63631.1"
                     /translation="MYLLFTILIVVASILLVLVVLVQNSKGGGLAAGFSSSNQFMGVR
                     KTTDFLEKATWTLIAFIVVFSVAAAGIHSNQTGGNKESEIKEQYQKAQKEVPGAVIPD
                     FGELPENAPSSPEKNNGGELPKIPTPEK"
BASE COUNT          971 a          547 c          487 g         1170 t
ORIGIN      
        1 gcgaaatgtt tggttttatg agcggttaaa atcaggagat tttggcagtg gtaaatggct
       61 gacgccagtg agaaaatctg gagatttttt acttacgacg tagcctggag gctacgccga
      121 acaaggaaaa tctggagaat ttttttactt atgacgtagt ctggaggcta cgccggacac
      181 aaggctacgc cgaacgataa ggtaaaaata tatacagtaa aaaaacaata ttttttttaa
      241 tctttattta ggtaatttac ttttcaattt caaaagtgaa acttccgatc ctgaagccat
      301 caacaaaaaa atcagcacga tacgttccaa tttgtaggac gcgatctact ttccaataaa
      361 taacgtcact tatagcttca cctgaatatt caaaatcttt ctttacagaa taggcaattt
      421 gtttatcttc gtatgggaaa aggtcatcag gactttttat caatacctct tcatctggct
      481 gagtgattcg taaatacaag gttttgtttc caggttgagt tgtaggattt ttactaatag
      541 tataattaaa ttgtaacgta gtaattttgc tgtatcgctg tgtttttcta tttttactat
      601 ccaatggtgt catgctgaaa ttggtaattt cgagcataga agcacgagta attacttctt
      661 tcatagtttc cttttcttta gcaagctgct tgacagtttc attcacctgt tcatattttc
      721 gttttacttc tatattttct gttcttaata acttattggc agcattcaat gaatctattt
      781 gatggacata atttgtcatt actgttctga gcaccgtcaa ctcatttttt agttctgcaa
      841 ttcgtttggc atttgttgct ttggtaattt ttagttcttc caacaattgt tgcactttca
      901 ttttttcctt ttgcagcagt tgtactagtg agtcgttgct tatttttgaa gaataagtgt
      961 cgtactccag agctaaatct gaatagtctt tttcaagttg ttctttttca aaattcatca
     1021 tttcaaccat ttcagccatt tcagcttctt tttctttggt tttcttataa ttatagatta
     1081 agaaaattcc taatataaga acgactgctg ataaaatgat aatgatagtc aagttttttt
     1141 tcttcatgtc tcaaaattca ttttttgatc tactaaaaaa tcaattattt cgttgtcatt
     1201 ctataaaaaa ggcaatacct tctctaaata aattcctctg gagcctttaa taagaatata
     1261 ctgatcaaac agcggatgct gctttaaata aattatcaaa tcctccacat tttcaaaaga
     1321 aggataagct ttatttactt tggaaaattg ttctcccacc agaaaaacgt tctgaaagtt
     1381 tttttgttgt aacaaattga taatgttttg atgttcttct tcactttgtt ctcctaattc
     1441 taacatgtcg ccaagtatta aggtttttgg cgagaatttc atatctgcaa aattcaaaat
     1501 agctgcctgc atactggttg gattggcatt gtaggtatca atgatcaact tattatgttg
     1561 agtaacacta aattgtgaac gattgttctg tggttggtaa ttttctattc catgttttat
     1621 ctgctcattg tgtacaccaa aaaaattccc taccgcagca gcagctaaaa cattttcagc
     1681 attgtaatca ccaattaatt tcgtttttat ttcaaatgtt ccttcgttgg tggtgcattt
     1741 catatgtaaa taaggagtac aatctactat ttcacctgta atattaacat tcttttcaat
     1801 tggattcaaa caatattcaa tttttctact aacatcagta aaaccagctt tttcagccat
     1861 ttgttgcaaa tattgattct ttttattaat aaaaatagct tccccttcct gatagatata
     1921 ttcgtacaat tctgcttttg cttgcattac tccttccaat gatccaaaac cttccagatg
     1981 agcttttcca acattagtaa tcaagccata attgggagaa gcaatttcag ctaatttttt
     2041 gatttctcct ggatgattgg ctcccatttc gataatggct atttgatgtt caggctttaa
     2101 ttgcagtaga gtgagtggaa cgccaatatg attgttcaaa tttccttctg tggacaataa
     2161 attatatttt tccatcagaa ccatgctgat caattccttt gttgtagttt ttccattggt
     2221 tccagtgata gctataactg ggatatcaaa ttgtttacga tgaaaacgag ccagttcctg
     2281 caaactttgt aaaacatctt ttactaaaat aaaacgttcg tcaataacat attttttatc
     2341 atccaccact gcataggagc atcccttctc aagagctgat aaagcaaaaa tgttcgcatc
     2401 ataatttttc ccttttaaag caaaaaataa tgagtcaggc acacaatttc tactatccgt
     2461 acaaataaca ggatgtcgaa gaaataactg gtacaattct gaaatttcca taatgtgaat
     2521 gaataattta caaaaataaa gataatctga taaactgaag gaaggttaac ataaatttta
     2581 acaaaaaagg atggttgttt ttattgccat cctctttaaa atacatttaa tatttgaatt
     2641 tttctttatt tttcaggggt aggaatttta ggaagttcac caccattatt tttttctgga
     2701 gaagaaggtg cattttctgg taattcgcca aaatcaggaa ttacggctcc aggtacctct
     2761 ttttgagctt tttggtattg ctccttaatt tcagattctt tatttccgcc tgtttgatta
     2821 gaatgaatgc ctgctgcggc aactgaaaaa actacaataa aggctatcag tgtccatgtt
     2881 gctttttcaa gaaaatcagt ggttttacga acacccataa actgattgga agaagaaaag
     2941 cctgctgcca gacctccacc ttttgaattt tgtaccaata ctactaaaac aagtaatatg
     3001 gaagctacta caattaaaat ggtaaacaaa aggtacataa ctatcggata attatttttt
     3061 taaatattca atcacctttt ccaaaaaacg aatttggtct gcaaagtaaa tacttttttc
     3121 tggattattc aaatttaatt ccttcaaaat ttttactgcc tcttcataat ttttc
//
LOCUS       DAOTKS010000181         3166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_25262_length_3166_cov_4.315333, whole genome shotgun sequence.
ACCESSION   DAOTKS010000181 DAOTKS010000000
VERSION     DAOTKS010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25262_length_3166_cov_4.315333"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1238
                     /locus_tag="P5236_04385"
     CDS             <1..1238
                     /locus_tag="P5236_04385"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRU63632.1"
                     /translation="IKKGTFLMGANDQSAIGAINDKSIQVTVDAFWMDQTEITNDEYK
                     QFVNWVRDSIALRSLVMGGRDEFRLKLKNQEDLPPEIAPLNWKIRIPWNSKDEEINDI
                     LNSFYYESENELSIKRQINPDKLKYKYEWVNYDQAALAKNKYDVNTGAYPTEAKVRVD
                     TSFVDENGVIHHQTIERKLKTRRDLISTKIVNIYPDTIMWIRDFQYAYNDPKMQMYFS
                     HPGFSQYPVVGVTWEQAQAFCHWRTHLFNSKSSVSAQDYRLPTEAEWEYAARGGRPMA
                     LYPWGGNYVRDKRGCFMANFKPMRGNYTEDTGTTTMKVGSFLPNDFGLYDMAGNVAEW
                     TSTAYNVSSNNLVYDINPSFQYNAKNSDPDVLKRKVVKGGSWKDIAYYLQCGTRTYEY
                     QYESRPYIGFRCVRSYNGE"
     gene            1273..2127
                     /gene="gldL"
                     /locus_tag="P5236_04390"
     CDS             1273..2127
                     /gene="gldL"
                     /locus_tag="P5236_04390"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HRU63633.1"
                     /translation="MASQFSIWWNSPKGKKVVNAAYSVGASIVILGAMFKILHLPNAN
                     YILGAGMICESLIFFSGAFDKPFKEYDWDKLFDFENGKPRFQGGNLAVQNILPRVDLN
                     YSETIDENDIKKLSEGIKNLSTTAQQLASLSNVVISTEKFAQNIESASSTTLNLIEKQ
                     KEFTNALQNISVVYENSKIEMEKEQEETKQSLQTINKNLASINTIYEIQLKNIQDQLT
                     EISQQNEHLKNTNEELEDISNNMQKINSVSNDILLQAEEYKSETTKLSQKIVELNNIY
                     GNMLNALS"
     gene            2177..>3166
                     /gene="gldM"
                     /locus_tag="P5236_04395"
     CDS             2177..>3166
                     /gene="gldM"
                     /locus_tag="P5236_04395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HRU63634.1"
                     /translation="MSASKNCPETTRQRMIGMMYLVLTAMLALNVSSEVLNGFSMVDN
                     SLHNTIQSSIVRNTGLYDDFQNQFRQNPTKVKEWLDKAILVKQKSDSLFDYLQDFKYQ
                     IVKRADGKKADPTARDIINKENYDIPGEYALVEGNGKILKQKIDSYRDFIIQLSENNP
                     TKQKMYATVFSTAPIGDKPWETAMFEMMPVSAVVTILTKYQSDIRMTEAEVVQYLKSQ
                     TDISDFRVNKIEALVVPNTRYVIRGGKYSAQIILSAIDSTKTPQYFVGNTPVPNGIYE
                     ITCTRPGTFTYSGYIQLIDNQGIIRKYPFQSDYIVGEPSATISNEDLNVVYRGV"
BASE COUNT         1182 a          481 c          548 g          955 t
ORIGIN      
        1 ttatcaaaaa aggaactttt ttaatgggag ctaatgatca atctgcaatt ggagcaatca
       61 acgacaagtc aatccaggta acggtagatg ctttctggat ggatcaaaca gaaataacga
      121 acgatgaata caagcagttt gtaaattggg ttcgtgattc tatcgcactt cgctcattag
      181 tgatgggtgg cagagacgag tttcgcttga aattaaaaaa ccaagaagat ttaccacctg
      241 aaatagctcc tttaaattgg aaaattagaa ttccatggaa ttctaaagat gaagaaataa
      301 atgacatctt aaattctttt tattatgaaa gtgaaaatga actttctatc aaaagacaaa
      361 ttaatcctga taaactaaaa tataaatatg aatgggttaa ttatgatcag gctgcgttag
      421 caaaaaacaa atatgatgta aatacaggag cttatcctac tgaagccaaa gtaagagtgg
      481 atacttcatt tgtagatgaa aatggcgtga ttcatcacca gactattgaa cgaaagttga
      541 aaactcgaag agatttaatt tccaccaaaa tagtcaatat ttatcctgat accattatgt
      601 ggatcagaga ttttcaatat gcttacaatg atcccaaaat gcagatgtat ttttctcatc
      661 cagggttttc acaatatccc gtagtgggtg ttacctggga acaagctcaa gcattctgcc
      721 attggcgaac acatttattt aacagtaaaa gtagtgtaag tgctcaagat taccgtttac
      781 ctactgaagc agaatgggaa tatgctgcac ggggtggcag accgatggca ctttatccct
      841 ggggtggaaa ttatgtaaga gacaaaaggg gatgttttat ggcaaacttt aaacctatga
      901 gaggtaacta tactgaagac acaggaacaa ctaccatgaa agtgggttca ttccttccca
      961 atgattttgg cttatatgat atggctggaa atgttgctga atggacatca accgcttaca
     1021 atgtatcaag taataatctg gtatatgata ttaatcccag ttttcaatat aatgctaaaa
     1081 atagtgaccc cgatgtgcta aaacgtaaag tggtaaaagg tggctcgtgg aaagacattg
     1141 cttattattt acaatgtggt acaaggactt atgaatacca atatgaaagt cgcccttata
     1201 ttggatttag atgtgtaaga tcatacaacg gcgaataatt ttaaaattca aaaacaataa
     1261 aagataaatt atatggcatc tcaattttca atttggtgga attcaccaaa aggcaaaaag
     1321 gtagttaatg cagcttatag tgtaggagcc tcaatagtaa tattaggagc tatgtttaaa
     1381 atattacatt taccaaatgc caattacatt ttaggagctg gaatgatttg tgaatcgctc
     1441 attttctttt ctggggcttt tgataagcct tttaaagaat atgattggga caaattattt
     1501 gattttgaga atggaaagcc acgctttcaa ggcggtaacc ttgcagtaca aaatattctt
     1561 ccacgagtag atttgaatta ttctgagaca atcgatgaaa atgatataaa aaaattatct
     1621 gaaggaataa aaaatctatc cacaacggct caacaacttg cttctctctc caatgttgta
     1681 atttcaactg aaaaattcgc tcaaaacatt gagtctgctt caagtactac tcttaatttg
     1741 atagaaaagc aaaaggagtt tacaaatgct ttacaaaaca tttcagtagt ttatgaaaac
     1801 tctaaaattg aaatggaaaa agaacaagaa gaaacaaaac aatctcttca aacaattaat
     1861 aagaacctcg cttccattaa tactatttat gaaatacaat tgaaaaatat acaagatcaa
     1921 ctcacagaga tatctcaaca aaatgaacat cttaaaaata caaacgaaga attagaagat
     1981 atttcaaata atatgcagaa aatcaactct gtaagtaatg atattttatt gcaagccgaa
     2041 gaatataagt cggagacaac aaaattatcc caaaaaattg ttgaattaaa taatatttat
     2101 ggcaatatgc tgaatgcctt gagctaacct aatgctattt tcttaactta acatgtaact
     2161 taatttcagt aaactaatga gtgcttcaaa aaattgtcca gaaaccacca gacaaagaat
     2221 gattggtatg atgtatcttg tattaacagc gatgttagca ttaaatgtat caagtgaagt
     2281 cttaaatggt ttttcaatgg tcgacaatag tttacataat actatacagt cttcaatcgt
     2341 aagaaatacc ggtctatatg atgatttcca aaatcaattt cgacaaaatc caactaaagt
     2401 taaagagtgg cttgataaag caatattagt taaacaaaaa tctgattctt tgtttgatta
     2461 tctccaagat tttaaatatc aaattgttaa aagggccgat ggtaaaaaag ctgatcctac
     2521 tgctcgggac attattaata aagaaaatta tgatattcca ggagaatatg cattggtaga
     2581 agggaatggt aaaatcttaa aacaaaaaat cgattcttac cgggatttca ttattcaatt
     2641 atcagaaaat aatcctacaa aacaaaaaat gtatgcaact gttttttcta ccgctcctat
     2701 tggtgataaa ccatgggaaa ctgcaatgtt tgaaatgatg cctgtatcag cagtagtaac
     2761 aatattaaca aaatatcaaa gtgatattag aatgactgaa gcagaagtag ttcaatattt
     2821 aaaatctcaa actgacattt cagattttag agttaataaa attgaagcat tggttgtgcc
     2881 aaatactcgt tacgtgattc gaggaggaaa atatagtgcc cagattattc tttcagcaat
     2941 tgattctact aaaactcctc aatattttgt tggaaatact ccagttccaa atggaattta
     3001 tgaaattaca tgcactcgtc caggaacttt tacctattct ggttatatac agttaataga
     3061 caatcaagga attattcgga aatatccttt tcaaagtgat tacattgtag gtgagccatc
     3121 agccactatt tctaatgaag atttaaatgt ggtttataga ggtgtc
//
LOCUS       DAOTKS010000182         3163 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_25293_length_3163_cov_4.614865, whole genome shotgun sequence.
ACCESSION   DAOTKS010000182 DAOTKS010000000
VERSION     DAOTKS010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25293_length_3163_cov_4.614865"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..1146
                     /locus_tag="P5236_04400"
     CDS             25..1146
                     /locus_tag="P5236_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRU63635.1"
                     /translation="MDLFNVYPLFNIEIVKGSGCRTYDKQGNEYLDFYGGHAVISVGH
                     SHPYFVKKLTEQIRKIIFYSNSVINPLQIKLADKLGKISGYEDYSLFLINSGAEANEN
                     ALKLASFHTGRKKVIAFSKSFHGRTSAAVSVTDNPSIVAPINENFEVEFFPLNDTVAV
                     KKSLRKRDVCAVIIEGIQGVGGIQIPNADFLQVLSAECKATGTILILDEVQSGYGRTG
                     KFFAHQYAGIRPDLITVAKGMGNGFPIGGVIISPMFKPIYGMLGTTFGGNHLACTAAL
                     AVLDIIEEEHLMENAKKIGDYLLDELQKFPRIKEVRGVGLMIGIEFEEPVSALRTKLL
                     YDKKIFTGVSGFNIIRLLPPLCLSKEEADLFLERFYELL"
     gene            1155..>1841
                     /locus_tag="P5236_04405"
     CDS             1155..>1841
                     /locus_tag="P5236_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63636.1"
                     /translation="MKTHFLLLIDFFIFLSLLNLSAQEIVHDKLILKSGDIYVGEIIL
                     KNDEIVILQTTDGTRYQFPFSDIKEISKSSDIFPTKNISSNNDTLSFPFCAFLEFTGG
                     VAQAKNKFDLSPNSQITLIFGKKQLFNKGFFIGGAIGYNNTSLEQNNSLNFLPIFIRL
                     EDIPIKKRLSPYMTLDAGYAFALASDYSGGTMTKISGGITYKLTYKTIVFSGIYAGIQ
                     NFKGLLTETNA"
     assembly_gap    1842..1941
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1942..2627)
                     /locus_tag="P5236_04410"
     CDS             complement(<1942..2627)
                     /locus_tag="P5236_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRU63637.1"
                     /translation="MMASLYLIPTPLGTTSLEKIFPPYNIEIINRLNHFVVEDVRTVR
                     RFLKQISQERNINEVKFYLLNEHSSKEELVSLLEPLYNGFDLGLMSEAGCPAVSDPGA
                     DLVALAHKNNFTVIPLIGPSSVLLSLMASGFNGQNFEFVGYLPVQSEERIKALRRLEA
                     KVYAENQTQIFIETPYRNMKMLETILKVCQPSTKLCIAADITLESEFIKTKSIGEWRK
                     NLPDLQNKPC"
     gene            complement(2947..>3163)
                     /locus_tag="P5236_04415"
     CDS             complement(2947..>3163)
                     /locus_tag="P5236_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007900485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HRU63638.1"
                     /translation="HFLLIPGIGVQGGNLEEVASAGLNEGCGLLVNSSRQIIYASSEK
                     DFASAAQVAAKKLQTTMEYLLKQHGII"
BASE COUNT         1027 a          478 c          513 g         1045 t
ORIGIN      
        1 atttttatct ttaatttaat cgatatggat ttatttaatg tttatccact ttttaatatt
       61 gaaattgtaa aaggttcagg ttgtagaact tacgataagc aagggaatga atatctcgat
      121 ttttatggtg gtcatgcagt aatttcagtg ggtcattctc atccttattt tgtaaagaaa
      181 ttaactgaac aaatacgaaa aattattttt tattccaatt cagtaataaa tcctttgcaa
      241 attaagcttg ctgacaaatt aggaaaaatt tctggttatg aggattattc cttgttttta
      301 attaattcag gagcagaagc caatgaaaac gccttaaaat tagcttcttt tcatacagga
      361 agaaaaaaag ttattgcttt tagcaaaagt tttcatgggc gcacttctgc tgctgtcagc
      421 gtaacagata atccttctat tgtagctccc attaatgaga attttgaagt tgaatttttc
      481 cctttaaatg atacagtggc agtaaaaaaa tctttgagaa aaagggatgt ttgtgcggtt
      541 attattgagg gaattcaggg agtaggaggt atacaaattc caaatgcaga ttttttacaa
      601 gttctcagtg cagaatgtaa agctacaggt acaatactta ttttagacga agtccaatca
      661 ggttatggaa ggacgggtaa attttttgct catcaatatg ctggcatccg ccctgatctt
      721 atcaccgtcg caaaaggaat ggggaatggc ttccctattg gtggagtaat tatcagcccc
      781 atgtttaaac ctatttatgg tatgcttgga actacctttg gaggaaatca tttggcttgc
      841 actgctgctc ttgcagtgct ggatatcata gaagaagaac acttgatgga aaatgctaaa
      901 aaaataggtg attacctctt ggatgaatta caaaaatttc ctcgaattaa agaagtaaga
      961 ggagtaggat taatgattgg tattgaattc gaagaacctg taagtgcttt aagaacaaaa
     1021 ttattgtatg acaaaaaaat ttttacagga gtgagtggtt ttaatattat tcgtttactt
     1081 cctccattgt gtctttctaa agaagaagca gatctgtttt tggaacgttt ttatgaatta
     1141 ctttaatttg tcggatgaaa actcattttt tgttattaat agattttttt atttttcttt
     1201 ctctcttaaa tttatctgca caagaaatag ttcatgataa acttatttta aaaagcggcg
     1261 atatttatgt gggtgaaatt attttaaaaa acgacgaaat agtaatatta caaaccaccg
     1321 atggaactcg atatcaattt cctttttctg atattaaaga aattagtaaa tcatccgata
     1381 ttttcccaac aaaaaatata tcttctaaca atgataccct ctcttttcct ttttgtgctt
     1441 ttttagaatt taccggtgga gtagcacagg caaaaaataa atttgattta tcacctaatt
     1501 cacaaataac attaattttt gggaaaaagc aattgttcaa taagggcttt ttcattggtg
     1561 gtgcgatagg atacaacaac acttcgctgg aacaaaataa ttctttgaac tttctaccaa
     1621 tttttattcg tttagaagac attcccataa aaaagaggtt gtctccttat atgacattag
     1681 atgctggtta tgcttttgct cttgcttctg attacagcgg tggaacaatg actaaaattt
     1741 caggaggaat tacctataaa cttacgtata aaacaatagt tttcagtgga atttatgctg
     1801 gcattcagaa ttttaaaggt ttgttaacag aaacgaatgc annnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn natgcacggt ttattttgaa gatcaggcaa gtttttccgc
     1981 cattctccaa tgctttttgt tttaataaat tcactttcta aagtaatgtc cgctgcaata
     2041 cataatttag tagaaggttg gcatactttt aaaatagttt ccaacatttt catgtttctg
     2101 taaggagttt caataaaaat ttgtgtttga ttttcagcgt atacttttgc ttcaagtcga
     2161 cgaagtgctt tgattcgttc ctcagattgg acaggtaaat aaccgacaaa ttcaaaattt
     2221 tgtccattga accccgaagc catcaacgaa agcaaaacag aagatggacc aatcaaaggg
     2281 atcacggtaa aattgttttt atgtgccaaa gcaactaaat cggcgccagg atctgacacc
     2341 gctggacacc ctgcttcaga catgagtccc aaatcaaaac cattgtacag tggttctaat
     2401 aaggaaacta actcttcttt tgaagaatgt tcatttagca aataaaattt cacttcattg
     2461 atatttcttt cctgagaaat ttgtttcaaa aatcttctaa cggttcgcac atcttctacc
     2521 acaaaatggt tcaatcggtt aataatttct atattgtaag gaggaaatat tttttctaac
     2581 gaggtcgttc ctaacggtgt gggaatgaga tataaacttg ccataatttt tttgaaaaat
     2641 gtgaacaata aaggagcaaa actaaagaat taataaagat aaaaaatgat tttataccaa
     2701 attgttatca ttaattttaa tttgaatagt cataacagaa tattaacatc aaaccgatgt
     2761 gaaattgaat gcgtttttca aaaggatgct tcgcttcaaa actatttcct ttatgctctg
     2821 cagattactg tctatgcaaa tccaaaggca aaaaggtggc acaaattgta aatttgagtc
     2881 agtagtaagt actttctttt tattcaacaa taaataaaat ttttaagctt aacaataata
     2941 aaaacattaa attattccat gttgtttaag caagtattcc atagtagttt gcaacttttt
     3001 tgctgctacc tgagcagcac tggcaaaatc tttttcagaa gaagcataaa taatctgtcg
     3061 tgaagaattt accaacaatc cacaaccttc atttaaacca gccgatgcca cttcttccaa
     3121 atttccacct tgtacaccaa ttccaggaat taataagaaa tga
//
LOCUS       DAOTKS010000183         3138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_25532_length_3138_cov_5.077522, whole genome shotgun sequence.
ACCESSION   DAOTKS010000183 DAOTKS010000000
VERSION     DAOTKS010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25532_length_3138_cov_5.077522"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..371
                     /gene="rpmF"
                     /locus_tag="P5236_04420"
     CDS             186..371
                     /gene="rpmF"
                     /locus_tag="P5236_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRU63639.1"
                     /translation="MAHPKRKHSKQRTAKRRTHDKAKIPTLAICPNCGATYIYHTVCG
                     ECGYYRGKLAIEKSASV"
     gene            455..1456
                     /locus_tag="P5236_04425"
     CDS             455..1456
                     /locus_tag="P5236_04425"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007757562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRU63640.1"
                     /translation="MAKINAVITGVGGYVPDYILTNDELSTMMDTSDEWITSRVGIKE
                     RHVLKDKDLGTSFMAVKAIEDLFQKTGTKPEEIEMIICATATPDYILPSCASIIAEKV
                     GIKNAIAFDLEAACAGFIVALTTAAAFIESGRYKKILAIGAEKFTSLTNYEDRTTAPL
                     FGDAAGVVLVEPTEEEVGFIDSVLFTDGIGFSHLGTNGGISTHPPSHETVDKKMFTIY
                     QEGQYVFKHAVSDMSDAVVKIMERNHLTKDDVNWLIPHQANLRIIDAIAQRVGLDYEK
                     VIININKYGNTSAASIPLCLWEYENKFKKGDNIILTAFGAGFTWGAVYLKWGYSKQE"
     gene            complement(1506..2228)
                     /locus_tag="P5236_04430"
     CDS             complement(1506..2228)
                     /locus_tag="P5236_04430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRU63641.1"
                     /translation="MLDINKIYNISCLDGLKQIPENTIDLIFTDPPYYQYRAQNIQGL
                     KNHKDLVTEFDFDHFDSEKDYLDFLRRVLAECYRVSKEGASGYLWCGDDFVSYFNRLV
                     EQVGFQFRKVIHWHKTNPFPAISSRKMFSNSMEMLVHFSKGTPKTWNYKSVNEMHNFI
                     EAPICMGKERTRHKTQKPLKVCIPFIEISSNVGDLILDPFIGSGTTAVACKMLKRNFI
                     GFEINEEFCKIAENRILHTKNE"
     gene            complement(2271..>3138)
                     /gene="meaB"
                     /locus_tag="P5236_04435"
     CDS             complement(2271..>3138)
                     /gene="meaB"
                     /locus_tag="P5236_04435"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HRU63642.1"
                     /translation="QEIIEKCLPYAGKSIRVGITGVPGAGKSTFIETLGMYLVRSGHK
                     LAVLAIDPSSERSKGSILGDKTRMEELSASKNAFIRPSPSAGTLGGVARKTRESIILC
                     EAAGFDTIFVETVGVGQSETAVHSMVDFFLLIQIAGAGDELQGIKRGIMEMADGIVIN
                     KCDGNNIDKAQIAKIQHQNALRLFPPTESGWSPEVLTCSALERKGIDEVWNMIERYVQ
                     FTKANGYFEYKRNNQSKYWMYETIDEQLKVNFYQNEEIQKKLIEVEKKVLANEISSFM
                     GAKILLDLYFKK"
BASE COUNT         1041 a          545 c          505 g         1047 t
ORIGIN      
        1 cgccagggga atgtaacaaa acaatgataa ataaactaaa aattcacata gtgcgtccta
       61 cagaagaaga tgttgaaggc gaatcatttg acatggatga agataatgat tttttatcac
      121 aagaaccaac tacagatttc aattgggatt tacaaaatgt tgataattaa ataaataata
      181 gaaaaatggc acatccaaaa agaaaacatt caaaacaacg tacagctaaa agaagaactc
      241 acgataaagc aaaaattcca acattagcta tttgtccaaa ttgtggcgcc acttatattt
      301 atcatactgt ttgtggcgaa tgtggttatt atcgaggaaa attagctata gagaaatctg
      361 catccgtttg aaaatcttta ttttaaaatt aataaattat tttgctctca gtaacaatta
      421 cttctgaggc tactttttat atctaaatta aaaaatggct aaaattaatg ctgtaatcac
      481 aggagttgga ggatatgttc cagattatat ccttaccaat gatgaattaa gtaccatgat
      541 ggacacaagt gatgaatgga ttaccagtcg tgtgggaatt aaagaacgtc atgttttaaa
      601 agataaggat ttgggaactt catttatggc agtaaaagcc attgaagatt tatttcaaaa
      661 aacaggcaca aaacctgaag aaattgaaat gataatatgt gctacagcaa ctcctgatta
      721 tattttacct tcgtgtgcct caattatcgc tgaaaaagtt ggaataaaaa atgccatagc
      781 ttttgattta gaggctgcct gtgcaggatt cattgtagct cttactaccg cagctgcttt
      841 tattgaatca ggaagatata aaaaaattct ggcaatagga gcagaaaaat ttacttctct
      901 tacaaattat gaagatcgta ccactgcgcc tctttttggt gatgctgccg gggtagtcct
      961 ggtagaaccg acagaagagg aagtaggttt tattgactct gttcttttta ctgatggaat
     1021 tggtttttct catttaggca caaatggtgg catatcaact catccccctt ctcatgaaac
     1081 agtagataaa aaaatgttta ccatttatca agaaggtcaa tatgtattta aacatgcagt
     1141 ttccgacatg tctgacgccg tagtaaaaat aatggaaaga aatcatctta caaaagacga
     1201 tgttaattgg ttaatccctc atcaagcaaa cttacgaatt attgatgcta ttgctcagcg
     1261 agtaggttta gattatgaga aagttattat caatattaat aaatatggta atacttcagc
     1321 ggctagtatt ccgttatgtc tttgggaata tgaaaataaa tttaaaaaag gagataatat
     1381 tattcttact gcttttggtg caggctttac atggggcgca gtgtatttaa aatggggcta
     1441 tagtaaacaa gaataaaata aacaaagctt ttttaaaaag aatatccaaa aagggtattc
     1501 tttttttatt cattttttgt atgtaaaatc cgattttctg caattttgca aaattcttca
     1561 tttatttcaa atccaataaa gttccttttt aacattttgc aagcaactgc tgttgttcct
     1621 gaaccaataa aaggatcgag aatcaaatca cctacattac tacttatttc aataaatgga
     1681 atgcacactt ttaaaggttt ttgagtctta tgtctggtac gttctttccc catacagata
     1741 ggtgcttcaa taaaattatg catttcattt actgatttat agttccaggt ttttggagta
     1801 ccttttgaaa aatgtacaag catttccatg ctgtttgaaa acattttacg gcttgaaata
     1861 gcagggaaag ggtttgtttt atgccagtgt ataactttcc tgaattgaaa cccaacttgt
     1921 tccaccagac gattgaaata ggaaacaaaa tcatcaccac accacaaata accacttgct
     1981 ccttctttgc ttactcgata acattccgct aaaaccctcc tcaaaaaatc caaataatct
     2041 ttttctgaat caaaatgatc aaaatcaaat tcagtaacta aatctttatg atttttaagc
     2101 ccttgtatat tttgagcacg atattgatag taagggggat cagtaaaaat taaatcaatg
     2161 gtattttctg gaatttgttt taagccatcc agacaggaaa tattatatat tttattgata
     2221 tcaagcaaaa tatgtaggta attggagttt ttatttttga tataaaatct ttattttttg
     2281 aaatacaaat cgagcaatat ttttgctccc ataaaggaac tgatttcgtt ggcaagtact
     2341 tttttctcca cttcaattaa ttttttctga atttcttcat tttggtaaaa attaacttta
     2401 agttgttcat caatagtttc atacatccag tattttgatt ggttgtttcg tttatattca
     2461 aaatagccat ttgctttagt aaattgaaca tatctttcaa tcatattcca cacttcatct
     2521 ataccttttc gttcgagtgc cgaacaagta agcacttcag gtgaccagcc agattctgtt
     2581 ggaggaaata agcgaagagc gttttgatgc tgaattttag caatttgagc tttatcaatg
     2641 ttgttcccgt cacatttatt tatcacgata ccatcggcca tttccataat gcctcttttt
     2701 attccctgca attcatcacc agctcctgca atctgtatca ataaaaagaa gtcaaccata
     2761 gaatgaacgg ctgtctctga ttgacccaca cctactgtct ctacaaaaat agtatcgaaa
     2821 ccagcagctt cacaaagaat aatgctttca cgcgtttttc ttgcaactcc tcctaatgtc
     2881 cctgctgacg gcgaaggacg aataaaagca ttctttgaag cagacaattc ttccattcgc
     2941 gttttatctc ccaatatgct tcctttgctt ctttcactgc tgggatctat tgccagaact
     3001 gccaatttat gtcctgaacg taccaaatac attcccaatg tttcaataaa agtactttta
     3061 cctgcccctg gaactcctgt aattcctact cgaattgatt tgccagcata tggtaaacat
     3121 ttttcaatta tttcctga
//
LOCUS       DAOTKS010000184         3138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_25533_length_3138_cov_4.657152, whole genome shotgun sequence.
ACCESSION   DAOTKS010000184 DAOTKS010000000
VERSION     DAOTKS010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25533_length_3138_cov_4.657152"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="P5236_04440"
     CDS             <1..194
                     /locus_tag="P5236_04440"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRU63643.1"
                     /translation="FIKELRIKRAAQFLRTGEYTVSEVTYMVGMNDARYFSKCFKKIY
                     GETPSEYKKRYETENKSSE"
     gene            complement(265..2856)
                     /gene="clpB"
                     /locus_tag="P5236_04445"
     CDS             complement(265..2856)
                     /gene="clpB"
                     /locus_tag="P5236_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HRU63644.1"
                     /translation="MNFDNFTIKSQEAIQDAIELVQSKGQQAIETPHLLKAILRTGED
                     VVQYLFGKLGVNTNALNLALDKMIDAYPRVSGAEPYLSRETNAVLQKAIDITSKSGDQ
                     FVSLESILLALVTEKNAVTRMLNDAGVNERTLSEAINTLRKGRKVTSETAEETYNALN
                     KYAINLNEQARAGKLDPVIGRDEEIRRVLQILSRRTKNNPILIGEPGTGKTAIVEGLA
                     QRIVRGDVPENLKTKQIFSLDMGALIAGAKYKGEFEERLKAVINEVVNSNGDVILFID
                     EIHTLVGAGKSEGAMDAANILKPALARGELRAIGATTLDEYQKYFEKDRALERRFQIV
                     MVDEPDVASSISILRGLKERYENHHKVRIKDDALIAAVELSHRYITDRFLPDKAIDLI
                     DEAAAKLRMEIDSVPEEIDVIERNIKQLEIEREAIKRENDEKKLEQLNQEIANLKEKS
                     QALRAKWSSEKALIDKIQQAKIEIEDLKFQAERAEREGDYGKVAEIRYGKIKALEDAI
                     AKYQEELAKIQGSNPMIKEEVDSEDIAEIVSKWTGIPVTKMLQSEKEKLLHLEEELHK
                     RVVGQDDAIQAVADAIRRSRAGLQDPKRPIGSFIFLGTTGVGKTELAKALAEYLFNDE
                     NMMTRIDMSEYQEKFSVTRLIGSPPGYVGYEEGGQLTEAIRRKPYSVVLFDEIEKAHP
                     DVFNILLQVLDDGRLTDNKGRIVNFKNTIIIMTSNIGSSLIRENFEKLNEYNHDEIIE
                     KTRNEVFELLKQTIRPEFLNRIDEIIMFKPLNRPQIEEIVRLQLKNIQEMLKQNGIQL
                     EATDKAIKLIADLGYDPQFGARPVKRVIQKLILNDLSKQIIAGAIQSNQTVIIDAVDD
                     TIVFRNK"
BASE COUNT          938 a          615 c          448 g         1137 t
ORIGIN      
        1 aatttataaa agaattacgt ataaaacgag ctgcccaatt tctcagaaca ggtgaatata
       61 cagtttcaga ggtaacttat atggtaggga tgaatgacgc tcgatatttc agcaagtgtt
      121 tcaaaaaaat ctatggagaa acaccttcgg aatataaaaa aagatatgaa acagaaaata
      181 aaagttctga atagttaaaa aagtaaggac tcttcataaa aattcaattt ttacaaagag
      241 tctttacttt aaatttccgt ttgtttattt attcctgaaa actattgtat catcaacagc
      301 atctattatc actgtctgat tactttgaat tgcacctgca ataatttgtt tcgacaaatc
      361 attaagaatc agcttttgaa tgactcgttt aacaggacgt gctccaaact gtggatcgta
      421 acctaaatca gcaatcaatt taattgcctt gtctgtggct tctaattgaa taccattttg
      481 tttgagcatt tcctgaatat tcttcagttg caaccgtacg atttcttcaa tttgaggccg
      541 attcagtggt ttaaacatga tgatttcatc aatcctgttt aaaaattcag gacgaatggt
      601 ttgttttaat aattcaaata cttcatttct tgttttttca ataatttcat catgattata
      661 ttcatttaat ttctcaaaat tttctcgaat taaagaggat ccaatgttag aagtcatgat
      721 gataatagta tttttaaaat tcacaattct tcccttattg tcagtcaatc tcccatcatc
      781 caatacttga agtaaaatat taaatacatc aggatgagct ttttcaattt catcaaaaag
      841 taccacggaa taaggtttac gacgaattgc ttctgtcaat tgtccccctt cttcgtaacc
      901 cacatagcca ggaggcgaac ctattaatcg tgtaacagag aatttttcct gatattcact
      961 catgtcaatt cgggtcatca tgttttcatc gttgaacaag tattcagcca aagcttttgc
     1021 caattcggtt tttccaacgc cagtcgtacc gagaaaaata aatgagccaa tgggacgttt
     1081 aggatcctgc agaccggctc tactgcgacg gatggcatct gctactgcct gaatggcatc
     1141 atcttgacca acaactcgtt tgtggagttc ttcttccaaa tgtaataact tttctttttc
     1201 actttgaagc atttttgtca caggtatgcc tgtccatttg cttacaattt cagcaatgtc
     1261 ttcgctgtca acctcctctt ttatcattgg gttcgagcct tgtatctttg ccagttcctc
     1321 ttggtactta gctattgcat cttccagagc tttgattttt ccgtaacgga tttcagctac
     1381 tttgccataa tcaccttctc tttcagcacg ttctgcctga aatttcagat cttctatctc
     1441 gattttggct tgttgaattt tatcaatcaa agctttctcg cttgaccatt ttgctcgtaa
     1501 agcttgactt ttttctttca aattagcaat ttcctgattc aattgttcta atttcttttc
     1561 atcattttcc cttttaatag cctctcgttc aatttccagt tgttttatat ttctttcaat
     1621 aacatcaatc tcttcaggaa cagaatcaat ttccattctt aattttgcag cggcttcatc
     1681 aattaagtca atggctttat caggcaaaaa acgatcagta atgtaacgat gcgaaagttc
     1741 aacagcagct attagagcat catcttttat tcgaacttta tgatgatttt catatctttc
     1801 tttcaaacca cgtaaaatag aaattgaact tgccacgtca ggttcgtcaa ccattactat
     1861 ttggaaacga cgttctaacg ctctgtcttt ttcaaaatat ttttgatatt catctaatgt
     1921 agtagcacca atagctcgca gctctcctcg agctaaagca ggttttaaaa tattggcagc
     1981 atccattgca ccttcacttt tgcctgcccc aaccaacgta tggatttcat caataaacag
     2041 aataacatca ccattggaat ttactacttc attgataacg gcttttaacc gttcttcaaa
     2101 ttcaccctta tatttagctc cagcaatcaa ggcacccata tccagtgaaa atatttgttt
     2161 tgttttcaaa ttttcaggca catcacctct cacaattctt tgagccagac cttcaacaat
     2221 agccgttttt cctgttcctg gttcgccaat taaaattgga ttatttttgg tacggcgact
     2281 caaaatctgt aaaactcgtc gaatctcttc atccctgcca ataacaggat caagttttcc
     2341 tgcacgtgcc tgttcgttca aattaattgc atatttattt aatgcattat aagtttcttc
     2401 tgctgtttca gaagttactt tccttccttt tcgcaaagta ttgatggctt cagaaagcgt
     2461 tctttcattc actcctgcat cattcaacat tcgcgtaact gcattttttt cagttacgag
     2521 tgccaataaa atagattcca gagacacaaa ctgatcacca gatttagaag taatatctat
     2581 tgccttttgt agtactgcat ttgtctcacg acttaaatat ggttcagcac ctgacactct
     2641 tgggtaagca tcgatcattt tatctaaggc taaattcaag gcatttgtat tcactcctaa
     2701 tttcccaaat aaatattgga caacatcttc tcccgttcgg agaattgcct ttaacaagtg
     2761 tggagtttca atagcttgtt gacccttgct ctgtaccaat tcaatagcat cttgaatggc
     2821 ttcctgagat ttaattgtaa aattatcaaa attcataact ttaatttttt atattaaaat
     2881 aaaatctttt taaattgtct tttattaata aaaattgaaa aataaattta aaattttatt
     2941 ttattctcta caaattattt accaattaaa tatttcggta agaatgtctt ttttgtcgaa
     3001 atagtataaa gaagcactga caatatttca gtaaaaatta ttcccttaat ataaagttga
     3061 aattgtaact tggagatgac aatttttgca attccttttt tttctgataa aaaaaaatta
     3121 tttttgtaga atgaaaat
//
LOCUS       DAOTKS010000185         3135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_25564_length_3135_cov_3.882792, whole genome shotgun sequence.
ACCESSION   DAOTKS010000185 DAOTKS010000000
VERSION     DAOTKS010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25564_length_3135_cov_3.882792"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="P5236_04450"
     CDS             <1..638
                     /locus_tag="P5236_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HRU63645.1"
                     /translation="VDDNSPDGTAAIVKNLQKEFPEKLFLLERGGKLGLGTAYIEGFK
                     WALDRDYKYIYEMDADFSHNPNDLPKLYQACANEGVDLAIGSRYLTGVNVVNWPLGRV
                     LMSYMASIYVRLVTGLKITDTTAGFKCYRREVLETIELDKIRFKGYGFQIEMKFKAYK
                     SGFTLKEIPIIFINRELGTSKMSGGIFSEAFLGVIKLKIQSFFYHYPQKKK"
     gene            674..2023
                     /locus_tag="P5236_04455"
     CDS             674..2023
                     /locus_tag="P5236_04455"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRU63646.1"
                     /translation="MNKFQLIRNATIVNEGETFIGSVLIKDKFIQAIYANEADIQLKE
                     PFDEIDAEGYWLLPGVIDEHVHFREPGLTHKGDLYTESKAAVAGGVTSFMDMPNTIPP
                     TITIEALEQKFQLASEKSLANYSFYLGVTNDNFEELKKADIHRVCGIKIFLGSSTGNL
                     LVDNELTLERIFAEIPFLIAVHCEDEQMIQQHIRYYQNLIGEELPIFYHPLIRDTDVC
                     YRATTKAVKLAEKYQSRLHVLHVSTEKEISLFSSSIPLNEKKITAETCIHYLWFSDED
                     YEQLGNSIKCNPAIKSKDNRSALRKAINDNRLDVVATDHAPHLWNEKEGSCLKAASGA
                     PFVQHSLTAMLQLVKEGIFTLEKVVEKMCHAPAVIYKIQKRGFIRPGYYADLVLINPS
                     CSWQVSPDNLLCKCGWSSFTGTKFDSKVVKTWVNGHKVYDEGYFDESIKGEPLIFDN"
     gene            2037..3065
                     /locus_tag="P5236_04460"
     CDS             2037..3065
                     /locus_tag="P5236_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HRU63647.1"
                     /translation="MMNRKKIYSLIALLAIGLIIFFIFSKNKQGKQYIHNEGRIFGTY
                     YHITYQHPQQIDLQKKIEERLHEFENSLSIFNKQSIISRINQNDTSVVTDAYFEKMFQ
                     VAQKISKETNGAFDITVSPLVNVWGFGTNDTNRIQAPEVKNILPYVGYWKIHLENHKL
                     IKDDPRIMLDASAIAKGYSADVIGQLLKENQCENYLVEIGGEIVCKGLNPKGEKWRIG
                     IDKPIEDSTNMINEIQTIIHISNKALATSGNYRQFYYKKERKFSHIIDPRTGFPVNNN
                     VLSTTVIAPTCIEADAYATAFMVLGVDSTLELCKKLPEIDSYLIYVDNQGKDQVIYTE
                     GFKKYLFQ"
BASE COUNT         1112 a          438 c          571 g         1014 t
ORIGIN      
        1 ttgttgatga taattcaccc gatgggacag ccgcaattgt aaaaaatcta caaaaagaat
       61 ttcctgaaaa actttttctt ctcgaacgag gaggaaaatt aggacttgga accgcttata
      121 tagagggttt taaatgggca ttagatagag attataaata tatttatgaa atggatgccg
      181 atttttcgca taatccaaat gatttaccga aattatatca agcttgtgct aatgaaggag
      241 ttgatttagc aattggttct cgttatttaa ctggtgtaaa tgtagttaac tggcctttag
      301 gaagagtatt aatgtcttat atggcttcta tttatgtaag attagtaaca ggtctgaaaa
      361 ttacagatac tactgcaggt tttaaatgtt atcgtagaga agtgcttgaa acaattgaac
      421 tggataaaat tcgtttcaaa ggttatggtt tccagatcga aatgaaattc aaagcttata
      481 aaagtggttt tacattaaaa gaaataccca ttatctttat aaatagagaa ttaggtactt
      541 ccaaaatgag tggcggaatt tttagtgagg cttttttagg ggtgatcaaa ttaaaaattc
      601 aaagtttctt ttatcattat ccacaaaaga aaaaataatt ttcgaatcct tccccaagca
      661 atgtttataa tttatgaata aatttcaatt aatccgaaat gcgactattg ttaatgaagg
      721 agaaactttt ataggttcgg tacttataaa agataaattt attcaagcaa tatatgcgaa
      781 tgaagcagat attcaattga aagaaccttt cgatgaaatc gatgcagaag gttattggct
      841 tttgcctggt gtaattgatg aacacgttca ttttcgtgaa ccgggcttaa ctcataaagg
      901 cgatttatat accgaaagca aggctgcggt agcaggtggt gttaccagtt ttatggatat
      961 gcctaacact attccaccca caataaccat tgaggcttta gagcaaaaat ttcaacttgc
     1021 atcagaaaaa tcacttgcaa attattcttt ttatcttgga gttaccaatg ataatttcga
     1081 agaacttaaa aaagcagaca ttcatcgtgt ttgtggaatc aaaatttttt tggggtcatc
     1141 aacaggaaat ttgctggttg acaatgaatt gactttagaa cgaatttttg ctgaaattcc
     1201 ctttctgatt gctgttcatt gtgaagatga acaaatgatt cagcaacata ttcgttatta
     1261 tcaaaatttg ataggagagg agctccctat tttttatcat ccgttaattc gagatacaga
     1321 tgtttgttat cgagctacaa cgaaagctgt aaaattagca gaaaaatatc aaagtcgttt
     1381 gcatgtgttg catgtttcta ctgaaaagga aatatcattg ttctcttcca gtattccatt
     1441 gaatgagaag aaaataacag cagaaacctg cattcattat ttatggtttt ctgatgagga
     1501 ttatgaacaa ttaggaaata gtatcaaatg taatcctgct attaaaagca aagataatcg
     1561 ttcagcattg agaaaagcta taaatgataa tcgattggat gtggtggcga ctgatcatgc
     1621 acctcatttg tggaacgaaa aggaaggttc ttgtttaaaa gctgcttctg gagcaccgtt
     1681 tgtacaacat tcgctaaccg ctatgttaca attggtaaaa gaaggtattt ttacactgga
     1741 aaaagtagta gaaaaaatgt gccatgctcc tgctgttatt tataaaattc aaaaacgtgg
     1801 ttttatcaga ccaggttatt atgctgattt agtgctgatt aatccatcgt gttcctggca
     1861 agtttcgcct gataatttgc tatgcaagtg tggctggtca tcttttacag gaactaaatt
     1921 tgattcaaaa gttgttaaaa cctgggtcaa tggtcacaaa gtttatgacg aaggatattt
     1981 tgacgaaagt ataaaaggcg aaccgttaat ttttgataat tgaaaacagt gtatttatga
     2041 tgaacagaaa aaaaatctat tctttaattg ccctattggc tatcggatta ataatatttt
     2101 tcatctttag caaaaacaag cagggaaaac aatatattca taatgaagga aggatttttg
     2161 gaacttatta tcatattact taccaacatc cacaacaaat tgatttacag aaaaaaatag
     2221 aagagcggct acatgaattt gaaaattcac tttccatttt caataaacaa tctattattt
     2281 cacgtattaa tcaaaatgat acttcggtag ttactgatgc ctattttgaa aaaatgtttc
     2341 aggtcgctca aaaaatttca aaagaaacta acggagcttt tgatattact gtatcacctt
     2401 tagtaaatgt atggggattt ggaacaaatg ataccaaccg aattcaagca cctgaggtaa
     2461 agaatatttt gccatatgtt ggttattgga aaattcatct ggaaaatcat aaattaataa
     2521 aggatgatcc tcgaattatg ctggatgcga gtgccatagc caaaggttat tctgctgatg
     2581 taattggtca attattaaaa gaaaatcaat gtgagaatta tctggtagaa attggaggtg
     2641 aaatagtttg taaaggactg aatcctaaag gtgaaaaatg gcgtattgga attgataaac
     2701 caattgagga cagtacaaac atgataaacg aaattcaaac tattattcat atttccaata
     2761 aagctttggc gacttcaggc aattaccgcc aattttatta caaaaaagaa agaaaattct
     2821 ctcatattat cgatccaaga acaggttttc cagtgaataa caatgtgtta agcaccactg
     2881 tgatagcacc aacctgcatt gaagcagatg cttatgccac tgctttcatg gtattaggtg
     2941 tggatagtac tttagaactt tgtaaaaaat tacctgaaat agacagttac ttgatatatg
     3001 tggataatca agggaaagat caagtaattt atactgaagg gtttaaaaaa tatctttttc
     3061 aataaaatgt aaaaaacaaa gaattttatt taaatagaaa atttaacttt tttgttttag
     3121 ataaaacagt taatt
//
LOCUS       DAOTKS010000186         3135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_25565_length_3135_cov_3.550325, whole genome shotgun sequence.
ACCESSION   DAOTKS010000186 DAOTKS010000000
VERSION     DAOTKS010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25565_length_3135_cov_3.550325"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..298)
                     /locus_tag="P5236_04465"
     CDS             complement(53..298)
                     /locus_tag="P5236_04465"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HRU63648.1"
                     /translation="MGHPMQYLFNLEKGRILQEYQIIYITQRYGVFQNKHKVYRIYPG
                     SVIILFPEEWHRYRPLKTLDEKVSTSDLMVIFSSVTS"
     gene            complement(706..2313)
                     /locus_tag="P5236_04470"
     CDS             complement(706..2313)
                     /locus_tag="P5236_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRU63649.1"
                     /translation="MNKNKLLAPFIVFVAIICGLLLGNILARRASNLSVDTLFSAIKP
                     HSKIDELFSIINSKYVDTLDMHQLTEATMVDIVSKLDPHSVYISAEDVSDVNSELEGH
                     FSGIGVQFNIQNDTIMIISVISGGPSEKAGLLAGDRIVEVNDTLFVGKNITNEKVMKK
                     LRGPAGTKVKVGVRRYGTPEILYYEIIRGEIPVNSVDIGYMIQPQIGFIRVSKFAETT
                     YTEFLNTIASLKRQGAKKFIVDLRENSGGYMDQTIQMVNEFLPANRLIVYAQGRAYKR
                     FEAFSDGKGSCIHEPLVVLINEFSASASEIFAGAIQDNDRGTIVGRRSFGKGLVQQQF
                     TLSDGSAVRLTVARYYTPSGRCIQKPYELGKVDQYEKDFLNRLIHPDINYSDSTQKID
                     SLRYKTVGGRIVYGGGGIMPDVYVPLDTTGYTPYLNKVINYGYLYQYAFLYTDQNRGK
                     LSTFKNWQQMEKYLNTQPLLENFVRFALTKGVPVNKKEINISKDIILTQLKAYISRNI
                     LGDEGFYPLLYKNDKTVRKALEVLSRQ"
     gene            complement(2392..2811)
                     /locus_tag="P5236_04475"
     CDS             complement(2392..2811)
                     /locus_tag="P5236_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HRU63650.1"
                     /translation="MTEKQRLLDQRYMRMARIWGENSYCKRRQVGALLVKDKMIISDG
                     YNGTPSGFENDCEDEENNSKPYVLHAEANAITKVARSHNSSEGATLYVTASPCMECAK
                     LIIQAGIKRVVYGEQYRIADGIELLKRAGIEVVFIDV"
     gene            complement(2847..>3135)
                     /locus_tag="P5236_04480"
     CDS             complement(2847..>3135)
                     /locus_tag="P5236_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan synthetase"
                     /protein_id="HRU63651.1"
                     /translation="TEDFLPQYYGSMSEADIALVYYDPEVIKQKQLQEFSPTDVKNAF
                     GEKVEAFNRKEDLLEKLHELNYENTVVLFMSSGNFSGINLEELTNELIKNQ"
BASE COUNT         1019 a          560 c          448 g         1108 t
ORIGIN      
        1 ttttcggttg ccatattaaa gcgggtgaag cgaacgaatt agtattagca tatcaagaag
       61 ttacagaaga gaagataacc atcaagtccg atgtagagac tttttcatcc agtgttttta
      121 atggtctgta acgatgccac tcttctggaa ataaaataat aacactacca ggatagattc
      181 tatatacttt gtgtttgttt tgaaaaacac catatctttg tgtaatataa atgatttgat
      241 attcttgcaa aattcgacct ttttctaaat taaacaaata ttgcataggg tgtcccattt
      301 gaatggggaa ttctgaattg gatggaattt cgaagtccca acaactgtta agtataaact
      361 taatttttgg tcttgttttg aaaaagtagt gtatttaaat gattgtattt tcattgggat
      421 taatattttc ttttactctc aattttcttc aagataaatt gataatgtaa agtaaattat
      481 tatatagatt ctaatttgta aatacacgaa tttttttgaa agggataaaa cctttcgaaa
      541 tattatatgc agatcttgat taaaaagaat aaccatttgt gcaatcaatt tttaaacttt
      601 atataaaaac aacaaaaaaa tcattgtaca caataacata ccaagaatta ttgcacactt
      661 ttttggatat tctttttgaa taaaagaaca aaatttcttt aaaatttatt gcctgcttaa
      721 tacttcaaga gcttttctga cagttttatc atttttatat agcaaaggat aaaaaccttc
      781 atctcctaga atgttccttg aaatgtaagc ttttaattgt gttaagataa tatcttttga
      841 gatgttaatt tcttttttat tgacaggtac accttttgtt aaagcaaaac gaacaaaatt
      901 ctctaagagt ggttgagtat ttaaatattt ttccatttgt tgccagtttt tgaaggttga
      961 taattttccg cgattctgat ctgtatataa aaaggcatac tggtaaagat atccataatt
     1021 aatcacttta ttcagataag gcgtatagcc agtagtatcc aatggcacgt aaacatcagg
     1081 catgatacca ccacccccgt aaacaattct accaccaaca gttttgtatc tcaaactgtc
     1141 tattttttga gtactgtctg aataattaat atctggatga ataaggcgat ttaaaaaatc
     1201 tttctcatat tggtcaacct ttcctaattc ataaggtttc tgtatacaac gcccagaagg
     1261 agtataatat cttgccactg ttaaacgaac agcagaacca tccgaaagag taaattgttg
     1321 ctgtacaagt cctttaccaa aagaacgtct tcctacaatt gttcctctat cattatcttg
     1381 aatggcacca gcaaaaattt cactggctga agctgaaaat tcattgatta atactactaa
     1441 tggctcatga atacaactac ctttaccatc cgagaatgct tcaaaacgtt tataagctct
     1501 cccttgtgca taaacgatta atctgttggc aggcagaaat tcattaacca tttgaatagt
     1561 ttgatccatg taaccgccac tattttcacg taagtccaca ataaattttt tagctccttg
     1621 tcgctttaaa ctggcaatag tatttaaaaa ttccgtatat gtagtttcag caaatttact
     1681 tacccgaata aaaccaattt gaggctgaat catgtaacca atatctaccg aatttacagg
     1741 tatctcacct cttattatct cataataaag gatttctggt gttccataac gacgaacgcc
     1801 gacttttact tttgtaccag cgggaccacg tagtttcttc ataacttttt cattggtgat
     1861 attctttcca acaaaaagtg tatcgttcac ttctactatt ctgtcgcctg ccaataaacc
     1921 tgctttttca gaaggtccgc cgctaataac agaaataatc ataatcgtat cattctgaat
     1981 attaaattgt acaccaattc cactgaaatg cccttcgagc tcactattaa catctgaaac
     2041 atcttcggca gaaatatata cggaatgtgg atccagtttt gaaacaatgt caaccatcgt
     2101 tgcttctgtt aactgatgca tgtccagtgt atccacatat tttgaattaa taatggaaaa
     2161 caattcatca atttttgaat gtggttttat tgcagagaac aaagtatcaa ctgataaatt
     2221 agaagctctt cttgctaaaa tatttcctaa aagcaaacca caaattattg ctacaaaaac
     2281 tataaaaggc gctaataatt tatttttgtt catatatagt tactttatta tttttattaa
     2341 attgttagtt atcaaaaatt taaagatatt tattaagaat ttctgagaaa attatacgtc
     2401 aataaacact acctcaattc ctgcccgttt aagtagttca atgccatcgg caattcgata
     2461 ttgttctcca taaactactc gtttgatgcc tgcctggata atcaattttg cacattccat
     2521 acagggtgag gcagttacat aaagtgttgc tccttcgcta ctgttatggg aacgggcaac
     2581 ttttgtaata gcatttgctt cggcatggag aacatacggt tttgaattgt tttcttcgtc
     2641 ttcacaatca ttttcaaaac ccgatggagt tccattataa ccatcagaaa taatcatttt
     2701 atcttttact aatagagctc ccacctgtct tcttttgcaa tatgaatttt ccccccagat
     2761 acgagccatt cgcatatagc gttgatccag caatcgttgt ttttcagtca taatcttttc
     2821 tacatcttat ttttaaatga agtaaatcat tgatttttaa tcagctcatt tgttaattct
     2881 tctaaattta taccagaaaa atttcctgaa gacataaaca gaacgactgt attctcataa
     2941 ttcaattcat gcaatttttc cagcaaatct tctttccggt taaaagcttc tactttttct
     3001 ccgaaagcat ttttcacatc agttggagaa aattcctgta actgtttttg cttgataact
     3061 tctggatcat aatataccag tgcaatatct gcttcactca tacttccata gtactgaggt
     3121 aaaaaatctt cggtt
//
LOCUS       DAOTKS010000187         3118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_25717_length_3118_cov_3.953967, whole genome shotgun sequence.
ACCESSION   DAOTKS010000187 DAOTKS010000000
VERSION     DAOTKS010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25717_length_3118_cov_3.953967"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="P5236_04485"
     CDS             <1..590
                     /locus_tag="P5236_04485"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016270226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRU63652.1"
                     /translation="KASSDHFVSAYYHTYGLPVIISHCSNNYGANQFPEKLIPLCINN
                     IKNKKSIPIYGKGENVRDWLWVNDHAAAIDLIFHKGKIGEIYNIGGNNEWKNIDLIKK
                     LCEIMDRKLGQVEGESAKLITFIKDRPGHDLRYAIDSSKLQKELGWKPSVNFEEGLEK
                     TVEWYLENEEWLNNVINGDYLKYYEEQYQNQENEK"
     assembly_gap    950..959
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1010..2788
                     /locus_tag="P5236_04490"
     CDS             1010..2788
                     /locus_tag="P5236_04490"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HRU63653.1"
                     /translation="MEKVLKRISLLRQLMHENKIKAYMIPSTDPHASEYVADYWKERE
                     WISGFDGSAGTVVITMDKAALWTDSRYFLQAEEQLKGTGIELMREGLTETPGIITWII
                     SQLNNNEKVGINPQMISCNAYSSMKEELKMNNLDLVSVDLISKIWTDRPSLPIDPFYE
                     YDIKFAGKSATEKISSVRQQMKQINAEVFLLSALDEIAWLFNIRGNDVAYNPVVIAYG
                     VIELDRAILFVNPLKVTTESSKYLKSEGIIVEPYEEIYSYLKQLAPQQSIWMDGAKLN
                     QSVYEAIPAHCKIINKTSPVIYMKSIKNETEIAGIKQAMIKDGIALIRFFKWLEENVE
                     KNQLTELSVSEQLHHFRNEQENFKGESFETIVGYASHGAIVHYQPTPETNFSIKPKNL
                     LLIDSGGQYLEGTTDITRTISLGNPTNQQKKDFTLVLKGHIELAQVKFPKGTRGSQID
                     IMARQFLWKEGINYGHGTGHGVGHFLCVHEGPQNIRIDENPTALEPGMLISNEPGVYR
                     ANEYGIRTENLVLVTFAEKTDFGEFYQFETLSLCPIDKALIDRNILTENEINWLNNYH
                     QKVFEILSPFLNEEERKWFQQKCLPL"
     gene            2810..>3118
                     /locus_tag="P5236_04495"
     CDS             2810..>3118
                     /locus_tag="P5236_04495"
                     /inference="COORDINATES: protein motif:HMM:NF026169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HRU63654.1"
                     /translation="MISDLNDSFLGKKVPIVENYDPSLLFKIPRSINREKYNISSENL
                     PFVGADAWNCYEVSFLTNNGLPISRVMKLIYSANSTYLVESKSLKLYLNSFNMEKFGE
                     T"
BASE COUNT         1105 a          450 c          547 g         1006 t
ORIGIN      
        1 caaaagcaag ttctgatcat tttgtttctg cttattatca tacatatggt ttaccagtaa
       61 tcatttctca ctgttccaac aattatggag caaatcaatt ccctgaaaaa ttgattcctc
      121 tttgcatcaa caacatcaaa aacaaaaaat caattccaat ttacggaaaa ggtgaaaatg
      181 tgcgtgattg gctttgggtt aacgatcatg ctgctgccat tgatctcatt tttcataaag
      241 gaaaaatcgg agaaatttac aacattggag gaaataacga atggaaaaat attgatttaa
      301 taaaaaaatt atgtgaaata atggaccgta aattggggca agttgaagga gaatctgcca
      361 aattaattac cttcataaaa gaccgtccag gacacgattt gcgttatgca attgattctt
      421 ctaaactaca aaaagaattg gggtggaaac cttctgtaaa ttttgaagaa ggattggaaa
      481 agacagtgga atggtatctg gaaaatgaag aatggctcaa caatgtcatc aacggcgatt
      541 atttgaaata ttatgaggaa caatatcaaa atcaagaaaa tgagaaatga atttatttct
      601 tttgaaaatg attttttgaa tctcctctta aaatctcatt tttcaaaagg aatattaatt
      661 tttttgttgt cttgatatgt cgttggataa tagtaaccta attgtctcag ctatacaatt
      721 cctcttggga aatgtcttgc agtttttgtc tcaaaagaga agttctcata ttttaaaaat
      781 taaagtagcg atatttgcca ttctctgcct tcctggtaat aattcgcagt actctgaata
      841 agttcaccag aagaagaaaa taaatttaat tgcttttcgg tttctgactt tctaaacatt
      901 tttatcttat ttatttcact aaattactga aatataataa tatatgaaan nnnnnnnnnt
      961 actttttata acattcaatt aaaatttatt ttttcaaatt atttttgcta tggaaaaggt
     1021 attaaaacga atttctttgc ttcgtcaatt aatgcatgaa aataaaatta aggcttatat
     1081 gatacccagc accgatccac atgcaagtga atatgtggct gattattgga aagaacgtga
     1141 atggataagt ggttttgatg gctctgctgg cacagtggta attacaatgg acaaagctgc
     1201 cttatggacg gattcgcgtt attttttaca agcggaagaa caattaaaag gaacaggaat
     1261 tgaattgatg agagaaggtt taaccgaaac gccgggaata ataacctgga taatttctca
     1321 acttaataat aatgaaaaag ttggaataaa cccgcaaatg atttcctgca atgcttattc
     1381 atcaatgaag gaagaattaa aaatgaataa tcttgacctg gtttcggtgg atttaataag
     1441 taaaatttgg acagataggc cttctcttcc tatagatcct ttttatgaat atgatataaa
     1501 gttcgcagga aaatcagcca cggagaaaat aagttcagtt agacagcaaa tgaaacaaat
     1561 caatgccgaa gttttccttc tttcagctct ggatgaaatt gcatggttgt tcaatattcg
     1621 agggaatgat gttgcttaca atcctgtagt aatagcttat ggagtaatag aattagatcg
     1681 tgccattttg tttgttaacc ctttgaaagt aactaccgaa tcatcaaagt atttaaaaag
     1741 tgaaggaata attgtcgaac cttatgaaga aatttattct tatttgaaac aattagctcc
     1801 tcaacaatct atctggatgg atggtgcaaa attgaatcaa tctgtttatg aggctattcc
     1861 tgctcattgt aaaataatca ataaaacttc tcctgtaatt tacatgaaaa gtataaaaaa
     1921 tgaaacagaa atagcaggca tcaagcaagc tatgataaaa gatgggattg ctttgattag
     1981 attctttaaa tggttagaag aaaatgttga aaaaaatcaa cttactgagc tttctgtttc
     2041 agagcaatta catcatttta gaaatgaaca ggaaaatttt aaaggtgaaa gttttgaaac
     2101 catagtaggt tatgcttctc atggagctat tgtgcattat caaccaactc cagaaacaaa
     2161 tttttctata aaaccgaaaa atttattgct cattgattcc ggaggacaat atttagaagg
     2221 aactacagat attacacgaa ctatttcttt aggaaatcct acgaatcaac aaaaaaagga
     2281 ttttacgctg gttttgaaag gacatattga attggctcaa gtaaagtttc caaaaggtac
     2341 acgaggttca caaatagata ttatggctcg acagtttctc tggaaagaag gaataaatta
     2401 cggacatgga acaggtcatg gagtgggaca ctttttgtgt gtgcacgaag gacctcaaaa
     2461 tatacgaata gatgaaaatc ctaccgcatt ggagcctgga atgttaattt caaacgaacc
     2521 aggcgtttat cgagccaatg aatatggcat tcgaactgaa aatttagtac ttgttacttt
     2581 tgcagaaaaa accgattttg gcgaatttta ccagttcgaa acattgagcc tttgtccaat
     2641 tgataaggct ttaattgaca gaaatatact tactgaaaat gaaattaatt ggttgaataa
     2701 ttatcatcaa aaagtgtttg aaattttatc tccttttctt aatgaagaag aaagaaaatg
     2761 gttccaacaa aaatgtcttc ctttataata cactgatttg tataaattta tgatatcaga
     2821 tttgaatgac tcttttttag ggaagaaagt tcccattgtg gaaaattatg atccttcttt
     2881 gttgttcaaa attcctcgtt ccatcaatcg ggagaaatat aatatttcca gtgaaaattt
     2941 accttttgtc ggagcagatg catggaattg ttatgaagtt tcctttttga caaataatgg
     3001 tttaccaatt tctcgtgtaa tgaaattgat ctattcagcc aacagtactt atttggtcga
     3061 atctaagtca cttaaattgt atttgaactc ctttaacatg gaaaaatttg gtgaaacg
//
LOCUS       DAOTKS010000188         3115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_25743_length_3115_cov_3.381373, whole genome shotgun sequence.
ACCESSION   DAOTKS010000188 DAOTKS010000000
VERSION     DAOTKS010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25743_length_3115_cov_3.381373"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..1137)
                     /locus_tag="P5236_04500"
     CDS             complement(103..1137)
                     /locus_tag="P5236_04500"
                     /inference="COORDINATES: protein motif:HMM:NF018123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HRU63655.1"
                     /translation="MNTFPFIMLAILSGFLNGSYAVPIKLTQKWSWENIWLWFAIYGL
                     LLVPWIFALVFVPDIGKVLADSSTGSTCITFLCGLGWGIGSVAFVLALHVIGFSLGYT
                     LMMGLIAVIGALVPLLVNNPSALFTKGGIFILIALIVAIVGIILCGAAGKIREEKNFN
                     QNGQQINDKSKIKKGLFLCVISGFFSPMINFAFEFGAPLKNSALKQLGNDSNIFYATS
                     VIWAVALSGGFIAFLFYSSYLLAKNKTISNFSLKGTHSYFFWTFLMGITFYSSLLCYG
                     LAASGLGDAGTTTGWIIFTAGAIIIANFWGIITSEWKGVPKRGIRKMVLGSILLISTV
                     FLVSYGNSLL"
     gene            complement(1139..2107)
                     /locus_tag="P5236_04505"
     CDS             complement(1139..2107)
                     /locus_tag="P5236_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018919666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRU63656.1"
                     /translation="MYKYSVILGNLGNTCDRFCKGYKDNPSTEEMLAQAVQIPHVEGI
                     ELVGTWDIRPDNVKDMKKRMEDYSKTCVSIIPDLFTSKEYGKGTLSNTDPGIRQKSLD
                     YLRQMSEIAQEMNCGIINLWMAQDGYDYLLTADYDQERKWLQEGITKLASEFPDLRFA
                     LEYKPKEPRNFSYQARVADTILAAKETGCANVGICIDTGHSFVAGENVAEAVVLAKRA
                     GNLLFHMHFNDNYGFWDDDMIVGTVHNAVYIDLLYNLKKTGYSGWLSMDQYPYREDAT
                     DAIAESILWVKKFEQIVENNYEEIGSLVKLNDATKTSRFLRKFLVI"
     gene            complement(2204..>3115)
                     /locus_tag="P5236_04510"
     CDS             complement(2204..>3115)
                     /locus_tag="P5236_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019967495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRU63657.1"
                     /translation="RHINNTNGLNISPYPYANIPALWNCGESPTQELVDCFEMTNGAL
                     PVTYNDATHTNVTINPDAAAVGYSESTGGNPYLNRDARFYVDVLYNGANYGVPTNCIE
                     PVIVESYVGGAHGFNDIISMETKTSCTGYYSRKDKDVKFWGPNQSGLGPEIHWVFFRL
                     AEFYLNKAEALCELNDLEGAKDALNVIRQRARQPKIEDVPGFSNTKEFLRERIRNERR
                     IELCLEGWRFHDQRRWKILDKTNRLVTGMRITVADRDEHGKPISFNYNRVKVRDYQSY
                     TNKYLVMPIPINDAKKMTGMTQPEAWQ"
BASE COUNT          998 a          637 c          449 g         1031 t
ORIGIN      
        1 aattcttaac tttaccctat acttttaatt aaccttcagt actgattagt tacatatttt
       61 aatattcaat tttgctcaat tatttatttt aaataaattt attcataata agctgtttcc
      121 gtaacttaca agaaaaacag tacttatcag caagatcgaa cctaatacca ttttccttat
      181 tcctcttttt ggaacccctt tccattcaga agtaatgatt ccccaaaaat tagctattat
      241 aatggcacca gcagtaaata tgatccatcc ggtcgttgtt cctgcatctc ccagaccgga
      301 agctgccaat ccataacaaa gtaaagaact gtaaaaagta atacccatca aaaaagtcca
      361 gaagaaataa gagtgtgtgc ccttcagtga aaaattgcta atcgttttat tttttgcaag
      421 taaatatgaa gaataaaaca aaaaagcaat aaatcctcct gaaagagcca cagcccaaat
      481 aaccgaagta gcataaaaaa tatttgaatc atttccaagt tgctttaaag cactattttt
      541 taaaggagct ccaaattcaa atgcaaaatt gatcatcgga ctaaaaaaac ctgaaataac
      601 acacagaaaa agacctttct taatttttga tttatcattt atttgctgtc cattctggtt
      661 aaaatttttc tcttctctta tttttccagc tgctccacaa agaattatcc caactattgc
      721 tactattaag gcaataagta taaaaattcc tccttttgtg aatagtgctg aagggttatt
      781 taccaataaa ggaaccaagg ctccaattac agcaataaga cccatcatta atgtatatcc
      841 cagagaaaaa cctattacat gcagagcaag aacaaaggca actgaaccta ttccccatcc
      901 gagtccacat aaaaaggtta tacacgttga acctgttgac gaatctgcta aaacttttcc
      961 tatatcagga acaaacacaa gtgcaaatat ccatggaaca agcaataatc cataaatagc
     1021 aaaccataac catatatttt cccatgacca cttttgggtt aatttaatcg gaacggcata
     1081 acttccattt aaaaatccgg aaagtattgc caacattata aatggaaaag tattcatatt
     1141 agattactaa aaattttctc aaaaaacgcg aagtttttgt cgcatcattc aattttacta
     1201 agcttcctat ttcctcataa ttattttcca caatttgttc aaattttttc acccataaaa
     1261 tgctttcagc aatcgcatca gtagcatctt ctctataagg atattgatcc atagacaacc
     1321 atccggaata tcctgttttt ttcaaattat ataacaaatc tatataaacg gcattatgaa
     1381 cagtgccaac aatcatatcg tcgtcccaaa atccgtaatt atcattaaaa tgcatgtgaa
     1441 aaagtaaatt gcctgcacgt tttgcaagta caactgcttc tgcaacattt tcaccagcca
     1501 caaaactatg tcccgtatct atacatattc ctacatttgc acaaccggtt tcctttgctg
     1561 ccagaatagt atcagctaca cgtgcctgat aagaaaaatt tcttggctcc ttaggtttgt
     1621 attccaacgc aaacctcaaa tcaggaaatt cgctcgccaa tttggtgatc ccttcctgta
     1681 gccatttccg ttcctgatca taatcggcag ttagcagata atcatagccg tcttgagcca
     1741 tccacaagtt aatgatgccg caattcattt cttgcgcaat ttcactcatt tggcgtaggt
     1801 aatctaatga tttttgacga attcccggat cggtatttga aagagttccc tttccgtatt
     1861 cttttgatgt aaataagtca ggaattatag agacgcaggt cttactgtaa tcttccatcc
     1921 gttttttcat gtcttttacg ttgtcaggtc gaatatccca ggtaccaacc agctcaattc
     1981 cttctacatg tggaatttgt actgcttggg caagcatttc ttcagttgat ggattgtctt
     2041 tatatccttt acaaaagcga tcacatgtat tgcctaaatt acctaaaatt acagaatatt
     2101 tatacatatt actattattt gtttataagt taattttatt taattatctt tttaaatgat
     2161 gttaccgagg aatcttttaa ttcctcggta actaatcata tttttattgc catgcttcag
     2221 gttgagtcat accggtcatt tttttagcat cattaattgg aatcggcatt actaaatatt
     2281 tgttagtata tgattgataa tctctaacct ttacgcgatt ataattaaag gaaattggtt
     2341 tgccatgttc atctctatct gcaactgtta tcctcatgcc agtaaccagc ctattcgttt
     2401 tatcaagtat tttccatctg cgctggtcat ggaatcgcca tccttcaaga catagttcta
     2461 ttcgtcgttc atttctaata cgttcacgca gaaattcttt agtattggaa aaacctggaa
     2521 catcttctat tttcggttgg cgagctcttt gacgaataac gttcaaggca tctttggcac
     2581 cttccaaatc gtttaactca cataaagctt ctgctttatt gaggtaaaat tctgcaagtc
     2641 ggaagaaaac ccaatgaatt tcaggaccta gtcctgattg attcggaccc caaaatttta
     2701 catctttatc tttgcgacta taataacctg tacaagaggt ttttgtttcc atgctaatta
     2761 tatcgttaaa accatgtgca ccgcctacat aactttctac gataactggt tctatacagt
     2821 tagtcggaac tccataatta gccccattat acaagacatc aacatagaat cgggcatccc
     2881 tgttgagata tggattgcca cctgttgatt cactatatcc tacagcagca gcatctggat
     2941 taatagtaac attagtatga gttgcatcat tgtaagtaac tggtaatgca ccatttgtca
     3001 tttcaaaaca gtctactaat tcttgtgtag gtgattcacc acaattccaa agtgcaggaa
     3061 tgttagcata cggataaggt gagatattta acccattggt gttattaata tgtct
//
LOCUS       DAOTKS010000189         3111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_25783_length_3111_cov_3.490183, whole genome shotgun sequence.
ACCESSION   DAOTKS010000189 DAOTKS010000000
VERSION     DAOTKS010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25783_length_3111_cov_3.490183"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="P5236_04515"
     CDS             complement(<1..265)
                     /locus_tag="P5236_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HRU63658.1"
                     /translation="MNVDELPDHVVYSQKVIEFVTVAAETCLFLEHASEIDKHDFVDK
                     SLKLLPLLYLKASLIEIPEPVYDEEPERFVTEEDYQFVRQQVEQ"
     gene            572..>1889
                     /locus_tag="P5236_04520"
     CDS             572..>1889
                     /locus_tag="P5236_04520"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRU63659.1"
                     /translation="MKKLTFKTSLLLALFFLWGSGIKAETYYVDFESLAKTSYASGTV
                     TLGAYDWNFTGACAGNLDSDWKVGSVSARLAGTTKASSNSETSKIEMLSNKAGGIGNI
                     SFQYRHYGSDAQIPWLVQWSSDGSTWTTIQEITGTEQVQTFSYDLNQDNARIRIIANG
                     FTTSDANGKRLNVDELILTDNSGTPVPTITVTETTLEGFSYEVGKGPSAEKSFQVSGS
                     NLTGNITLTPSDNLEISTGTGENFVATSPINLPPTEGVVAATSIYARLKAGLPAGNYT
                     ETITANSTGAVTKTITCKGNVFEPVGTVPNVIITEVYGGGGNSGAELKNDFIELYNTT
                     NTPVNIAGWSLQYYAATGTGKAADANVFVIPDGKSIPAYTHFLIQCAAGAGGTQDLPT
                     PDATCELTLGGTAGKVILYTTSEPQSITTTDITSITGNPYFKDYVPY"
     assembly_gap    1890..1989
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1990..2439
                     /locus_tag="P5236_04525"
     CDS             <1990..2439
                     /locus_tag="P5236_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63660.1"
                     /translation="TDITSITGNPYFKDYVPYGTTAVPVWGTPMSSNTSNTTSAARKI
                     ISDATPRIYQYTGNIGNDFEVATPTPQNTEWTSGIKNVFENLNVYVADGKIHINTTTV
                     GEQIEVYNTLGQRLVNVLSTGEENIISVNAKGVLLVKCGNKVGKVIL"
     gene            2854..>3111
                     /locus_tag="P5236_04530"
     CDS             2854..>3111
                     /locus_tag="P5236_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63661.1"
                     /translation="MKKLTFLKSLLLGILFSFSANMYALSVGDIAIIAVNTDAPKTFT
                     FVALADIPANTTISFTDNAWNATTSTWRTGEGTIQWSHTAAV"
BASE COUNT          986 a          536 c          532 g          957 t
ORIGIN      
        1 gttgttccac ttgttgacgg acaaattgat aatcttcttc ggttacaaat ctttcgggct
       61 cttcgtcata aacaggttca ggaatttcaa ttagcgatgc tttcaaatat agcaaaggca
      121 ataatttcaa tgatttatca acaaaatcat gcttgtcgat ttcagaagca tgctctaaaa
      181 aaagacaggt ttcggctgca acggtcacaa attcaatcac tttttgacta taaacaacat
      241 gatcgggtaa ttcatcaaca ttcataaaac agtttttaat gattatttca aaacaaattt
      301 acaagataat tttcaattta taacaggttg ccattataaa taaaataaaa ataagtcaat
      361 agtttggagt tctcgaagtt gcgggatgcg agtttgaaac catttgttca ataaaaaaat
      421 aaattgaata attatttctt tttttacaaa aattatttta catttgaagt tccttttatt
      481 tcaattgaaa ttttcatatt caataatttt caacaataag aaaatttctt gtagttttca
      541 tagaatgtca aatatttaaa acaaaaaaat tatgaaaaaa cttactttta aaacaagtct
      601 gctattagct ctattttttc tctggggtag tggcataaaa gctgaaacgt actatgttga
      661 ttttgaaagt ttagctaaaa cttcttatgc atcaggaacc gtaactttgg gagcttacga
      721 ttggaatttt actggcgctt gtgcaggtaa tctagacagt gattggaaag tgggaagtgt
      781 ctctgctcga ttggctggta ccactaaagc ttccagcaat tcagaaactt caaaaattga
      841 aatgctctcc aacaaagcag gaggcattgg gaatatttct ttccaatatc ggcattatgg
      901 aagcgatgca caaattccct ggctggttca gtggagctcc gatggcagta cttggacaac
      961 cattcaagaa attactggaa ccgaacaagt tcaaactttc tcttatgatt tgaatcaaga
     1021 caatgcacgt atccgaatta ttgccaatgg ttttactact tctgacgcta acggaaaacg
     1081 tctgaatgtg gatgaattga tattgactga caactcaggc actcctgtcc ccaccattac
     1141 cgtaaccgaa acaacgttgg aaggattttc ttatgaagta gggaaaggac cttcagcaga
     1201 aaaatcattc caggtaagcg gcagcaatct tacgggtaat attaccttaa cgccatcaga
     1261 caatcttgaa atttcaacag gaacgggaga aaattttgtt gctacttcgc ctatcaacct
     1321 gcctccaaca gagggagttg ttgctgctac ttctatttat gctcgcttaa aagcaggact
     1381 acctgcagga aattacactg aaaccattac ggctaattct acaggagcag taactaaaac
     1441 catcacctgc aaaggaaatg tttttgaacc tgtaggtact gttcctaatg tgattattac
     1501 agaagtgtat ggcggtggag gtaattcagg tgctgagttg aaaaatgatt ttattgaatt
     1561 gtataatact accaatacac ccgtaaacat agcaggttgg agtctccaat attatgcagc
     1621 aacaggtaca ggcaaagcag cagatgcaaa tgtatttgtg atacccgatg gcaaatcaat
     1681 tcctgcttac actcattttc tgatacaatg tgcagcaggt gctggtggaa cgcaagattt
     1741 gcctactcct gatgctactt gtgagttaac tcttggtggc acagcaggca aagtaatact
     1801 ctatactact tccgaacctc aatctattac cactactgat attacttcca ttacaggaaa
     1861 tccatatttt aaagattatg ttccttatgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnna ctgatattac ttccattaca ggaaatccat attttaaaga ttatgttcct
     2041 tatgggacta ctgctgtacc agtttgggga acacccatga gtagtaatac ttctaacact
     2101 acttcggctg caagaaaaat catttctgat gctactcctc gtatctatca atatactgga
     2161 aatattggaa atgattttga ggtcgctacc cccactcctc aaaatacaga atggacatca
     2221 ggtataaaaa atgtttttga aaacttgaac gtgtatgtag ccgacgggaa aatccacatc
     2281 aataccacaa cagttggaga acaaattgaa gtgtacaata ccttaggtca acgattggtt
     2341 aatgttcttt ccacaggaga agaaaatatt atttctgtca acgccaaagg agttcttttg
     2401 gtaaaatgtg gcaacaaagt tgggaaagtt attttataac tttataagca ggtaactttt
     2461 tcatggtagg taaaaagctg ctcgtttttc gggcagcttt tttcttgctt actgcaatca
     2521 tcttctattg ccgaaccatt gtatcgggtg gcatacagga aattaaatac cggaatattg
     2581 aatgaatttt ttgcttttcc agagcaagga acagacaaaa gtttacacca acaaaaataa
     2641 aataaaaaaa tactgaaaaa aattgaatat ttatttgcca agtataaaaa tatgctttac
     2701 ttttattgct cattttcatt ctcgacagag aaaacaagtc ttttttaata gctttttcac
     2761 agaaaagtaa agacacagtt ggttttcttt tttaattttg taaaaattct tttttaaaat
     2821 aacacaatgt ttcactttta aaaatttacc gttatgaaaa aacttacttt tttgaaatcg
     2881 ttgctgctgg gcatattgtt ttctttcagt gctaatatgt atgccctaag tgtaggtgat
     2941 attgctatca ttgcagtgaa caccgatgct ccgaaaacat ttacttttgt tgctttagca
     3001 gatatacctg ctaacactac tattagtttt actgacaatg catggaatgc tactacttct
     3061 acgtggagaa ctggtgaagg cactattcaa tggtctcata cagctgcggt a
//
LOCUS       DAOTKS010000190         3106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_25830_length_3106_cov_3.188463, whole genome shotgun sequence.
ACCESSION   DAOTKS010000190 DAOTKS010000000
VERSION     DAOTKS010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25830_length_3106_cov_3.188463"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..1141)
                     /locus_tag="P5236_04535"
     CDS             complement(50..1141)
                     /locus_tag="P5236_04535"
                     /inference="COORDINATES: protein motif:HMM:NF015940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HRU63662.1"
                     /translation="MKYLFQLNHPAHYHLFKNVIHQLKKDGHQVLILARPKDILIDLL
                     ENEDFDILKIEKGQTLFQKIKLRKQTEKYILNYTEKHKPDLIIGTGDFSYVTKKQGIP
                     SIFCGEDDANLNLFLFLWALTVYGDFSAILAPEGVNNSIWNNKTIFYKGYHKLAYLHP
                     NQFTPDKKVVEKYLSTDKPYFILRFAKLNAYHDVNAKGIDSVIAQKLIKILHPQGDIY
                     ITSERELEPEFEQFRLKIDPLDIHHVLFFSKLYIGDSQSMAVEAAMLGVPSVRFNNFA
                     GKISVLEELEHKYGLTFGIKTSQPKKLFEKVKELLLMENLSEEFQNRRQTMLADKIDV
                     TAFLVWFIENFPESAKIMKETPDYQYNFK"
     gene            complement(1145..2146)
                     /locus_tag="P5236_04540"
     CDS             complement(1145..2146)
                     /locus_tag="P5236_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HRU63663.1"
                     /translation="MDAYIKAISYALPEKIVTNEDLVKDFPEWTVEKIAGKVGIEERR
                     VAAENETAADLAVKAAEQLFSEYNIDKNSIDFILFCTQSPDYFLPTSACIIQNRLELR
                     KEIGALDYNLGCSGYIYGLSLAKGLISANIAKNILLLTGETYNKSIHPQDKGNRTIFG
                     DAASASLISDEGFAKIGEFSLGTDGSGAENLIVKTGGFRFPDALNDLHFDENGNLISS
                     DYLFMNGEEIFNFTSDAVPVLVDDVLKKNHAKLNEIDLFVFHQANKYMMNYLRKLIGI
                     DAEKFYVYLQKVGNTVSSTIPIALYHAKKGNKLHGNVLLAGFGVGYSWGGVILKIID"
     gene            complement(2223..2456)
                     /locus_tag="P5236_04545"
     CDS             complement(2223..2456)
                     /locus_tag="P5236_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRU63664.1"
                     /translation="MNQEEFIQNFASQFDETDTSIFTMETRFKELDEWSSLISLAILN
                     MLAKKFGVKLTPEEMKRSQTLQELYDLVLSKKQ"
     gene            complement(2465..2980)
                     /locus_tag="P5236_04550"
     CDS             complement(2465..2980)
                     /locus_tag="P5236_04550"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRU63665.1"
                     /translation="MEQGFKTEKGIPYYEDTQKRSIGQKLKKIKNTLLGNMAYSCPIK
                     KLRILFHRWRGVYIGKNVYIGIHCFFDNLYPEYIYILDNASVNADCMILAHFNPMKRF
                     SQILSARVAPVIINEGAIIAVRSTILPGVKIGKNAIVSAGSVVEKDVPDYTLVKGNPA
                     KIITEFKYLME"
BASE COUNT         1039 a          541 c          426 g         1100 t
ORIGIN      
        1 acaataaatt tttttctata aaaacatttg ccaatttcca taaaattcat tatttgaaat
       61 tatattgata atcaggagtt tccttcatga tttttgcact ttccggaaaa ttttcgataa
      121 accagaccaa aaaggctgta acatcaattt tatctgctaa cattgtttga cgacggtttt
      181 gaaattcctc gcttagattt tccatcaaca ataattcttt tactttttca aaaagttttt
      241 tcggttggct ggtttttatt ccaaatgtaa gaccgtattt gtgttctaat tcttccagaa
      301 cactgatttt ccccgcaaaa ttgttaaaac gtactgaagg aacaccaagc attgctgctt
      361 caacagccat actttggcta tcgccgatat aaagtttgga aaaaaacaat acgtgatgaa
      421 tgtcgagagg atcaattttc aagcgaaatt gttcaaattc aggttcaagt tctctctcgg
      481 aagtaatata aatatcacct tgtgggtgaa gaattttaat taatttttgt gctatcactg
      541 aatcaattcc ttttgcattt acatcgtggt aagcgttcaa ctttgcaaat cgcagaataa
      601 aataaggttt gtcggtagac agatattttt caaccacttt cttgtcaggt gtaaactgat
      661 tcgggtgcaa atatgctaac ttatgataac ctttataaaa aatagtctta ttattccaga
      721 ttgaattatt tacgccttcg ggagccaaga tagcagaaaa atcgccatat acagttaatg
      781 cccataaaaa taaaaacaga tttaaatttg catcgtcttc tccacaaaaa attgaaggaa
      841 ttccttgttt ttttgtaaca tacgaaaaat ctcctgtacc aataatcaaa tcaggtttgt
      901 gtttttctgt atagtttaaa atatattttt ctgtttgttt tcttaacttg attttttgaa
      961 aaagagtttg tcctttttca atttttaata tatcaaaatc ttcattttcc aataaatcaa
     1021 ttaaaatatc ttttggacgt gcaagaatca atacctgatg tccatccttt tttaattgat
     1081 gaataacatt tttaaacaaa tgataatgtg caggatgatt taattggaaa agatatttca
     1141 ttttttaatc gataattttt aatataacac caccccacga ataaccaaca ccaaaaccag
     1201 ccaaaagaac atttccatgc aatttgtttc ctttttttgc gtgatatagg gcaattggga
     1261 tggtggacga aactgtatta cctacttttt gaagataaac gtaaaatttt tcagcatcta
     1321 ttccaatcag ttttcgtaaa taattcatca tatatttatt agcttgatga aatacaaata
     1381 aatctatttc attcaatttt gcatgattct ttttgagcac gtcatcaacc aagacaggaa
     1441 cggcatcaga agtaaaatta aatatctctt ccccattcat aaacaaataa tcagaggaaa
     1501 ttaaattccc gttttcatca aaatgtaaat cattcaaagc atctgggaat ctaaatccgc
     1561 cagttttaac gattaagttt tctgccccac taccatctgt gcccaaagaa aattcaccta
     1621 tttttgcaaa accctcatca gaaataagag aggctgatgc tgcatcccca aaaatagtac
     1681 gattgccttt atcttgagga tgaatacttt tattataagt ttcaccggtt aaaagcagta
     1741 tgttttttgc aatattagca gaaattaacc cttttgctaa tgacaagcca taaatataac
     1801 ctgaacatcc caaattgtaa tccaatgctc caatttcttt tcgcaattca agccgatttt
     1861 gaataatgca agctgaagtt ggcaaaaaat aatcaggact ttgagtacag aatagaataa
     1921 aatcaatgga gttcttgtca atattatatt ctgaaaataa ttgttcagca gcttttacag
     1981 ccaaatcagc agcagtttcg ttttctgcgg ccactcttcg ctcttctata ccaactttcc
     2041 ctgcaatttt ttcaacagtc cattcaggaa aatcttttac cagatcttca tttgttacaa
     2101 ttttttcagg taaagcataa gaaatagctt taatataggc atccatccga ttaaatttat
     2161 tgaatttgaa tgatataagt tttaatgttc aatataaaat tgtttaaatg aaaaaaattt
     2221 ttttattgtt ttttggataa aaccaaatca tacaattctt gaagcgtttg agatcgcttc
     2281 atttcttctg gagtcaattt tacgccaaat tttttggcta acatattaag tattgccaaa
     2341 gaaataagcg aagaccattc atcaagctct ttaaaacgtg tttccatcgt aaaaattgag
     2401 gtatcagttt catcaaactg acttgcaaag ttttgaataa attcttcttg attcatcata
     2461 tattttattc cattaaatat ttaaattctg taattatttt ggccgggttg cctttaacca
     2521 aagtataatc tggtacatct ttttctacaa cagaaccagc agaaacaatg gcattttttc
     2581 caatttttac accaggcaaa atcgttgatc taacagctat aattgctcct tcgttaataa
     2641 tcacaggagc aactcgagct gataaaattt gagaaaatcg tttcatggga ttaaaatgtg
     2701 ctaagatcat gcagtctgca ttaacactgg cgttatctaa aatataaata tactctggat
     2761 ataaattatc aaaaaaacaa tggataccaa tataaacgtt tttgccaata tatacacctc
     2821 tccagcgatg aaataaaatt cttaactttt ttatagggca agaatatgcc atattaccta
     2881 aaagtgtatt tttgattttt ttaagcttct gcccgatact gcgtttttga gtatcttcat
     2941 aatacgggat tcccttttca gttttaaaac cttgttccat tatttttaaa tttacttaaa
     3001 aatctgattg cattttcccc aaggagatag aaacatttgt tgtttacatg caaaaatctt
     3061 ccatattcaa ccgtttcgcc accaaatttt tctttgaatt cacgaa
//
LOCUS       DAOTKS010000191         3104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_25854_length_3104_cov_4.760905, whole genome shotgun sequence.
ACCESSION   DAOTKS010000191 DAOTKS010000000
VERSION     DAOTKS010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25854_length_3104_cov_4.760905"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            654..1196
                     /gene="hpt"
                     /locus_tag="P5236_04555"
     CDS             654..1196
                     /gene="hpt"
                     /locus_tag="P5236_04555"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006283624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HRU63666.1"
                     /translation="MNIIQIEDKKFKLYIPEKEILKSIQEIAEKINDDLVDKNPLFIC
                     ILNGAFMFASDLMKAISIPCEITFIKLSSYHGTTTSGKIKEIIGLNEDITKRNIVVIE
                     DIIDTGFTMEFIINSLKSKGANEIQIAALLLKPDALQRDLKIDYAGIKIPNDFIIGYG
                     LDYNGYGRNLRDIYTLISEQ"
     gene            1235..1825
                     /locus_tag="P5236_04560"
     CDS             1235..1825
                     /locus_tag="P5236_04560"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRU63667.1"
                     /translation="MLNIILFGAPGSGKGTQSKLIAQKYHLKHISTGELLRTEMKKQS
                     ELGKIAENYILKGNLLPDEIIMDILIKNIDDDLKNYNGILFDGFPRTLPQAEMLEKIL
                     HELNTEITLLIDLKVNEEELIKRLLKRAEIEIRNDDKPDIIKKRLEIYAQNAEPINEF
                     YKKLNKYIAIDGMGTVEEVFSRIEKVINSKLNEAKN"
     gene            1868..3019
                     /gene="obgE"
                     /locus_tag="P5236_04565"
     CDS             1868..3019
                     /gene="obgE"
                     /locus_tag="P5236_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014298352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRU63668.1"
                     /translation="MATSNFVDYVKIYCRSGKGGAGSTHFRHEKYLPKGGPDGGDGGR
                     GGHIILRGNKNYWTLLHLKYARHIFAEDGENGSKNRSFGKSGQDKIIEVPCGTVVYDG
                     DTGEFICDITQDGEEVVLLEGGRGGLGNWHFRTATMQAPRFSQPGEPRKEKTIILQLK
                     VLADVGLVGFPNAGKSTLLSVVSAAKPKIADYPFTTLVPQLGIVSYRDNQSFVMADIP
                     GIIEGAAEGRGLGLRFLRHIERNSILLFMIPADSKNIQEEYYILLSELEKYNPQLLEK
                     ERILAISKSDLLDKNSLKDLKNNLSMDIPSVFISSITGEGINELKNLLWTELNKPSNQ
                     LLEISHQPLSIAHHKIEIEKEEKDDDISFSEENEEENFNQFKGIGWDDQ"
BASE COUNT         1139 a          464 c          524 g          977 t
ORIGIN      
        1 tatgaattgt tttatacaaa aaaactagaa taaaacatta ggattttaaa ccaaaagaaa
       61 ttgttaggtt taagttatca ggatttagtt agtagtcagg agtttggaat tcaagaacgg
      121 taaggttcta attgtgagaa aaatgagtaa ttataatttg gcttgacagg tcagtaataa
      181 aaaaattctg tgttttctcg atatgactct gtgaaatttt aatttttatt actcagaaat
      241 acacaaaaat ttacccagca agacataaag atttatttct tgctcttgtg cagtcaaata
      301 aaaccctaaa taattgaaac cgcaaaactc atatttcgta ccagtatttc gtgatgcaca
      361 aactcgcaag tcctaaactt ttttaacacc aaacagttga tcaattattt ttataaatcg
      421 atgtctttct gtttatttga gctaaaaaat ttattccgcc aaataatttg attttcttaa
      481 gaaaataaca ttaattcata ttatcttctt gcttccttct aattataaat gtttttagta
      541 ttagtgtttt ttcttatttt tgcaaattag atgaaaatta tgcacttttt gtgcatttta
      601 tgcaaaataa aataaccatt attatatata aaatggaaga tttaccgcac tctatgaata
      661 tcattcaaat cgaagacaaa aaatttaaac tttatattcc tgaaaaagaa atattaaaat
      721 caattcagga aattgcagaa aaaatcaatg atgacctggt agataaaaat ccacttttta
      781 tctgtatctt gaatggggca ttcatgtttg ctagcgattt gatgaaagca atttctatcc
      841 cttgcgaaat tacttttata aaattatctt cttatcatgg cacaactact tctgggaaaa
      901 taaaagaaat cattggctta aatgaagata tcacgaaacg aaatatagta gtaattgaag
      961 atattattga cacaggcttt acaatggaat tcattataaa ttctctcaaa tcaaaaggag
     1021 ctaacgaaat tcaaatagct gctttacttt taaaacctga tgctttacaa cgagacctaa
     1081 aaattgatta tgcaggtata aaaattccca atgatttcat cataggctat ggattagatt
     1141 ataatggtta tggaaggaat ttaagagata tttatacttt aatttctgaa caatagccaa
     1201 ataacataaa aattctatat aagacaaaat taaaatgctg aatatcattt tgttcggagc
     1261 tccaggaagt ggaaaaggaa cacaaagtaa actcattgcc cagaaatacc atctcaaaca
     1321 tatatcaaca ggagaattac tacgcactga aatgaaaaag caatcagagc ttggtaaaat
     1381 cgctgaaaat tatattttaa aaggcaattt acttcctgac gaaataataa tggatatttt
     1441 aatcaaaaat attgatgacg atttaaaaaa ctacaatgga attctttttg atggctttcc
     1501 tcgaacactt ccgcaggcag aaatgcttga aaaaatactc catgaactaa atactgaaat
     1561 aacgcttttg attgacttga aagttaatga agaggaatta ataaaacgcc ttttaaaacg
     1621 agcagaaatt gaaataagaa acgatgacaa accggatata atcaaaaaaa gattggaaat
     1681 ttatgctcaa aatgcagagc caattaatga attttataag aaattaaaca agtatattgc
     1741 tattgatgga atgggaactg tggaagaagt tttttctcgt attgaaaaag taatcaactc
     1801 aaaattaaat gaggctaaaa actaaattac cttaattttg ttatcaagtt aggtataaaa
     1861 aattcagatg gcaacttcca attttgttga ttacgtaaaa atctattgtc gttctggcaa
     1921 aggaggcgca ggttctactc acttccgtca tgaaaaatat ctaccaaaag ggggtcctga
     1981 tggcggtgat ggtggacgtg gtggacatat tattttaaga ggaaataaaa attattggac
     2041 tttgctacat ctaaaatatg ctcgtcacat ttttgctgaa gatggtgaaa atggcagcaa
     2101 aaatagaagt tttggcaaaa gtggtcaaga taaaatcatt gaagttcctt gtggcacagt
     2161 agtttacgat ggtgatacag gagaatttat ttgcgatatt acacaagatg gggaagaagt
     2221 tgttttgctg gaaggtggac gcggtggact tgggaactgg catttcagaa ccgctacgat
     2281 gcaagctccg cgtttctctc aaccgggtga gccacgaaaa gaaaaaacta tcattttaca
     2341 actcaaagtg ctggccgatg tagggctggt tggtttcccc aatgctggaa aatcaacctt
     2401 gttatcagtt gtttcggctg ctaaaccaaa aattgccgat tatcctttca ctacattggt
     2461 tccacaactc ggaatcgttt cctatcgtga caatcaatca ttcgttatgg ctgatattcc
     2521 tggcatcatt gaaggagcgg cagaaggtcg tggactggga cttcgttttc ttcgacatat
     2581 cgaacgcaat tctattcttc tgtttatgat tccagcggat agcaaaaata ttcaggaaga
     2641 atattatatt ttactcagcg aattggaaaa atacaatcca caacttcttg aaaaagaacg
     2701 cattcttgcc atttcaaaaa gtgatttgct ggataaaaat tcactgaagg atttgaaaaa
     2761 caatctttcg atggatattc cttcggtatt tatctcttct atcacaggcg aaggtatcaa
     2821 cgaattgaaa aatttactgt ggacagaact taataaacca tccaatcaac tgctggaaat
     2881 ttctcaccaa cctctttcta ttgctcatca taaaattgag atagaaaaag aagaaaagga
     2941 cgatgatatt tctttttcag aagagaatga agaagaaaat tttaatcaat ttaaaggaat
     3001 tggctgggac gatcaataat tttttcactg aatgaattga tgtatgtttt tgaagaaaaa
     3061 aactatcctt ccgttaaaaa actgaaaaat tatcttgaaa gctt
//
LOCUS       DAOTKS010000192         3079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_26132_length_3079_cov_4.130291, whole genome shotgun sequence.
ACCESSION   DAOTKS010000192 DAOTKS010000000
VERSION     DAOTKS010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26132_length_3079_cov_4.130291"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..857
                     /locus_tag="P5236_04570"
     CDS             117..857
                     /locus_tag="P5236_04570"
                     /inference="COORDINATES: protein motif:HMM:NF026067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4465 domain-containing protein"
                     /protein_id="HRU63669.1"
                     /translation="MKKSKWFLFALLGMSFIFSSCEKENPFETTNVTVDFEDVQLGNG
                     NYWNGSDGKGEFLANGFRFKNTYVSSEWGDFWSGFACSAMNDTITQGYANQYSTIAGS
                     GAFSSHQFAIAYDDSATILIPENELATYSAKSIMLTNTAYSYWDMKNGSAFSKKFAAG
                     DWFKVIIKGFWKGRETSSLEYFLADFRNGKSFISNKWKEVNISTLGKIDQLSFFFDSS
                     DKGAFGVNTPKYVCIDNLQFEKSLNNEE"
     gene            1222..2556
                     /locus_tag="P5236_04575"
     CDS             1222..2556
                     /locus_tag="P5236_04575"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HRU63670.1"
                     /translation="MDIKPYLKETGKLFNYHNWKNFFFSSPTRKKLTENFLSLSVLQG
                     LNYLLPLITLPYLVRVLGPEKYGLVAFAQAFIAYFGILTDYGFNLSATREISIQRENK
                     EKISAIFSSVMLIKFVFLILSFILMSVLVFSIHKFRNEWLLYFLTFGTVLGQVLFPIW
                     FFQGIERMKYITWLNIIAKTIFTVLVFVLIHQKTDYVYVPFLGALGTIISGVLALGII
                     RKHFKVNFHLPSKNEVIHQLREGWYIFISTVAISLYSISNTFILGLFASNTIVGYYSG
                     AEKIINAVQGLLSPVSQTIYPHISKIASESKERALKFLQKITILIGSSSFFLSLLIFL
                     LAEVVIKILLGGQYLESIKVLRILSFLPFIVGLSNIFGIQTMLTFDYKKAFSNILIIA
                     SLINITLAFILVPFFFHIGIAFAVVISETFVTIAMFVYLQRKGIRVLEGKYV"
     gene            2549..>3079
                     /locus_tag="P5236_04580"
     CDS             2549..>3079
                     /locus_tag="P5236_04580"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HRU63671.1"
                     /translation="MFDYIMVGAGFAGSILAERIANILNKKVLIIEKRNHTGGNCYDE
                     KDENGIMVHKYGPHLFHTNEKSVFDYLSNFTDWHPYVHKVLAFIDGKKVPIPFNLNTL
                     YMVFPKTFARKLEEKLINTFGFNSKIPVLELKKSTDKDLQMLADYVYEKLFLHYTLKQ
                     WGMKPEEINPEVSGRVP"
BASE COUNT          975 a          423 c          552 g         1129 t
ORIGIN      
        1 agaagtttga acttttcttt ttcattcttc cttttcgttt ctttattctc tttttactga
       61 gcagaaaagt tcggtgaact attttttaat attataaact ttaaaaaatg taaaaaatga
      121 aaaagagtaa atggttttta ttcgctttat taggaatgtc ttttattttt tcttcctgtg
      181 aaaaggaaaa tccgtttgaa acaacgaatg taacggtaga ttttgaagat gttcagttag
      241 gaaatggaaa ttactggaat ggttctgacg ggaaaggaga atttttagct aatggttttc
      301 gttttaaaaa cacttatgtc tccagtgaat ggggcgattt ctggagtggt tttgcttgtt
      361 ctgccatgaa tgacacaatt acacaaggtt atgcaaatca atacagtacc atagccggaa
      421 gtggagcttt tagttctcat cagtttgcaa ttgcttacga cgattctgca acaatattga
      481 ttcctgaaaa tgaactggca acttattctg ccaaaagcat tatgctgacc aacactgctt
      541 attcttattg ggatatgaaa aatggttctg ctttcagcaa gaaatttgct gcaggagatt
      601 ggttcaaagt aattatcaaa ggtttttgga aaggacgtga aacaagcagt ttggaatatt
      661 ttctggcaga ttttcgcaat ggcaaaagtt ttatttctaa taaatggaaa gaagtaaaca
      721 taagcacatt aggtaaaatt gaccagttaa gtttcttttt cgattcgagc gacaagggag
      781 cttttggtgt taatacgccc aaatatgttt gcatagataa tttacagttt gagaaaagtc
      841 taaataatga agaataaaat tttttagaaa ggtgaattta cttaattagt ttacctataa
      901 tgttttatga tttttgcagg gaaatttcac tctctgcaat ttttttattg ttttaatcaa
      961 aagaattcct cgaggcttat ctcgtggtta tccattagga aaaattaaaa taaacaaatt
     1021 tattttgttt gataatcaat tgtttataaa tcttttattt ttaagttaat aacatttcat
     1081 tagatatttt atttatattt gcaatggctt tgcgtcgcta aaaataatta aagagtaata
     1141 atgatttttg aagttttgag tttataaaat taaagaggaa taaaaaagaa atttgaaaaa
     1201 ctcaatttga aaatgtgaat aatggatata aaaccatatt tgaaagaaac aggaaaactt
     1261 ttcaattatc ataattggaa aaatttcttt tttagttctc ctacacgaaa aaaactaact
     1321 gaaaattttc tgtctctttc agttttgcaa ggtttgaatt atcttttgcc tttaattacg
     1381 ctgccttatc tcgtaagagt tcttggacct gagaaatacg gtttggtagc ttttgctcaa
     1441 gcatttatcg cttattttgg aattttaacg gattatggtt ttaacctgtc agccacgcga
     1501 gagatttcca ttcagcgaga aaacaaagaa aaaatttcag caatattcag ttccgtgatg
     1561 ttgattaaat ttgtttttct gattctttcg tttatcctga tgtctgtgct ggtcttttct
     1621 attcataagt tcagaaatga atggctatta tactttttga cttttggaac tgttttggga
     1681 caggtattgt tcccgatctg gttttttcaa ggcatagaaa ggatgaaata tattacctgg
     1741 ctaaatatta tagcaaaaac tatttttacc gtgctggttt ttgtactcat tcatcagaaa
     1801 accgattatg tttatgtacc atttttaggt gctttgggaa caattatcag tggcgttttg
     1861 gctcttggaa ttatcagaaa acattttaaa gtgaattttc atttaccatc aaaaaatgaa
     1921 gttattcatc agttaagaga aggttggtac atttttattt ctacggttgc aataagtttg
     1981 tattctattt ccaatacttt tattttgggt ttatttgcca gcaataccat tgtgggatat
     2041 tattctggag ctgaaaaaat aatcaatgct gttcaaggtt tgttgtcgcc tgtttcgcaa
     2101 accatttatc ctcatatcag taaaatagcc agcgagtcca aagaaagagc cttaaaattt
     2161 ttgcaaaaaa taactattct tattggcagt agcagctttt ttttgtctct tctaattttc
     2221 cttttagctg aagtggtaat aaaaatttta ttaggtggtc aatacctgga atcgataaag
     2281 gtgctgagaa ttttatcgtt tttgccattt attgtggggt tgagtaacat ttttggcatt
     2341 caaaccatgt tgactttcga ttataaaaaa gctttttcga atattttaat cattgccagt
     2401 ctgattaata tcacattggc atttatcctg gtgccttttt tcttccatat tggaattgct
     2461 ttcgctgtcg tgatttctga aacttttgta acaattgcta tgtttgtata tttgcaaaga
     2521 aaaggtatca gagttttgga gggaaaatat gtttgattac ataatggtag gcgcaggttt
     2581 tgcaggcagt atattggctg aaagaatagc caatatactg aacaagaaag ttttaataat
     2641 tgaaaaaaga aaccacacag gtggaaactg ttacgacgaa aaagacgaga atggtatcat
     2701 ggttcacaaa tatggtccac atctttttca tacgaatgaa aaatcggttt ttgattatct
     2761 ttccaatttt accgattggc atccgtatgt tcataaagtg ctggcattta ttgatgggaa
     2821 aaaagtgcca attcctttca atttgaatac actttacatg gtttttccta aaacttttgc
     2881 ccgaaaattg gaagaaaaac tgatcaatac ttttggtttc aactcaaaaa ttcctgttct
     2941 tgaacttaaa aagtcaactg ataaagattt gcagatgttg gcggattatg tttacgaaaa
     3001 attgtttttg cattataccc tcaaacaatg gggaatgaaa cctgaagaga ttaatccaga
     3061 agtaagtggt agagtgcct
//
LOCUS       DAOTKS010000193         3056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_26373_length_3056_cov_4.943352, whole genome shotgun sequence.
ACCESSION   DAOTKS010000193 DAOTKS010000000
VERSION     DAOTKS010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26373_length_3056_cov_4.943352"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3056)
                     /locus_tag="P5236_04585"
     CDS             complement(<1..>3056)
                     /locus_tag="P5236_04585"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HRU63672.1"
                     /translation="TNCYEWYLFDAVDFENIFYKNQEFRNNYENWDTKQKVDNTTVSL
                     YENIRNFIDKNVAELKATYFDLRDYEAALNSHDSENLSQLINLYKIFSPEHLLKKPFA
                     NDSNTLNKEFYNELLYILGLEEKSENNKIVIERRSKDRSEASLIENTINILKTRDKLK
                     GIEDITQFGQTEEEQLFSVALELCITWLNRILFLKLLEGQLIKYHNNDSNFAFLSPDK
                     VKEFDKLDELFFEVFAIKPAERTQKIQEQYAHIPYLNSSLFEMSLLEQQTISIANLKN
                     DQQLPIYNKSVLKIATNKKEINTLQYLLQFLSSYNFSSDSHAKIQEDNRTIINASVLG
                     LIFEKINGYKDGAFFTPGFITMFMSRETIRKAVVQKFREKENKEFNDFDDVKNYCRLH
                     HKKEDIERFNDHINSLKICDPAVGSGHFLVSALNELIAVKSELNILSYNGIIINWEVS
                     VDNDELTVTHKFNNQPYEYFLMDDEKPAPDAQEVQMTLFNEKRLLIENCLFGVDINPK
                     SVLICRLRLWIELLKNAYYKAPDFKELETLPNIDINIKCGDSLISRFNLTDKHYNLLP
                     AAQQKIRYIIQKYKQQVIIYKSTTDRATRHQTEQEIQRLKKQFMSVADPSDTDYKKWK
                     EKQNEYLNFMQQPISFEENAQKEWKNKLDELSKEVHELEEIYNEKLKTIYNRAFEWRF
                     EFPEVLDDDGNFVGFDVVIGNPPYIQLQKAYDDKRKFADLYQNQHYETFDRTGDIYCL
                     FYEKGITILKNEGLLCYISSNKWMRAGYGEKLRTFFLKYQPLLLVDLGAGVFESATVD
                     TNILLLQKSKNTAKTYLLQAVTIQKTNAQPIDIAQQIKEKAVILQNLTKEAWYIGNEA
                     EQQLKKKIERIGKPLKDWNVKIYRGILTGLNEAFIIDNAKRQEILDNCIDDAERERTA
                     AIIKPILRGRDIKRYYYEWAGLWVIGTFPALKLNIEDYPAIKKYFLDNFDIRQLEQSG
                     KKYPELGFNARKKTNNKWFEIQDTIAYYPEFEKEKIVYGQFQDDAE"
BASE COUNT          839 a          563 c          536 g         1118 t
ORIGIN      
        1 attctgcatc atcttgaaat tgtccataca caattttctc cttctcaaat tctggataat
       61 aggctatggt gtcctgaatc tcaaaccatt tattattagt ttttttacga gcattaaaac
      121 ctaattcagg atattttttc cctgattgtt ctaattgcct aatatcaaaa ttatcaagaa
      181 aatatttttt aattgcagga taatcttcaa tatttagttt caaagcaggg aatgttccga
      241 tcacccacag cccagcccac tcataataat accttttgat gtctctacca cgtaatatcg
      301 gctttatgat agctgccgta cgctctcgtt ctgcatcgtc tatgcaatta tccaaaattt
      361 cctgtcgttt agcattgtct attataaagg cttcgtttaa gccagttaat ataccacgat
      421 aaatttttac attccagtct ttcaatggtt taccaatacg ttcgattttt ttcttcaatt
      481 gctgctcggc ttcgttgcca atgtaccaag cttctttggt aagattttgc aaaataactg
      541 ccttttcctt tatttgttga gctatgtcaa tgggctgagc attggttttt tgaatggtaa
      601 ctgcttgcaa caaataagtt tttgcagtat ttttgctttt ctgcagcagc aaaatattgg
      661 tatccactgt ggcactttca aaaacacccg ctcctaaatc gaccagcaaa agcggctgat
      721 atttcaggaa aaatgttctt aatttttcgc cataaccggc tcgcatccat ttattggaac
      781 tgatataaca caacaaacct tcgtttttca gaattgttat gcctttttcg taaaacaaac
      841 aataaatgtc gcctgtgcgg tcaaaagttt cgtaatgctg attttggtaa aggtcggcaa
      901 attttctttt gtcgtcgtaa gctttttgca gttgaatgta aggtggattt cctataacca
      961 cgtcaaagcc gacaaagttg ccgtcgtcgt ccagcacttc gggaaactcg aaacgccatt
     1021 caaaagccct attataaata gtttttagtt tctcattata gatttcttcc aattcgtgca
     1081 cttctttgct caattcgtcc agcttgtttt tccattcttt ctgtgcattt tcctcaaagc
     1141 tgatgggttg ttgcatgaag ttcagatatt cattttgttt ttccttccat tttttgtaat
     1201 cggtatccga aggatcagct acactcataa actgcttttt cagtcgctga atttcctgtt
     1261 cagtttgatg tcgggttgcc ctgtcggtgg tacttttgta aataataacc tgctgtttat
     1321 atttttggat aatgtatcta attttttgct gtgcagcagg caacaagttg tagtgtttat
     1381 cggtaaggtt aaatcggcta atcaacgaat cgccacattt gatgttgata tcgatattgg
     1441 gcaatgtttc caattctttg aagtcgggag ctttgtaata cgcatttttc agcagttcaa
     1501 tccataatct cagccgacaa atcaataccg atttgggatt aatgtccaca ccaaaaagac
     1561 agttttcgat gagcaatcgt ttttcattga ataaagtcat ttgtacttcc tgagcatcag
     1621 gagcaggttt ttcgtcatcc atcagaaaat attcataagg ctgattgtta aatttatggg
     1681 taacagtcaa ttcatcgttg tccacgctca cttcccaatt aataatgatg ccgttgtaag
     1741 aaagaatgtt caattcactt ttcacagcaa ttaattcgtt gagtgccgag acaagaaaat
     1801 gtccagagcc gacagccggg tcacaaattt tcaaggaatt gatatgatcg ttaaatcgtt
     1861 caatatcttc ttttttgtga tgtaatcgac aataattttt tacatcgtcg aaatcattaa
     1921 attccttgtt ttctttttct ctgaattttt gaaccacagc tttgcgaatg gtttcacggc
     1981 tcataaacat ggtgatgaag ccgggcgtaa aaaaggcgcc atctttgtaa ccatttatct
     2041 tttcaaaaat caatccaagc acagaagcgt tgataatggt tcggttatct tcctgaattt
     2101 tagcatggct gtcgctcgaa aagttatagg aagaaaggaa ttgcagtaaa tattgcaaag
     2161 tgtttatttc tttcttgttg gtagcaattt ttagaactga tttgttataa atcggcaatt
     2221 gctggtcgtt tttcagattg gcaatggaaa tagtttgctg ttcgagtaag ctcatttcaa
     2281 aaagcgagct attcagatag ggaatatgtg cgtattgttc ttgaattttc tgagtacgtt
     2341 cagcaggctt tatggcaaaa acttcaaaaa acaattcatc aagtttgtca aattccttta
     2401 ctttatcggg cgatagaaaa gcaaaatttg aatcgttgtt gtgatatttt ataagttgtc
     2461 cttccagcag ttttaaaaac agaattctgt tcagccaggt gatgcacaat tcgagtgcta
     2521 cgctgaaaag ctgttcttct tctgtttgtc caaattgggt aatatcttca attcctttta
     2581 atttgtctct ggtttttaga atgttaatgg tattttctat caagctggct tcgcttctgt
     2641 ctttgcttcg gcgttcaatg actattttgt tgttttccga tttttcttcg agccccagaa
     2701 tgtaaagcaa ttcattataa aattccttat tcagtgtatt gctgtcgttg gcaaatggtt
     2761 ttttaagcag atgttcaggc gaaaatattt tatacaagtt gatgagttga cttaaatttt
     2821 cactgtcatg gctgtttaaa gcagcttcat aatcacgcaa atcaaaatat gtggctttta
     2881 actcggcaac atttttatca atgaaatttc gaatattttc atagagtgaa acagttgtat
     2941 tgtccacttt ttgtttggta tcccaatttt cgtaattgtt tctgaattcc tgatttttgt
     3001 agaaaatatt ttcgaaatct accgcatcaa atagatacca ttcatagcaa ttggta
//
LOCUS       DAOTKS010000194         3036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_26578_length_3036_cov_4.581013, whole genome shotgun sequence.
ACCESSION   DAOTKS010000194 DAOTKS010000000
VERSION     DAOTKS010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26578_length_3036_cov_4.581013"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="P5236_04590"
     CDS             complement(<1..194)
                     /locus_tag="P5236_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated lipoprotein GldK"
                     /protein_id="HRU63673.1"
                     /translation="MKKFLSFIIVSCIGSLLFIGCNKPAGELVGIGTKGTFHEANPYG
                     MVLIKKGTFLMGANDQSAIGA"
     gene            complement(238..1185)
                     /locus_tag="P5236_04595"
     CDS             complement(238..1185)
                     /locus_tag="P5236_04595"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRU63674.1"
                     /translation="MKKIVLLILFSFVSIGVIVPQTDSQLSQYMFNPSIYNPAAAGEN
                     GMMEISWQHRIQWVGIPNAGQSSFYNVNSPVNLFSNQHGIGLQVVNENIGLFSNLDFH
                     FQYAFKKDLAGGILSIGASLGFLDIGFKGDSVHFPTIGEYYESNDPLIPTTSVSGIGF
                     DVGLGVWYSNTKGYAGFSYAHLNQPVINWGENLAYKQVGTLYLTGGYNWTLNDPKYII
                     KPSLLFKSDLNSYQVDISAFLEYNSKYWGGLSYRFQDAVVLFGGINAIAGLIIGYSYD
                     LPVSQIIRGSSGSHEFLIAYNFDIKLNKAKHKHKSIRIL"
     gene            complement(1352..1573)
                     /locus_tag="P5236_04600"
     CDS             complement(1352..1573)
                     /locus_tag="P5236_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HRU63675.1"
                     /translation="MYWTLELASKIEDAPWPATKDELIDYAIRSGAPLEVIENLQEIE
                     DEGEIYESIEDIWPDYPSKEDFFFNEDEY"
     gene            complement(1637..2188)
                     /locus_tag="P5236_04605"
     CDS             complement(1637..2188)
                     /locus_tag="P5236_04605"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRU63676.1"
                     /translation="MKLYTKKGDKGQTSLRGNTMILKNHVRLEAYGTIDELNSFIGLL
                     MTFSLDESNLNYLKRLQNNLFTIGSNLATDTSKIPLTKTSILESEEITFIEQEIDRLT
                     EAVPPIKEFILPGGTTVAAISHICRTVARRAERRLYDLNQEEMVDKRILIYINRLSDY
                     FFALSRFINHQNGIKDIFCEKKE"
     gene            complement(2320..>3036)
                     /locus_tag="P5236_04610"
     CDS             complement(2320..>3036)
                     /locus_tag="P5236_04610"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRU63677.1"
                     /translation="GFGVHSPFIYHFISFIIEEKNPYYIFKKIESLRSKLLKDKRKIK
                     INDFGTGNNRISTVREIARHQLKSAKYGQLFFRIAENNKVQNVLELGTSLGVTTAYLA
                     SASSQINCITLEGCSEVAEIARENFSCLDISNIKIVVGNIDQTLKNALDEFESLDLVF
                     IDANHRSTALLNYFEQCLTKIHTNSILLIDDIYWSTDMEKAWKKIKENPHVTATIDLF
                     HMGIVFFNPHLYKKHYKVRF"
BASE COUNT         1045 a          508 c          416 g         1067 t
ORIGIN      
        1 gctccaattg cagattgatc attagctccc attaaaaaag ttcctttttt gataagaacc
       61 attccataag gatttgcttc atgaaaagta cctttggttc ctattccaac caattcacct
      121 gcaggcttat tgcacccgat aaataataaa gagcctatgc aactaacgat aataaaggaa
      181 agaaattttt tcattcttgt atgtttttaa ataaaaaact tgtagatcaa attaaagtta
      241 taaaatacga atacttttgt gtttatgttt tgctttattt aatttaatat caaaattata
      301 ggctattaaa aattcatgcg aacctgaact tcctcgaatt atttgcgata caggaagatc
      361 gtaagaatag ccaataatta aaccagcaat agcattaatc cctccaaaca atacgacagc
      421 atcttgaaaa cgataagaaa gacctcccca atatttgcta ttatattcta aaaaggctga
      481 tatatctacc tgatatgagt taagatcact tttaaacaac aagcttggtt tgataatata
      541 ttttggatca ttcagtgtcc aattgtaacc acctgttaaa taaagagttc caacttgttt
      601 atatgctaaa ttttctcccc aattaattac tggttgattt aaatgagcat aagaaaaacc
      661 agcataacct tttgtatttg aataccaaac acccaaacca acatcaaaac caattcctga
      721 gacacttgtt gtaggaatta aaggatcatt tgactcataa tattcaccta ttgtagggaa
      781 atgaacacta tctcctttaa atccaatgtc cagaaaacct aacgaagctc caatacttaa
      841 aattcctcct gccaaatcct ttttaaaagc atattgaaaa tgaaaatcca gattagaaaa
      901 cagtcctatg ttttcattaa ctacctgaag ccctatacca tgttgatttg aaaacaaatt
      961 tacaggagaa ttaacgttat aaaagcttga ttgccctgca tttggaattc ctacccactg
     1021 aatacgatgt tgccacgaaa tctccatcat tccattttct cctgccgctg caggattata
     1081 aattgatggg ttgaacatat attgacttaa ctgggaatcg gtttggggaa caataacacc
     1141 tattgaaaca aaggaaaaca aaattagcag cacaattttt ttcataggtt acatttcctt
     1201 tttgtccctt cccatatgta agaaaaatga ctattctctt aaaattaact ttaaattaac
     1261 aaaaaaattg tatcctctta ttttttgttt taaagtttta tttcagcaaa aataagtata
     1321 gtttttttaa aaataaaatt aagattaaaa attaatattc atcttcattg aagaaaaaat
     1381 cttctttgct gggataatca ggccatatat cttcaattga ttcataaatt tctccttcat
     1441 cttctatttc ctgcaaattt tcaattactt ctaaaggagc tcctgaacga atggcatagt
     1501 caattaattc atctttggta gctggccatg gggcatcttc aattttagaa gctaattcta
     1561 atgtccaata catagattaa gtttttaaaa ttgttatttc ataataacgc gcaaaaatat
     1621 ataaaaaatt ttcagtttac tccttttttt cacaaaaaat atcttttatc ccattctggt
     1681 gattaataaa acgcgataaa gcaaaaaaat aatcagacaa gcgattgata tatattagaa
     1741 tgcgtttatc aaccatttcc tcctgattta aatcataaag tcttctttcg gctcgtcgag
     1801 cgacagtgcg gcaaatatgg cttatagcag caactgtggt accgccaggt aaaataaatt
     1861 cttttatagg aggtactgct tcggtgaggc gatcaatttc ttgttcaata aaagttattt
     1921 cttcactttc taatatagaa gttttggtca aaggtatttt tgaggtatcg gttgctaaat
     1981 ttgaaccaat agtaaataaa ttattttgaa gtcgttttaa ataattcagg tttgattcgt
     2041 ctagtgaaaa tgtcattaat agaccaataa atgaatttaa ttcatcaata gttccatacg
     2101 cttcgaggcg aacatgattt ttcagtatca ttgtgttgcc acgcaaactt gtttgtccct
     2161 tgtcaccttt tttagtatat aatttcatct tgactgttct tgttgatttg aaggaagttt
     2221 tagaaagttt tcatttttaa tgttttctct tagtacgtaa caatgaattt gattttttgt
     2281 ttattaactt ttgaaaaact cttattgtaa gttatgaatt tagaaacgta ctttataatg
     2341 ttttttgtat aagtgaggat taaaaaaaac aatccccata tgaaaaagat caattgtcgc
     2401 cgttacatgt ggattctctt ttattttttt ccatgctttt tccatgtcgg ttgaccagta
     2461 aatatcatca atcaataaaa tactattagt atgtatcttt gttaaacatt gttcgaaata
     2521 attcaataag gctgtagagc gatgattggc atcaataaat actaaatcca gactttcgaa
     2581 ttcatccaat gcatttttaa gcgtttgatc aatatttcca acaacaattt tgatattcga
     2641 tatatcaaga caagaaaaat tctctctggc aatttctgca acttcagaac atccttctaa
     2701 tgtaatacaa ttgatttgag aagaagcact tgccaaataa gctgtggtaa cacccaatga
     2761 agtgcccaat tccaaaacat tctgaacttt attgttttca gcaattctga aaaataattg
     2821 tccatatttt gccgatttta attgatgacg tgcaatttcc cgaacagtgc tgatcctgtt
     2881 atttcctgta ccaaaatcat ttattttaat tttcctttta tcttttaata attttgaacg
     2941 caaagattca attttcttga agatataata gggatttttt tcttctataa taaaagaaat
     3001 aaaatgatag ataaaaggag aatgaacacc aaaacc
//
LOCUS       DAOTKS010000195         3028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_26670_length_3028_cov_3.176589, whole genome shotgun sequence.
ACCESSION   DAOTKS010000195 DAOTKS010000000
VERSION     DAOTKS010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26670_length_3028_cov_3.176589"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /locus_tag="P5236_04615"
     CDS             <1..1104
                     /locus_tag="P5236_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63678.1"
                     /translation="NAKLSVKVEVMNSEGGVDSLTVSNVTVKRPTFNNLYLQLENGEV
                     LEMTKRITDTNVFECSWVLSNDINVKVISNATTPQNGLIWGNVKGTFDLSQDGTVGFF
                     NLVDPLIVPSKITFNILTFDLEFEGTTMDDGNMWIGMAKLTQETGIDPNRFPGITVMK
                     GTGHFTKNKQVYFRGFPNSVNISKILNPDFFEMRDGKYYFTQETDDYIIRYDMSIGFI
                     FIYPVDSNYPKGIYIAGWGFGPPVQPYTTVRSDYNFDDETLPPGVFYFMPKIADGVYA
                     ATVYLAVDSDDFIGFNFYYQKGWGDPVGYGPGGKAEVTGAELAYSTNDAFPLSNGAIW
                     RENIHDPKFTNGVYLLKIDFNKRELSATLKTAN"
     gene            1227..2903
                     /locus_tag="P5236_04620"
     CDS             1227..2903
                     /locus_tag="P5236_04620"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HRU63679.1"
                     /translation="MKIKNIIILLILFVNVGCGKSTIPDHNMPVEPSTLFSGLQTIDT
                     VFPLIPQPVKISAYNSFCNIKSIKVIYISNENLRPAAYYLSEILSEIHSDKLDILVGN
                     SRTKNALNLITEPLSENTEQYKLEIAPDNLTVIGTTPQAVICGINTFRQFIALNSILS
                     PAFNTRFPCVAIEDEPVFHWRAFMLDVARHFFTKEEICQTMDMMALLKLNKLHLHLTD
                     DEGWRIPIDKYPEFLETGWKRFPNDQDSFCLEMAKSYPDYKFPSDRWDGTSYFGKFSK
                     TEIKEMIEYGLERGIEIIPEIDIPGHSTVAIKAHNNFACVGKGVGWGKEFSYPLCLGN
                     DSVLNYYTDIFNEIMELFPSKYIHVGADEADPTNWISCPKCQARITNYNLISIPGLER
                     WFLNKIEKNIREKGRKMIVWDDALSMVGSSASIMWWRDWIGINNAVGGAVRQGSEAII
                     ANWDILYFSGNEKNTSLSDIYHLPLYPAGISEGQKNLIIGIQACVFTESIPNIGRLNF
                     MIYPRLFALSEKAWYADKTSDEMWNTFKTRLNLWLLYLRSKNIRYRTPNF"
BASE COUNT          972 a          513 c          565 g          978 t
ORIGIN      
        1 aatgcaaaat taagtgtaaa agtagaagtt atgaattcgg aaggtggagt cgattcctta
       61 actgtgagca atgttacggt gaaaagacct actttcaaca atctttacct gcaattggaa
      121 aatggtgagg tgctggaaat gacaaaacgt attaccgata ccaatgtgtt tgaatgttca
      181 tgggttcttt caaatgacat aaatgtgaaa gtaattagta atgccacaac tccacaaaat
      241 ggacttatct ggggaaatgt taagggaaca tttgatttgt cgcaagacgg aacagttggt
      301 ttttttaatt tggtcgaccc gttgattgtg ccttcaaaaa tcaccttcaa tatcttaact
      361 ttcgatttgg aatttgaagg aacaacaatg gatgatggta atatgtggat tggcatggcc
      421 aagttgactc aagaaacagg aatagatcca aatagattcc caggaattac cgtaatgaaa
      481 ggaaccggcc actttactaa aaataaacaa gtttattttc gcggtttccc taattcagtg
      541 aatataagca aaatactgaa tcctgatttc ttcgaaatgc gtgatggcaa atattacttc
      601 acccaggaaa ccgatgatta cattattcgt tatgatatga gtatcggatt tatatttatt
      661 tatcctgttg acagcaatta tccgaaaggt atttatattg caggctgggg atttggccct
      721 cctgttcaac cttataccac tgttcgttct gattacaact ttgatgacga aaccttgcct
      781 ccgggagtct tttattttat gcctaaaatt gcagatggag tttatgccgc tactgtttat
      841 ttggcagttg actccgatga ttttattgga ttcaatttct attatcagaa aggttggggc
      901 gatcctgttg gttatggacc gggaggtaaa gccgaagtaa ccggagcaga actcgcatat
      961 tctacgaacg atgcatttcc tttaagcaac ggggcaatat ggcgcgaaaa tattcacgat
     1021 cctaaattta ccaatggcgt ttatcttttg aaaattgatt tcaataaacg tgaacttagc
     1081 gctacattaa aaactgctaa ttaattttat ccaatgaact gttcaggaca gagtttttcc
     1141 cctttgtcct gaacttttaa ttttatattg ttaaaattta atgatattgg caagaaaaat
     1201 actctccata aaacaattat caccacatga aaattaaaaa tatcattatc ttactcattt
     1261 tatttgttaa cgttggttgc ggaaaatcaa ccattcctga tcataatatg cctgttgagc
     1321 cctcgacatt attttcggga cttcaaacaa tagatacagt ttttcctctt attcctcaac
     1381 ctgtaaaaat atctgcctat aacagttttt gtaatattaa atctataaaa gtcatttaca
     1441 tttccaatga aaaccttcgt cccgctgcct attatctttc agaaattcta tcggaaattc
     1501 attctgacaa attggacatt ttggttggga acagcaggac aaaaaatgct ttgaatttga
     1561 taacagagcc tctatcggaa aatacggaac aatacaaact cgaaatagct ccagacaatt
     1621 taacggtcat tgggacaact cctcaagctg tgatctgtgg tattaatact ttcagacaat
     1681 tcattgcctt aaatagcatt ctctctcccg catttaatac tcgtttccct tgtgtagcca
     1741 ttgaagatga acctgttttc cactggagag cctttatgtt ggatgtggct cgtcattttt
     1801 ttacaaaaga agaaatctgt caaacaatgg acatgatggc tttgttgaaa ttgaataaac
     1861 tacacttgca tttaaccgac gatgaaggat ggagaatccc tatagataaa tatccggaat
     1921 ttcttgaaac aggctggaag agatttccaa atgaccagga cagtttctgc ctcgaaatgg
     1981 cgaaaagtta tcctgattac aaattccctt ccgatcgttg ggatggtaca tcatatttcg
     2041 gaaaattctc taaaaccgaa ataaaagaaa tgattgaata cgggctggaa agaggtatcg
     2101 aaattattcc tgaaatcgac attccgggac attctactgt tgccataaaa gcgcataata
     2161 attttgcttg tgtaggaaaa ggagtcggtt gggggaaaga attttcttat ccgctttgtt
     2221 tgggaaatga ttcggtatta aattattata cggatatttt caatgaaata atggaacttt
     2281 ttccttctaa atatattcat gtcggtgcag acgaagcaga tccaaccaat tggatttcat
     2341 gtccaaaatg tcaggcaaga atcacaaact acaatctgat ttctattcct ggactggaac
     2401 gatggttctt aaataaaata gagaaaaata ttagagaaaa agggcgtaaa atgatagttt
     2461 gggatgatgc gttgagcatg gtaggttctt cagcttctat tatgtggtgg cgagattgga
     2521 taggaataaa caatgctgtt ggtggagctg ttcgacaggg ctcagaagcc attatagcaa
     2581 attgggatat tttgtatttt tctggaaatg aaaaaaatac atctctttca gatatttatc
     2641 atctccctct ttatcccgca ggtatttccg aaggtcaaaa aaacttgatt atcggtattc
     2701 aggcttgtgt ttttactgaa agcattccaa acattggtcg attaaatttt atgatttatc
     2761 ccagattgtt tgctctcagt gaaaaagcat ggtacgccga taaaacgtct gacgaaatgt
     2821 ggaatacatt caaaacaaga ttaaatttat ggctgcttta tcttcggtca aaaaatattc
     2881 gttaccgaac acccaatttt tagtacattc ttcaatttct cttccacaaa atgaggttta
     2941 taaaaaactt tatgagaaaa ttgaattttt ataattaatt tagatacaat ttttgctcat
     3001 ttcaagaata gtatttagtt ttgtaaaa
//
LOCUS       DAOTKS010000196         3026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_26696_length_3026_cov_3.178728, whole genome shotgun sequence.
ACCESSION   DAOTKS010000196 DAOTKS010000000
VERSION     DAOTKS010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26696_length_3026_cov_3.178728"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            452..1465
                     /gene="dusB"
                     /locus_tag="P5236_04625"
     CDS             452..1465
                     /gene="dusB"
                     /locus_tag="P5236_04625"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HRU63680.1"
                     /translation="MKIGNIDFPNYPLFLAPMEEVTDLPFRLLCKQFGADLVYTEFVS
                     SDALIRKIERSLQKITFFESERPIAIQLYGQHVDAMVQAALMAEELQPDIIDLNFGCP
                     VKKVANKGAGAGLLKDIPKLLEITKAVVKAVKLPVTVKTRLGWDENNKIILTLAEQLQ
                     DCGIQALTIHARTRSQMYRGEADWTLIGEVKNNPRIKIPIIGNGDVTTPQKAKEYFDK
                     YGVDAIMIGRGSLGQPWIFKEMKEFLEKGEISSSLSFQQKKEILKNYILSCIQWIDFK
                     NEEVNFETISNIRLKGIIHARCHLAATPIFKNIPNFKNTRIAMLRAETIQELFQIIDN
                     VNI"
     gene            complement(2240..>3026)
                     /locus_tag="P5236_04630"
     CDS             complement(2240..>3026)
                     /locus_tag="P5236_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HRU63681.1"
                     /translation="TFVDKQDFLDAVDRIIGGLERRNKITTKSEKRSIAYHEAGHATI
                     SWMLEHANPLVKVSIVPRGEALGAAWYLPEERQLTVREHMLDEMCATLGGRAAEEVFL
                     NQVSTGAINDLEQVTKRAYAMVTYFGMSEKLPNLCYYDSTGSSEFSFTKPYSEKTAEL
                     IDAEVKKIVNEQYLRAKEILKKYAEGHNKVAELLIEKEVIFAEDMEKIFGKRIWASRS
                     DELLAQNSENQSSQKNEESKSDENLEENTQETSTKASAFQQIK"
BASE COUNT          979 a          506 c          482 g         1059 t
ORIGIN      
        1 acatttgagg aagtaaagta acttctaaaa caaccaataa aaaactaaat ctctgtaaac
       61 gaatggtttg cagagatttt ttgtacttgt ttttacctat tttctgcatt ataacaccta
      121 aaaattttta taaacaattc ggtaaaagtt ctgtttgttc ctcgcaggtc aatttgccag
      181 cccttttcga aaagataatc gacttctctt catacaagat tctttccccc gattcttgaa
      241 tatgcaataa gccgtaaaaa caatttgtcc aacttcaagt tcaaattttc gcttaatttt
      301 atacaaaaaa cgataaattg agtctatctc aatatctttt tcttgataag aagttttttt
      361 tattttccaa taaacctgta aatttgtaat tgaaacaatt gtttttaatt atttgtacag
      421 ataaaaatta aaattcttta cctgttattg aatgaaaata ggcaacattg atttcccaaa
      481 ctatcctttg tttttagctc caatggaaga agtaaccgat cttcctttcc gattgttgtg
      541 taaacaattt ggtgctgatt tagtttatac agaatttgtt tcttcagacg ctttgatcag
      601 aaaaattgag cgatcacttc aaaaaattac ttttttcgag tccgaacgac cgatagctat
      661 tcaactctac ggccaacatg tggacgcaat ggtacaggca gctttgatgg ctgaagagct
      721 acaaccagat attattgact tgaattttgg ctgtccagtg aaaaaagttg caaataaagg
      781 tgctggtgct ggattgttga aagatatacc aaaattattg gaaattacta aggcagtagt
      841 gaaagcggta aaattacctg taactgtaaa aactcgtctt ggctgggacg aaaacaacaa
      901 aattattctt actttagcag agcaattgca ggattgcggt attcaagcat taaccattca
      961 tgcacgtact cgttcgcaaa tgtacagagg cgaagcggat tggactttga ttggggaagt
     1021 aaaaaataat ccccgtataa aaattcctat tattggaaac ggagatgtaa ctactccgca
     1081 aaaagcaaaa gaatattttg ataaatacgg agtggatgct atcatgatag gcagaggaag
     1141 tttaggacaa ccatggattt ttaaggaaat gaaagaattt ctggaaaaag gtgaaatttc
     1201 ttcgtcttta tcatttcaac agaaaaagga aattcttaaa aattatattc tttcctgtat
     1261 tcaatggatt gattttaaga atgaagaagt aaattttgaa acaatatcaa atattcgttt
     1321 aaaaggaatt attcatgctc gctgccattt ggcagctaca cctattttta aaaatattcc
     1381 aaattttaaa aatacacgca ttgccatgct tcgagctgaa actattcagg aactttttca
     1441 aatcatagac aatgtaaaca tctaattcat ttttacttga tttttaattc tgtcattttt
     1501 tatcaatttt aatcaaaaga attccttgag gcttgcctcg gggttattca ttgtagaaat
     1561 tatgaccatt gatggtcaaa ttatttggtt aaagcaaaat gtaaaatctg gagatgtcgt
     1621 ttttcctcaa aatattcctc aaggtatatt tttccttttt ataaaaaaat ccaatgaaat
     1681 aatttctatt ttaaagctca taaaaaaata agtcaagcca taacttcgca aaatttggtg
     1741 tatatgtcaa aaaaataggc ttttttaaga acgtttttct ctgggttcca tgtattagtt
     1801 acctcataga gataataata tccatttaga aaacaaagtt tagtccattt cttacggtat
     1861 tttaataccc attctggagc ctgacataac gcgataattt ttaaatttta atggttggtt
     1921 taattacgta taaattacga aattagaaat taattctttg ggattgcgaa cgcccctaac
     1981 ggcatttttg caatatcaaa tgttttattt ggtcctttaa tagtagaaat gaaggaaaaa
     2041 aagatgacga ttaatttaac ttaaattgct cgtaaatctt agcttgttaa atatttatta
     2101 gaaatttacg agcactcacc aatatgcaat gcagatacct ttatcaaaat aaaatgtaat
     2161 gaaacgagat ttcatttttt aatggtctga tttgcttcat ttcctctatt tttcaaaagt
     2221 tttgcctgaa gaaaatatac tatttgattt gttggaatgc agaagctttt gtagaagttt
     2281 cttgtgtgtt ttcttcaaga ttttcatcag atttactctc ttcgtttttc tgagaagatt
     2341 gattttcaga attttgagca agaagttcat cgcttcttga agcccaaatt cgtttaccaa
     2401 agattttttc catatcctct gcaaaaataa cttccttttc aatcagtaat tctgccactt
     2461 tattatgccc ttcagcatac ttttttaata tttcttttgc acgaagatat tgttcattga
     2521 caattttttt aacctcagca tcaatcaatt cggctgtttt ttcgctataa ggctttgtaa
     2581 atgaaaattc agagcttccc gtggaatcat aatagcataa attaggtaat ttttcactca
     2641 ttccgaaata agtaaccata gcataggcac gtttggtaac ttgttcgagg tcattaattg
     2701 ctcctgttga aacttgattc aaaaaaactt cttcggctgc tctaccgcct aaggttgcac
     2761 acatttcgtc cagcatgtgt tcgcggacgg tcaattgacg ttcttcaggg agataccacg
     2821 cagctcccaa tgcttcgcca cgaggcacaa tacttacctt aaccaacgga tttgcatgtt
     2881 ccaacatcca actgatagtg gcatggcctg cttcgtggta agctattgat cttttttcag
     2941 attttgtagt gattttattc cgtctttcca atccaccaat gattcggtct actgcatcaa
     3001 ggaaatcttg tttgtcaaca aaagtt
//
LOCUS       DAOTKS010000197         3021 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_26733_length_3021_cov_5.065071, whole genome shotgun sequence.
ACCESSION   DAOTKS010000197 DAOTKS010000000
VERSION     DAOTKS010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26733_length_3021_cov_5.065071"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..628
                     /gene="ilvN"
                     /locus_tag="P5236_04635"
     CDS             74..628
                     /gene="ilvN"
                     /locus_tag="P5236_04635"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRU63682.1"
                     /translation="MENTTLYTIIIFSENKVGVLNQITVIFTRRGLNIETLSVSPSAI
                     PGIHKFTITVFTTEEVVKKVVQQIDKRVDILKAYYNTDDDLVHQEIALYKVATDKLLA
                     RGSIEGLIRKYQIHILEITENCVVFEKTGHYEDTQKLFEELSQTIGVLQFIRSGRIAI
                     TKSKVERLSDMLEELKKLEEKIDK"
     gene            809..1858
                     /gene="ilvC"
                     /locus_tag="P5236_04640"
     CDS             809..1858
                     /gene="ilvC"
                     /locus_tag="P5236_04640"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRU63683.1"
                     /translation="MAKINFGGVIEDVVTREEFPLSKALEVLKDEIIAVIGYGVQGPA
                     QSMNLRDNGFNVIVGQAPEFKDDWDKAVNDGWVPGKTLFSVEEAASKGTIIQYLVSDA
                     AQRILWPTIKPHLTKGKALYFSHGFSITYKDYTGVVPPADIDVIMVAPKGSGTSVRRN
                     FLAGSGINSSFAVFQDATGKALERTLALGIGIGSGYLFPTTFEKEVFSDLTGERGVLM
                     GALAGIMDAQYEVLRKNGHSPSEAFNETVEELTQSLIRLVDEKGMDWMYANCSATAQR
                     GALDWRPKFKEAVMPIFEDLYQRVKDGRECKRVLESTGVKNYKELLEKELKEMRESEI
                     WAAGAEVRKLRPKTN"
     gene            complement(2006..2821)
                     /gene="ispE"
                     /locus_tag="P5236_04645"
     CDS             complement(2006..2821)
                     /gene="ispE"
                     /locus_tag="P5236_04645"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRU63684.1"
                     /translation="MIVYPNAKINIGLNVIEYLPDSYHSIETVFYPIGLYDTLEIKPI
                     ENDKPVFSTSGLAIEGNPENNLVFKAYQLLHNSYHIPPVKIMLDKKIPSAAGLGGGSA
                     DAAFTLKALNQLFNLNLSTQELESLAVKLGADCAFFIHNQPGFAIGKGNIFKPISLNL
                     KNFYLLLVKPPISVSTAEAYSLVKVRKPEISLEENIYKPITEWKNLIKNDFEDPLFER
                     YPILANIKENLYKMGALYASMSGSGSTIYGIFDEKPEKNKNFRNFFLSLIQLA"
BASE COUNT         1058 a          415 c          529 g         1019 t
ORIGIN      
        1 ttaaaaatta aaactgacaa attctgattt ttgtcggtta atagtgaaaa aatttattaa
       61 tcaaattctc aacatggaaa atacaacctt atatacgatt attatttttt cagaaaataa
      121 ggtaggcgtt ttaaatcaga ttacagtaat ttttacacga cgaggcttaa atatcgagac
      181 tctatcagta tctccttctg ccataccagg aatccataaa tttactatta ctgtttttac
      241 tacggaagaa gtggttaaaa aagttgtcca acaaattgat aaaagagttg atattttaaa
      301 agcttattat aataccgatg atgatttggt tcatcaagaa attgctttgt acaaagtagc
      361 aacagataaa ttgctggcaa ggggttccat tgaaggtttg atacgtaaat atcaaattca
      421 tattctcgaa ataactgaaa attgtgtggt ttttgaaaaa acgggacatt atgaagatac
      481 tcaaaaactt ttcgaagaat taagtcagac aattggagtg ttgcaattta ttcgttcggg
      541 acgtatagct atcactaaat caaaagtaga acgattaagt gatatgctcg aagaattaaa
      601 aaagttagaa gaaaaaatag ataagtaatc ggttttttaa tcaatagaat tcctcgagac
      661 ttgcctcggg gttatttatt aaaaaataaa ttttttatca gaaaaaatta tttgtgtaat
      721 aacaattaag atactaattt taactttaaa ataaaagttt tagaataaac aattttaata
      781 ttttttaatt tttaaaaata aaataaagat ggcaaaaatt aattttggcg gcgttatcga
      841 agatgtggta acccgcgaag agtttccatt aagcaaggct ttggaagtat taaaagatga
      901 aattattgca gtaataggtt atggtgtaca gggtcctgca caatcaatga atttacgtga
      961 taatggcttc aatgttattg ttggtcaggc tccagaattc aaagatgact gggataaagc
     1021 tgttaacgac ggctgggttc caggcaaaac tttattttca gtggaagaag ctgcatccaa
     1081 aggcactatt attcaatatt tagtttctga tgctgctcaa agaattttat ggccaactat
     1141 taagccgcat cttaccaaag gaaaagcttt atatttttca catggttttt caattactta
     1201 taaagattat actggagttg tacctcctgc tgatattgat gtaattatgg ttgctcctaa
     1261 aggttctgga acgagcgtgc gtagaaattt cttagctggt tcaggcatca atagtagttt
     1321 tgctgtattt caagatgcaa caggaaaagc tttggaacgt actttagctc ttggtattgg
     1381 tataggctca ggatatcttt tcccaactac ttttgaaaaa gaagtattta gtgatcttac
     1441 tggtgaaaga ggcgttttga tgggtgctct tgccggaatc atggacgctc aatatgaagt
     1501 gctcagaaaa aatggtcata gcccttcaga agctttcaac gaaacagttg aggagcttac
     1561 ccaaagcctt atccgtttgg ttgacgaaaa aggtatggat tggatgtatg ctaattgctc
     1621 agctactgca caacgtggtg cacttgattg gcgtccaaaa tttaaagaag ctgtaatgcc
     1681 tatttttgaa gatctttatc aaagagtaaa agatggtaga gaatgcaaac gtgtattgga
     1741 atctactggt gttaagaatt ataaagaatt gcttgagaag gaattgaaag aaatgcgcga
     1801 aagtgaaatt tgggctgcag gtgctgaagt tagaaaatta cgtccaaaaa ctaactaatt
     1861 atttttatca ttttttcata ttttaaaagt cttgtttcat cacatttgtt gagacaagac
     1921 tttttattct ttcccagcta aaacttgatt ccaaaaagtt atattgttga ttcactatac
     1981 attatatgca attaaatttt atgcattatg ctaattggat aagggaaagg aaaaagtttc
     2041 taaaattttt atttttttcg ggtttttcat caaaaattcc ataaatggtc gatcctgaac
     2101 ctgacatgga agcataaaga gctcccattt tgtatagatt ttctttgata tttgctaaaa
     2161 tgggataacg ttcaaaaaga ggatcttcaa aatcattttt tattaaattt ttccactctg
     2221 tgattggttt ataaatattt tcttccagag aaatttctgg ttttcgcact tttaccagtg
     2281 aataagcttc agcagttgaa acagatatgg gaggttttac taaaagcaga taaaaatttt
     2341 ttaaatttag agatattggc ttaaaaatgt ttcctttccc aatagcaaag ccaggctgat
     2401 tatgaataaa aaaggcacaa tctgctccca attttacagc taacgactca agttcctgag
     2461 tgctgaggtt taaattaaat aattgattaa gagcttttaa tgtaaaagct gcatcggctg
     2521 agcctccacc aagtccagca gcagaaggaa tttttttgtc caacataatt ttaactggag
     2581 gaatatgata actattatgt aacaattggt aagctttaaa aaccaaattg ttctcaggat
     2641 ttccttcaat tgctaaacca gaagttgaaa aaacaggttt atcattttct attggtttaa
     2701 tttccaatgt atcataaagc cctatcggat aaaaaactgt ttcaatagag tgataactat
     2761 ctggcaaata ttctattaca tttaatccta tattgatttt agcattggga taaacaatca
     2821 ttgatttaat tttaaaacaa aaattaccaa aagacaaatt tatcaataat ttaaatgcag
     2881 ttattatttt aaagttaaaa aattttttaa tcttttctat aacgaatgtt gtttatcttg
     2941 tgataatttt aaaggaaaaa attaatgagt agagtcaacc agcaagtgcg aaattataaa
     3001 aaatgtttga aaaactcatt t
//
LOCUS       DAOTKS010000198         3015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_26771_length_3015_cov_4.888851, whole genome shotgun sequence.
ACCESSION   DAOTKS010000198 DAOTKS010000000
VERSION     DAOTKS010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26771_length_3015_cov_4.888851"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..796
                     /locus_tag="P5236_04650"
     CDS             98..796
                     /locus_tag="P5236_04650"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HRU63685.1"
                     /translation="MNKKILLTLLLTFVIFISCKPVEKKQNKKIDSTLQPMELTTFDT
                     IYLLQPDTTGGKPLMYALQHRKSDREFEQKNLSLKHLSEILWAAYGVNRPNGKRTVPS
                     AMALYPLQVYAVLSNGIYFYEPNQHALIPTVKGDYRSLAGLQDFVYTAPLNLIFIADY
                     HKYNTDRKIPDENRIRLVSLDAAHSTQNVYLYCASEGLKCVVRAGAKEKELLEVLKLE
                     PNYYQFIVAQTVGY"
     gene            complement(887..>1888)
                     /gene="dgt"
                     /locus_tag="P5236_04655"
     CDS             complement(887..>1888)
                     /gene="dgt"
                     /locus_tag="P5236_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HRU63686.1"
                     /translation="HDMGNPPFGHSGEKAIATFFSEGEGQKLAEKVSPVEWKDLVCFE
                     GNANTFRLLSHQFAGRRKGGFVLTYNTLASIVKYPYASAYTNSQHSKFGFFNSEADDF
                     KKIADELGIINVESNSFHYVRYPLVFLIEAADDICYQLMDIEDAHKLKILSTEETKFL
                     FLNYFDENQKRQKMEVLNLVNDENEQIAYLRSSVIGKLVEECSQIFVDNEENILNGTF
                     HTSLIEQLPPLSARAYQYCSDVANSKIYCSKQVLEVELSGYKIILTLLENLIHAVLQP
                     QKAYSQQLLMRIPSQYEINHPTMYGKIMAVLDYISGMTDVYALDFYKKINGIDLPTL"
     assembly_gap    1889..1898
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1899..2218)
                     /locus_tag="P5236_04660"
     CDS             complement(<1899..2218)
                     /locus_tag="P5236_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HRU63687.1"
                     /translation="MNWEQLLSTKRFGLESYHEPRKDSRTEFQRDYDRLIFSPPFRRL
                     QNKTQVFPLPGSIFVHNRLTHSLEVSSVGRSLGINVARCLADCHPNCPIIHEIDAIVA
                     AACLA"
     gene            complement(2225..2947)
                     /locus_tag="P5236_04665"
     CDS             complement(2225..2947)
                     /locus_tag="P5236_04665"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HRU63688.1"
                     /translation="MIDKELGEIFFVHSNRAKYINIKILTDGLRVTLPVNSTKNDALK
                     FIDSLRPKIIKKQLLIKQRNEKNVIVLNKNTVFSTLTFQITIKEEKRENIYFELRNGW
                     LNIFVPENFNFQQPVMQKHLWKGVFYFLRQEAKHFLPKRTQKLAEQHNFKYKQVKIQS
                     SKTRWGSCSNHLNINLSFYLMLLPIHLVDYVILHELCHTVEMNHGTNFYQILERVTNG
                     NSNKLKSELKKYSSMKFPNNIE"
BASE COUNT         1046 a          516 c          438 g         1005 t
ORIGIN      
        1 ttgcattaat cacttgaatt tatgatataa aaaacttggc taaattcaaa aaataaatta
       61 aatttgaaac atcttttatc attctaaatt taaacctatg aacaaaaaaa tattgcttac
      121 tttattgtta acatttgtta tctttatttc atgcaaacct gttgaaaaaa agcaaaataa
      181 aaaaatcgat tcaactttac agcctatgga acttaccact tttgacacta tttatttgtt
      241 gcaacccgac acaacaggag gtaagccatt gatgtacgct ttgcaacatc gcaaatcaga
      301 tagagaattt gaacaaaaaa atctgtcatt aaaacatctt tcagaaattt tatgggcagc
      361 ttatggggtt aatcgtccta atggtaaacg aaccgtacct tcagctatgg ctctttatcc
      421 gttgcaagtt tatgctgttt tgtcaaatgg tatttatttc tatgaaccta atcagcatgc
      481 gttaatacct acggtaaaag gtgattatcg ttcgctggct ggattgcagg attttgttta
      541 cactgcacca cttaatctga tttttattgc cgattaccac aagtataata ctgatagaaa
      601 aattcctgat gaaaaccgta ttcgattggt ttcactggat gcggctcact ctactcaaaa
      661 tgtttatttg tattgtgctt ctgaaggtct aaaatgcgta gttcgagcag gtgcaaaaga
      721 aaaagaatta ctcgaagtgt taaaacttga gccaaactat tatcaattta ttgtcgctca
      781 aaccgttggc tattaatgat taaaaaaata aatttaactt cataaacatt cacttattaa
      841 attgaaacaa gattgaaaaa aaccaaaaaa tcaatcttgt tcctttttat aaagtgggta
      901 aatcaatacc atttattttt ttataaaaat ccagtgcata cacatccgtc atccctgaaa
      961 tgtaatcaag aacagccatt attttaccat acatagtagg atgattgatt tcatactgag
     1021 aaggaatacg cattaaaagt tgttgagaat aagccttttg aggttggaga acagcatgta
     1081 ttaagttttc aagcaaggtt aaaataattt tataccccga tagttccact tccaatactt
     1141 gcttagagca ataaatttta ctgtttgcta catccgaaca atattgataa gcccttgcag
     1201 ataaaggtgg caattgttca ataagagaag tatgaaaggt gccatttaaa atattttctt
     1261 cgttatcaac aaaaatttgt gaacattctt ccaccagttt cccaatcaca gaagaacgta
     1321 aataggcaat ttgctcattt tcatcattga ctaaattcaa cacttccatt ttttgtcgtt
     1381 tctgattttc gtcaaaataa ttcaaaaaaa gaaatttcgt ttcttctgtt gaaagaattt
     1441 ttaatttatg agcatcttca atatccatta attgataaca aatatcatca gcagcttcta
     1501 tcagaaaaac taaaggataa cgcacatagt gaaaagaatt actttcaaca tttataattc
     1561 ctaattcatc agcaattttt ttaaaatcgt cagcttcgga attaaaaaaa ccaaattttg
     1621 agtgttgact atttgtgtaa gcagaagcat aaggatattt tacaatagaa gcaagtgtat
     1681 tgtaagtaag tacaaaacca ccttttcgcc tgccagcaaa ctgatggcta agtaatcgga
     1741 aagtatttgc atttccttca aagcaaacca aatctttcca ctccacagga gacacctttt
     1801 ctgccaactt ttgtccttcc ccttcagaaa aaaaagtagc aatagctttt tcgcccgaat
     1861 gaccgaaagg cggattgccc atatcatgnn nnnnnnnngc taaacaagca gcagcgacaa
     1921 ttgcatcaat ttcatgaata attggacaat taggatgaca atcagctaaa cagcgagcta
     1981 catttatacc taatgatcgc ccgacactcg acacctcaag actatgagtc aagcgattat
     2041 gtacaaaaat acttccaggc aaaggaaaaa cctgtgtttt attctgtaat cgacgaaatg
     2101 gaggtgagaa aatcaaacga tcataatccc gttgaaattc agttctgcta tcctttctgg
     2161 gttcgtgata tgattctaat ccaaaacgtt tggttgataa aagttgctcc caattcatca
     2221 taagtcattc aatattatta ggaaatttca tacttgaata tttttttaac tcacttttaa
     2281 gtttattgct gttcccatta gtaactcttt ccaatatttg atagaaatta gttccatggt
     2341 tcatttccac tgtatggcat agttcatgaa gaatcacata atccaccaga tgaataggca
     2401 acaacattaa ataaaaggat aaattaatat ttaaatgatt tgaacaactt ccccagcgcg
     2461 ttttactgga ttgaattttt acttgtttat atttaaagtt atgttgttcg gctaattttt
     2521 gcgtacgttt tggcaaaaaa tgttttgcct cttgacgcaa aaaataaaaa acacctttcc
     2581 acaaatgttt ttgcataact ggttgctgaa aattaaaatt ctctggtacg aaaatgttta
     2641 accaaccatt tcttaattca aaatatatat tttctctttt ttcttctttg atggtgatct
     2701 gaaaagtaag tgtagagaaa acagtatttt tattcaatac aatcacattt ttttcattcc
     2761 tttgctttat caacaattgc tttttgataa tttttggacg aagagaatca ataaatttca
     2821 aggcatcatt ttttgtggaa ttaacaggta aagtaacgcg caagccatca gttaaaattt
     2881 taatatttat gtattttgct ctgttactgt gaacaaaaaa aatttctccc aattctttat
     2941 caatcatata ttaaaatttg agtcgcaaaa taaatgattc cctcactcaa aaaaagaaga
     3001 ttttaaaaac atcga
//
LOCUS       DAOTKS010000199         3014 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_26783_length_3014_cov_3.998648, whole genome shotgun sequence.
ACCESSION   DAOTKS010000199 DAOTKS010000000
VERSION     DAOTKS010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26783_length_3014_cov_3.998648"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..669)
                     /locus_tag="P5236_04670"
     CDS             complement(187..669)
                     /locus_tag="P5236_04670"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HRU63689.1"
                     /translation="MKKEFSVDELFQRAASYCSLSEHCVSEIDQKLTSWKVEKSLKKK
                     LIDKLLANDFINEKRYCKAFVNDKFRFNKWGKIKIALALRAKGLNEELIDEALSFIDE
                     EEYLSILEDLLKSKLATFKNLTDYEKRGKLFRFAQGKGFEPAAIEKVLQYLEENGTFR
                     "
     gene            847..2232
                     /locus_tag="P5236_04675"
     CDS             847..2232
                     /locus_tag="P5236_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRU63690.1"
                     /translation="MELKGLPLTLGTEKIRNLLIQYSLPAIIAMTASSLYNITDSIFI
                     GHGVGALAISGLAITFPFMNLSAAFGSLVGVGAATLMSVRLGQKDYSTANQILGNVFV
                     LNIIFGLAFAIISLIFLDPILYFFGASPATLPYAHDFMVIILIGNVITHIYLGLNGLL
                     RSIGKPEKAMYATLTTVGLNLILNPLFIFGFKWGIRGSALATVLSQTILLIWQIKLFS
                     DKENFIHFQKGIFKLNKRIVKESLAIGMSPFLMNAVGSIIVIVLNQSLIRHGGDLAVG
                     AYGIINRVTFLFAMVVVGLNQGMQPIAGYNFGAQKFDRVNEVLKLTILFATAIMTVGF
                     LIGELFPRAVASIFTKDKELIRLASEGLRITFIFFPIVGFQMVTTNFFQSIGMAGKAV
                     FLSLTRQLIYLLPCLLIFPSIYGLKGVWYSMPVADLLATLTAAYMLFVNYRKNISKSK
                     LISQTKSYQNE"
     gene            2225..2857
                     /locus_tag="P5236_04680"
     CDS             2225..2857
                     /locus_tag="P5236_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HRU63691.1"
                     /translation="MNENFVITIGRQIGSGGHQIGNEIAKYFNIDFYDKELIQAVSKE
                     TGLAKEIFDEMDEKKTHSIFSNFLGLQNSVMMDQFYLCDNYLSNETLFKIQSEVIRKL
                     ADQKSCVFVGRCADYVLKDHPRIVTIFITADFEDRIKRIMERENLSTEKAKEYLKKME
                     KKRADYYNYFSNKQWGAAASYQLCINSSALGVEKTVDYLIEFIKQKLQLE"
BASE COUNT          960 a          488 c          489 g         1077 t
ORIGIN      
        1 atagccacgc ccaatcgcaa aattttatca ttaccaccaa catttgtttt catggttatt
       61 tttttaatga attaatttta attttaaaac aaacttttta taagagaagt tcaataaatg
      121 tataccaaat aataaatgac tttgaaaaat aaaaccgaat caaaaccaaa aaattcattc
      181 ttttgtttaa cgaaaagttc cattttcttc taaatattga agcacctttt caatcgctgc
      241 aggctcaaaa ccttttccct gagcaaaacg aaaaagtttt cctctttttt cataatccgt
      301 caaattttta aaagttgcta actttgactt taacaaatct tccaatatgc ttaaatattc
      361 ttcttcgtca ataaaggaaa gagcttcgtc aatcaattct tcgttcaaac ctttggcacg
      421 caaagctaag gcaattttaa tttttcccca cttgttgaat cggaatttat cattgacaaa
      481 tgccttacaa tatctttttt cattgataaa atcatttgcc agcaatttat caattagttt
      541 tttcttcaaa gatttttcta ctttccagct tgtcaatttc tgatctattt cagacacaca
      601 gtgttctgaa agcgaacaat aagaagctgc tctttgaaat aattcatcaa cagaaaattc
      661 ttttttcata ttcgattact tgtaaaaagc aaacaaaaat aacaaatatt tttctaagtt
      721 tttttattta tcaattgatt agattattta ttctttaaaa acaccttcta taagtcattg
      781 caaaaaacga ttacaacaaa ccagcatttt ttgtaatttt gccacaattt agaagtaaaa
      841 aggcgtatgg aattaaaagg attaccgctc accttaggga ctgaaaagat tcgtaattta
      901 ctgattcaat attcattgcc agccattatt gctatgacgg cgtcgtcgct ttataacatt
      961 actgacagca tttttatcgg tcatggcgtt ggggcattgg ctatatcggg tctggccatt
     1021 actttccctt tcatgaatct ttctgcggct tttggttcgt tggtaggtgt tggagcagcc
     1081 actttgatgt cggttcgttt gggacaaaag gactacagca ctgctaatca aattttagga
     1141 aatgtatttg tattgaatat aatttttggg ttggctttcg cgattatttc tcttattttt
     1201 ttagacccga ttctttattt ttttggtgct agtcctgcca ctttacctta tgcccatgat
     1261 tttatggtta ttattctgat tggcaatgta attactcata tttatctggg cttgaatggt
     1321 ttgctgcgtt cgataggaaa acctgaaaaa gccatgtatg ccaccttaac tacggtaggt
     1381 ttgaacctga tattaaatcc tctttttatt tttggtttta aatggggaat tcgtggaagt
     1441 gctttggcaa ctgttctttc acaaaccatt ttgcttattt ggcaaataaa actttttagt
     1501 gataaagaaa attttattca ttttcaaaag ggtattttta aactaaataa aaggatcgta
     1561 aaagaatcgt tagctattgg gatgtcacct tttttaatga acgcagtggg tagcattatt
     1621 gttattgttt taaatcaatc gttgatcagg catggaggcg atctggcagt tggagcatat
     1681 ggaattatta atcgggtaac ttttcttttt gctatggtgg tagtaggatt aaatcaggga
     1741 atgcaaccca tcgcaggata taattttgga gctcagaaat tcgacagagt aaatgaagta
     1801 cttaagctta ccattctctt tgctactgct ataatgacag taggtttttt aatcggagaa
     1861 ctgttcccca gagctgtagc gtctattttt acgaaggata aagaactcat ccgattagca
     1921 agtgaaggtt tacgaattac ttttattttc tttcccattg ttggatttca aatggtaaca
     1981 actaattttt ttcaaagtat aggaatggca gggaaggcag tttttttatc tttgaccaga
     2041 caattgatat atttattacc ttgtttactc atttttcctt ctatttatgg actaaaagga
     2101 gtttggtaca gcatgcctgt tgctgattta cttgccacac ttacagcagc ttatatgctt
     2161 tttgtcaatt atcgtaaaaa tatttcaaaa tcaaaattaa tttcacaaac aaaaagttat
     2221 caaaatgaat gaaaactttg tcatcaccat tggtcgtcaa attggcagtg gtggtcatca
     2281 aattggaaac gaaatagcaa aatatttcaa tatcgatttt tacgacaagg agctgattca
     2341 ggcagtttca aaggaaactg gtttagcaaa agaaattttt gatgaaatgg atgagaagaa
     2401 aacccatagc attttcagca actttttagg actgcaaaat tctgtgatga tggatcaatt
     2461 ctatttatgc gataattatc tctccaacga aactttattt aaaattcaga gcgaagtaat
     2521 tcgtaagctc gccgatcaaa aatcttgtgt ttttgtaggc agatgtgctg attatgtgtt
     2581 gaaagatcat ccaagaatag tgactatttt tattactgcc gattttgagg atcgaatcaa
     2641 aagaattatg gaacgggaaa atctttccac tgaaaaagcc aaagagtatc taaaaaaaat
     2701 ggagaaaaaa agagccgact actataatta ttttagcaac aaacaatggg gagctgctgc
     2761 ttcgtatcaa ctatgcatca attcttcagc tcttggtgta gaaaaaacgg ttgattatct
     2821 tattgaattt atcaaacaaa aattacaact cgaatgattg atagcttttt agcatgattt
     2881 taaaagcaaa aaattatttt tatccattta aataaaaata attcccttca cttaatgata
     2941 aatagctctg atcattctat catttcctga caaatctttt tttagttcaa tctgctgaaa
     3001 gctttcttgt tcga
//
LOCUS       DAOTKS010000200         2971 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_27244_length_2971_cov_3.735254, whole genome shotgun sequence.
ACCESSION   DAOTKS010000200 DAOTKS010000000
VERSION     DAOTKS010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27244_length_2971_cov_3.735254"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            218..697
                     /locus_tag="P5236_04685"
     CDS             218..697
                     /locus_tag="P5236_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRU63692.1"
                     /translation="MRTFFITDQQEIENIIQQCDVCFVGITDEDGSPYVIPMNFAYRK
                     GEIILHSAPEGKHLRLLQQHPMVCITFCSERKLMYQHPDVACSYSMNSKSVLCKGEVS
                     FIEELSEKEKAMNLFMIKYTGRTFKYSLPALRNVKVWKVPIQEITAKAFGQNFKNKG"
     gene            969..>2971
                     /gene="lon"
                     /locus_tag="P5236_04690"
     CDS             969..>2971
                     /gene="lon"
                     /locus_tag="P5236_04690"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRU63693.1"
                     /translation="MSIQEDHPSNNKLNLGETLYILPLRNMVLFPGVLLPVSVGRAKS
                     LRLVKKARENDLLIGVCAQKDKNIEDPTSEQLYKVGTVAKIVKIFEMNDNTTTVILEG
                     GPRFRIAEFQTEKPFFKAKIEIAEEKIPETDDTVFLALISNIKEVATKIISNSGIMPP
                     ENIFAIRNIDNPVFLINYICVNFGLNMEDKQRLLEIDDIKERGYQLLDLLNKELQLLE
                     IKISIQNKAKEGIDKQQREYFLQQQLKTIQDELGGTSPEEDIVEFRERAKQKKWNESV
                     AKVFNKELNKLERMSTYSPDYSIQLNYVEMMLDLPWNEYTKDNFNLKNARKILDKDHF
                     GLETVKERILEHLAVLKLKGDMKAPILCLYGPPGTGKTSLAKSIAVALNRKYVRVSLG
                     GLHDEAEIRGHRRTYIGALPGRIIQNIQKAQSANPVFVLDEIDKVSADYKGDPSSALL
                     EVLDPEQNSAFHDNYLDVDFDLSKVLFIATANNINTIPLPLLDRMELIEVSGYTQEEK
                     IEIARRHLIPKELENHGLTKEQFILPNPVIGKLIENYTRESGVRELDRQIASLMRKVA
                     KKVAANEPYNPQLSEEDLVTYLGAPRYTKDKYEGNEYAGVVTGLAWTQVGGEILYIEC
                     SLSKSKSPHLTLTGNLGEVMKESAIIALQYIKAHAEKLGIPNE"
BASE COUNT         1110 a          429 c          554 g          878 t
ORIGIN      
        1 taattattta aagatgagac aaaaaaagtg ccatttttga tgaataaaaa taaacaaacg
       61 ttgaaaaatt atcatagcac aggatttgaa attcacaaat tataaaagca cgggctataa
      121 ttttgacaaa gcaaggttta aagaaaatac tcaaaaacta aaaagataac aaatccaaac
      181 aaccttttaa gtatatttgt caaataatta aagaaatatg agaacatttt ttatcactga
      241 tcaacaagag attgaaaata tcattcagca atgtgatgtt tgttttgtag gcattaccga
      301 tgaagatgga agtccctatg ttatcccaat gaattttgct taccgtaaag gtgaaatcat
      361 tttgcattct gctcctgagg ggaaacattt acggttactc cagcagcatc caatggtttg
      421 cattactttc tgttcagaga gaaaattaat gtatcaacat cccgatgtgg cttgtagtta
      481 cagtatgaat tcaaaaagtg ttctctgcaa aggtgaagta agctttattg aagaactttc
      541 tgaaaaagag aaagcaatga atctctttat gatcaaatat acaggacgaa ctttcaaata
      601 ttcgcttcct gctttacgaa atgtaaaagt ttggaaagta cccatccaag aaataacagc
      661 aaaagcattt ggccaaaatt ttaaaaacaa aggataaaca ttttttgtta ttttctgata
      721 aatacaataa aagcagagcc aaagagaatg acaaattgtt gatttttggc ataactattg
      781 agaaataaag aactgctgtg aaatatcaaa aaatctaagt tttttacaat aaaattttga
      841 tagtgtaaaa cacagaggaa ataattgtgt cattttgtca tataaaaatt aaaacgataa
      901 gataagaaat gaattatata tttgatgatt ttgaagaatt agaaaattca tcagaaattt
      961 tcccgattat gagtattcaa gaagatcatc catccaataa taaattaaac ttaggagaaa
     1021 cactctatat ccttccgttg agaaatatgg tactttttcc gggtgtttta ttgcctgttt
     1081 cagtaggacg ggctaaatct ttacgattag tcaaaaaagc cagggaaaac gacttgctaa
     1141 ttggtgtatg tgctcaaaaa gataaaaata ttgaagatcc tacttctgaa caactttata
     1201 aggtgggaac agtagcaaaa attgttaaaa ttttcgaaat gaatgacaac accactaccg
     1261 ttattctgga aggaggacca cgttttcgga tcgccgaatt tcagacagaa aaaccttttt
     1321 tcaaagcaaa aattgagata gcagaagaaa aaatacctga aactgacgat actgtttttt
     1381 tggcattgat ttccaacata aaagaggtgg cgacaaaaat tatttctaat tcgggcatca
     1441 tgccacctga aaatattttt gctattcgca atattgataa tccagtattt ttaatcaatt
     1501 acatttgtgt caattttgga ttgaatatgg aagataagca aagattgctt gaaattgatg
     1561 atattaagga aagaggttat caattgctgg atttgttaaa taaggaatta caattgctgg
     1621 aaataaaaat ttctattcaa aataaggcaa aagaagggat tgataaacaa caaagagaat
     1681 attttcttca acaacagtta aaaactattc aagatgagtt aggaggtact tcaccagaag
     1741 aagatattgt tgaattcaga gaacgtgcaa agcaaaaaaa gtggaacgaa tcggtggcta
     1801 aagtttttaa taaagaactc aataagttag aacgaatgag tacttattct cctgattact
     1861 ccattcagtt gaattatgtt gaaatgatgc tcgatttgcc gtggaatgaa tatacaaaag
     1921 ataattttaa tttgaaaaat gctcgtaaaa ttcttgacaa agatcatttt ggactggaaa
     1981 ctgtgaaaga acgaattctg gaacatctgg cagtattgaa actgaagggt gatatgaaag
     2041 ctcctattct ttgtttatat ggaccaccgg gaacagggaa aacttcattg gcaaaatcaa
     2101 ttgcagtggc tttgaataga aaatatgtcc gtgtttcatt gggcggatta cacgacgaag
     2161 ccgaaataag aggccatcgc cgaacttata ttggagcact gcctggacgc atcattcaaa
     2221 atattcaaaa agcacaatct gctaatcctg tatttgtatt ggatgaaatt gataaagttt
     2281 cagccgatta taaaggcgat ccttcttcgg ctttgttaga agtgctcgac cctgaacaaa
     2341 atagtgcttt ccacgataat tatctggatg ttgattttga tttgtcaaaa gtgctattta
     2401 ttgctactgc caacaatatt aatactattc cgcttccatt gttggataga atggaactga
     2461 ttgaagtaag tggatacaca caggaagaaa aaatagaaat tgctcgacgc catctgattc
     2521 caaaagaact tgaaaatcat ggtttaacaa aagaacaatt tattttgcct aatccagtta
     2581 ttgggaaatt aattgaaaat tataccagag aatcgggtgt gcgagaatta gacaggcaaa
     2641 ttgctagcct tatgcgaaaa gtggctaaaa aagtggctgc taatgagcct tataaccctc
     2701 aattgtcaga ggaagatttg gtaacatatt taggggctcc acgttatacc aaagataaat
     2761 atgaaggcaa tgaatatgca ggcgttgtaa caggtttagc atggacgcag gtaggcggtg
     2821 aaattttata tatcgaatgc agcttgagta aaagtaaatc tccgcatttg acgttaacgg
     2881 gaaatttggg cgaagtgatg aaagaatcag ctattattgc tttacagtac attaaagcac
     2941 atgctgaaaa attaggcatt ccaaacgaaa t
//
LOCUS       DAOTKS010000201         2970 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_27252_length_2970_cov_6.032590, whole genome shotgun sequence.
ACCESSION   DAOTKS010000201 DAOTKS010000000
VERSION     DAOTKS010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27252_length_2970_cov_6.032590"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="P5236_04695"
     CDS             <1..318
                     /locus_tag="P5236_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63694.1"
                     /translation="GNYVKNILAFFVIFALIWVIKNKVWRNYLLLGSFTIGYLLVMAF
                     SAFAQSERFHQPALPFILILAAVGVSKSTNKTKKYFLWWLGFIFVAIVGWNWFKLAGR
                     GLT"
     gene            470..1402
                     /locus_tag="P5236_04700"
     CDS             470..1402
                     /locus_tag="P5236_04700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRU63695.1"
                     /translation="MRILFVCSKNSGKIAPFIQEQAEAIQQMGTEVDFFTIEGKGWKG
                     YLKNRPFLTKKIKEFKPNVIHAHYGLSGLLANAQRKVPVVTTYHGSDINDPKVFRFSK
                     WAMRLSAWNIFVSEKNIRIAKVKKKYTLIPCGINTELFKPIEKQVARKKFGFSTNEKL
                     VLFSGSFDNPVKNSALALQAINLLPEVQLLELKNYSREEVALLINAVDVCLMTSLSEG
                     SPQFIKEAMACNCPIVSVDVGDVKEQIEDVENCFIVEAQCEKIANKINGILADGAKRT
                     NGREKLLRKGLEQSSVSEQLIKIYRRYGGTATSK"
     gene            1377..2378
                     /locus_tag="P5236_04705"
     CDS             1377..2378
                     /locus_tag="P5236_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HRU63696.1"
                     /translation="MVELLQANEIETDQWEQLVENSPVASFFQTPECYQFYASLTFLK
                     PFLYGVAEDGKLKGIACGYVQAEGGKLKQFFSRRAIIPGGILLDKDIEPGSITALLEF
                     LKKELHKRAIYIECRNFNDYSEYRPYFEKVGFIYQAHLNFHVPTPNVEQCLKKIKSTK
                     RRYIKLSLKEGAEITETKDENDIKNFYQLLNNLYRTKIKTPLFPYEFFENIVRLPFGH
                     LFVVKYQKEIIGGNLCVALDNKILYEWFVCGLDGEYKKIFPSTLATWSAIQFAAENGF
                     EYLDLMGAGKPDESYGVREFKAEFGGNLVEHGRFEMILNKYLYHVGIISVKFLKHWK"
     gene            2375..2836
                     /locus_tag="P5236_04710"
     CDS             2375..2836
                     /locus_tag="P5236_04710"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRU63697.1"
                     /translation="MNINLWFIRIKNKLIYSCGVHFPYSKVRRKSLNWLGYQVGKNVY
                     FPGDLIITQNFTGDRGKLIIGDNVSIGPRCALILFSHPNFSQIRNQLSEKKREIVIEK
                     DAWLGAGVIVLPGVKIGEGAIIGAGAVVTKDVEPFTIVGGVPAKKIGEVLH"
BASE COUNT         1031 a          429 c          617 g          893 t
ORIGIN      
        1 ggaaattatg taaaaaatat tttggctttt tttgttatct ttgctttaat atgggtaata
       61 aaaaataaag tttggcgcaa ctatttgttg ttaggttcgt ttaccatcgg ttatttgttg
      121 gtcatggctt ttagcgcttt tgcccagtcg gagcgatttc atcagccagc tttgcctttt
      181 attctgatat tggcagccgt tggagtgagt aagtcgacga acaaaacaaa aaaatatttc
      241 ctttggtggt tgggatttat ttttgtagca attgttggct ggaattggtt taaactggca
      301 gggagaggat tgacgtgaaa aaatgaggag tattaagttt agagtatgaa gtgtagagta
      361 ttgagtgtag aattgggagt agaaaattcc gaattctcat aactcataac ttaaaactca
      421 tgactcggaa cccaggactt aaaaacgaaa ttatgaatga cgaattaaca tgagaatatt
      481 atttgtttgc agcaaaaatt caggaaaaat cgcgccattt attcaggaac aagccgaagc
      541 cattcaacaa atgggtacgg aagtggattt tttcaccatc gaaggaaagg gctggaaagg
      601 ctatttaaaa aaccgacctt tcttgacgaa aaaaatcaag gaatttaaac cgaatgtgat
      661 tcatgctcac tacggtttgt cagggttact ggctaatgca caacgaaaag ttccagttgt
      721 tactacttat cacggaagcg acatcaacga tccaaaagta tttcgttttt caaaatgggc
      781 aatgcgatta tcggcatgga atatctttgt gtcagaaaaa aatatacgga tagcaaaagt
      841 aaaaaaaaaa tataccttga taccttgtgg tataaacaca gaacttttta aaccgattga
      901 gaaacaagta gcgcgtaaaa aatttggatt ttcaactaat gaaaagttag tattgttttc
      961 gggaagtttc gacaatccgg taaaaaattc ggctttggct ttacaagcta tcaacctttt
     1021 acctgaagtt cagctgctgg aattgaaaaa ttacagccgt gaagaagtgg ctttgttaat
     1081 aaatgctgta gatgtttgtc taatgaccag cctcagtgaa ggttcaccac aatttatcaa
     1141 ggaagcaatg gcttgcaatt gcccgattgt aagtgtagat gtaggtgatg tgaaagaaca
     1201 aatcgaagat gttgaaaact gttttattgt tgaagctcaa tgtgaaaaaa ttgcaaacaa
     1261 aataaatgga atccttgcag atggtgctaa aagaacaaac ggacgagaaa aactgctaag
     1321 aaaaggattg gaacaatctt cggtatcaga acagttaata aaaatttatc gtcgatatgg
     1381 tggaactgct acaagcaaat gaaatagaga ctgaccaatg ggaacaactg gttgaaaatt
     1441 ctccggttgc ttcctttttc cagacgccgg agtgttatca gttttacgca tctctgactt
     1501 ttctgaagcc ttttttgtat ggagtagcag aagatggaaa gttaaaagga attgcttgtg
     1561 ggtatgtgca ggctgaaggg ggaaaattga aacagttttt cagcagacgg gctattattc
     1621 ccggcggaat tttattggat aaggatattg aacctggctc gataacagca ttgctcgaat
     1681 tcctaaaaaa agaacttcat aaaagagcca tttatattga atgcagaaat ttcaacgatt
     1741 atagcgaata tcgcccttat tttgaaaaag ttggatttat ttatcaggct catttgaatt
     1801 ttcatgttcc aactccaaac gttgaacaat gcttaaaaaa aataaaaagt acgaaaagaa
     1861 gatatattaa actttcttta aaagaaggtg ctgaaattac ggaaacaaaa gacgaaaacg
     1921 atataaaaaa tttttatcaa ctcttgaaca acttatatcg gacaaaaata aaaacacctc
     1981 ttttcccata tgagttcttt gaaaatattg tgcgtctacc ttttggacat ttgtttgtcg
     2041 tcaaatatca aaaagaaatt atcggaggaa atttatgtgt agctttggat aataaaattc
     2101 tttacgaatg gtttgtttgc ggattagatg gagaatataa aaaaatcttt ccttctactt
     2161 tggcaacatg gtcagccata caatttgctg ccgaaaatgg gtttgaatat ctcgacttga
     2221 tgggcgcagg aaaacctgat gaaagttatg gcgtaagaga atttaaagca gaatttggag
     2281 gaaatctggt ggagcatgga agatttgaaa tgattctcaa taaatattta tatcatgttg
     2341 gaattatcag cgttaaattt ttaaaacatt ggaaatgaat ataaatttgt ggtttatccg
     2401 aataaaaaat aagcttatct actcttgtgg ggtacatttc ccatatagta aagttcggag
     2461 aaagtcctta aattggttag ggtatcaagt aggaaaaaat gtttattttc ccggtgactt
     2521 gattatcact caaaatttta ctggagatag aggcaaattg attattggtg ataacgtatc
     2581 aattggacct cgctgtgcgc tgatattgtt ctcacatcct aatttttcgc aaataagaaa
     2641 tcaattgagt gaaaaaaaaa gagaaatcgt cattgagaaa gatgcttggt taggagcagg
     2701 tgtgattgta ttacccggtg tcaaaattgg tgaaggagcc atcataggtg caggtgcagt
     2761 ggttacaaaa gatgtggagc cttttaccat agttggtgga gtgccagcaa aaaaaattgg
     2821 agaagtattg cattaaaatc taagcaaatt cgttaaaaaa ttacatcctg ccaaaaataa
     2881 aacaaaaaat gaatttactg attgatataa cgcatccggc tcatgtacac ttaaccaaaa
     2941 atgttcattc tttgttgaca gacaaaagac
//
LOCUS       DAOTKS010000202         2885 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_28217_length_2885_cov_3.806360, whole genome shotgun sequence.
ACCESSION   DAOTKS010000202 DAOTKS010000000
VERSION     DAOTKS010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28217_length_2885_cov_3.806360"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="P5236_04715"
     CDS             <1..380
                     /locus_tag="P5236_04715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRU63698.1"
                     /translation="PQNFFKKRKEHFEIRASGYNKIMDVPYLSGCFMFLRMEALKQVG
                     LFDERFFMYPEDLDLTRRIHQKYRTVYYPEVSIVHQHVQSSYENLRMLWIHIINMIKY
                     FNKWGWFCDKERKEVNQEIMKQL"
     gene            377..958
                     /locus_tag="P5236_04720"
     CDS             377..958
                     /locus_tag="P5236_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HRU63699.1"
                     /translation="MNISLLQIASAFMVLFAIIDILGSIPIILGIKSKGESVSPAKTS
                     LVAFVILVVFLFAGEWVLKLFHVDIESFAAAGSLIIFIFSLEMILDVEIFRNKGPEGV
                     SSIVPIAFPLIAGPGSFTTLLSLRAEYEMINILIALTLNIIFVYFVLKSTDKVEKIIG
                     KGGIYILRKFFGIILLAIAVRLFATNIKSLLAY"
     gene            complement(969..2138)
                     /locus_tag="P5236_04725"
     CDS             complement(969..2138)
                     /locus_tag="P5236_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRU63700.1"
                     /translation="MNQQRYHLRGVSSSKEDVHDAIKNIDKGLYPKAFCKIIPDMLGN
                     DSDYCNIMHADGAGTKSSLAYVYWKETGDLSVWKGIAQDALIMNIDDLLCVGAVNNIL
                     VSSTICRNKNLIPGEVISTIINGTDEIIQQLCEMGINVYFTGGETADVGDLVRTIIVD
                     STVACRMKRADIIDNANIKTGDVIVGLSSSGQAIYEKEYNSGIGSNGLTSARHDVFVH
                     YLAEKYPESYDPAMPDDLVYTGSYSLTDKIESLQLDAGKLVLSPTRTYAPVVKKILEE
                     LHPYIHGMIHCTGGGQTKILHFIDQLRVIKNNLFPLPPLFQIIQKESGTDWKEMYKVY
                     NMGHRFEIYLPQEVAQNVIDIARTFHIDAQIIGYCEASEKKELIIESEFGKLKYK"
     gene            complement(2196..2396)
                     /locus_tag="P5236_04730"
     CDS             complement(2196..2396)
                     /locus_tag="P5236_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63701.1"
                     /translation="MKLKSLLFALGVFVTYLVIVVLLRLYTKQIPENAVILNFFSKND
                     FFIGLLVTFFVTLAHEQKKRLK"
     gene            complement(2443..>2885)
                     /locus_tag="P5236_04735"
     CDS             complement(2443..>2885)
                     /locus_tag="P5236_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRU63702.1"
                     /translation="FFENQPSILYKLKTLQDVGLGYIKLGQPSTTLSGGESQRVKLSA
                     ELSKRDTGKTLYILDEPTTGLHFEDVRVLLGVLNRLVDKGNTVIIIEHNMDVIKTADY
                     IIDLGPAGGEAGGKIICTGTPQEVANHPKSETGKFLKLELKSLI"
BASE COUNT          909 a          494 c          457 g         1025 t
ORIGIN      
        1 ttcctcaaaa tttctttaaa aaaaggaagg aacattttga gatacgtgcg tcaggatata
       61 ataaaataat ggacgtacct tatctgtcag gttgctttat gtttttaaga atggaagctc
      121 taaaacaagt tggtttattt gatgaacgat tttttatgta tcctgaagat cttgatttga
      181 cacggagaat ccatcaaaaa tatcgaacgg tttattatcc tgaagtgagc attgttcatc
      241 aacatgttca aagttcttat gaaaatttac gaatgttgtg gattcacatt attaacatga
      301 taaaatattt caataaatgg ggttggtttt gcgataaaga gagaaaagaa gtgaaccagg
      361 aaattatgaa acagctatga atattagttt attgcagata gcaagtgcct tcatggtact
      421 ttttgccatt attgatatat tgggatccat tccaattatt ttgggtataa aaagtaaagg
      481 agaaagtgtt tcgcctgcta aaaccagttt agtagcattt gttattttgg ttgttttttt
      541 gtttgctggg gaatgggttt taaaactttt tcatgttgat attgaatcat ttgctgcagc
      601 cggttccttg attattttta tcttttcact ggaaatgatt ctggatgttg aaatttttcg
      661 aaataaaggt cctgagggcg tctcctccat tgttcctatt gcttttcctt taattgctgg
      721 tcctggctcg tttaccactt tactttcttt acgtgctgaa tatgaaatga tcaatatcct
      781 cattgccctc actttaaata ttatttttgt ttattttgtg ttaaagtcaa ctgataaagt
      841 ggaaaaaata attggcaaag ggggtattta tattttacgt aaattttttg gaatcatttt
      901 attagcgata gctgttagac tatttgcaac aaatattaaa tcgttactgg cttattaatt
      961 tgttggtctc atttatactt aagctttccg aattcgcttt caataattaa ttcttttttt
     1021 tcagaagctt cacaatatcc aattatttgt gcatcaatat gaaaagttct tgcaatgtca
     1081 attacatttt gtgcaacttc ctgcggtaga tatatttcaa atcgatgtcc catattataa
     1141 actttataca tttctttcca gtctgttcct gattcttttt gaataatttg aaaaagtgga
     1201 ggtaagggga acaaattatt tttaatcact ctgagttgat caatgaaatg taaaatttta
     1261 gtttgtcctc ctcctgtaca atgaatcatt ccatgaatgt aaggatggag ttcttctaaa
     1321 atttttttta ccacaggagc ataggtccga gtgggagaaa gtaccaactt acccgcatcc
     1381 aattgtaaag attcaatttt atctgttaat gaataagagc cagtataaac caaatcatct
     1441 ggcatagcgg ggtcataact ttctgggtat ttttctgcaa gataatgaac gaacacatcg
     1501 tggcgggctg atgttaagcc attgctgcct atgccactgt tgtattcctt ttcgtaaatt
     1561 gcctgtccac ttgaagaaag tcccacaatc acatcgcctg tttttatatt ggcattatca
     1621 ataatatcag ctcttttcat ccggcaagca acggtactat ccacaataat agtgcgaacc
     1681 aaatcaccta catcagccgt ttctccacca gtgaaataaa catttattcc catttcgcac
     1741 aactgctgaa taatttcatc tgttccattg attatggttg aaataacttc gccaggaatc
     1801 agatttttat ttctacagat cgttgatgaa accagaatat tatttactgc tcctacgcac
     1861 aacaaatcat caatattcat aatcaaagca tcctgagcta ttcctttcca tacggacaaa
     1921 tcaccagttt ctttccaata aacatacgct aaagaggatt tagttccagc cccatcagca
     1981 tgcataatat tacagtaatc ggaatcattc cccagcatat caggaataat tttacaaaaa
     2041 gcttttggat acaagccctt gtcaatattt tttatagcat cgtgcacatc ttcttttgag
     2101 gaagaaacgc ctcttaaatg gtatcgttgt tggttcatgt aaaatttatt aaaaaatatt
     2161 ttgaaattat ttttaaataa ttgaaagcta attaattatt tcaatctttt cttttgttca
     2221 tgagcgagcg ttacaaaaaa ggtcaccagc aagccgataa aaaaatcatt ttttgaaaag
     2281 aaattcaaaa taaccgcatt ctcagggatt tgttttgtat ataaacgtaa taaaacaact
     2341 attaccaaat aagtaacaaa tactcctaag gcaaataaca aagattttag tttcataata
     2401 aaaatttcct aatttgaatg aagccaaaga taataaaaat aactaaatta agcttttcaa
     2461 ttccaatttc agaaatttac ctgtttcact tttgggatgg tttgccactt cctgaggagt
     2521 acctgtacaa ataatttttc cgccagcttc tcctcccgct ggtcctaaat caatgatata
     2581 atcggctgtt ttaattacat ccatattgtg ttcaataata ataacggtgt ttcctttatc
     2641 aaccagtcta ttcaaaactc ccagtaatac tctaacatct tcaaaatgta aaccggtagt
     2701 aggttcatcc aaaatatata aagtttttcc tgtatctcgt tttgaaagtt cggcactcaa
     2761 ttttactctt tgactttcgc cgccagaaag tgtagtgctg ggctggccca gtttaatata
     2821 tcctaatcct acatcctgta aagttttaag tttgtataaa atagaaggtt gattttcaaa
     2881 aaaat
//
LOCUS       DAOTKS010000203         2871 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_28419_length_2871_cov_5.302557, whole genome shotgun sequence.
ACCESSION   DAOTKS010000203 DAOTKS010000000
VERSION     DAOTKS010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28419_length_2871_cov_5.302557"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..318
                     /locus_tag="P5236_04740"
     CDS             25..318
                     /locus_tag="P5236_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRU63703.1"
                     /translation="MLKNTKQIEEAAYLLKAIANCTRLGIILLLSEKDELNVSQLQEE
                     LNCEQSLLSHHLTDMRAKGILNCRRESKNCYYSVKNKKIVQVIECILSCQNQN"
     gene            519..998
                     /gene="trxA"
                     /locus_tag="P5236_04745"
     CDS             519..998
                     /gene="trxA"
                     /locus_tag="P5236_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRU63704.1"
                     /translation="MKKFIQLSLILALITFNACAGNNKEKTQKNNYTKEKTMGTIHLT
                     KEEFLKKVVNYETNPNEWKYLGDKPCIIDFYADWCGPCKVIAPILEELANEYNGQIYI
                     YKINTDKEQELSAAFGIRSIPTLLFCPLKGDPHMALGAMPKESFVKAINEILLKKQN"
     gene            1212..>2871
                     /gene="sppA"
                     /locus_tag="P5236_04750"
     CDS             1212..>2871
                     /gene="sppA"
                     /locus_tag="P5236_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HRU63705.1"
                     /translation="MKQFFKFTLASILGVLIASLLSFIIFFSIISGLASSKEKAPKLK
                     PNSIYQLNLKGELVERSQEEPFSVALSQAFGKSDYQTLGLDDILKNIQKAKNNPDIVG
                     IYLNCGPMVAGFASLKEIRDALVDFKKSGKFIIAYADNYSQKIYYLASVADKVLLNPQ
                     GMLDWKGLSAQVVFFKNTLDKLGVEMQVVKVGTFKSAVEPYVNTKMSDANRQQVTEYT
                     QALWNTVLSEVSLSRKIPVDKLNAYADELITFQPAEKTLEYQLIDSLLYVEQVENLLK
                     SLIKDTTLKELNYVKHSAMCKVPENVKFERNKVAILYGVGEIDMESRGGIQSEEFVKT
                     INKIAKDNSIKAVVFRVNSPGGSAYASEQIWHALTLLKEKKPLIVSMGDYAASGGYYI
                     SCMADSIVAQPNTLTGSIGIFGLIPNVKGLNDKIGLTFDGVKTNKLSDAISVNRPFTP
                     EEKDLMQNYVNRGYELFVKRCADGRRMKIDQIKAIAEGRVWSGKEALKIGLVDKIGGI
                     DEAVMMAAKKANLISYQIVEYPKKESFAEKIMKSFSSHAETHFIK"
BASE COUNT         1100 a          396 c          498 g          877 t
ORIGIN      
        1 tgtacttgaa tttaaataag aaaagtgttg aaaaatacaa aacaaataga agaagctgct
       61 tacttgctta aagcaatagc caattgcact cgtttgggta ttattctatt actttcagaa
      121 aaagatgaat taaacgtgtc gcaacttcag gaagaattaa attgtgaaca gtcgttgctt
      181 tctcatcatc tgacagatat gagagcaaaa gggatattaa attgcaggcg agaaagcaaa
      241 aattgttatt attccgtgaa aaacaaaaag atagtgcaag taattgaatg tatcctctcc
      301 tgtcaaaatc aaaattaaaa agaaagaaaa agctaaggac gtttttaaaa aaatacttta
      361 tggaacaatt ggcgattttt attgtttttt atctttccgc agaaagaaaa aacaaaaaat
      421 aaaaatgtta ttaataattg tttgcaattg ctttattagt agcaaaaaaa ataattatct
      481 ttacaaggaa aaaacttaaa aaatcaaaca agagaaatat gaaaaaattt attcagctaa
      541 gtcttattct ggcattaatc acttttaatg catgcgctgg caacaataaa gaaaaaacac
      601 aaaaaaacaa ttacacaaaa gaaaaaacga tgggaacaat tcatttaaca aaagaagaat
      661 ttttaaaaaa ggtagttaat tacgaaacca accctaacga gtggaaatat ttaggggata
      721 aaccttgtat tatagatttt tatgcagatt ggtgtggacc ctgtaaagta attgctccta
      781 ttttggaaga attggctaat gaatataatg gacaaattta tatttacaaa ataaatacag
      841 ataaagaaca agaactgtcg gcagcttttg ggatccgcag cattccaaca ttacttttct
      901 gccctctgaa gggagatcct catatggctt taggtgcgat gccaaaagaa tcgtttgtaa
      961 aagctattaa tgaaattctg ttgaagaaac aaaattaaac tcattttgaa agataattga
     1021 taaaatgttc aatatcaaaa gaaaaaacag ttaataagta agaaaatagg tggattttat
     1081 cgttagcaca catttataaa aatttgttag agatattatt cccaaaaaat cgtttcagtt
     1141 gttaaaaagt aaaaaatatt ctcttttttg taagctaaca actttaataa atctatatta
     1201 aacaaaacat tatgaaacaa ttttttaaat tcacacttgc ctctatttta ggagtgctta
     1261 ttgcaagttt gctctctttt atcatttttt tcagtattat tagtggatta gcaagctcaa
     1321 aggaaaaagc acccaaatta aaaccaaatt ctatctatca attgaattta aaaggtgaat
     1381 tggtcgaacg atcacaagaa gaaccttttt cagttgcttt atcacaagct tttggaaaaa
     1441 gtgattacca aactttggga ttagatgata ttttaaaaaa tatccaaaaa gcaaaaaata
     1501 atcctgatat tgttggaata tatttgaatt gtggaccaat ggtagctggc tttgcatctc
     1561 taaaagaaat tcgggatgct ttagttgatt ttaaaaaatc cggaaaattt attattgcct
     1621 atgctgataa ttattctcaa aaaatatatt atttagcgtc ggtagccgac aaagttttat
     1681 taaatccaca aggcatgctt gattggaaag gattgtccgc acaagtagtt ttctttaaaa
     1741 acaccttaga taaattaggt gttgaaatgc aggtagttaa agtcggaact tttaaatcgg
     1801 ctgttgaacc ttatgtaaat accaaaatga gcgatgccaa tcgccaacaa gttaccgagt
     1861 acacacaagc tctttggaat acagttttat cagaagtgtc attatcccgt aaaatcccag
     1921 tagataaatt aaatgcttat gcagatgaat tgataacttt tcagcccgcc gaaaaaactt
     1981 tagaatatca actaattgac tcattattgt atgttgagca agtagaaaac ctgctgaaat
     2041 ctttgataaa ggataccact ttaaaagaac tgaattatgt gaagcactct gctatgtgta
     2101 aagttccgga gaatgtaaaa tttgaaagga acaaggtggc tatcctctat ggagtaggag
     2161 aaattgatat ggagagtaga ggaggcattc aatcagaaga atttgtaaaa actattaata
     2221 aaatagcgaa agacaattcc attaaagccg ttgtttttag agtaaattcc ccaggaggaa
     2281 gtgcctatgc ttctgaacaa atatggcatg ctctcactct attaaaagaa aaaaagccat
     2341 tgatcgtatc tatgggtgat tacgctgctt caggaggata ctatatttca tgtatggctg
     2401 atagtattgt agctcaacct aatacactaa caggttcaat tggaattttt ggtttgatac
     2461 ctaatgtaaa aggactgaat gataaaatcg gtttaacttt cgatggcgtc aaaaccaata
     2521 agttgagcga tgctatttct gtaaatcgcc catttactcc tgaagaaaaa gatttaatgc
     2581 aaaattatgt caaccgaggt tatgagttat ttgtcaaacg ttgtgctgat ggtcgaagaa
     2641 tgaaaattga tcaaattaaa gcaattgcag agggtagagt ttggtcaggt aaagaagcat
     2701 taaaaattgg gttggtagat aaaattggcg gtattgacga agctgttatg atggctgcta
     2761 agaaagcaaa tctaatttct tatcaaatag ttgaatatcc taaaaaagaa agttttgccg
     2821 aaaaaataat gaaaagtttt tcttctcacg ctgaaacaca ttttataaaa t
//
LOCUS       DAOTKS010000204         2857 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_28604_length_2857_cov_4.928622, whole genome shotgun sequence.
ACCESSION   DAOTKS010000204 DAOTKS010000000
VERSION     DAOTKS010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28604_length_2857_cov_4.928622"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="P5236_04755"
     CDS             <1..336
                     /locus_tag="P5236_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRU63706.1"
                     /translation="SIFLLICFLAYIFFCAGSISAVIFVFLSEMYPTRIRGLAMSIAT
                     LSLWIGTYLIGQLTPWMLETLTPSGTFFLFAAMCIPYMLIVWKLMPETAGKSLEDIER
                     FWLKNTKTK"
     gene            398..1513
                     /locus_tag="P5236_04760"
     CDS             398..1513
                     /locus_tag="P5236_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007173589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 domain-containing protein"
                     /protein_id="HRU63707.1"
                     /translation="MKTKKIDRRNFIKNIAIGSVGLLTAQTLNAHVKSEITKKPLFPQ
                     KKSPPLKKLSNEMFKTPGIFKGMPIHATFLDEISWDIPHQNWGVKEWDKDFQAMKAIG
                     IHTVVLIRSGLGRWIASPFKSILDSEDVFYPPVDLVEMFLTLADKYNMAFYFGMYDSG
                     KYWIEGAFQKEIDLNLKLIDEVWEKYRHHTSFKGWYLSQEISRRTKNMSKVYAEIGKH
                     AKAISNNLPTLLSPYIHGIKTDQVMSGDKPITIQQHENEWNEILSNIEGAVDILAFQD
                     GQVDYHELPDYLEVNKKLADAHKMRCWTNIESFDRDMPIRFLPIKWEKLLLKLEAARK
                     AGMEDAITFEFSHFMSPNSTYLQAKYLYDRYKEHFNF"
     gene            complement(1540..2808)
                     /locus_tag="P5236_04765"
     CDS             complement(1540..2808)
                     /locus_tag="P5236_04765"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRU63708.1"
                     /translation="MSDARLNIESLVDLLVEEVQTSRREAEEFFKAFFAALESALLDG
                     ETVTIKNFGIFQVVRHGPHKILNFQNRREFVIPRRCKVIFTPDEILRDLVNKPFSYLE
                     PVELDVEDKDENFVSEEPPLEPLSIFNEQATEIKSLLEEIKNLSPPSYSSLQNEAVEN
                     VPMKEKKESSEQEKEEENIPEEIIEEKNEEILVSYNNEEQEEIVSPPSIISSKNEKDN
                     FDESYKNENLEPLGIPSRPFKGDNLSDIPSPRKRRHFWLPLVLFIIIAGGVSLYYFYS
                     PVNEYVNTLLKIKTDKNLVLSGNKNFITEQPFVTKDSLGEVSKQSANEVDSLQILFDT
                     PRYYSEFITTEKIMEGSRLAGLSRRYYGVPVFWVYIYEANRDRITHPDNIPVGTPIRV
                     PKLDSRLIDTSNPRCLEKAKELEKLYLGKK"
BASE COUNT          952 a          494 c          444 g          967 t
ORIGIN      
        1 agtatttttt tgttaatatg cttccttgcc tacatatttt tttgtgctgg ctctattagc
       61 gccgtcattt ttgtttttct atctgaaatg tatcctactc gcattagggg attggctatg
      121 tctattgcaa ccctttcgtt atggattggc acctatctta tcggacaact tactccatgg
      181 atgttagaaa ctttgactcc atcaggaact ttcttccttt tcgcagcaat gtgcatacca
      241 tacatgctca tcgtttggaa actgatgccc gaaacagcag gaaaatcatt ggaagacatt
      301 gaacgttttt ggttaaaaaa tacaaaaacg aaataaaaaa tttatttcaa caagcattta
      361 caaaaataac ctttagtata aaaattgaaa taaaggtatg aaaactaaaa aaattgatag
      421 aagaaatttt ataaaaaata tagctattgg ttctgtagga ttattaacag ctcaaactct
      481 caatgctcac gttaagtcag aaattacaaa aaaaccatta ttcccacaga aaaaatctcc
      541 acccttaaaa aaactatcca atgaaatgtt taaaacccca ggaattttta aaggaatgcc
      601 aatccacgct actttccttg atgaaataag ctgggatata cctcatcaaa attggggagt
      661 aaaagaatgg gacaaagatt ttcaagcaat gaaagcaatt ggaatccaca cagtagtatt
      721 gattcgttca ggcctgggtc gttggatcgc ttcacccttt aaatctattc tggattcaga
      781 agatgttttt tatcctcctg tagatttagt tgaaatgttt ctgaccttag ctgataaata
      841 taatatggct ttttattttg gcatgtatga ttctggaaaa tattggatcg aaggtgcttt
      901 tcaaaaagaa atagatttaa atttaaagct gatagatgaa gtatgggaaa aatatcgtca
      961 tcatacttcc tttaaaggtt ggtatctttc tcaagaaatt agtaggcgaa ctaaaaacat
     1021 gtctaaagta tatgctgaaa ttgggaaaca tgcaaaagca atttcaaaca atcttcccac
     1081 cttgttatct ccttatattc atggaataaa aactgaccag gttatgagtg gcgataaacc
     1141 cataacaata caacaacatg aaaatgaatg gaacgagata ttaagcaata tcgaaggtgc
     1201 agtagacata ttagcttttc aagatgggca agtggattat catgaacttc cagattatct
     1261 tgaagtgaat aaaaaattag ctgatgcaca taaaatgcgc tgctggacaa acattgaatc
     1321 ttttgatcgt gatatgccaa tacgttttct ccctataaaa tgggaaaaat tattgttaaa
     1381 actggaagca gcacgtaaag ccggaatgga agatgctatt acctttgaat tttctcattt
     1441 tatgagtcct aattcaactt atttgcaagc taaatatcta tacgataggt ataaagaaca
     1501 ctttaatttt taaatttccg attaataagc tattcacttc tattttttac ctaaatacag
     1561 tttttccagt tctttggctt tttccaaaca acggggattt gaagtatcaa ttaaacggct
     1621 atccaatttg ggtacacgaa tgggtgtgcc aacaggaatg ttatctggat gagtaattcg
     1681 atcacgattt gcttcataaa tataaaccca aaaaacaggc acgccatagt aacgtcttga
     1741 aagtccagct aatctgctgc cttccataat tttttcagtt gtaataaatt cactataata
     1801 tcggggtgta tcaaataaaa tttgcaaact atctacctca ttcgctgatt gtttactcac
     1861 ttctccaaga gaatcttttg taacaaatgg ctgctctgtt ataaaatttt tatttccact
     1921 taaaaccaaa ttcttatcag tcttaatttt taataaagta ttaacatatt catttacagg
     1981 agaataaaaa taatataaac ttacacctcc agcaataata atgaacagaa ccaatggtag
     2041 ccaaaaatgt cttctttttc gaggcgaagg aatatctgat agattatctc ctttaaaagg
     2101 tcgcgaagga attcctaatg gttctaaatt ttcattttta tatgactcat caaaattatc
     2161 tttctcgttt ttgctactta taattgaagg tggagaaaca atctcttctt gttcttcgtt
     2221 attataagac actaaaattt cttcattttt ttcttcgatt atttcttcag gaatattttc
     2281 ctcttctttt tcttgttctg aactttcctt tttttctttc atgggaacgt tttcgacagc
     2341 ttcattttgc aaagaagagt aagaaggagg agaaagattt tttatttcct cgagaaggct
     2401 cttgatttca gtggcttgtt cgttgaaaat actaagaggt tccaaaggag gctcttcgct
     2461 gacaaaattt tcgtctttat cttcaacatc aagttctacg ggttcaaggt aagaaaaagg
     2521 cttatttaca agatctcgta aaatttcatc aggtgtaaaa attactttac aacgacgagg
     2581 tataacaaat tctcttctat tttgaaaatt taaaattttg tgaggaccat gtcgtactac
     2641 ctgaaaaatg ccaaaatttt ttattgtcac cgtttctcca tccaacaaag cactttctaa
     2701 cgccgcaaaa aaagctttaa aaaattcttc tgcttctctc ctactcgtct gaacttcttc
     2761 aacaagtaag tccaccaacg attcgatatt taatctagca tcactcattt ttaaaaaatt
     2821 tgtttagctt ttttgctaat gcataacttt gcttaaa
//
LOCUS       DAOTKS010000205         2846 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_28727_length_2846_cov_5.420638, whole genome shotgun sequence.
ACCESSION   DAOTKS010000205 DAOTKS010000000
VERSION     DAOTKS010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28727_length_2846_cov_5.420638"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..1598
                     /locus_tag="P5236_04770"
     CDS             246..1598
                     /locus_tag="P5236_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRU63709.1"
                     /translation="MATQKKNGNLVAIITMIFLFGMISFVTNLAAPIGVIWKNQPQFE
                     GSNFLGMLGNMMNFLAYLFMGIPAGKLLTRIGYKKTALAAIAVGFFGVLIQYLSGIIG
                     VGSQLFALPANFFVYLLGAFVAGFSVCMLNTVVNPMLNLLGGGGNKGNQLIQIGATFN
                     SLAATLTPMFVGALIGDITKERVLSGEITINSVNPILYIALGVFAATFLILLFIPIKD
                     PDENRSRNIEIKYEHSPWNFRHFVLGAIAIFIYVGVEVGIPGTLNFFLADVSDKGAAL
                     NPQKAAAIGGFVAGTYWFLMLIGRFIGSLIGGKISSKTMLTSVATGGIILVLLAIFLP
                     KNLTTSMPVFTGSSFGMVQVPLIALFLVLCGLCTSVMWGCIFNLAVEGLGKYTAAASG
                     IFMMMVVGGGILPLLQNWIADISTYQTSYWVIFVGLLYILFYAVIGSKNVNKNIPVEI
                     "
     gene            1622..2578
                     /locus_tag="P5236_04775"
     CDS             1622..2578
                     /locus_tag="P5236_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRU63710.1"
                     /translation="MEKPYVIGIDMGGTNTVFGIVDVRGNVLSKSAIKTTAHSDVNLY
                     IDDLYNELMKLIVAEGGIEKIRGIGVGAPNGNFYTGNIEFAPNLPWKGIIPFSSLLRE
                     KFGIPVALTNDANAAAIGEMTYGIARGMKDFIMITLGTGVGSGIVIDGKLVYGHDGFA
                     GELGHVTAIRNGRLCGCGKNGCLEAYASATGVARTAREILETTNQESILRNIPLADIT
                     SKDVYDAAKEGDEVAIEIFNFTGKILGELFADFVAFSAPEAIILFGGLSKAGEFILGP
                     IRENMEKNLLPIWKGKIKILFSILKESDAAILGASALAWELQ"
BASE COUNT          892 a          379 c          547 g         1028 t
ORIGIN      
        1 aaagaaacca gaagacattt atatcaacaa atgagcgaat ttttatttat gaatttgtaa
       61 acgaattata aatcaaaaca aaaatattaa ttgagacaaa aaatatgttg taaaacattg
      121 tttatctgat gtattttttg atgctctttt taagaaattt gacccaaaaa tggtactttt
      181 gttgttctat ctttttattt aaactgtaaa tttaacatta ttaacttaat taatttcgaa
      241 acaatatggc tacacagaaa aaaaatggga atctcgtagc aattatcaca atgattttcc
      301 tttttggaat gatttcattt gtgactaatc ttgcagctcc tattggagtt atttggaaaa
      361 atcagccaca atttgaaggc tcgaatttcc tgggtatgtt aggaaatatg atgaactttt
      421 tggcttatct gtttatgggt attccagcag gaaaattgtt aacaagaatc ggttataaaa
      481 aaactgctct tgctgcaatt gctgtaggat tttttggtgt cttaatccaa tatttatctg
      541 gaattattgg agtgggttct cagctttttg ctcttcctgc taatttcttt gtttacttat
      601 tgggtgcctt tgtggcaggt ttttcggtat gtatgttgaa caccgttgtt aatcctatgt
      661 taaatcttct tggtggaggt ggcaacaaag ggaatcaatt aatccaaatt ggagcaacat
      721 tcaattcttt ggctgctaca ctaacaccta tgtttgtagg tgccttaatt ggtgacatta
      781 caaaagaacg tgttttatca ggtgaaatta ctattaatag tgtaaatcct atcctttata
      841 ttgctttagg tgtttttgca gctacttttc tgattctatt attcattcct attaaagatc
      901 cagatgaaaa tagaagtaga aatattgaaa ttaaatatga acatagtccc tggaattttc
      961 gtcactttgt attaggtgct attgctattt ttatttatgt tggagtagaa gtaggaattc
     1021 caggtacact taattttttc cttgcagatg tttctgacaa aggagcagca ttgaatccac
     1081 aaaaagcagc agctattgga ggatttgttg ctggaacata ctggttttta atgcttattg
     1141 gtcgtttcat cggaagtctg attggtggta aaatttccag taaaactatg ttgacaagtg
     1201 tagctacagg agggattatt ttagttttac ttgcgatttt cttacctaaa aatttaacaa
     1261 cttctatgcc tgtatttact ggttcatcat ttggtatggt acaagttcct cttattgctt
     1321 tgtttttagt actatgtggt ttgtgtacct cagttatgtg gggatgtatt tttaatctag
     1381 cagtggaagg gcttggaaaa tatacagcag cagcttcagg aatatttatg atgatggtcg
     1441 ttggaggtgg tattttacca ttattgcaaa attggattgc agatatttct acttatcaaa
     1501 ccagttattg ggtaattttt gttggattgc tatatattct gttttatgcc gtaattggct
     1561 caaaaaatgt aaataaaaat attcccgttg aaatttgaaa aacaaaataa aaaatttaat
     1621 tatggaaaaa ccttatgtaa taggcattga catgggagga acaaataccg ttttcggtat
     1681 tgtagatgtt cggggaaatg tattaagcaa aagtgctata aaaacaactg cacatagtga
     1741 cgtaaatctt tatatagacg acctttataa tgaattaatg aaattaattg tcgctgaagg
     1801 tggcatagaa aaaattagag ggattggagt tggtgctcca aatggaaatt tttatacagg
     1861 taacattgaa tttgctccta atttaccatg gaaaggaatt attccttttt cctcacttct
     1921 tcgtgagaaa tttgggatac cagtggcttt gacaaacgat gcaaatgctg ctgcaatcgg
     1981 agaaatgacg tatggtatag ctcgcggcat gaaagatttt attatgatta ctttaggtac
     2041 aggtgtagga agcggtatcg tcattgatgg aaaattagtt tatggtcacg atggctttgc
     2101 tggagaatta ggacacgtaa ctgccataag aaatggtcgt ttgtgtggat gtgggaaaaa
     2161 tggttgtctt gaggcttacg catcagctac cggagttgct cggacagcaa gggaaatcct
     2221 tgaaacaacc aatcaagaaa gtattttacg gaatattcct ttagctgata ttacttcaaa
     2281 agatgtttac gatgcggcga aagaaggcga cgaagtagct attgaaattt ttaattttac
     2341 gggaaaaatt ttaggagaat tatttgctga ttttgttgct ttcagtgccc ctgaggctat
     2401 tattcttttt ggaggtttat caaaagcagg tgaatttata ttaggtccta ttagggaaaa
     2461 catggaaaaa aatcttcttc ctatctggaa aggaaaaatt aaaattctat tctcaattct
     2521 taaagaatcg gatgcagcca ttttaggagc aagtgcttta gcttgggaac tccaatagaa
     2581 ttcttaattc attgattata aaaaccaaag gttacaattt ttcttattgt catctttggt
     2641 tttttttttg taaattattt ttatcattgt aataaaccat tcattttcat aaaattacac
     2701 agaaaatttt ttataagaaa tttttaattt gctataagat aaaaatgcaa aaagcatttt
     2761 tggattccct ctgttataag taggaatctg ctcttaaatt tgattcttaa gggttgattg
     2821 ataaaaaaga tattcaatta tttgag
//
LOCUS       DAOTKS010000206         2846 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_28730_length_2846_cov_4.149409, whole genome shotgun sequence.
ACCESSION   DAOTKS010000206 DAOTKS010000000
VERSION     DAOTKS010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28730_length_2846_cov_4.149409"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1750)
                     /locus_tag="P5236_04780"
     CDS             complement(<1..1750)
                     /locus_tag="P5236_04780"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRU63711.1"
                     /translation="MRKLVVVLFIVMHSFTILIAQTSLKGVVKIAETDNPLPGVKVTL
                     LKQNISTVTNPNGEFTLSYLEAGDEEIRFTKDGYFSQIKFIHLKADAENDLGVIYLRE
                     DLAQQMKQDIILQLSESQLENEEGRATQNISPVLASKGDVYLSQASYSFSPMRFSFRG
                     YDQNYESTYINGVYFNDIERGLFNYSSLGGLNDAMRNQDKTYGISPATFSYGNLGGVT
                     NIITKASTYATGTKANVAYSNRSYKLRGQATYATGLMQNGWAFAGSAVVRWADEGIIE
                     GTFYNSAGFFFSAEKVFNKQHSLSFVAFGAPTQRAQQGATTQETFDLVGSIYYNPYWG
                     YQEGKKRNSRIVKSFDPAAILSHEFKIDEKKLLKTGIAFHYNFYSNSALAYYNAPDPR
                     PDYYRYMPSFQQNEDVRNQMISLWKTDPNVSQVNWEELYLANYRNNIVNPNGTAKYAV
                     ERRHNNAMEIALNSTYNQQFTKKLKLTGGIEGKMNKGMHYKTMDDLLGGNQWIDIDQF
                     AERDFPADPNIIQNDLRNPNAVIHKDDTFGYHYNINTLYASAFIQNEWTLPQFDIYYA
                     GKLSYTQFYRYGFMQNG"
     gene            2164..>2846
                     /locus_tag="P5236_04785"
     CDS             2164..>2846
                     /locus_tag="P5236_04785"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRU63712.1"
                     /translation="MKKHLLFLLLLIIFLFFGFSSKNSVAFSSDKVNVQVACVAFYNL
                     ENLFDTIDNENVNDEEFLPNGPYRWNSMKYYSKLEHLSYAISQIGLDVTPVGAIIVGV
                     SEVENRGVLEDLVKQPTLINRSYEIVHYDSPDRRGIDVALLYNPRYFIVTNSKSYRLH
                     TQDTTFRTRDQLMVSGYLFDEKIHVIVNHWPSRSGGELASRPKRNAAAALTRSIVDSL
                     YRVDPKAKI"
BASE COUNT          928 a          459 c          460 g          999 t
ORIGIN      
        1 agccattttg catgaaacca taccgataaa attgtgtata acttaattta cctgcataat
       61 aaatatcaaa ttgtggtaat gtccattcgt tttgaataaa ggcacttgca taaagagtat
      121 tgatattgta atgatagcca aaagtgtcat ctttatgaat aacagcattg gggttacgta
      181 aatcattttg aataatgttt ggatcagctg gaaaatcacg ttcagcaaac tgatctatat
      241 caatccattg atttccacct aacaggtcgt ccatcgtttt atagtgcata cctttattca
      301 tttttccttc aataccgcca gtaagtttaa gtttttttgt aaactgttga ttataagtag
      361 aattcaaggc tatttccatt gcattattat gacgccgctc tacagcatat tttgctgtgc
      421 cgttgggatt tacaatgtta tttcggtagt ttgccaaata aagttcttcc caattgactt
      481 gtgaaacatt tggatcggtt ttccacagag agatcatttg atttctaaca tcttcatttt
      541 gttgaaaact aggcatataa cggtaatagt caggacgagg atctggagca ttataataag
      601 caagggcaga attactataa aaattataat ggaaggctat gccggttttt aataattttt
      661 tttcgtcaat tttaaattca tgagaaagaa tagcagccgg atcaaaactt ttcacgattc
      721 ttgaattacg ttttttccct tcttgatatc cccaataagg attatagtaa atggagccta
      781 ccaaatcaaa ggtttcttgt gtagtagcac cttgttgagc tcgttgagta ggagctccaa
      841 aagccacaaa tgataaacta tgttgcttat tgaagacttt ttcggcagaa aagaaaaatc
      901 cagcagaatt ataaaatgtt ccttcaataa ttccttcatc tgcccaacgt actactgctg
      961 agccagcaaa agcccacccg ttttgcatca atcctgttgc ataagtagct tgtcctctaa
     1021 gtttgtaaga acgattgctg taggcaacat ttgcttttgt gccggttgca taagtactgg
     1081 cttttgtaat aatgttggta acgcctccaa gattgccata actaaaagta gctggactaa
     1141 ttccataagt tttatcttga tttcgcatgg catcatttaa gcctcctaaa gaggaataat
     1201 taaacaaacc tctttcgata tcgttaaaat aaacaccatt tatataagtt gactcataat
     1261 tttgatcgta accccggaaa ctaaaacgca tgggactgaa ggaataactt gcctgagaaa
     1321 gatacacatc gcctttactt gccaatacag gtgaaatatt ttgagtagct cgtccttctt
     1381 cattttccaa ttgactttcg cttaattgaa ggataatatc ctgtttcatt tgctgtgcaa
     1441 ggtcttcacg caaataaatg acaccaagat cgttttctgc atccgctttt aaatgaatga
     1501 atttaatttg actgaaataa ccatctttgg taaaacgaat ttcttcatcg cctgcttcta
     1561 aataacttaa agtaaattct ccattagggt tagtaaccgt agaaatgttt tgttttaata
     1621 aagttacttt aacgccaggc aaaggattat cagtttctgc tattttaact actcctttta
     1681 atgaggtttg tgcaattaaa attgtaaaag agtgcataac gataaataaa accactacta
     1741 attttctcat aagtaaaaaa ttaatttatg taaaatttat ttaaaatatt ctgtatgaat
     1801 ttttaaaaag gggtgacaag gtaaaacttt tttttgaatt attgaataaa ttttcagata
     1861 aaattttttc aacaggtttt gtttttctat tttttacaac taaatatggc aagcaattta
     1921 taaaattcta attgaaaacc agaagaaaag ctcatataat gaaaaattat aataaaaatt
     1981 taacatttta ctaaaaatat tttgttgagt gctttaaatc agcgaattgg attaatgata
     2041 aaaaagcaaa aatattttag ggaatgatta aaattgaatt ttcctccgaa aattgttttt
     2101 tctttcaaat attttctaca cttttgttgt tcttttaaga aaataaatta aacattttca
     2161 tatatgaaaa aacatttgtt atttctgctg cttttaatca ttttcctatt ttttggcttt
     2221 tcttctaaaa actcagtagc attttcgtct gataaagtta atgttcaagt tgcatgcgtt
     2281 gctttttata atctggaaaa tttatttgac acgattgaca atgaaaatgt aaatgatgaa
     2341 gaatttttac ccaatggtcc ttatcgttgg aattctatga aatattattc aaaacttgaa
     2401 catctttcgt atgctatttc tcaaattgga ttggatgtga caccagttgg agccattata
     2461 gttggtgtat cagaggttga gaatagaggt gtgttagaag atttggttaa acaaccaact
     2521 ttaataaatc gttcttatga aattgttcat tatgacagtc cagaccgacg tggcattgat
     2581 gtggcgctat tatataaccc cagatatttt attgtaacca atagcaaatc atatcgatta
     2641 catacacaag acaccacttt tcgcacccga gatcaactaa tggtaagtgg ttatttgttt
     2701 gatgaaaaaa ttcatgtgat tgtcaatcac tggccttctc gttcaggagg tgaattagct
     2761 tcacgaccta aacgaaatgc tgcggcagct cttacccgtt ctattgttga ttctctatat
     2821 agagtggatc caaaagcaaa aattat
//
LOCUS       DAOTKS010000207         2830 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_28922_length_2830_cov_5.411171, whole genome shotgun sequence.
ACCESSION   DAOTKS010000207 DAOTKS010000000
VERSION     DAOTKS010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28922_length_2830_cov_5.411171"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="P5236_04790"
     CDS             complement(<1..858)
                     /locus_tag="P5236_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRU63713.1"
                     /translation="MSTIKGKVWQVIGPVVDVFFPLDEKNEYKLPPINNALSISLPDE
                     KNLILEVQQHIGENIVRTIAMDSTDGLRRGLEVIDLGRSISMPIGNQVKGRLLNVIGE
                     PIDGLIPLSKNNVSPIHCEPPKFEELTTTDEILYTGIKVIDLLAPYVKGGKIGLFGGA
                     GVGKTVIIMELINNIAKGHKGYSVFAGVGERTREGNDLLREMVEAGVIHYGEEFNKAM
                     KDGKWDLSKVDYEQLKNSQATLVFGQMNEPPGARFSVALSGLTVAESFRDSEESENRD
                     ILLFIDNIFR"
     gene            complement(993..2780)
                     /gene="lepA"
                     /locus_tag="P5236_04795"
     CDS             complement(993..2780)
                     /gene="lepA"
                     /locus_tag="P5236_04795"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRU63714.1"
                     /translation="MKTIRNFCIIAHIDHGKSTLADRLLEYTHTVVGKELHDQVLDNM
                     ELERERGITIKSHAIQMNYQYGGEEYILNLIDTPGHVDFSYEVSRSIAASEGALLVVD
                     ATQGIQAQTISNLYMALEHNLTIIPVINKIDMDSARPDEVEDEIVELLGCSREEIIRA
                     SAKTGEGVKEILKAIVTRIPPPQGNPEAPLQCLIFDSVFNPFRGIIAYFKIVNGTINK
                     GDWVKFVSTGKEYFADEIGVLKLNFLPMESLSAGNVGYIISGIKTSREVKVGDTITHV
                     KRSCQEAIAGFEEVKPMVFAGVYPIDTEDFEDLRSSIEKLQLNDASLTFEPETSIALG
                     SGFRCGFLGMLHMEIVQERLDREFNINVITTVPNVSYWVYTKKGEKIEVNNPASMPDP
                     ALIDHIEEPYIIASIITPTEFIGQIMTLCLGKRGELIKQNYVSGNRIELIYDMPLGEI
                     VFDFYDKLKSISRGYASFDYHMNGYRPSRLVKLDILLNGEPVDALSSLIHADNAMTLG
                     RRMCEKLKELIPRQQFEIAIQAAIGSKIIARETIKPVRKDVTAKCYGGDITRKRKLLE
                     KQKKGKKKMKQIGSVEIPQKAFLAVLKLD"
BASE COUNT          895 a          597 c          424 g          914 t
ORIGIN      
        1 gcggaaaata ttgtcgataa acaatagaat atctcgattt tcactttctt cgctatcgcg
       61 gaacgattct gccacggtca gtcccgataa tgccacagaa aaacgtgctc caggcggttc
      121 attcatctgt ccgaaaacta acgtagcttg tgaatttttc aattgctcgt agtcaacttt
      181 tgataaatcc catttcccat ctttcatggc tttattgaat tcttcgccat aatggataac
      241 acctgcttcg accatttcac gcaacaagtc atttccttca cgggtacgtt caccaacacc
      301 agcaaaaaca gaatatcctt tatgtccttt ggctatgtta ttaataagtt ccattataat
      361 aacagtcttg ccaacaccag ccccaccaaa aagaccaatt tttccacctt ttacataagg
      421 tgccagcaag tcgataactt taatgcctgt atataaaatt tcatcagtcg ttgtaagttc
      481 ttcaaattta ggaggctcac aatgaatagg agaaacatta tttttactca aaggaattaa
      541 accatcaatg ggttcaccaa ttacatttaa taatcgtcct tttacttgat ttccaatagg
      601 catagaaata gatcttccca gatcaattac ttccaatcca cggcgtaaac catcagtaga
      661 atccatcgct atggttcgaa caatattctc accgatatgt tgctgtactt ctaatatgag
      721 atttttttca tcaggaagtg aaatgcttaa agcattatta ataggaggta atttatactc
      781 atttttctca tcgagaggga aaaaaacgtc cactaccgga cctataactt gccacacttt
      841 cccctttatt gttgacattt tttacaaaaa atttaatttt tattctcatt ctcaaaaatt
      901 gtggcaaatg tagccaaaac tttgttccta agtaaaaaag aatgtttaaa tatgctcaaa
      961 attattcatt ttaatgttaa aaattttgtc cctcaatcta actttaaaac agccaaaaat
     1021 gctttttgtg gaatttctac cgaaccaatt tgcttcattt ttttcttacc ttttttctgc
     1081 ttttctaata atttcctttt acgagtaata tcgcctccgt aacatttggc tgtaacgtct
     1141 tttctgactg gttttatggt ctctcttgca ataatttttg aaccaatagc tgcttgaata
     1201 gcaatttcaa actgttgtcg cggaatcagt tcttttaatt tttcgcacat ccgtcgtccc
     1261 aatgtcatag cattatctgc atgaatcaaa gaagaaagag catcgaccgg ctcaccattg
     1321 agaagaatat ctaactttac caaccgggaa ggacgataac cattcatgtg ataatcgaaa
     1381 gaagcatacc ctcttgaaat acttttgagc ttatcataaa aatcaaaaac aatttcacct
     1441 aatggcatgt cataaatcag ttcaattcta tttccagaaa cataattctg tttgattaat
     1501 tcgccgcgtt tccctaaaca aagggtcatt atctgtccga taaactcagt aggcgtaatg
     1561 atggaagcta tgatgtatgg ttcttcaata tgatcaatca aagcaggatc gggcatactc
     1621 gcaggattat ttacttctat cttttctcct tttttggtat aaacccagta agaaacattt
     1681 ggtacagtag taatgacatt tatattaaac tctctatcga gacgttcctg aacaatttcc
     1741 atgtgcaaca tacccaaaaa accacaccta aagccagagc ctaatgctat ggaagtttca
     1801 ggttcaaatg tcagtgaagc atcgttcaat tgcaattttt ctattgaaga gcgtaaatct
     1861 tcaaagtctt ctgtatcaat tggataaaca ccagcaaaaa ccataggctt tacttcttca
     1921 aaacctgcta ttgcttcctg acaagaacgc tttacatggg taatggtatc acccaccttt
     1981 acttctcttg aagttttaat tcccgaaata atataaccca cattacctgc acttaaactt
     2041 tccattggta gaaaattaag tttcaaaaca ccaatttcat cagcaaaata ttctttcccg
     2101 gtcgaaacga atttaaccca atcgcctttg ttgattgttc catttacaat tttaaaataa
     2161 gcaataatcc cgcgaaaagg attaaaaaca gaatcgaaaa tcaaacattg aagcggagct
     2221 tcaggattgc cttgcggagg cggaattcga gtaacaatag cttttaaaat ttctttcaca
     2281 ccttcgccgg tttttgcact ggcacgaata atttcttcac ggctgcaacc taacaattcc
     2341 actatttcat cttcaacttc atcaggtctg gcactgtcca tatctatctt gttaatcaca
     2401 ggaataatag ttaaattatg ctccagagcc atatagagat ttgaaatggt ttgtgcctga
     2461 ataccctgtg tagcatctac cacgagtaaa gctccttcgc ttgcagcaat ggaacgtgat
     2521 acttcatagg aaaaatctac atgccctggc gtatcaatca gatttaaaat atattcttct
     2581 ccaccgtatt gataattcat ttgaatggca tgacttttta tagtgattcc ccgttctcgt
     2641 tccagctcca tattatcaag cacttgatca tgaagttctt tgccaacaac agtatgagta
     2701 tattccagca aacgatcggc taacgtgctc ttaccgtggt caatatgagc tatgatacaa
     2761 aaatttcgga tagtcttcat tagtttttat tttaaacaca aaattacaaa aaaacaagga
     2821 aataggaaag
//
LOCUS       DAOTKS010000208         2827 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_28955_length_2827_cov_4.369769, whole genome shotgun sequence.
ACCESSION   DAOTKS010000208 DAOTKS010000000
VERSION     DAOTKS010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28955_length_2827_cov_4.369769"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(232..2613)
                     /locus_tag="P5236_04800"
     CDS             complement(232..2613)
                     /locus_tag="P5236_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRU63715.1"
                     /translation="MSRIKLRTWLYYVLMLLLFCVGAYLLIQKGSDVEANYHVAKQNV
                     SPQVVSPATYLSDWEQFKISVSHNIGEPITHLLLQIIAILLVSRLLTFLFAKINQLSV
                     IAEILAGIVLGPSLLGHFFPQVFNFLFAANSLPNLYILSQIGLILFMFVIGMELDMKA
                     LKEKASQVLVISHASILIPYFLGMLLAYIYFEEFAVNQTKFLPFALFIGISMSITAFP
                     VLARIVQEKGLTKTHLGTISIASAAIDDVTAWCLLALVIAISKTGDFVSALYTVGLAV
                     AYILFMFFVIKPFLKKIGEIYQNIETLNKSIVAFLFLILISSSFITQFIGIHALFGAF
                     LAGVVMPPIPNFRKLVIEKVEDVSLTLLLPLFFVFTGLRTEIGLLNSPYLWGICGIFI
                     ATAIVGKFVGGAFSARFLGESWHDSLALGTLMNTRGLMELIVLNIGYEMHIIPPSIFV
                     MLVIMAIVTTFMTTPMLSLIEKVFPAKNVEEEYIRKQSMGIFKVLIAVGNPENGKMLL
                     RVAKNVLDGVKNTLSVTVLHITPGTDTNPIYGDQFAEESFNKVKQEAEQLNIPIETEY
                     KVVDSIENAIVRMANTNNFDFLLVGAGLSLSGIPFFKERSVFKNIGWLNRLINNISKR
                     QVVFYPGTLIKDKTKYFIEQSNCTVGVFVNRGFSEVSSAVVLLYNEEDEFLLHYARRL
                     LRNNSNAFVTIFDLNKVVASSEKVARYIEDLEKQFPSSVKLNKQNKINSNTFSKFSLL
                     FISYQTWNALLETDRTYLSDIPSTLIINKKENRFHSDGRRRQGLHQTSEDNAL"
BASE COUNT          959 a          556 c          499 g          813 t
ORIGIN      
        1 tatctctttt ttctccttta ttttcagccc tttctgaagt gcgaaacttt agttaggtat
       61 aaaacttttg ccattggtaa gtattttgaa aaaattaaca gccaattggc gctcgaaatt
      121 gctttataaa aaagaggcga acatggtttc gcaaactaag gaatagattt tattgtttaa
      181 ctttctttaa attgtatcat gtgggtaacc taagttgcca tttcaacggg actataatgc
      241 gttatcttcg gaagtttgat gtaatccttg tcggcgtctg ccatcagaat ggaagcggtt
      301 ttcttttttg ttgataataa gcgtagaagg tatatccgat agatatgttc tgtcagtttc
      361 caacaatgcg ttccatgtct gataactgat gaacagtagg ctgaatttgg agaaagtgtt
      421 ggaattgatt ttattttgtt tgttcagctt aacggaagaa ggaaattgtt tttccaaatc
      481 ttcaatgtac cgggcaactt tctcgctgga agctactact ttgttcagat cgaaaatggt
      541 aacaaacgca ttgctgttgt tgcgcaaaag acgacgcgca taatgtaaca aaaattcgtc
      601 ttcctcattg taaagcaaga caacagcaga actaacttcc gaaaatccac gatttacaaa
      661 cacgcccact gtacaattgc tttgttcgat gaaatatttg gttttgtcct taattaaggt
      721 tcccggataa aacaccacct gcctttttga aatattgttg atcagtctat tcaaccatcc
      781 gatattttta aatacagatc tctccttgaa gaaagggatt cccgacaagg aaagtccagc
      841 tcccaccaaa agaaaatcga aattgttggt atttgccatt cggacaatag cgttttctat
      901 actgtccacc actttatatt ccgtttcaat gggaatattg agctgctcgg cttcctgttt
      961 aaccttattg aaactttcct cagcaaactg atcgccataa ataggattgg tgtcggttcc
     1021 gggcgtaata tgcagcaccg taactgagag agtatttttc actccatcca acacgttttt
     1081 ggcgaccctt aataacattt tcccattttc aggattgccc acagcaatta acaccttgaa
     1141 aatacccatc gactgtttgc gaatatattc ttcttccaca ttttttgccg gaaaaacttt
     1201 ctctatcaga ctcaacattg gagtcgtcat aaatgtggta actatagcca taatcaccag
     1261 cattacaaag atggaaggag gaataatatg catttcgtaa ccgatattta acacaatcaa
     1321 ttccatcaac cctcgtgtat tcataagtgt acccaatgca aggctgtcgt gccacgactc
     1381 gccaaggaaa cgtgccgaaa atgctccacc cacaaatttt ccgacaatgg ctgtggctat
     1441 aaagattcca caaattcccc ataaataagg agaattgagc aaccctattt cggtacgtaa
     1501 gccggtgaat acaaaaaaca gaggcaataa caaagtcaat gaaacatctt caactttttc
     1561 gatcaccagc tttctgaaat taggaatagg aggcattacg actccggcca gaaaagcacc
     1621 aaacaaagcg tgaataccaa taaactgtgt aataaaggaa gaagaaatca ggatcagaaa
     1681 aagaaatgca acaatacttt tgttgagggt ttcgatattt tggtaaattt caccgatttt
     1741 tttcagaaaa ggttttatta cgaagaacat aaacaaaata tacgcaactg ccaatccaac
     1801 cgtataaagt gcacttacaa aatcaccggt tttggagatg gcgatgacca gcgccagcaa
     1861 acaccaagct gtaacatcat caatagcagc actggctatt gaaattgtcc ccaaatgtgt
     1921 cttggttaat cctttttcct gaacaattct tgctaaaacg gggaaagcgg tgatactcat
     1981 tgaaatccct atgaataaag caaaaggaag aaatttagtt tgattgacag caaactcttc
     2041 aaaataaata tatgccagca gcattcccaa aaaataagga atgagaatac tggcatgact
     2101 gataaccagc acctgacttg ctttttcttt cagcgccttc atatccagtt ccataccaat
     2161 gacaaacatg aaaagaatca acccaatttg gcttaaaata tacaagttag gcaaagaatt
     2221 ggcagcaaac agaaaattga aaacttgagg gaaaaaatga cccagcagag aaggtcccaa
     2281 tacaataccg gctaaaattt ctgcaattac cgagagttga ttaattttag caaaaagaaa
     2341 cgttagaagt ctcgaaacca aaagtatggc aatgatttgg agcaataaat gggtgatggg
     2401 ttcaccaata ttatgagaca cagaaatttt aaactgttcc caatcgctga gataagtagc
     2461 cggcgaaaca acttgagggg aaacgttttg ttttgccaca tgataatttg cctccacatc
     2521 actccctttt tgtatcaaca aataagctcc tacacaaaaa agaagcaaca tgagcacata
     2581 ataaagccag gttcgcaatt tgattctgga catagaattt actttatcat gtcgtactaa
     2641 aaagaaaaat tattcttaaa atcctctaca ggaaagcaaa aggcgtaaag aaactttctt
     2701 tttcaaaagc aaaaatactg aaaattttta aaaaactcgg ctcagagcca aaattttctt
     2761 gatttattaa tttttgagtc cacttgaata agtcttattt atcaaaaatg agcaaaaaac
     2821 atacttg
//
LOCUS       DAOTKS010000209         2826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_28972_length_2826_cov_5.079394, whole genome shotgun sequence.
ACCESSION   DAOTKS010000209 DAOTKS010000000
VERSION     DAOTKS010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28972_length_2826_cov_5.079394"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1229
                     /locus_tag="P5236_04805"
     CDS             <1..1229
                     /locus_tag="P5236_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HRU63716.1"
                     /translation="FGTLYNTYSFFALYANVDHFTYAEEEIPVEKRPEIDRWIISLLN
                     TLIKEVTNDYDDYDPTRAGRAIADFVCENLSNWYVRLNRKRYWGGEYDEDKISAYQTL
                     YTCLEIVSQLMAPIAPFYADQLFIDLNAVTEKHKEASVHLTDFPKYNENLIDKNLEEC
                     MQLAQQVSSMILSLRKKANKRVRQPLAKAIVPAPDEKTYQQLKYVADLIKTEVNVKEL
                     EIIPQNGKMHNLIKKVKPNFKLLGKKYGKLMKEITEALANLGNTEIAEIEQKGEYVLQ
                     LSAGEVPISLEEVEIITEDMPGWLTANDGKLTVALDITVTDELLKEGIAREFVNRIQN
                     IRKANDFEITDRITIEIEKRDEINEAINEFSNYIASQTLATSITLVDKLDNTTELDFD
                     DYFVKIKIKKTKEFIQ"
     gene            1333..1710
                     /locus_tag="P5236_04810"
     CDS             1333..1710
                     /locus_tag="P5236_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HRU63717.1"
                     /translation="MAEKTRYSDEELEEFRQIILEKLEKAQKDYEILRNAITNEASND
                     VSDTSPTFKVLEEGATTLSKEEAGRLAQRQLEFIRHLQAALVRIENKTYGICRETGKL
                     IPKERLRAVPHATLSIEAKNKKK"
     gene            1772..2383
                     /locus_tag="P5236_04815"
     CDS             1772..2383
                     /locus_tag="P5236_04815"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HRU63718.1"
                     /translation="MKMSIAKKSALLIFLVLFIDQASKIYVKTHFALGDHVDIFNWFQ
                     IYFIENSGMAFGMQFIGKLFLTFFRIAAVVLIFFYLRSLIKKQFSTGYILTISLLLAG
                     AAGNIFDSVFYGVLFSDSYGKVATFLPPEGGYAPLFYGKVVDMLYFPLIKNSAGEVIF
                     FKPIFNVADSAITIAVFIILFFYRKELNQSFEKKPKQNVSPEK"
     gene            2475..>2826
                     /locus_tag="P5236_04820"
     CDS             2475..>2826
                     /locus_tag="P5236_04820"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HRU63719.1"
                     /translation="MVKVLTEFHLLDGVLYAKDSLYIGNYNTYYYQYVLKKHGVTQAQ
                     FDSSLAWYGKNPREFEKVYTDVILRLNHLQEDIKKEKYHPNYLETTKTNLWYLPTHYL
                     LTKDSIRRNLLFKIQ"
BASE COUNT          983 a          445 c          501 g          897 t
ORIGIN      
        1 tctttggaac gctttacaat acttattcct tttttgctct ttacgccaat gtggatcatt
       61 ttacttatgc cgaagaagaa attcctgtgg aaaaacgtcc tgaaattgat cgatggataa
      121 tttcccttct taatactttg atcaaagaag ttaccaatga ttatgacgat tatgatccta
      181 cgcgagctgg acgcgctatt gcagattttg tgtgcgaaaa tctaagcaat tggtatgttc
      241 gtttgaacag gaaacgttat tggggtggtg aatatgatga ggataaaatt tctgcctatc
      301 agacacttta tacctgctta gaaattgttt ctcaactaat ggcacccatt gcaccttttt
      361 atgctgacca attgttcatc gatttgaatg cagttacgga aaaacataaa gaagcatctg
      421 ttcatttgac tgatttcccc aaatacaatg aaaatcttat tgacaaaaat ctggaggaat
      481 gtatgcaact ggctcaacaa gtttcttcaa tgattctttc gttacgaaaa aaagcaaata
      541 aaagagtgcg tcaaccatta gcaaaagcca ttgttccagc ccccgatgaa aaaacttatc
      601 agcaattgaa atatgtagcg gacttgatta aaacagaagt taatgtgaag gaactggaaa
      661 tcatcccgca aaatggtaaa atgcataatt taataaagaa agtaaagcca aatttcaaat
      721 tgcttggtaa aaaatatggc aaattaatga aagaaattac ggaagctctg gctaatttag
      781 gaaatacaga aattgcagaa atagaacaaa aaggcgaata tgttttacaa ctttcagcgg
      841 gagaagttcc tatttctctg gaagaagtag aaattattac agaagatatg ccaggctggt
      901 taactgctaa cgatggcaaa ctgacagtag cactcgacat aacggtgacg gatgaactgc
      961 tgaaagaagg tattgctcgt gaatttgtca atagaattca aaatattcga aaagctaatg
     1021 attttgaaat tacagataga attaccattg aaatagaaaa gcgggatgaa attaatgaag
     1081 caataaacga attttcaaat tatattgcca gccagacatt ggcaacctct attacgttag
     1141 tggataaact tgataatacc actgaattag attttgatga ttactttgta aagattaaaa
     1201 taaaaaaaac aaaggaattc attcaatgag tttttaaaat tattgctaaa gagaaaaaaa
     1261 atgattattt tcgcagatta tttcaaaggc attaaccaat ccgaaaggat tacttttatc
     1321 attcaatgaa cgatggcaga aaaaacaaga tattcagatg aggagttgga agaatttcgc
     1381 caaattattc ttgaaaagct tgaaaaggct cagaaagatt acgaaatact tcgtaatgcg
     1441 ataactaatg aagccagtaa tgatgtttca gatacttcgc caacatttaa agtgttagaa
     1501 gagggagcta ctaccctttc aaaagaagaa gcaggaagat tggctcaacg gcaattagaa
     1561 ttcattcggc atttacaggc agcattggta cgtattgaaa ataaaactta cggaatttgt
     1621 cgtgaaacag gaaaattgat acctaaagag cgattgcgtg ccgttcctca tgctacttta
     1681 agtattgaag caaaaaataa gaaaaaataa atcttcaagc aaaaagtcgg cagcaaaaaa
     1741 cttgctgccg aactttcata atgacctcca gatgaaaatg tctattgcta aaaaatctgc
     1801 cctgttgatt tttcttgttc tttttattga tcaggcttca aaaatttatg taaaaactca
     1861 ttttgctctg ggtgaccatg tggatatatt caactggttt caaatttatt ttattgaaaa
     1921 cagcggaatg gctttcggaa tgcaattcat cggtaaactt tttcttacct tttttcgtat
     1981 tgcagcagtt gtgttaatat ttttttatct tcgttcctta ataaaaaaac aattttccac
     2041 tggatatatt ttaactattt cgcttttgct ggcaggtgct gcaggaaata tctttgactc
     2101 tgtattttat ggtgttcttt tcagtgacag ttatggtaaa gtggctactt ttttgcctcc
     2161 tgaaggtggt tatgctccct tgttttatgg taaagtggta gacatgcttt attttcctct
     2221 gattaaaaat agtgctgggg aagtaatttt tttcaaacca atttttaatg tggcagattc
     2281 tgctataacg atcgctgttt tcatcattct gtttttctac cgaaaggaat tgaatcaaag
     2341 ttttgagaaa aaaccaaaac aaaatgtttc accagagaaa taaattcttt cgatttttct
     2401 ttttaataag tattattcta tttctaatag ccgcgtgcaa tcgacgtcct aaagatgtac
     2461 tgagtaaaaa ggaaatggtt aaagttctta ctgagtttca cctcctcgat ggagtacttt
     2521 atgcaaaaga cagcctttac attggtaatt ataataccta ttattatcaa tatgttttaa
     2581 aaaaacatgg tgttactcag gctcaatttg attcgtctct tgcatggtat ggaaaaaatc
     2641 ccagagaatt tgaaaaagtt tatactgacg tgattcttcg gctaaatcat ttacaagaag
     2701 atattaaaaa agaaaaatat catcccaatt atcttgaaac tactaaaaca aatctctggt
     2761 atttgcctac acattatctt cttacgaaag attctatcag aagaaatttg ctatttaaaa
     2821 tccagc
//
LOCUS       DAOTKS010000210         2823 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_29018_length_2823_cov_5.013728, whole genome shotgun sequence.
ACCESSION   DAOTKS010000210 DAOTKS010000000
VERSION     DAOTKS010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29018_length_2823_cov_5.013728"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(297..743)
                     /gene="tsaE"
                     /locus_tag="P5236_04825"
     CDS             complement(297..743)
                     /gene="tsaE"
                     /locus_tag="P5236_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005657621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRU63720.1"
                     /translation="MKQPTTLKIPSLSSIHEVAKQFINQMGDDKIFAFYGKLGAGKTT
                     LIKGICKELGVKEMVNSPTFSMINEYETKEEEIIYHFDCYRINKIQEALDIGAEDYFY
                     SGKLCFIEWPENIEQILPDSTIKVYIEEKENQEREVKIIKPSKDKI"
     gene            complement(821..2638)
                     /gene="uvrC"
                     /locus_tag="P5236_04830"
     CDS             complement(821..2638)
                     /gene="uvrC"
                     /locus_tag="P5236_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRU63721.1"
                     /translation="MAQQDLLLQQLDMLPEKPGVYQFYNNKEEIIYVGKAKNLKKRVS
                     SYFNKTHESPRTNVLVKNIASLKYIVVDTEEDALLLENNLIKQYQPRYNVQLKDDKTY
                     PSICITNEEFPRVFKTRNIIQNGSQYFGPYSSVSALNSLLDTIRELYPIRTCKLPLTR
                     ENIKAGKFKVCLQYHIHKCNGPCEGKETVEEYQKNIQQIRQIIQGDANEISKMLWNEM
                     QQLAAEYKFEEAYTIKQKYDLIENYKAKSIIANTIVENTDVFAYDETENVAYITILRV
                     SKGAVIQGYTIEYQKKLEEDKADLLSTAIIELRQKLNSNSKEIVVPFEINYKPKDTQV
                     IIPQRGDRKKLLDLAIQNVQQYKLEKLKQAEKLNPEQRQIQLLKNLQEKLHLDKIPMV
                     IECFDNSNIQGSNAVAACVVYKKGKPSKNDYRKYDIKTVEGPDDYSSMKEVVRRRYTR
                     MLNEEKPLPDLIIADGGIGQMNVIREVLEDELQIHIPIVGLVKDNKHNTRGLLYSFPP
                     KEVGLNPNDSLFRFMAAMQEEVHRFAITFHRNKRSKKQIASELDEIKGIGEKTKNELL
                     RHFKSVKRIKNASKDELSKIIGKSRASIIYNHFHTNLSL"
     gene            complement(2674..>2823)
                     /locus_tag="P5236_04835"
     CDS             complement(2674..>2823)
                     /locus_tag="P5236_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008657907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63722.1"
                     /translation="RFDYDSILSLSTEARQKLMRVDPETIGQASRIPGISPNDINVLL
                     VLMGR"
BASE COUNT          813 a          477 c          424 g         1109 t
ORIGIN      
        1 ctcacgctga aacacatttt ataaaatcca aacttggcga acaatatcaa ttattcgaag
       61 aaataaccaa tttgaataaa gtcaatggaa ttcacgctct tttacctttt aaattagttg
      121 ttcagtagtt ttttaatacg taaatgctat caagaagagg caaatacagt tctggtttgc
      181 ctcttcttat atgtgttaga cgtgtagcac cataatgttt gacatatttc taattaataa
      241 gattcaaaaa gagagactta attttaaata ataagttctc aatcaattaa ttaggctcaa
      301 attttatctt ttgagggttt aattattttc acttctcttt cctgattttc tttttcttca
      361 atataaactt ttatagtaga atctggtaaa atctgctcaa tattttctgg ccattcaatg
      421 aaacaaagtt ttccactgta aaaataatcc tctgctccaa tatccaaagc ttcctgtatt
      481 ttattgatac gatagcagtc aaaatgataa attatttctt cttcctttgt ttcgtattcg
      541 tttatcatag aaaatgtagg actgttgacc atttctttta ctcccaattc tttacaaata
      601 cctttaatca aagttgtttt tccagctcct aattttccgt agaaagcaaa tattttatca
      661 tcgcccattt ggttgataaa ttgtttggct acctcatgaa tagaagaaag cgaaggaatt
      721 tttaaagtag ttggctgttt cattattttg atttttgtta atctttacga gatgctcgta
      781 tttacgtata acttatcaat ttttttgaaa tatcttattc tcacaagctc aaattggtat
      841 gaaaatgatt ataaatgatt gatgctctgc tttttcctat aattttagag agttcatcct
      901 tcgatgcatt tttaatgcgt tttacacttt tgaaatgtct taaaagttca ttttttgttt
      961 tttctccaat tcctttaatt tcgtccagtt ctgaagcaat ttgtttttta cttcgtttgt
     1021 tgcgatgaaa agttatggca aaccgatgta cttcttcctg cattgctgcc ataaatctga
     1081 acaaggaatc gtttggattt aatcctactt cttttggagg aaaactataa agcaagccac
     1141 gcgtgttatg tttattgtcc tttaccagtc caacaatcgg aatatgaatt tgtagttcat
     1201 cctccagtac ttctctgata acattcattt gcccaatacc gccatcagca atgattaaat
     1261 caggtaaagg cttttcttcg tttagcattc tggtatatct tcgtcttacc acttctttca
     1321 tggaagaata atcgtcagga ccttcaacag ttttaatgtc atatttcctg taatcatttt
     1381 tcgaaggttt gcctttttta taaaccacac atgctgcgac agcatttgac ccttgaatat
     1441 ttgaattgtc aaagcattca atcaccattg gaattttatc caaatgcaat ttttcctgca
     1501 aatttttcaa cagttggatt tgtctttgtt ctggattcaa tttttcagct tgcttgagtt
     1561 tttccagctt gtattgttga acattttgta tagcaagatc aagtaatttt tttctgtctc
     1621 ctcgctgagg aataatcact tgtgtatctt ttggtttgta attaatttca aaagggacaa
     1681 caatttcttt tgaattgcta tttaattttt gtcgaagttc tatgatggct gtcgagagca
     1741 aatcggcttt atcttcttcc agcttttttt gatattctat tgtatatcct tgaatgacag
     1801 caccttttga aactcttaaa atagtaatgt aagcaacatt ttcagtttca tcataagcaa
     1861 aaacatctgt attttcaact atcgtgttgg caataattga ttttgcttta tagttttcaa
     1921 tcaagtcata tttctgtttt attgtataag cttcttcaaa tttatattca gcagcaagtt
     1981 gttgcatttc gttccagagc attttactga tttcgttcgc atcgccttga ataatctgac
     2041 gaatttgttg gatatttttt tgatattctt caacggtttc cttcccttcg cacggaccgt
     2101 tgcatttgtg aatgtgatat tgcaagcaaa ctttaaattt acctgctttt atattttctc
     2161 tggtcaatgg aagtttgcaa gttcgaatgg gataaagttc acgaatggta tcaagtaatg
     2221 aatttaaagc acttaccgaa ctataaggac caaaatattg agaaccattt tgaataatat
     2281 ttctggtttt gaaaaccctt ggaaattctt cattggttat acagatgctt ggataggttt
     2341 tgtcgtcttt taattgaaca ttgtatcgtg gttgatattg tttgattaaa ttgttttcga
     2401 gcagcaacgc atcttcttct gtatctacta caatatattt tagggaagca atatttttaa
     2461 ctaaaacatt cgttcgtggc gattcatgcg ttttattaaa gtaagatgaa actcttttct
     2521 taagattttt tgctttgcca acataaatta tttcctcttt attattataa aattgataca
     2581 ctcctggctt ttcaggaagc atatccagtt gttgtaacaa taaatcttgt tgagccattt
     2641 tgcaaaataa tgtttcacgt ggaacattta aaattatctt cccattaaaa ccaataacac
     2701 gttaatgtca tttggagaaa ttcctggaat tcggcttgct tgcccaatag tttcaggatc
     2761 gactcgcatc aatttctgcc gagcttcggt tgataaagat aagatagaat cataatcaaa
     2821 acg
//
LOCUS       DAOTKS010000211         2823 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_29020_length_2823_cov_3.928468, whole genome shotgun sequence.
ACCESSION   DAOTKS010000211 DAOTKS010000000
VERSION     DAOTKS010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29020_length_2823_cov_3.928468"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="P5236_04840"
     CDS             <1..346
                     /locus_tag="P5236_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63723.1"
                     /translation="AVLSGNAYETVENSKITEISHHQGKKQNSIPTQQYWFSITNFPL
                     NIPANEEVRILPTSSFTFCRVLERLHFFKVRYSTLLVKEQYFFLHKVSDYFFSYQALW
                     GYYLFQLRKLLI"
     gene            489..1019
                     /locus_tag="P5236_04845"
     CDS             489..1019
                     /locus_tag="P5236_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63724.1"
                     /translation="MDTNLTIEESFDSLYKNEIGKSKQSPLVGTLLIVVGLILVLVSV
                     SIKSLEGTSMLYLLIMIGSIFAIVGIFVLLFRKTYYISVQTRKKLKPYEIYFDPKERD
                     KLVRIMQNKNLNELKSLKLAVQDGLKLRVFTTADGEACFSQVVTFIPYEYVNVTPVMK
                     HTPEEAKILIETFKLK"
     gene            1475..1939
                     /gene="smpB"
                     /locus_tag="P5236_04850"
     CDS             1475..1939
                     /gene="smpB"
                     /locus_tag="P5236_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HRU63725.1"
                     /translation="MHRPSNIIIKNKRANFEYEILDRYIAGIQLFGTEIKSIRDGKAS
                     LTDTYCIFINHELWVKNMHIAAYSFGTYNNHEVRRDRKLLLTKRELLKLERANKESGT
                     TIVPLKLFINEKGLAKLEIALVRGKKLYDKRQSLKEKEDQRNIERALKQKEH"
     gene            2067..2702
                     /locus_tag="P5236_04855"
     CDS             2067..2702
                     /locus_tag="P5236_04855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRU63726.1"
                     /translation="MTDSEIWLSFKKGEEWAVSYIYKEYSPKLYQYGVKFTVNVPLVE
                     DTIQDLFTELIKNHKTIGETNNILFYLLKSFRRKLFRKMESEKKYSNGTEPEEYQFEV
                     VWSIEHDLIIEEEANQKKTSLLKALDKLTPRQKEVIYLRFTKELDYCSIAEIMNISIE
                     SCRNLISKAIFNIKKGKREKIKFAINIFYLSPNGRNISEVVFDAILLSVIM"
BASE COUNT         1008 a          397 c          409 g         1009 t
ORIGIN      
        1 tgctgtgctt tcaggcaatg cttatgaaac cgttgaaaac tcaaaaatta ctgaaataag
       61 ccatcatcag ggaaaaaagc aaaattctat tccaactcaa caatattggt tttccatcac
      121 taatttccct ttaaatatcc ctgctaatga agaagttaga attttaccta cttcttcatt
      181 tacgttttgc agagttttag aaagattgca tttcttcaaa gttcgttact cgactttatt
      241 agtgaaggag caatattttt ttcttcataa agtttcagat tatttttttt cttatcaagc
      301 actatgggga tattatcttt tccaacttcg taaattacta atttgatttt tccatcttta
      361 tttaattctt tcttaaacat ttaatcaatt agatttctct aattttttta aattattttt
      421 attttaattt ctctaaaaat caattaataa gtatctgaaa aatacttact taaaagaatt
      481 atataatcat ggatacaaat ttaacaattg aagaaagttt tgattctctt tataaaaatg
      541 agatcggaaa atctaagcaa tcacctttgg ttggaaccct tttaatagtt gtagggttaa
      601 ttttggtatt agtaagtgtt tctatcaaaa gtttggaagg tacttccatg ctttatttat
      661 tgataatgat aggtagtatt tttgctattg tgggaatttt tgttctacta tttcgaaaaa
      721 cttattatat ttccgttcaa acacgtaaaa aattaaaacc ttatgaaatt tattttgatc
      781 cgaaagaaag agataaatta gtgagaatca tgcaaaataa aaatttgaat gaacttaaaa
      841 gtttgaagtt agctgtccaa gatggactta aacttagggt attcactact gctgatggag
      901 aagcatgttt ttctcaagta gttactttta ttccttatga atatgtaaat gtaactccag
      961 ttatgaagca tacacccgaa gaagcaaaaa tcctaataga aacatttaag ttgaaataga
     1021 aatttatatt aattttttat tttttaaaaa agggttgacc aaataatcaa cccttttttc
     1081 aacagttata ttttatggct gatttcccct cattctttaa attcagataa ccattacttt
     1141 atataatttg caaagtgctg gtttaaaaag tctcgtattt tattaaaagg gtttagtttt
     1201 ccttttttaa tccgttgatc aagctattca agtaaaagat aatattttct ttcgtgtacg
     1261 attttaacca agaatctctt ctttgaaatt tattctgaca ataaattgct ttttgccgaa
     1321 gaagatgtaa ctttgaacat tgttagtaga tttttcacct aaacaattgg agaatttttt
     1381 attaagcaaa ccttgaagcc tttttaaaac tttccgattt ttttatcatt tctaactaca
     1441 actttaatat tttttcaaaa atttacatga aattatgcat cgaccttcaa acataataat
     1501 taaaaacaaa cgtgcaaatt ttgaatatga aattttagat cgatatatcg ctggtattca
     1561 gctttttggc actgaaatta aatccataag agatggtaag gcaagtttga ctgataccta
     1621 ttgtatcttt attaatcatg aattatgggt aaagaacatg cacatcgctg cttattcttt
     1681 tggcacttat aataatcatg aagtccgccg tgatcgtaaa ttattattaa cgaagcgaga
     1741 actgctcaaa ctggaaagag ccaataaaga aagtggaaca actatcgtac cgctgaagct
     1801 ttttatcaat gaaaaggggt tagctaagtt ggaaattgcc ttagtgaggg gaaaaaaatt
     1861 gtacgacaaa cgtcaatccc tgaaagaaaa agaagatcaa agaaatatag agagagcctt
     1921 gaaacaaaaa gagcattgaa ttcgctgcat aaaaaatcat tttttgctca actaatttat
     1981 ttttactttt tctcaaaata caaaatattt tttacatttg ttaaagccat ttatacttca
     2041 tcaatgtaat gatgaaatta tcttgcttga cagatagtga aatatggctt tcttttaaga
     2101 aaggagaaga atgggcagtt tcctacattt acaaggaata ttctcccaaa ttatatcaat
     2161 atggggtaaa atttactgta aatgttccat tagtagaaga taccattcaa gatttattta
     2221 cagaattgat taaaaatcat aaaacgatag gtgagacaaa caatatcctt ttttatttat
     2281 taaaatcttt tcgacgaaaa ttgtttcgaa aaatggaaag cgaaaagaaa tattcaaatg
     2341 gcacagagcc agaagaatat caatttgaag tcgtatggtc tatagaacat gatttaatta
     2401 ttgaagaaga agcaaatcaa aagaaaacat cattattaaa agcccttgac aaactcacac
     2461 cccgtcaaaa agaagttatt taccttcgtt ttacaaaaga actagattac tgttctatag
     2521 ctgaaattat gaatattagt atagagtctt gtcgcaatct tatctcaaaa gctattttca
     2581 atataaaaaa aggtaaacgg gaaaaaataa aattcgcgat aaatattttt tacttaagtc
     2641 caaacggaag gaatattagt gaagtagttt ttgatgctat cttattatca gtcataatgt
     2701 aatttaatga tttccttatt tctaaatctt atctatccaa taaggttatt tttcaaattt
     2761 gctctgcatt ttagtagcgg gaacgagagt tgaactcgtg acctccgggt tatgaatccg
     2821 acg
//
LOCUS       DAOTKS010000212         2815 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_29125_length_2815_cov_3.976449, whole genome shotgun sequence.
ACCESSION   DAOTKS010000212 DAOTKS010000000
VERSION     DAOTKS010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29125_length_2815_cov_3.976449"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..263
                     /locus_tag="P5236_04860"
     CDS             9..263
                     /locus_tag="P5236_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63727.1"
                     /translation="MNEMQVKLKAEKIIEALTALSLGKNPNIFSGEIFRKLADHPNFI
                     AIRDAYIEYLKNFTGEINTQEDVKRLFDFRMKIVELFSQE"
     gene            936..1805
                     /locus_tag="P5236_04865"
     CDS             936..1805
                     /locus_tag="P5236_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HRU63728.1"
                     /translation="MKYFPPSELLINEDGSIFHLHIKPEQLADNVILVGDPGRVATVA
                     SYFDSQECSVSNREFKSITGTYKSKRITVISTGIGTDNIDIVLNELDALANIDLEKRT
                     EKPEFRQLTIVRIGTSGAMQPEIPLGSFLISEKSLGFDGLLSFYAGRDKVCDLDFEEA
                     FKKHVDWNPKLASPYVVDNDKELIERIGKDDMLKGVTISANGFYGPQGRVLRLDLHDS
                     HLNDKIESFRHNGYKITNFEMESSAIAGLSKLMGHKAMTVCCIIANRRIESANTNYQP
                     QMEKLIQLVLDRI"
     gene            1847..2422
                     /locus_tag="P5236_04870"
     CDS             1847..2422
                     /locus_tag="P5236_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016267357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HRU63729.1"
                     /translation="MITDKETYKYYLKSDLHAYQLSRITWLNFWKMDCLRFQCRLRKI
                     EYLFNVKKKNPFCKFYSLCLQILNHRLGTRLGLSIPKNVFGAGLCIVHYGTIVVSPFA
                     KIGKNCRIHPSTCIGEYNGAPTIGDNVYIGPGAKIFGNITIGNNVAIGANSVVNKNIP
                     DNVTIGGIPAKIISNKSSIDLGVFPENFFEK"
     gene            2419..>2815
                     /locus_tag="P5236_04875"
     CDS             2419..>2815
                     /locus_tag="P5236_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRU63730.1"
                     /translation="MNPTICAISTPPGSGGIAIIRLSGPEAISYANKIFMPKKESLDL
                     FQQQPYTVNFGSIVNKKNEIIDDVLVTVFRAPHSYTGEDVVEISCHGSIYIQQQILQI
                     LIEEGCSLAKPGEFTQRAFLNKKMDLSQAE"
BASE COUNT          968 a          406 c          475 g          966 t
ORIGIN      
        1 gttgatttat gaatgaaatg caggtgaagc tgaaagctga aaaaattatt gaagcattga
       61 cagctttaag tcttggtaaa aatcctaata ttttttctgg tgaaattttc agaaaattag
      121 ctgatcatcc taattttata gctattcgtg acgcttatat tgaatatctc aaaaatttta
      181 caggcgaaat taatacacaa gaggatgtca aacggctttt tgattttaga atgaaaattg
      241 ttgagttatt ttcgcaagaa tgaagcattg aaaattaggg aagaaagata atttaaatag
      301 ctttatatta ttatactatt tgacataata taaattatag gaaaactatg tagaaaattt
      361 atttcggtga cattctcctt ttttatgtga tgctttcaat tttattaaat ttacattaaa
      421 gttacactgg cgaaggtata tacctctaac tgtatatttt ttcttgttct ttggtcatgt
      481 ttaataaaca acaaatattt tgtgataaaa attcaaaata taataagaca ataaaatttt
      541 tagtgtgatt ttgtacccgc aaaaaatttt gataatttta ttcattttca atttgacctt
      601 aaaacctttt gttattaaaa ttttattaat gaataacccc gaggcaagcc tcgaggaatt
      661 ctttcgatta aattgattat aaccttatta ctttatttag ttgattatta atctcatatt
      721 atcacttgat ttttaaataa ttacccctcc cctactcttt tattttgaat aataccttaa
      781 attgttgtat ttcaatcatt taaaaatatt tttgagttat tttttataaa aagagcttaa
      841 atagccatta aaaagcaaaa attgaataaa aaaggttata tttgtggcaa ttaagcaaat
      901 ctatatttaa aaaaatacat taatttttta ccgttatgaa atatttccca ccttccgagc
      961 tgcttatcaa tgaagatggt agcatttttc acttgcatat caaacctgaa cagttggctg
     1021 acaatgtaat attggttggt gatcctggac gagttgctac agttgcttca tattttgatt
     1081 cacaagaatg ttcggtatcg aatcgtgaat ttaaaagcat tacaggtact tacaaatcca
     1141 aacgaattac ggtgatttcc actggaattg gtaccgataa cattgatatt gttttgaacg
     1201 agttggatgc tcttgctaat attgatttgg aaaaaagaac tgaaaaacca gaattcagac
     1261 aattgaccat tgttcgtatt ggcacttcag gagcaatgca acctgaaatt ccactgggaa
     1321 gttttttaat ttcagaaaaa tcgttgggat ttgatggtct tttaagtttt tatgctggta
     1381 gagataaagt ttgtgatctg gattttgaag aggctttcaa aaaacatgta gattggaatc
     1441 caaaattagc ttctccttat gtggttgaca atgataaaga attaattgaa agaattggta
     1501 aagatgatat gctgaaagga gtaaccattt cagccaatgg attttatgga cctcagggac
     1561 gtgttcttcg ccttgacttg catgatagtc atttgaatga taaaattgaa agttttcgac
     1621 ataatggcta taaaattact aattttgaaa tggaaagttc tgccattgct ggactatcga
     1681 aattaatggg tcacaaagca atgactgttt gttgtatcat agctaatcga agaattgaat
     1741 ctgccaacac gaattatcaa cctcaaatgg aaaaattgat tcaattggta ctggatagaa
     1801 tttaaaagaa tgtgaataat ttgctttgat cttcaacatt gcaaaaatga ttacagataa
     1861 agaaacatac aagtattatc tcaaaagcga tttgcatgcc tatcaattgt cacgtataac
     1921 atggcttaac ttttggaaaa tggactgcct gagatttcaa tgtcgattga gaaaaattga
     1981 atatcttttc aatgtaaaaa agaaaaaccc tttttgtaaa ttctactcac tttgcctgca
     2041 gatactcaac catcgcttag ggacaagatt aggtttatcc attccaaaaa atgtttttgg
     2101 agcagggctt tgtattgttc attatggtac tattgtagtg agtccatttg ccaaaatagg
     2161 taaaaattgt cgaattcatc cctccacttg cataggggaa tacaatggag ctccaactat
     2221 aggtgacaac gtatacatag gaccaggagc aaaaattttc ggtaacataa ccataggaaa
     2281 taacgttgcc ataggagcca attctgttgt aaataaaaat atacccgata acgtaacaat
     2341 cggaggcata cctgcaaaaa tcatatcaaa taaatcgtca atagatttag gtgttttccc
     2401 tgaaaatttt tttgaaaaat gaatccaact atttgtgcta tatcaacacc acctggttca
     2461 ggaggtattg ctattattcg actttctggt ccagaagcga tcagttatgc caataaaatt
     2521 ttcatgccga aaaaagaatc gcttgatctt tttcaacaac agccttatac ggttaatttt
     2581 ggtagtatag taaacaaaaa aaatgaaatt attgacgatg ttttagtcac tgtttttcgt
     2641 gctcctcatt cttacacggg tgaagatgtt gtggaaattt cttgtcatgg atctatttat
     2701 attcaacaac aaattctgca aattttgatt gaggaaggtt gttcgctggc aaaacctgga
     2761 gaatttacgc aaagagcttt tctgaacaag aaaatggatt tgtctcaagc cgaag
//
LOCUS       DAOTKS010000213         2809 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_29206_length_2809_cov_4.501089, whole genome shotgun sequence.
ACCESSION   DAOTKS010000213 DAOTKS010000000
VERSION     DAOTKS010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29206_length_2809_cov_4.501089"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="P5236_04880"
     CDS             <1..412
                     /locus_tag="P5236_04880"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HRU63731.1"
                     /translation="QKNVFKTDITLLINKKIDFQLMNEAAQLLKKYNDFTSFSKLHTH
                     VKTNYCTIYQAEWQQQDEEWIFIVEANRFLRNMVRSIVGTLFEIGFKKINIEQFKEII
                     EAKNRCKAGMSVPAKGLLLSEIKYPEKIFVVENN"
     gene            507..1262
                     /gene="hisF"
                     /locus_tag="P5236_04885"
     CDS             507..1262
                     /gene="hisF"
                     /locus_tag="P5236_04885"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HRU63732.1"
                     /translation="MLAKRIIPCLDVKDGKTVKGINFENIKNVGDPVELGAYYAQMGA
                     DELVFLDITATNEKRKTLSELVTRIAKEINIPFTVGGGISSIYDVSFLMACGADKISI
                     NTAAVLHPELITELAKNFGSQCIVLAIDTKFINGEWYVFLNGGRVPTDIKTIEWAKKA
                     VDLGAGEILLTSMDHDGTKNGFAIDITRAVSESVNVPVIASGGAGTMEHFVEVFKEGK
                     ADAALAASIFHYKEIEIPALKAYLKQKGIEVRL"
     gene            complement(1924..2697)
                     /locus_tag="P5236_04890"
     CDS             complement(1924..2697)
                     /locus_tag="P5236_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRU63733.1"
                     /translation="MKVTLIQDEIFWADKTTNLRKVGQQLEFLSGKTDLVILPEMFST
                     GFCTDQLNLAETMEGETIQMLRSYALKYQLAITGSFIATENGKFYNRAFFIFPTGEMV
                     TADKRHLFQAGGENKFFTKGNKRLQVSYYGFNICVLVCYDVRFPVWSRNVNNEYDLLI
                     YVANFPLKRINDWDVLLQARAIENQTYVAGVNRIGTDGLGIEYNGHSALLDFKGRPLL
                     AFPENEFSIQTTELDKAPLISYRERFPVWKDADIFEIKK"
BASE COUNT         1001 a          445 c          467 g          896 t
ORIGIN      
        1 tcaaaaaaat gtttttaaaa cagatataac tctactaata aataaaaaaa tcgactttca
       61 attgatgaat gaagcagctc aactcttgaa aaaatataat gattttacaa gtttcagtaa
      121 gttgcacacc catgtaaaaa ctaattattg tactatttat caagcagaat ggcagcaaca
      181 agatgaggaa tggattttta tagtagaagc caatcgtttc ctcagaaata tggtacgaag
      241 cattgtaggg actttgtttg aaattggatt taaaaagatt aatattgaac aatttaaaga
      301 aatcattgaa gcaaaaaatc gttgcaaagc aggaatgtct gtaccagcca aaggattatt
      361 gctttctgaa atcaaatatc cagaaaaaat ttttgtggtg gaaaataatt gatcaacatt
      421 atttgggtca aatttattaa ttttacctcc tcaatttata ggaaaatcaa catttagaat
      481 aaattaaaat ataattgtct atcaatatgt tagctaaacg aatcattcct tgtctggatg
      541 taaaagatgg aaaaacggta aaaggaataa atttcgaaaa cattaaaaac gtaggtgacc
      601 ctgtagaatt aggagcttat tatgcacaaa tgggagctga tgaactggtt tttttggata
      661 ttactgctac taacgaaaaa aggaaaacat tatcagagtt ggtaacacgg attgctaaag
      721 aaatcaatat accttttact gtaggaggag gcatttcaag tatttatgat gtttcatttt
      781 taatggcttg tggagccgat aaaatctcaa taaatacagc agcggttttg catccggaac
      841 ttattaccga gctggcaaaa aattttggca gtcaatgtat agttttagcc attgatacaa
      901 aatttatcaa tggagaatgg tatgtttttt tgaatggagg cagagttcca actgacatca
      961 aaactataga atgggcaaag aaagctgtcg atctgggtgc aggagaaatt ttactgactt
     1021 ctatggatca cgatgggacc aaaaatggtt ttgctattga tattaccaga gcggtttcag
     1081 aaagtgtcaa tgtccccgtc attgccagtg gtggagcagg tacgatggaa cattttgtag
     1141 aagtttttaa agaagggaaa gctgatgcgg cattggcagc cagtattttt cactataaag
     1201 aaatagaaat tcctgctctt aaagcttatt taaaacaaaa aggaatcgaa gtaagattgt
     1261 aatacatttt gtttcaaaaa aaatttatat agtttcgagt agagacattt caggaaacat
     1321 ctattgagtt ctgattcttt agtagagccg ttgcatgcaa cggctctact atttatatcc
     1381 cttgttcggc gtagtctcca gactacgtcg aataaaaata aaatttcttg attttaaccg
     1441 ctgataaaat caaacgtttt gctaaaccaa tatttgtcat aaaaaaacct tatcaaaaac
     1501 ctttaacctt cataataagg aatgttccct atagcttaca accttattac cttatttatc
     1561 aggtcatttt tcttttctct gaatgtaatt agcaacgccg ttcagaatta ttacctgtaa
     1621 tgtatatcaa aaaaggttcg tagcaccgat ttttttgtag ccccgaaaat ttcccaaacc
     1681 cacattttga ggtacagcat acgaaatgaa aaaatctcga tgtcccttcg tgtgataaac
     1741 cattcatttt caataagcta tattgagaaa atataaagtg ggacggagaa aaatttctat
     1801 tggaaagaat ttgcaatttt caataattta tgaggtttga ttttcacaat aaataaaata
     1861 tcagattaca aatccaacag aacaaaggat gaaaaaccaa aattcttttt acaattaaag
     1921 gatttatttt tttatttcaa aaatatctgc atctttccac acgggaaatc tttctctata
     1981 agaaattaaa ggagctttat ccagttcggt tgtttgaata gaaaattcat tttcaggaaa
     2041 agcaagaaga gggcgtcctt taaaatcgag taaagctgaa tgcccattgt attcaattcc
     2101 aagaccatca gtaccaattc tgtttacccc cgctacataa gtttgatttt caatagcacg
     2161 agcttgtaat aatacgtccc aatcattgat tcgcttcaag ggaaagttgg ccacataaat
     2221 caataaatca tattcattgt tcacatttcg tgaccaaaca ggaaaacgaa catcgtaaca
     2281 aaccaacaca caaatgttaa aaccataata gcttacctga agacgtttat tccctttagt
     2341 aaaaaattta ttttcgcctc ccgcttgaaa caaatgtcgt ttatcggctg ttaccatttc
     2401 accggtagga aaaataaaaa aggctcgatt ataaaattta ccattttctg tagcaataaa
     2461 acttccagtg atggctaatt ggtattttaa agcataagag cgaagcatct gtattgtttc
     2521 gccttccatt gtttcggcta aatttagttg atcggtacaa aagccggttg aaaacatttc
     2581 aggcaaaata accaaatctg tttttcctga gagaaattcc aattgttgac caacttttcg
     2641 taaattagtt gttttgtctg cccaaaaaat ttcatcttgt attaaagtaa ctttcataaa
     2701 caaatgatta aaaaattttt ctgttgtatt tctcaggata ttttggctga acactttcat
     2761 aaaaattctg aatttcttga aaatcagcat caatatttcc tgttggata
//
LOCUS       DAOTKS010000214         2801 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_29298_length_2801_cov_4.121996, whole genome shotgun sequence.
ACCESSION   DAOTKS010000214 DAOTKS010000000
VERSION     DAOTKS010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29298_length_2801_cov_4.121996"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1811
                     /locus_tag="P5236_04895"
     CDS             <1..1811
                     /locus_tag="P5236_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRU63734.1"
                     /translation="HFSYAEKNNKPVIFVMNHLDHEKVNYELSLESLTENFGNKVIQV
                     QYPFNPGLEFNAIIDLIQMKMLQWKTEGGNPEILEIPAEEMEKAKQLHNQLVEAAAEH
                     DETLMEKFFEEGILTEEEICNGLRKGVRQRGVFPVFCISALKNIGVSTLMDYLVKIAP
                     SVAESEWPETKDGKKVEPNPNAPTSLYVFKTNVEPHVGEISLFKVMSGTVHEGDDLYN
                     INRGVKEKINQLFNICGQIRNKVEEIGAGDIGATVKLKDTRTGNTLNGKGCDYDFKEI
                     NYPNPKYRRAVKAMSEADEEKLNEVLTRMHEEDPTWIVEQSKELKQTILHGQGEFHLR
                     TLKWRVENNDKIEIEFKEPKIPYRETITKQARADYRHKKQSGGAGQFGEVHLIIEPYS
                     EGLPIQDVYRFNGQEYKISVRDVQEIDLEWGGKLIFVNSIVGGAIDARFMPAILKGIM
                     ERMEQGPLTGSYARDVRVIVYDGKMHPVDSNEISFRLAGRNAFVRAFKEAGPKLLEPI
                     YDVEVLAPSDRLGDVMSDLQSRRAVIMGMHSEKGFEKLNAKVPLKELGNYSTTLSSLT
                     GGRATFNMKFASYELVPMEIQEKLLKEYEATRKEEE"
     gene            2016..2711
                     /locus_tag="P5236_04900"
     CDS             2016..2711
                     /locus_tag="P5236_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275865.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HRU63735.1"
                     /translation="MKNRIVESSIIAVGIICLGLFIQNGIKIFSQKDRAVEVKGLAEM
                     EVPANKVICPILYKDLGNDLAQLYIELNKKNKAIISFLKENGIHDNEISVSAPEIVDM
                     EAERFIENKSPYRYNLTSVITITTENVDKVRALMTRQAELLKQGIAVTVNNYSVRYEY
                     TKLNEIKPQMIEEATKNARKAAEKFAKDSGSKLGKIKNATQGQFVISDRDENTPYIKN
                     IRVVSTITYYLED"
BASE COUNT         1037 a          445 c          534 g          785 t
ORIGIN      
        1 accacttttc atatgctgaa aaaaataata agcctgtaat ttttgtaatg aatcatttag
       61 atcatgaaaa agttaattac gaactctcat tagaatcact aacagaaaat tttggcaaca
      121 aagtcattca agtacaatat cccttcaatc caggattaga attcaatgcc attattgatt
      181 taattcaaat gaaaatgctg caatggaaaa ccgaaggtgg caatcccgaa attcttgaaa
      241 ttcctgctga agaaatggaa aaagccaaac agcttcataa ccagttggta gaagcagccg
      301 ccgagcatga tgaaacttta atggaaaaat tttttgaaga aggcattctt actgaagaag
      361 aaatttgtaa tggtcttcgc aagggggtac gtcaaagagg agttttccct gttttttgta
      421 tctcagcatt aaaaaatatt ggagtaagca ctttaatgga ttatttggta aaaattgctc
      481 ccagtgttgc cgaaagtgaa tggcctgaga ccaaagatgg gaaaaaagta gaaccaaatc
      541 ccaatgcccc aacaagtctt tacgtattta aaaccaatgt agaacctcat gttggagaaa
      601 tttccttatt taaagtgatg agcgggacgg tacatgaagg tgacgattta tataacatta
      661 atagaggagt aaaagaaaaa attaatcaac tttttaatat ttgtggacaa attcgcaaca
      721 aagtagaaga aattggtgca ggcgatatag gtgctaccgt taaattgaaa gacacacgta
      781 ccggtaacac cttaaatgga aaaggatgcg attatgattt taaagaaata aattatccta
      841 atccaaagta ccgtcgagca gtaaaagcaa tgtcggaagc tgatgaagaa aaattaaatg
      901 aagttttgac ccgcatgcac gaagaagatc ctacctggat tgtagaacag tcaaaagaac
      961 ttaagcaaac tattttgcat ggtcaaggtg aattccatct tcgtacttta aagtggagag
     1021 ttgaaaataa tgataaaatt gaaatagaat ttaaagaacc taaaatacct tatcgcgaga
     1081 ccatcaccaa acaagcacga gctgattatc gccacaaaaa acaatcgggt ggtgcaggtc
     1141 aatttgggga agtacattta attattgaac cttattctga aggcttacct atccaagatg
     1201 tttatcgttt caatggacaa gaatataaaa ttagtgttcg tgatgttcaa gaaattgatc
     1261 tggaatgggg aggaaaattg atttttgtca acagtattgt cggtggagcc attgatgcac
     1321 gatttatgcc ggctatatta aaaggaatca tggagcgtat ggaacaagga cccttgacgg
     1381 gctcgtatgc tcgagatgta agagtgattg tttacgacgg taaaatgcat cctgtagatt
     1441 ccaatgaaat ttcattccgt ttggcaggaa gaaatgcttt tgtacgagcc tttaaagaag
     1501 caggtcctaa actattagaa cccatttacg atgtagaagt acttgcaccg tcagatagat
     1561 tgggcgacgt catgagtgat ttgcaaagcc gacgggcagt tatcatggga atgcatagcg
     1621 aaaaaggttt tgaaaaatta aatgcaaaag tacctctgaa ggaattagga aattattcta
     1681 ccacacttag ttcactcact ggaggaagag caaccttcaa catgaaattt gccagttatg
     1741 aactcgtccc aatggaaatt caggaaaaat tactgaagga atacgaagca actcgcaaag
     1801 aagaagaata aatgtggtaa atccaaaata ttaattcaaa tgagttatta attaatcgtt
     1861 aattaatttg atgtattcta ttaattatca ataaaataac aataaaatat aggtgaacag
     1921 tgaatgttaa actttttaag gattaataac gtaaaaacat cttgaattta tcctttaaaa
     1981 cagtaatact ctttcacaaa tgaaaaacca attccatgaa aaatagaatc gttgaatcat
     2041 ctattattgc tgtaggaatt atttgcttgg gactattcat tcaaaatggc atcaaaattt
     2101 tttcccaaaa agaccgtgcg gtagaagtta aagggcttgc tgaaatggaa gtcccagcca
     2161 ataaagtaat ttgccccatt ctatacaaag atttgggaaa tgaccttgct cagctttata
     2221 ttgaactgaa taaaaagaat aaagccatta tttcattttt gaaagaaaat ggtatccatg
     2281 acaatgaaat atccgtttct gctcctgaaa ttgtggatat ggaagccgaa agatttatcg
     2341 aaaataaatc accttatcgt tataatctga catctgttat taccatcaca accgaaaatg
     2401 ttgataaagt acgtgctttg atgactcgcc aagctgaact ccttaagcaa ggaattgctg
     2461 ttacggtgaa caattacagc gttcgctatg aatatactaa attgaatgaa attaagccac
     2521 aaatgattga agaagcgaca aagaatgcac ggaaagcggc tgaaaaattt gccaaagatt
     2581 cgggcagtaa attaggaaaa atcaaaaacg ctacccaagg acagtttgtt atttccgaca
     2641 gagatgaaaa tacaccttac atcaaaaata ttcgcgtagt ttcaaccatt acttattacc
     2701 tggaagattg aaatcagaat agagcaattc ttctcctctt cattttcaga attttgccta
     2761 tggcttattt tgaaaaaaat tattgacaac atagaaaagg t
//
LOCUS       DAOTKS010000215         2797 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_29354_length_2797_cov_3.825675, whole genome shotgun sequence.
ACCESSION   DAOTKS010000215 DAOTKS010000000
VERSION     DAOTKS010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29354_length_2797_cov_3.825675"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="P5236_04905"
     CDS             <1..193
                     /locus_tag="P5236_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HRU63736.1"
                     /translation="HVARGIFRTSSHKILIPACVLCGASLLMICDIISQIPAYSLPIN
                     TVSALFGAPVILWIILKRK"
     gene            241..1230
                     /locus_tag="P5236_04910"
     CDS             241..1230
                     /locus_tag="P5236_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRU63737.1"
                     /translation="MCILSTVDLTIGYLKKDKKKVVQSNLNLELYAGKLTGLIGPNGV
                     GKSTLLRTLSGLQKPLGGKVLLENKELSKLSYQEKATKIALVLTDKIDILNATVYDIV
                     SYGRYPYSNWLGNLTDKDHIYIEQAIQMVHLEEKRNELLNELSDGERQRVMIARALAQ
                     DTPVILLDEPTSFLDVSNRIEIMLLLHKLAHQTQKAILLSTHELDLALQAADSIWLMD
                     KNGKTICGVPEDLVFNGSFARAFASKYYFFNEANGNFSMNFSLNKQVAVTGNKTRMYW
                     TLRALARAGFEAKEEAPVKILVTEDGWILNDEKINSMEELLDKLTELEKLNLS"
     gene            1337..>2797
                     /locus_tag="P5236_04915"
     CDS             1337..>2797
                     /locus_tag="P5236_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRU63738.1"
                     /translation="MNKRLSFLFCFFVVTLILTAQNKVRIFGYVVDPNNRGIELVNVF
                     LKGTTTGTTTNQNGYYELITEIKDSAEIIFSMVGYQTVSYTIHPKAKVIQLTVELPAL
                     NQEIKEIDIIGQKRQTNTIDVLNPSKYRLMPNPSGGIESLLIAFTGVTSSNELSSQYN
                     VRGGNFDENIVYVNGIEIYRPLLIRAGQQEGLSFINSDMVQNVSFSSGGFGVEYGDKM
                     SSVLDIQYKKPTRTEASVSLNLLGASAYVGTASKRFTQMHGIRYKTSAYLLGTLETKG
                     EYNPSFVDYQTYMTYQLKPKWELTFLGNFSQNSYQFIPQTRETSFGTYNMGRKLTVFF
                     GGQEKDLFQTAFGAFTLNYHPLKETKLSLIASGFRTNEKETYDISGEYILSEIKMEDD
                     LSKETGETLGIGNYHEHARNRLNATVMNISHQGESKAGNHQLKWGALLQFEKINDKIN
                     EWEWRDSSGYSLPYHPYDVKLYYNLKALNSLSSTRSS"
BASE COUNT          932 a          418 c          531 g          916 t
ORIGIN      
        1 tcatgttgcc agaggaattt ttcgaacttc ttctcataaa attttaattc ctgcttgtgt
       61 gctttgtggt gcttcgctgc tgatgatttg tgatattatt tctcaaatac cagcttattc
      121 gttgcctatc aatacagtaa gtgcattgtt tggtgcacct gttattttgt ggattatttt
      181 aaaaagaaaa taagaaaatt gttttttcat ttattgctgg aaaattattg attgtgagtt
      241 atgtgcattt tgtccactgt tgatttaacg attggttatt taaaaaaaga taaaaaaaaa
      301 gtagtacaat ccaacttgaa tttggagttg tatgccggga aattaacagg attaatcggt
      361 ccaaacggcg taggaaaatc tactttattg cgaactttat cgggcttgca aaaaccactc
      421 ggtggaaaag ttttgcttga aaataaggaa ttatctaaac tttcttatca agaaaaagca
      481 acaaaaatag ctcttgtttt gactgataaa attgacatat taaatgctac ggtttatgat
      541 attgtttctt atggtcgata tccttacagc aattggctgg gaaatttaac tgataaagat
      601 cacatatata ttgaacaggc aattcagatg gtgcatttag aagaaaaaag gaatgaactg
      661 ttaaatgaac tttcggatgg tgaacgtcaa agggtaatga ttgccagagc attggctcag
      721 gatactcctg taattttatt ggatgaacca acctcatttc tggatgtctc caatcgaata
      781 gaaattatgt tgttgttaca caaattggct catcaaacgc agaaagcaat tttgctttcc
      841 actcacgaac ttgatttggc cttacaggct gcggatagta tctggttgat ggataaaaat
      901 ggaaaaacga tttgtggtgt gccggaagat ttggttttta atgggagctt tgctcgagct
      961 tttgccagta agtattattt ttttaatgaa gccaatggta atttttccat gaacttttct
     1021 ttgaacaaac aagtggcagt gacaggaaac aaaaccagaa tgtattggac actcagagca
     1081 ttggcacgag caggttttga agcaaaagaa gaagcaccag taaaaattct ggttaccgaa
     1141 gatggttgga ttttgaatga tgaaaaaata aactctatgg aagaattgct tgataaattg
     1201 acggaattgg agaaattaaa tttatcataa aaaacatttt ttacaaatca ttcaaaagaa
     1261 gatagaaagg aaaaataaaa taatcattcc cttttttcgt ttttagggta atttatttct
     1321 atatttaata cattgcatga acaagcgatt gagctttctt ttttgttttt ttgttgttac
     1381 gctgatttta accgcacaaa ataaggttcg gatttttggc tatgttgttg atcctaacaa
     1441 caggggaatt gaactggtaa atgttttttt gaaaggtaca acaacaggaa ctaccaccaa
     1501 ccaaaatggc tattatgaat tgatcaccga aattaaagat tcggctgaga ttattttttc
     1561 gatggtcggt tatcagactg tttcttatac cattcatcca aaagcaaaag ttattcaatt
     1621 aactgttgaa ttaccagctc tgaatcaaga aataaaagaa atagatatta ttgggcaaaa
     1681 acgtcaaaca aatactattg acgtattaaa tccttccaaa tatcgattga tgcctaatcc
     1741 ttcggggggt attgaatctt tattgattgc ttttacagga gttacttcat caaatgaact
     1801 tagttcccaa tataacgtta gaggtggaaa tttcgatgaa aatattgtat atgtcaatgg
     1861 catagaaatt tatcgtccgt tgttaataag ggcaggtcaa caagaaggat tgagtttcat
     1921 caattcagat atggtgcaga atgtgtcttt ttcttcaggt ggattcggcg tagaatatgg
     1981 tgataaaatg tcatccgtgc tggatattca atacaaaaaa ccgacaagaa cggaagcttc
     2041 tgtttcgctt aatttattag gtgcttctgc ttatgtgggc acagccagca aacgttttac
     2101 tcaaatgcat ggcatccgat ataaaacttc tgcctatttg cttggaacgc tggaaacaaa
     2161 aggcgaatat aatccttcat ttgtagatta tcaaacttat atgacctatc agttgaagcc
     2221 caaatgggaa ttaacttttc ttggaaattt ttcacaaaac tcctatcaat ttattccaca
     2281 aaccagagaa acatcttttg gtacttacaa tatgggaaga aaactcaccg tattttttgg
     2341 cggacaggaa aaggatttat ttcaaacagc ttttggagct tttacgttga attatcatcc
     2401 tttaaaagaa actaaattga gtttaattgc ttcgggcttt cgtaccaacg aaaaagaaac
     2461 ttatgatatt tctggagaat atattttgag tgaaataaaa atggaagatg atttatcaaa
     2521 agaaacaggc gaaacattgg gaattggtaa ttaccatgaa catgctcgta atcgtttgaa
     2581 tgcaacggta atgaatattt ctcatcaagg agaatcgaaa gcaggcaatc atcaattaaa
     2641 atggggagca cttcttcagt ttgaaaaaat caatgataaa atcaacgaat gggaatggcg
     2701 cgattcgtcg ggttattcgt taccttatca tccttacgat gtaaaattat attataatct
     2761 gaaagcttta aattccttgt catctactcg ttcatca
//
LOCUS       DAOTKS010000216         2793 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_29410_length_2793_cov_6.010226, whole genome shotgun sequence.
ACCESSION   DAOTKS010000216 DAOTKS010000000
VERSION     DAOTKS010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29410_length_2793_cov_6.010226"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..352
                     /locus_tag="P5236_04920"
     CDS             26..352
                     /locus_tag="P5236_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HRU63739.1"
                     /translation="MTIKEAQQQVDNWIKKYGVRYFSELTNMVLLTEEVGELARIIAR
                     TYGEQSSKAEEEKTNLSDEMADVLWVLICLANQTGIDLTEAFEKNIKKKTERDAQRHQ
                     QNKKLH"
     gene            374..1258
                     /gene="deoC"
                     /locus_tag="P5236_04925"
     CDS             374..1258
                     /gene="deoC"
                     /locus_tag="P5236_04925"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRU63740.1"
                     /translation="MKDKYQELFDFYHCEVNDEAVKIEIDKILAEHFAENANKEVYNQ
                     CLNLIDLTSLNCTDTESEIATLTEKVNNFKNVFPHLSNVAAICVYPALVPVVKKHLND
                     QKVKIASVAGGFPASQTFLEVKIAEASMAVQAGANEIDMVISVGKFLEEKYEEVYDEL
                     SEIKATCRDAHLKVILETGALPNCTNIKKAALLAMASGADFVKTSTGKIQVSATLEAT
                     YTICHAIKEWYGKTGSKIGYKPAGGISTTEEAVKHFTLVKHILGNEWLNNQWFRFGAS
                     RLANQLLSSIEGKEIKYF"
     gene            complement(1259..2233)
                     /locus_tag="P5236_04930"
     CDS             complement(1259..2233)
                     /locus_tag="P5236_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRU63741.1"
                     /translation="MNVIEIIKKPIIKELHSFEKQLVNELKTENPLLTNINEYIQQGK
                     GKQLRPILMFLSAKICGAINEQTIISAISIELLHTASLIHDDVVDNAFERRGRPSVNA
                     RWDNKIAILAGDYLLSKSLNCAIKANNLEILHVIAKIGVELTDGELLQLENMQKIKIT
                     EDDYYKIIRKKTALLFSSCCIIGGLSVNANQTLLKKLKNFGEYVGICFQIKDDIFDYS
                     DSKNIGKPRRNDIREGKITLPLIYALQQADEKKKEKIINWIKKKKISEKNIEKITNFV
                     YKNGGIEYATSKLIEFKNKALTELDSFEESEIKQSLKYFAEYMVTREE"
     gene            2411..>2793
                     /locus_tag="P5236_04935"
     CDS             2411..>2793
                     /locus_tag="P5236_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HRU63742.1"
                     /translation="MEKKKLFLVDAHALIYRSYYAFIRNPRINSKGQNTSAIFGFVNT
                     LEEVLKKEQPTHMAVAFDPEGKTFRHEAFEQYKAQREMTPEDIRKAIPIVKELVDAYN
                     IPILEVPGYEADDVIGTIAKQAEKAG"
BASE COUNT          959 a          422 c          462 g          950 t
ORIGIN      
        1 attgctcgtt taaaagaaaa taaagatgac aatcaaggaa gctcaacagc aagttgataa
       61 ttggataaaa aaatatggcg ttcgttattt tagcgaatta accaacatgg ttcttctaac
      121 cgaagaagtc ggagaactcg ctcgtattat tgcccgtact tatggcgaac aatcatcgaa
      181 agcagaagaa gaaaaaacaa atttatctga tgaaatggca gatgtattgt gggtattgat
      241 ttgtctcgcc aatcaaacag gaattgacct tactgaggct tttgaaaaaa atattaaaaa
      301 gaaaacagaa agagatgctc aacggcatca gcaaaataaa aagttgcatt aaatatttaa
      361 aaatcaattt attatgaaag acaagtatca ggaattattt gatttttacc attgtgaagt
      421 caatgatgaa gctgtaaaaa ttgagattga taaaatattg gctgaacatt ttgctgaaaa
      481 tgctaacaag gaagtttata atcaatgttt aaatctgata gatttaactt cgttaaattg
      541 taccgataca gaaagtgaaa ttgctacgtt gacagaaaag gtaaataatt ttaaaaatgt
      601 tttcccgcat ttatccaatg tagctgccat ttgcgtttat ccagcattag tacctgttgt
      661 aaaaaagcat ttgaatgatc aaaaagtgaa aatagcatca gtagctggtg gttttcctgc
      721 ttctcaaact tttcttgaag tgaaaattgc tgaagcttca atggctgttc aagcaggtgc
      781 aaacgaaata gatatggtaa tttctgtagg aaaatttctt gaagaaaaat atgaagaagt
      841 ttatgatgag ttatcagaaa taaaagcaac ctgtcgggat gctcatttaa aagtgattct
      901 tgaaacaggg gctttaccaa attgtacgaa tataaaaaag gctgctttat tagcgatggc
      961 atcgggagct gattttgtta aaacttctac aggaaaaatt caggtttcag ctacactcga
     1021 agctacttat actatttgtc atgcgatcaa agaatggtat gggaaaaccg gaagtaaaat
     1081 aggttataaa ccagcaggtg gaatttcaac taccgaagaa gctgtgaaac attttacgct
     1141 cgtaaaacat attcttggca acgaatggct taataatcaa tggtttcgtt ttggagcaag
     1201 ccgtttagcc aatcaattgc tcagttccat agaaggaaaa gaaataaaat acttttaatt
     1261 attcctcacg tgtaaccata tattcagcaa aatatttcaa tgattgtttt atttcacttt
     1321 cttcaaagct atcaagttcg gtgagagctt tatttttaaa ttcaatcagt tttgaagtag
     1381 catactcaat gccaccgttt ttataaacaa aattcgtaat tttttctatg ttcttttcag
     1441 aaattttttt cttctttatc cagttgataa ttttttcttt tttcttttcg tcggcttgtt
     1501 gtaaagcata aattaaggga agggttattt ttccttctct aatatcattc cttcttggct
     1561 taccaatgtt tttgctatca gagtagtcaa aaatatcatc tttaatttgg aaacaaattc
     1621 ctacatattc cccaaaattt tttaattttt ttaagagcgt ttggttggca tttactgata
     1681 gacctccaat tatgcagcaa gaagaaaata aaagagcagt tttttttcga attattttat
     1741 aataatcatc ttctgtgatc ttaatttttt gcatgttctc taattgtaat aattcgccat
     1801 cggttaattc caccccgatt ttggcaataa catgtaaaat ttccagatta tttgctttta
     1861 tagcacaatt caatgatttt gataacagat aatcacctgc taaaatagca attttattat
     1921 cccaacgagc atttactgac ggtcttccgc gtcgttcaaa agcgttgtcg actacatcat
     1981 catgaattaa actggcagta tgtaacaact ctattgaaat agctgaaata attgtttgtt
     2041 cattaatagc tccacaaatt ttagcagata aaaacattaa aataggacgc aattgtttcc
     2101 cttttccttg ttgaatatat tcattgatgt tagttaacaa aggattctct gttttcaatt
     2161 cattaacaag ttgtttttca aatgaatgta gttccttgat aataggtttt ttaataattt
     2221 ctattacatt catgggagta atttattgaa atgtctacaa aaataatcaa tttcaatgtc
     2281 tgttttacca taaaatattc atttctgaga tttttttaat taattttaca aacaaaatga
     2341 atcatcaaaa agcaattttt attagcattt gcttttctag aagtgttata cttacaatat
     2401 ttgaatatca atggaaaaga aaaaactttt tttagtcgat gctcatgctc ttatttatcg
     2461 ttcgtattat gcttttattc gcaatccacg tattaattca aaaggtcaga atacttcagc
     2521 aatttttggt tttgtaaata ccctggaaga agttttaaaa aaagaacaac ccactcatat
     2581 ggcagtggct tttgatcctg aaggtaagac ctttcgtcac gaagcttttg agcaatacaa
     2641 agcacagcgt gaaatgacac cggaagatat tcgaaaggca atacctatcg taaaagaatt
     2701 ggtggatgct tataatattc ccattctgga agttcctggt tatgaagctg atgatgtaat
     2761 aggaaccatt gcaaaacaag cagaaaaagc tgg
//
LOCUS       DAOTKS010000217         2793 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_29412_length_2793_cov_4.504748, whole genome shotgun sequence.
ACCESSION   DAOTKS010000217 DAOTKS010000000
VERSION     DAOTKS010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29412_length_2793_cov_4.504748"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="P5236_04940"
     CDS             <1..384
                     /locus_tag="P5236_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63743.1"
                     /translation="SPQFGDVIVDIRAVDFPPVISISSQLSNSSAIFFNVLIIYFFKC
                     FLSTRFAFRNPRPLFHTATRELISGEITAPSYWLLFSLSVATGKKITMFFYYLCNAFS
                     CGICDTSGLLPQILLFAFLAREGFL"
     gene            complement(397..546)
                     /locus_tag="P5236_04945"
     CDS             complement(397..546)
                     /locus_tag="P5236_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63744.1"
                     /translation="MKKILFLKEDDLLSFTHLSRINGGSNNIYENEMVIIYINGKPVI
                     IKKEQ"
     gene            783..2174
                     /locus_tag="P5236_04950"
     CDS             783..2174
                     /locus_tag="P5236_04950"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRU63745.1"
                     /translation="MKRNVLILFSGVAIVLLIVSQIIWIQQLMAREKQRLEMELKQTL
                     QSIVAFCLSREVGNKTTQMLSLELIPIDPDKIPKNAVVKGSFDTKEYSSDKNLGNYLV
                     GVFAEDLLQENKIPLEPIDSLFRKEFAFYPEISEYSMSIEKNDSILRKLYVGENTCSV
                     LKDANSGVNIRIPIGKTGKYWYHAQVIFKPTIFTQRLQLMTALSAIAVIVISVLLLYQ
                     LALLRQQTRELAIHKHVAMGIVHDLKSPLAYVYTLLGFFEKSENEVQKKGMFYSAKVR
                     VKYLSEKIEILLSVMKSKEHFFQIHCKPYDFNLRCKEIMEELCVIYKGKDIAWCLEPA
                     EGTILSVDPVYFDGCVRNLLDNAVKYSGDKPFIKILTIDHNKHIFLSFADNGKGILKK
                     ERKKIFREFYRHDKSSSVKNHGIGLAFTRQVVRAHSGKIFVDSKEGEGSIFTIVFPKK
                     IAEKYGTNKSNIG"
     gene            2146..>2793
                     /locus_tag="P5236_04955"
     CDS             2146..>2793
                     /locus_tag="P5236_04955"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRU63746.1"
                     /translation="MEQIKVILVDDDTDLGNLISLSLSSDGYKVHYQNSLAGIEAIIQ
                     EFEPSILVLDVEVGEDDGIEKAKEIILTHPSLPILFISSHTDIESINRGISTGGVHYL
                     RKPFEIQELEIYINRFALSENHKEIIAAIGSYSLNFDSRELFYKHTYIKQLSPLEYSV
                     LILLFQNRNEVIANDTLSEKVWSKKFPDTEASINNVISKLRKLLKNDPNVCIQTIK"
BASE COUNT          910 a          438 c          484 g          961 t
ORIGIN      
        1 tcaccacaat ttggagacgt tattgttgat ataagggctg tagattttcc gcctgttatc
       61 tcaataagtt cacagcttag caattcttct gctatatttt ttaatgttct cataatttat
      121 ttttttaaat gtttcctttc tactcgtttc gcttttcgaa atccgcgtcc attgtttcat
      181 acagcaaccc gagaattgat ttcgggggaa attactgcac catcctactg gctacttttt
      241 tcactttcag tggctactgg taaaaaaatc accatgtttt tttattatct ttgcaatgcc
      301 ttttcttgtg ggatttgtga tacatctggt ttgttacctc aaattttatt gttcgctttc
      361 ctggcaaggg aaggcttttt gtaattataa gtttcgtcat tgttcttttt taattatgac
      421 gggtttacca tttatgtaaa ttattaccat ttcattttcg taaatattgt tacttcctcc
      481 atttattcgt gacaaatgag taaaagacaa taaatcgtct tcttttaaga ataaaatttt
      541 tttcataatc gataatattt atttaaggtt tattttacaa aagtaaaatg agttattgaa
      601 tctgtttcta accaacgtct aacatttgtc taaccaatct aaccaaaata attaaattaa
      661 caatctttaa ctaatcgatt tttttattcc caaacaagtg gaaaaattca attaattcat
      721 ctgcatgtaa atcttttttg ttttttactt acatttgcac ttacattaca aaatactaat
      781 acatgaaacg gaatgtactg attctatttt ccggtgtagc tattgtacta ctcatcgttt
      841 cgcagattat atggatacaa caattgatgg cacgcgagaa acaacgtctc gaaatggaat
      901 tgaagcaaac ccttcaaagt attgtggcat tttgtttatc gcgagaagtt gggaataaaa
      961 ccacacaaat gttaagtttg gaattgattc caatagatcc ggataaaatt ccaaaaaatg
     1021 ctgtcgtcaa aggttcgttt gatacaaaag aatattcttc cgataaaaat ttgggaaatt
     1081 atttggttgg cgtttttgcc gaagatcttt tgcaggaaaa caaaattccg ctcgagccca
     1141 ttgactcact tttcagaaaa gaatttgcct tttatccaga gatttctgaa tattccatgt
     1201 ctatcgaaaa aaatgattcg atattgcgta agttatacgt aggagaaaat acatgttctg
     1261 tcttgaagga tgcaaacagc ggagtaaaca taagaattcc cataggaaaa acaggtaagt
     1321 actggtatca tgctcaggta atattcaaac caactatttt cacacaacgg cttcagttga
     1381 tgactgctct ttcagctata gcggtgattg taatttcagt gttgttgctt tatcaattgg
     1441 ctttgttgag acaacaaaca cgggaactgg caatacataa acatgtagca atgggtattg
     1501 tccacgacct gaaatcgcca cttgcttatg tctatacctt gctgggcttt tttgaaaaaa
     1561 gtgaaaatga agttcaaaag aaaggaatgt tttactcggc taaggtgcgt gtaaaatatt
     1621 tatcagaaaa aattgagatt cttttatcag tgatgaagag taaggaacat ttctttcaga
     1681 tacattgcaa accttatgat tttaacttgc gttgcaagga aataatggaa gaactctgtg
     1741 taatttacaa ggggaaagat attgcatggt gtttagaacc tgctgaagga acaatacttt
     1801 cggtagatcc tgtttacttc gacggctgtg taagaaattt attggacaat gccgtgaaat
     1861 attctggcga caaaccattc ataaagattt tgactattga tcacaataaa cacatttttc
     1921 tttcttttgc cgacaatggg aaaggtattt taaaaaagga acgaaagaaa atatttcgtg
     1981 aattttaccg tcacgataaa agttcatcgg taaaaaatca tggtatagga cttgctttta
     2041 ccagacaagt tgttcgagca cattccggaa aaattttcgt cgatagtaaa gaaggagaag
     2101 gaagtatttt tactattgtt ttccccaaaa aaattgctga aaaatatgga acaaataaaa
     2161 gtaatattgg ttgacgatga taccgatttg ggcaatttaa taagtttatc tctttcttct
     2221 gatggttata aagtgcacta ccagaattct ttggcaggaa ttgaagccat tattcaggaa
     2281 tttgaacctt ccattttagt attggatgtt gaggtaggtg aggatgatgg tatagaaaag
     2341 gcaaaagaaa ttatcttgac tcatccctcg ttgccaattt tgtttatttc ctcacatacc
     2401 gacatagaaa gtattaacag aggtatttct actggtggtg ttcattatct tcgaaaacct
     2461 ttcgagatac aggaactgga aatctatata aatcgttttg ctctttctga aaatcataaa
     2521 gaaattattg cagcaattgg cagttattct ttgaattttg atagccgaga acttttttac
     2581 aaacatactt atatcaaaca gcttagtcct ctggaatata gcgtattaat actattgttt
     2641 caaaatagaa atgaagtgat tgctaatgat actctttctg aaaaagtatg gagtaaaaag
     2701 tttcctgata cagaagcgtc aataaacaat gtgatatcga aattaagaaa attattgaaa
     2761 aatgatccta atgtgtgtat ccaaacaata aaa
//
LOCUS       DAOTKS010000218         2766 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_29757_length_2766_cov_3.793065, whole genome shotgun sequence.
ACCESSION   DAOTKS010000218 DAOTKS010000000
VERSION     DAOTKS010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29757_length_2766_cov_3.793065"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2766
                     /locus_tag="P5236_04960"
     CDS             <1..>2766
                     /locus_tag="P5236_04960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRU63747.1"
                     /translation="TPQFHLRKVNKNISSLTANEVRAFLQTSDNYIIIATKDGSIHFF
                     DEKLNELGILSQNGQLNSGNKINDMAYCMFEDSKKNIWIGTKGGGVLKLIPRDSNNKI
                     PSYQIQRFENNPLDNNSLSNNNIYSIIEDKNGHILIGTFGGGLNILTEGKEGVSFINF
                     KNKLKNYPISTCPKIRHLLLDKNNTLWVATSNGLLQMDDFLTSKMKIYHIEKLPNMSA
                     SLSNNDVHYLHSDKKGQLWIATFGGGLLKLKDKATDHSPATFVNYSNKEGIYNDIILS
                     IQEDEQGNLWLSSENSISRFEPSTHYFQNYQILNGINDVFFSEAASLFTRKGEMLFGC
                     NKGFYYFIPRQIKKSKEIPPIEFTRLQLFNQDVKIREKNSPLKQSITYSNIIELTHKQ
                     SVFSIEYAALDYVNPEKIQYAFKLENFDKDWNYVQNQRKATYTNLPKGTYYFRVKSTN
                     NEGAMVNNEKMITIKILPSFWETSVAYVLYGIVFLLLLYTVYYLTSIYLKLKNEVIIE
                     QKVSEIKLRFFTNISHELRTPLTLIIGPIENILKNEKLSTTTHEQLKIVYNNTNRMMR
                     LINQILDFRKVQNNKMRLKVQPIYLVPFLQNICSNFKEEAQEKNMDFKFVNNAGDVVL
                     WLDMEKVDIIFYNLLSNAFKFTPNNKKIEVRIEQSNNNEDIQIKVIDEGIGIPKEKKP
                     FLYERFTSHNEINTLSNLYGSGIGLNLVKEFVDLHKGTIEVESEPGEGSTFTVVFHKG
                     KDHFGDEVDILEEKSNVLLPVDESLIDQNDAKISIEELESYYSTASKEAPFILVIEDD
                     KDMRHFLKMILSKNYRVESAIDGVDGWGKILSLFPDLVISDLMMPNKDGLELIQLIKD
                     NEKTSHIPVILLTEKNAVESKLQAYQYGADDYITKPFSPILLEARVENLLQQRKKLQE
                     NYRKRLF"
BASE COUNT         1057 a          421 c          438 g          850 t
ORIGIN      
        1 acactccgca attccattta agaaaagtaa acaaaaatat ctcgtcgctt accgctaatg
       61 aagtaagagc ttttttacaa acttcagaca attatatcat tattgcgact aaagatggtt
      121 ctatccattt ttttgatgaa aaattgaacg aattaggtat tctgtctcaa aatggacaat
      181 taaattctgg aaataaaatt aatgacatgg cttattgtat gtttgaagac agcaaaaaaa
      241 atatttggat tggtaccaaa ggcggaggtg tattaaaatt aattccacga gatagtaaca
      301 acaaaattcc cagctatcaa atacaacgat ttgaaaataa ccctcttgac aacaatagct
      361 tatctaacaa taatatttac agtattattg aagacaaaaa tggacatata cttattggaa
      421 cttttggtgg aggattaaat attcttactg aaggaaaaga aggcgtttct ttcatcaatt
      481 tcaaaaacaa attaaaaaat tatcctatta gtacctgtcc gaaaattcgc catttactgc
      541 tggataagaa taacacactt tgggtagcga cttcaaacgg acttttacaa atggacgatt
      601 ttttgacctc aaaaatgaaa atatatcaca ttgaaaaatt gcctaatatg agtgccagtt
      661 taagtaataa tgatgtgcac tatcttcatt cagataaaaa aggtcaacta tggattgcta
      721 cttttggtgg aggtttactc aagctcaaag acaaagctac ggaccattca cctgctactt
      781 ttgtcaatta ttccaacaag gaagggatat ataatgacat tattctcagt attcaagaag
      841 atgaacaagg caatttgtgg ttaagttcag aaaacagtat ttctcgattt gaaccatcga
      901 cacattattt tcaaaattat caaatcctga acggaattaa tgatgtgttt ttttcagaag
      961 cagcaagctt atttaccaga aagggcgaga tgcttttcgg atgtaataag ggattttatt
     1021 attttattcc tcgacagatt aaaaaaagta aggaaattcc acccattgaa tttactcgac
     1081 ttcaattatt caatcaagat gtaaaaatca gagaaaaaaa ttcaccttta aaacaaagca
     1141 ttacttattc caacattatt gaactcacac ataaacaatc tgttttcagt atcgaatatg
     1201 ctgcacttga ttatgtaaat ccagaaaaaa ttcaatatgc ttttaaatta gaaaattttg
     1261 ataaagattg gaattatgtt caaaatcaac gcaaagcaac ttataccaat ttgccaaaag
     1321 gaacttatta ttttagagtg aaatccacca acaatgaggg agcaatggta aacaacgaaa
     1381 aaatgattac tattaaaatt ttaccttctt tttgggaaac ttcggttgcc tatgtgttat
     1441 atggaattgt ttttctactc ttactttaca ctgtttacta cctgaccagc atttacctaa
     1501 aacttaaaaa cgaagttatc attgaacaaa aagtaagtga aataaaatta cggtttttta
     1561 ctaacatctc tcatgaatta agaacgccac taactttaat tataggacct atagaaaata
     1621 tcttaaaaaa cgaaaaacta tctactacaa ctcacgagca acttaaaatt gtttataata
     1681 ataccaatag gatgatgcga ttaattaacc aaattctcga tttcagaaaa gttcaaaaca
     1741 ataaaatgcg attaaaagtt caacccatct atctggtacc atttttacaa aatatttgct
     1801 ctaactttaa agaagaagct caggaaaaaa atatggattt caagttcgtt aacaatgctg
     1861 gggatgtagt attgtggctg gatatggaaa aagtagatat aattttctac aatcttttat
     1921 cgaatgcttt taagttcact cccaacaaca aaaaaattga agttcgcatc gaacagagca
     1981 ataacaatga agacatccag ataaaagtca ttgatgaagg aataggaatc ccaaaagaga
     2041 aaaaaccatt tttatatgaa cgatttacct cccataatga aataaatacc ctaagcaacc
     2101 tatatggcag tggaattggt ttaaatctcg taaaagaatt tgtggatttg cataaaggaa
     2161 caattgaagt agaaagtgaa ccaggagaag gttctacctt tactgtcgtt tttcataaag
     2221 gaaaagatca ttttggcgat gaggtagata ttttagaaga aaaaagtaat gtgcttttac
     2281 ctgttgatga aagtttaatc gaccaaaatg atgctaaaat ttctattgaa gaattggaat
     2341 cttattatag tactgcaagc aaagaagcac cctttattct ggtaattgaa gacgacaagg
     2401 acatgcgtca tttcttaaaa atgattttaa gtaaaaatta tcgtgttgaa agtgctatag
     2461 atggtgttga tggatggggg aaaattcttt cattgtttcc cgatttggtt atatcagatt
     2521 taatgatgcc caataaagat ggtttggaac ttatacaact tatcaaagat aatgaaaaaa
     2581 caagccatat ccctgtaatt ttgctgactg aaaaaaatgc agttgaaagt aaattacaag
     2641 cctatcaata tggagctgat gattatatta ccaagccttt tagtcctatt ttactggaag
     2701 caagggtaga aaatcttctt caacaaagga aaaaacttca agaaaattat cgaaaacgat
     2761 tattcg
//
LOCUS       DAOTKS010000219         2757 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_29877_length_2757_cov_9.632494, whole genome shotgun sequence.
ACCESSION   DAOTKS010000219 DAOTKS010000000
VERSION     DAOTKS010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29877_length_2757_cov_9.632494"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1609)
                     /locus_tag="P5236_04965"
     CDS             complement(<1..1609)
                     /locus_tag="P5236_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63748.1"
                     /translation="MNPDTGSINQNFTEEESSFDFQKWLHLFIKNWYLFLIFIPFFLI
                     GGYLKNRSWMPLYQSVGTIIINENKYRSSSQGFMQGFGLEEAYQNVNNQVIILNSYDL
                     IGKVVDSLPFMKVDYLTIGRFKTRNLYTTTPIIIEADYVAPEAYELLFKISLHPNGTF
                     TISPHNNNSMPSSPINGHIGVPLQHNLFFMTVNSVSDNVKEFEMYFRFRTRETLIEDF
                     KSRLSSRYVIEGSSILEISLISETPDRDVDFINKLSEIYLEENLKKKNDVASKTIQFI
                     NEQLSEVSKSLSVSEDEMTRFRRDNQIIDISSHGSELMSKANSLDEKKAELKLKETYL
                     NYLTKYLTGNFEEGTVVAPSNLGLNEPLLMQLVQQINELYLQRSELTEKNLYYAKYTK
                     AIENVKTSIFELIKNMRASLEIEKNEVNRLLADVNSQIAELPKKELEIIGIERNYKVN
                     DNYYTFFLQKRAEAEIQKASNTPDNNILDQARTVSLINADVKSNTMFMFILLGILIPS
                     LIIVLKEVLNNTIRSIKDIEKRSVFPVI"
     gene            complement(1644..2390)
                     /locus_tag="P5236_04970"
     CDS             complement(1644..2390)
                     /locus_tag="P5236_04970"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HRU63749.1"
                     /translation="MKHFLHIIFTILTVLSFSSCYNYRSIGLLQENKSLPVYSKADYS
                     DYEICVNDEIIFRLITTDETISKLISSNQSGNSNQMISYRVYPDGTVDIPFLNRVQVK
                     GLTLNEAENLLKEEFREIIPDAEVKLSIANKSFTVIGEAGTGVFPVYKDKLTIYQALS
                     LSGDLKLSGDFRHVKILRPVEGKTEILEFDIRPASVIESKYYYVYPNDIIYVQRASSS
                     FFKMNNYSSFLGLISSSLTLLFSVLYYIKL"
     gene            complement(2417..>2757)
                     /locus_tag="P5236_04975"
     CDS             complement(2417..>2757)
                     /locus_tag="P5236_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63750.1"
                     /translation="SVIGGGATGRYPLNREKINIFEALAMAGDIDTYGDRSKIRIIRE
                     TNSGTQIKVFDVRSVDIINSEFYYVEPNDVIYIQDMNEQFFSVTSFASLLSTVFATFS
                     FGIFIYGLTR"
BASE COUNT          903 a          520 c          447 g          887 t
ORIGIN      
        1 caatcaccgg aaacactgat cgcttttcaa tatcttttat cgaacgaatg gtattgttca
       61 aaacttcttt tagcacaatt atcaaagaag ggatcaaaat accaagtaaa ataaacataa
      121 acatggtatt agacttgaca tctgcattaa tcagagatac ggtacgggct tgatccagaa
      181 tgttattgtc cggcgtattg gaagcctttt gaatttcggc ttcagctctt ttttgcagga
      241 aaaaagtata ataattgtcg ttgaccttgt aatttctttc aattccaata atttccaatt
      301 cttttttagg gagttcggct atttggctgt taacgtctgc aagaaggcga ttgacttcat
      361 ttttttcgat ttccaacgaa gccctcatat tttttatcaa ttcaaaaatt gaagttttca
      421 cattttctat ggctttggta tacttggcat aatacagatt tttttcggtt aattcgcttc
      481 gttgtaaata taattcgttg atctgttgaa ccaattgcat taacaaaggc tcatttagcc
      541 cgagatttga tggagcaaca accgttcctt cttcaaaatt acccgtgaga tatttggtca
      601 aataattcag ataagtttcc ttgagtttta gttcggcttt cttttcatcc aaagaattgg
      661 ccttgctcat caattcactc ccatgactcg aaatatcaat gatttgatta tcccttctga
      721 atcgtgtcat ttcatcttca gaaacagaca gggatttgga tacctccgat aattgttcat
      781 taataaattg aatggttttg gaggcaacat cgtttttctt tttcaaattt tcttcaaggt
      841 agatttcgct caatttgttg ataaagtcca catccctgtc tggagtttca ctgatcaatg
      901 aaatttccaa aatggaagaa ccttcaatga cgtatcttga acttaatcgc gatttaaaat
      961 cttcaatcaa tgtttctctg gtacgaaagc gaaaatacat ttcaaattcc ttaacattgt
     1021 cgctcacgct gtttacagtc ataaaaaata aattatgctg aagtggcacg cctatatgac
     1081 cattgattgg tgaacttggc atgctattgt tattatgtgg gctgatggta aaagtgccgt
     1141 tcggatgaag tgaaatttta aaaagcagtt cgtaagcttc cggagccaca taatcggctt
     1201 caataataat aggagttgta gtataaagat tacgggtttt gaaacgacca atcgtcaaat
     1261 agtccacttt cataaaaggt aaactatcta caactttgcc aatcaaatcg tatgaattga
     1321 gaattatcac ctgattattg acattctggt aagcttcttc caacccaaat ccctgcatga
     1381 aaccttgtga agaggatcgg tatttgtttt cattaataat aattgttcca acactctgat
     1441 atagaggcat ccaacttctg ttttttaaat atcctccaat taaaaaaaac ggaataaata
     1501 ttaaaaacaa ataccaattt ttgataaata aatgaagcca cttctgaaaa tcaaaggaag
     1561 attcttcttc ggtaaaattc tggttaatgc tgcctgtatc cggattcata tcttagaata
     1621 aaagataggt ttatgctgtg aatttacaat ttgatataat ataaaacgct gaacaataaa
     1681 gttaaagaag acgaaatcag tccaagaaaa gaagaataat tgttcatttt aaaaaagctc
     1741 gacgatgccc gctgcacata aataatatcg ttggggtaaa cataatagta tttcgattca
     1801 attacacttg ccggacgaat atcaaactcc aatatctcgg tttttccctc cactggtcgt
     1861 aaaattttca catgccggaa atcaccggaa agtttcaaat cacctgacag ggacaaagcc
     1921 tgataaatag taagtttatc cttatataca gggaaaacgc cagtccctgc ttctccaatt
     1981 actgtaaatg atttattggc aatactcagt ttcacttcag catcaggaat aatttccctg
     2041 aattcttcct tcaacaaatt ctcagcttca tttagggtca atcctttcac ctgaacgcga
     2101 tttaaaaagg gaatgtccac agtaccatcc ggataaacgc ggtaagaaat catctgattg
     2161 ctattgcctg actgattgga agaaatcaac tttgaaatgg tttcatccgt agtgatcaaa
     2221 cgaaaaataa tttcgtcgtt aacacaaatt tcataatccg aataatctgc cttagaataa
     2281 acgggtagcg atttgttttc ctgcagtaac ccaatgcttc tgtaattgta acaggaagaa
     2341 aaggacaaaa cagtcaaaat tgtaaaaatt atatgaagaa aatgtttcat aattttttca
     2401 tctctcttca aaccgattaa cgggtcaaac cataaataaa aattccaaat gaaaaagtag
     2461 caaaaaccgt cgacaaaagt gaagcaaagg acgttacact gaaaaactgt tcgttcatgt
     2521 cctgaatgta aatcacatca ttgggctcaa cgtaataaaa ttcggaattg ataatatcca
     2581 cacttcgcac atcaaaaacc tttatctgag ttccactgtt tgtttctctg ataatcctta
     2641 ttttacttct atcgccataa gtatcaatgt cgccggccat tgccagcgct tcaaaaatat
     2701 ttattttttc ccgatttaaa ggatacctgc cggttgcacc tccaccaata acactga
//
LOCUS       DAOTKS010000220         2748 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_30007_length_2748_cov_4.411808, whole genome shotgun sequence.
ACCESSION   DAOTKS010000220 DAOTKS010000000
VERSION     DAOTKS010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30007_length_2748_cov_4.411808"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="P5236_04980"
     CDS             <1..418
                     /locus_tag="P5236_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63751.1"
                     /translation="FFSAKTFLQNRLGLNDSLQQNFYKYPAICPVNKNIVGEPSAQPQ
                     NIRIVKDGDAAYLLWDEVEEEGGCQVAYYVVYAFKGKKVGDLNDPANIIARTTDNCLD
                     LRALNVHLKGHYTFVVTAINRFKQESIPTVAVTRKL"
     gene            complement(684..1340)
                     /locus_tag="P5236_04985"
     CDS             complement(684..1340)
                     /locus_tag="P5236_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HRU63752.1"
                     /translation="MKIAISSLVHSICPDLVLGIIEGKVKNSSTSALLWQEVEKEMGE
                     ILSKYSLKQISKRPAILATREAYKKFGKDPSRYRPSAEALCRRICKGNSIYQINTLVD
                     IINLVSIHSGFSIGGFDADKIQGNLLLDVGNENDEFEAIGRGILNVAGLPLFRDEVGG
                     IGTPTSDNERTKITMETNHLLMIINAYGGKTGLEKTIDYAVDLLKKYAEMTAYNIFLS
                     "
     gene            complement(1362..2150)
                     /gene="pgeF"
                     /locus_tag="P5236_04990"
     CDS             complement(1362..2150)
                     /gene="pgeF"
                     /locus_tag="P5236_04990"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HRU63753.1"
                     /translation="MENLLTDSPLFDQFPDIQYFFTTRKGGYSYGNYATFNLSPFVGD
                     KPQHVAQNLNKLAEILSLPTDKIFFPHQNHGKFIHKIDETFLQLTPEEQKNHLANVDG
                     LYTNLKNVCISVTTADCVPLLFYDPVKKAVAVAHAGWRGTVQQIAAKMVATLVRKYGC
                     SPKNIRVVMGPAISEQKYEVGKEILLTFIIAGFETSEIFIQQENKIFLNLWKANEMIL
                     LGSGVLKSNIEIKELCTFTNSSDFFSARRDGAYCGRMLNGIILR"
     gene            complement(2197..>2748)
                     /locus_tag="P5236_04995"
     CDS             complement(2197..>2748)
                     /locus_tag="P5236_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HRU63754.1"
                     /translation="ELTLHVGAGTFQPVKTQNVLEHEMHTEVFMVSKNTIEQLLDKLG
                     NIIAVGTTSARTLESLYYIGLLLSQDSSLKEKPTSFFLSQWLPYTMKNEISTKTALTL
                     ILEYLEKNKLSHLYAQTQIMIQPAYTFHLINGLITNFHQPKSTLLLLISAFVGDDWRK
                     IYHYALKHNFRFLSYGDSSLLLK"
BASE COUNT          914 a          465 c          449 g          920 t
ORIGIN      
        1 gttttttagt gccaaaactt ttttacaaaa caggctagga ttgaatgata gtttacagca
       61 aaatttttat aagtatcctg ccatttgtcc cgtgaataaa aatattgtag gggaaccttc
      121 cgctcaacca caaaatataa gaattgtaaa agatggagat gctgcttatt tattgtggga
      181 cgaagtagaa gaagaaggcg gatgtcaagt agcctattat gtggtgtatg cttttaaagg
      241 aaagaaagtt ggtgatttga acgatcctgc taatattatt gctcgaacta cagataattg
      301 tcttgattta cgagctttaa atgttcatct gaaaggacat tatacttttg ttgtaactgc
      361 tattaatcgt tttaaacagg aaagtattcc aacagtggca gtgaccagga aattgtagaa
      421 ttatttatga ttatccgcgt ttaacacaac aattggttta ttttcaggta cttttatttc
      481 taaaccaaga attgcattga gaaaataagt atttagaaag tttaataaaa aaggctataa
      541 agtattaaaa gtcaatcaag tattttgttt tggtatctga taaattttat atcaaattga
      601 tatatttatc gatatatcat caaagaacaa aggtgcgtag caccgatatt tttgtaccca
      661 ctaatacaaa atgttattca gttttaggat aaaaaaatat tatatgccgt catttcagca
      721 tattttttga gtaagtccac tgcataatct atagtttttt caagtccagt tttacctcca
      781 taagcgttga taatcatcaa taaatgatta gtttccatag ttatttttgt tcgttcatta
      841 tcgctggtag gtgttccaat accacctact tcgtctcgaa aaagcggaag accggcaaca
      901 tttaaaattc ctctgccaat agcttcaaac tcatcatttt catttcccac atcgagtaaa
      961 agatttcctt gaattttgtc tgcatcaaaa ccaccaattg aaaaaccact gtgaatagaa
     1021 acaagattga taatatcgac cagtgtattg atttgataaa tggaattccc tttacagatt
     1081 cgtctgcata aagcctctgc tgaaggacga taacgtgaag gatctttacc aaatttttta
     1141 taagcttctc ttgtggctaa aatagcaggg cgtttactga tttgtttcaa tgagtatttt
     1201 gaaagaatct ctcccatttc tttttccacc tcttgccata acaacgcaga agttgatgaa
     1261 tttttgactt ttccttcaat aatacccaaa actaaatcag gacaaatact gtgaaccagt
     1321 gaagagattg ctattttcaa aacaataaat taaaaatgtt tttatcgtaa aataattccg
     1381 ttcaacattc ttccacagta cgcgccatct cgacgggctg agaagaaatc gcttgaattt
     1441 gtgaatgtac ataattcctt gatttcgata ttagatttta aaacgccgct ccccaacaaa
     1501 atcatttcgt ttgctttcca gagatttaaa aaaatcttat tttcttgttg aatgaaaatt
     1561 tctgatgttt caaagccagc aattataaaa gttagtaaaa tttcttttcc tacttcatat
     1621 ttttgttccg aaatggcagg acccataacc acacgaatat ttttaggaga acaaccgtat
     1681 ttccgtacaa gtgtagctac catttttgct gcaatttgct gaacagttcc tctccaacca
     1741 gcatgagcca cagcaactgc ctttttaaca ggatcataaa acaataatgg cacacaatcg
     1801 gcagtagtga cactaataca aacatttttc aaatttgtgt ataagccatc tacattggca
     1861 aggtgatttt tctgttcttc gggggtcaac tgcaaaaaag tttcatcaat tttatggatg
     1921 aattttccat gattttggtg agggaaaaaa attttatctg ttggcaatga aagaatttcc
     1981 gctaatttgt tgagattctg agcaacatgt tgaggtttgt caccaacgaa aggactcaaa
     2041 ttaaaagtag cataattccc ataactgtaa cctccttttc tggtagtgaa aaaatattga
     2101 atatcaggga attgatcaaa caaaggggaa tcagtcaata aattttccat agatttttgt
     2161 tttaactgtt ttagctttta gaactttctt gaaaaattat ttcagcagta aggaactgtc
     2221 accataactt aaaaaccgaa agttatgttt cagagcatag tgataaattt tccgccaatc
     2281 atcaccaaca aaagctgaaa taagtagtaa taaagtactt ttcggctgat gaaaattagt
     2341 aatcaatcca tttatcagat gaaatgtata agccggctgg atcatgattt gtgtttgagc
     2401 ataaagatgg ctcaatttat ttttttccaa atattccaga ataagagtta atgctgtttt
     2461 tgttgatatt tcatttttca tcgtatatgg taaccattga gaaagaaaaa atgaagtcgg
     2521 cttttctttc agcgacgaat cctgacttaa taaaagccct atataataaa gactttccaa
     2581 cgttcgtgct gaagtagttc caacagcaat aatatttccc aacttatcaa gtaattgttc
     2641 aatagtattt ttcgaaacca tgaacacttc ggtatgcatc tcatgctcta aaacattctg
     2701 tgttttcact ggctgaaaag taccagcacc aacgtgcaga gtcaattc
//
LOCUS       DAOTKS010000221         2719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_30449_length_2719_cov_3.494369, whole genome shotgun sequence.
ACCESSION   DAOTKS010000221 DAOTKS010000000
VERSION     DAOTKS010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30449_length_2719_cov_3.494369"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1232
                     /locus_tag="P5236_05000"
     CDS             <1..1232
                     /locus_tag="P5236_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HRU63755.1"
                     /translation="TWWKKVKNKSLRSFLSWVDAIIFALVAVYFINLFLFQNYQIPSS
                     SLEKTLLVGDFLFVSKVNYGPREPFTPLSFPLVQNTFPIINTKSYLEKPQWDYKRLKG
                     LDTIARNDIVVFNFPTGDTVALKQQNPDYYQLCYYYGREVVHSRKDIFGEIVYRPVDR
                     RENYVKRCVAVAGDWVEIRNNQVYVNGKKEEKFPGIQFNYLVQTDGRRLSDELLSKLH
                     ISKDDRLLLNTMQNPSEIISLGLDPELPIYHFPLTEEAYNKLRNVSGVTKIIVEPEQY
                     GGEVFPLGGGKNWTRDNYGPIYIPRKGAKLVLNAYNYPIYERVIRVYEKNKLEMKEGK
                     FYINDKPATTYQFKMNYYWMMGDNRHNSADSRYWGFVPEDHIVGQPVFVWLSLDKDKS
                     WFNGKVRWKRFFKNATR"
     gene            1315..1926
                     /locus_tag="P5236_05005"
     CDS             1315..1926
                     /locus_tag="P5236_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HRU63756.1"
                     /translation="MPAILSLAYLAPVEYYKVLANSSEIFIEKEENYIKQTYRNRCKI
                     LTANGVMDLIIPVEKSEGNKPSIKDVKISVHSDWQKQHWRAIVSAYSSSPFFEYYEDD
                     LYPFFKKKWLFLWDFNLEIQNKIIELLDLETQINFTEKFEPFPADKRIDLREEFHPKR
                     QSKIEYSPYYQVFRGKFGFVKGLSVIDLLFNMGNESQLILSEK"
     gene            1990..>2719
                     /locus_tag="P5236_05010"
     CDS             1990..>2719
                     /locus_tag="P5236_05010"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HRU63757.1"
                     /translation="MEIDWNNLGFGYIKTDYNLRCTYKDGKWGDFEVHDDEYLTLHMA
                     STCLHYGQEVFEGLKAFRGKDGKIRIFRMDENAKRMQSSCAGIVMAQLPVEKFEEAVL
                     KVVKLNERFVPPYESGASLYIRPFMIGTSAQVGVKPAREYQFIIFVTPVGAYFKGGFK
                     ATPFVIFREYDRAAPHGTGIYKVGGNYAASLVPDEKAHAMGYSAVFYLDPKEKKYIDE
                     CGAANFFGIKNNTYITPKSTSILSS"
BASE COUNT          923 a          410 c          529 g          857 t
ORIGIN      
        1 ggacctggtg gaagaaagta aaaaataaaa gtctacgttc ttttttaagc tgggtagatg
       61 ctatcatatt tgcattagta gcagtatatt ttatcaattt atttttattc caaaattatc
      121 aaatccccag ttcttctctc gaaaaaacat tattggtggg tgattttctt tttgttagca
      181 aagtaaatta tggaccacgt gaaccattta cccctttatc atttcctttg gtacaaaaca
      241 ctttcccaat cattaacaca aaatcatatc tcgaaaagcc tcaatgggat tataaacgtt
      301 taaaaggact cgacaccatt gcccgaaatg atattgtggt atttaatttc ccaactggcg
      361 ataccgttgc cttaaagcaa caaaatcctg attattatca gctttgttat tattatggca
      421 gagaggtagt ccacagtcgg aaagatattt ttggagagat cgtatatcgt cccgtcgatc
      481 ggagagaaaa ttatgtaaaa cgttgcgtgg cagtggctgg tgactgggtg gaaatcagga
      541 ataatcaagt ttatgttaat ggtaaaaaag aggaaaaatt ccccggtatc caatttaatt
      601 atctcgtgca aactgatggg cgaaggcttt cagacgaatt attaagtaaa ttgcatataa
      661 gtaaagatga tcgattattg ttaaatacca tgcaaaatcc aagtgaaatt atttcattgg
      721 gtttagatcc tgagcttccc atttatcatt ttcctttaac agaagaagcc tataacaagt
      781 tgaggaatgt ttcaggtgtt acaaaaatca ttgttgaacc cgaacaatat ggtggagaag
      841 tttttccatt aggtggcggt aaaaattgga cacgtgataa ttatggacca atttatatcc
      901 ctcgaaaagg agccaagcta gtattaaatg catacaatta tcccatttat gagcgagtaa
      961 ttcgggtata tgaaaaaaat aagcttgaaa tgaaggaagg caaattttat atcaatgata
     1021 agcctgccac aacttaccaa ttcaaaatga attattactg gatgatgggt gacaacagac
     1081 ataattcggc tgattcacgt tattggggat ttgtccccga agatcacata gtgggtcaac
     1141 ctgtctttgt ttggttgtcg ttggataaag acaagtcgtg gttcaatgga aaagttagat
     1201 ggaagaggtt ttttaaaaat gcaacacgat aaagatactt ttgatttttc gttaaaaaaa
     1261 attcagtata agaatcaagc aaaaatcctt agtcaatgga agagtgagaa ttcaatgcct
     1321 gccattttat cattagcata tttagcacct gttgaatatt acaaagtgct ggcaaatagt
     1381 tcagaaattt ttattgaaaa agaggaaaat tatatcaaac aaacctatcg gaatcgttgt
     1441 aaaattctta ctgctaatgg agtgatggac ttgattattc ctgtggagaa atcggaagga
     1501 aacaagccgt ctatcaaaga tgttaaaatt tctgttcatt ctgattggca aaaacaacat
     1561 tggcgagcga ttgtttctgc ctattcttca agtccttttt ttgaatatta tgaagatgat
     1621 ttatatcctt tttttaaaaa aaaatggtta tttttgtggg atttcaatct tgaaattcaa
     1681 aataaaatta ttgaacttct tgatttggaa actcaaataa actttacgga aaaatttgaa
     1741 ccatttcccg ccgacaaaag gattgatttg agagaagaat ttcatcccaa gagacaatcg
     1801 aaaattgaat attctcccta ttatcaagta ttcaggggaa aatttggatt tgttaagggt
     1861 ttgagcgtga ttgatttgct ctttaacatg ggcaatgaaa gtcaattgat tttatctgag
     1921 aagtaatttt gatgaagaat actaaacaag ttattataac taacaacatt ataaaatatt
     1981 tcaaacaaaa tggaaattga ttggaacaat ttaggattcg gctatattaa aaccgattac
     2041 aatcttcgat gcacctataa agatggcaaa tggggtgatt ttgaagtaca tgatgatgaa
     2101 tatttaacat tgcacatggc atctacttgt cttcattatg gacaagaagt ttttgaaggg
     2161 ttaaaagctt tccgagggaa agatggaaaa attcgtattt tccgcatgga cgaaaatgcc
     2221 aaacgaatgc aatcgagttg tgcaggtatc gtaatggcac aattaccggt tgaaaaattt
     2281 gaagaagcag tattgaaagt tgtgaaattg aatgaacgtt ttgtgcctcc ttatgaatca
     2341 ggagcctcgt tatatatacg tccgtttatg attggaacaa gtgcccaagt tggtgtgaaa
     2401 cctgccagag aatatcaatt catcattttt gtgacgccag taggagccta ttttaaaggt
     2461 ggttttaaag ctactccatt tgttattttt cgtgaatatg atagagcagc tccacatgga
     2521 acaggaattt ataaggtggg cggcaattat gctgcgagtt tggtgcccga cgaaaaagct
     2581 catgcaatgg gttattcagc ggtgttttat ttagatccca aagaaaaaaa atatatcgac
     2641 gaatgcggag cagcaaattt ctttggaata aaaaacaata cttacataac tccaaaatct
     2701 acttcgattc tgtcttcta
//
LOCUS       DAOTKS010000222         2716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_30494_length_2716_cov_2.691845, whole genome shotgun sequence.
ACCESSION   DAOTKS010000222 DAOTKS010000000
VERSION     DAOTKS010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30494_length_2716_cov_2.691845"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="P5236_05015"
     CDS             complement(<1..931)
                     /locus_tag="P5236_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRU63758.1"
                     /translation="MKKLIITVIFLYACIIAFSQTFLLKGVVTSTDGETLIGVNVIKS
                     GTTIGTITDVDGKFEIQVKAGDKLKFSYVGYKAQEIIVKPNQQYINIQLAPDQFTNLD
                     EIVVIGYGKAKKLTMTGAVSAIPMREIRLVPTSSVQNALSGKLPGFFSVQRSGQPGKD
                     ASDFFIRGVSSLNDAGNQPLIIVDDVQYSYDQLQQININEIESISILKDASTTAIYGI
                     KGANGVLVVKTRRGEQGAPQINVRMESGIQTPVRKPKFLNSYETAKLVNEAYQNDGLI
                     SQIPFSEDDLEAFRTGSDPYGHPDVNWYNEIFKS"
     gene            complement(938..2716)
                     /locus_tag="P5236_05020"
     CDS             complement(938..2716)
                     /locus_tag="P5236_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRU63759.1"
                     /translation="MKSKQNIWMIPIVGIVLLFFVACTDSYESFPVEQFTKDYVFSKT
                     DSMGVQARNFLNSLYGMLDNGHNGVGGDYLDAASDDAISIKQNEPDVYKLAVGQYSAN
                     NRISSTMGWGKYYEAIRKANIIIKNIDAVPFKLTFVNINGEVKPLNYTIKAEARFLRA
                     YFYFELVKRYGGVPLLGDSIYNLEDNMEIPRNSFADCIDYMVRELDTIKYDLRSVTMP
                     DPSVYAHAATIEAAMALKSRILLYAASPLFNEKPITTDNNAQKLLIGYESYDIERWKK
                     AADAAKYFIDTFGPTGKKTVDLAKNQMGVFLSFYSTTANPELIFFRQGGMDKSVETNN
                     GPLGFSGNSLGNGRTNPTQNLVDAFLMKDGKPRGESTLYPYDPQKPYDNRDPRLGLTI
                     LYNGANWLGRVLETYRGGANNPTGTGEYTKTSYYMRKFMGNFANATEYSNNYHHWVIL
                     RYTEILLNYAEALNEYLPAPSQDVYNAIISLRKRAGIPAGSDKLYGLKANMTKEEMRK
                     VIQNERRIEMAFEEQRYWDIRRWRIAEDIFKTPLKGMNIIKTLSGLNYSEVDVLTANF
                     EEKQYLYPIPYSEVIKNRNMVQNPKW"
BASE COUNT          840 a          535 c          391 g          950 t
ORIGIN      
        1 ttgatttgaa aatttcatta taccaattaa cgtcaggatg accgtaagga tcagaaccag
       61 ttctaaacgc ttccaaatca tcttctgaaa atggaatttg agaaattaag ccatcatttt
      121 ggtacgcttc atttaccaat ttagcagttt cgtatgaatt aagaaatttt ggttttctaa
      181 ctggtgtttg aatcccactt tccattctta cgttaatttg aggtgcccct tgttctcccc
      241 ttcgtgtttt taccactaat accccatttg cacccttgat gccatagata gcagtagtag
      301 aagcgtcttt caatatggaa atactttcta tttcatttat attgatctgt tgtaactgat
      361 cgtatgagta ttgtacatca tctacgataa tcaaaggttg attaccagca tcattcaaag
      421 agctaactcc ccgaataaag aagtctgaag catctttacc aggttgacca gagcgttgta
      481 ctgaaaaaaa tcctggcaat ttgcctgata gagcattttg cacactgctt gttgggacta
      541 aacgtatttc acgcattgga attgcactaa ctgcacctgt catcgttaac ttcttagcct
      601 ttccataacc tattacaaca atttcatcta agtttgtaaa ttgatcggga gctaattgaa
      661 tatttatata ctgttgatta ggctttacaa ttatttcttg agctttgtaa ccaacataag
      721 aaaattttaa cttatcacct gccttaactt ggatttcaaa ttttccatca acatctgtaa
      781 tggtaccaat agttgtacca ctttttataa cattaacccc aataagtgtt tcaccatcag
      841 ttgaagttac aacgcctttt agtaaaaaag tttgactaaa agctattata caggcataca
      901 gaaatatcac tgttattatt aattttttca taagaaatta ccatttagga ttttgaacca
      961 tgtttctatt tttaataact tctgaataag ggattgggta taaatattgt ttttcttcaa
     1021 aatttgcagt cagcacatca acctctgaat aatttaagcc cgaaagagtt tttataatat
     1081 tcatcccttt taaaggcgtt ttgaaaatat cttctgcaat tcgccatctt cttatatccc
     1141 aataacgttg ttcttcgaaa gccatttcaa tccgacgttc gttttgaatt actttacgca
     1201 tttcttcttt tgtcatattc gcttttaatc catataattt atcgctgcct gctggaatcc
     1261 cagccctttt ccgtaacgat ataatagcat tatacacatc ttgagatgga gcaggtaaat
     1321 attcatttaa cgcctcagcg taatttaaaa gaatttctgt atatcgaaga ataacccaat
     1381 gatgataatt attactatat tcagttgcat tcgcaaaatt tcccataaat ttacgcatat
     1441 agtaactcgt ttttgtatat tcacccgttc ccgtaggatt atttgctcct cctcgataag
     1501 tttctaaaac tctgcctaac caatttgctc cattatatag tatggttaat cctaaacgag
     1561 gatctctgtt atcataaggt ttttgaggat cataaggata taaagtactt tcccctctcg
     1621 gcttgccatc tttcattaaa aatgcatcca caaggttttg agtaggattg gtacgcccat
     1681 tcccaaggga atttccacta aaaccaagtg gcccattatt agtttctaca cttttatcca
     1741 ttcctccctg acgaaaaaaa ataagttctg gatttgcagt agtactgtaa aaacttagga
     1801 aaacacccat ttgatttttt gctaaatcaa ctgttttttt accggttgga ccaaaagtat
     1861 caataaaata tttggctgca tcagcagctt ttttccatcg ttcaatgtca tagctttcat
     1921 aaccaatcaa tagcttttga gcattattat ctgtagttat tggtttttca ttaaacaatg
     1981 gacttgcagc ataaagtaaa attcgagatt tcaatgccat tgcagcctca attgtagccg
     2041 catgagcgta gacagatgga tcaggcattg ttacactcct caagtcatat tttattgtat
     2101 ccagttccct taccatataa tcaatacaat ctgcaaatga gtttctcggg atttccatat
     2161 tatcctctaa attatagata gaatctccta acaatggaac acctccataa cgtttcacta
     2221 attcaaaata aaaataggct cttaagaaac gtgcttctgc ttttattgta taattcaaag
     2281 gtttaacttc tccattaata tttacaaaag tcagtttaaa agggactgca tcaatattct
     2341 tgatgataat gttagcttta cgaatggctt cataatattt tccccaaccc atagttgaac
     2401 taatgcggtt attagcacta tattgaccca cagctagttt ataaacatca ggttcgtttt
     2461 gtttgatgga aattgcatca tcagaagctg catctaaata atcccctcca accccattat
     2521 gaccattatc aagcattcca tacaaagaat ttagaaaatt tcttgcttga actcccattg
     2581 aatctgtttt agaaaaaacg taatcttttg tgaactgctc aacgggaaag ctttcataac
     2641 tatcagtaca agctacaaaa aatagcagaa ctattcccac tataggaatc atccatatat
     2701 tttgtttcga tttcat
//
LOCUS       DAOTKS010000223         2699 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_30706_length_2699_cov_3.005295, whole genome shotgun sequence.
ACCESSION   DAOTKS010000223 DAOTKS010000000
VERSION     DAOTKS010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30706_length_2699_cov_3.005295"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="P5236_05025"
     CDS             complement(<1..255)
                     /locus_tag="P5236_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63760.1"
                     /translation="MIATETKIVNSEIIKEIEEKIENVYSYLNKQKEHVNSLNVFPVP
                     DGDTGLNMVLTIQGAIAALRSNNGYSSMTPGKFLKNFAENM"
     gene            599..2509
                     /gene="dnaK"
                     /locus_tag="P5236_05030"
     CDS             599..2509
                     /gene="dnaK"
                     /locus_tag="P5236_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRU63761.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEANQPVVIPNNEGKRTTPSVVAFLE
                     NGERKVGDPAKRQAIINPTRTVYSIKRFMGRTYDEVTAEAARVPFKVVKGENNLPKVD
                     IDGHLYSPQEISAIILQKMKQTAEDYLGTQVTDAIITVPAYFNDAQRKATKEAGEIAG
                     LNVRRIINEPTASALAYGLDKTNREMKIVVFDCGGGTHDVSILELGDGVFEVKATDGD
                     THLGGDDFDQRIIEWLVQEFKNENSGVDLSKDPMALQRLKEAAEKAKIELSTATSTEI
                     NLPYIMPVDGIPRHLVRTLTRAKFEQLIDDLIQRTIEPCRSALQKAGLTVNDINEVIL
                     VGGSTRIPAIQQAVEKFFGKAPSKGVNPDEVVAIGAAIQGGVLAGEVKDILLLDVTPL
                     SLGIETLGGVMTKLIEANTTIPTRKSEIFTTAADNQPSVEIHVLQGERPLASQNKSLG
                     RFHLDGIMPAPRGVPQIEVTFDIDANGILHVSAKDKATGKEQSIRIEASSGLTEAEIK
                     RMKEEATANAEADAREKERIEKLNHADSLIFQTEKQLNEFGDKIPADKRAPIDAALNR
                     LKEAYKSQNLEEIDKATHELNTAFQAASQEMYNAQQQASQGAGYTGEAGPQGQGGSNQ
                     EGDVTDVDFEEV"
BASE COUNT          927 a          432 c          511 g          829 t
ORIGIN      
        1 catattttcg gcaaaatttt tcaaaaattt gcctggtgtc atcgaactat aaccattatt
       61 acttcttaaa gctgcaatag ctccttgaat agttaatacc atgttgagcc ccgtatcgcc
      121 atctggcaca ggaaaaacat tcaaactatt tacatgctct ttttgcttat ttaaataaga
      181 ataaacattt tcaatttttt cttcaatttc ttttatgatt tcagaattaa caatttttgt
      241 ttctgtcgca atcatagcaa ttaaattaaa taggtattaa tttattttta tttttatttt
      301 tatttttatt tgcagctaca aagttataat tttattttaa aataaaaatt aaccttttga
      361 tatgggtagc tgaattattt ttactcaatt atcaaaataa gtgctaaaat atttcttttc
      421 tggcacaata gcatattttc tgcatttttg tcagtatttt tattcacatt tcttcgtttt
      481 ttaagtttct cccttaaaaa atattattat taatgacaat taaccagatg gcacaacctt
      541 tgcaaagtga agaataaaaa agtaaaaaat attaaataat catcataaaa ataaagttat
      601 gggaaaaatt attgggatag acttaggaac caccaactct tgtgtggctg taatggaagc
      661 aaatcaacca gttgttatac cgaataatga agggaaacga accactccat cggtagttgc
      721 tttcttggaa aatggagaac gaaaagtggg tgatccagca aaacgtcaag ctattattaa
      781 tccaacaaga acagtatatt ccatcaagcg ttttatgggg cgtacttacg atgaagtaac
      841 agcggaggca gcacgcgtac catttaaagt agtaaaagga gaaaacaatt taccaaaagt
      901 agatattgat ggacatttat attctccaca agaaatttca gctattattc ttcaaaaaat
      961 gaagcaaact gctgaagatt acttaggtac gcaagtaact gatgctatta ttaccgtacc
     1021 tgcctatttt aatgatgcac aacgtaaagc aacgaaagag gcaggagaaa ttgcaggatt
     1081 gaatgtacgt cgtatcatca atgagcctac cgcttcggct ttggcttatg gtttggataa
     1141 gacgaatcgt gaaatgaaaa ttgttgtatt cgattgcgga ggaggtactc atgatgtatc
     1201 tattctggaa ttaggtgatg gtgtttttga agtaaaagcc acagatggtg atacacattt
     1261 gggaggagat gatttcgatc aacgtattat tgaatggttg gttcaggaat ttaaaaatga
     1321 aaacagcggt gttgatttaa gcaaagatcc aatggcttta cagcgattaa aagaagctgc
     1381 agaaaaagca aagattgaat tgtctaccgc tacttctaca gaaatcaatt taccttatat
     1441 tatgcctgta gatggaattc ctcgtcacct tgtgcgtact ttgacacgtg caaaatttga
     1501 acagttaatt gatgatctca ttcaaagaac cattgaacct tgccgaagtg cacttcagaa
     1561 agcaggtttg acagtgaatg acattaacga agttattctt gtcggtggtt caacacgtat
     1621 tccagctatt cagcaagcag tagaaaaatt ctttggcaaa gctccttcaa aaggcgtcaa
     1681 tccagatgaa gtagttgcta ttggtgcagc tattcaaggt ggtgtgttgg caggtgaagt
     1741 aaaagatata ttgttactcg atgttacgcc tctttcatta ggtattgaaa cattgggtgg
     1801 tgttatgaca aaattaatcg aagccaatac aactattcct acacgtaaat cagaaatctt
     1861 tactactgca gccgataatc aaccttctgt ggagattcat gtactgcaag gagaacgacc
     1921 attagcttca caaaataaat cattaggacg tttccatttg gatggtatta tgccggctcc
     1981 acgtggagtt cctcaaattg aagtaacatt cgacattgat gctaatggaa ttcttcatgt
     2041 atcggctaaa gacaaagcaa caggtaaaga acaaagcatt cgaatagaag catcttcagg
     2101 tttaacggag gctgaaatca agcgaatgaa agaagaggct actgctaacg ccgaagcaga
     2161 tgccagagaa aaagaacgaa ttgagaagtt gaatcatgct gacagtttaa tcttccagac
     2221 tgaaaagcag ttgaatgaat ttggagataa aattccagca gataaaagag ctccgattga
     2281 cgctgctttg aaccgactga aagaagctta taagtcgcag aacttagaag aaattgataa
     2341 agctactcac gagttaaata ctgcttttca agcagccagc caggaaatgt acaatgcaca
     2401 acagcaggca agtcaaggag caggttatac aggtgaagcc ggtccacagg gtcagggagg
     2461 cagtaatcaa gaaggcgatg taacagatgt ggactttgaa gaagtttaaa tagttcatac
     2521 tttcaaatga aaatctctgt aaacgaattg tttgcagaga tttttttgtg ttcatttttt
     2581 atctgagggt tattaaaata aactaaaaat cgtttttttt atcagactat attttatcac
     2641 aattttggaa aaaaaattgc ttatcgtcag aattacaaat tttcaaaaaa ttattttac
//
LOCUS       DAOTKS010000224         2686 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_30885_length_2686_cov_4.952870, whole genome shotgun sequence.
ACCESSION   DAOTKS010000224 DAOTKS010000000
VERSION     DAOTKS010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30885_length_2686_cov_4.952870"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..2102
                     /gene="thrS"
                     /locus_tag="P5236_05035"
     CDS             159..2102
                     /gene="thrS"
                     /locus_tag="P5236_05035"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRU63762.1"
                     /translation="MIKITFPEGLVKEFPKGITGQEIAENISPKLAQEALAIGVNDEI
                     WDLTRPIENDATVKIFTWDDEEGKHAFWHSSAHLMAEALEELYPGIKFGIGPAIENGF
                     YYDVDAGNTIIKETDLPQIEEKMKELAARKEPIIRKNISKADALKIFEEKGDEYKIEL
                     IQDLTDGTITLYSQGNFTDLCRGPHLPDTGKIKAIKLLSVAGAYWRGDEKRKQLTRIY
                     GITFPTKKMLDDYLVLLEEAKKRDHRKIGKELELFMFSDMVGKGLPIWLPRGTDLRLR
                     LEEILKKIQRRFDYQQVITPHIGNKQLYITSGHYAKYGKDSFQPIHTPEEGEEYLLKP
                     MNCPHHCEIYKFKPRSYKDLPLRIAEFGTVYRYEQSGELHGLTRVRSFTQDDAHIFCR
                     PEQVKDEFLKVMDIIFIIFKALDFNNFEAQISLRDPNNKEKYIGTDENWDKAEKAIVE
                     ACEEKGLKARVMLGEAAFYGPKLDFMVKDAIGRRWQLGTIQVDYNLPERFELEYTGED
                     NQKHRPVMIHRAPFGSMERFVALLIEHTAGKFPLWLAPDQVVILPISEKYNQYAFEVE
                     KILKEKSIRAIVDDRNEKIGRKIRDNELKRIPYLLIVGEKELTGREVSVRRQGEGDKG
                     TMKIEDFAQLILNEINILLKQFD"
BASE COUNT          948 a          401 c          483 g          854 t
ORIGIN      
        1 aaataaaaac aaaagctacc ccgacgcaag catcggggaa ttcttacgat taaaaactca
       61 gcacacctaa cattccaact tactttcctt atttcctttt gaagaaaaaa aactatcttt
      121 gcaccattaa attaatactt aatggcaaaa tttttaaaat gattaaaata acttttcctg
      181 aaggtttagt aaaagaattt cctaaaggta ttactgggca agaaattgca gaaaatatta
      241 gtccaaaatt agctcaagaa gcattggcta ttggtgtaaa tgatgaaata tgggatttaa
      301 ctcgacctat tgaaaatgat gcaacggtaa aaatatttac ctgggacgat gaagaaggta
      361 aacacgcttt ctggcattct tctgctcatt taatggctga agctcttgaa gaactgtatc
      421 caggtatcaa atttggcatt ggtccagcca ttgaaaatgg tttttattat gatgtggatg
      481 ccggcaacac gattattaag gaaaccgatc ttccacaaat tgaagaaaaa atgaaggaac
      541 ttgctgctcg taaggagcct attattcgaa agaatataag taaagcagat gccttaaaaa
      601 tatttgaaga aaaaggcgac gaatataaaa tagaattaat acaggatctg accgatggaa
      661 ctattacttt gtattcacaa ggaaatttta ctgatctttg ccgaggtcct catttacctg
      721 atacaggaaa aattaaagcc ataaaattat tgagtgtagc tggggcttat tggcgaggcg
      781 atgaaaaacg aaaacaactt acacgaattt atggaattac tttcccaaca aagaaaatgt
      841 tggatgacta tctggtactt cttgaagaag ctaaaaaaag agatcatcga aaaataggaa
      901 aagaacttga actatttatg ttctccgata tggtagggaa aggattgcct atctggttgc
      961 ctcgtggaac agacttacgt cttcgactgg aagaaatttt gaagaaaatt cagcgacgtt
     1021 ttgactatca gcaagtgatt actccacata ttggaaacaa acaattatat attacttctg
     1081 gacattatgc caaatatgga aaagattcat ttcaacctat ccatactcct gaagaaggcg
     1141 aagaatattt gctaaaaccc atgaactgtc ctcatcattg cgaaatttat aaattcaaac
     1201 ctcgttctta taaagattta cctttacgaa ttgccgaatt tggaacagtt taccgttatg
     1261 aacaaagtgg tgaacttcat ggacttacac gcgtaagaag ttttactcag gatgatgctc
     1321 acatattttg tcgtcccgag caagtgaaag atgaattttt gaaagtaatg gatattattt
     1381 tcattatttt taaagctttg gattttaata actttgaagc tcaaatttct ttacgcgatc
     1441 caaataataa agaaaaatat attggtactg atgaaaattg ggataaagca gaaaaagcga
     1501 ttgtagaagc atgtgaagaa aaaggattga aagcaagggt tatgttggga gaagctgctt
     1561 tttatggacc gaaacttgat tttatggtaa aagatgctat tggtagacga tggcaattgg
     1621 gaactattca agtggattat aatcttccag aacgatttga gttggaatat acaggtgaag
     1681 ataaccaaaa acatcgccct gttatgattc atcgagcacc ttttggctcg atggaacgct
     1741 ttgttgcttt attgattgaa cataccgcag ggaaatttcc attatggctt gcgcccgatc
     1801 aagttgtgat attacccatc agtgaaaaat ataatcaata tgccttcgaa gtagaaaaaa
     1861 ttctaaagga aaaatctata cgagctatag tagatgatcg taacgaaaaa attggccgaa
     1921 aaattcgaga taatgaacta aaacgaatcc cttatctgtt aatcgttgga gagaaggaac
     1981 ttactggaag agaagtttcg gttcgtcgtc aaggtgaagg agataaagga actatgaaaa
     2041 ttgaggattt tgctcaatta attcttaatg aaatcaatat cttattaaaa cagtttgatt
     2101 aatttcattc tgttttaaaa aaataacaaa atatttgaat cagctttagt tttttaaact
     2161 tttgctgaat ttattagaca ttatatcatt tagattgcta tggtgttact gatcatagaa
     2221 attctgaaat tatatagacc acatgcaagt agcatgactt tatcttcaaa gaattgtttg
     2281 taatatctga atttgggctt aataattcgg gctcgttttg tatctccaat ggcatgttca
     2341 atagtaaccc ctctgagaag atatttcttt gttcagtttt tttctgctct ttggtcaaag
     2401 gtttaccttt tggttttttt aaaggcatta taccggattt tcctttcaaa aatggtatat
     2461 tttatatata acaaattgat tattacttgt ttaatatatt ttttaatgat tatccgtata
     2521 atgacctata tctggatatt ctgcttcacg aactaaattc cgatatctta atagattttt
     2581 aaagaacatg actatactac tctttgaaga taggatttgc tatatgataa attatatagt
     2641 tgaaagaaaa caacttccga atcgcaacta attaaaacat ttaagt
//
LOCUS       DAOTKS010000225         2667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_31184_length_2667_cov_3.334609, whole genome shotgun sequence.
ACCESSION   DAOTKS010000225 DAOTKS010000000
VERSION     DAOTKS010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31184_length_2667_cov_3.334609"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /gene="murG"
                     /locus_tag="P5236_05040"
     CDS             complement(<1..995)
                     /gene="murG"
                     /locus_tag="P5236_05040"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009237085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HRU63763.1"
                     /translation="MSKFIISGGGTGGHIFPAISIANALKRLQPEADILFVGAQGRME
                     MERVPAAGYPIKGLPISGINRKNIFKNIKVSIDLFRSMGLALKIVRQFHPEVVVGVGG
                     YASAPILMAANILGIPTVIQEQNSYAGITNKFLAKKASCICVAYEGMERFFPKDKIVL
                     TGNPVRQELLFIEPKKKEAYEFFGFQPNKKTLLILGGSLGARTINESVISHLEELAQS
                     DIQIIWQTGKYYLNEALKIAEKFLSPTFMITDFISRMDFAYAIADLVVSRAGASSISE
                     LSLLSKAAILVPSPNVAEDHQTKNALALVKKEAAIMIKDAEAKNTLIPRALEIIN"
     gene            complement(1142..2371)
                     /locus_tag="P5236_05045"
     CDS             complement(1142..2371)
                     /locus_tag="P5236_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRU63764.1"
                     /translation="MKELLKKYLRGDTTLWLVFLFLCIISVIEMYSASSTLAYKAAST
                     TVPILRHIGFLFGGALLVFFVHMIPYKYIRLLAYLGVILSIVLLIFVLLKGKSENDAT
                     RWIEIFGIQFQPSELAKLCVIIVTADLISRIKDKTTEKKYFLNILLMLAIICPLILFE
                     NFSTALLLFAVVFIMMFIGQISLKRLGILLGSLFVILVLAYFTIKIVPETSMPKVFDR
                     AYTWKARIDRYVSEKDKETEKYVITDENRQVQHGRIAIARGGVAGVFPGNSVERDYLP
                     QAYSDFIYAIIVEEMGLIGGIGVILLYLILLFRAGMIALKSPTVFPALLVMGLSLMIV
                     LQAFVSMAVATSLGPVTGQPLPLISRGGTSILITCIYFGIILGVTRQLKEEKNVSNSD
                     AENESEDLPVVKLEELN"
     gene            complement(2443..>2667)
                     /locus_tag="P5236_05050"
     CDS             complement(2443..>2667)
                     /locus_tag="P5236_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRU63765.1"
                     /translation="LVFLGVDNSPLHKFFDGKVAQIADAHSMEEAVRKAYEMATDGDT
                     VLLSPCCASFDLFQNYEDRGKQFKIYVRNL"
BASE COUNT          923 a          520 c          381 g          843 t
ORIGIN      
        1 tcatttatta tttctaaggc tcttggtatc aaagtatttt ttgcttcagc atctttgatc
       61 ataatagccg cttccttttt taccagtgcc aaagcatttt ttgtttgatg atcttcggca
      121 acattaggcg aaggtaccaa aatggctgct ttactcaaaa gacttaattc tgagatagag
      181 ctggctccag ctcgagaaac gactaaatca gctatggcat aagcaaaatc catccgggaa
      241 ataaaatctg taatcataaa agtaggtgat aaaaattttt ctgctatttt taatgcttca
      301 tttagataat acttgcctgt ttgccaaata atttgaatgt cactttgtgc taattcttcc
      361 agatgagaaa tgacactttc attaatggtt ctcgccccca aacttcctcc taaaattaac
      421 agcgtttttt tattgggctg aaaaccaaaa aattcatagg cttctttttt tttaggttca
      481 atgaaaagta attcctgacg aactggatta ccagttaaaa caatcttatc tttaggaaaa
      541 aatcgttcca tgccttcata agcaacgcaa atacaactgg cttttttggc taaaaattta
      601 ttggtaattc ccgcataaga attttgttcc tgaataacag taggaatacc taaaatattg
      661 gcagccatta gaataggagc actggcataa ccacccactc ctacaaccac ctcaggatga
      721 aattgacgaa caattttaag agccaaaccc atgctccgaa aaagatcgat tgaaactttt
      781 atatttttaa atatattttt cctatttata ccacttatcg gaagtccttt aataggataa
      841 cccgcagcag gaacacgttc catttccata cgcccttgtg ctcccacaaa caaaatatca
      901 gcctctggtt gcaacctttt taaggcattt gctatactaa ttgctgggaa aatatgtcct
      961 cctgtaccac caccactaat aataaatttg ctcattctat tatttactct attccactga
     1021 ttatataaac ttttcaagaa ataataaaac ttttcattct tcctaaaaaa ctcatttttt
     1081 tataatttat taaatttgaa taatttataa atggtatttt gatgcaaaat attatacaaa
     1141 tttaatttaa ttcttcaagt tttacaacag gaagatcttc actttcattt tcagcatcag
     1201 aatttgaaac gtttttttct tcctttagtt gccgagtgac gcccaaaata attccaaaat
     1261 agatacaagt aatcaatatg gaagttccac ctcgactaat caaaggaagc ggttgccctg
     1321 tcacaggtcc cagactggtg gctactgcca tacttacaaa tgcttgtaaa acaatcatca
     1381 gtgaaagtcc cattaccagc agtgcaggaa aaaccgttgg actctttaaa gcaatcattc
     1441 cagctcggaa cagaagtatt aaataaagta aaataacgcc gatgcctcca attaatccca
     1501 tttcttcaac aatgatggca taaataaaat cggaataagc ttgaggtaaa tagtcgcgtt
     1561 ctacactatt gccaggaaaa acacctgcta ctcctcctcg tgcaatagct attcgcccgt
     1621 gttgaacttg cctgttctcg tcggtaatca catatttctc tgtttcctta tctttttcag
     1681 aaacatatct atcaatgcgt gctttccaag tgtaagctct atcaaatact tttggcatgg
     1741 aagtttcagg aacgatttta atagtaaaat atgccagaac taaaatgaca aacaaactcc
     1801 ccaataaaat tcctaatctt ttcaatgaga tttgtccgat aaacatcata ataaacacca
     1861 ctgcaaaaag taaaagagca gttgaaaaat tttcgaataa aatcaaagga caaataatag
     1921 ctaacattaa aagaatattt agaaaatatt ttttttcagt agttttatct ttaattctcg
     1981 aaatcaaatc cgcagttaca ataatcacac aaagtttagc taattccgat ggctgaaatt
     2041 gtataccgaa aatttctatc catcgggtag catcattttc acttttgcct ttcagcaaaa
     2101 caaatattaa caaaacaata gacaaaataa cgccaagata tgctaaaagt cggatatatt
     2161 tataaggaat catatgaaca aaaaaaacaa gtaaagctcc cccaaacaaa aaaccaatat
     2221 gtcgcaaaat gggaactgtt gtgctggcag ctttgtaagc cagcgtactg gaagcactat
     2281 acatttcaat tactgaaata atgcataaaa agagaaacac taaccacaaa gttgtgtcgc
     2341 ctcgtaaata ttttttaagt aattctttca tgtcttagct tattaatgca attttggaaa
     2401 gttcttataa atgtttatgc tggatttttc cttaaaaatg aattaaagat tcctgacata
     2461 aattttaaac tgtttgcctc ggtcttcata attttgaaat agatcaaaac ttgcacaaca
     2521 aggtgaaagt aaaaccgtat cgccatctgt agccatttca taagcttttc ttacagcttc
     2581 ttccatagaa tgagcatcag caatctgggc tactttccca tcaaaaaatt tatgaagagg
     2641 actattgtca acgcctaaaa aaactaa
//
LOCUS       DAOTKS010000226         2658 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_31307_length_2658_cov_5.112178, whole genome shotgun sequence.
ACCESSION   DAOTKS010000226 DAOTKS010000000
VERSION     DAOTKS010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31307_length_2658_cov_5.112178"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            553..1821
                     /gene="purD"
                     /locus_tag="P5236_05055"
     CDS             553..1821
                     /gene="purD"
                     /locus_tag="P5236_05055"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRU63766.1"
                     /translation="MNILILGSGGREHAIAWKISQSPNVTCLYIAPGNAGTSSIGINL
                     PIEVNDFESIKEAVKNFQINYVIVGPEDPLVKGIVDYFQQDEDLKKVYIIGPDKRAAQ
                     LEGSKDFAKNFMFRHGIPTAKYLSVTATNLEKGIDFLKKMCPPYVLKADGLAAGKGVE
                     IISELEKAQEELRIMLEGKFGTASQKVVIEEFLSGIECSVFVVTDGENYKILPEAKDY
                     KRIGEGDTGLNTGGMGAISPVSFADEAFMQKVEKKIIKPTIQGLLEEGIVYKGFIFFG
                     LMKVDSEPYVIEYNVRLGDPETEAILPRISSDLVDLLEGITHCNLSDKKLAIDPRFVA
                     TVILASGGYPEKYEKGKVITGLEKVKDSIVFHSGTAFKDGKMVTNGGRVLAISSYGKT
                     KEEALELSYRNAEKIQFENKYYRKDIGFDL"
     gene            1900..2586
                     /gene="cmk"
                     /locus_tag="P5236_05060"
     CDS             1900..2586
                     /gene="cmk"
                     /locus_tag="P5236_05060"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRU63767.1"
                     /translation="MKKIIVAIDGPSSCGKSTIAKELARDVGYVYVDTGAMYRAVALY
                     CLQNGWLKDSSLDEEALKKNIKSLKIEFKTNEKGETETYLNGRNVEKEIRSLEVANAA
                     SQISMLDFVRKEMVCQQQEMGKEKGIVMDGRDIGTVVFPEAELKIFLTATPEIRAQRR
                     MLEMLERGEKVTFEEVLKNIRERDERDSHRLESPLKKAEDAIVIDNSNLTKEQQRELV
                     KTLFLEKVLS"
BASE COUNT          927 a          341 c          530 g          860 t
ORIGIN      
        1 aattccccga tgcttgcgtc ggggtagctt ttgtttttat ttttttagaa attccagtgg
       61 cgaagccata aaattggcgt aatacctcgc tgcttgtggc gaggatagtc aatttttaag
      121 gaatttcttt actaaaataa aaatttttcc tgattatttt ctcttaaaat atttaaaatt
      181 tattttatga ttaagtgaag atttttttat aactttgaaa atcaaaccag aaaaaaatta
      241 tttcactcaa tgatactcat aacaagagga cattatttaa aatatgttat ataacatatt
      301 tttatttaaa tttatttgga gaagtttggt agttatttaa aaaagctata catttgcatc
      361 gtaatttttt tcatagtatt agattttaag gttaacaaaa aagattggta gtcaagcgtg
      421 actacctttt cttttttgta gaactttttg ttgttttttt actttctttt ctactcatca
      481 actttttcaa ccttcctgtt attttgttca actctaaaaa ttaaattatt tttgtacgaa
      541 aaataatttg ttatgaatat tctcatcctt ggttcaggtg gacgtgaaca tgccattgct
      601 tggaaaattt cgcaaagtcc aaatgtgact tgtctgtata ttgctccagg aaatgctgga
      661 acttcctcta taggaatcaa tttgcctatc gaagtaaatg attttgaatc aataaaagaa
      721 gctgtaaaga atttccaaat taactatgtg attgtaggac ctgaagatcc ccttgttaaa
      781 ggaattgttg attattttca acaggatgaa gatttgaaaa aagtttatat aattggtcct
      841 gataaaaggg ctgctcaatt ggaaggaagc aaagattttg caaaaaattt tatgtttcgt
      901 catggtattc ccactgcaaa atatttaagt gttaccgcca caaatctgga aaaaggaata
      961 gatttcttaa aaaaaatgtg tccgccttat gttttaaaag ctgatggctt ggcagcagga
     1021 aaaggtgtag aaataatttc tgaacttgaa aaagctcagg aagaattgag aataatgctc
     1081 gaaggaaaat ttggaacagc aagtcaaaag gtagtaattg aagaattcct ttctggaata
     1141 gaatgttctg tttttgtagt cactgatgga gaaaattata aaattttacc agaagccaaa
     1201 gattacaagc gcattggaga aggcgatacg ggtttaaata caggtggtat gggagcaatt
     1261 tctccagttt cgtttgctga tgaagcgttt atgcagaaag tagaaaaaaa gattattaag
     1321 ccaactattc aagggttgct tgaggaagga attgtttata aaggttttat attttttgga
     1381 ttgatgaaag tggacagtga accttacgtc attgaataca atgttcggtt gggtgatcct
     1441 gaaacggaag ccattttgcc tcgtatttct tctgatctgg tagatttgtt ggaaggaatt
     1501 acccattgta atttgagtga caaaaagctg gcgatcgatc ctcgctttgt agcaacagtt
     1561 atattggctt caggtggtta tcctgaaaaa tatgagaaag gaaaggtaat cactggttta
     1621 gaaaaagtaa aagatagtat tgtctttcat tctggtactg cttttaaaga tggtaaaatg
     1681 gtaactaacg gtggacgagt gttggcaatt agctcttatg gcaaaacaaa agaagaagca
     1741 ttggaattat cttatcgaaa tgctgaaaag attcagtttg agaataaata ttatcgaaaa
     1801 gatataggtt ttgatcttta aaaataggaa taatacacat aatttcctga taatctcctc
     1861 ctttttctct ttttaacaaa ataatgaacg aaatcaatca tgaaaaaaat aattgttgct
     1921 attgatggac cttcatcgtg cggaaaaagt accattgcca aagaattggc tcgtgatgtg
     1981 ggatatgttt atgtcgatac aggagcgatg tatcgagcag tagctttata ttgtttacaa
     2041 aatggctggt taaaggattc ttcacttgac gaagaagctt taaagaaaaa tataaaatct
     2101 ctcaaaattg aatttaaaac caatgaaaag ggagaaacag aaacttattt gaatggcagg
     2161 aatgttgaaa aagaaattcg ttcgttagaa gtagcaaatg cggcaagtca aatcagtatg
     2221 ttagattttg ttcggaagga aatggtttgt cagcagcaag aaatggggaa agagaaagga
     2281 attgtgatgg atggtcgtga tataggaacg gttgtttttc ccgaagcaga attaaagatt
     2341 tttttgactg ccacacctga aatccgtgct caaaggcgca tgctagaaat gctggaaaga
     2401 ggtgagaagg taacttttga agaagttttg aaaaatatcc gtgaacgtga tgaaagagat
     2461 tcacatcgat tggaaagtcc tttaaaaaaa gcagaagatg ctattgtaat tgacaattct
     2521 aatcttacaa aggagcaaca acgagaattg gtaaaaactt tatttttaga aaaggtgtta
     2581 tcataaaaaa taagataatt ttacactact gaccgacaaa agataagata aaaaagaagg
     2641 gacaagatga aagtctcg
//
LOCUS       DAOTKS010000227         2636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_31665_length_2636_cov_3.590081, whole genome shotgun sequence.
ACCESSION   DAOTKS010000227 DAOTKS010000000
VERSION     DAOTKS010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31665_length_2636_cov_3.590081"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="P5236_05065"
     CDS             <1..855
                     /locus_tag="P5236_05065"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRU63768.1"
                     /translation="VRGLSVGNNVRFAGINVGTVKNIRIVSDTSVIVTFSVNNRYTKY
                     IYKNSTVQIGQEGLMGGKIVLILPGDQQTGQVQEGDFLKVAEGLDIQGMLAQTQAMLQ
                     EATGTVSHLRSIMAKVDSGQGDIGNLINHDSLTMQLNLAMNNVNSAIAHVKQLISKID
                     SGQGDLGKLVNDDKLTRKLNKILSDVDSVIYELHTTTYALNHGEGTLPRLVHDKQMAQ
                     NIDTAVAKVDEAVVQVTRAAETVANSWIFRLFSKKKKEPKEQPVQEFKVQPGDTIVID
                     SVGAKLKQ"
     gene            complement(869..1588)
                     /gene="rsmI"
                     /locus_tag="P5236_05070"
     CDS             complement(869..1588)
                     /gene="rsmI"
                     /locus_tag="P5236_05070"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRU63769.1"
                     /translation="MNHSAQEDHHSIKNNKTKLYLVPTPVGNLEDMTLRAIRILKEVD
                     MILAEDTRTSSILLKHFDIKTPLLSHHKFNEHQTVDNVVQQLLSGQTIALISDAGTPA
                     ISDPGFLLVRKCIENDIEIECLPGATAFVPALVVSGLPADRFCFEGFLPIKKGRQNRL
                     KELASENRTMIFYESPFRLLKTLTQLAETLGNERKASISREISKFFEETKRGTFHELI
                     DYFTENSPKGEFVIVVAGKEE"
     gene            complement(1615..2448)
                     /locus_tag="P5236_05075"
     CDS             complement(1615..2448)
                     /locus_tag="P5236_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63770.1"
                     /translation="MNREIILLLLRKNIHDLDLLTESLSEMVEFPPVLLHMIQQKVKE
                     IQDYVDQLIAEEITIVETEKESEIFEAQTNEKSTQTSEKEEKIENEVANFQIEKENSV
                     NETIISSEIVENISFEPQKEEMIEYVSDETTPEIGKENDIFPEESEEKISKEKILETE
                     MEEQTTSISPSETINKEKTIISKSTLSQLFPQPENNIEATLSHQKIDDIKKAISIGDR
                     FRFQRVLFKGNGEDMNKTLNYLNQLATFEEAVSFLEAKYHWEKDNETAEDFYRILKRR
                     F"
BASE COUNT          825 a          414 c          469 g          928 t
ORIGIN      
        1 gttcggggtt tgtcagtagg caataatgtt cggtttgccg gtattaatgt aggaaccgtg
       61 aaaaacatac gtattgtttc ggatacctct gtcattgtta ctttttcggt aaataatcgt
      121 tatacaaaat acatttacaa aaattctact gttcaaattg gacaagaagg tttaatgggt
      181 ggaaaaattg ttctcatttt acccggtgac caacaaacag gacaggtaca agaaggggat
      241 tttttaaaag tggcagaagg acttgatatt caagggatgc tggctcaaac acaagcaatg
      301 ctgcaagaag ctacaggcac agtgtcccat ttgcgttcca taatggctaa agttgacagt
      361 ggacaaggtg atatcggaaa tcttatcaac catgattcac ttactatgca attaaatctt
      421 gctatgaata atgttaattc agcgatagct catgtaaaac aactgatcag caaaattgat
      481 agcggacaag gcgatttggg gaaattagtt aatgatgata aactaactag aaaattgaat
      541 aaaattttaa gcgatgttga ttctgttata tatgaattgc atacgactac ttatgcattg
      601 aatcatggtg aaggaactct tcctcgcctc gtgcacgata agcaaatggc acaaaatatt
      661 gatactgctg tagcaaaagt tgatgaagct gttgttcaag tgaccagagc tgctgaaact
      721 gtagccaata gctggatttt ccgtttgttc tccaaaaaga aaaaagaacc caaagaacaa
      781 ccagtacaag aatttaaagt acaaccagga gatactattg taatcgatag tgttggagca
      841 aagttaaaac aatgatgtaa cgaataggtt attcttcttt tccagcaacg acaataacaa
      901 attctccttt tggcgaattt tcagtaaaat aatcaattaa ttcgtgaaaa gtacctcgtt
      961 tagtttcttc aaaaaatttg gatatttcac gcgagatgga agcttttcgt tcatttccca
     1021 gcgtttcggc aagttgagtt aaggttttta atagtcgaaa aggtgattca taaaaaatca
     1081 tggtacgatt ttcagaagcc aattctttta atctattttg acgtcctttt ttaatgggca
     1141 aaaaaccttc aaagcaaaat cggtctgcgg gcaagccaga aacaacaagt gctggtacaa
     1201 aagcagtggc accgggcaag cattctattt caatatcatt ttctatgcac ttcctcacca
     1261 gtagaaaacc tggatcggaa atagcaggtg tgcctgcgtc ggaaattaat gcaatggttt
     1321 gtccactcaa aagttgctgc acaacattat cgactgtttg atgttcatta aatttgtgat
     1381 gggagagtaa gggcgttttt atatcaaaat gtttgagtaa aatactactg gtacgggtat
     1441 cttctgccag aatcatatcc acttctttca gtatgcggat agctcgtaaa gtcatatctt
     1501 caaggttccc gacaggtgta ggaacaagat ataattttgt cttattgttc ttgattgaat
     1561 gatgatcttc ttgggctgag tgattcatag caattatttt tttattacaa cgctttaaaa
     1621 tcttcttttc aagattctgt aaaaatcttc ggcagtttcg ttatcttttt cccaatgata
     1681 ttttgcttcc agaaatgaga cagcttcttc aaaggtagcc agttgattta aataattgag
     1741 tgttttattc atatcttctc cattgccttt gaaaagtact cgttgaaatc ggaaacgatc
     1801 accaatgctg atggcttttt ttatgtcgtc aattttttga tgcgacaaag tagcttctat
     1861 attattttca ggttgaggga acaattgaga aagggttgat ttggaaataa tagttttttc
     1921 cttattaata gtttctgagg gtgaaataga agttgtttgt tcttccattt ctgtttctaa
     1981 aatcttttct ttcgaaattt tctcttcaga ttcttctgga aaaatatcat tttctttccc
     2041 tatttcagga gtagtttcgt ctgaaacgta ttctatcatt tcttcttttt gcggttcaaa
     2101 agatatattt tcaacgattt cgcttgaaat gatggtttca ttcacggaat tctctttttc
     2161 tatttgaaaa tttgctactt cattttctat tttttcttct ttttctgacg tctgggttga
     2221 tttttcattt gtttgagctt caaaaatttc tgattctttt tctgtttcta ctattgttat
     2281 ttcttcagcg attaattgat ctacataatc ttgaatttct ttcacttttt gttgaatcat
     2341 atgaagtaaa acaggtggaa attcaaccat ttcgctcaaa ctttcggtga gcaaatctaa
     2401 atcatgaata ttttttctca aaagaagaag aataatttct ctgttcatat ttttgctaaa
     2461 aacaatttta atatttatta ttttttttaa ttttgtcaga aattacaaat aaatatcttt
     2521 gtaattttca aaattttttg aaaaagataa aaaactttgt taggaacaac aaactttaag
     2581 tggtgtaaag ttacaacaat tgagagagaa ataaaaaaga aattttaatt ttcaac
//
LOCUS       DAOTKS010000228         2613 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_32020_length_2613_cov_3.896403, whole genome shotgun sequence.
ACCESSION   DAOTKS010000228 DAOTKS010000000
VERSION     DAOTKS010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32020_length_2613_cov_3.896403"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..185)
                     /locus_tag="P5236_05080"
     tRNA            complement(103..185)
                     /locus_tag="P5236_05080"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(149..151),aa:Tyr,seq:gta)
     gene            complement(234..307)
                     /locus_tag="P5236_05085"
     tRNA            complement(234..307)
                     /locus_tag="P5236_05085"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(271..273),aa:Thr,seq:tgt)
     gene            complement(418..720)
                     /gene="raiA"
                     /locus_tag="P5236_05090"
     CDS             complement(418..720)
                     /gene="raiA"
                     /locus_tag="P5236_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HRU63771.1"
                     /translation="MKLRIQAINFEATSALESYVNKKLAKLEKLTDDIFNVEVYLKLL
                     KSSTGANKEVEIKVSIPSVEFFSSKVCETFEEATDLCIDAIEKQIKKHKEKFNYKK"
     gene            complement(734..1615)
                     /locus_tag="P5236_05095"
     CDS             complement(734..1615)
                     /locus_tag="P5236_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRU63772.1"
                     /translation="MIKDFLDYLKYEKNYSSHTILSYKTDLLQFCDFLNTTPEALNVS
                     EVTSMQIQQWIASMMSEKKSKRTISRKISTLKSFWHFLLERKVVNQNPTLRIILPKIK
                     KPLPSFFKNEEMEKVLDETFLLENFEAQRDHLILEIFYLTGIRLSELISIQDTDIDLK
                     EGILRVIGKRNKERMIPLSKKLCEDIEHYIQLRDQQIEKKGNWLFVRKNGEKLYPKWV
                     YNKIHRTMSDVSTLNKKSPHVLRHSFATALLNEGADINAVKELLGHSSLAATQVYTHT
                     SFKELYNIYKQTHPRAK"
     gene            complement(1685..1876)
                     /gene="rpsU"
                     /locus_tag="P5236_05100"
     CDS             complement(1685..1876)
                     /gene="rpsU"
                     /locus_tag="P5236_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006950716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRU63773.1"
                     /translation="MIIIPVKEGENIDKALKKYKRKFEKTGVVRELRRRQSFEKPSIT
                     KRNQKLHAVYVQKLQSENQ"
     gene            complement(2068..>2613)
                     /locus_tag="P5236_05105"
     CDS             complement(2068..>2613)
                     /locus_tag="P5236_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRU63774.1"
                     /translation="QAAMEGFEVCTVEDALPEGNIYVTATGNRDIITIQHLEKMKDGA
                     IVCNIGHFDNEIQVDQLKKFPGIQKVNIKPQVDKYIFPDGHSILLLADGRLVNLGCAT
                     GHPSFVMSNSFTNQTLAQIELFTKKYKVNVYRLPKHLDEEVARLHLDQLGVKLTHLTK
                     EQAEYIGVNPGGPFKPEHYRY"
BASE COUNT          803 a          454 c          403 g          953 t
ORIGIN      
        1 cgcgaccctc agcttggaag gctaatgctc tatcaactga gctacttccg caaaagattt
       61 attgaattcc cctcaaaatg agttcaataa atttttcctc agtgggcagt gatggattcg
      121 aaccaccgaa gtcgtcagac agctgagtta cagtcagccc caattgtcca ctctggcaac
      181 tgcccaaaat gataaagaac ttttaaattg taagctcact ataaataact ttgagcctct
      241 tgtaggattt gaacctacga ccccgagatt acaaatcacg tgctctggcc aactgagcta
      301 aagaggcgaa ttacttataa tatcctcttt tactgtttaa aaagccgttt aaataaacct
      361 acttaaacgg cttgcaaatt tacataaata attttgaatt ccaaaaacta caatcaatta
      421 ttttttgtaa ttgaactttt ccttgtgctt tttaatttgt ttttcgatag catcaataca
      481 caagtcagta gcttcttcaa aagtttcaca cactttagat gaaaaaaact ctacacttgg
      541 aatggaaact tttatttcta cttctttatt ggcacccgta cttgatttta aaagctttaa
      601 atatacttcc acattgaaaa tatcatctgt tagcttttct aactttgcca attttttatt
      661 tacatacgat tctaatgctg aggttgcctc aaaattaata gcttggattc ttaatttcat
      721 aatttaactt cttttatttc gctcttggat gggtttgttt gtatatgtta tataattctt
      781 taaaacttgt gtgagtataa acttgcgtag ctgcaagact gctgtgtcct aacaattctt
      841 ttacagcatt aatatcggca ccttcattga gtaaagcggt ggcaaaacta tgccgcaata
      901 catgtggact ttttttattt aaggtactga catccgacat cgttcgatga attttattat
      961 atacccattt tggatataat ttttcaccat tttttcgtac aaaaagccaa ttaccttttt
     1021 tttctatttg ctggtcacgc agttgaatat aatgttcaat atcttcacat aatttttttg
     1081 aaaggggaat cattcgttct ttgttgcgtt ttccaatcac acgcaaaatg ccttctttca
     1141 aatcaatatc ggtatcttga atagaaataa gttctgataa acgtatacct gttaaataaa
     1201 aaatttctaa gattaaatga tcccgttgtg cttcaaaatt ttcaagtaaa aatgtttcgt
     1261 ccaaaacttt ttccatttct tcgtttttaa aaaaagaggg caaaggtttt tttattttgg
     1321 gcaaaataat tctcaacgtt ggattttgat tgactacttt tctttccaat aaaaaatgcc
     1381 aaaaagattt taaagtggaa attttacgtg aaatggtacg ttttgatttt ttttctgaca
     1441 tcattgaggc aatccattgt tgaatttgca tcgaagttac ctcagaaaca tttaacgctt
     1501 caggagtagt gtttaaaaag tcgcaaaatt gtaacaaatc ggtcttatat gataaaattg
     1561 tatgagaaga ataatttttt tcatacttta aatagtctaa aaaatctttt atcatgacct
     1621 tataagttca attttgcaac gactgtaaat taaaaaacca aaaaacaact ttttaaggta
     1681 gtttttattg gttttcactt tgcaattttt gtacgtaaac agcgtgtaat ttttgattac
     1741 gttttgttat agaaggcttt tcaaagcttt gacgacgacg aagttctctg acaacacctg
     1801 ttttttcaaa tttgcgtttg tactttttta atgctttgtc gatgttttca ccttctttta
     1861 caggaatgat aatcataatg ctttatttct tgattttttg atgttaaatt tttgggttca
     1921 actttaaaat gaggttgcaa agataaaatt ttttttcaaa attaccaact gatttgataa
     1981 aatctatatt gaaataatta ttcagtttat aagataaaaa tctaaaaata ttaccactaa
     2041 gaacccttag tttattatta taacaattta ataccgataa tgttccggct taaaaggtcc
     2101 tcctggattc acgccaatat attcagcctg ttctttggta agatgggtaa gctttactcc
     2161 taattggtct aaatgaagtc gggctacttc ttcgtccaga tgtttgggca gacgatacac
     2221 atttaccttg tatttttttg taaacagttc aatttgagcc agcgtctggt tggtaaaaga
     2281 attgctcatc acgaaagaag gatgacctgt tgcacagcca agatttacca gacgtccgtc
     2341 agcaagaagc aaaatactgt gtccatcagg gaaaatatac ttgtccacct gaggtttgat
     2401 attgactttt tgtataccag gaaatttttt cagctgatct acctgaattt cattatcgaa
     2461 atgtccgata ttgcagacaa tggctccatc tttcattttt tccagatgtt gaatggtgat
     2521 aatatcgcga ttgccggtgg cagtaacata aatattccct tctggtaaag cgtcttctac
     2581 tgtgcaaact tcaaatcctt ccatagcagc ctg
//
LOCUS       DAOTKS010000229         2604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_32154_length_2604_cov_4.522166, whole genome shotgun sequence.
ACCESSION   DAOTKS010000229 DAOTKS010000000
VERSION     DAOTKS010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32154_length_2604_cov_4.522166"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..1400
                     /locus_tag="P5236_05110"
     CDS             30..1400
                     /locus_tag="P5236_05110"
                     /inference="COORDINATES: protein motif:HMM:NF020855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRU63775.1"
                     /translation="MSRKIFFQSDDDINEVVYRYEQFLSGKASGYFDIEEFENIAEYY
                     LQKGRTKDSSRAIEFGIKLHPTSSELKTKRAKIYLASGNAEKAYQLLESLADKNDYEV
                     ILLKIQALVHMRRLSEAYQLCNRVIDFENEGKDDVLLDIAYIYIEHGYFNEAMEWLLK
                     GYKINPKNSDLLFELAFCYEQLDEDEKAIVIYNKIIDLDPYSSEAWFNLGQIYFTSQE
                     FSKAIEAYDFVLAIDENDSLACLQKAHAHFQLNQFKEAIENYKEYAELTSDSEQANIY
                     IAESFEKMDQFDDAISYYQRSLKIQPDNYDALLGIGACLLEKEKFKESIQFISKALHI
                     NKEDTDAWNYLGEDYLRMDDIDNALLCYLKSITLDPSQPETLMIIANINLEKGDYNTA
                     LEFYLQVLQQDSTIEYLYLFLAITYYKIGNKTLAYAYLKKAIQENEEASSMFLEICPE
                     AIDPIL"
     gene            1603..2160
                     /locus_tag="P5236_05115"
     CDS             1603..2160
                     /locus_tag="P5236_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009229923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HRU63776.1"
                     /translation="MTIESSFIPFPSEIIVPPAAYIASQPDSNLNHLLIILFATLGSL
                     MGAYINYALSISLGRALIYKLADSKVGAALLLNSEKIRKAEAYFNKYGNISTFIGRLI
                     PGIRQLISIPAGLAKMNFFKFSLYTVLGAGVWNCVLVYIGYLAHGQADLINKYNKEIS
                     IVMVILVVAAGIFGGIKYYLKLKKK"
     gene            2157..>2604
                     /gene="aroE"
                     /locus_tag="P5236_05120"
     CDS             2157..>2604
                     /gene="aroE"
                     /locus_tag="P5236_05120"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272587.1"
                     /note="AroE; catalyzes the conversion of shikimate to
                     3-dehydroshikimate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRU63777.1"
                     /translation="MKLYGLIGYPLTHSFSKKYFTEKFEKENIDARYNLYELENIDLF
                     PELKNIPELAGLNVTIPYKEQIIPYLDELDDTAKEIGAVNVIKFIRNGKILKLKGYNT
                     DVIGFEKSILPFLQPYHRKALILGTGGVSKAIYFVLQKLGIETTFVS"
BASE COUNT          901 a          392 c          435 g          876 t
ORIGIN      
        1 aaaatattaa ttttgcaaaa aaataaagca tgagcagaaa aatatttttt caaagcgatg
       61 atgatatcaa tgaggtagtt tatcgttatg aacaattttt atctggaaaa gcatcaggtt
      121 attttgacat tgaagaattc gaaaatatcg cagaatatta tttacaaaaa ggtcgtacta
      181 aagattcttc cagagctata gaatttggaa ttaagcttca tcccaccagc agcgaattaa
      241 aaactaaacg ggctaaaata tatcttgctt ctggaaatgc agagaaagct tatcaacttt
      301 tggagtcgct tgctgataaa aatgattacg aagttatttt gcttaaaatc caagcattgg
      361 tgcatatgag acgtttatcc gaagcttatc aattatgtaa ccgcgtaatt gattttgaaa
      421 atgaaggaaa agacgatgtc cttctcgaca ttgcctacat ttatattgag cacggatatt
      481 tcaacgaagc aatggaatgg ctgctaaaag gttataaaat caatcctaaa aatagcgact
      541 tgttatttga actggctttc tgctatgaac aattggacga agacgaaaaa gcaatagtga
      601 tctacaacaa aatcattgac ctcgatcctt attcttcaga agcatggttc aatttgggac
      661 aaatttattt tacttctcaa gaattttcaa aagctattga agcgtatgat tttgttttgg
      721 caattgacga aaatgattct cttgcttgtt tgcaaaaagc tcatgctcat tttcaactaa
      781 atcagttcaa agaagccatt gaaaattata aagaatatgc tgaattgaca agcgacagtg
      841 aacaagccaa tatttatatt gcagaaagtt tcgagaaaat ggatcaattt gacgatgcaa
      901 tttcttatta ccaacgttcc ctgaaaattc aacctgacaa ttatgatgca ctgttaggta
      961 ttggagcttg cttattggaa aaagaaaaat tcaaagaaag catacaattc atttccaaag
     1021 cgttacatat taataaagaa gatacggacg cctggaatta tctgggagaa gattatttga
     1081 gaatggacga cattgacaat gctttgcttt gttatttgaa atctattaca ttagatccaa
     1141 gtcaaccaga aacattgatg ataattgcca atatcaatct ggaaaagggt gattacaata
     1201 cagcactgga attttatctt caagtattac aacaggattc taccatagaa tatctttatc
     1261 tgtttttagc cattacttat tacaaaatcg ggaataaaac gctggcttat gcttacttga
     1321 aaaaagccat tcaagaaaat gaagaagctt ccagcatgtt tttggaaatt tgtcccgaag
     1381 caatagatcc tattctttaa tatgaacatt tagtatcatt ttatgaaaaa taaacctatt
     1441 attcttcttg ttttcttgtt tactttttta tcatttaata tacaaacaac tgaagctcaa
     1501 aaaaatatta gtaatttaaa aacggttcaa gaactttctt tacttcaaaa aatagaaaga
     1561 tggtataaca aaaatatgac ttatagtgct atttgtgctc ttatgactat tgaaagttct
     1621 tttatccctt ttccttctga aattattgtc cccccggctg cttatatcgc tagccaacca
     1681 gatagtaatt taaaccacct cttaattatt ttgtttgcaa cattgggttc actgatgggt
     1741 gcttatatca attatgctct ttccatttcg ttgggaaggg ctttgattta caaattggct
     1801 gacagtaaag ttggagctgc tctacttttg aatagtgaaa aaattcgtaa agccgaagct
     1861 tactttaata agtatggaaa tatttccact tttattggaa gattaattcc aggtattcgt
     1921 caactaattt ctatccctgc tggcttagca aaaatgaatt tttttaaatt ttctttatat
     1981 actgttttgg gagctggagt atggaattgt gtgctggttt atattggata tctggctcat
     2041 ggacaagccg atttgattaa taaatacaac aaagaaatca gcattgttat ggtaatatta
     2101 gtagttgcag cagggatttt tggtggaatt aaatattatt tgaagctaaa gaaaaaatga
     2161 aactgtacgg attaattggt tatccattga ctcattcttt ttcaaaaaaa tattttactg
     2221 aaaaattcga aaaagaaaat attgatgctc gatataattt gtatgagtta gaaaatatcg
     2281 atttatttcc tgaattaaaa aatattcctg aacttgctgg tttgaatgtt actattcctt
     2341 ataaagaaca aattattcct tatcttgatg agctggatga tactgcaaaa gaaattggag
     2401 ctgtaaatgt aataaaattc attcgcaatg gtaaaatctt aaaattaaaa ggttacaaca
     2461 ccgatgtgat aggttttgaa aaatccatcc ttcctttttt acagccctac catcgtaaag
     2521 ccttgatctt aggtacagga ggcgtttcaa aagccattta ttttgtcctt caaaagcttg
     2581 ggattgaaac tacttttgtt tcac
//
LOCUS       DAOTKS010000230         2583 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_32492_length_2583_cov_3.662184, whole genome shotgun sequence.
ACCESSION   DAOTKS010000230 DAOTKS010000000
VERSION     DAOTKS010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32492_length_2583_cov_3.662184"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /gene="dapF"
                     /locus_tag="P5236_05125"
     CDS             complement(<1..541)
                     /gene="dapF"
                     /locus_tag="P5236_05125"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRU63778.1"
                     /translation="MKFTKMHGIGNDYIYINGFEEKIKNPNELSIRLSDRHKGIGADG
                     LVLILPSSVADFQMQMFNADGSEGEMCGNAARCIGKYVYEKGLTDKTELTLETLAGIK
                     KLKLFLNDKQKVEKVTVDMGEPILEAEQIPVLFQKSPIVNEPVDFGNGINYAITCVSM
                     GNPHAVIFTDNIDQLDIKTI"
     gene            complement(719..1738)
                     /locus_tag="P5236_05130"
     CDS             complement(719..1738)
                     /locus_tag="P5236_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HRU63779.1"
                     /translation="MKCFASDNYSGIHPEILNAIIKANVDHETSYGEDSYTMQAQKVF
                     ESIFGDVDVLYAFNGTGANIVSLKCCTLPFQAVICAETAHINTDECGAVAQNIGCSLL
                     TVPTPDGKLTPEMIEPFLYRIGNVHNSQPKVISISQSTELGTIYSVSELKALCKFAHE
                     NNLYVHIDGARISNAVAALNVGLKEATVDCGVDIMSFGGTKNGLMLGEAILIFNSELR
                     KNAAFYQKQSMQLYSKNRFIAAQFIAFLSNNLWLQLALHSNQMAQMMAEKLQTIPEIK
                     ITQPVQTNSIFVIIPEKIIEPLREKYKFYTWNEQTNELRWMCSFDTIEKEIDDFVETI
                     RQLVE"
     gene            complement(1787..>2583)
                     /locus_tag="P5236_05135"
     CDS             complement(1787..>2583)
                     /locus_tag="P5236_05135"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRU63780.1"
                     /translation="LQNKIIEMYLTGLLILGIVAGILSGLFGIGGGIVLVPCLLVIFG
                     LDILHANATSLTAMLLPVGILGVWEYYKAGQINIKKSLWIALGLFLGSFVGGEIAVNI
                     SETFLTRLYAFILLCIAISYFDLPKYFHKKNEIIVKEDPVISKSFWMYILVGVAAGIF
                     AGLFGKGGGIIIVPLLIHFFQYDAKKAVATSLASLQLPVGLPSVIIYAKEGYLNLLFA
                     ALIAGGMVVGAFLGSKIGIRISSEIFKKVYGVFLLVVAIYLAIKYI"
BASE COUNT          902 a          486 c          358 g          837 t
ORIGIN      
        1 ctatcgtttt aatatcaagt tgatcaatat tatctgtaaa aatcacggca tgcgggtttc
       61 ccattgaaac acaagtaatg gcataattta ttccattccc aaaatctact ggttcattca
      121 caataggtga tttttgaaaa agaacaggaa tttgttcagc ctcaagaatt ggttctccca
      181 tatccactgt cactttttca actttctgtt tgtcatttaa aaataatttg agttttttga
      241 tacctgccag cgtttccagc gttaattcag ttttgtctgt taatcctttt tcataaacat
      301 atttaccaat acaccgggca gcgtttccgc acatttcacc ttctgaacca tcagcattaa
      361 acatttgcat ctggaaatct gccacagagg aaggcaaaat taataccaga ccatcagctc
      421 caatgccttt gtgacgatcg ctcaagcgaa tagaaagttc attgggattt ttaatttttt
      481 cttcaaagcc attaatataa atataatcgt taccgatgcc gtgcatttta gtaaacttca
      541 taatctgtag cttttaaaca tataaacaat ttttcctttt tataaaaatt ccttcacaag
      601 aaggagaaaa gatcaagatt gcaaaaatac tcattaattc aaataatttt gtaaaatttg
      661 gaatgatttc agaaaacaca atattaaatt gttgatttta aagtttttaa gatttggttt
      721 attcaactaa ttgccttatg gtttcaacaa aatcatcaat ttccttttct atcgtatcaa
      781 aggaacacat ccagcgtaat tcgtttgttt gttcattcca ggtgtaaaat ttgtatttct
      841 cacgcaatgg ttcaattatc ttttcaggaa taattacaaa tatcgaatta gtttgtaccg
      901 gttgtgtaat ttttatttca ggaatagtct gtaatttttc tgccatcatt tgtgccattt
      961 ggttggaatg caatgccaac tgcaaccata agttattgga taagaacgca ataaattgag
     1021 ctgcgataaa acggttttta gaataaagtt gcatggattg cttctgataa aaagctgcat
     1081 tttttcttaa ttcactatta aaaatcaaaa tggcttcccc caacattaaa ccattttttg
     1141 taccgccaaa actcataata tctacgccac aatctactgt agcttctttc aaacctacat
     1201 tcaacgcagc aacagcattt gaaatccttg ctccatcaat atggacataa agattgtttt
     1261 catgggcaaa tttacataat gcttttaatt ctgaaactga atagattgtt cccaattctg
     1321 tactttgact gatagaaatc acttttggct gagaattatg aacatttcca attcgataca
     1381 aaaaaggttc aatcatttca ggagttaatt tgccatcggg agttggaacg gttaaaagag
     1441 aacaaccgat attttgagct actgctccac attcatcggt attgatgtga gctgtttcag
     1501 cacaaatgac tgcttgaaaa ggtaaggtac aacatttcaa actgacaata ttagctcctg
     1561 tgccattaaa agcatataac acatcaacat cgccaaaaat actttcaaaa actttttgag
     1621 cctgcatcgt ataactatct tcaccatacg aagtttcatg atcaacattg gctttgataa
     1681 tggcatttaa gatttcagga tgaattccag aataattatc gctggcaaaa catttcataa
     1741 ttaaataatt tattaaaagt tttgtaatta attcatttac acataattat atgtatttaa
     1801 ttgccaaata aatagcaact actaataaaa aaacaccata aactttttta aatatttcag
     1861 atgagattct aatccctatt tttgatccta aaaaagcacc aaccaccatt ccaccagcaa
     1921 taagtgctgc aaatagtaaa ttcaaatatc cttctttggc ataaataatt acactgggta
     1981 aacctactgg taactgtaaa gatgccaatg aagtggcaac tgcttttttt gcatcatatt
     2041 gaaaaaaatg aatcagcaat ggtacaatga taatacctcc tcctttccca aaaagtccgg
     2101 caaaaattcc agcagctacg ccaactaaaa tatacatcca gaaagattta gaaatgacag
     2161 gatcttcttt tacaataatt tcgtttttct tatgaaaata ttttggtaaa tcaaaatagc
     2221 taatggctat acataataag ataaatgcat aaagtcgcgt aagaaaagtt tcactgatat
     2281 tgactgcaat ttcgcctcca acaaaagagc ctaaaaacaa tcccaatgca atccataatg
     2341 attttttgat attgatttga cctgctttgt aatattccca cacccctaaa atgccaacag
     2401 gcaacaacat agcagtaagt gaagtggcat tggcatgaag aatgtctaat ccaaaaataa
     2461 ccagcaaaca cggcactaat acaataccac ctccaatacc aaataaaccg gataaaattc
     2521 cagcaacaat tcccagaatc aataaacccg ttaaatacat ctcgatgatt ttattttgca
     2581 aaa
//
LOCUS       DAOTKS010000231         2580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_32537_length_2580_cov_4.133465, whole genome shotgun sequence.
ACCESSION   DAOTKS010000231 DAOTKS010000000
VERSION     DAOTKS010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32537_length_2580_cov_4.133465"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="P5236_05140"
     CDS             <1..1023
                     /locus_tag="P5236_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HRU63781.1"
                     /translation="PTTVGYTKNDIQNWCESLARCLTMAGVTKEDVVQVSYGYGLFTG
                     GLGLHYGAETIGATVIPASVGNTKRQLQLMSDYGVTVLACTPSYALHLVDAMSEFGYS
                     RADMKLRLGIFGAEPWTENMRLELERKWGIQAFDIYGLSEIMGPGIAHDCSFHQGLHI
                     NEDHFFPEIVDPKTKQPLPDGCEGELVFTTLTKEGIPLLRYNTRDLSMLNHEKCECER
                     TLVRMKKITGRSDDMLIIRGVNLFPSQIEQVLLEMCETSAHYLLCVDRKDNLDYLELK
                     VELDESKMVETIRELESLSKRIEHSLRSAIGLSIKVTLVEPRSIPRSEGKAVRVIDNR
                     KKEESK"
     gene            1108..2418
                     /locus_tag="P5236_05145"
     CDS             1108..2418
                     /locus_tag="P5236_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRU63782.1"
                     /translation="MIIDKELVDSLFAQFGTSDINQLSIREIGKLVSQIEKQSGIEFI
                     HMEMGVPGLPTLPVTVQAEKEALDKGLTSLYSDIEGIPFFKTETARFAKLFINLEVSP
                     LNCIPTVGAMQGSFAALLASARTNVKKPYTLFIDPGFPVNKSQLQLLGLPYKSFDILN
                     FRGEKLREKLLSYLKEGDICTITYSSPNNPSWINLTEEELQIIGELATQYDVIVVEDL
                     AYFGMDFRYDYGKPGVPPYQPTVAHYTDNYILLLSASKIFSYAGQRIGMMIISEKLRT
                     RHYPPLKNYFTSDVLGHFIPYGVLYATTAGTSHTAQYAVAATLKAINDGEVDFLKELA
                     VYGEKARQMKSLFLKNGFYIVYDEGGKLADGFYFTVAYPGMTSNELLKEFLYYGLSAI
                     SLTITGSSDPNGLRICVSFVRNDQIPELEKRLADFSKERTRKKN"
BASE COUNT          822 a          397 c          524 g          837 t
ORIGIN      
        1 cccactacgg taggttatac aaaaaatgat attcaaaact ggtgcgaatc acttgctcgc
       61 tgcttgacga tggcaggtgt gacaaaagaa gatgtggtgc aagtttctta tggatatggt
      121 ttgtttacag gtggcttggg tttgcactat ggagctgaaa caattggtgc tacggttatc
      181 ccagcttcag tagggaatac gaagcgtcaa ttacagttaa tgtctgatta tggtgttaca
      241 gtccttgcgt gtacaccttc gtatgctctt catctggtag atgcgatgtc ggaatttggt
      301 tattcacgtg ccgatatgaa attgcgattg ggtattttcg gagcagaacc atggactgaa
      361 aacatgcggc ttgaattgga aagaaaatgg ggaatccagg cttttgatat ttatggatta
      421 agtgaaataa tgggaccagg cattgctcat gattgtagtt ttcatcaagg attacatatc
      481 aatgaggatc atttttttcc agaaattgtc gacccgaaaa ccaaacagcc attaccagat
      541 ggttgtgaag gagaacttgt ttttaccaca ttgaccaaag aaggtattcc gcttttgcga
      601 tacaatacgc gcgatctttc tatgctcaat catgaaaaat gcgaatgtga acgtacgctg
      661 gttagaatga agaagattac tggcagaagt gatgatatgc tgattattcg gggtgtgaat
      721 ttatttcctt ctcaaattga acaagttttg ttggaaatgt gcgaaacgag tgcccattat
      781 cttttgtgtg tagatagaaa agataacctg gattatctgg aattgaaagt ggaattggat
      841 gaatcaaaaa tggtggagac tattcgtgaa ctggaaagtc tatcgaaaag aattgaacat
      901 tcccttcgca gtgctattgg tttaagcatc aaagttacac ttgttgagcc tcgttccatt
      961 ccgcgaagcg aaggaaaagc agtcagggta attgataacc gaaaaaagga agaaagcaaa
     1021 taattttttg atggaatata cgatttgata tttttgaaaa actcaattat tccgtaaaat
     1081 ataaagatat tattagaatt tttaatcatg attattgata aggaacttgt tgattcacta
     1141 tttgcccaat ttggtacttc tgatatcaat caactctcta ttcgtgaaat cggtaaactg
     1201 gtttctcaaa ttgaaaaaca aagcggtata gaatttattc acatggaaat gggtgtgccg
     1261 ggtttgccca ctttacctgt taccgttcag gcagaaaagg aagcattaga taaaggttta
     1321 acctcacttt attccgacat tgaaggaatt ccatttttta aaacggagac agcacgtttt
     1381 gctaaattat tcataaatct tgaagtcagt cctttaaatt gtattccaac agtaggagca
     1441 atgcaaggaa gttttgcagc tttattagca tctgctcgta ctaatgtaaa aaagccctat
     1501 acacttttca ttgatccggg ttttcctgtt aataagtcac aattacaact gttgggatta
     1561 ccttataaat cgtttgatat tttaaatttc agaggtgaga aacttcgaga gaaattgctt
     1621 tcctatttaa aagaagggga tatttgtaca attacttatt cgtctcctaa taatccctca
     1681 tggattaact tgacagaaga agaattgcaa atcattggtg aactggctac tcaatatgat
     1741 gtgatcgtgg tagaagatct ggcttacttt ggaatggatt ttcgttacga ttatggaaag
     1801 cctggtgtcc cgccttatca acccacagtg gcacattata cagataatta tattttgtta
     1861 ctttctgctt cgaaaatttt tagctatgct ggtcaacgaa taggaatgat gatcatttct
     1921 gaaaagttac gaactcgaca ttatccacct ttgaaaaatt attttaccag tgatgtgctg
     1981 ggacatttca ttccttatgg agttttgtat gcaacaactg ctggaacttc acatactgca
     2041 caatatgcgg tagctgctac cttgaaagca ataaacgatg gcgaggttga ttttttaaaa
     2101 gaacttgctg tttatggaga aaaagcccga caaatgaaat cattgttttt aaaaaatggt
     2161 ttttatatcg tttatgatga aggtggaaaa ttggcagatg gtttttattt tactgttgcc
     2221 tatcctggaa tgacgagcaa cgagttattg aaagaatttc tttattatgg tttaagtgcc
     2281 atttcgttga ccattacagg aagcagcgac ccaaatggtt tgcgaatttg tgtctccttt
     2341 gtaagaaatg accaaattcc tgaattagaa aaacgattag cagatttttc aaaagaaaga
     2401 acaagaaaaa aaaattgagt taatttatcc caaatttgtc attaggattg aatttctaat
     2461 ggcagtcatt agaaaaagta ctttataatt atttaaattt caatataatg tatcgacaaa
     2521 atattcaaaa aattgagact tttcaaaatt gaatgtaaga aatttttact caattttttt
//
LOCUS       DAOTKS010000232         2555 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_32915_length_2555_cov_6.230800, whole genome shotgun sequence.
ACCESSION   DAOTKS010000232 DAOTKS010000000
VERSION     DAOTKS010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32915_length_2555_cov_6.230800"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="P5236_05150"
     CDS             complement(<1..469)
                     /locus_tag="P5236_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HRU63783.1"
                     /translation="MPEIKTEGIKYVGSKLKIIPYILQILSELKEVKNVLDGFSGTTR
                     VSQAFAQSGYNTTSNDIAVWSEVFATCYLKSKGSDTYYQQLLDYLNSLKSYDGWFTEH
                     YGADEANSKRPFQRKNTQKLDAIRNEIDRLNLNWEDKCVLLTSLIYALDAVDNT"
     gene            complement(523..2394)
                     /locus_tag="P5236_05155"
     CDS             complement(523..2394)
                     /locus_tag="P5236_05155"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HRU63784.1"
                     /translation="MEEILEKDLLVDYGDSTIITLEGLEHVRRRPGMYIGKLGDGSHP
                     DDGIYVLLKEVLDNSIDEFRMGAGKTIEVQIQDGVVQVRDYGRGIPLGKMVEAVFIMN
                     TGGKYDSKAFKKSVGLNGVGMKAVNALSSTFIVQSFREGQSRRAEFSQGKLVRDSGIV
                     PDEGERGTLTIFVPDRTMFDGYVYKEEFVEAMLRNYAYLNTGLTLVFNGKKISSRNGL
                     SDLLNENLTSQPLYPLIHLIGEDIEIAFTHTNQYGEEYYSFVNGQHTTQGGTHQSAFR
                     EAVARTIKEFYSKNMELADVRNGIVAAIAISVEEPVFESQTKTKLGSRDMAPDGVSIN
                     KFISDFVKKELDNYLHRNPETADILFQKIQESEKERKAIAGITKIARERAKKVSLHNR
                     KLRDCRIHYNDTKGNTEESCIFITEGDSASGSITKSRDANTQAVFSLRGKPLNCYGLT
                     KKIVYENEEFNLLQAALNIEDGIDGLRYNKIILATDADVDGMHIRLLLITFFLQFFPE
                     LIKKGHLYILQTPLFRVRNKKETIYCYSEEEKKAAIARLGDQVEITRFKGLGEISPDE
                     FKNFIGEDMRLERVTLKKEDASAALLEFYMGKNTSERQNFIIENLKIEEENVEIEEM"
BASE COUNT          776 a          512 c          395 g          872 t
ORIGIN      
        1 tggtattatc aacggcatcc agtgcataaa taagacttgt tagtagaacg catttatctt
       61 cccaattcaa atttaatctg tcaatttcat tgcgaatggc gtctaatttt tgggtatttt
      121 ttcgctgaaa aggtcgtttg ctgttggctt catcagcccc ataatgttct gtaaaccagc
      181 catcatagct tttcaaacta tttaaataat ccaacagttg ctggtaataa gtatcgctac
      241 cttttgattt gagatagcag gtagcaaaaa cctctgacca aactgcaata tcattggaag
      301 tagtattgta acctgattga gcaaatgcct gagaaactct cgtagtacca ctgaagccat
      361 cgagtacatt ttttacctct tttaattcag aaagaatctg caaaatgtaa ggaatgattt
      421 ttaattttga accaacatat tttattcctt ctgttttgat ttcaggcatt tatttatcgc
      481 acaaattaaa taagaaaatt ttctacaaaa aggagaagag aattacattt cttctatttc
      541 cacattttcc tcctcaattt tcaggttttc aatgataaag ttttgacgtt cagaagtgtt
      601 tttccccata taaaattcca ataatgcagc agaagcatct tcctttttaa gtgttacccg
      661 ttccagccgc atatcttctc caatgaaatt tttaaattca tcaggagaaa tttcacctaa
      721 tcctttgaat cgcgttattt ctacttgatc acccaaacga gcaatagcag cttttttttc
      781 ttcttctgaa taacaataaa ttgtttcttt tttatttctt actctgaaaa gtggtgtttg
      841 aagaatatat aaatgtcctt ttttgatgag ttcaggaaaa aattgaagga aaaaagtaat
      901 caacagcagg cgaatatgca ttccatctac atccgcatca gtagcaagaa taattttatt
      961 gtaacgcagt ccatcaatgc catcttcaat atttaaagcc gcttgcaata gattaaattc
     1021 ttcattttca taaacaattt ttttggtaag tccataacag tttaatggct tgcctctcaa
     1081 gctgaaaaca gcctgcgtat tggcatctct gcttttggta atagaaccgc ttgccgaatc
     1141 gccttctgta ataaaaatac atgattcttc tgtatttcct tttgtatcgt tataatgaat
     1201 ccgacaatcg cgtaattttc gattatgcag gctgactttt tttgcccgtt cacgtgctat
     1261 tttggttatg ccggcaatgg ctttcctttc tttttctgat tcctgaattt tctggaataa
     1321 aatatctgcc gtttcaggat tacggtgcaa ataattatcc agttcctttt taacgaaatc
     1381 agaaataaat ttattgatag aaacaccatc tggtgccatg tcccgagagc caagcttggt
     1441 ttttgtttgc gattcgaata caggttcttc aacgcttatg gctatagctg ctacaatgcc
     1501 gtttcgaaca tcagccaact ccatattttt agaataaaat tccttgatgg tacgtgccac
     1561 tgcttcacga aaagcacttt gatgagttcc tccctgtgtg gtatgctgac catttacaaa
     1621 ggaatagtat tcttcaccat attgattggt atgtgtaaaa gcaatttcaa tatcttcgcc
     1681 tattaaatga atgagaggat aaaggggttg agacgtcaga ttttcattca gtaaatcact
     1741 caaaccatta cgggaagaaa tttttttccc gttaaaaaca agagtgagcc cagtattcag
     1801 ataagcatag tttcgcaaca tggcttctac aaattcttcc ttgtaaacat agccatcgaa
     1861 cattgttcga tcgggaacaa aaatcgtaag tgtcccacgt tcgccttcgt caggaacaat
     1921 tccgctatcc cttaccagtt tcccttgact gaattctgcc cgacgagact gaccttctct
     1981 aaagctttga acaataaaag tagaagaaag tgcgttaaca gctttcatgc cgacaccatt
     2041 caatccaacc gattttttaa aagcttttga atcatatttt ccacctgtat tcattataaa
     2101 aacagcttct accatttttc ccaaaggaat tccacgtcca taatcgcgta cttgcactac
     2161 accgtcttga atttggactt caatggtttt accagcgccc attctgaatt catcaatact
     2221 gttgtccaaa acctctttca gcagaacata aattccatca tcaggatgcg aaccatcacc
     2281 cagttttcca atatacattc ctggacgacg acgaacatgt tccaagcctt ccagcgtgat
     2341 aatggtgcta tcgccgtaat caaccaatag atctttttct aaaatttctt ccatattgat
     2401 taaaaaatca aaaacaatct cttgaatttt aaaaattttc tttaaaaatt attttattga
     2461 agctgatttt taaatttaag atgatagtaa ttcttgtttc agagcaaatc taactctgat
     2521 tatttgtaca aaaataagaa atttctggga tattt
//
LOCUS       DAOTKS010000233         2545 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_33093_length_2545_cov_3.531727, whole genome shotgun sequence.
ACCESSION   DAOTKS010000233 DAOTKS010000000
VERSION     DAOTKS010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33093_length_2545_cov_3.531727"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="P5236_05160"
     CDS             <1..981
                     /locus_tag="P5236_05160"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6359 domain-containing protein"
                     /protein_id="HRU63785.1"
                     /translation="TKAGTWEIKNFKVEEGEATTTPADTPTGEGTETSPYNVIAAIQN
                     QGNSKWVTGYIVGNIDGSGMSITTESKFTPPFTVKTNLLIADSPTETDYKKCLPVQLP
                     AGVVRDGLNLVDKANNLGKKVKLYGSLETYFGVPGLKGVSYFELEDGTSGGTKPSDTS
                     DVIFSETFANSKGDFTIVDVIKNPALSYIWTHDASYKCMKASAYANSTNYESESWLIS
                     PAINLSGKTNVTLNFEHTIGPASQLGIDKSHFTVWVSNNYSTGNPNEATWTKLDLSAS
                     SFNTTTPWTFVPVSLTIPSQYLTSNVRIAFKYTSTNTASATWEVKKVEVK"
     gene            1248..>2545
                     /locus_tag="P5236_05165"
     CDS             1248..>2545
                     /locus_tag="P5236_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRU63786.1"
                     /translation="MKKIFNLIFALFFPILFIQAQTSAGNGVITGKLIDKETKQPVAM
                     ANIRVLQQKDSTFVNGQASRNDGSFSIPVKYGAYIVHISFLGYKDIYKNVQVTSSRPI
                     VQLDTLYFEQTDIALSEIVIVERAPEIKVKGDTLEFNAGSFKVTESAVVEDLLKKMPG
                     VEIDKDGKITVNGREIKKILIDGEEFFSNDPKVASKNLPAKMVEKVQVLDKKSEMAEM
                     TGFDDSDEETVINLIIKQGMKEGLMGNAFAGYGSKDRYEGNAMVNYMKNKNQYTFIGG
                     LNNTNNAGFTDLASSMFGNIGGRGLRGMFGNQDGITTSGMGGFNFNTQLSKTFKLGGN
                     IRYGSTDNNVLSKVHTQNLIKDNNTFENENDTTNNYSQNFNMDLRMEWTPDTLTRIIF
                     RPSAGFYQNHRSETSDFFTTNELNDTINNGNSDYYSEGNG"
BASE COUNT          921 a          402 c          469 g          753 t
ORIGIN      
        1 acaaaagccg gcacgtggga aataaaaaat ttcaaagttg aagaaggtga agctaccact
       61 acacctgccg atactcccac aggtgaagga acggaaacct ctccttataa tgtaattgct
      121 gccattcaaa atcaaggtaa ctcaaaatgg gtgacaggtt atattgtagg taatattgac
      181 ggtagtggga tgagtataac aactgaatca aaattcacac ctccttttac tgttaaaaca
      241 aatttgttga ttgctgattc cccaactgaa acagattata aaaaatgttt acctgttcaa
      301 ttaccagcag gagtcgtcag agatggttta aatttggtag acaaagctaa taatttaggg
      361 aaaaaagtaa aattgtatgg gtctttggaa acttattttg gagtgcctgg tttaaaagga
      421 gtttcttatt ttgagcttga agatggcacc agtggtggca caaaaccgtc agacacttca
      481 gatgttattt ttagcgaaac atttgcaaat tcaaaaggag attttacaat agtagatgtt
      541 attaaaaatc ccgctttgtc ttatatttgg actcacgatg cttcatacaa atgtatgaaa
      601 gcttctgctt atgccaactc taccaattac gaatcagaaa gctggctcat ttctcctgct
      661 atcaatttat cagggaaaac gaatgttaca ttaaattttg aacatactat cggtccagcc
      721 tcacaattag gtattgataa atcacacttc accgtatggg ttagtaacaa ctattctaca
      781 ggaaatccaa atgaagctac ttggacaaaa cttgatttat cagcaagttc atttaatact
      841 acaacacctt ggacttttgt accggtaagt ttaacaattc cttcacaata tttaacttca
      901 aatgtaagga ttgcttttaa atatacgagt acaaacacgg cttcagcaac atgggaagta
      961 aaaaaggtag aggttaaata agtgggaaag tcaggataat ttgattatca ataaaataac
     1021 tcacattttc tcatctaagt gcaaagtttt tgagagaaaa attgctcaaa actttgcact
     1081 tatttatttc tgactcccat ttggtattaa atcaattaaa aaaagatttg tcattgcaaa
     1141 aatcctattg gtcgagaaaa aaacttcatt tgtctatttt atttgtgaat gtagaaaaac
     1201 agaaataatt ttgaaacaaa aaagttgaaa tgattaacaa gtaaaaaatg aaaaaaatat
     1261 ttaatcttat ctttgcccta tttttcccca ttttgtttat ccaagcacaa acatccgctg
     1321 ggaatggtgt gattacagga aaattgattg acaaagaaac taaacaacca gtggctatgg
     1381 ccaatataag ggttcttcag caaaaagaca gtacttttgt caatggacag gcaagtagaa
     1441 acgatggttc tttttccatc cctgtcaaat atggtgccta tattgttcat atttcatttt
     1501 taggatacaa agatatttat aaaaacgttc aagttacctc ttcacgtcca attgttcaat
     1561 tagatactct ttattttgaa cagacagata ttgccctttc cgaaatagtt attgtggaaa
     1621 gagcccccga aataaaagtc aaaggtgata cacttgaatt taatgctggc tcgttcaaag
     1681 taactgaaag cgcagtagta gaagatctct taaaaaaaat gcctggtgta gaaatagata
     1741 aagacgggaa aattacagta aacggtcgtg aaattaaaaa aatattaatc gatggcgaag
     1801 agtttttcag taatgatcca aaagtagctt ctaaaaacct tcctgcgaaa atggtagaaa
     1861 aagtgcaggt attagataaa aaatctgaaa tggcagaaat gactggattt gatgatagtg
     1921 acgaagaaac tgttattaat cttataatca agcaaggaat gaaagaagga ctaatgggga
     1981 atgcttttgc aggctatgga agcaaagacc gttatgaagg aaatgctatg gtaaattata
     2041 tgaagaacaa aaatcaatat actttcattg gcggtctgaa caacacaaat aatgcaggat
     2101 ttactgattt ggcttcgagc atgtttggaa acataggagg acgcggttta agaggaatgt
     2161 ttgggaatca ggatggaatt acaacgtcgg gtatgggtgg atttaatttc aatacacagc
     2221 tttccaaaac tttcaaactt ggtggaaata tccgttatgg ctctaccgat aataatgttt
     2281 tgtcaaaagt tcatacccag aatttaataa aagacaacaa tacgtttgaa aatgagaatg
     2341 atactaccaa caattacagc caaaatttca atatggattt gcggatggaa tggacacccg
     2401 atacacttac acgcatcatt tttcgtccca gtgcaggttt ttaccagaac catcgcagtg
     2461 aaacaagtga tttttttact accaatgaat taaatgatac tatcaataac ggtaattcgg
     2521 attattattc agaaggaaat ggtaa
//
LOCUS       DAOTKS010000234         2542 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_33141_length_2542_cov_2.809007, whole genome shotgun sequence.
ACCESSION   DAOTKS010000234 DAOTKS010000000
VERSION     DAOTKS010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33141_length_2542_cov_2.809007"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..1634)
                     /locus_tag="P5236_05170"
     CDS             complement(36..1634)
                     /locus_tag="P5236_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRU63787.1"
                     /translation="MKRHYLTIFILSILFIFNSFQSNGQGISLIQQRKLINALNIISN
                     MYVDSVNDKKLVETAIVSMLKELDPHSVYIPKEEVERINEPLEGSFEGIGIQFQMFQD
                     TLLVVQTIAGCPAEKVGIRPGDRIIYVDDKLIAGVKMQNSDIMKLLRGPRGTEVTVKV
                     LRREEPELLIFKIVRDKIPLYSVDANYLVRKDIGYIKINNFGNSTAAEFRKALTELQK
                     KGMKHLILSLQGNGGGYLGTAIQLADEFLEQGKLIVYTQGLRQPKKEWLATSHGDFEK
                     GKLIVLVDEYSASASEIVAGALQDWDRAIIIGRRTFGKGLVQQQIPLNDGSMLRLTTA
                     RYYTPTGRCIQKPYKDGIDKYEQELLNRHKKGELLHADSVHFPDSLRYQTLILHRTVY
                     GGGGIMPDIFVPLDTTRYTDYHQKLVARGVLNKVYIQYIDQHRNELTKNYSSFEKYKN
                     DFEVNNVLLQQLINEGEKEQIPFNEAQFNTSKELIKLQLKALIARDLWDVNAYYQIMD
                     VENSSLQKAIEILETPGAYEKILQ"
     gene            complement(1670..2335)
                     /locus_tag="P5236_05175"
     CDS             complement(1670..2335)
                     /locus_tag="P5236_05175"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRU63788.1"
                     /translation="MTDSMEIFVDIHTHQLENQNYYSIINFDLQQAEKFLSTDTSFFC
                     SIGIHPWKVASITKEEVFQLKKWENDSRWIAVGECGLDKNSKASLTLQQYFFEKQITL
                     SEKLKKPLIIHCVKCFNEIIEIKKNLNPNQKWIIHGFRGKPQLAIQLLNNNIGISYGE
                     KFNPQSVEITPFEQLYVETDESKLPISTIYQQIALVKHCAVTKLNAGKKLLDLKENNN
                     LLI"
BASE COUNT          804 a          449 c          371 g          918 t
ORIGIN      
        1 ataactaaat ccataagaat ttttagttta gaattttatt gcagaatttt ttcataagct
       61 cctggagttt caagaatttc aatggctttt tgtaaagatg aattttccac atccataatt
      121 tgataatagg cattcacgtc ccataaatcg cgagcaatta aagcttttaa ttgtaatttt
      181 atcaattctt ttgaagtatt aaattgtgct tcattgaaag gaatttgctc tttttctcct
      241 tcatttatca gttgttgtaa caaaacgtta ttaacttcaa aatcattttt atatttttca
      301 aaagagctgt aattttttgt cagttcattt ctatgttgat caatgtactg aatatatact
      361 ttatttaaaa cgccgcgtgc tactaatttt tgatgataat ctgtataacg ggtagtatcg
      421 aggggtacaa aaatgtcagg cataattcca ccacctccat aaacagttcg atgaagaatt
      481 aacgtctgat aacgcaatga atctggaaaa tgaacactat ctgcatgcaa tagttctcct
      541 tttttgtggc ggttcaataa ttcctgttca tatttatcaa ttccatcttt ataaggtttt
      601 tgaatgcaac gtcctgttgg agtataataa cgagcagtag taagtcgtag catcgaaccg
      661 tcgtttaaag gaatttgttg ttgaaccagt ccttttccga aggttcgtct tccaataatg
      721 atggctctgt cccagtcttg caaagctcct gctacaattt cgctggcaga agcggaatat
      781 tcatctacca acacgattaa tttacctttt tcaaaatcac cgtgagaagt agcaagccat
      841 tcttttttcg gctgtcgcaa accttgtgta taaacaatta atttcccttg ctctaagaat
      901 tcatcagcca attggatggc agttcccaaa taaccgccac catttccctg taaactcaaa
      961 ataagatgct tcattccttt tttctggagt tcagttaaag ctttacgaaa ttcggcagca
     1021 gtagaatttc caaaattgtt gattttaatg taaccaatat cttttctcac cagataattt
     1081 gcatccacac tataaagagg aattttatct cgaacaattt taaatattaa cagttcaggt
     1141 tcttctctcc ttaaaacctt tactgtcact tcggtgcccc gtggtccccg caaaagtttc
     1201 attatatcgg aattttgcat ttttaccccg gcaatcaatt tatcatccac gtaaataatg
     1261 cgatcacctg gacgaatgcc aaccttttca gccgggcagc cagcaatggt ttgaacgacc
     1321 agcagcgtat cctgaaacat ttgaaattga ataccaattc cttcaaaact tccttccagc
     1381 ggttcattaa tgcgttccac ttcttcttta gggatataaa ctgaatgagg atccaattct
     1441 ttcaacattg aaacaatagc agtttcaacc aattttttat cgttcacact gtccacatac
     1501 atattggaaa taatattcaa ggcattgatc aatttgcgtt gctgaatcaa tgaaattcct
     1561 tgcccattac tttgaaaact attgaaaatg aatagaatgc ttaaaataaa tattgtcaaa
     1621 taatgtctct tcataaaatt ttgtatattg taaaatttgt tatgtttttc taaatcaata
     1681 aattattatt ttcttttaaa tctaataatt ttttccctgc atttaatttt gtcactgcac
     1741 aatgttttac caaagctatt tgctgataaa ttgttgaaat gggtaatttg ctttcatctg
     1801 tttctacata aagttgctca aaaggcgtta tttctacact ttggggatta aatttttcgc
     1861 cataagaaat acctatatta ttatttaaca attgaattgc cagttgaggt tttcctctga
     1921 atccatgaat gatccatttt tgattgggat tgagattttt ctttatttcg ataatttcat
     1981 taaaacattt tacacaatga ataatcagcg gttttttcaa tttttctgat aatgttattt
     2041 gtttttcaaa gaaatattgt tgcaatgtca atgaagcttt tgaattttta tccagtccac
     2101 attctccaac ggcaatccaa cgagaatcat tttcccattt tttcagttga aaaacttctt
     2161 cttttgtgat agaagcaacc ttccagggat gaatgccaat tgaacaaaaa aaggatgtat
     2221 ctgtcgatag aaatttttct gcctgttgta aatcaaaatt aatgatcgaa taataattct
     2281 gattttctaa ctgatgcgta tgaatatcaa caaaaatctc cattgaatcc gtcattattt
     2341 aaaaggaaac atttgacaaa agtagctatt tttgaataaa aacattaact ttgcacgcaa
     2401 ttatttagta ataaagtttt tcagaaagaa tcgaaccatt caaaaaataa caaaatgagt
     2461 ttttcacaat tattcagttc attaccttat aaagtagctg atattacttt agctgatttt
     2521 ggacggaaag aaatagaatt gg
//
LOCUS       DAOTKS010000235         2538 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_33211_length_2538_cov_4.668546, whole genome shotgun sequence.
ACCESSION   DAOTKS010000235 DAOTKS010000000
VERSION     DAOTKS010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33211_length_2538_cov_4.668546"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="P5236_05180"
     CDS             complement(<1..553)
                     /locus_tag="P5236_05180"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HRU63789.1"
                     /translation="MKTSFLNYLVIITIFILGGCSNVDEPEEPTSPAQSLPFIETFEQ
                     GFGKFTTQNVLGDQVWEYSPQYKYVMITGYVDADGDGTKENHANEDWLISPEIDLSNV
                     TAAKLTFEHVIRYFDNPATEATLWVSENYIDGLPSTATWTQLTTPLFTNFSDWTFGSS
                     GEISLTPYAGKKIRIAFKYISTDT"
     gene            complement(584..1522)
                     /locus_tag="P5236_05185"
     CDS             complement(584..1522)
                     /locus_tag="P5236_05185"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HRU63790.1"
                     /translation="MKKVKYIYFVLAIFLGSCAKTEFDGIPFEKTQGEKWETTITIAQ
                     LKNNYMTSTGLFTADKIENTTDLVFNGIVTSSDVEGNVYKYLTVQEEGENAQAIKISI
                     DVSGLSAIYPLGQRISVRCNDLYIGKYAESPQIGIRYVNKEKNNRIEPGRMPKLLSDI
                     HISAYELPEPQAVIADTMTIAEIKAADSSLFNKLVCIKNAFFTGKGANYGKPATIPDN
                     DKIFAPPTNGIGYPQSREIQDGTGSIFVSTSEYAKFASKPLPPSTMKGNITAIVGWYN
                     DKDDPYDSSKIYHQLTLRSLKDLGKGFESYLAQYNN"
     gene            complement(1588..>2538)
                     /locus_tag="P5236_05190"
     CDS             complement(1588..>2538)
                     /locus_tag="P5236_05190"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRU63791.1"
                     /translation="KVWWFVDPSLKAGITYKIDGRNRLSINGIAETRAPLASKSYISE
                     RIKDTLLPHLKSEKVWSADINYNFSFPVIRGRIGLFETNVYGTSDKLGYYDDEYRTFI
                     NHVLADVNKVYKGMEAGASIKLNSNFSVAIAGTYADYSYTNNAIGIKSPENGSFADIS
                     ETVLTKGLKIANGPQLAANITLDYFHPKMWFADITLNYFDNNYLDFAPNRFTENNMAK
                     YTTPEMLAALGTQEKLKNGFLLDASIGKLIYLKNRRSLNFNLSASNILNNQKMITGGY
                     QQARLPLTDGAIDKNGLDRFPNKYYYAWGFNLFFHIGYKF"
BASE COUNT          782 a          429 c          428 g          899 t
ORIGIN      
        1 ttgtatcggt agaaatatat ttgaaagcaa ttctgatttt tttcccagca taaggtgtca
       61 aacttatttc tccagagcta ccaaatgtcc aatcactgaa attggtaaaa agtggtgtag
      121 tcagttgagt ccatgtagct gtggaaggta agccgtctat atagttttct gaaacccata
      181 aggtagcttc tgttgcagga ttatcaaaat agcgaattac atgttcaaag gttagttttg
      241 ccgcagttac attggacaga tcaatttcag gagagattaa ccaatcttca tttgcatgat
      301 tttcttttgt tccatctcca tcggcatcga cataaccagt aatcataacg tatttgtatt
      361 gaggagaata ttcccaaact tggtcgccta acacattttg tgtcgtgaat tttccaaaac
      421 cttgttcaaa agtttcaata aaaggaaggg attgtgctgg agaggtaggt tcttccggtt
      481 catctacgtt ggaacatcct cctaaaataa aaatagtaat aatcactaaa taatttaaaa
      541 aacttgtttt cataatagat agaaatttga taatgtattg atcttaattg ttatattgtg
      601 ctaagtaaga ttcaaatcct tttcctaagt ctttgagtga acgtaaagtt agttgatgat
      661 aaattttcga tgaatcatat ggatcgtctt tatcgttgta ccagcctaca atagcggtaa
      721 tgtttccttt catggttgaa ggaggtaatg gctttgatgc gaatttagca tattcactgg
      781 ttgaaacaaa aatcgagcca gttccatctt gaatttctct tgattgtgga taaccaatac
      841 cattcgttgg tggagcaaaa atcttatcat tgtcaggtat tgtagctggt tttccataat
      901 tagctccttt accggtaaaa aaggcatttt tgatacatac aagtttatta aataaggagg
      961 agtctgctgc ttttatttcg gcaattgtca tagtgtcggc tatgacagct tgtggttcgg
     1021 gtaattcata agcagagata tgaatatcgg acaataattt tggcattcgt ccaggttcaa
     1081 ttctattgtt tttttctttg tttacgtatc taataccaat ttgaggagat tcagcatatt
     1141 tgccaatata caaatcatta caacgaacag aaattctttg ccccaaagga tatatagctg
     1201 ataaaccact aacatcgata gagattttta tagcctgtgc attttctcct tcttcctgca
     1261 ccgtcagata tttatacaca ttaccttcta catcagaaga agtaacaatc ccattaaaaa
     1321 cgagatcggt agtattttca attttatctg ctgtaaataa ccctgtagat gtcatataat
     1381 tatttttcaa ctgagcaata gtgatggtgg tttcccactt ttcgccttga gttttttcaa
     1441 atggaattcc gtcaaattcg gtttttgcac aacttcctaa aaatatggca agaacaaaat
     1501 aaatatattt tacttttttc atttttccta attattaatt ttttttagac aattatttta
     1561 tatttatcaa atgattttat tgatctttta aaacttatat ccaatatgga agaacaaatt
     1621 gaatccccag gcataataat atttattagg aaatctgtca agaccattct tatcaattgc
     1681 tccatctgtc aatggtaagc gagcttgttg ataaccacct gtaatcattt tttgattatt
     1741 caaaatatta ctggcactta aattaaaatt taaggagcgt ctgttcttta gataaattaa
     1801 tttcccaatt gatgcatcca agaggaaacc atttttaagt ttttcttgag tgcccagtgc
     1861 tgctaacatt tctggtgtag tatattttgc catattgttt tcagtaaacc tgttaggagc
     1921 aaagtccaaa tagttgttgt caaagtaatt caaagtaata tctgcaaacc acatttttgg
     1981 gtggaaatag tctaatgtaa tgttagctgc taattgaggt ccattggcaa tttttaatcc
     2041 ttttgttaaa actgtttcag aaatatcagc gaacgaacca ttttcaggac ttttaattcc
     2101 tattgcattg ttggtataag agtaatcagc ataagtgcct gctattgcta cagaaaaatt
     2161 actattcaat tttattgatg ctccagcttc cataccttta taaaccttat ttacatctgc
     2221 caaaacatga ttaataaagg ttcggtattc atcgtcgtaa tagcccaact tatcggaggt
     2281 gccataaacg ttcgtttcaa ataagccaat tcttcctctg atcacaggaa aactgaagtt
     2341 gtaattgatg tcagcagacc aaactttttc agattttaaa tgtgggagca aggtatcttt
     2401 gattcgttct gaaatataag atttgcttgc cagcggagct cttgtttcgg caattccatt
     2461 gatggacaat ctgtttcgtc catcgatttt ataagtaata ccagctttca aggaaggatc
     2521 tacaaaccac caaacttt
//
LOCUS       DAOTKS010000236         2530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_33364_length_2530_cov_5.669091, whole genome shotgun sequence.
ACCESSION   DAOTKS010000236 DAOTKS010000000
VERSION     DAOTKS010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33364_length_2530_cov_5.669091"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="P5236_05195"
     CDS             complement(<1..966)
                     /locus_tag="P5236_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63792.1"
                     /translation="MKKNIGVWVLLLCSNLIVAQSELDIVKFVQPDINGTARYMSMAG
                     AFGALGGDVSAIKDNPAGLGIYQHSETVGTLNFLFQNNDNSWYGVNSSDNLYKLKANN
                     FSFVIASPTLQSKKGIKSGLQNSNFSFSFQQVKNFNRQMTIQSGESPSSITDYMAYFT
                     NGILEADLQSTDDYEPFDNQNIPWLSILAYEGYLINPASNNSWVSILNADEKVIPSYY
                     LHESGHINKYSFSWAGNFSHMFYLGFTGNIQTIDYNAVTEYEETFGEGGGMNLNNTID
                     ASGSGFNLEMGIIFRPVDNFRIGLSFQSPTVFSISEDYTSLLNFNT"
     gene            complement(1010..2299)
                     /locus_tag="P5236_05200"
     CDS             complement(1010..2299)
                     /locus_tag="P5236_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63793.1"
                     /translation="MKPIQLIIIFLSLLIPTLGYSQKETKVIDDIYYSPNDKTPTYSS
                     KNEQKPTYKNGAKEIIFREHSDSIKENSTLSMTKEENDSLLNDTIHEEGYYLHGFKGS
                     SSDYEYAERIRRFHNPKFTIHISDPDYMDLYFLDDYNWNVYIEGGYAWVTPTWTNPLW
                     FDYYYRPFMYNSWYWRWSLYYPSYSYWYSPWYYYPWHAWYYDPWYYSPWYYGYYRHPY
                     YGGYYHYKTINPSHNEANRRQITNFGNERRVANSPSSAMSTNSDNVSQRNGRSNNPYT
                     VITGTGARNSNTTGMTTPSNAGNSSSEQTRRGAVLNNQNQSERTYNSANTRSSSSSYA
                     NSENSRRSTSVNTQPRTSNTTNNSSGVRANTRSTYSAPSRSSSGESNRSGSSYSSGSS
                     SGSSTRSSGSSNSSGGSRSSGGSSSGSSRNSSGGGRR"
BASE COUNT          815 a          452 c          420 g          843 t
ORIGIN      
        1 agtgttgaaa tttaagagag atgtataatc ttctgatata gaaaaaactg tagggctctg
       61 gaaggagaga ccgattctga aattgtcaac aggtcgaaaa ataataccca tctctaaatt
      121 gaaaccgctc cccgacgcat caatagtgtt gttcagattc atacccccac cttcaccaaa
      181 agtttcttca tattcagtaa cagcattata atcaatagtc tgaatattac cggtaaaacc
      241 caaataaaac atatgactga aatttcctgc ccaactgaaa gaatatttat taatatgacc
      301 actttcatgc aaatagtaag aaggaatgac tttctcatct gcatttaata ttgaaaccca
      361 tgaattattg gaagcaggat taattaaata tccttcgtaa gctaatatgg ataaccaggg
      421 gatattttga ttatcaaaag gttcataatc atcagtactt tgtaaatctg cttcaagaat
      481 tccattagta aaataagcca tataatcggt tattgatgaa ggtgactctc cgctttgaat
      541 agtcatctgg cgattaaaat ttttcacctg ttgaaaagaa aaagaaaaat tagaattttg
      601 taatccactt ttgattcctt ttttactttg taacgtgggt gaagctatca cgaatgaaaa
      661 attatttgct tttaatttat aaagattatc acttgaattt actccatacc agctattatc
      721 gttattttga aataagaaat ttaaagttcc aactgtctca gaatgttgat aaatacctaa
      781 accagctgga ttatctttaa ttgctgaaac atctccccct aacgctccaa atgctccagc
      841 catgctcatg tatcgagccg taccatttat atcaggttga acaaatttta ctatatccaa
      901 ttcactttga gcaacaatca aattactgca aagcaataac acccataccc caatattttt
      961 cttcatgttt taataaattg attaataatt cattaattgt tataaaattt taccgtcttc
     1021 cgccaccact tgaatttcta cttgacccac tcgaactacc tccactgctc cggcttcctc
     1081 cactactatt agaagaacct gaagagcgag ttgaacttcc gctcgaactt cccgatgagt
     1141 agctggagcc acttcggttg ctttcaccag atgagcttct gcttggagca gaataagtcg
     1201 atctggtatt tgctctcact cctgaagaat tgtttgtagt atttgaagta cgaggctgag
     1261 tgtttacaga agttgatctt ctggaatttt cactattcgc ataagaactt gacgaagaac
     1321 gagtattagc agaattatat gttctttcag attgattttg attatttaag acagcacctc
     1381 ttcttgtttg ttcagaactt gaattaccag cattacttgg ggttgtcatt cctgttgtat
     1441 tactgtttcg tgctcctgtt cctgtaataa ccgtataggg attattagaa cgtccatttc
     1501 tttggctaac attgtctgaa ttggtagaca ttgcactaga aggagaatta gctactcttc
     1561 tttcatttcc aaaattggta atttgtcggc ggttggcttc attatgagaa ggattaatcg
     1621 ttttgtaatg ataatatcct ccataataag gatgtctata atagccataa taccatgggg
     1681 aataatacca agggtcataa taccacgcat gccatggata gtaataccac ggactatacc
     1741 aatatgaata agaaggataa taaaggctcc atcgccaata ccagctattg tacataaaag
     1801 gtcgataata gtaatcaaac cagaggggat tggtccaggt tggtgttacc cacgcataac
     1861 ctccttcgat ataaacattc cagttataat catcaagaaa atacaaatcc atgtaatcag
     1921 gatcggaaat atgaatagta aattttggat tatggaatct acgaatacgt tcagcatatt
     1981 catagtcgga agaggaacct ttaaaaccat gcaaataata gccttcttca tgtattgtat
     2041 cgttgaggag gctgtcattt tcctccttag tcatcgaaag agtactattt tctttgatgg
     2101 aatcgctatg ttctctaaaa atgatctcct ttgcaccatt tttgtaagtg ggtttttgtt
     2161 catttttaga agaatatgtt ggtgttttgt cattaggcga ataataaata tcgtctatca
     2221 cctttgtttc tttctggctg tatcctaatg ttgggataag cagagaaaga aaaatgataa
     2281 ttaattgaat aggtttcatg atattttttt gtttaaaaat aggtttttca tcctaaataa
     2341 tacaacaatc taaataatac aacaattatg ccattgtttt gtttttcata ggttataaaa
     2401 gttgaattcg atgttactta aaaaaaaata atttttaatg attatccgca tgaattccta
     2461 ttgtaaattc ttgacctgaa atcggtgttg tagcttacag cagcaacaag caacctgtcg
     2521 aagattgcat
//
LOCUS       DAOTKS010000237         2528 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_33403_length_2528_cov_4.587545, whole genome shotgun sequence.
ACCESSION   DAOTKS010000237 DAOTKS010000000
VERSION     DAOTKS010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33403_length_2528_cov_4.587545"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..1191
                     /locus_tag="P5236_05205"
     CDS             79..1191
                     /locus_tag="P5236_05205"
                     /inference="COORDINATES: protein motif:HMM:NF016830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HRU63794.1"
                     /translation="MEWHRVRKTLLIGIILLHNVIKLAAQEKNETSFFTFSASYIGDG
                     FANMSGGIKTGISYLGLISLNTYFDTSKAHLWKDGGLFLNIFNTHGGQPTAHFVGDYQ
                     GVSNIEASNHTYLYELWYEQKFKKVKSIIGLQDLNVNYAASEYGSLFINSSFGIPSTI
                     AYNIPSPIFPLTALGFHLEWQPTQHFKWKAAIFDGTPEGFENFHLKWKLNKQNGFLNI
                     TEMEWNKNFQENRSGIYKIGAYYHNSRDSMQHTEENRGFYFVGDQTVNKHVGIFSQIA
                     ASPASKNSNPFYLGIGFNYKGIGKKRQEDIFGLALAYASLKNRRDEATIEMIYQFVIN
                     RHISLMPDIQYVINSSGTDIALKNSLISFLRMRLDF"
     gene            1204..>2528
                     /locus_tag="P5236_05210"
     CDS             1204..>2528
                     /locus_tag="P5236_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused ferrous iron transport protein A/B"
                     /protein_id="HRU63795.1"
                     /translation="MYEINNFTRLSDLSPGDEAIIVKVLGHGSFRRRLAEMGFVKGKK
                     VKVIKCAPLQDPIEYEIMGYNISLRRSEAELIQVISVQEATSVLQNVHFNGIIDDETL
                     KVSALEKGKSIHVALVGNPNCGKTTLFNRISGAHERVGNYGGVTVEIKEAEVKKNGYT
                     IKLVDLPGTYSITEFSPEELYVRVYLTEKMPDIVLNVIDASNLERNLFLTTQLIDMDL
                     KVVIALNMYDELEKKGDKLDYKALGKMLGIPIVPTVATKGEGIEELLQKIIDVYEDKD
                     PIVRHIHINYGSVIEKAIKQIQDVIWQNPIITDKLSSRYLAIKLLEGDKAILSELEKY
                     ENYPQIKSITKQAIQNVEKEYGDTCDTVITQLKYGFIEGALKETYHLSNQVNKIKQQT
                     LDNLFLHNFWGYPIFLFFILLMFQATFSLGKYPTEWLESGINLLSGWVE"
BASE COUNT          876 a          365 c          468 g          819 t
ORIGIN      
        1 ttgtttagaa tcaatctaaa taactatctt tgtcatcgca aatcaaatct taggaatgat
       61 taaaattata aagaaaaaat ggaatggcat cgagtgcgaa aaactctttt gataggaata
      121 attcttttgc ataatgttat aaaattagct gctcaagaaa agaatgaaac ttcatttttt
      181 actttttcag cttcgtatat tggtgatggt tttgcaaata tgtcaggtgg aataaaaaca
      241 ggtatttctt atttgggatt aatttcatta aatacctatt ttgatacttc aaaagcacat
      301 ttatggaaag atgggggctt atttctgaat atattcaaca ctcatggtgg acagcctacg
      361 gctcattttg ttggtgatta tcaaggcgtt tcaaacatcg aagcaagtaa tcatacttat
      421 ttgtacgagc tctggtatga acaaaaattt aaaaaagtga aaagcatcat tggtttacag
      481 gatttgaatg tgaattatgc tgccagtgaa tatggaagtt tatttatcaa tagttcattc
      541 ggaattcctt ccaccattgc ttataacatt ccttctccta tttttccgtt aacagcattg
      601 ggctttcatt tagaatggca acccacccaa catttcaaat ggaaagcggc aattttcgac
      661 ggtacgccag aaggttttga aaattttcat ttgaaatgga aattaaacaa gcaaaatggc
      721 ttcttaaaca ttacagaaat ggaatggaat aaaaacttcc aggaaaatcg ttcaggaatt
      781 tacaaaattg gagcttatta tcataacagt cgtgatagca tgcaacatac agaggagaat
      841 agaggttttt attttgttgg tgatcaaact gtcaataaac atgttggaat attttctcaa
      901 attgcagcga gtcctgcatc aaaaaattca aatccctttt atttgggtat agggtttaat
      961 tataaaggaa ttgggaagaa aagacaagaa gatatttttg gtttagcttt agcttatgca
     1021 tcgctaaaaa atagaagaga cgaagcaact atagaaatga tatatcaatt tgtcatcaat
     1081 agacatatca gtttaatgcc agatattcag tatgtaatca attcttcagg aactgatata
     1141 gcattaaaaa attcgctgat aagttttctg agaatgcgat tagactttta aaattggaat
     1201 atagtgtatg aaataaataa ttttacacga ctttctgacc tatcgcctgg tgatgaggca
     1261 attatagtaa aagtgctggg acatggttct tttcgcagaa gactcgctga aatgggcttt
     1321 gtaaaaggta aaaaagtaaa ggttattaaa tgtgctccat tacaagaccc catagaatat
     1381 gaaattatgg gatataacat ttcgttgcgg cgaagtgaag ctgaattaat ccaggtaatt
     1441 tctgttcagg aagcaaccag tgttttacaa aatgtacatt ttaatggaat tattgacgat
     1501 gaaactttaa aagtatcagc tcttgaaaaa ggaaaatcta ttcatgttgc tttggtgggt
     1561 aatcccaatt gtggaaaaac tactttgttc aatcgcattt ctggagctca cgagcgagta
     1621 ggaaattatg gtggtgtcac tgtagaaata aaggaagccg aagttaaaaa aaatggctat
     1681 actattaagc tggttgattt gcctggtact tattctatca cagaattttc tccagaagaa
     1741 ctttatgttc gcgtttattt aaccgaaaaa atgcctgata tcgtactcaa tgtgatagat
     1801 gcttccaatt tggaacgtaa tttatttctg actactcagc tcattgacat ggatttgaag
     1861 gttgtgattg ccttgaatat gtatgatgaa ttagagaaaa aaggagataa acttgattac
     1921 aaagctttag gaaaaatgct gggaataccc attgtcccaa ctgttgccac caaaggtgaa
     1981 ggtattgaag aacttcttca aaaaataata gatgtttacg aagataaaga tcccattgtt
     2041 aggcatattc atattaatta tggttctgta attgagaaag ctatcaaaca aattcaggat
     2101 gtaatctggc aaaatcctat tataactgat aaattatctt cccgttatct ggctatcaaa
     2161 ttgttggaag gtgataaagc cattctctca gaactagaaa aatatgagaa ttatcctcaa
     2221 atcaaatcta ttacgaagca agctattcaa aatgtggaaa aagaatatgg tgacacttgc
     2281 gatactgtta ttacccaact aaaatatggt tttattgaag gggctttaaa agaaacttat
     2341 catctttcta atcaagtcaa taaaataaag caacaaactt tagacaattt gtttcttcat
     2401 aatttctggg gatatcctat ctttctgttt ttcattttgt taatgtttca ggctactttc
     2461 agcctgggaa aatatccgac cgaatggctg gaaagtggaa ttaatttact gtctggatgg
     2521 gttgaaaa
//
LOCUS       DAOTKS010000238         2526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_33437_length_2526_cov_4.164711, whole genome shotgun sequence.
ACCESSION   DAOTKS010000238 DAOTKS010000000
VERSION     DAOTKS010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33437_length_2526_cov_4.164711"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2028
                     /gene="ppdK"
                     /locus_tag="P5236_05215"
     CDS             <1..2028
                     /gene="ppdK"
                     /locus_tag="P5236_05215"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HRU63796.1"
                     /translation="ILYRKMEGIPDDWGTAVTVQAMVFGNMGDTSATGVAFTRNAATG
                     ENVFNGEYLVNAQGEDVVAGIRTPLQITKAGSQQWAKLAGISEEERRTKYPSMEEALP
                     DIYKELNEIQQKLENHYKDMQDIEFTVQEGKLWLLQTRSGKRTGTAMVKIAIDMLHEG
                     IIDEKTALLRIDPLKLDELLHPIFDKESLKNVKVIAKGLPASPGAASGKIVFNADDAA
                     EWAKQGEKVIMVRIETSPEDLAGMAAAQGILTARGGMTSHAAVVARGMGKCCVSGAGA
                     LKIDYVNRTIQINDLVLKEGDYISINGSTGEVYLGKVPTKEPELDPYFIELLTLADKY
                     ADLRVRTNADTPRDASVARKFGATGIGLCRTEHMFFEGNKIKAMREMILAEDTEGRKK
                     ALAKLLPYQRADFEGIFEVMEGLPVTIRLLDPPLHEFVPHDEKGQQEMAEAMGVPLKK
                     IQERVESLREQNPMLGHRGCRLGNTFPEITEMQTRAILEAVLNLKAKGIKIIPEIMVP
                     LVSVKKELVEQERIIRETANKIFEEKGDTIEYKVGTMIEVPRAALTANEIAENADFFS
                     FGTNDLTQMTFGFSRDDIGSFLPIYLEKKIIESDPFQILDQTGVGQLVKMGVEKGRTT
                     KPDLKIGICGEHGGEPSSIEFCHRVGMDYVSCSPYRVPIARVAAAQAAIKN"
     gene            2365..>2526
                     /gene="rpsF"
                     /locus_tag="P5236_05220"
     CDS             2365..>2526
                     /gene="rpsF"
                     /locus_tag="P5236_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRU63797.1"
                     /translation="MLNHYETVFILTPVLSETQMKETVEKFKSLLLENGAVITNEENW
                     GLRKLAYPIQ"
BASE COUNT          870 a          382 c          523 g          751 t
ORIGIN      
        1 attttatatc ggaaaatgga aggtatccct gacgattggg gtactgctgt tactgttcag
       61 gcaatggttt ttggtaatat gggtgatact tctgctacag gtgtagcttt tacacgaaat
      121 gctgctacag gcgaaaatgt ttttaatgga gaatatttag ttaatgcaca aggtgaagat
      181 gtagtagctg gtattcgtac tcctcttcaa attacaaaag caggttcaca acaatgggca
      241 aaacttgctg gaatttcaga agaagaaaga agaacaaaat atccttctat ggaagaagct
      301 ttaccggata tttataaaga actaaatgaa attcaacaaa agcttgaaaa tcattataaa
      361 gatatgcaag atattgagtt tactgttcaa gaaggtaaac tttggctttt gcaaacaaga
      421 agcggtaaaa ggacaggaac ggctatggtt aaaattgcca ttgacatgct tcacgagggc
      481 attatagatg aaaaaacagc tttgttaaga attgatcctt taaaattaga tgaattactg
      541 catccaattt tcgataaaga atcgttgaaa aatgtcaaag ttatagctaa aggtttgcca
      601 gcttcaccgg gagctgcgag tggaaaaatt gttttcaatg ctgacgatgc tgctgaatgg
      661 gcaaaacaag gcgaaaaagt tatcatggta cgcattgaaa cttcacctga agatttggca
      721 ggtatggcag cagctcaggg aatattaact gcacgcgggg gaatgacttc tcatgctgca
      781 gtcgtagctc gtggaatggg gaagtgctgt gtatcaggag ctggagcgtt gaaaattgat
      841 tatgtcaaca gaactattca aatcaatgat ttagttttaa aagaaggtga ctatatttct
      901 atcaatggat ccacaggaga agtttatctc ggaaaagttc ctaccaaaga acctgagtta
      961 gatccctact tcattgaatt attgacatta gctgacaaat acgctgatct aagagttaga
     1021 accaatgcag acactccgcg tgatgcttct gtggcaagaa aatttggtgc tacgggtatt
     1081 ggtttatgta gaactgaaca catgttcttt gaaggaaata agattaaagc catgcgagaa
     1141 atgattttgg cggaagatac tgaaggtaga aaaaaagctt tagcaaaact tcttccttat
     1201 caaagagctg attttgaagg aatttttgaa gttatggagg gactgcctgt tactatccgt
     1261 cttttagatc ctcccctaca tgaatttgtt ccccacgatg aaaaaggtca acaggaaatg
     1321 gctgaagcaa tgggtgttcc tttaaagaaa atacaagaac gcgtagaatc attgcgtgaa
     1381 caaaatccaa tgttaggtca tcgtggatgc aggttaggaa atactttccc agaaattact
     1441 gaaatgcaaa cacgtgctat tctcgaagct gtcttgaatc tgaaagcaaa aggaatcaaa
     1501 attattccag agatcatggt cccattagta agtgtaaaaa aagagttagt tgagcaggaa
     1561 agaattattc gtgaaacagc aaataagata tttgaagaga aaggagatac tattgaatat
     1621 aaagtaggaa ctatgataga agttccccga gcagcattaa ctgctaatga aatagctgaa
     1681 aatgctgact ttttctcatt tggtaccaat gaccttactc agatgacatt tgggttttca
     1741 cgagatgata ttggttcttt tttaccaatt tatttagaga aaaaaattat tgagtcggat
     1801 cctttccaga tacttgatca aacgggcgta gggcaattag taaaaatggg tgttgaaaaa
     1861 ggtcggacta caaaaccaga tttgaaaatt ggtatttgcg gtgaacatgg tggcgaacct
     1921 tcttctatag aattttgtca tcgtgtggga atggattatg taagttgttc tccttataga
     1981 gttcctattg ctcgggtggc tgcagctcaa gctgccataa aaaactaaga atagatattt
     2041 acaaaaaggg tagttcaatt gagctgccct ttttggtaaa ccgagaatta tcattgataa
     2101 aaaaaatttc agccaaacac atcgttactc ggataaatac ttaaaaaaaa gaaaatatca
     2161 tctttttgtc atatactaaa aaaacatttg tgatgatttt caatatttta tatacttttc
     2221 atttaagtca taaaacttgt ttctaatgac agttctataa tagaattaaa aaagctgttg
     2281 aatccttttg tcaatcaaat aattatttat aactttgcaa acgcttttaa gaaaaatgtc
     2341 aaatttaaaa cgttaataat tagtatgtta aatcattatg aaaccgtttt cattttaact
     2401 cccgttttgt ctgagactca gatgaaggaa acggtagaaa aattcaagtc ccttttgctt
     2461 gaaaatggtg ctgtaattac caatgaggaa aattggggat tgcgaaaatt agcttatccc
     2521 atccag
//
LOCUS       DAOTKS010000239         2524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_33475_length_2524_cov_3.689753, whole genome shotgun sequence.
ACCESSION   DAOTKS010000239 DAOTKS010000000
VERSION     DAOTKS010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33475_length_2524_cov_3.689753"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2278)
                     /locus_tag="P5236_05225"
     CDS             complement(<1..2278)
                     /locus_tag="P5236_05225"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HRU63798.1"
                     /translation="MSKKQTIIVLFFLLLTVVAFGAIPPGYYYFARNKKKAELKTALA
                     QIAAPLKVLKYGSGPGYTWEGFYYTDQNPDGSVIDMYSPKVRYFNGFSSVNGMHIEHS
                     LPKSWWGSIENNAYKDLFHLYPADGETNSIKNNFPLGEITIPIFDNGVSKIGKNSFGD
                     FYTDNCFEPADEYKGDFARSYLYVSTIYENFAPLWNSPMMDNNTYPVWKPWAMDLLLK
                     WHREDPVSEKEEKRCEAVYNIQGNRNPFIDYPELVEYIWGKDTTNIFPFPEETEAFLL
                     SPRKGSEIDFGVLLQNDTKTLEILIQGINITSEVKISLIKKNSSFHLSSSTISKDEIL
                     NGTNILLTFQPQTSGWFNDTLSISEGGLKENLYIPLKALASPDFITLEPDDVTPVGGI
                     LRWIYDPQAIDYLLDVYQGDAQAGDLIISGYAEGSSWNKAIELFNGTGKPIDLSKYSL
                     QKQTNAYGPFGSNFKLNGILQNGKTYLLVRKDANDVLRNKANILTDSLLAFDGNDPIA
                     LVRNGIIIDMIGDPNLGGNVYWGQDVTLKRKSYVTHPISTCNFAEWDRYEKDDFTKLG
                     SHHMDFQTTKSYVLQNYSTGGDAEFIIDQLMPESTYTYSVKAIRSNEEIVSANTMQIH
                     TTRLDMPIAMDALNVHSTGFIANWEDHLYTQNFLLDVFQLQETADTTEIETFDNVGSN
                     GKPLPTGWTGTASGNYTSSISSGETPPSIALKNDGEYLQTKTYPLPVKNLSFMYRFPS
                     EATGSSLILEGIKENGNIS"
BASE COUNT          857 a          451 c          409 g          807 t
ORIGIN      
        1 gacttatatt accattttcc tttattcctt ccaaaataag agaagaaccc gttgcttcag
       61 aaggaaaacg atacataaag gaaagatttt ttacaggtag aggataggtt tttgtttgca
      121 ggtattctcc atcatttttc aaggcaatag aaggaggcgt ttctcccgaa ctgatagaag
      181 acgtataatt tccacttgct gtgcctgtcc aacctgtggg taaaggtttt ccattacttc
      241 ccacgttatc aaaagtttca atttcagtag tatcagccgt ctcttgtaac tgaaaaacat
      301 cgagtaaaaa attttgggta taaagatgat cttcccaatt ggcaataaaa ccagttgagt
      361 gcacatttaa agcatccata gcaattggca tgtctaaacg agtagtatga atctgcatag
      421 tgttagccga aacaatttct tcatttgaac gaattgcctt gaccgaataa gtataagtgc
      481 tttcaggcat cagttgatca ataataaatt ccgcatcacc acctgtggaa taattttgta
      541 aaacataact tttagttgtt tgaaaatcca tatgatgaga gcccagcttt gtaaaatcat
      601 ctttttcata tctatcccat tcagcaaaat tgcatgtcga aattggatga gtaacatacg
      661 attttcgttt cagcgtcaca tcctgtcccc agtaaacatt ccctcccaaa ttgggatctc
      721 ctatcatatc aataataatt ccattcctta ctaaggcgat gggatcattg ccatcaaaag
      781 ctaataaaga atccgttaaa atattggcct tattacgaag tacatcatta gcatctttgc
      841 gaacgagcaa ataagtttta ccattttgca atataccatt taatttgaaa ttagaaccaa
      901 agggaccata agcattggtt tgtttttgta aagaatattt tgataaatca attggtttac
      961 ccgtaccatt aaataattct attgctttat tccaactgct cccttctgca tatccagaaa
     1021 taatcaaatc acctgcttga gcatcacctt gataaacatc gagaagatag tcaattgctt
     1081 gaggatcgta aatccaacgc aaaatgcctc ccaccggagt aacatcatcg ggttctaaag
     1141 taataaaatc aggcgaagcc agagctttta gaggaatata aagattttct ttaagtcctc
     1201 cttcagaaat ggaaagggta tcgttgaacc accctgacgt ttgtggctga aaagtgagca
     1261 aaatattcgt accatttaaa atctcatctt ttgaaatagt ggaagaagaa agatgaaacg
     1321 aagagttttt ttttataagt gaaattttta cctcggaagt aatattgatt ccttgaataa
     1381 gtatttccaa tgttttggta tcgttttgca gcagcactcc gaaatctatt tcagaacctt
     1441 ttcgtggaga aagtaaaaaa gcctccgttt cttcgggaaa tgggaaaata tttgtcgtgt
     1501 ctttacccca aatgtattct accagttcag gataatcaat aaaaggattt cgattccctt
     1561 gaatattgta aaccgcttca catcgttttt cttccttctc acttacagga tcttctctgt
     1621 gccattttaa aagtaaatcc atagcccacg gtttccaaac aggataggta ttattatcca
     1681 tcataggaga attccataat ggggcaaaat tttcataaat agtagaaaca tataaatatg
     1741 atcgagcaaa atcgccttta tattcatcgg caggttcaaa acaattatcg gtataaaaat
     1801 ctccaaagct atttttccct attttgctca caccattatc aaaaatgggg atagttattt
     1861 cacctaatgg gaaattattt ttaatggaat tggtttcgcc gtctgccgga taaagatgaa
     1921 acaaatcttt ataagcatta ttttcaatag aaccccacca gcttttggga agagaatgct
     1981 caatatgcat tccgtttaca ctggaaaagc cgttaaaata tcgaacttta ggtgaataca
     2041 tatcaattac tgaaccatct ggattttgat cagtataata aaatccttcc catgtgtaac
     2101 cgggaccact gccatatttt aaaactttta aaggagcagc aatttgtgcc agagcagttt
     2161 tgagttcagc ttttttcttg ttgcgagcaa aataatagta tcctggtgga attgcaccaa
     2221 aagcaactac agttaacaat aagaaaaaaa gtacgatgat agtttgcttt ttactcatta
     2281 atggtaaagt ttcattatat tattgtcttt tttcattgta aaatgaagta tagcaaaaca
     2341 aaacaaggag aaaaagaata tttcaataaa aaatttgatt aattttgaaa aaaatttaaa
     2401 cgcaaaaatt atgaaataat aagtttatta tccagataca ataacaaata tacacatttg
     2461 tgagaaaatg aaagaaagtt ctttgacaaa ttttgaataa atctaaatct ttccttcctc
     2521 cctt
//
LOCUS       DAOTKS010000240         2514 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_33660_length_2514_cov_3.474176, whole genome shotgun sequence.
ACCESSION   DAOTKS010000240 DAOTKS010000000
VERSION     DAOTKS010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33660_length_2514_cov_3.474176"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            274..930
                     /locus_tag="P5236_05230"
     CDS             274..930
                     /locus_tag="P5236_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63799.1"
                     /translation="MKTYIKKFFYFPVFLSFLLAACINNEPKEPYIYEKNPQYTWGYA
                     VFYGDYYAEYGNENNVLSISLFTNGLGINENNYLIGTGQYLYLEDIFVTPTDTLLPDG
                     IYTIDKSGESFTISPGEHLSIDGVIYTVGATITYLDIYLLNPVEKLITEGTMNVSRQD
                     TIYTISCNFKTDDNLELKGNFTGILPHFDQSLKKNSESLRMLRHTKRSFILSRLNSSK
                     "
     gene            1093..2034
                     /locus_tag="P5236_05235"
     CDS             1093..2034
                     /locus_tag="P5236_05235"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HRU63800.1"
                     /translation="MNYQSIPPKQPGLSQFFNIDLREIVPFIHWSFFFREWRIPGKFE
                     EIEKVSSCVSCKQSWLQNFSGSARDKAEEALKLFQDAQNMLQQFSVEKSVQINAAVGL
                     FSAFAQEEDIHILYENKEFIIPTLRQQHPSTDGFCYALSDFLNVENDYIGTFATVVQG
                     TEKLIQHFENKKDIYSSMMVKTLADRLAEATAEWLHYQVRTKIWNYASDELLEIENIL
                     KNKYQGIRPAIGYPSLPDQSIIFDLEPLLQLNTIGIKVTENGAMYPPPSVAGLYFAHP
                     QSKYFNVGKIDDQQLKDYARRRGKTIEEMKKWLAANI"
     gene            2088..2306
                     /locus_tag="P5236_05240"
     CDS             2088..2306
                     /locus_tag="P5236_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HRU63801.1"
                     /translation="MLNIKSELLKIKGMSCSHCAARVEKALNTIDGVSAKVDLNSNTA
                     KVSLLKEVSDEVLKKAVEDVGYEVTGIL"
BASE COUNT          874 a          341 c          417 g          882 t
ORIGIN      
        1 acaaacctta ttaccttatt taaaacaaat taaaaacaca aatcgaaaga aagtaacaat
       61 tttatctaaa taaagtaata aggttggaag aaagggaaat tttagtgtta taaaggttat
      121 atgttttaat aaggtttaaa agacaaaata aggaaactga cttcaaacaa atgaacacaa
      181 acatatattt taagtatgag aatgaaattt tttaatttct tacatgttcc cacataattg
      241 atattgtaaa aacttcataa aatttatttt cggatgaaaa catatattaa aaagtttttc
      301 tattttcctg tttttctatc atttttactg gctgcttgta ttaataatga accaaaagaa
      361 ccttatatat acgagaaaaa tcctcaatat acatggggct atgcggtatt ttatggtgat
      421 tattatgctg aatatggaaa tgagaataac gtgttatcta tttcgctgtt taccaatgga
      481 ttgggtatta atgaaaacaa ttatttaatt ggaacgggtc aatacttgta tctggaagat
      541 atttttgtaa caccgaccga tactttgtta cctgatggta tttatactat tgataaaagt
      601 ggagaatctt ttactatttc tcctggtgaa catttgtcaa tagacggcgt catttataca
      661 gttggagcaa ccattaccta tttagacatt tatttgctga atcctgtcga aaaattaatt
      721 actgaaggaa caatgaatgt tagtcgtcaa gatactatct atactattag ttgcaatttt
      781 aaaacagacg acaatcttga actaaaagga aacttcactg gtatattgcc acattttgat
      841 caatcgttga agaaaaattc agaaagttta cgaatgttaa ggcatacgaa acgttctttt
      901 atcttatcca gattaaattc ttcgaaataa aaaggctttt ctttctgatt tttaactttt
      961 atttttgttt acttgtagat aaaaagactg tttaaatttt ttataaatgt ctaaaatata
     1021 attttcagtg acatttcttc aacaaattgg gtatcccttt tcgttaattc aaaatttatt
     1081 ttgagaaaag agatgaatta tcaatccata ccaccaaagc aaccaggatt aagtcagttt
     1141 tttaatattg atttacgaga aattgtacct ttcattcatt ggagtttttt ctttcgcgag
     1201 tggcgaattc ctggaaaatt tgaagaaatt gaaaaagttt cttcttgtgt ttcttgcaag
     1261 caatcgtggt tacagaattt ttccggttca gctcgtgata aagcagagga agccttaaaa
     1321 ctttttcagg atgcccaaaa tatgcttcaa cagttttctg tagaaaaatc tgttcagatt
     1381 aatgctgcag taggcttatt ttctgccttt gctcaagaag aagatattca tattttatat
     1441 gaaaataaag aattcatcat tcctacactt cgtcagcagc atccttccac agatggtttt
     1501 tgctatgctt tatctgattt tttgaatgtt gaaaatgatt atattggtac ctttgctaca
     1561 gtagtgcaag gaacagaaaa attgattcag cattttgaaa ataaaaaaga tatttacagt
     1621 tccatgatgg tgaaaacttt agcagatcgt ttggcagaag ctactgctga atggctacat
     1681 tatcaggttc gaacaaaaat ttggaattat gcgtctgatg aactactgga aatagaaaat
     1741 attttgaaaa acaaatatca aggtattcgt cccgctattg gttatccttc tttaccagat
     1801 caatctatta tttttgactt ggaacctttg ttgcaattga atacaattgg cataaaagta
     1861 acggaaaatg gagctatgta tccgccaccg tcagtagcag gattgtactt tgctcatcca
     1921 caatcaaaat attttaatgt aggcaaaatt gatgatcaac aattgaaaga ttatgcacgt
     1981 cgacgtggaa aaacaataga agaaatgaaa aaatggttag ctgcaaacat ctaattgttc
     2041 tatatcttga aaaacaaaat caatttaatg atttttgaac ttgtaaaatg ttgaatatca
     2101 aaagtgaatt attaaaaatc aaaggaatga gttgctctca ttgtgcagct cgggttgaaa
     2161 aagcgttgaa tacaattgat ggcgtttctg caaaagtaga ccttaattca aacactgcaa
     2221 aagttagctt attaaaagaa gtcagcgatg aagttttaaa aaaagcagtt gaagatgtag
     2281 gttatgaagt aacaggtatt ttataaaaaa ttttcttttc gatagttatt tataaagaaa
     2341 tgaatttaga attgagaatt aaatcagtat ttttattttg catctgattt gttgacttct
     2401 tatgttttaa gtttactttc tttttgattt gtgtcaaaac aattgagtgg agcaattatt
     2461 ggcgtgttct atcaagcgtt tatctgctgt tcgctgggga gtaactcgag attt
//
LOCUS       DAOTKS010000241         2503 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_33833_length_2503_cov_4.510621, whole genome shotgun sequence.
ACCESSION   DAOTKS010000241 DAOTKS010000000
VERSION     DAOTKS010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33833_length_2503_cov_4.510621"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="P5236_05245"
     CDS             <1..183
                     /locus_tag="P5236_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HRU63802.1"
                     /translation="NGWMIRFSASPIFDSHMQHIEEGIEPDIQKDMSKRGAINGYDSI
                     IEEAIRQINSYFSKTY"
     gene            243..779
                     /locus_tag="P5236_05250"
     CDS             243..779
                     /locus_tag="P5236_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRU63803.1"
                     /translation="MRKLKVTEMNRLTVEEFKQQSKLPLVIVLDNVRSLHNVGSVFRT
                     ADAFRVEEIYLCGITRIPPHPELHKTALGAEDAVNWRYFQDTHEAIDDLKTKGYSIFS
                     IEQAEGSTLLQNLQLPKNGKFAVVLGNEVKGVQQTVIDLCDGCIEIPQFGTKHSLNVS
                     VTCGIIVWEFFKKIALSL"
     gene            794..1129
                     /locus_tag="P5236_05255"
     CDS             794..1129
                     /locus_tag="P5236_05255"
                     /inference="COORDINATES: protein motif:HMM:NF013422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HRU63804.1"
                     /translation="MDWKEKLKEIKATLPQVEDQEKEDEKATAKNIQKEPLRIQLEKR
                     NNKVVTLVSEFQGSEEELKDLARLLKMKCSSGGTYSDNQILIQGDFRVKIAEILENLG
                     YKVKKINFK"
     gene            1388..2047
                     /locus_tag="P5236_05260"
     CDS             1388..2047
                     /locus_tag="P5236_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63805.1"
                     /translation="MYNESLFLSAISESYKNFVEYGPRSNKKLIPLHTFVGETLQNIW
                     GKDFELYFISESTKEFTVEGKYYPKAIDITVAQNGIPVFCFGVKFITSNYKQNSNNYF
                     ENMMGETANIQAKKDLPYAQLIILRHETPYYEKTVQKSGRKKATKIEIINEYYLQKYL
                     NLIFDSPQAHRPFAMGIILINVNEQTCNVEKVNLNSVFEADFAELFEKKLSITAEFKL
                     H"
     gene            complement(2093..>2503)
                     /locus_tag="P5236_05265"
     CDS             complement(2093..>2503)
                     /locus_tag="P5236_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318802.1"
                     /note="uses the energy from reduction of ubiquinone-1 to
                     ubiquinol to move Na(+) ions from the cytoplasm to the
                     periplasm; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HRU63806.1"
                     /translation="REMLYIGGGAGMAPLRSHILHLLKTLQVRDRKISYWYGARSKNE
                     IFYEEDFRELEKEFPNFTFQIALSEPLPEDNWTGYVGFIHSVIYDNYLKNHPEPEEIE
                     YYMCGPGPMAKAVEKMLDDLGVPREMLMFDDFGA"
BASE COUNT          893 a          370 c          428 g          812 t
ORIGIN      
        1 aatggctgga tgattcgttt ttctgcttct cctatttttg acagccacat gcaacatatt
       61 gaagaaggga ttgaacctga cattcaaaaa gatatgagta aacgaggggc aataaatggc
      121 tatgattcga tcattgaaga agcaatccga caaataaatt cttacttttc aaaaacttat
      181 tagcaaaata acaaataata ttttgattta tttttaggaa atgtttttga tgtaaaaaaa
      241 ttatgcgaaa gctgaaagta accgaaatga atcgcctcac tgtggaagaa ttcaagcaac
      301 aatctaaact tccgctggtg attgtgctgg acaatgttag gagtttacat aatgttggtt
      361 ctgtatttcg caccgctgat gcttttcgtg tggaagaaat ttatttgtgt ggcattacac
      421 gtatacctcc gcatcctgaa ctccataaaa cagctttagg agcagaagat gcagtaaact
      481 ggcggtattt tcaggatacg catgaggcaa ttgacgattt aaagacgaaa ggttacagca
      541 ttttttctat tgaacaagct gaaggaagca ccttattgca aaatttacaa ttgcccaaaa
      601 atggcaaatt tgctgtagtg ctgggtaatg aagtaaaagg tgtacagcaa acagtgatcg
      661 acctgtgcga tggatgtatt gaaatccctc aatttggtac caaacattca ttgaatgtaa
      721 gcgttacgtg cggcatcatt gtttgggaat ttttcaaaaa aatagcacta tctttataaa
      781 aatactttca aatatggatt ggaaagaaaa actgaaagaa ataaaagcaa ctttgccaca
      841 agtagaagat caagaaaaag aagatgaaaa ggcgacagca aaaaatattc aaaaagagcc
      901 gttgcgaatt cagttagaaa aacgaaataa caaagtggtg actttagtca gtgaatttca
      961 aggcagtgaa gaagagttga aagatctggc tcgtctattg aaaatgaaat gtagtagtgg
     1021 tggcacttac tctgataatc aaattttaat tcagggagat tttagagtaa aaattgcgga
     1081 aattttggaa aatctgggat ataaagtgaa aaaaatcaat tttaaataat agtttttaca
     1141 aatttattcc ttaaacgaga atttttattt tataaagttt tttcttcact ttttagttag
     1201 aataaagatt taaaatattc ttatcaatca aaaaattatc aaagaacttt ctttaattta
     1261 accacaaatt tgtatatttg tttttattgt gcgttgtttt ttctcatttt ttattttcgt
     1321 tacgtttctt tttatattga gaaaaatatc aatgaagttg tagaacctta caattaaatt
     1381 caaatttatg tataatgaat ctttattttt aagcgcaatt tcggaatcct ataagaattt
     1441 tgttgaatat ggacccagaa gcaataaaaa attaattcct cttcacacat ttgttggaga
     1501 aactttacaa aacatttggg gaaaagattt tgaactctat tttataagcg agagcacaaa
     1561 agaatttact gttgaaggaa aatattatcc taaggcaatt gacataacag tagcacaaaa
     1621 tggaatacca gttttttgtt ttggagttaa atttattacg agtaactaca aacaaaattc
     1681 taataattat tttgaaaata tgatgggtga aaccgctaat attcaagcaa agaaagattt
     1741 accgtatgct caattaatca tattaagaca tgaaacgccc tattatgaaa aaacggtaca
     1801 aaaaagtggt agaaaaaagg caacaaaaat tgaaattatc aatgaatatt atttgcaaaa
     1861 atatttgaat ttaatttttg attctcctca ggctcataga ccttttgcaa tgggcatcat
     1921 tttaattaac gttaatgaac aaacttgtaa tgttgaaaaa gtaaatctaa actctgtttt
     1981 cgaagcagat tttgctgaat tatttgaaaa aaaattatct ataactgctg aattcaagtt
     2041 gcattaatta cttgaattta cgttataacg ccctgtggaa aaattatttg ctttatgccc
     2101 caaaatcatc gaacatgagc atttctctgg gtacccctaa atcatctaac attttttcaa
     2161 ctgcttttgc cataggtccc ggtccacaca tgtaatattc aatttcttct ggttcagggt
     2221 gatttttcag ataattatca tatattacgc tatgaataaa gcctacataa cctgtccaat
     2281 tatcttctgg caaaggttcg ctgagtgcaa tttggaaagt aaagtttggg aattcttttt
     2341 ctaattcacg gaaatcttct tcataaaaaa tttcattttt cgaacgagcc ccataccagt
     2401 aggatatttt cctgtcgcgt acctgcaatg ttttgagtaa atgcaaaata tgagagcgca
     2461 gaggagccat tcctgcacct ccaccgatat aaagcatttc gcg
//
LOCUS       DAOTKS010000242         2503 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_33835_length_2503_cov_4.015523, whole genome shotgun sequence.
ACCESSION   DAOTKS010000242 DAOTKS010000000
VERSION     DAOTKS010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33835_length_2503_cov_4.015523"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..110)
                     /locus_tag="P5236_05270"
     CDS             complement(<1..110)
                     /locus_tag="P5236_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRU63807.1"
                     /translation="MINKIERRQRIKLRIRNKVSGTNKKPRMSVFRSNAQ"
     gene            complement(134..688)
                     /gene="rplF"
                     /locus_tag="P5236_05275"
     CDS             complement(134..688)
                     /gene="rplF"
                     /locus_tag="P5236_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRU63808.1"
                     /translation="MSRIGKLPIKIPTGVSVSVDNNNLVTVKGPKGTLTQKVNPIITV
                     TVKDGMCSVTRPNDEKVSRQLHGLYRALINNMVIGVSEGFKKTMELVGVGYRVSSQGQ
                     TLEFSLGYSHNIFLQLPDEIKVETKSERNQNPLIILESCDKQLLGQVCAKIRSFRKPE
                     PYKGKGIKFAGEVLRRKAGKSAGK"
     gene            complement(709..1104)
                     /gene="rpsH"
                     /locus_tag="P5236_05280"
     CDS             complement(709..1104)
                     /gene="rpsH"
                     /locus_tag="P5236_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRU63809.1"
                     /translation="MTDPIADYLTRLRNAIQANHRVVEIPASNLKKEITRILFENGYI
                     LNYKFLDDGPQGTIKVALKYHPVYKVNAIKKLIRVSTPGLRKYVGYKDMPRVLNGLGI
                     AILSTSKGVMTNKEAIKQKVGGEVLCYVY"
     gene            complement(1240..1509)
                     /gene="rpsN"
                     /locus_tag="P5236_05285"
     CDS             complement(1240..1509)
                     /gene="rpsN"
                     /locus_tag="P5236_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HRU63810.1"
                     /translation="MAKESLKAREVKRAKLVAKYAAKRQLLLAEGNYEALQLIPKNAS
                     PVRLHNRCKITGRPKGYMRQFGLSRIQFRELASKGLIPGVKKASW"
     gene            complement(1525..2070)
                     /gene="rplE"
                     /locus_tag="P5236_05290"
     CDS             complement(1525..2070)
                     /gene="rplE"
                     /locus_tag="P5236_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRU63811.1"
                     /translation="MNTANLKQDYKERIVPNLMKEFGYTSVMQVPRLEKIVLNQGLGI
                     AVSDKKIIDTAINELTAITGQKAVATLSKKDISNFKVRKKMPIGVRVTLRGERMYEFL
                     ERLIRVALPRIRDFKGISNKLDGHGNYTLGITEQIIFPEVNIDAINRIMGMNITFVTT
                     ARSDEEGFALLKEFGLPFKKN"
     gene            complement(2073..2384)
                     /gene="rplX"
                     /locus_tag="P5236_05295"
     CDS             complement(2073..2384)
                     /gene="rplX"
                     /locus_tag="P5236_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRU63812.1"
                     /translation="MSKLHIKKGDTVYVNTGVDKGRTGRVLKVLVKEQRAIVEGINLV
                     SKHMKPNARYPQGGIVKQEAPIHISNLNLVENGKPVRIGRKLNKDGELVRISKKTGEE
                     I"
     gene            complement(2407..>2503)
                     /locus_tag="P5236_05300"
     CDS             complement(2407..>2503)
                     /locus_tag="P5236_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uL14 family ribosomal protein"
                     /protein_id="HRU63813.1"
                     /translation="GELRGSRIFGPVARELRATNMKIISLAPEVL"
BASE COUNT          721 a          491 c          328 g          963 t
ORIGIN      
        1 atctgagcat tgcttctaaa aacactcatt cttggttttt tatttgttcc tgaaacctta
       61 ttacgaatac gtaattttat gcgttgtctt ctttctattt tgttaatcat aatttctaaa
      121 tttacgtatt agattattta cccgctgatt ttccagcttt tcgacgtaaa acttcacctg
      181 cgaatttaat tccttttccc ttataaggtt ctggttttct gaacgaacga attttagcac
      241 aaacctgacc taataattgc ttgtcgcaag attctaaaat aataagagga ttttggttac
      301 gttcactctt tgtttctact tttatttcgt caggaagctg caaaaaaatg ttatgcgaat
      361 atcccaaaga gaattcaagt gtttgccctt gtgaagaaac tctgtaacct acccctacca
      421 attccatagt ttttttaaat ccttctgaaa ctccaattac catattgtta atcagagctc
      481 tatacaatcc atgcaattgt cgacttactt tttcatcgtt tggacgagtt acactgcaca
      541 ttccatcttt aacagtcact gtaataattg gattaacttt ttgtgtaagt gttccttttg
      601 gtcctttaac tgttactaaa ttattgttat ctacacttac agaaactcct gtaggaattt
      661 ttataggtaa tttaccaatt ctcgacataa ttttctctcc tctttacttt aataaacata
      721 acataaaact tctcccccta ctttttgctt aatcgcttcc ttattagtca tcactccttt
      781 tgaagtagaa agaatagcta ttcctaatcc attcagcaca cgtggcatat ctttatagcc
      841 aacatactta cgaagtccag gtgtagaaac acgaattaat tttttgatag catttacttt
      901 ataaaccgga tgatatttta aagctacttt gatagtacct tgaggtccat cgtctaagaa
      961 cttataattc aaaatataac cgttttcaaa taaaatacga gttatttctt tttttaaatt
     1021 tgatgcagga atttcaacca cacgatgatt tgcttgtatt gcattccgca aacgagttaa
     1081 ataatctgca ataggatctg tcatatattt attttttaaa ttggtcccga ctgccgggat
     1141 aatatttaat ttttctgttt cttagaattt tattttcctc ttctcgctaa acttttttaa
     1201 agccaactct ttattaggaa aattatcaat tttttttaat taccagcttg ctttttttac
     1261 accagggatt aatcctttcg aagccagttc acgaaattga atacgggata agccaaactg
     1321 acgcatatac cctttaggac gtcctgttat tttacaacga ttatgtaaac gaacaggaga
     1381 agcattcttc ggaattaatt gaagggcttc gtaatttcct tcagcaagta acaactggcg
     1441 tttagctgca tatttggcaa ctaattttgc tcttttcact tcccgtgctt ttaatgattc
     1501 tttggccatg tttctttctt tttattaatt tttcttaaac ggtaatccaa attcttttaa
     1561 taaagcaaaa ccttcttcat cactgcgtgc tgttgtaaca aaagtaatgt tcatccccat
     1621 aatacgattg atagcatcta tattcacttc aggaaaaata atttgttctg ttatacctaa
     1681 cgtataattt ccatgtccat ctaatttgtt gcttattccc ttaaaatcac gaatacgcgg
     1741 taatgcaaca cgaattaaac gttccaaaaa ttcatacatt ctctcgcctc taagagtaac
     1801 tctgacgcca attggcatct tttttcttac cttgaaattt gaaatatctt tctttgataa
     1861 ggtagcaact gctttttgac ccgtgatagc tgtcaattca tttatagcag tatcaataat
     1921 ttttttatct gatacggcaa ttcctaaacc ttgatttaat acaatttttt ctaatcgtgg
     1981 aacttgcata acagaagtat acccaaattc tttcattaaa ttaggaacta tccgttcttt
     2041 ataatcttgt tttaagtttg ctgtattcat tcttaaattt cctctccagt ttttttagaa
     2101 attcttacta attctccatc cttatttaat tttcttccaa tgcgaaccgg tttaccattt
     2161 tccactaaat tcaaattgga aatatgaatg ggagcttcct gctttactat tcctccttga
     2221 ggatatctgg cattaggttt catgtgtttg gaaactaagt taatgccttc aacaatagct
     2281 cgttgttctt ttactaacac ttttaaaact cttccagttc tgcctttatc aacaccagta
     2341 ttgacataaa ctgtatcacc tttctttata tgtaatttgc tcatcacttt atattttttc
     2401 aaagaattaa agcacttctg gtgcaagaga tatgattttc atattagtag cacgtaattc
     2461 tcgagccaca ggtccaaaaa tacggcttcc tctaagttct cct
//
LOCUS       DAOTKS010000243         2489 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_34094_length_2489_cov_2.004108, whole genome shotgun sequence.
ACCESSION   DAOTKS010000243 DAOTKS010000000
VERSION     DAOTKS010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34094_length_2489_cov_2.004108"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="P5236_05305"
     CDS             complement(<1..496)
                     /locus_tag="P5236_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4968 domain-containing protein"
                     /protein_id="HRU63814.1"
                     /translation="MKNFISYFFIIVSLLFPFSLFSQNNPKANSSAIVISGNMRFTVL
                     TPEIIRIEWSEKNIFEDRASFVVVNRNLPVPTFTTREENGYLFINTSKLELQYKIGTY
                     PINSDVCNPNLQISLQVNGENIVWYPGKKDPYNLKGTTRTLDNAEGDVRSWLEDGIIS
                     RSGWA"
     gene            complement(597..1694)
                     /locus_tag="P5236_05310"
     CDS             complement(597..1694)
                     /locus_tag="P5236_05310"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HRU63815.1"
                     /translation="MKNKLLIFTITVFCIILLATFFACNNSKEEVLIWTETSVPDTLY
                     QNPLFEPDLADPSFIKASDGWFYAYGTENEWESGKNRLIPIIKSKNMIKWEYVADAFQ
                     TKPTWHSGGYLWAPQILFNSNDGYYYLYYSFSSWGDSNPGIGVAKSQNPTGPFEDLGK
                     VLDVQSSGVRNSIDQFYIEVGSGRNKKAYLFWGSFAGIYGVEMSDMKTPKLDTKFKIA
                     GNSFEGSYIYEHDGYYYYFGSSGNCCEGANSLYHLTVARSTNIKGPYVSKSGASILND
                     GVEGTPFLYGDKNVGWVGPGHNGEIIKDDKGRYFIIYHAIKYSNPLLPNGATRRPLMM
                     DEIIWDADGWPTIAGAVPSNTRKDAPYFESK"
     gene            complement(1753..>2223)
                     /locus_tag="P5236_05315"
     CDS             complement(1753..>2223)
                     /locus_tag="P5236_05315"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRU63816.1"
                     /translation="VYWKKYTNYDTRTTEDHENNGINIRVVRLADIYLMYAEALNELG
                     RTSESYQYIDKVRRRVNLPDLENSTVFTGIGNDPAKMREQIIHERSCELAGESWRWLD
                     LERWGFFDSADKIAWLTNRDSEFANFVIGKSNRFPIPYREVNLVKGLKQNPGYE"
     assembly_gap    2224..2233
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2234..>2489)
                     /locus_tag="P5236_05320"
     CDS             complement(<2234..>2489)
                     /locus_tag="P5236_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63817.1"
                     /translation="GVTQLSHERAKFLGLPANGCSYDDGTASNIVKTDLEQERTIDGK
                     VDPRLKNTLFYYDPNNLSELFYGKTWLQWENDPNSSVKRAK"
BASE COUNT          795 a          480 c          370 g          834 t
ORIGIN      
        1 ccgcccaacc agaacgagat atgataccat cttcaagcca actgcgaaca tctccttctg
       61 cattgtcgag agttctggtt gttcctttta aattgtatgg atcttttttc ccgggatacc
      121 aaacaatatt ttcaccgttg acttgtaaag atatttgtag atttggatta caaacatcac
      181 tatttattgg ataagtacca attttatatt gtaactcaag ttttgatgta ttaataaata
      241 aatatccgtt ttcttcacga gttgtaaaag ttggaacagg taaattacga tttacaacga
      301 caaatgaagc tctatcttca aaaatatttt tttcgctcca ttcaatacga attatttcag
      361 gagttagaac ggtaaatctc atatttcctg aaataactat agcagaacta ttcgctttag
      421 gattattttg agaaaatagc gaaaagggga acaataaact aacaataata aaaaaataag
      481 atataaagtt tttcatgtat ttaaaacttg attttaatgc taattacaaa aaaattatta
      541 attttccact aaaaactaag tattctttgt aattagcatt tcataattca acaatattat
      601 tttgattcaa aataaggtgc atcttttctt gtgttacttg gcactgcccc tgctattgta
      661 ggccatccgt ctgcatccca tataatttca tccatcatta acgggcgtcg tgttgctcca
      721 ttagggagca aaggatttga atattttatg gcatgataga taataaaata tcgtccttta
      781 tcatccttaa taatttctcc attatgaccg ggaccaaccc aacccacatt tttatctcca
      841 tataaaaatg gagttccttc tacgccatca ttgagaatac tcgctccgct tttagaaaca
      901 taagggcctt taatattagt ggatctggca acagttagat gatacagact gtttgctcct
      961 tcgcagcaat ttccactcga tccaaaataa taataatatc catcgtgttc ataaatatac
     1021 gaaccttcaa aagaattgcc tgcaatttta aatttcgtat caagttttgg agttttcata
     1081 tcagacattt ctacaccata aattccagca aaactgcccc aaaaaagata tgcttttttg
     1141 tttcgaccag acccaacttc aatataaaat tgatcaatac tgtttctaac tccacttgat
     1201 tggacatcca aaacttttcc caaatcttcg aaaggtccgg tagggttttg tgattttgca
     1261 actccaattc cggggttaga atcaccccat gaagaaaagc tataatacag gtaataatat
     1321 ccgtcattgg aattaaataa aatttgtgga gcccaaagat agcctccaga atgccatgtt
     1381 ggttttgtct ggaaagcgtc agccacatat tcccatttta tcatattttt agattttata
     1441 atggggatca atctgttttt tcctgactcc cattcatttt cagtaccata agcataaaac
     1501 caaccgtcac tggccttaat aaatgaagga tctgccaagt ctggttcaaa aagtggattt
     1561 tgatataacg tatcgggaac gctggtttct gtccaaataa gaacttcctc ttttgaatta
     1621 ttgcaggcaa aaaaagttgc aagcaatatt atgcagaaaa ctgttattgt aaagattaac
     1681 aatttatttt tcataattat ttatttttta tttgtattag aaattacttt aattaaaaca
     1741 gaaaaatttc tgttattcat atccgggatt ctgttttaaa ccttttacca aatttacttc
     1801 tctgtaaggt atggggaaac gattactctt cccaattaca aaattggcaa attcgctatc
     1861 acgattcgta agccatgcaa ttttatccgc tgaatcaaaa aagccccaac gttccaaatc
     1921 aagccatctc caactttcac ccgcaagttc acaggatctt tcgtggataa tttgctcacg
     1981 catttttgct ggatcatttc caatgcctgt gaaaacagtc gaattttcca agtcgggtaa
     2041 gttcactctg cgacgaacct tgtcgatata ttggtaagat tcggaagtac gtcctaattc
     2101 attaagagct tcagcataca taagataaat atcggccaaa cgcactacac gaatattgat
     2161 tccattattt tcatggtctt ctgtagttcg tgtatcgtaa ttggtatatt ttttccaata
     2221 aacnnnnnnn nnnccttagc acgtttaact gatgaattag gatcattttc ccattgtaac
     2281 catgttttac cataaaaaag ttcactcaaa ttatttggat cataataaaa taaagtattc
     2341 ttcaatcgag gatcaacttt tccatcaatt gttctttcct gctccaaatc ggtcttaact
     2401 atattactgg ctgttccgtc atcataggag catccattag caggcaagcc taaaaattta
     2461 gcacgctcgt gactaagttg agtaacccc
//
LOCUS       DAOTKS010000244         2474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_34343_length_2474_cov_4.280695, whole genome shotgun sequence.
ACCESSION   DAOTKS010000244 DAOTKS010000000
VERSION     DAOTKS010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34343_length_2474_cov_4.280695"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="P5236_05325"
     CDS             complement(<1..626)
                     /locus_tag="P5236_05325"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRU63818.1"
                     /translation="MKRYLIYIFCFLFLTLFRLNAAPEKVSENQKEINVREIILSEIT
                     DSYEWHITTWDNKPIFVPLPVIVHSKTSGWHIFMSSKFKEFPVYKGFYIAKEGKYKDK
                     IVELDENGKEIRPLDISLTKTATALIFNCILLTILILSIAHYYKKEGIFPKKGFVILM
                     EMLIMDIYEGVIKQCIEKDYKRYAPYLLTLFFFIFLNNLMGLIPFFPFG"
     gene            complement(623..1018)
                     /locus_tag="P5236_05330"
     CDS             complement(623..1018)
                     /locus_tag="P5236_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63819.1"
                     /translation="MKKNHKIIVIYTLMTLFLGFFFCWLTEKIFQQPIFRFYSFLPIL
                     FYLSGIVLFFVLQKYAQASSIKQVNIFLILKLSKIIVWGIVALIYLLAFKVAIKPFVV
                     VFGAYYLFYLIFETFSFYQIEKQYARQKK"
     gene            complement(1002..1220)
                     /locus_tag="P5236_05335"
     CDS             complement(1002..1220)
                     /locus_tag="P5236_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63820.1"
                     /translation="MSSITVPGILGPFTILEHHAPIISLLVKGVISFKNKDKNEISKL
                     SIEDGLVEVSNNNVIICVELLDENEKKS"
     gene            complement(1265..>2474)
                     /gene="atpD"
                     /locus_tag="P5236_05340"
     CDS             complement(1265..>2474)
                     /gene="atpD"
                     /locus_tag="P5236_05340"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008153109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRU63821.1"
                     /translation="APIHSEPPKFDDLTTNDEILFTGIKVIDLLAPYVKGGKIGLFGG
                     AGVGKTVIIMELINNIAKGHKGFSVFAGVGERTREGNDLLREMIEAGVIRYGEEFNKA
                     MEEGKWDLSKVDYEQLKKSQATLVFGQMNEPPGARFSVALSGLTVAESFRDSEESENR
                     DILLFIDNIFRFTQAGSEVSALLGRLPSAVGYQPTLATEMGLLQERITSTKHGSITSV
                     QAIYVPADDLTDPAPATAFSHLDATTVLSRQIAELGIFPAVDPLDSTSRILDPQIVGE
                     EHYETAQRVKQILQRNKELQDIIAILGMEELSEEDRLTVQRARKVQRFLSQPFFMAEN
                     FTGVPGVMVPIEETIHGFKLILDGMLDDLPEMAFLNVGTIKDAIDKGHKLFRKAHDAN
                     KIFKIESINK"
BASE COUNT          833 a          470 c          386 g          785 t
ORIGIN      
        1 ccaaatggga aaaatggaat caatcccatc aagttgttga gaaaaatgaa aaaaaataga
       61 gtgagcaaat aaggtgcata tcgcttgtaa tctttctcaa tacattgttt tataacccct
      121 tcatatatat ccataataag catctccata agaatcacaa aacccttttt tggaaagatt
      181 ccttcttttt tataataatg agctatactc aaaattaaaa ttgtcagtaa aatacaattg
      241 aaaataagtg cagtagcggt ttttgtcaaa gaaatatcca atggtctgat ttcttttcca
      301 ttttcgtcaa gttccacaat tttatccttg tattttcctt ccttagcaat ataaaatcct
      361 ttataaacag gaaattcttt aaatttagaa gacataaata tgtgccatcc ggatgttttg
      421 ctgtgaacaa ttaccggcag aggaacaaaa attggcttat tgtcccatgt cgtaatgtgc
      481 cattcgtaag aatcggtaat ttcagacaaa ataatctcac gcacattgat ttctttctga
      541 ttttctgaaa ccttctcagg tgcagcattt aaccgaaaca aggttaaaaa taaaaaacaa
      601 aaaatatata ttaaatatct tttcatttct tttgacgagc atattgtttt tcaatctgat
      661 aaaaagaaaa agtttcaaaa atcaaataaa atagataata agctccaaat actactacaa
      721 aaggtttgat tgctacttta aaagctaata gataaataag agctactatc ccccacacaa
      781 ttatttttga aagttttaaa attaaaaata tatttacctg ctttatagaa gatgcctggg
      841 catatttttg taaaacaaaa aataatacta tgcctgataa ataaaacaat atcggcaaaa
      901 aagaataaaa cctaaatatt ggttgttgaa aaattttttc tgtcaaccaa caaaagaaaa
      961 aacctaaaaa caaagtcatt aaagtgtaaa tgacaattat tttatgattt tttttcattt
     1021 tcatcaagta attcaacgca aatgataacg ttattattgc ttacttctac taatccatct
     1081 tcaatagaaa gctttgaaat ttcattttta tccttattct taaaactgat tacacctttt
     1141 accaacaacg aaattatggg agcatgatgt tcaaggatag taaaaggacc taatatacca
     1201 ggtacagtaa tcgatgacac ttctccatta aaaaaaattt ttttaggtgt aacaattttt
     1261 aatttcattt attgatactc tctattttga atattttatt tgcatcatgt gcttttctga
     1321 acaatttatg acctttgtca atggcatcct taatagttcc aacatttaag aaagccattt
     1381 caggaaggtc atcaagcatt ccatctaaaa taagcttaaa tccatgaatc gtttcttcga
     1441 taggaaccat tactcctggc acccctgtaa aattttctgc cataaaaaat ggctgggaaa
     1501 ggaaacgttg cactttccga gctcgctgaa cagtcagacg gtcttcttct gaaagttctt
     1561 ccatacctaa aatagcaata atatcctgta attctttatt tcgttgaaga atttgtttta
     1621 ctcgctgggc tgtttcataa tgttcttcac ctacaatttg ggggtcaaga attcgcgacg
     1681 tagaatccaa aggatcaaca gcaggaaaaa ttcctaattc tgcaatttgc cgacttaata
     1741 cagtggtagc atccaggtgc gaaaaggcag tagctggagc tgggtctgtt aagtcatctg
     1801 ctggtacata tatagcttgt accgaagtaa tagaaccatg tttggtagaa gtaattcgtt
     1861 cctgcaataa tcccatttcc gtagctaagg tgggttgata gcccaccgca gaaggcaagc
     1921 gtcccaacaa agcagaaact tcagaaccgg cctgggtaaa gcggaaaata ttgtcgataa
     1981 acaatagaat atctcgattt tcactttctt cgctgtcgcg gaatgattct gccacagtca
     2041 gtccggataa tgccacagaa aaacgtgctc cagggggttc attcatttgt ccaaagacca
     2101 aagtcgcctg agattttttc agttgctcgt agtcaacttt cgacaaatcc cacttacctt
     2161 cttccatcgc cttattaaat tcttctccat agcgtattac gccagcctca atcatttcgc
     2221 ggagcagatc gtttccttca cgggtacgtt caccgactcc cgcaaaaacc gaaaatccct
     2281 tgtgtccttt ggctatattg ttgataagct ccataatgat aaccgtttta ccaacaccgg
     2341 caccaccaaa gagaccaatt tttcctcctt tcacataagg agctaataaa tctataactt
     2401 tgataccggt aaagagaatt tcatcattgg tggtcagatc atcaaatttt ggcggttcgc
     2461 tgtggatagg ggct
//
LOCUS       DAOTKS010000245         2460 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_34603_length_2460_cov_3.649896, whole genome shotgun sequence.
ACCESSION   DAOTKS010000245 DAOTKS010000000
VERSION     DAOTKS010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34603_length_2460_cov_3.649896"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="P5236_05345"
     CDS             <1..1137
                     /locus_tag="P5236_05345"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007214992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HRU63822.1"
                     /translation="NATEFNLPYFFPMGHPKNGIVHIIGPENGFTQPGMTIVCGDSHT
                     STHGAFGAIAFGIGTSEVEMVLATQCILQTRPKTMRITFKGKLNKGVTAKDLALFMIL
                     KLTTGGATGYFVEYAGEAIENMSMEERMTVCNLSIEMGARGGMIAPDETTFAYVKDRE
                     FAPKGKEWEKKLAYWKTLKTDEGAHFDKEYEFNAEEIQPMITYGTNPGMGISITESIP
                     TIDEVNEASRVSFLKALNYMGFQPGEKLLGKSIDYVFLGSCTNGRIEDFRAFANFVKG
                     KKKADNVTAWLVPGSWKVEQQIREEGLLDILQEAGFEVRQPGCSACLAMNDDKIPAGK
                     YAVSTSNRNFEGRQGPGARTILAGPLVAAAAAITGKIADPREFM"
     gene            1172..1765
                     /gene="leuD"
                     /locus_tag="P5236_05350"
     CDS             1172..1765
                     /gene="leuD"
                     /locus_tag="P5236_05350"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRU63823.1"
                     /translation="MEKFIKLTSTVVPLPIENVDTDQIIPARYLKATDKEGFGNYLFA
                     DWRYNKDGSPKADFVLNDSTYKGSILVAGKNFGSGSSREHAAWAIAGYGFKVIVSSFF
                     ADIFRNNALNNGILPVVVSPEFLNEIFKCVFENPETTLTVDLENQTITNNATGRSETF
                     EINSYKKECLLNGFDDIDYLLSKKDLIEAYEAKKENF"
     gene            1848..>2460
                     /locus_tag="P5236_05355"
     CDS             1848..>2460
                     /locus_tag="P5236_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRU63824.1"
                     /translation="MIEIMDTTLRDGEQTSGVSFSTQEKLSIARMLLEELCVDRIEIA
                     SARVSEGEFETVKRITEWAKRNGYLNKIEILGFVDGKNSLEWIQSAGGQVVNLLCKGS
                     LKHCTYQLKKTPEQHVADIEEVIFYAQQMGMAINIYLEDWSNGIKDSEEYVFYLMDEL
                     KNEKIQRFMLPDTLGILNPDEAFQFCKKMIDRYPHLHFDFHAHN"
BASE COUNT          838 a          337 c          511 g          774 t
ORIGIN      
        1 aatgccactg aatttaattt accatatttt tttccaatgg gacatcccaa aaacggcatt
       61 gttcatatta ttggtcctga aaatggtttt actcaaccag gaatgaccat cgtttgtgga
      121 gatagtcata cttcaactca cggagctttt ggggcaattg cttttggtat tggcaccagt
      181 gaagtagaaa tggtgttggc aactcaatgt attttacaaa ctcgtcccaa aacgatgcga
      241 atcactttca aaggtaaact gaataaaggc gtaacggcaa aagatctggc tctttttatg
      301 attttaaaat taacaacggg aggtgcgaca ggttattttg tagaatatgc gggagaagct
      361 atcgaaaata tgagtatgga agaacggatg acggtttgca atttaagcat agaaatggga
      421 gctcgtggtg gtatgattgc tcctgacgaa accacttttg cttatgtaaa agatagggaa
      481 tttgccccca aaggaaaaga atgggagaaa aaattagctt actggaaaac attgaaaaca
      541 gatgaaggag ctcattttga taaagaatat gaatttaatg cagaagaaat tcaacctatg
      601 attacttatg gcaccaatcc tggtatggga atatccatta ctgaatctat ccctaccata
      661 gatgaagtaa acgaagcaag tcgtgtttct tttctgaaag cattgaatta catgggattt
      721 cagccaggag aaaaattatt ggggaaatca attgattatg tgtttttggg cagttgtacc
      781 aatggaagaa ttgaagattt tcgtgctttt gctaattttg taaaaggtaa aaagaaagct
      841 gataatgtca ctgcatggtt ggttcctgga agctggaaag tggagcaaca aatacgtgaa
      901 gaaggtttgc ttgatatttt acaagaagct ggttttgaag ttcgtcaacc tggttgctca
      961 gcttgtctgg caatgaatga tgataaaatt cctgctggaa agtatgcagt ttcaacttcg
     1021 aatcgtaatt ttgaaggacg tcaaggacca ggagcacgaa ctattctggc tggacctttg
     1081 gtagccgctg ctgctgccat aactggtaaa attgctgatc cacgtgaatt tatgtagttt
     1141 gttattttta aatcattaaa aagaaattaa aatggaaaaa tttataaaat tgacatcaac
     1201 agtagtacca ctgccaatag aaaatgtgga tactgatcaa ataataccag cccgttattt
     1261 aaaagcaact gataaagaag gatttggaaa ttatttgttt gccgactggc gatataataa
     1321 ggatggttcg ccaaaagcag attttgtgtt gaatgattct acttataaag gttctattct
     1381 tgtcgctggt aaaaattttg gttcaggttc aagccgtgaa catgcagcat gggctatcgc
     1441 tggttatggt tttaaagtga ttgtttcgag cttttttgct gatatttttc ggaataatgc
     1501 tttgaacaat ggaattttgc ccgtagtggt ttcaccagaa tttttaaatg aaatttttaa
     1561 atgtgttttt gaaaatcctg aaacgacact caccgttgat ttagaaaatc aaacaattac
     1621 caataatgct actggaaggt cagaaacttt tgaaataaat tcctataaaa aggaatgtct
     1681 tctgaacgga tttgatgata ttgattattt gttaagcaaa aaagatttaa ttgaagctta
     1741 tgaagctaaa aaagaaaatt tttaaagtct tgaataaaaa atcgttttaa acgaataatc
     1801 tctatcatat ttgtttaata aattaaaaga atataaaaaa ttacgttatg atagaaataa
     1861 tggatactac cctgcgtgat ggtgagcaaa catcaggagt atctttttcc actcaggaaa
     1921 aattaagcat tgctcgaatg ttgttagagg aattgtgtgt agatagaatt gaaatcgctt
     1981 cagcacgggt atcagaaggg gaatttgaaa ctgtaaaaag aataaccgaa tgggcaaaaa
     2041 ggaatggtta tctaaacaaa attgaaattc ttggttttgt agatggaaaa aattctttag
     2101 aatggattca aagtgctgga ggacaagttg ttaatttgtt atgcaaaggc tctttgaaac
     2161 attgcaccta tcaattaaag aaaactcctg aacaacacgt tgctgatatt gaggaagtta
     2221 ttttttatgc tcagcaaatg ggtatggcaa ttaatatata tctggaagat tggtcaaatg
     2281 gaataaaaga tagtgaagag tatgtatttt atttgatgga tgaactgaaa aatgaaaaga
     2341 ttcaacgttt tatgttacct gatacgttag gtattcttaa tcccgatgaa gcttttcaat
     2401 tttgtaaaaa aatgatagat cgttatcccc atttgcattt tgattttcat gctcataatg
//
LOCUS       DAOTKS010000246         2438 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_34988_length_2438_cov_4.091062, whole genome shotgun sequence.
ACCESSION   DAOTKS010000246 DAOTKS010000000
VERSION     DAOTKS010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34988_length_2438_cov_4.091062"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..1988
                     /gene="argS"
                     /locus_tag="P5236_05360"
     CDS             198..1988
                     /gene="argS"
                     /locus_tag="P5236_05360"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005850079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRU63825.1"
                     /translation="MNLESAIQSIVTKALKLLYGYEIDAQTIQIQKTKKEFEGDLTVV
                     VFPFAKICKRSPEQIASEIGNFLLKNNDIIESYNAIKGFLNLKISTQYWLKELQKIYQ
                     IPDFGIKKSAEDAPLMMIEYSSPNTNKPLHLGHVRNNLLGYSLAQIQQANGWKVIKTN
                     LVNDRGIHICKSMLAWKKYGKGETPVTSGMKGDHLVGKYYVLFEQKYKEEVEELIKNG
                     LSEEEAKKSAPIILEAQKMLRDWENNDPEVRQLWKMMNEWVYEGFNETYRNLGVDFDR
                     IYYESETYLIGKQMVEEGLQKGIFYRRPDGSVWVDLRDEGLDEKLLLRADGTSVYMTQ
                     DIGTAKLRYDEYPIKKMVYVVGNEQNYHFQALSIILDKLGFEWGRNLVHFSYGMVELP
                     EGKMKSREGKVVDADDLIEEMLQTARETSQELGKLDNCTPEEIENIIRIVGLGALKYF
                     ILKVDPRKNMTFNPEESIDFNGNTGPFIQYTYARIQSVLKKAQEQNVNITDLTDFELP
                     ISEKEKYLIQLLTEFPYIVHQAGNEFSPALIANYLFELGKEYNQFYHDFPILKEEEKI
                     RNFRLILSLSTGNIIKTGMGLLGIEVPERM"
     gene            complement(2044..>2438)
                     /locus_tag="P5236_05365"
     CDS             complement(2044..>2438)
                     /locus_tag="P5236_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRU63826.1"
                     /translation="NHLLGIAFSLNKYAGFENMEVINHINKAKIIFSENFQKDVKPEE
                     IAKQLNMSYSCFRRIFKEYTGFAPAQYLQEIRIQHAKELLTNTNLNIKEIAFESGFEN
                     PEYFFTAFKKKTGYTPLKYRNFTQGKGF"
BASE COUNT          872 a          351 c          451 g          764 t
ORIGIN      
        1 aattgctttt tttaacaagg tacaaacaaa tacctcttcc cagtaatgct tttcttctat
       61 tcgacgagac gaaaaacact tcgttgaaca gaaaaaatta agtaaaaagc ataaaatgaa
      121 gagatagaag aatttacaga aagagggtaa ttcttctttt tttcttaaat ttgcacattg
      181 aatttaaaaa caaaaaaatg aatttagaat ctgccattca atctattgta actaaagctc
      241 ttaaattatt atacggatac gaaatagatg ctcaaaccat tcagattcag aagacaaaaa
      301 aagaatttga aggtgacctg acggttgtag tttttccatt tgccaaaatt tgtaaacgtt
      361 cacctgaaca aatcgcttca gaaattggaa actttttgct aaaaaataat gatattatcg
      421 aatcttataa cgcaataaag ggattcttga atttaaaaat cagcactcaa tactggttaa
      481 aagaattgca aaaaatatat caaattcctg attttgggat aaaaaaatca gctgaagatg
      541 ctcctttaat gatgattgaa tattcatcgc ccaataccaa caagccactt catttggggc
      601 atgtccgtaa taatcttttg ggttatagcc tggctcaaat tcagcaagct aatggctgga
      661 aagtgattaa aaccaatctt gtcaatgaca ggggcattca tatttgtaaa tccatgcttg
      721 cctggaaaaa atatggaaag ggagaaacac ctgtcacttc gggaatgaag ggcgatcatc
      781 tggtggggaa atattatgta cttttcgagc aaaaatacaa agaagaagtt gaagaattga
      841 taaaaaatgg cttgagtgaa gaagaagcaa aaaagtctgc ccctattata ttggaagccc
      901 aaaaaatgtt acgtgattgg gaaaataatg atcccgaagt gcgacaatta tggaaaatga
      961 tgaatgaatg ggtgtatgaa ggatttaatg aaacctacag aaacctcggc gttgatttcg
     1021 atagaattta ttatgaatct gaaacctatc ttattggcaa gcaaatggta gaagaaggct
     1081 tgcagaaagg aattttctat cgtcgtcctg atggctctgt gtgggttgat ttacgcgacg
     1141 aaggattgga cgaaaaatta ttgctaagag ccgatggaac atcagtttac atgactcagg
     1201 acataggaac tgctaagttg agatatgatg aatatcccat aaagaaaatg gtgtatgtgg
     1261 ttgggaatga gcaaaattat cattttcagg cactttcaat tattctggat aagttgggat
     1321 ttgaatgggg cagaaatttg gtgcatttct cttatggaat ggtggaatta cctgaaggaa
     1381 aaatgaaaag cagagaaggc aaagtagttg atgccgatga tttgattgaa gaaatgttac
     1441 aaacagctcg tgaaacttcg caagagttag gtaaattgga taattgtacc ccagaagaaa
     1501 ttgaaaatat tatacgtatt gttgggcttg gtgctttgaa atattttatt cttaaagttg
     1561 atccacgcaa gaatatgaca ttcaatcctg aagagtcaat tgattttaat ggaaatacag
     1621 gaccttttat acaatatact tatgctcgca tccagtcggt tttgaagaaa gcacaagaac
     1681 agaatgtgaa tatcactgat ttaactgatt ttgaacttcc aatttcggaa aaagaaaaat
     1741 atttgataca attgcttacc gaatttcctt atattgttca tcaagcaggc aatgaattca
     1801 gccctgcctt gatcgccaat tatttatttg aattagggaa agaatacaat caattttatc
     1861 atgattttcc gatattgaaa gaggaggaaa aaatacgtaa ttttcgttta atactttctt
     1921 tatcaacagg caacattatt aaaacaggaa tgggactttt aggtatcgaa gttccagaaa
     1981 ggatgtagaa agttttttga ataaaagatt tttcaaaaat gatttaagaa gtaaatatga
     2041 attttaaaat ccttttccct gtgtgaaatt tctgtatttc aatggagtat aacctgtttt
     2101 tttcttaaaa gctgtaaaga aatattctgg attttcaaag ccactttcga aagcaatttc
     2161 ttttatattc aaatttgtat tggtcaataa ttcttttgca tgctgaattc gtatttcctg
     2221 caaatattgt gcaggagcaa atccggtata ttctttaaaa attcgccgaa aacaggaata
     2281 actcatattc aattgtttgg caatctcctc aggttttaca tctttctgaa aattttctga
     2341 aaatataatc tttgccttgt taatgtggtt tattacctcc atattttcaa agccagcata
     2401 cttatttaaa gaaaaagcta ttccaagtaa atgattaa
//
LOCUS       DAOTKS010000247         2435 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_35042_length_2435_cov_3.816387, whole genome shotgun sequence.
ACCESSION   DAOTKS010000247 DAOTKS010000000
VERSION     DAOTKS010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35042_length_2435_cov_3.816387"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2402)
                     /locus_tag="P5236_05370"
     CDS             complement(<1..2402)
                     /locus_tag="P5236_05370"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HRU63827.1"
                     /translation="MIAREIDKRSTEEREFIKNIPQKINYQQFNLSARAQRISHAPAV
                     ASRQYPLTGSPKSLVILVNFSDNNFTISQPQAAFTNLLNQQGYSEDGATGSARDYFRD
                     ASNGVFAPQFDVVGPYTLPNKMEYYGANGSDGYDKNPRQMIIDACKLADQDGLDFSQY
                     DTDHDGFVDNIFVYYAGYNEAEGAPENTIWPHRWTLADTRTKFDGVVIFDYACSSELR
                     SSRGTNMCGIGTFCHEFGHVLGLPDYYNTENDEAYTLSVWNIMDAGAYLNNGKTPPTY
                     AAFDRFYLNWLIPEELKSPQNVTLAALTSSNQAFLISQYGNHNLIGNNPSPVEFFMLE
                     NRQQQSWDAFLPGHGMLITHIYYNASDWENNSPNNNMNAMGYDIVEADGMASEHTRAG
                     DPFPGLSNVTSFSPVLRSGININKPLTDIKEQNGFITFNFIGAYVPIINVGKNNFTSF
                     QTVQGTPTTPQEIKVYGKNLKDNIHISFSENNHFEMKLAAEEITQWRKHIELIPNDSI
                     VDTTTVLIRYNPIVPSYIDKHYETLLLESKNAEIIEISLVGFSTPYLTVPEALEPSEI
                     TTSSYIANWASVDNATGYYLTAYTISEGITILTESFDNGLTVPQDWLITAKILNNTAT
                     YSGKAFPSIQLSNPKEMIQTEKYVIPVDELSFFVGLISGNVKILIEAYDGTQWQIVDT
                     LSLSNSFKGIKSYSFKEVADFIQFRLTYLNGTGSVTIDDVSVSFLKKIEFNAKNKWVT
                     VTSDTIINLFPDSECFYKVRASDKTYLYENITDYSNTISLRTEKDTDARKLRVKIHED
                     NS"
BASE COUNT          784 a          413 c          403 g          835 t
ORIGIN      
        1 atagaattat cttcatgaat tttgactctt aattttcttg catcggtatc tttttcagta
       61 cgtaatgaaa tagtattgga ataatcggta atgttttcat acaaataagt tttgtcgctt
      121 gcccgaactt tataaaaaca ttcgctgtca ggaaacagat ttataatcgt gtctgaagtg
      181 actgtaaccc atttgttttt agcattgaat tcaatttttt tcaaaaaact tactgaaaca
      241 tcatctattg ttactgaacc tgttccattc agataagtaa gtcgaaattg tataaaatca
      301 gctacttcct taaaagaata actttttatt cctttaaacg aattggacaa cgataaagtg
      361 tccactattt gccattgagt tccatcgtat gcttcaatta atatttttac atttccagaa
      421 attaatccaa caaaaaaaga taactcgtca acaggaatca catatttttc tgtttgaatc
      481 atttctttag gattacttaa ctgaattgac gggaaagctt ttcctgaata tgtagcagta
      541 ttatttaata tttttgctgt aatgagccaa tcttgtggta cagtcaagcc gttatcgaaa
      601 ctttctgtta aaatagttat tccttcggaa atcgtataag cagttaaata ataacctgtt
      661 gcattatcaa cagatgccca attagcaata taactacttg tagtgatttc acttggttca
      721 agagcttctg gaacagttag ataaggagtg gaaaatccta ctaaggatat ttctattatt
      781 tcggcatttt ttgattccaa aagcagtgtt tcataatgtt tgtcgatata ggaaggtaca
      841 ataggattat aacgaatcag caccgtcgta gtgtcaacaa ttgaatcatt aggtatgagt
      901 tcaatatgtt ttctccattg ggtgatttct tctgcagcca gcttcatttc gaaatgattg
      961 ttctctgaaa aagaaatatg aatgttatct tttaaatttt ttccataaac ttttatttct
     1021 tgtggagtgg taggagttcc ttgtacagtt tgaaaagaag taaaattatt ttttccaaca
     1081 tttataatag gaacataagc tcctataaaa ttaaaagtaa tgaatccatt ttgttcttta
     1141 atatcggtta atggcttatt aatattaatt cctgaacgta aaacaggaga aaaggaagta
     1201 acattgctca aacccggaaa aggatcacct gctcgagtat gttcggaagc cattccgtcg
     1261 gcttccacaa tatcgtagcc catagcattc atgttattat ttggggaatt gttttcccaa
     1321 tcgctggcat tataataaat gtgtgtaatc aacataccat gtcctggaag aaaagcatcc
     1381 caactttgtt gttgacgatt ttccagcatg aaaaattcca ctggtgatgg attattacct
     1441 attaaattat gatttccata ttgagaaatt aaaaaggctt gattactaga agttaaagct
     1501 gcaagggtaa cattttgtgg tgattttagc tcttctggaa tcaaccaatt caaataaaat
     1561 ctgtcgaatg cagcataagt aggtggtgtt tttccattat tcaaataagc accagcatcc
     1621 ataatgttcc atactgacag agtataagct tcatcatttt cagtgttgta atagtctggt
     1681 aaccctaata catgcccaaa ttcatgacaa aaagttccaa tgccacacat atttgttccc
     1741 ctcgacgaac gaagttcaga agaacaagca taatcaaaga taacaacacc atcaaatttg
     1801 gttcttgtat ctgccaatgt ccatcgatga ggccagatag tattttcagg tgctccttct
     1861 gcttcattgt atccagcata atatacgaaa atattatcta caaaaccatc atgatctgta
     1921 tcatattgac tgaaatctaa gccatcttga tcagctaatt tacaagcgtc tataatcatt
     1981 tgtcgaggat ttttgtcata gccatccgaa ccattagctc cataatactc cattttattg
     2041 ggtaaagtat aaggtcctac tacatcaaat tgtggtgcaa acactccatt ggaagcatca
     2101 cgaaaataat cacgagcaga accagtggct ccatcttcag agtaaccttg ttgatttaat
     2161 aaattagtaa atgcagcttg gggttgtgaa atagtaaaat tattatcaga aaaatttacc
     2221 agaatcacca gcgattttgg actccctgtt aaaggatatt gtcttgaagc aacagcagga
     2281 gcatgtgaaa tgcgttgggc tcgtgcagat aagttaaatt gttgataatt aattttttgt
     2341 ggaatgtttt taataaattc cctttcttca gtagatcgtt tatctatttc acgagctatc
     2401 attttagtaa gggagaattt tcctttttta tttac
//
LOCUS       DAOTKS010000248         2435 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_35048_length_2435_cov_2.607983, whole genome shotgun sequence.
ACCESSION   DAOTKS010000248 DAOTKS010000000
VERSION     DAOTKS010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35048_length_2435_cov_2.607983"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1252)
                     /locus_tag="P5236_05375"
     CDS             complement(<1..1252)
                     /locus_tag="P5236_05375"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRU63828.1"
                     /translation="MKKLFVFFVVTCLIVMVSANEYTLKTIPKPKTLDQHNYVSNPDK
                     ILKDETVYAINNILDSLERKTGVEIAVVVVNSIGGEEIKSFATQLFNEWGVGKAKQDN
                     GLLILFVLDQQKVTFETGYGLEGILPDAICKRIQMQNMIPEFKKGNYDAGFLAAIKEI
                     ISIILHEPVPEKIVEPIHWNEILPIALIVYLVLISITWLWIESSIRKIRKNVKLKTNI
                     ARYVAIKNEKKGIISLMTVLPLIIGFISILIFLQPIFLLLLIPIPLTTLPAAIYANIK
                     MQKIRREPIPCKVCGGQMHILSEKDDDAYLKLSQCFEEQLHSIDYDVFVCDNCKNEAI
                     FLLDKPSAYTKCPKCGTKAFILKDKKIIVSPTYLHPGTERITYHCLYCGYEENQNRRL
                     PRLTHSRNTIIAGSIPGGIFSGSGG"
     gene            complement(1278..1724)
                     /locus_tag="P5236_05380"
     CDS             complement(1278..1724)
                     /locus_tag="P5236_05380"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRU63829.1"
                     /translation="MKKLVFPLCLVALFFLFACEEEKYADWKILNEQWLQAHKSDSGF
                     VCTQSGLCYKVIHQGYDRRPNPYSWVSVQYKGYLIDSTLFERGMYEYYLSNAIQGWIE
                     GITKMNGGGTYIFYIPSSLAYGKDGSGQIPPYSTLIYEITLLSSTY"
BASE COUNT          872 a          401 c          325 g          837 t
ORIGIN      
        1 aacctccact accactaaaa attcctccgg gaatagaacc cgcaataatg gtatttcgtg
       61 aatgggtcaa acgaggaagt cgtcgatttt gattttcttc gtaaccacaa taaagacaat
      121 gatacgtaat acgttctgtt ccaggatgta aataagtagg cgatacaata attttcttat
      181 cttttaaaat aaatgctttt gttccacatt tggggcattt agtataagca cttggtttat
      241 ccagtaaaaa gatagcttca tttttacaat tatcacatac aaaaacatca taatctattg
      301 aatgaagttg ttcttcaaaa cactgagata atttcaaata agcatcatca tctttctcgg
      361 ataaaatatg catttgacca ccacacactt tacaaggaat tggttctctt ctgatttttt
      421 gcatttttat atttgcataa atagctgcag gtaaagttgt taaaggaata ggtatcagta
      481 ataacaaaaa aattggttgt aaaaaaataa gaatacttat aaaacctatt ataagaggta
      541 acactgtcat taaggaaatg attcctttct tttcattttt aatggcaaca taacgagcaa
      601 tgttagtctt tagttttaca ttctttctaa ttttacgaat ggaactttct atccacaacc
      661 aagtaattga tattaatact aaataaacaa ttaaagctat tggtaaaatt tcattccaat
      721 gaataggttc aacaattttt tcaggaacag gttcatgaag aataatagaa attatttcct
      781 ttattgcagc taaaaatccc gcatcataat ttcctttttt gaattcagga atcatatttt
      841 gcatctgaat gcgtttgcaa atagcatctg gtaaaattcc ttccagccca tatcccgttt
      901 cgaaagtaac tttttgttgg tccaaaacaa ataatatcaa taagccattg tcctgttttg
      961 cttttccaac cccccattca ttaaaaagct gagtagcaaa tgattttatt tcttcgcccc
     1021 caattgaatt tacaactact accgcaattt caactcctgt ttttctttca agtgaatcca
     1081 aaatattatt aatggcataa acagtttcgt ctttcagaat tttgtcagga ttactcacat
     1141 agttgtgttg atccagtgtt tttggcttag gaattgtttt taaagtatat tcgttagccg
     1201 aaaccattac tattaaacag gtaacaacaa aaaagacaaa caattttttc attattaaac
     1261 gaaattttta tacgaaatta atacgtagat gaaagaagtg ttatctcata aattaatgtt
     1321 gaataaggag gaatttgtcc agagccatct ttcccataag ctaatgaact agggatataa
     1381 aaaatataag ttccacctcc attcatttta gtaattcctt ctatccatcc ttgaatggca
     1441 ttcgacaaat aatattcata catacctctt tcaaataaag tactatcaat taaataacct
     1501 ttatattgta cagataccca actatatgga tttggacgac gatcgtatcc ctggtgaatg
     1561 actttataac aaagtccgct ttgggtacac acaaaacctg agtcagattt atgagcttgc
     1621 aaccattgtt catttaaaat tttccagtca gcatattttt cttcttcaca ggcaaaaaga
     1681 aaaaaaagag ctaccaaaca aagaggaaaa acaagttttt tcatatcatt tttattctta
     1741 aaaatactca aatttctaga tttttgtgta atttagaatc tagtaaatca acgtatttga
     1801 ttttacagca acatcaaaac aaaagatgtt aaattgaatt tcttcaaaag ttttttattt
     1861 aaatatagta atatccttga aaattttctt gctcctttat agtattctta taaaagacaa
     1921 taaaaaagta aattattttt tttatttcaa aagtgtttgc cgctgtaaat taacaacata
     1981 caaatttatt atacaaatat gaggcaaatt taaggtaaac aataataaaa attagaaaat
     2041 tcatttacaa aaatatgaat gatttttcaa taaacattat aagaaccatg aaaaaattga
     2101 aaggtaagtt ggaatgagtt tttttgaatt ctaccatatt aaattttttt ccattttagc
     2161 acctgacaaa cacctaaata aatattaaaa cgataaaagt tataccaagt tgcttcctaa
     2221 ttataaagaa caattcttgt ctttgctcaa aaaaataaac taactttgaa aaacttttcg
     2281 gagtaaatcc atacattgtt tccttgattg gaaacacaat tgctgaagtg gatattgcat
     2341 cgctgcaact gattgtccat ctgttgccca agccgaactc ggcttttggt caaacggagc
     2401 acttgtcggc tcagttgacg catctgttgt aatac
//
LOCUS       DAOTKS010000249         2431 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_35109_length_2431_cov_4.505471, whole genome shotgun sequence.
ACCESSION   DAOTKS010000249 DAOTKS010000000
VERSION     DAOTKS010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35109_length_2431_cov_4.505471"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2265)
                     /locus_tag="P5236_05385"
     CDS             complement(<1..2265)
                     /locus_tag="P5236_05385"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HRU63830.1"
                     /translation="MKKILMTAILLTTIMLVSYSRTIYVATTGNDSNPGTETSPYLNI
                     QKAIDAAAPGDTIYIRGGTYMLTKRIKIEKAGTADARICLWGYPGERVIIDGSQIVAN
                     STNEFKQARCIYVNHFGDYWHFKNLELCNAKDNGMKLEGSYNIVEKCKFYGNNDTGLQ
                     IGMYKDFSIEETQSFPISGEPQFNPGYTYCKYNIVINCDSWYNCDTKSYSGSDDGKDA
                     DGFAAKLFPGPGTEFHGCRAWQNSDDNWDLYMVYHPILIDNCWSWKAGYDKNGTAFGN
                     GNGFKLGGGGTSGGAAFPQSVGAHVVKNCIAFDCLHKGFDQNNAYEAMYLFNNVSWNN
                     EYNYRFPTIFMYGTMYMRNNIGFKPVTLNHEFLSADKPGSQVPNTAYNSWTTIDGCDP
                     YKDGNKVNGVKVYAKDHSAQFKSLSSTLFLADRQADGSLPENDFCKLVEGSIFINAGE
                     NIENFVPQAHSPGGLTLPPVTILYNDGRADMGAFETGEPTIATLSLISGKADQFVYVG
                     TAIINTVYKWGGAATDVIVRDLPDGLNAIKDMTAKTVTISGIPTVEGTYSIENIGGTN
                     TIVLTGNIRISNIAPATLTITQGHAIQEVFFGEPIETIVFTWGGGATDITYTALPAGL
                     TATKDIENKTLTISGVPTTDGSFTVTTLGGMEGSQVTINCNIIRVLRTKVLTGDWYHI
                     QDPYENRPADLQGNVVTFATGSDSSYPTVWDPTYVESTGSAPSGCTPGAINVERNGGS
                     VTWNLPSLVEMKSNI"
BASE COUNT          780 a          479 c          366 g          806 t
ORIGIN      
        1 aatattagat ttcatttcta ccaagcttgg cagattccat gtaacacttc ctccattcct
       61 ttctacattt atagctcctg gtgtacaacc tgaaggagcc gaacctgtac tttcaacata
      121 ggttggatcc caaacagttg gatatgagga atcagatcct gttgcaaatg ttacaacatt
      181 gccctgtaaa tcagcaggtc tattttcata aggatcctgt atatgatacc agtcgccagt
      241 aagaactttt gttcgtaata cacggatgat attacaatta attgtaactt gtgatccttc
      301 cattccgcct aatgtagtta ctgtgaatga accatcagta gtaggaacac ctgaaatggt
      361 taaagttttg ttctctatgt cttttgttgc agtaagacct gcaggaagag cggtataagt
      421 aatatctgta gctccaccac cccaagtaaa tactatggtt tcaattggct caccgaaaaa
      481 tacttcttgt atagcatgac cttgtgtaat agttaatgta gcaggtgcaa tattagatat
      541 gcggatattg cctgttaaaa caatagtgtt agtacctcct atattttcaa tagaataagt
      601 tccttcaaca gtaggaatac cagaaatggt tactgttttt gcagtcatat cttttatggc
      661 atttaaacca tcaggtaagt ccctaacaat tacatcagta gcagcgccac cccatttata
      721 aactgtattt ataatagcag ttccaacata tacaaattga tctgctttcc ctgaaataag
      781 cgataaagtt gcaatggtag gctctcctgt ttcaaacgct cccatatctg ctcttccgtc
      841 attatacaat attgtaacag gaggtaatgt taatccacca ggactatgtg cttgcggaac
      901 aaagttttca atattttcac ctgcattaat aaatatactt ccttctacta atttacaaaa
      961 atcattttca ggaagactac catctgcctg tctatcagcc aaaaaaagcg tagatgataa
     1021 acttttaaat tgtgcagaat ggtcttttgc gtaaactttt acaccattta ctttattccc
     1081 atctttatat ggatcgcagc catcaatagt tgtccaacta ttatacgccg tattaggtac
     1141 ttgagaaccc ggtttgtctg ccgataaaaa ttcatgatta agtgttacag gtttaaaacc
     1201 aatattattt ctcatataca tggttccata cataaaaatt gtaggaaaac ggtaattgta
     1261 ctcattattc catgatacgt tattaaataa gtacatagct tcatacgcat tgttttgatc
     1321 aaatcccttg tgtaaacaat caaaagcaat acagtttttt accacatggg ctcctaccga
     1381 ttgtggaaaa gcagctcctc cagaagttcc accacctcca agtttaaaac cattaccatt
     1441 cccaaatgct gtaccatttt tatcataccc agctttccaa ctccaacaat tatcaattag
     1501 tatcggatga tacaccatgt acaaatccca attatcatca gaattttgcc atgcacggca
     1561 accgtgaaat tcagttccag gaccaggaaa tagtttagca gcaaatccat cagcgtcttt
     1621 tccatcatct gatccactat atgatttagt atcacaatta taccaagaat cacaattaat
     1681 gacaatatta tacttacaat aagtatagcc cggattaaat tggggctctc cagaaattgg
     1741 aaaagactgc gtttcttcga tagaaaaatc tttatacatt cctatttgaa gtcctgtatc
     1801 gttatttcca tagaatttac acttctccac aatattataa cttccttcca acttcattcc
     1861 attgtcttta gcattgcaca attctaagtt tttaaaatgc cagtaatcac caaaatgatt
     1921 tacgtaaata catcgggctt gtttgaattc atttgtacta ttggcaacaa tctgactacc
     1981 atctataatt acacgttctc caggatatcc ccataaacaa attcttgcat cggctgttcc
     2041 tgctttttct attttaattc gtttagttaa catataagtc cctccacgta tataaatagt
     2101 gtcaccaggt gccgcagcat ctatagcttt ttggatattc aaatagggag atgtttcagt
     2161 tccaggattg gaatcatttc cagtagtcgc aacataaatt gtgcgagaat aacttactaa
     2221 cataatagta gttaataaaa tagcggtcat taaaattttt ttcatggttt tataaattat
     2281 taaaagttaa aaataaattt ctcgtttcta atgtagttaa gaaataaaaa attatgggat
     2341 aattaactaa aatagtgtag agttcgattt gctaacattg ttttgtttcg ttaaagccat
     2401 tcaaaatagc gtaaagattt tataaaagac t
//
LOCUS       DAOTKS010000250         2426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_35196_length_2426_cov_4.917334, whole genome shotgun sequence.
ACCESSION   DAOTKS010000250 DAOTKS010000000
VERSION     DAOTKS010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35196_length_2426_cov_4.917334"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(283..1233)
                     /gene="queG"
                     /locus_tag="P5236_05390"
     CDS             complement(283..1233)
                     /gene="queG"
                     /locus_tag="P5236_05390"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HRU63831.1"
                     /translation="MIKDFIKETAASLGFDACGIARAEELKEDADFLKGWLKAGYHGE
                     MYYMEQNFEKRINPQKLVPDCKTVVVTLLNYYIDCPSPTDAPRIAKYAYSETDYHKVI
                     KQKLRQLENEIKNKFGENCFSKDFQHVFVDSAPILERRWAERAGLGWLGKNTQLIHPG
                     LGSYVFIGILLLNVEVEPDLPISSRCGKCKLCWEACPTKALSENGLDARKCISYLTVE
                     YKKEIPPDFQNCLAGFIAGCDICAEVCPWNKKWAVSHNHDELKPNSHILNWKYENWEH
                     LSPADFEEIFHHSALQRIGFKKLKNNIHFSKKFDSLSKNK"
     gene            complement(1286..>2426)
                     /locus_tag="P5236_05395"
     CDS             complement(1286..>2426)
                     /locus_tag="P5236_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRU63832.1"
                     /translation="MKKIIFLFTISLFLVSCHSHDFFKVQGTVTGAKGEKIYIEHNSL
                     TKVIPLDSVKLSSKESFSFKIHRPEYPDFYRLRLNNQFIVFAVDSCEEITIHADSKSF
                     SKNYTITGSEPSKQIQQLHLSLMKIQEKINTLSSNLPPERKDSFISEIKNDIEKHKEL
                     AKNIIFQNPRSTAAYYALFQKLNNTYLFSPYVKEDRPYCAAVATSYHTFMPEYERSKN
                     LYALVMDAITKERKAIKNEEWEKVLEKNGKGYIDIVLKDSKDKIRKLSELEGKVVLID
                     FSTYETKESIEYIFALRDLYNKYHSRGFEIYQVSLDQNKDRWKQAVENIPWICVRDEN
                     GYQTPYVLSYNVTFIPTNFLMNKKGEIIARDLDFKQLNQMIDNLL"
BASE COUNT          812 a          394 c          359 g          861 t
ORIGIN      
        1 cttcaggaac agagcccaaa attaaattct atattgaagt tcatggaaaa ataaagaaca
       61 ggaatgattt tgatgctgcc gaatctactg ccaacaaaaa aattgaggca gtacaacttt
      121 cgttgggaat ttaaaccgtg ctctcaataa gagatatttt ggcaaaattt tgattaattc
      181 ggaacttcct aatttttacg taatgaaaga aaaattgtag ttgtataaag tttattttta
      241 gttgttttta tcggtttttg cgaaaacaca attttttgaa ctttatttat tttttgagag
      301 agaatcaaac ttttttgaaa aatgaatatt attttttaat tttttaaaac ctattcgttg
      361 tagagcagaa tggtgaaata tttcttcaaa atcagcagga gaaagatgtt cccaattttc
      421 atatttccaa tttaaaatat gagaattggg ctttaattca tcatgattgt gtgaaactgc
      481 ccatttttta ttccagggac aaacttctgc acaaatatca caaccggcaa taaaacctgc
      541 taaacaattt tgaaaatctg gtggtatttc ttttttatat tctacagtaa gataagaaat
      601 acatttgcga gcatccaaac cattttcaga taatgctttt gttggacaag cttcccaaca
      661 taatttacac tttccacaac gagatgaaat aggaagatct ggctcaacct ctacgtttaa
      721 caatagaatt ccaataaaca cataacttcc taagcccgga tgaattaatt gagtattttt
      781 ccctaaccat cctaaacctg ctctttcagc ccaacgtcgt tctagaatag gagcggaatc
      841 aacaaaaaca tgctgaaaat cttttgaaaa acaattttca ccaaatttat tttttatttc
      901 attttccaat tgtcgtaact tttgctttat tactttatga taatcagttt cactataagc
      961 atatttggca attcggggag catcagtagg agaggggcaa tcaatataat agtttaataa
     1021 agttactact accgttttac aatccggtac taatttttga ggattgattc ttttctcaaa
     1081 attttgttcc atataataca tttcgccatg ataaccagct tttaaccaac ccttaagaaa
     1141 atcagcatct tctttcaatt cttctgctcg tgcaattcca caagcatcga aacctaatga
     1201 agcagccgtt tccttaataa aatctttaat catttttcaa taaaataaga gagattatca
     1261 ttttcttttt gtaaaaaaaa cgatattata aaaggttgtc aatcatttgg tttaactgtt
     1321 taaagtccaa atcacgagca ataatttcac cctttttatt catcaagaaa ttggttggga
     1381 taaaagtaac gttgtatgat aatacataag gtgtttgata tccattttca tctctcacac
     1441 aaatccaggg aatattttca acagcttgct tccagcgatc tttgttttga tcaagagaaa
     1501 cttgataaat ttcaaaacct cgtgaatgat atttattata taaatcgcgt aaagcaaaaa
     1561 tatattcaat gctttccttc gtctcataag tggaaaaatc aatcaagacc acttttcctt
     1621 ccagttcgga aagctttcgg attttgtctt ttgagtcttt taaaacgatg tctatatatc
     1681 ctttcccgtt cttttccaaa actttctccc attcttcgtt tttaattgct ttacgttctt
     1741 ttgtaatggc atccattact aaggcatata aatttttgct tctttcatac tcgggcataa
     1801 aagtatgata agaagttgca acagcagcac aataaggtcg atcctctttt acataaggag
     1861 aaaaaagata agtattgttc aatttctgaa aaagagcata ataagcagcc gtagaacgag
     1921 gattttggaa aattatgttt tttgcgagtt ccttatgttt ttcaatgtcg tttttaattt
     1981 cagaaataaa ggaatctttc ctttcaggcg gtaaatttga agaaagagta tttattttct
     2041 cctgaatttt cattaaggac aagtgaagtt gctggatttg cttcgatggt tctgaacctg
     2101 taatggtata attttttgaa aaacttttgc tatcagcatg aattgtaatt tcttcacaag
     2161 aatcaacagc aaaaacaata aattgattgt tcaatcttaa acggtaaaaa tcaggatatt
     2221 caggacgatg aattttaaaa gaaaagcttt cttttgaact taattttacc gaatctaaag
     2281 gaattacttt tgttaaactg ttgtgttcta tatatatttt ttcacctttg gcacctgtca
     2341 ctgttccttg aactttgaaa aaatcatgtg aatggcaaga aaccaagaaa aggcttattg
     2401 taaaaagaaa aataattttt ttcatg
//
LOCUS       DAOTKS010000251         2386 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_35991_length_2386_cov_2.942943, whole genome shotgun sequence.
ACCESSION   DAOTKS010000251 DAOTKS010000000
VERSION     DAOTKS010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35991_length_2386_cov_2.942943"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="P5236_05400"
     CDS             <1..558
                     /locus_tag="P5236_05400"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRU63833.1"
                     /translation="NISRVDSIPYVNSSKNYPMYFFQPSDEIVAFKFTYRKSKGNFAL
                     DDVAVTYRKQDTIYVLKNEPVVGSQFEVDNLNENSTYYYRVRATLGNSVSDFSDVIEV
                     QTTNESGIKHYEDFPFQIIPESDQIRIKGLQGDEQIQIYAISGICLHLTHVNSTEVSI
                     PLKQKGIFIVRILNHNKVFVKKIVK"
     gene            645..1661
                     /locus_tag="P5236_05405"
     CDS             645..1661
                     /locus_tag="P5236_05405"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HRU63834.1"
                     /translation="MPEGYYLSAEGKTGAELKTALHNIIRQHTILNYDTSTSVWWDTY
                     FKTTDWHPEGYFWDMYSNNKRSTYNSSLMNREHCMPRSWWATSDNYSSYDANGDLVNL
                     SPADAEANTKKSNYPLGEVGKNSVTYTNGVVKVGANSFPGYNGTVFEPADEYKGDFAR
                     TYMYMVTCYEDYANNWRGTGTASMLQKNTYPVFKSWAIELLLKWSTNDPVSQKEINRN
                     EGVYQIQKNRNPFIDYPELAEFIWGTKNSEQWQPEIGTSTFVVSPNPASSYVSVQISQ
                     PEQATFIIYDLSGIRLKNGKINSDGTIYMGDLNNGIYLLTIYAQNTRTVTKLIVAHEK
                     LIQN"
     gene            complement(1691..2125)
                     /locus_tag="P5236_05410"
     CDS             complement(1691..2125)
                     /locus_tag="P5236_05410"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRU63835.1"
                     /translation="MENITVNSPVRFAKKEDIDAIHHLLDMEPFKYDNSLPYQRSWIK
                     QLIMNKRCITLVYETEGIIKGFISGEKLVSGSVMIWFCAVKKEYQHRVIGGRLYFEFE
                     NVCKQIGIKGILAYGYKTSVNMLRKLQFFTNEKKYLEFYKVL"
BASE COUNT          810 a          370 c          376 g          830 t
ORIGIN      
        1 aatattagtc gtgttgatag tattccttat gttaacagtt ctaaaaatta tccgatgtat
       61 ttttttcaac cttctgatga aatagttgct tttaaattta cttatcgaaa atcaaaagga
      121 aattttgccc tggatgatgt agcagttact tataggaagc aggataccat ttatgtatta
      181 aaaaacgagc cagtggttgg cagtcaattt gaagttgata atctgaatga aaattccact
      241 tattattatc gagttcgtgc aacgcttgga aattctgttt ctgatttttc ggatgtaata
      301 gaagtacaaa ctacaaatga atcaggaatt aaacattatg aagattttcc ttttcaaatt
      361 atacctgaaa gcgatcaaat aagaataaaa ggattacaag gggatgaaca aattcaaatt
      421 tatgctattt ctggaatttg tttacatctg actcacgtta attctacaga agtaagtatt
      481 ccattgaagc agaaaggaat ttttattgtt cgtatactga atcataataa agtatttgta
      541 aaaaagatag taaaataatt ttttcttatg atttctttaa aatcaaacgc taaaaacata
      601 ctgtttttct ttttgttttt ttttctcaat ttttacaagc tgaaattcct gagggttatt
      661 atttatctgc tgaaggaaaa acaggagctg agttaaaaac cgctttgcac aatattataa
      721 gacaacatac aattttaaat tatgacacca gtacttctgt gtggtgggac acctatttta
      781 aaacaaccga ttggcatcct gaaggctatt tctgggatat gtattctaat aataaacgtt
      841 ctacctacaa ttcttcttta atgaacagag aacattgcat gccacgaagc tggtgggcaa
      901 caagcgataa ttattcttct tatgatgcca atggcgatct ggtaaatctc tcacctgctg
      961 atgccgaagc aaacactaaa aaatcaaatt accctttggg tgaagtaggg aaaaatagtg
     1021 ttacctatac caatggagtt gttaaagttg gagcaaattc ttttcctggt tataatggta
     1081 ctgtttttga acctgcagat gaatacaaag gtgattttgc cagaacttac atgtacatgg
     1141 ttacctgtta tgaagattat gctaacaatt ggagaggaac aggaacagcc agtatgcttc
     1201 aaaaaaatac ctatccagtt ttcaaatcgt gggctatcga gttgttgtta aaatggagca
     1261 cgaatgatcc agttagccaa aaagaaataa ataggaacga aggtgtttat caaatacaaa
     1321 aaaacagaaa tccttttata gattatcctg aacttgctga gtttatttgg ggaacaaaaa
     1381 acagtgagca atggcaaccg gaaataggaa cttcgacttt tgttgtttca cctaatcctg
     1441 cttcttctta tgtgagtgtt caaatcagtc aaccggagca agccactttt ataatttatg
     1501 atttaagtgg aatccgtttg aaaaatggaa aaataaatag tgatggaact atctatatgg
     1561 gtgatttaaa taacggtatt tatttattaa ctatttatgc tcagaacaca agaacagtga
     1621 caaaattaat tgttgctcat gaaaagttaa ttcagaattg ataatttata tttttctttt
     1681 ttaacttttt tcataatact ttgtaaaatt caagatattt tttctcattt gtaaaaaatt
     1741 gaagtttccg aagcatattt actgacgttt tatagccgta agctaaaatt cctttaatac
     1801 caatttgctt gcaaacattt tcaaattcaa agtacaatct accaccaata acacgatgtt
     1861 gatattcctt ttttactgca caaaaccata tcattacact accacttacc aatttttcac
     1921 cactaataaa acctttgatt attccttcgg tttcatacac taaagtaatg catcttttgt
     1981 tcataatcaa ctgttttatc caacttcgtt gatatggtaa gctgttgtca tatttaaaag
     2041 gttccatgtc caacaagtga tgaatagcat cgatatcttc ttttttagca aaacgaactg
     2101 gactattgac agttatattt tccatgaatt gtatttcata attttttgac tcacaagcta
     2161 aattttaaaa aattatcaaa taagcctgag gtatattgat tttttcaagt gtaatcaaaa
     2221 atttttcaga aggaaaaatt ttaaaacttc aataataaaa tttatttcct tttttcaatc
     2281 cattttctgg cattgacaaa agcttctagc catggtgaaa tttcatctct gttttttcta
     2341 tcggctggat aatgtgccca ttgccacgga aatattgctc gttcag
//
LOCUS       DAOTKS010000252         2377 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_36175_length_2377_cov_4.146856, whole genome shotgun sequence.
ACCESSION   DAOTKS010000252 DAOTKS010000000
VERSION     DAOTKS010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36175_length_2377_cov_4.146856"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            378..2288
                     /locus_tag="P5236_05415"
     CDS             378..2288
                     /locus_tag="P5236_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HRU63836.1"
                     /translation="MEDYYKILKEYWGYDEFRPLQSEIIQSIISGKDTLGLMPTGGGK
                     SLTFQVPTMAMEGLCLVITPLIALMKDQVENLKKRNIPAAALYTGMTYEEILMILENA
                     VFEAYKFLYVSPERLATEIFLKKIQQVKVCLIAVDESHCISQWGYDFRPSYLKIADIR
                     QYFPDVPVLALTATATPEVKKDIQEKLHFREENVFQKSFYRSNLAYVVRTIENKNDYL
                     LKILKNVPGTSIVYVRNRKKTKEVADFLLQNGIKAEHFHAGLKNETKDAVQNRWKNNQ
                     TRVIVATNAFGMGIDKPDVRTVIHLDLPDSLEAYFQEAGRAGRDEKKSYAVLLYNESD
                     STKMKKRISETFPKKEAIYKVYESLGNYFQLAVGSGLNAVFPFNLTDFCAKFHLSPLI
                     TYNSLKILQQAGYLEITDEQDSSSRLLFTVDKEELYKLRQSDDEEKLIHMLLRSYTGL
                     FTEFAYINEDLIAERLKWERQKVYEYLVKLAKNRIVQYIPRKKTPFIVFTREREATKY
                     LQLGKEVYEDRRERFISRINSVLNYAQEENICRSQLLLSYFGEKNTRPCGKCDVCLKK
                     KETMMRDKDFVNIRAAIQQILSHQSSPLDMLVKKIPYKEPKVIEVIRFMLDNGQIKEN
                     NQMQLFLTGKEK"
BASE COUNT          828 a          332 c          421 g          796 t
ORIGIN      
        1 tttttctgta aaattcagtg aaaatgaacg gttattacac tataacgtaa tggtcaaaat
       61 gttaattcag tagagacgtc atatatggcg tctctactga aatacgttga ataacagttg
      121 attattcaca atgaataaat actggtaatt atttggttca atgaaacgag gttaattttt
      181 caaccttatt atgctaaaaa ccttcataaa aataaaaaaa caaaccaaac cttattagct
      241 tagtgtataa agtttaaaaa ttgattacat aatatttagt gttattcaat cgagatttaa
      301 aaaagccata atatttattt gcaatagctt tattttagaa atagtttgta taatcacaaa
      361 ttaaaatcca taaataaatg gaagattatt acaagattct taaagaatat tggggatatg
      421 atgaattccg tccattacaa agtgaaatta ttcagagcat aatttcgggg aaagatacgt
      481 tgggcttaat gcctacaggt ggaggaaaat cgttaacctt tcaggtgcct actatggcaa
      541 tggaaggttt atgcctcgtg attactccgc ttattgcttt gatgaaagac caagtggaaa
      601 atttgaaaaa acgaaatatt cctgctgctg ctctttatac tggaatgact tatgaagaaa
      661 tcttaatgat attagaaaat gccgtttttg aagcttataa atttttatat gtttcaccag
      721 aaagacttgc tactgaaatt tttcttaaga aaatccagca agtcaaggtt tgcctgattg
      781 ctgtggatga atcgcactgt atttcacaat ggggttacga ttttcgtcct tcttatttaa
      841 aaattgctga tatccgtcaa tactttcctg atgtgcctgt gcttgctttg acagcaactg
      901 ctacaccaga agtcaaaaaa gatattcaag aaaaattgca ttttcgtgaa gagaacgttt
      961 ttcaaaaaag tttttatcgt tccaatctgg cttatgtggt tcgaacaatt gaaaacaaga
     1021 atgattattt attgaaaatt ttgaaaaatg ttccaggtac ttctattgtt tatgttcgca
     1081 atcggaaaaa aacgaaagaa gttgctgatt ttttacttca aaatgggatt aaagctgaac
     1141 attttcatgc cggtttaaaa aatgaaacca aagatgcagt tcaaaatcgc tggaaaaata
     1201 atcaaactcg tgtgattgtt gctacaaatg cttttggaat gggaattgac aaaccagatg
     1261 ttcgtacagt gattcatctg gatttaccgg attcgctgga agcttatttt caagaagctg
     1321 gcagagctgg aagagatgaa aagaaatctt atgctgtttt attgtataat gagagtgatt
     1381 caacaaaaat gaaaaaacgc atatccgaaa cttttccaaa gaaagaagcc atatataaag
     1441 tttatgagtc attgggaaat tattttcaac tagctgttgg ttcaggattg aatgctgttt
     1501 ttcctttcaa tttgactgat ttttgtgcta aattccattt atcgcctttg attacttata
     1561 atagtttaaa aatacttcaa caagctggtt atttggaaat tacagatgag caggatagtt
     1621 cttctcggtt acttttcacc gtggataagg aagaattgta taagcttcgt caatcagatg
     1681 acgaagaaaa gctaattcat atgcttttac gttcttatac aggtttgttt acagaatttg
     1741 cctatatcaa cgaagatttg atagcggaac gacttaaatg ggaacgtcaa aaagtatatg
     1801 aatatctggt aaaattggct aaaaatagaa ttgttcaata tattccgcgt aaaaaaacgc
     1861 cttttattgt ttttactcgt gaacgtgaag ctactaaata tttgcagttg gggaaggaag
     1921 tttatgaaga tcgacgagaa agatttatat caagaataaa cagtgttttg aattatgctc
     1981 aagaagaaaa tatttgtcgt agtcaacttt tgctttctta ttttggagaa aaaaacacgc
     2041 gtccttgcgg caaatgtgat gtatgcttga aaaagaaaga aacaatgatg agagataaag
     2101 attttgttaa tattcgtgct gctattcaac aaattttatc tcatcaatcc agtcctttag
     2161 acatgttagt caaaaaaatt ccttataaag aacctaaagt gatagaagtc attcgtttta
     2221 tgttagacaa tggtcaaatt aaggagaaca accagatgca attatttcta acggggaaag
     2281 agaaataaaa attaatctgc gtttattttt gctatatata cctgtatatt catatttttt
     2341 aattgcaatt tcatatctct tggaattcct tcatctg
//
LOCUS       DAOTKS010000253         2372 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_36258_length_2372_cov_5.077255, whole genome shotgun sequence.
ACCESSION   DAOTKS010000253 DAOTKS010000000
VERSION     DAOTKS010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36258_length_2372_cov_5.077255"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2264)
                     /gene="ileS"
                     /locus_tag="P5236_05420"
     CDS             complement(<1..2264)
                     /gene="ileS"
                     /locus_tag="P5236_05420"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HRU63837.1"
                     /translation="MKKFTEYSELNLSEINKEILTWWNEQNTFLQSIAIREGKPSFVF
                     YEGPPSANGMPGIHHVMSRTIKDIFCRYKTMQGFQVKRKAGWDTHGLPVELNVEKLLG
                     ITKEDIGKKISIEEYNNVCRREVMRYTQEWEDLTMKMGYWIDMKHPYITYDNRYIETV
                     WWLLKQLYEKKLLYKGYTIQPYSPAAGTGLSTHELNQPGCYRDIKDTSCVAQFKIKEI
                     EKLPFKDKIEGDCYFLAWTTTPWTLPSNTALCVHPNISYALVKTINPYTGKLIYVILA
                     KDLIDKYFSPKNADLSFENYQPGDKNIPYEMLAECSGKELVGIHYEQLIPWVKVEGDA
                     FRVISGDFVTTEEGTGIVHIAPTFGADDDRVAKQNNIPPLLLKDKEGNWQPMVDKTGK
                     FFKLEDLDRDFIAQKINVELYQPYAGRYVKNEYLPEEIQLEDSITTENGINDEATLDI
                     DLCVMLKQQGKIFKIEKYVHSYPHCWRTDKPVLYYPLDSWFVKTTAYRDKMIRLNETI
                     NWKPSSTGTGRFGKWLENLQDWNLSRSRFWGTPLPIWRTEDGSEEICIGSVEELMVEI
                     EKSVAAGLMKKNPYKNFKVGVYTDENYSIENIDLHRPYVDEIILVSESGKPMRRESDL
                     IDVWFDSGSMPFAQLHYPFENKEAIDKHKFYPADFISEGVDQTRGWFFTLHAISTMIK
                     NSVAFKNVVSNGLVLDKNGNKMSKRLGNVIDPFATIEKYGSDPLRWYMMTNSSPWDNL
                     KFDIDGVEEVRRKF"
BASE COUNT          731 a          461 c          369 g          811 t
ORIGIN      
        1 aagaatttcc gacgaacttc ttctactcca tcaatatcaa attttaaatt atcccacggt
       61 gaagaatttg tcatcatgta ccagcgaaga ggatcagaac catatttttc aatcgtcgca
      121 aaaggatcaa taacatttcc taaacgtttt gacattttat ttccattttt gtcaagaacc
      181 aaaccattgg aaaccacgtt tttaaaggcg acagaatttt ttatcatggt actgatggcg
      241 tgcaaggtaa aaaaccatcc acgtgtttga tccactcctt cagagataaa atcggctgga
      301 taaaatttat gtttatcaat ggcttcttta ttttcaaaag gataatgcaa ttgggcaaaa
      361 ggcatcgaac cagaatcgaa ccagacatca attaaatctg attctctccg catcggtttg
      421 ccactttctg acacaagaat aatctcatcg acgtaaggac gatgcaaatc aatattttct
      481 attgaataat tctcatcagt ataaacgccg actttgaaat ttttataagg attttttttc
      541 atcaatcccg cagcaacaga tttttcaatc tctaccatca attcttctac ggagccaatg
      601 caaatttctt ctgatccatc ttctgttcgc caaatgggaa gaggcgttcc ccaaaatcta
      661 cttcgtgaca aattccagtc ctgaagattt tccagccatt ttccaaatcg tcctgttcct
      721 gttgaggaag gtttccaatt gatagtttcg ttaagacgaa tcattttatc ccgataagca
      781 gtagttttta cgaaccaact atccagcgga taatacaaaa caggtttgtc ggtacgccag
      841 caatgtggat aactatgaac atatttttca attttgaaaa ttttgccttg ctgttttaac
      901 atcacacaaa gatcaatatc gagggttgct tcatcattta ttccattttc tgttgtgatg
      961 gaatcttcca attgtatttc ctctggaagg tattcatttt ttacatatct accagcataa
     1021 ggttgataaa gttctacatt gattttctga gctataaaat cacgatccaa atcttccaat
     1081 ttgaaaaatt tacctgtttt atccaccatt ggttgccaat ttccttcttt atctttcaac
     1141 aataaaggag gaatattatt ttgtttagct actctgtcat catctgctcc aaaagtaggg
     1201 gcaatatgaa cgatgcctgt tccttcttcg gttgtaacaa aatcacccga aattactcga
     1261 aaagcatcac cttccacttt tacccacgga ataagttgtt cataatgaat gcctaccaat
     1321 tcttttccag aacattctgc cagcatttca taaggaatat ttttatcacc gggttgataa
     1381 ttttcaaacg ataaatcagc atttttaggt gaaaaatact tatcaatcaa gtccttagct
     1441 aaaatcacat aaatgagctt accagtataa ggattaatag ttttaaccaa agcatacgaa
     1501 atgtttggat gaacgcacaa agctgtatta gaaggcaaag tccagggtgt agtggtccat
     1561 gccaagaaat aacaatctcc ttcaatttta tctttgaaag gtaacttttc aatttctttg
     1621 attttaaact gagctacaca actggtatct tttatgtcgc gataacaacc tggttgattt
     1681 aattcatgcg tactcaatcc tgtgcctgca gcaggagaat atggttgaat agtatatcct
     1741 ttatagagca attttttttc ataaagttgc ttcaacagcc accatactgt ttcaatgtaa
     1801 cgattgtcgt aggtaatgta aggatgtttc atgtcgatcc aataacccat tttcatggta
     1861 agatcttccc attcttgcgt atatctcatt acttcgcggc gacagacatt attgtattct
     1921 tcaatgctga tttttttccc aatatcttct ttcgtaattc ctaatagttt ttccacattc
     1981 aattccacag gaagtccgtg agtatcccaa cccgcttttc tttttacttg aaatccttgc
     2041 attgttttat atctgcagaa aatatcttta atagtacgtg acattacatg atgaatgccc
     2101 ggcataccat tggctgaagg tggaccttca taaaagacaa aagaaggttt tccttcgcgt
     2161 atagcaatgc tttgaagaaa tgtattttgt tcgttccacc atgtaagtat ttctttattg
     2221 atttcagaaa ggtttaactc tgaatattct gtaaattttt tcatgttcga aagcacttga
     2281 tatactattt ttttgaagtt gcaaaattac gaaattaaat tggattaaga agtaataaac
     2341 ttttatgaat caaggtcaac gtataaaaaa tt
//
LOCUS       DAOTKS010000254         2372 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_36260_length_2372_cov_4.296504, whole genome shotgun sequence.
ACCESSION   DAOTKS010000254 DAOTKS010000000
VERSION     DAOTKS010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36260_length_2372_cov_4.296504"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..589
                     /locus_tag="P5236_05425"
     CDS             104..589
                     /locus_tag="P5236_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HRU63838.1"
                     /translation="MGKEIERKYLVKGDFKPWIKNSYSIIQGYLCSVPERTVRIRIKN
                     QERGFITIKGKTEKTGLSRYEWEKEISLQDAQDLLQLCEPGIIEKTRYEVIFNGQRFE
                     VDEFHGENEGLIIAEIELESENTPIQKPDWLGKEVTGDERFYNAQLRKNPYKNWANKD
                     F"
     gene            650..1414
                     /locus_tag="P5236_05430"
     CDS             650..1414
                     /locus_tag="P5236_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRU63839.1"
                     /translation="MKKLFFLMVSCLIFIPLSAQNELFLKKEFIYKGDTLPYRILFPE
                     NYDKTKSYPLVLFLHGAGERGKDNEKQLVHGSSLFTDAQNRENYPAIVVFPQCPENDF
                     WAPVHQEGKDFAFLNTKKPTPSMQLVIRLLNKITKEEAVDKNRIYVLGLSMGGMGTFD
                     LICRQPKKFAAAIPICGGVSLERLKKVRHMPIRIYHGGSDDVVSPEHSRNAFIELKAN
                     GSQKVEYIEFPGVGHNSWKNAFAQPDFLSWLFKQHK"
     gene            1612..1836
                     /locus_tag="P5236_05435"
     CDS             1612..1836
                     /locus_tag="P5236_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRU63840.1"
                     /translation="MAKIKGAVVIDIERCKGCNLCVVACPPRCLSLSKEANSKGYNFS
                     QLTNPEACVGCVACAYVCPDACITVYKMKV"
     gene            1866..>2372
                     /locus_tag="P5236_05440"
     CDS             1866..>2372
                     /locus_tag="P5236_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     beta"
                     /protein_id="HRU63841.1"
                     /translation="MDNKNKQNNEEVLLMKGNEALAHAAVRCGCDAYFGYPITPQSEV
                     METLMELKPWETTGMIVLQAESEVAVINMVYGAAGCGKKAMTSSSSPGVSLMQEGISY
                     MTSAELPAVIVNVMRGGPGLGTIQASQGDYFQAVKGGGHGDYKLITLAPASVQEMADF
                     IGLAFDLAF"
BASE COUNT          825 a          353 c          454 g          740 t
ORIGIN      
        1 caatctattt gcgacagaca gtaataagta tagtaaatct aaaacatact taaatttatt
       61 tcataaattt gcattgttat aatcaagttt ttctgatctt gaaatgggaa aagaaattga
      121 acgcaaatat ttggtaaaag gtgatttcaa accttggatt aaaaatagtt attctattat
      181 tcagggatat ttatgttctg taccagaacg aactgttcgt attcgtatca aaaatcaaga
      241 aaggggattt attaccatca aggggaaaac agaaaaaact ggattaagtc gttatgaatg
      301 ggaaaaagaa atttctttgc aagatgctca ggatttgctt cagctatgcg aaccagggat
      361 tattgaaaaa actcgttacg aagtaatttt taatggtcaa cgttttgaag ttgatgaatt
      421 tcatggagaa aatgaaggac ttatcattgc agaaatagaa ttggaaagtg aaaacactcc
      481 tattcaaaaa cccgattggt taggcaaaga agtaacagga gacgaaagat tttataatgc
      541 ccagttaaga aaaaatcctt acaaaaactg ggcaaataaa gatttttaaa taggtaaagt
      601 tgaccatatt atgatttttt actaacattt aataaaaact ttttgtatga tgaaaaaact
      661 tttcttttta atggtttctt gtctgatttt tattccctta tcagctcaaa atgaattatt
      721 tctgaaaaaa gaatttattt ataaaggtga tacgttgcct tatcgtattt tatttccaga
      781 aaactatgac aaaactaaat cctatccttt ggttcttttc ttacacggag ctggcgaacg
      841 aggaaaagat aatgaaaaac agcttgttca tggatcgtca ctttttaccg atgcccaaaa
      901 cagagaaaat taccctgcaa tagttgtttt tcctcaatgt cctgaaaatg atttttgggc
      961 acctgttcat caggaaggga aagattttgc ttttttaaat accaaaaagc cgacaccttc
     1021 tatgcagctt gttatccgtc ttctgaataa aatcacaaaa gaagaagctg tggataaaaa
     1081 ccgaatatat gtattagggc tttcgatggg aggaatggga acttttgatt taatttgcag
     1141 acaacctaaa aaatttgcag cagctattcc tatttgtgga ggagtgagtt tagaacgact
     1201 taaaaaagtt cgtcatatgc ccatccgcat ttatcatggt ggcagcgatg atgttgtttc
     1261 accagaacat tcacgaaatg cttttattga gctgaaagcc aatggctctc aaaaagtaga
     1321 atatattgaa ttcccgggtg taggacataa tagttggaaa aatgcttttg cacaaccaga
     1381 ttttttaagt tggctattca aacaacataa ataacattga aattgaatag tttagcttgt
     1441 cactttgtta aataactctt accaaaaaag caatattgcc acaattatca ccctgattta
     1501 taaaatttga ctaattttgc aacgaaaaaa tgataagtat taaaagtttt tttaaaacag
     1561 aaaaacttca gaatatatca ttagaaaatt aaaagattaa aagatcaaaa aatggcaaaa
     1621 attaaaggtg ctgttgtaat agacattgaa cggtgtaaag gatgcaattt atgtgtagtt
     1681 gcttgtcctc ctcgatgcct gtctttatca aaagaagcaa attcaaaagg atataatttt
     1741 tctcaactaa ctaatcctga ggcatgcgtg ggttgtgtag cgtgtgctta cgtttgcccc
     1801 gatgcctgta tcactgtata taaaatgaaa gtttaataat ttcttaatgg taagataata
     1861 taaaaatgga taataaaaac aaacaaaata atgaagaggt tctgctaatg aaaggcaatg
     1921 aggcacttgc tcatgctgct gtacgttgtg gctgcgacgc ttattttgga tatcctatca
     1981 ctcctcaatc cgaagttatg gaaacgctca tggaacttaa accatgggaa acaacaggga
     2041 tgatagtttt acaagctgag agtgaagtag ctgttatcaa tatggtatac ggagctgctg
     2101 gttgcggaaa gaaagccatg acttcctctt cgagtcctgg agtaagcctt atgcaagaag
     2161 gaatttcata catgacatct gctgaattac cggctgtaat agttaatgta atgcgcggtg
     2221 gaccaggttt aggaactatt caagcaagtc agggagatta ttttcaggca gtaaaaggtg
     2281 gagggcatgg cgattataaa ctgattacat tggctccagc ttctgtgcag gagatggctg
     2341 atttcattgg tttagcattt gatcttgctt tt
//
LOCUS       DAOTKS010000255         2357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_36557_length_2357_cov_4.092094, whole genome shotgun sequence.
ACCESSION   DAOTKS010000255 DAOTKS010000000
VERSION     DAOTKS010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36557_length_2357_cov_4.092094"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="P5236_05445"
     CDS             <1..321
                     /locus_tag="P5236_05445"
                     /inference="COORDINATES: protein motif:HMM:NF027691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase family protein"
                     /protein_id="HRU63842.1"
                     /translation="NYKNYGIADLLDFYMTGAYSKTLYGSGEWTVQGALQLAQEVTKG
                     DVLVCGGLYGLNFYNAPQDCEKAVYICLTESNGLMFFDIIYLIMYDQWENVKAGINKA
                     LNKE"
     gene            345..2336
                     /locus_tag="P5236_05450"
     CDS             345..2336
                     /locus_tag="P5236_05450"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRU63843.1"
                     /translation="MKLKPIFLSFLLTFVFLSFPLTSQISIVAHRGASAYELENSMAS
                     FKKAFELNADAIELDIWKSKDDSLIVMHDQMTGRTANKNLNISTSTAKELRNLRLKNG
                     EQIPFLQEVLDITPEGKKVVIEIKNKNEKDAKTIISNLSSILKSSGKENDAVIISFHL
                     PMLIEAKKQLPHLKCYYLSSKKNKEDELIEICKKYNLDGLDVHYKILTDDLAQKAKET
                     GLDVWTWTVDDPKIALFVRVNNKVSAITTNCPDIISDILEKNNYQTLYYEQKRSIFKI
                     LPHENNEIIFLGNSITDCGEWPEFFQNSKIKNRGISGDITLGVLARLNDILVAQPSKI
                     FLLIGINDISKNNPDSIILYNYQKIIEEIQEKSPTTQLYIQSILPINNDFKNYPRLQN
                     KEKHILFLNKKLKELCQEKNLIYIDLSPAFCNDKEQLKAEFTNDGLHLMGEGYKMWID
                     ILKPYVNENKSVTNKLFETPYYMSRLKAHRQEKTRANSIIFLGNSITEQGWWSILLKN
                     EDIVNRGIGGDNTYGILHRLPEISQTKPKKIFLMAGINDITAGRDITEIILNFEKIIE
                     ITQAISPQTQLYIQSVLPVNDTSLAYNELKGKNSTIIQLNSQLKKLCEEKNIPYIDIA
                     SLLSDEKGQLKANLTKDGIHLQPEAYIIWTDYLKKMNYL"
BASE COUNT          937 a          338 c          346 g          736 t
ORIGIN      
        1 aattataaaa attatggaat tgctgatttg ttagattttt atatgacagg agcttatagt
       61 aaaacccttt acggttcagg agaatggaca gtgcaaggtg cacttcaact ggctcaagaa
      121 gtgacaaagg gagatgtatt agtatgtggc ggtttgtatg gtttaaattt ttacaatgcc
      181 cctcaagatt gtgaaaaagc tgtttacatt tgtctaacag aatcgaatgg attgatgttt
      241 ttcgacatta tttacttgat tatgtacgat cagtgggaaa atgtaaaagc tggaattaac
      301 aaagctttaa acaaagaata aaataatttt taaatcaaca aaaaatgaaa ttaaaaccaa
      361 tttttttaag ctttctacta acctttgttt ttctttcttt tccccttact tctcaaataa
      421 gcattgttgc tcatcgaggt gcttctgctt acgaacttga aaatagtatg gcttccttta
      481 aaaaagcttt tgaattaaat gccgatgcta tcgaattaga tatatggaaa tctaaagatg
      541 attccttaat agtgatgcat gatcaaatga ctggacgaac agccaacaaa aacttaaaca
      601 tttctacttc tacagcaaaa gaattacgta atttacgact aaaaaacggc gaacaaattc
      661 cttttttaca agaagtactt gacatcactc ctgaaggcaa aaaagtagtt atcgaaatca
      721 aaaacaaaaa tgaaaaagat gcaaaaacaa tcatttcaaa tttaagttct atattaaaaa
      781 gctcagggaa agaaaacgat gctgttatta tttcgtttca tctccctatg ttaatcgaag
      841 ctaaaaaaca actaccacat ttaaaatgct attatttaag tagcaaaaaa aataaagaag
      901 atgaattaat tgaaatttgt aaaaaataca atcttgacgg tttagatgta cattacaaaa
      961 tattgactga cgatttagca caaaaagcga aagaaactgg tttagatgta tggacatgga
     1021 cagtggatga tcctaaaata gccttattcg ttcgagtaaa caataaagta agtgctatta
     1081 ctaccaattg cccagatata atttcggata ttttagaaaa aaacaattac caaacacttt
     1141 actatgaaca aaaaagatct attttcaaaa tacttccaca tgaaaataat gaaattattt
     1201 ttttaggaaa cagcattact gattgtggag aatggcctga gttttttcaa aattcgaaaa
     1261 taaaaaatag aggaatatca ggtgacatta ctttgggagt tttagcacgt ttaaatgata
     1321 tattagttgc tcagccttca aaaatttttc ttttaatagg aataaatgat atatcaaaaa
     1381 ataatcctga ctcaattatt ttgtataatt atcaaaaaat cattgaggaa atacaagaaa
     1441 aaagtccaac cactcaacta tacatccaaa gcattttacc cattaacaat gattttaaaa
     1501 attatccacg acttcaaaac aaagaaaaac atattctctt cttaaataaa aaattaaaag
     1561 aactttgtca agaaaaaaat ctaatttata ttgacctttc cccagcattt tgtaatgaca
     1621 aagaacaatt aaaagcagaa tttacaaatg acggacttca tttgatgggt gaaggatata
     1681 aaatgtggat tgatatatta aaaccttatg ttaatgaaaa taaatctgtt actaataaat
     1741 tatttgaaac tccttattat atgagccgtt taaaagctca tcgtcaggaa aaaacaagag
     1801 ccaatagtat aatatttttg ggaaatagta tcacagagca aggatggtgg tctattttat
     1861 taaaaaatga agacattgtc aacagaggca ttggaggtga taacacttat ggaattttac
     1921 atcgtttgcc tgaaatctca caaacaaaac ctaaaaaaat ttttttaatg gctggaataa
     1981 acgatattac tgctggacgg gatataacag aaataatttt aaattttgaa aagataattg
     2041 aaataacaca agctatttct cctcaaacac aattgtatat tcaaagtgtt ctacctgtaa
     2101 atgatacttc tcttgcttat aatgaattaa aaggtaaaaa ttctaccatt atccagctta
     2161 atagtcaact taaaaaattg tgtgaagaaa aaaatattcc ctatatcgac attgcttctt
     2221 tgctttctga cgaaaaagga caattaaaag caaatttaac caaagatggc atccatcttc
     2281 aaccagaagc ttatattatt tggactgatt atttgaaaaa aatgaattat ttataacacc
     2341 tacagattta tggcaaa
//
LOCUS       DAOTKS010000256         2352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_36671_length_2352_cov_3.169351, whole genome shotgun sequence.
ACCESSION   DAOTKS010000256 DAOTKS010000000
VERSION     DAOTKS010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36671_length_2352_cov_3.169351"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(215..>2352)
                     /locus_tag="P5236_05455"
     CDS             complement(215..>2352)
                     /locus_tag="P5236_05455"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRU63844.1"
                     /translation="LGLMVDGTSFTSKRNIFVTPDGILVIRVQDNGAPSPLQVNLAID
                     SIVRIYNNFGIYNVPHDPWEGSATAREQNTTKGIVVTATRPNTSIASLAVAIESSSSV
                     TVSADNQIVSTSEPNGIVTYYVAPTSSFNPLTPTVPWDHAWNAAYAAKEKGYEALRQE
                     TVAWWSNYFSRSKISVPDENVLKLYVQSLFYEGVYFGNTKIPPGCNGTDIESFAGAIC
                     PEYDLVFSQLALLYSGHIAEAKNIAEWTYNVLPRAKANAVNGITHHNVTRKYDSGAIY
                     TTLMGYDGTLCVQPTEGEGINLYENYPGANASLMALSYLDSSNDNTFTEKALDILKST
                     TYVSLQDLIYNGKTYQCKNMPNTVQQAADLYGYNQCVKRGIADPGWAVYDGKILIPQT
                     TLFGDTLIAGGVGAPAEEGVGDATWLFPLWWYGVVDKQDKKVLPSYINVAKSNTGDYV
                     FNNGTMGIIAAKLGLGNDALSWLQRFQSDNILYDETCFTESKGQLYLTPEIGAHGSYI
                     CNLTQMLLDPDNEHIIDVFPAIPDSWEYRKIGFDSLMVTGGIRITATRDIEGVRLELK
                     NTSLNEINRIVRIAIPRMMDVDNLNTSSMISGKIVNNVSLQPGETKILEYSYKPSKII
                     ASVNNININDDAIKYKIFPNPNSTGQLRIMNGQDVKLIRIYNLKGELVKILIGNSETY
                     FIGKLPQGIYILNMELKTSKHVNHLLLIK"
BASE COUNT          745 a          469 c          362 g          776 t
ORIGIN      
        1 gatcgtccca ttttatgtca tttataacag aaggactacc aacaatacct acaaaatagt
       61 caaaacctcc acgcccattt tttgaaggat cagtcttttt ttgtgcaaaa attaattgac
      121 ttaacaacaa taaaatagtc acataaaata aaattatttt tctcatttca taaaatataa
      181 aaatggcaat agccggatta ataaaatagt tatattattt aattaaaaga agatgattaa
      241 catgtttaga tgtttttagt tccatattca atatatatat cccttgtggt aactttccga
      301 tgaaataagt ctctgaattt ccaattaaga ttttaactaa ttctcccttc aaattataaa
      361 ttctgattaa ctttacatct tggccattca ttattcgaag ctgcccagta ctattaggat
      421 tcgggaatat tttatattta attgcatcat catttatatt tatattatta acagaggcaa
      481 taatttttga tggtttatag ctatattcaa gtattttcgt ttcgcccggt tgtaaagaaa
      541 cattgtttac tattttcccg gaaatcatag atgaggtatt taaattatcc acatccatca
      601 tgcggggaat tgcaattctt actatcctat ttatttcatt taaagatgta ttttttagtt
      661 ctaatcggac tccttctata tccctcgtcg cagttattct gattccccct gtaaccatta
      721 aactatcgaa tcctattttt cgatattccc agctatcagg tattgctggg aaaacatcaa
      781 taatatgttc attatcagga tcaagtaaca tttgagtcaa attacaaata taactaccat
      841 gggctcctat ttctggagtc aaatacaatt gaccttttga ttctgtgaaa caggtttcat
      901 catatagaat attatcagac tggaatcttt gcaaccatga caaagcatca tttcccaatc
      961 ctaattttgc tgcaataatt cccattgtcc cattattaaa tacataatca ccagtatttg
     1021 atttagcaac attaatataa cttggcaaca ctttcttatc ctgtttatca actactccat
     1081 accaccaaag agggaataac catgtagcat ctcctacgcc ttcctcggct ggagccccaa
     1141 cacctcctgc aataagcgta tcaccaaata aggtagtttg aggaatcaat attttaccat
     1201 cgtatacagc ccatcctgga tcagcaatgc cacgttttac acattgatta tacccgtaaa
     1261 gatctgctgc ttgctgaact gtatttggca tgtttttaca ttgatacgtt ttcccgttgt
     1321 atatcaaatc ctgcaaggaa acgtaggtag ttgatttcag aatatccaac gctttctcag
     1381 tgaaagtatt atcattactg gaatcaagat acgaaagcgc cattaatgag gcattagctc
     1441 caggataatt ttcataaaga ttaattccct caccttcagt tggttgaacg caaagtgtgc
     1501 catcatatcc cattaatgtg gtgtaaatag caccactgtc atatttacga gttacattat
     1561 gatgagtgat tccatttaca gcatttgctt tagctcttgg taatacatta tatgtccact
     1621 cagcaatgtt ttttgcttca gcaatatgac cagaataaag taatgccaac tgactgaaaa
     1681 ccaaatcata ttccggacaa atagcaccag caaaactctc aatatcagta ccattacaac
     1741 cgggaggaat ttttgtattt ccaaaataca cgccctcgta aaaaagcgat tgaacgtaaa
     1801 gttttagtac attttcatcg ggaactgata ttttcgaacg actgaaataa ttactccacc
     1861 aagctacagt ttcctgacga agagcttcat atcctttttc tttcgctgca taagctgcat
     1921 tccatgcatg atcccaagga actgtaggag taagtggatt gaaagaagaa gttggtgcta
     1981 catagtaagt tacaatgcca ttgggttcac ttgtactcac aatttggttg tctgcactca
     2041 cagtaacact cgaagaactt tcaatcgcaa cagccagtga agctatgctg gtattaggtc
     2101 gggtagcagt cacaacaatg ccttttgtag tgttttgctc acgagcagtt gccgaacctt
     2161 cccatggatc gtgtggtaca ttgtaaatac caaaattatt ataaatccgt acaatggaat
     2221 ctattgctaa gttaacctgt aatggcgaag gcgctccatt atcctgaact ctaattacca
     2281 atattccgtc gggtgttaca aatatattcc gttttgaagt gaacgaagtt ccatctacca
     2341 taagaccgag at
//
LOCUS       DAOTKS010000257         2342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_36888_length_2342_cov_3.132051, whole genome shotgun sequence.
ACCESSION   DAOTKS010000257 DAOTKS010000000
VERSION     DAOTKS010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36888_length_2342_cov_3.132051"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..1013
                     /locus_tag="P5236_05460"
     CDS             123..1013
                     /locus_tag="P5236_05460"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRU63845.1"
                     /translation="MFYLVYPFFWLLSLLPLNFLYCVAKPLYFFIYYVVRYRRKVVRA
                     NLLHSFPDKSLPDLIKVEKNFYHFFSELLVEIVKLIHMSNEELNKRVTFVDTERFFEL
                     CSQGRYPLLMLGHYGNWEWILSMSLLMPKNTSAFTVYKRLKNKKFDQFMLNLRTKRGA
                     VMVEKRSLLRNMIRWAKEGKLGGYGIIADQSPGGKNIQNWTTFLHQETAILPGAEELA
                     RKFNYPVFYARVSRPKRGYYQCQIIPIALEPQKTSENEISQRFMHLLEEDILSHPQYW
                     LWTHRRWKHQRTNQLPINES"
     gene            1039..2061
                     /locus_tag="P5236_05465"
     CDS             1039..2061
                     /locus_tag="P5236_05465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRU63846.1"
                     /translation="MQVSIVILNWNGEKYLSQFLPILIEKTQLSDVEIVVADNGSTDN
                     SVKLLQKNFPSVKLLLFDKNYGFAGGYNKALNQLQSDYFVLLNSDMEVTDNWLQPMLE
                     YMKEHENVAACQPKIHSYFRRSYFEHAGAAGGFIDPYGFAFCRGRIFGRVEEDRGQYD
                     DIKDIFWASGACMMVRAELFSKVGGFDDEFFAHMEEIDLCWRFNSRGYRVICVPQSVV
                     YHIGGGTLNAEHPHKTYLNFRNNLLLLYKNLPNHQLKNIMQQRTIFDYLAAFQLFISG
                     KFKNAQSVLRARKDFKRMKNRFEGKRKENILYSTVSDLPTIYPKSIVIEYYLKGKKKF
                     SDLYFP"
     gene            complement(2147..>2342)
                     /locus_tag="P5236_05470"
     CDS             complement(2147..>2342)
                     /locus_tag="P5236_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63847.1"
                     /translation="YYPKKCGKNRKKGEKYIVSSIEDPDNVNKRRAEIGLGPISDYVK
                     NWGIIWNLEEYKKEQLQEEK"
BASE COUNT          728 a          342 c          444 g          828 t
ORIGIN      
        1 catttaacga tgatttattc agcggctcat cagacgatga cgatgatgat atgtaaaatg
       61 taaatagttg atatttaaaa aaagctctgc attgcagggc ttttttgttt tttaatgata
      121 ttatgtttta tcttgtttat ccttttttct ggctgctttc gttattgcca ctaaattttt
      181 tgtattgtgt ggcaaagcct ctttattttt ttatttatta tgtagtcaga tacaggcgaa
      241 aagtagttcg agcaaatctt cttcattctt tcccagataa atcattgcct gacctaatca
      301 aggtggaaaa aaatttttat catttttttt ctgaattatt ggtggaaatc gttaagctca
      361 ttcacatgag caatgaagag ttgaataaac gagttacttt tgtggacacc gaacgatttt
      421 ttgaactttg cagtcaaggc aggtatcctc tgttgatgtt ggggcattat ggtaattggg
      481 agtggatatt gagtatgagc ttattaatgc cgaaaaacac ttctgctttt acagtttata
      541 aacgtttgaa gaacaaaaaa tttgatcaat ttatgttaaa tttgcgtacc aaacgagggg
      601 cagtaatggt agaaaaacga agtctattgc gaaacatgat tcgctgggca aaagagggga
      661 aattaggagg ttatggaata attgcggatc aaagtcctgg ggggaaaaat attcaaaact
      721 ggacaacttt tttgcatcaa gaaacagcta ttttgccagg agctgaagaa ttagcacgca
      781 aatttaatta tcctgttttt tatgcaaggg ttagtcgtcc taaacgaggg tattatcaat
      841 gtcagattat tcctatagcc ttagaaccgc aaaaaacttc tgaaaatgaa atttcgcagc
      901 gtttcatgca tttgcttgag gaagatattc tttcccatcc gcaatattgg ttatggacac
      961 acagacgctg gaaacatcaa agaacgaatc agcttccaat aaatgaatct taacttatta
     1021 tttaaaaatt agttgtttat gcaagtttcc attgttattt tgaactggaa tggagaaaaa
     1081 tatttatcgc aatttttgcc tattttaatt gaaaaaacac aattatctga cgtagaaatt
     1141 gtggtggctg ataatggttc aacagataat tctgtaaaat tattgcaaaa aaattttcca
     1201 tcagttaaac tacttttatt tgataaaaat tatggatttg ctgggggata caataaagct
     1261 ttaaatcaac tacaaagcga ttatttcgtc ctattgaatt ctgatatgga agtgactgac
     1321 aattggttac agccgatgct tgaatatatg aaagaacacg aaaatgttgc tgcttgtcaa
     1381 cctaagattc attcttattt tcgtcgttct tattttgagc atgcgggagc tgcgggagga
     1441 ttcatagatc cttatggttt tgctttttgt cgaggacgaa tttttggaag agtagaagaa
     1501 gacagaggac aatatgatga tattaaagat attttttggg cttcaggtgc ctgtatgatg
     1561 gtaagggcag aacttttctc gaaggttggc ggattcgatg atgaattttt tgctcatatg
     1621 gaagaaattg atttatgttg gcggtttaat agtcgaggtt atcgggtaat ttgtgttcca
     1681 caaagtgtgg tttatcatat tgggggtgga acgttaaatg ctgaacatcc gcataaaact
     1741 tatttgaatt tcaggaacaa tttattgctt ttatataaaa atttgcccaa tcatcagtta
     1801 aaaaatatta tgcaacaacg aacaattttt gattatctgg ccgcttttca attgtttata
     1861 tcaggaaaat ttaaaaatgc acaaagtgtt ttgcgtgcaa gaaaagattt taaacgaatg
     1921 aaaaaccgtt ttgaagggaa acgaaaggaa aatattttat attccactgt ttctgatttg
     1981 ccaacgattt atccaaaaag tattgtgata gaatattatt taaaagggaa aaagaaattt
     2041 tccgatcttt attttccctg atttagaata aactttgata ggggacgaat cgcttgcaaa
     2101 cccattataa tcgagatgtt ttatactgct tgtgagcata cgttttttat ttttcctcct
     2161 gtaattgctc ctttttatat tcttccaggt tccaaattat cccccaattt ttaacataat
     2221 ccgaaattgg tcccaatcca atttcagctc ttcttttatt aacattatcc ggatcttcaa
     2281 tggaactgac aatgtatttt tccccttttt tcctgttttt tccgcatttt ttgggatagt
     2341 at
//
LOCUS       DAOTKS010000258         2341 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_36921_length_2341_cov_3.399825, whole genome shotgun sequence.
ACCESSION   DAOTKS010000258 DAOTKS010000000
VERSION     DAOTKS010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36921_length_2341_cov_3.399825"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="P5236_05475"
     CDS             complement(<1..348)
                     /locus_tag="P5236_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63848.1"
                     /translation="MKKLNLLLILCTLTCAFTTSAQDTLDVVPGIGTLNAAIKQYKGG
                     KIYRLKDGYNGYYVLNEIIDNTGFDLTIVGGGKPDANDPKPTMPATLQTSGTGGTPFN
                     FMFNVLSNITLKGI"
     gene            complement(398..1330)
                     /locus_tag="P5236_05480"
     CDS             complement(398..1330)
                     /locus_tag="P5236_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRU63849.1"
                     /translation="MREDKFFLPMLHLRIKPIDWFYTHFSYTHTLRRPDLSMISPFEY
                     YNAQDASSYSYSAGNPNLKTELWKSYDLQLTFHGRKAGLFSITGFKKTVKDKLWSRTY
                     KRIQGEPIPNPVFKDNDLVNMTVYENHPYSILLEGIETEWQTSFGYLPKPFSYLTLSA
                     NYTYTHGKSPNPYTRLYKYKPEGSRYELTGRKDSVVIEPMTGVPEHVFNLTMGIEVKN
                     FKSYLSYQYSSEKILQTHPNDLRLYVISEPYSRLDFNASYGFDFKNKDILEIILRTAN
                     LTNSEDRVRYRGEHRPISVEQYGITVDLGLRYKF"
     gene            complement(1363..>2341)
                     /locus_tag="P5236_05485"
     CDS             complement(1363..>2341)
                     /locus_tag="P5236_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63850.1"
                     /translation="QSFLSTINYYSSSQSKSFNPEGGIATIPIYVNMSKIPKNRTSGI
                     TNNFSGRTTLKFLRSILYYGVSYVYNETDNPGTRTWAYSSKTRADGLSRDSLKVFSPE
                     QVASHFDKILGNLKETELESMSYDQTYAKNWSLTPRIDFEIPIKSNSDLVHGKIQLGA
                     KYRLSHNYFDRNVGIAAVGNNSVFIDFIKNNYNYYWPSGIVELTVAGQENNFLGGSYN
                     FGDKYSFERNDLVFDAWLQHGKETYLAGPTGGLSDDPRYSGFVYDLYSSAMNDLDDIN
                     QYGAGYIMPEINIGKWLMIMPGVRFEYGRTKMKAYSGNEVTRPYSVFKI"
BASE COUNT          765 a          431 c          364 g          781 t
ORIGIN      
        1 gatacccttg agagtaatat tagaaagcac attaaacata aaattaaaag gagtaccacc
       61 cgttccactt gtttgaagtg tagcaggcat tgttggtttg ggatcatttg cgtcaggctt
      121 accccctcct acaattgtaa gatcaaagcc agtgttgtcg ataatttcgt tcaaaacata
      181 ataaccgtta taaccatctt tcaaacgata gattttccct cctttatact gcttgatagc
      241 agcattgaga gtaccaattc cgggaactac atcaagagtg tcttgcgctg atgtagtaaa
      301 tgcacacgtt aacgtgcata aaatcagaag taaatttaat tttttcacga cattgtgatt
      361 taaaagtgaa taataaaact attttgataa tattttttta aaatttatac cttagtccta
      421 aatcaacagt aataccgtat tgttcaacgc taataggacg atgttcacca cgatatcgaa
      481 cacggtcttc actgttagtc aaattagctg ttctaagtat tatttccaat atgtctttgt
      541 ttttaaaatc aaaaccatag gaagcgttaa aatccaatct actataaggc tccgaaataa
      601 catacagcct taaatcattt ggatgagttt gtagtatttt ctctgatgaa tattgataag
      661 ataaataact cttaaagttt ttgacctcaa tacccattgt taaattaaac acatgttctg
      721 gtactcctgt cattggttca atcaccaccg agtcttttct tccagttagc tcataccgag
      781 atccttcagg tttgtattta tataaacgag tatatggatt tggagatttt ccgtgcgtat
      841 aggtataatt agcacttaac gtcaaatacg aaaatggttt tggtaaatat ccgaatgaag
      901 tttgccattc tgtttcaatc ccttctaata aaatggaata agggtgattc tcatatacag
      961 tcatattcac cagatcatta tctttaaaaa ctggatttgg gataggctct ccttgaatac
     1021 gtttgtaagt tcttgaccat aacttatctt taacagtttt cttaaatcct gttatagaaa
     1081 acaagcctgc tttcctccca tgaaaagtta actgtaaatc ataactcttc cataactcag
     1141 tttttaaatt gggattacct gcactgtaag aatagcttga tgcatcctga gcattataat
     1201 attcaaaagg agaaatcatg ctgagatcgg gtcgtcggag agtatgtgta tatgaaaaat
     1261 gagtatagaa ccaatcaatt ggttttatac gtaaatgtaa cattggaaga aaaaatttat
     1321 cttcacgaat agcaatagaa tcttttaagc tgtaagccga atttatatct tgaaaacgga
     1381 gtatggacgt gtaacttcat ttccactgta agctttcatt tttgttctac catattcaaa
     1441 tctcactccg ggcataatca tcaaccattt accaatgtta atttctggca taatatagcc
     1501 agctccatat tgattaatgt cgtccaaatc attcatagcc gatgaataca aatcatatac
     1561 aaatcctgaa tatcttgggt catcactgag gcctcctgtg ggaccggcaa ggtaggtttc
     1621 tttaccgtgt tgcagccacg catcaaaaac taaatcattt cgttcaaaag aatatttatc
     1681 cccgaaatta taacttccac ctaaaaaatt attttcctgc cccgcaactg tcaattctac
     1741 aattcctgaa ggccaataat aattatagtt atttttaata aaatcaataa aaacactatt
     1801 attccctaca gcagctatac ctacattgcg gtcaaagtag ttatgcgaaa gtcgatattt
     1861 agctcccaac tgaattttac catgaactaa gtcagaatta cttttaattg gtatttcaaa
     1921 gtcaatgcgg ggagttaaac tccaattctt ggcataagtc tgatcataac tcatactttc
     1981 caattcggtt tccttcaagt ttcctaaaat cttatcaaag tgactggcaa cctgttcagg
     2041 tgaaaaaact tttagactat cacgagataa gccatctgcc ctagttttag aactatatgc
     2101 ccatgttcta gttccaggat tatctgtttc attgtaaacg taactaacac cataatacaa
     2161 aattgatctt aaaaatttta atgttgtacg tccactaaaa ttattggtaa ttcccgaggt
     2221 cctatttttt ggaattttac tcatatttac atatataggt atagtggcaa tgccaccttc
     2281 gggattaaaa cttttcgatt gacttgaaga gtaataatta atagttgata aaaaagattg
     2341 g
//
LOCUS       DAOTKS010000259         2336 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_37020_length_2336_cov_3.454187, whole genome shotgun sequence.
ACCESSION   DAOTKS010000259 DAOTKS010000000
VERSION     DAOTKS010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37020_length_2336_cov_3.454187"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..399)
                     /locus_tag="P5236_05490"
     CDS             complement(106..399)
                     /locus_tag="P5236_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HRU63851.1"
                     /translation="MNKIVNIALQVLPTSKIVHPYNLVDKAIEVIAASGLKYKVTPFE
                     TVIEGTYDEVMEVVKKAQQACYDAGADSLMTYIKIQSSKNEDVRIADKMEKYE"
     gene            complement(602..1135)
                     /locus_tag="P5236_05495"
     CDS             complement(602..1135)
                     /locus_tag="P5236_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRU63852.1"
                     /translation="MPVKIRLQRHGRKGYAYYHIVVADSRSPRDGKFIERIGSYNPNT
                     NPAIVALKFDRALYWLQVGAQPTDTARNLLSQEGVLLKKHLLGGVKKGAFDEAEAERR
                     FEAWKAFKEAKINMNINEVERKKQAEEKARLAAEAEQNKKKSEELAKKKAAEAAKAQK
                     ENPPEDESSQTNDNPTE"
     gene            complement(1237..2175)
                     /locus_tag="P5236_05500"
     CDS             complement(1237..2175)
                     /locus_tag="P5236_05500"
                     /inference="COORDINATES: protein motif:HMM:NF036727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63853.1"
                     /translation="MEKLSYYVEKLLVRYDYVIVPDLGGFMVQNQSARILQDRIVPPL
                     LTLGFNPLLKDSDGLLAIAIAQEEGISYQSALEWIRKEVELIHFQLQSAKIVSLGKLG
                     FLKRNEIGKLVFVPAERVDFLPANFGMTDIQFFPKKSISSSQNKKVTITLPSASSMRN
                     VAATALVCVLLLVSPRIADTRRSFYTASFPLEMKRSSQTEKIIEKPTSVNSSSQVINT
                     ISELLTSEQEENTATTLKCPYHVIVASFYTEKAAEKFCESLHSIGFEKAHVISPQKNY
                     KVALQSFIEYNDAVEFLHHLHHSEPQFASAWVFIDK"
BASE COUNT          737 a          412 c          356 g          831 t
ORIGIN      
        1 ctcctaaaac aatcatcaaa taaggataga ctattaaagg tgcaacatag ttaagtcctt
       61 gcaatgcaac taaataaata gtatcccaaa actctttttt atatctcatt cgtatttttc
      121 cattttatct gcgattctta cgtcttcatt tttggaactt tgaattttga tataggtcat
      181 caagctatca gcaccggcat catagcaagc ttgctgtgct ttttttacta cttccatcac
      241 ttcgtcataa gttccttcta tgaccgtttc aaaaggcgtt actttgtatt tcagacctga
      301 agctgcgata acttctattg ctttatcaac taaattgtaa gggtgaacaa tttttgaagt
      361 aggaagaact tgaagtgcaa tgtttacaat tttattcatt ttaacaagta attggaaaga
      421 aacttagcaa ttaatcgaaa cgtatcaact ttttaaaaat tgtgtaattt ttataacatt
      481 ttttgaagca aaatttgcat ttatttcgag taaataaaat agaataaaaa caaaaaaacc
      541 caaaattact ttcgggcctt tgctcctaaa aatcagggaa atatcattaa tctttaactc
      601 attattctgt tggattatca tttgtttgtg aagattcatc ttcaggagga ttttcttttt
      661 gagcttttgc agcttcggct gcttttttct ttgctaattc ttccgacttt ttcttgtttt
      721 gttctgcttc ggcagctaaa cgtgcttttt cttcagcctg ttttttcctt tctacttcgt
      781 taatgttcat attaattttt gcttctttga aagctttcca tgcttcaaat cttctttcgg
      841 cttctgcttc atcaaaagct ccttttttta cgcctccaag caaatgtttt tttaacaata
      901 ctccttcttg cgaaagtaga ttgcgtgctg tatctgttgg ctgagctcca acttgcaacc
      961 aatacaaggc acgatcaaat tttaaagcta ctatagccgg attcgtatta ggattgtaag
     1021 aaccaatccg ttcgataaat ttcccatcac gtggggaacg actatctgct acgacaatgt
     1081 gataatatgc gtaacctttg cgtccatgtc gttgtaatct gatttttact ggcattttta
     1141 tacatttttt aaaattatac ctattgcttt ttctcgaaaa gcggtggcaa agttaatgta
     1201 tttcttcgag atacggaaat ttttttcttt taaaaattat ttgtcgataa aaacccacgc
     1261 agaagcaaat tgcggttcgg aatgatgaag gtgatgcaaa aattctactg catcattata
     1321 ttcgataaat gattgaagag caaccttata atttttttga ggggatataa catgtgcttt
     1381 ttcaaagcct atactgtgaa gagattcgca aaatttttca gcagcttttt ctgtataaaa
     1441 acttgctact atgacgtgat aaggacattt aagtgtggta gcagtatttt cttcttgttc
     1501 agaagttaac agctcagaaa tagtatttat cacttgcgaa gaagaattta cagaagttgg
     1561 tttttctatg attttctccg tttgtgatga acgtttcatt tctaatggga aagaagcagt
     1621 ataaaaactt ctccgtgtat cggctatacg aggagatacc aacaataaaa cacaaaccag
     1681 cgcagtagct gccacatttc tcattgaaga agctgatggc aatgttatag taactttctt
     1741 attttgactg gaagaaatag attttttagg aaaaaattga atatcggtca ttccaaaatt
     1801 agcaggtaaa aaatcaactc gttctgctgg aacaaaaact aacttaccta tctcatttcg
     1861 tttcaaaaaa cccagcttgc ctaatgaaac aatttttgct gattgaagct gaaaatgaat
     1921 caattctact tcctttctta tccattctaa agccgactga taactgatcc cttcttcttg
     1981 agcaattgct atggctaata acccatcgct atctttcaac aaaggattaa atcctagtgt
     2041 taacaatgga ggaacaattc tatcttgcaa aattcgagct gattgatttt gaaccataaa
     2101 accgcccaaa tctggtacaa ttacgtaatc ataacgtacc aataattttt caacataata
     2161 actcaatttt tccatattgc aaatataaca agtaagaaaa taaaatccca atattttttg
     2221 tgaaattttt ccacattttg tccattgcaa aaaaacaatt tatctttgta ataagtaaaa
     2281 gtaaaaactt cacaaatgaa aagaacaata ttaattacag gtggagcagg ttttat
//
LOCUS       DAOTKS010000260         2333 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_37077_length_2333_cov_3.033802, whole genome shotgun sequence.
ACCESSION   DAOTKS010000260 DAOTKS010000000
VERSION     DAOTKS010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37077_length_2333_cov_3.033802"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="P5236_05505"
     CDS             complement(<1..214)
                     /locus_tag="P5236_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRU63854.1"
                     /translation="MKNKQTKNISPWAWVPTLYFAEGVPYVVAMTVAVIMYKRLGISN
                     TDIALYTSWLYLPWVIKPFWSPFVDLI"
     gene            complement(539..1861)
                     /locus_tag="P5236_05510"
     CDS             complement(539..1861)
                     /locus_tag="P5236_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HRU63855.1"
                     /translation="MITKEPIIQVGILSSSRIEFILNGAFMTSDGKKVSGKQQVGWKN
                     GMILFDGKTSEELSFQPLDESSTFDLLDVVIGIHFHWERKENQRFKGNLKIIVEHEQL
                     TAINEIKAEDYLVSVISSEMSATSSIELLKAHAVVSRSWLFYPLQKEKKNFSGRNSVE
                     ENSENRHIKWYERGEHEHFDVCADDHCQRYQGITRATSDAVLLAVEATRGEVLRFGQH
                     ICDARFSKCCGGVTETFDNCWAPEEHPYLESIVDNDLPPQGFHLDLSVEANAREWILS
                     SPSAFCNTNDKNVLSQVLNNYDQETMDFYRWKVVYSQKEIAEILCRRSGYDFGEILDL
                     IPLKRGKSGRIVELKIVGTKRTLIVGKELEIRKWLSNTHLYSSAFVVEKEDDRNQVPG
                     KFVLTGAGWGHGVGLCQIGAAVMGEKGYSYDRILLHYFRGAELKRIYE"
BASE COUNT          776 a          477 c          346 g          734 t
ORIGIN      
        1 taattaaatc tacaaatgga ctccaaaatg gctttattac ccaaggcaaa tacagccaac
       61 tggtatacaa ggcaatatcg gtatttgaaa ttcccagccg tttgtacatg atcacagcta
      121 ccgtcatggc aactacataa ggcacgcctt cagcaaaata taaggtggga acccacgccc
      181 acggagaaat gtttttggtt tgtttgtttt tcatgttgat atcggaaaaa tataaagttc
      241 taaatgtgtt ctgataattt taaacaaaca aacttttcat agcattttta attaaagtca
      301 gggcgaggtt gttaatagat tttctcttca tataattgaa aagaaagttt ggggaaaatc
      361 attttttgcc taattactga actaaaaaac ggaactaccg gcagaaacaa aaaatgaaaa
      421 aaactttccc tcaaaaaatt gagaaaagca aaggtaaaaa attctttgcg aacttaaaaa
      481 aaatgtagag tcaaccagag atgttgaatt caagaaaaaa tgtaaaaaaa tttcattctt
      541 attcataaat cctttttagt tcagcgcctc taaaataatg taacaaaatg cgatcataag
      601 aatagccctt ttcacccata acggcagctc caatctgaca tagtcctact ccatgtcccc
      661 aacctgcgcc tgttaataca aattttcctg gcacttgatt ccgatcgtcc tctttttcaa
      721 caacaaaagc agaactatac aaatgagtat tcgacaacca ttttcgtatc tccaattctt
      781 ttcctacaat caacgttcgt ttagtcccta caattttcaa ctccactatc ctacccgatt
      841 ttccacgttt caaaggaatt aaatccaaaa tttctccaaa atcatatccg gaacggcgac
      901 acagtatttc ggcaatttct ttctgactgt aaaccacttt ccaacggtaa aaatccattg
      961 tttcctgatc gtaattgttc aacacctgcg aaaggacatt tttatcattt gtattgcaaa
     1021 aagcagaagg agaacttaaa atccattccc ttgcattcgc ttcgaccgat aaatccaaat
     1081 gaaaaccttg aggaggcaaa tcattgtcga caatactctc caaatatgga tgctcttcag
     1141 gcgcccaaca attgtcaaaa gtctcggtta caccgccaca acatttggaa aatcgagcat
     1201 cgcaaatatg ctgaccaaac ctcaaaactt cgccacgagt agcctcaact gcgagcagaa
     1261 ccgcatcaga tgttgcacgt gtaatacctt gataacgttg acaatggtcg tcggcacaca
     1321 catcaaaatg ttcgtgttcg cctctttcgt accatttaat atgacgattt tcggaatttt
     1381 cttcaactga atttctaccg gaaaaatttt ttttctcttt ttgcaaggga taaaacagcc
     1441 aactccttga aacaacagcg tgggctttca gtaactcaat tgaagaagtt gcgctcattt
     1501 cgctggaaat cacactcacc aaataatctt cagctttaat ttcattaatg gctgttaatt
     1561 gctcatgttc cacaataatt tttaaattcc ctttaaatcg ctggttctct tttctttccc
     1621 aatgaaaatg aataccaatc actacgtcga gtaaatcaaa agttgaggat tcatcaagag
     1681 gttgaaaact caattcttca gaggtttttc catcaaataa tatcattccg tttttccatc
     1741 cgacttgttg ctttcctgaa acttttttgc catccgaagt catgaaagct ccatttagaa
     1801 taaattctat tctgctggaa cttaatatcc ctacctgaat aatcggttct tttgttatca
     1861 cttttatgat tctttaaaaa ttaatttcta agtatttgac aaaaatttaa aaatatcgat
     1921 atttgattga aaaccaacat ttaaaagcac ttttgcgata aagttagagc catatttgaa
     1981 ataacatttt gccattctac cgttgtttag aattctgata ggcttatgat tttcaagtaa
     2041 ataaatcccg attaaataag cgcagaaaaa aacaattgtt actatagcaa cgagtttttc
     2101 aattctctct tttgtatgaa atttacctcg cttaaacatt agtttgaata taacgggaaa
     2161 gacaacccct ttatatacaa tgggcaagac caaaatattg atatttgttt gtccaaactt
     2221 ccagttcgtt ctgtcgatag caatcgagta cggtggatca tgcggtagaa gtgcaaaata
     2281 tcaatttagc aatcaagtct ttgtcaagct tgtaatcttt ttttcattat aac
//
LOCUS       DAOTKS010000261         2331 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_37110_length_2331_cov_4.390598, whole genome shotgun sequence.
ACCESSION   DAOTKS010000261 DAOTKS010000000
VERSION     DAOTKS010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37110_length_2331_cov_4.390598"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="P5236_05515"
     CDS             complement(<1..375)
                     /locus_tag="P5236_05515"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HRU63856.1"
                     /translation="MKRYFIYIAFRGTAYHGWQQQPNGISVQEVLTNAMNMILRSKIN
                     LVGAGRTDTGVHAKSMVAHFDYEGTINEQSFLSHLNCVLPKDIAVFKLIEVKPTAHAR
                     FDAITRKYEYHVVTQKNVFKTDI"
     gene            complement(483..2243)
                     /locus_tag="P5236_05520"
     CDS             complement(483..2243)
                     /locus_tag="P5236_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRU63857.1"
                     /translation="MEITRLFDILDHYLEKYPQQEVALACKRNGEWKKFSIQEYVETT
                     NNISYGMLKLGIQPGDKVCIISNNRPEWNMIDFATMQIGALSVPIYPTISQDDYLYIL
                     NHAEIKMIFVEGKELRNKLMPIFPQIKTLQYIYTFVDQGVNLPYLDQLIELGKENPQP
                     EKLTELKNNIHSSDVATIVYTSGTTGVPKGVMLSHNNIVQQVLHLYDIPSPRDRIALS
                     FLPLCHAYERMLVYLYQYIGLSVYYAENLGTIAENIKEVNPQIMATVPRVLEKMYDKI
                     YLAGKKLPYFSKVIYYWALNLANRYQIENSWWTNFKLKIADKLVYSKWRENIGGDFDI
                     IVSGGSSLRPQLASFFSAIGMPVFEGYGLTETSPVIAVHQKVKNGRKFGTVGLPIPGV
                     EVKIGEQNEILCRGHNVMVGYYKDPELTAQVIDKDGWFHTGDTGKFTPEGQLIITGRL
                     KSLFKTSNGKYINPEIMETKFTESPFIENMIVVGENKKFAAALISPDFVFLRSWCEKH
                     KIPYTSDAEMVEHPEVIKRFQKEIQKYNVHFGDFEQIKRFQLVPDEWTPQNGFLSPNL
                     KIKRNVIEKYYADKIEKLFT"
BASE COUNT          721 a          405 c          370 g          835 t
ORIGIN      
        1 tatatctgtt ttgaaaacat ttttttgagt aactacgtga tattcatatt ttctggtgat
       61 agcatcaaaa cgtgcatggg ctgttggttt tacttcaatg agcttgaaga cggcaatatc
      121 ttttggcaaa acacaattta aatgagatag aaaactttgt tcatttatgg ttccttcata
      181 gtcaaaatga gctaccatcg attttgcatg tacgcctgta tctgtccttc cagcaccaac
      241 aagatttatt ttgctacgca atatcatatt catagcgttt gtcaaaactt cttgcacgga
      301 aataccgttt ggttgttgtt gccaaccgtg ataggctgtg cctctaaaag ctatgtaaat
      361 aaaatagcgt ttcacaatca gagataatct ttgaataatt aaaaatctat tttggcgatt
      421 ttctaaaaga aaggttataa cgttagtaat atttttattt taacttaata aaatgctgat
      481 ttttaagtga ataatttttc aattttatca gcataatatt tttcaataac gtttctcttt
      541 attttcagat taggagataa aaatccattt tgaggtgtcc attcatctgg tactaattga
      601 aaacgtttta tttgttcaaa atcaccaaaa tgaacattgt atttttgaat ctctttttga
      661 aaacgtttta tcacttcagg atgttcaacc atttctgcat cgcttgtata ggggatttta
      721 tgtttttcac accagcttct taaaaagaca aaatcaggag aaattaaagc agccgcaaat
      781 tttttatttt ctcctaccac aatcatgttt tcaataaaag gcgattcggt aaattttgtt
      841 tccataattt caggattgat gtatttgcca ttcgaagttt tgaaaaggct ttttaatctt
      901 cctgtaataa tcagttgacc ttctggcgta aattttcctg tgtctcctgt atgaaaccaa
      961 ccatccttgt ctattacttg agctgttagt tctggatctt tgtaataacc taccattaca
     1021 ttgtgacccc gacaaagaat ttcgttttgt tctccaattt tgacttctac tccaggaatg
     1081 ggcaagccta cggttccaaa ttttcttcca ttttttactt tttgatggac cgcaataaca
     1141 ggggaagttt cagtcaaacc atatccttcg aaaaccggca ttccaatagc agaaaagaaa
     1201 gatgccagtt gtggacgtaa cgaagaacca ccagaaacaa taatatcaaa atcgcctcca
     1261 atattttctc tccattttga ataaaccagt ttgtcagcaa ttttaagttt aaaatttgtc
     1321 caccaactgt tttctatttg gtaacgattg gcaagattta aagcccaata ataaatcact
     1381 ttgctgaaat aaggtaattt tttcccagca agatatattt tatcatacat tttttccaga
     1441 acgcgaggaa cagttgccat tatctgtgga ttcacttcct taatgttttc agctatagtg
     1501 ccaagatttt cagcataata taccgataac cctatatatt ggtataaata taccagcatt
     1561 ctttcgtaag catgacataa tggtaagaaa cttaaagcaa ttctatcgcg aggtgaagga
     1621 atatcataaa gatgcaaaac ctgctgaaca atattattat gtgacagcat tactcccttt
     1681 ggaacacctg tcgtacccga agtatataca attgtggcta catcagaaga atgaatgttg
     1741 ttttttaatt cagtgagttt ttctggttgt ggattttctt ttcctaattc aatcaactgg
     1801 tccagataag gaagattaac accttgatct acaaaagtat aaatgtactg tagtgtttta
     1861 atttgcggga aaataggcat cagtttgttt cgcagttctt ttccttccac aaaaatcatt
     1921 tttatttcag catgattcag aatgtagaga taatcatcct ggctaatagt agggtaaata
     1981 ggaacagaaa gagccccaat ttgcatagtg gcaaaatcaa tcatattcca ttctggtcga
     2041 ttattgctaa taatacaaac tttatcacct ggctgaatac caagttttag cattccatag
     2101 ctaatattgt tagtagtttc tacatattcc tgaatgctga attttttcca ttctccattt
     2161 cgtttgcaag ccaaagctac ttcttgttga ggatattttt ccagataatg atccagaata
     2221 tcaaatagac gagttatttc catgatatta gtaatttatt gaaaaacaat ggttctgttt
     2281 ttataagtaa gaatttttct ttcgatgtgt ttttccactg ctcgagagag t
//
LOCUS       DAOTKS010000262         2325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_37239_length_2325_cov_3.459912, whole genome shotgun sequence.
ACCESSION   DAOTKS010000262 DAOTKS010000000
VERSION     DAOTKS010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37239_length_2325_cov_3.459912"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..1776)
                     /locus_tag="P5236_05525"
     CDS             complement(145..1776)
                     /locus_tag="P5236_05525"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HRU63858.1"
                     /translation="MTKEFKYQEPFPLGEDKTEYYLLTKDYISVSQFEGQEILKVSPE
                     GLTLLARTAFRDCAFLLRPEHQKQVAAILKDPEASENDKYVALTMLRNAEISAKGVLP
                     FCQDTGTALVYAKKGNRVWTDGSDEEALSKGIYETYTQENLRYSQNAPLTMYEEVNTG
                     TNLPGQIDIMADQGEEYKFLFMAKGGGSSNKTFLFQETKALLNPETLEKFLAEKLKLL
                     GTSACPPYHIGFAIGGTSAEACLKTAKLASTKYYDELPTTGNELGRAFRDLELEEKIL
                     KAAQQLGIGAQFGGKYFAHDIRIVRLPRHGASCFVGLAVSCSADRNIKAKINREGIWV
                     EKLEDNPGKYIPEEYRQVGEEGGVKINLNQPMKDILATLTKYPVSTRLSLTGTMVVAR
                     DIAHAKMKEMLDAGHDLPQYMKDHPVYYAGPAKTPKGMPSGSFGPTTAGRMDPYVDLF
                     QAHGGSLVMIAKGNRSQQVVDACKKYGGFYLGSIGGPAAILAQNNIKKVECIDFPELG
                     MEAVWKIEVVDFPAFILIDDKGNDFFKLIKPVVVC"
BASE COUNT          732 a          451 c          348 g          794 t
ORIGIN      
        1 aatatgtttc agatgccttt tttgtttcct gttaaatcag aaaaaatctt ttgttaggac
       61 attttgaaat caaaactatc gtttattacc aagtttcgat ctaaaatgaa atatttaaat
      121 aaggaaagaa ggtcataatt ttttttaaca aaccacaact ggtttgatta acttaaaaaa
      181 atcatttcct ttatcatcaa tcagaataaa ggctggaaaa tcaaccactt caattttcca
      241 aacagcttcc attcctaatt ctgggaaatc aatacattct acttttttaa tgttattttg
      301 tgctaatatt gctgcaggtc caccaattga gcccaaataa aagccaccat attttttaca
      361 agcatccact acttgttggc ttcgattacc ttttgcaatc atcactaaac taccaccatg
      421 tgcttggaaa agatctacat acggatccat acgtccagca gttgtaggtc caaacgatcc
      481 tgaaggcatg cctttagggg tttttgcagg gcccgcataa tataccggat gatctttcat
      541 atattgaggc aaatcatgcc ctgcatcaag catttctttc atcttggcat gagcaatatc
      601 gcgtgccacc accatagtac ctgtaagcga cagacgagta gaaacaggat attttgtcaa
      661 tgtggctaaa atatctttca ttggttgatt cagattaatt ttcacgccac cttcttcgcc
      721 aacctgacga tattcttcag gaatatattt tccgggatta tcttccagct tttctaccca
      781 aattccttct cgattaattt tagccttgat gttcctatcg gcagaacaag aaacagccaa
      841 acccacaaaa caagaagctc catgtcgtgg cagacgaaca atcctaatat catgagcaaa
      901 atattttcct ccaaattgag ctccaattcc taattgttga gctgctttta aaattttttc
      961 ttccagttcc aaatcacgaa aagctctgcc aagttcattg cctgtagttg gaagttcatc
     1021 ataatatttg gttgatgcta atttagcagt tttcaagcaa gcttctgctg aagttccacc
     1081 aatagcaaac ccaatatgat atggtgggca agccgaagtt cctaataatt tcaacttttc
     1141 agccaaaaat ttttccaaag tttcagggtt cagtaaagct ttcgtttcct gaaacaagaa
     1201 agttttattc gaagagccac ctccctttgc cataaaaaga aatttatact cttcgccttg
     1261 atcagccata atatcaattt gcccaggcag attggtaccg gtatttactt cttcatacat
     1321 ggtgagcgga gcattctgcg aataacgcaa attctcctgt gtataggttt cataaattcc
     1381 tttcgaaaga gcttcttcat cactaccatc agtccatacc cgattccctt tttttgcata
     1441 aacaagagca gtacctgtgt cctggcaaaa tggcaaaact cctttggcag aaatttccgc
     1501 attgcgaagc atagtcaaag ccacatattt atcgttttcg ctagcttctg gatctttaag
     1561 aatggccgcc acttgttttt gatgttcagg gcgaagaaga aaagcacaat cgcgaaatgc
     1621 agtgcgagcc aacagagtta atccttcagg tgatactttt aaaatttcct gtccctcaaa
     1681 ctgagatact gatatataat ctttggtaag caaataatat tcagttttat cttcaccaag
     1741 aggaaaaggt tcctgatatt tgaattcttt tgtcatagat taattttctt gattgataaa
     1801 aaataaactt aaaggtccta caaaattaaa gaattttttc tgtaatccat tcttacaatt
     1861 atttttctat ttaaaattgg gtgaataaat tttaaaaaat aatctttgcc cttttattta
     1921 atcataatct aaaaatttat ccaatgaaaa gtgaattttt tgtagatttt tatatgaact
     1981 aaattataaa gttctgagtt ttgagtaaat gaagaaaaaa tctctgtgtc ccatcattgt
     2041 tcggcatagc ctcttggcta cgtcgaacta aaaataaaat ctcttgattt taaccactga
     2101 taaaatcaaa cgttttgcta aacaaatatt tttcataaaa aatcttatca aaaaccttta
     2161 actttcataa tagagaatgt ttcctatagg ttacaacctt attaccttat tggattattt
     2221 tctattttaa tctttcccgt cttttttatt tcgttcagtt tttaataagc taataaggtt
     2281 aacttttttg gaggatttgt tttgttacta aggtttaagc aaaat
//
LOCUS       DAOTKS010000263         2316 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_37435_length_2316_cov_2.443167, whole genome shotgun sequence.
ACCESSION   DAOTKS010000263 DAOTKS010000000
VERSION     DAOTKS010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37435_length_2316_cov_2.443167"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="P5236_05530"
     CDS             complement(<1..588)
                     /locus_tag="P5236_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HRU63859.1"
                     /translation="MLIPEYVFEVSWEVCNKVGGIYTVLSTHAATLQKEYNEKLIFIG
                     PDLWLQKENPLFEEDQNLYTDWKFFHHKQYHLDIRIGRWNVPGKPLTILVDFMPLMPQ
                     RNEIYAQVWLNTGVNSLPAYGDYFECSMFGYAAGMIIESYYRFFHLENQQEVVGHFHE
                     WQTSFGVFYLKQHLPQIATVFTTHATTVGRSIATNN"
     gene            complement(826..1434)
                     /locus_tag="P5236_05535"
     CDS             complement(826..1434)
                     /locus_tag="P5236_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRU63860.1"
                     /translation="MNKFLLVLNAILCIAVIVLFILFFNLKTNKEERKIITTTEALHT
                     NQLPIAYINIDSLLLHYHFAKDANETLLKKQEDSRLTINMKARELQNEMNEFQKKLEN
                     NAFLSRERAEQEQSRLIKKQQDLQKLDAQLSQKLLEEQQKMSEQLRDSINAFLKEFNK
                     DKKYHIILSNTSNDNILYAVDGYDITSEVIKQLNQRCSQQKK"
BASE COUNT          815 a          333 c          293 g          875 t
ORIGIN      
        1 attgtttgtt gctatggaac gtccaacggt tgtggcatga gtagtaaaaa cagttgctat
       61 ctgaggcaaa tgctgtttca gataaaaaac accaaaagaa gtctgccatt cgtgaaaatg
      121 acctactacc tcctgttgat tttctaaatg aaagaaacgg taataacttt caataatcat
      181 ccctgcagca tatccaaaca ttgaacattc aaaataatcg ccataagctg gaagtgaatt
      241 tactcctgta ttcagccaga cttgtgcata aatttcattc ctttggggca tcaatggcat
      301 aaaatcaacc aagatagtta atggttttcc aggaacattc cagcgtccaa ttctaatgtc
      361 taaatggtac tgtttatgat gaaaaaattt ccaatcagta tataaatttt gatcttcttc
      421 aaaaagtgga ttttcttttt gaagccacaa gtcaggacca ataaatatta atttctcatt
      481 gtattccttt tgtaaggtag cagcatgagt tgataatact gtataaattc ctccaacttt
      541 gttacagact tcccaactta cttcaaaaac atattcaggt atcaacattt gattagaaca
      601 tttttattta aaaaaattaa gcacaaattt aatatatcag tacgtgaaaa aatgggaaat
      661 aattgaaaaa aataacaaaa attaggcatt tacttcaaga aaatataaat catcagaaat
      721 tttttacttc aatttttttc atatttttca caaaaaaatt tctcctttaa aaagaggaga
      781 aattttaaac gtaaatatca agtgctattt taataattcc tcttattatt ttttttgctg
      841 actacaacgt tgatttaatt gtttaataac ttctgaagta atatcatatc catcaactgc
      901 atacaaaata ttatcatttg aagtattact caaaataata tgatattttt tgtctttatt
      961 aaattctttt aaaaaagcat tgatgctatc acgtaattgt tcactcattt tttgttgttc
     1021 ttccaacaat ttttgtgata attgggcgtc taatttttgc aaatcctgtt gtttttttat
     1081 taaccttgat tgttcttgtt cggcacgttc gcgacttaaa aaagcattat tttccaattt
     1141 tttttgaaat tcattcattt cattctgaag ctcacgagct ttcatattta tcgtcaatcg
     1201 agaatcttct tgctttttaa gtaaagtttc attagcatct tttgcaaaat gataatgaag
     1261 taacaaagaa tctatattta tataagcaat gggtaattgg tttgtatgaa gagcttcagt
     1321 agttgttatt atttttcttt cttctttatt tgtctttaaa ttaaaaaata aaataaataa
     1381 cacaattacc gcaatgcata aaatagcatt taaaacgagt aaaaatttat tcatatattt
     1441 ctaaattcta ttgttattat tattaatgaa tttgttatta gctttttata aattcttgaa
     1501 tctcaattat tctaaattga tttttgttta cctactttta aaaaacaaca catcctattt
     1561 tttccttact aaaagttgat ctattttcaa ccacattttc aattgtagaa aaaatcattg
     1621 taaaaaatga tataaattac tttcaggaat aatttctttt attttctaaa tcattttata
     1681 taattttttc tcactgagaa gaaataattt ttgaactatc aagtacattc ctcatttttc
     1741 ttatgcaaag ataaattatt tagaatataa tgttactttt tgctttgttt ttttagcaaa
     1801 atgaatattt tgttttattt tttaacacaa gttttaatag ctcaaatcat aatcttagga
     1861 taaattgttc ccgtgtttaa gagcatattc gcttgaagaa acaccgtaaa actctttaaa
     1921 agagtttgaa aaatgtgata aatttgaaaa tccaacagaa taggcaactt cggtttaaaa
     1981 atggggcgat tatcttgaca aagaattaac atttcgaata gaagcaatca tagcagattc
     2041 cgcaaatgac tgggaatatg aaagtggaac aaaagtagtt atactatctt tatccatatt
     2101 gttgatttgc agggaattat tactacgatt cgatgtgata ggcagcattt tcaattccct
     2161 ctttgtgatt tggatatttt gggtaaaaac aaacgaaaca ttcaattaat tgaagattac
     2221 caagtatagt ttgggaataa gccgttttaa aaaaatgctt tcccctaata attcaatttt
     2281 ttataaaaat ccttttacaa tcaaatactc aaaata
//
LOCUS       DAOTKS010000264         2311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_37522_length_2311_cov_4.305408, whole genome shotgun sequence.
ACCESSION   DAOTKS010000264 DAOTKS010000000
VERSION     DAOTKS010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37522_length_2311_cov_4.305408"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="P5236_05540"
     CDS             <1..682
                     /locus_tag="P5236_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRU63861.1"
                     /translation="IRKGPIFANLILADEINRAPAKVQSALLEAMQERQVTIGETTFK
                     LEEPFLVMATQNPIEQEGTYPLPEAQVDRFMLKVVINYPKKEEEKLIIRENLEKNLPS
                     VNPILSPKDIIEARDIVRQVYIDEKIEHYIIDIVFATRYPQEYGLDLLKEMIAFGASP
                     RASINLALASRAYAFIKRRGYVIPEDVRSVCFDVLRHRIGLSYEAEANNMTSEEIITE
                     ILNTIEVP"
     gene            739..1617
                     /locus_tag="P5236_05545"
     CDS             739..1617
                     /locus_tag="P5236_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRU63862.1"
                     /translation="METSEIIKKVRRIEIKSRGLSQNIFAGAYHSVFKGRGMTFSEVR
                     EYQYGDDIRNIDWNVTARFAHPYVKIFEEERELTVMLLIDVSGSSEFGSISRMKKDII
                     TEIAATLAFSTIQNHDKIGVIFFSDKIEKFIPPKKGRKHVLYIIRELVDFEPESNKTN
                     IAAALRYFTNAIKKRSTAFLISDFIDEDFENELTIANRKHDVIALQIYDIRETEIPNV
                     GLIKLKDAETGDRIWLDASDKNLRTFYKHKWEENQLKLERTFTKAKVDAVSISTSDDY
                     VKALLKLFRMRAKIAS"
     gene            1644..>2311
                     /locus_tag="P5236_05550"
     CDS             1644..>2311
                     /locus_tag="P5236_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63863.1"
                     /translation="MRKFLLTGIISSLIAINLNAQKITVDARLDSTVLWLGDQVHLIF
                     EVTQGKNQKVMVPLFSDTIVNGLEIVEPVKRDTVKINDNSIKVNHRYLLTSFEDTLYY
                     IPSFPFIVGKDTVWSKSLSLKVIQPFQIDTASHQIADIKPVFQPKYDWSGLIKKILLV
                     LIFVVLLGLIYFFVRKYIQKKPILEPVSKEPPLPPHIIALNLLDNIRNEKLWQKGKYK
                     EYYS"
BASE COUNT          786 a          343 c          416 g          766 t
ORIGIN      
        1 cattcggaaa ggtcctattt ttgctaattt gattctggca gatgaaatta accgtgctcc
       61 tgccaaagtt caaagtgctt tgttagaagc catgcaagaa cgtcaagtaa ccataggtga
      121 aactactttc aaactggaag aaccttttct ggtaatggct acccaaaatc ctatcgaaca
      181 ggaaggaact tatcctcttc ctgaagctca agtggataga tttatgctaa aagtagtgat
      241 taactatcca aaaaaagaag aggaaaaatt gattatcaga gaaaacttag agaaaaacct
      301 gccttccgtc aatcctattt tatcaccaaa agatattatc gaagctcgtg atattgttcg
      361 gcaagtttat attgatgaaa aaattgaaca ttatattatt gatattgttt ttgctactcg
      421 ttatccacag gaatatggac ttgatctgtt gaaagaaatg attgcttttg gggcttcacc
      481 acgtgcttct attaacttgg ctctggcttc aagagcttac gcttttatca aacgtcgtgg
      541 ttacgtcatt cctgaagatg ttcgctctgt ttgctttgat gtactgcgtc accgaattgg
      601 cttgagttat gaagccgaag ctaataacat gacctctgaa gaaattatca cagaaatttt
      661 aaatactata gaagtgcctt gattttcaat gatttatcta acaggtgtta atgtttttag
      721 tttatattga taaaatctat ggaaacaagt gaaattataa aaaaagttcg cagaattgag
      781 attaaatctc gaggtttatc tcaaaatata tttgctggag catatcactc tgtttttaaa
      841 ggtagaggta tgaccttttc agaagtacgt gaatatcaat atggtgatga tatcagaaat
      901 atagattgga atgtaacagc ccgttttgct catccttatg tgaaaatttt tgaagaagaa
      961 cgtgaactga cagtaatgtt actaattgat gtttcaggta gtagtgaatt tggtagcatt
     1021 tccagaatga aaaaggatat tattactgaa atagctgcga cgttggcttt ttcgacgatt
     1081 caaaatcatg ataagatagg ggtcattttc ttttctgata aaatagaaaa atttattccg
     1141 cctaaaaagg gacgaaagca tgtattgtat attattcggg aattggttga ttttgaacct
     1201 gaaagtaaca aaaccaatat tgctgcagct ttgcgttatt ttactaatgc cattaaaaaa
     1261 agatcgaccg cttttttgat ttctgatttt atcgatgaag attttgaaaa tgaattgacc
     1321 attgccaatc gtaaacatga tgtaattgct ttacaaattt atgatattcg agaaacagaa
     1381 atacccaatg taggattgat aaaattgaaa gatgcagaaa caggggatcg tatttggctt
     1441 gacgcatcgg ataaaaatct tcgtactttt tataaacata aatgggaaga gaatcaatta
     1501 aaattggaaa gaacatttac aaaagctaaa gtggatgcag tttcaataag tacctctgac
     1561 gactatgtaa aagctttgtt aaaattattc agaatgcgag caaaaatagc aagctgattt
     1621 tttcttactt atttaaaaaa attatgcgaa aatttcttct aacaggaatc ataagttctt
     1681 taatagccat taacttgaac gcacaaaaaa taactgtgga tgcacgattg gattccactg
     1741 ttttatggct tggcgaccag gttcatctta tttttgaagt gacgcaggga aaaaatcaga
     1801 aagtaatggt tcctttattt tctgatacga ttgtaaatgg attggaaatt gtagaacctg
     1861 ttaaaaggga tacggttaaa attaatgata atagcatcaa agtaaatcat cgttatctat
     1921 taacttcctt tgaagatacg ttgtactata ttccttcttt tccttttatt gttgggaaag
     1981 atacggtctg gtcaaaatca ttgtcgttaa aagtaattca accctttcaa attgacactg
     2041 cttctcacca aattgcagat attaaacccg tatttcagcc aaagtatgat tggtcaggtt
     2101 tgataaaaaa aattttactt gtcttaattt ttgttgtttt attaggttta atttattttt
     2161 ttgtcagaaa atatatccag aaaaaaccaa ttttagaacc agtttccaaa gaacctcctc
     2221 tgcctccgca tattatcgct ttaaatttat tggacaatat tagaaatgaa aaactttggc
     2281 agaaaggcaa atataaggaa tattacagtg a
//
LOCUS       DAOTKS010000265         2304 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_37668_length_2304_cov_3.947977, whole genome shotgun sequence.
ACCESSION   DAOTKS010000265 DAOTKS010000000
VERSION     DAOTKS010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37668_length_2304_cov_3.947977"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2093)
                     /locus_tag="P5236_05555"
     CDS             complement(<1..2093)
                     /locus_tag="P5236_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRU63864.1"
                     /translation="MEYNFKEIEVKWQNYWNENNIYKTEIDLTKPKYYVLDMFPYPSG
                     AGLHVGHPLGYIASDIYSRYKRMKGFNVLHPMGFDAFGLPAEQYAIQTGQHPAITTKK
                     NIERYREQLNKIGFCYDWSREIRTCDPDYYKWTQWAFIQMFKHYYDEKLQKAQPITLL
                     IEHFEKYGTENLTAVGTIELNFTADEWKAMSEKAQQEILLNYRIAYLADTKVNWCPAL
                     GTVLANDEVSEGVSIRGGYPVEQRVMRQWCLRVSAYAQRLLDGLEQIDWSESLKETQR
                     NWIGRSEGAELRFTIKGKNVSFDVFTTRPDTIFGVTFMVLAPESELVKICTTPEQRKE
                     VEEYLEATKRRTERERIADRQVTGVFTGSYAIHPITGKEIPIWISDYVLAGYGTGAIM
                     AVPAHDSRDYAFAKHFNLPIVPLIEGCDVSNESFDAKEGIMINSEFLNGLSVPEAIEK
                     IKKYITKKGIGRVKVNYRIRDAIFSRQRYWGEPFPVYYKDGMPYVLDEKELPLVLPEI
                     DKYLPTENGEPPLARAKNWKTKEGYPLEVCTMPGFAGSSAYYLRYMDPHNDEALVSRE
                     ADEYWRDVDLYLGGTEHATGHLIYSRYWNKFLFDIGVVCEEEPFKKLINQGMILGRSN
                     FVYRIKDTNTFVSYHLKDQYDTTPIHVDINLVDNDILDIEKFRNWNPEYKNAEFILED
                     GKYICGWAIEKMSKS"
BASE COUNT          712 a          457 c          372 g          763 t
ORIGIN      
        1 attgatttag acattttttc aattgcccag ccgcagatat atttgccatc ttccaaaata
       61 aattcagcat ttttatattc aggattccag ttcctgaatt tttctatgtc gagtatatca
      121 ttgtccacca aattgatatc tacatgaata ggagttgtat catattgatc tttaagatga
      181 taagaaacaa atgtattggt gtccttaatt cgatagacaa aattacttct tcccaaaatc
      241 attccctgat taatcaattt cttgaaaggt tcttcttcgc aaacaacacc aatgtcgaat
      301 aaaaatttat tccagtatcg gctataaatc aaatgaccag tagcatgttc agttccacca
      361 agatataaat ccacgtctcg ccagtattca tctgcttcac gacttaccaa tgcttcatca
      421 ttgtgaggat ccatatatct caaataataa gcagaagaac ctgcaaaacc aggcatggta
      481 cacacttcca gtggataacc ttcttttgtt ttccagtttt tggctcttgc cagtggcggc
      541 tcaccatttt cagtaggaag atatttatca atttcgggaa gaaccaatgg aagttctttt
      601 tcgtccagca cgtaaggcat gccatcttta taataaaccg ggaaaggttc gccccaatat
      661 cgttgacgac tgaaaatagc atctcggatg cgataattta ctttgacgcg acctattcct
      721 ttttttgtaa tatatttttt tattttttca atcgcttctg gtacagaaag tccgttcaaa
      781 aattcagaat ttatcataat tccttcttta gcatcgaagc tttcgttgct gacatcgcaa
      841 ccttctatca gaggaacaat gggcaaatta aaatgttttg caaaagcata gtcacggctg
      901 tcatgtgcag gaacagccat aatagcgccg gtgccataac cagcaagaac ataatcagaa
      961 atccagatag gaatttcttt tccagtgata ggatgaatgg cataagagcc agtaaagaca
     1021 ccagttactt gtctatcggc aattcgttct cgctcggttc ttctttttgt tgcctctaaa
     1081 tattcttcta cttcttttct ttgttcaggt gtagtgcaga ttttaaccag ctcgctttca
     1141 ggagcaagca ccataaaagt tacaccaaaa attgtatcag gtcgagtggt aaatacatcg
     1201 aaacttacat tttttccttt aatggtaaaa cgaagttcgg ctccttcgct tcttccaatc
     1261 cagtttcgtt gagtttcttt caatgattca gaccaatcaa tttgttccaa accatcgagc
     1321 aaacgttgag catacgctga aacgcgtaaa caccattgcc gcatcacgcg ttgttccact
     1381 ggataaccac cgcgaatgga aacaccttcg cttacttcat cattggctaa aacagttcct
     1441 aacgctggac accagtttac tttcgtatcg gcaaggtaag caatgcgata attcaacaga
     1501 atttcctgtt gagctttttc actcattgct ttccattcat cagcagtaaa attcaattct
     1561 attgtaccaa cagcagttag attttcagta ccatattttt caaaatgttc aatcaacaaa
     1621 gtgatgggtt gggctttttg taatttttcg tcgtaataat gtttaaacat ttgaataaaa
     1681 gcccattgtg tccatttgta ataatctgga tcacaagtac gaatttcgcg actccaatcg
     1741 tagcaaaaac cgattttatt gagttgttcc cgataacgtt caatattttt cttcgttgtt
     1801 atagcaggat gctgtcccgt ctgaatggca tactgttctg ctggtaaacc aaaagcatca
     1861 aatcccatgg gatgaagtac attaaatccc ttcatccttt tataacgact ataaatatca
     1921 gaagcaatat aaccgagtgg atgtcctaca tgaagacctg ctcccgaagg ataagggaac
     1981 atatccaata cataatattt gggtttagtt aagtctattt cggtcttgta aatgttattt
     2041 tcgttccagt aattttgcca ctttacctcg atttccttaa aattatattc cataataaat
     2101 tgaaagttat taatttaagt tttttcctcc taaattttta catttacatt agtataaatt
     2161 tgcaaagtta attaatttca atcaataaat gaaggaggat tcttttagta gaattaaata
     2221 acgtaaagaa gagataaata aaaaaaatga ttatttttgt gagataaatg tttataatgc
     2281 cattgaagtg accaataaca aacc
//
LOCUS       DAOTKS010000266         2303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_37703_length_2303_cov_3.487989, whole genome shotgun sequence.
ACCESSION   DAOTKS010000266 DAOTKS010000000
VERSION     DAOTKS010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37703_length_2303_cov_3.487989"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2303
                     /gene="sprA"
                     /locus_tag="P5236_05560"
     CDS             <1..>2303
                     /gene="sprA"
                     /locus_tag="P5236_05560"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HRU63865.1"
                     /translation="MDPFIVDKDGTQKGGDLYFNLGDISEDILKDGKKFFEQGLPLDA
                     DITKVDTTVWGKIPKTQSTVKAFDNSPGARAKQDVGLDGLPTNEEQQFPTYKNYVEKI
                     NQKIDGETRQKWQSDPFSPLNDPAGDNYHFYRGSDYDAQEKDILSRYKYYNGTEGNSP
                     EAENTQENYSTSATSLPDGEDLNGDNTMNEYEKYYQYHVQIKREAMEVGRNYITDKIV
                     SNVKLENGKVEPVTWYQFKIPIREYDEKIGNIRNFKSIRFIRIFLTNFEQETHLRFAT
                     LDLVRGEWRSYTKSLFPAGSTPISEGKLDVHSVNIEEDADKTPVNYILPPGITRETDP
                     GQPQLLQLNEQSMALRIKDLAPNDARAVYKNTSYDMRQYKRLQMFVHAEKLVDDPSNL
                     QDYQLTCFIRLGSDMVNNYYEYEVPLRVTPHGKYLNEKNEDREIVWPLENMFDFPFST
                     LIEAKLKRDKYLQTSGGNATTLTPYEVYDPDNPKNKIRIVGNPSISDIENIMIGIRNV
                     SGEIKSGEIWVNELRMSKFNESDGWAAMGNLAVGLSDIGSLNFSGRIETAGYGSLESN
                     VMNRRLEDLRQMNFSTALEVGRFLPEKAKLQIPLYFSYTNETVSPKYNPLNQDVELKD
                     ALENLTSKTERDSLLNLSQEVNTSKSFNISNARINIRSKKPQFYDPANWRFTYAYIES
                     DKYTPEIEQDMNKSQRAAIDYSFNFNPQPWEPFKNIKSLNKPAFKIISDFNIYYLPSS
                     INFNTNLNRQYSQTKLRSLETSSVDIS"
BASE COUNT          819 a          373 c          412 g          699 t
ORIGIN      
        1 gatggatcct tttattgtgg ataaagatgg aacacaaaaa ggcggtgatt tgtattttaa
       61 tttaggagat ataagtgaag atattttaaa agatggcaag aaattttttg aacagggctt
      121 gcctcttgat gccgatatta caaaagtgga tactacggta tggggaaaaa ttccaaagac
      181 acaatctact gtcaaggctt ttgataattc tcctggagcc cgagccaaac aagatgtcgg
      241 tttagacgga cttcctacaa acgaagaaca acaatttcct acttataaaa attatgtgga
      301 gaaaattaat caaaaaattg atggtgaaac ccgccaaaaa tggcaaagtg atcctttttc
      361 tcctttaaat gatcctgcgg gggataacta ccatttttat agaggttcag attacgatgc
      421 tcaggaaaaa gatattttat ctcgttacaa atattataat ggaacggaag ggaattcgcc
      481 cgaagctgaa aatacacaag aaaattatag tacttctgct acttccttac ctgatggaga
      541 ggacttaaat ggtgataata caatgaatga atatgaaaaa tattatcaat atcatgttca
      601 aataaaaaga gaagcaatgg aagttggtag aaattatatt actgacaaga ttgtttcaaa
      661 tgtgaaattg gaaaacggta aagttgagcc agttacctgg tatcaattta aaattcctat
      721 tagggaatat gacgaaaaaa ttggtaatat ccgtaatttt aaatctattc gttttataag
      781 aatatttttg accaattttg aacaagaaac acatttacgt tttgctacac tcgatttagt
      841 aagaggtgaa tggcgaagtt atactaaatc actttttcca gcaggctcaa ctccaatttc
      901 agaagggaaa ctcgatgttc attctgtcaa tatagaagaa gatgcagata aaactcccgt
      961 caattacatt ttacctcctg gcattacgcg tgaaactgat ccgggtcaac cacaattatt
     1021 gcaactcaat gaacaatcaa tggctcttcg gataaaagat ttagctccca atgatgcaag
     1081 agcagtttat aagaacactt cgtatgatat gagacaatat aagcgtttac aaatgtttgt
     1141 acatgctgaa aaattagtgg atgatccgag caatcttcaa gattaccagc ttacatgttt
     1201 cattcggtta ggttctgata tggtgaacaa ttattacgaa tatgaagtcc ctcttcgcgt
     1261 gacgccacat ggcaaatatt tgaatgaaaa aaatgaagat cgggaaatcg tttggcctct
     1321 tgaaaacatg tttgattttc ctttttctac tttaattgaa gcaaaactga aacgcgataa
     1381 atatctgcaa acttctggag gaaacgccac aactttaaca ccctatgaag tatatgatcc
     1441 agataacccc aaaaataaga ttagaattgt tgggaatcct tctatttcag atattgaaaa
     1501 tatcatgatc ggtattcgaa atgtaagtgg cgaaattaag tcgggagaaa tttgggtcaa
     1561 tgagcttcgc atgtcaaaat tcaatgaatc agatggttgg gctgcaatgg gaaatttagc
     1621 ggtaggactt tctgatattg gttcacttaa tttttcaggt cgtatagaaa cagcaggtta
     1681 tggaagcctt gaaagtaatg tgatgaatcg ccgattggaa gacttgcgac agatgaattt
     1741 ttcaactgcc ttagaagtag gaaggttttt acctgaaaaa gcaaagctgc aaattcccct
     1801 ctatttttct tatacgaatg aaactgtttc gcctaaatat aatccactga atcaagatgt
     1861 cgaattaaaa gatgcactgg aaaatttaac ttcaaaaaca gaacgagatt ccttattaaa
     1921 tctttcacaa gaagtaaata cttctaaaag tttcaatatc tctaatgcaa gaattaacat
     1981 aagaagtaaa aaaccccaat tttatgaccc agctaattgg cgatttactt atgcttatat
     2041 tgaatccgat aaatatactc ctgaaattga gcaggatatg aataaaagtc aacgagctgc
     2101 cattgactat agttttaatt ttaatccaca accgtgggaa ccattcaaaa atatcaaatc
     2161 attaaataaa ccagctttta aaatcattag tgatttcaac atttattatt taccttcttc
     2221 tattaatttt aataccaatt taaatcggca gtattctcaa actaaattgc gatcgcttga
     2281 aacttcatct gtcgatattt ctg
//
LOCUS       DAOTKS010000267         2301 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_37741_length_2301_cov_2.826803, whole genome shotgun sequence.
ACCESSION   DAOTKS010000267 DAOTKS010000000
VERSION     DAOTKS010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37741_length_2301_cov_2.826803"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..>2301
                     /locus_tag="P5236_05565"
     CDS             22..>2301
                     /locus_tag="P5236_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63866.1"
                     /translation="MKLSAGTWVIGVNQVDRFLKAIEKGGGEEPPAIPLKDDGTINTN
                     YYGVWEIFPGITCTSEDIVPFYMEGQGSDHHWRGIKKINYANKGYKQKSGTVISFVQP
                     SLNASDYPEIPTGYTSLSNYWDRNDLSWLEIIDFSGNDFENIEVDGHNIMPLTEISLA
                     NNPHLKSLIIKDCPSLTNVNITGCNLTFSFISNLRNIINLSANAEFLYEYQGEITSYY
                     AAVDLTELLEKNGEGSRIKSWSIQPIKNEGNIYTFDSSHVGEVVRVSLENSDYPGIII
                     SYDINLTSAIPSTISINATYCKVKIQNLTTGNNNFSIGDEALITVYPYSCSVLESFTI
                     DGEEVTLDSKYQYYFIVNKTFHQITSIFKKDDMPTGQTSNYLRNGSFEYGLNLDWEYN
                     IGEGTSAEFTLERGSNVIDGNVALRVDVNSLKTPNSVSAKTHVAVGCDSLYLLHFWAK
                     GPEESKLYVQIDGAEQNGIQYELHEGATAYFYPIKIDKTKANEGLTITFYFQNDFTKV
                     KVADPCSVTTYAGATYYLDDLELVDQFNNLTYDVYNTYLWNYNHRPNNEGKMWVAGDN
                     SVSYDLPDGRKMWFFNDSFYGVLHPESNRLVDVGRFVRNAVVVQNIDGTLTTYPVTNQ
                     GGQWTYFRVPDDAVIYNDDGSVKNIFWVGDALMEDNQIKVYLIEVYGEGRSYLGKFSY
                     PGLEFLGVEKQENFCRKYETFFVEDNKIYLYKTEDVGTWGRYMHVARADLGDLSGKKG
                     TWEFWNGTEWSKDSTQTARI"
BASE COUNT          792 a          313 c          452 g          744 t
ORIGIN      
        1 tgtttgttag catttaatat tttgaaatta tctgccggca catgggttat aggtgtaaat
       61 caagtagatc gttttttaaa agccattgaa aaaggaggtg gagaagaacc accggctata
      121 cccttaaaag atgatggaac aattaataca aattattacg gagtgtggga aatttttcca
      181 ggtatcactt gtacttctga ggacattgta ccattttata tggaaggtca aggttccgat
      241 catcattgga gagggattaa aaaaatcaac tatgctaata aaggttataa acaaaaaagt
      301 ggtaccgtta ttagctttgt acaaccaagc ttgaacgcat cggattatcc tgaaattcct
      361 accggttata ccagcctttc taattattgg gatcgaaatg atctttcttg gttagaaatc
      421 attgattttt ctgggaatga ctttgagaac attgaagttg atgggcataa tattatgcca
      481 ttaacggaaa tatcattagc aaataatcct catttgaaaa gtttaataat aaaagattgc
      541 ccaagtttaa caaatgttaa tatcaccgga tgcaatttaa ctttttcgtt tatttccaac
      601 ttacgaaata taataaattt atcagctaat gcagaattct tatatgaata tcaaggagaa
      661 attacttctt attatgccgc tgttgattta actgaattac ttgaaaaaaa tggagaagga
      721 agtcgaataa aatcatggtc aatccagcca ataaaaaatg aaggaaatat ttataccttt
      781 gactcaagtc acgtgggaga ggtcgtacgt gtttctctgg aaaattcaga ttatcccggt
      841 ataattatat cctacgatat aaacctgaca agtgctattc catccactat ctcaataaat
      901 gctacttatt gtaaagtaaa aatacaaaat ctgacaacag ggaataataa tttttctatt
      961 ggagatgaag cattaattac agtatacccc tattcttgct ctgttctcga atcatttaca
     1021 attgatggag aggaagttac attggattct aaatatcaat attattttat tgtaaataaa
     1081 acttttcacc agattacttc aatctttaaa aaagatgata tgccaactgg acaaacgtca
     1141 aattatttgc gaaatgggag ttttgagtat ggtctaaatt tagattggga atataatatt
     1201 ggagagggaa cttcggcaga gtttacgttg gagcgaggta gtaatgtaat agatggcaat
     1261 gttgctttaa gggttgatgt taattcttta aaaactccta attcagtttc tgctaaaaca
     1321 catgttgcag ttggttgtga ttctctttat ctattacatt tttgggcaaa aggtccagaa
     1381 gaaagtaaat tatatgtcca gatagatggg gcagaacaaa atggaataca atatgaactt
     1441 cacgaaggag ccactgcata tttttatcct attaaaattg ataaaaccaa agccaatgaa
     1501 ggattgacaa tcactttcta ttttcaaaat gattttacaa aagtaaaggt tgcggatcca
     1561 tgtagtgtga caacttatgc aggagcaact tattatcttg atgaccttga gttagttgac
     1621 caatttaata atttgactta tgatgtttac aatacttatc tctggaatta taatcataga
     1681 ccaaataatg aaggaaaaat gtgggtagca ggagataata gtgtttcata tgatttacca
     1741 gatggaagga aaatgtggtt tttcaatgat tccttttatg gggtgctaca tcctgaaagt
     1801 aatcgacttg ttgacgtagg tcggtttgtt cgaaatgcgg tagttgttca aaacattgat
     1861 ggaacattaa caacttatcc tgttactaat cagggaggtc aatggacata ttttcgggtt
     1921 cccgatgatg cagtaattta taatgatgat ggaagtgtca aaaatatatt ttgggttggt
     1981 gatgctttaa tggaagataa tcaaattaaa gtgtatttaa ttgaagttta tggtgaaggg
     2041 cgatcgtatt taggaaaatt ctcttatcct ggccttgaat ttttaggtgt cgaaaaacaa
     2101 gaaaattttt gtagaaaata tgagactttt tttgtggaag ataacaaaat ttacttatac
     2161 aaaactgaag atgtaggtac atggggacgt tatatgcacg ttgccagagc tgatttggga
     2221 gacttgagcg gaaaaaaagg aacttgggaa ttttggaatg gaaccgaatg gtcaaaagat
     2281 agtactcaga cagccagaat t
//
LOCUS       DAOTKS010000268         2300 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_37760_length_2300_cov_4.083296, whole genome shotgun sequence.
ACCESSION   DAOTKS010000268 DAOTKS010000000
VERSION     DAOTKS010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37760_length_2300_cov_4.083296"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /gene="rsgA"
                     /locus_tag="P5236_05570"
     CDS             complement(<1..578)
                     /gene="rsgA"
                     /locus_tag="P5236_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRU63867.1"
                     /translation="MKGLVVKNTGSWYEVKTNDGKRINCKIKGNFRLKDIRSTNPIAV
                     GDWVEFEINKEGKGIIYNICERKNYIIRRSSNFSKEVSILAANLDAAALVVTVNYPET
                     STVFIDRFLAVAEAYSVPACLIFNKIDLYSEEEKEYLDAITYLYESIGYPVFQVSALY
                     AINLAPLTDFLKDKITLFAGNSGVGKSALINAL"
     gene            complement(741..1304)
                     /gene="frr"
                     /locus_tag="P5236_05575"
     CDS             complement(741..1304)
                     /gene="frr"
                     /locus_tag="P5236_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRU63868.1"
                     /translation="MTEEIKPILDEAKKKMEETIRFLESSLARIRAGKANPHILDVVK
                     VEYYGNHVPLSNVASITTPDAKTILIQPWEKTMIKAIEKAILNANIGFTPTDNGEVIR
                     LTLPPMTEERRKELVKQVRIEAEEARITIRNARRDVIEQIKKKVKEGLSEDIEAVGTE
                     EAQKLHDNYVKKIDDLLAAKEKEIMTV"
     gene            complement(1410..2126)
                     /gene="pyrH"
                     /locus_tag="P5236_05580"
     CDS             complement(1410..2126)
                     /gene="pyrH"
                     /locus_tag="P5236_05580"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRU63869.1"
                     /translation="MLLYKRILLKLSGESLMGTKQYGLDESRLQEYATQIAEISSMGV
                     EVGIVIGGGNIFRGINGESKGFDRVLGDQMGMLATVINSLALCSALQVIGAKSRVLTA
                     IRMEPIGEFYSKQKAVSCLQQGEIVIISGGTGNPFFTTDTASSLRAIEIGADVMLKGT
                     RVDGVYTADPEKDANAVKFDEITYDEIYRRNLKVMDLTATTLCKENNMPIYVFNMDKA
                     GNLKKVVLGEKIGTLVKNNF"
BASE COUNT          749 a          441 c          310 g          800 t
ORIGIN      
        1 agtgcattaa taagtgccga tttccctacc cctgaattac cagcaaaaag agtaattttg
       61 tcctttaaga aatctgttaa gggagctaaa ttgatggcgt ataaagccga aacttgaaaa
      121 acaggatagc caatgctttc atacaaataa gtaatagcat caagatattc tttttcttcc
      181 tcagaatata aatcaatttt gttaaatatc aggcaagctg gaacactgta agcttcagct
      241 actgctaaaa aacgatcaat aaaaacggtt gaagtttcag gataatttac tgtcaccact
      301 aaagctgctg catccagatt agcagctaaa atgctaactt cttttgaaaa attggacgaa
      361 cgacgaataa tataattttt cctttcacag atattataaa ttattccttt accttctttg
      421 tttatttcga attctaccca atcacccact gcaataggat tggtgctacg aatatctttt
      481 aaacgaaaat tacctttaat tttacagtta attcgtttac catcgttagt tttcacctca
      541 taccaactcc ctgtattttt tactacaaga cctttcatga ataattcaat tgaaaattta
      601 ttaggtaggc atttagcaat aaatgaaaaa attaaaatag aaaggaattt tttgttttgg
      661 aaaaataaaa aatcaattga taaaattctt ttaaggaagg aggaattcta tcaattgaac
      721 cttaatatcc gcaccaatat ctaaactgtc ataatttctt tttctttggc agcaagcaaa
      781 tcatctattt ttttaacata gttatcatga agcttttggg cttcttctgt accaacagct
      841 tcaatatctt ctgataaacc ttcttttact tttttcttaa tttgctcaat cacatctcga
      901 cgagcattac gaatagttat tctggcttct tctgcttcta tgcgaacttg ttttactaat
      961 tctttgcgac gctcttccgt cattggcggt aaagttaaac gaataacttc tccgttatcg
     1021 gttggagtaa aacctatatt ggcatttaaa attgcttttt cgatagcttt tatcattgtt
     1081 ttttcccacg gttgaatcaa aatagtttta gcatcaggtg tagtaataga agctacatta
     1141 cttaatggta catgattccc ataatattcc acttttacca catctaaaat gtgaggatta
     1201 gctttccctg cacgaatacg cgctaatgaa ctttctaaaa aacgaatggt ttcttccatt
     1261 ttcttttttg cctcatccag aattggttta atctcttctg tcatcataat ggtaattttt
     1321 atcacttaat tctgcataac agaaattagt gtggttaatt aagtttatta tttataaatg
     1381 aaataattaa aaatatcctc caagttttct taaaaattat tttttactaa agtgcctatt
     1441 ttttcaccca aaactacttt tttcaaattg ccagctttat ccatattaaa gacataaata
     1501 ggcatgttat tttccttaca aagggttgtt gctgtcaaat ccattacttt caaatttcgg
     1561 cgataaattt catcgtaagt aatttcgtca aattttacag cattggcatc tttttctgga
     1621 tcggcagtat atacaccatc tacacgtgtt ccttttaaca taacatcagc tcctatctct
     1681 attgctcgta atgaggaagc tgtatcggtg gtaaaaaatg gattccctgt acctcctgaa
     1741 ataattacaa tttctccctg ttgaaggcaa gaaacagctt tttgtttgct ataaaattct
     1801 cctatcggtt ccatccttat ggctgtcagt acccgtgatt ttgctccaat cacttgcaaa
     1861 gccgaacaca gcgccaagct attaatgacc gttgccaaca ttcccatctg gtcacctaaa
     1921 acacgatcaa aaccttttga ttcaccattt attccacgaa aaatattccc tccgccaatt
     1981 actattccta cttctacccc cattgacgaa atttcagcga tttgagttgc atattcctgc
     2041 aaccgacttt catcaagacc gtattgcttt gttcccatca aagattcacc gctcagtttc
     2101 aacaaaattc gtttataaag cagcatataa aatatttttt attttcgata gcttcttctt
     2161 ataaatctac aaaaaagaaa agattatcag acaaaaataa ataaatagaa tggtatttac
     2221 aaacaactga gaaaaagaaa aaataaagta gagtcaacca gcaagtgcga aattataaaa
     2281 aatgtttgaa aaactcattt
//
LOCUS       DAOTKS010000269         2295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_37859_length_2295_cov_4.651786, whole genome shotgun sequence.
ACCESSION   DAOTKS010000269 DAOTKS010000000
VERSION     DAOTKS010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37859_length_2295_cov_4.651786"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..1164
                     /gene="dnaN"
                     /locus_tag="P5236_05585"
     CDS             31..1164
                     /gene="dnaN"
                     /locus_tag="P5236_05585"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRU63870.1"
                     /translation="MEFVVSSTELLNRLQTISRVINPKNALQILDNFLFELKGNTLTV
                     VATDIETTLITSLEVINAKGSGRVAITSRLLLDTLREFSEQPLTFSINDTNLSLTILS
                     ANGTYNFIGQNADEYPRIPEIDEENSNLVVPGVNLFSGISKTLFASVDDDFRPVMSGI
                     YFDIVPEGLTIVATDAHKLVRFKTIYTSASLKEEAMSFILPKKPANLLKNILPKESGD
                     VLIKFDEKRVLFQLSNYTLIGLQIEGRYPNYNAVIPTSNPYKVIIDRVNFLNAIKRVS
                     IFSNQASNLIKLVFENQQVHISAQDIDFSISAEEVIPCQFEGETFTIGFKATFLIEIL
                     SSLDSSEVIIELSDPTHGALFFPFEKKENEEVVMLLMPMLLND"
     gene            1275..2042
                     /locus_tag="P5236_05590"
     CDS             1275..2042
                     /locus_tag="P5236_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HRU63871.1"
                     /translation="MQLQLKKPLAFLDLETTGTNIVTDRIVEISYYKVYPNGREETKT
                     IRINPEMHIPEKATAIHGITDEDVKNCPTFKEVAKEIARDLEGCDLAGYNSNKFDIPL
                     LAEEFIRADVDIDLMKHKFIDVQVIFHKMEQRTLSAAYKFYCDKDLMNAHGAEADAKA
                     TYEILKAQLDRYSELKNDVDFLSDFTTQTKNVDFAGRIIYNENGEEIINFGKYKGQKV
                     TDVLKKDIGYYGWIMNGDFSLHTKKILTAIKLREFNK"
     gene            2156..>2295
                     /locus_tag="P5236_05595"
     CDS             2156..>2295
                     /locus_tag="P5236_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HRU63872.1"
                     /translation="MNKIVSKEYLSPNVVKFEVEAPLIAKARKAGHFVMVRVGEKGER
                     IP"
BASE COUNT          787 a          320 c          391 g          797 t
ORIGIN      
        1 aatattcata aattaaccaa gaggaatgat atggaatttg ttgtttctag caccgaactt
       61 ttgaatcgtt tacagacaat cagtcgagta attaatccca aaaatgcttt gcagattttg
      121 gataattttt tatttgagct caaaggaaat actttaacgg ttgttgcaac tgatattgaa
      181 accactttaa ttacctcttt ggaagtaatt aatgccaagg gaagtggaag agttgctata
      241 acttcccggt tattattaga cacattacgt gaattttcag aacagcctct tactttttct
      301 attaatgata caaatttatc acttaccatt ttgtcagcca atggaactta taattttatt
      361 ggacaaaatg ctgatgaata tcctcgtatt cctgaaattg atgaagaaaa ttcaaattta
      421 gttgttccag gagtcaattt attttctgga atttctaaaa ctttatttgc atcagtagac
      481 gatgattttc gtccagtgat gagcggtatt tattttgata tcgttccaga aggtttgaca
      541 atagtggcta ctgatgcaca taaattggtg cgtttcaaaa caatttatac ttcagcatcc
      601 ttaaaagagg aagcaatgag cttcattctt ccaaaaaaac cagctaattt attgaaaaat
      661 atcttgccta aagaaagcgg agatgtatta attaaatttg acgaaaaacg tgttttgttc
      721 cagttaagta attatacact cattggttta cagatagaag gacgatatcc caattataat
      781 gccgttattc ctacttcaaa tccttataaa gtcattattg atagggtaaa ttttttgaat
      841 gctattaaac gtgtttctat tttttcaaac caggcaagta atttgattaa attggttttc
      901 gaaaatcaac aagttcatat ttctgctcaa gatattgatt tttctatttc tgctgaagaa
      961 gtaattccct gtcaatttga aggtgaaaca tttactatag gttttaaagc aactttttta
     1021 atagagattt tatcctcact tgactcatcg gaggtaatta tcgagttgtc tgatccaact
     1081 catggtgctc ttttctttcc tttcgaaaag aaagaaaatg aagaagtggt tatgttgcta
     1141 atgcctatgt tgttaaatga ttaatcatga aatcttatat ggtataataa ggtctaaaga
     1201 ggttgcttta gaccttgttt ttattatttt tagaagaaga agttattata taatttaaac
     1261 ccccatcctt tattatgcag cttcaattga aaaaaccatt agcgttttta gatctggaaa
     1321 caacagggac caatatcgtt acagatagaa ttgttgaaat atcttactat aaagtttatc
     1381 caaatggcag agaagaaact aaaactattc gaattaatcc tgaaatgcat atccctgaaa
     1441 aagctaccgc cattcatgga ataacagatg aagatgtaaa aaactgtccc actttcaaag
     1501 aagttgcaaa agaaattgca cgtgatttag aaggttgtga tttagctggt tataattcaa
     1561 ataaatttga catcccgctt ttggctgagg aatttattcg tgctgatgtc gacatcgact
     1621 taatgaaaca taaatttatt gatgttcaag ttattttcca taaaatggaa caacgcactt
     1681 tatcagcagc atataaattt tattgtgaca aagatttaat gaatgcacat ggcgctgaag
     1741 ctgatgctaa ggctacttat gaaattttaa aagctcaact ggatcggtat tcggaattga
     1801 aaaacgatgt ggatttcctc tccgatttta ctacacaaac taaaaatgtt gattttgctg
     1861 ggcgaattat ttataatgaa aatggtgaag agattatcaa ttttggaaaa tataaaggac
     1921 aaaaagttac cgacgttcta aaaaaagata taggatatta tggttggatt atgaatggtg
     1981 atttttcatt acatactaaa aaaatactga cagctattaa attacgcgaa tttaataaat
     2041 gattgtttga tgattggtat tgtagattca aaataatttt gatttttttt gttctttaaa
     2101 tttactgaat atgaatttta attttcaatc ctgaaaaatt aaaaataaag ataaaatgaa
     2161 taagattgtt agcaaagaat atctttcccc caatgtagtt aagtttgaag ttgaagctcc
     2221 tttgattgct aaggcaagaa aagcgggtca ttttgtgatg gtacgggttg gtgaaaaagg
     2281 cgaacgaatt ccttt
//
LOCUS       DAOTKS010000270         2289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_37988_length_2289_cov_4.183975, whole genome shotgun sequence.
ACCESSION   DAOTKS010000270 DAOTKS010000000
VERSION     DAOTKS010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37988_length_2289_cov_4.183975"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2289)
                     /locus_tag="P5236_05600"
     CDS             complement(<1..>2289)
                     /locus_tag="P5236_05600"
                     /inference="COORDINATES: protein motif:HMM:NF012433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HRU63873.1"
                     /translation="RSIYRPGQTVYFKGIAYFSDAKRQEVDKFSKYEVSLFDANHQKI
                     NSKTFTTNEFGSFTGTFILPENGLNGTFTLECSGFSASFQVEEYKRPTFEVKLEKPLS
                     EISFGENVLLKGNAVAYAGYPVVQANVTYRIVRRNHYLWWYPEVKEVANGKTTTDENG
                     NFEINFIPLKDQKKKEQYYTYTIYVSITDTKGETQQAQQTISVGDKSLFILAEVPPKW
                     DKAEKLCINVSLETLNGNKIDGKLNYAIYQLSASVDFEEEDADIKTNSDRVLTKIADG
                     IFETSNKQLEFDFRHYKSGRYRIIFTAFDTKGKEVKLEKDFVLYSLTDKRPPVKTYTW
                     LISPKTEVAPNENAKIYFGTSTTDTEILYELMRGNKTLESEWIKFNNEIKTFEIPFAT
                     IKEGGVTVMFTFVKNEKIFRKQISLKQKKSENKLTPFFSVFRDKLSPNETAEWTVTIP
                     ELKNSGKTAELLTTMYDASLDAIYPHNWYFYPVYQETFVPSPIWQCRVEEQERDFVSV
                     KTDFLPVKNFELDQINWFGLSLLSNVPYLRPMRMGSLPVQKQEVLLNQSDQIAGAKPF
                     GIINEELETTDLAKEPVQIRSEFNETAFFYPQLHTDSLGNVKFSFTVPESLTRWDIKM
                     LAHTPDLYFGQADTQVVVQKELMVQMNLPRFVHRGDTLMLTANVVNLTHENQTVKVTF
                     EMLQPETEQNISLPDVASKTIVLTPQQTRVVEWKVSQFTGFDLVICKVVAQSENFSDG
                     EQKYLPILPDRILITESLPVIVR"
BASE COUNT          695 a          431 c          367 g          796 t
ORIGIN      
        1 acgaacgata acaggcaaac tttccgttat caaaattctg tcaggcaaaa tgggtaaata
       61 tttttgttcg ccgtcactga aattttcact ttgagccacg actttacaaa ttaccaaatc
      121 gaaacctgta aattgtgata ctttccattc taccactctg gtttgttgag gtgtaagcac
      181 tattgttttt gatgctacat caggcagaga aatattttgt tctgtttcgg gttgcaacat
      241 ttcaaaagtt acttttaccg tttgattttc atgggtcaaa tttaccacat tagcagtcaa
      301 catcaacgta tcacctcgat gtacaaaccg tggcaaattc atttgaacca tcaattcctt
      361 ttgaacaaca acttgcgtat cagcttgtcc gaagtataaa tcgggcgtat gagccagcat
      421 tttaatgtcc catcgggtta aactttccgg taccgtaaag gaaaatttta catttcccaa
      481 cgaatctgta tgcaattggg gatagaaaaa ggctgtttca ttaaattcac tgcgaatttg
      541 aacaggttct ttggctaaat cagttgtttc aagttcttca tttataatac caaatggttt
      601 tgcaccagca atttgatcac tttgattcaa caatacttct tgtttctgaa ctggtaaact
      661 tcccatcctc atgggtctta ggtaaggaac atttgacaat aaactcaaac caaaccaatt
      721 gatttgatcc aattcaaaat tttttacagg caaaaaatca gttttaacac ttacaaaatc
      781 cctctcctgt tcttcaaccc tgcattgcca aatgggtgaa ggaacaaaag tttcttgata
      841 aacgggataa aaataccaat tatgcggata aatagcatcc agcgaagcat catacatggt
      901 tgtaagcaat tcagcagttt ttccactgtt ttttagttct ggtattgtta ctgtccattc
      961 tgcagtttcg tttggtgaca atttatccct gaaaacgcta aagaaaggcg ttaatttatt
     1021 ttcggatttt ttttgtttaa gagaaatttg tttcctgaaa attttctcat tcttcacgaa
     1081 agtaaacata actgttactc caccttcttt tatcgtcgca aaaggtattt caaaagtttt
     1141 aatttcattg ttaaatttta tccattcgct ttccagcgtt ttattaccac gcatcaactc
     1201 ataaagtatt tctgtatcgg tagtagaagt cccaaaataa atttttgcat tttcattagg
     1261 tgctacttct gttttgggtg atataagcca tgtataagtt tttacgggag gacgtttatc
     1321 cgttaaactg taaagaacaa aatctttttc cagttttact tcttttcctt ttgtatcgaa
     1381 agcagtaaaa ataatccgat aacgacctga tttataatga cgaaaatcaa attccagttg
     1441 tttgttactg gtttcaaaaa tgccgtcagc tattttagtt aaaacccgat cagaatttgt
     1501 tttaatgtca gcatcttctt cttcaaaatc taccgaagca gacaattgat aaatagcata
     1561 attcaattta ccatcgattt tattgccatt gagcgtttcc agactaacat ttatacacaa
     1621 tttttctgct ttatcccatt ttgggggaac ttctgctaaa atgaaaagtg atttgtcacc
     1681 cactgaaatg gtttgctgag cttgttgcgt ttcacctttt gtatcggtga tagaaacata
     1741 aatggtatat gtataatatt gctccttttt cttctgatct tttaagggaa taaaattgat
     1801 ttcaaaattt ccattttcat ccgtagtagt ttttccattg gctacttcct tcacttcagg
     1861 ataccaccat aaatagtgat ttcttcgtac aattcggtaa gttacgttgg cttgaactac
     1921 aggataacct gcataagcaa cggcattccc tttcagcaaa acgttttctc caaaagaaat
     1981 ttccgaaaga ggtttttcca gctttacttc aaaagtagga cgcttatatt cttctacttg
     2041 aaaagaggca gaaaatccac tgcactcgag cgtaaaagtt ccgtttaatc cattttccgg
     2101 taaaataaat gttcctgtaa aagaaccaaa ctcgttggta gtgaatgttt tagaatttat
     2161 tttctgatga ttggcatcaa aaagcgatac ttcatatttt gaaaatttat ccacttcctg
     2221 acgtttagca tctgaaaaat aagcaatgcc tttaaagtaa accgtttgtc caggacgata
     2281 aatggaacg
//
LOCUS       DAOTKS010000271         2281 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_38154_length_2281_cov_4.453279, whole genome shotgun sequence.
ACCESSION   DAOTKS010000271 DAOTKS010000000
VERSION     DAOTKS010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38154_length_2281_cov_4.453279"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1351
                     /locus_tag="P5236_05605"
     CDS             <1..1351
                     /locus_tag="P5236_05605"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HRU63874.1"
                     /translation="HSIPISATFNVLKYINISPTFNYTERWYLQSIHKDWDTENQKVK
                     IDTISEFHRVYDFNMGISASTKIYGFFIPIRSIFGDKVDRIRHVFTPSIGLGYTPNFG
                     DSRWGYYDSYDKITKDPTDPDVLYHEQVSYSRYEGSLYGYPGTGKSGSINYSFANNLE
                     MKVRNDKDTTGTVPYKVISLIDNFSVSGSYNMVADSMNWSNFNVNLRLKFGQNRSLNL
                     SGSFDPYMYGLNKSGQPVRINELRWNHGKFPRFLGTNTSYSFTFNNDTFKKLFGKSKT
                     ASSSSQSNPLDAENEQSLPADQSSEQSSSPLISSTSHYSSSAETDKDGYQKITIPWNL
                     TVNYSIRYANTNVFDYKKMEYKMGFTHNLSLSGSISLTSKWNISSSTSYDFVAKQFTY
                     TNVSVSRDLHCWNISANFVPFGPFQSYYFRIGVNSSMLRDLKYEKRSGYGSTPIDWY"
     gene            1459..2031
                     /locus_tag="P5236_05610"
     CDS             1459..2031
                     /locus_tag="P5236_05610"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRU63875.1"
                     /translation="MKIDVNKVVSAKYELFVEGENEGELKLMERATEQEPLSFIYGVG
                     MILPKFEEYLYGLEEGDDFEFTIDSEDGYGHYDKEDIIDLDRSIFETDGKLDNNVIFE
                     GNVVTLMDNEGNRLPAKIVTITENYVTVDLNHPLADEKLHFKGTVLEVRDATEEELSS
                     LSGCGCDCEADCDCESNHANNSDCSDCECN"
     gene            2126..>2281
                     /locus_tag="P5236_05615"
     CDS             2126..>2281
                     /locus_tag="P5236_05615"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009236270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRU63876.1"
                     /translation="MANTIGKLFTFTSFGESHGPAIGGVIDGCPAGLKIDENFIQNEL
                     NRRRPGQS"
BASE COUNT          772 a          307 c          418 g          784 t
ORIGIN      
        1 acattctatt ccgattagtg ctacctttaa tgttttaaaa tacatcaata tttctccaac
       61 gtttaattat actgagagat ggtatttaca atcaatccac aaagactggg acactgaaaa
      121 tcaaaaagta aaaattgata caattagtga atttcatcgg gtatatgatt ttaatatggg
      181 aatttcagct tctacaaaaa tttatggttt ttttattcct attcgttcca tttttgggga
      241 taaagtggat agaattcgtc atgtgtttac tccaagtatc ggtctcggtt atacgcctaa
      301 tttcggagat tcacgatggg gttattatga cagctatgac aaaataacaa aagatccaac
      361 cgatcctgac gtgctttatc atgaacaagt atcctattct cgatacgaag gaagtttata
      421 tggttatcct ggaactggaa aatcgggtag tatcaattat tcatttgcaa acaatctgga
      481 aatgaaagtt cgcaacgata aagataccac tggaactgtt ccttataaag tgataagttt
      541 aatcgataat ttttcagtta gtggaagtta taatatggta gctgattcca tgaattggtc
      601 aaattttaat gtcaacttgc gtttaaaatt tggtcaaaat cgttccctaa atttaagtgg
      661 aagttttgac ccttatatgt atggtcttaa taaaagcgga caaccagtta gaattaatga
      721 attgcgttgg aatcatggga aatttcctcg ttttttgggg acgaatacaa gttatagttt
      781 tacttttaat aatgatactt tcaaaaagct ctttggaaaa agtaaaactg cttcgagttc
      841 aagtcaatca aatccattag atgctgagaa cgaacaatct ctgcctgcag atcaaagttc
      901 tgagcagagt tcaagtccat taatttcaag cacttcacac tactcttcaa gtgctgaaac
      961 cgataaagat ggttaccaaa aaattacaat tccctggaat cttacagtta attattctat
     1021 tcgttacgct aataccaatg tttttgatta taaaaaaatg gaatataaaa tgggctttac
     1081 tcataattta agtttatcag gtagcatatc cttaacttcg aagtggaata tcagttcaag
     1141 cacttcttat gattttgttg caaaacaatt tacttataca aatgtaagtg tttcaagaga
     1201 tttacattgt tggaatattt ctgctaattt tgttcctttc ggacctttcc agtcttatta
     1261 ctttagaatt ggcgtaaatt cgagcatgct tcgtgacctg aaatatgaaa aacgtagtgg
     1321 ttatggttca acacccattg attggtattg agttataact gaaaaataaa gagattttac
     1381 ttttaaaatg aacaatttat tttaatagtc gttataaaaa aataattaat cttgatttat
     1441 aaatttaaaa attagaaaat gaaaattgat gtaaacaaag tagtgtctgc aaaatatgaa
     1501 ttatttgtag aaggagaaaa tgaaggtgaa ttgaaattaa tggaaagagc cactgaacaa
     1561 gaacctttaa gttttattta tggtgtagga atgatcttgc ctaaatttga agaatatttg
     1621 tatggtttgg aagagggaga tgattttgaa tttactattg atagcgagga tggatatggt
     1681 cattatgata aggaagatat aatagacctt gatcgaagta tttttgaaac agatggcaaa
     1741 ttagataata acgtcatttt tgaaggaaat gtagttacct tgatggataa tgaaggtaat
     1801 cgtttacctg ctaaaattgt aacaatcact gaaaattatg tgacggttga tttgaatcat
     1861 ccactggcag atgagaaact tcatttcaaa ggaactgttt tggaagtgag agatgctacg
     1921 gaagaggaac tttcttcact ttctggatgc ggttgcgatt gtgaagcaga ttgtgattgc
     1981 gaaagcaatc atgctaataa tagcgattgt agtgattgtg aatgtaatta gttttatatc
     2041 aataaattac taaaagcata atttttgcct ttttaatgag aatttatttt tttttgtaaa
     2101 gcgagataaa aaagtttaat ttcttatggc aaatacaatt ggtaagttat ttacttttac
     2161 ctcttttggt gaatctcatg gacctgctat cggaggcgtt attgatggtt gtcctgctgg
     2221 gctcaaaata gatgaaaatt ttattcaaaa tgaattaaat cgacgtcgtc ccggtcaatc
     2281 t
//
LOCUS       DAOTKS010000272         2279 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_38201_length_2279_cov_4.687050, whole genome shotgun sequence.
ACCESSION   DAOTKS010000272 DAOTKS010000000
VERSION     DAOTKS010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38201_length_2279_cov_4.687050"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1907
                     /locus_tag="P5236_05620"
     CDS             <1..1907
                     /locus_tag="P5236_05620"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRU63877.1"
                     /translation="SVDGLVFTNHHCGFNAIQQHSTIEHNYLKNGFTAQKLEDEIPTP
                     GLTVTFLVRIEDVTNNILSQIPDSLTGINREDKISIISDSLSHAAIKDSSYTAKVLPF
                     FNENQFYLFVYEKFEDVRLAFAPPSSIGKFGGDTDNWMWPRHTGDFSVFRVYCSPDGK
                     PATYSTDNVPYSPKRFIPISNKGYKEDDFTMILGNPGSTSRYLSSFGIDYRMKVLNQA
                     RIDVRGIKQDIWRSFMNSNEAIQIAYANKYASSSNYWKNSIGMNQAIVKLKVMERKKA
                     EEAQFTEWLKTSPERQKKYNDVLTSLEEGYAKMYPYGKALIYMYESLINGVELPRIAE
                     NIQQWHNFNFSNDEILKKAEKLYKDYYPEVDQKVFIAMLKVYKKSVDADALPEIYEVI
                     QKKFKGEEEKYVQYLFDKSSFTTYEKLKKALLNNKIDFKNDPALVFSSVVKQTYNNIN
                     LKLSPINDQIKEVEKIYMAGLMEKAQEEGKQLYPDANFTMRMTYGKVAGYVPADAVTY
                     RYYTTTKGILEKENPSNEEFIVPSSLKKAIENKNFGNYADKNTGEMQVNFITNNDITG
                     GNSGSPIFNAKGELIGLAFDGNWESLSGDIIFEPDLQRTIGVDIRYMLFVMDKIGNAE
                     RLLKELNICN"
     gene            2047..>2279
                     /locus_tag="P5236_05625"
     CDS             2047..>2279
                     /locus_tag="P5236_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HRU63878.1"
                     /translation="MENKEKKNLQKYWQKIYQKIRDITNLHEDTNIETTLESIKKDVE
                     FKGANVWILFFAVIVCSVGLNINSTAVIIGAML"
BASE COUNT          808 a          308 c          399 g          764 t
ORIGIN      
        1 tttctgttga tggattagtc tttactaatc atcattgtgg atttaatgcc atacaacagc
       61 atagtaccat agagcataat tatttaaaaa atggctttac tgctcaaaaa ttagaagatg
      121 aaattcctac accaggtctc acagttacat ttttggtaag aattgaagat gttaccaaca
      181 atattttatc tcaaattccc gattctttaa ccggtataaa ccgtgaagat aaaatttcaa
      241 ttatttctga tagcctttct catgcagcga taaaagattc ttcttataca gcaaaagttc
      301 ttcctttctt taatgagaat cagttttatc tttttgttta tgagaagttt gaagatgttc
      361 gtttagcctt tgcacctcca agttcaatag gaaaattcgg tggcgatacc gataattgga
      421 tgtggcctcg ccacacaggc gatttttctg ttttcagggt ttattgttca cctgacggca
      481 aacctgctac ttattctacg gataatgttc cttattcacc caaacgcttt atccctattt
      541 ccaacaaagg atataaagaa gatgacttta ctatgattct gggcaatcct ggctctacca
      601 gccgttattt gtcttctttt ggaattgact atcgaatgaa agtgcttaat caagctcgta
      661 ttgatgtacg tggaatcaaa caagatattt ggcgtagctt tatgaacagc aatgaagcta
      721 ttcaaattgc ttatgccaat aaatatgctt ccagttctaa ttattggaaa aacagtattg
      781 ggatgaatca agccattgta aaattgaaag tcatggaaag gaaaaaagca gaagaagctc
      841 aatttactga atggctaaaa acttcacctg aacgacagaa aaaatataat gatgtcttga
      901 cttcactgga agaaggatat gcaaaaatgt atccttatgg taaagctttg atttatatgt
      961 atgaatcgtt aattaatgga gttgaattac cgagaatagc tgaaaatata caacaatggc
     1021 ataatttcaa tttttccaat gatgaaatat taaaaaaagc cgaaaagctt tacaaggatt
     1081 attatccaga agttgatcaa aaagttttta ttgccatgtt gaaagtttat aaaaaatcag
     1141 ttgatgctga tgcccttcct gaaatttatg aagtaatcca gaaaaaattt aaaggagagg
     1201 aagaaaaata cgtgcaatat ctttttgata aatccagttt tacaacttat gagaaattga
     1261 agaaggcttt gttaaataat aaaatagatt ttaaaaatga tcctgcttta gttttttctt
     1321 ctgttgttaa acaaacatat aataacatta atttaaaact atctcctatc aatgatcaaa
     1381 taaaggaagt cgaaaaaatt tatatggctg gattaatgga aaaagctcag gaagaaggta
     1441 aacaacttta tcccgatgct aattttacga tgagaatgac ttatggaaaa gtagccggtt
     1501 atgttccggc tgatgccgta acctatcgtt attatacaac tactaaaggt attttagaaa
     1561 aagaaaatcc cagtaatgaa gaatttattg ttccttcttc tttaaaaaaa gctattgaga
     1621 acaaaaattt tggaaattat gcagataaaa acacaggtga aatgcaagta aattttatta
     1681 ctaacaatga cattactggt ggaaattcag gaagtcctat atttaatgca aagggcgaat
     1741 taataggttt ggcttttgac ggaaattggg aatcgttgag tggtgatatt atttttgaac
     1801 ctgatttgca gcgaactatt ggtgtagata tccgttatat gctttttgta atggacaaaa
     1861 taggtaatgc cgagcgatta ttgaaagaat taaatatttg taattgagag gaacttttta
     1921 gattcactgt ttgttgaatt ttagtgtttt aaaacaaaat tgttctttaa tttttttgaa
     1981 agcaaattgt gaagtgtcaa tttgtttaga aatgtaactt ggtttatatg tttaaatctt
     2041 aaagttatgg aaaataaaga aaagaaaaat ttacagaaat actggcaaaa aatttatcag
     2101 aagataagag atattaccaa tcttcatgaa gatactaata ttgagactac tcttgaatct
     2161 ataaaaaaag atgtagaatt caaaggtgca aatgtctgga ttttgttttt tgctgtgatt
     2221 gtttgttcag taggattaaa tattaattca acagcagtga ttattggtgc tatgttaat
//
LOCUS       DAOTKS010000273         2279 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_38202_length_2279_cov_4.331835, whole genome shotgun sequence.
ACCESSION   DAOTKS010000273 DAOTKS010000000
VERSION     DAOTKS010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38202_length_2279_cov_4.331835"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="P5236_05630"
     CDS             complement(<1..370)
                     /locus_tag="P5236_05630"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HRU63879.1"
                     /translation="MRKIFQRIGFLIGKAIDFFYPPFRKYMTLQFFRYGITGAANMVF
                     DWVLYFIVYNFVLQHRMLHLGFVTMSAHIGALAITFPISFFTGFLLQKYVTFTTSYLR
                     GRVQLFRYLLVVMVNLGLNYV"
     gene            564..1343
                     /gene="rsmA"
                     /locus_tag="P5236_05635"
     CDS             564..1343
                     /gene="rsmA"
                     /locus_tag="P5236_05635"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRU63880.1"
                     /translation="MAEVKAKKYLGQHFLKDKNVARRIVESLPIQEKTSVLEIGPGMG
                     ILTQFLINQPLIDLVAIEIDHESVIYLHKHFPNLKVIEGDFLQLDLQKIFSGEFYIIG
                     NFPYNISSQIFFKLLENKDIIPSLTGMVQKEVAERLAALPGSKIYGILSVLLQAYYKV
                     EYLFTVDENVFQPPPKVKSAVIQLTRNENLNIDCNEKLFKTVVKTAFNQRRKTLRNSL
                     RPLVEKNHEIYKNSFFDKRPEQLDVAAFIQLTNQIEKAMKQ"
     gene            1435..>2279
                     /locus_tag="P5236_05640"
     CDS             1435..>2279
                     /locus_tag="P5236_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HRU63881.1"
                     /translation="MSELRIFYHEDGKLKISKNLDILKKVDLNNLIWIDLNDVPENVE
                     SELEQFLKIYIQEDEEIEEIEMSSRYIQTDDSIVANSNFLQDNYVMEPVSFIIKNGIL
                     VSVRSSELKSFNETVKKLFVNTSNFPTGYHVLVSIFETRIEYDADMIEEVTDQITNLS
                     KTLNSEDILDQNILILIKDLQEKVMLIRQNIVDKQRVISNMLKCNFFPRDLLSRLNMI
                     IKDINSLFEYTRFDFDRLDYLQDTFLGLANIQQNKIIKIFTVVSVIFLPPTLIASIYG
                     MNFKY"
BASE COUNT          850 a          332 c          339 g          758 t
ORIGIN      
        1 ctacataatt taaacccaaa ttaaccatta ccactaataa atatctaaac agttgtactc
       61 gccctcgtaa ataagaagtt gtaaaagtaa catatttttg tagtaaaaaa ccagtaaaaa
      121 aagaaattgg gaaagttatt gccaatgctc caatgtgagc actcatggtt acaaaaccca
      181 gatgaagcat tctgtgttgt aaaacaaaat tatacacaat aaaatataaa acccagtcga
      241 aaaccatatt tgcagcacct gtgattccat atctgaaaaa ttgcaaagtc atgtatttac
      301 gaaaaggcgg ataaaaaaag tcaattgctt tacctattaa aaaaccaatc ctttgaaaaa
      361 ttttcctcat tcattataag acataaaaat ctaaaatcgt attaataatt ttttaaaaaa
      421 gaagaagttt ctaacaattt taattacttt tgcagtagaa aatttcgagg ttacaaagat
      481 acaattttgt ttgttaatct taatttttca ttttttaatt aattttattg ggttgtttat
      541 catgtccgac gattatcaca gtaatggccg aagtaaaagc aaaaaaatac ttagggcaac
      601 attttttgaa ggacaaaaat gtggctcgcc gaattgtaga aagtttaccc atccaggaga
      661 aaacttcagt actggaaatt gggcctggga tgggtatatt aactcaattc cttatcaatc
      721 agcctcttat tgatcttgtc gctattgaaa ttgatcacga atcagtaatt tatctccata
      781 aacatttccc taatttaaaa gttattgaag gcgattttct tcaattagac ttacaaaaga
      841 ttttttctgg tgaattttat attattggca atttccctta caacatttca agccaaatat
      901 tttttaaact tcttgaaaat aaggatatta ttccatccct aacgggaatg gtgcaaaaag
      961 aagtagctga acggttggct gctttaccag gcagcaaaat ttacggtatt ttgagtgtgt
     1021 tgcttcaagc atattataaa gtagaatatt tatttactgt tgatgaaaat gtcttccaac
     1081 ctccgccaaa agtgaaatcg gctgttattc aacttactcg aaatgaaaat ttgaacatcg
     1141 attgcaacga aaaacttttt aaaacagttg taaaaacagc ttttaaccaa cgacggaaaa
     1201 cattgcgaaa ttcattgaga cctttggtag aaaaaaatca tgaaatatat aaaaactctt
     1261 tttttgataa acgtcctgaa caattggatg tagctgcatt tattcagctt accaatcaaa
     1321 tagaaaaggc aatgaaacaa taaccaaata ataatttttc attttgttgt tacactactt
     1381 actgctggat aatttaataa tccttcagta ataaaaaata gaaaggaggc aatcatgtca
     1441 gaattaagaa tattttatca tgaagatggt aaattaaaaa tttcaaaaaa tcttgatatc
     1501 ctcaaaaaag tagatcttaa taatttaatc tggatagatt tgaacgatgt tccagaaaat
     1561 gtagaatccg aattggaaca atttttaaaa atttacattc aggaagatga agaaattgaa
     1621 gaaattgaaa tgagttcccg gtatatccaa actgatgaca gtattgtggc aaactcaaat
     1681 ttcttacaag ataattatgt aatggagcca gtttcattta ttataaaaaa tggaattttg
     1741 gtttcggtac gtagttcaga attaaaatca ttcaatgaga ctgtaaaaaa gctttttgtt
     1801 aataccagta atttccccac aggttatcat gttttggttt cgatctttga aacccgtata
     1861 gaatatgatg cagacatgat tgaagaagtg actgaccaaa ttaccaactt aagtaaaact
     1921 ttaaatagtg aagatatcct ggatcaaaat attttaatac ttatcaagga tttacaagaa
     1981 aaagtgatgc ttatccggca gaatattgta gataagcaac gggttatttc caatatgtta
     2041 aaatgtaatt tctttcctcg tgatctttta tctcgtttaa atatgatcat aaaagatatt
     2101 aattctttat ttgaatatac acgattcgat tttgatcgat tggattattt gcaggatact
     2161 tttcttggat tagcaaatat tcagcaaaac aaaattatta aaatatttac tgttgtatct
     2221 gttatatttt tgcctcccac acttattgca agtatttatg gaatgaactt taaatatat
//
LOCUS       DAOTKS010000274         2260 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_38629_length_2260_cov_3.377324, whole genome shotgun sequence.
ACCESSION   DAOTKS010000274 DAOTKS010000000
VERSION     DAOTKS010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38629_length_2260_cov_3.377324"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            429..1877
                     /locus_tag="P5236_05645"
     CDS             429..1877
                     /locus_tag="P5236_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HRU63882.1"
                     /translation="MQEIKFYKGENIPLELHKVRIVQKLHLVPIERRLEALQEGGYNT
                     FRLNTKDVFLDMLTDSGTNAMSDNQMAAMMQADDAYAGSQSFYRLLKAVQDVLDKKYY
                     LPVHQGRAAENIISYAYVKKGSIIPMNYHFTTAMAHITQYGGKIAELLYNEAYVIDSS
                     HPFKGNMNLEKLEEIIQLHGPENIPFIRMEASTNLIGGQPFSVQNLRDVRAIADKYKI
                     RLVLDASLIGENAYLVKQREEEFKDASLGEIIKTMTGLADLVYFSARKLSSSRGGGIC
                     TNSDEIYHELEALIPLFEGFLTYGGMSVREMEAMAVGLYETLDETIICQSPNFIKYLV
                     QALDEKGIPVIKPAGVLGVHLDAMQICAHIPQKEYPAGSLAAALFLISGIRGMERGTI
                     SNQRDEYGNETYADMELLRLAIPRRVFTLSQIKYVEDRVNWLYDNRELIGGLRFVYEP
                     PVLRFFMGGLEPVNDWPQKLMAKFKEDFGDSL"
BASE COUNT          766 a          366 c          442 g          686 t
ORIGIN      
        1 gaaatgcaac aatggcaaaa accaaaaaag tagagattat ccagaaagat aaacagaaaa
       61 aataattgaa aagttgcgag gttgggagtt ttgaattgtt aatgtcacgt cctgaaaaaa
      121 gttgaccatt ttttgcttac tttttatgat aagataaaaa atatgtagcg tgtttgagta
      181 aatcccacat tattttggcc gacacaaaaa acgtttcaaa aaacgtggaa aatcaaataa
      241 aaagcacaaa atgaaaggat agagaaattg caaattaagc tgttcctcat cttatttatg
      301 aaatgatgaa atttcaatgg gaaaatgact aattttgcat tcaattaaaa ttattcttta
      361 cacacttttt agaatactac tgttttatat taaataaatt aacttctata ttattaatca
      421 tttttatcat gcaagaaatc aaattttaca aaggcgaaaa cattcctctc gaattacaca
      481 aagtaagaat agtgcagaaa ttacatcttg tgccaatcga aagacgtttg gaagctttgc
      541 aggaaggggg atataacacc ttcaggttga acaccaaaga cgtttttttg gatatgctaa
      601 ccgatagtgg caccaatgct atgagcgata atcaaatggc tgctatgatg caggctgatg
      661 acgcttatgc tggatcccag agtttttaca ggctgttgaa agctgttcag gatgttttgg
      721 ataaaaaata ttatctacct gttcatcaag gtcgtgcagc cgaaaacatt atttcatacg
      781 cttatgtaaa aaaaggaagc atcataccaa tgaactacca ttttaccaca gcaatggcac
      841 atatcaccca atatggtggg aaaattgccg aactgcttta taatgaagct tatgtgattg
      901 attcttcgca tcctttcaaa ggaaatatga atctcgaaaa attggaagaa attattcaac
      961 tacatggacc agaaaatatc ccgttcattc gcatggaagc atctaccaat ttgattggag
     1021 gacaaccttt ttcagtacaa aatttacgtg atgtgagagc tatagccgac aaatataaga
     1081 ttcgacttgt gctcgacgca agcctgattg gcgaaaatgc ttatctggtg aagcagcgtg
     1141 aggaagaatt taaagatgct tcattaggtg aaataataaa aaccatgacc ggtttagctg
     1201 atttggtata tttttcggct cgtaaactca gttcatcacg cggtggtgga atatgtacaa
     1261 acagtgatga aatttatcac gaattggaag cgctgattcc gctttttgaa ggatttctca
     1321 cctacggcgg catgtcagta cgcgaaatgg aagctatggc agttggactg tatgaaacat
     1381 tggatgaaac aattatctgt caaagtccta atttcattaa gtatcttgta caagcacttg
     1441 atgagaaagg cattccagtc ataaaaccag ccggcgtact cggtgttcat ttggatgcga
     1501 tgcagatatg tgcacacatt ccacaaaaag aatatcctgc cggatcactt gccgcggcac
     1561 tttttctgat ttcaggaatt cggggaatgg aacggggaac tatctcgaat cagcgtgacg
     1621 aatatggtaa tgaaacctat gctgatatgg aattgttgcg tttagctatt ccaagacgcg
     1681 tgttcacact atcccaaata aaatatgtcg aggaccgcgt gaattggctg tacgataatc
     1741 gtgagctcat cggtggactt cgatttgttt atgagccacc cgtacttcgt ttctttatgg
     1801 gaggtcttga gccggtaaat gattggccac aaaaacttat ggcaaaattt aaggaagatt
     1861 ttggcgatag tttgtaatta taataggttg ttttattttt tgaaacagcc tcttgtctta
     1921 aaaaaatatt ttattaacct tttatttccc ctgaaaaagt tgcattaatt agttgaattt
     1981 acgaacaata ggtgaacttt tcagggcaat gaatactgaa tgtttacagc aaacacttga
     2041 caaatgtctt tggaaattat ttttataact gaaatatatg aagcattaga aaaggatatt
     2101 gatgaactat ttgtattgat catgaactac cctaaaacag cagagctggt tttgaaaaat
     2161 atctgctcct tcgctacaga aaaaagtttg agtaaatata attgttaata tattgataat
     2221 cagggtggaa atagtcaaat cgaacttcat tatttcttgt
//
LOCUS       DAOTKS010000275         2259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_38652_length_2259_cov_4.674229, whole genome shotgun sequence.
ACCESSION   DAOTKS010000275 DAOTKS010000000
VERSION     DAOTKS010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38652_length_2259_cov_4.674229"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..1946
                     /locus_tag="P5236_05650"
     CDS             66..1946
                     /locus_tag="P5236_05650"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HRU63883.1"
                     /translation="MPIKKHLVFCILFFFTNSIWGTIPVKPRPVKTWKISDPFAVVDT
                     IPVDTLHLNFQDANPIDRFSIANSYNGNLGSPIEPKLYFDRPEKSDFIFSEAFYPYIM
                     DIEKATFYNVKMPYTHIVYNTGGSTYRQQDHIKFLFTANANKSLNFGTNLDYFYAVGE
                     YNNQAAKRFSGSLFGSYNGKHYSATGLVTVNNMFNHENGGIADSSYIYEPLGIATKDI
                     PTRINGYSVYRKNVFFYNQQYTIGFERPVHISPDSVKMEYVPVTRFAHTIKYEDPRKR
                     YYEPTVEKDFYQNTYLPFTQTNDTAALQSLTNTVSVNMAEEFNKWLKFGMTAFLQNEV
                     QRYTFKIDSVLERKNMSSTKVGGILSKYKGPRFRYEILGEMGLLGYKTGDFQIKGKLG
                     GYFKLFKDSIILTAGGFVKSDEPSYFLQHYHSNHFCWDNNFNKTYRTNINGTFSIPTR
                     YFSLNVSVENLTDYIYFNSQALPVQHNGNVQVLALNLKKDFHFGKFNLENNIVYQRSS
                     NSEVIPLPAFALYHNLYYNGLWFNVLSAQLGANLRYFTEYYSPCYMPATAQFYNQSTT
                     KVGNFPVINVYANFHLKQARFFVEYYHINQLFMKTAYFSMPNYPLSPAIFKIGVSTNF
                     YN"
     gene            2010..>2259
                     /locus_tag="P5236_05655"
     CDS             2010..>2259
                     /locus_tag="P5236_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63884.1"
                     /translation="MKIKKYIFFTIAFVLLILFIKLLFFREKTAHNLPAILKSGRLSV
                     VTDSSALGFAMKGDSVFGFQYEIAKAFADSLNLELIIST"
BASE COUNT          733 a          346 c          358 g          822 t
ORIGIN      
        1 gatatttttt gtaattttgc tatcgtttta aatttttggc aggtaatttc ttaactatca
       61 ttttaatgcc tataaaaaag catcttgttt tttgtattct ttttttcttt actaatagta
      121 tttggggaac aattcctgta aaaccacgtc cggtaaagac atggaaaatt tctgatcctt
      181 ttgctgtggt agatacgatt ccggtagata ctttacacct taattttcag gatgctaatc
      241 ccattgatcg tttcagtatt gccaattcat ataatggaaa cttgggttct cctattgaac
      301 caaaactgta ttttgatcgc cctgaaaaaa gcgattttat tttttcagaa gctttttatc
      361 cttacataat ggatatagaa aaagcaactt tttataatgt aaaaatgcct tatactcata
      421 ttgtttacaa taccggtgga tccacatatc gccaacaaga tcatataaaa ttccttttta
      481 ctgccaatgc caataaatca ctgaattttg ggacaaacct ggattatttc tatgcggtcg
      541 gcgaatataa taatcaagca gcaaaacgct ttagcggctc attatttggc agttataatg
      601 gcaaacatta tagtgctact ggcttagtga ctgtaaacaa catgttcaat catgaaaatg
      661 gagggattgc cgattcttct tatatatatg aacccttagg aatagctacc aaagatattc
      721 ctactcgaat taatggatat tcagtttata gaaagaatgt ctttttttat aatcaacaat
      781 acacaattgg atttgaacga ccagttcata tttcaccaga ttcagtaaaa atggaatatg
      841 tccctgttac gcgatttgcc catactataa aatatgaaga tcctcgaaaa agatattatg
      901 aacctacagt agaaaaagat ttttaccaga atacttatct gccttttaca caaaccaatg
      961 atacggctgc tttgcaatcg ttgaccaata ctgtatcggt aaatatggct gaggagttta
     1021 ataaatggtt gaagtttgga atgactgctt ttttacagaa tgaagttcaa agatatactt
     1081 ttaaaattga ttcagtttta gagcgaaaaa atatgtcgag tacaaaagtt ggcggaattc
     1141 tttcaaaata caaaggacca cgttttcgtt atgaaatttt aggcgaaatg ggattgctgg
     1201 gatataaaac tggagacttt caaatcaaag gtaaattggg tggctatttt aaattgttta
     1261 aagacagtat tattttaacg gcgggtggat ttgtaaagag tgatgaaccc tcttattttt
     1321 tgcaacatta tcattccaac catttttgct gggataataa ttttaataaa acctaccgta
     1381 ccaatattaa tggaaccttt tccataccaa ctcgctattt ttcactcaat gtttctgttg
     1441 aaaatttgac tgattatatt tattttaatt ctcaagcatt gccggtccaa cacaatggaa
     1501 atgtacaagt tctggcattg aatttaaaaa aggattttca ttttggaaaa tttaatttgg
     1561 agaacaatat tgtttatcag cgaagttcaa attcggaagt aattccactt cctgcatttg
     1621 ccttgtatca taatttatat tataatggct tgtggttcaa cgtgctaagt gctcaattag
     1681 gcgctaattt acgttatttt acagaatatt attcaccttg ttatatgcca gctacagcac
     1741 aattttataa tcagtccaca acaaaagtag gaaattttcc agtaattaat gtatatgcca
     1801 actttcattt gaaacaagct cgtttttttg tagaatatta tcatatcaat cagttgttta
     1861 tgaaaactgc ttatttttct atgcctaatt atcccctttc tccagcaatt tttaaaattg
     1921 gggtttctac caatttttat aattaaaagg agaaaccagt atttttaaaa ctttcgattg
     1981 atttattcat ttaaaatagt tacttattta tgaaaattaa aaagtatatt ttctttacaa
     2041 ttgcttttgt attattaatt ttatttatta aactgttgtt tttcagagaa aagacagcac
     2101 ataatttgcc agctatttta aaatcgggaa gattgtctgt tgtcactgac agcagtgctc
     2161 ttggttttgc aatgaaagga gacagtgttt ttggatttca atatgaaatt gcaaaagctt
     2221 ttgctgatag tttgaatctt gaattaataa ttagtacta
//
LOCUS       DAOTKS010000276         2254 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_38763_length_2254_cov_3.864484, whole genome shotgun sequence.
ACCESSION   DAOTKS010000276 DAOTKS010000000
VERSION     DAOTKS010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38763_length_2254_cov_3.864484"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="P5236_05660"
     CDS             complement(<1..472)
                     /locus_tag="P5236_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005835969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HRU63885.1"
                     /translation="MNLSDQLIDWYQVHKRDLPWRNISDPYQIWISEIILQQTRVNQG
                     LDYYLRFVERFPNVEMLAAAEEDEVLKYWQGLGYYSRARNLHQAARQIIQDFGGVFPK
                     TYPEVLRLKGVGTYTAAAICSFAYNLPYAVVDGNVYRVLSRLFAIETPIDSHSGK"
     gene            complement(676..>2254)
                     /gene="metG"
                     /locus_tag="P5236_05665"
     CDS             complement(676..>2254)
                     /gene="metG"
                     /locus_tag="P5236_05665"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRU63886.1"
                     /translation="YGDQCESCGSSLNPTDLIHPRSAITGNIPVLRETKHWYLPLDQH
                     EEWLRHWILEEHKEWKPNVYGQCKSWLDMGLQPRAVSRDLDWGIPVPLEGAEGKVLYV
                     WFDAPIGYISNTKELLPDSWEKWWKSEDTRLIHFIGKDNIVFHCIIFPTILKLEGTYI
                     LPDNVPANEFLNLEGDKISTSRNWAVWLNEYLEDFPGKQDTLRYVLTANAPETKDNDF
                     TWKDFQARNNNELVAIYGNFVNRTLVLTQNYFGGKVPARNELQGIDIQAINELSEVKK
                     NVEKLLDNFKFRDAQKEAMNLARLGNKYLADTEPWKVAKTNKDRVATVLNISLQIAAN
                     LSIVFEPFLPFSSAKLRDLLNLETFSWDSLGSIELLKPQHQLKKPELLFEKIDDAAIE
                     YQVQKLLNTKKKNELSSFKAKSVKSNISYEDFEKLDIRIGTVLACQKVPKADKLLQFK
                     IDDGLGGRTIVSGIAQYYPEPEKLVGQQVCFIANLEPRKLKGIVSEGMLLSAEDADGS
                     ISLIQPQTKVVNGAEVK"
BASE COUNT          672 a          491 c          400 g          691 t
ORIGIN      
        1 ttttacctga atggctgtcg ataggcgttt cgatagcaaa aaggcgtgaa agaacgcgat
       61 aaacgtttcc atctacaacg gcataaggaa gattgtaggc aaaagaacaa atagccgctg
      121 ccgtataagt tccaacacct ttcaagcgta aaacttccgg ataagttttt ggaaaaacac
      181 cgccaaaatc ctgtataatt tgtcgtgctg cctgatgcaa atttcgggca cgcgaataat
      241 aacccaaacc ttgccagtat ttcaaaactt cgtcttcttc agcggcagca agcatttcaa
      301 cattcgggaa acgttcgaca aaccgcaaat aataatccaa cccttgattg acacgcgttt
      361 gctgcaaaat gatttcggaa atccaaattt ggtaaggatc agaaatgttt cgccatggca
      421 aatcacgttt atgaacctga taccagtcga taagttgatc ggataaattc atatgaaaga
      481 acaaaaaact tgcgaattgg aggaaatatt aaaacaacaa acaacccaaa ttgaaattta
      541 cttcacatca aatgctattt acatagcctt cccctaaaac ctctttcatt tttcattatt
      601 gctgtatttc tgaatttttt ttcaaaatat tcaaagcttt aaattcagaa ctcgaaactc
      661 atcactcgaa actcatcact tcacttcagc tccatttacc acttttgttt gaggctgaat
      721 caatgatatg ctgccatcag catcctctgc cgagagtagc attccttcac tgactattcc
      781 ttttaatttg cgtggttcaa gattggcaat aaaacaaacc tgctgtccaa ctaatttttc
      841 gggttcaggg tagtactgag caatacctga aacaatggta cgcccgccca agccgtcatc
      901 aattttgaat tgaagcagtt tgtcggcttt aggaactttc tgacaagcca acaccgttcc
      961 gatacgaata tcgagttttt caaaatcttc gtaagaaata ttggatttta cggatttagc
     1021 cttgaacgac gaaagttcat tctttttctt cgtattcaag agtttttgca cttgatattc
     1081 gatagcagca tcatctattt tttcaaaaag aagttccggt tttttcaatt ggtgttgagg
     1141 tttcagtaac tcaatacttc ccaaactatc ccagctgaaa gtttccaaat tcaataaatc
     1201 tctcagcttt gccgagctga aaggcaagaa aggttcaaaa acaatagaaa gatttgcagc
     1261 tatctgtaaa cttatattca aaacagtagc aactctgtcc ttatttgttt ttgcaacttt
     1321 ccatggttct gtgtcggcaa gatatttatt tcccagccgg gcaagattca tggcttcttt
     1381 ctgagcatca cggaacttga aattatcgag caatttttca acatttttct tcacttccga
     1441 caattcgtta atggcctgta tatctattcc ctgcaattca tttcgcgccg gaacttttcc
     1501 tccaaaataa ttttgtgtca gcacaagggt tctatttaca aaattgccgt aaatagctac
     1561 caattcattg ttgtttcgtg cctgaaaatc tttccatgta aagtcgttat ccttcgtttc
     1621 gggagcatta gctgtcagca cataacgcag agtatcctgt tttccgggaa aatcttcgag
     1681 atattcgttc aaccaaactg cccaattgcg ggaagttgaa attttgtcgc cttcgaggtt
     1741 tagaaattcg ttggccggaa cattatccgg taaaatgtaa gttccttcca gttttaagat
     1801 tgtggggaaa ataatacagt gaaaaacaat attatctttt ccgataaaat gaattaacct
     1861 tgtatcctca cttttccacc atttttccca actgtctggc aaaagttctt tggtgttgga
     1921 aatatagcca atgggagcat caaaccacac atacaatact tttccttcgg cgccttcgag
     1981 tggaacagga atgccccaat ccaaatcgcg acttacggca cgtggttgca aacccatgtc
     2041 gagccagctt ttgcattgtc cataaacatt tggtttccac tctttgtgtt cttccagtat
     2101 ccaatgtcgc aaccattctt cgtgctgatc gagaggcaaa taccagtgtt tggtctcacg
     2161 caaaaccgga atatttcctg taattgctga gcggggatga atcaaatcag taggattgag
     2221 cgaagaaccg caagattcac actgatcacc gtaa
//
LOCUS       DAOTKS010000277         2252 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_38827_length_2252_cov_2.800182, whole genome shotgun sequence.
ACCESSION   DAOTKS010000277 DAOTKS010000000
VERSION     DAOTKS010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38827_length_2252_cov_2.800182"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="P5236_05670"
     CDS             complement(<1..373)
                     /locus_tag="P5236_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRU63887.1"
                     /translation="MDRRDFLRAIALTGAALTTIKESKAMSILTQSFQTNNSTTKYDL
                     VAVMGGEPEEMFRKAIAEMGGIENFIKQGDKVCVKPNIGWAQPPEMGADTNPKLVAEI
                     IKQCLKAGAKEVIVFDHPCDEW"
     gene            complement(493..891)
                     /locus_tag="P5236_05675"
     CDS             complement(493..891)
                     /locus_tag="P5236_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRU63888.1"
                     /translation="MTEKSWEEILQRFREIEFQETFDIIVAIANGGIIPAAILNQRLK
                     IEIQLLKINLRDAERKPIYDIPKLLSPINFNYVDKKILLVEDRIKTGATVQFAIELLK
                     DAAQIKTFAVNGKADYSLYNEPCFRFPWQL"
     gene            complement(903..1412)
                     /locus_tag="P5236_05680"
     CDS             complement(903..1412)
                     /locus_tag="P5236_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRU63889.1"
                     /translation="MENTIKLYSFNLSNLKTYLFAAIFVAGNLLLPQLVHLIPNGGLI
                     FLPIYFFTLIAAYKYGIHVGLLTAILSPLVNSLLFGMPPMNMLPIILIKSVILAIAAA
                     MVAKHFGKISFLGILLVILAYQIIGTGIEWAMTQNFFIAIQDFRIGLPGMVIQLVGGY
                     FVLKAIAKI"
     gene            2065..>2252
                     /locus_tag="P5236_05685"
     CDS             2065..>2252
                     /locus_tag="P5236_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRU63890.1"
                     /translation="MIFLLFLFSLSIGITYAQTRVTGRVIDEVGEPVIGASIKVKGTT
                     VGTISDNNGNFTLIVPENA"
BASE COUNT          808 a          310 c          325 g          809 t
ORIGIN      
        1 gccattcatc gcaaggatga tcaaaaacaa tcacttcttt ggcaccagct tttagacatt
       61 gttttatgat ttcagctacc agttttggat tcgtatcagc tcccatttct ggtggttgag
      121 cccaaccgat atttggtttt acacatactt tatcaccttg ttttatgaag ttttctattc
      181 cgcccatttc ggcaatagct ttgcggaaca tctcttcagg ttcacctccc atgacagcta
      241 ccaaatcgta ttttgtagtt gaattatttg tttgaaacga ttgggtaaga atactcatcg
      301 ctttactttc ttttatagtc gttaatgctg ctcctgttaa ggcaattgct cgtaaaaaat
      361 cacgcctatc cataatttgt tttattttga aataatttta gttacaagtt aaattaagat
      421 attaattgat tgattattaa tttttttaat gaatcaagaa aaacattaaa aatctattat
      481 gaaatgttca gtttatagtt gccacggaaa tcgaaagcag ggttcattat aaagtgaata
      541 atcagcttta ccatttactg caaaagtttt gatttgagca gcatctttta aaagttcgat
      601 ggcgaattgt acagttgccc ctgttttaat acgatcttcc accagaagaa tttttttatc
      661 tacataatta aaattgatcg gagacaaaag ttttggaata tcatatattg gtttccgctc
      721 tgcatctcgt agatttattt ttaaaagttg aatttcaatc ttcaatcgtt ggttgagaat
      781 agcagcaggt atgatgccac cattcgcaat ggcaacaatt atatcaaaag tttcctgaaa
      841 ttcaatttca cgaaaacgtt gcaaaatttc ttcccatgat ttttctgtca tcggactcaa
      901 ttttatattt tagctatggc tttcaaaaca aaataaccac ctacaagctg aatcaccatt
      961 cctggtaaac ctattcgaaa atcttgaata gcgatgaaaa aattttgtgt catagcccat
     1021 tctatgcctg tgcctataat ttgataagca aggattacca acaaaattcc taaaaaagat
     1081 atttttccaa aatgcttagc aaccattgca gcagcaatag ccagaataac agatttaatt
     1141 aaaataattg gcagcatgtt cataggtggc ataccaaaca aaaggctatt cacaagtgga
     1201 gataaaatag ctgttaacaa tccaacgtga ataccatatt tataagcagc aatcagcgta
     1261 aagaaataaa taggcagaaa tataagtcca ccatttggaa taagatgcac caattgtggt
     1321 aagagtaaat ttccagcaac aaaaatcgct gcaaaaaggt aggtttttaa attgctcagg
     1381 ttaaaagaat acagttttat agtattttcc atgttttaaa atttgaagtt gattaaacat
     1441 ctttactaca aaactataaa taattgattg aatatttcct taggtacgct tattattttt
     1501 atttaaaaca tataaaattt attaataatt tgtaattttc taagattaaa aactgagcag
     1561 aaatttataa aatttcattg tatagacaaa atatgtaaat aatcataaat aaattttttt
     1621 tactaaaaaa taaatgtgat taaattaatt ttttactatc tgaaataaaa aggttattgg
     1681 tttgataaag aaaaagttaa tggttaaatt tgtttgaatc ttttatttta caattttttg
     1741 atgtatgaag tgaatttagc tattttttat gatatcaaaa taaaatattg ttaaactttg
     1801 tttatttttg aagaatattc agaaaatgat tttatattta catttatttt aaaaaatctc
     1861 ttttaataaa gtcctaattt gtaaattact gaatatcaat gctaaatttt aaaagaaatt
     1921 aataaaaatt ggttttttaa aataaattca tattaattaa taaataaaaa aaattcttat
     1981 atttattatt tttagtgtat agttattaaa taaaacaaat gtttttcaat gatttacaaa
     2041 aaacaaatga gtatgaataa aaaaatgatt tttttgttat tcctatttag tttgagtatt
     2101 ggaataacat atgcacaaac ccgagtgacg ggaagagtga tagatgaagt gggtgaacct
     2161 gttattgggg catccattaa agttaaaggt accactgtgg gaactatttc agacaataat
     2221 ggaaatttta cgttaattgt tccggaaaat gc
//
LOCUS       DAOTKS010000278         2248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_38911_length_2248_cov_4.206110, whole genome shotgun sequence.
ACCESSION   DAOTKS010000278 DAOTKS010000000
VERSION     DAOTKS010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38911_length_2248_cov_4.206110"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..961
                     /gene="rpsB"
                     /locus_tag="P5236_05690"
     CDS             125..961
                     /gene="rpsB"
                     /locus_tag="P5236_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRU63891.1"
                     /translation="MSRTNFEELLEAGCHFGHLKRKWNPAMAPYIFMERNGIHIIDLY
                     KTIAKIDEAAEALKQIAKSGRKILFVATKKQAKEIVAEKAKAIGMPYITERWPGGMLT
                     NFPTIRKAIKKMATIDKMMTDGTFDNISKREKLQITREREKLEKNLGSIADLTRLPAA
                     IFVVDVMKEHIAVKEAQRLEIPIFAIVDTNSDPTGIDYVIPANDDATKSIEVILNALV
                     AAIQEGLDERKLMKVEAEASEAPVVKEKKSRINRRPRTQKEDEEALKANVLNKFIKDE
                     EE"
     gene            1099..1917
                     /gene="tsf"
                     /locus_tag="P5236_05695"
     CDS             1099..1917
                     /gene="tsf"
                     /locus_tag="P5236_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRU63892.1"
                     /translation="MAVTMNDITKLRAMTGAGLMDCKQALIEANNDFEKAIEIVRKKG
                     QAIAAKRSSREALEGCVLAEAKDDFAVALSLKCETDFVARNADFIALTKAILQAALEN
                     RPTTKEELLALKINGRTIADLVIERSAVTGEKVELEDYVFVKGGSTAAYTHFGNRLAS
                     IVAFKEKDVDPKVAHEIAMQIASMNPIAIDRASVPQKMIETELEIAREKAREEGKPEN
                     MIDKIAEGRLNKFFQEAVLLEQTSIMDNKTIIGNYLKAHNATVIDFKRISLNQE"
BASE COUNT          844 a          321 c          421 g          662 t
ORIGIN      
        1 cagaaaagtt ttcatgcaaa ggaatcaaaa agtagtttag tatctaaatt ggcgagactc
       61 ttttatgaaa aaaggctact cgttgattga ttttaataga aggtaaacaa ttaaaaacaa
      121 aaaaatgtca agaacaaatt ttgaagaatt attagaagca ggttgccatt ttggtcactt
      181 aaaaagaaaa tggaatccag caatggctcc ttatattttt atggagcgaa atggtattca
      241 cattattgat ttatataaaa ccatcgcaaa aattgatgaa gcagcagaag ctttaaagca
      301 aattgcaaaa tctggaagaa aaattttatt tgtcgccaca aaaaaacaag ctaaagaaat
      361 tgttgcggaa aaggcaaaag ctataggaat gccttatatt actgagcgtt ggccaggagg
      421 aatgttaact aacttcccga ctatacgtaa agccattaaa aaaatggcta ctatcgataa
      481 gatgatgacc gatgggacat ttgataatat ttcaaaacgt gaaaaattac aaattactcg
      541 tgaacgagaa aaattagaaa aaaatctggg ttcgatagct gatttaacac gacttcctgc
      601 tgccattttt gtagtggatg taatgaagga acacattgct gtaaaggaag cccagcgatt
      661 agaaattccc atttttgcaa ttgttgatac taattctgat ccaacaggta ttgattacgt
      721 tattccagcc aatgacgatg caacaaaatc tattgaggtg attttaaatg cattggttgc
      781 agcaatacaa gaaggtctgg atgaacgtaa attgatgaaa gttgaagctg aagcttcgga
      841 agcgccagtt gtgaaagaaa agaaatcaag gattaatcga cgaccaagaa ctcaaaagga
      901 agatgaagaa gctttgaagg caaatgtatt aaataaattt atcaaagacg aagaagaata
      961 agcttaatga taatttaaac aaatcctctt taaacgagtt acaggttatc attatttcat
     1021 agctttgtga ctcgttttta aattaaataa ccatttattt ttttaattag tattttagaa
     1081 aggagaatat tgagaactat ggcagtaaca atgaatgata ttacaaaatt gcgtgctatg
     1141 acaggtgctg gtttaatgga ttgcaaacaa gcacttattg aggcaaataa tgattttgaa
     1201 aaagctattg aaatcgttag aaaaaaagga caagccattg cagccaagcg tagcagtcgt
     1261 gaagcattag agggttgtgt attggcagag gcaaaggatg attttgcagt tgctttgtct
     1321 ttaaaatgtg aaactgattt tgttgcaaga aatgctgatt ttatagcgct aacaaaggca
     1381 atattgcaag cagctttaga aaatagacct actactaaag aagaactttt agctttaaaa
     1441 attaatggac gaaccatagc cgatttggtt atagaacgtt cagctgttac aggagagaaa
     1501 gttgaattag aagattatgt tttcgttaaa ggtggttcca ccgcagcata tactcatttt
     1561 ggaaatcgct tagcttccat tgtagccttt aaagaaaaag atgttgatcc caaagttgct
     1621 catgaaatcg ccatgcaaat tgcttcaatg aatcctattg ctattgatcg tgcctcagtg
     1681 cctcaaaaaa tgattgagac agaactggaa attgctagag aaaaagctcg tgaggaaggg
     1741 aaacctgaaa atatgattga taaaatagct gaaggtcgct taaataaatt tttccaagaa
     1801 gctgttttac tcgaacaaac ttctatcatg gacaataaaa cgattattgg caattatctg
     1861 aaagctcaca atgcaacagt gattgatttt aaacgcataa gtctaaatca ggaataaata
     1921 tctattttcc cctcttttta ggaaaaaccg ctggatttta tcagcggttt tttattacac
     1981 atctcatatt acaatacctg ataataaaat tccatcacta caaataataa cgaaaaataa
     2041 ttcaaaaaga attaggaatt ccgacttttg aatggagaca ttttgtgaaa tatctgaata
     2101 tgcaaacaaa aaatccctgt ggttctcagc gtaataaact attcattttt ttaatttgct
     2161 ataatgaatt ggaaagtaat ttttatattt ccccgtaaat tcaagtaatt aatgcaactt
     2221 tttcagggga aataataagg taaaggaa
//
LOCUS       DAOTKS010000279         2244 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_38997_length_2244_cov_6.141617, whole genome shotgun sequence.
ACCESSION   DAOTKS010000279 DAOTKS010000000
VERSION     DAOTKS010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38997_length_2244_cov_6.141617"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..>2244)
                     /locus_tag="P5236_05700"
     CDS             complement(212..>2244)
                     /locus_tag="P5236_05700"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRU63893.1"
                     /translation="HIGVIKALEENGIPIDYVAGTSMGAIVGSMYAMGFTPDEMMEII
                     TSEDFKYWLRSEEEPEYIFYYRKSDLTPPLMEFSFRLPKQDTAKIKTKFLPTHLVPSH
                     QMNYAFLSLFSQPTAASKGDFNRLFVPFRCVASDIYKKEPVIFSKGSLGDAVRASMSF
                     PLVFKPVTIDSCLLFDGGIYNNFPVDVMRDDFHPDIMIGSVVTKNPQKPTEEDIILQL
                     ENMVMAKTDYTIPPEEGVLFQFDLKNINMFDFSKADLLFKMGYDSVMTRIEEIKSRIA
                     RRVPLEEVNKRRKEFRGNFPDLKFQNIIIEGVDSLQKIYIEREFHNKNEVFDLNQFKI
                     NYFKLLSDNQISEITPSAHYNQKNDCFDLHLKVKTEDHLKVTMGVNISSSVSNQAYVG
                     ASYQNLKEYAQTAYLEAQFGKIYNSLNIGTRWDASLKKDFYLKMDIILHRFDYFEGNK
                     LFYLDNRQAFFSQSEMFGKIKIGFPLTQKGRMELGIGFGNLTDNYLQDKFSTSSQKKN
                     DKSKFLLGNAFVRAETNTFTRPTYPTKGYHYFASLQLLGGDESFTFADPLRYNMENSQ
                     LWGQFQGKYDRYFPLSKKVVLGTYGEIVFSTRNLLPTYTATIMQAPAFQPTLHSLVIF
                     NEAFRANQFGALGLKPIYEFNDQIHFRTEMYCFVPYKKIFDSPQNYLLLEII"
BASE COUNT          743 a          384 c          331 g          786 t
ORIGIN      
        1 ttatatagtt gaaagaaaac aacttccgaa tcgcaactaa ttaaaacatt taagtttttt
       61 gctaacattg gatatacttt tctcgctgat ttgcgctgta tttttttctg tgttcatctg
      121 cgttttttat ctgcggttat ctgcgtgaaa tattcttcat tttctatttt tgaagcgagg
      181 ttctgcagtt tccctaaatt cagtatttca atcagataat ttctaataat aggtagtttt
      241 gcggagaatc aaaaattttt ttatatggta caaaacaata catttccgta cgaaaatgaa
      301 tttgatcatt gaattcataa ataggtttta aacccaaagc tccaaactga ttagctctaa
      361 aagcctcatt aaaaataaca agactatgaa gtgttggttg aaacgcagga gcttgcataa
      421 tggttgctgt ataggttggt aataagttac gggttgaaaa tacaatttct ccgtaagttc
      481 ctaaaacaac ttttttggaa agggggaaat atcggtcata ttttccttga aattgtcccc
      541 aaagttgact gttttccata ttataacgta atggatcagc aaacgtgaaa ctttcatccc
      601 cccctaacaa ttgaagagaa gcaaaataat gatagccttt tgttgggtaa gtgggacggg
      661 taaaagtatt ggtttctgcc cgtacaaaag catttcctaa taaaaattta cttttatcat
      721 tctttttttg agaggaagta gaaaatttat cctgcaaata attgtcggtt aaatttccaa
      781 accctatccc taattccatt cgtcctttct gtgttaaagg aaaaccaatt tttattttcc
      841 caaacatttc actctggctg aaaaaggctt gtctattatc caaataaaat aatttatttc
      901 cttcaaaata gtcaaatcta tgtaagatga tatccatttt taagtaaaaa tcttttttta
      961 aagaagcatc ccatcgtgtc ccaatgttca atgaattata aatttttcca aactgagcct
     1021 ctaaataagc tgtttgtgca tattctttca aattctgata agaagctcct acataagcct
     1081 gattacttac acttgatgag atatttacac ccatcgttac ttttaaatgg tcttctgttt
     1141 taacttttaa atgtaaatca aaacaatcat ttttttgatt ataatgagca gatggagtaa
     1201 tttcagatat ttgattgtca gataataatt taaaataatt tattttaaat tggtttaaat
     1261 caaacacttc atttttatta tgaaattccc tttcgatata aattttttga agtgaatcta
     1321 ctccttcaat gataatattc tgaaatttta aatctggaaa attcccacga aattctttac
     1381 gccgtttatt gacttcttcc aaaggaacac gacgagctat tctgcttttt atttcttcaa
     1441 ttcttgtcat aacggagtca tatcccattt taaataataa atcagcctta ctaaaatcaa
     1501 acatatttat gttttttaga tcaaactgaa ataagacacc ttcttctgga ggaattgtat
     1561 aatctgtttt agccatcacc atgttttcca attgcaatat tatatcttct tcagttggtt
     1621 tttgaggatt cttagttact acactaccaa tcataatatc aggatgaaaa tcatcacgca
     1681 taacatccac gggaaaatta ttataaatac ctccatcaaa taataaacaa ctatcaattg
     1741 tcactggctt aaacacaagg ggaaaactca ttgaggctct tacagcatcg cctaacgatc
     1801 cttttgaaaa aatcaccggc tcttttttat aaatatcaga agctacgcaa cggaagggaa
     1861 caaataaacg attaaaatca ccttttgaag ctgctgtggg ttgtgaaaac aaagatagga
     1921 aggcataatt catttgatga gaaggaacca aatgagtagg taaaaattta gttttaattt
     1981 ttgctgtatc ttgtttgggg aggcgaaagc taaattccat taacggagga gttaaatcac
     2041 ttttgcgata ataaaaaata tattcgggtt cttcttcgct tcgcaaccaa tatttaaaat
     2101 cttctgaggt aataatttcc atcatttcat ccggtgtaaa acccatggca tacatactac
     2161 ctacaatagc tcccatagag gttcctgcaa cataatcaat cggaatgcca ttttcttcta
     2221 atgccttgat tacaccaatg tgag
//
LOCUS       DAOTKS010000280         2237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_39141_length_2237_cov_3.736939, whole genome shotgun sequence.
ACCESSION   DAOTKS010000280 DAOTKS010000000
VERSION     DAOTKS010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39141_length_2237_cov_3.736939"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..906
                     /gene="proC"
                     /locus_tag="P5236_05705"
     CDS             103..906
                     /gene="proC"
                     /locus_tag="P5236_05705"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRU63894.1"
                     /translation="MNLENLKIAVIGAGNMGSALLHGWIKSKSISPQNISISDISKEK
                     LEKITTFESGVNVSLSNKEVIKKAKIIILAVKPWLIEEIAEEIKNEINYKEQILISIA
                     AGINFEKLLKIFDRKAMLFRVVPNTAAEVSESLCAISSCNADSEQQQLIMNLFNCIGK
                     AFLVPENQLNAFMSLTSCGIAYVFRYIRAAMEGGVEIGISPEICQQAVVQTLKGAVAL
                     LEANQTHPEIEIDKVTTPGGITIKGLNEMEANGFSNAVIQGIKASWLKD"
     gene            938..1486
                     /locus_tag="P5236_05710"
     CDS             938..1486
                     /locus_tag="P5236_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HRU63895.1"
                     /translation="MNEQIRQIALRLRGLRESLDLTISEAAEKCGVPKKDYEKYESGE
                     TDIPVSFICKVAETFGIETTALISGEEPRAQVFFVTRKGTGASIERTKAYKYQALAYG
                     FRNAKVDPFEVMVEPNNLPIYLNTHKNQEFNYVLEGTLLLEIDGKQLTLYEGDSIYFD
                     ATKPHGMKALNDKKVRFLAIII"
     gene            1610..>2237
                     /locus_tag="P5236_05715"
     CDS             1610..>2237
                     /locus_tag="P5236_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRU63896.1"
                     /translation="MLEKFVKQTEFKDFEDFNKNFQLIVPDHFNFAYDVVDEWAKTNP
                     NKQAMVWTNDQRECRKFTFADMKKYSDQTASYFQSLGIKKGDIVMLILKRRYEFWFSI
                     LALHKIGAIAIPATHLLTSKDLIYRNNAADIKAIVAVGEEPILTHINESLPDSTTVKF
                     LISIGPKVPENWLDFHKGIKEAAPFIPLYHINENEDPLIISFTSGTTGN"
BASE COUNT          831 a          303 c          389 g          714 t
ORIGIN      
        1 attaattcag aaaaaatgga tgtaaaatga agatagaaat tataaataat tttatctttg
       61 tagtgactat aagttatttt taatcattaa tcaaaatttt ttatgaatct cgaaaactta
      121 aaaatagccg taattggtgc aggaaacatg ggaagtgctt tgctacacgg ctggataaaa
      181 agcaaatcaa tatctcctca aaatatcagt ataagtgata tttcaaaaga aaaacttgaa
      241 aaaataacaa cttttgaatc aggtgtcaat gttagtttat caaataaaga agtgataaaa
      301 aaagcaaaaa ttattatttt agcagtaaaa ccttggttga tagaggaaat tgcagaagaa
      361 attaaaaatg aaattaacta taaagaacaa atattaattt ctattgctgc gggaatcaat
      421 tttgaaaagt tacttaaaat ttttgataga aaggcaatgt tatttcgggt ggttcctaat
      481 accgccgccg aagtttctga gagtctttgt gccatttcct cgtgtaatgc agatagtgag
      541 caacaacaat taataatgaa tctttttaat tgcataggta aagctttttt agtgccagaa
      601 aatcaactga atgcttttat gtcgctcact tcgtgcggaa tagcttatgt ttttcgatat
      661 attcgcgcag ccatggaagg cggagtagaa attggaattt ctccagaaat ttgtcagcaa
      721 gctgttgttc aaactttaaa aggtgctgta gctttgcttg aagccaatca aactcatccc
      781 gaaatagaga ttgataaagt gacaacgccg ggcggaatta caattaaagg tcttaatgaa
      841 atggaagcta atggtttttc taatgcagtt attcaaggaa tcaaagcttc ttggttgaaa
      901 gattaaagtc aataataaaa tataatttta tcgcattatg aatgaacaaa ttagacaaat
      961 cgcgttgagg ttacgtggct tacgtgaatc tttggatttg acaatttctg aagcagcaga
     1021 aaaatgtgga gtccctaaaa aagattatga aaaatatgaa tcgggcgaaa cagatatacc
     1081 agttagcttc atttgtaaag tagctgaaac ttttggaata gaaaccactg ctttaatttc
     1141 tggggaagag cctcgtgcac aagttttttt tgtaactcgc aaaggaacag gagccagtat
     1201 tgaaaggaca aaagcttata aatatcaagc tttagcttat ggttttcgaa atgcaaaagt
     1261 agatcctttt gaggtaatgg ttgagcctaa taaccttcca atttatttga atacacataa
     1321 aaatcaggaa tttaattatg ttttggaagg tactttatta ctggaaattg atggaaaaca
     1381 actaacctta tatgaaggag atagtattta tttcgatgct accaaaccac atggaatgaa
     1441 agcgttaaat gataaaaaag tgagattttt agctataatt atttgaaaca atttactgat
     1501 gatattaaaa atcataattt ttgagatgga ataaaatttt tttaaaaaag aaatgctttc
     1561 gtaaataaga aagtgtttct ttttttgtta aaataaaaaa attactatga tgttagaaaa
     1621 atttgtaaaa caaactgaat ttaaagattt tgaagatttt aataaaaatt ttcagcttat
     1681 agttcctgac cacttcaatt ttgcttacga tgtagtagat gaatgggcaa aaactaatcc
     1741 taacaaacaa gctatggtat ggacaaatga tcaacgcgaa tgcagaaaat tcacttttgc
     1801 tgacatgaaa aaatatagcg atcaaacagc ttcatatttt caaagtcttg ggataaaaaa
     1861 aggagatatt gtgatgctta ttttaaaacg acgttatgag ttttggtttt ccattttagc
     1921 ccttcataaa attggtgcaa tagccattcc tgcaactcat ttactaactt ccaaagattt
     1981 aatttatagg aacaatgctg ccgatataaa agctattgta gctgtaggtg aagaaccaat
     2041 tttaacacat atcaacgagt cgttgcctga ttctacaact gtaaaatttt taatttcaat
     2101 tggaccaaaa gttcctgaaa actggcttga ttttcataaa ggcattaaag aagcagctcc
     2161 ttttattcca ctttatcata ttaatgaaaa tgaagaccca ttaattatta gtttcacttc
     2221 aggaaccaca ggaaatc
//
LOCUS       DAOTKS010000281         2210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_39759_length_2210_cov_5.335035, whole genome shotgun sequence.
ACCESSION   DAOTKS010000281 DAOTKS010000000
VERSION     DAOTKS010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39759_length_2210_cov_5.335035"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1130)
                     /locus_tag="P5236_05720"
     CDS             complement(<1..1130)
                     /locus_tag="P5236_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HRU63897.1"
                     /translation="MKKLLLLFFSLFIILSVFSQNIQVQGIVVGQDNEPLPGVNVVVK
                     NTTNGTITNKEGQFTLNVPSNGILVVSYVGYKTQEVPVNGRNFIRIVLEESSEALEEL
                     VVIGYGVQKKSVVTAAISKVTSDELNVVKPSRVEDVLKGKISGVQITQSSGQPGSDSK
                     VRIRGIGTVNNSEPLYIVDGMPVDGGINYLNPVDIQSVEVLKDAASAAIYGARAANGV
                     ILVTTKTGEGVLKGVSKSVINYDFNYGWQNPWKKKSVLDAKEYMVIMNEALINDGNLP
                     RYTASQIQSAGKGTDWQEETFNFDAPVQTHQLSMSGNSEKASYFLSMGYFDQDGIIGG
                     NYGKSNYERWSLRTNATYNVFEDKTRDILNKFRVGVNVGYSR"
     gene            complement(1497..>2210)
                     /locus_tag="P5236_05725"
     CDS             complement(1497..>2210)
                     /locus_tag="P5236_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63898.1"
                     /translation="KNMLPDKVRKKISEIETDFSENKLECVNILKGFKALKLTESFSE
                     FKYLKRSGYSFQLVLSSMIYAVTLGSKTAVSSLPLLKEKGITMGKDVLYRLKNNGGIN
                     WRKILWQIACKFISITKGENEGEHKVRCLIFDDTLLEKTGKKIEKIGKVHDHVTDTFK
                     LGYKLLLGLYWDGKSTIPLDFSFLREKGKRSAKPYGMSRKELHFIIFCLSNLKSKYET
                     LVNLNLLLICFFGCTIFLT"
BASE COUNT          732 a          436 c          271 g          771 t
ORIGIN      
        1 tctcttgaat atcctacatt aactccaact ctaaacttgt taagaatatc acgagttttg
       61 tcttcaaata cattgtaagt agcattggtt cgcaaactcc atctttcata gtttgatttt
      121 ccgtaatttc cacctataat accatcctga tcgaaatatc ccatagaaag aaaatacgat
      181 gctttttctg aatttccact catgctaagt tgatgagttt gtactggcgc atcaaaattg
      241 aaagtttcct cttgccaatc tgtacctttg cctgcacttt gaatctggct tgcagtataa
      301 cgtggtaaat ttccatcatt aatcagtgct tcattcataa ttaccatgta ctctttagca
      361 tcgagtacag atttcttttt ccatggattt tgccaaccat aattaaaatc ataattaata
      421 acagatttag aaaccccttt taaaactcct tcacctgttt tggtggtaac aagaataact
      481 ccatttgcag ctcgagcacc ataaattgct gcagaagcag catctttaag tacttctact
      541 gattgaatat ccacagggtt taaataatta attccaccat ccacaggcat accatcaacg
      601 atataaagag gctcactatt attaactgtc cctatgcctc gaattcgaac tttggaatca
      661 gatcctggct gtcctgaact ttgcgtaatt tgtacacctg agattttacc ctttaataca
      721 tcttctaccc gtgaaggttt tactacattt aattcatcgg aagtaacttt actaatagca
      781 gcagtaacga cacttttttt ctgaacacca taaccaatta caactaattc ttcaagtgcc
      841 tctgaacttt cttctaaaac aatgcgtata aaattacgtc cgtttacagg aacttcctgg
      901 gttttataac ccacataaga taccactaag atgccattgg aaggaacatt gagagtaaat
      961 tgtccttcct tgttagtaat cgtaccattt gtagtatttt ttactacaac atttacacct
     1021 ggcaagggct cattatcttg cccaacaact ataccctgta cctgtatatt ctgtgaaaat
     1081 acagaaagga taataaaaag cgaaaagaac aacaataata attttttcat aaatttttaa
     1141 tgaattttaa gattaatact atgttcttaa tgaacattac aaatataagt ataaaatcaa
     1201 aaaaatgtac ttattttgtt tctacataaa tacgtcatcg gtaaaaaaaa tatacgtttt
     1261 aaatacttca aaatatgcta ttattctgat ataaagaata ttatctttac taaaaaatct
     1321 ttggttttgt cgtaaaggat tttacaattc tatattaatt tactttcgga tttgaatttt
     1381 taaataaaca aggagatatg ttaaataatc tctaatcaga attagaataa taagttctta
     1441 acttgcttca agttgttcat aatatttatc taattgaaaa tcaaataaaa aaatgattaa
     1501 gttaaaaata tagtacaacc gaaaaaacaa attaaaagca gatttaaatt aactaaagtt
     1561 tcgtacttcg atttaagatt tgaaagacag aaaataatga agtgcaattc cttcctactc
     1621 attccatagg gcttcgctga tcgttttcct ttctctctta aaaaagaaaa atccaatgga
     1681 atcgttgact ttccatccca atacaatcct aacaataact tgtatcccaa cttgaaagtg
     1741 tctgttacat gatcgtgtac tttcccaatc ttttctatct ttttacctgt cttctcaagc
     1801 aaggtatcat caaatatgag gcatcgtacc ttatgctctc cttcattctc tcctttcgtt
     1861 atactgataa acttacatgc tatttgccat aatatcttcc gccaattgat ccccccatta
     1921 ttcttcaaac gatacaatac atccttaccc atcgtaattc ctttttcctt taacaggggc
     1981 agagaagata ctgccgtttt actgcccaat gttactgcat aaatcatgga tgacaatacc
     2041 aattgaaaac tgtaaccact ccttttcaaa tacttaaact ctgaaaaact ttctgtcagt
     2101 ttcaacgcct tgaaaccttt caaaatattt acacattcta atttattttc actgaaatct
     2161 gtttcaattt cagaaatctt ttttctaact ttgtctggta gcatattttt
//
LOCUS       DAOTKS010000282         2202 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_39933_length_2202_cov_4.388915, whole genome shotgun sequence.
ACCESSION   DAOTKS010000282 DAOTKS010000000
VERSION     DAOTKS010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39933_length_2202_cov_4.388915"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..349
                     /gene="tnpA"
                     /locus_tag="P5236_05730"
     CDS             92..349
                     /gene="tnpA"
                     /locus_tag="P5236_05730"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HRU63899.1"
                     /translation="MEDEHIYKRYNKTLLLYHIVLPIKYRKKMITESIGNSLKCICME
                     ISERYEINFIEIGYEEDDVHFLLQSVPTYSVSTIVTKLKSS"
     gene            346..546
                     /locus_tag="P5236_05735"
     CDS             346..546
                     /locus_tag="P5236_05735"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRU63900.1"
                     /translation="MTVRELFLRHPEIKKQLWGDGFLTSGYYAKTVGLYGSGDVIMAY
                     VSNQGNEKKYKKLYDGQFALFD"
     gene            594..1418
                     /locus_tag="P5236_05740"
     CDS             594..1418
                     /locus_tag="P5236_05740"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRU63901.1"
                     /translation="MEKITNEFLKRSISAVVYVLLVVASLFISPATFVIVFAFFMSMC
                     IYEFHKIINQQPEVNVPRWLSMGISLLLFLISFFYFSGRAFLPIFSYYIILIFWMFIR
                     ELFLKNPNPVHNWAYFLLGQVWIALPFSLLNFIVYFNNYQPLLILSIFILVWMNDTGA
                     YLIGITLGKHKMIPRVSPHKSWEGFAGGILVTLLTGYLLSLWLYQLDLPQWLFMSLIV
                     SIFATFGDLLESLWKRTFQIKDSGNAIPGHGGFLDRFDSLLMVVPAVLIYLIFLFN"
     gene            1471..>2202
                     /locus_tag="P5236_05745"
     CDS             1471..>2202
                     /locus_tag="P5236_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRU63902.1"
                     /translation="MNPNFDKIRCYNDEEVHDVLERLCNEKQFMKIMSTVYPFLPKEM
                     IKKRLLSIHTQLDFQKEIILPFLQYLVANVTTNGIALQGLEKIIKEESYLYLSNHRDI
                     VSDPMFLCYKFLENGFDTVEVAIGDNLLIYPWIEDLVRLNKSFIVKRGLTTHQMLESS
                     KLLSEYIWYTLHEKKHSIWMAQREGRAKDSNDKTQESLLKMLNLAGKGSFIENMMELN
                     ICPLSISYEYDPCDYLKAKEFQLRRD"
BASE COUNT          685 a          309 c          399 g          809 t
ORIGIN      
        1 attttaatca aaagaattcc tcgaggcttg tgtccgggtt aatcccaaag aaaccatagg
       61 tttctaaaaa taaattgtta tttttgtgtg aatggaagat gaacatattt ataagcggta
      121 taataagaca ttgttacttt atcatatcgt tttgcctata aaatatcgca agaagatgat
      181 tacagaatca ataggaaatt ctctgaagtg tatttgcatg gaaatatcag agcgttacga
      241 aatcaatttt atagaaatag gctatgaaga agatgatgtt cattttttat tgcagagtgt
      301 acccacctat tcagtttcaa caatagtaac aaagttaaag agttcgtgac ggtacgagaa
      361 ctctttttga ggcatcctga aataaagaag cagttgtggg gtgatggttt tttgacaagt
      421 ggctattatg caaagacagt aggattatat ggaagtggag atgtaataat ggcttatgtt
      481 tcaaatcagg gaaatgagaa gaaatataaa aaattgtatg atgggcaatt tgcattgttc
      541 gactaacaag atacctcgag gttatttaat tctgaatttt tataaaatta acaatggaaa
      601 agataacaaa tgaattttta aaacgctcca tcagtgctgt tgtttatgta ctgttggtcg
      661 tagcaagcct ttttatcagc cccgcaactt ttgtaattgt ttttgccttt tttatgtcta
      721 tgtgtattta tgaatttcat aaaattatca atcaacaacc agaagtaaat gtaccacgct
      781 ggctttcaat gggaatcagt cttctgcttt ttttgatttc tttcttttat ttttctggaa
      841 gagctttcct tcctattttt tcttattata ttattctcat cttctggatg tttatcagag
      901 aattattttt gaaaaatccc aatcctgttc ataattgggc ttattttttg ttaggacagg
      961 tttggattgc gttgccattt tcgcttctta attttattgt gtattttaat aattatcaac
     1021 cgcttttaat tctttcaatt tttattcttg tctggatgaa tgatacagga gcttatttaa
     1081 tcggcataac tttggggaaa cacaaaatga ttccacgcgt ttcacctcat aaatcatggg
     1141 aaggttttgc aggaggtatt cttgtaactt tattaacagg ttatttgctt tctttgtggc
     1201 tttaccaact tgatttgcca caatggctat ttatgtcttt aatagttagt atttttgcaa
     1261 cttttggcga tttgctggaa tcactctgga aaagaacgtt tcaaattaaa gatagtggaa
     1321 acgccattcc aggacatgga ggctttttag atcgctttga cagtttgctt atggtggttc
     1381 ctgctgttct tatttatttg atttttttat ttaactaatt acttgttaat aattgaatta
     1441 aataaaattt ttcttaaaat tcagtgaaaa atgaatccta attttgataa aatacgttgt
     1501 tacaatgacg aggaagttca tgatgtttta gaacggttat gtaacgaaaa acagtttatg
     1561 aaaataatga gtactgttta tccgttcctg cctaaggaaa tgataaaaaa acgtctgtta
     1621 agcattcata ctcaattgga ttttcagaaa gagattattt taccttttct tcaatatctt
     1681 gttgccaatg taacaaccaa tggaatagct ttgcaaggac ttgagaaaat catcaaggaa
     1741 gagtcttacc tttatctttc taatcatcgg gatattgttt ccgatccaat gtttctttgt
     1801 tataaatttt tggaaaatgg tttcgatact gttgaagtgg ctattggtga taatttattg
     1861 atttatccct ggattgaaga tttagtgcgt ctgaacaaaa gttttattgt aaaacgtggc
     1921 ttgaccaccc atcagatgct ggaaagttcg aaattacttt ctgaatatat ttggtacaca
     1981 cttcacgaaa aaaaacattc tatctggatg gctcaacgag aaggtcgtgc gaaagactcc
     2041 aatgataaaa cacaggaaag ccttcttaaa atgctgaatt tggcagggaa aggttctttt
     2101 attgaaaata tgatggaact aaatatctgt cctttaagta tttcttatga atatgaccct
     2161 tgtgattatt taaaagcaaa ggaatttcaa ttacgtagag at
//
LOCUS       DAOTKS010000283         2199 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_40004_length_2199_cov_4.813433, whole genome shotgun sequence.
ACCESSION   DAOTKS010000283 DAOTKS010000000
VERSION     DAOTKS010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40004_length_2199_cov_4.813433"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="P5236_05750"
     CDS             <1..239
                     /locus_tag="P5236_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRU63903.1"
                     /translation="NVDKHIEEKTFLYNSLHSKHKVNAGKYHADFIIDEKYFVKCDQK
                     MHSSKNHSKIIYAANGIEIGKKNEIPLWLFGFLY"
     gene            346..>2199
                     /gene="nifJ"
                     /locus_tag="P5236_05755"
     CDS             346..>2199
                     /gene="nifJ"
                     /locus_tag="P5236_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HRU63904.1"
                     /translation="MAKTKKFLTCDGNQAAAHIAYMFSEIAAIYPITPSSPMAEYIDE
                     WTAYGRKNIFNETVQVQEMQSEAGAAGAMHGALQAGALASTFTASQGLLLMIPNMYKM
                     SGELLPGVFHVSARSLATHALSIFGDHQDVMAVRQTGCALFATGSVQEIMDLAPVAHL
                     SAIKSRIPFVHFFDGFRTSHEVQKIEQLDKEDLIPLIDQEALKAFRQRALNPNQPVTR
                     GTAQNPDIYFQATEAANPFYETVPEIVEDYLNKLAEITGRKYHLFDYYGAEDADRVVI
                     AMGSSTEAIREGVDYLMAKGEKVGLISVHLYRPFSAKHFLAVLPKTVKRISVLDRTKE
                     PGANGEPLYLDVKNALYGQKDAPIVVGGRYGLSSKDFTPAQVLAVFENLALPEPKNHF
                     TVGIVDDVTFTSLPLREEISLGGEGIFEAKFYGIGSDGTVGANKNSIKIIGENTNKYC
                     QAYFAYDSKKSGGFTCSHLRFGNHPIRSTYLVTTPDFVACHVQAYLKLYDVTKGLKKN
                     GTFLLNTVWNEEEVKKNLPNKVKRYLAKNNINFYIINATNIAQEIGLGNRTNTILQSA
                     FFKISNVIPYELAVEKMKEMIDKSYGKKGEEIVKKNYAAVDRGTEFIKIEVP"
BASE COUNT          744 a          326 c          390 g          739 t
ORIGIN      
        1 gtaatgttga taaacatata gaagaaaaaa cttttttata taattcttta cattctaagc
       61 ataaagtaaa tgcagggaaa tatcatgcgg attttataat tgatgaaaaa tattttgtta
      121 aatgtgatca gaaaatgcat tcttcaaaaa atcattcaaa aattatttat gctgctaatg
      181 ggattgaaat aggtaaaaaa aacgaaattc ccttatggtt gtttggtttt ctttattgat
      241 caatgagata atctttaatc ataaataaat aaaatttttt aaaataaaat tcatatattt
      301 gtttaatttt caaataatta ataatcattc aataactata ataaaatggc taaaacaaag
      361 aaatttttaa cttgcgatgg taatcaagca gcagctcata tagcttatat gtttagtgaa
      421 atagctgcta tttatcctat tactccttcc tcacctatgg cggaatatat agatgaatgg
      481 acagcctatg gtcgaaaaaa tatttttaat gaaacggtgc aagttcaaga aatgcaatct
      541 gaagcaggtg ctgctggagc tatgcacggg gctcttcaag caggagcatt ggcaagtact
      601 tttacagctt cacagggttt gcttttgatg attcctaaca tgtataaaat gtctggtgag
      661 ttattgccgg gtgtttttca tgtgtcagca cgatctcttg ctactcatgc tctttctatt
      721 tttggcgatc atcaggatgt aatggcagtt cgtcaaacgg gttgtgctct ttttgcaact
      781 ggttcagttc aagaaattat ggatttagct cctgttgcac atttatcagc aataaaatct
      841 cgtattcctt ttgtacattt ttttgatggt ttccgtactt cccacgaagt tcaaaaaata
      901 gaacaattgg ataaagaaga tctaattcct ttaattgatc aagaagcttt gaaggctttt
      961 agacaaagag ctttaaatcc taatcaaccg gtaactcgag gtacagctca aaatcctgat
     1021 atttattttc aagctaccga agctgctaat cctttttacg aaacagtacc tgaaattgta
     1081 gaagattatc ttaataaatt agctgaaata acaggaagaa aatatcatct tttcgattat
     1141 tatggtgcag aagatgctga tcgtgttgta attgcaatgg gttcttcaac cgaagctatt
     1201 cgagaaggag ttgactattt gatggctaag ggtgaaaaag ttggtttgat ttctgtgcat
     1261 ctttatcgtc ctttttctgc aaaacatttt ttagctgttc ttcctaaaac tgtaaaaaga
     1321 atcagtgttt tagatcgtac aaaagaaccc ggtgcaaatg gtgaaccttt atatctggac
     1381 gtaaagaatg ccttatatgg acaaaaagat gctccaatcg tggtaggtgg acgctatggt
     1441 ttgtcttcta aagattttac tcctgctcaa gtattagctg tttttgaaaa tttagcttta
     1501 ccagagccca aaaatcattt tacagtagga attgttgacg atgttacttt cacttctctt
     1561 ccactaagag aagaaatttc actgggtggt gaaggaattt ttgaagctaa attttatggt
     1621 atcggttctg atggaacagt aggagccaat aaaaattcaa ttaaaatcat tggggaaaat
     1681 actaataaat attgtcaagc ttattttgcc tatgactcaa aaaaatcggg aggatttacc
     1741 tgttctcatc ttcgttttgg caatcatcct atacgttcaa cttatttagt taccacacca
     1801 gattttgttg cttgtcatgt tcaagcttat ttaaagcttt atgatgtaac taaagggctg
     1861 aaaaaaaatg gaactttcct tctgaatacg gtatggaatg aagaagaagt aaagaaaaac
     1921 ttgcctaata aagtaaaaag atatttagca aaaaataaca ttaattttta tattattaat
     1981 gccacaaaca ttgctcagga aattggttta ggaaatcgca ccaacactat tcttcaatca
     2041 gctttcttta aaatttctaa tgttattcct tatgaattgg ctgttgaaaa aatgaaagaa
     2101 atgattgata agtcctatgg caaaaagggt gaagaaattg taaagaaaaa ttatgcagcc
     2161 gttgatcgag gaacagaatt cattaaaata gaagttcca
//
LOCUS       DAOTKS010000284         2188 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_40271_length_2188_cov_3.578059, whole genome shotgun sequence.
ACCESSION   DAOTKS010000284 DAOTKS010000000
VERSION     DAOTKS010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40271_length_2188_cov_3.578059"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..286
                     /locus_tag="P5236_05760"
     tRNA            213..286
                     /locus_tag="P5236_05760"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:247..249,aa:Arg,seq:ccg)
     gene            complement(372..2099)
                     /locus_tag="P5236_05765"
     CDS             complement(372..2099)
                     /locus_tag="P5236_05765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRU63905.1"
                     /translation="MKLSKKLFVYFFSLFFLMLLCATYFQYQREKSFKIEQLNHQLSI
                     YNQMVHHYIQKKSLTWNGLNQFIEIFPDSTLRITVIDTLGNVLYDSSLREGTVLENHL
                     HRPEIIMARKSGFGEAMRHSHSTGKDYYYLAQRFPQYYIRCALSYSVGLFTALKANTT
                     FLYFMGFLFLMGTIALYFIARNFSRSINRLRDFTQKVEKNELLDTEITFPNDELGEIG
                     NNFVQIYKSMLESKEEASRERDKLFKHLQIFQEGLGIFSAERKEILTNTHFIQYANLL
                     SDEPCATSEQIFNIPEFKEISEFIDENLAKNQVNRKRFIIEKGGRIFEVQCIVFQDDT
                     FEIFINDISTQEKESNLKHQLTQNISHELKTPVSSILGYMESIIDNPNLNPQRQKFFI
                     ERTYQQAQRLSALLQDISMLSKLDEADQLFEKEECSLSAIIDDVLNDVHLQIEQKNCK
                     VLKNYHEGLTINGNSSLLYSIFRNLMDNALAYGSENITIDINCYREDDQFYYFSFSDN
                     GKGVEEKHLSRLFERFYRVDKGRSRKAGGTGLGLSIVKNAILFHNGNISAKNLPTGGL
                     SFIFTLGKY"
BASE COUNT          711 a          396 c          347 g          734 t
ORIGIN      
        1 ggtgttacgg ataaacaaac tttacttcaa caaattcaag ctgccattta aacccaaatt
       61 tgttagtaaa tagaaaattt accttaaaaa attctcactg aatataaagg aattgtaata
      121 ttgggaaaat atgcacaagt tattcctata ttttatttat gatagcaact tagtataaat
      181 cgagaaaatt aattaccttt gcgtttacaa aaggctccgt agttcagttg tatagaacgt
      241 cagattccgg ttctgaaggt cttgggttag aatcccaacg gggtcacaca cagccaacct
      301 caacattttt ttttgggttg gctttttgat tgctcccaat ttatttctac atcttctaat
      361 aactttttag tttaatattt tcccaaagta aaaatgaaac ttaacccacc agttggtaaa
      421 tttttagcag aaatattccc attatgaaac aaaatagcat tttttacaat ggagagtcct
      481 agacctgtac cgccagcttt gcggcttcgt cctttgtcaa ccctataaaa acgttcaaac
      541 aaacgactca gatgtttttc ttcaacgcct ttgccattgt ctgaaaacga aaaataatag
      601 aactggtcgt cttcacgata acaattgatg tcgatcgtga tattttcgct gccataagcc
      661 agtgcattgt ccattaaatt gcgaaaaatt gaataaagca acgagctgtt tccattgatt
      721 gtcagacctt catgatagtt tttcaaaacc ttgcaatttt tttgctcaat ctgcaaatga
      781 acgtcattca ggacatcatc aatgatagca gaaagtgaac attcttcttt ttcaaacagc
      841 tggtcagctt cgtccagttt gcttagcata gaaatatctt gaagcaaagc actcaatcgt
      901 tgagcttgct gataagttcg ttcgatgaaa aatttttggc gttgaggatt taaattggga
      961 ttgtcaataa tgctttccat gtagcccaaa atactgctta cgggcgtttt taattcgtga
     1021 gaaatgttct gtgtcaattg atgctttaaa ttactttcct tctcctgcgt agaaatatca
     1081 ttaatgaata tttcaaaagt atcatcttga aaaacaatgc attgaacttc aaaaattcgt
     1141 ccaccttttt caatgatgaa tcgtttacga ttcacctgat tttttgctaa attttcatca
     1201 ataaattcac ttatttcttt aaattcaggt atattgaaaa tttgttctga agtcgcacaa
     1261 ggttcgtcag ataataaatt ggcatattga atgaaatgtg tgttggttaa aatttctttt
     1321 ctttcagcag aaaaaatccc caatccttcc tgaaaaattt gtaaatgttt aaataattta
     1381 tcgcgttctc tgctggcttc ttctttcgat tccagcatac ttttgtagat ttgaacaaaa
     1441 ttattgccga tttcgcccag ctcatcattc gggaaagtaa tctctgtatc cagcaactca
     1501 tttttttcaa ctttttgagt aaaatcacgc aaccgattta tcgatctgga gaaatttctg
     1561 gcaataaaat aaagtgctat tgtccccatc aaaaacaaga agcccatgaa atataaaaaa
     1621 gttgtgttgg ctttcagtgc tgtaaaaagg ccaacactat aagacaacgc acaacggata
     1681 tagtattgtg gaaagcgttg agctaaataa taataatctt tgccagtgga atgcgagtgt
     1741 ctcattgcct cgccaaaacc tgatttgcgt gccattataa tttccgggcg atgcaaatgg
     1801 ttttccaaca ctgttccttc acgaagggac gaatcataca aaacatttcc caaagtatca
     1861 atgaccgtaa ttcgcaaagt actgtctgga aaaatttcaa taaattgatt caaaccattc
     1921 caggtgagac tctttttttg aatgtaatga tgtaccatct gattataaat ggataattga
     1981 tgattcaact gctcaatttt gaaacttttt tcccgttgat attgaaaata agtagcacac
     2041 aacaacatca ggaaaaatag gctgaaaaaa taaacaaaaa gttttttact taatttcatt
     2101 gattatgtcc atttgaggaa ttttcttttt ggatatttaa agaatggcag ttttcacaat
     2161 tattcttcaa aacaatagcc ataaccca
//
LOCUS       DAOTKS010000285         2177 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_40549_length_2177_cov_3.780396, whole genome shotgun sequence.
ACCESSION   DAOTKS010000285 DAOTKS010000000
VERSION     DAOTKS010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40549_length_2177_cov_3.780396"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="P5236_05770"
     CDS             complement(<1..1169)
                     /locus_tag="P5236_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRU63906.1"
                     /translation="MKKTSLLSFLLLLIPFIIFAQNQTSSRTFRISKNLNIYNSIFRE
                     LDMFYVDTLNYDKIVRTSIDKMLDNLDPYTVYIPEEETEDLTFMTTGEYAGIGALIMK
                     KGDEVVVSEVYEGMPAQRNDIRAGDVILEVDGQKTTGMSVDKVSALLRGFPNTQIKIK
                     IKRYGEKKIIEKKFIREKIQIKPIVYAGQVAEKIGYIQLGEFTDGVAFELKSSLNELV
                     KNYHITSLVLDIRSNGGGLVDEAVKILGYFLPKGTEVVTTRGKNKELDRIYKTPTEPI
                     YPDMKLAILVNRSSASASEILAGAIQDLDRGVIIGERTFGKGLVQNIRAINFGGHLKV
                     TTAKYYIPSGRCIQAIDYNHRNEDGSVGYVPDSLTSEFSTRNGRKVRDGGGIVPD"
     gene            complement(1192..1644)
                     /gene="coaD"
                     /locus_tag="P5236_05775"
     CDS             complement(1192..1644)
                     /gene="coaD"
                     /locus_tag="P5236_05775"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRU63907.1"
                     /translation="MKRAIFPGTFDPFTIGHYSIVQRGLKLFDEIVIAIGTNQAKKTF
                     FSLEKRKEIINQAFVNEPRVKIMIYDSLTVDFARSIQANFILRGLRSVTDFEYEHSIA
                     DANRKIAGIETVILFTEYEYSYISSTVVRDLISYGKDISAFLPPNVQL"
     gene            1933..>2177
                     /locus_tag="P5236_05780"
     CDS             1933..>2177
                     /locus_tag="P5236_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63908.1"
                     /translation="MKKFVYSAIFLLCGIFAYGQIDFGFKLGYNSSVGLNDLNSITKG
                     KYTLDSAKIDAKNNFMVGAFSRINFGKIYLQPELLYVG"
BASE COUNT          735 a          360 c          314 g          768 t
ORIGIN      
        1 atatcaggta caatgcctcc tccatcgcgt acttttcgac catttcgagt agaaaattct
       61 gaagtcaaac tatcgggaac ataacccaca ctgccatctt cgtttcgatg attgtaatct
      121 atggcttgaa tacatctacc actgggaata taatattttg cagtagttac ttttaaatgt
      181 cctccaaaat tgatagctcg tatattttga accagtcctt ttccgaaagt acgttcgcca
      241 ataataactc ctctgtccag atcttgaatt gcaccagcca aaatttcaga agctgatgct
      301 gaagaacgat taaccaaaat agccagtttc atgtcaggat aaataggctc agtaggtgtt
      361 ttgtaaattc gatccaattc cttgtttttc cctcttgttg ttactacttc tgttcctttg
      421 ggaagaaaat atcctaaaat tttaacagct tcatcaacca gacctcctcc attgctacgg
      481 atatcaagga ctaaagaagt gatatgataa tttttcacaa gctcatttaa agatgatttt
      541 aattcaaaag caacgccatc tgtaaattct cccaactgaa tataaccaat cttttctgcc
      601 acttgccctg cataaacaat aggtttaatt tgaatttttt cacgaataaa ttttttttcg
      661 atgatctttt tttcaccgta acgctttatt ttgattttta tttgagtatt tgggaagcca
      721 cgcaataaag cactaacttt atctacactc attcctgttg ttttttgccc atcaacttcc
      781 aaaataacat ctcccgctct aatatcgttt cgttgagctg gcataccttc atatacttcc
      841 gaaaccacta cttcgtcacc ttttttcata attaaagcac caatacctgc atattcacca
      901 gtagtcataa aagttaaatc ttctgtttct tcctcaggaa tataaaccgt ataaggatct
      961 agattatcta acattttatc aatagaagtt cttactattt tatcataatt aagtgtgtca
     1021 acataaaaca tatccaattc tctgaaaata gaattataaa tattgaggtt tttactaata
     1081 cgaaaagttc ggctactggt ttgattttga gcaaaaatga tgaaaggtat tagtaacagc
     1141 aaaaatgata aaagactggt ttttttcata aagtcagaat aaaaatattt attaaagttg
     1201 aacattggga ggtaaaaatg ctgaaatatc ttttccataa gaaatcaagt cacgaactac
     1261 cgtagaggaa atgtatgaat attcatattc ggtgaataaa ataactgttt caataccagc
     1321 aatttttcgg ttagcatcag ctatactgtg ttcgtattca aaatcagtca ccgatctcag
     1381 ccctcgaagg ataaagttag cttggatgga gcgagcaaaa tccaccgtta aactgtcgta
     1441 aatcatgatt tttactcttg gctcatttac gaatgcttga ttgataattt cttttctttt
     1501 ttcgagagaa aaaaaagttt tttttgcctg attggttcca atggcaatta ctatttcatc
     1561 aaacaacttc aaaccacgtt gcacaatact gtaatgacca attgtaaaag ggtcaaaagt
     1621 ccctgggaaa atagcacgtt tcataaatac agtattaacg ttgaaattaa tatttgaaat
     1681 taaagtactt aataaaaaat ttgaattaca aagataaaaa atttgctctc agtaatacca
     1741 aatcactaag ataagtttat attttttaat ttatgaagta tttttttcat ataaaaagta
     1801 ttttttattt tgcattattg atagaaattc agttgaaaaa ttaaaaaaat gaaaatattt
     1861 tgacaaagaa ggatattttt taagataatt ttctttactt tgaaattagt ttaattttaa
     1921 aagtaaaata ttatgaaaaa gttcgtttat tcggcaattt ttctactatg tggtattttt
     1981 gcctatggtc aaatagattt tggctttaag ttaggatata attcttccgt tggattgaat
     2041 gatttgaatt ccattactaa aggaaaatat acattggatt ctgcaaaaat tgatgcaaaa
     2101 aataacttta tggtaggtgc tttttctcgc ataaattttg ggaaaatata tttacaacct
     2161 gaattattat atgtagg
//
LOCUS       DAOTKS010000286         2159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_41009_length_2159_cov_3.624525, whole genome shotgun sequence.
ACCESSION   DAOTKS010000286 DAOTKS010000000
VERSION     DAOTKS010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41009_length_2159_cov_3.624525"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..1057
                     /locus_tag="P5236_05785"
     CDS             95..1057
                     /locus_tag="P5236_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HRU63909.1"
                     /translation="MNPILYRLYNSFLLALPKAFKTIIWLLKIILPISFVVSLLQYWG
                     IIAIVASFLAPVFSIVGLPGEAAIVFITSIFMALYAPIAIIATLPLGIREITILAIMC
                     LISHNLIVETTVQKKTGSSPWIIFFLRIIASFVAAFLLNKILPDNMGKAQIVEKAIIF
                     KSFGEMFQHWLINTGWLILKIVLIITGLMILQNILKEFHILEYLSKIFAPLMKIMGLS
                     QNTTFLWFVAQIVGLTYGSAIMIEEVTNNSITVEDANLLNYHIAVNHSLLEDTLLFVA
                     IGVPAGWIIVPRFILAILIVWSFRGIHSFESYLKQKQAKFADFS"
     gene            1292..1876
                     /locus_tag="P5236_05790"
     CDS             1292..1876
                     /locus_tag="P5236_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRU63910.1"
                     /translation="MPQKVSKTKKLLIEVARQLFSEKGKKNVTMNDIAEASKKGRRTL
                     YIYFKSKDEIYKAVIDNELDYIFEQIAVVSTENIPPDSKLTKHIITHLDAIKKSVIRN
                     GSLKADFFRDIYEVERTRRKIDMKEIALIKAIFVEGIEKNCFKKMNVELSSIIILYAL
                     KGLEVPYIRQNISNEFEKNKYSIVEFVLTGIKKK"
BASE COUNT          712 a          304 c          341 g          802 t
ORIGIN      
        1 atgtttttca tggtattaga tttaaggtta attaaaaaag attgggttgt cgtgagacaa
       61 ccccttcttg ttttatagaa caagtacttt tctaatgaat cctattttat atcgcttata
      121 taattccttt ctcttagccc ttcctaaagc atttaagacc ataatttggc tattaaaaat
      181 tattttacct atcagttttg ttgtgagttt acttcaatat tggggaatta tagccattgt
      241 tgcttctttt ttagctcctg ttttttcaat tgttggttta ccgggagaag ctgctatcgt
      301 tttcattacc agtattttta tggctcttta tgctcctatt gccattattg caactttgcc
      361 tttgggaata cgtgaaatta ctattcttgc tattatgtgc ctcatttcgc ataacttgat
      421 tgtcgaaact actgttcaga agaaaacagg ctcttctcca tggatcattt tttttcttcg
      481 aatcattgct tcgtttgtgg cagcttttct tttgaataaa attttgcctg ataatatggg
      541 caaagctcaa atcgtagaaa aagcaattat atttaaaagt tttggagaaa tgtttcaaca
      601 ttggttgatt aatacaggct ggcttatttt aaaaatagtt ctaattatta caggactgat
      661 gattttgcaa aatatcttga aagaatttca tattctggaa tatctatcaa aaattttcgc
      721 tcctctaatg aaaattatgg gtctttctca aaacactact tttttgtggt ttgttgctca
      781 aattgtagga cttacttatg gttcagccat catgattgaa gaagtaacaa ataactctat
      841 tactgtagag gatgccaatt tattgaacta tcatattgca gtaaaccatt ctttgctcga
      901 agacacgtta ttgtttgtag caattggtgt gcctgctggt tggattatcg tacctcgatt
      961 tattttagct attcttattg tttggagttt tcgaggtatt catagctttg aaagttatct
     1021 taaacaaaaa caggcaaaat ttgctgattt ctcttaaaag ggacattaaa tttcctcttg
     1081 tcttattttt tgccatactt gtacaaattt gcaatttgtc ctatcaattt ttgttctcgg
     1141 tgcgaatatt atctttctta ttatagatca cagaaatttt cgtcacaagg attattttat
     1201 ctttctgaat aaaagttagg aaaatatttt tttcaaagta acaagtgaag ttttattttt
     1261 taactttgct tattctatca agaaataaat tatgcctcaa aaagtttcta aaacaaaaaa
     1321 attattgatc gaagtggcaa ggcaactttt ttccgaaaaa gggaaaaaga atgtcacgat
     1381 gaatgatatt gccgaagctt ctaaaaaagg aagacgaact ttatatattt attttaagag
     1441 caaggatgaa atatacaaag cagttattga caatgaatta gactatattt ttgagcaaat
     1501 tgctgtagtc tctacagaaa atattcctcc tgatagtaag cttaccaaac atattattac
     1561 tcatcttgat gccattaaaa aatcggtaat tcgaaatggc tcgctaaaag ctgatttttt
     1621 tagagatatt tatgaggtag aacgtacgcg aagaaaaatt gacatgaagg aaattgcttt
     1681 aattaaggca atttttgtag aaggaataga aaaaaattgt tttaaaaaaa tgaatgtaga
     1741 actctcctct attattattc tttatgcttt aaaaggatta gaagttcctt atatccgaca
     1801 aaatatcagc aatgaatttg aaaaaaataa atatagtatc gttgaatttg tactaactgg
     1861 cataaagaaa aaataaccca atcatacagc ttttttattt tatctttggc tatcaattta
     1921 ttacctttcg aaaagtgtaa ttaatcattc aaaatcagtt atagattatt gagtgctcaa
     1981 tgaaagttag aattcaacac tgcttgaaaa gacaagagtt gttattcatc taaacagacg
     2041 acaatcattt ctgaaaatta aagttttgtt ataattttgc agttgaaaag aagaggtaat
     2101 ttgtaaaaaa caagaagatg gctaataatt ctttattgga aaaactggaa ggactacaa
//
LOCUS       DAOTKS010000287         2140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_41485_length_2140_cov_4.895923, whole genome shotgun sequence.
ACCESSION   DAOTKS010000287 DAOTKS010000000
VERSION     DAOTKS010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41485_length_2140_cov_4.895923"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..290
                     /locus_tag="P5236_05795"
     CDS             72..290
                     /locus_tag="P5236_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HRU63911.1"
                     /translation="MSKRLTRSNKNKLLAGVCGGIAKFLGWGPTIVRILYVLLSILSA
                     AFPGILVYLILWLVMPLEKETEEKRNNR"
     gene            409..>2140
                     /gene="uvrB"
                     /locus_tag="P5236_05800"
     CDS             409..>2140
                     /gene="uvrB"
                     /locus_tag="P5236_05800"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HRU63912.1"
                     /translation="MLFKLESPYRPTGDQPEAIQQLVEGIKANVPYQTLLGVTGSGKT
                     FTIANVIEQVQKPTLVLSHNKTLAAQLYSEFKSFFPHNAVEYFVSYYDYYQPEAYLPL
                     TDTYIEKDLSINQEIDKLRLSAISSLLSGRRDVIVVSSVSCLYGIGNPEDFTNNVIEI
                     HKGQHLVRNVFLQNLVNSLYSRNEIDLNRGNFRVKGDTVDVFLAYADYLLRVEFWGDE
                     IDEILTVDPVDFDVIDSFESFKIYPASIFVTTRERINTAIHQIQEDLTLQVEYFRSVG
                     KDLEAKRLYERVSYDIEMIKELGYCSGIENYSRYFDGRAPGTRPFCLLDYFPKDFLLV
                     IDESHVTVPQIRAMYGGDASRKQNLVEYGFRLPAAKDNRPLKFEEFEALIPQTIYVSA
                     TPDDYELMKSNGIVVEQVVRPTGLLDPIIEVRPSLNQIDDLMEEIASRVEKNERILVT
                     TLTKRMAEELTDYLLKMDIRCNYIHSDVETLDRVQIMEDLRKGVYDVLIGVNLLREGL
                     DLPEVSLVAILDADKEGFLRSFRSLTQTSGRAARNINGKVIMYADTITESMKRTIEET
                     NRRREKQLKYN"
BASE COUNT          658 a          338 c          413 g          731 t
ORIGIN      
        1 tcatttttat ctgcgtaatt ttgatttttt ttgtcattta ttttgttact ttgcagaaaa
       61 actgaatttt tatgagcaaa agattaaccc gctcaaacaa aaataagtta ctcgctggag
      121 tttgtggagg tatagctaaa tttttagggt ggggtccaac tattgttaga attctttatg
      181 tgcttctttc cattcttagt gcagcctttc ctggtatatt ggtttatctc attctttggt
      241 tggtgatgcc cctcgaaaaa gagacggaag aaaaacggaa taatcgttga actttttctt
      301 gatatgtatg ttttttgata attatttcgg aagaataatt taaaaacatg agttcttttc
      361 aaagataatt ttataacttt tcatttttca ataaactttc ttttctttat gctttttaaa
      421 cttgaatcgc cttatcgtcc cactggtgac caaccggaag ctattcaaca actggttgaa
      481 ggaataaagg caaatgttcc ttaccaaact ttattgggcg taacaggttc aggaaaaact
      541 tttactattg ctaacgtcat tgaacaggta caaaaaccta cgctggtatt aagtcataat
      601 aaaactttgg ctgcacaact ttacagtgaa ttcaaatcat tctttcctca taatgcagta
      661 gaatattttg tttcctatta cgattattat caaccagaag cttatttacc tcttactgac
      721 acctatattg aaaaagattt atccatcaat caggagattg ataaactacg tttatcagct
      781 atctcatctc tcctttcagg acggcgagat gtgattgtag tttcttctgt atcctgtctg
      841 tatggaattg gaaatccaga agatttcacc aataatgtca ttgaaattca caagggtcag
      901 catttggtca gaaatgtttt tttacaaaat ttagtaaaca gtctttacag ccgaaatgaa
      961 attgatttaa atcgtggcaa ctttcgtgta aaaggtgata cagtagatgt ttttttagct
     1021 tatgctgatt atttgcttcg agttgagttc tggggtgatg aaattgacga aattttaacg
     1081 gtggatcctg tcgattttga cgtaattgat tcatttgaaa gttttaaaat ttatccagcc
     1141 agtatttttg ttaccaccag agaaagaatc aatactgcca ttcatcaaat tcaagaagat
     1201 ttgacacttc aggtggaata tttccgctcc gttggaaaag atttggaagc aaaacggctt
     1261 tacgaaaggg taagttacga cattgaaatg atcaaagaat taggttattg ttctggtatc
     1321 gaaaattatt cacgttattt tgatggacga gcaccgggca ctcgaccttt ctgtttgctt
     1381 gattattttc ccaaagattt tttactcgta attgatgaaa gtcatgtaac ggttccacaa
     1441 attcgtgcaa tgtatggtgg tgatgcttcc cgaaaacaaa atttggtaga atatggtttt
     1501 cgtttgccgg ctgccaaaga taatcgtcct ttgaaatttg aagaatttga agcacttatt
     1561 cctcaaacaa tttatgtaag tgctacgcct gatgattatg agttgatgaa atcaaatggt
     1621 attgtagtag aacaggtggt acgtcctaca ggattgctgg atcccattat cgaagttcga
     1681 ccgagtttaa atcagattga tgatttaatg gaggaaattg cttcaagagt ggaaaaaaat
     1741 gaacgaattc tggtaaccac tcttaccaaa cgaatggcag aagagctaac agattatctt
     1801 ctaaaaatgg atattcgttg caattatatt cattctgatg ttgagacact ggatcgtgta
     1861 caaattatgg aagatttacg aaaaggtgtg tatgatgtgc ttatcggtgt aaatctgttg
     1921 cgtgaaggtt tggatttgcc tgaggtttcg ctggtagcca ttcttgatgc agacaaagaa
     1981 ggttttttgc gttcttttcg ttcattgact caaacctctg gaagagcagc tcgcaatata
     2041 aatgggaaag ttattatgta tgctgatact attacagaaa gcatgaaaag aaccattgaa
     2101 gaaaccaacc gacgccgaga aaaacaattg aaatataatg
//
LOCUS       DAOTKS010000288         2135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_41615_length_2135_cov_3.182212, whole genome shotgun sequence.
ACCESSION   DAOTKS010000288 DAOTKS010000000
VERSION     DAOTKS010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41615_length_2135_cov_3.182212"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="P5236_05805"
     CDS             <1..940
                     /locus_tag="P5236_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRU63913.1"
                     /translation="FDMGVKSGQHPLNILFNDTSLYILDCGKQFTWINDVDGVLGDGR
                     ILVMSKDGSKVETMLTNKGAAFDDPYYGYIEGNTLYFSDRNTGIRTIKLNERNKSLTP
                     ADFPYYVQNNTLGYYNKGGLSFGAINACFGKINNVWYWCKTHNGYGIFRFTDADILKT
                     PTSGEAPAPAAGVVLEGMRPKSFVWDANRDYFYFSLFEPAGSGGLYRCKLAELDSIKL
                     ANLANYKCTLQNGKMVAPIIEAGKGEGSSGEFIGICQLALDETDGCVYFGYRSSDPTI
                     KSGLMRYNPAKGYIEPVIEGVEIYGVAINKTPSKLF"
     gene            1510..>2135
                     /locus_tag="P5236_05810"
     CDS             1510..>2135
                     /locus_tag="P5236_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HRU63914.1"
                     /translation="MKKNIYIFFLIVALLNVEKMFAQSPKREMRAAWLATVWRLDWPS
                     VTVPKATGTNEAARQAAIQQQKNELIAIFNKLQGANMNAVFFQVRGMCDAMYQSSYEP
                     WSAYISSERGADPGYDPLAFAIEEAHKRGLELHAWLNPYRYSSSIYTHGETPMDYYIT
                     HPDWLLDYGGYTKILNPGLPEVRQRIKCIIGEIVNNYHVDGIVFDDYF"
BASE COUNT          736 a          318 c          395 g          686 t
ORIGIN      
        1 attcgatatg ggagtaaaat cagggcaaca tcccttaaat attttgttca atgatacttc
       61 tctttatatt ttggactgtg gcaaacaatt tacttggatc aacgacgtag atggtgtgct
      121 gggcgatgga agaattttgg ttatgtctaa agatggcagt aaagtggaaa ccatgttaac
      181 caataaagga gctgcttttg atgaccctta ttatggttac atagaaggta atacacttta
      241 tttttcagat cgaaatactg gtattcgaac cattaaatta aacgagcgta ataaatctct
      301 tactcctgct gactttcctt attatgtgca aaataacacc ttaggatatt ataataaagg
      361 aggattaagt tttggtgcta taaatgcatg ttttggtaaa attaataatg tttggtactg
      421 gtgtaaaaca cataatggat atggtatatt cagattcact gatgccgaca ttttaaaaac
      481 ccctacaagt ggtgaagctc ctgctcctgc agcaggcgtt gtcttagaag gaatgcgtcc
      541 taaatctttt gtctgggatg ctaatcgaga ttatttctac ttttcccttt ttgagcctgc
      601 aggttctggc gggctttatc gctgtaaact tgctgaatta gatagcatta agttagcaaa
      661 tttagccaat tataaatgta cgcttcaaaa tggaaaaatg gtagcaccga ttatagaagc
      721 cggaaaaggt gaaggttcat caggtgaatt cattggtatt tgccaattag cattggacga
      781 aacagatggt tgtgtttatt ttggttaccg ttcaagtgat ccaactatca aatcgggctt
      841 gatgcgttac aatcccgcta aaggatatat tgaacctgtg atagaaggag ttgaaatata
      901 tggagtagcc attaataaaa caccatcgaa attattctaa aatacaaata aattcttatg
      961 aagttcaata gaagtggaat atcttatttg ttaataatga aaatattttc atagagaatt
     1021 tacaagaaag tggattttta cttaaaaagt ttgacaaaaa aatatcacat tttaaaatct
     1081 atcttgttaa tcgtagagga ataaaattca ttaatttgaa gttggaaaag agttgagtcc
     1141 attattaaat gaatatgaaa taaattaata aattgaacct tctcaggcta aaaaccttaa
     1201 taacttataa caaacaaatt agtaaaacct tattacctta ttagaattaa aaaaacaaaa
     1261 taaatgtaga ttaaaaagga acaataatcc acttaaaata aggtaataag gttgttaacg
     1321 aatgagaaat tttagtgtta tgaaggttat agattttgat tttaataagg tttaaaagac
     1381 aaaataatga aactgatttc aaatagataa aaaaaattca tgtactttga gtacgaggat
     1441 gaaattttga acttcttatt catttccata aaatgctgat attgcttaac ttaatataat
     1501 tattttcgga tgaaaaaaaa catttatatt tttttcttga tagttgcttt actgaatgta
     1561 gaaaaaatgt ttgcccagtc acccaaacgt gaaatgcgtg ccgcctggct ggcaactgta
     1621 tggagacttg attggccgag tgttacagta ccaaaagcta caggcaccaa cgaagcagct
     1681 cgccaggcag ccattcaaca acaaaaaaac gagttaatcg ctatttttaa taaattgcaa
     1741 ggagctaata tgaatgccgt cttttttcaa gtaaggggca tgtgcgacgc catgtatcag
     1801 tcttcctatg aaccctggag tgcatatatt tcaagtgaac gaggagctga tccaggttac
     1861 gatccactgg cttttgccat tgaagaagct cacaaacgag ggctcgaatt acacgcctgg
     1921 ctcaaccctt atagatattc ttcttctatt tatactcatg gtgaaactcc gatggattat
     1981 tacattaccc atcctgactg gttgttagat tatggtggtt ataccaaaat tttaaatcca
     2041 ggattgcccg aagtacgcca aagaattaaa tgtattattg gagaaattgt taataattac
     2101 catgtcgatg gaatagtttt tgatgattat tttta
//
LOCUS       DAOTKS010000289         2133 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_41673_length_2133_cov_5.239654, whole genome shotgun sequence.
ACCESSION   DAOTKS010000289 DAOTKS010000000
VERSION     DAOTKS010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41673_length_2133_cov_5.239654"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="P5236_05815"
     CDS             complement(<1..689)
                     /locus_tag="P5236_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HRU63915.1"
                     /translation="MKLAIMQPYFLPYIGYFQLIKAVDTFIIYDDVQFIKGGWINRNY
                     LLLNDEKFMFNLILKGASANKLINEISVQSNQNKLLKTIYYSYNKAPYFESVFPIIKN
                     ILNYEDKNLSKFIGNSIIKICNYLKIDTEIHYSSALEKDNELKAQDKIIHICKLLNAD
                     TYINAIGGQKLYDKKVFNDSHIDLKFIKSNLTPYTQFKNEFIPCLSILDVMMFNSVDE
                     INIILKNHELI"
     gene            complement(691..1803)
                     /locus_tag="P5236_05820"
     CDS             complement(691..1803)
                     /locus_tag="P5236_05820"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRU63916.1"
                     /translation="MNRQITVTSPFLPPLEEFIPYLEKIWESKWLTNNGQFHQELEKT
                     LCEYLHVDYISLFTNGTLPLMVALQALRITGEVITTPFSFVATTHSLWWNGIKPVFVD
                     IDPKTGNLDPEKIEAAITPKTTAIMPVHVYGNPCDTKRIEEIADTYGLKVIYDAAHSF
                     GVQVNGESILNAGDLATLSFHATKTYNTFEGGALVCHDAKTKQRIDYLKNFGFAGETT
                     VVAPGINGKMDEVRAAFGLLQLKYVDDAIAKRKAIAQQYREQLREIKGITFLKDFPGV
                     KHNYSYFPIFVDENEYGMSRDELYEKLKANNIYGRRYFYPLISDFSTYRGLPSAAKEN
                     LPNAHKIANEVICLPIYPDLEMSDVTIICNCIKKSL"
BASE COUNT          679 a          348 c          348 g          758 t
ORIGIN      
        1 taaattaatt cgtgattctt aagtattata tttatctcgt ccactgagtt gaacatcata
       61 acatccaata tagaaagaca gggtataaat tcatttttaa attgagtgta gggagttaaa
      121 ttacttttta taaattttaa atcaatatgg gaatcattaa atactttttt atcatataat
      181 ttttgcccac caatagcatt tatataagta tcagcattta aaagtttaca tatatgaata
      241 attttatcct gtgcttttaa ttcattatcc ttttctagtg ctgaagaata gtgaatttct
      301 gtatcaattt ttaaatagtt acatatttta atgatactgt ttccgataaa ttttgacaag
      361 tttttatctt catagtttaa aatgtttttt ataataggaa aaacagattc aaaatacgga
      421 gctttattgt atgaataata tatggttttt aataatttgt tttggttcga ttgaacggat
      481 atttcgttta taagtttatt ggcgcttgct ccttttaaaa tcaaattaaa cataaatttt
      541 tcatcattca ataacaaata gtttctattt atccatccac cttttataaa ttgtacatca
      601 tcataaatta taaaggtatc cacagcttta atgagttgga agtaacctat gtaaggtaaa
      661 aaataaggct gcatgattgc aagtttcatt tcacaaggat ttttttatgc agttgcaaat
      721 tattgtaaca tcactcattt caagatcagg ataaatcggc aagcagatta cttcattggc
      781 aattttgtgg gcattgggta ggttttcctt agctgccgag ggtaagcctc tatacgtaga
      841 aaaatcgctg atcaagggat aaaagtatcg gcgtccgtaa atattgttcg cctttaattt
      901 ttcgtacagc tcatcacgac tcattccgta ttcattttca tcgacaaaaa taggaaaata
      961 ggaataatta tgttttacac caggaaaatc tttcaaaaag gtgattcctt ttatctctct
     1021 cagttgttct ctgtattgtt gggcaatggc ttttcgtttt gcgatggcat cgtccacgta
     1081 tttcaattgc agcagcccga aagcagcacg cacttcatcc atctttccgt tgattcccgg
     1141 agccacaacg gtggtttctc cggcgaatcc gaaatttttc agataatcga tgcgttgctt
     1201 tgtttttgcg tcatgacaca ctaaagcacc tccttcgaaa gtgttatagg ttttagtagc
     1261 atgaaaactc aaggtagcca aatcgccggc attcaatatt gattctccat ttacttgtac
     1321 cccaaaagaa tgagcggcat cataaattac tttaagccca tacgtatcgg ctatttcttc
     1381 tattctttta gtgtcgcaag gatttccata gacatgaacc ggcatgatag cggttgtttt
     1441 gggagttatg gcagcttcaa tcttttccgg atcaagattt ccggttttgg ggtcaatatc
     1501 cacaaagacc ggtttaatgc cattccacca caacgaatgt gtggtagcta caaaactaaa
     1561 aggggtagta attacttctc cggttattcg caatgcctgc aatgccacca tcaaaggcaa
     1621 tgttccattc gtaaacaaac tgatataatc cacatgaaga tattcgcata aggttttttc
     1681 caattcctga tgaaattgtc cattattggt gagccatttg ctttcccata ttttttccag
     1741 ataaggaata aattcttcca gtggaggtaa aaatggagaa gtaaccgtta tttgacgatt
     1801 cattttcaat aaaattaaat ttaaaagtga tagttcttga agttacataa ttagaattat
     1861 taattagaaa ctattttcaa atttatgagg gaacatcaaa ctttttcatt ccttattcat
     1921 ttaacgttga aactatttct taaataggtg tagtttaaaa cccaataaat tgaatacgaa
     1981 tgtagtaatt tattttgaat gtgaaatatt tttatctgaa aaaaggaagg aatttttata
     2041 acattgtcta aacggtattt aaacaaagat taaaaagcat tgtagtgcgt agcagaatcg
     2101 aactgctatt atcagattga gaaactgacc tcc
//
LOCUS       DAOTKS010000290         2132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_41705_length_2132_cov_4.894078, whole genome shotgun sequence.
ACCESSION   DAOTKS010000290 DAOTKS010000000
VERSION     DAOTKS010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41705_length_2132_cov_4.894078"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="P5236_05825"
     CDS             complement(<1..1158)
                     /locus_tag="P5236_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HRU63917.1"
                     /translation="MQFKIYFSFILFFFSFSFLTAQINDSLFIKSLADTINLTVDTTQ
                     VPEIEYSNYSQKYQIAKITVTGAENYEDFVLIGYSGLSVGDTISIPGEEITNAVKRFW
                     KQGLFSDVKIYATKIEDGKVWLNIDLKPQPRISKINYQGLKKGEIEDLMKKLNLSEGS
                     QITPNIIDRIKTITKNYLEEKGFLNSEVNVYTQDDPEKPGYVTVDIEVDKKIKIGVHK
                     IYVTGNKALTHAQVDKAMKKTNEKHIRNLFRSKKFVREEYEKDKIALIEKYNEIGYRD
                     AYIVADSVVPFNEKSVDIYITVNEGEKYYFRDIKWIGNTVYPYELLSAQLKINKGDVY
                     NYKYLMDRLQNDDDAISKFYQNNGYLFFNIEPVEVQIDNDSIDLEIHIYEGK"
     gene            complement(1189..1926)
                     /locus_tag="P5236_05830"
     CDS             complement(1189..1926)
                     /locus_tag="P5236_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HRU63918.1"
                     /translation="MSSFKEKIDYQSLPQHIAIIMDGNGRWAKERGYDRIYGHQHGVT
                     SVRNVTEASAELGIPYLTLYAFSTENWNRPLEEVNALMELFVSTIENETAELQKNNIK
                     LSTIGDLNRLPKKVKQQLLNCQEITNQNTRLNLILAVSYSSRWEITTAARKIAKDVET
                     KKLTLEEINDRLFSNYLETKLIPDPDLMIRTGGEIRISNFLLWQLAYTELYFTPKQWP
                     DFDKESFYEAIYEFQQRERRFGKIVDF"
     gene            complement(1951..>2132)
                     /locus_tag="P5236_05835"
     CDS             complement(1951..>2132)
                     /locus_tag="P5236_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63919.1"
                     /translation="AQWGFRWISADDIEAQEPLNNYYQLNGSNPFNKDFVSTLMVGLT
                     FDFLKKPCDCKVITY"
BASE COUNT          683 a          349 c          292 g          808 t
ORIGIN      
        1 ttttccttcg taaatatgga tttcaagatc gattgaatca ttgtcaattt gtacttctac
       61 aggttctatg ttgaaaaaaa gataaccatt gttttgataa aacttgctaa tggcatcatc
      121 gtcattttgc aagcgatcca ttaaatattt ataattataa acatcacctt tgttaatttt
      181 caattgagca cttaatagct cgtaaggata aacggtattt ccaatccatt ttatatctct
      241 gaaataatat ttttcccctt catttacagt gatataaatg tcaacgctct tttcattgaa
      301 aggtaccaca ctatccgcta caatataagc atcacgataa ccgatttcgt tatatttttc
      361 aattaaagca attttatctt tttcatattc ctcacggaca aattttttag atcgaaataa
      421 atttctgata tgtttttcat tggttttctt catggcttta tccacttgag catgtgttaa
      481 agctttattt ccagtaacat aaattttatg aacgccaatt tttatttttt tgtccacctc
      541 aatatctacc gttacatatc ctggcttttc gggatcatcc tgtgtgtaaa catttacttc
      601 tgaattaagg aaaccttttt cttctaaata atttttggta attgttttga tcctgtcaat
      661 aatattagga gtaatttgac tcccttcgga tagattcaat tttttcatta aatcttcgat
      721 ttctcctttt tttagtcctt gatagttaat tttagaaatt cgtggttgag gtttcagatc
      781 aatatttagc catacttttc catcttcgat tttagttgcg taaattttta catcagagaa
      841 taatccttgt ttccaaaaac gttttactgc attcgtaatt tcttctcctg gaatgctaat
      901 ggtatcgcct accgataaac ctgaatatcc aatcaagaca aaatcttcat agttttcagc
      961 tcctgtaacg gttatttttg caatttgata tttttggctg taattagaat attctatttc
     1021 tggcacttga gtagtatcaa cagttaaatt aattgtatca gcaagtgatt taatgaaaag
     1081 agaatcattg atttgagctg tcaaaaatga aaaagaaaaa aagaaaagta taaaagaaaa
     1141 atatatttta aattgcataa taatttttaa gatagaattt taaaaaaatt aaaaatcaac
     1201 tattttacca aagcgtcgtt ctctttgttg aaattcataa atagcttcat aaaaagattc
     1261 tttgtcaaaa tcaggccatt gtttgggagt aaaatataat tcagtatagg ctaattgcca
     1321 caataaaaag ttactgattc taatttctcc tcctgttcta atcataaggt ctggatcggg
     1381 tattaatttg gtttctagat aatttgaaaa taatcgatca ttgatttctt ctaaggttaa
     1441 tttttttgtt tcaacatctt tggcaatttt tcttgccgca gtggtgattt cccaacgaga
     1501 agaataactg actgccaata ttaaattaag tcgcgtattc tggtttgtaa tttcttgaca
     1561 attaagcaat tgttgtttta ctttttttgg taaccgattc aaatcaccaa tggttgaaag
     1621 tttaatattg tttttttgaa gttctgccgt ttcattttca atggtactca caaacaattc
     1681 cattaaagca tttacttctt caaggggacg attccaattt tcagtagaga aagcatataa
     1741 agtaagatat ggaataccaa gttcagcaga agcttctgtc acatttctaa cagaagttac
     1801 accatgttga tgcccataaa ttctatcata acctctctct tttgcccaac gcccattacc
     1861 atccattatg attgctatat gttgaggcaa actttgataa tcgatttttt ctttaaaaga
     1921 tgacataaat gaacgacata taaatttgaa ttaataagtt atcactttac aatcacaagg
     1981 ttttttcaga aaatcaaaag taagtcctac cattaaagta gaaacaaaat ctttattaaa
     2041 tggattgctt ccgttcagtt gataataatt atttaatggt tcttgagctt caatatcatc
     2101 agcactaatc caacgaaaac cccactgtgc at
//
LOCUS       DAOTKS010000291         2128 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_41810_length_2128_cov_4.459720, whole genome shotgun sequence.
ACCESSION   DAOTKS010000291 DAOTKS010000000
VERSION     DAOTKS010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41810_length_2128_cov_4.459720"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..1081)
                     /locus_tag="P5236_05840"
     CDS             complement(107..1081)
                     /locus_tag="P5236_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HRU63920.1"
                     /translation="MLYPLKFHPILKEKIWGGSRLKKLFNKPTESDKIGESWELSGYP
                     GDESVVANGFLAGNQLNELIEVYMDELVGGKVFDRYGLTFPLLFKFIDANDDLSIQVH
                     PNDETALKKHDSLGKTEMWYVVDADENGELIVGFKNDCSAAEFLTALNEKNLEDLLQR
                     VSVKKGDVIFIHPGLVHAIKKGVVVAEIQENSDLTYRIYDYQRKDENGKERDLHLEAA
                     LEVIDFSACKNPLVDYVEKMNEVTTLVSCQYFTTNLLAFNQHFLRNYVQIDSFVVYMC
                     LEGNFIIEYENSRTPVEKGETVLIPASMDEVNLIPAEGTVKLLEVYID"
     gene            complement(1097..>2128)
                     /gene="galE"
                     /locus_tag="P5236_05845"
     CDS             complement(1097..>2128)
                     /gene="galE"
                     /locus_tag="P5236_05845"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRU63921.1"
                     /translation="TGYIGSHTVVELQQEGFEVIIVDNLCNSNIEVLNGIEKITGIRP
                     MFENIDCTDYINMDRMFEKYQNIKAIVHFAALKAVGESVEKPLMYYRNNLVSLINLLQ
                     LMPVHKIKNIVFSSSCTVYGQPDVLPVREDTPFKPALSPYGNTKQICEEIIRDTIHAN
                     PNLYRSIVLRYFNPIGAHPSAEIGELPIGVPNNLLPFITQTAIGIRKQLLVYGNDYNT
                     PDGTCIRDYIDVVDLAKAHVIALKRMLKQQQKTNLEVFNLGTGRGLSVLEIIKTFEKV
                     NGVKVPYKIVGRRPGDIEKVWADPSLANKELGWKAERTLEETLRSAWEWEKKLASRAK
                     EKDHTKTES"
BASE COUNT          687 a          408 c          307 g          726 t
ORIGIN      
        1 ttccaaaagt tccgggaaaa ttatttgtta cagaaaaata aaatcactta actattgatt
       61 aacagaaaac agcagaaaaa cttgaaaatt tttctgctgt tttttatcag tctatataca
      121 cttccagtaa ttttacggtt ccttcagcag gaattaaatt aacttcatcc attgaagcag
      181 gaatcaagac tgtttctcct ttttcaactg gtgttcggct attttcatat tcaataataa
      241 aatttccttc caagcacata tagactacaa atgagtctat ttgaacataa tttcgtaaaa
      301 aatgttgatt aaaggcaagt aaattggtag taaaatattg acaggaaacc aatgtggtaa
      361 cttcattcat tttttctaca taatctacga gtggattctt acatgcactg aaatcaatca
      421 cttcaagagc ggcttcaaga tgtaaatccc tttctttccc attttcgtct ttccgttgat
      481 agtcataaat acgataagta agatcactat tttcttgaat ttcagcaaca accactcctt
      541 tttttattgc gtgtaccaaa cctggatgaa taaaaataac atctcctttt tttaccgaaa
      601 cacgttgtaa taaatcttca aggttttttt cgtttaaagc tgttaaaaat tccgccgctg
      661 aacaatcatt cttaaaacca actataagct ctccgttttc atccgcatct accacatacc
      721 acatttcagt ttttcctaat gagtcatgtt tttttaaagc tgtttcatca ttaggatgta
      781 cttgaataga caaatcgtca ttggcatcaa taaatttaaa aaggagaggg aaggttaatc
      841 catagcgatc aaatactttg ccaccaacca attcgtccat ataaacttct attaattcat
      901 tgagctgatt gccagctaaa aaaccattcg ctacaacgga ttcatcacct ggataacctg
      961 ataattccca gctttcacca attttgtcgg actcggtagg cttattaaat aattttttta
     1021 gacgactgcc tccccaaatt ttttctttta aaataggatg aaattttaat ggatatagca
     1081 ttattttaaa aagtgattaa gattcagttt tggtatgatc tttttctttt gcacggcttg
     1141 ccaatttctt ttcccattcc cacgcactac gcaaagtctc ttccagtgtc ctttcagctt
     1201 tccaacctaa ttccttattt gccagagagg gatcagccca aactttttca atatctccag
     1261 gtcggcgtcc tacaatttta taaggaactt tcacaccatt tactttttca aaagttttga
     1321 taatttccag tacggataaa cctcttccag ttcccaaatt gaaaacttca agattcgttt
     1381 tttgttgttg ttttagcatg cgttttaaag caataacatg agctttggct aaatcaacta
     1441 catcaatgta atcacgaata caagtgccat caggcgtgtt ataatcattt ccataaacca
     1501 gaagctgttt acgaatacca atagctgttt gggtaataaa tggtaataaa ttattaggaa
     1561 caccgattgg taattcacca atttcagccg aaggatgagc accgattgga ttaaaatatc
     1621 tcaatacaat acttcggtaa aggttaggat tggcatgaat agtgtctctt atgatttctt
     1681 cacaaatttg ttttgtatta ccataaggtg ataacgctgg tttaaaaggg gtatcttctc
     1741 taacaggcaa aacatcaggc tgtccataaa ccgtacatga agatgaaaaa acgatattct
     1801 ttatcttgtg gacaggcatc aattggagaa gattgattaa agaaaccaaa ttgttacgat
     1861 aatacattaa aggtttttcc accgattctc ctaccgcttt taaagctgca aaatgaacaa
     1921 ttgctttaat attttgatat ttttcaaaca ttcgatccat atttatataa tccgtgcagt
     1981 caatattttc aaacatgggt cgaatgcccg taattttttc aataccattt aatacttcta
     2041 tgttggagtt acaaagatta tcaacaataa tcacttcaaa accttcctgc tgtaattcta
     2101 ctacagtatg agaaccaata taacctgt
//
LOCUS       DAOTKS010000292         2128 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_41813_length_2128_cov_3.515678, whole genome shotgun sequence.
ACCESSION   DAOTKS010000292 DAOTKS010000000
VERSION     DAOTKS010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41813_length_2128_cov_3.515678"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..1513)
                     /gene="cls"
                     /locus_tag="P5236_05850"
     CDS             complement(80..1513)
                     /gene="cls"
                     /locus_tag="P5236_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HRU63922.1"
                     /translation="MSKFITIFFIALYVYTVLSTISVLLLENRNPAKSFSWMLIILFL
                     PVIGLLLYITFGQDLRKQKIISRKSLHNLPERVIAPFDITEVEQLLNNLEKCQKNLIK
                     LLYKNSEAIAYPANKIQVYPDGESAFDAFFDAIREAKNHIHIEFYILSNDKISQQLKE
                     LLIQKSHEGVRVRVIYDYIGSLNLSRKYIKSLTKAGVYVRPFLPLRLRIFRSRINYRD
                     HRKIMIIDGEIGFTGGMNIADRYIKGNRLGKWRDNFVRFEGAVVHGLQRLFLADWYFV
                     EGKLITDTKYYPQPENFNTNNLIQIVSSGPDTDWESIMQGIALAIMTASKYVYIQSPY
                     YMPPELIECCIQIASLSGVDVRLMIPTRSDSIIAIKSTSSYLGKILEAGVKVYYYKTG
                     FLHSKALVIDDMISIVGSCNIDYRSFTQNFEANAFIYEKETAIQLKELFIKDIQQCEE
                     MTLEKWANRSKFQKMEESFSRLFSPIL"
     gene            complement(1521..>2128)
                     /locus_tag="P5236_05855"
     CDS             complement(1521..>2128)
                     /locus_tag="P5236_05855"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HRU63923.1"
                     /translation="KKRPFVTLKWAQTIDGFMDKNRTNVQQAPLLISNNFTRVLAHKM
                     RSENQAILVGTNTVLLDNPSLTVRYWSGKTPVRIVLDRLGKIPENYHLLNNSTPTIIF
                     TEKPYQEKLNVKYIPIDFDKNILSTVLQKIYDLNIHSILVEGGAQLLTNFIISNLWDE
                     AYVEVAPFEISEGVTAPIIPLMPFARKNFYGHEWYHYKNKK"
BASE COUNT          716 a          339 c          337 g          736 t
ORIGIN      
        1 agctaatttc ttattcataa ggaaaattat tcaaaggata agtgaaaaat tgttttaata
       61 atgtttttta aataaatatt cataaaattg gactgaaaag acgcgaaaat gattcttcca
      121 ttttctgaaa tttgcttcgg tttgcccatt tttccagtgt catttcttca cattgttgaa
      181 tatcttttat aaaaagttct tttaattgga tagcagtttc tttttcataa ataaaagcat
      241 tcgcttcaaa attctgagta aagcttcgat aatctatatt acatgaacct acaatagaaa
      301 tcatatcatc tatcactaat gccttgctat gcagaaaacc agttttataa tagtaaactt
      361 ttacgcccgc ttccagaatt ttgccaagat aactggaagt gcttttaata gcaataatag
      421 aatcggaacg cgtaggaatc ataagtcgta catcaactcc acttaacgaa gctatctgga
      481 tgcagcattc aattaattct ggtggcatat aataaggcga ttgaatataa acgtatttgc
      541 tggcagtcat aatagctaaa gcaattcctt gcatgatgga ttcccagtcg gtatcgggtc
      601 cactggaaac aatttgaatc aaattattgg tgttgaaatt ttcaggttga ggataatatt
      661 tggtatcggt aattaatttt ccttcaacaa aataccagtc ggcaagaaat aaacgttgca
      721 agccatgtac tactgctcct tcaaaccgaa caaaattatc gcgccattta cctagtcgat
      781 tgcctttaat gtatcgatca gcaatgttca ttccgcctgt gaaaccaatt tccccatcaa
      841 taatcataat ttttctgtga tctcggtagt tgattctgct tctaaaaatt cttaaacgta
      901 atggtaaaaa aggacgaaca taaactcctg ctttagtcaa ggattttata tattttcttg
      961 ataagttcaa gcttccaata taatcataaa ttactcggac acgaacacct tcatgagatt
     1021 tttgaatcaa taattctttt aattgttgag aaattttatc attacttaaa atgtaaaatt
     1081 caatatgaat gtggtttttg gcttcgcgaa tggcatcaaa aaaagcatca aaagcacttt
     1141 cgccatccgg gtaaacttga attttattag caggataagc aatggcttca ctgtttttat
     1201 ataaaagttt gatgagattt ttctgacatt tttcaaggtt attcaataat tgttctactt
     1261 ctgtaatatc aaaaggagca atgactcgtt caggtaagtt atgaagactt tttctggaaa
     1321 taattttttg tttgcgtaaa tcttgtccaa aagttatata aagcagcaag ccaattacag
     1381 gaaggaacaa gatgatcagc atccacgaaa atgattttgc tggatttcta ttttcaagca
     1441 acaatacaga aatagtactg agaacagtat agacgtaaag agcaataaag aatatagtaa
     1501 tgaattttga catttcttat ttatttttta ttcttataat gataccattc atgtccataa
     1561 aaattttttc gtgcaaaagg cattagcggt ataataggtg ctgtaacacc ttcactgatt
     1621 tcaaaaggtg ctacttccac ataagcttca tcccataagt tggaaataat aaaatttgtt
     1681 aataattgag cgccgccttc tactaaaatg gaatgaatat tcagatcata aatcttttgt
     1741 aaaacagttg ataagatatt tttatcaaaa tctattggaa tatattttac atttaatttt
     1801 tcctgataag gtttttcagt aaaaattatt gttggtgtag agttatttag taaatgataa
     1861 ttttccggta ttttccccaa gcgatccagc actattctga caggtgtttt tcctgaccaa
     1921 tatcgaacgg ttaaggaagg attatctaaa agaacagtat tagtaccaac aagaattgcc
     1981 tgattttctg aacgcatttt atgagcaaga accctggtaa aattattgga aatcaacaaa
     2041 ggagcttgtt gaacatttgt tctgtttttg tccataaatc catcaatagt ttgagcccat
     2101 tttagtgtaa caaatggacg tttctttt
//
LOCUS       DAOTKS010000293         2127 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_41830_length_2127_cov_4.336390, whole genome shotgun sequence.
ACCESSION   DAOTKS010000293 DAOTKS010000000
VERSION     DAOTKS010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41830_length_2127_cov_4.336390"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2010
                     /locus_tag="P5236_05860"
     CDS             <1..2010
                     /locus_tag="P5236_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRU63924.1"
                     /translation="VYGIASNHFAFPLLVMNKNTFQSATFFTPADAMKMEPGIAMLRD
                     GIWATSVNIRGLSEPRLLFLIDDNRILTTIGMAAALSTVDLNSIEKIEIIKGAGSALF
                     GSGAMGGVVNFITERPAYTSFFQVKGKVGSGLHTVNNLWNNDAKIDLTNENWYLSLKG
                     SYRAAQNIQTPTGKLPNSQFNDVSWGIQGGMSYVSNQELLFNYQHFEAWHVGLLERKT
                     FLDTAMVRYKNVKRNLFSVEYIFSDVSDYLKEVRLKAYTQNFDHDIENIVNPSTVILP
                     SSLNSTWGLKAVSNWRFTEYNKLILGAESWLRNTKTICKKITRGDSVTAVVGEQLTPD
                     AEVFDAGIFGQYRWEFSPHYWVLNAGLRLDYFQISNDTAFQPVYRYTYSNSLPSEQKY
                     VANLPRMILYEAGKHRNFSYAAQVDLTYYPTSIQKFTFSLSNSYRMASIEELFKYIDQ
                     QSGIIRVGNPSLKPEKGIFTNLSYALDNHRFFLKTDVFANYLFDLITEKKGTFTYINS
                     NGIIENREAYINTNINKAILYGFEIDVNWKINSFLSTQGNLSCTFGKDIREDEWLPQI
                     PPLKGILTLNYTQKDLFEISLSAIGAAKQSKVAPEEQPTNGHVIFNANIHSVKIIVGN
                     DNYFQLLVGANNILNTSYHDHLAATRGLICLEPGRDIYFKLNYGW"
BASE COUNT          702 a          314 c          371 g          740 t
ORIGIN      
        1 gtttatggca ttgcttctaa ccattttgct tttccattgt tagtaatgaa taaaaatact
       61 tttcaatcgg ctactttttt tacgccagca gatgctatga aaatggagcc aggaattgca
      121 atgttgcgtg atggaatatg ggcaacctca gtaaacatta gaggattatc agaacctcgt
      181 ttgttgtttc taattgatga taataggatt cttacaacta ttggtatggc agcagcttta
      241 tcaactgttg atttaaacag cattgaaaaa attgaaatta tcaaaggcgc tggttcagcg
      301 ttatttggtt caggagctat gggaggagtt gtaaacttta ttaccgaacg tcctgcttat
      361 acttcctttt ttcaagtaaa aggtaaagtt ggatcaggtt tacataccgt caataattta
      421 tggaacaatg atgcaaaaat agaccttact aatgaaaatt ggtatttatc attgaaagga
      481 agttatcgtg ctgcacaaaa tattcaaacg ccaacaggaa aattacctaa tagtcagttt
      541 aatgatgttt catggggtat tcaaggtgga atgagttatg tatcaaatca ggagttatta
      601 tttaattatc agcattttga agcgtggcat gttgggctac tggaaagaaa aacatttctt
      661 gatacagcta tggtacgtta caaaaacgta aaaagaaatt tattcagtgt tgaatatata
      721 ttttctgatg tgagcgacta tcttaaagaa gttcgtctga aagcttatac ccaaaatttt
      781 gatcacgaca ttgaaaatat tgtaaatcca tcaactgtta ttctaccttc gagcctaaat
      841 tcaacgtggg gtttaaaagc agtttccaat tggcgattta ccgaatataa taaattaata
      901 cttggagctg aaagctggct tcgtaatacc aagactattt gcaaaaaaat aacaagagga
      961 gactctgtta ccgctgttgt tggcgagcag ctaactcctg atgctgaagt gtttgatgct
     1021 ggaatttttg gacaatatcg gtgggaattt tctccccatt attgggtttt gaatgcggga
     1081 ttaaggctgg attatttcca aatttctaat gacactgcct ttcaacccgt ttatagatac
     1141 acctattcga acagtttgcc atctgaacaa aaatatgttg caaatcttcc tcgaatgatt
     1201 ttatatgaag cggggaaaca tcgtaatttt tcttatgctg ctcaagttga tttaacttat
     1261 tatcctactt ccatacaaaa atttactttt tctttgtcaa attcttatag aatggcttct
     1321 atagaagaat tattcaaata tattgatcaa caatcaggaa taataagagt gggaaatccc
     1381 tccttgaaac ctgaaaaagg aatttttacc aatttaagtt atgcgttaga taatcatcgt
     1441 tttttcctga aaacagatgt ttttgccaac tatttatttg atttgattac tgaaaagaag
     1501 ggaacattta cttatataaa ttcaaatggt atcattgaaa atcgtgaagc ttatatcaat
     1561 actaatataa ataaagctat attatatgga tttgaaatag atgtaaattg gaaaattaat
     1621 tctttccttt ctacacaggg aaatttatcc tgtacgtttg ggaaagatat tagggaagat
     1681 gaatggttac cacaaattcc tcctttgaaa ggtattctta ctttaaatta tacacaaaaa
     1741 gacttgtttg aaatttcatt atcggcgata ggagcagcca agcaatcaaa agttgctcca
     1801 gaagaacaac caactaatgg acatgttatt tttaatgcca acattcattc tgtaaaaatc
     1861 attgtgggga atgataatta tttccaatta ttagtgggtg caaataatat tttgaatact
     1921 tcctatcatg atcatcttgc tgcgacaaga ggtcttatct gcttagaacc aggaagagat
     1981 atttatttca aactcaatta tggttggtaa atattttttc tttaaattat taactttcat
     2041 tcactttaaa attaatttat tttgctcatt atcggtatta tattagtatt tattgtattt
     2101 atagctgctt tttttaaaag atggaat
//
LOCUS       DAOTKS010000294         2114 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_42179_length_2114_cov_2.433706, whole genome shotgun sequence.
ACCESSION   DAOTKS010000294 DAOTKS010000000
VERSION     DAOTKS010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42179_length_2114_cov_2.433706"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="P5236_05865"
     CDS             <1..945
                     /locus_tag="P5236_05865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRU63925.1"
                     /translation="GFPVNATSLQQRIYPGNAFPSQQSILDGNKKHRVVKVQCTWAIP
                     QDTTLYAVYTDGTVYNSNPNCSNSLNGTVTEGWLMLDDFEAGNIDDTYAVYKAENTTS
                     STTAIVKADPVVTSNQVVLFSNQNYGHYLQLNVTLPSGKKLSDYEKISFDVYCPTGMG
                     TYQTLRMGINQTLADLTNNGSNTLTSWTTLERDIPTAFSADADLSTFTMELGFRTAAK
                     DYYIDNIKLKEVIGLPTEINETTFNQIKSPFFYSRNIITTVNENIADFDIYDIEGRLL
                     KVAYQVSTIDISDLNVGIYLIKMNMSGKLFIEKIVKNR"
     gene            1363..>2114
                     /locus_tag="P5236_05870"
     CDS             1363..>2114
                     /locus_tag="P5236_05870"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HRU63926.1"
                     /translation="MFEEKHHILIFILLILSNSVFPQHDKIPSIKKTNGVFQFIVKEK
                     PYLMLGGELSNSATSDVEMMKGIWGKLKDMNLNTVLAPVYWELIEPEEDKFDFTLVDT
                     MINQAREKDLHLVLLWFGSWKNSMSCYVPEWIKEDYKRFPHTLDADGKPAEIVSPFCK
                     EALTADIKAFSELMKHIKSVDEIEQTVIMMQVENEIGQLPDARDYSTLANRAFQADVP
                     KELMDYLSKNRKNLLPSIENLWASNGYKQKGN"
BASE COUNT          751 a          302 c          390 g          671 t
ORIGIN      
        1 ggattccctg tcaatgcaac ttccttacaa caaagaattt atcctggaaa tgcttttcct
       61 tctcaacaat ctattctgga tgggaataaa aaacatcggg ttgtcaaagt gcaatgtaca
      121 tgggcaattc ctcaagacac tactctttat gctgtttata ctgacggaac ggtttacaac
      181 tccaatccaa attgtagtaa tagtttaaat ggtacggtaa cggaaggctg gttgatgtta
      241 gatgattttg aagctggcaa tattgatgat acttatgccg tgtataaagc agaaaataca
      301 acatcatcaa caacagcaat agtgaaagca gatcctgttg ttaccagcaa tcaggtggtc
      361 cttttttcca atcaaaatta tggacattat ctacagttaa acgtaactct tcccagtgga
      421 aaaaaattaa gcgattatga aaaaatatca tttgatgtat attgtccaac aggaatggga
      481 acttatcaaa ctttgagaat gggtattaat caaacgttgg cagatttgac aaataatggt
      541 agtaatacac ttacttcttg gacaactttg gaaagagata tacctacggc tttctctgcg
      601 gatgctgatt tatcaacatt tacgatggag cttggatttc gtactgctgc taaagattat
      661 tatatagata acataaaatt aaaagaagta ataggactac ctactgaaat aaatgagacg
      721 acatttaatc aaattaaatc tccatttttt tattcacgta atattataac tactgtaaat
      781 gagaatattg cagattttga tatatatgat attgaagggc gtttattaaa agttgcgtat
      841 caagtttcaa ccatagatat ttctgatctt aatgttggta tttatctaat taagatgaat
      901 atgtcaggga aattgtttat tgagaaaatt gtaaagaata ggtaatttaa ataaaaattt
      961 aaattagatt ttgcaaaaat tctaaaagta agttagttga cacaatttat gcattaaaaa
     1021 aatataatta atcatcgttg cagcttataa gacgagtatt taattgatca ttgataaaga
     1081 ataatttaat cttaaaaacg tcttggcagg aaaagatgtg agtgccgatt tcacgggaga
     1141 gctacagtat tagaatattg ataaagtttt ttgatgtaat ttgaaaaaca gattggagcc
     1201 aaggagtaat ttgaaaaatc aggcatcaaa atccatgata ttaaaatgat tgaattaact
     1261 gattggaaaa tcaatttgat tttccaatca gtttttaaaa tgcaaaattc tattcgtctg
     1321 atttgtaata acaaatgcaa ttttattgat taatttttca atatgtttga agagaagcat
     1381 catattttaa tttttatttt attgatcctg tccaatagtg tttttcctca acacgataaa
     1441 ataccttcaa taaaaaagac gaatggcgtt tttcagttta ttgtaaaaga gaaaccttac
     1501 cttatgttag gtggtgaatt gagcaattct gcaacgagcg atgtggaaat gatgaaaggt
     1561 atttggggca aattaaagga tatgaatctg aatacggttt tagctcccgt ttattgggaa
     1621 ttgatcgaac cagaggagga caagttcgac tttacattgg tcgacaccat gatcaatcaa
     1681 gctcgggaaa aagatctgca ccttgttctt ctttggtttg gctcgtggaa aaacagcatg
     1741 tcctgttatg taccggaatg gatcaaggaa gattataaac ggtttcccca cacgttggat
     1801 gccgacggaa aaccagcaga aattgttagt cccttctgta aagaggcttt aactgcggat
     1861 atcaaagcat ttagcgaatt gatgaaacat ataaaatctg tcgatgaaat agaacaaaca
     1921 gtcattatga tgcaagtgga aaatgaaatt ggacaacttc cggatgcacg cgattattcc
     1981 actttggcta atagggcatt tcaagcagat gttcctaaag aattaatgga ttatttatcc
     2041 aaaaacagaa aaaatttact cccttcgatt gaaaatttgt gggcttcaaa cggatataaa
     2101 caaaaaggga attg
//
LOCUS       DAOTKS010000295         2113 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_42193_length_2113_cov_3.854713, whole genome shotgun sequence.
ACCESSION   DAOTKS010000295 DAOTKS010000000
VERSION     DAOTKS010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42193_length_2113_cov_3.854713"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1654)
                     /gene="rpoB"
                     /locus_tag="P5236_05875"
     CDS             complement(<1..1654)
                     /gene="rpoB"
                     /locus_tag="P5236_05875"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212060.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRU63927.1"
                     /translation="MSSLTNTERINFSTIKNQLEYPDFLDIQLKSFQDFFQMDTPPEK
                     RKLEGLYKVFTENFPVSDTRNNFVLEFLDYFIDPPRYSIEECIERGLTYNVNLKAKLK
                     LYCTDPEHEDFDTVIQDVFLGPIPYMTPRGTFVINGAERVIVSQLHRSPGVFFGESLH
                     ANGSKLFSARIIPFRGSWIEFATDINNIMYAYIDRKKKLPVTTLLRAIGFESDKDILE
                     IFDLAEEVKVSRSNLKKYIGRKLAARVLKSWLEDFVDEDTGEVVTIERNEIIIDRETV
                     LENEHIDLILEAGAQTILLHKEEADQIDYSIIYNTLQKDPTNSEKEAVLYIYRQLRNA
                     EPPDDATAREVINNLFFSEKRYDLGDVGRFRINQKLNLNISNETKVLTKEDIIEIIKY
                     LVLLINSKSTVDDIDHLSNRRVRTIGEQLYNQFNVALARMVRTIRERMNVRDNEVFTP
                     VDLINVKTISSVINSFFGTNALSQFMDQQNPLAEITHKRRMSALGPGGLSRERAGFEV
                     RDVHYTHYGRLCPIETPEGPNIGLISSLCVYAKINELGFIETP"
     gene            complement(1771..>2113)
                     /gene="rplL"
                     /locus_tag="P5236_05880"
     CDS             complement(1771..>2113)
                     /gene="rplL"
                     /locus_tag="P5236_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRU63928.1"
                     /translation="NLTVKEVNELAQILKEEYGIEPAAATVAVSNAPAAAAAEEKPEK
                     TSFDVVLKSPGPNKLAVVKLVKDLTGLGLKDAKDLVDSAPSVVKEGLSKEEAETLQKQ
                     LSDGGAEVELK"
BASE COUNT          656 a          389 c          345 g          723 t
ORIGIN      
        1 agggcgtttc aataaaacct aattcattga tttttgcata aacacacaga gaagaaatca
       61 atccaatatt aggtccttca ggtgtttcaa taggacataa acgcccgtaa tgagtataat
      121 gtacatctcg aacttcaaag cctgcacgtt cacgtgataa gcctccggga cccaaagctg
      181 acatcctacg tttatgggta atttctgcta atggattttg ttgatccata aattgtgata
      241 atgcattggt accaaaaaaa gagttgataa ccgaagagat tgttttaaca ttaatcaaat
      301 ctactggagt aaatacttcg ttgtctcgta cattcattcg ctcacgtatg gtacgtacca
      361 tacgggctaa ggctacatta aattgattgt ataactgctc tccaatagtg cgaactcttc
      421 gattactcaa atggtcaata tcatcaaccg tagattttga attaatcagt aaaacgagat
      481 acttaataat ttcaataata tcttctttgg tcaggacctt tgtttcatta ctaatattca
      541 agtttaattt ttggttgatt ctaaatcgtc ctacatctcc caagtcgtac cttttttcag
      601 aaaagaataa attattgatc acttcacgag ctgtagcatc atcaggtggt tctgcatttc
      661 gtaattgcct gtaaatatag agcacagctt ccttttccga attggtcgga tctttttgca
      721 aagtattata aataatacta tagtcaatct gatctgcttc ttctttatgt aaaagaattg
      781 tttgagcacc cgcttcgagt attaaatcaa tgtgctcatt ctccagcact gtttcacgat
      841 caataatgat ttcgtttcgc tcaatggtaa caacttcacc cgtgtcctca tctacaaaat
      901 cctcgagcca gctttttaaa acacgggcag ccaatttacg ccctatatat ttttttagat
      961 tcgatctgga tacttttact tcttcagcaa gatcaaaaat ttccagaata tctttatcgc
     1021 tttcgaaacc aatagcacga agcaaggtag tgacaggaag ttttttcttt ctgtcaatat
     1081 aagcatacat gatattatta atatcagttg caaattctat ccatgagcca cggaaaggaa
     1141 taatacgtgc tgaaaataat ttacttccat tagcatggag actttctcca aaaaatacac
     1201 cgggtgaccg atggagttga gaaactatta ctctttcagc accattaatt acgaaagtgc
     1261 ctcttggagt catataggga atagggccta aaaatacatc ttgaataaca gtatcaaaat
     1321 cttcatgttc cggatccgta caataaagtt ttaattttgc ttttaaattg acattataag
     1381 ttaaacctcg ttcaatacat tcttctatgg aataacgcgg tggatctata aaataatcta
     1441 aaaattcaag aacaaaatta ttacgtgtat cagaaacagg aaagttttca gtaaatactt
     1501 tatataaccc ttctagtttt cttttttcag gaggagtatc catctggaag aaatcctgga
     1561 aagattttaa ttgtatgtct aagaaatcag gatactcaag ctgatttttt attgtggaaa
     1621 aattaattct ttcagtgtta gttaatgaag acatttttca aagaggttat ttttgttcca
     1681 aagatctttt atcatgttag ataggaaaag gtttagaacc ccctttttat agggagtcta
     1741 aaccaataag ttaataatca gcagttaata ttatttaagt tcaacttcag ctccgccgtc
     1801 actaagttgt ttttgcaagg tttcagcttc ttcttttgaa agaccttctt taactactga
     1861 aggagctgag tcaaccaagt ctttagcatc tttcaatcca agtcctgtaa gatcttttac
     1921 taatttaact actgcaagtt tattgggacc aggagatttt aatactacat caaaagatgt
     1981 tttttcgggt ttttcttcag cggctgctgc agcaggagca ttgcttacag ccactgttgc
     2041 tgctgctggt tcgattccgt attcttcttt taaaatttga gctaactcgt ttacttcttt
     2101 aactgttaag tta
//
LOCUS       DAOTKS010000296         2108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_42347_length_2108_cov_2.854847, whole genome shotgun sequence.
ACCESSION   DAOTKS010000296 DAOTKS010000000
VERSION     DAOTKS010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42347_length_2108_cov_2.854847"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            398..>2108
                     /locus_tag="P5236_05885"
     CDS             398..>2108
                     /locus_tag="P5236_05885"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRU63929.1"
                     /translation="MKKNLFSLLVFLFLLSFYHLFSQNNEYPIKIINDIEYYLYTVEP
                     AEGTYAISKKFGITQEEILKANPSIENGLKAGQIILIPVKKTNLEKEQSSYNHRTDSS
                     TTSTIQFIQYKVEKKQTLFGISRLYNVSQEEIIKFNPQVVKGLREGEVLFIPKKDNKP
                     EKIIKTDTLAKNNYPEPEKIQKGDSIYFIHLVEGGETLYSISRKYNVEVIDIIKYNPS
                     SAEILKAGSKLKIPVLNVQPDLNQLSTTEKIIQKAISEDETVSKQEKILKIAFLLPFM
                     LNDTKSQLSRENFIDFYAGSLLAIKKGKEKNISMEIFTYDTEKSEEKIKKILAQPELK
                     NIDLIVGPAYSTQVSYVTEFALKNKINNLIPFSSKVYDVVYNPYVFQFNPNNRNVKLK
                     FLYDKLYEKFKNPNIIFAEIPDINILNDNYQLMEEFKKMLANNHKKFTVFSLSSKSSS
                     IENILKKDVDNLIFFNTDNISGVSPYLSDLLNINDKYNIILYEEYSWGNNSNIQKLKG
                     FYVAPFKPDIDAEKLKEYEDEFNRFFNWEITSKLPRYDLLGYDLMNCFIPTLHLYGNS
                     LNTQ"
BASE COUNT          802 a          315 c          296 g          695 t
ORIGIN      
        1 tcgttgtcgg taaagacagt tgtttatttt gcttattatg gagtactatc gtttaatatt
       61 tcaccacaaa gttaaagaac ttcaaaaaat attgaaaaaa ccatttgaag aaatatcttc
      121 aataactttg tgcaccgata ccttgttttc cttattggga acatatttac tgaaacggat
      181 gttgcatcac tgcaactggt tatccatata aggtaaatat ttatgatttt atcatcgtat
      241 ggtaagtcgc tcaagttatt gtgacattga tttttatttt cacgcgtttg gaaatacttc
      301 gattttcatg taattttgca aaagtatatt attaaccaaa catttcacgt tttattattt
      361 ttcaagctta agaataaaaa accatcaaaa taccgtcatg aagaaaaatt tattcagttt
      421 attggtattt ctgtttttat tatcttttta tcacttgttt agtcaaaata atgaatatcc
      481 aattaaaatc attaatgata ttgaatatta tctttacact gtggaacctg ctgagggaac
      541 ttatgctatc agcaaaaaat ttggcatcac gcaagaggaa attctcaaag ccaatccttc
      601 tatcgaaaac ggtctaaaag ctgggcaaat tattttaatc ccggtaaaaa aaacaaattt
      661 agaaaaagaa caatcttctt ataaccatcg aacagatagc tctacaactt caaccattca
      721 atttattcag tataaagtag aaaaaaaaca aacacttttc ggtatcagcc gcctctataa
      781 cgtaagtcag gaagaaatta taaaatttaa tccgcaggtt gtaaaaggtt tacgtgaagg
      841 cgaagtatta tttataccta aaaaagacaa caaaccagaa aaaataatca aaactgatac
      901 gcttgcaaaa aataattatc cagaacctga aaaaatacaa aaaggagata gtatttattt
      961 tattcatttg gtcgaaggag gtgaaacttt atactccatt agccgaaaat ataatgttga
     1021 agtaattgat attataaaat acaatccttc ttcggctgaa attttaaaag cgggaagtaa
     1081 actaaaaatt ccggttttaa atgttcaacc ggacttaaat caactttcga ctactgaaaa
     1141 aattatacaa aaggcaataa gtgaagacga aacggtcagc aaacaagaaa aaatccttaa
     1201 aattgctttc ctcctgcctt ttatgcttaa tgataccaaa tcgcagctaa gccgtgaaaa
     1261 ttttattgac ttttatgcag ggagcttgtt ggctattaaa aaaggcaagg aaaagaatat
     1321 ttcaatggaa atatttactt atgatactga aaaatcagaa gaaaaaataa aaaaaattct
     1381 tgctcagcca gaacttaaaa acatagattt aatagtggga cctgcttata gcactcaggt
     1441 ttcttacgta acagagtttg ctctaaaaaa taaaattaat aacttaatac ctttttcttc
     1501 aaaagtttac gacgttgttt acaatcctta tgtatttcaa ttcaatccta acaacagaaa
     1561 tgtaaaactg aagtttctat atgataaact ctatgagaaa tttaaaaatc caaatattat
     1621 ttttgctgaa attcctgata ttaacatttt aaatgacaac taccagttaa tggaagaatt
     1681 taagaaaatg ttagccaaca atcacaaaaa atttacagtt ttttctcttt cctccaaatc
     1741 ttcctcaata gagaatatat taaaaaagga tgttgataat ctgatatttt tcaatacaga
     1801 caatatatca ggagtaagtc cctatttatc agatttactg aatattaacg acaaatataa
     1861 tattattttg tatgaagaat acagttgggg taataattct aatattcaaa aacttaaggg
     1921 tttttatgtg gctcctttta aacctgacat agatgctgaa aaattgaagg aatatgaaga
     1981 tgaattcaat cgttttttca actgggaaat aacttcgaaa ttgcctcgct acgatcttct
     2041 aggatatgat ttaatgaatt gttttattcc aactttacat ctttatggta atagtttaaa
     2101 tactcaat
//
LOCUS       DAOTKS010000297         2095 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_42699_length_2095_cov_5.520098, whole genome shotgun sequence.
ACCESSION   DAOTKS010000297 DAOTKS010000000
VERSION     DAOTKS010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42699_length_2095_cov_5.520098"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /gene="fusA"
                     /locus_tag="P5236_05890"
     CDS             <1..952
                     /gene="fusA"
                     /locus_tag="P5236_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853959.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRU63930.1"
                     /translation="DTLCDEKHPIILESIDFPEPVIGISVEPKTQKDLDRLSVGLSKL
                     AEEDPTFQVKTDEQSGQTIISGMGELHLEIILDHLRREFKVECNQGRPQVAYKEAITK
                     PVEIREVYKKQTGGRGKFADIIVRVEPADADFEGSLQFIDLVKGGNIPKEYIPAIQKG
                     FLSAMKNGVLAGFPVDKLKVTVLDGSYHPVDSDQLSFEICAQIAFKNACIKANPVLLE
                     PIMKLEVVTPEENMGDVIADLNKRRGQIENTESSHTGARIIKAKVPLAEMFGYVTVLR
                     TITSGRATSSMEFDHYEEVSNSIAKQVVGEAKGKVELLNY"
     gene            1034..1339
                     /gene="rpsJ"
                     /locus_tag="P5236_05895"
     CDS             1034..1339
                     /gene="rpsJ"
                     /locus_tag="P5236_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRU63931.1"
                     /translation="MSQKIRIKLKSYDYNLVDKSAEKIVKTVKATGAIVSGPIPLPTH
                     KRIFTVNRSTFVNKKSREQFELSSYKRLIDIYSSTSKTVDALMKLELPSGVEVEIKV"
     gene            1429..2046
                     /gene="rplC"
                     /locus_tag="P5236_05900"
     CDS             1429..2046
                     /gene="rplC"
                     /locus_tag="P5236_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRU63932.1"
                     /translation="MPGLIGKKIGMTSVFSEDGKNVPCTVIEAGPCFVTQVKTKEKDG
                     YEAVQLGFQTKNEKHTIKSEMGHFKAAGVLPQRHLVEFKDFEKELKPGDVLTVEIFEE
                     NSYVDIIGTSKGKGYQGVVKRHNFSGVGETTHGQHNRQRAPGSLGASSFPSRVFKGMK
                     MGGRMGGNRVTVQNLLVLKVIPEHNLLVVKGSIPGAKNSIVIINK"
BASE COUNT          773 a          280 c          423 g          619 t
ORIGIN      
        1 tgatactcta tgtgacgaaa agcatcctat tattttagag tcgattgatt tccctgaacc
       61 agtaattgga attagtgttg aacccaaaac tcagaaagat ttagatagat tgagtgtagg
      121 actttctaaa ttggctgaag aagatccaac ttttcaagta aaaacagatg aacaatcagg
      181 acagaccatt attagtggca tgggcgagtt acatctggaa attattttag atcatttaag
      241 gcgagaattt aaagtagaat gtaatcaagg gcgaccacaa gttgcttata aagaagctat
      301 tactaaacct gttgaaatca gagaagttta taagaaacaa acaggtggtc gtggtaaatt
      361 tgctgatata atagtaaggg ttgaacctgc agatgctgat tttgagggga gtttacagtt
      421 tattgattta gtgaaaggtg gaaatattcc aaaagaatat atacctgcta ttcaaaaagg
      481 ttttttatcg gctatgaaaa atggcgtttt ggcaggtttc cctgttgaca aattaaaagt
      541 aacagtttta gatggttctt atcatcctgt tgactctgat caattatctt ttgaaatttg
      601 tgctcaaata gcttttaaaa atgcttgtat taaagcaaat cctgtcttac tggaacctat
      661 tatgaagtta gaggtggtaa ccccggaaga aaatatggga gatgtgatag ctgatttgaa
      721 taagcgaaga ggacaaatag agaatacaga atccagccat acaggagctc gtattataaa
      781 agccaaagta ccattggcag aaatgtttgg atatgttacg gttttgcgta ctattacttc
      841 gggtagagca acttcttcta tggaatttga tcattatgaa gaagtgagca attctattgc
      901 aaaacaagta gtgggagaag caaaaggaaa agtagaatta ctaaattatt aatataatta
      961 attatgattc agattttttt tgttagcaaa tgactcttaa caaaaattat tctgaaattt
     1021 attaaaaata actatgagtc agaaaattag aattaaatta aaatcttacg attacaatct
     1081 ggttgataaa tcggctgaaa agattgtaaa aacagtgaaa gcgaccggag ctatagtaag
     1141 cggtccaata ccactgccca cgcataagcg tatatttaca gtgaatcgct ctacttttgt
     1201 caataaaaaa tcaagagagc agtttgagct ttcttcttac aaaagattga ttgatattta
     1261 tagctcaacg agtaaaacag ttgacgcatt aatgaaactt gaattgccaa gtggagtaga
     1321 agtggaaata aaagtttgat aaagtttttt aattcaatac ataaatataa atgtgtaatc
     1381 aatgaccgat cccacgcaag tggttaaggg tcttaaatta aatttaaaat gccaggatta
     1441 ataggtaaaa aaattgggat gacttctgtt ttcagtgaag atggaaaaaa tgttccttgt
     1501 acagtaattg aagccggtcc ttgttttgtt acacaagtaa aaacaaaaga aaaggatggc
     1561 tatgaagctg ttcagttagg ttttcaaaca aaaaatgaaa agcacaccat taaatcagaa
     1621 atgggacatt tcaaagctgc gggagtttta ccacaaagac atttggtaga atttaaagat
     1681 tttgaaaaag aattaaaacc aggcgatgtg cttacggtag aaatttttga agaaaatagt
     1741 tatgtcgata taattggtac ttcaaaaggt aaaggatatc aaggcgtagt aaaacgacat
     1801 aatttttctg gagttggtga aaccacgcat ggacagcaca atcgacaaag agcccccggt
     1861 tcattaggag cttcctcttt cccttctcgt gtatttaagg gaatgaaaat gggaggacga
     1921 atgggaggca atagagtaac tgttcaaaat ttacttgtat taaaagtgat acctgaacat
     1981 aatttattag tagtaaaagg ttcaatacct ggagctaaaa attcaatcgt aatcatcaat
     2041 aaataatcat ggaattaagt gtattaaata ttaagggaga agacacaggc cgaaa
//
LOCUS       DAOTKS010000298         2089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_42868_length_2089_cov_3.647493, whole genome shotgun sequence.
ACCESSION   DAOTKS010000298 DAOTKS010000000
VERSION     DAOTKS010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42868_length_2089_cov_3.647493"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1767)
                     /locus_tag="P5236_05905"
     CDS             complement(<1..1767)
                     /locus_tag="P5236_05905"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008025257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRU63933.1"
                     /translation="MKVLKFGGTSVGSVQGILNLKKIVESQNEQIVVVVSALSGVTDQ
                     LYHISKIAASGDESYLKEYDEMFRRHMEIIHQVISPEKKAKTVSQVQTLFADLLNIFR
                     GIYLIKDISTKITDTVVSYGEMISSVIIANVIENAVHFDSRTFIKTKDQFNKHMVDFK
                     ATNALIQEKFKDAPQIVVCGGFISTDVEENYVTNLGRGGSDYTSAILAAALNASILEI
                     WTDVDGFMTADPHIINNAYVIDRLSYSEAMELCNFGAKVIYPPTIFPTFHKSIPIIIK
                     NTFRPEVQGTYISKEKSGEPSKAIKGISSIDDTSLITIRGLGMVGVIGVNYRIFRALA
                     KNGISVFFVSQASSESTTSIGVRNADSALAVQVLKDEFIYELEQGEIDEIFSEDEMAT
                     IAIVGDNMKRTPGIAGKLFGTLGRNGINVVACAQGASETNISFVTDRSYLRKALNVIH
                     DSFFLSEYQVLNIFLVGIGMVGSNLLNQIKMQQPKLIQEKGLKLNVVGIATSEKMYFD
                     REGIDIEQYNEILETKGVVSSSEKIASEIINMNIFNSVFVDCTASEQIADLYQQLLDH
                     NISVVAANKIAASSSYDKYIRLK"
     gene            2014..2087
                     /locus_tag="P5236_05910"
     tRNA            2014..2087
                     /locus_tag="P5236_05910"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2048..2050,aa:Arg,seq:tct)
BASE COUNT          707 a          400 c          296 g          686 t
ORIGIN      
        1 tttcaaccga atgtatttgt cgtacgaaga agaggctgct attttattag ctgctaccac
       61 agaaatatta tgatcgagta attgttgata caaatcagca atttgttcac tggcagtaca
      121 atccacaaaa acagaattaa aaatgttcat attgataatt tcactagcaa tcttttcaga
      181 ggaagatact acaccttttg tttccaaaat ctcattatat tgttcaatat caattccttc
      241 tctgtcgaaa tacatttttt cacttgtagc tattcctaca acattcaatt ttaatccttt
      301 ttcctgtatc aatttgggtt gctgcatctt gatttgattt aacaaattgc ttcccaccat
      361 accaataccc accaaaaaaa tattcagcac ttgatactca gacagaaaaa acgaatcatg
      421 aataacattc aatgcctttc gcaaatagct tctatcagta acaaatgaaa tatttgtttc
      481 cgatgccccc tgtgcacaag caacgacatt aataccattt cgtcctaaag taccaaaaag
      541 ttttccagct ataccagggg tacgtttcat attgtcgcca acaatggcaa ttgttgccat
      601 ctcatcttca gaaaaaatct catcaatttc accctgttct aattcataaa taaattcatc
      661 tttcagcact tgaacagcta aagcactatc agcatttcga acaccaatgg aagtagtact
      721 ttcagaagaa gcctgcgaaa caaaaaatac gctgatgcca ttttttgcca aagcacgaaa
      781 aattcggtaa ttaactccaa taacacccac cattcccaaa cctcgaatgg taattaagga
      841 agtatcgtcg atggatgaaa ttcctttaat ggctttggaa ggttcacccg acttttcttt
      901 ggaaatatat gtaccttgta cctcaggacg aaaggtgttt ttaataataa ttggaatact
      961 tttatgaaaa gtaggaaaaa tagtaggagg ataaatcact tttgcaccaa aattacaaag
     1021 ttccattgct tcactataac tcaatcgatc aattacataa gcgttattaa tgatatgtgg
     1081 atcggcagtc ataaagccat caacatctgt ccagatttcg agaatggaag catttaaggc
     1141 agcagcaaga atagcagaag tataatcaga acctcctcta cccaaattag taacataatt
     1201 ttcttctaca tcggttgaaa taaatccgcc acatactaca atctgaggag catccttaaa
     1261 tttttcttga ataagagcat tggtagcttt aaaatctacc atgtgtttat taaactgatc
     1321 ctttgtttta ataaatgttc gtgaatcaaa atgaacagca ttttctatta cattggcaat
     1381 aatgacagaa gaaatcattt caccataact tacaacagta tcagtaattt tagtggatat
     1441 atctttaatt aaataaattc cacgaaatat atttaaaaga tcagcaaaca atgtctgaac
     1501 ttgactgact gtttttgctt ttttttcagg agaaatcact tgatgaatga tttccatgtg
     1561 acggcgaaac atttcatcat attcctttaa gtaactttca tcaccactcg cagctatttt
     1621 tgaaatatga tataactgat ctgttacacc tgacaaagca gaaaccacta ctactatctg
     1681 ttcgttttgt gattcaacaa ttttttttaa attcaaaatg ccctgcactg agccaaccga
     1741 agtgccgcca aattttaata ctttcattta tcagtctata tatttattat tttgataata
     1801 atacctttta attttacaaa attagttatt tttagtcgaa aaagactcct tattatgaat
     1861 tttgaataaa aacacttgcg ttttttttaa aattacagta cttttgcaac cggaaatggc
     1921 aggaaaatca atcgaaaata ataaaaattg cattcttaat gattcattga tactgattaa
     1981 ttttgttttt ctttttttac accaaaaaat tctggtctcg tagctcagtt ggatagagca
     2041 acagccttct aagctgtggg tcgtgggttc gaatcccgcc gagatcacc
//
LOCUS       DAOTKS010000299         2079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_43165_length_2079_cov_3.411067, whole genome shotgun sequence.
ACCESSION   DAOTKS010000299 DAOTKS010000000
VERSION     DAOTKS010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43165_length_2079_cov_3.411067"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1606)
                     /locus_tag="P5236_05915"
     CDS             complement(<1..1606)
                     /locus_tag="P5236_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRU63934.1"
                     /translation="MRKITLFMAALAFSFYAMADGMSGTYKVGTGETAPNFTLLSEAV
                     TAINTNGLTGDVILEITSDITETVNIGLINNSDFTITIRPDQDADRTITFDKATDNDG
                     PSGAFCLGIGMGLKWADLSPTKNVIIDGYAIGGNTRRLKITTAVTHNGLDGPILLMDD
                     CSNIQIKNLIIHHVGTSTGSSSNYGIYLRVNTSYGTKKMPSNVLIENNEITAIQNTAS
                     QGIGIYANAAPASLATGVMIKNNIIKAKTRGIFLYYTNDLNIINNEFRINQPSDGMLS
                     TAIMGNNGQTGNINVIGNKFIELKTGNSTAGAYGMRAIIASGGGTWNIINNFFTGFDK
                     TSTKAGETMLQGIRCGSTCIILHNTFYLNELTNKPTYVATPTETQGCYCAINIAAGTP
                     LIKNNIFVSNEDAVYNFATRGTVTVGYSDYNIFDAHAGTTYTGVNSVYPDFSQYQASG
                     VDINSKSISVTLTDPANGDLRLTGASDGDANLGVPRLAEVTTDMFGTSRPSITYAGAH
                     EATPLVTKTFTVTVPNGTEHVYIAGSF"
     gene            complement(1803..>2079)
                     /locus_tag="P5236_05920"
     CDS             complement(1803..>2079)
                     /locus_tag="P5236_05920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRU63935.1"
                     /translation="KLDVISEVPQLEKEKTEILLYPNPVNNYLKIESKNETLKMVTIY
                     DLRGSMVRSERISGISAEISTDNLPAGNYIIKVQTENRVITKRIIKQ"
BASE COUNT          674 a          372 c          317 g          716 t
ORIGIN      
        1 taaacgaacc cgcaatataa acatgttcag ttccattggg aacagttaca gtgaaggtct
       61 tggttaccaa aggagttgct tcatgagcac cagcataagt aatagaagga cgggatgtac
      121 caaacatatc tgtggtaact tcagccagac gaggaacacc taaatttgca tctccatccg
      181 aagcaccagt aagtcgtaaa tcaccattgg ctggatcagt caaagtaaca gaaatacttt
      241 tagaatttat atcaacacct gaggcttgat attgagaaaa atctggataa acagaattta
      301 caccagtgta agttgtacct gcgtgagcat caaaaatatt ataatcagaa taacctactg
      361 ttacagtacc tcttgtagca aaattataaa ctgcatcttc attgctaaca aaaatattat
      421 ttttaattaa aggagtgcca gcagcaatat tgatagcaca ataacatcct tgagtttctg
      481 ttggtgtagc tacataagtg ggtttatttg tcaattcatt taggtaaaat gtattgtgta
      541 agataataca tgttgatcca catctaatac cttgaagcat cgtttcgcct gcttttgttg
      601 aagttttatc gaaacccgta aaaaaattat tgataatatt ccaagttcct cccccacttg
      661 cgataatagc tctcatacca taggcaccag cggttgaatt tcctgtttta agctcaataa
      721 acttattccc tattacattt atattgccag tttgaccatt gttacccata attgcagtac
      781 ttagcattcc atctgaaggt tgatttattc gaaattcatt atttatgata ttaagatcat
      841 ttgtataata taaaaatatt cccctcgttt tggctttaat aatattattt tttatcataa
      901 cacctgtagc aagcgaagca ggagcagcgt tagcataaat acctattcct tgggaggcag
      961 tattttgaat ggctgtgatt tcattgtttt caattagaac attagatggc atttttttag
     1021 taccatagct cgtgtttact ctaagataaa taccataatt actactactt cctgttgatg
     1081 taccaacgtg atgaataatt aaatttttaa tttggatatt tgagcaatca tccattaata
     1141 aaataggtcc atccaaacca ttatgtgtta cagcggtggt aatctttaac ctccgagtat
     1201 ttcctccaat agcgtaacca tctattatta catttttagt aggtgataaa tcagcccatt
     1261 ttaatcccat cccaatgccg agacaaaaag cccctgatgg tccatcatta tctgtagctt
     1321 tgtcaaaggt aatagttctg tccgcatctt gatccggtct tatagttatt gtaaaatctg
     1381 aattgttaat taagccaatg tttactgttt cagtaatatc tgatgtaatt tcaagaataa
     1441 catcgcctgt taagccattg gtattaattg cagttactgc ttcacttagt aaagtaaaat
     1501 tcggagcagt ttctcctgta cctaccttgt aagtaccaga cattccatcg gccatggcat
     1561 aaaaactgaa tgccagtgcg gccataaata acgtaatttt tctcatgata aaatagtttt
     1621 aaaaagtaaa taaaaaaata aaattattta acgcgataaa tataaaatta attttgtaaa
     1681 actgcaaacg tttgctaaat attttttacg tttttaaaaa ttattactaa aatagtttat
     1741 ttagcaaaaa atccaaaaga cagcaatgcc tgtaaacact gctgtcttta agatatagaa
     1801 atttattgtt tgatgattcg tttggttatt actctattct ctgtctgcac tttaataata
     1861 taatttcctg caggcaaatt gtcagtagaa atttcagcac ttatgccact tattctttct
     1921 gaacgcacca tgctacctct caaatcatat atggtaacca tttttaaagt ctcattttta
     1981 ctttcaattt ttaaatagtt gttcacagga tttggataca gtaaaatttc tgttttttcc
     2041 ttttcaagct gcggaacctc actgattaca tctaatttt
//
LOCUS       DAOTKS010000300         2074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_43321_length_2074_cov_2.475978, whole genome shotgun sequence.
ACCESSION   DAOTKS010000300 DAOTKS010000000
VERSION     DAOTKS010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43321_length_2074_cov_2.475978"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(631..1632)
                     /gene="gap"
                     /locus_tag="P5236_05925"
     CDS             complement(631..1632)
                     /gene="gap"
                     /locus_tag="P5236_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRU63936.1"
                     /translation="MIKVGINGFGRIGRLVFRAAQERNDIQVVAVNDPFLDMDYLIYM
                     LKFDSVHGKFEGTAEKKDGKLFVNGKEVVFFAEKDPVNIGWAATGAEYIVESTGVFTT
                     MEKASAHLKAGAKKVVITAPSNDAPMFVCGVNLDKYTPDMNIVSNASCTTNCLAPLAK
                     VIHEKFGIVEGLMTTIHAATNSQKTVDAPSNKDWRGGRSILNNIIPSSTGAAKAVGKV
                     IPSLNGKLTGMSMRVPVADVSVVDLTCRLEKAATYEEIKAAVKAASENELKGILGYTE
                     DAVVSTDFIHDPRTSIFDANAGIALNSNFVKLISWYDNEWGYSNKVLDLIQHMASVK"
     gene            complement(1842..>2074)
                     /locus_tag="P5236_05930"
     CDS             complement(1842..>2074)
                     /locus_tag="P5236_05930"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HRU63937.1"
                     /translation="MKTNVGGNDKILRLGVAIVLIILFYKSILKGTLGLIGLIIAFLL
                     TLSSLIGFSFLYSIFKIDTSKKKEKEKRKPEK"
BASE COUNT          724 a          353 c          285 g          712 t
ORIGIN      
        1 tgctctttcc aaaaatgaac agaacaaaga taaaaaaaat cattattcta tgccaacttg
       61 tttttcaaac atgaaaataa tttagtattt agatcatctt agaattatat cttgaatgta
      121 agcagtccag agaaaaacac tcaagaaatg ctccatgaaa aatcagtttg aaagtcgatt
      181 ttttccagga aatagaaaaa taagatatgt gtatccgcga agcatcctaa agagatttga
      241 caacaggaaa aagttataaa aaattccaaa aaaattattg tataattcat tgatatttaa
      301 tatctttata tcataattta tcattaaagg aacaacaaaa tgaatatctt atataaagtt
      361 taaaaattga ttgcacaaat ggttaccctt ttttaaattg agattaggaa atgtcataat
      421 attttgaatg gttttattcc ttttaaaaaa ggtttgtgta tttacaaatt agaatctatt
      481 tattatattt aggtatttag caaaatgtct ctactcgaaa cttgcttccc tcacctcgaa
      541 tctcgaacct cgcataatgg tatgaaacaa aaaaactccc ttcaaatatt taattttcag
      601 ggagtttttg gctaagttta tgaaaaaaaa ttattttaca gaagccatgt gttgaattaa
      661 atcaagcact ttgtttgaat aaccccattc attgtcgtac caactaatca attttacgaa
      721 attactattt agggcaattc cagcattggc atcaaaaata gaggtacgcg gatcgtgaat
      781 gaaatcagtg gatacaaccg catcttccgt atagcccaaa attcctttta attcattttc
      841 agaagcagct tttacagcag cttttatttc ttcataagta gcggcttttt ctaaccggca
      901 agtcaaatca accactgata catcagctac tggaacacgc attgacattc ctgtcaattt
      961 gccattcaat gaaggaataa ctttacctac ggcttttgca gcaccagttg aagagggaat
     1021 aatattgttc aagatagaac gaccgcctct ccaatcttta tttgaaggag catcaactgt
     1081 tttctgagaa ttggttgctg catgaatagt ggtcattaat ccttcaacga tgccaaattt
     1141 ttcatgaatt acttttgcta atggagcaag acagttagtg gtacacgaag catttgaaac
     1201 aatgttcata tcaggagtgt atttatccag attaacaccg caaacgaaca ttggagcatc
     1261 attggaagga gctgtaatga ctactttttt tgcacctgct tttaaatgag cagaagcttt
     1321 ttccattgtg gtgaatactc ctgtagattc aacaatgtat tcagcaccag tagcagccca
     1381 accgatattt acagggtctt tttcagcaaa gaaaactact tctttaccat taacgaaaag
     1441 tttaccatct tttttttcgg cggtaccttc aaatttacca tgtacactat caaatttcaa
     1501 catataaatc agataatcca tatccaagaa tggatcattc actgcaacga cttgaatgtc
     1561 gtttctttct tgagcagcac ggaataccaa acgtccaatg cggccaaaac cattaatacc
     1621 tactttaatc attttattac ttatttttaa aattagttta ctttaaatct gtgcaaaatt
     1681 acagaattat gtttgattct caaaacactt caaattataa ttaaagaaat ttttatgtaa
     1741 ttaaatctta cattttaatt tatttcaata aaatttaatc actatattct tttctctcac
     1801 tttttattgt agaaaaaatg agtatttatt ttttcttact tttatttttc tggttttctt
     1861 ttttcttttt cctttttctt tgaagtatcg attttaaaaa tagaatatag aaaagaaaaa
     1921 ccaattaagc tggaaagtgt taataagaaa gcaattatca agccaatcag acctaacgtt
     1981 ccttttaata tacttttata aaaaagtata atcaatacga tagccacgcc caatcgcaaa
     2041 attttatcat taccaccaac atttgttttc atgg
//
LOCUS       DAOTKS010000301         2064 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_43617_length_2064_cov_3.646093, whole genome shotgun sequence.
ACCESSION   DAOTKS010000301 DAOTKS010000000
VERSION     DAOTKS010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43617_length_2064_cov_3.646093"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2064)
                     /locus_tag="P5236_05935"
     CDS             complement(<1..>2064)
                     /locus_tag="P5236_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HRU63938.1"
                     /translation="RERQSTGVFDTWANFAMPTYMGSASWDWMPYVPGLNCGITGNVY
                     LSTTGMVKLDDVWVRSDLPDDSHANLSIQATINNTCNQSVTGILNGTIMPGNIHFSKT
                     VVIEANQSLLVKINQEEYAQLIINNPLLWWPIGYGNQNLYTLSLEFKVNNEVSDAQAV
                     IFGIRKYEYKNENGAFTFYVNHSKVFVRGGNWGVSEYLLRCKGEDYDTRLKLHADMNF
                     NMIRCWTGCVTDDEFYDYCDKYGIMVWDDFWLYVAFNDVAQPEAFKANALDKVRRLRN
                     HPCIAVWCGANETHPKPELDNYLREMIAKEDNNDRLYKSCSNQDGLSGSGWWGNQPPK
                     HHFETSGSNLAWNDPPYPYGSDYGYGFRSEIGTATFPVYESIIKFMPQDQLWPLPTDE
                     QLRNDDNTIYNKHFFGKEASNANPVNYKRSVNTEFGESANLQEFCEKAQLLNIEVMKG
                     MYEAWNDKMWNDASGLLIWMSQSAYPSFVWQTYDYYYDATGSYWGAKKACEPQHIQWN
                     CSSNSVKVINTTSKALTNVKAMVNVYDINGKEIGKYRKSAVLNVNPSNINEAFVINFN
                     TSNLALGKKAFASSSTKNMDAALITDGASGSRWESEYNDPQWIYVDLGKKQKIESVVL
                     KWEAAHAKEYELQVSNDANNWTTIYKQQDSKGGTETIKIKPVSAQFVKLNCISRATIF
                     GYSLF"
BASE COUNT          587 a          447 c          345 g          685 t
ORIGIN      
        1 tcgaatagtg aatatccgaa aatggttgct ctcgaaatgc agtttagttt gacaaattgt
       61 gccgatacag gtttaatttt tattgtttca gttccacctt tggaatcttg ttgcttataa
      121 atagttgtcc aattatttgc atcgtttgat acttgcaatt catattcttt ggcatgagct
      181 gcttcccatt ttaataccac agattcaatc ttttgttttt tccctaaatc cacataaatc
      241 cattgcggat cgttatattc gctttcccaa cgactgccac ttgcaccatc tgtgattaaa
      301 gcagcatcca tattttttgt tgacgaggaa gcaaatgctt ttttacccag tgcaagattg
      361 gaagtattga aatttataac aaaagcttcg ttaatattgc tgggatttac atttaaaact
      421 gcagatttcc tgtattttcc gatttctttt ccatttatat cgtaaacatt taccattgct
      481 tttacgtttg tcagggcttt ggaagtagta tttataactt ttacgctatt gctcgagcaa
      541 ttccattgaa tgtgttgcgg ttcgcaagct ttcttggctc cccagtaaga gcctgtagca
      601 tcataatagt aatcgtaggt ttgccataca aacgaaggat aggcagactg gctcatccaa
      661 atcagtaatc cggaagcatc attccacatt ttgtcgttcc aagcttcata cattcctttc
      721 attacctcaa tgttgaggag ttgagccttt tcacaaaatt cctgcaaatt tgcagattct
      781 ccaaattcag tattgaccga tcttttgtaa ttaaccggat tggcatttga agcttctttc
      841 ccaaaaaaat gcttgttata aatcgtattg tcgtcatttc ttagctgttc atccgttggc
      901 aaaggccaca actgatcttg aggcataaat ttgatgatac tttcataaac aggaaaagtt
      961 gccgttccta tttcgctacg aaaaccgtaa ccataatccg aaccatacgg atacggagga
     1021 tcattccatg ccagattgct gccggaagtt tcaaaatgat gttttggcgg ttgatttccc
     1081 caccagccac tacccgataa tccatcctga ttggaacacg atttatatag acgatcgtta
     1141 ttatcttcct tagctatcat ttccctgaga taattatcca actcgggttt cggatgcgtt
     1201 tcatttgctc cgcaccacac tgcaatacac ggatgatttc tcaacctacg aactttatcg
     1261 agggcgttgg ctttgaaagc ttccggttga gccacatcat taaaagccac atacaaccaa
     1321 aagtcatccc acaccattat gccgtacttg tcgcaataat cgtaaaactc gtcatctgtt
     1381 acgcatcctg tccagcaacg aatcatgtta aaattcatat cggcatgaag tttcagtcga
     1441 gtatcgtaat cttccccctt gcagcgtaac agatattcgg acactcccca atttcctcct
     1501 ctgacaaaaa ctttggaatg gtttacataa aatgtgaaag ctccattctc gtttttatat
     1561 tcatattttc tgatgccaaa aataacagcc tgagcatctg aaacctcatt gttaactttg
     1621 aattctaaac ttaacgtata aagattttga ttaccataac ctatgggcca ccaaagaaga
     1681 ggattattta taattaattg agcatattct tcctggttta tttttaccaa taaagattga
     1741 tttgcttcaa taaccaccgt ttttgaaaaa tgaatattac ctggcatgat agttccgttt
     1801 aatattccag ttacggattg attacaggta ttattaatgg tagcttgaat acttaaattg
     1861 gcgtgactgt catcaggaag gtctgaacgc acccaaacat catccaattt caccatgcca
     1921 gtagttgaaa gataaacatt gcctgtaatt ccacaattta aaccaggaac atatggcatc
     1981 cagtcccagc tggcacttcc catataagtg ggcatagcaa aatttgccca tgtgtcaaat
     2041 actcccgttg actgcctttc tctt
//
LOCUS       DAOTKS010000302         2059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_43775_length_2059_cov_2.933633, whole genome shotgun sequence.
ACCESSION   DAOTKS010000302 DAOTKS010000000
VERSION     DAOTKS010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43775_length_2059_cov_2.933633"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1788)
                     /gene="mnmG"
                     /locus_tag="P5236_05940"
     CDS             complement(<1..1788)
                     /gene="mnmG"
                     /locus_tag="P5236_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRU63939.1"
                     /translation="MDFKYDIVVVGSGHAGNEAACAAANMGSKTLLITMDMNKIGQMS
                     CNPAVGGIAKGQIVREIDALGGQMGIVADRSAIQFRMLNRSKGPAMWSPRSQSDRHQF
                     IREWIKVISNTPNLYLWQDTVKELIIEDDTVKGVITALGVKISAKCVILASGTFLNGL
                     IHVGKTQMRGGRISEPASLGLTEQLVSSGFEAGRMKTGTPARIDGRTIDFSQLKEQVG
                     DNDFHKFSFLEEIHRELKQMSCWITYTNSITHEKLRKGLPDSPLYNGQIKSIGPRYCP
                     SIETKIITFAEKQSHQIFLEPEGVDSQEYYVNGFSSSLPYPIQIKALHTIAGLENAQL
                     YRPGYAIEYDFFDPTQLKHTLETKKIKNLFFAGQINGTTGYEEAAGQGLIAGINAHMR
                     CHGDAEFTLARDESYIGVLIDDLVTKGVDEPYRMFTSRAEHRLLLRQDDADARLTEKG
                     YQIGLASQNRYYQYQRKKEEQTKLIQFIQNYSVKPQEVNAYLSQKGLSELKHGCKLSD
                     LTLRPQLNINDLVSVIPALKQKIDEIPSRREEIIESIEIILKYSGYIEREKTIAEKLQ
                     RLEHLNIRGRFDYDSILSLSTEARQKLMRV"
     gene            complement(1866..>2059)
                     /locus_tag="P5236_05945"
     CDS             complement(1866..>2059)
                     /locus_tag="P5236_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRU63940.1"
                     /translation="GHIICNQLSVAEIVVPIFDANQNIVGVLDIDSDKFDELTEVDVF
                     YLEKICQLITNKLSNFFHV"
BASE COUNT          654 a          383 c          320 g          702 t
ORIGIN      
        1 gactcgcatc agtttttgcc gagcttcggt tgataaagat aagatagaat cataatcaaa
       61 acgtccacga atgtttaaat gttctaatcg ttgaagcttt tctgcaatcg ttttttcacg
      121 ttctatgtag ccactgtatt tcaaaataat ttcaatagat tcaataattt cttctctccg
      181 tgagggaatc tcatcaattt tttgttttaa agcagggata acagaaacca aatcattaat
      241 gtttagttga ggtcttaaag ttaaatcaga taatttacaa ccatgtttta attctgataa
      301 acctttttgt gaaagataag catttacttc ttgaggtttt actgaataat tttgaataaa
      361 ttgtataagt tttgtttgtt cttctttttt tctttgatac tgataatagc gattttgtga
      421 agccaatcct atttgataac ctttttcagt taaacgagcg tcagcatcat cctgacgaag
      481 aagtaaacga tgttcagctc gtgaagtgaa catacgataa ggttcatcta ctccttttgt
      541 aactaaatca tcaattaata ctcctatata ggattcatca cgagccaaag taaattcagc
      601 atcgccatga catctcatgt gagcgttgat gcctgcaatc aaaccttgac ctgctgcttc
      661 ttcatatcca gttgttccat taatttgccc ggcaaaaaaa agatttttga ttttctttgt
      721 ttccagtgta tgttttaatt gtgtcggatc aaaaaaatca tattcaatgg cataacccgg
      781 tctgtaaagt tgagcgtttt caagaccagc aatggtatga agagctttaa tttgaatggg
      841 atacggaagt gaagaagaaa atccattcac ataatactct tgagaatcca caccttcagg
      901 ttcaagaaaa atttggtgac tctgcttttc agcaaatgta ataattttag tttcaatgct
      961 cggacaataa cggggaccaa tacttttaat ctgtccattg taaagtggag aatccggtaa
     1021 tcctttacgt aatttttcat gagttatgga attggtataa gtaatccagc aactcatttg
     1081 ttttaattca cgatgtattt cttctaaaaa ggaaaattta tgaaaatcat tatcgcctac
     1141 ttgttcttta agttgagaaa aatcaatggt acgaccatca attctggcag gtgttcctgt
     1201 tttcattctt ccagcttcga aacccgaaga aacaagttgc tctgtcaaac caagtgaggc
     1261 aggttctgaa attcgtcctc ctctcatttg ggtttttccg acatgaatta atccgtttaa
     1321 aaaagttcca gaagcaagaa ttacacattt agcagatatt ttcactccca aagcagtaat
     1381 aacacctttt accgtgtcat cttcaattat taattccttt accgtgtctt gccaaagata
     1441 taaattaggt gtattactaa ttacttttat ccattcacga ataaattgat gacgatcgct
     1501 ttgtgaacgt gggctccaca tagcaggacc tttggaacga tttaacatcc ggaattgaat
     1561 tgctgaacga tctgcaacaa ttcccatttg accgcccaat gcatcaattt cacgaacaat
     1621 ctgtcctttg gcaataccgc ctactgcagg attacagctc atctgaccga ttttgttcat
     1681 gtccattgta atcagcaaag tctttgaacc catattggct gcagcacagg ctgcttcatt
     1741 gcctgcgtgt ccactcccaa caactactat atcgtattta aagtccattt atctcaaaaa
     1801 attaaaaaat atcataacaa ccaatatgaa tttttagtaa aaaattgttt cttgttaata
     1861 tttttttaaa catgaaaaaa attagataat ttattcgtaa tcaattgaca aattttttct
     1921 aaataaaata catctacctc tgtcaattca tcaaatttat cactgtcaat atctaaaaca
     1981 ccaacaatat tttgattagc atcaaatatt ggaacaacaa tttctgcgac tgaaagttga
     2041 ttgcagataa tatgtccag
//
LOCUS       DAOTKS010000303         2054 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_43895_length_2054_cov_5.197099, whole genome shotgun sequence.
ACCESSION   DAOTKS010000303 DAOTKS010000000
VERSION     DAOTKS010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43895_length_2054_cov_5.197099"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..>2054)
                     /gene="carB"
                     /locus_tag="P5236_05950"
     CDS             complement(165..>2054)
                     /gene="carB"
                     /locus_tag="P5236_05950"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRU63941.1"
                     /translation="ELTKIDRWFLYKLKHIIDLEKELMGYNSLSSLPDKLLYEAKKAG
                     FSDFQITRLVLKCSSDEMDDMIKVTREYRKSRNILPVVKQIDTLAAEYPAQTNYLYLT
                     YNGATHDIQYENDQLSVIVLGSGAYRIGSSVEFDWCSVNALMTIKKEGYRSVMINYNP
                     ETVSTDYDMCDRLYFDELTFERVMDIIELENPKGVIVSTGGQIPNNLALKLAKANVKI
                     LGTQAEFIDMAEDRHKFSAMLDQLGIDQPRWKELTTFEDVKEFVNRIGYPVLVRPSYV
                     LSGAAMNVCYDDSQLENFLKVATEVSKKHPVVVSEFLERCKEIEIDAVANKGEIIAYA
                     ISEHIEFAGVHSGDATTQFPPQKIYVETIRRIKNIARQIAHSLQISGPFNIQFLARDN
                     YIKVIECNLRSSRSFPFVSKVLKINFIDLATRIMLGLPVEKPEKSAFDLDYVGIKASQ
                     FSFARLQQADPILGVDMASTGEVGCLGDDFSEAVLKALLSVGFKIPEKNILISSGEPK
                     SKVDLLDACRLLYKKGYQIYATRGSHQFLAENGIESKVVLWPDEEGEYNVKDMIAQRK
                     FDLVINIPKNVTQRELANDYVIRRSAIDYNIPLLTNARLASAFIFAFCNLKLEDIKIK
                     SWDEY"
BASE COUNT          684 a          405 c          293 g          672 t
ORIGIN      
        1 cagacgcgta ctctaaaccg gactgagcta catcccgatt atttgaaaag aatacaaaaa
       61 taaccatttt tttattttaa acgaaatcta tttttttgtt tatccagatt tcctcaaaaa
      121 caataattca ttgattggta ttggtggaaa ttatttttca aaaattaata ttcatcccag
      181 ctttttattt taatatcttc caatttcaaa ttacaaaaag caaaaataaa agcactggca
      241 agtcgtgcat ttgttaataa aggaatatta taatcaatag cactgcgacg tatgacataa
      301 tcatttgcca attcacgttg agttacattc tttggaatat taattaccaa atcgaatttc
      361 ctttgtgcaa tcatatcctt gacattatat tcgccctctt catcaggcca taggactact
      421 ttactttcaa tgccattttc agctaaaaat tgatggcttc ctcttgtagc ataaatctga
      481 taaccttttt tatacagtaa cctgcaagca tcaagcaaat ccacttttga ttttggttca
      541 cctgaagaaa tcagaatatt tttctccgga attttaaaac caactgataa caaagctttt
      601 aaaactgctt ctgaaaaatc atcacctaag cagccaactt ctcccgttga agccatatct
      661 actcctaaaa tggggtcagc ttgctgcaaa cgtgcaaatg aaaattgaga agctttaatg
      721 cccacataat ctaaatcgaa agctgatttt tcaggttttt ccacaggaag tcccagcata
      781 attctggttg ctaaatcaat gaaatttatt tttaacactt tcgaaacgaa aggaaaactt
      841 cgtgaagaac gcaaattaca ttcaattact tttatataat tatcacgcgc caaaaactga
      901 atgttaaatg gtcctgaaat ttgcaaggaa tgagcaattt gtcgagcgat atttttaatc
      961 ctgcgaatgg tttctacata aattttttgt ggtggaaatt gagtggtggc atcacctgaa
     1021 tgtacaccag caaattctat atgttctgaa atagcgtaag caataatttc acccttattg
     1081 gcaactgcat cgatttctat ttccttgcaa cgttccaaaa attctgaaac tactactgga
     1141 tgctttttag aaacttccgt tgcaactttt aagaaatttt caagttgcga atcatcataa
     1201 caaacattca tagcagcacc cgataataca taagaaggac gaaccaatac ggggtatcca
     1261 attcgattca caaattcttt aacatcttca aaagtagtca attctttcca tcgtggctga
     1321 tcaattccca attgatcaag catagcagaa aatttatgac gatcttctgc catatctata
     1381 aattcagctt gcgtacctaa tatttttaca tttgccttag ctaatttcaa agccagattg
     1441 ttaggtattt gcccgccagt tgaaacaatt acaccttttg gattttccag ctcaataata
     1501 tccattaccc gttcaaaagt caattcatca aaatacaatc gatcgcacat gtcataatca
     1561 gttgagacgg tttcaggatt atagttgatc atcacagaac ggtaaccttc ttttttgata
     1621 gtcatcagag catttacact acaccaatca aattccacag aagaaccgat tcggtaagct
     1681 cctgaaccaa gaactataac tgaaagctgg tcgttttcat actgaatatc atgagtagct
     1741 ccattatatg ttaaatacaa gtagttagtt tgtgcaggat attcagccgc cagcgtatca
     1801 atttgtttga caacaggtaa aatatttcga cttttccgat attcacgcgt cacttttatc
     1861 atatcatcca tttcatcgct actgcattta agaacaagcc gtgtaatctg aaaatcagaa
     1921 aaaccagctt ttttagcttc atacaataac ttatcaggca gagatgataa agaattataa
     1981 cccattaatt ccttttccaa atcaataata tgttttaatt tgtaaagaaa ccatcggtct
     2041 atttttgtta actc
//
LOCUS       DAOTKS010000304         2054 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_43898_length_2054_cov_3.463732, whole genome shotgun sequence.
ACCESSION   DAOTKS010000304 DAOTKS010000000
VERSION     DAOTKS010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43898_length_2054_cov_3.463732"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="P5236_05955"
     CDS             <1..387
                     /locus_tag="P5236_05955"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRU63942.1"
                     /translation="VAWIIYGMVSVGGYLEIYFFATIFVLAFSGLGLVISNYANTIQQ
                     AMYLMFFFVINFIFLSGLYTPISSMPQWTQVMSFFSPLRYMIEALRSIYLKGSSFIDL
                     WHQFVALIGFAGLFNLWAVYSYRKSA"
     gene            437..1357
                     /locus_tag="P5236_05960"
     CDS             437..1357
                     /locus_tag="P5236_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HRU63943.1"
                     /translation="MENLNSKVQSLFQQQISEWDLARKNYGSLKNVQVKTFSFGDFDI
                     IVQFNPARIVSSGAKVDEKTIKERKCFLCLENLPPQQRGVKAGDYTILVNPYPIFPEH
                     FTIPRQEHIPQQIKPFFCDMLELAKSLDEFIVFYNGPKCGASAPDHMHFQAGTKDFLP
                     LINDYRRLKSTHTELLEKGNHYCLYYFKNYLRTVYCFEADDSEAAQYQFSKIYDNLQK
                     CSQLQVGEEPMINLICLFENNQWTVFIFPRQAFRPWQYSAEEDKRLLVSPATVEMSGI
                     FITPVEEHFKKITKEDIVNILEQLSMNKIV"
     gene            1398..>2054
                     /locus_tag="P5236_05965"
     CDS             1398..>2054
                     /locus_tag="P5236_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HRU63944.1"
                     /translation="MITTEPIIHVGILSSKKIEFIFHGFFVTAEGEKYSGKQKAEWKN
                     GKIFFDGKTSDELFFQPIDESNTFDLLDVVIGIHFHWERKENQRFKGSLRIIIEKDQL
                     TAINDIKAEDYLVSVISSEMSATSSLELLKAHAVISRSWLFYPLQNDKKNFFPQNSVE
                     NDTENRHIKWYERGEHEHFDVCADDHCQRYQGITRATSETVQLAIEATRGEVLMFDHQ
                     I"
BASE COUNT          658 a          330 c          398 g          668 t
ORIGIN      
        1 gtggcgtgga ttatttacgg aatggtttcg gtgggcggct atttggaaat ctattttttt
       61 gccactattt ttgtgcttgc tttttctgga ttggggctgg tgatttccaa ttatgccaat
      121 actattcagc aggccatgta tctgatgttt ttctttgtga taaattttat ttttctcagc
      181 ggactttaca cacctatttc cagtatgcca cagtggacgc aggtaatgag ttttttcagt
      241 ccgctgcgtt acatgatcga agcacttcgt tctatttacc tcaaaggcag cagttttata
      301 gatttgtggc atcaatttgt ggcattgatt ggttttgctg gattgtttaa tctttgggct
      361 gtttatagtt atagaaaaag tgcttgatcc tttgattttt agaacagaaa caaaattttt
      421 aaagcaaaaa tactggatgg aaaatttaaa ttcaaaagtt cagagtttat ttcaacaaca
      481 aatttctgaa tgggatttgg caagaaaaaa ttacggaagc ttgaaaaatg ttcaagtcaa
      541 aaccttttcg tttggtgatt ttgacatcat tgtgcagttt aatcctgcac gaattgtttc
      601 gtccggtgca aaagtggatg aaaagaccat aaaggaaaga aaatgttttt tgtgcttgga
      661 aaatttacca ccgcaacaaa gaggtgtaaa agctggcgat tatacaatat tagtaaatcc
      721 ttatcctatc tttcctgaac atttcaccat tccccgccaa gaacatattc cacaacaaat
      781 aaagccattt ttttgcgata tgctggagct ggcaaagtcg cttgacgaat ttatcgtttt
      841 ttacaatggt cccaagtgtg gcgcttctgc tcctgatcac atgcattttc aagcagggac
      901 aaaagatttt ttacctttga taaatgatta tagacgatta aaatctactc atacagaatt
      961 attggaaaaa ggaaatcatt attgcctgta ttactttaaa aattatcttc gtaccgtcta
     1021 ttgttttgaa gcagacgaca gcgaagcagc ccaatatcaa ttcagtaaga tatatgataa
     1081 tttgcagaaa tgtagccagt tgcaagttgg tgaagaaccc atgataaacc tcatttgctt
     1141 atttgaaaat aatcaatgga ctgtttttat ttttccgcgt caagcattca ggccctggca
     1201 atattcagca gaagaagaca aacgtttact ggtaagtcca gccacagtgg agatgagcgg
     1261 tatttttatt acacccgttg aagaacattt taaaaaaatt acgaaagaag acattgttaa
     1321 cattcttgaa cagcttagta tgaataaaat tgtttgattt ttagtaactt attgttaaat
     1381 ataaccattt aattattatg ataacgactg aacccatcat ccatgttgga atattaagtt
     1441 ccaaaaaaat agaatttatc ttccatggat ttttcgtcac tgcggagggg gaaaaatatt
     1501 cagggaaaca gaaagcagag tggaaaaatg ggaaaatatt ctttgatgga aaaacaagcg
     1561 atgaactgtt ttttcaaccc atagacgaat cgaatacttt tgatttattg gatgtagtga
     1621 ttggcattca ttttcattgg gaaagaaaag aaaatcaacg atttaagggt agtttaagaa
     1681 ttattattga aaaggatcaa ttaacagcaa ttaacgatat taaagccgaa gattatcttg
     1741 ttagcgtgat ttcaagcgaa atgagtgcaa cttcttcgct tgaactattg aaagctcatg
     1801 ctgtaatttc cagaagctgg ttgttttatc ctttgcaaaa tgataaaaag aatttttttc
     1861 cacaaaattc tgtggaaaat gacacagaaa atcggcatat caaatggtac gaaagagggg
     1921 aacacgagca tttcgacgtt tgtgccgacg atcattgtca gcgttatcaa ggaattacac
     1981 gagccacttc tgaaactgtt cagcttgcaa ttgaagctac ccgcggcgaa gttttaatgt
     2041 ttgaccacca aatt
//
LOCUS       DAOTKS010000305         2044 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_44198_length_2044_cov_4.283560, whole genome shotgun sequence.
ACCESSION   DAOTKS010000305 DAOTKS010000000
VERSION     DAOTKS010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44198_length_2044_cov_4.283560"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1587
                     /locus_tag="P5236_05970"
     CDS             <1..1587
                     /locus_tag="P5236_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRU63945.1"
                     /translation="NHRSETSDFFTTNELNDTINNGNSDYYSEGNGKNIGGRLDASRQ
                     LGKKGRVLSLQLRGQLNNADNNGNNLSNTFYHGTKPDDIIDQRFTNTNDSKSWQGYIS
                     YVEPIGKNNFIQLAYTYKQNLSESNKDTRTKDANGNYTVMDTTYSKRLENNFTNQSVE
                     LNFKAERGKYDYMVGFSMQPFNSNSKTYKGDSIYPVINQGINYAPTAQFNYRWSRQNN
                     LRIRYSGMTSQPSVTQLSPVMDVSDPLNVRYGNPDLKPSFSHRLNIRYMNFIPEKKSS
                     ITFFGNAAYTLNDIVSSTITDITTGRKETTYKNVNGNWHANGRLTLNLPIRKTNFSIS
                     SMTFGSYNHSKGFSNNEINLSQRTGLGETLGVDFRSSIIDFGIRGNVNYNNVQNSLKG
                     QQNQEYFNYNASANTTLYLPHDFSIDSDISYNTNSGYAAGFEQNAWLWNASIQKQLFK
                     QKNGTLRFKIYDILQQKSNINRSATSNYIRDITTNTLTSYFMVHFVYRFNIFKNGANQ
                     NDMMPRRGFGGPGGPDGNNI"
BASE COUNT          696 a          338 c          340 g          670 t
ORIGIN      
        1 aaccatcgta gcgaaacaag tgattttttc actactaatg aattgaatga tactatcaat
       61 aatggtaatt cggattatta ttcagaagga aatggcaaaa acatcggtgg taggttggat
      121 gccagccgtc aattggggaa aaagggacgt gttctcagcc tccaattaag aggacaatta
      181 aataatgctg ataataatgg caataatctt tccaatactt tttatcatgg aacaaaacct
      241 gacgatatta ttgatcagag atttaccaac actaatgata gcaaaagctg gcaaggatat
      301 atttcttacg tagaacctat tggaaaaaat aattttatcc agcttgccta tacttataag
      361 caaaatcttt ccgaatcgaa caaagacacg cgtaccaaag atgctaatgg aaattatacg
      421 gtaatggata caacttacag taagcgatta gaaaataatt tcacgaatca atcagtggaa
      481 ttaaatttca aagccgaaag aggaaaatat gattatatgg ttggtttcag catgcaacca
      541 tttaattcta acagcaaaac ttacaaaggg gattcaattt atccggttat caatcaagga
      601 attaattatg ctcctaccgc tcagttcaac tatcgctgga gccgacaaaa taatttaaga
      661 atcaggtatt cgggtatgac aagtcagcct tctgttaccc aactttcgcc tgttatggac
      721 gtttccgatc cattgaatgt aaggtatggt aatcctgatt taaagccttc tttcagtcat
      781 cgacttaata ttcgctatat gaattttatt cctgaaaaga aaagttccat tacttttttt
      841 ggtaatgcag cctacacctt aaatgatata gtttcttcaa caattactga catcacaact
      901 ggtcgaaaag aaactactta taaaaacgtg aacggcaact ggcatgctaa tggcagattg
      961 acgctgaatt tgccaataag aaaaactaat ttctctattt cttccatgac ttttggcagt
     1021 tacaatcatt caaaaggttt ttctaataat gaaatcaatt taagccaacg aacaggattg
     1081 ggagaaactt tgggtgtaga ttttcgttcc agtataatag attttggcat tcgtgggaac
     1141 gtaaattaca acaatgttca aaatagtttg aaagggcagc aaaatcaaga atattttaat
     1201 tataatgcct ctgccaacac caccctttat ttacctcatg atttttctat tgatagtgat
     1261 atttcctaca ataccaattc aggttatgcg gcaggttttg aacaaaatgc gtggctttgg
     1321 aatgcttcta ttcaaaaaca acttttcaag caaaaaaatg gtacccttcg ttttaaaata
     1381 tatgatattt tacaacaaaa gagtaatatc aatcgaagtg ctacgtccaa ttatattaga
     1441 gatataacta ccaatacact taccagctat tttatggttc atttcgtata ccgcttcaat
     1501 attttcaaaa acggtgcaaa tcaaaacgat atgatgccac gtagaggttt tggaggacca
     1561 ggtggacctg atgggaacaa tatttaaaaa aatatatcta taaatctcgc ttatttaact
     1621 aataatggtt agtaaaaata aaacaattaa aaaaagatcc ttagataatg tttttataca
     1681 tgtgctggta tggattattg tttttggttt ccctttgttc ttttttgacc gaagaaatga
     1741 attgagttgg tcatttttct ggaatacagc ttcaactatg ttgtatttca tgattatatt
     1801 ttatgtaaat tacctgtttt taattaataa acttcttttt cagcggaaaa cgaaagaatt
     1861 tatttttatt aatctgattt taatcattgt attagctgct ttgatgcatt actcacatga
     1921 gttaattttt agtttgaact ttggtaaaca tttacccatg agaggaagaa gacattcccc
     1981 ttctcccttg aaatttatca taagcaacat gttttctctg acgcttgtgg caggtcttag
     2041 cgtc
//
LOCUS       DAOTKS010000306         2035 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_44440_length_2035_cov_4.855556, whole genome shotgun sequence.
ACCESSION   DAOTKS010000306 DAOTKS010000000
VERSION     DAOTKS010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44440_length_2035_cov_4.855556"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..147)
                     /locus_tag="P5236_05975"
     CDS             complement(<1..147)
                     /locus_tag="P5236_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HRU63946.1"
                     /translation="MSQSKFKIILNGLITENPIFVLILGMCPTLATSNSALNGISMGL
                     ATSFV"
     gene            complement(164..832)
                     /locus_tag="P5236_05980"
     CDS             complement(164..832)
                     /locus_tag="P5236_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HRU63947.1"
                     /translation="MKKLESSLKNMVGVLLAITVISALTLSSVYSVTKDPIEKSKTVK
                     QQNAIKEVIPGFDHLATPEKISLNSGDTLTIYKAYDKDNVFIGAAVESFSKKGFGGEI
                     KVMVGFDKEGNIINYSVLEQKETPGLGTKMVDWFKTPIGNQDIRGKNPAKNNLTVKKD
                     GGEVDAITAATISSRAFLDAIHNAYAAYSRNFENKEENKIDSESGASKIEHQDSTKKD
                     SMNN"
     gene            complement(829..1794)
                     /locus_tag="P5236_05985"
     CDS             complement(829..1794)
                     /locus_tag="P5236_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HRU63948.1"
                     /translation="MNNLVVSPAPHIHSGNSVPKEMKGVLIALIPAYLAALYYFGLGA
                     FIVSITAVISCVAFEYLIQRFLLKGELTINNLSAVVTGLLLSFNLPSNLPIWIIIIGS
                     LAAIGIGKMSFGGLGNNPFNPALIGRVFLLISFPVQMTTWPKPIPFNIAYLDAETSAT
                     ALTLLKEHTGTLPSTLDMFLGNIGGSLGEVSALALLIGFVYLLVKKIISWHIPISILA
                     TVAIFAGILHFIHPEMCASPLTHLLTGGLLLGAIYMATDYVTSPMSKLGMIIYGIGIG
                     LITVIIRVWGSYPEGMSFAILIMNGFTPLINAYVKPKRFAEVVKQ"
     gene            complement(1811..>2035)
                     /locus_tag="P5236_05990"
     CDS             complement(1811..>2035)
                     /locus_tag="P5236_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transporter RnfC"
                     /protein_id="HRU63949.1"
                     /translation="RCAKCVFSCPMGLEPYLLMNFGEKMLWEEMEKNQVLDCIECGSC
                     SFICPSHRPLLDYIKNGKVAINNIKINSNN"
BASE COUNT          689 a          386 c          303 g          657 t
ORIGIN      
        1 tacaaatgaa gtagcaagtc ccatgctgat tccatttaaa gctgaattag aggtagctaa
       61 cgtggggcac attcccaaaa tcaacacaaa tatgggattt tccgtgatta aaccgttcag
      121 gatgatttta aatttagact gactcatttt ttatcatttt ttattaattg ttcattgaat
      181 cttttttggt agaatcctga tgttctattt ttgaagctcc actttcagaa tcaattttat
      241 tttcttcttt gttttcaaaa tttcttgaat aagcagcata tgcattatga atagcatcca
      301 aaaatgcacg ggagctaata gttgcagccg taatggcatc aacttcaccg ccatcttttt
      361 taacggtaag attatttttt gctggatttt ttcctctaat gtcttgattg ccaattggag
      421 ttttaaacca gtccaccatt tttgttccca agccaggagt ttctttttgt tctaacactg
      481 aataatttat gatatttcct tctttatcaa aacctaccat aacttttatt tctcctccaa
      541 atcctttttt tgaaaaagat tcaacagcgg ctccaataaa gacattatcc ttatcatagg
      601 ctttataaat agttaatgtg tcgccgctat ttaagctaat tttttcagga gttgctaaat
      661 ggtcaaaacc tggaattact tctttaatag cattttgttg ctttactgtt tttgattttt
      721 caatcggatc tttagttact gaataaacgg aacttaaagt gagagctgaa attactgtga
      781 ttgccagcaa cacaccgacc atatttttga gacttgattc taattttttc attgtttaac
      841 tacctccgca aaacgttttg gtttaacata agcattgata agtggtgtaa aaccattcat
      901 aatcaaaata gcaaatgaca ttccttcagg ataggaaccc caaacccgaa taataacagt
      961 tatcagccct attccaatgc cataaattat cattcctaat tttgacatgg gagaagtaac
     1021 ataatccgtt gccatataaa tagctcctaa caataaacct cctgtcaaaa gatgagtaag
     1081 tggtgaagca cacatttcag gatgaataaa atgaagaatt cctgcaaaaa tagccacagt
     1141 agccaatatg ctgattggaa tatgccagct aataattttt ttcaccagca aataaacaaa
     1201 acctatcaac aaagccagtg ctgaaacttc gcctaacgaa ccacctatat tcccgagaaa
     1261 catatccagc gttgaaggaa gtgttccagt atgttctttt aacaatgtaa gtgctgtagc
     1321 tgaagtttct gcatccaaat aagctatatt aaatggaata ggtttaggcc aggtagtcat
     1381 ttgtactgga aaagaaatta acaaaaatac acgtcctatc aatgctggat taaagggatt
     1441 gttccccaaa cccccaaagc tcatttttcc tattccgata gcagctaatg aacctattat
     1501 aattatccag ataggaagat tggaaggtaa attaaaagaa agcaacaatc cagtcactac
     1561 tgctgaaaga ttattaatag tcaattcacc tttcaacaaa aatcgttgaa tgagatattc
     1621 aaaagccaca catgaaatca ctgcggtaat acttacaata aaagcaccca gaccaaaata
     1681 atacaaagca gctaaataag ctggaattaa tgcaattaat acacctttca tttctttagg
     1741 aacggaattc ccgctatgaa tatgaggtgc tggcgaaaca actaaattat tcatatttga
     1801 attctatatt ttaattattg ctatttattt ttatattgtt aattgcaact ttaccatttt
     1861 tgatataatc caataaagga cgatgagaag gacaaataaa actacaggag ccacattcaa
     1921 tacaatcaag aacctggttt ttttccattt cttcccacaa cattttttcc ccaaaattca
     1981 ttaataaata aggctccaac cccatcggac aggaaaaaac acattttgca cagcg
//
LOCUS       DAOTKS010000307         2032 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_44542_length_2032_cov_3.206879, whole genome shotgun sequence.
ACCESSION   DAOTKS010000307 DAOTKS010000000
VERSION     DAOTKS010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44542_length_2032_cov_3.206879"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1371
                     /locus_tag="P5236_05995"
     CDS             <1..1371
                     /locus_tag="P5236_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRU63950.1"
                     /translation="LKYGTGVNDSTSWEKRKNTDVKNFRLEGALYYKENENSSGYLKS
                     YFYSSERGLPGATILYLTENSNRQRSWDRTFFTQAHYEKKISSLWSFGFNAKYHYGFL
                     KYLDPDVNNSLGKIENIYRQQEYYSSFSFLFQAFENLSFSSSTDIACNTLSANLTDFA
                     YPTRWTLLSCLNGKYVNHFLLTTAGLLLTNVNDKVRYGKAPENYQQLSPYASISVKPF
                     NKYDVRFRCFYKNIFRMPTFNDLYYVYVGNRDLKPEKSHQFNAGVTYCTSLENWLPLL
                     TITWDVYHNEVKNKIIAFPQKDIFNWTMKNYGKVLIDGLDLSVEGSFSLSEKIGIIAG
                     TTYTYQKAINVTDPSEENYRHQIPYTPRVSGSGRAALQTPWLNIAYSFLWSGKRYALF
                     QNYTENRLSGYADHSISLQKEIKIKQQALSLNVEILNLWNTNYEIIRWYPMPGRSFRI
                     TAATRF"
BASE COUNT          682 a          336 c          314 g          700 t
ORIGIN      
        1 ttaaaatatg gcacaggagt caatgacagc acttcatggg aaaaaagaaa aaataccgat
       61 gtaaaaaatt tcagattaga aggagctctt tattataagg aaaatgaaaa tagttcaggc
      121 tatttgaaaa gttattttta ttcttctgaa cgtggattgc caggagcaac tattttgtat
      181 cttaccgaaa actcaaaccg ccagcgaagt tgggacagaa cattttttac acaagctcat
      241 tatgaaaaaa aaatatcctc gctgtggtct ttcggtttca atgcaaaata tcactatggt
      301 tttttaaaat atcttgatcc tgatgtcaac aacagtttgg gaaaaataga aaatatttat
      361 cgacagcaag aatattacag ttctttcagt ttcctttttc aagcttttga aaacttgtcg
      421 ttttcttctt caaccgatat tgcttgcaat actctctctg caaatttaac tgattttgct
      481 tatcccacac gttggacttt actttcctgc ctgaatggaa aatatgtaaa tcatttttta
      541 ctgactaccg ctggtttgct tttaaccaac gtaaatgata aagttcgata tggtaaagca
      601 cccgaaaatt atcagcaact ttctccttat gcaagtattt ctgtaaaacc ttttaacaaa
      661 tatgatgtac gttttcggtg tttttataaa aacatttttc ggatgcctac tttcaacgat
      721 ttatattatg tttatgttgg caatcgagat ttaaagcctg aaaaaagtca tcaattcaat
      781 gcaggtgtta cttattgtac ttctcttgaa aactggctgc cattgttgac cataacatgg
      841 gatgtttatc acaatgaagt gaaaaacaag attattgctt ttccacaaaa agatattttt
      901 aattggacaa tgaaaaatta tggaaaagtt ttgattgatg ggttggattt atccgtagaa
      961 ggttcgtttt cgttatccga aaaaattgga ataattgcag gaactactta tacttaccaa
     1021 aaagctatta atgtaactga tccatcagaa gaaaattatc gtcatcaaat cccttataca
     1081 ccaagagttt ctggttcagg aagagctgcc ttgcaaacgc cctggcttaa tattgcctat
     1141 tcgttcctct ggtcgggtaa aagatatgca ctttttcaaa attatacaga aaatcgtttg
     1201 tcgggctatg ccgatcatag tatttccttg caaaaagaaa taaaaatcaa acagcaagct
     1261 ttatcactga atgtggaaat attgaattta tggaatacga actatgaaat aattcggtgg
     1321 taccccatgc ctggtcgttc attccgtatc accgcagcca ctcgattttg agaagaaagt
     1381 aaatgttatt ttcagaatga aacgcacaga aaattttttt attgaaactt tgagaaattt
     1441 tctcttttta gttacattga gaaatgccta ttgtcctgtg tggctctaat aaaatgaggt
     1501 tttgcttcaa cccacactta ctattttacc tcaaaaaata cttttttatt gtttcagtaa
     1561 tttcatcttg tttaagattt ttctctggct gcactttgaa aatgaattgt ttataacaca
     1621 gagaaatact gagatttttt cttcattcgc tcaaaactcg aaacctgtca attttcggtg
     1681 tattggacaa ggtctatcca aaacaacata gttaatcgat aaatatcaat ttaatataaa
     1741 atttatcaga tactaaaaac aaaatatttg attgattttt taatatttta cagccttttt
     1801 attaaacttt ctaaatactt attttttcaa tgacaattct tggcttagaa acaaaagtac
     1861 ctgaaaataa atcaattgtt gtgttaaacc cgtataatta taaataattc tacaattttc
     1921 tggtaactgc cactgttgga atactttcct gtttaaaacg attgatagca gtgacaacaa
     1981 aagtatagtg tcctttcaaa ggaacattca aagctctcaa atcaagacaa tt
//
LOCUS       DAOTKS010000308         2022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_44837_length_2022_cov_3.850534, whole genome shotgun sequence.
ACCESSION   DAOTKS010000308 DAOTKS010000000
VERSION     DAOTKS010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44837_length_2022_cov_3.850534"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="P5236_06000"
     CDS             complement(<1..354)
                     /locus_tag="P5236_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63951.1"
                     /translation="MKEKVKTYKNHFFALFILMIIGFNACTPSKKGITILNSGTDSYT
                     SLQISNPFGRAVTFIGNENVGAIGYELDGKTFWVKGYPEITVLVDSTLNYCWKQEERQ
                     VIMNVKEKEKETRFKF"
     gene            complement(908..>2022)
                     /locus_tag="P5236_06005"
     CDS             complement(908..>2022)
                     /locus_tag="P5236_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HRU63952.1"
                     /translation="HIYVQSHPEEVRKEADAAVKFLGFKGDYFVDADHVNLNTVEPFI
                     ASSDFFTLDVASYIGKSTSNENINAFINSCKNFNKELSIPGIEEKFEISNEWLENIAK
                     KFLLASEQAAVLYQFIKEKKKNNPFIVEVSMDEVPTPQTPLELFFILKMLADKKIPLQ
                     TIAPKFTGRFNKGIDYQGDIKQFAKEFEEDILVIDYAIKMFNLPQDLKLSIHSGSDKF
                     SIYPFIAEITKKHNKGFHLKTAGTTWLEEIIGLAAAGNEGLILAKKIYAIAFQRKNEL
                     CEPYSDVIDIDENKLPRPEEVETWTSEKFVNTLRHVPDHPDYNPNFRQLMHVAYKIAA
                     EMKNEYFNLLEKYSKIIETCVEENIFDRHLKRLFDL"
BASE COUNT          670 a          314 c          281 g          757 t
ORIGIN      
        1 aaatttgaat cgtgtttctt tttctttttc ctttacgttc attattactt gtctttcctc
       61 ctgtttccag caataattca gggtgctgtc tactaaaact gtaatttcag gataaccttt
      121 cacccagaag gttttaccat ctagctcata acctatagct cctacattct cattgccaat
      181 aaaggtaacg gctcttccga aaggattact tatttgcaga gaggtatatg aatcagtgcc
      241 agaattcaaa atggtaattc ccttttttga tggagtgcag gcattaaatc caattatcat
      301 aaggataaat aatgcaaaaa aatgattctt ataggttttt actttttctt tcattttgat
      361 aggtttaata tctttttgtt attttaatga ttatctgcaa ttcatgctgg gattaaattt
      421 aatttggaat aatatgatgt tatttttaca taggaaccct ggaataccca gaccactccg
      481 aaaagtttct cgaaattaat ttattcatag ttaattgtta taaaaagtat aaaaaacacc
      541 tgaaaaagtt ttttgaaaaa tttgtatgta tcattatatt tcaatatctt acctttaatc
      601 ttaaagtgag cttactctcg tttattggga ataaacttca tgaatactga aatatattat
      661 ccaaactatt taaggagtat ttaatgcttt ataaaaagct aattttttag taaaaataac
      721 ttttatatat ggcaaataat tgtaaaatat caaaacaata aaaaatttaa tattccttca
      781 aaaaatctca ttttttcttc cttttgaaaa aatcaggttt ttaagagaga actcatcttt
      841 ttcaattaca ggatcattgt tataaggata ttctttataa gaggtttttg aaataattac
      901 agaaaattta taaatcaaac aaacgtttta gatgccgatc aaaaatattt tcttctacac
      961 aagtttcaat tatttttgaa tatttttcga gtaaattaaa atattcgttt ttcatttctg
     1021 ctgctatttt ataagctaca tgcattaatt ggcgaaaatt aggattataa tcaggatgat
     1081 caggaacatg acgtaaggta ttcacgaact tttcactcgt ccaagtttca acttcttcgg
     1141 gacgtggtaa tttattttca tcgatgtcaa tcacatctga gtaaggctcg cacaattcgt
     1201 tttttctttg aaaagcaata gcatagattt tttttgcgag gatgagacct tcattcccag
     1261 cagccgctaa accaattatt tcttccaacc aggtagtacc ggctgttttc aaatgaaatc
     1321 ctttattatg ttttttagtt atctcagcaa taaaaggata aatagaaaat ttgtcgctcc
     1381 ctgaatgtat gctcaatttt aaatcttgtg gcagattaaa catttttata gcataatcta
     1441 taactaatat atcttcttca aattcttttg cgaattgttt tatatcacct tgataatcaa
     1501 tccctttatt aaatctacca gtaaactttg gagcaatggt ttgaagaggt atcttcttgt
     1561 cagccagcat tttaagaata aaaaagagtt ccagtggagt ttgtggagtg ggcacttcat
     1621 ccatagaaac ttcgacaata aatggattat tctttttctt ttcttttata aattgataaa
     1681 gcacggcggc ttgctctgat gctaaaagaa attttttagc aatattttcg agccattcat
     1741 tacttatttc aaacttctct tcaataccag gaattgataa ttctttatta aaatttttac
     1801 aggaattaat aaaggcatta atattttcat ttgaagtaga tttacctata taagaggcta
     1861 catccaatgt aaagaaatca gatgaagcta tgaaaggttc cactgtattt aaattaacat
     1921 gatctgcatc aacaaaataa tctcctttaa atcctaaaaa tttaacagca gcatcagctt
     1981 ctttgcgaac ctcttcagga tgtgattgaa cgtaaatgtg tt
//
LOCUS       DAOTKS010000309         2019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_44925_length_2019_cov_3.204175, whole genome shotgun sequence.
ACCESSION   DAOTKS010000309 DAOTKS010000000
VERSION     DAOTKS010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44925_length_2019_cov_3.204175"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1428
                     /gene="lysS"
                     /locus_tag="P5236_06010"
     CDS             <1..1428
                     /gene="lysS"
                     /locus_tag="P5236_06010"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRU63953.1"
                     /translation="VFKKLLDIGDFIGIKGFVFRTQMGEITIHAQELTVLAKSLRPLP
                     IVKYKDGVAYDAFNDPEQRYRQRYIDLVVNEGVKDIFLKRTQIFQSMRDYFNAQGYVE
                     VETPILQPIPGGAAARPFVTYHNALDMQLYLRIADELYLKRLIVGGFEGVYEFSKNFR
                     NEGMDRTHNPEFTAMEIYVAYKDYFWMMEFTERLLEKICLDVNNSTEIHLENKVIDFK
                     APYKRIKMLDVIKENTGIEIDGMDENQLREVCRQLDIEVDETMGKGKLIDKIFSEKCE
                     ANLVQPTFIIDYPIEMSPLCKRHRNNPELTERFELIVNGQELANAYSELNDPIDQLER
                     FKEQLRLSEKGDEEAMFIDMDFIRALEYGMPPTSGLGIGMDRLVMLLTGQQAIQEVLL
                     FPQMKPEKVIKSDDKEKFLHLGIKEPWLDVMEKIGIKTIEQLKVFNPNKLHQDLCSWN
                     KKNKLELVNPTIEEVNNWISKAKDN"
     gene            1470..>2019
                     /locus_tag="P5236_06015"
     CDS             1470..>2019
                     /locus_tag="P5236_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRU63954.1"
                     /translation="MFELIFELSSHVDTTIFYGINNVNIQLIKNLYPNLKIVARGHII
                     KIMGSEEEADKLFLNLQKLEQYSIENNKLTEKNIIDLIKDNVPTDIVKYENLILHGSG
                     GKSIIARTKNQQKLVKEFETNDLLFAIGPAGSGKTYTAIALAVRSLKNKEVKRIILSR
                     PAVEAGEKLGFLPGDMKEKIDPY"
BASE COUNT          738 a          297 c          390 g          594 t
ORIGIN      
        1 gtttttaaaa aactgcttga cattggtgat tttattggta taaaaggctt tgtttttcgg
       61 acacaaatgg gcgaaataac tattcatgct caagaattga ctgtactcgc aaaatcgctg
      121 cgaccactgc ctattgtgaa atacaaagat ggtgtggcgt atgatgcttt caatgaccct
      181 gaacaacgtt atcgtcagcg atatatagat ttggttgtaa acgaaggtgt aaaagatatt
      241 tttctgaaac gcacgcaaat ttttcaatcc atgcgggatt attttaatgc acaaggttat
      301 gttgaagtag aaacacctat tttacaacct attccaggtg gagctgctgc tcgtccattt
      361 gtaacctatc acaatgcact cgatatgcaa ctttatctca gaatagcgga tgaattatat
      421 ctcaagcgat taatagttgg cggttttgaa ggtgtgtatg aattttcaaa aaatttcaga
      481 aacgaaggaa tggatcgaac acataatccg gaattcacag caatggaaat ttacgtagcc
      541 tataaagatt atttctggat gatggaattt actgaacgat tacttgagaa aatttgttta
      601 gatgtaaata acagtactga aattcacttg gaaaataaag ttattgattt taaggctcct
      661 tataaacgca tcaaaatgct ggatgtaatt aaggaaaata caggaatcga gattgatgga
      721 atggatgaaa atcaacttcg ggaagtttgc cgtcagttag atattgaagt ggacgaaaca
      781 atgggcaaag gaaaattaat tgataaaatt tttagtgaaa aatgtgaagc taatctggtg
      841 caacccactt ttattataga ttatccaata gaaatgagcc cattatgtaa acgtcatcgc
      901 aataatcctg aattaactga acgttttgaa ctcattgtca atggtcaaga actagccaat
      961 gcttattctg agttgaatga cccgattgac caattggaaa gattcaagga acaactcagg
     1021 ttgagtgaaa aaggcgacga agaagcgatg tttattgaca tggattttat acgagcctta
     1081 gaatacggga tgccaccaac ttcgggcttg gggattggga tggacaggtt agtaatgctt
     1141 ttgactggtc aacaagctat tcaagaagtt ttactcttcc ctcaaatgaa acctgaaaaa
     1201 gtaataaaat cggatgataa agagaaattc ttgcatttag gaatcaaaga accgtggctg
     1261 gacgtcatgg aaaaaattgg tataaaaaca atagaacaat taaaagtttt taatcccaat
     1321 aaattacatc aggatctgtg cagttggaac aagaaaaata aacttgaact tgttaatccg
     1381 acgattgaag aagtaaataa ttggatatca aaagcaaaag acaattaaaa aattaatttc
     1441 tcatttttaa aaaatttcgc taataaaata tgtttgaact tattttcgaa ctttcttcac
     1501 atgttgacac taccattttt tatggaataa acaacgttaa tattcaatta atcaagaatt
     1561 tatatcctaa tttaaaaatt gtagcacgag gacatatcat aaagatcatg ggaagtgagg
     1621 aagaagcaga taagttgttt ttaaaccttc aaaaactgga acaatacagt attgaaaaca
     1681 ataaattaac agagaaaaat attattgacc tgataaaaga taatgtccct actgatatag
     1741 taaaatatga aaatcttatt cttcacggga gtggtggcaa atctattata gctcgaacta
     1801 aaaatcagca aaaactggta aaagaatttg aaaccaatga cttgcttttt gcaataggac
     1861 cggcgggttc aggaaaaact tacacggcta ttgcgttagc ggttcgttct ttaaaaaata
     1921 aagaagttaa gcgaattatt ttaagtcgtc cagccgtaga agccggtgaa aaattaggtt
     1981 ttttgccggg tgacatgaaa gaaaaaattg atccttacc
//
LOCUS       DAOTKS010000310         2017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_44990_length_2017_cov_4.306830, whole genome shotgun sequence.
ACCESSION   DAOTKS010000310 DAOTKS010000000
VERSION     DAOTKS010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44990_length_2017_cov_4.306830"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /gene="thrC"
                     /locus_tag="P5236_06020"
     CDS             <1..908
                     /gene="thrC"
                     /locus_tag="P5236_06020"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007483875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRU63955.1"
                     /translation="TGSAVANGFLGIEGIHVYVLYPKGLVSSIQECQFTTLGENITAL
                     EIEGTFDDCQRLVKSAFVDPELNACFKLTSANSINIARLLPQSFYYFYAFAQLRAIHP
                     KYTAEELEMVICVPSGNFGNLTAGLIAKRMGLPIKRFIAANNRNDVFLEYLQTGEYHP
                     RTSVATIANAMDVGNPSNFDRILDLYGYSHEAITKDISGISYTDEQIAATIKNCFSKN
                     GYLLDPHGACGYQALKDKLLPEENGIFLETAHPAKFLDTVEKIIGEKIEIPAKLQEFM
                     KGEKKSIPLSKDFEEFKKFLLANAH"
     gene            complement(1117..>2017)
                     /locus_tag="P5236_06025"
     CDS             complement(1117..>2017)
                     /locus_tag="P5236_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63956.1"
                     /translation="NPPSLSSLSPLMATLEENLPVNIVIANILSFVLTIFNGMLAEHL
                     NNRFTIIHARTFLPFFIFLMLMSTWYGIHSMWILQIVLTFFIGSFFFLFDMFHQNQPV
                     EQAYIGSLLIGLCSLFLPSLIFIIPLFWVGFFQFQNFTLKTFLASLLGWLTPFVLYFG
                     FYFVFQPQVDLYPLFVQYFHFESNFSFKPIMSIESIYGGLFALLLLVIIGSIYNDFQK
                     NAIPTRKRLNFLVFSLLLFIVLSLLNFYQRIFYLPFFAYISSLLFSHAFTLKENKFFS
                     VLFIVFCLLNIAFVFSKLLIDFG"
BASE COUNT          812 a          333 c          321 g          551 t
ORIGIN      
        1 acactggaag tgcagtggca aacggttttt tgggtatcga gggaattcat gtgtatgtcc
       61 tttatccaaa aggtttggta agctctatcc aagaatgtca gtttaccaca ttaggggaaa
      121 acattaccgc actcgaaatt gaaggaacat ttgatgattg tcagcggctg gtaaaatcag
      181 cctttgtcga cccagaacta aatgcttgct ttaagttgac ttcagccaat tccattaata
      241 ttgctcgact attgccacaa tctttttatt atttctatgc ctttgcccaa ttaagagcca
      301 ttcatccaaa atatactgct gaagagctgg aaatggttat atgtgttccc agtggaaatt
      361 ttggcaatct tacggcagga cttattgcca aacgaatggg acttcccatt aagcgattta
      421 ttgcagccaa taatcgcaat gatgtttttt tagagtatct tcaaacaggc gaatatcatc
      481 cacgtacttc tgtggctacc atcgccaatg cgatggatgt gggcaatcca agtaattttg
      541 atcggatact cgatttgtat ggctattctc acgaagctat taccaaagat ataagcggaa
      601 tcagttatac tgatgagcaa atcgctgcaa cgatcaaaaa ctgtttctct aaaaatggtt
      661 atttactcga tcctcatgga gcttgtggct atcaagcctt gaaagacaaa ttattacctg
      721 aagaaaatgg tatatttttg gaaacagctc atcctgcaaa gtttcttgat acggtagaaa
      781 aaattattgg tgaaaaaatt gaaatccctg caaaacttca ggaattcatg aaaggagaaa
      841 agaaaagtat tccgttaagt aaagattttg aagaatttaa aaagttctta ttggcaaatg
      901 ctcattgatt ttttcagaaa atgacaaata aatatttttt aaactacaac aaaaataatg
      961 tatcaccacc ctcctcaatt tatcaaagaa tttaccagaa aacaaatcca ttatccaaac
     1021 ctgtaaaatc ataccatacc tgtaagcact aaaagaaaat aactatcctg accagcaata
     1081 aagtcaaaaa taacctgctt caacaaatat ccttttctat ccgaaatcaa tcaacaattt
     1141 cgaaaaaaca aatgctatat ttaataaaca aaaaacaata aatagcacac taaaaaattt
     1201 gttttccttc aaggtaaaag catgtgaaaa taacaaagaa gaaatataag caaaaaaagg
     1261 aaggtaaaat attctttgat aaaaattcaa caaagaaaga acgataaata ataataaaga
     1321 gaacacaaga aaattcaatc tttttcgagt agggatagca tttttttgaa aatcattata
     1381 aatgctgcca attattacca aaagcaacaa agcaaataaa cctccataaa tactttctat
     1441 actcataata ggtttaaaag aaaaattact ttcaaaatga aaatattgaa caaacaaggg
     1501 atacaaatcg acttgaggtt gaaaaacaaa ataaaaacca aaataaagta caaaaggggt
     1561 taaccagccc aataaagatg ccagaaaagt ttttaaggta aaattttgaa actgaaaaaa
     1621 acctacccag aatagaggaa taataaatat taaagaaggc aaaaataaac tacaaagtcc
     1681 tattaataaa ctaccaatat aggcttgctc aactggttgg ttttgatgaa acatatcgaa
     1741 caaaaaaaag aaagaaccaa tgaaaaaagt tagtactatt tgcaggatcc acatcgaatg
     1801 aatgccatac catgttgaca tcaacatcaa gaaaataaaa aatggtaaga aggttcttgc
     1861 atgaataatg gtgaaacgat tgtttaaatg ttcagccaac atcccattaa aaatagtcaa
     1921 tacaaaactt aaaatattag cgattacaat attaactgga agattttcct ctaaggttgc
     1981 catcaaaggg gaaagagaag acaaagaagg aggattt
//
LOCUS       DAOTKS010000311         2009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_45234_length_2009_cov_3.335722, whole genome shotgun sequence.
ACCESSION   DAOTKS010000311 DAOTKS010000000
VERSION     DAOTKS010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45234_length_2009_cov_3.335722"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="P5236_06030"
     CDS             <1..348
                     /locus_tag="P5236_06030"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294924.1"
                     /note="catalyzes the release of the N-terminal amino acid
                     from a tripeptide; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HRU63957.1"
                     /translation="LVNKINAEFGENTAIIELKDQYYNMREKIEPVMYIIDIASQAMK
                     EVGVTPKISPIRGGTDGARLSYMELPCPNIFAGGHNFHGRFEYIPIPSMEKAMMVIVK
                     IAELVDNQENISQ"
     gene            410..1480
                     /gene="gcvT"
                     /locus_tag="P5236_06035"
     CDS             410..1480
                     /gene="gcvT"
                     /locus_tag="P5236_06035"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRU63958.1"
                     /translation="MKTTPFTHFHLELGAKMQEFAGYNMPIEYSSISDEHLAVRQKVG
                     VFDVSHMGEILVKGPKALAFLQKMTTNDVASLPLGKAQYSCFTNEAGGIIDDLLVYHI
                     EPEKYLLVINAANVEKDWEWCVLNNSENAEIENISDQVAQLAVQGPLAVQVIQKLTSI
                     NLSEIPYYHFTIGKVVEVEPVLLSNTGYTGAGGFELYFSPEYADKIWNALFEAGKEFG
                     IQLIGLGARDTLRLEKGFCLHGNDIDETTTPLEAGLDWITKFLPEKNFIGQAALEKQK
                     QKGIQRKLIRFELLEKGIPRHGYDILNQHNEIIGKVTSGSFIPGDKTGIGMGYIKTDY
                     AKKNTEIYIQIRNKSLPAKIVK"
     gene            complement(1543..>2009)
                     /locus_tag="P5236_06040"
     CDS             complement(1543..>2009)
                     /locus_tag="P5236_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HRU63959.1"
                     /translation="AKLTYRTVKLFAHEPVMAMLSYSNFGSDKQGSPASVHEVVATLH
                     REYPEMIVDGEMQVNFALDKELRDRKYPFSKLAGKDVNTLIFPNLSSANSAYKLLLEI
                     GLAESIGPIQMGLNKPIHILDVESSVRDIVNMTTIAVIDAMLEEQKNSGKVS"
BASE COUNT          679 a          355 c          357 g          618 t
ORIGIN      
        1 cttgtcaata aaataaatgc tgaatttggc gaaaatacag caattattga attaaaagat
       61 caatattaca acatgcgtga aaaaatagag cccgtaatgt atatcattga tatagcttca
      121 caagccatga aagaagttgg tgtaaccccg aaaatttctc cgattcgtgg tggcaccgac
      181 ggagcacgac tttcttatat ggaactcccc tgccctaaca tttttgcagg aggacataat
      241 tttcatggac gttttgaata cattcccatt ccttctatgg aaaaagccat gatggttatt
      301 gtaaaaattg cagaactggt tgacaatcaa gaaaatattt ctcaataatt ttatcaaaaa
      361 tcatttttat ttatttttca gaaaaaattc aaaatcaaaa aattcatcaa tgaaaaccac
      421 tccatttact cactttcatc ttgagcttgg agctaaaatg caagaatttg ccggttacaa
      481 tatgcctatt gaatatagca gtatcagcga tgaacatctg gcggttcgtc agaaagtcgg
      541 tgtttttgat gtttcacaca tgggcgaaat tcttgtaaag ggaccgaaag cgttagcctt
      601 tcttcaaaaa atgacaacca acgatgttgc ttctttgcct cttggtaaag ctcaatattc
      661 ctgttttact aacgaagcag gtggaataat agatgatttg ttggtttatc atatagagcc
      721 tgaaaaatat ttattggtca tcaatgctgc taacgttgaa aaagattggg aatggtgtgt
      781 tttaaataac agcgaaaatg ctgaaataga aaatatttca gatcaggtgg ctcagttagc
      841 cgtacaaggt cctttagctg tccaagtcat tcaaaaatta acgtctatta atttatcaga
      901 aattccttat tatcatttta cgataggaaa agttgttgaa gtagaaccag ttctcttatc
      961 taatacgggt tacactggag ctggcggttt tgaactatat ttctcgcctg aatatgctga
     1021 taaaatatgg aatgcacttt ttgaagcagg gaaagaattt ggtatccagc ttatcgggct
     1081 tggtgctcgc gatactttac gcctcgaaaa aggattttgc cttcatggca atgatattga
     1141 tgaaactacc actccattag aggcaggact ggattggatt accaaatttc tcccggagaa
     1201 aaattttatt ggtcaagccg cacttgaaaa acaaaaacaa aaaggtattc aacgcaaatt
     1261 aataagattt gagctgttag aaaaaggtat tccccgtcat ggatacgata tattaaacca
     1321 gcataatgaa ataattggga aagtaacttc gggaagtttt attccaggtg acaaaaccgg
     1381 aattggaatg ggatatataa aaacagacta tgcgaaaaaa aatactgaaa tttatattca
     1441 aatcagaaac aaaagtttac ccgcaaaaat tgtaaaataa aaatcaaaaa gctgcttttc
     1501 tgaagaaaaa aggcagcttt ttacttagtt tttcaatatt atttaactta ccttcccact
     1561 atttttttgt tcttccagca tggcatcaat tacagcaatg gttgtcatat tgacaatatc
     1621 acgcactgag ctttctacat ccagaatatg aataggttta ttcaaaccca tctgaatcgg
     1681 tcctattgat tcggccagac ctatttccag caataattta taagcactgt ttgccgaact
     1741 taaattaggg aaaattaagg tatttacgtc ctttcctgct aatttggaga aaggatattt
     1801 tctatcacgt aattctttat ccagagcaaa attaacctgc atttcaccat caacaatcat
     1861 ttccggatat tctctgtgca aagtggcgac cacttcatga acacttgccg ggctgccttg
     1921 cttatcggaa ccaaaattgg aataagacaa catcgccatt actggttcat gagcaaaaag
     1981 ttttacagtt cgataagtca attttgcaa
//
LOCUS       DAOTKS010000312         1998 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_45557_length_1998_cov_4.436438, whole genome shotgun sequence.
ACCESSION   DAOTKS010000312 DAOTKS010000000
VERSION     DAOTKS010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45557_length_1998_cov_4.436438"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            310..714
                     /locus_tag="P5236_06045"
     CDS             310..714
                     /locus_tag="P5236_06045"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HRU63960.1"
                     /translation="MARPQHQRKIASPPLMMGYQPFGIFPKESVKIILHLDEYEALRL
                     VDYEGLQQTEAAKKMQISRPTLTRIYENARKTIAKAMVEGKTIVIEGGNVEFDQQWFR
                     CKHCFKLVNEKETHIHTNEEEKENLLPLLTNK"
     gene            758..1633
                     /locus_tag="P5236_06050"
     CDS             758..1633
                     /locus_tag="P5236_06050"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HRU63961.1"
                     /translation="MQESGINEQCKFCFVQNFERLIEKFPINESDREKFEKHYEKILR
                     ESDGKLMPEIYRELNKEFLKLSGLSDPFAEEKRKSNEIALELYNDLKQVVEKSENPFQ
                     LALRLAIAGNIMDYGPTAHFDVHSTIEKAFTIQFAIDHSVELEKRIKIAKKILYLGDN
                     AGEIVFDKLFIETMKHPNVTFAVRGAPALNDVTLEDAYQVGIDKVARVISNGNDVAST
                     IVKECSSEFQEIFQEADLIISKGQGNLEGLIYQNDPRIFFLLVAKCDVIAQLLGVEKG
                     SFVVYNTQLPKNKNL"
     gene            1630..>1998
                     /locus_tag="P5236_06055"
     CDS             1630..>1998
                     /locus_tag="P5236_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63962.1"
                     /translation="MTQSLIFTILALFLGSVGSVLLAGLILLLKEKRLQQITRFLTYL
                     AGGTLLGSALVGMIPEALAVLKPEIVLNVLLGGLVFFFVLEKIILWRICADASCERNL
                     LAAAPMVLFGDAFHNAIDGVI"
BASE COUNT          699 a          263 c          383 g          653 t
ORIGIN      
        1 gattgttgct tccgattgca aagttacgtg ataattttca atttcgcatt gataagtaaa
       61 gatcaattaa ttgctaacta aaaaagtgcg aggtgtggcg ttcgagttac gaggtgcgag
      121 tcttggttta taggatttaa ttgataatcg tgaatttaga gtttgtagta gaaacatttt
      181 acgaagccta ttttataatt aaagccaaat tatttttatt acatagaatt tttttcttta
      241 aaaaataatg aacgtaagtt caaaattttt tttgtaattt tgccttgttt taaaaaatcg
      301 aaaaagatta tggctcgtcc tcaacatcaa cgtaaaatag cttcaccacc acttatgatg
      361 ggctatcagc cttttggaat ttttcctaaa gaatcagtaa aaataatttt acatttagat
      421 gaatatgaag ccctccgttt ggtggattat gaaggattgc aacagactga agctgctaaa
      481 aagatgcaaa tttcaagacc aactttaaca cgaatttacg aaaatgcacg taaaaccata
      541 gcaaaagcaa tggtagaagg aaaaacgatt gtaatagaag gaggtaatgt ggaatttgat
      601 cagcaatggt ttcgctgcaa acattgtttt aaattagtga atgaaaagga aacacacatt
      661 cacaccaatg aagaggaaaa agaaaattta ttacctttat taactaataa gtaattaact
      721 gatttacaat taaaaaaact ataatatatt aataaaaatg caagaaagtg gaattaatga
      781 acaatgcaaa ttttgctttg tacaaaattt tgaaagatta atagaaaagt ttcccataaa
      841 tgaaagcgat agagaaaaat ttgaaaaaca ttacgagaaa attttacgtg aaagtgatgg
      901 taaactgatg cctgaaattt atcgtgaatt gaataaagaa tttttgaaat tgagtggact
      961 ttctgatcct tttgccgaag aaaaacgtaa aagtaatgaa atagcccttg aattatataa
     1021 tgatttaaaa caggttgttg aaaagtctga aaatcctttt cagttggcgt tgcgactcgc
     1081 tattgcaggg aacattatgg attatggtcc cactgcacat tttgatgtac atagcactat
     1141 tgaaaaggct tttactattc aatttgccat tgatcattca gtagaattag aaaaacgaat
     1201 taaaatagct aaaaaaattc tgtatctggg tgacaatgca ggcgaaattg tttttgacaa
     1261 attgtttatt gaaacaatga aacatcccaa tgtaactttt gctgttcgtg gtgctccagc
     1321 actgaatgat gttacactcg aagatgctta ccaggttgga atagacaaag ttgccagagt
     1381 aatttccaat gggaatgatg tagcttctac aattgtaaaa gaatgcagtt ctgaatttca
     1441 ggaaattttc caggaggcag atttaattat ttcaaaaggt cagggaaatc tggaaggatt
     1501 aatatatcaa aatgatccca gaattttctt tttattagta gcaaaatgcg atgtgattgc
     1561 acaattgctt ggtgtagaaa agggaagttt tgtcgtttac aatactcaat taccaaaaaa
     1621 taaaaattta tgacgcaaag tcttattttt actatattgg ctttattttt agggagtgtt
     1681 ggtagcgtat tactggcagg attaattttg ttattaaagg aaaagaggct acagcagatt
     1741 acgcgttttt taacttattt agcaggaggc acattgttag gttctgcttt ggtgggaatg
     1801 attccagaag cactcgctgt attgaaacct gaaattgtac tgaatgtttt attaggagga
     1861 cttgtttttt tctttgtgtt ggaaaaaatc attttatgga gaatttgtgc cgacgcttcc
     1921 tgcgaaagaa atttattggc tgcagctcca atggttttat ttggcgatgc cttccacaat
     1981 gccattgatg gagtgatt
//
LOCUS       DAOTKS010000313         1997 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_45576_length_1997_cov_4.244078, whole genome shotgun sequence.
ACCESSION   DAOTKS010000313 DAOTKS010000000
VERSION     DAOTKS010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45576_length_1997_cov_4.244078"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="P5236_06060"
     CDS             <1..975
                     /locus_tag="P5236_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HRU63963.1"
                     /translation="QSFIPLVIGFGCNVPAILSTRTLKNRKNRILTILIIPFMSCSAR
                     LPVYLLLISAIFSKNQALILFSLYLIGILIAVITALIMKKIAFKEEESPFVMELPPYR
                     IPTLKTINIHMWDKSREYLRKIGNIILVASIIIWAIGYFPRQPEIAKKYEKQILAIQN
                     NTSLNEKEKEKTIFQLQTAKASELQQKSFIGQLGKAIEPVIRPLGFDWKMGVSLLTGF
                     AAKELIISTMGILYQVDTSGQSSVSLQQKIKEEFHTTGKLKGERVFTPLVAYAFMVFI
                     LLYVPCVATIAAMKKEAGWQWAIFGIVYNTAIAWLAAFLIYQIGSLLL"
     gene            complement(1553..1623)
                     /locus_tag="P5236_06065"
     tRNA            complement(1553..1623)
                     /locus_tag="P5236_06065"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1589..1591),aa:Gln,seq:ttg)
     gene            complement(1728..>1997)
                     /locus_tag="P5236_06070"
     CDS             complement(1728..>1997)
                     /locus_tag="P5236_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008778966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRU63964.1"
                     /translation="LQVFDGVPQFHISKEALSQSVKAVDLLTEIATVFPSKSEMRKTT
                     QAGGVMINKERLENPETLIDDSSLIGGKYLVAQRGKKNYFLLIAE"
BASE COUNT          663 a          300 c          372 g          662 t
ORIGIN      
        1 caatcgttta ttccattggt aataggattt ggctgcaatg ttcctgctat tctgtcaacg
       61 cgtacgttaa aaaatagaaa aaaccgaata ttgaccatac ttattatacc gtttatgtcg
      121 tgtagtgctc gattgcctgt ttatttgttg cttatttcag ccattttttc taaaaatcaa
      181 gcactcattt tattttcact ttatttgatt gggattttaa ttgccgtgat aacagcattg
      241 ataatgaaga aaatagcatt taaggaggag gagtcgccct ttgtaatgga attgcctccc
      301 tatcggattc ctactttaaa aaccattaat attcacatgt gggataaaag tcgggaatat
      361 ttgcgaaaaa taggaaatat tattctggta gcttcaatta taatctgggc aattggctat
      421 tttcctcgcc aaccagaaat tgcgaaaaaa tatgagaaac aaattttggc aattcaaaac
      481 aatacttcat taaatgagaa ggaaaaggaa aaaacaattt ttcaacttca aacggctaaa
      541 gcttctgaat tacagcagaa atcttttatt ggacaattgg gaaaagcaat tgaacctgtc
      601 atcagacctt tgggttttga ttggaaaatg ggtgttagtc tgttaacagg atttgcagcc
      661 aaagaattga taattagcac aatgggtatt ctatatcagg ttgacacttc tggtcaaagt
      721 tcagtctcat tacagcaaaa aattaaagaa gaatttcata ccacaggtaa attgaaagga
      781 gaaagagttt ttactccgct ggtggcttat gcttttatgg tatttatttt gctttatgtt
      841 ccttgtgtag ctactattgc agctatgaaa aaagaagcag gctggcaatg ggctattttt
      901 gggattgttt ataatactgc aatagcctgg cttgctgctt ttctaattta tcaaatagga
      961 agtttattgt tataataact gaactaatta ggttgagaat ttgttaaaat gaaattttaa
     1021 ttgaaaatga tgtttcaaat catttcagta atagttattt tatcaacggc gattggttgc
     1081 tgcatatttt ctattacaag gaaaatttct cgcaaagatt ttgattctcc ttgtagcaaa
     1141 tgtaacggtt gtgaatttaa ggaggagata ctaaaaaaaa atcacaaata aaatgtgcca
     1201 aaccaactaa tatccattca ataaaaaact gatttttaag atcgttttac attggtaata
     1261 ccaacttgct ttctatagta aaaaaataat taaaaaatag ctggcagtta ggagtttaga
     1321 gtttcgagat cgaggttggg aatatgaagt aatgaggtac gaattaggag tttcgagttt
     1381 gcggggtacg agaaaaacga agttcgggat gcaagtaaag atattttgca aatgcaggaa
     1441 caaattaata gttctgagtt ttttgagtag gagagaaatt tagcaaaatg tttttgaaaa
     1501 tcttatgtta ataaaacaaa ggagtcaaaa agtaattttc tggctccttg tgttgtccta
     1561 ccaggattcg aacctggact ggctgaacca aaatcagatg tgctaccatt acaccatagg
     1621 acaatcctca ttgcttcgta aaaagcagtg caaaagtaga ataacctttg ctaatttgca
     1681 aatttctttg ctattttttt gtcataattt gtgctgacaa tcagttttta ttcagcaatt
     1741 aacaggaaat aatttttctt tccacgctga gcaacaaggt attttcctcc gataagagac
     1801 gaatcgtcaa tcaaagtttc aggattctct aatcgttcct tgttaatcat tacaccgcca
     1861 gcttgcgtag ttttacgcat ttcgctttta gaaggaaaaa cggttgctat ttccgtcaat
     1921 aagtctacag cttttacact ttgagaaagt gcttcttttg aaatatgaaa ttgaggaacg
     1981 ccatcaaaaa cctgaag
//
LOCUS       DAOTKS010000314         1989 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_45849_length_1989_cov_3.477249, whole genome shotgun sequence.
ACCESSION   DAOTKS010000314 DAOTKS010000000
VERSION     DAOTKS010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45849_length_1989_cov_3.477249"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="P5236_06075"
     CDS             complement(<1..332)
                     /locus_tag="P5236_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRU63965.1"
                     /translation="MNKLHIITPVKDAIDIAFQTIEAILNSEISIDYKYTVYNDFSTA
                     ENTEKLQSLSNEKGFELVNLPELTSHPSPNYLLILQLAQKKALQEKAHLIIVESDVIV
                     EKNTIQNL"
     gene            complement(335..1834)
                     /locus_tag="P5236_06080"
     CDS             complement(335..1834)
                     /locus_tag="P5236_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRU63966.1"
                     /translation="MAQRQMKKLAKDTAIYGLSSIIGKFLNWCLVPLYTYVLPSSADY
                     GIVTNLYAWTALLLVILTYGMETGFFRFANKTEENPNKVYGTTLVSIGTTSLLFAALC
                     VIFSRPMATGMGYSRHPEFIAMLAVIVAMDAFDAIPFAYLRYKNRPIKFAALKLFMIF
                     MNILFNIFFLVLCPIIAKKAPSLIDWFYNPNYGVGYIFVANLISTLSVTLALLPDIRT
                     ANFRFDSDLWKRMLQYSFPLLILGIAGILDQTVDKIIFPFLFEDKAYAASQLGIYGAC
                     FKISMVMMMFTQAFRYAYEPFIFAQYKDKDSKEAYAEAMKYFVIFSLLIFLGMVFYLD
                     IFKFLIRSDYWEGLRVVPIVLFSYLFQGIFFNLSLWYKLTDKTYYGAIISIIGSIVVL
                     TINILFVPVFSYMASAWASFACYFVMMLISYFLGQKYMPIKYDLKKMGVYTLLTLVLY
                     TVSLLISTSSMGLNLVFNTILLIFFVSYLIKHDLPLENIPVVNRLFKKI"
BASE COUNT          764 a          310 c          309 g          606 t
ORIGIN      
        1 aataaatttt gaatagtatt tttttctaca ataacatccg attctacaat gattaaatga
       61 gctttttctt gcaaagcttt tttttgtgct aattgtaaaa ttagcaagta attaggagat
      121 ggatgagagg ttaattccgg aagatttacc aattcaaatc ctttttcatt tgataatgat
      181 tgaagttttt ctgtgttttc agcggtacta aaatcattat aaacagtata tttgtaatca
      241 attgatattt cggaatttaa aatagcttcg atggtttgaa aagcaatatc gattgcatct
      301 tttacaggag ttataatatg taatttattt atattcatat ttttttaaat aatcgattaa
      361 ccactggaat attttccaga ggaagatcat gctttataag atatgaaaca aagaaaataa
      421 gtaaaatagt gttgaatact aaattcaatc ccattgaaga cgtggaaatt agcaaactga
      481 cggtataaag tacaagcgtt aaaagcgtgt aaacccccat ttttttaaga tcatatttta
      541 tcggcatata tttttgtcct aaaaaatagg aaattagcat cataacaaaa taacaagcaa
      601 atgaagccca tgcagaagcc atataactaa atactggaac aaataaaata ttaatggtca
      661 acacaacaat acttccaata attgatataa tagctccgta ataagttttg tcagtcaatt
      721 tataccaaag agatagatta aaaaagatgc cttgaaataa ataggaaaac agaactatag
      781 gtacaacccg tagcccttcc caataatcgc tgcgaataag aaatttaaaa atatctaaat
      841 aaaaaaccat tcccaaaaaa atcagtaatg aaaaaatgac aaaatatttc attgcttcgg
      901 cataagcttc tttgctatct ttatccttat attgagcaaa tatgaatggt tcataggcat
      961 atcgaaatgc ttgggtaaac atcatcatta ccattgaaat tttaaaacag gctccgtaaa
     1021 tgccaagttg cgaagcagca tatgctttgt cttcgaaaag gaaaggaaaa ataattttat
     1081 ccaccgtctg atcaagaatg ccagcaattc ctaatatcag caatggaaaa gaatattgca
     1141 acattctttt ccataagtct gaatcaaagc ggaaattagc tgttcgtata tcgggtaata
     1201 aagccaacgt cactgaaagt gtcgagatta aatttgcgac aaaaatataa cccacgccat
     1261 aattcggatt gtagaaccaa tcaattaagg aaggagcttt tttcgcaata atagggcaaa
     1321 gaaccagaaa aaatatatta aaaagaatat tcataaaaat catgaatagc tttaaagcag
     1381 caaactttat agggcgattt ttatatcgca aataagcaaa aggaatagca tcaaaagcat
     1441 ccattgcaac aatcacggca agcatagcga tgaactccgg atgtctggaa taacccatgc
     1501 cagtagccat gggacgtgaa aaaataacgc ataaagcagc aaaaagtaaa gaggttgttc
     1561 caatcgatac taatgttgtt ccataaactt tattgggatt ttcctcagtt ttattagcaa
     1621 atcgaaaaaa acctgtttcc attccataag tcaaaatcac cagcagtaaa gctgtccagg
     1681 cataaagatt ggtaacaatg ccataatctg ccgaactggg taaaacgtaa gtatataatg
     1741 gaaccaggca ccagtttaga aattttccta taatactgct taacccataa atggcagtat
     1801 cctttgccag ttttttcatt tgcctttgtg ccattttcta atttttttga ttttttaaat
     1861 ggataaattt aattttttcg caaatgattc agaaaatgta agatgtatca ataaaaaatc
     1921 tttagagaat aaataagctg aattcaagtc acaaatgcaa ctttttggtt agacaaattt
     1981 cgtaataaa
//
LOCUS       DAOTKS010000315         1988 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_45883_length_1988_cov_4.576824, whole genome shotgun sequence.
ACCESSION   DAOTKS010000315 DAOTKS010000000
VERSION     DAOTKS010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45883_length_1988_cov_4.576824"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="P5236_06085"
     CDS             complement(<1..404)
                     /locus_tag="P5236_06085"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase
                     N-terminal domain-containing protein"
                     /protein_id="HRU63967.1"
                     /translation="MKKTIILFFSITLLIISAEAKKPKKTERKNQSDITFTNNVDSMS
                     YALGLLVGNDFLKNIKTIPGGKTNVDLIIKGFATAMKGDSAIMSFDSANNYLRDYFTA
                     AQAKDSEERKTAAIAFMEQNKKKEGIITTESG"
     gene            complement(516..>1988)
                     /locus_tag="P5236_06090"
     CDS             complement(516..>1988)
                     /locus_tag="P5236_06090"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRU63968.1"
                     /translation="CNGYFVKIGDTKDNVSLWLQEGIKKTKLIDGVEKRTDGNPVEIK
                     IKVTRDENGNFSLYSKLPSENDYFLEGTVQNLAIKQSLYFGLMYANTSTTGSAYYFDD
                     ILVRGNKAVDMESPQWEQLTLLPPNQLKLQFSEPMDFLQATFTVNEEIGTPSNIEISV
                     DKTVVILTFENEFQRGKIYTLEINEATDLAGNPLLNTTKITGITEPIEMGDLVFNEIM
                     FDNPLNSTEYLEIFNRSEKLLDVSGIVFTTRKKDGNLNTGHEIPKGTLLKPAGCLAVC
                     PNADSLLNYYHYPPESYVVTTSWSNLNNESATLVLANNNKDTIYDELTYNVKWHHIMI
                     KNPKGVSLEKINPSLPSQDPQSWHSAAFEVNYGTPGYKNSQYREIDISMEPERFIWAD
                     PEAFSPDNDGVEDVCLIHYKTATNGFVATATIFNAVGVKVCQLLSNVLLASEGFFLWN
                     GKTSDGKIANPGIYVLYFEMIQPDLGIKKQAKIPIVVSLR"
BASE COUNT          635 a          340 c          309 g          704 t
ORIGIN      
        1 aagccgcttt ctgtagttat tattccttct tttttcttgt tttgttccat aaaggcaatc
       61 gctgcagttt ttctctcctc agaatctttg gcttgagctg cagtaaaata atctctcaaa
      121 tagttgttgg cagaatcaaa agacataata gctgaatcgc ctttcatagc tgtagcaaat
      181 cctttgatta tcaaatcaac gttagtttta ccaccaggaa tagtttttat attttttaaa
      241 aaatcgttcc ctaccagcag acctaaggca taactcattg aatctacatt attggtaaaa
      301 gttatatctg attgattttt cctctcagtt ttcttaggtt tttttgcttc agcggatata
      361 atcaataatg tgatagaaaa aaataaaatg atggttttct tcattgataa aaaatctaaa
      421 tgtttttata catgttaact taaaatgaac aagtgattta taagcggtgc aaagatagtt
      481 aatttattac acaataacat gttaatattt caaaattatc gtagtgagac tacaatagga
      541 attttagcct gtttttttat tcctaagtca ggttgaatca tttcaaaata aagcacataa
      601 atgcctggat tggcaatttt gccatcgctt gttttgccgt tccagagaaa aaatccttcg
      661 gaagcgagca gtacatttga aagcaattga catactttta caccaacagc attaaaaatt
      721 gttgcagtag caacaaaacc attcgttgct gttttgtaat gaataagaca tacatcttcc
      781 acaccatcat tgtcaggtga aaaagcttct gggtctgccc atataaatct ttcaggttcc
      841 attgaaatat caatttctct atattgagaa tttttataac ctggtgttcc ataatttact
      901 tcaaaagctg ctgaatgcca tgattgtgga tcctgtgaag gaagagaggg attaattttt
      961 tccaatgaaa caccttttgg attttttatc ataatgtgat gccattttac attataggtc
     1021 aattcatcat aaatcgtatc cttgttattg ttagctaata ccaaagtagc actttcgttg
     1081 ttaagatttg accaactggt agtaacaaca taactttcag gtggataatg gtaataattt
     1141 agcaatgaat cagcattggg acaaacagct aagcaaccag caggttttaa aagcgttcct
     1201 ttaggaattt cgtgtcctgt attaagattc ccatcttttt ttcgagtagt aaatactata
     1261 cctgaaacat ccagcaattt ttcagaacga ttgaaaattt caagatactc cgttgaattt
     1321 agaggattat caaacatgat ttcattgaaa accagatcac ccatttcaat tggttcagtt
     1381 atgcctgtta tttttgttgt atttaaaaga ggatttcctg ctaaatcagt tgcctcgtta
     1441 atttccaaag tatagatttt accacgttga aattcatttt caaaagtgag tattacaacg
     1501 gttttgtcga cagatatttc aatattagaa ggtgttccta tttcttcatt tacagtaaaa
     1561 gttgcctgca aaaaatccat cggctcagaa aactgtaatt ttaactgatt aggaggtaaa
     1621 agagtaagtt gttcccattg aggtgattcc atatcaacag ctttattgcc cctaaccaaa
     1681 atatcgtcaa aataataagc tgaaccagtt gtgctggtat tagcatacat taatccgaaa
     1741 taaagacttt gtttgatagc taaattctga acggttcctt ccaaaaagta gtcattttca
     1801 gatggcaact tgctgtatag tgaaaaatta ccattctcgt cacgagtaac tttaattttt
     1861 atttcaacag gatttccgtc tgtacgtttt tcaactccat caattaattt agtctttttt
     1921 attccctctt gtagccataa tgaaacatta tctttggtat caccaatttt aacaaaatag
     1981 ccattaca
//
LOCUS       DAOTKS010000316         1987 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_45919_length_1987_cov_4.257764, whole genome shotgun sequence.
ACCESSION   DAOTKS010000316 DAOTKS010000000
VERSION     DAOTKS010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45919_length_1987_cov_4.257764"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="P5236_06095"
     CDS             <1..941
                     /locus_tag="P5236_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HRU63969.1"
                     /translation="DYYKILGVNKNASQEEIKKAYRKLAVKYHPDKNPGNKQAEEQFK
                     LINEAYEVLGDPEKRKKYDDLGANWNRFSQEGSYSGGSPFEGFGHQGGQQFFYEGDLN
                     DLFGGGGGSGFSDFFERFFGRGAAGRGASSGFGRGFRQAEVRGQDYEVEIVLTLEEAY
                     HGTSRIIQLDNEKIRIKIKPGAYDGQVLRIKGKGGKGSSPANNGDLLVKIKILPHPVF
                     ERIGDDLKIVHQISLFDAILGGKTIVDTLDGKIKIDIPEGTQNNKILRIKGKGMPIYG
                     KTNEYGDLLVQLQIKIPDNLTPAQKNMFKQIKDTIQ"
     gene            962..1315
                     /locus_tag="P5236_06100"
     CDS             962..1315
                     /locus_tag="P5236_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63970.1"
                     /translation="MNGIKKFSILEDEMLNSLVEAIYDSELIYSSIDLYSLGIESPNE
                     IDAAVMSAIQTIRRADLSPQQHFKYIYLTDIKSGNILHDWRMSKMGFLLTLLNANTTN
                     SLINQLKIELINRIV"
     gene            1536..1961
                     /gene="ssb"
                     /locus_tag="P5236_06105"
     CDS             1536..1961
                     /gene="ssb"
                     /locus_tag="P5236_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRU63971.1"
                     /translation="MSVNKVILIGNVGKDPEIRHLDKNVTVAKFPLATSERGYTMQNG
                     TQVPERTEWHNIVAWRGLAEIVEKFVKKGSQLYVEGKIQSRSWEKDGIKRYITEIYAE
                     NIQLLGKRSEAAEEIATSSTVESVQIPPELEEIDDDLPF"
BASE COUNT          767 a          240 c          383 g          597 t
ORIGIN      
        1 aagattatta taaaatactg ggagtaaata aaaatgcttc tcaagaagaa ataaaaaaag
       61 cataccgaaa attagctgta aaatatcatc ctgataaaaa tccagggaat aagcaagcag
      121 aggaacaatt taagcttatt aacgaagctt atgaggtatt gggcgatcct gaaaaaagaa
      181 agaaatatga tgatttagga gcaaattgga atcgtttctc gcaggaaggt agctattcag
      241 gaggaagtcc atttgaagga tttggtcatc aaggaggtca acagtttttt tatgaaggag
      301 acttgaacga tttatttggt ggcggtgggg gttctggctt ttcagacttc tttgagcgat
      361 tttttgggag aggagccgct ggtagagggg cttcaagtgg atttggcaga ggatttagac
      421 aagcagaagt aagagggcag gattatgaag ttgaaatagt tttaactctt gaagaagctt
      481 atcatggaac aagtagaatt attcaactgg ataatgaaaa aatcagaatc aaaataaaac
      541 caggggctta tgatggacaa gttctaagaa ttaaaggtaa aggtgggaaa ggaagtagtc
      601 ctgcaaataa cggagatttg ttggtaaaaa taaaaatatt gccacatcca gtatttgaaa
      661 gaattggtga cgatcttaaa atagttcatc aaattagttt gtttgatgct attttaggag
      721 gaaaaactat tgttgatact ttagatggga aaataaaaat tgacatacca gaaggtactc
      781 aaaataataa aatactccgc atcaaaggga aggggatgcc tatttacgga aaaacaaatg
      841 aatatggaga tttgcttgtt caacttcaaa taaagatacc tgataatttg acccctgctc
      901 aaaaaaatat gtttaaacag ataaaagata cgattcaata attcattaaa aaatttcaaa
      961 aatgaatgga ataaaaaaat tttctatttt agaagatgaa atgttaaact cattggtaga
     1021 agctatttat gatagtgaat taatatattc ctccatagat ttatattcat taggaattga
     1081 atcgcctaat gaaattgatg ctgcagtaat gagtgctatt caaaccattc gaagagcaga
     1141 tttatcaccg cagcaacatt tcaaatacat ttatttaact gatataaaaa gcggtaatat
     1201 attacatgat tggagaatga gtaaaatggg ttttttattg accttgttaa atgctaatac
     1261 tactaattca ttgattaatc aacttaaaat tgaattgatt aatcgaattg tataataatc
     1321 agttgttttg aaaataatat ttttggaata aaaataaaac ctttgtttat ctgaaaaatg
     1381 aaagataagc tttttaaaag gcactattcg gatataaaga agtaaagaat taaagttttt
     1441 ttaatttttt ccctatttaa ttttgaaaaa cataaaagaa tttttatttt taccaaaatt
     1501 atttcaaaat aaaaaactcc ttaaacttta aagttatgtc tgtaaacaaa gttattttga
     1561 tagggaacgt agggaaagac ccagaaatac gtcatttaga taagaatgta acggttgcga
     1621 aatttcctct tgctacaagc gaacgaggtt atactatgca aaatggcaca caagtaccag
     1681 aaagaactga atggcataat attgtagctt ggcgaggatt ggcagaaatt gtagaaaaat
     1741 ttgtaaaaaa aggtagtcaa ctctatgttg aaggtaaaat tcagagccgt tcgtgggaaa
     1801 aagatggcat aaaacgttat ataactgaaa tttacgcaga aaacattcaa ttattgggca
     1861 aacgttctga agccgcagaa gaaattgcta cttcttcaac ggtagagtct gtgcaaattc
     1921 cacctgaatt agaagaaata gacgatgatc ttcctttttg atcttccttt tataagtttt
     1981 aaaaaag
//
LOCUS       DAOTKS010000317         1977 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_46246_length_1977_cov_10.710198, whole genome shotgun
            sequence.
ACCESSION   DAOTKS010000317 DAOTKS010000000
VERSION     DAOTKS010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46246_length_1977_cov_10.710198"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..973)
                     /locus_tag="P5236_06110"
     CDS             complement(11..973)
                     /locus_tag="P5236_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63972.1"
                     /translation="MDEKNTKEINLLDLIKMFNAWVVKVGKSFINFILYLLKAGYRKK
                     YLTLTLIVLCFAIGEYMARPSNRQYAAGAMASLYGVDAFTMKEIAKQLEQSRANNRYT
                     SLGTKLSLPDSVAKNIVAFNSYFVIDYLNDLTPDMVDFANNHSLEDTLNVRMKDKIYF
                     RLITRNISQVPQVEKAILNFFNSQPIVQLKYTARRNELEGKIDISAAEMQRLDSLAKL
                     TYFKDTEKQLKWSKDRLIVGEQQKQLFYGEMLLLQKVKAEAESELLTYKQPVELPSGF
                     VVNPFPLNGRLKYGIYGILIGLGLGLALAACLEQLQKVVQFFRK"
     gene            complement(1016..1699)
                     /locus_tag="P5236_06115"
     CDS             complement(1016..1699)
                     /locus_tag="P5236_06115"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRU63973.1"
                     /translation="MTSDSENELEKQRWKFAIFVPLIVCILWILCFAIEHGMGWDFHK
                     AGIYPRKLENIWSIFTYTFIHADWKHLMNNALSFFILGICLYYFYSEVANRVLIVSAV
                     LSGFILWCIGRESYHIGASGIIYSLAFFLFFSGIIRKHIPLIAISLIVVFIYGSMVWH
                     LFPWLPDDPVSWEGHLSGAVTGIFLAFVYRHRGPQPPKPEWEEEDDNKNEGEDYWEES
                     YDIEQWKEE"
BASE COUNT          667 a          382 c          292 g          636 t
ORIGIN      
        1 aaaggtattt ttattttcta aaaaattgaa ccaccttttg caattgttcg aggcaagcgg
       61 caagcgccaa acctaagccc aaacctatta aaataccata aattccgtat tttaatcttc
      121 cattcaaagg aaaaggattg actacaaatc ctgaaggtaa ttcaacaggc tgtttataag
      181 tcagtaattc agattctgct tcagctttta ccttttgaag caacaacatt tctccataaa
      241 atagttgttt ttgctgttcg cccacaatca aacggtcttt cgaccatttc aactgttttt
      301 ctgtatcttt aaaataggta agtttagcca agctgtccaa gcgttgcatc tcagcagcag
      361 aaatatcaat ttttccttcc aattcatttc ttcgagctgt atatttaagc tgaacaattg
      421 gttgtgaatt aaagaaattt aaaattgcct tttcaacttg aggaacttga gaaatatttc
      481 gggtaataag gcgaaaatat attttatctt tcattcgaac atttaaggtg tcctctaatg
      541 aatggttatt ggcaaaatca accatatctg gagttaaatc attcagataa tcaattacaa
      601 aataagaatt aaatgctacg atattttttg caacgctatc cggcaatgac aatttggtgc
      661 ccagtgaagt gtaccggtta ttggctctcg attgctccaa ctgttttgct atttccttca
      721 tggtaaaagc atctacacca tacagcgaag ccatagcgcc ggcggcatac tgccggttac
      781 tgggacgagc catgtattcg cctatggcaa aacacaatac aatgagagtc agggtcaaat
      841 atttttttcg ataacctgcc ttcagcagat acaaaatgaa gttgataaaa gatttaccaa
      901 cttttacaac ccaagcattg aacattttta tcaaatccag caaattaatt tctttagtat
      961 ttttctcgtc catattgatt gaatatttat ttgtttttca aaatcgattg cctaattatt
     1021 cttctttcca ttgctctata tcataacttt cttcccaata atcttcacct tcatttttat
     1081 tatcatcttc ttcttcccat tctggtttgg gaggttgtgg tcctctgtgc ctgtacacaa
     1141 aagccaaaaa aattcctgtt acggctcccg ataaatgtcc ttcccacgaa accggatcgt
     1201 cgggcaacca cgggaaaaga tgccaaacca tgctgccata aatgaataca acaattaacg
     1261 aaatggctat caatggaata tgtttcctta taattccgct gaaaaacaga aaaaacgcca
     1321 atgaataaat gatgccactt gcaccaatgt gataactttc gcgtcctata caccataaaa
     1381 taaagccact caatacagct gaaacaatca gcacacggtt ggcaacttca ctgtaaaaat
     1441 aatataggca aattcccaaa ataaaaaatg aaagagcatt gttcattaag tgcttccaat
     1501 cggcatgaat gaatgtatat gtaaatatac tccaaatatt ttccaatttt ctgggataaa
     1561 taccagcttt gtgaaaatcc caacccatgc catgttctat cgcaaaacac aaaatccaca
     1621 aaatacatac gattaaagga acaaaaatgg caaatttcca tcgttgcttc tccaattcat
     1681 tttcgctgtc gctcgtcatt ccgtagcact attttctaaa taacgtcttc caaacactaa
     1741 aaattacatc aaaggtaaaa atttttttat ttgggtagta atttttatgc tatcaaatga
     1801 agttttgata agataaaata ttcagttttt gccggaaatt atataacatt ttgataaaca
     1861 ataaattgcg agtatgaatc aagtttgtaa ttaagtggaa ttaaaaaaag tattttacag
     1921 acattgaaaa aaatatcaac tttctttttt taattggaaa ttttgaataa attgcaa
//
LOCUS       DAOTKS010000318         1973 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_46373_length_1973_cov_4.063087, whole genome shotgun sequence.
ACCESSION   DAOTKS010000318 DAOTKS010000000
VERSION     DAOTKS010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46373_length_1973_cov_4.063087"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="P5236_06120"
     CDS             <1..204
                     /locus_tag="P5236_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HRU63974.1"
                     /translation="AQFGKPIVSTSANISGKATPLNFSEISDEIKSAVDYIVKYRQKE
                     TLEPKPSSIIKIEKGNVIQIIRQ"
     gene            284..1684
                     /locus_tag="P5236_06125"
     CDS             284..1684
                     /locus_tag="P5236_06125"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRU63975.1"
                     /translation="MNKKKLLLTYLFFDLLASLIVWILFMLFRKIVNDSAIFENVQIF
                     FPNFNYFSSLVLFPFCCIIVHYLSGFYLYPEKMTGIKIFFSTFAASIIISITIFFVLL
                     LDDIVVSYKYYYYSLLVLFGLLFVFTYFPRLMIYLNVQKNYKTKRWTIPTLIIGTGQN
                     AKKLADELVKRNQKNTLVGFINVDHEVSVPKEKILGNLRNIRNIIEENHIQETIIALE
                     NASEQQLFSIINLLYQYNVDIKFTPRLYEILTGSAKIKDLNVSPLVNITDINMPDWEQ
                     SVKRVFDIVFSLFSLIILFPVMLFFMIWIKLDSKGPIFYLQERIGRYGRPFKIIKFRT
                     MYEGAENGVPQLSNPNDSRVTRVGKILRRYRIDEIPQFINVLLGDMSIVGPRPERRYY
                     IQQIIQEAPYYCLLYKIRPGLTSWGPIRIGYSDTIEKMIERLNYDIIYMDNMSLITDL
                     RILLMTIKIIFKGSGV"
BASE COUNT          662 a          297 c          306 g          708 t
ORIGIN      
        1 gctcaatttg ggaaacccat tgtctccact tcagccaaca ttagtggtaa agcaactcct
       61 ttgaattttt ctgaaattag tgacgaaata aaatcggctg tagattatat tgttaaatac
      121 cgtcaaaaag agactttaga accaaaaccc tcttcaatta ttaaaattga aaaaggaaat
      181 gttattcaaa tcattcgtca ataatttttt tgaatttttt caggcaaaca agctgttttt
      241 gaaataaaaa taaaaagtta ttaactcata ttgatttaat attgtgaaca aaaagaagtt
      301 acttttaact tacctttttt ttgatctact tgcttcttta atagtatgga ttttatttat
      361 gctattccga aagattgtga atgattcagc aatatttgaa aatgtccaaa tttttttccc
      421 aaatttcaat tatttttcta gcttagtttt gttccctttt tgttgtatta tagtgcacta
      481 tctatcagga ttttacctgt atcctgaaaa aatgactggt attaaaatct ttttttcaac
      541 ttttgctgcc tcgattatta tctctatcac catttttttt gttctactgc tcgatgatat
      601 tgtggtttcc tataaatatt actattattc tctgttagtt ttgttcggat tgctttttgt
      661 ttttacttat ttcccacgat tgatgattta cttaaatgtt cagaaaaatt ataaaacaaa
      721 acgctggact atccctactt taataattgg cacgggacaa aatgcaaaaa aactggctga
      781 cgaattggta aaaagaaatc aaaaaaacac tttggtagga tttatcaatg ttgatcatga
      841 agtgagtgta cctaaagaaa aaatattggg aaatctccga aacattagaa atattattga
      901 agaaaaccat atacaggaaa ctatcattgc tctggaaaac gcaagtgaac aacaattgtt
      961 ttccatcatt aatcttttgt accaatataa tgttgatata aaatttacgc cacgattata
     1021 tgaaattctg actggaagtg caaaaataaa ggacttaaac gttagtccgc tggtaaatat
     1081 tacggatatt aatatgccag attgggaaca aagtgtaaaa agagttttcg atattgtgtt
     1141 ttctcttttt tcgctgatta ttctttttcc tgttatgctt ttttttatga tatggataaa
     1201 acttgattca aaaggtccga tattttactt acaagaacgt atagggagat acggacgacc
     1261 tttcaaaatt attaaattcc gaactatgta cgaaggagca gaaaatggtg ttccacaact
     1321 gagtaatccg aatgattcac gggtaacgcg agtagggaaa attttgcgtc gttatcgtat
     1381 tgatgaaata cctcaattta tcaatgttct attaggtgat atgagtattg tcggtccaag
     1441 acctgaacgc cgttactaca ttcagcaaat tattcaggaa gccccttatt attgtctttt
     1501 atacaaaatt cgccctggac ttacttcctg gggaccaatt cgcattggtt attccgacac
     1561 gatcgaaaaa atgatcgaac ggttaaatta tgatattatt tacatggata acatgtcact
     1621 gattactgat ttacgaatat tactaatgac tataaaaatc atttttaaag gatcaggtgt
     1681 ctgatacaag ttgcgtttta caacggatct tccttccagg atcttgaaaa gctattataa
     1741 aaatcgctac caatggagga aaatgtgatt tttagttttt cttttgtacg caattatgta
     1801 tagaaaggaa aattcattaa aaatagacaa attttaaaca atttaccaca taaattatta
     1861 acaatcagct tattaaattg ttaacaacta cttattttta aaatttttta tacggcgact
     1921 tgctaagttc aacatagaag tgcgaattta gcagagatgt tttgcatgat ttt
//
LOCUS       DAOTKS010000319         1971 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_46449_length_1971_cov_1.954071, whole genome shotgun sequence.
ACCESSION   DAOTKS010000319 DAOTKS010000000
VERSION     DAOTKS010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46449_length_1971_cov_1.954071"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="P5236_06130"
     CDS             <1..330
                     /locus_tag="P5236_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1793 domain-containing protein"
                     /protein_id="HRU63976.1"
                     /translation="NIFDTKIAEKEIAYYLTKQNPFGLPLDNRKTYTKSDWIMWSAIL
                     AENTNDFNALVDRVYKYANETPTRIPLSDWHETIDGTSVGFRARSVVGGYFMKMLEDK
                     IQSIQYK"
     gene            446..>1971
                     /locus_tag="P5236_06135"
     CDS             446..>1971
                     /locus_tag="P5236_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HRU63977.1"
                     /translation="MTVMLFSFEVYSQENINISIVDRPEIQVVNKNYVQNRAPLLPLH
                     CIKLPVGDVKPEGWLLEMLYRQKNGLNGHLGEISAWLDKENNAWLGTGTDYSWEEVPY
                     WLKGYGNMAYILGDEKMIEETKMWIEAVFKSQREDGYFGPYIEKNGKPDLWGNMIMLW
                     CLQSYYEYSGDQRVIDLMGRYFKWELALPEDQFLKDYWENSRGGDNLLSVYWLYNLTG
                     DKFLLDLAEKIHRNTANWRQRSTLPNWHNVNIAQSFREPATWYLLSKDSADLISTYNV
                     FHLIRNTFGQVPGGMFGADENARIGYIDPRQATETCGFVEQMASDEWLLRMTGDPFWA
                     EHCENVAFNSFPASMMPDMKALRYLTAPNQVISDSKDHSPGIQNSGPFLAMNPFSSRC
                     CQHNHGQGWPYFIEHLIMASPDNGIIAAIYGACKATVKVADGKTIEIIESTNYPFEEK
                     IKFTIHSSQKVPFPLYLRIPSWADKPFIKLNGKLLNVPLVNGMYLKIEREWITNDEVE
                     "
BASE COUNT          619 a          297 c          445 g          610 t
ORIGIN      
        1 aatatcttcg atacgaaaat tgccgaaaag gagattgcct actatcttac caaacaaaat
       61 cctttcggct tgcctttgga taatcgaaag acctacacaa aatctgactg gattatgtgg
      121 tcagcgattt tggcagaaaa tactaacgat tttaacgcat tggtggatcg ggtatataaa
      181 tatgcgaatg aaacgccaac gcgtattccg ttgagtgatt ggcacgaaac cattgacggt
      241 accagtgtgg gattcagggc acgttcagta gtaggaggat actttatgaa gatgctggag
      301 gataaaattc aatccattca atataagtaa attcttttta tatttgtttt ctccaaataa
      361 tgggtttttt taagaaaaaa gtagaaaagg tatttaatag taaattatga gaacaataaa
      421 aggtgtattt gtgaaaccta ttgtaatgac tgtgatgttg ttctcttttg aagtatattc
      481 tcaagagaac ataaatatta gcattgtaga tcgacctgag attcaagtcg tcaataaaaa
      541 ttatgtgcaa aaccgagcac cactgcttcc attgcattgc ataaaattgc ccgtaggtga
      601 cgtgaaaccg gagggttggc ttttggaaat gctttatcgt caaaaaaatg gactaaatgg
      661 tcatttgggc gaaataagcg cttggcttga taaagaaaat aatgcatggc tggggacggg
      721 aactgattac agttgggagg aagtacccta ttggttgaaa ggttatggta atatggcgta
      781 tattttgggt gatgaaaaaa tgattgagga aacaaaaatg tggatcgaag ccgttttcaa
      841 aagtcaacgt gaagatggtt atttcggtcc gtatattgag aaaaacggga aaccggatct
      901 gtggggaaac atgatcatgc tttggtgttt acaatcttat tatgaatatt caggagatca
      961 acgtgtgatt gatttgatgg gacgttattt taaatgggag ttggctttac cggaggatca
     1021 gtttttgaaa gattactggg aaaatagtcg tggaggagat aatttgttaa gcgtatattg
     1081 gctttataac ctaacgggtg ataaattttt gttggatttg gctgaaaaga ttcataggaa
     1141 tacggctaat tggcgtcaac gttcaacact tccaaattgg cacaacgtga atattgcgca
     1201 gagttttcgt gagcctgcaa catggtactt gctttcaaaa gattcggcag acttaatttc
     1261 cacttataat gtgtttcatt tgattcgaaa tacatttggc caagttcctg gcggtatgtt
     1321 tggagctgat gaaaatgcaa gaataggtta tatcgatcca cgtcaggcaa ctgaaacttg
     1381 tggttttgtt gaacaaatgg cttctgatga atggttacta agaatgactg gtgatccttt
     1441 ctgggcggag cattgtgaaa atgttgcatt caactcattt cctgcttcta tgatgcctga
     1501 catgaaagct ctgcgttatc ttacagctcc taatcaggta ataagtgatt ctaaagatca
     1561 tagtccgggt attcaaaaca gcggtccatt tctcgcaatg aatcctttta gcagtcgttg
     1621 ctgtcagcat aatcatgggc agggatggcc ttattttatc gaacatctga ttatggcatc
     1681 gcctgataac ggtattattg cggctattta tggtgcctgc aaggcaacag tgaaagtagc
     1741 ggatggtaaa acgatagaaa taatagaatc gacgaattat ccttttgagg aaaaaattaa
     1801 atttaccatt cactcctctc aaaaagtacc atttccttta tatttaagaa ttccttcatg
     1861 ggctgacaaa ccctttatta agttgaatgg aaaattgttg aatgttcctt tagtaaatgg
     1921 tatgtatctc aaaattgaaa gggaatggat aacaaatgat gaagttgaat t
//
LOCUS       DAOTKS010000320         1969 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_46499_length_1969_cov_4.200627, whole genome shotgun sequence.
ACCESSION   DAOTKS010000320 DAOTKS010000000
VERSION     DAOTKS010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46499_length_1969_cov_4.200627"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1883)
                     /locus_tag="P5236_06140"
     CDS             complement(<1..1883)
                     /locus_tag="P5236_06140"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HRU63978.1"
                     /translation="MTKITREEALLYHSQGKPGKIEIVPTKPYSSQRDLSLAYSPGVA
                     EPCLEIKKSEDAVYQYTNKGNLVAVISNGTAVLGLGDIGPLAGKPVMEGKALLFKIFA
                     GIDVFDIEVKEKDPEKFIQIVKAISPTFGGINLEDIKAPECFEIEEKLKQELDIPIMH
                     DDQHGTAIISGAGLLNALEIAGKKIGEVKIVINGAGAAAISCTKLYIKLGAKPENIIM
                     LDSKGIINKQRPDLTEIKKPFATDKNIHSLEEAIKGADVFLGLSVNGILSIEMVRSMN
                     ENPIIFALANPDPEISYEDAMNAREDIIFATGRSDYPNQINNVLGFPYIFRGALDVRA
                     TCINEEMKLAAVYALADLAKKPVPDVVNAAYNLKKLSFGRDYIIPKPLDPRLLTVVAP
                     AVAHAAMESGVARQKITDWEAYNIKLRELMGGYGDKMLRRFYDMARQNPKRVVFSEAN
                     HINMLKAAEMAYNEGICYPILLGNEEKIEKIANEYNIDLTGMEIINLRHDREEQRRIS
                     YAEQLANKLQRDGMTFDEAYEKMYERNYFGMMMVERGEADALITGVYTKYADSIQAAK
                     DIIGIREGSKNIAALHIMNTKKGTFFLADTLFNRHPSTETLLDIAKLTYRTVKLFAHE
                     PVMA"
BASE COUNT          608 a          410 c          298 g          653 t
ORIGIN      
        1 gccattactg gttcatgagc aaaaagtttt acagttcgat aagtcaattt tgcaatatcc
       61 agtagtgtct cagtagaagg atggcgatta aagagtgtat cagccaggaa aaaagttcct
      121 tttttggtat tcataatatg aagagctgca atattttttg atccttcacg aatgccaata
      181 atatctttag ctgcctggat agaatcagca tatttagtat aaacgcctgt gatcaaagca
      241 tcggcttcgc ctctttcaac catcatcatt ccaaaataat tacgttcata cattttttca
      301 taagcttcgt caaaagtcat cccatcgcgt tgtagcttat tggcaagttg ctcagcataa
      361 gaaattcttc gttgttcttc cctgtcatga cgcaaattga taatttccat gccagtaaga
      421 tctatgttat attcattagc aattttttca attttttctt cattgcctaa taggattgga
      481 taacaaattc cttcattata agccatttca gcggctttca acatatttat atgattggct
      541 tctgaaaaca ccactctttt gggattttga cgagccatat cataaaaacg tcgcaacatt
      601 ttatctccat aaccacccat taattcacgc aacttaatat tataagcttc ccaatctgta
      661 attttttgac gtgccactcc cgattccata gctgcatgtg ctacagcagg agcaactacg
      721 gttaacaatc gtggatcaag cggtttcgga ataatataat ctctgccaaa acttaatttt
      781 ttcaaattgt aagctgcatt tactacatca ggcactggct tttttgccag atccgctaag
      841 gcataaacgg ctgccaattt catttcttca ttgatacaag ttgctcgtac atccaaagca
      901 ccacgaaaaa tatacggaaa tcccagtaca ttattaattt gattagggta atcggatctt
      961 cccgtagcaa aaatgatatc ttcacgagca ttcattgcat cttcataaga aatttcaggg
     1021 tcaggattgg caagagcaaa aatgatagga ttttcattca tggagcgaac catttcaatg
     1081 gacaaaattc catttaccga taatcccaga aaaacatcag ctccctttat tgcttcttct
     1141 aaagaatgaa tatttttatc agtggcaaat ggttttttta tttccgtcaa atcaggacgt
     1201 tgcttgttga taatcccctt agaatcaagc ataattatat tttcaggttt tgcacccagc
     1261 ttaatataca atttggtaca cgaaattgct gctgctcccg ctccattgat cacgattttt
     1321 acctctccaa tctttttccc agcaatttct aatgcattca acaatcctgc cccagaaatg
     1381 atagctgtac catgctgatc atcgtgcata ataggaatat ctaattcctg ttttaatttc
     1441 tcttcaattt caaaacattc aggtgcttta atgtcttcta aattgatacc accaaaagta
     1501 ggcgaaatag cttttacaat ctgaataaat ttttctggat ctttttcctt tacttcaata
     1561 tcaaaaacat caataccagc aaaaatttta aacaataatg ctttcccctc catcacaggt
     1621 ttccctgcca gaggaccaat atctcccaat ccaagaacag ccgttccatt ggaaataact
     1681 gccaccaaat ttcccttatt ggtgtattgg taaacagcat cttcactctt tttaatttcc
     1741 agacacggtt ctgcaactcc cggcgaatac gctagtgaca aatcacgctg cgagctgtaa
     1801 ggtttggtag gaacaatttc aattttaccc ggctttcctt gtgaatgata aagaagagct
     1861 tcttctctgg ttattttagt catataataa atataaattg catttaaatt aaaaagtgaa
     1921 agagaaataa agacttattt agataattgt agcaaaatta caatttttt
//
LOCUS       DAOTKS010000321         1967 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_46565_length_1967_cov_3.666841, whole genome shotgun sequence.
ACCESSION   DAOTKS010000321 DAOTKS010000000
VERSION     DAOTKS010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46565_length_1967_cov_3.666841"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(353..>1967)
                     /gene="polA"
                     /locus_tag="P5236_06145"
     CDS             complement(353..>1967)
                     /gene="polA"
                     /locus_tag="P5236_06145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HRU63979.1"
                     /translation="PSDRKEAAIILKEFKAFFQSEQIEKIGQNLKFDYLMLSMYNIEL
                     RGKLFDTMIAHYLLQPELRHSMDYLAEIYLHYRTIHYEDLVGPKGKNQADIRNIEIAK
                     LCEYACEDADITFRLKQILEKEILENGLEKLFYDIEMPLMRVLAIMEQTGVRIDTEVL
                     QQSSKILTNEMLKIEKEIYQMVGFEFNVSSPSQVGEVLFEHLRLDDNAKKTKTGQYST
                     SEDILEKLRNKHPVVGKILEYRNIKKLLSTYIDALPKLINPQTGKIHTTYNQINTATG
                     RLSSSNPNLQNIPIRDDIGKEIRRAFIPEENSLFFSADYSQIELRIMAHLSNDEAMVE
                     AFKKGQDIHAATASKIYKVPLEEVTAEMRRNAKTANFGIIYGISAFGLAERLGISRTE
                     AKNFIDEYFNTYPGVKSYIDNIIQKAREKGYVETLLGRKRFLPDINSQNSVVRGFAER
                     NAINAPIQGSAADIIKIAMVNIQHRLEQENLQTKMIIQVHDELDFTVPKEEVETVREL
                     VIYEMEHTFQLNVPLIVDCGIGNNWLEAH"
BASE COUNT          624 a          343 c          288 g          712 t
ORIGIN      
        1 aatattggat gctgtaaata ttcttttata gattcattat tccattcttg caaatccacc
       61 attgattttg ataaattgat tcactatttc tcgttaaaaa ccagataaat aattattttt
      121 gtgataataa gattaccaag tgcaaaagta gagcaaaaat taaaaataaa aaaattaaga
      181 aaaatcttgt tgtactgaat aaatgaatgg ttacaatttg agaatctcaa actaaaaata
      241 gtctcgatga aacaaaagaa atatggtgct ttttaaagtt tcatcccatt atgaatgtat
      301 tttccaatga agctaaagac gctttgttaa aggagcaaaa atatcttaat ttctaatgag
      361 cttccagcca gttattgcct atcccacaat ccacaatcaa cggcacgttt aattgaaaag
      421 tatgttccat ttcgtaaatc acaagttctc gcactgtttc aacttcttct tttggtacag
      481 taaagtctaa ttcatcatgc acttgaataa tcatttttgt ttgtaaattt tcctgctcaa
      541 gtcggtgttg aatgttgacc atagctattt taataatatc agcagcagat ccttgaatag
      601 gagcatttat agcatttcgt tcggcaaagc ctctcactac actgttttgg gaatttatgt
      661 caggtaaaaa tcgtttcctt cccaacaagg tttctacgta acctttttct ctagcttttt
      721 gaataatatt atctatataa ctttttaccc caggataagt attaaagtat tcatcgataa
      781 aatttttagc ttctgtccgt gaaataccta atcgttcagc cagaccaaaa gctgatattc
      841 cataaatgat accaaaatta gcagtttttg catttcgcct catttcagca gtaacttctt
      901 caagagggac tttataaatt ttcgatgctg ttgcagcatg aatatcctga ccttttttaa
      961 aagcctctac cattgcctca tcattgctta aatgagccat gatgcgtaat tcaatttgtg
     1021 aatagtcggc actaaaaaaa aggctatttt cttcgggaat aaaagctctt ctaatttctt
     1081 taccaatatc atcgcgaata ggaatatttt gtaaattggg attgctggaa cttagtctgc
     1141 cagtagctgt attaatttga ttataagtag tgtgtatttt acctgtttga gggttgatta
     1201 atttgggaag agcgtcgata taagtactta acagtttttt aatgtttctg tattccaata
     1261 tcttacctac tactggatgc ttattacgca atttttcaag tatatcttca gaagtagaat
     1321 attgacctgt ctttgttttt ttggcattgt catctaaacg caaatgttca aacagtacct
     1381 ctccaacttg gctgggtgaa ctaacattaa attcaaaacc aaccatctga taaatttctt
     1441 tttctatttt caacatttca ttggttagaa tttttgaact ttgctgtaac acttctgtat
     1501 caatacgaac acctgtttgt tccataatgg caagaactcg catcaaaggc atttcaatat
     1561 cgtaaaaaag tttttccaga ccattttcta aaatttcttt ttctaaaatc tgttttaatc
     1621 gaaaagtaat atctgcatct tcgcaagcat attcacaaag tttggctatt tcaatatttc
     1681 gtatatctgc ttgatttttc ccttttggtc ccactaaatc ttcataatga atagtacgat
     1741 aatgcaaata aatttcagca agataatcca tactgtggcg taattctggt tgcagaagat
     1801 aatgagcaat catggtatcg aaaagctttc ccctcagttc gatattatac atagaaagca
     1861 tcaaataatc gaactttaag ttctgtccga ttttttctat ctgttcactt tgaaaaaaag
     1921 ctttaaattc ctttaaaatg attgccgctt cttttctgtc agaaggt
//
LOCUS       DAOTKS010000322         1960 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_46788_length_1960_cov_2.903412, whole genome shotgun sequence.
ACCESSION   DAOTKS010000322 DAOTKS010000000
VERSION     DAOTKS010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46788_length_1960_cov_2.903412"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1960)
                     /locus_tag="P5236_06150"
     CDS             complement(<1..>1960)
                     /locus_tag="P5236_06150"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HRU63980.1"
                     /translation="DMLSTEEYVALLYREAAMRKQDPTVNGIASETMAFNKITEPEKA
                     GYLIMKYLDDQPTDWLDEGLRERGTMQNYQLSASGGDKNLKYFVSGNYVNENGIMLNS
                     TYDKFTFRAKLDAKLSKIATLGVNLSPTYSRQERPAVDLTDYMRFPSWLPVRHNEATA
                     ALTSKVAGDYAQPSDFNGINISGIGYDNEVWCLPGVTVWSSSNQNPVSIRERTSIFTD
                     DYKFQGNMYLTLNLLPGLQFKTSNGAYLLFREYNKKEQTSANKAGNPNSLTRQLTTHR
                     ELLSENTLNYNKKINNHELDVLLGLTMQKTNDNYNRIVGTNFPDEEILSFNLASQILL
                     DSPSIDGTTSFYYTESLVSLLGRINYAYLGKYMLSASFRADGSSKFYKGHKWGTFPAF
                     SVGWRASEEKFMKHFEWLSNLKLRFSYGLTGNNNIPQYAYMNSINTSNYVLGSGNGTL
                     VQGMVSNSTGLGNPDITWEQTSEANYGIDLGFLNSRLNFTVELYNSNTIKLLLQQPAM
                     YITGHQSYWNNIGKVNNKGIELEVNTTNIANKKFTWRTSANFATNKNTLLNYGDKEYE
                     DNFGERSEVFRAIVGQPAIQYYGYKTDGVYTTFEEVEAAKALKDENGNLFIYTKYAPM
                     VGGVKVLNLNGDNKIDTEDRTVLGDPFPD"
BASE COUNT          582 a          373 c          317 g          688 t
ORIGIN      
        1 atcagggaat ggatctccta aaacagttcg atcttccgtg tctattttat tgtcaccatt
       61 cagatttaaa acttttactc cacctaccat aggagcatat ttagtataaa taaataaatt
      121 tccattttca tcttttagtg ctttggctgc ttcaacttcc tcaaaagttg tataaacacc
      181 atcggtttta taaccataat attgaatagc aggttgtcct actatagctc gaaaaacttc
      241 acttctttct ccaaaattat cttcatattc tttatctcca taatttaaaa gggtattttt
      301 attggtagca aagtttgctg aagttctcca agtgaatttt ttattggcaa tattggttgt
      361 attgacttct agttctattc ctttattgtt aactttacct atattgttcc aatatgattg
      421 gtgaccagta atatacatag ccggttgttg aagtaacaat ttaatggtat ttgaattata
      481 aagttctacc gtaaaattca aacgactatt caaaaaacct aaatcgattc cgtaattagc
      541 ttcagaagtt tgttcccaag taatatcagg attccccaat cctgtactat ttgaaaccat
      601 tccctgaacc aatgttccat ttcctgagcc taacacataa ttactggtgt taatgctatt
      661 catataagca tattgaggaa tattattatt cccagttaac ccataactaa atctcagttt
      721 taaattagat aaccattcaa aatgcttcat aaatttttct tcagatgctc gccaacctac
      781 tgagaaagca gggaaagttc cccatttatg tcccttataa aattttgaac ttccatctgc
      841 acggaagcta gctgacaaca tatatttgcc gagataggca taattgatac gtcccaaaag
      901 cgaaactagt gactcggtat agtagaagga agttgttcca tcaattgatg gactgtccag
      961 taatatttga gaagcgagat taaaagaaag tatctcttca tcaggaaaat tagtgccgac
     1021 tattctatta tagttgtcat tcgtcttctg catggttaat cccaataaaa catctaattc
     1081 atgattattt atttttttat tgtagtttaa ggtgttttca gaaagcaatt ctctatgtgt
     1141 agttaattgt cgggtcaaag aattagggtt tcctgctttg ttagcagaag tttgttcttt
     1201 cttgttgtat tccctgaata aaagatatgc tccattggat gttttgaact gcaatcctgg
     1261 taaaagattc aaagttaaat acatattccc ctgaaatttg taatcatcag tgaaaatgct
     1321 ggttcgttct ctaatggaaa ctggattttg attacttgaa ctccaaaccg taacacctgg
     1381 taagcaccaa acttcattgt cgtatccgat cccactaatg ttaatgccat taaagtcgct
     1441 aggttgggca taatctccag caactttact ggttaatgct gctgtggctt cattgtgtct
     1501 tacgggcagc caggatggga aacgcatata atcagttaaa tctacagccg gtcgctcctg
     1561 tcggctataa gtaggtgaaa gatttactcc taaagtggca attttagata acttggcatc
     1621 taatttggct ctaaaggtga atttgtcata agtactattc aacatgatac cattttcatt
     1681 gacgtaattt cctgaaacaa aatatttcaa attcttgtct cctcctgagg cactcaattg
     1741 ataattctgc attgttcccc gctctctcaa tccctcatca agccagtcag taggttgatc
     1801 atccaaatat ttcataatca gataacctgc tttttcgggt tcagtaattt tgttaaatgc
     1861 catggtttcg gaggctatgc cattgacggt aggatcttgt tttcgcatag ctgcttctcg
     1921 atacaaaagt gctacatatt cttctgtact taacatatct
//
LOCUS       DAOTKS010000323         1955 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_46956_length_1955_cov_5.473158, whole genome shotgun sequence.
ACCESSION   DAOTKS010000323 DAOTKS010000000
VERSION     DAOTKS010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46956_length_1955_cov_5.473158"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1955)
                     /gene="carB"
                     /locus_tag="P5236_06155"
     CDS             complement(<1..>1955)
                     /gene="carB"
                     /locus_tag="P5236_06155"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRU63981.1"
                     /translation="IKLKVDLIRVPWDYDFNQLEFDALFISNGPGDPLHCSSTIEHIR
                     VAMGNNKPIFGICMGNQLLAIAGGAQRYKLKYGHRSSNQPVQLVGTQHAFITSQNHGF
                     AINNQTLDKNWAPLFLNINDGTNEGIYHTTKPWFSVQFHPEASAGPTDTEFLFDVFIK
                     TLTEKNKIIPELIEKELSLLQVKKHPYNGPEKGEIKKVLVLGSGALKIGEAGEFDYSG
                     SQALKALKEEGVQTVLINPNIATVQTSEGIADKVYFLPITPYFVERVIEKERPDGIFL
                     SFGGQTALNCGVALYQNNIFEKYKVRVLGTPVQAIIDTEDREIFNQRLSEINVKYIQS
                     QAVTHLNEAYEAAHKVGYPIIVRAAYALGGLGSGFCNNDEELKSVVEKAFSYSPQVLI
                     EKSLKGWKEIEYEVVRDRYDNCITVCSMENFDPLGIHTGESVVVAPSQTLTNSEYYKL
                     RELSIRIIRHIGIIGECNIQYALDPNSEDYRVIEVNARLSRSSALASKATGYPLAFIA
                     TKLGLGYGLAELKNSITKVTSAFFEPAVDYVVCKIPRWDLNKFNGVSHLLGSSMKSVG
                     EVMSIGRSFEEIFQKGLRMVGLGMHGFVANRDYFVDDIDDALSQPTDKRVFYLAQALH
                     EGYSIDRLYELTKIDRWFLYKLKHIID"
BASE COUNT          636 a          392 c          302 g          625 t
ORIGIN      
        1 aatcaataat atgttttaat ttgtaaagaa accatcggtc tatttttgtt aactcgtaca
       61 atcgatcaat agaataaccc tcgtgtaagg cttgtgcaag ataaaataca cgtttatcag
      121 tcggttgaga aagagcatca tcaatatcat ctacaaaata atctctattg gcaacaaatc
      181 cgtgcattcc aagtcctacc attcgcaatc ctttttgaaa gatttcttca aatgaacgcc
      241 caattgacat aacttccccc accgatttca tactgctgcc taataaatga gaaacaccat
      301 taaatttatt caaatcccaa cgtggaattt tgcagacaac ataatctacc gctggttcaa
      361 aaaaagcaga agtaactttg gtgatggaat tttttaactc tgctaaacca tatcccaaac
      421 ctaattttgt agcaataaaa gccagcggat aacccgttgc ttttgaagcc aatgctgaag
      481 aacgactcaa ccgagcattc acttcaatga cacgataatc ttcagaattc ggatcgagag
      541 catattgtat gttacattcg ccaatgatgc caatatgacg aataattcgg atggacaatt
      601 cacgtaattt gtaatattca ctattggtca aagtttgtga gggagcaact accacactct
      661 cgcctgtatg aatacccagc ggatcgaaat tttccatgct acaaactgtg atacaattat
      721 catatctatc acgaactact tcatattcaa tttctttcca gccttttaag gatttttcaa
      781 ttaaaacctg tggtgaataa gaaaaagctt tttcgacaac agattttagt tcctcatcat
      841 tattgcaaaa accacttcct aatcctccca aagcataagc ggctcgcaca ataataggat
      901 aacccacttt atgtgcggct tcgtaagctt cattcaaatg agtaactgct tgactttgaa
      961 tatattttac atttatttca gataaacgct gattgaaaat ttctctgtct tccgtatcaa
     1021 taatagcttg aactggcgtt cctaatactc gcaccttata tttttcaaaa atattatttt
     1081 ggtaaagggc gactccacaa tttaatgctg tttgtccacc aaaagacaga aagataccat
     1141 caggtcgttc tttttcaatt acacgttcta caaaataagg tgtgataggc aaaaaatata
     1201 ctttatcagc aataccttca gaagtttgta cggtagcaat attgggatta attaatactg
     1261 tttgaacgcc ttcttctttt aaggctttca atgcttgaga gccggaatag tcaaattctc
     1321 ctgcttctcc aattttcaac gctcctgagc ctaataccag tactttcttt atttcacctt
     1381 tttcaggacc attataaggg tgtttcttaa cttgtaataa acttaattct ttttcaatta
     1441 attcaggaat aattttgttt ttttcagtca atgttttaat aaaaacatca aataaaaatt
     1501 ccgtatcagt tgggcctgcc gaagcttcag gatgaaattg cacagaaaac cagggtttag
     1561 tagtatgata aataccttca ttagtaccat cattaatatt caaaaataat ggtgcccaat
     1621 ttttatcaag tgtttgattg ttaatagcaa aaccgtgatt ttgagaagta ataaaagcat
     1681 gttgtgtgcc taccagttga actggttgat tactactgcg gtgaccatat tttaatttgt
     1741 atcgttgagc tccgcctgct atagccaata gttgattccc catacaaatc ccaaaaatag
     1801 gcttgttgtt ccccatagca acacgtatat gttcaatagt agaagaacaa tgtaatggat
     1861 cgcccggccc gtttgaaatg aacaacgcat caaattctaa ctgattaaaa tcataatccc
     1921 atggtacacg aataaggtcg actttcagct taata
//
LOCUS       DAOTKS010000324         1937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_47555_length_1937_cov_5.301807, whole genome shotgun sequence.
ACCESSION   DAOTKS010000324 DAOTKS010000000
VERSION     DAOTKS010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47555_length_1937_cov_5.301807"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..329)
                     /locus_tag="P5236_06160"
     CDS             complement(108..329)
                     /locus_tag="P5236_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63982.1"
                     /translation="MSNFKMLTFFIKYGNFHLIVIYKKDIVRKCLTLRINEEHFLDLL
                     VDKEALKKECLLDGCYVIKTDLQTTDASK"
     gene            complement(365..1627)
                     /locus_tag="P5236_06165"
     CDS             complement(365..1627)
                     /locus_tag="P5236_06165"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRU63983.1"
                     /translation="MQTIDSFNFAGKKAFVRVDFNVPLDENFNITDDTRIRAAIPTLK
                     KILNDGGSVIIASHMGRPKKNPDPKYSLKHILPRVSELLGITVKFAPDCIGEETAKMV
                     AELKPGEALMLENLRFYEEEEGKPRLPETATEEEIAIAKKELKERQKKFTQTLASYAD
                     VYVNDAFGTAHRAHASTALIADYFDENHKMFGYLMEKEVAAIEKIMNNAEHPFTAIMG
                     GSKVSSKIDIIENLLNKVDNLIIGGGMTYTFIKALGGKIGDSICEDDKLELALELLEK
                     AKNKGVNMVLAVDTKIADSFSNDAHTKVVPVNEIPDGWQGLDIGPETEKIFSDIIKSS
                     KTILWNGPVGVFEFDNFAHGSLAIAQAIVEATKNGAFSLVGGGDSVACINKYGLADKV
                     SYVSTGGGALLEAIEGRVLPGIKAIRGY"
     gene            complement(1748..>1937)
                     /locus_tag="P5236_06170"
     CDS             complement(1748..>1937)
                     /locus_tag="P5236_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HRU63984.1"
                     /translation="AEVSEEDPKQYTSMKIIYEFEGEELPMEKLEKAVKLSYEKYCGV
                     MALYKKAIPVAYEIRIKN"
BASE COUNT          602 a          376 c          274 g          685 t
ORIGIN      
        1 tttttctttt cttaaataaa taagccttat ctcttccaaa cctattttta ataaagtaca
       61 aaaagcattc tctgctaaag ataaatcttt aatcgcttat aaactgtcta tttactggca
      121 tcggtagttt gtaaatctgt ctttatcaca taacaaccat ccaacaggca ttcctttttc
      181 aatgcttctt tatctaccaa aaggtctaaa aaatgttctt catttatccg cagagttaag
      241 catttgcgga caatatcttt cttataaatt actataaggt gaaaattacc atatttaata
      301 aaaaacgtca acatcttaaa atttgacatt gacgtttttt tataaaatag aatttttaat
      361 tcatttagta acctctgatg gctttaatac cgggcagtac acgaccttcg atagcttcaa
      421 gcaaagctcc accaccagtc gatacataag aaaccttatc tgccaagcca tatttattga
      481 tacaagccac tgaatcacca ccacccacca gcgaaaaagc tccattttta gtagcttcta
      541 ctatagcttg ggcaattgcc aatgaaccat gtgcgaaatt atcaaattca aaaactccta
      601 ccggaccatt ccataaaata gttttagatg atttaataat atcactgaaa attttttccg
      661 tttcaggacc aatatctaag ccttgccaac catcaggaat ttcatttaca ggaacaactt
      721 ttgtgtgagc atcattgctg aatgaatcag caattttagt atcaacagcc aaaaccatat
      781 tgacgccttt gttttttgct ttttcaagca attctaaagc caattcaagc ttatcatctt
      841 cgcaaataga atcaccaatt tttccaccca atgctttaat aaaagtataa gtcatcccgc
      901 cgccgatgat aagattgtct accttattca gtaaattttc tataatgtca atttttgaag
      961 atacttttga gccacccata atggcagtaa aaggatgttc agcattattc attatttttt
     1021 cgatggctgc tacttccttt tccataagat agccaaacat tttatggttt tcgtcaaaat
     1081 aatctgctat caatgcagta gaagcatgtg cacggtgagc tgtcccaaaa gcatcattta
     1141 cgtatacatc agcgtatgaa gccaatgttt gggtaaattt tttctggcgt tcttttagtt
     1201 ctttttttgc aattgcaatt tcttcttcag tagcggtttc aggtaagcga ggttttcctt
     1261 cttcttcttc gtaaaaacgt aaattttcta acattaaagc ttcacctggt ttaagttcag
     1321 ctaccatttt tgctgtctct tcacctatac aatcaggtgc aaattttacg gttattccca
     1381 gtaattcaga aacgcgaggt aaaatgtgtt ttaaagaata cttgggatca ggattttttt
     1441 taggcctgcc catgtgtgaa gcaatgataa cgcttcctcc atcatttaaa atttttttca
     1501 aagtgggaat tgctgcgcga atacgagtat catccgtaat gttaaaattt tcatcgagtg
     1561 gtacattaaa gtccacgcgt acaaatgcct ttttgccggc aaaattaaaa gaatctattg
     1621 tttgcataat cttcaaatat ttaaaaataa tgtttatgat tttgtagaaa aatcagcaaa
     1681 gaaccatttg caaaaatagt aaaattatgg ttcgtatttc aacctttgaa aaaactttta
     1741 tgaattctta attttttatt cttatttcat aagcgaccgg aattgctttt ttgtacaaag
     1801 ccattacccc acaatatttt tcgtatgaaa gtttaaccgc cttttcaagt ttttccatgg
     1861 gaagctcttc tccttcaaat tcgtaaatga ttttcataga agtatattgt ttgggatctt
     1921 cttcacttac ttctgct
//
LOCUS       DAOTKS010000325         1933 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_47702_length_1933_cov_5.745474, whole genome shotgun sequence.
ACCESSION   DAOTKS010000325 DAOTKS010000000
VERSION     DAOTKS010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47702_length_1933_cov_5.745474"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(230..1915)
                     /locus_tag="P5236_06175"
     CDS             complement(230..1915)
                     /locus_tag="P5236_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRU63985.1"
                     /translation="MAKFVIYEVLPRLFGNYNTTNKYNGSIEENGCGKFNSFTDKALQ
                     EIKSLGVTHIWYTGILEHATQTDYSAYGIKKDFPCIVKGKAGSPYAVKDYYDVDPDLA
                     EYVPERMKEFENLINRTHAAGLKVIIDFVPNHVARQYYSDAKPKGVEDFGERDHPEWA
                     FSPFNNFYYIPSQPFAPQFDIDGYYEFPAKATGNDQFTPSPTIYDWYDTVKLNYGVNY
                     MEGMQKQFDPIPDTWFKMLDILLFWAEKGIDGFRCDMAEMIPVEFWNWALVQVKEKYP
                     DIIFIAEIYKLPEYRNYLCNGKFDYLYDKIGLYETLRNITTRNYPARNITYAWQQLNG
                     IENYMLNFLENHDEQRIASGFFAGDGMYAQPAMIVAATLTKAPVLIYFGQELGELGMD
                     IEGFSGLDGKTSIFDYWGVKSIQAWANNGKFDGAKLTKKQKALRNFYQKLLNITLHEK
                     AITDGVMYDLEYANFNNPNFNTHEQFSYIRKYEDELLLFVLNFDDKELYTEVRIPLEA
                     FQFLELPIDQHYSGINLLDESEKISHIFLSPDVYFKTTLPPWRGKIFKLTQES"
BASE COUNT          628 a          367 c          294 g          644 t
ORIGIN      
        1 tcatgaattg tccaaaaacg gcagaattag tttcaaggat gcttgctcct ccgctcaggg
       61 aagcagtttg acgaaaatgg aatcaataat gtattgataa tcagacggga aataattaaa
      121 ttgcacttca ttatttcctg tctttcaaat cattaaatca aagtgcgaaa ctttagttat
      181 taatcaaaga tttatttaca gtttcctttc tttttctaca tctcaatgct tatgattcct
      241 gtgttaattt aaaaattttt cctctccaag gtggaagagt agttttaaaa taaacatcag
      301 gcgaaagaaa aatatggcta attttttcag attcatctaa taaatttatt ccactataat
      361 gttgatcgat tggcaattca agaaattgaa aagcctctaa tggaattctt acctccgtgt
      421 acagttcctt gtcgtcaaaa ttcaatacaa ataaaagtaa ttcatcttcg tattttcgaa
      481 tataagaaaa ctgttcatgc gtattaaagt taggattatt aaaattggca tattccagat
      541 cgtacatgac accgtctgtg atggcttttt catgaagagt aatattcaat aatttctgat
      601 aaaaatttct gagcgccttt tgtttttttg ttaattttgc tccatcaaat ttgccattat
      661 ttgcccatgc ttgtatagat ttaactcccc aatagtcaaa tatagatgtt tttccgtcaa
      721 gtccactgaa accttcaata tccattccta attcacccaa ttcttgacca aaataaatca
      781 gcaccggagc tttagttaaa gtagccgcta cgatcatagc tggttgagca tacatgccat
      841 ccccagcaaa aaatccagaa gcaattcgtt gttcatcatg attttccaaa aaattgagca
      901 tataattttc aatgccgttc aattgttgcc aggcatacgt aatgtttcgt gccggataat
      961 ttcgcgtagt tatattccga agcgtttcgt aaaggccaat tttatcatac aaataatcaa
     1021 atttaccatt acaaagataa tttctgtatt caggaagttt ataaatttca gcaataaaaa
     1081 taatatcggg atatttttct tttacctgaa cgagagccca attccagaat tctactggaa
     1141 tcatttctgc catatcacaa cgaaaaccat caattccttt ttctgcccaa aacagcaaaa
     1201 tatccagcat tttgaaccac gtatcaggta taggatcaaa ttgcttttgc atcccttcca
     1261 tataattaac tccataattc aacttaacag tatcgtacca atcataaatg gtaggcgaag
     1321 gtgtgaactg gtcgtttcct gtggctttgg caggaaattc ataataacca tcaatatcaa
     1381 attgaggcgc aaatggctga gatggaatgt aataaaaatt attgaaaggt gaaaatgccc
     1441 actctggatg atctctttct ccaaaatctt caacaccttt tggtttagca tctgaatagt
     1501 attgacgtgc cacatgattg gggacaaaat caataataac tttcaaaccc gccgcatgcg
     1561 tacgattaat caaattttca aattctttca ttctttcagg aacatattca gccaaatcag
     1621 gatctacatc ataataatcc tttacggcat aaggtgaacc tgctttccct ttcacaatac
     1681 aaggaaaatc ttttttaatc ccataagcag aataatcggt ttgggtagca tgttcaagga
     1741 tgccggtgta ccagatatgc gtaactccta acgatttgat ttcctggaga gctttgtcgg
     1801 taaaactatt aaattttcca cacccgtttt cttcaatgct gccattgtat ttattcgttg
     1861 tattatagtt tccaaacaat cttggaagta cttcataaat tacgaattta gccatagttg
     1921 aaatctaaaa tat
//
LOCUS       DAOTKS010000326         1932 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_47739_length_1932_cov_4.216835, whole genome shotgun sequence.
ACCESSION   DAOTKS010000326 DAOTKS010000000
VERSION     DAOTKS010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47739_length_1932_cov_4.216835"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            316..990
                     /locus_tag="P5236_06180"
     CDS             316..990
                     /locus_tag="P5236_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HRU63986.1"
                     /translation="MWLIDSPIGRKFIMSISGIFLVLFLLFHSCMNIVVIISPESYNQ
                     ICAFLGANWYAVIGSLVLALGFVVHILYALYLSFLNFRARGEVRYAVKATPTDVEWSS
                     KHMLILGVIIVGFIVLHLYNFWFKMQFAELSGIHTGAFDPQNGAAYVSALFSHWIYCA
                     LYIIWLVALWLHLTHGVWSALHSLGLNNKKWLPRIKVIGNIVSTIIVLMFMSVPIYYL
                     IVNILQ"
     gene            1035..>1932
                     /locus_tag="P5236_06185"
     CDS             1035..>1932
                     /locus_tag="P5236_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRU63987.1"
                     /translation="MKNNERTQEGENFILDTHIPEGPLAEKWTNYKAHQKLVNPANKR
                     KLDVIVVGTGLAGASAAASLGELGFNVLNFCIQDSPRRAHSIAAQGGINAAKNYQNDG
                     DSVFRLFYDTIKGGDFRSREANVYRLAEVSNNIIDQCVAQGVPFAREYGGLLENRSFG
                     GAQVSRTFYAKGQTGQQLLLGAYSALSRQIAKGTVKIFPRYEILDVVIIEGRARGIIA
                     RNLITGRIERYFAHAVVIASGGYGNTYFLSTNAMASNGSAVMQIYRKGAYFANPAFVQ
                     IHPTCIPLHGDYQSKLTLMSESL"
BASE COUNT          583 a          272 c          386 g          691 t
ORIGIN      
        1 ctaaaataat ttggaattaa ccatagaata cgtcatatat ggcgtctcta ctgaaaataa
       61 taaaaaagca agtagggttg gggcaaagtc acatagaaat gatttctaaa ataaaagtat
      121 taattgggtg tgatttcttt caaacaaggc gaatcaaaac aacaataaaa aaacacatta
      181 aagaaaaatt tctttaattt tttgtttctt tacgttcaaa gtagtacttt tgtggcaaat
      241 ttaagagaaa gctataatag ctttaattgt ttgattttct gaaaattaat tgcaaatcaa
      301 gtaaaaataa aatttatgtg gctaattgat tctcccatcg gaagaaaatt tatcatgagt
      361 atttcaggaa tttttctggt gcttttttta ttgttccata gttgtatgaa tattgtggtc
      421 attatttctc ctgaaagtta taatcaaata tgtgcttttt tgggagcaaa ttggtatgct
      481 gtaattggct cacttgtttt ggcattgggt tttgtcgtgc atattttgta tgctttatat
      541 ttatcttttt tgaattttcg tgctcgtgga gaagttcgtt atgctgtaaa agccacacca
      601 actgatgtgg agtggtcatc aaaacacatg ctgatattag gggtaataat agtggggttc
      661 attgttttac atttatataa tttctggttt aagatgcagt ttgctgaatt atcaggaata
      721 catacaggag cttttgatcc acagaatggt gctgcttatg taagtgcact ttttagccat
      781 tggatttatt gtgctcttta tatcatttgg ttggtggctt tgtggttgca tttaacacat
      841 ggcgtttgga gcgctttaca ttctttagga ttaaataata aaaaatggtt accccgcata
      901 aaagtaattg gcaatatagt ttctactatt attgtattaa tgtttatgtc tgttcctatt
      961 tattatttga tagtaaatat attacaataa attttttaaa aatcagagaa aagcttatca
     1021 atacaaatcc aaatatgaaa aataacgaaa gaactcagga aggtgaaaat tttattcttg
     1081 atacccatat tccagaagga cctttggctg aaaaatggac caactacaaa gctcatcaaa
     1141 aactggtgaa tcctgccaac aaacgtaagt tagatgtaat tgtagtaggg acgggtttgg
     1201 caggagcatc tgcggctgct tcgttgggtg aattaggatt taatgtgcta aatttttgta
     1261 ttcaggattc tccacgtcgt gcacattcta tagcagctca aggggggatt aatgcagcca
     1321 aaaattatca aaatgatggt gattctgtct ttcgactttt ttatgatacg ataaaaggcg
     1381 gtgattttcg ttcgcgtgaa gcgaatgttt atcgtttagc tgaagtttcc aataatatta
     1441 ttgatcaatg tgtggcacag ggtgtacctt ttgctcgtga atatggagga ttacttgaaa
     1501 atcggtcgtt tggtggtgct caggtttccc gtacttttta tgcgaaagga caaacaggac
     1561 aacaattatt gttaggtgct tattcggctt tgagtcgtca aattgccaag ggtactgtaa
     1621 aaatatttcc tcgttatgaa atattagatg tggtcattat tgaggggcgt gctcgcggaa
     1681 tcattgctcg taatttaatt acaggaagaa ttgaacgtta ttttgctcat gcagtagtaa
     1741 ttgcttccgg aggttatgga aatacctatt ttctttctac caatgcgatg gcttcaaatg
     1801 gttccgctgt gatgcagata tatagaaaag gtgcttattt tgctaatcct gcttttgtgc
     1861 aaattcatcc tacatgtatt cctttgcatg gtgattatca gtcaaaactt actttaatgt
     1921 ctgaatcgtt ac
//
LOCUS       DAOTKS010000327         1928 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_47853_length_1928_cov_4.742659, whole genome shotgun sequence.
ACCESSION   DAOTKS010000327 DAOTKS010000000
VERSION     DAOTKS010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47853_length_1928_cov_4.742659"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1928
                     /gene="dnaE"
                     /locus_tag="P5236_06190"
     CDS             <1..>1928
                     /gene="dnaE"
                     /locus_tag="P5236_06190"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRU63988.1"
                     /translation="NFIPLSTAEDKDTKEEILVTQYEGSVIEEVGLIKMDFLGLKTLS
                     IIKDTIENIKKSKGIEIDISSIPIDDPETYQLFSKGQTIGTFQFESPGMQKYLQALQP
                     TQFEDLIAMNALYRPGPMQYIPQFIKRKHGLEKIEYPFPVMEKRLKETYGITIYQEQV
                     MLLSVDLAGFTRSEADELRKAMGKKLNNKMEALKEKFIIGCQKNGYGPLEKIEQIWND
                     WIEFSNYAFNKSHATCYSLIAYQTAYLKAHYPAEFMAANLSRNKDNIDEIRKFMDECK
                     NMGINVLGPDINESDLTFSVNEKGDIRFGLGGIKGVGEGAVTNIINERNSHGKFKSVF
                     DFVERVNLSACNKRTFESLALAGAFDSLNDIQREQFFVENSKGEQVLESLIKYGNKFQ
                     IDKSMNHNTLFGLFDEIEIAKPEIPFAPQWSSLVRLNKEKELIGIYLSAHPLDEYEFE
                     INYLCNTTTSDLKNLNELTDKTLKLAGIVTSIRHATSRTGKPYGVFTLEDYNGSYEFV
                     LYGNNYIDFQKYMIKDLYLFIQATVQEKYADSNYRKKNNFTSSELELKIQKIDLLQDI
                     KNKIINSLTLMIPLQELTNDLAQQLTDVVLQNKGNINLYIQVFDDQNVANKINFFARR
                     HKIEMNSTVFQFLKEIKK"
BASE COUNT          723 a          276 c          329 g          600 t
ORIGIN      
        1 taattttatt cctttaagta cagccgaaga taaagataca aaggaagaaa ttttagtcac
       61 tcaatatgaa ggttcagtga ttgaagaagt aggattgata aaaatggact tcttgggatt
      121 gaaaactctt tccatcatca aagatacgat agaaaatata aaaaaatcaa aaggcattga
      181 gatagatatt tcttctattc ctattgatga tcctgaaaca tatcaattat tcagtaaagg
      241 acaaacaatt ggaacttttc aatttgaatc tcctggtatg caaaaatact tgcaagcctt
      301 gcaacctact cagtttgaag atttaattgc catgaatgca ttatatcggc ctggacctat
      361 gcaatatatt cctcaattca tcaaaagaaa acatggtctc gaaaaaattg aatatccttt
      421 tccagtgatg gaaaaaaggc taaaagaaac gtatggtatc accatttatc aggaacaggt
      481 aatgcttttg agtgttgatt tagcaggttt tacacgaagt gaagctgatg agcttcgtaa
      541 agctatgggc aaaaagttga acaacaaaat ggaagccttg aaagaaaaat tcattatcgg
      601 ttgtcaaaaa aatggttatg gaccattaga aaaaatagaa caaatatgga atgactggat
      661 agaattttca aactatgctt ttaacaaatc acatgctacc tgttattcgt tgattgctta
      721 tcagacagct tatttgaaag cacattatcc agcagaattt atggcagcca acctgtcaag
      781 aaataaagat aacattgatg agataagaaa attcatggat gaatgcaaaa atatgggtat
      841 caacgtactg ggtcctgata taaacgaaag tgatttgact ttttctgtaa atgaaaaagg
      901 agatattcgt tttggactgg gtggtataaa aggcgttggc gaaggtgctg tcactaatat
      961 catcaatgag cgaaattcac atggaaaatt taaaagtgtt tttgactttg tagaacgcgt
     1021 caatctttct gcttgcaata agcgaacttt tgaaagtctt gccttggcag gagcttttga
     1081 tagcttaaat gatattcagc gagaacagtt ttttgttgag aatagtaaag gcgaacaagt
     1141 tttggaaagc cttattaaat atgggaacaa atttcagata gataaatcaa tgaatcataa
     1201 tactttattt ggactttttg atgagattga aattgccaaa cccgaaattc cttttgctcc
     1261 tcaatggtcg tcgcttgtcc gtttaaataa agaaaaagaa ttaattggaa tatatctttc
     1321 tgctcatcct ctcgatgaat atgaatttga aattaattat ctttgcaata caacaaccag
     1381 cgatttaaaa aatcttaacg aactgacaga taaaacgttg aaattagcag gaattgtaac
     1441 cagtattcgt catgctactt ccagaacagg aaagccttat ggtgttttta ctttagaaga
     1501 ttataacggc tcgtatgaat ttgtacttta tggtaataac tacattgatt ttcaaaaata
     1561 tatgataaag gatttgtatc tttttattca ggctaccgtt caagaaaaat atgctgatag
     1621 caattaccgt aaaaaaaaca attttacttc gtcagaactt gaattaaaaa tacaaaaaat
     1681 tgatttatta caagatataa aaaataaaat aataaattca ctcactttaa tgattccatt
     1741 acaagaattg acaaatgatc tggctcagca actcacagat gttgttcttc aaaataaagg
     1801 aaatattaat ttatatatcc aagtatttga tgatcagaat gtcgctaata aaatcaattt
     1861 ctttgcaaga cgacataaaa ttgaaatgaa ttccacagtt tttcaatttt tgaaagaaat
     1921 taaaaaaa
//
LOCUS       DAOTKS010000328         1921 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_48115_length_1921_cov_3.415327, whole genome shotgun sequence.
ACCESSION   DAOTKS010000328 DAOTKS010000000
VERSION     DAOTKS010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48115_length_1921_cov_3.415327"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /gene="pheT"
                     /locus_tag="P5236_06195"
     CDS             complement(<1..1178)
                     /gene="pheT"
                     /locus_tag="P5236_06195"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRU63989.1"
                     /translation="MNLSYHWLQQYINIHLPVDELAEILTSIGLEVDSYEEISSVKGG
                     LEGLVIGEVLTCQPHENSDHLFVTTVNIGEVEPLQIVCGAPNVAAGQKVVVAPVGTKL
                     YAGEETFIIKRSKIRGVESYGMICAEDEIGIGTSHEGIIVLPNDAIPGTPAKEYYKIE
                     TDVVFEIDLTPNRADATSHFGVARDLAAYFSIRDKAIHLIKPSVENFKVQNQSLTIPV
                     IIENTEACKRYSAVTISGVKIKESPQWLQNYLKAIGIRPINNVVDITNFILYEIGQPL
                     HAFDADKIKGGKVIVKNLPEGTTFITLDGVERKLSAEDLMICNVEEPMCIGGVFGGLN
                     SGVTETTQNIFIESAYFNPVSIRKTARRHGLNTDASFRFERGCDPENTIYALKRCALL
                     "
     gene            1528..>1921
                     /locus_tag="P5236_06200"
     CDS             1528..>1921
                     /locus_tag="P5236_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63990.1"
                     /translation="MKQSFNLLLILLLGVQFLSSQVIKEFPIAVGSEAASALSAAFDG
                     TNYLVPIQGDDNSEYALTAQFISKAGELSGNRIDLNAYGPVAPIAIFDGTNYLLVWVD
                     AEHHLYARFLSPSGQWQGNAILIASDVIF"
BASE COUNT          637 a          354 c          288 g          642 t
ORIGIN      
        1 attaacaaag cacaacgttt cagggcataa atggtatttt caggatcaca acccctttca
       61 aaacgaaatg atgcatcggt attcaatcca tgccgacgag cagttttacg aatcgaaacg
      121 gggttaaaat aagcactttc aataaaaata ttctgcgtag tttctgtcac tccagaattt
      181 aatcctccaa aaactccacc gatacacatg ggttcttcta cattgcaaat cattaaatct
      241 tcagcactaa gcttacgttc tactccgtcg agtgttataa aagttgttcc ttcaggcaaa
      301 ttttttacta ttactttgcc cccttttatt ttgtctgcat caaaagcatg taaaggttgc
      361 cctatttcat ataaaataaa attggttata tcgactacat tatttatcgg acgaattcca
      421 atggctttta aataattttg caaccactgt ggtgattctt ttattttcac tcctgaaata
      481 gttacagccg aataacgctt gcaagcctct gtattttcaa tgattacagg aatagttaaa
      541 gactgatttt gcactttgaa attttcaacg gaaggtttta ttaaatgaat ggctttatcc
      601 ctgatagaga aataagcagc cagatcacga gctactccaa aatgagaagt agcatctgct
      661 ctatttggtg taagatctat ttcgaaaaca acgtcagttt caattttgta atactctttt
      721 gctggcgtac caggaattgc atcatttggc aagacaataa ttccttcatg actggtgcca
      781 atacctattt catcttcggc acaaatcatc ccataagatt caactccacg aattttagaa
      841 cgcttaatga taaaagtttc ttcacctgca taaagttttg ttcccactgg tgcaacaact
      901 accttttgtc ctgcagccac attgggagct ccgcaaacaa tttgcaaagg ttcaacttca
      961 ccaatgttga ctgtcgtcac aaataaatga tcagaatttt catgcggctg gcaagttaaa
     1021 acttcaccaa ttaccaatcc ttccaaacca cctttcacag aagaaatttc ttcataacta
     1081 tccacttcca aacctataga agttaatatc tctgctaatt catcaacagg aagatgaata
     1141 ttaatatact gctgtaacca atgatatgat aaattcataa tttacaattt attacaatca
     1201 acccaacagg gttttttgtt ccatttttta tagtttgcaa agataagttt tttaaaagac
     1261 atataaaatt gatttttatc ttaagcaaaa gatttttgta aaaaaataaa atttaaacat
     1321 aaaaaaagta acaaataaaa aacaacaaaa aaagatgtaa aaaaagtaca atttttaaaa
     1381 attgtactta tatttgcaaa tataatttat tgataatttt tagcttaact attttttttc
     1441 aacaaattca tattcatttt tttaaaaaat tctctgttgt attctttgta taaaaaataa
     1501 aaaattaaca ttaaaaaaat taatgccatg aaacaatcat tcaatttact tttgattcta
     1561 ttgttgggag ttcaatttct ttcctcacaa gttattaagg aattcccaat tgcagttggc
     1621 agtgaagctg cttcggcttt gtcggcagca ttcgatggca ccaattattt agttccaatt
     1681 cagggagatg acaatagtga gtacgcgctt acggctcaat ttatcagtaa agcgggagag
     1741 ttgtcaggaa atagaattga tttaaatgct tatggaccag ttgctccaat agctattttc
     1801 gatggtacca attatctcct ggtttgggtg gacgctgaac atcatcttta tgctcgtttt
     1861 ttgagccctt ctggtcaatg gcagggaaac gcaattttaa tagcttcaga cgtgattttc
     1921 g
//
LOCUS       DAOTKS010000329         1913 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_48395_length_1913_cov_5.153391, whole genome shotgun sequence.
ACCESSION   DAOTKS010000329 DAOTKS010000000
VERSION     DAOTKS010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48395_length_1913_cov_5.153391"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1588
                     /locus_tag="P5236_06205"
     CDS             <1..1588
                     /locus_tag="P5236_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HRU63991.1"
                     /translation="PLLKGETVDDLLKKLIPVIFDPTIYPKGTNQAEGVDLILTSGNN
                     YYEGVTQEEAEDFYNKMINPNDKAPISYGLNSKLVKENGIIHEKTYQINGLYGEAIKR
                     IVYWLEKAAEVAENQQQREVINTLIHYYRTGDLKIFNDYCIKWVQDTTSMVDFINGFI
                     ETYGDPLGLKGSWEALVDYKNLEATKRTEIISKNAQWFEDHSPIDKRFKKEQVKGISA
                     KVVTAAMLGGDCYPSTPIGINLPNADWIRHDYGSKSITIENITEAYDQAALKSGFNEE
                     FMWSDIERERAKKYGSLTNNLHTDLHECLGHGSGKLLHGVNPNRLKAYGSTIEEARAD
                     LFGLYFLSDPKMIELGLLPDNEAYKAEYYQYMMNGLLTQLTRIQPGKNIEEAHMRNRQ
                     LIANWVYQKGLPEKVVELKKRNGKTFVVVNNYEKLRSLFGMLLAEVQRVKSTGDYKAA
                     KNLVENYGVKVDPVLHQEILERYKKLNLAPYKGFVNPVYIPVIDKKGKITDVKISYDE
                     DFVEQNLRYSKNYSHLPTFN"
BASE COUNT          691 a          278 c          335 g          609 t
ORIGIN      
        1 acctttgcta aaaggcgaaa ccgttgatga tttgcttaaa aaattaattc ccgtcatttt
       61 tgatccaacc atttatccaa aaggcactaa tcaagctgaa ggagtagatt tgattcttac
      121 ttcaggaaat aattattatg aaggtgtaac ccaagaagaa gctgaagatt tttacaataa
      181 aatgatcaat ccaaacgata aagctcccat ttcttatgga ctaaacagca aattagtaaa
      241 agaaaatggt attattcatg aaaagactta tcagataaat ggtttatatg gcgaagcaat
      301 aaagcgtatt gtttactggc ttgaaaaagc agcagaagtt gctgaaaatc aacaacagag
      361 agaggtaatt aataccttga ttcattatta tcgaacaggt gatttaaaaa ttttcaatga
      421 ttattgtata aaatgggtgc aagataccac atcaatggtt gattttatca atggtttcat
      481 agaaacttat ggcgatccat taggattaaa aggtagttgg gaagctctgg ttgattataa
      541 gaatttggaa gcaacaaaac gtacagaaat catcagtaaa aacgcccaat ggtttgaaga
      601 tcattcacct attgacaaac gttttaaaaa agaacaggta aaaggcattt cagcaaaagt
      661 agtaacagca gcaatgttag gtggcgattg ttacccttct acacccattg gtattaatct
      721 gcctaatgcc gattggattc gtcatgatta tggttcaaaa tctattacga ttgaaaatat
      781 aacggaagct tacgaccaag ctgctttgaa aagcggtttc aatgaagaat ttatgtggag
      841 tgatatagaa cgcgaacgag caaaaaagta tggttctttg accaataatt tgcatactga
      901 tttacatgaa tgtttgggac atgggtcagg aaaacttttg catggtgtta atcccaatcg
      961 attaaaagct tatggttcaa ctatagagga agctcgagca gatttgtttg gactttattt
     1021 tctatccgat ccaaaaatga tagagctcgg cttattacct gataacgaag cctataaagc
     1081 agaatattat caatatatga tgaatggctt acttacacaa cttacgcgta ttcagccagg
     1141 aaaaaacatt gaagaagctc acatgcgtaa tcgccaatta attgccaatt gggtgtatca
     1201 gaaaggtttg cctgaaaagg tagtagaatt gaaaaagaga aatggaaaaa cttttgtagt
     1261 cgttaataat tatgaaaaat tacgttcact ttttgggatg ttattggctg aagtccaaag
     1321 agtaaaatct actggtgatt acaaggcagc aaaaaatctg gtagaaaatt atggcgttaa
     1381 agtagatcct gttttgcatc aggaaatttt ggaacgttac aagaaactca atcttgctcc
     1441 ttataaagga tttgttaatc cagtctatat tcctgttatt gacaaaaaag gaaaaataac
     1501 agatgtcaaa atttcttacg atgaagattt tgtagaacag aatttacgct actcgaaaaa
     1561 ttattctcac cttccaacat tcaattgaat ttgataataa gtaggaaaga ataaaaaatt
     1621 attgttattc ctttagaagg aattgcttga taaaattttt tgttcgacta acaaaatacc
     1681 tcgaggcttc ctcgaggtta ttcatttaaa tttttagtgt gtcattcaac aatcataatt
     1741 aagaataact ttaagtaata ttttgtacaa ttttttttaa aaaatccgtt tctgctatta
     1801 aatatattat ctaccattac aactgattaa caattttttc catctttttt gttaatgatt
     1861 ttctataata tttttttagt cattaataaa taatcagtac ctttgtgctc aaa
//
LOCUS       DAOTKS010000330         1909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_48553_length_1909_cov_2.966559, whole genome shotgun sequence.
ACCESSION   DAOTKS010000330 DAOTKS010000000
VERSION     DAOTKS010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48553_length_1909_cov_2.966559"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="P5236_06210"
     CDS             complement(<1..362)
                     /locus_tag="P5236_06210"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HRU63992.1"
                     /translation="MMFSKSLKRNVLAFLLILLVPCIKAQDEYLAEIGVIGGNGFYLG
                     DANGQPFKNPSTVYGAVFRYRFNPRTALRTEWNHGIAKGEFLDATNETVNFSNSFDAI
                     DFCGEFNFFDLMQKPNNR"
     gene            726..>1909
                     /locus_tag="P5236_06215"
     CDS             726..>1909
                     /locus_tag="P5236_06215"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047334 - diphosphate-fructose-6-phosphate
                     1-phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HRU63993.1"
                     /translation="METHKSPLQIARSSYQPKTPLILKGNVALKYGEPTESVADQKEI
                     KNLFPKTYGLPVITFEEGSEVQYPEIAVGVILSGGQAPGGHNVIAGLFDGLKKLNSKN
                     KLYGFLDGPSGLVDHKYVELTSEIIDEYRNTGGFDIIGSGRTKLEQEWQFEQGAEICT
                     KLKIKAIVIIGGDDSNTNACVLAEYYAQKNYPIQVIGCPKTIDGDLKNEMIETSFGFD
                     TACKVYSELIGNIQRDASSAKKYWHFIRLMGRSASHITLECALQSQPNITLISEEVEG
                     KNLSLNDIVEYIVKIIVNRANYGVNFGTVLIPEGLIEFIPGMKKLIQELNDLLANNDD
                     FVALNTDDEKRQYIKGMLSHETSQLYRSLPKGIARQLAMDRDPHGNVQVSLIETEKLL
                     IE"
BASE COUNT          651 a          312 c          372 g          574 t
ORIGIN      
        1 ttgcgattat tcggcttttg catcaaatca aagaaattaa attctccaca aaaatcaatt
       61 gcgtcaaatg aattgctgaa atttaccgtt tcatttgttg cgtcgagaaa ttctcctttt
      121 gcaatgccat gattccattc ggtgcgtaag gcagtacgag gattgaaacg ataacgaaaa
      181 actgcgccat aaacggttga aggattttta aaaggctgtc cgttggcatc gcccaaataa
      241 aaaccatttc caccaattac tccaatttct gccaaatatt cgtcctgagc tttgatgcag
      301 ggaacaagca aaataagcaa aaatgccaac acgttacgtt ttaatgactt gctaaacatc
      361 atattgaaat gctatgaata agtgcgttat acaataaaat tataacgtgt tttttcctca
      421 taaatttttc gattcaacac aaatgcagcc acaaaaataa tgataatctt tcaatttagt
      481 tgaattggct gaagggaggc caatttattt aacaaaaaat tgcaaatagg cacactatta
      541 aatgcttacg aattttcaag taaaattatc gaaatagcag atagttttga aaaattttct
      601 cgagattgaa attacgaagt tgagaaaaca ttttacttgt attattcaat tttatcatta
      661 attttgcaaa taaattatca agaaaactta catttgaata tcaaatcatt aataaaattt
      721 ttaatatgga aacacataaa agtcctttgc agatagctcg ttccagctac cagcctaaaa
      781 cacctttgat attgaaagga aatgtggctt taaagtacgg cgaacctacc gaatcagttg
      841 ctgatcagaa agaaataaaa aacttattcc ctaaaactta cggtttaccg gtcattactt
      901 ttgaggaagg atcggaagtt cagtatccgg aaatagctgt tggagttatt ttgtcaggcg
      961 gacaggcacc tggcggacac aatgtgattg cagggctttt tgacggattg aaaaaattaa
     1021 attcaaaaaa taaactttac ggttttttgg atggacctag tggattggtt gatcacaaat
     1081 atgttgagct gacctctgaa ataattgatg aataccgcaa tacaggcggt tttgatatta
     1141 ttggttccgg aagaaccaaa ttggaacagg aatggcagtt tgagcaaggt gccgaaattt
     1201 gtacaaaatt gaagattaag gcaattgtca ttattggtgg cgacgattcc aataccaatg
     1261 cttgcgtatt ggctgagtat tatgcacaga aaaattatcc cattcaggta attggatgtc
     1321 ccaaaacaat agatggcgat ttgaaaaacg aaatgattga aacttccttt ggttttgata
     1381 cggcatgcaa agtttattcg gaacttattg gaaacattca acgagacgcc agttctgcaa
     1441 aaaaatactg gcattttatt cgtttgatgg gacgttcggc ttcgcatatt acattggaat
     1501 gcgcactcca aagccagccc aacataacgc taatttctga agaagttgaa ggtaaaaatt
     1561 tgagtttgaa tgatatagtt gaatatattg ttaaaataat tgtcaatcgt gccaattatg
     1621 gagtaaattt tggtactgtg ctgattcccg aaggtttgat tgaatttatt ccgggcatga
     1681 aaaaactgat tcaggaactg aatgatttgt tggccaacaa tgatgatttt gtggcattga
     1741 ataccgatga tgaaaaacgt cagtatatca aaggcatgtt gagccatgaa accagccagc
     1801 tgtatcgtag cctgccaaag ggaattgccc gacaattggc aatggatcgt gatccgcatg
     1861 gcaatgtgca ggtttcactt atcgaaactg aaaaattgtt gatagaaat
//
LOCUS       DAOTKS010000331         1907 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_48597_length_1907_cov_4.701944, whole genome shotgun sequence.
ACCESSION   DAOTKS010000331 DAOTKS010000000
VERSION     DAOTKS010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48597_length_1907_cov_4.701944"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /gene="tyrS"
                     /locus_tag="P5236_06220"
     CDS             complement(<1..1078)
                     /gene="tyrS"
                     /locus_tag="P5236_06220"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRU63994.1"
                     /translation="MNFVEELRWRGMIQDIMPGTEEYLLKEKTAGYVGIDPTADSLHI
                     GHLVGVMMLKHLQRAGHTPIALIGGATGMIGDPSMKLAERKLLDEETLRHNQEAIKAQ
                     LTKFLDFESDIPNKAIMVNNYNWMKDYTFLNFIRDIGKHITVNYMMSKDSVKKRLSAD
                     STEGMSFTEFSYQLIQGYDFLYLYRKKGCRLQMGGSDQWGNITTGAELIRRIEGGEAY
                     AITCPLITKADGGKFGKTENGNVWLDRHYTSPYQFYQFWINVSDEDAAKYIKIFTTLS
                     KEEIDALIEEQQKAPHLRPLQKKLAEEITIMVHSRQDYEMAVNASQILFGKATSETLR
                     SIDEETFLQVFDGVPQFHISKEALS"
     gene            complement(1258..1330)
                     /locus_tag="P5236_06225"
     tRNA            complement(1258..1330)
                     /locus_tag="P5236_06225"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1295..1297),aa:Phe,seq:gaa)
     gene            complement(1448..>1907)
                     /locus_tag="P5236_06230"
     CDS             complement(1448..>1907)
                     /locus_tag="P5236_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU63995.1"
                     /translation="VGINPYKLEVDNEGDVYVVSRGNNLDIKPKWYRIDSKSNEVVQI
                     FNDLPVVNFVIHNDTAYLYNFDFVTNDYWIKSFNCKTEQIIRDHFITDDTVIERPYCI
                     YAHPVNGNVYITDAKNYTSNSDLFCFDKNGKLRYSIKGIGLNPNAVLSVP"
BASE COUNT          607 a          349 c          321 g          630 t
ORIGIN      
        1 gagaaagtgc ttcttttgaa atatgaaatt gaggaacgcc atcaaaaacc tgaagaaaag
       61 tttcttcgtc aatggaacgc aaagtttcag atgtcgcttt accaaaaagg atttgggaag
      121 cattcactgc catttcataa tcttgacgag agtgaaccat gatggtaatt tcttctgcca
      181 gtttcttttg caacggacgc aggtgtggtg ctttctgttg ttcttcaatc aaagcatcaa
      241 tttcttcttt tgaaagagta gtaaaaattt taatatattt agccgcatct tcgtcactta
      301 cgtttatcca aaattggtaa aactggtaag gtgaagtgta atgacggtcg agccatacat
      361 taccattttc cgttttgcca aatttgccac cgtcggcttt ggtaatcaac gggcaagtaa
      421 tggcataggc ttctcctcct tcgatacgac gaataagttc tgctccggtg gtaatgttgc
      481 cccattggtc ggagccgccc atttgcaagc ggcagccttt tttacgatac aaatacagaa
      541 aatcgtatcc ctgaatcaac tgataagaaa attctgtaaa agacatacct tcagtggaat
      601 cggcactgag tcgcttcttt accgaatctt tagacatcat ataattaaca gtaatatgtt
      661 tgccgatgtc gcggataaaa ttcagaaaag tataatcttt catccaatta tagttattga
      721 ccatgatagc tttattgggt atatcacttt cgaagtcgag aaattttgtc aattgtgctt
      781 ttattgcttc ttgattgtgg cggagagttt cttcatccag taattttcgt tcagccaatt
      841 tcatggatgg atcgcctatc attcctgtag caccacctat caaggcaata ggtgtatgtc
      901 ccgctcgttg cagatgtttt agcatcataa cacccaccag atgaccgata tgaagagaat
      961 cggctgtagg gtcaatacct acatagccag cagttttttc ttttaataaa tattcttctg
     1021 ttccaggcat aatgtcctga atcataccac gccaacgaag ttcttcaaca aaattcataa
     1081 acaaataaaa gttggatttt taaaatggat acaaagttaa aactttattt tcggataaca
     1141 aacctcataa agagcaatta ttatcgataa ttacataaga agctgaaaga agagaaagat
     1201 tattttctta ataaaaaaac ctgttgcact ttatgataac aggtttaaat ttatgagtgg
     1261 taccacctgg aatcgaacca gggacacaag gattttcagt cctttgctct accaactgag
     1321 ctatgatacc atttattttg agactgcaaa tgtatttttt ttttttgaaa tacacaagtg
     1381 tcattctttt atcaaataaa agaaatgact ttaaattaat aatctattga aaactaattc
     1441 cttaaattta aggaacggac aatacagcat ttggatttag cccaattccc ttaatactgt
     1501 atcttaattt tccattttta tcaaaacaaa ataaatcact attcgaagtg taatttttag
     1561 catcggtgat gtaaacattt ccattgacag gatgagcata aatgcaatag ggacgttcaa
     1621 ttacggtatc atcggttatg aaatgatctc tgatgatttg ttctgtttta cagttaaaac
     1681 ttttaatcca ataatcattg gttacaaaat caaaattata taaataagct gtatcattgt
     1741 gaatgacaaa atttacaact ggaagatcat taaaaatctg aactacttcg ttgcttttgc
     1801 tgtcaattcg gtaccatttt ggtttaatat ccaaattgtt gcctcttgat accacataca
     1861 catcgccttc gttatctact tccaatttat aaggatttat cccaaca
//
LOCUS       DAOTKS010000332         1893 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_49087_length_1893_cov_4.112078, whole genome shotgun sequence.
ACCESSION   DAOTKS010000332 DAOTKS010000000
VERSION     DAOTKS010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49087_length_1893_cov_4.112078"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="P5236_06235"
     CDS             complement(<1..772)
                     /locus_tag="P5236_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRU63996.1"
                     /translation="MSSKKLQASDTSGKFMFSLWIAKRYLFSKKSHNAINVISAISAI
                     GVAVGTMALVCVLSVFNGFESVISDMFSAFDPDLKITVTQGKTFDITTPAWQEVKKMK
                     AIDCFTEVIEENALLRFKDKQMPAVIKGVSDDFERMTKIDSIMYDGKFILFDGGFQRA
                     VIGIGIANILGLGAHFIDPLYIYAPKRTSDINLLRPETSFNQVGTFVSGIFAVNQLEY
                     DDQYVIVSLALARDLFEYDSTKVSAIELKLTNLSHQKEI"
     gene            complement(820..1173)
                     /gene="rbfA"
                     /locus_tag="P5236_06240"
     CDS             complement(820..1173)
                     /gene="rbfA"
                     /locus_tag="P5236_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRU63997.1"
                     /translation="MQVTRQQRIEKLIQKELGEIFLLYAREIPGTIIGVTNVRISPDL
                     SIARIHISIYPTNKSGEIFENINHENKSVRFELGKRVRNQLRIIPELHFYLDDSLDYA
                     EHIDQLLKGNIPPKQ"
     gene            complement(1270..>1893)
                     /locus_tag="P5236_06245"
     CDS             complement(1270..>1893)
                     /locus_tag="P5236_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRU63998.1"
                     /translation="KMGKSLGNFITLEEFFTGNHPLLTQAYSPMTIRFFMLQAHYRST
                     VDFSNEALQASEKGLQRLLEGYERLQKIQPSEKSTVNLSDLRQNCEAAMCDDLNTSVV
                     ISHLFEAVKTIYLINDEKETISSDDLKEVKDVFHLFIEEILGLKTENADSSKLEAYKK
                     AVDLLLNIRLEAKKNKNWELSDKIRSELTALGFEIRDTKDGFEWKLN"
BASE COUNT          593 a          347 c          283 g          670 t
ORIGIN      
        1 gtatttcctt ttggtgggaa agatttgtta gtttcaattc aattgccgaa actttagtag
       61 aatcatattc aaataagtca cgagccaatg caagagaaac aatcacgtat tgatcgtcat
      121 attccaactg attgactgca aaaattcctg atacaaaagt tcctacctga ttgaagctgg
      181 tttcgggtcg aagcaaattt atatcagaag ttctttttgg tgcgtaaata tacaatggat
      241 caataaaatg agctcctaaa ccaagaatat tggctattcc aattccaata actgctcttt
      301 gaaaacctcc atcaaacaaa ataaatttac catcatacat gatgctatca atctttgtca
      361 tcctttcaaa atcgtcactt acgcctttga taacagcagg catctgttta tctttgaaac
      421 gtaataaagc attttcttca attacttctg tgaagcaatc aatagctttc atctttttta
      481 cttcctgcca tgctggcgta gttatatcaa aagtttttcc ttgcgtgacg gtaattttca
      541 aatcaggatc aaaagccgag aacatgtcgg aaataacaga ttcaaaaccg ttgaaaactg
      601 acaatacgca taccagagcc attgttccaa ctgctacacc tattgctgaa atggctgaaa
      661 tgacattaat tgcattgtgt gattttttag aaaataaata tcgtttggca atccacaaag
      721 aaaacataaa ttttccagaa gtatcacttg cttgcaattt ttttgaactc atgttttcag
      781 cttttttcga aaaacttttc agggaaaatt tactgagatt tattgttttg gtggaatatt
      841 ccctttcagt aattgatcaa tatgttccgc ataatccagt gaatcgtcca gataaaaatg
      901 aagttcagga ataatccgta attgatttct gactcttttt cccaattcaa agcgtacaga
      961 tttattttca tggttgatgt tttcaaaaat ttcaccgctt ttattagttg gataaatact
     1021 aatatgaata cgagcaatac ttaaatctgg actaattcga acattagtaa cgcctataat
     1081 agttcctgga atttctctgg catataacaa gaaaatttcc cccaattcct tttgtatcag
     1141 tttttctatt cgctgttgtc gggtaacttg cattttttta atttttaatt tgcctttcag
     1201 cgtcatttta aaaatgaaac gtaatttttt tctctttttg agaagatatc cttctctctt
     1261 aataacaatt taatttaatt tccattcaaa accatctttg gtgtctctga tttcaaaccc
     1321 caaagctgtt aattcactac gaattttgtc gctcaattcc cagttcttgt tctttttagc
     1381 ttccagtcgg atatttaaaa gtaaatccac cgctttttta taagcttcca gttttgatga
     1441 atcggcattt tctgttttta aaccaagtat ttcttcaata aaaagatgaa aaacatcttt
     1501 tacttctttc aaatcgtccg aagaaattgt ttctttttca tcattaatga ggtaaatggt
     1561 tttcactgct tcaaacaaat gagaaattac caccgatgta ttcaaatcgt cgcacatggc
     1621 agcttcacaa ttttgacgca aatcagaaag atttactgtt gacttttcgg aaggttggat
     1681 tttttgcaaa cgttcataac cttcaagtaa acgctgtaaa cctttttctg aagcttgtaa
     1741 agcttcatta ctgaaatcaa ctgtgctacg gtagtgagct tgtagcatga aaaaacgaat
     1801 agtcattgga ctgtaagctt gtgtcaacaa aggatgattg ccagtaaaaa attcttcgag
     1861 tgtaataaaa tttcccaacg attttcccat ttt
//
LOCUS       DAOTKS010000333         1864 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_50154_length_1864_cov_4.329464, whole genome shotgun sequence.
ACCESSION   DAOTKS010000333 DAOTKS010000000
VERSION     DAOTKS010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50154_length_1864_cov_4.329464"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..715)
                     /locus_tag="P5236_06250"
     CDS             complement(191..715)
                     /locus_tag="P5236_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRU63999.1"
                     /translation="MRAVEPEDLEKLYKWENNPQLWLVGNARNPYSHFTLKQYILNSD
                     KDIYENKQLRLMIVLKENNETIGTIDLFDFDIHNSRISLGIYVENKFQRKGYATDSLR
                     LVQEYVFDYLKINQIYAFVAEKNTPSIHIFEQEKFEKNGILKNWIKTLDDFQNIFVFQ
                     KFRENYLLQKNKIT"
     gene            complement(744..1772)
                     /gene="recA"
                     /locus_tag="P5236_06255"
     CDS             complement(744..1772)
                     /gene="recA"
                     /locus_tag="P5236_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRU64000.1"
                     /translation="MAEKTADEIKTTSPNAEKLKALQLAMDKIEKDYGKGTIMRMGDN
                     KVVDIDVIPTGSLGLDIALGVGGYPRGRIIEIYGPESSGKTTLAIHAIAEAQKAGGIA
                     AFIDAEHAFDRAYAENLGVNTDELLISQPDNGEQALEIADQLIRSSAVDIIVIDSVAA
                     LTPKAELEGDMGDAKLGLQARLMSQALRKLTANISKTKTTCIFINQLREKIGVMFGNP
                     ETTTGGNALKFYSSVRLDIRRVTPIKDGEEVIGYQTRVKVVKNKVAPPFRKAEFDIMF
                     GEGISKAGEILDLGVDYGIIKKSGSWFSYNDTKLAQGRDAAKTVIKDNPELAAELEAK
                     IKEAIKQK"
BASE COUNT          559 a          348 c          280 g          677 t
ORIGIN      
        1 gaaattttat tattgaatat gaaaatagcc aaacatcagt tgaaaaagga gaaacagttt
       61 tgatccctgc ttcaatagat gaagttaatt taattcctgg tgaaggaact gtaaaattac
      121 tggaagtgta tatagactga taaaaagcag taaaattttt ttaagcttct ctgttttttt
      181 aatcaattag ttaagtgatt ttattcttct gtaataaata attttcccgg aacttttgga
      241 aaacaaaaat attttgaaaa tcatctaaag tttttatcca atttttcaaa ataccatttt
      301 tttcaaattt ttcctgttca aaaatgtgaa tgctgggtgt atttttttct gctacaaaag
      361 catatatttg attgattttt aaataatcga aaacatattc ttgaaccaag cgaagactat
      421 cagtagcata tccctttctt tgaaatttat tttccacata tattcccaga gaaatacgac
      481 tattatgaat atcaaaatcg aataagtcaa tggttccgat ggtttcatta ttttctttta
      541 atacaatcat caaacgcaat tgcttgtttt cataaatatc tttatcggaa tttaaaatat
      601 attgtttcaa cgtaaagtga gaataaggat ttctcgcatt tcccaccaac cacagttgag
      661 gattgttttc ccatttataa agtttttcca gatcctcagg ttctactgcc ctcaaacgta
      721 tactctcgtt ttctaataac aatttatttt tgctttatcg cttccttgat ttttgcttcc
      781 aattctgctg ccaattcggg attatcttta atcaccgttt ttgcagcatc tcttccttgt
      841 gctaatttgg tgtcattgta actaaaccaa gaaccacttt ttttaatgat accatagtca
      901 acgcctaaat ccaaaatttc accggcttta gaaatacctt ctccaaacat aatgtcgaat
      961 tcagcctttc ggaaaggagg agccacttta ttttttacca cttttacacg agtttgatat
     1021 ccaattactt cttcaccatc ttttatagga gttactctac gtatatctaa tcggacagaa
     1081 gaatagaatt ttaaagcatt tccccccgta gttgtttctg gattaccaaa catgacgcca
     1141 attttttcac gcaactgatt gataaaaata cacgtagttt tagtcttgct aatgttggct
     1201 gtcaattttc gcaaagcttg cgacatcaag cgagcttgaa gtcctaattt agcatcaccc
     1261 atatcgcctt ccagttcagc ttttggtgta agagctgcta ctgaatcgat cacaataata
     1321 tctaccgctg aagaccgaat cagttggtcg gcaatttcga gggcttgttc cccattatcg
     1381 ggttgcgaaa ttaaaagttc atcggtattt acgcctaaat tttctgcata agccctatca
     1441 aaagcatgtt cagcatcaat aaaagcagca atgccacccg ctttttgagc ttcggcaata
     1501 gcgtgaatgg caagtgtggt ttttccagaa gattctggtc cgtatatttc aataatcctt
     1561 ccacgtggat aacctcctac accaagggca atgtctaaac caagtgaacc agttgggatc
     1621 acatcaatat caactacttt attgtcaccc attcgcataa tggttccttt cccatagtct
     1681 ttttcaattt tgtccattgc cagttgcaaa gcttttaatt tttcggcgtt aggggaagtt
     1741 gtttttattt cgtctgctgt tttttctgcc atgataattc agttttttat ttttaattga
     1801 tgattgagga aaattttaga aaattattgt tttacaaaga taaaaaattt ttatttcaac
     1861 actt
//
LOCUS       DAOTKS010000334         1862 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_50230_length_1862_cov_5.091312, whole genome shotgun sequence.
ACCESSION   DAOTKS010000334 DAOTKS010000000
VERSION     DAOTKS010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50230_length_1862_cov_5.091312"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1862
                     /locus_tag="P5236_06260"
     CDS             <1..>1862
                     /locus_tag="P5236_06260"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRU64001.1"
                     /translation="EQYQQEVEEELDFNNFLEDLLFVAENPIDLNRATREELEKLPFL
                     SDIQIENILYYLYQFGPMQTIYELQLIDGLDMTDIRRMLPFVVVHPLKKENPKLYWHD
                     LLNYGKNDLYIRLDRKLETKRGYLSISPEKNSDVSSKPYLGDPFYNSIKYRYHFRDKI
                     YFGFTTEKDPGEPFLDKIQTNYDFLSGYLQLNNIGKFKTIILGDYRANFGQGLVLRQE
                     FSMGKSSYVMNVIPRGSGLKKYSSTDEYNFFRGLGTTLQLGNTEISAFYSNKQIDGDT
                     IQGCFSSIYKTGYHRTSNEFKKKKTVNQQVVGGNLTYYFPHLQIGATVVHTLFNHFLE
                     KEETTYNHFYFSGNQQTTGGINYRFRWKKLNFFGETALSNHFSWATLNGCFFSPFSLL
                     DIVVLYRNYSPDYDTFYANAFSESTRINNEKGMYLGAELRPIKNWKFSAYADSYRFSW
                     VKYRVDAPSFGNDYLLQVDFSPSRMVNMFWRLRYEEKQENLSQDTSIMSFVVPLRKAS
                     LRYQLNYVAGNFSFRTVLEGNLAQKSEEASWTYGLTAYQDLSYEFRSFPLRLDFRYQF
                     FDATSYENRVYAYEKDVLYAFSIPMNYGLGSRYYLNLRYKFNHQLSCWLKFAQTV"
BASE COUNT          599 a          273 c          336 g          654 t
ORIGIN      
        1 gaacaatatc agcaggaagt agaagaagaa ttggatttca acaatttttt agaagattta
       61 ttattcgtgg ctgaaaatcc aatcgattta aatcgtgcta cacgagaaga attggaaaaa
      121 ttaccttttc tatcggacat tcaaattgaa aatattcttt attatctgta tcagtttggt
      181 cctatgcaaa ccatttatga actacaactc attgacggtc tggatatgac agacattaga
      241 cgaatgttgc cttttgtagt agttcatcca ctcaagaagg aaaatccgaa attatactgg
      301 cacgatcttt tgaattatgg aaaaaatgat ttatacatcc gtctggatag aaagctcgag
      361 accaaaagag gatacctatc aatttcacca gaaaaaaatt ccgatgttag ttcaaagcca
      421 tatttaggag atccatttta taattcaata aaatatcgtt atcattttcg ggataaaatt
      481 tattttggtt ttaccactga aaaagaccca ggagaaccat ttttagataa aattcaaaca
      541 aattatgatt ttctttcagg ttatttacaa ttgaataata ttggcaaatt taaaaccatt
      601 attttgggcg attatagagc caattttggt caaggtcttg ttttgcgtca agagtttagt
      661 atggggaaat catcttacgt tatgaatgta attccacgtg gatcaggatt aaaaaaatat
      721 agttctactg atgaatataa tttttttcgc ggattgggca caactttaca attggggaac
      781 acagaaattt cggcatttta ttccaataaa caaatagatg gcgacaccat tcaaggttgt
      841 ttttcaagta tttacaaaac aggttatcat agaacaagta atgaattcaa gaagaaaaaa
      901 acggttaatc aacaggttgt aggagggaat ttgacttatt attttccgca tttacaaatt
      961 ggtgcaacag tagttcatac tttgttcaat cattttttag aaaaagaaga gaccacctat
     1021 aatcattttt atttttctgg gaatcaacaa actacgggcg gaataaatta ccgtttccgg
     1081 tggaaaaaat taaatttttt tggagaaact gctcttagca atcatttttc atgggcaact
     1141 ttaaatggct gtttcttttc tcctttttct ttgctggata tagtggtttt atatcgcaat
     1201 tattctcctg attatgacac gttttatgcc aatgcttttt cggaatctac gcgaatcaat
     1261 aatgagaaag gaatgtattt gggagccgaa ttacgtccga taaaaaattg gaaattttct
     1321 gcctatgcag atagttatcg tttctcgtgg gttaaatatc gggttgatgc accttcgttt
     1381 ggaaatgatt atttgcttca ggttgatttt agtccttctc gcatggttaa catgttttgg
     1441 agattgagat atgaagaaaa acaagaaaat ttatctcaag atacttcaat aatgtctttt
     1501 gttgtacctt taaggaaagc ctccttacgt tatcagctaa attatgttgc tggaaatttt
     1561 agctttcgta ctgttctgga aggcaatctt gctcaaaaaa gtgaagaagc aagttggact
     1621 tatggtttaa cggcttatca ggatttgagt tatgaatttc gttcttttcc tttaagactt
     1681 gattttaggt atcagttttt tgatgcaact tcttacgaaa atcgtgttta tgcttatgaa
     1741 aaagatgtgt tgtatgcttt ttccattcct atgaattatg gtttaggaag cagatactat
     1801 ttgaacttaa ggtataagtt caatcatcaa ctttcttgct ggttaaagtt tgctcaaact
     1861 gt
//
LOCUS       DAOTKS010000335         1862 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_50236_length_1862_cov_3.377975, whole genome shotgun sequence.
ACCESSION   DAOTKS010000335 DAOTKS010000000
VERSION     DAOTKS010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50236_length_1862_cov_3.377975"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="P5236_06265"
     CDS             complement(<1..193)
                     /locus_tag="P5236_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HRU64002.1"
                     /translation="MLKEIVSISGKPGLYKMISQGKNIVVVESLIDGKRIPAFTKDKI
                     VSLKDISIFTDSKEISLGEV"
     gene            complement(294..1013)
                     /gene="hisA"
                     /locus_tag="P5236_06270"
     CDS             complement(294..1013)
                     /gene="hisA"
                     /locus_tag="P5236_06270"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HRU64003.1"
                     /translation="MIEIIPAIDIIGGKCVRLSQGDYEQVKIYDENPIEVAKRFVEAG
                     FRRLHLVDLEGAKAQHIVNYRVLEKIKSSTPLIVDFGGGLKTNEDIRIAFECGADMIT
                     VGSVAVKNPETFVQWISQYGAEKIILSADVRNNKIAVSGWTENTEIELIPFIHQYFQK
                     GIQKVLCTDIGKDGMLEGTAIELYKKIMKEISSLYLIASGGVSSIHDIENLEEIKVPA
                     VVVGKALYEGKIKLNEFYKYL"
     gene            complement(1152..1736)
                     /locus_tag="P5236_06275"
     CDS             complement(1152..1736)
                     /locus_tag="P5236_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU64004.1"
                     /translation="MIHNFSQSKLYENEQIKKADKFYHDVLRVSDIKRFNYQLSEERK
                     MQLHDIDLLLTYQNKSYKVSEKFRSKDFGDLYLEIYSKYPKVSGWLNEGISDYIMYFT
                     PEKVYCIDDLSLKKFYKENLISLLPSFWFENLKTSDRNIISKKIYVNNIPYIISIIKA
                     LNETSEGDNWITMGITIPFNMLKINGVKFLKFSF"
BASE COUNT          664 a          287 c          225 g          686 t
ORIGIN      
        1 gaacttcacc caaagaaatt tcctttgaat cggtaaaaat tgaaatatct ttcaatgaca
       61 caattttatc ttttgtaaaa gcaggaattc gttttccatc gattaaagat tctaccacta
      121 caatattttt cccttgcgaa atcattttgt acaaaccagg tttacctgaa atggagacaa
      181 tttcttttaa catattctgt aaaaattaaa aagtaatttg agttaattgg aaagattttt
      241 taaatacttt tttataaaga ataaatggca aattaatatg ataaagcaat aatttataaa
      301 tatttataaa attcattaag ttttattttc ccttcatata gagctttccc caccacaaca
      361 gcaggaactt taatttcttc taagttttca atatcatgaa tagaacttac acctccgctt
      421 gcaattaaat acaaagaaga tatttctttc ataatttttt tataaagctc aatggcagtt
      481 ccttccagca tgccatcttt gcctatgtcg gtgcataaaa ctttttgaat tcctttttga
      541 aaatactggt gaataaaagg tattaattca atttcagtat tttctgtcca gcccgataca
      601 gctattttat tgtttcttac gtcagcactt aaaattattt tttctgctcc gtattgtgaa
      661 atccattgaa caaatgtttc aggattttta actgccacac taccaactgt tatcatatca
      721 gcaccacatt caaaagcaat acggatatct tcatttgttt taagcccacc gccaaaatcg
      781 acaattaatg gtgtagagga ttttattttt tctaaaactc gataattcac aatatgttga
      841 gctttagctc cttccaaatc tactaaatgc aaacgtcgaa aacctgcctc aacaaatcgt
      901 tttgctacct caattggatt ttcgtcataa attttaactt gttcataatc accttgagat
      961 aaacggacac atttaccacc aataatatca atagctggaa taatttctat cacaatactt
     1021 tattgaaatt acatttaatg agaagcaaaa ttataaaaat caaaaagaag aagcaaatga
     1081 agagaaaaga aatcaatagt tgaataattt ttattgtaaa tataagccaa aacaaaaaag
     1141 agaaaaaatc actagaatga aaattttaaa aattttactc cattgatttt aagcatatta
     1201 aagggaattg taatacccat tgttatccaa ttgtcccctt ctgaagtttc attaagggct
     1261 ttgattattg aaataatgta tggaatatta ttaacgtaaa ttttcttaga aataatgttt
     1321 ctatctgatg ttttcaaatt ttcaaaccaa aaagaaggta acaaggaaat caaattttct
     1381 ttgtaaaatt tttttagtga gagatcgtca atgcaataaa ctttttcagg agtaaaatac
     1441 atgatgtaat ctgaaattcc ttcatttagc caacctgaaa cttttggata tttactataa
     1501 atttcaaggt acaaatcgcc aaaatcttta gatctgaatt tttcagaaac tttataagat
     1561 ttattttgat atgtcagcag caagtcaata tcgtgaagtt gcatttttct ttcttctgat
     1621 aattgataat taaaacgttt aatatcagac acacgtaaaa catcatgata aaatttatcc
     1681 gcttttttta tttgttcatt ttcataaagt ttactttgag agaaattgtg aatcattttt
     1741 atctatggtt tattatattt ttatattata tttttatatt atatttttat attatatttt
     1801 tatttcactt aataaagaaa ttccttaaaa ttgactatcc tcgccgcaag cagcgaggta
     1861 tt
//
LOCUS       DAOTKS010000336         1855 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_50499_length_1855_cov_3.181111, whole genome shotgun sequence.
ACCESSION   DAOTKS010000336 DAOTKS010000000
VERSION     DAOTKS010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50499_length_1855_cov_3.181111"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..668
                     /locus_tag="P5236_06280"
     CDS             75..668
                     /locus_tag="P5236_06280"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HRU64005.1"
                     /translation="MIYSEQTHPNEKIRGSIEVICGSMFSGKTEELIRRLRRAQFARQ
                     KVEIFKPRIDTRYSVDEVVSHDRNTIPSTTVDSSASILLLSSNVDVVGIDEAQFFDDG
                     LVDVCNQLANDGIRVIVAGLDMDFRGVPFGPMPALLAIADDVYKVHAICVRCGALAYI
                     SHRLVENDKQVLLGELDQYEPLCRKCYNEVQKKSSRH"
     gene            684..1841
                     /locus_tag="P5236_06285"
     CDS             684..1841
                     /locus_tag="P5236_06285"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRU64006.1"
                     /translation="MSENSKPYTFDRVVRIIITLTIIFLLFLLLKKISKVLLPFLVGW
                     LLAYLLQPIVHFFQHKLKFKSRLLSVIVTLLLFIGFFVGLGFILVPIIIDETRTVVGL
                     INSYIQSTDIDAILPVAWQNQLKEFLAKFDFQTLFQNENFLSILKKFLPEVWNFVSGA
                     LNFILSWAVIIIIFLYLFFILMDYERISTKWIELVPQKYRSIVWQIAQDIADGMNRYF
                     RGQALVAMCVGILHMIGLSIIGLPMSILMGLTIGILNLVPYLELLGIIPCFFLAVLKS
                     AETGQNFWLVVLSIAIVWLVVQAIQDLILVPKIMGKVTGLHPALILLSLSIWGALLGL
                     AGMIIALPITSLILSYYRRFIVLDERRTTHSGTFNEEWLSKRKDKDDSSSS"
BASE COUNT          519 a          275 c          383 g          678 t
ORIGIN      
        1 ggtgtaaagt tacaacaatt gagagagaaa taaaaaagaa attttaattt tcaacaaaac
       61 ctgtttttaa aaagatgatt tattcagaac agacccatcc caatgaaaaa atacgtggca
      121 gtattgaagt tatatgtggt tcgatgtttt ctggtaaaac ggaagaactt attcgacgtt
      181 tacggcgagc acaatttgcg agacaaaaag ttgaaatttt taaacctcga atagatactc
      241 gttattcagt ggacgaagtg gtgtcgcacg acagaaatac tataccttct acgacggtcg
      301 attcttcggc aagcatttta cttttgagtt cgaacgttga tgtagtaggc attgatgaag
      361 ctcaattttt tgatgatggg ttggtggatg tttgtaatca attggcaaac gatggaatcc
      421 gtgtgattgt agctggattg gatatggatt ttcggggtgt tccttttgga cccatgccag
      481 ctttgcttgc tatagcagac gatgtttaca aagtgcatgc tatttgtgtc cgctgtgggg
      541 cactggctta tatttctcac cgactggttg aaaatgacaa acaagtgttg ctgggtgagt
      601 tggatcagta tgaacccctt tgtcggaaat gttacaatga agttcagaag aagtcttctc
      661 ggcattaaaa aacaagtaat tttatgagcg aaaattcaaa accttatact tttgatcggg
      721 ttgtacgtat tattattacg ttgaccataa tttttttgct cttcctttta ttaaaaaaga
      781 taagtaaggt tttgttgcct tttctcgttg gctggctttt ggcttattta ttacaaccca
      841 ttgttcattt ttttcaacat aagttaaaat tcaagtcacg cttattgtcg gtcattgtta
      901 cccttctgct tttcatagga ttttttgttg gtttgggatt tattcttgtt cctattatta
      961 ttgatgagac ccgaacagtt gttggattaa tcaatagtta tattcaaagt acggatatag
     1021 atgccatttt gcctgtggca tggcaaaacc aattaaaaga atttcttgct aaattcgatt
     1081 tccaaacctt gtttcagaat gaaaattttc tgtcgattct caaaaaattc ttgcctgaag
     1141 tttggaattt tgttagtggt gctctcaatt ttattttgag ttgggcagta attattatta
     1201 tttttcttta tttgtttttt attttaatgg attacgaaag aatttctacc aaatggattg
     1261 aactggttcc tcaaaaatat cgttcaatag tttggcagat agctcaagat atcgcagatg
     1321 gaatgaatcg ctattttaga gggcaagcgc tggtggctat gtgtgtagga attttgcaca
     1381 tgatcgggct aagtattatt ggtttaccga tgtctatttt aatgggcttg actattggta
     1441 ttttaaatct tgttccgtat ctggaattac tgggcattat tccatgtttt tttctggcag
     1501 tattaaagtc agctgaaaca ggtcaaaatt tttggcttgt tgttttaagt attgctattg
     1561 tatggcttgt agtgcaagcc atacaagatt tgattttggt tccaaaaata atgggcaaag
     1621 taacaggact ccatcctgct ttaattttat tgtcgctttc catctggggt gctttattgg
     1681 ggttggctgg aatgataatt gctttgccca ttacttctct cattttatca tattacagac
     1741 gttttattgt attggatgaa aggagaacta cgcactcggg aacttttaat gaagaatggc
     1801 tttcaaagag aaaagataag gatgacagtt cttcctcttg aaaaataata ttatt
//
LOCUS       DAOTKS010000337         1849 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_50692_length_1849_cov_5.046265, whole genome shotgun sequence.
ACCESSION   DAOTKS010000337 DAOTKS010000000
VERSION     DAOTKS010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50692_length_1849_cov_5.046265"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..773
                     /gene="lptB"
                     /locus_tag="P5236_06290"
     CDS             36..773
                     /gene="lptB"
                     /locus_tag="P5236_06290"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HRU64007.1"
                     /translation="MILRTEHLVKKYSKRIVVDDVSIQVGQGEIVGLLGPNGAGKTTT
                     FYMVVGLVKPLAGEIFLNEQNITNFPVYKRAQAGIGYLAQEPSVFRKMTVEDNIKSVL
                     EMTKFSKEYQKEKLETLITEFNLSHVRKNIGDRLSGGERRRTEIARCLAIEPRFILLD
                     EPFAGVDPIAVQDIQDIVWKLKDKNIGILITDHNVDETLAITDRAYMLFDGKILFQGT
                     AKELSENPIVREKYLGRDFELKKKTKV"
     gene            854..1771
                     /locus_tag="P5236_06295"
     CDS             854..1771
                     /locus_tag="P5236_06295"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HRU64008.1"
                     /translation="MPASAKLTILGCGSALPTNKNFPSSFMFDIRDKQFMIDCGEGSQ
                     IRLRQMGLKTNRFGHIFISHLHGDHCFGLLGLISTFSLLHRTAELHIHAHPDLEKIME
                     TQMNYFCLGIPFKVIFHSFDPEINALIYEDRSIKVYTIPLKHKIPTCGFLFEEKPRQR
                     HILREMIDFYKIPISKIPKIKQGEDFVTEEGEVVPNEKLTVENIKPVRFAYCSDTMYD
                     ENIIPLIHGVDCLYHEATFMEEDAARAKKTFHSTARQAAEIAKKAGVKKLIIGHYSAR
                     YTDQSLVLKEALEVFDQAVLGEDMRSYEF"
BASE COUNT          605 a          297 c          333 g          614 t
ORIGIN      
        1 atttacaaaa tttttatttt ctaaataaga taaaaatgat attgcgtacc gaacatttag
       61 ttaaaaaata cagcaaacga attgttgtgg atgatgtttc tattcaagta ggacaaggcg
      121 aaattgtagg tttacttggt cccaatggtg ctggcaaaac taccactttt tacatggtag
      181 taggattagt aaagccgctt gcaggggaaa tttttttaaa tgaacaaaat attactaatt
      241 ttcctgtata taaacgtgca caggcaggta ttggctattt ggctcaagaa ccttctgttt
      301 ttagaaaaat gacggtagaa gacaatatca aatctgtgct tgaaatgaca aaattcagta
      361 aagaatatca aaaagaaaaa ttagagactt taattactga atttaatcta agtcatgtta
      421 gaaaaaatat tggagaccgc ctttctggtg gtgaacggcg acggactgaa attgcccgtt
      481 gcttggctat tgagccacgt tttattctgt tggatgaacc ttttgcaggc gttgatccaa
      541 ttgctgtgca ggatattcag gatatcgttt ggaaactaaa agacaaaaat attggtattt
      601 taattactga ccacaatgtt gatgagacat tagccatcac cgaccgagca tatatgcttt
      661 ttgatggaaa aatcttattt cagggtacag ccaaagaatt gtctgaaaat ccaattgttc
      721 gtgaaaaata tttaggacgc gattttgagt taaaaaagaa aactaaagtt taaacttttt
      781 atcactttcc tcaatattta attctttcac aattgttaaa tgtaaatttt tcgttccttt
      841 atttcttgat attatgcctg cttctgcaaa attaacaatt ttaggttgtg gatcagcctt
      901 accaacaaat aagaattttc ccagctcttt tatgttcgat atccgtgata aacaatttat
      961 gattgattgt ggcgaaggtt ctcagattcg tttacgacaa atgggactca aaaccaatcg
     1021 tttcggacac atttttattt ctcatcttca cggtgaccat tgctttggat tattaggatt
     1081 aatttcaact ttttcattgc ttcatcgcac tgctgaatta catattcatg cccatcctga
     1141 cctggaaaaa attatggaaa cgcaaatgaa ttatttttgt ttgggcatac cttttaaagt
     1201 aatattccat tcctttgatc ctgaaatcaa tgctttgatc tacgaagacc gttcaataaa
     1261 ggtttatacc attcctttga aacataaaat acccacttgt ggctttttgt ttgaagaaaa
     1321 accacggcaa cgacacattc tccgtgaaat gatagatttt tacaaaattc ctatttcaaa
     1381 aattccaaaa ataaaacaag gagaagattt tgtaacagaa gagggtgaag ttgtcccgaa
     1441 tgagaagcta acagttgaaa atataaagcc tgtcagattt gcttattgtt ctgataccat
     1501 gtatgatgaa aacattattc cgcttattca cggagtagat tgtctctatc acgaagcaac
     1561 ttttatggaa gaagatgcgg ctcgagcaaa aaagactttt cattccactg ctcgtcaggc
     1621 ggctgaaatt gctaaaaaag caggagttaa aaaactgatt atcggacatt attcagctcg
     1681 ttataccgac caaagtttgg tgctgaaaga agcattggaa gtgtttgatc aagccgtttt
     1741 gggcgaagat atgcggagct atgaatttta aagataaatt aacttcttat tattaatttt
     1801 ttagcttgaa ttatttacca ttatgcgtat tgacattatt tcagcggtt
//
LOCUS       DAOTKS010000338         1843 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_50929_length_1843_cov_5.384787, whole genome shotgun sequence.
ACCESSION   DAOTKS010000338 DAOTKS010000000
VERSION     DAOTKS010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50929_length_1843_cov_5.384787"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="P5236_06300"
     CDS             <1..1011
                     /locus_tag="P5236_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRU64009.1"
                     /translation="GNSPDPLDLIQKYGADAVRMGMLLCSPAGNDLLFDESLCEQGRN
                     FNNKIWNAFRLIKGWTIVEIEQPMASAKAVKWFESQLNKSLAEIDDLFAKYRLSEALM
                     VAYKLFWDEFASWFLEIIKPAYQEPIDKISYEAAIHFFETLLKILHPFIPFITEELWQ
                     NLTERKEGESIMIQLFPQAGKIDETLLENFESTKQIIANIRAIRLQKNIPNKKPLKLQ
                     IVGKSDPYFDSVIEKLGNLDAIETVSEKSAEAASFLVGTTEFAIPLAGFINVEEEIKK
                     IQKEIDYLEGFLASVTKKLNNQNFLSHAKPEIVESEKKKQSDTEKKLTSLRETLAGLM
                     NS"
     gene            complement(1062..1499)
                     /gene="ybeY"
                     /locus_tag="P5236_06305"
     CDS             complement(1062..1499)
                     /gene="ybeY"
                     /locus_tag="P5236_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRU64010.1"
                     /translation="MMIQYISEGVKVPQIKKQKINRWIKAVAADYDKKIGDIAYIFCN
                     ENRILEINQKYLQHDYYTDIITFDDSERNLISGDIFICIDTVKSNAEKFQTDFMQELY
                     RVMIHGILHLCGQDDKTSEQKEEMTFKENKALSQLKEILSPKI"
     gene            complement(1656..>1843)
                     /locus_tag="P5236_06310"
     CDS             complement(1656..>1843)
                     /locus_tag="P5236_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007902617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate aminotransferase"
                     /protein_id="HRU64011.1"
                     /translation="QGKTINDSAELAMYLLEVGHVASVGGVAFGAPHCIRFSYATSDE
                     NLKIAFARIKEALAQLK"
BASE COUNT          643 a          285 c          298 g          617 t
ORIGIN      
        1 ggcaattcac ctgatccact ggatttgatc caaaaatatg gagctgatgc agtacgaatg
       61 ggaatgttgc tttgttcgcc agcaggaaat gatttgcttt ttgatgaaag tctttgtgaa
      121 caaggcagaa actttaacaa caaaatctgg aatgcttttc gcctgatcaa aggctggaca
      181 atagtagaaa ttgaacaacc tatggcatct gcaaaagctg ttaaatggtt tgaaagtcaa
      241 ctcaataaat cgctcgcaga aatagacgat ttgtttgcaa aatatcgttt gtctgaagct
      301 ttgatggtgg cttataaact tttttgggat gaatttgctt cgtggtttct ggaaattatc
      361 aaaccagctt atcaagaacc tattgataaa atttcttacg aagcggctat tcattttttt
      421 gaaacattgt taaaaatact gcatcctttt attcctttta ttacagaaga actatggcaa
      481 aatttaacag aaagaaaaga gggtgaaagc attatgattc aattatttcc acaggctgga
      541 aaaatagatg aaacattgct tgaaaatttt gaatccacga aacaaattat tgctaatatc
      601 cgggctattc gtttacaaaa aaatattcct aataaaaagc ctttaaaact gcaaatagtg
      661 ggcaaatccg atccatattt tgattcagta attgagaaat tgggtaatct ggatgccatt
      721 gaaacagtga gtgagaaatc tgcagaagct gcttcatttc tggttgggac aacagaattt
      781 gctattccac tggctggttt catcaatgta gaagaagaaa ttaaaaaaat tcaaaaagaa
      841 attgattatc tggaaggatt tcttgcttcg gtaacgaaaa aactgaacaa tcaaaatttt
      901 ctgagtcacg caaaacctga aattgtagaa agtgaaaaga aaaaacaatc tgatacagaa
      961 aaaaaactca cttctttaag agaaacgctt gctggtttga tgaattcata attttttata
     1021 ttctcctgat aataatttga tatacaattt tttaaaagag attaaatttt gggagataat
     1081 atttctttca attgagaaag tgctttgttt tctttgaaag tcatttcttc tttttgttca
     1141 gaagttttat catcctgacc acataaatgg agaatgccgt gaatcattac acgatataat
     1201 tcttgcataa aatctgtttg aaatttttcc gcattacttt ttacggtatc aatacaaata
     1261 aaaatatcgc ctgaaatcaa atttctctca ctgtcatcaa aggtgataat gtctgtataa
     1321 taatcatgct gaagatattt ctgattaatt tccagtattc gattttcatt acaaaaaatg
     1381 taagcaatat ctccgatttt tttatcataa tctgcagcta ctgcttttat ccagcgatta
     1441 attttttgtt tttttatttg tggcactttt actccttctg aaatgtattg aatcataata
     1501 aaaatttaga tgacgtttaa attattttaa aaaagcattt aaaaacaaaa cagtgaaact
     1561 aaaataagaa aatagtttca ctgcatcaac ttttatgaaa aagattttta cttgttttta
     1621 taaaaaactg tttttcttat atttataaca tttagttatt tcaactgtgc caacgcttcc
     1681 ttgatacgag cgaatgcaat tttgagattt tcgtctgacg ttgcataaga aaaacgaata
     1741 caatgaggag caccaaaagc tacaccgcca acagaagcca catgtcccac ttccagcaaa
     1801 tacattgcta actcagcaga atcatttatg gttttgcctt gat
//
LOCUS       DAOTKS010000339         1832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_51394_length_1832_cov_4.129994, whole genome shotgun sequence.
ACCESSION   DAOTKS010000339 DAOTKS010000000
VERSION     DAOTKS010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51394_length_1832_cov_4.129994"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            418..1545
                     /locus_tag="P5236_06315"
     CDS             418..1545
                     /locus_tag="P5236_06315"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRU64012.1"
                     /translation="MKKILSFILPLLILVSSCISRNSHRTGETPLCSLHLCYAKGFSV
                     SYFKNFKEVTVFNPWAKEAVYQRYYLVHDTSVTVPVNGVRIKIPVHTLAATSVTHFEF
                     LRLLNEIDAVTGVCSAKIIYNSQINDRLKKGLVVDLGDAFNMNLEKTVQLHADLVMVS
                     GYNQNDPFAQRLVEIGIPVVYNNEWMENSLLGRAEWIKFIGAFFDKEKLADSIFQKIE
                     QQYLEASNKAKGVKYRPTIMSGSNFRGTWYMPGGQNFMAQLFLDAGGNYFYAGDTTKG
                     SLPLNFEKVLKNFAETDMWLNCSYCSLDELLKADAKHALFRPVKLKQVYNFNKRRLSS
                     GANDFWESGIAHPDLLLKDIISILHPELLPDYELFYTKKLE"
BASE COUNT          609 a          271 c          324 g          628 t
ORIGIN      
        1 acggttacga cacgtacgat tacggagcac gaggaatgta tcccgctatt atgcgattca
       61 ccacccttga tccgctggca gagaagcact ataatatttt tttaagaatt tcataggctc
      121 attcttgaga aaatgacctt aattttgata atatatttta tcaatacagt acgttgtaat
      181 tcagataatt ataattcatt ttttctaaca actgctatta gaaattttac ttaatgacaa
      241 atatgaagta actaaaaaaa ttgctcgtaa gcataaaaaa taaactttta gttttcatgc
      301 tttgcattgg ctgttttcag tttttccagc tatctttgtg aacaacagtg ttgatgttat
      361 aaattttgac tttttgtaag atgggcgtaa catttatgac gatttaagta aattttgatg
      421 aaaaaaatct tgtcttttat attaccgtta ctgattttag tttcctcatg tatttcccga
      481 aattcgcata gaactggcga aacgccacta tgttctttac acctgtgtta tgctaaaggt
      541 ttttcagtta gctatttcaa aaattttaaa gaagtaacgg tttttaatcc ttgggcaaaa
      601 gaagccgttt atcaacgata ttatttagtt catgatacgt cagttactgt accagtgaat
      661 ggtgtgagaa ttaaaattcc tgttcataca ttagcagcta cttctgttac tcattttgaa
      721 tttctgcgtt tactaaacga aattgatgct gttacaggtg tttgctctgc taaaattatt
      781 tacaattccc aaatcaacga cagacttaaa aaaggtcttg tagtagattt gggagatgca
      841 tttaatatga atcttgaaaa aacagtacaa cttcatgcag atcttgtaat ggtaagtggt
      901 tataatcaga acgatccgtt tgcacagcgt cttgttgaaa taggcattcc agtagtttac
      961 aacaacgaat ggatggaaaa ttccttattg ggacgtgcag aatggataaa atttataggt
     1021 gcgttttttg ataaagaaaa actcgccgat agtatttttc aaaaaattga acagcaatat
     1081 ttggaagctt caaacaaagc aaaaggagta aaatatcgcc ctacaataat gtctggttct
     1141 aattttcgag gaacttggta catgccaggt ggacaaaatt ttatggcaca acttttttta
     1201 gatgctggtg gaaattattt ttatgcaggt gatacaacta aaggaagttt gcctttgaat
     1261 tttgagaaag tcctaaagaa ttttgccgaa actgacatgt ggttgaattg cagttactgc
     1321 tctttagacg aattgttgaa agcggacgca aaacatgcac tttttcgtcc cgttaaatta
     1381 aagcaggttt ataatttcaa caaacggagg ttgtcatcag gagctaacga tttttgggaa
     1441 agtggtatag ctcatcctga tttgttacta aaagatataa tttccatttt gcatccggaa
     1501 ttattacctg attatgaatt gttttataca aaaaaactgg aataaaacat tagaatttta
     1561 agccaaaaga aattattagt tttaagttgt caaaatttag ttggtaatca ggagtttggg
     1621 gttctcgaag ggcaaggttt tgattgtgaa aaaaatgaat aattataatt tcgcttgaca
     1681 ggacagcaac aaaagaaaaa tttctatgcc ttctcgatgt gactctgtga ttttttaatt
     1741 tttattattc agaaaacaca caaaaattta cccagaaaag acataaagat ttatttcttg
     1801 ctcttgtgca gtcaaataaa accctaaata at
//
LOCUS       DAOTKS010000340         1818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_51980_length_1818_cov_4.083381, whole genome shotgun sequence.
ACCESSION   DAOTKS010000340 DAOTKS010000000
VERSION     DAOTKS010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51980_length_1818_cov_4.083381"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="P5236_06320"
     CDS             complement(<1..710)
                     /locus_tag="P5236_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRU64013.1"
                     /translation="MKKLAIIGASYLQKPLVLKAKEMGIETHCFAWEEGAVCKDIADF
                     FYPISITEKNLILKKCEEIGIDGITSIASDLASPTMCYVAEKMGLIGNSYSVALVAAN
                     KYLMRKCFADKNVNSPRFIIANENYSLKNFKFPLIVKPTDRSGSRGVMKIKHAEEIEQ
                     AVKRAKNESFSGEVIIEEFISGDEVSVETISWEGKHYILAITDKVTSGEPYFVELEHH
                     QPSMLPSEIQLKIKNLTI"
     gene            complement(707..>1818)
                     /locus_tag="P5236_06325"
     CDS             complement(707..>1818)
                     /locus_tag="P5236_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRU64014.1"
                     /translation="VWALKDINLKVEQGEVLGIIGKNGAGKSTLLKILSRVTSPTTGT
                     IRARGRIASLLEVGTGFHPEMTGRENIYMNGAILGMTKKEIDRKLDEIVDFSGVERYI
                     DTPVKRYSSGMTVRLGFAIAAHLEPEILVVDEVLAVGDAEFQKKAIGKMQDVSKGDGR
                     TVLFVSHNMESIQNLCNNGILLGNGSIIKSGKIEDVVNYYISGNLASTENVGVIIDTG
                     KYRSKGTTKEVEFLYLERVNNYRIDVNDPLIFKMGLRKNANVKKIQLMALIDDISNGG
                     NRVGMSFSEEIILPEHKKEFEVEFTIQHHNLTKSKYIMDFWIGIGDVTSTIKYYDMLY
                     DTLTFEINTCNGKYINEWHNYWGQNCFTSVNVVLK"
BASE COUNT          502 a          406 c          293 g          617 t
ORIGIN      
        1 ttgatcgtta gatttttaat tttcagttgg atttccgaag gcagcatgga tggttggtga
       61 tgttccaatt cgacaaaata aggttctccg cttgtaactt tatctgtaat tgccaaaata
      121 taatgttttc cttcccatga aattgtttca acactcacct cgtcgccgct aataaattct
      181 tcaataatca cttcacctga aaacgattca tttttagcgc gtttcactgc ttgttcaatt
      241 tcttctgcat gttttatttt cataacgcct ctgctgcccg aacgatcagt tggcttcaca
      301 atcaaaggaa atttaaagtt tttaagagaa taattttcat ttgcaataat aaagcggggt
      361 gaattgacat ttttatctgc aaaacatttg cgcatcaagt atttattggc tgcaactaat
      421 gccacagaat aagaattacc aatcaagccc attttttctg ccacatagca catggtaggc
      481 gatgcaagat ccgaagctat tgatgttatg ccatcaatcc ctatttcttc acattttttc
      541 aaaatcaaat ttttttcagt gatggaaata ggataaaaaa aatcggctat atctttgcaa
      601 acagctcctt cttcccatgc aaaacaatgc gtttcaattc ccatttcttt tgctttcagt
      661 accaatggct tttgcaaata acttgcacca ataatggcta attttttcat tttaaaacaa
      721 cgtttacaga agtgaaacaa ttctgtcccc agtaattgtg ccattcgttg atgtacttac
      781 cattgcaggt gtttatttca aaagttaagg tgtcgtacaa catgtcatag tatttgattg
      841 tagaagttac gtcgccaatt cctatccaaa aatccatgat atatttgctt ttggtcaagt
      901 tgtgatgctg aatggtaaat tctacttcaa attccttttt gtgttcaggt agtatgattt
      961 cttcagaaaa cgacatccca actctgtttc ctccattgct tatatcatca attaaagcca
     1021 tcaattgaat tttttttaca tttgcattct tcctcaaacc cattttgaaa ataagcggat
     1081 cgtttacatc aattcgataa ttgtttactc tttcaaggta caaaaattct acttcttttg
     1141 tagtcccttt tgatctgtat ttaccggtat cgattattac tccaacattt tcagttgatg
     1201 ccaaattacc ggatatataa taattgacca catcttcaat cttacccgac ttaataatac
     1261 ttccatttcc cagtaaaata ccattgttgc ataaattctg aatactttcc atattatgac
     1321 tcacaaacag cacggttcgg ccatcgcctt tgctcacgtc ctgcattttg ccaatggctt
     1381 ttttctgaaa ttcggcatcg cctacggcca gcacttcgtc caccaccaga atttcaggtt
     1441 ccaaatgggc ggcaatggca aatcccagtc gcaccgtcat accgctggaa taacgtttca
     1501 ccggtgtatc gatgtaacgt tccacgcccg agaagtccac aatttcgtcg agtttacggt
     1561 caatttcctt tttggtcata cccagtatgg cgccgttcat ataaatattt tcgcgtcccg
     1621 tcatttcggg atgaaaacca gtgcccactt caagcaggga agcaatgcgc ccgcgtgcgc
     1681 gaatggtccc ggtagtgggt gaagttacgc gcgatagaat ttttagcaaa gtagattttc
     1741 cagcgccgtt tttccctata atgcccaaca cttcgccctg ttccactttc agattaatgt
     1801 ctttcaacgc ccatacat
//
LOCUS       DAOTKS010000341         1816 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_52053_length_1816_cov_12.412266, whole genome shotgun
            sequence.
ACCESSION   DAOTKS010000341 DAOTKS010000000
VERSION     DAOTKS010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52053_length_1816_cov_12.412266"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..>1816)
                     /locus_tag="P5236_06330"
     CDS             complement(177..>1816)
                     /locus_tag="P5236_06330"
                     /inference="COORDINATES: protein motif:HMM:NF025943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chondroitinase-B domain-containing protein"
                     /protein_id="HRU64015.1"
                     /translation="KPILIKSKNLFGAKITGASYFNPQGQSYVTYEGFKFELSPVSTI
                     FKMEGCSYVRITRNWFTMNSDATKNSKWITIGEIWENEICRSHHNRIDHNLFEGKHDI
                     GALLVIDGSHGTVPAISQHDKIDHNIFRDNTPRVDNEKETIRIGVSDLSNLNAYTVVE
                     YNLFENCDGDPEIISIKSNCDTIRHNTFRRCLGTICLRQGRGSVVEGNFMFGENKTAL
                     FNGGIIGCGGVRMYGLNHKIFNNYFEELTGDKWDAALTITNGDVTNSSTSLSSHFLPE
                     NIIITNNTLINNVSNIEIGFDNNGSYGKSPKNCVIANNIVSASSNPIVKYYSTTSLAG
                     VSFTNNIFYPTEAASVGLPSYNDSQLRTIDPELVLTNCRAYNQNCANMIPFNLFKLTS
                     FSPAVNASTAYDFVTTDFEGQPAVGIRDIGADEYNTVSLVVNGPMDETSAGPEAPENY
                     TYELNEIVGWKNIVNNEISVMPNPFNGSTQLMISNTKAGKITVILYNILGEKIQQNEI
                     ETNEGMIQIKISTKQKGLLFCQISSANKSYVLKLISK"
BASE COUNT          538 a          331 c          288 g          659 t
ORIGIN      
        1 ttccatacgt tgtccaacct gaattatctt ttagtttact accctctgga tagttaaatg
       61 tttcatctag ataaacggtt ccatttgcca taaatgtcaa ggcaatcatt gtgataagta
      121 atgtaatttt tttcataatc attaaattta aatagttaaa cttttaaagt gaatttttat
      181 ttagaaataa gtttcaaaac atatgattta tttgctgaac taatttggca gaatagcaaa
      241 cctttttgtt ttgttgaaat ttttatttgt atcattcctt cgtttgtttc tatttcattt
      301 tgctgaattt tttcccccag tatattataa agtattactg ttattttccc tgcttttgta
      361 ttggatatca ttaattgtgt actaccgttg aaaggattag gcattacaga aatttcatta
      421 tttacaatgt ttttccatcc tacaatttca tttagctcat aggtataatt ttcaggtgct
      481 tctggtcctg cacttgtttc atccattggt ccgttaacga ccagagaaac agtattgtat
      541 tcatctgctc cgatgtctct tattcccact gctggttgtc cttcaaaatc ggttgtaaca
      601 aaatcataag ctgtggaagc attcactgct ggactgaaag atgtcaactt aaagagattg
      661 aaaggtatca tattagcaca attctgattg taagctcgac aattcgttaa aacaagttct
      721 ggatcgatcg ttctcaattg tgagtcattg taactcggca atccaactga agcagcttct
      781 gtcggataaa agatattatt ggtaaaactg actcctgcca aagaagttgt actataatat
      841 tttactatcg gattagacga tgccgaaaca atgttgtttg caatcacaca atttttcggt
      901 gattttccat aagaaccatt attatcgaaa ccaatttcta tattcgatac attgttgatt
      961 aacgtattgt tggtaatgat aatattttca ggtaaaaaat ggctggacag gctggtagat
     1021 gaattggtta cgtctccatt agtgatggtt aaggcagcat cccatttgtc accagtaagt
     1081 tcttcaaaat aattgttgaa gattttgtgg tttaatccat acatccttac accaccacat
     1141 ccaataattc ctccattgaa aagtgcggtt ttattttctc caaacatgaa attaccctcc
     1201 actacactac ctcggccttg acgtaaacaa atggtcccaa ggcaacgtcg aaaagtattg
     1261 tgccgaatgg tatcacaatt gctttttatc gaaataattt caggatctcc atcgcaattt
     1321 tcaaataaat tatattccac taccgtataa gcattgaggt tgctcaaatc gcttacaccg
     1381 atacggattg tttctttttc attatccacg cgaggcgtat tatctctaaa aatattatga
     1441 tcaatcttgt cgtgttgtga aatagcagga actgttccat gactaccatc aatgacaagt
     1501 aacgctccta tatcatgttt gccttcaaaa agattatgat caattctgtt gtgatggctt
     1561 ctgcaaattt cattctccca tatttcgcct attgttatcc atttcgagtt ttttgtagca
     1621 tcagaattca tggtaaacca gtttctagta atacgcacat aactgcaacc ttccattttg
     1681 aaaatagtgc ttactggact gagttcaaat ttaaaaccct cgtaagttac atagctttgt
     1741 ccctgtggat tgaaataaga agcaccagta atttttgctc cgaaaagatt tttcgatttt
     1801 attaaaattg gtttta
//
LOCUS       DAOTKS010000342         1810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_52323_length_1810_cov_2.196581, whole genome shotgun sequence.
ACCESSION   DAOTKS010000342 DAOTKS010000000
VERSION     DAOTKS010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52323_length_1810_cov_2.196581"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="P5236_06335"
     CDS             <1..976
                     /locus_tag="P5236_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007480308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-N-acetylglucosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HRU64016.1"
                     /translation="TWKVHEGFYGKNWIFSYTPNFGGKTPLTGELTLYASYSIQALQS
                     SYRNNLIGFGFAPEGIENNEVVYELLADMGWQDKEMDINNWIAKYCMAKYGGYPKKME
                     KAWKLLLQSVYSSLYSYPRFTWQTVVPDQRRKSKIDTSEKFLQGVELFLDCSDELKKS
                     PLYCNDAIEFSALYLSAKADFCYKKALKTDSLNQKAEAKKALDQTIDILLFVDKLLAS
                     HPTHKLENWVNFTHKQATNDAEKMKYEADAKRLITTWGGFQEDYAARVWNGLIRDYYI
                     PRIQIYFSNKRNSLNDWEEEWINTPWQPQVKPFSNPIEEAKKYIKQLK"
     gene            988..>1810
                     /locus_tag="P5236_06340"
     CDS             988..>1810
                     /locus_tag="P5236_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylglucosaminidase TIM-barrel
                     domain-containing protein"
                     /protein_id="HRU64017.1"
                     /translation="MKRAKFFLVIFFVGLALLVNATPQSNMQEQKKAVQDLIKRVLPQ
                     KANDFEIEFISKDSDDKDIFEVFSKNDKIILRGNNGVSVASAFNYYLKHIAHCQITWN
                     GTNLNLPEPLPKVDQLIRQKSPYKYRYYLNYCTFNYSMSWWDWERWQKEIDWMAMNGI
                     NMPLALTGQNSIWREVYKSLGFSDEDLKDFFSGPAYFSWFWMGNLDGWDGPLPLSFMQ
                     KQEELQKKILQQERSLGMTPILPAFTGHVPPSFQEKFPQAKIRKTSWVNFPEVCIL"
BASE COUNT          636 a          275 c          334 g          565 t
ORIGIN      
        1 gacatggaaa gtacatgaag gattttatgg gaaaaactgg attttttcct atactccaaa
       61 ttttggtgga aaaactccat tgaccggaga attaactctc tacgcttctt attctataca
      121 agctttacaa tcttcttatc gaaataattt aattggtttt ggctttgctc ctgaaggaat
      181 agaaaacaat gaagtggtat atgaattatt ggccgacatg ggttggcaag acaaagaaat
      241 ggatattaat aactggatag ctaaatattg catggccaaa tatgggggtt acccgaaaaa
      301 aatggaaaaa gcatggaaac ttttacttca atctgtttat agttcgttgt attcttatcc
      361 tcgatttaca tggcaaacgg ttgttcccga ccaacgcaga aaaagtaaga tagataccag
      421 tgaaaaattt ttgcaaggag tcgaattatt tttggattgt tctgacgaat taaaaaaatc
      481 gcctttatac tgtaacgacg ctattgaatt ttcggcttta tatttatcgg caaaagcaga
      541 tttttgctat aaaaaagcat taaaaacaga ttctttaaac caaaaagcag aagccaaaaa
      601 ggctttagat caaactatcg atattctttt gttcgttgat aaactgttgg cttcgcaccc
      661 tacacataaa cttgaaaact gggtaaattt cacacacaag caagctacca atgatgctga
      721 aaaaatgaaa tatgaagctg atgctaaacg attaatcact acttggggtg gttttcaaga
      781 agattatgca gctcgagtgt ggaatggact tattcgtgat tattatattc ctcgtattca
      841 aatttatttt tcgaataaaa gaaattcatt gaacgattgg gaagaagaat ggataaatac
      901 tccgtggcag cctcaagtaa aacctttttc aaatccgatt gaagaagcaa aaaaatatat
      961 taaacaatta aaataaattt cggtaaaatg aaacgggcaa aatttttctt agtaatattt
     1021 tttgtagggt tagcattatt agttaatgct acgccacaaa gcaatatgca agaacaaaaa
     1081 aaagcagttc aagatttgat aaaaagagtt ttaccacaaa aagctaatga ttttgaaatt
     1141 gaatttatta gtaaagattc cgacgacaaa gatatttttg aagtattttc gaaaaatgat
     1201 aaaattatct tgcgtggaaa caacggagta tcggttgcaa gtgctttcaa ttattattta
     1261 aaacacattg cccattgtca aattacgtgg aatggcacca atttaaactt accagaacct
     1321 ttacccaaag tagatcagct gataagacaa aaatcgcctt acaaatatcg ttattatttg
     1381 aactactgta cttttaatta cagtatgagc tggtgggatt gggaacgctg gcaaaaagaa
     1441 attgattgga tggcaatgaa tggcatcaat atgcctcttg ctttaacagg tcaaaattct
     1501 atttggcggg aagtttataa aagtttaggt ttttctgatg aagatttaaa ggattttttc
     1561 agtggaccgg cttattttag ctggttttgg atgggtaatc tcgacggttg ggatggaccc
     1621 ttgcctttat catttatgca gaaacaagaa gaattgcaga aaaaaattct tcaacaagaa
     1681 cgttctttgg gaatgacacc tattttgcct gcttttacag gacatgttcc tccttctttt
     1741 caagaaaaat ttccacaagc aaaaatcaga aaaacttcat gggtaaattt tccagaagtg
     1801 tgtattctcg
//
LOCUS       DAOTKS010000343         1805 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_52508_length_1805_cov_2.272571, whole genome shotgun sequence.
ACCESSION   DAOTKS010000343 DAOTKS010000000
VERSION     DAOTKS010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52508_length_1805_cov_2.272571"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /gene="cmr4"
                     /locus_tag="P5236_06345"
     CDS             complement(<1..655)
                     /gene="cmr4"
                     /locus_tag="P5236_06345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr4"
                     /protein_id="HRU64018.1"
                     /translation="MNQLFYKIHCKTNLHAGSGKANYGVIDQLVQRDPTYQYPTIHAS
                     SLKGAIKEYCYHIRFKEMKEVFGSDKGTDGRTNQEEAGIGSYKFFDANLLAIPVRCDK
                     QAFVHITCPNILTELNERLPNQCELKQALFTFINELSNEMPIAITFSENLKKAIIEDF
                     DIQAQYEENIQLDSCIKELFNGYEIVIVKNNYFDRLVNDLHLPVIARNYLENGESQNL
                     "
     gene            complement(652..1650)
                     /locus_tag="P5236_06350"
     CDS             complement(652..1650)
                     /locus_tag="P5236_06350"
                     /inference="COORDINATES: protein motif:HMM:NF021236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module-associated Cmr3 family
                     protein"
                     /protein_id="HRU64019.1"
                     /translation="MSKTYFVTLTPYQLYFFGREQEEVADYYLKGNPLPQQTALLGMV
                     RYQILKQNNLLKNNKVKDAKEANDWIGEKSFQYTDKNMDFGKIISLSSCYIVKTDCDD
                     SKKYLPTYPKFFHDLRKIGDNYFLPCYDPKKDYALRWRNIKDQKDTLEDNFYEEVERV
                     GINKNYEGKTEEDGFYKQVWFKMKKRYAFGFYLTLSDESVTFHDADVNLGKENAVFRM
                     NVMEDTLPEESHNNADNAVVLTCDAFIEYSILDKCLFAACDNVPFRNLCNKTDLTHNH
                     YGLHKNNKRIQLIKRGSAFYLDDADKVNSFIQALNDEKNFKKIGYNSFQTFKLNIL"
BASE COUNT          547 a          328 c          275 g          655 t
ORIGIN      
        1 acaagttttg actttcacca ttttccagat agttgcgagc aattaccggc aaatgtaaat
       61 cattgacgag cctgtcaaaa taattgtttt ttactataac tatttcatat ccattaaata
      121 gttcttttat acacgaatct aattgtatat tttcttcata ttgagcctga atatcaaaat
      181 cttcgataat agcttttttt agattttcag aaaatgtaat tgctatgggc atttcattac
      241 ttaattcatt aatgaaagta aacaaagcct gttttaattc acattgattc ggaagccttt
      301 cgttcaactc tgttaaaata ttgggacagg taatgtgcac aaatgcttgc ttatcgcatc
      361 gaacaggaat agccaacaaa ttggcgtcaa aaaatttata ggacccgatt cccgcttctt
      421 cttgatttgt tcttccatct gttcctttat cggagccaaa aacctctttc atttctttaa
      481 atctgatgtg ataacaatac tctttaatgg cacctttaag cgagctggcg tgtatggtgg
      541 gatactgata agtaggatca cgctgcacta actggtcaat tacaccatag ttagcctttc
      601 cacttcctgc atgcaaattg gttttgcaat gaattttata aaaaagttgg ttcataaaat
      661 attgagttta aatgtttgaa atgaattata tcctatcttt ttgaaatttt tttcatcgtt
      721 taacgcttga ataaaactgt ttaccttatc ggcatcatcg aggtagaatg ctgatcctcg
      781 tttaatcaac tgaatgcgtt tgttgttttt atgtaaccca taatgattgt gagtcagatc
      841 tgttttgttg catagattgc gaaacggaac attatcgcat gcagcgaaaa ggcatttatc
      901 tagaatggag tattcaataa aagcatcgca ggttagaaca acagcattat ctgcattgtt
      961 gtgtgattct tcaggcaagg tatcttccat gacgttcatg cgaaaaactg cattttcttt
     1021 acccaaatta acgtcagcat catgaaaagt tacactttca tctgaaagag tcaaataaaa
     1081 accaaaggca tacctctttt tcattttaaa ccatacttgt ttataaaaac catcctcttc
     1141 cgttttccct tcataatttt tattgattcc cacccgttca acttcctcat aaaaattatc
     1201 ttccaaggta tctttttgat ctttaatatt tctccacctc agagcataat cttttttagg
     1261 atcatagcat ggaaggaaat aattatcgcc aatttttctc agatcatgaa aaaatttcgg
     1321 ataagtgggt agatattttt ttgaatcatc acagtctgtt tttactatat aacacgaact
     1381 taaagatatg attttaccaa aatccatatt tttgtcggta tattgaaaac ttttctctcc
     1441 gatccagtcg ttagcttctt ttgcatcttt aactttatta ttcttcaata aattattttg
     1501 ctttaggatt tgatacctga ccatacccaa taaagcagtt tgctgaggaa gtggattccc
     1561 tttcaaataa taatcggcaa cttcttcctg ttctctgccg aaaaaataaa gttgataggg
     1621 agttaaggta acgaaataag ttttactcat cgctttcatc aattaaatgt ttcataaatt
     1681 ggagggtact gaataatttc ccaatgttac ttgtatcggc ttccgtttct ttttcgggta
     1741 aagggacatt atcttttggg aaatccattg ccaatcggca ataataatct actacctctt
     1801 taata
//
LOCUS       DAOTKS010000344         1802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_52592_length_1802_cov_15.273612, whole genome shotgun
            sequence.
ACCESSION   DAOTKS010000344 DAOTKS010000000
VERSION     DAOTKS010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52592_length_1802_cov_15.273612"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..1636
                     /locus_tag="P5236_06355"
     CDS             92..1636
                     /locus_tag="P5236_06355"
                     /inference="COORDINATES: protein motif:HMM:NF019113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HRU64020.1"
                     /translation="MKKTVSILLLIVLFTSLYAAPFRNDSIYSKWAIDSRMGDFRNKK
                     TTQRFLTPTTSRNASWDYVTGLVAKAILKAWEQYKSEPWSAYFFTGIEDFADNVSMKL
                     GESNLDDLNAGKIFFELYRGAMEKGQTSKAETYKTKATTCRNTLKYNHQRIGANLPGT
                     GGFWHKKQYTNQMWLDGLYMGPALYAEWQGNFGQELGETDNTESWDDIALQFDTIFKH
                     TWDVNKKLNYHAWSAEPTNAASSYWADPVTGCSQEFWGRGMGWFFAALVDVLEFMPDS
                     HPAKQRLIAYINAVADGLKARQDPISGCWYQLLQYDGTKKGTTCQVSNYLESSASCMI
                     TYAYLKGMRLGILDKNVYRQMAEKAYKGVIAQFVVEESDGKIKLIQSCESAGLSSTRK
                     GDANYYLCGSDVGINNDTEGKVLGPFIMASLEYERSRQTYTLMKNVQSAQPHCIIKKY
                     GDTVEISSTQQPVQTVCVFDCLGNEKFYWKGKETDQINLNLAGLPQGIYFFKINADVV
                     KYLFTK"
BASE COUNT          631 a          276 c          368 g          527 t
ORIGIN      
        1 ttataaaggt tatgtgtttt aaataaggtt ttaaaaacaa atcaataaaa gattaaaaaa
       61 taagtgtgaa acttaattaa attatttttc gatgaaaaaa actgtatcaa tattattatt
      121 aattgtttta tttacaagct tatacgctgc tccttttcga aacgattcca tttattccaa
      181 atgggcaatt gattctcgta tgggcgattt tagaaataaa aaaactaccc aacgttttct
      241 tacacctacc acttcacgta atgcttcgtg ggattatgtg acgggcttgg tggcaaaagc
      301 cattctcaaa gcatgggaac aatataaatc agagccgtgg agcgcttatt tttttacagg
      361 aattgaggat tttgctgata atgtctcgat gaaacttggc gaaagcaatc ttgacgactt
      421 gaatgccggt aaaatatttt tcgaacttta ccgaggtgct atggaaaagg ggcaaacttc
      481 taaagcagaa acctacaaaa cgaaggctac aacttgtaga aatactttga aatacaatca
      541 tcagcgtatt ggagctaatt taccaggaac aggaggattt tggcataaaa aacaatacac
      601 caaccagatg tggctcgatg gattatatat gggaccagcc ctttatgccg aatggcaagg
      661 aaattttgga caagagctgg gagaaacaga taatacagaa tcgtgggacg atatagcttt
      721 gcagtttgat acgattttta aacatacttg ggacgttaac aaaaaattaa actatcatgc
      781 gtggtcggct gaaccaacaa atgctgcttc ttcttattgg gcagatcccg tgacaggttg
      841 ttcacaggaa ttttggggac gtggtatggg atggtttttt gccgcattgg tagatgtgct
      901 ggaatttatg cccgattctc atcctgccaa acaacgattg atagcttaca tcaatgctgt
      961 tgctgacgga ttgaaagcca gacaggaccc tatttcagga tgctggtatc agttgctgca
     1021 atatgatggt acaaaaaaag gtacaacctg tcaggtgagt aattatctgg aaagttctgc
     1081 ttcttgtatg attacttacg cttatttaaa aggaatgcga ttgggtatac tggataaaaa
     1141 tgtttatcgg caaatggctg aaaaagctta taagggagta atagcccaat ttgttgttga
     1201 agaaagtgat gggaaaataa aacttattca aagttgcgaa tctgctggat taagcagcac
     1261 ccgcaaaggt gatgccaatt attatctctg tggaagcgat gtgggaatta ataatgatac
     1321 agaaggcaaa gtgctcggtc catttatcat ggcaagcctg gaatatgaaa ggagcagaca
     1381 aacctatacc ctgatgaaaa atgtgcagtc tgctcaaccc cattgtatca ttaaaaaata
     1441 tggcgataca gtagaaattt cttctactca acaacctgtt cagactgttt gtgtatttga
     1501 ttgtttgggg aacgaaaaat tttattggaa gggcaaggaa acagatcaga taaatttgaa
     1561 cctggcgggc ctgcctcagg gaatttattt ttttaaaatt aatgcagatg ttgtgaaata
     1621 cctttttacc aaataaaaac aagtctaatc attaaaaaaa caggaaagga ggcaaaatca
     1681 aaaatggtta ctatgtaaaa attaacaaca aagctaatga aaaaattaaa aaaattaaaa
     1741 ttaaaattat gttaaatctt ttaaaaatca atgattatga aacgaattat gaataaaatt
     1801 tt
//
LOCUS       DAOTKS010000345         1800 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_52710_length_1800_cov_2.718625, whole genome shotgun sequence.
ACCESSION   DAOTKS010000345 DAOTKS010000000
VERSION     DAOTKS010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52710_length_1800_cov_2.718625"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1743
                     /locus_tag="P5236_06360"
     CDS             <1..1743
                     /locus_tag="P5236_06360"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HRU64021.1"
                     /translation="ESVLSGSGAGKTFLENLSVGQTVKVNMNIFTLKNSNPVPAKEMI
                     TGNALVLKDGILTVRNTNEVYNSQLYPRTGIGMSQDGKKVFLIVIDKYGKSVGANTTT
                     MCGILKSFGAWNVASMDGGGSAQMMLNGNIVNNPADGKERAVANGWFVYHNAPEDNTI
                     TKIEFADYKIEVPCFASYQPVILGYNQYGVLVNENLSGVKLSCDEGLGHIENENIFVA
                     GANTQNGLLTANYNGIMVSKSINTIPSEIFLRLDSVLVDHRTQYPIEVLSQNDKTVMK
                     IPPSALSWTIRDTEICSIDENGRLKGLKNGSTTVVGTLGDFKDSLKVIVEIPTSGRMI
                     GDNFDVENWTLTASSPLNAVLNTENLPSNWTYGAAVNYVYTSTRAPFIKLLRNAPMYG
                     LPDTLKLIVNVGDINITKAILSLRANNVTVSASKEFTTIPKNTDTELSVAMSELFDAT
                     DIAIYPVWFDNINFYLGTQTSGQAYTLALKEIVLCYQGVEISGIPSVLLSQYEVYPNP
                     LDGTILNIRTKNNDANLLQAQLFHLNGQCVVSQQLNPVNGMATFKVGKLPAGTYLLKI
                     YSDDNPETIKLIVK"
BASE COUNT          616 a          315 c          325 g          544 t
ORIGIN      
        1 gaatcagttc tttcaggtag tggagcgggt aaaacttttc tagaaaattt atcagtaggc
       61 caaacagtaa aagttaatat gaatattttt acattaaaaa actccaaccc tgttcctgca
      121 aaagaaatga taacgggcaa tgctttggtt ttaaaagatg ggatattaac tgtacgcaat
      181 acaaatgaag tttataattc tcaactttat ccacgtacag gtattggcat gagccaagat
      241 gggaaaaaag tttttttaat agtaattgac aaatacggga aatctgttgg agcaaacaca
      301 actaccatgt gtggtatttt aaaatcattt ggtgcctgga atgttgcatc aatggatggc
      361 ggtggatctg ctcaaatgat gttaaacggc aatatcgtaa acaatccagc agacggaaaa
      421 gaaagagcag ttgccaatgg ctggtttgtt tatcataatg ctccagaaga caacacaatt
      481 acaaaaattg aatttgctga ctataaaata gaagttcctt gttttgcttc gtatcaaccc
      541 gtcattttag gatacaacca atatggagtt ttagttaatg agaacttatc aggagtcaag
      601 ctatcatgtg atgaaggatt aggacatata gaaaatgaaa acatttttgt tgctggagca
      661 aatacacaaa atggtttact aacagctaat tataatggta tcatggtatc caaatcaata
      721 aacacaatac cttcagaaat atttctccgt ttagattcag ttttggttga tcatcgcact
      781 caatatccca tcgaggtatt aagccaaaat gataaaactg tcatgaaaat ccctccctca
      841 gctttgagtt ggactattcg tgacactgaa atttgttcca ttgatgaaaa cgggcggtta
      901 aaaggtttga aaaatggcag tacaacagta gttggtactt tgggcgattt caaagactca
      961 ctcaaagtaa ttgttgaaat tccaacttcg ggaagaatga taggtgataa cttcgatgtt
     1021 gaaaactgga cattgactgc ttcctctcct ttaaatgcag tacttaacac ggaaaattta
     1081 cccagcaatt ggacatatgg agctgccgtc aattatgttt atacttccac cagagctcct
     1141 tttatcaaac ttttgagaaa cgctcccatg tacggattgc ccgatacatt aaagctaatt
     1201 gtcaacgttg gagatatcaa tattaccaaa gctattttat cactgagagc aaataatgtt
     1261 actgtttctg cttcaaaaga atttactaca attccaaaaa acacagatac agaactatcg
     1321 gtagccatga gcgaactttt cgatgccaca gatattgcca tttatcctgt gtggttcgat
     1381 aacattaact tttatcttgg gacacaaacc tcaggacaag cttatacact ggctctaaaa
     1441 gaaattgttc tttgttacca gggagtggaa atttcaggaa ttccttccgt attgctttct
     1501 caatatgaag tttatccgaa tccattggat ggaaccattt tgaacatacg aacaaaaaat
     1561 aacgacgcca atcttctaca ggctcaacta tttcacttaa atggtcaatg tgtggtttct
     1621 caacaactga accctgtgaa tggaatggct acttttaagg ttggtaaatt acctgcaggc
     1681 acctatttgt tgaaaattta ttcagatgac aaccctgaaa cgataaaact aatagtaaaa
     1741 taacataaaa aacaatgaaa aaaattcttt ctgcattttc tggattaata attgcattat
//
LOCUS       DAOTKS010000346         1799 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_52744_length_1799_cov_4.033830, whole genome shotgun sequence.
ACCESSION   DAOTKS010000346 DAOTKS010000000
VERSION     DAOTKS010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52744_length_1799_cov_4.033830"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1498
                     /locus_tag="P5236_06365"
     CDS             <1..1498
                     /locus_tag="P5236_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HRU64022.1"
                     /translation="NDGVPSSGNEFILKQVLRKEWGFNGFVVSDWASIKEMINHGFAA
                     DEKEAAMKAVNAGVDMEMVSLTYITYLKELIKEGKVKEKTINDAVRNILRIKFRLGLF
                     DHPYVDENQSSVIYAPSHLATAKQLAVESTILLKNAHNILPLTDEVKTIAVIGPMADA
                     PYEQMGTWVFDGDKAYTKTPLNALKEEYGNKINIIYESALAYTRDKNTEGIAKAVKAA
                     EQADVVLAFVGEESILSGEAHCLADLNLQGAQSQLIEALSSTGKPIITIVMAGRPLTI
                     GKEAALSSAVLYSFHPGTMGGPAIVDLLFGKEVPSGKTPITFPKMVGQIPIYYAHTNT
                     GRPPTGTETMLDQIPVEAGQTSLGNTSFYLDAGFEPLYPFGYGLSYTTFEYSNIKLSS
                     YELKKNDILKISFDLKNTGKYAATEVAQLYIQDKVGSVTRPVKELKRFARVTLQPGEK
                     RTVTFELPISELAFWNIDMKKVVEAGEFNLWVAGDSQSGEKISFKIVD"
BASE COUNT          602 a          264 c          355 g          578 t
ORIGIN      
        1 taatgatggt gtgccttcat ctggcaacga atttattttg aaacaagttt tgcgaaaaga
       61 atggggattt aatggttttg tcgtctctga ttgggcttct ataaaagaaa tgattaatca
      121 tggatttgct gctgatgaaa aagaagctgc aatgaaagca gtaaatgcag gagtagatat
      181 ggaaatggta agcttaacgt atataactta tttgaaagaa ttgattaaag aaggtaaggt
      241 aaaagaaaaa actattaatg atgctgtacg caatatttta cgaattaaat ttcggttggg
      301 gctttttgat catccttacg ttgacgaaaa tcaatcatca gtaatatatg ctccatctca
      361 tttagctact gcaaagcaat tagctgttga atcgactatt cttttaaaaa atgctcacaa
      421 tattcttcct cttactgatg aggttaaaac aattgcagta attggtccaa tggctgatgc
      481 accttacgaa caaatgggga catgggtatt tgatggagat aaggcataca ctaaaactcc
      541 gttaaatgct ttaaaagaag aatatggaaa taaaattaat atcatttatg agtcagcgtt
      601 ggcatatact cgcgataaaa atacagaggg tattgcgaaa gctgtaaaag ctgctgaaca
      661 ggccgacgta gtattagcct ttgtgggcga agaatctatt ctttcaggtg aagctcattg
      721 tttagctgat ttaaatttac agggtgccca atcgcaatta atagaagcat tatcgagtac
      781 aggcaagcct attataacaa ttgtcatggc aggaagacct ttaactattg gaaaggaagc
      841 agctctttca tcagcagttt tatattcttt ccatcctgga acaatgggag gtccagctat
      901 tgtagattta ttatttggga aagaagttcc tagtggaaaa actcctatca ctttccctaa
      961 aatggttgga caaattccga tttattatgc tcataccaac actggacgtc ctccgacagg
     1021 aactgagacc atgttggatc aaattccagt tgaagccgga caaacttcac ttggaaacac
     1081 ttctttttat ttagatgctg gatttgaacc tctgtatccc tttggttatg gcttgtctta
     1141 tacaactttt gaatatagta atataaaact ttcttcgtat gaattaaaaa agaatgatat
     1201 attaaaaata agttttgatt tgaaaaatac aggaaaatat gccgcaactg aagttgctca
     1261 gctttatatt caagataaag tgggctcggt aaccagacca gtcaaagaat taaaacgttt
     1321 tgctcgtgta acattgcaac cgggagaaaa acggactgta acttttgaac ttccgattag
     1381 tgaacttgct ttctggaata ttgatatgaa aaaagtagtt gaagctggtg aatttaattt
     1441 atgggttgcc ggagatagtc aaagtggaga aaaaatttca tttaaaatag tagattaatt
     1501 gttaatgtta agtagtggtt gttggttgct aagttaatta ttaagtggtt ggtttttttg
     1561 tttttcatag gtggtgaagt gaaaagcagg aaggttaaga agacttcctg ctttttgaat
     1621 ttttatacca cgttacaatc aaatataagg cgtataagtc aaaaagtaga ctgaaaattt
     1681 tgcttaagta gtatttcata aacccaaatt aataccaaac cgctatcaaa gtgcatcctt
     1741 aatccaatga tataaacaga ctgattttac cgtttcttta tccagatttt ttaattgtt
//
LOCUS       DAOTKS010000347         1794 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_52987_length_1794_cov_2.677401, whole genome shotgun sequence.
ACCESSION   DAOTKS010000347 DAOTKS010000000
VERSION     DAOTKS010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52987_length_1794_cov_2.677401"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="P5236_06370"
     CDS             <1..183
                     /locus_tag="P5236_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU64023.1"
                     /translation="DYSIVAERWWWNEFLGFYNTTQYLIGLLGKKQTFKLIPSIAFKY
                     YATKVLSKIKRLIKKS"
     gene            180..1259
                     /locus_tag="P5236_06375"
     CDS             180..1259
                     /locus_tag="P5236_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU64024.1"
                     /translation="MNTKLVYVLTSAPEHNYIEQALLSVFTARYHNPDASIVLITDDR
                     TNELLKGQRGEILDYISEKIVVPLPEEMSPMLRSRQLKTSVRNLIEGDFLFIDCDTLI
                     TRPLNEIDNFSFEMGAVLESHLPIGLFINSLYEKVEKNARLLGWSIQNEKNYFSSGVI
                     YVKDTLNNRLFYKKWNHFWNEGLKKGVMIDQPSFAKTNIESGYPVQLLDNRWNCVMYT
                     HPLFDREAYILHFCSFRNMSYIFAEKFLEKVRQQGIKNNSFVQYSILHPHYSYLPFEN
                     AIYRYKPKDYIRMAKEIRQTAKLLKENLDFDDYIPSAGIYRYAGKCFKNKCYFWGSLL
                     ITCYKLYRVKFDKKFKYVENTCAAE"
     gene            1231..>1794
                     /locus_tag="P5236_06380"
     CDS             1231..>1794
                     /locus_tag="P5236_06380"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HRU64025.1"
                     /translation="MLKTPVRLNNKGTGYVKDINNIPMTGERDVHLDVLRILAAFFVI
                     MVHVAGTGLLKGRDYELGSLNWYICMIFSSLARWSVPVFVMISGSCFLPPTKTITLKK
                     LFGKYILRVFVALVVWSSFYAYFFHGKYIPLGLNSGNHLWYLTMIIGIYLALPILKVI
                     PTVLLKYFTLIWFIFLTIDFINKLSGNA"
BASE COUNT          587 a          281 c          322 g          604 t
ORIGIN      
        1 gattattcaa ttgtggcaga acgatggtgg tggaatgaat ttttgggctt ttataatact
       61 acccagtact tgattgggtt gttaggaaaa aagcaaactt ttaaattaat accatcgata
      121 gcattcaaat attatgccac caaagtactt tctaaaataa aaagactgat aaaaaaatca
      181 tgaataccaa actcgtctac gttttaacct ccgcccccga acataattat attgagcaag
      241 ctttgttatc ggtttttaca gcacgatacc ataatcccga tgccagtatt gtactgataa
      301 cggacgatcg taccaatgaa ttgttgaaag ggcaaagagg tgaaatactg gattatattt
      361 cggaaaaaat tgtcgttcct ctgccggaag aaatgtcgcc catgttgcgt tcccgacaat
      421 tgaaaacttc agtcaggaac ttaatagaag gtgattttct gtttatcgat tgtgatacat
      481 tgataactcg tcctttaaat gaaatagata atttctcttt tgaaatggga gcggttttag
      541 aatcccattt acccattggt ttatttataa attctcttta cgaaaaagta gaaaaaaatg
      601 cccggctttt gggatggtca atacaaaatg aaaaaaacta tttcagcagt ggtgtcatat
      661 atgtaaaaga caccctaaac aaccgacttt tttataaaaa atggaatcat ttttggaacg
      721 aaggtttaaa aaaaggagta atgatcgacc aaccatcttt tgccaaaacc aatattgaat
      781 ccggttatcc tgtacagctc ttggacaacc gatggaattg tgtcatgtac actcatcctc
      841 tttttgacag ggaagcctat atcttgcatt tttgctcttt tagaaatatg tcttatattt
      901 ttgctgaaaa atttctcgaa aaagtgagac aacagggtat taaaaataat tcgtttgtac
      961 aatactccat acttcatcct cattattctt acttgccatt tgaaaatgct atttatcgtt
     1021 acaagccaaa agattacatc cggatggcaa aggaaatcag gcaaacggct aaactattaa
     1081 aagaaaatct tgactttgac gactatatac cttcagcagg catttatcgt tatgccggga
     1141 aatgttttaa aaataaatgt tacttttggg gcagcttgtt aataacttgc tataaacttt
     1201 acagagtaaa attcgataaa aaattcaagt atgttgaaaa cacctgtgcg gctgaataat
     1261 aagggaaccg gttatgtaaa agatatcaat aatataccta tgacaggaga acgtgatgtt
     1321 catcttgacg ttctaaggat attggctgca tttttcgtaa tcatggtaca tgtagctggg
     1381 acaggattgt taaagggaag agattatgaa ttgggcagtt tgaattggta tatatgtatg
     1441 atctttagtt cattagctcg ttggagtgtt ccagttttcg tgatgattag tggtagttgt
     1501 ttccttccgc caactaaaac aataacttta aaaaaacttt ttggtaaata catactgaga
     1561 gtatttgttg cattagttgt ttggtcaagt ttctatgctt atttttttca tggaaaatat
     1621 attccacttg ggctgaatag tggtaatcat ctctggtatt tgacaatgat tattggaatt
     1681 tatcttgctt taccaatttt aaaagttata ccaacagttc tattgaaata ttttacatta
     1741 atctggttta tatttcttac aattgatttt atcaacaaat tgtctggtaa tgct
//
LOCUS       DAOTKS010000348         1769 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_54096_length_1769_cov_3.734539, whole genome shotgun sequence.
ACCESSION   DAOTKS010000348 DAOTKS010000000
VERSION     DAOTKS010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54096_length_1769_cov_3.734539"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="P5236_06385"
     CDS             complement(<1..228)
                     /locus_tag="P5236_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU64026.1"
                     /translation="MDKLRKQIKESFFNPIYHFFPLIVFLIVDNVAGRNWAWSIAIPL
                     FLILTIYLFIAYNQLFVWYFFSAFIFLAVSLS"
     gene            complement(309..914)
                     /locus_tag="P5236_06390"
     CDS             complement(309..914)
                     /locus_tag="P5236_06390"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HRU64027.1"
                     /translation="MFSGIIEEAAPVVALKKEEGNLHITMSCSFVNELTIDQSIAHNG
                     VCLTVVSKTDDSYTVTAIQETLDRTNLGELVCGNEVNLERSLLLTERLDGHIVQGHVD
                     TTAVCTAIDEVEGSWYFTFEYQFDPEMAKRGYLTVDKGSVAVNGVSLTVCNPTDNSFQ
                     VAIIPYTYEHTNFHQIKVGTTVNIEFDIIGKYISRLMKYNV"
     gene            complement(927..>1769)
                     /locus_tag="P5236_06395"
     CDS             complement(927..>1769)
                     /locus_tag="P5236_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5913 domain-containing protein"
                     /protein_id="HRU64028.1"
                     /translation="QKPSAEWLNIVATSKAKAKIRQALKEIQYKDAVIGRETLLRRFK
                     NWKIELGEGKISRLAKKKGYKTVSDFYQEIAQEKLDLLALRDTYLEMEKHEAQQNVTE
                     TRSAENFIKEPPVIETSIKEKEDILIIDQNLKGIDYTLAKCCQPIYGDEIFGFVSVGG
                     GIKIHRVDCPNAPELYSKFGYRIVKAQWSGQSGGTQYPITLHVIGKDDIGIVTNITSL
                     IDKEKNIVLRSISIDSKAGLFIGNLTIMVNDAHDLDGIMKKISMIKGIVNVSRIKTIN
                     KYNS"
BASE COUNT          548 a          344 c          274 g          603 t
ORIGIN      
        1 actcagactt acggcaagaa aaataaaagc agaaaaaaaa taccagacaa aaagctggtt
       61 ataagcaata aaaagataaa ttgtaagtat aaggaataaa gggatagcaa ttgaccatgc
      121 ccaatttctt cccgccacat tatctactat caagaaaacg attagcggga agaaatgata
      181 aattggatta aagaaagatt ctttgatttg ttttcttaat ttatccatag gtagtcattc
      241 aatcttctac attttactac ctataacaat cattgcaagc ttttggtttg ataacaactg
      301 tgagaatctc aaacattgta cttcatcaat cggctgatat atttgccaat aatgtcaaat
      361 tcaatgttaa ccgtagtacc tacttttatt tgatggaagt tagtatgttc ataagtataa
      421 ggaatgatgg ctacctgaaa agagttatcg gttggattgc aaacagtaag acttacgcca
      481 ttcacagcaa ctgaaccttt atctacagta agataaccgc gtttcgccat ttcgggatca
      541 aattggtatt cgaacgtaaa ataccagctt ccttctactt catcaatagc agtacaaaca
      601 gcagtagtat ccacgtgtcc ctgcacaata tgtccgtcca gtcgttccgt taaaagtaaa
      661 ctgcgttcca gatttacttc gtttccacaa accagttctc ccagatttgt tcgatcaagt
      721 gtttcctgaa tggcagttac tgtataacta tcatcggttt ttgaaaccac tgtcaagcaa
      781 acgccattgt gagcaatact ttggtcaata gtcaattcat ttacaaaaga acagctcatg
      841 gtaatgtgca gatttccttc ttcttttttt aatgccacta ctggagcagc ttcttcaatt
      901 attcctgaaa acatttctta aattttttat gagttatact tattaatggt ttttattcgt
      961 gaaacattta caattccttt tatcatgctg atttttttca taatgccatc taaatcatga
     1021 gcatcattaa ccataatagt caggtttcca ataaaaagtc ctgcttttga atcaatggaa
     1081 atagagcgta acacaatgtt tttttccttg tcaattaaag aagtaatgtt ggttacgatg
     1141 ccaatatcat ctttcccaat aacgtgtaaa gtaatgggat attgagtgcc acctgattgt
     1201 cctgaccatt gagcttttac aattctgtaa ccaaattttg aatacaattc aggtgcattg
     1261 ggacaatcta ctctgtgaat cttgatgcca cccccaacag acacaaatcc aaaaatttca
     1321 tctccataaa ttggttgaca acattttgcc agcgtataat caattccttt taaattttga
     1381 tcaataatta aaatatcttc tttttctttg atagaggttt caataacggg cggctctttt
     1441 atgaaattct cagcacttcg tgtttccgtt acattttgct gagcttcatg cttttccatc
     1501 tccaaatagg tatcccgaag agccaaaaga tcaagttttt cctgtgcaat ttcctgataa
     1561 aaatcactta ctgtcttata tccttttttc tttgccagac gagaaatttt accttcaccc
     1621 agctcaattt tccaattttt gaaacgtcgc aaaagtgttt ctcgtccgat aactgcatct
     1681 ttgtattgaa tttcttttaa tgcttgacga atttttgctt tggcttttga ggtagcaacg
     1741 atatttaacc attcggcact cggcttttg
//
LOCUS       DAOTKS010000349         1763 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_54368_length_1763_cov_3.930328, whole genome shotgun sequence.
ACCESSION   DAOTKS010000349 DAOTKS010000000
VERSION     DAOTKS010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54368_length_1763_cov_3.930328"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="P5236_06400"
     CDS             <1..234
                     /locus_tag="P5236_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRU64029.1"
                     /translation="GSLEFNQKLSVERAQSVANFLKSKGVPANQIKDVIGKNYSEPVA
                     DNSTPEGRAANRRVEVYMYASEQMIKEAEQQAK"
     gene            complement(416..940)
                     /locus_tag="P5236_06405"
     CDS             complement(416..940)
                     /locus_tag="P5236_06405"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRU64030.1"
                     /translation="MKNTRVLFSVVFSLCFVIACKKMPPQLPANKNVDDVQNTMSMME
                     VNHQLALREDSLLQVYVANLKDKSFKKTETGLWYKIEKTKQGKKIKNNDVCQFSYELF
                     SLDDKLLEKNEQEIIVIGKKQTIKGLEEGLQLLRKGDNAIFIIPWYLAYGMKGKEPLI
                     APYVSVIYRVKVFE"
     gene            complement(945..>1763)
                     /locus_tag="P5236_06410"
     CDS             complement(945..>1763)
                     /locus_tag="P5236_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HRU64031.1"
                     /translation="MNIFNSVFVDCTASDQIADLYQQLLDHNISVVAANKIAASSSYD
                     KYIRLKETALRKGIKFLFETNVGAGLPIISTINNLINSGDRIFKIEAVLSGTLNFIFN
                     TISEEIPLSKAIQLAKEKGFSEPDPRIDLSGKDVVRKLVILAREAGYRVEQEEVVKKL
                     FIPDKFFKGTLEEFWESISELDEEFEQKRQILASQGKRWKFVASMDHDYCEVGLQEVD
                     QHHPFYDLQGSNNIILLTTERYNEFPMMIKGYGAGASVTAAGVFSDIISIANIR"
BASE COUNT          592 a          332 c          256 g          583 t
ORIGIN      
        1 ggttctttag agtttaatca aaaattatct gtagaaagag cacaatcagt agctaatttt
       61 ctaaaatcaa aaggagttcc tgctaatcaa attaaagatg tgatagggaa aaattattca
      121 gaacctgtgg ctgataattc tacccctgaa ggaagagctg ccaatcgtcg agtagaagtt
      181 tatatgtatg ccagcgagca aatgattaaa gaggcagaac aacaagcaaa ataactattt
      241 tagagaaata tttaaagaag agacgtgcta ttttgggagt ctcttcttta gtattgttta
      301 gaaaacaaaa aaggaagaat taaccttttc aaaaaataat taaatttatc ccagaattgt
      361 cattaaaaat aaacagaaac tactatttcc tttaagttcg ttcaaattgg ctatattatt
      421 caaacacttt taccctgtaa attactgaaa cataaggagc tattaatggt tctttccctt
      481 tcatcccgta agccagatac catggaataa tgaaaatggc attatcgcct ttgcgtaaaa
      541 gttgcaatcc ttcttcaagt cctttgatag tctgtttttt tcctataaca ataatttctt
      601 gttcattttt ctcaagcaac ttgtcatcca atgaaaaaag ttcataagaa aattgacaaa
      661 catcattatt ttttattttc tttccttgct tagttttttc aattttatac cacaatccag
      721 tttcagtctt cttaaaactt ttgtctttca agttcgccac atacacttgc aataagctgt
      781 cttctcgaag tgctaattga tggtttactt ccatcattga catagtattc tggacgtcat
      841 caacgttttt attggctggt aattgtggtg gcattttttt gcaagcaata acaaaacaca
      901 aagaaaacac cacagaaaat aaaactcgag tgtttttcat gctctcaacg aatattagca
      961 atgctgataa tatcggaaaa tacccctgct gcagtaacac ttgctcccgc tccataacct
     1021 ttgatcatca taggaaattc attataacgt tctgtggtaa gtaaaatgat attattactt
     1081 ccttgcaaat cataaaaagg atgatgctga tctacttcct gaagtcccac ttcacaataa
     1141 tcatgatcca tagaagctac aaatttccaa cgttttccct gcgaagccaa aatctgtctt
     1201 ttttgttcaa attcttcatc cagttcggaa atactttccc aaaattcttc caatgttccc
     1261 ttaaaaaact tatcagggat aaataatttt tttactactt cttcctgttc cactcgatag
     1321 cctgcttcac gtgcaagaat aacaagtttt cgcaccacat ctttaccact taaatcaata
     1381 cgaggatcag gttctgaaaa acctttttct ttcgccaact gaatagcttt acttaaaggt
     1441 atttcttcac tgatggtatt aaaaataaaa ttcaacgttc cggaaagtac cgcttcaatt
     1501 ttaaaaatac ggtcgccgct atttatcaaa ttattaatgg tgctgataat aggtaaacca
     1561 gctcctacat tggtttcaaa aagaaattta attcctttac gaagagcagt ttctttcaac
     1621 cgaatgtatt tgtcgtacga agaagaggct gctattttat tagctgccac cacagaaata
     1681 ttatgatcga gtaattgttg atacaaatca gcaatttgat cactggcagt acaatccaca
     1741 aaaacagaat taaaaatgtt cat
//
LOCUS       DAOTKS010000350         1759 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_54551_length_1759_cov_4.528756, whole genome shotgun sequence.
ACCESSION   DAOTKS010000350 DAOTKS010000000
VERSION     DAOTKS010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54551_length_1759_cov_4.528756"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..1629)
                     /locus_tag="P5236_06415"
     CDS             complement(91..1629)
                     /locus_tag="P5236_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HRU64032.1"
                     /translation="MLVKTFGAAVQGINATIITIEVNVNQGIHFFLVGLPDNAVKESH
                     ERIASALDYIGYKMPRKQIVVNMAPADIRKEGSAYDLPLAIGMLAADEKIQANQLEQY
                     LIMGELSLDGSLNPIKGALPIAIKAREEKFKGFILPKQNAREAAVVNDLEVYGVENIK
                     QVVDFLNDNLNLEPTIVNTRQEFYENFNHFEIDFADVKGQENVKRALEVAAAGGHNII
                     MVGPPGSGKSMLAKRIPTILPPLTLHEALETTKIHSVAGKIGNNTSLMYERPFRSPHH
                     TISDVALVGGGTFPQPGEISLAHNGVLFLDELPEFKRSVLEVLRQPLEDRRITISRAK
                     FTIEYPASFMLIAAMNPCPCGYYNHPERECVCAPGVVQKYLNRISGPLLDRIDIHIEI
                     VPVPFEKLSDMKEGESSIKIRERVIKARQIQEIRFRDIEGVYCNAQMTTKLQRKFATP
                     DKSGLELLKTAMQRLSLSARAYDRILKVARTIADLDASEMILAHHIAEAINYRNLDRE
                     GWGS"
BASE COUNT          534 a          358 c          301 g          566 t
ORIGIN      
        1 gcatctgact gttaatcaga gggtccttgg ttcaagtcca agaggaagag caaagttgct
       61 ttcattggca acttttttat tttgtcaatt ttaacttccc caaccttctc gatccagatt
      121 tcgataatta attgcttcgg caatatgatg agcaagaatc atttccgaag catccaaatc
      181 tgcaatggtg cgagccactt tcaaaatccg atcataggct ctcgctgata aactcaagcg
      241 ttgcatagct gtttttaata attcaagccc agatttatca ggtgtggcaa attttcgctg
      301 caacttagta gtcatctgag cattacaata aactccctca atatctctaa atcttatttc
      361 ctgaatttga cgagctttaa taaccctttc acggattttg atactacttt cgccttcttt
      421 catatctgaa agtttttcaa aaggaacagg tacaatttca atatgaatat caatcctgtc
      481 cagtaaagga ccagaaatac gattcaggta tttttggacg acacccggcg cacaaacaca
      541 ttctcgttct ggatgattat aataaccgca agggcaggga ttcatagctg ctatcagcat
      601 aaaactggca ggatattcaa tggtaaattt tgcacgtgaa atcgtaattc tgcggtcttc
      661 aagaggctgt ctcaacactt caagaacaga acgcttaaat tcaggaagtt cgtcaagaaa
      721 aagtacccca ttgtgagcca gagaaatttc acctggctgc gggaaagtcc ctcctcctac
      781 taaggcgaca tctgaaatag tatgatgtgg acttctgaaa ggacgttcat acatcaaaga
      841 agtattattg ccaatttttc cagctacaga atgaattttt gttgtttcaa gggcttcgtg
      901 taatgttaat ggtggcaaaa tggttgggat tcgctttgcc agcatagatt taccagatcc
      961 aggaggacct accataataa tgttatgtcc acctgctgct gcaacttcta aggctctttt
     1021 tacattttcc tgacctttta catcagcaaa atcaatttca aaatgattga aattttcata
     1081 aaattcttgc cgtgtattga caatagtagg ttcaagattc aaattatcat ttagaaagtc
     1141 aactacctgc ttgatatttt ctaccccata aacttccagg tcgtttacta cggcagcttc
     1201 acgggcattc tgcttgggca gaataaatcc tttgaatttt tcttctcgtg ctttgatggc
     1261 aataggcaaa gctcctttaa taggattcaa actcccatcc agcgacaatt cacccataat
     1321 aagatattgt tctaattgat tggcttgaat tttttcatca gcagccagca taccaatagc
     1381 caatggaaga tcgtaagcag aaccttcttt gcgaatgtct gctggtgcca tattcaccac
     1441 tatctgcttt cggggcattt tatagccaat ataatccaga gcagaagcta tgcgttcatg
     1501 actttctttt actgcattat caggtaaacc taccaaaaag aaatggatac cttgatttac
     1561 atttacttca atagtgataa ttgtagcatt aatcccttga actgcagctc caaacgtttt
     1621 aactaacatt gataacgatt taaaattaaa aaattaagtc atttttaatt ctgacaagca
     1681 attgttttgc tatttttttt aaaagaatga tcaagtaata cttttactgt aaacaattgt
     1741 tcagtaaata atttccctt
//
LOCUS       DAOTKS010000351         1739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_55504_length_1739_cov_3.390736, whole genome shotgun sequence.
ACCESSION   DAOTKS010000351 DAOTKS010000000
VERSION     DAOTKS010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55504_length_1739_cov_3.390736"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1410
                     /gene="alr"
                     /locus_tag="P5236_06420"
     CDS             <1..1410
                     /gene="alr"
                     /locus_tag="P5236_06420"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRU64033.1"
                     /translation="QQAASKDLSRTLILSDILQSGHSSEELYKTVAFLVKSKNIQQMI
                     GIGSEISKYADEFNFEDKNFFETTEDFLKSSLIYTFQDKIILLKGSRKFQFEKILERL
                     ELVAYETTLEVNLNALIENFNYFRSKLLPETKIMSMVKAFAYGSGSEEVARVLQHHHC
                     DYLAVAVADEGAELREEGIRIPIVVMNPEKGSFRMILDYQLEPEIYSFRILESFLTAT
                     EKLGIVDYPIHIKIDSGMHRLGFDPSEVDKLISILKNQNQLKVRSVFSHLAASDEPAF
                     DNFTRQQVETFSSCADKFSAAFPHHILRHILNSAGIERFPEYQFDMVRLGIGHYGISS
                     LPNIKLRQVCSLKTIILQIKNVKKGETIGYGRKAMMKEDARIAVIPIGYADGFDRKLG
                     NGVGEVLVNGKTAKVVGNVCMDLTMIDVTHLDIKEGDVVEIFGEHLLISEISKKLNTI
                     PYEVLTRVSRRVKRVYIYE"
     gene            1441..>1739
                     /locus_tag="P5236_06425"
     CDS             1441..>1739
                     /locus_tag="P5236_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU64034.1"
                     /translation="MILALIQKIETCDNLLKSASYLAVQLKKKFGILLINNKKEELIE
                     KEKSYLNSKMNSLQLLSQPDEILYATSGKELTEMCEKIEASFLFIQLLDDSKKNIR"
BASE COUNT          606 a          238 c          310 g          585 t
ORIGIN      
        1 caacaagcag cttccaaaga tttatcgcga actcttattc tttctgatat tttacaaagt
       61 ggacattctt cagaagaatt atacaaaaca gtagcttttc tggtgaaaag taaaaatatc
      121 cagcaaatga tcggcattgg ttcagaaata tccaaatatg ctgacgaatt taattttgag
      181 gataaaaatt tctttgaaac gacggaagat tttttaaaat cttctttgat ttatactttt
      241 caagataaaa ttattcttct caaaggttcc cgtaaatttc aatttgaaaa aattctggaa
      301 aggttagaat tagtagctta tgaaacaacg ttggaagtaa atttgaatgc cttgattgaa
      361 aatttcaatt attttcggtc aaaactttta cctgaaacaa aaattatgag tatggtaaaa
      421 gcttttgctt atggaagcgg ttcagaggaa gtggcaagag ttttacagca tcatcattgt
      481 gattatcttg ctgtagctgt tgctgacgaa ggtgcagaat taagagaaga aggtattaga
      541 attcctatcg ttgtaatgaa tccagaaaaa ggaagtttca gaatgattct ggattatcaa
      601 ttagaacctg aaatttatag ttttcgtata ttggaatctt ttttaaccgc tacagagaaa
      661 ttaggtattg tggattatcc gattcatatt aaaattgaca gtggcatgca tcgattggga
      721 tttgatcctt ctgaagttga taaattaatt tctattctga agaatcagaa tcagctaaaa
      781 gttcgttcag ttttttctca tctggcagct tcggatgaac cagcatttga taattttaca
      841 agacaacaag tagaaacttt ttccagttgt gccgataagt tttcagcagc tttcccccat
      901 catatcttgc gtcatatttt gaattctgct ggaattgaac gtttcccaga atatcaattt
      961 gatatggtac gacttggaat tggacactat ggtattagtt ctttgccaaa tattaagcta
     1021 aggcaagttt gctcgttaaa aacaattata ttacaaatta aaaacgtcaa aaaaggagaa
     1081 actatcggtt atggaagaaa agctatgatg aaagaagatg ctcgaattgc agtgattccg
     1141 attggttatg ctgatggttt tgatcgtaaa ttgggaaatg gtgtaggtga agttttagtt
     1201 aatggtaaaa cagcaaaagt agttggaaat gtctgcatgg acttaactat gatcgatgtg
     1261 acccatcttg acataaaaga aggtgatgtt gtagaaattt ttggtgaaca tttacttatt
     1321 tcagaaattt ccaaaaaatt aaataccatt ccttacgaag tattaacgcg tgtttcacgt
     1381 cgggtaaaac gagtttatat ttacgaataa aaattgctaa actttttcct gtaaaaaaat
     1441 atgattttag ctttaattca aaagattgaa acgtgtgata atttattgaa gagtgcctct
     1501 tatcttgccg ttcaattaaa aaagaaattt ggtattttat tgataaacaa taagaaagaa
     1561 gaattaattg aaaaagaaaa atcttattta aattctaaaa tgaattcttt acaacttctt
     1621 tctcaacctg acgaaattct ttatgctact tctggaaaag agcttactga aatgtgcgaa
     1681 aaaatagaag catcatttct tttcatccaa ttgcttgacg attcaaaaaa aaatattcg
//
LOCUS       DAOTKS010000352         1725 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_56147_length_1725_cov_3.556287, whole genome shotgun sequence.
ACCESSION   DAOTKS010000352 DAOTKS010000000
VERSION     DAOTKS010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56147_length_1725_cov_3.556287"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1631
                     /locus_tag="P5236_06430"
     CDS             <1..1631
                     /locus_tag="P5236_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HRU64035.1"
                     /translation="LYEEEFGAVRCFEWSPDSKLLAFIKFDETEVPEFSYQLYADSTK
                     MIDGLSLYPTTVTYKYPKAGQNISKVSVCIFDERQKSIRTIELNTLEKNFYIPRIKWT
                     SSVDQLAIFTLNRNQNEMKMFLANPKSTLSKLILQEKDDNYIDYRNFNFYQFSSDNQY
                     FIGVNEKNGYRHVYQYQINGTLIKQLTAGNWDVTDVYGFDENTQTLYYQSAETSPLQR
                     DVYAITTKGKKTRLTDGKGVHQAIFNSNFTYFVDNASSLENANTLTVCNNKGNFIRIF
                     QNNDSITQEWEKLNLPKKEFFSFTTSENIKLNGWILKPSNFDTTKKYPLLLVQYSGPD
                     AQEVLDEWNVDWEYYLATQNYVVACVDGRGTGARGVKFRKCTYQQLGILETKDQIETA
                     KFLSSQHYIDKERIGIWGWSYGGFITLMAMSSPEKIFKTGIAVAPVTDWRLYNAAYAE
                     RYMRRPQENFKGYDLTSPLLHATHLNGNLLIIHGTADDNVHAQNTLLYAQHLEENNKK
                     FQMLLYTDKNHSILGKETRRHLYQQMSEFLFMNL"
BASE COUNT          641 a          249 c          282 g          553 t
ORIGIN      
        1 ggctttatga agaagaattt ggtgctgttc gctgtttcga atggagccct gatagcaaat
       61 tgttggcatt tataaaattt gatgaaaccg aagttcctga attttcttat caactttatg
      121 ccgactctac taaaatgata gatggactgt cgctttatcc gacaaccgta acttataaat
      181 atcctaaagc aggtcaaaat atctcaaaag tcagtgtttg tatttttgat gaaagacaaa
      241 aatcaataag aacaatcgaa ttaaatactt tagaaaaaaa cttttatata ccacgaatta
      301 aatggacttc ctccgtggat caattggcta tttttacctt aaatcgaaat caaaatgaaa
      361 tgaaaatgtt tttagccaat cctaaatcta ctttatcaaa attaattctt caggaaaaag
      421 acgataatta tattgattat agaaatttca atttttatca attttcttct gacaatcaat
      481 attttattgg tgtaaatgaa aaaaatggct accgacatgt ttatcaatat caaataaatg
      541 gaactttaat taaacaacta actgctggga attgggatgt tactgatgtt tatggttttg
      601 atgaaaatac ccagacactt tattatcaat ctgctgaaac gtcgccgctt caacgggatg
      661 tttatgctat tacaacaaaa ggaaaaaaaa caagattaac agatggaaaa ggagttcatc
      721 aagctatttt taatagtaat tttacttatt ttgttgacaa tgcttcttct cttgaaaatg
      781 caaatacttt aacagtttgt aataataaag gaaatttcat acgtattttt caaaataatg
      841 actccataac ccaagaatgg gaaaaactaa atttaccaaa aaaagaattt ttcagtttta
      901 ctacttcaga aaatataaaa ctaaatggct ggatattaaa accttccaat tttgatacaa
      961 caaaaaaata tcctttgctg cttgttcaat atagtggtcc tgatgctcaa gaagttttag
     1021 atgaatggaa tgtagattgg gaatattatt tagccactca aaattacgta gtagcttgcg
     1081 tggatggacg aggaactgga gctcgaggtg tgaaatttcg caaatgtact tatcaacaat
     1141 taggaatttt agagacaaaa gatcaaatcg agacagcaaa gtttttgagt tctcaacatt
     1201 acattgacaa ggaacgaata ggaatttggg gatggagtta tggaggtttc attacgctta
     1261 tggcaatgag tagtccagaa aaaatattta aaaccggtat tgctgtggct cctgttacag
     1321 attggagatt atacaacgct gcttatgcag aacgatatat gcgacgacca caagaaaact
     1381 tcaagggata tgatttgacc tctcccttat tgcatgccac tcatttgaat ggtaatttat
     1441 taattattca tggcacagca gatgataatg tacacgcaca aaatacttta ttatatgccc
     1501 aacatttaga agaaaacaac aaaaaattcc agatgttgct atatactgat aaaaatcata
     1561 gtattttggg aaaagaaacc agaagacatt tatatcaaca aatgagcgaa tttctattta
     1621 tgaatttgta aacgaattat aaatcaaaac aaaaatagtg attgaaacaa aaaatatgtt
     1681 gtaaaacatt gtttatctga tgtatttttt gatgttcttt ttaag
//
LOCUS       DAOTKS010000353         1721 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_56345_length_1721_cov_3.087635, whole genome shotgun sequence.
ACCESSION   DAOTKS010000353 DAOTKS010000000
VERSION     DAOTKS010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56345_length_1721_cov_3.087635"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            546..>1721
                     /locus_tag="P5236_06435"
     CDS             546..>1721
                     /locus_tag="P5236_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRU64036.1"
                     /translation="MQNKFKFVSAEEAAAYVQHKDVVGFSGFTPAGCPKAVPLAIAKR
                     AETFYAEGKEFKIGMYTGASTGVSVDGALARSDAVLFRTPYQSNKDMRNAINAGKAPY
                     FDMHLSTLAQELRFGVLPKPKFAILEVCDWNEEGELVLTAAVGISPTIADLADHIILE
                     WNHYHPKELRGFHDIYQPQDPPYRREIPIYKPSDRIGSEILKVDPSKILCIVETNLPD
                     EVLDFAPLDEVTKQIGLNVATFLVDEIRSGRMPHGFLPIQSGVGNIANALLFSLGKNE
                     EVPPFEMYTEVIQDAVIELMIEGNIKFASGCSLTVTNDVLQKIYQNLDFFRDKLVLRP
                     QEISNNPEIVRRLGLITINTALEADIFGNVNSTHVLGTKMMNGIGGSGDFTRNSFISI
                     "
BASE COUNT          540 a          272 c          325 g          584 t
ORIGIN      
        1 actaataact tcttttttcc ctgaaggacg cggaatctgt gtcagactgt aaaagttttg
       61 ccacaattgc tgcggttgaa gctgacgaat atcattgttg ttcataatat gtttattatt
      121 agaattttgt aaattctgaa tatttattcc aatttttcaa actacaaata tgctcaaatt
      181 tttcagataa atgaactgtt gaggagtttt tttacaaata ttgagaatga attttcaaaa
      241 cgggaagtaa gaaaagtaca attgaaatca attttaagtc gcttcctaga acggctctac
      301 aagaaattac cgataaaatt ccagagacat ttctttattt aaccttttct aataattcct
      361 gtattttagc agcgttatag ttataatttt tgatatttat cttctaaata atttttcacc
      421 aataatttat tagcaaaata aggattactc ctgaaaaatt tccttaatca gttttgagta
      481 tcttgaagga ttgggaaata tttgataatt ttgtgcagaa tttttaagaa taaactaact
      541 tttatatgca aaataaattt aagtttgtta gtgcagaaga agcagcagct tacgttcaac
      601 ataaagacgt ggtaggtttt agcggtttta ctccggcagg ttgtccgaaa gcggttcctt
      661 tagcaatagc caaacgtgca gaaacttttt atgctgaagg aaaagaattt aaaatcggaa
      721 tgtacactgg agcctcaact ggagtttcag tagatggtgc tttagctcga tctgatgcgg
      781 tattatttcg aactccttac cagtcgaaca aagacatgcg taatgctatc aatgcaggaa
      841 aagctcccta ttttgacatg catttatcta cactggctca ggaactgcgt tttggagttt
      901 taccgaaacc aaaattcgct attctggaag tatgtgactg gaatgaagaa ggtgaattgg
      961 tattaacagc agcggtagga atttcgccaa ctattgccga tttggccgat catattattt
     1021 tggaatggaa ccattatcat cccaaagaat tgagaggttt tcacgacatt tatcagccac
     1081 aggatccacc ttaccgcaga gaaattccca tttataaacc ctctgacaga attggctcgg
     1141 aaattttgaa agtcgatcct tctaaaattc tttgtattgt agagactaat ttaccagatg
     1201 aggtattgga ttttgctcca ttggatgaag taactaaaca aattggtttg aatgtggcta
     1261 cttttttggt tgatgaaatt cgttcaggaa gaatgccaca tggtttttta cctattcagt
     1321 caggtgttgg taacattgcc aatgctttgc tgttttcttt aggcaaaaat gaagaagttc
     1381 caccttttga aatgtatact gaagtgattc aagatgccgt gattgaactg atgatagaag
     1441 gaaacattaa atttgccagt ggttgctcgc tgacggttac caatgatgta ttacagaaaa
     1501 tatatcaaaa tcttgatttt ttccgcgata aattagtgct tcgtcctcaa gaaatttcta
     1561 ataatcctga aatagttcgc cgtttgggtt tgattactat caatacagca ctggaagctg
     1621 atatttttgg gaatgtaaat agcactcatg tacttggaac caaaatgatg aatggtattg
     1681 gaggttcagg cgattttaca cgtaattcat ttatttccat t
//
LOCUS       DAOTKS010000354         1718 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_56473_length_1718_cov_5.897174, whole genome shotgun sequence.
ACCESSION   DAOTKS010000354 DAOTKS010000000
VERSION     DAOTKS010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56473_length_1718_cov_5.897174"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..80)
                     /locus_tag="P5236_06440"
     tRNA            complement(7..80)
                     /locus_tag="P5236_06440"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44..46),aa:Met,seq:cat)
     gene            complement(268..1716)
                     /locus_tag="P5236_06445"
     CDS             complement(268..1716)
                     /locus_tag="P5236_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRU64037.1"
                     /translation="MQEWNNESIKEYLQHPIFKIISETADEQQEECYVIGGFVRDIFL
                     KRSSKDIDIVVIGSGIELAKRVVEKLDKKAKLTVFKNFGTAQVKTNDLEIEFVGARKE
                     SYRSDSRKPIVENGTLEDDQKRRDFTINTLALCLNKNRFGELVDPFNGYRDLQNSVLR
                     TPLDPDITFSDDPLRMLRCIRFATELNFYVEVNTFEAIRRNKKRIEIISKERITDELN
                     KIILSKKPSIGFILLDQSGLLELIFPELFALKGVETKDGIGHKDIFYHTLAVLDNLSF
                     QTDSLWLRWAALLHDIGKPLTKCFDKQTGWTFYNHNVVGSKMVADIFRKMRLPVNEKM
                     KYVQKLVFLHMRPIVLSEEEVTDSAVRRLLFEAGNDIDDLMMLCEADITSKNPEKVKQ
                     YRNNFQLVRKKLKEIEEKDRIRNFQPPIDGNEIMKTFNLPACATVGEIKQRIKDAILD
                     GVIPNEYEAAREYMMKIGKEMGLIPSSISNYE"
BASE COUNT          539 a          321 c          242 g          616 t
ORIGIN      
        1 ttttagtagc gggaacgaga gttgaactcg tgacctccgg gttatgaatc cgacgctcta
       61 acctactgag ctatcccgcc ttctgttttt gcaatgcaaa gataagcttt ttgaaaaaat
      121 tagacaaaac tttgatttga ataagatgag attatttgta aaaaaccagg taataaaaat
      181 gaaattttaa aagatttatt tccttttggg gggaatttta gaaatgaacc tttttgaaaa
      241 tagtaaataa agataaagca ttttatttta ttcataattg ctaattgatg atggaattaa
      301 tcccatttct ttaccgattt tcatcatata ttctcgagct gcttcatatt cattgggaat
      361 aacgccatcc aaaatggcat ctttaattcg ctgctttatt tctcctaccg tagcacaggc
      421 tggcaaattg aatgttttca taatttcatt tccatcaata ggtggctgaa aattacgaat
      481 tcgatctttt tcttcaattt ctttcagttt ttttcgtacc aactgaaaat tatttctata
      541 ttgcttaact ttttccgggt tttttgaagt tatatccgct tcacaaagca tcatcaaatc
      601 atcaatatca ttacctgctt caaaaagtaa tctcctgacg gctgaatcag taacttcttc
      661 ttcacttaac acaataggac gcatgtgtaa aaaaactaat ttttgaacat atttcatttt
      721 ttcattgacg ggtaaccgca tttttctgaa aatatcggct accatttttg aacctacaac
      781 attatgattg taaaaagtcc agccagtctg tttgtcaaaa cattttgtta gcggttttcc
      841 aatatcatgc aaaagagctg cccatcgcag ccacagcgaa tctgtctgaa aacttaaatt
      901 atctaatacg gcaagtgtat gataaaaaat atccttatgt ccgattccat ctttggtttc
      961 cactcctttt agagcaaaaa gttcaggaaa aataagttca agcagtcctg actgatcgag
     1021 caaaataaaa ccaatggatg gtttttttga taaaataatt ttgtttaatt catcggtaat
     1081 tctttctttt gaaataattt ctattcgttt tttatttcgg cgaatcgctt caaaagtatt
     1141 cacttccaca taaaaattca gctctgttgc aaaccgaata catcttaaca ttcgtaaagg
     1201 atcatcagaa aaagtaatat caggatccaa tggagttctt agcactgaat tctgcaaatc
     1261 tctatatccg ttaaatggat ccactaattc accaaaacga tttttattca agcataatgc
     1321 caacgtattt atggtaaagt cacgacgttt ttgatcgtct tccagcgttc cattttctac
     1381 aatgggtttt cgtgaatctg agcgatacga ttctttacga gctcctacaa attcaatttc
     1441 taaatcgttt gtttttactt gtgctgttcc aaaattttta aaaacagtca attttgcttt
     1501 tttatccaat ttttcaacca ctcgttttgc cagttcaatg ccacttccaa ttaccacaat
     1561 atcaatatct ttcgacgatc ttttcaaaaa aatatcgcgt acaaaaccac caataacata
     1621 acattcttct tgttgttcat cagcagtttc ggaaataatc ttaaatattg gatgctgtaa
     1681 atattctttt atagattcat tattccattc ttgcaaat
//
LOCUS       DAOTKS010000355         1711 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_56792_length_1711_cov_5.006039, whole genome shotgun sequence.
ACCESSION   DAOTKS010000355 DAOTKS010000000
VERSION     DAOTKS010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56792_length_1711_cov_5.006039"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..931)
                     /locus_tag="P5236_06450"
                     /pseudo
     CDS             complement(1..931)
                     /locus_tag="P5236_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005825039.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            complement(959..1540)
                     /locus_tag="P5236_06455"
     CDS             complement(959..1540)
                     /locus_tag="P5236_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007757729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRU64038.1"
                     /translation="MKKNVVIILIIVAALVLWGVSMYNSLVSSEEAVSSQWSNVENQY
                     QRRADLIPNLVATVKGYAAHERGTLEEVVQARAKATQITVDANSLTPEKLQQFQQAQG
                     ELSAALGRLLMIAENYPDLKANQNFIELQAQLEGTENRIAVERNRFNEMARNYNASIR
                     RFPKSIIAALFGFEKKPYFEAEAGTEKAPKVEF"
BASE COUNT          524 a          381 c          257 g          549 t
ORIGIN      
        1 atagcctcaa tgttatcttc cagctgttta acagaacttg cacctacaat aactgaattc
       61 acttcaggta atctcaaaca ccacgccaaa gccatttgag caagtgtttg tcctcttttt
      121 tgagccattg cattcagctc tttaacttta gcaatcactt cgggtgtaat ttgacttttt
      181 tgcaaaaaac catactgact agcagctctg gatttttctg gaatgccatt caaatattta
      241 tcggttaaaa ttccctgagc taacggagaa aaggcaatga atccaacacc attttctttt
      301 aaaaccggga acatattaaa ttcaacattt cgactaaaaa tattatacct gtcttgataa
      361 atcaagcaag gcgtcccatt ggcacgtaaa atttgaaaag cttcctttga tattttgaaa
      421 tacccacata aagagctttg ccctgtttga ccatatcact caaagctccc attgtttctt
      481 caagaggcgt attcggatcg taacgatgcg aatagaaaat atcaacgtat tcaagtccca
      541 ttcgcttcag actttgatga agacttgcaa tcatatattt tctggagcca ccgtcaccat
      601 aaggacctgc ccacatttca tgaccagctt ttgtactaat aaccatctca tcgcgatggg
      661 aagaaaaatt ttctttcaag attttcccga aatttttctc agcactgcca ggaggataac
      721 cataattatt tgccaaatcg aaatgagtca ttccatgatc aaaagcaaat tttatcattt
      781 tttcagcttc ctgaaaatcg tccacatccc caaaattgtg ccacaaaccc aaagaaatcg
      841 caggtaactg tagtccactt tttccacaaa accgataacc tactgaatga atatatcgct
      901 caggattggg agtataaaga ggatttgtca tagttttgtt ttttctttta aattgatttt
      961 aaaattcaac tttgggggct ttttctgttc cagcttctgc ttcaaaataa ggttttttct
     1021 caaaaccaaa taaagcagca ataattgatt ttgggaaacg ccggatagaa gcattgtaat
     1081 ttcgagccat ttcattaaaa cgatttcgct ccaccgcaat tctattttcc gttccttcca
     1141 actgtgcttg caattcaatg aagttctgat tggcttttaa atctggataa ttctcagcaa
     1201 tcatcaataa tctacccaaa gctgcactta attctccttg tgcttgttga aactgctgaa
     1261 gtttttcagg cgttaatgaa ttagcatcta cagtaatttg agttgcttta gcacgagctt
     1321 gtactacttc ctctaatgtt cctctttcgt gagcagcata acctttaact gttgcaacca
     1381 gattaggaat taaatctgct cgacgctggt actgattttc cacattcgac cattgacttg
     1441 aaacagcttc ttcagaagag actaaactgt tgtacataga aacaccccat aagacgagtg
     1501 ctgctacaat aattaaaata ataacaacat tctttttcat aaaataattt ttttatttgt
     1561 ttttgatcaa tttctaatta ttttttagcc aaatttacca gccgctggta gcaccaccgc
     1621 cacctgacat acctccgccg aaacttcctc caccaaaacc tccactacca ctaaaaattc
     1681 ctccgggaat agaacccgca ataatggtat t
//
LOCUS       DAOTKS010000356         1700 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_57304_length_1700_cov_3.584195, whole genome shotgun sequence.
ACCESSION   DAOTKS010000356 DAOTKS010000000
VERSION     DAOTKS010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57304_length_1700_cov_3.584195"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(300..941)
                     /gene="nth"
                     /locus_tag="P5236_06460"
     CDS             complement(300..941)
                     /gene="nth"
                     /locus_tag="P5236_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRU64039.1"
                     /translation="MTTEERYQHILKWFSENMPNPQTELHYNNPFELLVAVILSAQCT
                     DKRVNMVTPQLFAAYPTPEAMAKASPEDILKYIQSISFPNSKAQNLVAMAQKLVSDFG
                     GKVPDDVEQLQTLPGVGRKTANIIAAVIYNKPVMPVDTHVFRVAERLGLTTNSKNPLE
                     TEQELVKHIPPELLPKAHHWFILHGRYVCVARKPKCEQCGLKEWCRYYSEHSK"
     gene            1177..>1700
                     /locus_tag="P5236_06465"
     CDS             1177..>1700
                     /locus_tag="P5236_06465"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HRU64040.1"
                     /translation="MAPKKPIKNSSKSTSKTHSSSPKKENKVSFWKKINFFINDERTH
                     FILGLLFLLIMLYFLLAFVSYFFTGYADQSKIDLSWSELHQMRNSIQNWTSVLGAITA
                     ETLINRWFGVSSFFILFFGIVVALRLMKAVKLSLWKTFFHCAFWLIWLSVTLGFVFSP
                     FYNNVLFFSLGGQHG"
BASE COUNT          540 a          252 c          274 g          634 t
ORIGIN      
        1 aataatattt tattaaatgc agaaaatcac ggtcattatt ttgtttgtaa aaaaacttca
       61 tctgccttta atgagaaaaa attcttcttt tattaaaaat acgacctcaa tttattcaac
      121 attttatcaa tatattatat tgatattcaa ataattataa agaattttta taacaacctt
      181 tattagaaag ttttcactta atgacaaatt tgagattatt gattttgaca atcaaagatt
      241 tgtttatacc aaatcctacc ataattaatg ttgagcaaga gaagaatata ttcacatttt
      301 tatttggaat gttcggaata atatcggcac cattctttca aaccacattg ttcacattta
      361 ggtttgcgag ccacacaaac ataacgtcca tgtaaaataa accagtgatg agcttttgga
      421 agaagttcgg gaggaatatg ctttaccagt tcttgctcag tttccaaagg attttttgaa
      481 tttgttgtca atccaagacg ttcagcgact ctaaatacat gggtatctac tggcatcaca
      541 ggtttattgt aaatcacagc agcaataatg ttagctgttt tccgtccgac acctggtaaa
      601 gtttgcaatt gttccacatc atcggggact tttcctccaa aatctgaaac taatttttga
      661 gccattgcca ccaggttttg tgctttgctg ttaggaaaag aaatgctttg aatatatttt
      721 aaaatatctt cgggagaagc ttttgccatg gcttcggggg tagggtaagc agcaaatagt
      781 tgaggagtta ccatgttaac tcgtttgtct gtacattggg cagaaagaat tacagcaacc
      841 aacaattcaa aaggattgtt gtaatgtaat tctgtttgag gattgggcat gttttcagaa
      901 aaccatttta aaatatgctg ataacgttct tctgtggtca tttttatttg aagttttaag
      961 aaaaataaga attattcaaa tattgcttgt tgttcttgaa aatatcaaaa aaaaatcatt
     1021 cttttgcgaa atattttcaa ccttgcaaat ttaaaccaaa aaatggtttt tcatctctct
     1081 ttttgttatt tttgcaaaag aaatgaataa atttagaaaa gaaaaaagaa agttattatt
     1141 ttttgaataa atccttaaag tttaatttat tattaaatgg cacctaaaaa accgataaaa
     1201 aatagcagca aatccacctc aaaaactcat tcttcctctc caaaaaaaga gaataaagta
     1261 agtttttgga aaaaaataaa tttttttatt aatgatgaac gaactcattt tattttgggg
     1321 ttactttttt tgttgatcat gctttatttt ttattggctt ttgtttctta cttttttacc
     1381 ggatatgctg atcaaagtaa aattgacctt tcatggagcg aattacatca gatgcggaat
     1441 tctattcaaa actggacttc tgtgctcggg gcaattacgg ctgaaactct tattaatcga
     1501 tggtttggcg tttcttcctt ttttattctt ttttttggaa tagtggtagc ccttcgttta
     1561 atgaaggcag ttaaactatc tctgtggaaa actttttttc attgtgcttt ctggttgatc
     1621 tggctatcgg ttactttagg gtttgttttt tcaccttttt ataacaatgt actttttttc
     1681 tcattgggtg gtcagcatgg
//
LOCUS       DAOTKS010000357         1689 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_57804_length_1689_cov_4.634027, whole genome shotgun sequence.
ACCESSION   DAOTKS010000357 DAOTKS010000000
VERSION     DAOTKS010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57804_length_1689_cov_4.634027"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1689
                     /gene="priA"
                     /locus_tag="P5236_06470"
     CDS             <1..>1689
                     /gene="priA"
                     /locus_tag="P5236_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRU64041.1"
                     /translation="SDKKEITISQLIKKTGINNILPLLNSLLEKNAIEIKEALSEKIR
                     PKTEIFVRLNPSVQNEEQLHHIFDSLARAKKQLDLLMTYVELSKCLSPQPVEVSKKIL
                     LEKSGVSATTLDALVKRKILETYTKEIDRLNHSALPVNDLYPLNSFQQKALLEIEEQF
                     LRKNVVLLHGVTSSGKTELYLHLIQKTLNEGKQVLYLVPEIALTTQLTSRLKRVFGNK
                     LGVYHSRFSDAERVEIWNNVLNNKGYEVIIGARSSIFLPFRQLGLIIVDEEQETSYKQ
                     FDPAPRYHARNAAIVLASLHEAKTLLGTATPAIETYYNAQIGKYGLVELNQRYLELSM
                     PEILVVDLKEAYRKRQIEGHFTPLLLEKMKNALENREQIILFQNRRGYARYLECKMCA
                     YVPKCKNCDVSLTFHKTSNTLICHYCGYSEPISTVCPACGTPHGLSNKGFGTEKIEDE
                     IRKFFPEARIARMDLDTTKSRKSYEKIIADFEQYKIDILIGTQMVTKGLDFDRVSLVG
                     ILNADNLFHFPDFRAYERAFQLLAQVSGRAGRKNKQGTVILQTFEPQHPIIQQV"
BASE COUNT          573 a          296 c          309 g          511 t
ORIGIN      
        1 ttctgataaa aaagaaatta ctatttctca actcataaaa aaaacaggta tcaataatat
       61 tcttccttta ctcaattcat tattagaaaa aaatgctatt gaaataaaag aagcattgtc
      121 cgaaaaaatt cgtcccaaaa ctgaaatttt tgtccgattg aatccatcag ttcaaaatga
      181 agaacagttg catcatattt ttgattcgct tgcccgtgca aagaagcaat tggatttatt
      241 aatgacttat gtcgaacttt caaaatgttt gtcaccgcag ccagtcgagg tttcaaaaaa
      301 aatattactg gaaaaatcag gtgtctctgc tactactttg gatgcattgg tcaaacgaaa
      361 gatattagaa acctacacca aagaaattga ccgattgaat cacagtgctt tgccagtgaa
      421 tgatttgtat ccattgaata gttttcagca aaaagctttg ttggaaattg aagagcaatt
      481 tcttcgaaaa aatgtagttt tacttcatgg agtaacttcc agcggcaaaa cggaacttta
      541 tcttcattta atccaaaaaa cactcaacga aggcaaacaa gtgctttatc ttgttcctga
      601 aatagctttg actactcaac ttacttctcg attgaagcgt gtgtttggaa ataaactggg
      661 agtttatcat tccagatttt ctgatgctga acgtgttgaa atttggaata atgttttgaa
      721 taataaagga tatgaagtta ttattggggc tcgttcttct atttttcttc ctttccgaca
      781 attaggatta atcattgtgg acgaagagca ggaaaccagc tacaaacaat ttgatccagc
      841 tcctcgctat catgcacgca atgcagctat tgttctggct tccttgcacg aggcaaaaac
      901 tcttttagga acagctactc ctgccatcga aacttattat aatgctcaaa ttggaaaata
      961 tggactggta gagctgaatc aacgttatct tgaactttcc atgcctgaaa ttttagttgt
     1021 cgatttgaag gaagcatacc gcaagaggca aatagaagga cactttacac cattgttgct
     1081 ggaaaaaatg aaaaacgccc ttgaaaatag ggaacaaatc atattattcc aaaatcgacg
     1141 tggttatgca cgttatcttg aatgtaaaat gtgtgcttat gttccaaaat gtaaaaactg
     1201 tgatgtaagc cttacctttc ataaaacttc aaacacgcta atttgtcatt attgtggata
     1261 cagtgagcct atttcgacag tttgtccagc ttgcggaacg ccacatggac tttccaacaa
     1321 aggttttggt acagaaaaaa ttgaagatga aattagaaaa tttttccctg aagcacgaat
     1381 tgcccggatg gacttggata cgaccaaaag tagaaaatcc tatgaaaaaa ttattgctga
     1441 ctttgaacag tataaaattg acatacttat tggtactcaa atggttacaa agggtttgga
     1501 tttcgacaga gtaagtttag taggaattct gaatgcggat aatctttttc actttccaga
     1561 tttcagagct tatgaaagag ctttccagct actggcacag gtgagtggta gagcaggaag
     1621 aaaaaacaaa caaggcaccg tcatattgca gacttttgaa cctcaacatc caattattca
     1681 gcaagtgat
//
LOCUS       DAOTKS010000358         1676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_58433_length_1676_cov_4.665022, whole genome shotgun sequence.
ACCESSION   DAOTKS010000358 DAOTKS010000000
VERSION     DAOTKS010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58433_length_1676_cov_4.665022"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..>1676)
                     /gene="recJ"
                     /locus_tag="P5236_06475"
     CDS             complement(63..>1676)
                     /gene="recJ"
                     /locus_tag="P5236_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HRU64042.1"
                     /translation="INNFEEARKFFRPDLNDLHDPFLMADMDKAVDRLTKAMIKNEKI
                     LIYGDYDVDGTTSVAMVYKFIKQYHSRIGFYIPDRYTEGYGISYEGINYAARNHFSLV
                     IALDCGIKDMEKIAYAAKLNIDFIICDHHTTGETLPSSIAVLDPKRNDCLYPYKHLSG
                     CGVGFKLLQAFSFKNDIAFSDFIPLLEYVTLSIASDIVPITGENRILAYYGLKQINTS
                     PSIGLKEIINVCNLSDKEITISDIVFKIGPRINSSGRIKSASEVVELLISNDVNFSRK
                     KSEILNEYNNDRKNLDKNITDEAIQMIANDKEYYKKKSIVVYQPHWHKGVLGIVASRL
                     TDEYYKPAIVLGRSNGMVSGSGRSVQGFDLYKAIEYCSDLLENFGGHMYAAGLSLKEK
                     NIPLFAAKFEEYVSENILEEQTYPQMDIDCILQFKDITPKFFRILKQFAPFGPENMKP
                     VFVSKKVFDYGTSRLVGKEQEHLKMELIDSSSENVMNGIAFGMSEYNTHLKSLQPLNI
                     CYTLEENNFNGNTTIQLIIKDMKVENENF"
BASE COUNT          553 a          302 c          259 g          562 t
ORIGIN      
        1 tttcaaagag aaaagataag gatgacagtt cttcctcttg aaaaataata ttatttttca
       61 cttcaaaaat tttcgttttc gactttcatg tctttgatta tcagttgaat ggtagtattt
      121 ccattgaaat tattttcctc caaagtatag caaatattga gaggttgaag agattttaaa
      181 tgagtgttat attcactcat gccaaaagca attccattca tcacattttc ggagcttgaa
      241 tcaatcaatt ccatttttaa atgttcctgt tctttgccaa ccaaccgact tgttccataa
      301 tcaaaaactt tttttgaaac aaaaacaggt ttcatatttt cgggaccaaa aggtgcaaat
      361 tgttttaaga tacgaaaaaa tttgggtgta atgtctttaa attgaagaat acaatcaata
      421 tccatttgag gataggtttg ctcttcaaga atgttttctg aaacgtattc ctcaaatttt
      481 gcagcgaaca acggtatatt tttttctttt agcgataaac cagccgcata catgtgtccg
      541 ccaaaatttt caagtaaatc actacaatat tctatggctt tgtaaagatc aaaaccctgc
      601 acggaacgac ccgaaccaga aaccattcca tttgatcgtc ctaaaacaat agcaggttta
      661 taatattcat cagtgagccg tgaagctaca attcctaata ctcctttgtg ccaatgaggt
      721 tgataaacta caatagattt tttcttataa tattccttat cattggcaat catttgaata
      781 gcttcgtctg taatgttttt atccaaattt ttccgatcgt tattgtactc gttcaaaatt
      841 tcactttttt ttctggaaaa attcacatca ttggatataa gcaattctac aacttccgaa
      901 gcggatttta tccgtccgct ggaattgatg cgaggaccaa ttttaaaaac tatgtcacta
      961 atggtaattt ccttatctga cagattgcat acattgataa tttcttttaa accaatggaa
     1021 ggacttgtat taatctgttt cagaccgtaa taagctaaaa ttctgttttc accagtaata
     1081 ggaacgatat cagaagcaat gcttaaagta acatattcca acaaaggaat aaagtctgaa
     1141 aaagcaatat catttttaaa ggaaaaagct tgaagcaact taaaacctac accgcaccca
     1201 gaaagatgtt tatacggata aagacaatca ttacgtttgg gatctaacac tgctatggat
     1261 gagggaagtg tctcaccggt ggtgtgatga tcacaaataa taaaatctat attcaatttt
     1321 gctgcgtaag caattttttc catatccttg atgccacaat cgagagcaat caccaggctg
     1381 aaatgatttc tggctgcata attaattcct tcataagaaa ttccataacc ttctgtataa
     1441 cgatctggaa tataaaaacc tattctggaa tgatactgtt tgatgaattt ataaaccatt
     1501 gccacagagg tagtgccatc cacgtcgtaa tcaccataga ttaatatttt ttcattttta
     1561 atcatagctt tagtcaatct gtcgaccgct ttatccatat cagccatgag aaatggatcg
     1621 tgaagatcgt tcaggtcagg tctgaaaaac tttcgtgctt cttcaaaatt attaat
//
LOCUS       DAOTKS010000359         1674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_58543_length_1674_cov_5.100679, whole genome shotgun sequence.
ACCESSION   DAOTKS010000359 DAOTKS010000000
VERSION     DAOTKS010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58543_length_1674_cov_5.100679"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="P5236_06480"
     CDS             <1..890
                     /locus_tag="P5236_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007755552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HRU64043.1"
                     /translation="LQNDKKNFFSQNSVENDTENRHIKWYERGEHEHFDVCADDHCQR
                     YQGITRATSETVQLAIEVTRGEVLMFDHQICDTRFSKCCGGVTETFENCWAPVKHPYL
                     ESIVDNSLPPQGFDLDLAIESNAQKWIRECPPAFCNTQDKKVLSQVLNNYDQETIDFY
                     RWKVIYSQEEISEIIYRRSGFDFGKIIDLIPLKRGKSGRIVELKIVGSKQTMVVGKEL
                     EIRKWLSTSHLYSSAFVVDKENFRQQIPGRFILTGAGWGHGVGLCQIGAAMMSEKDYP
                     YDRILLHYFRETELRKIYE"
     gene            1325..>1674
                     /locus_tag="P5236_06485"
     CDS             1325..>1674
                     /locus_tag="P5236_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRU64044.1"
                     /translation="MSRIANNLTDLIGNTPLLRLSNYSKLKNLEAEIIVKIEAFNPCG
                     SVKDRVAKAMIEDAEKRGILKAGSEIIEPTSGNTGIGLAMVAAIKGYKLTLTMPETMS
                     IERRNLLKALGANIV"
BASE COUNT          589 a          242 c          321 g          522 t
ORIGIN      
        1 ctttgcaaaa tgataaaaag aatttttttt cacaaaattc tgtagaaaat gacacagaaa
       61 atcggcacat caaatggtac gaaagaggtg aacatgagca tttcgacgtt tgtgccgatg
      121 atcattgtca acgttatcaa ggaattacgc gagctacttc tgaaactgtt caacttgcaa
      181 ttgaagttac tcgcggcgaa gttttaatgt ttgaccatca aatttgtgat acacgatttt
      241 ctaaatgttg tggtggtgtt accgaaactt ttgaaaattg ctgggcgcct gtcaagcatc
      301 cttatttaga aagcattgta gataattctt tgccgccgca aggttttgat ctggatttag
      361 cgatcgaatc caatgcacaa aaatggattc gtgaatgtcc gccagctttt tgtaatacac
      421 aagacaaaaa agtcctttct caagttttga ataattacga tcaagaaaca atagattttt
      481 accgttggaa agtgatttac agtcaggaag agatttcaga aattatttat cgtcgttctg
      541 gatttgactt tggaaaaatt atagacttga tacctttgaa aagaggtaag tcaggcagaa
      601 ttgtggaatt aaaaattgtc ggtagtaaac aaactatggt ggtagggaaa gagttagaaa
      661 ttcgcaaatg gctgtcgaca agtcacttat acagttcagc ttttgtagtt gacaaggaaa
      721 attttcgtca acagattcct ggtcgtttta ttcttaccgg cgctggttgg ggacatggtg
      781 tgggactttg ccagattgga gctgccatga tgagcgaaaa agattatcct tacgaccgta
      841 ttttactgca ttattttcgt gaaacagagt tgaggaaaat ttatgaataa ctgtatcccg
      901 ctaaaacaaa gtagtttaaa aaaataaaat agttttttta agataactaa tagtcaagag
      961 tttaaagtcc tgagtagaga aatagaggat gtgcggttcg aaatatgagt tacaaaatct
     1021 ggtatatgaa aaatgaggat tttttgatcg gaaaaatttc gatttataac aaggagacaa
     1081 ttgaaacgaa acaaaaattt tcaaagtaat taaaatctaa aatccccaaa taagaatttt
     1141 tattcaacag cctattttca ataccttttt aaaagataat ttccaaaaat ttttcataat
     1201 ttttctttcg ttgataaaga tttaagataa ctttcgaatg attcaattca aataaatgtt
     1261 agttttacaa atagaacttc taactttgtg aattgataac tttttaatct tttaaaaaaa
     1321 taagatgtcc agaattgcaa ataacttaac agatttaatt gggaatactc ctttattgcg
     1381 gctttcaaat tacagtaaat taaaaaatct cgaagcagaa ataattgtta aaattgaagc
     1441 atttaatccc tgcggtagtg ttaaagatcg tgttgccaaa gcgatgattg aagatgctga
     1501 aaaacgagga attttaaaag ccggttcaga aattatcgaa ccaaccagcg gaaatactgg
     1561 tattggcttg gcgatggtag cagccataaa aggctataaa ttaaccctta ctatgcctga
     1621 aacgatgagc attgaacgaa gaaacctgtt aaaagctttg ggagccaata ttgt
//
LOCUS       DAOTKS010000360         1663 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_59109_length_1663_cov_3.718284, whole genome shotgun sequence.
ACCESSION   DAOTKS010000360 DAOTKS010000000
VERSION     DAOTKS010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59109_length_1663_cov_3.718284"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            643..1077
                     /gene="ruvX"
                     /locus_tag="P5236_06490"
     CDS             643..1077
                     /gene="ruvX"
                     /locus_tag="P5236_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRU64045.1"
                     /translation="MGRILALDYGRKRVGIAITDPLQISANALETVHVHEIIPFLEKF
                     LAKENVEKILIGYPLQMNGQESESMKYIKPFVASLQKKFPDIELIYVDERFTSTLAHK
                     AMLKGGLKKKDRQNKALVDKISATIILQSYLESIKNKNNISD"
     gene            1112..1663
                     /gene="def"
                     /locus_tag="P5236_06495"
     CDS             1112..1663
                     /gene="def"
                     /locus_tag="P5236_06495"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRU64046.1"
                     /translation="MILPIYVYGNPILRKVAKPISKDYPDLKNLVNNMYETMYHAEGV
                     GLAAPQIGLPIRLLVIDLAPLKKEDPELGNFKITMINPTILEQSSDLVSGEEGCLSIP
                     GIHENVLRPRKIKINYFDIDFNEYTEEFEDYKARVVQHEYDHLEGHLFTDKINPLRRQ
                     LLKSKLTGISKGKTPCNYQIKIV"
BASE COUNT          667 a          214 c          262 g          520 t
ORIGIN      
        1 attttttgtt ccaaatgtag ttaagaaaca aaaaaatact gtgtaataat tgactaatat
       61 ggtgtaatat ttatcattgg gaaattattt attattaatt gtacattaat atatcttaca
      121 tgcttttgta tcagcccttt aaatgatcga actggattat tattgaaaca ttaatttacc
      181 ttattaaaaa aggaatggag tagagaagaa aactcccaat tctgaaggaa gccgaaacat
      241 aaagcacgac agaaacttgt tgtaagcaca gtatctaacc acaacttatt atgattggaa
      301 aaggaaatat gaagaaggag aggtattggc acttatgcca gattattaaa tgtgaaaaga
      361 aagttactca aaaattgaaa aagaaaaacg aacgtttgaa gaaattgctt gtcaaaaaat
      421 atttagaaaa aaatgaaaac gaaaacctat aaaggtattg attttcaagt gattagctaa
      481 ataaaaactt gaaaaattta ttgattttca attatttaac taaaaatata acacactatt
      541 gaatatacaa aagtggattt agttgctagt tagtaaagaa attccaataa tatcagtgga
      601 aattgaaaat tatcacttaa ttttgtaaaa aattactcaa aaatgggaag aattttggca
      661 cttgattatg gaaggaaaag ggttggaata gcaataaccg atcctttaca aatttcggcc
      721 aacgctttgg aaacagtaca tgttcatgaa ataataccat ttttggaaaa atttctagca
      781 aaagaaaatg ttgaaaaaat cctgatcggt tatcctctcc aaatgaatgg gcaggaatca
      841 gaatcaatga aatacattaa gcctttcgtt gcctcccttc aaaaaaaatt tccagatatt
      901 gaacttattt atgtagacga acgttttact tcaactttag ctcacaaagc tatgctcaaa
      961 ggaggtttaa agaaaaaaga cagacagaat aaagctttag ttgataaaat atcagctacc
     1021 attattttgc aaagttattt agaaagtata aaaaataaaa ataacataag tgactaatta
     1081 ttaaaaaatt atattttatc gaattaaaac tatgatttta ccaatttatg tttatggaaa
     1141 tccaatttta cgaaaagttg ctaaacctat ttctaaagat tatcctgatt taaagaattt
     1201 agtgaataat atgtatgaaa caatgtatca tgcagaggga gttggactgg ctgctcccca
     1261 aattggtttg cctattcgcc tgttagtcat tgatctggca ccattaaaaa aagaagatcc
     1321 tgaattagga aattttaaaa ttacaatgat taatcccacc attttagaac aaagttctga
     1381 tttagtaagt ggtgaagaag gctgcctgag tattcctgga attcatgaaa atgttttaag
     1441 accgcgaaaa attaaaatta actattttga tattgatttt aatgaatata ctgaggaatt
     1501 tgaagattat aaagctcgcg tcgttcaaca cgagtatgat catctggaag gacatttatt
     1561 taccgataaa ataaatccat tgcgtcgtca acttttaaaa tcaaaattga caggcatttc
     1621 taaaggaaaa actccatgca attatcaaat aaaaattgtt tga
//
LOCUS       DAOTKS010000361         1650 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_59794_length_1650_cov_3.127273, whole genome shotgun sequence.
ACCESSION   DAOTKS010000361 DAOTKS010000000
VERSION     DAOTKS010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59794_length_1650_cov_3.127273"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..1082
                     /gene="cas1b"
                     /locus_tag="P5236_06500"
     CDS             66..1082
                     /gene="cas1b"
                     /locus_tag="P5236_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1b"
                     /protein_id="HRU64047.1"
                     /translation="MKKTYYLFNPGTLQRKDNTIKFTPIEEDENGIDHEGQPRYLPVE
                     DISEFYVFGALTANSALYNFLGQHDIAVHFFDYYENYTGSFMPRDSLLSGKMILAQTS
                     AYQNKKKRLELGRKFVEGAAFNMSKNLKYYSNRGKNLEENIEKIEQYVAMLPQTQAID
                     EIMGLEGNIRQTYYDGFDEIINDFSMGGRSKQPPKNEVNALISFGNMMCYSQCLRAIH
                     QTQLNPTISYLHTPGERRYSLSLDISEIFKPILVDRVIFKLLNKRELQEKHFESKLNK
                     CLLNPSGKKIFVQAFEERLNETIQHRSLKRKVSYRHLIKLECYKLSKHLLGMEEYKPF
                     KMYW"
     gene            1083..1346
                     /gene="cas2"
                     /locus_tag="P5236_06505"
     CDS             1083..1346
                     /gene="cas2"
                     /locus_tag="P5236_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HRU64048.1"
                     /translation="MYVILVYDVNEKRVAKMLKLCRRYLNWIQNSVFEGEISEVKLKE
                     LIIFAERIMKLEEDSLIIFSSRTEKFLDKQIIGKERSSTDIFL"
BASE COUNT          587 a          257 c          304 g          502 t
ORIGIN      
        1 gaaactatca aaaacactgt aaatcaccaa tcatccataa atgatcaaat catcacccat
       61 attcaatgaa aaaaacctat taccttttca atcctggcac cttgcagcga aaggacaata
      121 ccattaagtt tacaccgatt gaggaagatg aaaatggcat tgatcacgaa ggtcaaccac
      181 gttatttgcc ggtagaagat atttcagaat tttacgtgtt cggtgctttg actgccaata
      241 gtgctctgta taactttctg ggacaacatg atatagccgt acattttttt gattattatg
      301 aaaattatac gggctcattc atgccccgcg acagtttgtt gtctggaaaa atgattctgg
      361 cacaaacatc agcttatcaa aataagaaaa aacgattaga gctgggtcgt aaatttgtag
      421 aaggagccgc attcaacatg tcaaaaaatt tgaaatatta tagcaatcga ggaaaaaatc
      481 tggaagaaaa tattgaaaaa atagaacagt atgtggctat gttaccacaa actcaagcaa
      541 ttgacgaaat catgggactg gaaggcaata tccgacaaac ttattatgat ggttttgatg
      601 aaattatcaa cgatttcagc atgggaggaa gaagtaagca gccacctaaa aacgaagtga
      661 atgccttgat ttcgtttggc aatatgatgt gttattccca atgtttgcgt gctattcatc
      721 aaacacagtt gaatccaact atcagctatt tgcatactcc aggagaacgt cgttattcct
      781 tatcattgga tatttcggaa atttttaagc ccatactggt ggacagagta attttcaaat
      841 tgttgaacaa aagagagctt caggaaaaac attttgaaag taaactcaac aaatgtttgc
      901 ttaatccatc ggggaaaaag atttttgttc aagcattcga agaacggctc aacgaaacca
      961 ttcagcaccg atcgctcaaa cggaaagtga gttatcgaca tctcatcaag ttggagtgtt
     1021 ataaactaag caaacatttg ctggggatgg aagaatacaa accgtttaaa atgtattggt
     1081 aaatgtacgt aattttggta tatgacgtca atgaaaaacg agtagcaaaa atgctcaaac
     1141 tttgtcggag atatttgaac tggatacaaa attcggtttt tgaaggagaa atttcagaag
     1201 tcaaattgaa agaattaatt atatttgcag aaagaatcat gaaacttgaa gaagattcat
     1261 tgattatttt ttccagcagg acagaaaaat ttcttgacaa acaaatcatt ggaaaagaaa
     1321 gaagtagcac tgatattttt ttgtaatcaa ttgtcgaaag atgagttttt tctttttatg
     1381 ttgttgaatt cgaatatgat tttttttttg atctattgaa aatcaagata aaaacaagtt
     1441 gtcgaaaatc aaggattttt atatttttgc ctatcgacaa tttttacatt ccataaagga
     1501 tgctcattgt tttgctaaca atttgatttt tagataaaat ttttgataca gagcacggct
     1561 tttaatcgca ccagaaggaa ttgaaatttt tctgaatgct cgaaacggac tttgtattcg
     1621 ttcttttaat cgcaccagaa ggaattgaaa
//
LOCUS       DAOTKS010000362         1645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_60037_length_1645_cov_4.493711, whole genome shotgun sequence.
ACCESSION   DAOTKS010000362 DAOTKS010000000
VERSION     DAOTKS010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60037_length_1645_cov_4.493711"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="P5236_06510"
     CDS             complement(<1..253)
                     /locus_tag="P5236_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU64049.1"
                     /translation="MKQVKLILAGLLIGLISPILFLWLYLHFFFPLDLPFLEIIKKLS
                     PGILMGKLFLLSIVPDLFIGFIFYRLEHFKLASGVILGCI"
     gene            complement(258..1028)
                     /gene="surE"
                     /locus_tag="P5236_06515"
     CDS             complement(258..1028)
                     /gene="surE"
                     /locus_tag="P5236_06515"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRU64050.1"
                     /translation="MIMNKKLILITNDDGFDAKGIQVLTKLMTRFGDVVVVAPDVPRS
                     AQSNALTVTQPIRFRKIEENEQVIRYSCTGTPTDCVKIALNEILSQKPDLLVSGINHG
                     ANSAINVLYSGTIGAVLEGCVNRIPSIGFSLCSYQADPDFTNFEPYILQIVQTVLEKG
                     LPNDVCLNVNAPSGEIKGILITRQCKGFWTQEFAGRTDPAGRAYYWLTGYFENREPEA
                     EDTDEWALSHGYISVVPIKVDMTSYETIELLKQWNLEK"
     gene            complement(1207..>1645)
                     /locus_tag="P5236_06520"
     CDS             complement(1207..>1645)
                     /locus_tag="P5236_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HRU64051.1"
                     /translation="FLASWLLIILALIPTILYYFSVYYLAEPVGNVDVGSFWGSFIGL
                     LFLAGSYSAIGIFASSLSNHQMISFVVAVILCFFFFFGFELIASFFHSGEKIVFIENL
                     GIHAHYKSLSRGVIDSQDIIYFIVVIVVFLWAANLKIKREYHN"
BASE COUNT          613 a          313 c          209 g          510 t
ORIGIN      
        1 gtatgcaacc taaaataaca cctgatgcta acttaaaatg ttcaagccga taaaaaataa
       61 atccgataaa taaatctgga actatggaaa gcaaaaacaa tttccccatt aaaattccag
      121 gagataattt tttaatgatt tctaaaaaag gcaaatcaag tggaaaaaag aaatgcaaat
      181 acaaccataa aaaaagaatt ggagatatca agccaattaa aagtccagct aaaataagtt
      241 tcacttgctt catttaatta tttttctaaa ttccattgtt taagtaactc tatagtttcg
      301 taagacgtca tatcaacttt tataggaact acagaaatat agccatgact taaagcccat
      361 tcatcagtgt cttctgcttc tggttctcta ttctcaaaat atccagttaa ccaataatag
      421 gcacggcctg ctggatcggt tcttccggca aattcctgtg tccaaaatcc tttgcattga
      481 cgggttatca atatcccttt tatttcccct gatggtgcat tgacatttag acagacatca
      541 ttaggtaaac ctttttctaa aacagtttga actatttgaa gaatatatgg ttcaaaatta
      601 gtgaaatcag gatcagcttg ataactacac aaagaaaaac ctattgaagg aattctgttt
      661 acacaacctt ccagtacagc tcctatcgta ccagaataca aaacattaat ggctgaattt
      721 gctccatgat ttattcccga aactaacaaa tcaggttttt gactcaaaat ttcattcaac
      781 gcaattttta cacaatcagt gggcgttcct gtacaagaat aacgtatgac ttgctcgttt
      841 tcttctattt ttctaaatcg gataggctga gttacagtta aagcatttga ttgagcagaa
      901 cgaggcacat caggagcaac aaccactaca tccccaaagc gagtcattaa ttttgttagt
      961 acttggattc ctttcgcatc aaacccgtca tcattggtaa tcaaaatcaa ttttttattc
     1021 attatcatat ttccaaattt ataactttat cacttcaagc cttacttctt gtttttttct
     1081 ctttactctc aattatttac tcaacactcc cccctctcct atctcgtatt accttttcta
     1141 aaaaattatt actgccattt aaaatgatta ctcaaaacta aaaatctata aactgttaat
     1201 tattaactaa ttatgatatt ctcttttaat ttttagattg gcagcccata aaaacacaac
     1261 tattacaact ataaaataaa ttatatcttg tgaatctatg acgcctcgac ttaaagattt
     1321 ataatgagca tgaataccta aattttcgat aaaaactatc ttttctcctg aatgaaaaaa
     1381 agaagctatt aattcaaaac caaaaaagaa aaagaagcat aatatcactg ctactacaaa
     1441 agaaatcatt tgatgattag aaagagaaga agcaaatatt cctattgcag aataactacc
     1501 tgccaaaaat aacaatccaa taaatgagcc ccaaaaagaa ccaacatcta cattacccac
     1561 cggttctgct aaataatata cggaaaaata ataaaggatg gtgggtatta aagcaagaat
     1621 aattaaaagc cacgaagcta aaaat
//
LOCUS       DAOTKS010000363         1643 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_60117_length_1643_cov_4.554786, whole genome shotgun sequence.
ACCESSION   DAOTKS010000363 DAOTKS010000000
VERSION     DAOTKS010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60117_length_1643_cov_4.554786"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(420..>1643)
                     /locus_tag="P5236_06525"
     CDS             complement(420..>1643)
                     /locus_tag="P5236_06525"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HRU64052.1"
                     /translation="KNNPVIAQMEQQINGLKQTIISSIKNINNSLSISKNNLLKQESE
                     FISKIKNIPTQERQYLEIKRQQQIKEELYLFLLQKREENALNLASTIPSARIIDAAYT
                     GNMVAPRRLIIFLLAFILALALPVAIIYLKEILNNTITDRKEFQKLVQVPYLGSVGLT
                     KELDKIVVQEGKTTPIAEMFRLIRTNLQFMLPSDKKSPVILITSTISGEGKTFVAVNL
                     AMSLALMKKKVILLGLDIRKPMLDEYLQLPKNKGITIFLSDSNCQIKDIIHPSGIHPY
                     LDVIMAGPVPPNPAELLMNSRLDELIDELRIQYDYIILDTSPIGIVSDTFLLDRISDI
                     NICVARQNYSPREVCHLINTTYESKKLKNMAVILNGTDNISSYGYGLYKYHYGKKYKY
                     HEETKKTFISFLKKG"
BASE COUNT          536 a          243 c          241 g          623 t
ORIGIN      
        1 gttagaatta agttgaaaaa tctttatgta ggataactca tcattttggt tattggtaac
       61 tttgattcga gtgtattaat tcaatatact cactttctat tataaacaat actaccattg
      121 aagtgagaaa agttcaaaaa caggattctt cactgatttg tttagctttc tccttcctca
      181 aaatattttc aacaacctta tctattttta aaatataagt tattgatatt acatccgtta
      241 tattttttat agttttgtaa ttatttattc gcacagctat aaaaaaatgt tatttcccct
      301 atttaggaca ctactaaaat tttttaaatg aataaccccg aggcaagcct cgaggaattc
      361 ttttgattaa ataatttaga ctcatctacc aaatccatga aatcacttat tattattttt
      421 tatccttttt taagaaaaga aataaatgtt tttttggttt cttcgtgata tttatatttt
      481 tttccataat gatatttata caggccatat ccataacttg aaatattatc tgtaccattc
      541 aaaattacag ccatattttt taatttttta ctttcgtaag tggtattgat caaatggcat
      601 acttctcttg gagaataatt ttgacgagct acgcatatat ttatatcact gattctatct
      661 aatagaaatg tatcagatac gattccaata ggagaagtat ccagaataat ataatcatat
      721 tgtattcgta actcatcgat gagttcatct aatcttgaat tcataagtaa ttcagcagga
      781 ttaggtggaa cgggacctgc catgataacg tccagataag gatgaattcc cgaaggatga
      841 ataatatctt ttatttgaca atttgaatca gataaaaata tagtaatacc tttattttta
      901 ggcaattgca gatattcgtc cagcatcggc ttacgaatgt ctaagcctaa taagatcacc
      961 ttttttttca tcaaagcaag tgacatggct aaattgacag ctacaaaagt ttttccctcg
     1021 ccactgattg tagaagtaat aagaataacg ggtgattttt tatcagaggg taacataaat
     1081 tgtaaatttg tacgaataag gcggaacatt tctgcaatag gtgtagtttt tccctcttgt
     1141 actacaattt tgtctaattc ttttgttaag ccaacgcttc ccaaataagg aacttgtact
     1201 aatttctgga attcttttct atcagtgata gtattattaa gtatttcttt aagataaata
     1261 attgccacag gtaatgcaag agctaatatg aaagcaagaa gaaaaataat taatcttcga
     1321 ggagctacca tattcccagt ataagctgcg tcaataatcc gagcggaagg tatggtgctt
     1381 gcaagattca aagcattttc ttcgcgtttc tgtaataaaa ataaataaag ctcttcttta
     1441 atttgttgtt gacgttttat ttcaagatat tggcgttctt gcgtgggtat gtttttaatt
     1501 ttagaaataa attccgattc ctgctttaaa agattatttt tagaaatact taaactatta
     1561 ttaatatttt taatgctgct aataatggtt tgttttaacc cattaatttg ttgttccatt
     1621 tgagcaatta ctggattgtt ttt
//
LOCUS       DAOTKS010000364         1642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_60174_length_1642_cov_3.951481, whole genome shotgun sequence.
ACCESSION   DAOTKS010000364 DAOTKS010000000
VERSION     DAOTKS010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60174_length_1642_cov_3.951481"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..1495)
                     /locus_tag="P5236_06530"
     CDS             complement(152..1495)
                     /locus_tag="P5236_06530"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRU64053.1"
                     /translation="MDNIKLSIDNAFVTAEKIIEYQERVNECMVALHNHTGKGSDFLG
                     WLHLPSTIEDTLLKDIEDTAKNLREHCEIVVMIGIGGSYLGAKAVIDALSNSFEWLQP
                     KYKSPLIVFAGHNICEDYLYELQELLKNKKFGIICISKSGTTTEPALAFRLLKTQLEE
                     QQGKKEAQKLIIAITDKSKGALRKLAEQEGYKTFVIPDDVGGRFSVLTPVGLLPIAVA
                     GFNIRQLIAGAAQMEKICDIDVPFGENPATHYAVVRNELYKMGKKIEILVNYHPKLHY
                     ISEWWKQLYGESEGKENKGIFPAAVDFTTDLHSMGQWIQEGERIIFETVISIKEPNYL
                     KIFPYDKDNLDGLNFLAGKRIDEVNKMAELGTLIAHVDGDVPNIKIEIPKLNEYYLGE
                     LIYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEEQTRAIQARLKK"
BASE COUNT          507 a          310 c          234 g          591 t
ORIGIN      
        1 gtaatacctc gctgcttgcg gcgaggatag tcaattttaa ggaatttctt tattcccaat
       61 ttctcattaa atgagtagaa acatttcaca gaatgtctct actcgattca atcaaatcct
      121 cgtatctcgt atttcgcacc tttagaaatg cttatttttt taatcttgct tgaatagctc
      181 tggtttgttc ttcatagcca ggtttcccaa gtaaagcaaa catgtttttc ttgtatgctt
      241 ctacaccagg ctgatcgaaa ggattgacac ccaaaagata accactaatg ccacaagcct
      301 tttcaaagaa ataaatcaac tcacccaaat aatattcatt caatttcgga atctcaattt
      361 taatattggg aacgtctcca tccacatgag caatcaaagt gcctaattca gccattttgt
      421 ttacttcatc gattcttttg ccagcaagaa aattcaaacc atctaaatta tctttgtcat
      481 aaggaaaaat ttttagataa ttaggttcct taattgaaat tactgtttcg aagataattc
      541 gttctccttc ttgtatccac tgacccattg aatgtaaatc ggtagtaaaa tcaactgctg
      601 cagggaaaat acccttattt tcctttcctt cgctttcacc ataaagttgt ttccaccact
      661 cagaaatata atgtaatttc ggatgataat taaccagaat ttcaatcttt ttacccattt
      721 tataaagttc atttcttaca acggcatagt gagtagcagg attttctcca aaaggaacat
      781 ctatatcgca aattttctcc atttgagcag cccctgctat tagttgtcgt atattgaagc
      841 ccgcaacagc tattgggagt aaacctacag gagtaagaac tgaaaatcta cctccaacat
      901 catcaggaat gacaaatgtt ttataacctt cttgttctgc aagtttgcga agtgcacctt
      961 ttgatttgtc ggtaatagca ataattaatt tttgtgcttc tttttttcct tgctgttctt
     1021 ctaattgagt ttttaaaaga cgaaaagcta aggcaggttc agttgtagta cctgattttg
     1081 aaatacaaat aatgccaaat tttttattct tgagaagttc ctgaagttca taaagataat
     1141 cttcacaaat attgtgtcct gcaaaaacaa ttaatggtga tttatatttt ggttgcaacc
     1201 attcaaaact gtttgaaagt gcatcaataa cggcttttgc acctaaataa cttcctccaa
     1261 taccaatcat cactactatt tcgcaatgtt ctcttaaatt ttttgctgta tcttcaatgt
     1321 cttttaacaa tgtatcttca atagttgaag gtaaatgcaa ccaaccaaga aaatcgcttc
     1381 cttttcctgt gtggttatgc aaagctacca tacattcatt aacacgttcc tgatattcta
     1441 ttattttttc tgcagtgaca aatgcattgt caattgaaag tttgatatta tccattttct
     1501 attttatttt ttgaaaggta tgagtaatta aaaaaataat ctgaaaattt acctttgatt
     1561 ttttcaattt aattcgtcgg taagtttccg tatttcaagg ataggtgaac ttttattata
     1621 taaaatttgg taaaccgcat cc
//
LOCUS       DAOTKS010000365         1635 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_60531_length_1635_cov_5.784177, whole genome shotgun sequence.
ACCESSION   DAOTKS010000365 DAOTKS010000000
VERSION     DAOTKS010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60531_length_1635_cov_5.784177"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="P5236_06535"
     CDS             complement(<1..364)
                     /locus_tag="P5236_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HRU64054.1"
                     /translation="MVKPFVFVTNNPHKVEEVRAILSPSIKIVSLSDLGFFEEIPETA
                     DTLEGNALLKAKYIYEKYGLNCFADDTGLEIEALGGEPGVFSARYAGEGHNSYENRKK
                     VLNLMTHQTNRAACFRTVI"
     gene            536..>1635
                     /locus_tag="P5236_06540"
     CDS             536..>1635
                     /locus_tag="P5236_06540"
                     /inference="COORDINATES: protein motif:HMM:NF012435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HRU64055.1"
                     /translation="MTRKEKVFFDKLGIVTAATGSAVGLGNIWRFPYLLGQSGGGAFL
                     VVYLICVIFLGLPVMISEFSIGRMAQANAVTSFRSLAPNKKWWLIGVMGVLSAFFILG
                     FYMVVSGWTLEYIVQAITNSFANQNVADLSQAFTDFSTNTWRPLIWIVLFTAINCAIV
                     IAGVKKGIEKTTKFLMPLLFIIIITLGIRSVTLPRGINGLNFLFKFEFDKITSKVILS
                     AMGQAFFSLSLGMGCMITYGSYMNKSNNLSRTALEITLLDTLVAILASVAIFPAVFSF
                     GIDPTQGPQLVFITLPNVFPKMPGGYIWAILFFVLLALAALTSIISLLEVIVAYLNEE
                     FNMSRKTAAIGSSVLIFILAILSSFSMGIWKNV"
BASE COUNT          454 a          257 c          298 g          626 t
ORIGIN      
        1 caatcactgt tctaaaacag gcggctcgat tggtttgatg agtcattaag ttcaatacct
       61 tttttctgtt ttcataggaa ttatgtcctt caccagcata acgggcagaa aaaacaccgg
      121 gttcgcctcc aagagcttca atttccaaac ctgtgtcatc agcaaaacaa ttgagcccat
      181 atttttcgta tatgtatttt gctttgagta acgcatttcc ttctaaagtg tcagccgttt
      241 ctggaatttc ttcaaagaaa ccaagatcac tcaaacttac aattttgata gaaggcgata
      301 aaatggctct tacttcttcc actttatgtg gattattcgt tacaaaaaca aatggtttca
      361 ctattttctt tctttaacta ctttctgcaa agataataaa tttgtataat ttaaaatgtc
      421 agtattttta tattatattt tggatttccc ctttcttctt atactcaata aatttcatta
      481 tttttgtgac aattttttaa ggtaaccaaa ttaaagagta catttttcat tttttatgac
      541 aaggaaagaa aaagtctttt ttgataaatt aggaattgtt actgctgcta caggctctgc
      601 tgtaggatta ggaaatatct ggcgattccc ttatttgtta gggcaatcgg gaggtggtgc
      661 ttttttagtg gtttatttga tatgtgttat atttctcgga ttaccagtga tgatttcaga
      721 attttccatt ggcagaatgg ctcaagccaa tgcagttact tcttttcgta gtctggcacc
      781 caataaaaaa tggtggctta ttggtgtaat gggagtatta tcggcttttt ttatacttgg
      841 tttttatatg gtggtttctg gatggacact ggaatatata gttcaagcca ttaccaattc
      901 ttttgccaat caaaatgtag cagatttgag tcaagctttc actgatttta gcacgaatac
      961 gtggcgaccg ctgatttgga ttgtattatt tacagcgatt aattgtgcta ttgtgatcgc
     1021 aggagtaaaa aagggaattg aaaaaactac caagttctta atgcctcttt tgtttattat
     1081 tattatcact ttaggaatac gttctgttac gttgcccaga ggtataaatg gcttgaattt
     1141 tttgttcaaa tttgaatttg ataaaattac ttccaaagta attttaagtg caatgggaca
     1201 ggcttttttt tcattaagtt tgggaatggg ttgcatgatt acgtatggtt cttatatgaa
     1261 taaaagtaat aatttaagtc gaactgcact tgaaataacg ttactggaca ctttggttgc
     1321 tattctggct tccgttgcaa tatttcctgc tgttttttcc tttggcattg atcctacgca
     1381 aggaccacaa ttagtattta tcacacttcc taatgttttt ccaaaaatgc cgggcggata
     1441 tatttgggca attttatttt ttgttttact ggctttagct gctttgactt ccattatttc
     1501 gctattagaa gtaattgtgg cttatttgaa tgaagagttc aatatgagca ggaaaactgc
     1561 tgctattggc tcatccgtat tgatatttat attggctatt ctgtcttctt tttcaatggg
     1621 aatatggaaa aatgt
//
LOCUS       DAOTKS010000366         1634 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_60582_length_1634_cov_5.905636, whole genome shotgun sequence.
ACCESSION   DAOTKS010000366 DAOTKS010000000
VERSION     DAOTKS010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60582_length_1634_cov_5.905636"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="P5236_06545"
     CDS             complement(<1..224)
                     /locus_tag="P5236_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HRU64056.1"
                     /translation="MVSQKFRTRFHQPLKQWVKAGIWSLIYILFIAWVNNFWWLLLLP
                     FIFDIYITKFIPWTWWKKVKNKSLRSFLSWV"
     gene            complement(366..1082)
                     /gene="dapB"
                     /locus_tag="P5236_06550"
     CDS             complement(366..1082)
                     /gene="dapB"
                     /locus_tag="P5236_06550"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRU64057.1"
                     /translation="MKLALIGYGKMGKTIEKIARERGHKIVSIIDVNNLEDFDSENFR
                     QADVAIEFTNFKSALDNYKKAFEHNIPVVSGTTGINDELPLLKKEVEENGYTFLWSSN
                     FSLGVNILLAVNKYLSNIMNQFSEYDVDITEIHHTQKLDVPSGTAITLAEGIIEKLNR
                     KKKWVKDVQKNSDEIAIQSIRTDDVPGIHTVRYESQIDTIEITHNAKNRDGFAVGAVL
                     AAEFLAGKKGWFTMADFLKF"
     gene            complement(1183..>1634)
                     /locus_tag="P5236_06555"
     CDS             complement(1183..>1634)
                     /locus_tag="P5236_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRU64058.1"
                     /translation="KQKVEKSVHIKEAIVEKDPKEQNLRKALNLGHTFGHAFESFSLL
                     SNKPVLHGYAVMWGLLCELYLSVIRFNFPQEDLLKLKYLIKEYYGIFFIPCENYEKIY
                     ELMTHDKKNRSKEINFTLLSDIGQIQLNQTASKEEIFECLDFLSNGL"
BASE COUNT          576 a          297 c          200 g          561 t
ORIGIN      
        1 acccagctta aaaaagaacg tagactttta ttttttactt tcttccacca ggtccacgga
       61 ataaatttgg taatgtaaat gtcaaaaata aaaggtagca gtaacaacca ccagaaatta
      121 tttacccacg caataaacaa aatataaata agtgaccata taccagcttt tacccattgt
      181 ttaagaggct gatgaaaacg tgtcctaaat ttttgagaaa ccataaaaaa tttttattaa
      241 ttacaaatac tcctaattaa aactacttaa aaaaacagca ataaatcttt atgttaaaat
      301 atatttttca aaatgaataa ccctgaggca agcctcgaag aattcttttg attaaaaagg
      361 aaaaactaaa atttcagaaa atcagccata gtaaaccaac ctttctttcc tgccaaaaac
      421 tctgccgcca acactgctcc aacagcaaaa ccatcacgat ttttcgcatt gtgcgttatt
      481 tcaatggtgt caatttgcga ttcataacga acggtatgaa tgcctggcac atcatcagtc
      541 ctaatagatt gaatagctat ttcatcacta tttttctgaa catcttttac ccattttttc
      601 tttcggttca gtttttcaat aattccttca gccagagtaa tagctgtacc acttggtaca
      661 tctaattttt gggtgtgatg tatttcggta atgtcaacat catattcaga aaattgattc
      721 ataatgtttg acagatattt attgacggca agcaaaatgt ttactcctaa actgaaattg
      781 gaagaccaca aaaaagtata accattttcc tcaacttcct ttttcagcaa aggcaattca
      841 tcattgatgc cagttgtgcc tgaaactact ggaatattat gttcaaaagc tttcttataa
      901 ttatccaatg ctgatttaaa attagtaaat tcaatagcaa catcggcttg cctaaaattt
      961 tctgaatcga aatcttccag attgtttaca tcaataatgg aaacaatttt atgacctcgc
     1021 tctcgggcaa ttttttcaat ggttttcccc attttgccat atccaatcaa tgctaacttc
     1081 atattaaaaa ttgatatttt tacattttat attaaaagta aatcattaat tttcaaattt
     1141 ttagtgttag ttagtattta catagtccta gtgtacaggt cttcataaac cgttacttaa
     1201 aaaatctaaa cactcaaaaa tttcttcttt actggcagtt tgattaagtt gtatttgacc
     1261 aatatctgac agtaaagtaa aattaatttc tttcgatcga ttttttttat cgtgcgtcat
     1321 taattcataa atcttttcat aattttcgca aggaatgaaa aaaataccat aatattcttt
     1381 tatcaaatat tttaatttca acaaatcttc ttgaggaaaa ttaaatctga taacagaaag
     1441 atataattca cacaacaatc cccacattac agcataacca tgcaaaacag gtttattaga
     1501 aagaagagaa aaactttcga aagcatgacc aaaagtgtga cccaaattca aagcttttct
     1561 caaattctgt tccttcggat ctttttctac aatagcttct ttaatatgaa cacttttttc
     1621 tactttctgc ttta
//
LOCUS       DAOTKS010000367         1630 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_60819_length_1630_cov_4.271746, whole genome shotgun sequence.
ACCESSION   DAOTKS010000367 DAOTKS010000000
VERSION     DAOTKS010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60819_length_1630_cov_4.271746"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="P5236_06560"
     CDS             <1..512
                     /locus_tag="P5236_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRU64059.1"
                     /translation="YANQNAYDCVQIHGGSGFMKDYPCERLYRDARILTIYEGTSQLQ
                     VVAAIRHVTTGTYLNQIRFYEQQPINPEFHSFRKRLITMTNLLENSINRVTETKNQEY
                     IDFHARRLVEMAGHIIMGYLLIIDASRNDMFRKSAEVYINYAEVETRKHATFIKTFDM
                     ENIDKYKVI"
     gene            complement(654..1388)
                     /locus_tag="P5236_06565"
     CDS             complement(654..1388)
                     /locus_tag="P5236_06565"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HRU64060.1"
                     /translation="MIFPLLLTKGYSQNDIKIIYNLKLGLINGGTATMIKKDTLYNNK
                     KCIYYLLEGNTDGLTDKIFSVHEKYESIVDASTHLPYVALRKAKERSYQYYNKVYFYR
                     ENDSIYSERTGGCKVPKDLTDILSVFFYFVNTYDMDKMEEGNAITIPTINGHKIDDLK
                     ITFVKNQVLKNELGTEICYLLTPKVKKGKVLKSSDGLKFYLSQKEKIPIQIEIETKVG
                     TLKAVPIQMYCGQKPLKLYCSKNCGN"
BASE COUNT          559 a          237 c          236 g          598 t
ORIGIN      
        1 aatatgccaa tcagaatgct tacgattgtg tacaaataca tggtggttct ggatttatga
       61 aagattatcc ttgcgagcga ctatatcgtg atgcacgtat tcttactatt tatgaaggaa
      121 cttctcagct tcaagttgtt gctgctattc gtcatgtgac aacaggtacc tatttaaatc
      181 aaattcgttt ttacgagcaa caacctatta atccagaatt tcattcgttt cgtaaaagat
      241 tgataacaat gacgaatttg ttggaaaatt cgatcaacag ggtaactgaa acaaagaatc
      301 aagagtatat tgattttcat gctcgccgat tggtcgaaat ggcaggtcac attatcatgg
      361 ggtatttatt gattattgat gcttcaagaa atgatatgtt tcgtaaatcg gctgaagttt
      421 atattaatta tgctgaagtg gaaactcgca aacatgcaac ctttatcaaa actttcgata
      481 tggaaaatat tgataaatat aaggtaattt aaattttttg tgagagggta tttcagaatt
      541 ttaaaaaagt ttttttaatt tatctctaat tacaaagaaa gcattacaag tgaaccactt
      601 agaatgcttt ctttggtata aggggaaaga tattttttag taaagatcaa attttagttc
      661 ccacaatttt tagaacaata caatttcaaa ggcttttgcc cgcaatacat ctggatggga
      721 acagctttta aagtacctac tttggtttca atttcaattt gaataggtat tttttctttt
      781 tgagaaaggt aaaacttcaa accatcagaa ctttttaaca ctttcccttt tttcacttta
      841 ggagttaata aataacaaat ttctgtacca agctcatttt ttaaaacttg atttttgaca
      901 aaggtgattt ttaaatcatc aattttatga ccgttaatag ttggaatggt aatggcattt
      961 ccttcttcca ttttatccat atcataagtg ttaacaaagt aaaagaaaac ggacaaaata
     1021 tccgtcaaat cttttgggac tttacagcct ccagtacgtt cactataaat agaatcattt
     1081 tcacgataaa aatatacttt attgtaatat tggtaagaac gttccttcgc tttacgaaga
     1141 gctacataag gcaaatgcgt agaagcatca acaatactct catatttttc atgtacggaa
     1201 aatattttat cagttaatcc atctgtattt ccttcaagta gataataaat acatttttta
     1261 ttattataga gagtatcttt ctttatcata gtagcggtgc cgccatttat caaccccaat
     1321 ttcaaattat aaataatttt aatgtcgttt tgcgaataac cttttgttaa taaaagaggg
     1381 aaaatcatta caattaaaaa tttttttcta aagaagtaga aatttgaata tttcataatt
     1441 attttatctt aaatttacaa aaatttttta ttaggctctt tcttatttaa ttaattcctt
     1501 catttacata atacaacatt attaacaaat atcataccta aaaagtcttt aaaaaaattc
     1561 atttttttga tagctagcta agttcaacat agaagtgcga atttagcaga gatgttttgc
     1621 atgatttttt
//
LOCUS       DAOTKS010000368         1626 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_61050_length_1626_cov_3.154679, whole genome shotgun sequence.
ACCESSION   DAOTKS010000368 DAOTKS010000000
VERSION     DAOTKS010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61050_length_1626_cov_3.154679"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..1372
                     /gene="xseA"
                     /locus_tag="P5236_06570"
     CDS             143..1372
                     /gene="xseA"
                     /locus_tag="P5236_06570"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRU64061.1"
                     /translation="MNHITLVELTEQIQETININFDQPVWVEAEISELRENPSGHCYL
                     DLIEKEKNSDVLLARIKANCWSPVYRILKPYFEDTTGQSLRSGLKILISVTVDYHPLY
                     GISLTIHDIEPSFTIGELALRQMEIIRQLEEEGIADMNKQLPFPLLPQRLAVISSPTA
                     AGYGDFCDQLKNNPYSFVFYTKLFPAIMQGEQAANSIISALEKIYQYVDLFDLVVIIR
                     GGGATADLSCFDSYELALHCAQFPLPILTGIGHQRDISILDMMAHTSLKTPTAVADFL
                     ISNLQEAENQLKNLISNISFFAKQKVEMEKNTLNITRFQLKQTLKTWLIQKNHELEKE
                     KNRLKNAIHFALAKEQNKLGMYEKIIQTHSPAFLLKYGYTITTLKGQKITSVEQVKKG
                     DCIQTYVIDGNFESEVL"
     gene            1410..>1626
                     /locus_tag="P5236_06575"
     CDS             1410..>1626
                     /locus_tag="P5236_06575"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: protein motif:HMM:NF014233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HRU64062.1"
                     /translation="MTKISLENMEFHAFHGCLEHEQKFGNFFFVSVTMEVDTLLADES
                     DQLVDTIDYQQVYNVVKEEMGKPSKLLE"
BASE COUNT          563 a          241 c          281 g          541 t
ORIGIN      
        1 aagctatctc ttgggaaaca atattcgatt gtacaatggc tgatattaat atttttttca
       61 attgaaatta aaatatttgc ataataacaa tttggtattg cctatcaaca tctttataaa
      121 actttacatt tagttaaaaa caatgaatca catcactctt gttgaattaa ccgagcaaat
      181 tcaggaaacg ataaatataa atttcgacca acctgtttgg gttgaagcag aaataagcga
      241 actgcgagaa aacccaagtg gacattgtta tttagatttg attgaaaaag aaaaaaattc
      301 tgatgttctt ttagcgagga taaaagctaa ctgctggtca cctgtttatc ggattttaaa
      361 accttatttt gaagatacaa cagggcaatc acttcgttcg ggactaaaaa ttttaatctc
      421 agtaactgtt gattatcatc cactttatgg tatcagttta actatccatg atattgagcc
      481 ttcgtttact ataggcgaat tggcattgag gcaaatggag attatccgtc aattagaaga
      541 agaaggaata gctgatatga ataaacagtt accgtttcca ctgctcccgc aacgtttggc
      601 agtgatttct tcgcccactg ctgctggtta tggtgatttt tgtgaccaat taaagaataa
      661 tccttattca ttcgtttttt atactaaatt atttcctgct attatgcaag gtgaacaagc
      721 tgctaattcc attatttcgg ctctggaaaa aatttatcaa tatgttgatt tgtttgattt
      781 ggtagtgatt atcagagggg gcggtgcaac ggctgattta agttgctttg attcttatga
      841 gcttgctctt cattgtgctc aatttccttt gcctatttta acagggattg gtcatcaacg
      901 cgatatttca attttagaca tgatggctca tactagttta aagacaccaa ctgctgtagc
      961 agatttctta atttcaaatt tacaggaagc tgaaaatcag ttaaaaaatt taatttcaaa
     1021 tatttcattc tttgcgaagc agaaagttga aatggaaaaa aatactttaa atattacccg
     1081 ttttcagttg aagcaaactt taaaaacttg gttgatacaa aaaaatcatg agctcgaaaa
     1141 agaaaaaaat cgcttaaaaa atgcaattca ttttgctctg gctaaagaac aaaataaatt
     1201 gggaatgtat gaaaaaatta ttcaaaccca ttcacctgct tttttgctga aatatggtta
     1261 taccattact actttgaagg gacagaaaat aacttcagta gaacaggtaa aaaagggaga
     1321 ttgtattcaa acttatgtaa tagacgggaa ctttgaaagt gaagtacttt aagcttattt
     1381 aaaaatataa atttttgaaa caaattatta tgactaaaat atccctcgaa aatatggaat
     1441 ttcatgcttt tcacggttgt ttggagcatg aacaaaaatt tggcaatttt tttttcgtaa
     1501 gtgtaactat ggaagtagat actttattag ccgacgaatc agatcaattg gtagatacga
     1561 ttgattatca acaggtgtat aacgttgtaa aagaagaaat gggaaagcct tccaaacttt
     1621 tagaac
//
LOCUS       DAOTKS010000369         1620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_61342_length_1620_cov_4.109265, whole genome shotgun sequence.
ACCESSION   DAOTKS010000369 DAOTKS010000000
VERSION     DAOTKS010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61342_length_1620_cov_4.109265"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="P5236_06580"
     CDS             <1..558
                     /locus_tag="P5236_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HRU64063.1"
                     /translation="REVSNPSELLLSQNHEGLSGIAIASAMEGIRPFMIETQALVSSA
                     AYGMPQRSCTGFDIRRLNMLLAVLEKRVGFKLSQKDVFLNIAGGLKVNDPAIDLAIIS
                     AILSSNIDVAIERHVCMAGEVGLSGEIRPINRIQQRIHEAEKLGFTKMIVPYSNLKKN
                     EIEKIKIEIIQVKKVEEAFKVLFKK"
     gene            complement(674..749)
                     /locus_tag="P5236_06585"
     tRNA            complement(674..749)
                     /locus_tag="P5236_06585"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(712..714),aa:His,seq:gtg)
     gene            856..1536
                     /locus_tag="P5236_06590"
     CDS             856..1536
                     /locus_tag="P5236_06590"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HRU64064.1"
                     /translation="MGIAGNQIEQTVEEILQKIKKSMNGVIASQMNKNGIIYQKNYGV
                     TIPRLKEIAAQYESQHELAQKLWEFPIRETKILATLLEPAEKFSYSMAQQWMEECTQI
                     ELIEQTCMNLFSRLSYASQLCCEWISSENDWKKIAGFILSSRISNTLTSEEKNFITEK
                     AFENSTTENYHLYKAISICLSRFCHTSKETTEEILQQIESFFDPLSTSQHYIKDSVEQ
                     ELNFLELL"
BASE COUNT          587 a          261 c          278 g          494 t
ORIGIN      
        1 cgcgaagtta gcaatccttc tgaattatta ctttcgcaaa accacgaagg actaagtggt
       61 atcgctattg catcagcaat ggaaggaatc cgtcctttca tgattgaaac acaggcatta
      121 gtaagttctg ctgcttatgg tatgcctcaa cgttcctgta caggttttga catacgtcgg
      181 ttaaatatgc tgctggcagt gctggaaaaa cgggtaggtt ttaaattgtc acaaaaagat
      241 gtttttctga atattgcggg tggtttaaaa gttaatgatc ctgcaattga tttagctata
      301 atttcagcta ttctttcttc caatattgat gtggcaattg aaagacatgt ttgtatggct
      361 ggcgaagtgg ggctttcagg cgaaatccgt ccaataaata gaattcagca acgcatccat
      421 gaagcagaaa aattaggatt taccaaaatg attgttcctt attctaactt gaaaaaaaac
      481 gaaatagaaa aaataaaaat agaaattatt caggtaaaaa aagtagaaga agctttcaaa
      541 gtgttgttta aaaagtaaat ttcttctccg tagacacaaa ataacaatca gaactttttg
      601 atttttatgg taagtgtttt aggtgccact tcatatttac aaaaacaaaa aagcagcttt
      661 tcgctgcctc ttcgggtgga ggacgggact tgaacccgcg acattcggaa ccacaatccg
      721 acgctctaac caactgagct acaaccacca tttttggtag tgcaaagata atatagtatt
      781 tgcttttatg caactatttt taaaaggaaa aactttttgc gattatttct tttatcacta
      841 aaatgtaaaa aaataatggg aatcgctgga aatcaaattg aacaaacggt tgaggaaatc
      901 ttacaaaaaa taaaaaaatc aatgaatggt gttatcgctt ctcaaatgaa taaaaatggc
      961 attatttacc aaaaaaatta tggtgttacc attcctcgac tgaaagaaat tgctgcacag
     1021 tatgaatcgc aacacgaatt agctcaaaaa ctttgggaat ttcctatccg cgaaacaaag
     1081 attttagcaa ctttattgga acctgcagaa aaattcagtt attcgatggc acaacaatgg
     1141 atggaagaat gtactcaaat agaattaatt gaacaaacct gtatgaattt attttcgcgt
     1201 ttatcgtatg cttcccaact ttgttgcgaa tggatctcgt ccgaaaatga ttggaagaaa
     1261 atagcaggtt ttatactttc ttcaaggata agcaatacgc taaccagtga agaaaaaaat
     1321 ttcattactg agaaagcttt tgaaaattca accacagaga attatcattt atacaaagcc
     1381 attagcattt gtttaagtag attttgtcat acaagtaaag aaacaactga agaaatttta
     1441 caacaaattg aatcattttt cgatccgctt tctactagtc aacattacat aaaagactca
     1501 gttgagcaag aattaaattt tctggaacta ttataaaaaa cacagaaaaa aatactacct
     1561 ttcctctata aatcataggt taattttttg aataagataa tcgtattatt ttgataatca
//
LOCUS       DAOTKS010000370         1618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_61469_length_1618_cov_3.682022, whole genome shotgun sequence.
ACCESSION   DAOTKS010000370 DAOTKS010000000
VERSION     DAOTKS010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61469_length_1618_cov_3.682022"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..606)
                     /gene="hisIE"
                     /locus_tag="P5236_06595"
     CDS             complement(13..606)
                     /gene="hisIE"
                     /locus_tag="P5236_06595"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HRU64065.1"
                     /translation="MEIDFKKMNGLVPAIIQDECTSKVLMLGYMNEEALAKTLETGKV
                     TFFSRSKNRLWTKGEESGNFLNLVSISPDCDNDTLLIKVHPEGPVCHTGKDTCWGESN
                     EGDFAFLKYLQEFIQKRFKEMPEGSYTTSLFKEGINRIAQKVGEETIETIIEAINSNK
                     EGFIYEASDLIYHLIVLLTAQGLTLDDIGKELKRRHK"
     gene            complement(969..>1618)
                     /locus_tag="P5236_06600"
     CDS             complement(969..>1618)
                     /locus_tag="P5236_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HRU64066.1"
                     /translation="RVISEEERNLEFLKKIIKQIYAAFLRTEYLVWESFPQIEPYLPQ
                     EIVFIHAEELRQLYPHLSPKEREIEFTKIHKAVFLIGIGAPLGDGKKHDGRAPDYDDW
                     STIAENGLPGLNGDILFWNPVLETAFEISSMGIRVDKTALLRQLEMEGAQDRLQMYFH
                     KCLLDGLLPLSIGGGIGQSRLCMFFLRKAHIGEIQSSIWPKEMQEKCAKMNIILM"
BASE COUNT          520 a          298 c          219 g          581 t
ORIGIN      
        1 gttatcaact tcctatttat gccttcgttt taattctttt ccaatatcat caagagttaa
       61 tccttgtgca gtcaacaaaa caatcaagtg ataaattaag tcggaagctt cgtaaataaa
      121 accttcttta ttactattta ttgcttctat tatggtttct attgtttctt cacctacttt
      181 ttgagctatg cgatttattc cttctttgaa caaagaagta gtataggaac cctcgggcat
      241 ctctttaaaa cgtttttgaa tgaattcttg caaatatttt aaaaaagcaa aatcaccttc
      301 attcgattct ccccagcaag tatctttgcc cgtatgacaa acaggacctt caggatgaac
      361 ttttatcaat aatgtatcat tatcacaatc gggactaata gaaaccaggt tcaaaaaatt
      421 gccactttct tctccttttg tccagagacg atttttgctt ctactgaaaa aagtaacttt
      481 ccctgtttca agagttttag ccagtgcctc ttcattcata taacccaaca tcaatacttt
      541 tgaagtgcat tcatcctgaa tgatggctgg tactaaacca ttcatttttt taaagtcaat
      601 ttccatttca tttgattttg ttaaaacgat aagcgaaagt acgaaaaaat agcttataat
      661 tacttgcaaa aggataaaaa gtttcaagaa tatcttttaa tattctgatt ttgttagggt
      721 tactaaatta ttttttgaat tcaattttag tcaaataaga aatttcttat tggttttcat
      781 ggttttcaaa tcttttaaaa acaaatttta ctttacctta tcttttttct tttgcctaaa
      841 caagcaaact actttttaga aaataatttg atagggttga ttcctttttt gctttaaatt
      901 ttcataaaaa atccccaaaa agttgtttct tctggggata atacaaatta aatatgcaaa
      961 agaaaaaatt acatgagtat gatattcatt ttagcacatt tttcctgcat ttccttcggc
     1021 catatacttg attgaatttc tccaatatga gcttttcgca gaaaaaacat acataaacgt
     1081 gattgtccta ttccaccacc tatcgaaaga ggtaacaaac catccagcaa acatttgtga
     1141 aaatacattt gtaaccgatc ttgtgcccct tccatctcta actgccgtaa aagagctgtc
     1201 ttgtcaacac ggatgcccat agaagaaatt tcaaaagcag tttctaaaac aggattccaa
     1261 aaaagaatat cgccatttaa acctggcaaa ccattttctg ctatagtgga ccaatcatca
     1321 taatctggtg cacgcccatc atgttttttg ccatcaccca gaggtgcacc tattccaatc
     1381 agaaagactg ctttgtgaat tttagtaaat tctatttctc tttcttttgg tgaaagatga
     1441 ggataaagtt gacgaagttc ttcggcatga ataaatacaa tttcttgagg caaataaggt
     1501 tcaatctgtg ggaagctttc ccaaactaaa tattcagtcc ttaaaaaagc agcataaatt
     1561 tgttttataa ttttttttaa aaattcaaga tttcgttctt cttcgctgat tactcttt
//
LOCUS       DAOTKS010000371         1615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_61646_length_1615_cov_2.846154, whole genome shotgun sequence.
ACCESSION   DAOTKS010000371 DAOTKS010000000
VERSION     DAOTKS010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61646_length_1615_cov_2.846154"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..1194
                     /locus_tag="P5236_06605"
     CDS             67..1194
                     /locus_tag="P5236_06605"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRU64067.1"
                     /translation="MKHYKFLLFAFVLLLALISYVSCRGWQVLTLIHSTVGRKSYLIV
                     CILLTICLFFGMFFEDKLSISLARPLLFLGYSFLLLVTYLFFSFLLVDIFRGLNHFLH
                     FISDQGMIALRFWTMMASLVLIMVLMIAGNIKFNHPALVNLYLKSNKSVQNKEIKIVA
                     VSDLHLGTCIDKKQLQKYITLINAQHPDLVLMVGDICDRSLPAMVHQNLKEDLQKIQA
                     PLGVYAVDGNHEYYSFHLAEVNEYYKNAGVNVLRDSTVEIQHQFYLVGRDDYTNRQRK
                     KLSELMDSFDPSKPVIVVDHQPMNLEESQEENIDLHLSGHTHNGQVFPGNLLAKRFFE
                     VSYGYKKKRNTHIYVSSGLGLWGPSYRIGTQSELVAIHFQY"
     gene            complement(1324..>1615)
                     /locus_tag="P5236_06610"
     CDS             complement(1324..>1615)
                     /locus_tag="P5236_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU64068.1"
                     /translation="MSAEERANYWAKELNLTDEEKAKVTELFKKEDENRAKYREEMQR
                     NREEMRAQMQKDRKANDAELEKIIGKEKFQKYDSIRSEQMKNRKRTPTTKGK"
BASE COUNT          480 a          261 c          276 g          598 t
ORIGIN      
        1 aaacttttga gctggaatta tttgaataag gaatcaatct taaccagata aaaaaattat
       61 tatgaaatga aacattataa attccttcta tttgcctttg ttctgcttct tgctttgatt
      121 tcgtatgtga gttgtcgtgg ctggcaggtt ttaacattga ttcattcaac tgtggggcga
      181 aaaagctatt taattgtctg tatccttttg actatctgcc tattttttgg gatgtttttt
      241 gaggacaagt tgtctatttc attagccaga cctctccttt ttttgggtta ttctttttta
      301 ctattggtaa cttatttgtt cttctcattt ttgttagttg acatttttcg cggactgaat
      361 cattttctgc attttatttc agatcaagga atgatagctt tgcgattttg gacaatgatg
      421 gcaagtttgg ttttgattat ggtattaatg atagctggaa atattaaatt caatcatccc
      481 gctttagtaa atttatattt aaagtcaaat aagtctgttc aaaataagga aataaaaatt
      541 gttgcagtaa gtgatcttca tttaggaact tgcattgata aaaagcaatt acaaaaatat
      601 ataacgctta tcaatgcaca acatcccgat ctggtcctga tggtaggtga tatttgtgat
      661 cgttctctgc cagcaatggt tcatcaaaat ttaaaagaag acttacaaaa aattcaagct
      721 ccattgggtg tttatgcagt ggatggaaat catgaatatt attctttcca tttagcagaa
      781 gtaaatgaat attataaaaa tgcaggagtg aatgttttac gtgatagtac cgtagaaatt
      841 cagcaccagt tttatctggt tgggagagat gattacacca atcgacaacg aaagaaactt
      901 tctgagttga tggattcttt tgacccttcg aaaccagtca tagtagttga tcatcagccg
      961 atgaacctgg aagaatcaca agaggaaaat attgaccttc atttatcagg acatacccat
     1021 aatggtcagg tttttccggg caatttgctg gcaaagcgat ttttcgaagt gagttatggt
     1081 tataagaaaa aaaggaatac acatatttat gtaagttcgg gactggggtt atggggacct
     1141 tcttatcgaa ttggtactca atcagaactg gtagcaattc attttcaata ctaaagaaaa
     1201 aataaaaaat tcatctgtaa ttttaaaaat aaaaactccc tttgaaagag tatattcaaa
     1261 aggagtttta ccttaaaaat acctaattgt acaatttttt aaaacctcaa ttttcacgat
     1321 aaattatttt ccttttgtgg taggcgttct tttcctgttt ttcatttgtt cagaacgaat
     1381 agaatcatac ttctgaaatt tttcttttcc aatgattttt tccagttcgg catcattggc
     1441 tttacgatct ttttgcattt gagctcgcat ttcttcacga tttctctgca tctcttcgcg
     1501 atacttcgct ctgttttcat cttccttctt gaaaagctcc gtaactttag ctttttcttc
     1561 gtcagttaaa tttaactcct ttgcccaata attagctcgt tcctcagcac tcatt
//
LOCUS       DAOTKS010000372         1595 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_62711_length_1595_cov_4.641558, whole genome shotgun sequence.
ACCESSION   DAOTKS010000372 DAOTKS010000000
VERSION     DAOTKS010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62711_length_1595_cov_4.641558"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /locus_tag="P5236_06615"
     CDS             <1..1192
                     /locus_tag="P5236_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRU64069.1"
                     /translation="SISTFIIEKSYETPKGKYTSINGNKAVAWGLIAAAEKAGKQLFL
                     GSYPITPASDIMHELALRKDIGVKMVQAEDEIGGITMAIGASFAGCLAATSTSGPGMA
                     LKSEGMGLAVMAELPLVIIDVMRSGPSTGMPTKTEQSDLFQALYGRSGESPLVVIAAS
                     TPDNCFNYAYIASKIALEYMTPVILLTDGYLGNGSSLWKLSNLAELPEITPHYVKSEM
                     EGCKVTIRDEKTKIPYRSIPGMKGFCHCNGGLEKNYTTGNLSTDPINHEKMVLARQEK
                     INNVANIIPDIQIAGNKDADLLVVGWGSTYGHLISATNLLNNEGYKVALAHFNYLFPL
                     PKNTEEVIKRYKKVVVCELNTGQFAAYLSSKISQVNFFKYNRIQGQPFTVNELVAYFK
                     QLLS"
     gene            1222..>1595
                     /locus_tag="P5236_06620"
     CDS             1222..>1595
                     /locus_tag="P5236_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRU64070.1"
                     /translation="METHNIQKYTVADFKSDQTPRWCPGCGDYGIYNSLLKTMVESGI
                     PPYETVIISGIGCSSRLPYYTKTYGFHTIHGRGAAIATGVKVANPQLTVWQITGDGDC
                     LAIGGNHFIHSIRRNVDINVLI"
BASE COUNT          541 a          254 c          311 g          489 t
ORIGIN      
        1 atccatttca acttttatta ttgaaaaatc atacgaaaca ccaaaaggaa aatataccag
       61 tattaatgga aacaaagctg tggcgtgggg attaattgct gctgctgaaa aagcaggtaa
      121 acagttgttt ttaggaagtt accctattac tcctgcttca gatatcatgc acgaattagc
      181 cctgagaaaa gatattggag taaagatggt tcaggcagaa gatgaaattg gaggtattac
      241 tatggctatt ggtgcctctt tcgcaggttg tctagcagca acaagcactt ccggaccagg
      301 aatggcttta aaatcagaag gaatgggatt ggccgtaatg gcagaattgc cccttgtgat
      361 tattgatgtt atgcgaagcg gaccttctac aggaatgcct accaaaacag aacaaagcga
      421 tctttttcaa gctctttatg gaagaagtgg cgaaagtcct ttggtcgtaa ttgcagcaag
      481 tacacccgat aattgtttca attatgctta tattgcaagc aaaattgcat tggaatatat
      541 gactcctgta attctactga ctgatgggta tttggggaac ggctcctctt tgtggaaatt
      601 atccaacctt gcagaattac cagaaataac tcctcattat gtaaaatcag aaatggaagg
      661 atgtaaggta actatacgtg atgaaaaaac aaaaattcca tatcggagta ttcctggaat
      721 gaaaggattt tgccattgta acggtggatt agaaaaaaat tacactactg gaaatttatc
      781 taccgatcct attaatcatg aaaaaatggt attagccaga caagaaaaaa taaataatgt
      841 ggcaaatata attcctgata ttcaaattgc aggaaacaaa gatgctgatt tattggtcgt
      901 tggttgggga agcacttatg gacatttaat cagtgctact aatttattaa ataatgaagg
      961 ttataaagta gcattggcac attttaacta tttgtttccc ttaccaaaaa atacggaaga
     1021 agttatcaaa cgctataaaa aagtagtcgt ttgcgaatta aatacgggac aatttgcggc
     1081 ttatttatct tcaaaaatta gtcaagttaa ctttttcaaa tacaatagaa ttcaaggaca
     1141 accttttact gtaaatgaat tggttgcata tttcaagcaa ttactttctt aattttttca
     1201 aaaaaataga aacttttgac tatggaaaca cataatattc aaaaatatac tgttgcagat
     1261 ttcaaaagtg atcaaacacc tcgttggtgc cctggttgcg gtgattatgg tatttacaat
     1321 tccttattaa aaacgatggt tgagtcagga atccctcctt atgaaactgt aattatttct
     1381 ggaattggct gctcttcacg tttgccctat tacacaaaaa cttatggatt tcataccatt
     1441 catggaagag gggctgctat agcgacaggt gttaaagttg ctaatcctca acttactgtt
     1501 tggcaaatta caggcgatgg ggattgtctt gcaataggtg gaaaccattt tattcatagt
     1561 ataaggagaa atgtagatat caacgtactt atttt
//
LOCUS       DAOTKS010000373         1595 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_62736_length_1595_cov_2.537013, whole genome shotgun sequence.
ACCESSION   DAOTKS010000373 DAOTKS010000000
VERSION     DAOTKS010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62736_length_1595_cov_2.537013"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="P5236_06625"
     CDS             complement(<1..328)
                     /locus_tag="P5236_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU64071.1"
                     /translation="MINSLKLSKSVFLLAALFFFYPANSKGFLSVKVQRIVDESGHEF
                     LLRGMGLGGWMLQEGYMLQMPGGGNQHQIKAKITDLIGDEACERFYEKWLSNHVTKTD
                     IDSLAKW"
     gene            complement(405..>1595)
                     /locus_tag="P5236_06630"
     CDS             complement(405..>1595)
                     /locus_tag="P5236_06630"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HRU64072.1"
                     /translation="RATWINKDNDPNTIWQSIYYDDPQAKTYLQAITERGAQDGLNVY
                     QDDASNPKVAVVVIGEKSYTHGTEWADKNPNIPDDQLSIIQNFHNRGIKVVTVILMPR
                     PYVLTPLLELSNAILVVYRGGTGIGQATAACIFGDYAPSGKLPFQLPKSDTQIGTDNL
                     NNMVEKWDLPYDIGATDAERILIRNYINQDLTVPPIFGDPLFQYGFGLQGFGNSDDSS
                     PLAFDLLSPSNNSIFQSNQITFSWQESSDPESSIAYYEVYIDDVKRAEVTSPFYTTIV
                     SDCGIHSWYVVAVNGSGLRKNSTSTYQFTISPSGLATAGMNPEVSVFPNPSNGHFVIK
                     LNNFVEKYYIKITDMTGKIIYVNDSPSSINELDLTKYPQGVYFMCIECNKNQFRKVIK
                     IIKI"
BASE COUNT          526 a          321 c          234 g          514 t
ORIGIN      
        1 cccattttgc taaagaatca atgtcagttt ttgtaacatg attgcttaac catttttcat
       61 aaaatctttc acacgcttcg tccccaatca aatcggttat ttttgctttt atctgatgct
      121 gattaccacc tcctggcatt tgaagcatgt aaccttcctg aagcatccag ccaccaagtc
      181 ccattcctcg taacaaaaat tcatgaccag attcatccac aattcgctgc acttttacac
      241 ttaaaaaacc ctttgaattt gctggataga agaagaaaag agcagcaagt aaaaaaaccg
      301 acttagataa ttttaagcta tttatcatca caaaattttt taaaaaaatt attcactctt
      361 ttgaaactat ttttctgaga actgatagct cctcatatgt tttcctatat ttttataatt
      421 ttgatcacct tcctgaattg gtttttatta cattcaatgc acataaagta tactccctgc
      481 ggatatttag taaggtctaa ctcattaata gaagaaggag aatcatttac ataaataatt
      541 tttcctgtca tatcggtgat tttgatataa tatttttcta cgaaattatt caacttaata
      601 acaaaatgtc catttgaagg attgggaaaa acagatactt caggattcat ccctgcagtc
      661 gccaacccag aaggagaaat agtaaactgg taagttgatg tagaattttt ccttagccca
      721 cttccattaa ctgccacaac ataccaacta tggattccac aatcagaaac aatagtagta
      781 taaaaaggag acgttacctc agcccgcttc acatcatcta tataaacttc ataataagca
      841 atagaagatt caggatcaga actctcctgc catgaaaaag ttatctgatt cgattgaaaa
      901 atactattat tcgacggaga aagcaaatca aaagctaaag gtgaactatc atcactattt
      961 ccaaatccct gcaaaccaaa accatactgg aagagcggat ctccaaaaat aggaggaacc
     1021 gttaaatctt gattgatata atttcttatt aaaatacgct ctgcatccgt tgctccaatg
     1081 tcataaggta aatcccattt ctcaaccata ttgttaagat tatcagtacc tatttgagta
     1141 tccgactttg gaagctgaaa aggtaacttc ccacttggag cataatcgcc aaaaatacat
     1201 gctgccgttg cttgccctat accagtaccg ccccgataaa ctactaaaat tgcattggat
     1261 agttctaata atggagttaa tacatacgga cgtggcatta aaataacagt aacaactttt
     1321 attcctctat tatgaaaatt ctgaattatg gataattgat cgtctggaat atttggattt
     1381 ttatctgccc attctgtacc atgggtataa ctcttttctc ctataacaac cacagcaact
     1441 tttggatttg atgcatcatc ttgataaaca tttaagccat cttgtgctcc acgttcggtg
     1501 atagcctgta aatatgtctt ggcttgagga tcatcatagt aaatcgattg ccaaatagtg
     1561 tttggatcat tatctttatt tatccatgtt gctcg
//
LOCUS       DAOTKS010000374         1588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_63099_length_1588_cov_3.866928, whole genome shotgun sequence.
ACCESSION   DAOTKS010000374 DAOTKS010000000
VERSION     DAOTKS010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63099_length_1588_cov_3.866928"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="P5236_06635"
     CDS             <1..783
                     /locus_tag="P5236_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HRU64073.1"
                     /translation="TNLREDKVYSLSKLVATYSGIRIPHNKPVIGENVFTQVAGIHAD
                     GDNKSNLYYNDLLPERFGRHREYALGKNSGKANILKNLQELGIELDEEMMRKVTERVI
                     ELGDKKQFVTMDDLPYIVSDVLNSEPEEQSIKLLNYSLSLAYGLRPVATVKMQINGEV
                     FEQTAAGEGQYNAVSKAMWKIYKNLNKPTPELLDYEVVIPPGGQTNAIVQTIITWKFN
                     DKVFKTRGLDGDQTVAAIKATMRMLNLIEHGHIHKSDYENLP"
     gene            862..>1588
                     /gene="leuB"
                     /locus_tag="P5236_06640"
     CDS             862..>1588
                     /gene="leuB"
                     /locus_tag="P5236_06640"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HRU64074.1"
                     /translation="MGKNINLAVLPGDGIGPEIIDQALKVTEAICNKFGHILTYEFGM
                     VGACAIDEVGEPYPQATHELCMQSDAVLFGAIGSPKYDNNPSAKVRPEQGLLKMRKEL
                     GLYANIRPVTTFASLIEKSPLKTSLIEGADFICIRELTGGIYFGRPQGRSEDGNTAYD
                     TCIYTREEIERVVELAYKFASQRRKKITMVDKANVLATSRLWRQITQEISAKYPQIDT
                     EYLFVDNAAMRIIQNPKNFDVIVT"
BASE COUNT          564 a          232 c          326 g          466 t
ORIGIN      
        1 acaaatcttc gtgaagacaa agtttattcg ctcagcaaat tggtagcaac ttattcagga
       61 ataagaatac ctcataacaa gccggtaatt ggtgagaacg tattcacaca agtagctggc
      121 attcatgcag atggagataa taaaagtaat ttatattata atgatttatt acccgaacgt
      181 tttggacgtc accgagaata tgctttagga aagaattcag gaaaagcaaa tattttgaaa
      241 aatttacaag aattgggtat tgagcttgat gaggaaatga tgagaaaagt gacagagcgc
      301 gtcattgaat taggtgataa aaaacaattt gtcacaatgg acgatttgcc ttacatagtc
      361 tctgatgttt tgaacagtga gccagaagaa caatcaataa aattgttaaa ttattcgtta
      421 tcattggctt atggacttcg accggtggca accgttaaga tgcaaattaa tggagaagtt
      481 tttgaacaaa cagccgctgg cgaagggcaa tacaatgccg tttcaaaggc aatgtggaaa
      541 atttataaaa atttgaataa acctacccct gaattactcg attacgaagt tgtaatccct
      601 cctggtggac aaaccaatgc gattgttcaa actattatta cctggaaatt taatgataaa
      661 gttttcaaaa ccagaggatt agacggagac caaactgttg cagccataaa agcgacaatg
      721 cgaatgttga atttgattga acatggtcat attcataaaa gtgattatga aaatttgccg
      781 taataaacat gtttttagga aaaataattt atctttaatg tcgaatattt attggtaata
      841 aaaacgtaaa aaataataac aatgggaaag aatattaatt tagcagtttt gcctggtgat
      901 ggaattggac ccgaaattat tgatcaggct ttgaaagtga cagaggctat ttgtaataaa
      961 tttggacata tattaactta tgaatttgga atggttggtg cttgtgccat agatgaagta
     1021 ggcgaacctt atccgcaagc tactcatgaa ttgtgcatgc aatctgatgc ggtacttttt
     1081 ggagcaattg gaagtcctaa atatgacaat aatccatcgg caaaagttcg tcctgaacaa
     1141 ggtttactta aaatgcgaaa ggagttgggt ttatatgcca acattcgtcc agtaacaacc
     1201 tttgcttcat taatcgaaaa atcaccgttg aaaacttcgt tgatagaagg agctgatttt
     1261 atttgtatta gagaactgac gggtggaata tatttcgggc gtcctcaagg aagaagtgag
     1321 gatggaaata cagcttacga tacttgtatt tatactcgcg aagaaattga aagagtggtg
     1381 gaattggctt ataaatttgc cagtcaacgt agaaaaaaaa taacaatggt tgataaggca
     1441 aatgttttgg ctacttctcg cttatggaga cagattactc aagaaatttc agcaaaatat
     1501 ccacaaatag atacagaata cctttttgtg gacaatgctg cgatgcgtat tatacaaaat
     1561 cccaaaaatt ttgatgtcat tgtaactg
//
LOCUS       DAOTKS010000375         1587 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_63154_length_1587_cov_4.809399, whole genome shotgun sequence.
ACCESSION   DAOTKS010000375 DAOTKS010000000
VERSION     DAOTKS010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63154_length_1587_cov_4.809399"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="P5236_06645"
     CDS             <1..691
                     /locus_tag="P5236_06645"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRU64075.1"
                     /translation="ESAKRGGELGFLGRGQLVPEFAEVAFNLQDPKKVSRIVETEFGF
                     HIIQLIEKRGDRINCRHILLKPHISEEDKQKAFHTMDSIANLIRNQKMTFEQAVIYYS
                     HDKNTAMNAGLMLNEKTGTSKFEYQDLPSEIAKIVYNMKVGEISDPFLMIDPKTNKEV
                     VAIVKVKSKIENHKANLRDDYLLIKNYYENKKRDEFLRNWIAKKQKETYIRIDPAWQN
                     CEFQYPGWIKK"
     gene            780..>1587
                     /locus_tag="P5236_06650"
     CDS             780..>1587
                     /locus_tag="P5236_06650"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HRU64076.1"
                     /translation="MYQYFVILFLFFIPFLALQAQNKPKQLKEEILKAQKESEKPPKV
                     KKKELTKPTTTLPPLKPVAPIANPLENKNATLIYLENSEIASFDKFQNPDIQVLKGNV
                     IFRHENALMYCDSAYFYPNSNSLDAFGNIRMVQGDTIFIYGNFLYYDGNTKLARLRKN
                     VRMENRKTVLTTDSMNYDRMMNLAYYYTGGKIQDELNTLTSIWGQYSPVTNEAIFKNK
                     VHLENENFIMDADTLKYNTKTNIANIVGKTHVIYKNETDIYSTRGWYNTAN"
BASE COUNT          607 a          239 c          256 g          485 t
ORIGIN      
        1 agaaagtgca aaacgtggag gtgaattagg cttcctggga cgaggacaat tggtgcctga
       61 atttgcagaa gtagccttca atctccaaga tcccaaaaaa gtgtcacgaa ttgtagaaac
      121 tgaatttgga tttcatatta ttcaattgat tgaaaaacga ggcgaccgta ttaattgtcg
      181 gcatatcctg ctaaaaccac acatttctga agaagataaa cagaaagctt ttcatacaat
      241 ggattcaatt gccaatttga ttcgtaatca aaaaatgact tttgaacaag ctgtcattta
      301 ttattctcac gataaaaata cagcaatgaa tgcaggtttg atgttgaatg agaaaacagg
      361 cacttcaaaa tttgaatatc aagatttacc ttccgaaatt gcaaagattg tttacaatat
      421 gaaagtgggt gaaatttcag atcctttttt aatgattgat cctaaaacca ataaagaagt
      481 tgttgccatt gtaaaagtca aatcaaaaat tgaaaatcat aaagccaatt tacgtgacga
      541 ttatttattg ataaaaaatt attacgaaaa caaaaaaaga gacgagtttt tgcgaaactg
      601 gattgctaaa aaacaaaagg aaacttatat tagaattgat ccagcatggc aaaactgtga
      661 attccaatat ccaggttgga taaaaaaatg atttatcctg ccaaattcaa taattttaca
      721 tgatttcgtc catttttaaa aaaatattta attcttgttt tttagctaac aaaagaattg
      781 tgtatcaata ctttgttatt ctttttttgt tttttattcc ttttctcgct ttacaagctc
      841 aaaataagcc taaacaatta aaagaagaaa ttttaaaagc acaaaaagaa tcagaaaaac
      901 caccaaaagt caaaaagaaa gaattaacca agcctacaac aaccttgccc ccattaaagc
      961 cagtagctcc gatagcaaac cctctggaaa ataaaaatgc tacgttaatt tatctggaaa
     1021 attctgaaat agccagtttt gataaatttc agaatcctga tattcaggtt ttaaaaggaa
     1081 atgttatttt tcgacatgaa aacgcattga tgtattgcga tagtgcctat ttttatccca
     1141 attcaaattc attggatgct tttggtaata ttcgcatggt gcaaggtgat actatattta
     1201 tatatggtaa tttcttatat tacgatggaa atactaaact tgcgcgttta agaaaaaatg
     1261 ttcgcatgga aaatcggaaa acagttttga ctactgatag tatgaattat gatcggatga
     1321 tgaatttggc ttattattat accggcggaa aaattcagga tgaattaaat actttaactt
     1381 ctatttgggg acaatattcg cctgtcacca atgaagccat cttcaaaaac aaagtacatc
     1441 tggaaaatga aaattttata atggatgctg atactttaaa atacaatacc aaaacaaata
     1501 ttgccaatat agtaggtaaa acgcacgtaa tctacaagaa tgagacagat atttattcaa
     1561 ccagaggatg gtataataca gcaaatg
//
LOCUS       DAOTKS010000376         1576 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_63782_length_1576_cov_3.499014, whole genome shotgun sequence.
ACCESSION   DAOTKS010000376 DAOTKS010000000
VERSION     DAOTKS010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63782_length_1576_cov_3.499014"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1576)
                     /locus_tag="P5236_06655"
     CDS             complement(<1..>1576)
                     /locus_tag="P5236_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRU64077.1"
                     /translation="FSPFYNDALFFSLGGQHGDYASQWLTSYIGVPGTLMVIIGTLLI
                     YCIISFNSALPFLKQIFSKKQKKQLSPDQNINVAIGTEIVPEEWLSKGVDESNEYFSG
                     QEISDVPFEIYPVETTDENKPLLEEEDRVREEENADPNYNFEKLGKYDPTLELAHYYF
                     PTLDLLKVYGTDNNAPIDMDEQIANKEKIISTLQNFDIEIESIKATVGPTITLYEIVP
                     KAGVRISKIRNLESDIMLSLAAIGIRIIAPIPGKGTIGIEVPNNDPQIVSMHSVIASK
                     KFQESKYELPIAFGRTITNEIFMFDLVKMPHLLVAGATGQGKSVGLNAIITSLLYKKH
                     PAELKLVLIDPKKVEFNIYSGIAKHFLAKLPDDSDAIVTDTSKVVETLQSLCTEMDNR
                     YDLLKKAHVRNIKEYNEKFIMRNLNPEKGHKYLPYIVVIIDEFGDLIMTAGKEVEMPI
                     ARIAQLARAVGIHMVIATQRPSVNIITGVIKANFPARVAFRVSSMMDSRTILDMPGAN
                     QLVGRGDMLFSQGNDLI"
BASE COUNT          484 a          287 c          278 g          527 t
ORIGIN      
        1 aatcagatcg tttccttgcg aaaaaagcat atcaccacga cctaccagtt gatttgctcc
       61 tggcatatcc aggatggtac gtgaatccat catggacgaa acgcgaaatg caactcgtgc
      121 tggaaaattt gccttaataa cacccgtaat aatgtttaca gaaggccgtt gtgtggcaat
      181 taccatgtga atgcctacag ctctggcaag ttgggcaatt cgagcaattg gcatttctac
      241 ttctttgcca gcagtcatga tcagatcgcc aaactcatca ataatcacca caatgtaagg
      301 taaatattta tgtccttttt caggattcaa attacgcatt ataaattttt cgttatattc
      361 tttaatgttt ctgacatggg cttttttaag taaatcataa cgattatcca tttcagtaca
      421 aagtgattga agtgtttcta ccactttact tgtatcagtg acaatggcat cgctgtcatc
      481 gggaagtttg gcaagaaaat gttttgcaat tccgctgtat atattgaatt ctactttttt
      541 gggatcaatc aaaaccaatt tcaattcagc aggatgtttt ttataaagca atgaagtaat
      601 aatggcattt aaacctactg attttccttg tccggtagca ccagctacca gcaaatgggg
      661 catttttacc agatcaaaca taaagatttc attggtgatg gttcgcccaa aagcaatagg
      721 taattcatat tttgattctt gaaatttttt tgaagcaata acagaatgca tggatacaat
      781 ttgaggatca ttattgggaa cttctatacc aatagtacct ttccctggaa tgggagcaat
      841 aattctaata ccaattgcag caaggctcaa cataatatcc gattcgaggt tacgaatttt
      901 tgaaatacgg actcctgctt ttggtacaat ttcgtaaagt gtaatggtag gaccaacggt
      961 tgcctttatg gattctatct caatgtcaaa attttgtaag gtactgataa ttttttcttt
     1021 attggcaatt tgttcatcca tatcgatggg tgcattgtta tcggtaccgt aaactttaag
     1081 caaatcgagt gttggaaaat aatagtgagc taattccagt gtaggatcat atttacctaa
     1141 tttttcaaaa ttataattgg gatcagcatt ttcttcttcc ctaactcgat cttcttcttc
     1201 aaggagaggc ttgttttcgt cagttgtttc aactggatat atttcgaatg gaacgtcact
     1261 tatctcttgt ccgctaaaat actcattact ttcatctact ccttttgaca accactcttc
     1321 aggaactatt tctgtgccaa tagcaacatt tatattttgg tcaggagaaa gctgtttttt
     1381 ttgttttttt gaaaaaattt gtttaagaaa aggaagagca ctattgaacg aaattataca
     1441 atatattagc aaagtcccaa tgattaccat taatgttcct ggtactccaa tataggaagt
     1501 caaccattga ctggcataat cgccatgctg accacccaat gagaaaaaaa gtgcatcgtt
     1561 ataaaaaggt gaaaaa
//
LOCUS       DAOTKS010000377         1572 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_64002_length_1572_cov_14.399473, whole genome shotgun
            sequence.
ACCESSION   DAOTKS010000377 DAOTKS010000000
VERSION     DAOTKS010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64002_length_1572_cov_14.399473"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            287..1201
                     /locus_tag="P5236_06660"
     CDS             287..1201
                     /locus_tag="P5236_06660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRU64078.1"
                     /translation="MKILFVCSKNSGKVASFIVEQAEAVQQAGLEVDFFTIEGKGWKG
                     YLSNRPLLIKKIKEFKPNVIHAHYGLSGLLANTQRKVPVITTFHGSDINDPEVFRFSK
                     WAIRLSAWNIFVSEKNIQAAKVKHKYSLIPCGVNTELFKPVDKHIARKKFGFSTGEKL
                     ILFAGSFDNPVKNSALALQAINFLPEVRLLELKNYSREEVALLMNAVDVCLMTSLNEG
                     SPQFIKEAMACNCPIVSVDVGDVKEQIEDVENCFITEARPEKIAEKIDTIFTNRSKRT
                     NGRERLMKSELEQSVVAKQLIKIYRKYA"
     gene            1194..>1572
                     /locus_tag="P5236_06665"
     CDS             1194..>1572
                     /locus_tag="P5236_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRU64079.1"
                     /translation="MLKLLQANEINLTEWQQLVENSAVASFFQTPECYQFYTSLSFLK
                     PFLYGVAEDGKLKGIACGYVQAEGGKLKQFFSRRAIIPGGILLDKNIEPDTLVALLEY
                     LKNELRKKAIYIECRNFNDYSEYR"
BASE COUNT          553 a          254 c          320 g          445 t
ORIGIN      
        1 acaatgaacc ttagtaacca ataataagcc catatcaacc agaccttatt accttatttc
       61 gataataatg aaaaacagaa aataagaatg ataacgaaat aatttttgca taagatgaat
      121 cattatgaaa aagctggagc tttatgtcta aaccgacgta gcgaaacgct acgccgaacg
      181 caaaaaagga caagataaaa agtattaagt agtagtaaaa tttcttaaat tttataaatt
      241 accattgact tatagcttga atctattaac agagttattg ataaacatga aaatattatt
      301 tgtttgcagt aaaaactcgg ggaaagttgc atcctttatt gtggaacaag cagaagcagt
      361 tcagcaggca ggtttggaag tggatttctt cactatcgaa ggaaagggat ggaaaggtta
      421 tttaagcaat cgacctttgt tgataaaaaa aataaaagaa tttaaaccga atgtaattca
      481 tgcccattac ggtttgtcgg gattactggc caatacccaa cgaaaagtgc ctgtgataac
      541 cacttttcat ggcagcgata tcaacgatcc ggaagtattt cgattttcaa agtgggctat
      601 tcgtttatcg gcgtggaaca tttttgtgtc agaaaaaaat attcaggcgg caaaagtaaa
      661 acataaatat tccttgattc cttgtggcgt gaatacggaa ctttttaaac ctgttgataa
      721 acatattgcc cgtaaaaaat tcggattttc gactggcgaa aagctgatac tttttgccgg
      781 aagctttgat aatccggtaa aaaattctgc attggctttg caagctatca actttttgcc
      841 ggaagttcgt ttgctggaat taaaaaacta cagccgtgaa gaagtagctt tattaatgaa
      901 tgcagtggat gtttgtctga tgaccagcct taatgaaggt tcgcctcaat tcatcaagga
      961 agcaatggca tgtaactgtc ccatagtcag tgtggatgtg ggtgatgtaa aagaacaaat
     1021 cgaagacgta gaaaattgtt ttattacaga agctcgtcct gaaaaaatcg ctgaaaaaat
     1081 agacactatt tttaccaata gaagtaaaag aaccaatgga agagaaagac taatgaaaag
     1141 tgagctggaa caatctgttg tggcaaaaca attaataaaa atttacagga agtatgctta
     1201 agctgctgca agcaaatgaa ataaatttaa ccgaatggca acaattggta gaaaattcag
     1261 cggttgcttc ctttttccaa acaccggagt gttatcagtt ttacacttcc ctatcctttc
     1321 tgaaaccttt tctgtatggt gtggctgagg atggaaaact gaaaggaatt gcttgcggat
     1381 atgtgcaggc agaaggcgga aaattaaagc agtttttcag ccggcgagcc attattcccg
     1441 gaggaatttt actggacaag aatattgaac ctgacacttt ggtggctttg ctcgaatatc
     1501 tgaaaaacga acttcgcaaa aaagccattt atattgaatg caggaatttt aacgattaca
     1561 gcgaatatcg tc
//
LOCUS       DAOTKS010000378         1564 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_64470_length_1564_cov_4.051027, whole genome shotgun sequence.
ACCESSION   DAOTKS010000378 DAOTKS010000000
VERSION     DAOTKS010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64470_length_1564_cov_4.051027"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="P5236_06670"
     CDS             <1..633
                     /locus_tag="P5236_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRU64080.1"
                     /translation="GRSEDGNTAYDTCIYTREEIERVVELAYKFAAQRRKKITMVDKA
                     NVLATSRLWRQITQEISVKYPQIDTEYLFVDNAAMRIIQNPKNFDVIVTENLFGDILT
                     DEASVITGSLGMLPSASMGIHTSLFEPVHGSYPQATGKNIANPMATILSAALMFEYAF
                     NLKEESNLIREAVDACITAGVVTEDIASGEKAYKTSEVGEWITNYILKKK"
     gene            699..>1564
                     /locus_tag="P5236_06675"
     CDS             699..>1564
                     /locus_tag="P5236_06675"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRU64081.1"
                     /translation="MFNNYVGKLTDNKKKALLLIFLFGIISFLGDIIYEGARSVNGPY
                     LKTLGANAANVGIVVGIGELLGYAIRLFSGYFSDKKNAAWFFTILGYSLLVSIPLLSF
                     SNFWQLAAMFIVIERIGKGLRSPAKDTLLSYATKQVGTGIGFGISEFLDQLGATLGPL
                     IFMIFFLTTGNSQNTLIDYQHAYSLLWIPFVLLLIVVLFAYFKVKTPETLESSENQPT
                     GFTKVFWFYSLFTFITTFGFINFAIIGFHLKTKAILPDSQIPLLYTIAMVIDAIFALI
                     VGKIYDKYKALH"
BASE COUNT          492 a          224 c          289 g          559 t
ORIGIN      
        1 ggaagaagcg aagatgggaa tactgcttat gacacctgta tttatactcg agaagaaatt
       61 gaaagagtgg tagaattggc ttataaattt gctgctcagc gccgaaaaaa aataacgatg
      121 gtagataagg caaatgttct cgcaacttct cgtttatgga gacagattac tcaagaaatt
      181 tcagtgaaat atccacaaat agatacagaa tatctttttg tggataatgc tgcgatgcgt
      241 attatacaaa atcccaaaaa ttttgatgtc attgtaactg aaaatctttt cggcgacatt
      301 ctcacagatg aagcttcggt aattacaggt tcattaggaa tgttaccttc tgcttcgatg
      361 ggtattcata cttcactttt tgaacctgta catggttctt atccgcaagc tacggggaaa
      421 aacatagcca atccaatggc aactatttta tcagcagctt tgatgtttga atatgctttt
      481 aatttaaagg aagaaagtaa tttaattcgt gaagctgtcg atgcttgtat cacagcgggc
      541 gtagtaaccg aagatattgc ttccggagaa aaagcttaca aaacttccga agtaggagaa
      601 tggattacta attatattct taagaaaaaa taaaaaaatt tttcagaggt tattattaaa
      661 atgaaaaatt aacaatcaaa gttagaattt attgaagcat gttcaataat tatgttggca
      721 aacttactga taataaaaag aaagctcttc ttttaatatt tctttttggt atcattagct
      781 ttttgggtga cattatttat gaaggtgcaa gaagtgtcaa tggaccttat ttaaaaacct
      841 taggagctaa tgctgctaat gtgggaatag ttgtaggcat tggtgaattg ttgggttatg
      901 ctattcgttt attttcaggt tatttctcag ataaaaagaa tgctgcctgg ttttttacca
      961 ttttaggata tagtttatta gtttctattc ctttattatc tttttccaat ttctggcaac
     1021 tcgctgctat gttcattgtg attgaaagga ttggaaaagg cttgagaagt ccagcaaagg
     1081 ataccttgtt atcttatgct accaaacaag tagggacggg gattggattt ggaatttcag
     1141 aatttctcga tcaattggga gccactttag gtccattaat ttttatgatt ttctttttga
     1201 ctacgggcaa tagtcaaaat actttgattg attatcaaca tgcttacagc ttgttatgga
     1261 ttccttttgt gcttttatta attgtagttt tgtttgcata ttttaaagta aaaacgccgg
     1321 aaacacttga aagttctgaa aatcaaccaa ctggatttac aaaagtattc tggttttatt
     1381 ctttgttcac ttttattact acttttggct ttatcaattt tgctattatt ggatttcatc
     1441 tgaaaacaaa agcaatttta cctgatagtc aaattccttt actttatact attgcaatgg
     1501 taattgatgc catttttgct ttgattgttg ggaaaatcta tgataaatat aaagctttgc
     1561 atca
//
LOCUS       DAOTKS010000379         1562 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_64590_length_1562_cov_5.739217, whole genome shotgun sequence.
ACCESSION   DAOTKS010000379 DAOTKS010000000
VERSION     DAOTKS010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64590_length_1562_cov_5.739217"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..1099
                     /gene="prfA"
                     /locus_tag="P5236_06680"
     CDS             14..1099
                     /gene="prfA"
                     /locus_tag="P5236_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRU64082.1"
                     /translation="MANNSLLEKLEGLQNKFEEVSILINDPAVLGDQKKFTKLNKEYH
                     DLEQILSVKKEYEIALTNLAEAKKILENETDADLREIAKMEIEQLEKLIPDLEEKIKL
                     LLIPADPADKKNAIVEIRSGTGGDEAALFAGDLFHMYAKYCENKGWKIEITDFNEGTA
                     GGYKQIVFTIRGENVYGTLKYESGVHRVQRVPQTETQGRIHTSAATVAVFPEAEEFDI
                     ELKESDIRVDIFCSSGPGGQSVNTTYSAIRLTHIPTGITVQCQDEKSQHKNKLKAMTE
                     LRSRLYAIEYQKYLDEISSKRKTMVSTGDRSAKIRTYNFPQGRVTDHRINYTLYNLPA
                     FMNGEIQPLIDQLIIAENTERLKAAEL"
     gene            1137..>1562
                     /gene="pyrF"
                     /locus_tag="P5236_06685"
     CDS             1137..>1562
                     /gene="pyrF"
                     /locus_tag="P5236_06685"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005843024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRU64083.1"
                     /translation="MTKHELFEQIKKKKSFLCIGLDTDIHKIPSHLLKSSEDPIFDFN
                     RAIIDATAKFCVAYKPNLAFYESAGTKGWESLARTVNYLKNNYPEQFLIADAKRGDIG
                     NTSQKYAETFFENLNFDAITLHPYMGKDTVVPFLNYKNKW"
BASE COUNT          568 a          242 c          285 g          467 t
ORIGIN      
        1 aaaaaacaag aagatggcta ataattcttt attggaaaaa ctggaaggac tacaaaataa
       61 atttgaggaa gtttcaattt taataaatga tcctgcggtg ttaggggatc aaaaaaaatt
      121 cacaaaactt aataaggaat accatgactt agagcagatt ctttccgtaa aaaaagaata
      181 tgaaatagct ttaactaatt tggctgaagc aaaaaaaata cttgaaaacg aaactgatgc
      241 cgacttacgt gaaatagcta aaatggaaat tgaacagtta gaaaaattaa ttcctgattt
      301 ggaagaaaaa ataaaattgc ttctcattcc tgccgatcca gcagataaga aaaatgccat
      361 tgtagaaata cggagtggga ctggagggga tgaagctgct ctttttgctg gtgatttgtt
      421 tcatatgtat gcaaaatatt gtgaaaacaa aggttggaaa atagaaatta ccgattttaa
      481 tgaaggaaca gcgggtggtt acaaacaaat tgtttttact ataaggggcg aaaatgtgta
      541 tggaacgctt aagtatgagt caggtgttca tcgcgtgcaa cgtgttccac aaacggaaac
      601 tcaaggacga attcatactt cggcagctac tgtagcggtg tttcctgaag ctgaagaatt
      661 cgatattgaa ttgaaagaat cggatatccg tgtagatatt ttttgttcct ccggtccagg
      721 tggacagtcg gtcaatacaa cctattctgc cattcgtctg acacatattc caacgggtat
      781 taccgttcaa tgtcaagatg aaaaatccca gcataaaaat aaattgaaag caatgacaga
      841 attacgttca cgcttgtacg ctattgaata tcaaaagtat ctggacgaaa ttagttcgaa
      901 acgtaaaaca atggtatcta ctggtgatcg ttctgcaaaa attcgtacct ataattttcc
      961 acaaggacga gttaccgacc atcggattaa ttatacactt tataatcttc cagcttttat
     1021 gaatggtgaa attcaaccgc tgattgatca acttatcatt gccgaaaata ctgaaagatt
     1081 aaaagctgct gaattataat tatttttttc attaccatga ttttaaaaat aattttatga
     1141 ctaagcatga actttttgag cagataaaaa agaaaaaatc atttttatgc attggacttg
     1201 atactgatat tcacaaaata ccttctcatc tattaaaatc atcagaagat ccaatttttg
     1261 attttaaccg agccattatt gatgctacag caaagttttg tgtggcttat aaaccaaact
     1321 tagcattcta tgagagtgct ggaacaaaag gttgggaatc tttagcccga acagtaaatt
     1381 atttgaaaaa caattatcca gaacagtttc ttattgccga tgccaaacga ggagatattg
     1441 gaaacacatc tcaaaaatat gcagaaactt tttttgaaaa tttgaatttt gatgctatta
     1501 ccttacatcc ttacatggga aaagatacag ttgttccttt tttgaattat aaaaacaaat
     1561 gg
//
LOCUS       DAOTKS010000380         1559 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_64783_length_1559_cov_4.186170, whole genome shotgun sequence.
ACCESSION   DAOTKS010000380 DAOTKS010000000
VERSION     DAOTKS010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64783_length_1559_cov_4.186170"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="P5236_06690"
     CDS             complement(<1..323)
                     /locus_tag="P5236_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRU64084.1"
                     /translation="MSEKVRCLIIGSGPAGCTAAIYAARANLSPVLYQGLEPGGQLTL
                     TNEIENFPGFPEGISGAELMDKMQKQAEKFGAAVRFGVVTAVDFSLRPYKVIVDNEKA
                     IEAET"
     gene            complement(385..1020)
                     /locus_tag="P5236_06695"
     CDS             complement(385..1020)
                     /locus_tag="P5236_06695"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HRU64085.1"
                     /translation="MKQLFFSITALIISINSLFAQKSADAEKIINDLFYLFTNNAISS
                     NFKLTISEKNNINSQTYNGSFFLKGNKFMIDMNEMKVWYDGKTQWAYSQQNNEVTITE
                     PTAEELINTNPITFLSAYKNKFSAQFCKKSYSELYCIEMFPKAKNESILKVELKVNRT
                     TGDPVSLEINEKNGSTSLLTLINFKKNIKINDDFFVFHPSKYNDVYINDLR"
     gene            complement(1075..>1559)
                     /locus_tag="P5236_06700"
     CDS             complement(1075..>1559)
                     /locus_tag="P5236_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRU64086.1"
                     /translation="AFRVSSMMDSRTILDMPGANQLVGRGDMLFSQGNDLIRIQCAFV
                     DTPEVENIVNYISKQQSYPTAYYLPEYIKIEGDVSMGSIDMSKRDPLFEEAARLIVAN
                     QQGSTSLIQRKFSIGYNRAGRIIDQLEVAGIIGPYEGSKARQVLVADEYQLEQVLKHL
                     "
BASE COUNT          510 a          268 c          243 g          538 t
ORIGIN      
        1 atagtttcag cttcaatggc tttttcatta tcaacaataa ctttataagg tcttaaagaa
       61 aaatcgacag cagttaccac tccaaatctc acagcagcac caaatttttc tgcctgtttt
      121 tgcatcttat ccataagctc tgctccactg attccttcag ggaaaccggg aaaattttct
      181 atttcgttag taagagttaa ctgtccacct ggctctaaac cttgatataa tactggagag
      241 agattggctc gtgccgcata aatagctgca gtacatccgg ctggtcctga acctatgatc
      301 aaacaacgta ctttttcaga catttttatc agtaaatttt ttaaaaatat ttcttaaaga
      361 tgagctttaa tttattttga agatttatct caaatcgtta atataaacat cattatattt
      421 tgaaggatga aaaacaaaaa aatcatcatt gattttgatg ttttttttga aattaattaa
      481 tgtcaataga gaagttgaac cgtttttttc gtttatttct aaagaaactg gatcgcctgt
      541 ggttctatta acttttaatt ccactttgag aatggactca ttttttgctt tgggaaacat
      601 ttctatgcaa tataattccg aataggattt tttacaaaac tgagctgaaa atttgttttt
      661 ataagccgat aaaaaagtaa tcggattggt atttatcaat tcttcagcgg taggctctgt
      721 aattgtaact tcattatttt gctgggaata agcccattgc gtttttccat cataccacac
      781 tttcatttca ttcatatcaa tcatgaattt gttacctttt aaaaaaaagg atccgttata
      841 cgtttgagaa tttatattat ttttttcact aatagtaagt ttaaaatttg aagaaatggc
      901 attatttgta aaaagataaa ataaatcgtt tataattttc tcagcatcag cacttttttg
      961 agcaaaaaga ctgttgattg aaattatcaa tgctgtgatt gaaaaaaata attgtttcat
     1021 ttccagaggt tttaaaaaat tataacttga aaaagaggta ttgaaaagaa tttgttaaag
     1081 atgcttcaaa acttgttcca attgatattc atcggctact aagacttgac gggctttact
     1141 cccttcgtaa ggaccaatga ttcccgccac ttccaattga tcaataatac gtcctgctcg
     1201 attatatcca attgaaaatt tccgttgaat cagcgaagta gaaccttgtt gattggcaac
     1261 aattaagcga gcagcttctt caaacaaagg atctcgtttg ctcatatcaa tggagcccat
     1321 agaaacatct ccttctatct ttatgtattc tggcaaataa taagcggtag gataactttg
     1381 ctgtttgcta atataattaa caatattttc aacttcagga gtatcaacaa aagcacattg
     1441 gatacgaatc agatcgtttc cttgcgaaaa aagcatatca ccacgaccta ccagttgatt
     1501 cgctcctggc atgtcaagga tggtgcgtga atccatcata gacgaaacac gaaatgcaa
//
LOCUS       DAOTKS010000381         1558 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_64841_length_1558_cov_4.762475, whole genome shotgun sequence.
ACCESSION   DAOTKS010000381 DAOTKS010000000
VERSION     DAOTKS010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64841_length_1558_cov_4.762475"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /locus_tag="P5236_06705"
     CDS             <1..1352
                     /locus_tag="P5236_06705"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRU64087.1"
                     /translation="SSPGDFNSVVEISKTVTWPTICALTRAVEKDIEVAAEALKYAKR
                     KRIHTGIGTSDYHIKYKFNSTQEEILERSIAAVKYAKKFVEDVEFYCEDAGRTDNEYL
                     ARVVEAVIKAGATVVNIPDTTGYCLPWEYGEKIKYLMENVKGIQNAVISTHCHNDLGM
                     ATANTIMGIVNGARQAEVTINGIGERAGNTALEEVVMILKCHKSLNIETQINTKKIYP
                     TSRLVSNLMNMPIQPNKAIVGRNAFAHSSGIHQDGVLKNRENYEIMNPEDVGIDHNAI
                     VLTARSGRAALKHRLSTLGVELDNDKLDMVYEEFLKLADKKKVINDDDILMLAGKERH
                     EKNRIKLDYLQVTSGVGIKPIASIGLIIGNEKFEAAATGNGPVDAAINALKKIINRKM
                     TLQEFTIQAINKGSDDVGKVHMQIEHEGKIYYGFGANTDIVAASVEAYIDAINKFVE"
BASE COUNT          551 a          213 c          325 g          469 t
ORIGIN      
        1 tttcaagtcc aggtgatttt aattcggtag tggaaatttc aaaaactgtt acttggccaa
       61 caatttgtgc tttaacacgt gctgttgaaa aagatattga agtggctgca gaagcactaa
      121 aatacgcaaa aagaaaacgg attcatacag gtataggaac ttctgattat catatcaaat
      181 ataaatttaa ttctacacag gaagaaattt tagaacgttc aatagcagcg gtgaaatatg
      241 caaagaaatt tgttgaagat gtagaatttt attgtgaaga tgcaggaaga acagacaatg
      301 aatatctggc gcgtgtagta gaagcagtca ttaaagcagg tgctacagtg gtaaatatac
      361 ctgatacaac aggttattgt ttgccttggg aatatggaga aaagattaaa tatctgatgg
      421 aaaatgtaaa aggcattcag aatgccgtta tttctaccca ttgtcataat gatttgggta
      481 tggcaactgc caatactatt atgggaattg tgaatggtgc tcgtcaggcc gaagtaacca
      541 ttaatggcat tggcgaacgc gctggaaata ctgcccttga agaagtagtt atgattttaa
      601 aatgtcataa aagcttgaac atagaaacac aaattaatac caaaaagatt tatccaacca
      661 gccgattggt ttctaattta atgaatatgc ctattcagcc aaataaagca attgttggca
      721 gaaatgcttt tgcacattca tcgggaattc atcaggatgg agttttgaaa aatcgagaga
      781 attatgaaat tatgaatcct gaagatgtag ggattgatca caacgccatt gttttgacag
      841 ctcgtagcgg cagggcagct cttaaacatc gtcttagtac tttaggagta gagttggata
      901 atgataaact tgatatggtt tatgaagagt ttcttaaatt agctgataag aagaaagtaa
      961 ttaacgatga tgatattttg atgcttgccg gtaaagaacg ccatgaaaaa aatcgaatca
     1021 aactggatta tttgcaagtt acgtcgggtg taggcataaa acccatagcc agtattggtc
     1081 ttattatagg aaatgaaaaa tttgaagctg cagctactgg aaatggacct gttgatgcag
     1141 caattaatgc cctgaaaaaa attatcaata ggaaaatgac gctccaagaa tttactattc
     1201 aagctattaa taaaggtagc gatgatgtag ggaaagtgca catgcaaatt gaacatgaag
     1261 ggaaaattta ttatggtttt ggtgctaata ctgatattgt ggctgcttct gtagaagcct
     1321 acattgatgc cattaataaa tttgttgaat gattaggaaa ttatttctta ttgattgaat
     1381 tttagtttgg attaaaaaaa caacaaacaa attttttaaa agatatatga aaactttatt
     1441 tgataaaatt tgggatgatc atgtggtttc cacgatagaa aatggtccta ctcaattata
     1501 cattgacagg cattatgttc atgaagtaac aagtcctcag gcttttaatg gccttcgt
//
LOCUS       DAOTKS010000382         1557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_64916_length_1557_cov_2.918109, whole genome shotgun sequence.
ACCESSION   DAOTKS010000382 DAOTKS010000000
VERSION     DAOTKS010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64916_length_1557_cov_2.918109"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="P5236_06710"
     CDS             complement(<1..418)
                     /locus_tag="P5236_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxychorismate lyase"
                     /protein_id="HRU64088.1"
                     /translation="MLRFIETIKLLDGKFYRLEFHQNRIDKVFEDYFKEKAKIHLKEY
                     LYALPFPQKGLYKCRIIFDSRIQKLEYIPYKLRKICSLRLLETDIKTLAYKKENRSKL
                     NNAFAHRGNCDDVLLLKNGLVTDTSFCNVAFFDGNTW"
     gene            complement(402..1409)
                     /locus_tag="P5236_06715"
     CDS             complement(402..1409)
                     /locus_tag="P5236_06715"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HRU64089.1"
                     /translation="MTTALFPYQLYHRNELISQLNILGSLHIPFLFIIDFEGKNGYII
                     KSDELDDHFVKFDFSGENILKKENNNYLGKKIEWQIMPVSFEEYHSKFQFVMDQIHKG
                     NTYLVNLTQPTEIKTNVTLLDLYELGTSKYKLWLKDKFTVFSPEIFIKINNGKISTFP
                     MKGTINASVTDAEKIILNDPKENAEHATIVDLLRNDLSIKAINVEVKRHRYIDKLRTN
                     KNILLQVSSEITGILPENYHKMLGDILFSMLPAGSISGAPKNKTMEIIHQIEGYKRGF
                     YTGICGWFDGNSLDSAVMIRYIEQKGNKLIFKSGGGITFQSDSKKEYQELIEKVYAPI
                     Y"
BASE COUNT          493 a          254 c          237 g          573 t
ORIGIN      
        1 cccaagtatt gccatcaaaa aaagcaacat tgcaaaaaga ggtatccgtc actaaaccat
       61 tttttaacaa taaaacatcg tcgcaatttc ctctgtgggc aaacgcattg tttaatttgc
      121 ttcggttttc ttttttataa gccaatgttt taatatctgt ttccagcaac cgtaaagagc
      181 aaatttttcg caacttgtat gggatatatt cgagtttttg aattcttgaa tcaaatataa
      241 tcctgcattt atatagacct ttttgtggaa agggtagagc atataaatat tcctttagat
      301 gaatttttgc cttctccttg aaataatctt caaaaacctt gtctatacga ttttgatgaa
      361 actcaagccg ataaaacttg ccatcaagaa gttttatcgt ttcaataaat cggagcatat
      421 actttttcga taagttcttg atattctttt ttactgtcac tttgaaaagt gatgccacca
      481 ccacttttaa aaataagttt atttcctttt tgctcaatat aacgtatcat tacagccgag
      541 tcgagactat taccatcaaa ccaaccacaa atacctgtgt agaaaccacg tttatagcct
      601 tctatttgat gaatgatttc cattgttttg tttttgggag ctccgctaat tgagccagca
      661 ggaagcattg aaaaaaggat atctcctaac attttgtgat aattttcggg taatatacct
      721 gttatttctg aacttacttg cagtaatata tttttattgg tacgaagctt atcaatatat
      781 cgatggcgtt tcacttcaac atttatggct ttgatactta aatcattgcg aagtaaatcg
      841 acaatggtag catgctcggc attttctttg ggatcgttca gaattatttt ttcggcatct
      901 gttacagaag cattaatggt gcctttcatg gggaaagtag aaattttgcc gttatttatt
      961 ttgataaaaa tttcaggaga aaatacagta aatttgtctt ttagccaaag tttgtattta
     1021 gaagttccaa gctcatataa atcgagtaag gttacgtttg tttttatttc tgtcggctgc
     1081 gtcagattaa ccaaatatgt atttccttta tgaatttgat ccattacaaa ttgaaatttt
     1141 gaatggtatt cctcaaaact taccggcatt atttgccatt caattttttt acccaaatag
     1201 ttattgtttt ctttttttag aatattttca cctgagaaat cgaattttac aaaatgatcg
     1261 tctaattcat ccgattttat aatataacca ttttttcctt cgaaatcgat aataaacaag
     1321 aaagggatgt gcaaacttcc taagatattc aattgggaaa taagttcatt tcgatgataa
     1381 agttgataag ggaataatgc tgttgtcatt ttttaattaa aatgtcaatt tcaaataatt
     1441 tacaatcttt tatatttaaa aagagatata aaatgtgtaa ccaatgaata accccgaggc
     1501 aagcctcgag gaattctttt gattaaaaac tgtcaaacct ttggttttca gtcaact
//
LOCUS       DAOTKS010000383         1549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_65380_length_1549_cov_4.357430, whole genome shotgun sequence.
ACCESSION   DAOTKS010000383 DAOTKS010000000
VERSION     DAOTKS010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65380_length_1549_cov_4.357430"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..985)
                     /gene="prmC"
                     /locus_tag="P5236_06720"
     CDS             complement(143..985)
                     /gene="prmC"
                     /locus_tag="P5236_06720"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRU64090.1"
                     /translation="MEEIMKYIQTELKGYYPDTEIQSFGYLLLAKITGLSRSEILINK
                     NTKFSSEQWEKLNSFLQQLKEYVPIQYLLGETEFYGLPFMVNPSVLIPRPETEELVEW
                     IVKENDKNSALQILDIGTGSGCIAISLKHEFPFANVDACDISHEAIETARLNAKINHT
                     EIHFFEADILKNPLFDKKWDIIVSNPPYVPENEKETLLPNVLNYEPLLALFVPKDDPL
                     LFYRNIVDFAQIHLNQGGKLYFEIHYKAGENIVTLLEQEGFQQIELKKDLSGNDRMIR
                     AIYF"
     gene            1059..>1549
                     /gene="ribD"
                     /locus_tag="P5236_06725"
     CDS             1059..>1549
                     /gene="ribD"
                     /locus_tag="P5236_06725"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007841295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HRU64091.1"
                     /translation="MTEEEKYIARCLQIASYGDGYVAPNPLVGAVLVNEEKIIGEGFH
                     QYFGQAHAERNAIHSVKDPAQLKNATLYVNLEPCSHYGHTPPCANFIVDCKIPKVVIG
                     TYDPNPDIYGQGVEILQKAGIELKIGVLEKECKELNKRFFTFHEKKRPFVTLKWAQTI
                     DGF"
BASE COUNT          532 a          257 c          234 g          526 t
ORIGIN      
        1 gcttcttatc aattatgcat caattcttca gctcttggag tagaagaaac tgtcgcttat
       61 cttattgatt ttattaaaca aaaattacaa cttgactgac taattgtttt tgtactggtt
      121 tcgaaaaatt ttttcttcaa tgttaaaaat aaattgctct gatcattcta tcatttcctg
      181 acaaatcttt ttttaattca atctgctgaa agccttcttg ttcgagcaat gttactatat
      241 tttctcctgc tttgtaatga atttcaaaat aaagttttcc accctgattc aaatgaattt
      301 gagcaaaatc gacgatattc ctgtaaaaca acaaaggatc atctttaggt acaaacaaag
      361 ccaacaaagg ttcataattc aaaacatttg gcaaaagagt ttctttttca ttttctggaa
      421 cgtagggcgg attgctgaca ataatgtccc attttttatc aaaaagtgga ttttttaaaa
      481 tatctgcttc gaaaaaatga atttctgtat gattgatttt agcattcagg cgggctgttt
      541 cgattgcctc atgtgaaata tcacaagcat ctacgttggc aaaaggaaat tcatgcttca
      601 aactgatagc aatgcaacca cttcctgttc caatatctaa aatttgcaaa gctgaatttt
      661 tatcattttc ctttactatc cattcaacta attcttccgt ttcgggacga ggaatcaata
      721 ccgaaggatt aaccataaat ggcaaaccat aaaattctgt ttcgcctaaa agatactgaa
      781 ttggaacata ttctttcagt tgctgaagaa atgaatttaa tttttcccat tgttcagaag
      841 aaaattttgt atttttgtta attaaaattt cagaacgtga aagtcccgtt atttttgcta
      901 acaataaata accaaaactt tgaatctcag tgtcaggata gtaacctttt aattcagttt
      961 gaatatattt cattatttct tccataatgc aaaaataaac aatttgtaaa aacaagtaaa
     1021 taacaacatt atttttaaaa tcaggtttta ctggaaacat gactgaagaa gaaaaatata
     1081 tagcacgctg tttacaaata gcttcgtatg gcgacggtta tgtagctcct aatccattgg
     1141 taggagcagt tttggttaac gaagaaaaaa ttattggcga aggctttcac caatattttg
     1201 gtcaagctca tgcagaacga aatgccattc attcagtaaa agaccctgca caattaaaaa
     1261 atgctacttt atacgtaaac cttgaacctt gctctcatta tggacacaca cctccatgcg
     1321 ccaattttat tgtagattgt aagattccaa aagtagtgat tggcacctat gatcctaatc
     1381 cagatattta tggacaaggt gtagaaattc ttcaaaaagc agggattgaa ttaaaaatag
     1441 gcgttttaga aaaagaatgt aaagaattaa acaaacgttt ttttactttt catgaaaaga
     1501 aacgtccatt tgttacacta aaatgggctc aaactattga tggatttat
//
LOCUS       DAOTKS010000384         1548 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_65445_length_1548_cov_3.867381, whole genome shotgun sequence.
ACCESSION   DAOTKS010000384 DAOTKS010000000
VERSION     DAOTKS010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65445_length_1548_cov_3.867381"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1449)
                     /locus_tag="P5236_06730"
     CDS             complement(<1..1449)
                     /locus_tag="P5236_06730"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HRU64092.1"
                     /translation="MSTKGKVKGVVSNLVTVEIDGPVAQNEICYIELDGKMLMAEVLK
                     VIDTNAFVQVFESTRGLKVGNNVNFEEHMLEVTLAPGLLSKNFDGLQNDLNKFSGIFL
                     QRGEYNFPLDTEKLWEFKPLAKPGDEVEAGNWLGKVDENHQPHKIMVPFAFEGKYTVK
                     TIVPTGDYKILDTIAVLINEAGKEIEVNMTQKWPVKKAINIHTEKPRPFKLLETGVRV
                     IDTANPIVEGGTGFIPGAFGTGKTVLQHAISKQAEADIVIMAACGERANEVVELFVEF
                     PKLTDPHTDRLLMERTIIVANTSNMPVAAREASVYTAMTIAEYYRAMGLRVLLMADST
                     SRWAQALREMSNRMEELPGQDAFPMDLSAIIANFYSRAGYVQLKNGKTGSITFIGTVS
                     PAGGNLKEPVTESTKKVARCYFALEQARADSKRYPAINPIESYSKYFDYPEFEEYVAE
                     HISPDWTNMVNNMRTLLQRGREVQEQINILGDD"
BASE COUNT          462 a          322 c          223 g          541 t
ORIGIN      
        1 gtcgtctcct aagatattaa tttgttcctg cacttctctt cctcgttgaa gaagagttcg
       61 catattattg accatatttg tccaatcagg cgaaatatgc tcagcaacat attcttcaaa
      121 ttcaggataa tcaaaatatt tagaataact ttcaatagga ttaatagctg gatatcgttt
      181 actatctgcg cgtgcttgtt caagagcaaa ataacagcga gctacttttt tagtgctttc
      241 agtcaccggc tcttttaaat ttcctcccgc aggcgaaact gttccaataa aggtaataga
      301 accggtttta ccatttttta attgaacata tcctgcacgg ctgtaaaaat tagcaataat
      361 agcagataaa tccataggaa aagcatcttg cccaggcaat tcttccatcc gattactcat
      421 ctcacgcaaa gcctgagccc accgtgaagt agaatctgcc atcaataaaa ctcttaatcc
      481 cattgcccga taatactcag caatggtcat ggctgtgtaa acagaagctt cacgagcagc
      541 aaccggcata ttagaagtat ttgctacaat aatggttctt tccattaata gtctgtcggt
      601 atgtggatcg gttaattttg ggaattctac aaaaagttca acaacttcat tggctcgttc
      661 accacaagct gccataataa caatgtctgc ttctgcttgt ttggaaatgg catgttgtaa
      721 aacggttttt cctgtaccaa aagctcctgg aataaaacca gttccacctt caacaatggg
      781 attagcagta tcaattacac gtacgcccgt ttctaataat ttaaaaggac gtggtttttc
      841 ggtatgaata ttaattgcct tttttactgg ccatttttgt gtcatattta cctcaatctc
      901 tttcccagct tcatttatca atactgcaat agtatctaaa attttataat caccagttgg
      961 aacaatagtt ttaactgtat attttccttc aaatgcaaaa ggaaccataa ttttatgagg
     1021 ttgatgattt tcatctactt tgcctaacca attgcctgct tctacctcat caccaggttt
     1081 tgccaatggc ttgaattccc ataatttttc tgtatcaagt ggaaaattat attccccacg
     1141 ttgaagaaaa attcctgaaa acttatttaa atcattctga agtccatcaa aatttttcga
     1201 aagtaaaccc ggtgccaacg tcacttccag catatgttct tcaaaattaa cattgttccc
     1261 cacttttaaa cctcgtgtac tttcaaatac ttgaacaaag gcattggtat ctattacttt
     1321 tagcacttcg gccattaaca ttttaccgtc taattcaatg taacatattt cattttgagc
     1381 tactggtccg tcaatttcca ccgttactaa attagaaaca acacctttta cttttccttt
     1441 tgttgacata atttttttta atacagcttt taacaaaatt aattttttga cctttattct
     1501 atttagtcat ttctattgtt tttttacatt atctaaaata tcttcttg
//
LOCUS       DAOTKS010000385         1544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_65670_length_1544_cov_5.435191, whole genome shotgun sequence.
ACCESSION   DAOTKS010000385 DAOTKS010000000
VERSION     DAOTKS010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65670_length_1544_cov_5.435191"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..466)
                     /gene="rpsI"
                     /locus_tag="P5236_06735"
     CDS             complement(80..466)
                     /gene="rpsI"
                     /locus_tag="P5236_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRU64093.1"
                     /translation="MEVINALGRRKTAVARIFVSEGSGKITINKQDLSQYFPSPLLQY
                     IVKQPLNKLNVAEKYDIKVNLNGGGFKGQAEALRLAIARSLVKINPEDKKLLKSEGFL
                     TRDPRIVERKKPGRPKARKRFQFSKR"
     gene            complement(472..927)
                     /gene="rplM"
                     /locus_tag="P5236_06740"
     CDS             complement(472..927)
                     /gene="rplM"
                     /locus_tag="P5236_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRU64094.1"
                     /translation="MDTLSYKTISANKATVQKEWVLIDATDMVLGRMGSKVAKLLRGK
                     YKPNFTPHVDCGDNVIIINARKVKLTGNKWTDRVYLRYTGYPSGQRETTPAKIMEKDP
                     EKLIRRVVKGMLPKNRLGAQLLKNLYIFPDEEHNMQAQKPKQIDLNSLK"
     gene            1198..>1544
                     /locus_tag="P5236_06745"
     CDS             1198..>1544
                     /locus_tag="P5236_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HRU64095.1"
                     /translation="MSRLDFTFYAKDAVTVAENLLGKILIRRWEDGSFSRYIITETEA
                     YFGEEDKACHASKGRTPRTEIMYASGGHVYVYFIYGMYWMFNVVTGPENHPEAVLIRG
                     LNDIIGSGKVGREL"
BASE COUNT          477 a          258 c          242 g          567 t
ORIGIN      
        1 aactactttt taatttctta gcatgaaaac ttttctgtaa aaaaatatca tgcaaaaaga
       61 atagatataa aatttgaaat taacgttttg aaaattgaaa tctcttccgt gctttgggac
      121 gtccaggttt tttacgctct acaatacgtg gatcacgagt taaaaagcct tctgatttta
      181 aaagtttctt atcttcagga ttaattttta ctaatgaacg agcaattgcc aaacgtaatg
      241 cttctgcctg ccctttgaaa ccaccgccat tgagatttac cttgatatca tatttctcag
      301 ctacatttaa tttattaagc ggttgcttta caatatattg taaaagtgga gaaggaaagt
      361 actgtgaaag atcttgctta ttaatagtaa tcttaccgct tccttcgcta acaaaaatac
      421 gggcaactgc tgtttttcta cgacctaaag catttattac ttccatactt attatttaag
      481 tgagtttaaa tcaatttgtt ttggtttttg tgcctgcata ttgtgttctt cgtcagggaa
      541 aatatacaaa tttttcagca attgtgctcc taaacgattt ttgggcaaca ttcccttgac
      601 tactcttcgg attaattttt ctggatcttt ttccataatc tttgctggcg tagtttcacg
      661 ctgaccgctt ggatatccgg tatatcgtaa atacactcga tctgtccatt tatttccggt
      721 taattttacc tttctcgcat tgataataat cacattatca ccacaatcta catgaggcgt
      781 gaaattaggt ttatatttcc ctcttaaaag ttttgcaact ttagaaccca ttctgcctaa
      841 caccatatct gtagcatcaa ttaaaaccca ttccttttgt acagttgctt tattggctga
      901 aatggtttta taacttaagg tatccacttt ataaaatttt tttagtaatt taattcggtt
      961 tccttattta attaatttcc tttctttctt caactaaaaa tgaatcactt aaaataaaaa
     1021 atcaactttc tattctcatt cgaagaaaga aataaatgga ttgcaaaaat actgtttttc
     1081 tttggattga caaaacaatc attaaatatt ttctttaaaa aagagaaaga aaaatattaa
     1141 ttttgccttt tgtaggagtt ttttaatgaa tcttttattt tttcgattta tataattatg
     1201 tctcgattag attttacatt ctatgctaag gatgctgtta cagtagccga aaatttatta
     1261 ggtaaaattc taattcgtcg ctgggaagat ggttcttttt cacgatatat catcactgag
     1321 acggaagcct attttggtga agaagacaaa gcctgtcatg ccagcaaagg aagaacccca
     1381 cgaacagaaa ttatgtatgc ttcaggtgga catgtttatg tttactttat ttatggaatg
     1441 tactggatgt tcaatgtggt gacaggtcca gaaaatcatc cagaagcagt attgattcga
     1501 ggtttaaatg acataatcgg ttcagggaaa gtaggacgcg aact
//
LOCUS       DAOTKS010000386         1531 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_66475_length_1531_cov_2.825881, whole genome shotgun sequence.
ACCESSION   DAOTKS010000386 DAOTKS010000000
VERSION     DAOTKS010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66475_length_1531_cov_2.825881"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="P5236_06750"
     CDS             complement(<1..201)
                     /locus_tag="P5236_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRU64096.1"
                     /translation="MNKYLDKKISQIQARIKLPASKSLSNRALILNALAKSPYPIENL
                     SDSDDTVVMINALNSTAHSFNVG"
     gene            complement(363..1529)
                     /locus_tag="P5236_06755"
     CDS             complement(363..1529)
                     /locus_tag="P5236_06755"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRU64097.1"
                     /translation="MDNFNFYSPTYFVFGRGRENEAGKYVKRFSGSKVLLHYGGGSAI
                     KSGLLDRVKKSLKMENINFLELGGVKPNPRSGLVYQGIEICKKEKIDFLLAVGGGSVI
                     DSAKAIAAGALYDGDFWDFYCGKRVEKALPVGTILTIAAAGSEGSDSSVITNENGMLK
                     RSAEGDALRPVFSILNPELTFTLPAYQTACGITDMMAHVMERYFTNTKEVEITDRLCE
                     GILLAIINEATKVIAEPDNYDARANIMWAGMVAHNDICGVGREQDWATHGMEHELSAL
                     YDVAHGAGLAVMFPAWMKYVMHHDIMRFAQFAVRIWGCQMDFQHPENAAKKGIEKLEQ
                     FLVSIGMPIRFAQLGAKAEDIPLLVKNMQLGDKTLGSFVKLTENDIRKIYELAV"
BASE COUNT          469 a          331 c          245 g          486 t
ORIGIN      
        1 tcctacatta aaagaatggg ctgttgaatt taaagcattt atcataacca ccgtgtcatc
       61 cgaatcagaa agattttcaa tcggataagg acttttagca agagcgttca gaatcaaagc
      121 tcgattactt agacttttag aggcaggtag tttaattctt gcctgaattt gtgatatttt
      181 tttatctaag tacttgttca tcagaagaat atttaataac gtaggcttta tttttcatta
      241 aaaagagttt gttctcttat tgagtttttg tatagatatt acccggcgaa ccaaaaaata
      301 attgaatttg catttaaaaa attcagtcct aacataaaat aattttcttt tggagtgaac
      361 tgtcacacag ctaattcgta aatttttctt atatcatttt ctgttagttt cacaaaactg
      421 cccagtgttt tatcacccaa ttgcatgttt ttcaccagca aaggaatatc ttcagcttta
      481 gcacctaatt gtgcaaaacg gataggcatg ccaatggaaa ccagaaattg ttccagcttt
      541 tcaattccct ttttagctgc attttcagga tgctgaaaat ccatctggca accccagata
      601 cgaactgcaa attgagcaaa acgcataatg tcatgatgca tcacgtattt catccaggca
      661 gggaacatta ctgctaaacc agctccgtga gctacgtcat ataaagccga caattcatgt
      721 tccattccat gagttgccca atcctgttca cgacccacgc cgcaaatatc gttatgtgca
      781 accattccag cccacataat attagctcgg gcatcataat tatctggttc ggcaataact
      841 ttggtcgctt cgttgataat tgccagtaaa attccttcac aaaggcggtc ggtaatttct
      901 acttctttag tgttggtgaa atatctttcc atcacatgag ccatcatatc agtaataccg
      961 caagctgttt gataagctgg taaagtaaaa gtgagttctg gatttaaaat agaaaaaaca
     1021 ggacgcaaag catcgccttc ggctgaacgt ttcaacatac cattttcgtt ggtaataaca
     1081 ctgctgtctg aaccttcgct accagctgct gcaatagtaa ggattgttcc aactggaaga
     1141 gctttttcaa ctcttttccc gcaataaaag tcccagaaat cgccatcgta taaagcacct
     1201 gctgcaatag cttttgcaga atcgatcaca ctaccacctc caacagccag aagaaaatca
     1261 attttttctt ttttacaaat ttctattcct tgatatacca acccactgcg tggattaggc
     1321 ttcacaccac ccaattccag aaaattaata ttctccattt ttagtgattt tttgacccga
     1381 tccagtaaac cacttttaat agctgaacct cctccatagt gaagcagtac ctttgagcca
     1441 ctaaaacgtt ttacgtattt tcctgcttcg ttttcgcgtc ctcttccaaa cacaaaataa
     1501 gtaggactat aaaaattgaa attgtccata t
//
LOCUS       DAOTKS010000387         1530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_66542_length_1530_cov_2.324068, whole genome shotgun sequence.
ACCESSION   DAOTKS010000387 DAOTKS010000000
VERSION     DAOTKS010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66542_length_1530_cov_2.324068"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="P5236_06760"
     CDS             <1..608
                     /locus_tag="P5236_06760"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HRU64098.1"
                     /translation="KKRPFVTLKWAQTIDGFIDQNRTDVLQLPLNISNHFTKILTHKI
                     RSENQAILVGTNTVILDNPSLTVRYWWGKSPIRIAIDRLGKVPENYHLLDGNTPTIIF
                     TEQLRQNKQNLKYIPINFDKDILSTVLQKIYELNIHSILVEGGAQLLTNFIISNLWDE
                     AYVEVAPFEIGKGVAAPIMPLMPYARKNFYGHEWYHYKNKK"
     gene            616..>1530
                     /locus_tag="P5236_06765"
     CDS             616..>1530
                     /locus_tag="P5236_06765"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRU64099.1"
                     /translation="MSKFITIFFIALYVYTVLSTISVLLLENRNPAKSFSWMLIILFL
                     PVIGLLLYMTFGQDLRKRKIISRKSLHNLPERVIAPFDITEVEQLLNNLEKCQKNLIK
                     LLYKNSEAIAYPANKVQVYSDGQNAFDAIFDAIRAAQNHIHIEFYILSNDKVAQQLKE
                     LLIQKSQEGVRVRMIYDYIGSLNLSRKYIKSLTKAGVYVRSFLPLRLRIFRSRINYRN
                     HRKIMIIDGKIGFTGGMNIADRYIHGNRLGEWRDNFVRIEGAAVHGLQHLFLADWYFV
                     ERKLITAPKYYPQPENFNTNNLIQIVSSG"
BASE COUNT          529 a          260 c          248 g          493 t
ORIGIN      
        1 aaaagaaacg tccatttgtt acactaaaat gggctcaaac tattgatgga tttattgatc
       61 aaaacagaac ggatgttcta caattaccgc tgaacatttc caatcatttt acaaaaattc
      121 ttacacataa aatacgttct gaaaatcaag ccattctcgt tggcaccaat acagttattt
      181 tagataatcc ttccttaacc gttcgatact ggtggggaaa atcacctatc agaattgcga
      241 tagatcgtct tggaaaagtt ccagaaaatt atcatttact tgatgggaat acccctacca
      301 ttatttttac ggaacaacta cgccaaaata aacaaaattt aaaatacatt ccaataaatt
      361 ttgataaaga tattttatca actgttttac aaaagattta tgaactgaat attcattcca
      421 ttttagtgga aggcggtgct caattattaa caaattttat tatttccaac ttgtgggacg
      481 aagcttatgt agaagtagca ccttttgaaa ttggtaaagg cgtagccgca cccattatgc
      541 cactaatgcc ttatgcaagg aaaaattttt atgggcatga atggtatcat tataagaata
      601 aaaaataaat aagaaatgtc aaaatttatt actatttttt ttattgcttt gtatgtatat
      661 actgttctta gcactatttc agttttactg ctcgagaaca gaaatccagc aaaatctttt
      721 tcatggatgc tgatcatctt gttccttcct gtaattggct tactgcttta tatgactttt
      781 ggacaagatc tacgcaaacg aaaaatcatt tccagaaaaa gtcttcataa cttacctgaa
      841 cgagtcattg ctccttttga tattacagaa gtagaacaat tattgaataa ccttgaaaaa
      901 tgtcagaaaa atctcatcaa acttttgtat aaaaacagcg aagccattgc ttatccagcc
      961 aataaagttc aagtttattc ggatggtcaa aatgctttcg atgccatttt tgacgccatt
     1021 cgtgcagctc aaaatcatat ccatattgaa ttttatattt taagtaatga taaagttgcc
     1081 caacagttaa aagaattgtt aatccaaaaa tcacaggaag gagttcgggt ccgcatgatt
     1141 tatgattata tcggcagctt gaatttatcc agaaaatata taaaatcttt aacaaaagcc
     1201 ggtgtttatg tccgctcctt cttacctttg cgattaagaa tatttagaag cagaatcaat
     1261 tatcgaaatc acagaaaaat tatgattatt gacgggaaaa ttggtttcac gggcggaatg
     1321 aatatagctg atcgatacat acatgggaac cgattagggg aatggcgtga taattttgtt
     1381 cggattgaag gagcagcagt gcatggattg caacatttat ttcttgccga ctggtatttt
     1441 gtagaacgaa aactgattac agctccaaaa tattatcctc aacctgaaaa ttttaacacc
     1501 aataacttga ttcaaattgt ttccagcgga
//
LOCUS       DAOTKS010000388         1528 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_66651_length_1528_cov_4.915139, whole genome shotgun sequence.
ACCESSION   DAOTKS010000388 DAOTKS010000000
VERSION     DAOTKS010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66651_length_1528_cov_4.915139"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1517)
                     /locus_tag="P5236_06770"
     CDS             complement(<1..1517)
                     /locus_tag="P5236_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRU64100.1"
                     /translation="MSVIEKLKEAKKRFQQYGPNQLETIKQKTTWQIFISQFRGSMVM
                     ILFVAAIISGVIGLRNGEGLIDTIVILAILIVNAIIGTVEEKKAQSSLEALKKMSAPH
                     SKVLRDGEIIEIPTIEIVPGDIVVLDTGDIIPADMRLIEAVNLKIQESSLTGESVPVE
                     KNTETLNTENVPLGDRKNMAFSTCVVTFGRGKGVVVATGMKTEVGNIAHMLQHTEETE
                     TPMGKRLEQLGKILGYVAIIICAVIFIVGLLYGNDWLDMFMMAVSLAVAAIPEGLQIV
                     STLVLSIGVQRMVKLNAIVRRLPSVESLGSVTVICSDKTGTLTQNKMTVVKSWTIENE
                     IDFRNQNISEKWNNTQQILLQSALLCTDAHLRILPDGTHENAGDPTETAIVDIALNLG
                     INKNEEEKKFPRIAEVPFDSERKRMATINKMENGKLRVNVKGGIDEVLSVTSYLLVQD
                     KVRPITDEDITIIRKQNENMAGKALRILAVAYKDIDKLPDKVTVETIENNLIFVG"
BASE COUNT          458 a          322 c          241 g          507 t
ORIGIN      
        1 aatcctacaa aaataagatt gttctcaatg gtttcaacgg tcaccttgtc gggaagttta
       61 tcaatatcct tataggctac tgcaagaata cgtaaagcct ttcctgccat attctcattc
      121 tgttttctga taatagtaat atcttcatca gtaattggac gaactttatc ttgcaccaaa
      181 agataagaag tgacacttaa tacttcatct attcctcctt tcacgttaac acgtagtttc
      241 ccgttttcca ttttgttgat agtagccata cgcttacgtt ccgaatcgaa tggaacttca
      301 gcgatgcgtg gaaatttttt ttcttcttca tttttattta ttcccagatt caaggctata
      361 tccacaattg ctgtttcagt tggatcacct gcattttcgt gagtgccatc gggaagaatt
      421 cttagatgag catcagtaca aagcaatgcc gattgtaaca gaatttgttg ggtgttgttc
      481 catttttcag aaatattttg attacgaaaa tcaatttcat tttctatagt ccaacttttt
      541 accactgtca ttttattctg agtaagagtg ccagttttat cggaacaaat cacggtaacg
      601 ctacccaatg actctaccga aggcagtcta cgtacaatag cattaagttt taccattcgt
      661 tgtaccccaa tagaaagcac caaagtagaa acaatctgaa gcccttcggg aatggcagca
      721 actgcaaggc taacagccat cataaacata tccagccaat cgttcccgta aagtaaacct
      781 acaataaaaa taacagcaca aataataatt gcaacataac ctaaaatttt gccaagctgt
      841 tctaaccttt tccccatcgg cgtttcagtt tcttcggtat gttgaagcat atgggcaata
      901 tttcccactt cagttttcat gccagtagct actacaacac ctttgcctcg tccgaaagtt
      961 actacacagg tagaaaatgc catatttttt cgatcaccta aaggaacatt ttctgtattc
     1021 aaagtttcgg tatttttctc tacgggtacc gattctcctg tcaaggaaga ttcctgaatt
     1081 ttaagattga ctgcttctat cagtcgcata tcagcaggaa taatatcacc tgtatccagc
     1141 actacaatat ccccaggaac tatttcaatg gtagggattt caataatttc tccatcacgt
     1201 aaaaccttag aatgtggagc actcattttt ttaagagcct ctaaagagga ttgagctttt
     1261 ttctcttcta ctgttccaat aatggcatta acaataagga tagcaagtat aacaatggta
     1321 tcaattaatc cctctccatt tcgtaaccca atcacacctg aaataatagc agccacaaaa
     1381 agaatcatca ccattgatcc tctgaattga ctgataaata tttgccaggt agttttttgt
     1441 tttatggttt ctaattgatt gggaccatat tgctgaaaac gtttttttgc ttcttttagt
     1501 ttttcaatta cagaaattga gtgtccat
//
LOCUS       DAOTKS010000389         1527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_66723_length_1527_cov_3.690217, whole genome shotgun sequence.
ACCESSION   DAOTKS010000389 DAOTKS010000000
VERSION     DAOTKS010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66723_length_1527_cov_3.690217"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..1203
                     /gene="thiL"
                     /locus_tag="P5236_06775"
     CDS             166..1203
                     /gene="thiL"
                     /locus_tag="P5236_06775"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HRU64101.1"
                     /translation="MPRTEISTLGEFGLIHHLTDNCKINNLSTIKGVGDDAAVLDYKN
                     KYVLVATDLLLEGVHFDLVYVPLKHLGYKAAVVNFSDIYAMNGEPKQITISLGISRKF
                     SVEDLDQIYEGIKLACDNYGVDIVGGDTSTSLTGLTISITCIGEGEKDKIVYRNGAKV
                     NDLICVSGNLGASYLGLQLLEREKMVLEENQQSQPRFEGYEYLLERQLKPEARGDIIR
                     MLREKEVLPTAMMDISDGLSSELMHICTQSNVGCRIYEDKIPIHYQTAKMAEEMNLNV
                     VTAALNGGEDYELVFTASLNDYDKIIMMEDVNIIGYVTKPEMGYYLIGREGEEIQLQA
                     QGWNSLKQGNM"
BASE COUNT          556 a          198 c          273 g          500 t
ORIGIN      
        1 attgataatg aataaattga gaatatattc tcaatctttt gacaagaaaa ggaaattgaa
       61 tatctttgtt tcatcaaata atataaataa aatttcctaa attttttaat gcactatttc
      121 tttctctctt catcgtaaat tacaaatttt ttatttccat catatatgcc acgaactgaa
      181 atttctacat taggtgaatt tggcttgatt catcatttaa cggacaactg taaaattaat
      241 aacctttcca ccataaaagg tgtaggtgat gatgcagcgg tgcttgatta caagaataaa
      301 tacgttttag ttgccaccga tcttttattg gaaggtgttc attttgatct ggtgtatgtt
      361 cctttaaaac atttaggata caaagctgct gtagtcaatt tttcggatat ttatgcaatg
      421 aatggtgaac caaaacaaat taccatttca ttaggtattt ccagaaaatt ttcagttgaa
      481 gatttggacc agatttatga aggaataaaa ttggcttgcg acaattacgg cgttgatata
      541 gtaggaggcg atacttctac atcattgaca ggacttacca tcagcattac ctgtattggt
      601 gaaggtgaaa aagataaaat tgtataccgc aatggtgcta aagtaaatga tttgatttgt
      661 gtctcaggca atttgggtgc atcgtacttg ggtttgcaat tgttggaacg agaaaaaatg
      721 gttcttgaag aaaatcagca atcccaacca cgttttgaag gttacgaata cctgttagaa
      781 cggcaattaa aacctgaagc ccgtggagat attattagga tgttacgaga aaaagaagtt
      841 ttaccaacag ctatgatgga tatttccgat ggcttgtctt ctgaattgat gcatatttgc
      901 acacaaagta atgttggctg cagaatttat gaagacaaaa ttcctattca ttatcaaacg
      961 gcaaaaatgg ctgaagaaat gaacctgaat gtagtaactg cagctttaaa tggtggtgaa
     1021 gattatgagt tggttttcac agcttcactg aatgattatg ataaaattat catgatggaa
     1081 gatgttaaca tcattggcta tgttacaaaa ccagaaatgg gatattattt aattggtcgt
     1141 gaaggcgaag aaattcagct tcaagcccag ggttggaatt cactaaaaca agggaatatg
     1201 tgatattttt aacaagaaaa gtttattgga gaaattgata tttggacggt ttaattaaaa
     1261 aaatattact ataacgtttt caagtgcgta aaaaatggtc tgaaaaggaa gagctaataa
     1321 gttagagtag agaatttttc aaaaaaataa aaaattaagt ttaatacaac aattaaagga
     1381 aaggaaaaga aacaaattcg catattttaa tttattttaa tatacttttt tgcataacta
     1441 tacgtttttt tttttagaat taaattaaaa aactattttg cttatgcaaa aaaaatctac
     1501 ttcaaaaaaa aaaactaaat taagcaa
//
LOCUS       DAOTKS010000390         1525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_66831_length_1525_cov_5.642857, whole genome shotgun sequence.
ACCESSION   DAOTKS010000390 DAOTKS010000000
VERSION     DAOTKS010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66831_length_1525_cov_5.642857"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="P5236_06780"
     CDS             complement(<1..1317)
                     /locus_tag="P5236_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HRU64102.1"
                     /translation="MDNKFQQNKPQNPGPKTPKFNISWIYAIIIAILIGSYLFTDTSP
                     VKEVPYSDFKSYMNTRKIEKIAIYSSRNVAEAQIVKDSLKEVFGDKYEMYAKERKVRT
                     RIPSVEVFSSDYQKAQDDYGLNAVVEYKESKNYIDIFLYSILPFLLLILFFVFMNRRM
                     AKQMGGSNSGGGIFSVGKSKAQLFDKNTSNRITFKDVAGLAEAKQEVEEIVSFLKNPS
                     KYTELGGKIPKGVLLVGPPGTGKTLLAKAVAGEADVPFFSISGSEFVEMFVGVGASRV
                     RDLFRQAKEKAPCIIFIDEIDAIGRARGRNPNFGGNDERENTLNQLLTEMDGFASNSG
                     VIILAATNRADVLDKALLRPGRFDRQIHVDLPDVNDRKEIFKVHLRPIKINKTVDVDF
                     LAKQTPGFSGADIANVCNEAALIAARKNKTFVDKQDFLDAVDRIIGG"
BASE COUNT          469 a          300 c          249 g          507 t
ORIGIN      
        1 tccaccaatg attcggtcta ctgcatcaag gaaatcttgt ttgtcaacaa aagttttatt
       61 ttttcgcgct gcaatcaaag ccgcttcgtt acaaacattt gcaatatcag cacctgaaaa
      121 gccaggtgtt tgttttgcta aaaaatcaac atcaactgtt ttattaattt taatagggcg
      181 taaatggact ttaaaaattt ctttacggtc atttacatct ggtaaatcaa cgtgaatctg
      241 tctatcaaaa cgacctggtc ttagtaaagc tttgtctaaa acatcggcac ggttggtggc
      301 tgccaaaata ataactccgc tattggaagc aaagccatcc atttcggtaa gtaattgatt
      361 cagtgtgttt tctctttcat cattaccacc aaaatttgga tttctaccac gagctctccc
      421 aatagcatca atttcatcaa taaaaataat gcatggtgct ttttcttttg cttgtctaaa
      481 taaatcgcga acgcgtgaag caccaactcc tacaaacatt tccacaaatt cagaacctga
      541 aatagaaaag aaaggaacat ctgcttcgcc tgctacagct tttgccagta atgtttttcc
      601 tgttcctgga ggacctacca ataaaacacc ctttgggatt ttacctccta attcggtata
      661 cttacttgga tttttcaaaa atgatacgat ttcttccact tcctgtttgg cttcagcaag
      721 tcctgccaca tctttaaaag tgatacggtt gctggtgttt ttatcaaaaa gctgtgcttt
      781 cgatttacct acactaaaaa ttcctcctcc gctgttactt cctcccattt gctttgccat
      841 acggcgattc ataaacacga agaaaagaat caataataaa aatggaagta tgctatacaa
      901 gaaaatatca atataatttt tactttcctt gtattctacc acagcattta gaccatagtc
      961 atcttgggct ttctgataat ccgaagaaaa aacttccact gatggaatgc gagtccttac
     1021 ttttcgttct tttgcataca tttcatattt atcaccaaag acttctttaa gagaatcttt
     1081 tacaatttga gcttcagcaa catttcgcga agaataaatg gctatttttt ctatttttcg
     1141 cgtattcata taagatttaa aatctgaata aggaacttct ttcaccggcg aagtatcagt
     1201 aaataagtag gaaccaatca gtatagctat aatgatagca taaatccacg atatattaaa
     1261 ttttggagtt tttggtccag ggttttgagg tttattttgt tgaaatttat tgtccatttt
     1321 taaatcaaca ttaacttata tttctaaaaa aaatcaaatt attattgttt tattacaaaa
     1381 cattttcaat ttcaattacc ggctgatcga ggggtggcaa agtgggtgtg gtcgcagaca
     1441 gcgataaact gaccggtggt tgaacggttg aaacagcgac atttttgggt tgggcactgt
     1501 cccaaagcca aagcaccagt agcgt
//
LOCUS       DAOTKS010000391         1494 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_68958_length_1494_cov_3.861015, whole genome shotgun sequence.
ACCESSION   DAOTKS010000391 DAOTKS010000000
VERSION     DAOTKS010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68958_length_1494_cov_3.861015"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..943)
                     /locus_tag="P5236_06785"
     CDS             complement(77..943)
                     /locus_tag="P5236_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004290876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster domain-containing protein"
                     /protein_id="HRU64103.1"
                     /translation="MNVILISVLSLGGIGALSALILYFVAQKFQIFEDPRIDEVEAVL
                     PAANCGGCGYPGCRAFATACVKAESLDNLVCPVGGAPVMEKVASILGKTAATITSTIA
                     VVRCGGSCEYRPRVNVYDGVKTCAIVHQLYQGETLCSWGCLGFGDCEAVCTFDAIHIN
                     PQTLLPEVDEEKCTSCGLCVKACPRNIIELRQKGPKSRRIYVNCVNQDKGAVNKKVCT
                     VACIGCSKCQQVCNYDAIIIANNLAYIDDEKCKLCRKCVDVCPTNSIVEINFPPRKTK
                     TETSSNISITEQ"
     gene            complement(994..1416)
                     /locus_tag="P5236_06790"
     CDS             complement(994..1416)
                     /locus_tag="P5236_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008660517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HRU64104.1"
                     /translation="MSQSIEHSGIVEQIDGDQIKVLIMQQSACSECHAKSLCSIADKK
                     EKIIEVISSDPTLKVGDKVIIYGESSIGLQAVLLAFIIPFVLIFLALFIFQHFIENEL
                     IAGILAILVLVPYYLILFLSNKRLKNKFQFKIKKEDTI"
BASE COUNT          504 a          269 c          218 g          503 t
ORIGIN      
        1 agataaattc ctcctatttt gaaagttttc attggatagt taagtaaatt ttatttttct
       61 tgtaaaaatt gaaaaattat tgttctgtaa tagaaatatt ggaagatgtt tctgtttttg
      121 ttttgcgtgg aggaaaattt atttccacta ttgaattagt agggcaaaca tccacgcatt
      181 ttctacacaa tttacatttt tcgtcatcaa tataagcaag gttgtttgct atgataatag
      241 catcataatt acaaacttgt tgacattttg aacaaccaat gcaagcaacc gtacatactt
      301 ttttattaac agctccttta tcttgattaa cacaatttac gtaaattctt cttgatttgg
      361 gacctttctg acgaagctca ataatatttc gcggacaagc ttttacgcaa agtccacatg
      421 aagtacattt ttcttcatca acttcaggta acagggtctg tggattgata tgaatggcat
      481 caaaagtaca aacagcttca caatcgccaa aacccagaca accccaacta caaagtgttt
      541 ctccctgata aagttgatga acaatggcgc aagtttttac tccgtcataa acattgacac
      601 gaggacgata ttcacaactc ccaccacatc gcactacagc aatcgttgaa gtgatggttg
      661 ctgctgtttt tcctaaaata gaggctactt tttccattac gggagctcca ccaactggac
      721 aaaccaaatt atctaacgac tcagctttaa cacaagcagt tgcaaaagct cgacatcctg
      781 gataaccaca gcctccgcaa ttagcggcag gtaaaactgc ttcaacttcg tcaattcgcg
      841 gatcttcgaa aatctgaaat ttttgtgcca caaaatataa aatcagagct gataatgctc
      901 cgattcctcc taaagaaagt actgaaatta atattacgtt cattttgatt tatctcttga
      961 gtttaaagtt gttcaaaaaa attttttact tccttaaatt gtatcttctt ttttaatttt
     1021 aaattgaaac ttgtttttta atcgtttatt cgataaaaaa agaataagat aataaggaac
     1081 gagcaccaga atggctaaaa ttcctgctat taattcattt tcaatgaaat gttgaaatat
     1141 aaacaaagct aaaaaaatta atacgaaagg tatgatgaaa gccaataata ctgcttgaag
     1201 tcctattgaa ctttctccat aaataataac tttgtcaccc acttttaaag taggatcaga
     1261 actgattact tcaataattt tttctttttt atcagcaata gagcataaac ttttagcatg
     1321 acattcgctg catgccgatt gctgcataat cagtactttt atttgatcgc catcaatttg
     1381 ttcaacaata cctgaatgtt ctattgattg actcattttg taatgtagac attgcaattt
     1441 ttgagcacaa aggtactgat tatttattaa tgactaaaaa aatattatag aaaa
//
LOCUS       DAOTKS010000392         1464 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_70964_length_1464_cov_4.395316, whole genome shotgun sequence.
ACCESSION   DAOTKS010000392 DAOTKS010000000
VERSION     DAOTKS010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70964_length_1464_cov_4.395316"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="P5236_06795"
     CDS             <1..525
                     /locus_tag="P5236_06795"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRU64105.1"
                     /translation="SVKKSELNVVKRFLVANFGEEAALDALQILKKLLEKEIDIAPVT
                     AQINQYMNYPIKLEILHLLLEIAYADAEVSSSEMNAIQRIAYLLKISSSDFESLKAIY
                     VKHEDHQWAYKALEIESTASNEEIKKAYREMAKKYHPDKVNTLGEDIKKIATEKFRSI
                     NEAYEVLKKERGFA"
     gene            564..>1464
                     /gene="cysS"
                     /locus_tag="P5236_06800"
     CDS             564..>1464
                     /gene="cysS"
                     /locus_tag="P5236_06800"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRU64106.1"
                     /translation="MENQLYIYNTLHRQKELFVPLQPPHVGMYVCGPTVYGDPHLGHA
                     RPAITFDILFRYLKHLGYKVRYVRNITDVGHLENDVDSGEDKIAKKARLEQLEPMEVA
                     QYYMNRYHKAMEALNVLPPSIEPLASGHIMDQIDFVEKILKNGFAYVSEGSVYFDVEK
                     YDKIYHYGKLSGRNIADLLEATRSLEGQEEKRKSYDFALWKKTSPEHIMRWKSPWSEG
                     FPGWHMECSAMSTKYLGKHFDIHGGGMDLIFPHHECEIAQSTAALGSESVKYWMHNNM
                     VTINGQKMGKSLGNFITLEEFFTG"
BASE COUNT          518 a          222 c          296 g          428 t
ORIGIN      
        1 agtgttaaaa aatcagaatt aaatgtggtg aaacgttttc tggtagcaaa ttttggtgaa
       61 gaagctgcat tggatgcctt acaaatattg aaaaaattat tagaaaagga aattgatatt
      121 gctccagtca ctgcacaaat aaatcagtat atgaattatc ccattaaatt agaaatatta
      181 catttgttgc tggaaattgc ttatgctgat gctgaggttt cttcttcaga gatgaatgcg
      241 attcaaagga ttgcctatct tttaaaaatt tcttcttctg attttgaatc actaaaagca
      301 atttatgtca aacatgaaga tcatcagtgg gcttataaag cacttgaaat cgaatcaacc
      361 gcttctaatg aagagattaa aaaagcatac agagaaatgg caaaaaaata tcatcccgac
      421 aaagttaata cgctgggaga agacattaaa aaaatagcaa ctgaaaagtt tcgttccatc
      481 aatgaagctt atgaagtttt aaagaaggaa agaggttttg cttgaaaaaa tttataataa
      541 ttagaagaaa caaatgataa cttatggaaa atcaattata catttataac actttacatc
      601 gacagaagga gttgttcgtt ccacttcaac cgccacatgt aggaatgtat gtttgcggac
      661 caactgttta cggtgatccc catctggggc atgcacggcc tgccattact tttgatatcc
      721 tgtttcggta tttaaaacat ttaggataca aagttcgtta tgttcgtaat attacagatg
      781 taggtcatct ggaaaacgat gtcgacagcg gtgaagacaa aattgctaaa aaagcacgac
      841 tggagcaatt ggagccaatg gaagttgctc aatattacat gaatcgctac cataaagcaa
      901 tggaagcatt aaatgtatta ccccccagca ttgaacccct tgcttcagga catatcatgg
      961 atcagattga ttttgttgaa aaaattctaa aaaatggttt tgcttacgta agtgaaggtt
     1021 cggtatattt tgatgtggaa aaatatgaca aaatttatca ttatggcaaa ctttcagggc
     1081 gaaacatagc tgatttgttg gaagcaactc gttcgctgga aggacaagaa gaaaaaagaa
     1141 aaagttatga ttttgctttg tggaaaaaaa cttcaccaga acatatcatg cgatggaaat
     1201 cgccgtggag cgaaggtttt ccgggttggc acatggaatg ttcagccatg agtacgaaat
     1261 atttaggcaa acattttgac atccatggtg gaggaatgga tttgatattt ccccatcacg
     1321 aatgtgaaat tgctcaatct acggctgctt taggaagtga aagtgtaaaa tattggatgc
     1381 acaataacat ggtgactatc aatggtcaga aaatgggaaa atcgttggga aattttatta
     1441 cactcgaaga attttttact ggca
//
LOCUS       DAOTKS010000393         1462 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_71101_length_1462_cov_5.751244, whole genome shotgun sequence.
ACCESSION   DAOTKS010000393 DAOTKS010000000
VERSION     DAOTKS010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71101_length_1462_cov_5.751244"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..898
                     /locus_tag="P5236_06805"
     CDS             101..898
                     /locus_tag="P5236_06805"
                     /inference="COORDINATES: protein motif:HMM:NF016013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HRU64107.1"
                     /translation="MENKEKKNLQKYWQKIYQKIRNITNLHEDTNIETTLESIKKDVE
                     FKGANVWILFFAVIVCSVGLNVNSTAVIIGAMLISPLMGPINGVGLAVGINDAELLRN
                     ALKNLLIMILVGLLASTLYFLITPLSDAQSELLARTRPTIFDVLIAFFGGLAGIVAAS
                     RKEGKITVIAGVAIATALMPPLCTAGFGLATGQMKYFGGAFYLLFLNSFFIALANLLM
                     VRYLHFPVKHFVDSTKESSPTFYYYIFYFSDYTKRFSCMECSQRKSI"
     gene            927..>1462
                     /locus_tag="P5236_06810"
     CDS             927..>1462
                     /locus_tag="P5236_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU64108.1"
                     /translation="MQKLDLFTETEIINVNREYSSKGGKIYLSLIGKPLNERQIQFLQ
                     NQLVEYGLKGVQLSIKQTTGTLDIGAQTAFISNLLDKKDAELHKKDSLIQALNEELLK
                     IKNLGIDEIQIAREVKVQFPEIEKISFSHSIFTDVHTLAQDTIATVYLIWKENPKNNE
                     LIKKLEEWLKVRLNWEKM"
BASE COUNT          498 a          192 c          261 g          511 t
ORIGIN      
        1 ttttaaaaca aaattgttct ttaatttttt tggaagcaaa ttgtgaagtg tcaatttgtt
       61 tagaaatgta acttggttta tatgcttaaa tcttaaagtt atggaaaata aagaaaagaa
      121 aaatttacag aaatattggc aaaaaattta tcagaagata agaaatatta ccaatcttca
      181 tgaagatacc aacatcgaaa ccactcttga atctataaaa aaagatgtgg agttcaaagg
      241 tgcaaatgtt tggattttgt tttttgctgt gattgtttgt tcagtaggat taaatgtaaa
      301 ttctacggca gtaattattg gtgctatgtt aatttctcct ttgatgggac ctattaatgg
      361 agttggatta gcagttggta ttaatgatgc agaattatta cgaaatgcac taaaaaattt
      421 attgattatg attttagtag ggcttttagc ttctactctt tattttctta ttactccatt
      481 aagtgacgct caatccgaat tgttggcaag aacacgtcca acgatttttg atgtactgat
      541 agcttttttt ggtggtttgg ctggaattgt agcagcttca cgcaaagaag gtaaaatcac
      601 tgtaatagct ggagttgcca ttgctactgc actgatgcca cctttgtgta cagcaggttt
      661 tggattagca acaggacaaa tgaagtattt cgggggagct ttttatttgc ttttcctgaa
      721 ttcttttttt attgctttag ctaacctttt aatggtgcgt tacttgcatt ttccagtgaa
      781 acattttgtg gattcaacaa aagaaagtag tccgacgttc tattactata ttttctattt
      841 tagtgattat accaagcgtt tttcttgcat ggaatgtagt caaagaaagt cgatttaata
      901 ccaatgctat tcgatatgtt caagctatgc aaaagcttga tttatttact gaaactgaaa
      961 ttattaatgt aaatagagaa tattcttcaa agggtgggaa aatttatctt tctttgattg
     1021 gtaaaccgtt gaatgaaagg caaatacaat ttcttcaaaa tcaattagta gaatatgggt
     1081 taaaaggagt tcaattgagt atcaaacaaa caactggtac tttggatatc ggagctcaaa
     1141 ccgcttttat ttcaaattta ttagataaaa aagatgctga acttcataaa aaagattctt
     1201 tgattcaggc tctcaatgaa gaattgttaa aaataaaaaa tttaggaatt gatgaaatac
     1261 aaatagcacg tgaagtaaaa gtgcaatttc ctgaaataga gaaaatttct ttttctcatt
     1321 caatttttac tgatgtgcac acccttgctc aagatactat cgccactgtt tatcttatat
     1381 ggaaagagaa tcctaaaaac aatgaactaa tcaaaaaatt agaagaatgg ttaaaagttc
     1441 ggctcaattg ggaaaaaatg aa
//
LOCUS       DAOTKS010000394         1447 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_72201_length_1447_cov_5.512931, whole genome shotgun sequence.
ACCESSION   DAOTKS010000394 DAOTKS010000000
VERSION     DAOTKS010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72201_length_1447_cov_5.512931"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..1332
                     /locus_tag="P5236_06815"
     CDS             88..1332
                     /locus_tag="P5236_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HRU64109.1"
                     /translation="MKTPKVEIITIGDEILIGQIVDTNSAWMAQELNKIGFEIFQITS
                     VHDDEKQIIDALDAAIKHVDIVLMTGGLGPTNDDITKKTLCKYFNTKLIFDSSILENI
                     KKVFASRPEFVINDLTRSQAFVPENCEVIQNTVGTAPIMWFEKDKKIIVSMPGVPYEM
                     KEAMSREVMPKLQAYFKTPKIIHKTVQVFGYSESDLAIKIADWEKNLPENIHLAYLPN
                     YGIVKLRLSGTSDNLLSLEFSLNQQIDALTLILGNAIVAYEDLPMEQVVAKLLLSQEK
                     TVVTAESCTGGNIAHRFTSHSGSSNYFKGSVVAYDNEVKINLLGVSTDDLEKFGAVSQ
                     PVVEQMAAGVRRLLNADIAVATSGIAGPTGGSPEKPVGTVWIAACSEEKLVSKLFHFS
                     NTREQNIERATQAALVMIKELL"
BASE COUNT          501 a          230 c          277 g          439 t
ORIGIN      
        1 tcagttagaa aaaattgctc aggaagaaga atcaaaaaaa atataaaata tcaaatttat
       61 tcatacaaca aaaattatag tttaagcatg aaaaccccta aagttgaaat tattacaatt
      121 ggagatgaaa ttttaattgg tcaaattgtt gataccaatt cagcctggat ggctcaagaa
      181 ttaaataaaa taggctttga aattttccaa ataacctctg tgcatgatga tgaaaaacaa
      241 attatagatg ctttggatgc cgctatcaaa catgtagata ttgtactgat gactggtgga
      301 ttaggaccga ctaatgatga catcacaaaa aagactttgt gtaaatattt taatacgaaa
      361 ttaatatttg attcttccat tttggaaaat attaaaaagg tgtttgcttc tcgaccagaa
      421 tttgttatta atgatttgac tcgttcacaa gcttttgtcc cagaaaattg cgaagtaatt
      481 caaaacaccg taggtactgc tcctattatg tggttcgaaa aagataaaaa aataattgtt
      541 tccatgccag gtgttcctta cgaaatgaaa gaagccatga gcagagaagt gatgcctaag
      601 ttgcaagctt attttaaaac gcctaaaatt atacacaaga ctgtccaggt ttttggatat
      661 tctgaatcgg atttagccat taaaattgca gactgggaaa aaaatttgcc cgaaaacatt
      721 catttagcct atttacctaa ttatggaatt gtaaagttgc ggttgagtgg cacttcagat
      781 aatcttcttt ctctggaatt ttcacttaat cagcaaattg atgctttaac actgatattg
      841 ggaaatgcca ttgttgctta tgaggacctt cccatggaac aggttgttgc aaaattattg
      901 ctttcgcaag aaaaaacggt agtaacggct gaaagttgta ccggaggaaa cattgctcat
      961 cggtttactt ctcattcagg aagttccaat tattttaaag gctccgttgt agcttacgat
     1021 aatgaagtaa aaatcaacct tttgggagtt tcaactgacg atcttgaaaa atttggagct
     1081 gttagccagc cagtggttga acaaatggct gctggcgttc gtcgcttatt aaatgctgat
     1141 attgctgtag ctacttctgg aatagcagga cctacaggtg gaagtccaga aaaacctgtt
     1201 ggcacagttt ggatagctgc ttgttcagaa gaaaaattag tcagtaaact ttttcatttt
     1261 agcaatacgc gagaacaaaa tatcgaaaga gctactcaag cagcgttagt aatgataaag
     1321 gaactattat aagataattt caaagaaaag aaaagaaaag actgttaaat tgttgtttaa
     1381 cagtctttta taattttgtc gggatgactg gattcgaacc agcgaccaca cgccccccag
     1441 acgcgta
//
LOCUS       DAOTKS010000395         1443 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_72530_length_1443_cov_4.481988, whole genome shotgun sequence.
ACCESSION   DAOTKS010000395 DAOTKS010000000
VERSION     DAOTKS010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72530_length_1443_cov_4.481988"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1443
                     /gene="mutL"
                     /locus_tag="P5236_06820"
     CDS             <1..>1443
                     /gene="mutL"
                     /locus_tag="P5236_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRU64110.1"
                     /translation="GEALASIAAVSQVELKTRRAEDELGTLIRIAGSRVLEQEPVQCD
                     IGTTVAVKNLFFNVPARRRFLKSDNVEKNHLLYEFYNIALVHPQVSFLFYDEEELIYN
                     LSPSNLKLRIENIFGKNLRKRWEQQLLPIESTTNLVKIYGYVGKPEYAQKSANQYFFV
                     NGRYMRHPYFHKAVLTAYEQMIHENESPNYFIYFEIDPKNIDVNIHPSKTEIKFENEQ
                     AIWSILLATIKEALGKFNIIPSIDFNSEGDVKLPPKSSMGNVVPPQPSFNPYFNPFGD
                     TTYQRTPLNWEELYKGFEKNKGMTIEKQSPDETLFDSMKYSSDEKGKIFDNNLLATSY
                     FQLKNHYVLTGIKSGLLIIDQHKAHLRILFETFLNQMELQKGFSQQLLFPETLKLTVN
                     DALFFETIIEPLKKTGFIIENKGNNMYEIKGIPASLESVNIIELLRDLIEKSKTTEED
                     PSLHIKETIALTLSKAASLKVGQALTAEEMS"
BASE COUNT          503 a          234 c          246 g          460 t
ORIGIN      
        1 tggagaagct ttggcttcca ttgctgcagt ttcacaagta gaactgaaaa cgcgtcgtgc
       61 tgaagacgaa ttaggaacac taatcaggat cgcaggttca agagttttag aacaagaacc
      121 tgtacaatgt gatattggca cgacggtagc agtaaaaaat ctttttttta atgttcctgc
      181 acgtcgacgt ttcttaaaaa gtgataatgt agaaaaaaat catttgctat acgaattcta
      241 taatattgca ctggttcatc cccaagtttc atttttgttt tatgatgaag aagaattaat
      301 ttataatctt tctccttcga atttaaaatt aagaattgaa aatatttttg gaaaaaactt
      361 aaggaaacgc tgggaacaac aattgcttcc aattgaatct actactaatc tggttaaaat
      421 ttatggatat gtaggcaagc cggaatatgc tcaaaaatcg gcaaatcagt attttttcgt
      481 caatggacgt tatatgcggc atccctactt tcataaggca gttttaaccg cttatgaaca
      541 aatgattcac gaaaacgaga gtccaaatta ttttatctat tttgaaattg atccaaaaaa
      601 tattgatgtc aatattcatc cttccaaaac agaaatcaaa tttgagaatg agcaagccat
      661 ctggtctatc cttttagcta ccattaaaga agcactcggc aaatttaata ttattccttc
      721 tattgatttt aatagcgaag gtgatgttaa acttcctcct aaatcgtcaa tggggaatgt
      781 agttccacct cagccatctt ttaatcctta ttttaatcct tttggggata ccacttatca
      841 gcgaacacct cttaactggg aagaacttta taaaggattc gaaaaaaaca aggggatgac
      901 cattgaaaaa caatctccag atgaaacttt gtttgattct atgaaatact cctccgacga
      961 gaagggaaaa atttttgaca ataatttgtt agcaacttct tattttcaat tgaagaatca
     1021 ctatgtttta acgggtatca aatctggttt attgataatt gaccaacata aagctcactt
     1081 gaggattttg tttgagactt ttttaaacca gatggaactt caaaaaggtt tttctcagca
     1141 attgcttttt cctgagacat taaaattaac agtaaatgat gcattgtttt tcgaaactat
     1201 tattgagcca ttgaaaaaaa caggatttat tattgagaat aaaggaaata atatgtatga
     1261 gattaaagga ataccagcat ctttagaatc ggttaatatt atagaacttt tacgtgattt
     1321 aattgaaaag agtaaaacaa ctgaggaaga tccctcttta cacataaagg aaacgatcgc
     1381 tcttacgctt tcaaaagcag catcgttgaa agtaggacag gctttaacag ccgaagaaat
     1441 gtc
//
LOCUS       DAOTKS010000396         1431 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_73481_length_1431_cov_4.797238, whole genome shotgun sequence.
ACCESSION   DAOTKS010000396 DAOTKS010000000
VERSION     DAOTKS010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73481_length_1431_cov_4.797238"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="P5236_06825"
     CDS             <1..587
                     /locus_tag="P5236_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU64111.1"
                     /translation="IKVGKEVCLRILNEMKNPEAGLPTELIHPKTIERYFQYYFYQRA
                     SQMSYPVDIGRDDNLLRILSTNDNAVSEHVRMLGSVPEIFFRQSFQTAAEKFKSIDAP
                     TIGIVVPYHDEGKKIISDLFSQFAFEKQYELLRKAQRFTVNVFQNVFLKLVKDKLIKE
                     VPEIGVYVLSDERYYHPKFGLNTEICQDYDTLIQ"
     gene            590..1336
                     /gene="cas5c"
                     /locus_tag="P5236_06830"
     CDS             590..1336
                     /gene="cas5c"
                     /locus_tag="P5236_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HRU64112.1"
                     /translation="METPKNSIQFKVWGKYALFTDPITKIGGERCSYHVPTYEALKGI
                     AKSIYWKPTFIWYIDKVRVMKPVRTQTKGMKPIKMTGGNDLAIYTYLVDVEYQVLAHF
                     EWNEYQTGLAKDRIDGKHYQIAHRMLERGGRQDIFLGTRDCQGYVEPCEFGEGEGFYD
                     DIDEVAFGLMFHGFDYPDETGQNELVTRLWNPVMKHGIIEFYRPEACTIRKFVRQMQA
                     KRFGLDTNLKDVETEYNEIIFNAGKEDGIL"
BASE COUNT          502 a          231 c          294 g          404 t
ORIGIN      
        1 atattaaagt agggaaagaa gtgtgcttgc gaattctaaa tgaaatgaaa aatccggaag
       61 ccggactccc aactgaatta attcatccta aaacaattga acgttatttt caatattact
      121 tttatcaaag ggcatctcaa atgagttacc cagtggatat tggacgtgat gataacctgc
      181 tcagaatttt aagcacaaat gataatgctg taagcgaaca tgtaagaatg ttaggttctg
      241 tacctgaaat cttttttcgg caatcgttcc agactgcagc tgaaaagttt aaatctatcg
      301 acgcaccaac aattggaata gttgtacctt atcatgatga aggcaaaaaa ataatttcag
      361 atcttttctc tcaatttgct ttcgaaaagc agtatgaatt attacgcaaa gctcagcgtt
      421 ttacagtgaa tgttttccag aatgtttttt taaaattggt gaaagataaa ctcataaaag
      481 aagtgcccga aataggagta tatgtcttaa gtgatgaaag atattaccat cctaaatttg
      541 gtttaaatac tgaaatatgc caagattatg acactttaat tcaataaata tggaaacacc
      601 aaaaaattca attcagttca aggtttgggg taaatatgct ctatttaccg atcccataac
      661 caaaatagga ggggaacgtt gctcttacca tgtaccaact tacgaagcac tgaagggaat
      721 tgccaaatcg atatattgga agcccacctt tatttggtat attgataaag tcagggtgat
      781 gaaaccagtt agaactcaaa caaaaggaat gaagccaata aaaatgacag gtgggaatga
      841 ccttgctata tacacatatc ttgtagacgt tgaataccag gttttggctc attttgaatg
      901 gaacgaatat caaactggac ttgcaaagga tcggatagat ggcaagcatt accagattgc
      961 tcatcgaatg ctcgaacgtg gtggccgaca ggatattttc cttggtacaa gagattgtca
     1021 gggttacgtt gaaccttgtg aatttggaga gggggaaggt ttttatgatg atattgacga
     1081 agtagctttt ggccttatgt tccatggatt cgactaccct gatgagacag gccagaatga
     1141 acttgtaacc cgattatgga acccggtaat gaaacatgga atcattgaat tttacaggcc
     1201 ggaagcgtgt actatcagga aatttgttcg ccaaatgcaa gccaaaagat ttggcttaga
     1261 tacaaatcta aaagatgtag aaactgaata taacgaaatc atttttaacg ccggaaagga
     1321 ggacggcata ttatgagctg gatgaaagaa ttatataaaa cgtatgaaaa ttgcataaat
     1381 aaggttgatc ttgaagctaa ccccgaaaaa gcagaaaaac ctttattgcc g
//
LOCUS       DAOTKS010000397         1427 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_73825_length_1427_cov_3.014577, whole genome shotgun sequence.
ACCESSION   DAOTKS010000397 DAOTKS010000000
VERSION     DAOTKS010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73825_length_1427_cov_3.014577"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /gene="argC"
                     /locus_tag="P5236_06835"
     CDS             <1..922
                     /gene="argC"
                     /locus_tag="P5236_06835"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRU64113.1"
                     /translation="ELIRILIYHPEVEIIFIHSKSKAGKQVTEIHSGLIGETDLQFTS
                     ELPFEKIEVLFLCLAHGESKQFIETNKIPTEIKLIDFSADFRLDMHSNFVYGLPELQA
                     EKIKKAIHIANPGCFATAIQLALLPLAKNGLITNEIHVSGITGSTGAGVKPSETSHFS
                     WRNNNVSVYKAFTHQHLQEIQQTLHQLQPSFNQEINFIPMRGNFTRGILVSCYTHCSL
                     NENDAKGLYKKFYEKSAFTFVTDKNPDLKQVVNTNKALVYLEKYNEKLFIVCVIDNLL
                     KGASGQAVQNMNLMCGLDEKTGLQLKAIGF"
BASE COUNT          533 a          205 c          229 g          460 t
ORIGIN      
        1 tgaattaatt cgtattttaa tttatcatcc tgaagttgaa attatcttta ttcatagcaa
       61 aagtaaggca ggaaaacaag taactgaaat acacagcggc ttgattggtg aaactgacct
      121 gcaatttacc agcgaattac catttgaaaa aatagaagtt ttatttttat gcctggcaca
      181 cggtgagagt aaacaattta ttgaaactaa taaaattcct actgaaataa aactgattga
      241 tttctctgct gattttcgtc ttgatatgca ttctaatttt gtgtatggat tgcctgaact
      301 tcaagctgaa aaaataaaaa aagctattca tatagccaat ccagggtgct ttgctactgc
      361 tattcaactg gcattgttac ctttggcaaa aaatggtttg attactaatg aaattcacgt
      421 atcgggaatt actggctcca ctggagctgg agtaaaaccc tcagaaacaa gtcattttag
      481 ctggcgtaat aacaatgttt ctgtttataa agcttttact catcaacatt tgcaggaaat
      541 tcagcaaacg cttcatcaac tgcaaccttc tttcaatcaa gagattaact ttattccaat
      601 gagaggcaac tttactcgtg gaattttagt ctcttgttat acccattgct cattgaatga
      661 aaatgatgca aaaggattat acaaaaagtt ttacgaaaaa agtgctttta cttttgtaac
      721 agataaaaac ccagatttga aacaggttgt aaatactaat aaagcacttg tttatttaga
      781 aaaatacaac gaaaaattat tcattgtttg tgtgattgat aatttgttaa aaggtgcttc
      841 agggcaggca gtccagaata tgaaccttat gtgtggactt gatgaaaaaa caggcttaca
      901 attgaaagca atcggatttt gaaggaaaat tgtaattaaa tcagtttaag ttatattttt
      961 tgtaagcttt tcggaaaata aattgaattg aattgaattg aattgaattg ttcttttcta
     1021 aataattgtt aaaactaaga gcaaatttta tcaaagtaaa taatttgaac ttggcaaaaa
     1081 tcgcttcaga taaagtctat aataaagaca aaagctatcc cgacgcaagc atcggggaat
     1141 tcttacgatt aaaattattt ttagtgagaa agtttcacca cgttgttgtt ctttccctcg
     1201 tgataaagtg aaatataatc gaatgaaaca ataaaaaaca ttcgaacaat aacctcatta
     1261 attttaaaat tatgaaaata ggtataacag cagatctaca tttgaaatca ataaatgaaa
     1321 ctccagaaag atacgatgca atagaaaaaa ttataaacta tttaaacgat agtaacatta
     1381 atcatctaat cattgctgaa gatttgtttg ataaagaaag atacaat
//
LOCUS       DAOTKS010000398         1412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_74963_length_1412_cov_4.237288, whole genome shotgun sequence.
ACCESSION   DAOTKS010000398 DAOTKS010000000
VERSION     DAOTKS010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74963_length_1412_cov_4.237288"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="P5236_06840"
     CDS             <1..684
                     /locus_tag="P5236_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRU64114.1"
                     /translation="IKMSMHWVQIDNERKELEKAKTEAELQNLKNQVNPHFLLNTLNN
                     IYALINFNPQQAQQAVQDLSKLLRYILYDNNQTFVPLSQEVEFMQNYINLMRIRLPEN
                     VELITDFELLPDSNINIAPLIFISLIENAFKHGISADKPSFIHIQLIENTEGLLQFTC
                     ENSYFPKSESDKSGKGIGLQQLQKRLDLLYPNRYIWKKEIINNVYSTLLIINTNNEPE
                     DDTQLLDHR"
     gene            656..1366
                     /locus_tag="P5236_06845"
     CDS             656..1366
                     /locus_tag="P5236_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRU64115.1"
                     /translation="MILNCWIIDDEPLAINLLESYVEKTPFLKLTGKYSSALAAMHDL
                     ENQQPNIIFLDIQMPELSGMEFSQFVHPEARIIFTTAFSEYAINGYKVNALDYLLKPI
                     SFTDFLTAAKKALNWFEISQKAAQNEGTEKTGIFVKADYKLVQVLFDDILFVEGLKDY
                     VKIYVRNDTKPIITLMSMKGMEDLLPRDRFLRVHRSYIVQKNKIENISKNRITIAGHK
                     ILIGNTYRQNLMDEIGNI"
BASE COUNT          503 a          259 c          230 g          420 t
ORIGIN      
        1 attaaaatga gtatgcattg ggtacagata gataacgaac gaaaagagct ggaaaaagct
       61 aaaaccgaag cggaacttca aaaccttaaa aatcaggtta atccacattt tttgctcaat
      121 actttaaata atatttatgc actcatcaat tttaatccac aacaagctca acaggcagta
      181 caagatttaa gtaaattatt aagatatatt ctgtatgata acaatcaaac ttttgtacct
      241 ttgtcacaag aagttgaatt tatgcaaaac tacatcaatt taatgcgaat aaggctgcca
      301 gaaaatgttg aattgattac tgactttgaa ttattaccag atagtaatat taacattgct
      361 cctttaattt ttatttcttt gatagaaaat gctttcaaac atggaatcag tgctgacaaa
      421 ccttcgttta ttcatattca attaattgaa aacacagaag gattattgca atttacctgt
      481 gaaaacagtt atttccccaa gtctgaatca gacaaaagtg gtaaaggcat aggattgcag
      541 caattacaaa aaagattaga ccttctttat ccgaatcgtt atatctggaa aaaggaaatt
      601 attaacaatg tctattctac tcttttgatt atcaacacca ataatgaacc tgaagatgat
      661 actcaactgc tggatcatcg atgacgaacc acttgccatc aatctgctgg aatcgtatgt
      721 agaaaaaaca ccttttctga aactgacagg caaatattcg agtgccttag cggctatgca
      781 cgatttggaa aatcagcaac ctaacattat ttttctggat attcaaatgc ccgaactcag
      841 cggcatggaa ttttcacagt ttgttcatcc cgaagcaaga attattttta ctaccgcttt
      901 cagtgaatat gccattaacg gttacaaggt aaacgccctt gattatttgc ttaaaccgat
      961 ttcatttacc gactttctta cggctgccaa aaaagccttg aactggtttg aaatttccca
     1021 aaaagctgct caaaacgaag gaacagaaaa aacgggaatt tttgtaaaag ccgattacaa
     1081 gttggtgcaa gtgcttttcg acgatatcct tttcgtggaa ggattaaaag attatgtgaa
     1141 aatctatgtc agaaacgata ccaaacccat cattacgcta atgagcatga aaggcatgga
     1201 agatttgctt ccacgcgaca gatttcttcg cgttcaccgc tcctatattg ttcaaaaaaa
     1261 caaaatagaa aacatcagca aaaaccgaat cacgattgcc gggcataaaa ttcttatcgg
     1321 caatacctac cgacagaatt taatggacga gataggaaat atttgataaa tcgatacagg
     1381 ttctgtttac gtctttctac aaaatttttt ct
//
LOCUS       DAOTKS010000399         1407 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_75387_length_1407_cov_3.309911, whole genome shotgun sequence.
ACCESSION   DAOTKS010000399 DAOTKS010000000
VERSION     DAOTKS010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75387_length_1407_cov_3.309911"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1162
                     /locus_tag="P5236_06850"
     CDS             <1..1162
                     /locus_tag="P5236_06850"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HRU64116.1"
                     /translation="IKSQKGSMLATGNVQLIPPLNNLKINDAGMKVEFNNFEPISHKY
                     YNMELSGNASLATNQDSSYFSGEVTIPRSEFYLPAVMRLIGQTSVPEMPKPLLVEEIE
                     KMQAGDSLIIKLRPDTSKQVSKKSSVMLPNLQGNIRVKIPHNTWIKNDNMRLELSGDV
                     EFMKHYNFFELFGSVEVVRGQYNLLGKVFVVESGTVTFAGGEKINPILDLKASYTFHD
                     QARAQRKLVIQVTGETSLPEIAFTLDDEPISEGDAVSYILFGTQLDAISFDQQASINE
                     GLSSSGLAKTAIGSFVSSEITKLLGSAFNVDYVEFKSKGSLSNGSFVVGKYLTNNLFL
                     SYERNFGNFIKEENVSEYEVKVEYELFHFLFLQLTSSPLRNGVDIVFKINSK"
     gene            complement(1307..>1407)
                     /locus_tag="P5236_06855"
     CDS             complement(1307..>1407)
                     /locus_tag="P5236_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HRU64117.1"
                     /translation="LRKLLKDDPNVAIINIHGKGYKLIAPQPKDKN"
BASE COUNT          458 a          218 c          270 g          461 t
ORIGIN      
        1 gataaaaagt cagaaaggtt ccatgctggc aactggaaat gttcagttga ttccgccact
       61 gaataattta aaaataaatg atgctggaat gaaagtggaa tttaacaatt ttgagccgat
      121 cagtcataaa tattacaaca tggaattatc aggaaatgct tctcttgcca ccaatcagga
      181 tagttcttat ttttcaggtg aagttaccat tccacgttca gaattttatt tgcctgctgt
      241 gatgcgatta atcggtcaaa cttctgttcc tgaaatgccc aaacctttgt tggttgaaga
      301 aattgaaaaa atgcaagcgg gcgattccct gatcattaaa ttaagaccag acacttcaaa
      361 acaagtgtca aaaaaaagta gcgtgatgct tcccaattta caaggaaata tacgagtaaa
      421 aattccgcac aatacctgga taaaaaatga caacatgcgt cttgagttat cgggtgatgt
      481 ggaatttatg aaacattaca atttcttcga actttttggt tctgtagaag tggtaagagg
      541 acaatataat ttgttaggaa aagtttttgt agtggaatca gggaccgtta cctttgcagg
      601 tggagaaaaa attaatccca tcctggattt aaaagcaagt tatacctttc atgatcaggc
      661 aagagcacaa cgaaaattgg tgattcaagt aacgggtgaa acttctttgc cagaaattgc
      721 ttttacactt gatgacgaac ctattagtga aggcgatgct gtttcctata ttttgttcgg
      781 tacacaactc gatgctattt cctttgatca acaagcaagc ataaacgaag gactgagtag
      841 ctcaggcttg gctaaaacag caattggctc tttcgtctct tctgaaatca ctaaattatt
      901 gggaagtgcg tttaatgtag attatgtaga attcaagagt aaaggttccc tgagtaacgg
      961 ctcatttgta gttggaaaat atcttaccaa taatttgttt ttgagctatg aacgaaattt
     1021 cggtaatttc atcaaagaag aaaatgtaag tgaatacgag gtaaaagtcg aatatgaact
     1081 gtttcatttt ctttttctac aattaaccag ttctccttta cgaaatggtg tagatattgt
     1141 ttttaagata aattcaaaat agattaaaag atgcaataat ttcttcttgt tttttagata
     1201 atattaaggt ggatattttt gttttagttt tttgaaaaaa cccttattac aaagcgagga
     1261 aatcctttgt ggaattccct cgcttgttaa agaaaaattt gataatttaa tttttgtctt
     1321 ttggctgcgg tgcaattaat ttgtagcctt tcccatgaat gttaatgatg gctacattcg
     1381 gatcgtcttt caacagttta cgaagct
//
LOCUS       DAOTKS010000400         1405 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_75542_length_1405_cov_4.000741, whole genome shotgun sequence.
ACCESSION   DAOTKS010000400 DAOTKS010000000
VERSION     DAOTKS010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75542_length_1405_cov_4.000741"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1405)
                     /locus_tag="P5236_06860"
     CDS             complement(<1..>1405)
                     /locus_tag="P5236_06860"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047334 - diphosphate-fructose-6-phosphate
                     1-phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HRU64118.1"
                     /translation="NVAIKEGEATESVADHEQIKRLFPKTYGLPLITFEEGPAKEYPK
                     VNVGVILSGGQAPGGHNVIAGLFDGLKKLNPENRLFGFLDGPSGLIDHKYTELTAEII
                     DEYRNTGGFDIIGSGRTKLELEWQFEKGAEICKKLKINAIVIIGGDDSNTNACLLAEY
                     YAQKSFGIQVIGCPKTIDGDLKNEMIETSFGFDTACKVYSELIGNIQRDASSSKKYWH
                     FIRLMGRSASHITLECALQSQPNIAIISEEIQGNNLTLNDIVENIVKVIIDRADYGLN
                     YGTVLIPEGLIEFIPGMKILISELNELLANNEDFNVLNTDDEKRQFIKGLLTPESSQL
                     YRSLPKGIARQLLLDRDPHGNVQVSLIETEKLLIEMVAKRLAQLKAEGVYKGTFLPLS
                     HFFGYEGRCAIPSNFDANYTYTLGYVAAILISERKTGYLASVRNLTAPPEEWIATGVP
                     ITMLMNMEKRNAKWKPVI"
BASE COUNT          452 a          283 c          200 g          470 t
ORIGIN      
        1 aatgactggt ttccatttag catttcgttt ttccatattc attaacatgg taatgggtac
       61 tcctgtagca atccattctt ctggcggtgc agttaaatta cgaacagaag ccaaatagcc
      121 tgtttttctt tcggagataa gaatggcagc aacgtaacca agtgtataag tataattagc
      181 atcaaaattt gatggaatag cacaacgtcc ttcgtaacca aagaaatgac ttaaaggcaa
      241 aaaagttcct ttatacactc cttcagcttt cagttgggct aaccttttgg caaccatttc
      301 aatcaataat ttttcagttt caattaagga aacctgtaca ttgccatgtg gatctctatc
      361 caacagcaat tgacgagcta ttccttttgg caaacttcta taaagttgac tgctttcagg
      421 agttaataaa cctttgataa attgacgttt ttcatcatct gtatttaaaa cattaaaatc
      481 ctcattatta gccaatagtt catttaattc actaattaat attttcattc caggaataaa
      541 ttcaatcaat ccttcgggaa ttagaacagt accataattt aaaccataat cagcacgatc
      601 gataattact ttaacgatat tttcaacaat atcgttcaaa gttaaattgt taccctgtat
      661 ttcttcagaa ataatggcta tatttggttg actttgcaaa gcacattcga gtgtaatatg
      721 agaagcagaa cgccccatta gtctaataaa atgccaatat tttttggaag aactggcatc
      781 acgctgaata tttcctatca attcagagta aactttacat gcggtatcaa aaccaaaaga
      841 agtttcaatc atttcatttt tcaaatcgcc atcaatagtt tttggacaac caattacctg
      901 aattccaaaa cttttctgag cataatattc tgctaacaaa caagcattgg tattcgaatc
      961 atcgccacca ataattacaa tagcattaat tttaagtttt ttacaaattt ctgctccttt
     1021 ttcaaattgc cattctaatt ccaatttagt tcgacccgaa ccaataatat caaaccctcc
     1081 tgtgtttctg tactcgtcaa ttatctctgc agttagttca gtatatttat ggtcaatcaa
     1141 accacttggt ccatctaaaa agccaaacaa cctattttca ggattcaatt ttttcaaacc
     1201 gtcaaataag cctgctatga cattgtgtcc gccgggtgct tgacctccag aaagtattac
     1261 gcctacatta actttcggat attcctttgc tggaccttct tcaaaagtaa tgagaggcaa
     1321 cccataagtt ttgggaaaca aacgtttaat ttgttcgtga tcagcaacag attcagtggc
     1381 ttcaccttct ttaattgcta catta
//
LOCUS       DAOTKS010000401         1405 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_75560_length_1405_cov_2.832593, whole genome shotgun sequence.
ACCESSION   DAOTKS010000401 DAOTKS010000000
VERSION     DAOTKS010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75560_length_1405_cov_2.832593"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..1349
                     /locus_tag="P5236_06865"
     CDS             135..1349
                     /locus_tag="P5236_06865"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005837268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRU64119.1"
                     /translation="MKIPFSPPYIDNDVINEVVDALQSGWITTGPKVKALEHEIKQFT
                     GTKEVLCVNSWTSGAIMMLKWWGVKEGDEVIVPAYTYCATALAVMHAGAKPVMVDSVD
                     DFNISIEAIRKAITPKTKAIIPVDIAGFPCDYNAIMKLVNEPKIKDLFTPETENQRKL
                     GRILVLNDAAHSLGAWYEKEERRTGSETDIAIFSLHAVKNITTAEGGAICLNLPEPFD
                     NTALYEELRMMSLNCQTKDAFSKSKAGGWRYDVIGFGMKVNMADVNAAIGLAQIRKYD
                     KLLKERKRVFQLYNEAFSKYDWAILPPKEKDGRETSYHLYALRIKNITEEKRDAIIDE
                     IAKQEVAVNVHFIPMPMLTFFKQQGYNIDDYPVAYDNFKMEISLPIYPQLSNDQVQYV
                     IEAVVSAYEKVK"
BASE COUNT          467 a          229 c          294 g          415 t
ORIGIN      
        1 cttattctaa attcaaaaga tatctttttg attttaattt ttacaatatg ttagattatg
       61 acgaaaaaat agattggaat aaacaaaaaa tagtggataa taaaaatttt gaaattattt
      121 attaaagaaa atttatgaaa atcccatttt ctcctcctta tattgataat gacgtgatta
      181 acgaagtggt tgatgcgttg cagtcgggct ggatcacaac tggtccgaaa gtgaaagcac
      241 tggaacatga aatcaaacag tttacaggta caaaagaagt tctctgtgtt aattcatgga
      301 cgtcgggagc catcatgatg ttgaaatggt ggggcgtgaa agaaggtgat gaggtgattg
      361 taccggctta tacttattgt gcaacggcac tagctgtgat gcatgcagga gctaaaccgg
      421 tgatggtaga ttcggtggat gattttaata tttccatcga agccattcgc aaagccatca
      481 caccaaaaac caaagccatc attcctgtgg atattgccgg ttttccttgc gactacaatg
      541 ctatcatgaa attggtgaat gagccgaaaa taaaagactt gtttactccc gaaactgaaa
      601 accaacgaaa gttgggacga atattggtgt tgaatgatgc cgcacattcg ttgggtgctt
      661 ggtatgaaaa agaggaaagg cgtaccggta gtgaaaccga cattgccata ttttcgctgc
      721 atgctgtaaa gaatattaca accgccgaag ggggtgctat ttgtttgaat ctgccagaac
      781 cttttgataa tacagctctt tatgaagaat tacgcatgat gagtttgaac tgtcagacga
      841 aagatgcttt ctcaaagagc aaagcaggag gttggcggta cgacgtaatt ggttttggaa
      901 tgaaagttaa tatggcagat gtaaatgcag ctattggact ggctcaaata cgaaaatatg
      961 ataagctact taaggaacgt aaaagagttt ttcaactata taatgaagct ttcagtaaat
     1021 atgattgggc tattcttcct cctaaagaaa aagacggacg tgaaacttcc tatcatcttt
     1081 atgctctgcg gataaagaat attactgaag aaaaacgaga tgccataata gatgaaatag
     1141 caaaacaaga agtagccgtc aatgtgcatt tcattcccat gcctatgttg acgtttttca
     1201 aacaacaagg ctacaatatt gacgattatc cagtggctta cgataatttc aaaatggaga
     1261 tttctttacc tatctatcct caactatcca atgatcaagt tcagtatgtg attgaagcag
     1321 tcgtctcagc atacgaaaaa gtcaaataag aatgacccgt ttttttgata tattctttag
     1381 cactcttggt ttgataattc tttca
//
LOCUS       DAOTKS010000402         1393 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_76550_length_1393_cov_2.287743, whole genome shotgun sequence.
ACCESSION   DAOTKS010000402 DAOTKS010000000
VERSION     DAOTKS010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76550_length_1393_cov_2.287743"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..>1393
                     /gene="yidC"
                     /locus_tag="P5236_06870"
     CDS             13..>1393
                     /gene="yidC"
                     /locus_tag="P5236_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRU64120.1"
                     /translation="MLNRPNQEMIAKQKRYNDSIALVQKAKAESEAAIHQADSILSDS
                     VASPKDSTAIISDAYGDFSVAARGEEKFFTLENEVLKLTFTNKGARIYSAELKKYRRH
                     DSLPLILFQGNESDMGFTLITNNNRILNTRDLYFEPVQTVKKDKQGNQTIIFRLKTTG
                     EGYMDIAYTLPQNNYMFSFGIKAYRMNNVVPAGTNFLEMQWSTRLRQQEQGRQFENRY
                     STIQYKFLSDDVEKLNENKNDSKQIANKLKWIAFKDQFFSNVLIADDSFTSTLLESKL
                     EDKNSPYLKFYHAEMTVPFDPSGKSATTFRMYLGPNKYNILASYDKGLNKDEKLRLHK
                     LIPLGWGIFGWINRYMIIPMFNFFSRFIGNYGIIILLMTIVIKIVIFPMTYKTYTSSA
                     KMRVLKPEIDEIYAKIPPEKAMERQKAVMDLYSKVGVSPMSGCIPALLQIPILFAMFS
                     FFPASIELRQ"
BASE COUNT          439 a          281 c          269 g          404 t
ORIGIN      
        1 gttgtctttg ccttgttgaa ccgtcccaat caggaaatga tcgcgaaaca aaaaagatac
       61 aatgattcca ttgcattggt acaaaaagct aaagcagaat cagaagctgc cattcatcaa
      121 gcagactcta ttctttccga ttccgtagca tccccaaaag actcaactgc cattattagc
      181 gatgcttatg gcgattttag tgtggctgca cgtggagaag aaaaattctt tactttagaa
      241 aacgaagtgc tgaaacttac ttttactaat aaaggtgcac gtatttactc tgccgaattg
      301 aaaaaatatc gacggcacga ctcacttcca cttattcttt ttcagggaaa tgaaagcgat
      361 atgggcttta ctcttatcac caacaacaac cgaattctga atacaaggga tctttatttt
      421 gaaccggtgc agactgtcaa aaaggacaag caaggaaatc aaacaattat tttccgcctt
      481 aaaacaaccg gtgagggata tatggatatt gcctacacgc ttcctcaaaa taattacatg
      541 ttcagttttg gtattaaagc ttaccgaatg aacaacgtcg tgcccgccgg aacaaatttt
      601 ctggaaatgc aatggtcgac gcgattgcgt cagcaggaac agggacgtca atttgaaaat
      661 cgctattcca ctatccagta caaatttctt tcggatgatg tggaaaaatt gaatgaaaac
      721 aaaaacgaca gcaagcaaat tgccaacaaa ttaaaatgga ttgcctttaa ggaccagttt
      781 ttcagtaatg tactgattgc tgacgattct tttacttcta ctttgctgga aagtaaactc
      841 gaagataaaa attcacccta tctgaaattt tatcacgccg aaatgaccgt tccatttgat
      901 ccatcaggaa agtctgcaac aactttcaga atgtatttgg gaccgaataa atacaatatt
      961 ctggccagct atgacaaagg gctcaacaag gatgaaaaat tgagacttca caagctgatt
     1021 ccactgggct ggggaatttt cggctggatc aaccggtaca tgattattcc gatgtttaac
     1081 tttttcagca ggtttatcgg gaattatggc attatcattt tattgatgac tattgtcatt
     1141 aaaattgtca ttttccccat gacgtataaa acgtatactt cgagtgccaa aatgcgtgtg
     1201 ctgaaaccgg agattgacga aatttatgcc aaaattccgc ccgaaaaagc tatggaacgt
     1261 caaaaagcgg ttatggatct ttattcgaaa gtgggtgtca gtccgatgag tggctgtatt
     1321 ccggctttgc ttcaaatacc cattcttttt gccatgttta gctttttccc tgcttctatc
     1381 gaacttcgtc agc
//
LOCUS       DAOTKS010000403         1381 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_77500_length_1381_cov_3.133484, whole genome shotgun sequence.
ACCESSION   DAOTKS010000403 DAOTKS010000000
VERSION     DAOTKS010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77500_length_1381_cov_3.133484"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1381)
                     /locus_tag="P5236_06875"
     CDS             complement(<1..>1381)
                     /locus_tag="P5236_06875"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRU64121.1"
                     /translation="QMLVSARIVFADGAILDTSDEQSKNNFIQTHADFLREIERIRDE
                     IKNDEELYSFIKKKYEIKNVTGLSLQPFVDFEDPFDIILHLMVGSEGTLGFVSEAVFK
                     TTDNCPYSASGFAYFENMYLACEAVQKLKNLPVSAVEFFDRKAIASVEDKFSNIPELK
                     RLPADGAALLIKIESDDEEILHQRIDQVENLLSNAKVLFPVHFTDQPDEYNAYWDMRS
                     GIFPTVGSTRPPGTTCLIEDVCFRMENLADATDDLHQILLRNHYEDAVIYGHALAGNL
                     HFIISQRFDNEENIHQYETMMNEVVNLVVNKYHGSLKAEHGTGRNIAPFVSKEWGEKA
                     YGFMKRIKQLFDPQGILNPEVIFNEHPRCYVENIKPLPNTHPLIDKCIECGFCEVNCV
                     SAGFTISSRQRIVVQREIARLKATKENPKRLKKLEKEFLFMGNQSCATDGLCATSCPV
                     EINVGDYVH"
BASE COUNT          441 a          278 c          213 g          449 t
ORIGIN      
        1 taatgaacat aatcgcctac attgatttct accggacaag aagtagcaca aagtccatct
       61 gtagcacaac tttgattccc catgaaaaga aattcttttt ccagtttttt aagtcgcttc
      121 ggattttctt ttgttgcttt caaacgagct atttcacgtt gaacaacaat ccgttgacga
      181 gaagagatcg taaaaccagc actgacacaa ttcacttcac aaaaaccaca ctcgatacat
      241 ttatcaatga gcggatgcgt gttgggtaat ggtttgatat tttcaacata gcaacgtgga
      301 tgttcattga aaataacttc aggatttaaa attccttgag gatcaaaaag ttgcttgatc
      361 cttttcataa aaccataagc tttttctccc cattctttgc tcacaaatgg agctatattt
      421 cttccagtgc catgctccgc tttcaaagaa ccatgatatt tgtttactac cagattcacc
      481 acttcattca tcatggtttc atactgatga atattttctt cgttatcaaa acgttgactg
      541 ataataaaat gaagattccc tgccagtgca tgaccataaa tgacggcatc ctcataatga
      601 tttctgagca atatttgatg caaatcgtca gttgcatctg ccagattttc cattctgaaa
      661 caaacatctt caatcaaaca agtagttcca ggcggacgag tactgcctac tgttgggaaa
      721 atgccagaac gcatatccca ataagcattg tattcatcag gctgatcagt aaaatgtact
      781 ggaaaaagca ctttagcgtt tgataaaaga ttttccactt gatcaattct ttgatgaaga
      841 atttcttcgt catccgattc aatttttatt aacaaagctg ctccatcagc tggcaaacgt
      901 ttaagttccg gaatattgct gaatttatct tcaactgaag caattgcttt tctatcaaaa
      961 aattctactg cactgacagg aagatttttt aatttttgaa ctgcttcaca agcaagatac
     1021 atattttcaa aataagcgaa tccactggca ctgtatggac aattatcagt tgttttgaaa
     1081 acagcttctg aaacaaaacc caatgttcct tccgaaccca ccattaaatg caaaataata
     1141 tcaaaaggat cttcaaaatc aacgaatggt tgaagtgaaa ggcctgtcac attttttatt
     1201 tcatattttt tcttgatgaa ggaatataat tcttcatcat ttttgatttc atctcttatt
     1261 cgctcaattt cccgaagaaa atcagcgtgg gtttgaataa aattattttt actttgttca
     1321 tcgcttgtat caagaatagc tccatctgca aaaacaattc gagcagaaac cagcatttga
     1381 t
//
LOCUS       DAOTKS010000404         1377 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_77828_length_1377_cov_3.245840, whole genome shotgun sequence.
ACCESSION   DAOTKS010000404 DAOTKS010000000
VERSION     DAOTKS010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77828_length_1377_cov_3.245840"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="P5236_06880"
     CDS             <1..716
                     /locus_tag="P5236_06880"
                     /inference="COORDINATES: protein motif:HMM:NF023205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3316 domain-containing protein"
                     /protein_id="HRU64122.1"
                     /translation="YLSPLTYDGIGFRYDYSSRKYLSPNNLKLSRENQFNLNIAVTEN
                     PAKTSNISYLGMDYSWGLHYHFRPAKNVQLLAGSLWDIEFGLKANSRNQNNPMNIDLA
                     TNLNFSGMAIYDTRILKYPFRFQFTFSSPFLGLMFIPEQGESYYEIFELKNYSDILHF
                     SSFHNKQGFTEELTIQVPLKHTTWKLAVRNDYLKYSANEMVFKRNDWAISLGYVFDIY
                     KFAGRKNKLPANCISPDYK"
     gene            732..>1377
                     /locus_tag="P5236_06885"
     CDS             732..>1377
                     /locus_tag="P5236_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRU64123.1"
                     /translation="MQKYLVIFLLAIFSFSCIEDPDIQPNTFDGNFKALWEIIDTRYC
                     YLDYKHINWDSVYYAYHLRVSYVKNEYELFDLMDKMLAELKDGHVNLYSPFNVSRYWN
                     WFTDYPKNFNDSLIYTDRYLGKNYRIAGGMHYEMINNGNIGYIYFGDFEKEFTDTNIA
                     YIFDYFSHCKGIIIDVRNNGGGYLSSAEQLASYFFKEKTLTGYISHKTGPGHSDF"
BASE COUNT          436 a          203 c          237 g          501 t
ORIGIN      
        1 attatctttc tccattgact tacgatggca taggattccg ttatgattat tcaagtagga
       61 aatacctttc tcctaacaac ctaaagttat cacgcgaaaa tcaattcaat ttaaatattg
      121 ccgtcaccga aaaccctgct aaaacaagta atatcagtta tttaggaatg gattatagtt
      181 ggggtttgca ttatcatttt cgtcctgcaa agaatgttca attgttagct ggcagtttgt
      241 gggatattga gttcggcttg aaagctaaca gccgaaacca aaataatcct atgaatatag
      301 atttagctac gaatttgaat ttttcaggca tggcaattta cgatacaaga attttgaaat
      361 atccttttcg ttttcaattt actttctctt ctcctttttt aggtcttatg tttatcccag
      421 aacaaggaga atcttattat gaaatttttg aattgaaaaa ttattccgat attcttcatt
      481 ttagttcttt ccataacaaa caaggtttta cagaagaatt aactattcaa gtccctttaa
      541 aacatacaac gtggaaatta gccgtcagaa atgattactt gaagtattcg gctaatgaaa
      601 tggtttttaa acgtaacgat tgggctattt cactgggcta tgtatttgat atttataaat
      661 ttgctggtag aaaaaataag ttgcctgcca attgtattag tcctgattac aaataaaaag
      721 aaagattctc gatgcaaaaa tatctggtaa ttttcttgtt ggcgattttc tctttttcat
      781 gtattgaaga tcctgatata cagcccaata cttttgacgg caacttcaag gcactatggg
      841 aaattattga tacacgatat tgttatctgg attataagca cattaattgg gattctgttt
      901 attatgccta tcatcttaga gtttcttacg ttaaaaatga atatgaattg ttcgatttga
      961 tggataaaat gcttgctgaa ttgaaagatg gacatgtgaa tttatattct cctttcaatg
     1021 tcagccgata ttggaactgg tttaccgatt atccaaaaaa ttttaatgat tctctcattt
     1081 acacggatcg ctatttagga aaaaactatc ggattgctgg aggcatgcat tatgaaatga
     1141 ttaacaatgg aaatattggc tatatttatt ttggcgattt tgaaaaagag tttactgata
     1201 cgaatatagc ctatattttt gactattttt cgcattgtaa agggataatt attgatgtaa
     1261 gaaataatgg aggtggttac ttgagtagtg ctgagcagct tgcctcttat ttttttaagg
     1321 aaaaaacatt aacaggttat atttctcata aaactggtcc cggtcattct gattttt
//
LOCUS       DAOTKS010000405         1371 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_78329_length_1371_cov_4.962766, whole genome shotgun sequence.
ACCESSION   DAOTKS010000405 DAOTKS010000000
VERSION     DAOTKS010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78329_length_1371_cov_4.962766"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..815
                     /locus_tag="P5236_06890"
     CDS             27..815
                     /locus_tag="P5236_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRU64124.1"
                     /translation="MKKSIVFFLLIVLLTSCSKVLITGRKQLLLIPDSEILAMSSESY
                     RQYLDSVPLSTDKTNTALVKKVGKNISTAVENYLRANGLASEITNFSWEFNLVKDPSK
                     NAFCMPGGKVVVYEGILPVTKTEAGLAVVMGHEIAHAVAKHSSERLSQEMLAQFGAQL
                     TDLLITNKSAVTKAGIDALYGLGVQVGVLLPYSRKQEYEADRLGLIFLAMAGYDPNEA
                     ISFWERMSAESTNTIEFLSTHPSDAKRLAKMKEYLPEAMKYYKK"
     gene            complement(957..1316)
                     /gene="folB"
                     /locus_tag="P5236_06895"
     CDS             complement(957..1316)
                     /gene="folB"
                     /locus_tag="P5236_06895"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HRU64125.1"
                     /translation="MTKISLENMEFHAFHGCLEHEQKFGNLFFVSITMEVDTSLADES
                     DQLVDTIDYQQVYNVVKEEMEKPSKLLEHVAKKIMDHLFMQFPQIESLEIKVSKFFPP
                     LGGKVEKATVQMFKSRQ"
BASE COUNT          465 a          221 c          254 g          431 t
ORIGIN      
        1 aaaaaaatta cttaaaaatt ttaaccatga aaaaaagtat tgtttttttc ttgttaatag
       61 tgttactgac ttcctgctca aaagttctta ttactggtcg aaaacaatta ttgttaattc
      121 cagactccga gattctggca atgagcagtg aaagttacag gcaatatctt gattcagtac
      181 ccctttccac tgataaaacg aacactgctc tggtgaaaaa agtaggtaaa aatatttcga
      241 ctgctgtaga aaattaccta agagccaatg gtttggcatc agaaattacc aacttttcgt
      301 gggaattcaa tttggtaaaa gatccttcca aaaatgcttt ttgcatgcct ggcggtaaag
      361 tagtagttta tgaaggaatt ttaccggtaa caaaaacaga agctggattg gctgttgtga
      421 tgggacatga aattgctcat gcagtagcta aacacagtag cgaacgatta agccaggaaa
      481 tgctggcaca atttggagcg caattgacgg atttgttaat caccaataaa tcagcagtaa
      541 caaaagctgg tatagatgct ttatatggtt taggagtgca ggtgggagtt cttttgcctt
      601 actcaagaaa acaggaatat gaagccgatc gtttaggatt gatttttttg gcaatggctg
      661 gttatgatcc caacgaagcc atttcattct gggaaagaat gtctgctgaa agcactaata
      721 ctatcgaatt tctgagtacc caccccagtg atgcgaaaag gttggctaaa atgaaagaat
      781 atttacccga agccatgaag tattacaaga agtaaaaata aagcgtaaaa acagacgaaa
      841 ttggcaagtt tcgatgtgcg aggtacagag ctacaaagaa acgaggtgca ggatgcaagt
      901 agaaacattt tgcgtttgaa aaaatcttat atttttacat caaacaaaac atacttttat
      961 tggcgacttt taaacatttg tacagttgct ttttctactt tgccacccaa tggtggaaaa
     1021 aactttgaaa cttttatttc taaggattca atttgtggaa attgcataaa taaatgatcc
     1081 ataatctttt tagcaacatg ttctaaaagt ttggagggct tttccatttc ttcttttaca
     1141 acgttatata cctgttgata atcaatcgta tctactaatt gatctgattc gtcagctaat
     1201 gaagtatcta cctccatagt tatacttacg aaaaaaagat tgccaaattt ttgttcatgc
     1261 tccaaacaac cgtgaaaagc atgaaattcc atgttttcga gggatatttt agtcatataa
     1321 tttgtttcaa aaatttatat ttttaaataa gcttaaagta cttcactttc a
//
LOCUS       DAOTKS010000406         1367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_78699_length_1367_cov_4.482470, whole genome shotgun sequence.
ACCESSION   DAOTKS010000406 DAOTKS010000000
VERSION     DAOTKS010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78699_length_1367_cov_4.482470"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1292)
                     /gene="ftsH"
                     /locus_tag="P5236_06900"
     CDS             complement(<1..1292)
                     /gene="ftsH"
                     /locus_tag="P5236_06900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRU64126.1"
                     /translation="MVNRLPNINEEYLINELINAKVDFKGHFEDNTLQNFFLNWILPF
                     VVLMLVWGYFLKKMGPKNPILEIGKNKAKIQAEKPQNPITFKDVAGIDEAVEEVKEIV
                     EFLKNPSKYTRLGGKLPKGVLLVGPPGTGKTLLAKAVAGEAGVPFFSLSGSDFVEMFV
                     GVGAARVRDLFNQAKMHAPCIIFIDEIDAIGKSRAQAGYYGGGYDERENTLNQLLVEM
                     DGFDASVGVIIMGATNRPDVLDPALLRPGRFDRQIVVDKPDMKGREDIFKVHTKNIKL
                     AKDVDLKRLAAQTPGFAGAEIANVCNEAAILAVRKNHDAVTMEDFEAAIERVIAGLEK
                     KNKLINEKERKIVAYHESGHAIVGYFTPGAEEVQKVSIVPRGIGALGYTMQMPLEDRY
                     LLSESELLGKIKGLLGGRAAEELIFGEVSTGASNDLERA"
BASE COUNT          397 a          302 c          208 g          460 t
ORIGIN      
        1 gctcgttcta aatcatttga agcaccagta gaaacctctc caaatattaa ttcttctgct
       61 gccctacctc ctaaaagtcc tttaattttt cccaacaatt cactttctga taagagataa
      121 cgatcttcca acggcatttg catagtataa cccaaagctc ctatcccacg tggtacaata
      181 gaaacttttt gaacttcttc agctcctgga gtaaaataac ctacaatggc atgtccactt
      241 tcgtgataag ccactatttt tcgttctttt tcattgatta gtttgttttt cttttccaat
      301 ccagcaatga cacgttcaat agcagcttca aaatcttcca ttgtaacagc atcgtgattt
      361 tttctcacag ccagaatagc tgcttcattg caaacattcg caatttcagc tcctgcaaaa
      421 ccgggtgttt gagcagctaa gcgttttaaa tcaacatctt tagctaattt aatatttttg
      481 gtatgaactt tgaaaatatc ttcacgacct ttcatatcag gtttatctac aacgatttgt
      541 ctgtcgaaac gccccggacg tagaagagct ggatctaaca catctgggcg atttgtagct
      601 cccataataa ttactccaac actggcatcg aaaccatcca tttcaacaag gagttgattt
      661 aaagtgttct ctcgttcatc ataaccaccg ccgtagtagc cagcttgagc tctgctttta
      721 ccgatggcat caatttcatc aatgaagatg atgcaaggtg catgcatttt agcctgatta
      781 aacaaatctc gaactctggc tgctcctact ccaacaaaca tttcaacaaa atcggaaccg
      841 ctcaaactga aaaatggtac acctgcttca ccagcaactg ctttagccaa caaagttttt
      901 cctgtcccag gaggtccaac cagtaatact cctttaggca atttgccccc caaacgggta
      961 tattttgaag gattttttaa aaattctaca atttctttta cttcttctac tgcctcatct
     1021 ataccagcta catctttaaa agtgatcgga ttttgtggtt tttcagcttg aatttttgcc
     1081 ttgttttttc caatttccaa aataggattt ttaggtccca ttttttttag aaaatatccc
     1141 catacaagca ttaaaactac aaaaggcaaa atccaattta aaaagaaatt ttgtaaggtg
     1201 ttatcttcaa aatgtccttt aaaatcgacc tttgcattta ttagttcatt gatcaaatat
     1261 tcttcattta tattaggaag acgattgacc atgaattgtc tttgaacctg ttgttcaaca
     1321 tttgaaattt gtatacgcca gggggttgtt ggtgcttgag ttattgg
//
LOCUS       DAOTKS010000407         1353 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_79933_length_1353_cov_5.452234, whole genome shotgun sequence.
ACCESSION   DAOTKS010000407 DAOTKS010000000
VERSION     DAOTKS010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79933_length_1353_cov_5.452234"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="P5236_06905"
     CDS             <1..508
                     /locus_tag="P5236_06905"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRU64127.1"
                     /translation="KIDPENGNYAITIKRINDSIVLLDYENQNLLTIRPDNNFIRPKW
                     GIYRSLNNVQDLRDEIVRFADFSIQEENSMGFTLKELKKNDLTIINESKDQKLKVSYF
                     LNKPSDVTFSCFSMNGQIVKVFSRFKNLQIGEHHADFDYDSLPGGIYVLNMKTNYGTI
                     SRKVVIRN"
     gene            593..721
                     /locus_tag="P5236_06910"
     CDS             593..721
                     /locus_tag="P5236_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU64128.1"
                     /translation="MHCEYDLGDAEKGNRKISVYPQVVFDAMKKDLRTIQKLLEEA"
     gene            780..>1353
                     /locus_tag="P5236_06915"
     CDS             780..>1353
                     /locus_tag="P5236_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU64129.1"
                     /translation="MIYNKLKIIIPSLFLFCGSFLFAQQRIPANAVINTENLALYLND
                     SVRNKLAKNGKITEADLAEYFRQKFSERFFYDWHSVPSRFELYNQLYDSESGHLNRAK
                     ELMTDFSAKTSWLLPFNHPDGEAVKPHPFQHLARQHKMVDVGFAYFYDKKNPEYLQYF
                     INQRNSLIDALHTGKYEKVEGGNGVFEVFIT"
BASE COUNT          467 a          204 c          235 g          447 t
ORIGIN      
        1 aaaaatcgat cctgaaaatg ggaattatgc catcaccatc aaaaggatta atgatagcat
       61 tgttttattg gattacgaaa atcaaaattt actaactatt cgacctgata ataattttat
      121 tcgtcccaag tggggtatat atagaagtct taataacgtt caagatttac gtgatgaaat
      181 tgttcgcttt gcagattttt caattcaaga agaaaactca atgggtttta ccttgaaaga
      241 attaaaaaaa aatgatctga ctatcatcaa tgaatcaaaa gatcaaaaat tgaaagtcag
      301 ctattttttg aataagcctt ctgatgtaac ttttagttgc ttctcaatga atggacaaat
      361 cgttaaagtt ttttcacgat tcaaaaattt acagatagga gaacatcatg ctgattttga
      421 ctatgatagt ttacctggtg gaatttatgt tttaaatatg aagaccaatt atggtacaat
      481 ttcaagaaaa gtggtaattc gaaattagct aatcagtttt gaattatctt ttataagatc
      541 tctgttatgc tatgatgaga ggtgaaaata ctgccttaat agtcctattt ccatgcattg
      601 cgaatatgat ttgggtgatg ctgaaaaagg gaacagaaaa atatcagttt atccacaggt
      661 agtctttgat gccatgaaaa aagatttgag aacgattcaa aaacttttgg aagaagcata
      721 aatataaaga ttagtacact ttttaaaata acatttttta gaatataata attaaacaat
      781 tgatatacaa taagttaaaa ataattattc cctcattatt cttattttgc ggttcatttc
      841 ttttcgctca gcaacgaata ccggcaaatg ctgttattaa tactgaaaat ttggctctat
      901 acctcaatga cagtgtaaga aacaaactgg cgaaaaacgg gaaaatcact gaagctgact
      961 tagccgaata ttttcgacaa aagttttcgg aacgcttttt ttacgattgg cattctgttc
     1021 cttcccgttt tgagttgtac aatcaattgt acgatagtga atccggacat ttgaatcgtg
     1081 ccaaggaact tatgacggac ttttcagcga aaacttcctg gctgttaccg ttcaatcatc
     1141 ccgatggcga agcagtaaaa ccacatcctt ttcagcatct tgctcgtcag cataaaatgg
     1201 tcgacgttgg atttgcctat ttttatgata aaaaaaatcc ggagtatctt cagtatttta
     1261 ttaatcaacg aaattcattg attgatgcct tgcatactgg aaaatatgaa aaagtggaag
     1321 gcggcaatgg agtttttgag gtttttatta ccg
//
LOCUS       DAOTKS010000408         1353 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_79938_length_1353_cov_4.531587, whole genome shotgun sequence.
ACCESSION   DAOTKS010000408 DAOTKS010000000
VERSION     DAOTKS010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79938_length_1353_cov_4.531587"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /locus_tag="P5236_06920"
     CDS             complement(<1..1208)
                     /locus_tag="P5236_06920"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HRU64130.1"
                     /translation="MDNNNINWKEAALKASQTLYFVNDKTIRDVLLDIAEETLKRKEE
                     ILSANQKDLSAMDRTNPMYDRLLLNEQRLENIATDICNVAALPSPLGKILMETIRPNG
                     MKISKVTVPFGVIGIIYEARPNVTFDVFSLCLKSGNVCVLKGGTDAYHSNEAIVKIIR
                     SVLQKFDLDNHIVTLLPAGREATIELLNAVGYVDLIIPRGGKGLIEFTREHSKIPVIE
                     TGAGVCHTYFDEFGEVEKGSKIIFNAKTRRVSVCNALDCLIIHERRLNDLPILCQKLA
                     EKNVIIYADAKAYTFLVGKYPISLLQKATEESFGTEFLDYKMAVKTVESIQEAIQHIS
                     TYGSKHSECVISENKENIALFDTMVDAACVYTNVSTAFTDGGQFGLGAEIGISTQKMH
                     ARGPMALNEL"
BASE COUNT          441 a          252 c          205 g          455 t
ORIGIN      
        1 catagttcat ttaaagccat tggaccacgg gcatgcattt tttgtgtgct aataccaatt
       61 tctgctccca atccaaattg acctccatca gtaaaagctg tagaaacatt ggtataaaca
      121 caagctgcat caaccattgt gtcaaaaaga gctatatttt ctttattttc gcttattaca
      181 cattcactat gctttgaacc atatgttgaa atatgctgaa tagcttcctg aatggattct
      241 accgttttaa cagccatttt gtaatccaga aattcagtac caaaactttc ctcagtagct
      301 ttttgcaaca aagagatagg atatttgcct accaaaaaag tgtaagcttt tgcatctgca
      361 taaataatca catttttttc tgctaatttt tgacacagaa taggcagatc gttcaatcgc
      421 ctttcatgaa taataaggca gtcgagagca ttacaaacgc taactcgacg cgttttggca
      481 ttaaaaataa ttttacttcc tttttctact tcgccaaatt catcaaaata agtatgacaa
      541 acgcctgcac ctgtttctat tactggaatt ttggaatgtt cgcgtgtgaa ttcgatcaac
      601 ccttttccgc cccgcggaat aattaaatct acatatccta ctgcatttag cagttcaata
      661 gtggcttcac gcccggcagg aagcaaggta acaatatgat tatccagatc aaatttttgc
      721 agtacggaac gaataatttt tactattgct tcgttggaat gatatgcatc tgtgccgcct
      781 tttaatacac aaacgtttcc tgatttaaga caaagagaaa aaacatcaaa tgtaacattg
      841 ggacgggctt cgtaaatgat tccaatcaca ccaaatggca ccgttacttt tgaaattttc
      901 atcccattag gacgaattgt ttccattaaa atttttccaa gtggagaagg taatgctgct
      961 acattacaaa tatctgtagc aatattttca agacgctgtt catttaataa aagcctgtca
     1021 tacattggat tagtccgatc cattgccgac aggtctttct ggttggctga taagatttcc
     1081 tcttttcgtt ttaaagtttc ttctgctata tccaataaaa catcacgaat ggttttatca
     1141 tttacaaaat aaagtgtctg agaagctttt agagcagctt ctttccaatt tatattgtta
     1201 ttatccataa ttttaaaaat taaaaataaa attttttgag cggattttat tttacaaaag
     1261 tatcacttaa caagaataac gacaaaatta aatcaatatt ttaaataaat taattgcttc
     1321 ttggtaattt ttggagtaaa aaataataaa gaa
//
LOCUS       DAOTKS010000409         1350 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_80197_length_1350_cov_2.885714, whole genome shotgun sequence.
ACCESSION   DAOTKS010000409 DAOTKS010000000
VERSION     DAOTKS010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80197_length_1350_cov_2.885714"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="P5236_06925"
     CDS             complement(<1..669)
                     /locus_tag="P5236_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HRU64131.1"
                     /translation="MDKYSEERTQSWRVELRNSMKPKERMKIPRAKMPELLPEYRCHT
                     MKEEVNLGLTPQMAMTEAKRCLDCAKPTCIEGCPVNIDIPTFIKNIERGNFLEATKTL
                     KKSSMLPAVCGRVCPQEKQCEAQCIYLKTKKEAVAIGYLERFAADYERESGNISVPEK
                     SSANHIKVAVVGSGPAGLSFAGDMAKMGYEVVVFEALHEIGGVLKYGIPEFRLPNSVV
                     DVEVQ"
     gene            complement(745..>1350)
                     /locus_tag="P5236_06930"
     CDS             complement(745..>1350)
                     /locus_tag="P5236_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HRU64132.1"
                     /translation="KFCTLEVGDIITDLVGPLGNPTHVEKFGTVLCACGGVGTAPMLP
                     IVESMKKAGNRVVTVLAARTKELIMLENEMRKFSDDIIIMTDDGSYGNKGLVTAGIEM
                     VIKREKVDCCVTIGPAIMMKFVSELTKKYNIPTIASLNTIMIDGTGMCGSCRVTVGGK
                     TRFVCVEGPEFDAHQVDFDEMLIRLNAYIDFEQEKLKKISL"
BASE COUNT          420 a          287 c          183 g          460 t
ORIGIN      
        1 ttgaacttca acatcaacta cagaattggg caaacgaaat tcaggaattc catattttaa
       61 tactccgcct atttcatgca atgcttcaaa aaccaccact tcataaccca tttttgccat
      121 atcaccagca aatgataatc ctgcaggacc tgaaccaaca actgctactt taatatgatt
      181 agctgacgat ttttctggta ctgaaatatt tccactttct cgttcataat cagcagcaaa
      241 acgttccaaa taaccaattg caacagcttc tttttttgtt ttcaaataaa tacattgggc
      301 ttcacattgc ttttcttgag gacaaactcg accacatacg gctggtaaca ttgatgattt
      361 ctttaaagtt tttgtagctt ccagaaaatt tcctctttca atatttttaa taaacgttgg
      421 tatgtcaatg ttaaccggac aaccttcaat gcacgtgggt tttgcacaat caagacaacg
      481 tttggcttcc gtcatagcca tttgaggagt taatcctaaa ttaacttctt ctttcattgt
      541 atgacagcga tattcaggaa gtaattcagg catttttgct cttggaattt tcatgcgttc
      601 tttcggcttc atcgaattac gcaattcaac ccgccacgat tgtgttcttt cttcgctata
      661 tttgtccatt atttaggtag tattttatta tcgattttgg tttcaaataa tttgttaaaa
      721 aaaattctaa aaaatttcac atagtcagag agatattttt tttaattttt cttgttcgaa
      781 atctatatag gcatttaaac gtatcagcat ttcatcaaaa tcaacttgat gagcatcaaa
      841 ttcaggacct tctacacaaa caaatcttgt ttttccaccc actgttacac gacatgatcc
      901 acacatccct gtgccatcaa tcataatagt atttagagag gcaattgtag gaatattata
      961 tttttttgtt aattcactga caaatttcat cataatagcc ggaccaatag taacgcaaca
     1021 atcaactttt tcacgtttta taaccatttc tattcctgct gttacaagac ctttattgcc
     1081 ataagatccg tcgtcagtca taataatgat atcatcagaa aactttctca tttcgttttc
     1141 caacataatc aattctttgg tacgggcagc caaaacagta actactcgat tcccagcctt
     1201 tttcattgat tccacaatag gaagcatagg agcagtacca acaccgccac aagcacaaag
     1261 aacagttcca aatttttcta cgtgagttgg atttcccaaa ggtcctacta aatcagtaat
     1321 tatatcacct acttctaaag tacaaaactt
//
LOCUS       DAOTKS010000410         1337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_81356_length_1337_cov_3.753510, whole genome shotgun sequence.
ACCESSION   DAOTKS010000410 DAOTKS010000000
VERSION     DAOTKS010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81356_length_1337_cov_3.753510"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /gene="rpoB"
                     /locus_tag="P5236_06935"
     CDS             <1..836
                     /gene="rpoB"
                     /locus_tag="P5236_06935"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152451.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRU64133.1"
                     /translation="LVSQVILNYLKKYKELDAQLKRQKFNIAIGDELPSGIMQMAKVY
                     IAKKRKVQVGDKLAGRHGNKGIISRIVREEDMPFLEDGTPVDIVLNPLGVPSRMNLGQ
                     IFETILGWAGKELGVKFATPIFDGATMDDLNKWTDKAGLPRGGKTYLYDGGTGERFDQ
                     PATVGVIYMMKLGHMVEDKMHARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWALEAF
                     GAAHTLREILTVKSDDVNGRARIYEAIVKGDPMPTPGIPESLNVLLHELRGLCLSINL
                     E"
     gene            903..>1337
                     /locus_tag="P5236_06940"
     CDS             903..>1337
                     /locus_tag="P5236_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRU64134.1"
                     /translation="MAFKTDNKIKSNFTKISIGLASPEEILKLSHGEVLKPETINYRT
                     YKPERDGLFCERIFGPVKDYECHCGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHIQ
                     LVVPVAHIWYFRSIPNKIGYLLGMPTKKLDAIIYYEKYVIIQG"
BASE COUNT          448 a          196 c          296 g          397 t
ORIGIN      
        1 aactggtttc acaggttatt ctcaattatt tgaaaaaata taaggaatta gatgcccagt
       61 taaagcgtca gaagtttaat attgcaatag gcgatgaact cccaagtgga attatgcaaa
      121 tggcaaaagt ttatattgct aaaaaacgaa aagtccaggt aggagataaa cttgctgggc
      181 gacatggaaa caagggaatc atttcccgta ttgtacgaga ggaagacatg cctttcttgg
      241 aagatggaac accagtagat atagttttaa atcctcttgg ggtgccttct cgtatgaatc
      301 tcggacaaat ttttgaaact attttaggtt gggctggaaa agaattagga gtaaagtttg
      361 caacaccaat ttttgatgga gcaacaatgg atgatttgaa taagtggact gataaagctg
      421 gattaccacg agggggaaaa acttatcttt acgatggtgg aacaggtgaa cgatttgatc
      481 aacccgctac agtaggcgtt atttatatga tgaagttagg tcacatggtt gaggataaga
      541 tgcatgcacg ttctatcgga ccttattcac tcataactca acaaccgtta ggcggaaaag
      601 cacaatttgg tggacaacga tttggagaaa tggaagtttg ggcattagaa gcatttggtg
      661 ctgctcatac gcttcgagaa attttaacgg tgaaatcgga tgatgtgaat ggtagagcac
      721 gtatatatga agcaattgta aagggagatc cgatgcctac accaggcatt ccagaatctt
      781 taaatgtatt gttgcacgag ctacgtggtt tgtgtttaag cataaatttg gaataaattc
      841 tcagtaacaa aaagctgagg aaaagttagt ttcaacaatt ataaattgta atttttaaaa
      901 atatggcttt taaaaccgac aataaaataa aaagtaattt tactaaaatt tctattgggt
      961 tagcttcacc tgaagaaatt ttaaaattat ctcatggcga ggtattaaaa cccgagacca
     1021 tcaattaccg tacttataag cctgaacgtg atggcttatt ttgcgaacgt atttttggtc
     1081 ctgtaaagga ttacgaatgc cattgtggta aatacaagcg tatccgctat aaaggtattg
     1141 tgtgtgatcg ttgtggagta gaagttactg aaaagaaagt tcgccgtgaa agaatgggac
     1201 acattcagtt agtggttccc gttgcacata tctggtattt tcgttcaata ccaaataaaa
     1261 ttggctattt gttaggaatg cctactaaaa aattggatgc catcatttat tatgaaaaat
     1321 atgtcattat tcaggga
//
LOCUS       DAOTKS010000411         1334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_81646_length_1334_cov_3.689601, whole genome shotgun sequence.
ACCESSION   DAOTKS010000411 DAOTKS010000000
VERSION     DAOTKS010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81646_length_1334_cov_3.689601"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(251..>1334)
                     /locus_tag="P5236_06945"
     CDS             complement(251..>1334)
                     /locus_tag="P5236_06945"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HRU64135.1"
                     /translation="GDIVKVFDAQNNFLGIGHYQKGSIAVKIFSFEDVEINESFWFSK
                     IQKAFYLRQSLGIIQPSSNNTYRLIHGEGDFLPGLIIDVYGDTAVMQAHSIGMHHVRH
                     ILAKAVIKTVPDVSNIYYKSMSTLPHKADISDKEGYLMGDGTKNHIILENGLKFYVDW
                     FEGQKTGFFIDQRENRSLLERYSNGKSVLNMFCYTGAFSVYALRGNARLVHSVDSSSK
                     AIEITNKNVALNFPNEIRHQSFVSEAFDYFHQFDNQSSPYNLIILDPPAFAKHQHSIQ
                     NALRGYKHLNSEALQIIQPGGILFTFSCSQAISKEQFRLAIFSASAESKRKVRILHQL
                     SQPMDHPINIYHPEGEYLKGLVLWVE"
BASE COUNT          447 a          232 c          214 g          441 t
ORIGIN      
        1 atagcgtgat aataggactt caaatgcctt attattgcct tctttataca attttaccaa
       61 tttatcatcg gtaagttggt gcaaagattc cattatttct attttattga ttaggagtaa
      121 tttttagcaa taggcattaa atttaaagtt aaacaattga gtgtgataaa gagttgcaaa
      181 tataattatt gtttttaaaa aaacaaattt ttttcgtcga gaatttgaaa aaactacttt
      241 ttctcaagct ttattctacc cataacacca aacctttcaa atattctcct tcgggatgat
      301 aaatattgat ggggtgatcc ataggttgtg aaagttgatg caagatgcgt accttacgtt
      361 tactttcagc agatgcactg aaaatagcca gcctgaattg ttctttacta atggcttgag
      421 agcaggaaaa agtaaaaagg ataccacccg gttgaattat ttgtaaggct tccgaattta
      481 aatgtttata acctcttagg gcattttgaa tggaatgctg atgtttggca aaagctggcg
      541 gatcgagaat aattaaatta taaggacttg actgattatc aaactgatga aaataatcga
      601 aagcctcact aacgaaagat tgatgacgaa tttcgttcgg aaaattcaga gccacatttt
      661 tattggtgat ttcaattgct tttgacgaac tatccactga atgaaccaat cgcgcatttc
      721 ctcttaaagc atacaccgaa aatgctcccg tatagcagaa catattcaat acagattttc
      781 catttgaata acgttccagc aaagaacgat tttctcgttg atcaataaaa aaacctgttt
      841 tttgtccttc aaaccaatct acataaaact tcaaaccatt ttccagtata atgtgatttt
      901 ttgtcccgtc tcccattaaa taaccttctt tatcagaaat atctgcttta tgaggtaaag
      961 ttgacattga tttgtaatag atatttgaaa catcaggaac cgttttgatt acagcttttg
     1021 ccaaaatatg acgaacatga tgcatcccga tagaatgtgc ttgcataaca gccgtatcac
     1081 cataaacgtc aatgattagt cctggcagaa aatcgccttc gccatgaatc aaacgatagg
     1141 tattattact ggaaggttga atgatcccca atgattggcg taaataaaaa gctttttgaa
     1201 tttttgaaaa ccagaatgat tcatttattt caacatcttc aaacgaaaat atttttactg
     1261 caatactgcc tttctgataa tgtccgatac ctaaaaaatt attttgagca tcaaaaacct
     1321 tcactatgtc gcct
//
LOCUS       DAOTKS010000412         1294 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_85462_length_1294_cov_2.995964, whole genome shotgun sequence.
ACCESSION   DAOTKS010000412 DAOTKS010000000
VERSION     DAOTKS010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85462_length_1294_cov_2.995964"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="P5236_06950"
     CDS             complement(<1..828)
                     /locus_tag="P5236_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRU64136.1"
                     /translation="MSKRQKNSLFFLLIFITLAFFVLDLAWGSIRIPFNEIFSILAGK
                     GTNDIHTEIILNFRLPKAITAIIAGISLSIAGLMMQTLFRNPLADPYILGISSGASLG
                     AAMVMMAASLFPAVFISSNWSLIIAAVIGASLVLLLVILASFKVHDVVSLLIVGIMFG
                     TIASALVNIFQNLSNPDAVKLFIMWTFGSLSAVTWHHLLVLIPVFLIGMMIAIFLQKR
                     LDVLLLGENYARSSGVSIQQTRFLIVLATGLLAGGVTAFTGPIAFVGVAIPHIARGIF
                     "
BASE COUNT          449 a          252 c          188 g          405 t
ORIGIN      
        1 aaaaattcct ctggcgatat gaggaatggc tactcccaca aaggcaatag gtccggtgaa
       61 agcagtcact cctcctgcca ataaacccgt cgccagaaca attaaaaaac gcgtttgctg
      121 aatagaaact cccgagcttc gtgcatagtt ttctcctaat agcaaaacat ccagccgttt
      181 ttgcagaaaa atagcaatca tcattccaat cagaaaaaca ggaattaata ccagcaaatg
      241 atgccaggtt actgcactca aactgccaaa agtccacata atgaaaagtt ttaccgcatc
      301 cggattgctc aaattttgaa aaatattgac cagcgccgac gcaatagtgc caaacataat
      361 tccaacaatc aacaaagaaa caacatcgtg aactttaaag gaagccaaaa taaccaacag
      421 caaaaccagc gaagcaccta taacggcagc tataattaac gaccaattgc tactgataaa
      481 aacagcagga aacaatgaag ctgccatcat taccattgct gctcccaaac ttgctcccga
      541 acttattccc aaaatgtaag gatcagccag cggatttcta aaaagtgttt gcatcattaa
      601 tccggctatg gaaagcgaaa ttccggcaat aatagctgta atagcttttg gcagacgaaa
      661 attcaaaatt atttccgtat gaatatcgtt tgttcctttg ccagctaaaa tggaaaaaat
      721 ttcattaaaa ggtatgcgaa tgcttcccca tgccagatct aaaacaaaaa aagctaatgt
      781 aatgaatatc agaagaaaga acaagctatt tttctgtcgt ttagacattt aatatagatt
      841 ttaatttgtg attacacaaa ccattcttta aaaacaattg atttaatccc tattgttggg
      901 aactcaatca gtattggttg aacaaatgta ttatcttttt tgtaattttg ttattaagat
      961 gataataaaa ttcttcaata ggaacagatg actttggagt ttttgccttt ttttttaaaa
     1021 aaattctatc ttcatttcaa tttccaactc tttttcagca agtaatttct ttaaaacgtt
     1081 gttcttcttt tccaaatttc gaatatcttt tctttcgttg taatataaat gccatatttg
     1141 cagtaagttt ctatatacct tttgtttggg ctttttcaga atcgaaactt tttctttttt
     1201 actttccgtc ccttttataa tacaaaatta gtgtttttta tttctaatcc agtccgatca
     1261 tttaagggct aataaaactt tttaataaac taaa
//
LOCUS       DAOTKS010000413         1292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_85665_length_1292_cov_3.462409, whole genome shotgun sequence.
ACCESSION   DAOTKS010000413 DAOTKS010000000
VERSION     DAOTKS010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85665_length_1292_cov_3.462409"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1292)
                     /locus_tag="P5236_06955"
     CDS             complement(<1..>1292)
                     /locus_tag="P5236_06955"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HRU64137.1"
                     /translation="TYAVVGNMNEYQVSIGETTFGGRLELMDTTATLDYGSLIYLALQ
                     RSKTAREAIQVMTELVTEYGYRSEGESFSIGDPNEIWILEMIGKGPKNKGAVWVARRI
                     PDDCVSGHANQSRITAFPLHDKNNCLYSPEVISFARKRGYFKGKDEDFSFADSYAPLN
                     YLALRICEARIWTFFRKVNPEMDAYISYIKGESSKRLPLWVKPTYKLSAQDIKNFMRD
                     QYEGTELDITKGLGAGPFNSKLRCSPLTFMVDSVEYVHERPVATQQTAFSFVAQMRNW
                     LPNYVGGILWFGVDDAASSLYVPMYCGIHQIPDCYDIHNGSLLEYSPASAFWIYNQVA
                     NFAYSKYSYMMKDIKKVQSTWENKFNELVPNVDKIAANLPEITARDFLTDFSCLQATS
                     STQAWKKLGEYLLVKYMDGNVKMEKDGKFLQNEKGIPP"
BASE COUNT          382 a          270 c          234 g          406 t
ORIGIN      
        1 aggtggaatg cctttttcat tctgaagaaa ttttccatct ttttccattt ttacattgcc
       61 atccatgtat tttaccagca aatattctcc taattttttc caggcttgtg tagaagaagt
      121 ggcttgtaaa caactgaaat ccgttaaaaa atcgcgagct gtaatttcgg gcaaattggc
      181 agcaatttta tccacattgg gaaccaattc attgaattta ttttcccatg ttgattggac
      241 tttttttata tctttcatca tataagaata cttgctgtaa gcgaaattag cgacttgatt
      301 gtaaatccag aaagctgaag cgggcgaata ttccagcagg cttccgttat gaatgtcgta
      361 gcaatcaggg atttgatgaa tgccgcaata cattggaaca tacaaggagc tggctgcatc
      421 gtccactcca aaccataaaa ttccgccaac gtagttaggg agccagtttc gcatttgtgc
      481 tacgaatgaa aaagctgttt gttgtgtagc tacaggtcgt tcgtgaacat attccacact
      541 atcgaccata aaagtcagcg ggctacaacg caatttactg ttaaaaggtc cggcacccaa
      601 acctttggta atatccagtt ctgtcccttc atattgatca cgcatgaaat tttttatatc
      661 ttgagcactt aatttgtagg taggttttac ccataacgga agtcgtttag atgattcacc
      721 tttgatgtag gaaatataag catccatttc aggatttact ttcctgaaaa acgtccagat
      781 tcttgcttca caaatccgga gagcaaggta atttaatgga gcataggaat cagcaaaact
      841 gaaatcttca tctttccctt tgaaataacc tctttttctt gcaaaagaaa taacctcagg
      901 cgaataaaga caattgtttt tatcatgaag tggaaaagca gtaatacgag attgattggc
      961 atgaccagag acgcaatcat ctggaattcg tcgagctacc cagacagcac ctttattttt
     1021 cggacctttg ccaatcattt ccaatatcca gatttcgttt ggatcgccaa tagaaaatga
     1081 ttctccttca cttcgataac catattccgt caccagttct gtcattactt gaatagcttc
     1141 acgagcagtt ttcgaccgtt gcagtgcaag ataaatcaag cttccgtaat caagtgtggc
     1201 agttgtgtcc attaattcca accgtcctcc aaaagtcgtt tcgccaattg acacctgata
     1261 ttcattcata ttgccaacaa ctgcataagt tt
//
LOCUS       DAOTKS010000414         1284 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_86525_length_1284_cov_5.200976, whole genome shotgun sequence.
ACCESSION   DAOTKS010000414 DAOTKS010000000
VERSION     DAOTKS010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86525_length_1284_cov_5.200976"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="P5236_06960"
     CDS             complement(<1..646)
                     /locus_tag="P5236_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRU64138.1"
                     /translation="MKIPFSPPYIDQDVVNEVVDALQSGWITTGPKVKALENEIKRFT
                     GTKEVLCVNSWTSGAIMMLKWWGVKEGDEVIVPAYTYCATALAVMHAGAKPVMVDSGD
                     DFNISVEAIRKAITSRTKAIIPVDIAGFPCDYNAIMKLVNEHEITAMFKPETENQRKL
                     GRILVLNDAAHSLGAWYEKGIRTGSETDIAIFSLHAVKNVTTAEGGAICLNLPEP"
     gene            complement(659..826)
                     /locus_tag="P5236_06965"
     CDS             complement(659..826)
                     /locus_tag="P5236_06965"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRU64139.1"
                     /translation="MFIKSLFDRIISLFGLVFLFPLFLIVAVLIKVFMPDGPIIFKQK
                     RVDGKEFEIIY"
     gene            complement(830..>1284)
                     /locus_tag="P5236_06970"
     CDS             complement(830..>1284)
                     /locus_tag="P5236_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU64140.1"
                     /translation="IAFVLIGDGVSKNSYMLRCKDYNLTNVFFLPPVNKIQIPKLVGN
                     FDIAYIGGVHSILHKYGTSFNKMTDYMLCKKPILFSVDEPNSVIEKVNCGIRVAAEDP
                     EAVASAVMKFYSMSNEERDEIGKRGYDYAINNLGYDQLAARFIKEIFN"
BASE COUNT          370 a          275 c          206 g          433 t
ORIGIN      
        1 aaggttcagg caagttcaga caaatggccc cgccttcggc tgttgttacg tttttaacgg
       61 catgtaatga gaatatggca atatcggttt cgcttcctgt acgtatccct ttttcgtacc
      121 aagcacccaa cgaatgagct gcatcgttca gcaccaaaat tcgtcccagt tttcgttggt
      181 tttctgtttc aggcttgaac atggctgtta tttcatgctc gttcaccaat ttcatgatag
      241 cattgtaatc gcaagggaaa cctgcaatat ccacagggat aattgctttt gttcttgaag
      301 tgatagcttt ccgtatggct tcgactgaaa tattaaaatc gtctcccgaa tctaccatca
      361 ccggtttggc acctgcatgc atcaccgcca gtgccgttgc acaatacgta tatgccggaa
      421 caatcacttc gtcgccttct ttcacgcccc accatttgag catcatgatg gctcccgacg
      481 tccacgaatt gacgcacagc acttcttttg ttccggtaaa ccgtttgatt tcgttttcca
      541 atgctttcac tttcggaccg gttgtgatcc agcccgattg caacgcatct actacttcgt
      601 taaccacatc ttgatcaatg tagggtgggg agaatggaat tttcatatag cagttctttt
      661 aataaataat ttcaaattct ttaccatcaa ctcttttctg tttgaaaatt attggtccat
      721 caggcataaa tactttaata agtacagcaa ctatgagaaa taatgggaat aaaaatacta
      781 agccaaataa tgaaattatt ctgtcaaata atgattttat gaacattttt taattaaaaa
      841 tttcttttat aaatctagca gccaattgat cgtaacctaa gttatttatt gcataatcat
      901 accctcgttt tcctatttca tctctttcct catttgacat ggagtaaaat ttcattacag
      961 cagaagcaac tgcttcagga tcttcagcag ctacccgtat tccacaattc actttttcta
     1021 taactgaatt tggttcatca actgaaaata aaatcggttt tttacataac atatagtcgg
     1081 tcattttatt gaatgatgta ccatatttat gtaatattga atgtacccca ccgatatagg
     1141 caatatcaaa gtttccgact aattttggta tttgaatttt attaaccggt ggtaaaaaaa
     1201 acacattggt caaattgtaa tctttacacc taagcatata gctattttta cttacaccat
     1261 ctccaattag tacgaatgca atct
//
LOCUS       DAOTKS010000415         1278 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_87142_length_1278_cov_4.677841, whole genome shotgun sequence.
ACCESSION   DAOTKS010000415 DAOTKS010000000
VERSION     DAOTKS010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87142_length_1278_cov_4.677841"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..1159)
                     /gene="trpS"
                     /locus_tag="P5236_06975"
     CDS             complement(164..1159)
                     /gene="trpS"
                     /locus_tag="P5236_06975"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRU64141.1"
                     /translation="MEIVLSGIRPTGKLHLGNYFGALRNFVHLQNEYDCYFFIADYHS
                     LTTHPTPHNLFGNVREVLVEYLAAGLDPEKATLYIQSDVPQVTELYLFLNMNAYIGEL
                     QRCTSFKEKVRQQPHNVNAGLLTYPTLMAADILIHKATKVPVGKDQEQHLEMTRTFAN
                     RFNRLYKVDYFPEPQAFNFGEELIKVPGLNGSGKMGKSEGEDNAIFLADEPETIRRKV
                     MKAITDAGPTVPNQPKSEAVQNLFQLLNIVSSPETVNYFEEQYNTCQIKYGELKKQLA
                     EDMIAFTAPFRERIKEISNNDEYLRKVVRMGKEKAQAHASQTVKEVREIIGFRSF"
BASE COUNT          409 a          223 c          195 g          451 t
ORIGIN      
        1 ggcttatttc agcaattttt gagttttcag tgatgagata accattcccc aagacgagcc
       61 ctgttaaaag aataattgct aataatccat gtgagaaaaa gtttttcata attttataac
      121 aattagattt tttctcaatt tctcattaaa agtataattt tatttaaaaa gatctaaaac
      181 caataatttc acgcacttct ttcaccgttt gtgaggcatg agcttgtgcc ttttcttttc
      241 ccatacgtac cactttgcgt aaatactcat cattattgga tatttcttta attcgttcac
      301 gaaaaggagc agtgaaggca atcatatctt cagccagttg tttcttcaat tcaccatatt
      361 ttatctggca ggtattatat tgttcttcaa aataattcac tgtctcgggt gaggaaacta
      421 tattaagcaa ttgaaaaaga ttttgaacag cttctgattt aggctgattt ggtacagtag
      481 gaccagcatc cgtaatagct ttcattactt ttcttcgaat tgtttcaggt tcatctgcaa
      541 gaaaaatggc attgtcttcc ccttctgatt ttcccatttt tccactgcca ttcaatcctg
      601 gaactttaat taattcttct ccaaaattaa atgcttgtgg ctcaggaaaa taatcaactt
      661 tgtacagtcg gttaaaacgg ttggcaaaag ttcgtgtcat ttccagatgc tgttcttgat
      721 cttttcccac cggcactttt gtagctttat gaataagaat atcggctgcc attaaagtag
      781 gataggttaa caatccagca tttacattat gaggttgttg acgaactttt tctttaaagg
      841 aagtacagcg ttgtaattca ccgatgtaag cattcatgtt caagaaaaga tacaattcgg
      901 tcacttgtgg aacatcgctt tgaatatata aagttgcttt ttcgggatct aaaccggctg
      961 ctaaatattc tactaaaact tcacgaacat ttccaaacaa attgtgtgga gtaggatgag
     1021 tcgtaagaga atgataatca gcaataaaaa agtaacaatc atattcattt tgaagatgaa
     1081 caaaatttct taaagcacca aaataattac ctaaatgtaa ttttcccgta ggacgaatac
     1141 cactaagtac aatttccata tcaacctaat tctctttatt aatttttctc aagataaaac
     1201 tgaaaatgca aattttttat aagatgagaa aatcaaatat ttaaatcaat tttttatttt
     1261 tctcgaattt cgtttgca
//
LOCUS       DAOTKS010000416         1268 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_88222_length_1268_cov_3.126134, whole genome shotgun sequence.
ACCESSION   DAOTKS010000416 DAOTKS010000000
VERSION     DAOTKS010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88222_length_1268_cov_3.126134"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="P5236_06980"
     CDS             <1..715
                     /locus_tag="P5236_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRU64142.1"
                     /translation="VIKDLYKFFSDKPILRGISLTLHRGENLGIMGKSGIGKSVLAKC
                     IVRLIEPDGGEIKVLGTDILQCNDNELNEIRKKVGYLFQGGALYDSMSVRENLLFPIR
                     RTQFVDKKREAEELIETSLRSVGLLEAIDKMPSELSGGMKKRIALARTLILRPEIILY
                     DEPTTGLDAVTSGEISELIIKIREEYNTSSIIITHDTKCAKMATDNIKIMNDGVFIAE
                     GTYDELSHSKEKEIRDYFI"
     gene            734..>1268
                     /locus_tag="P5236_06985"
     CDS             734..>1268
                     /locus_tag="P5236_06985"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRU64143.1"
                     /translation="MKTQKRALRLGIFIAVAIVLFIVAVYLIGSKENLFTSNTNLYAS
                     FKDVRGLSVGNNVRFAGINVGTVKDIRIISDTSVIVTFSVDDRYTKYIYKNSTVQIGQ
                     EGLMGGKIVLILPGDPQTGRVREGDFLKVTEGLDIQGMLAQAQIMLEEATGTVANLRS
                     ITGKIDSGKGDMGNLINN"
BASE COUNT          447 a          186 c          266 g          369 t
ORIGIN      
        1 tgtaataaaa gacttgtata aatttttttc tgacaaacct attttacgag gcataagtct
       61 aaccttgcat cgtggagaaa atttaggaat tatggggaaa tcaggtatcg gaaagtcggt
      121 attggcaaaa tgtattgtaa gattaataga gcccgatgga ggcgaaataa aagttttagg
      181 gacagatata ttgcagtgca atgataatga gctaaatgaa atacgtaaaa aagtgggtta
      241 cctttttcaa ggaggtgcct tatacgattc aatgagtgtt cgtgaaaatt tgcttttccc
      301 aatacgacgt acgcagtttg tggataaaaa gcgtgaagca gaagaactga ttgaaacatc
      361 gctaagaagc gttggattac tggaggcaat tgataaaatg ccatcagaac tttcaggagg
      421 tatgaaaaaa agaattgctc tggcacgcac cttgattctt cgtccagaaa tcattttgta
      481 tgatgaaccc actactggac ttgatgcagt aacctcaggc gaaataagcg aattgatcat
      541 taaaattcgt gaggaatata atacttcttc tatcatcatt acacatgata ccaagtgtgc
      601 caaaatggct accgacaaca taaaaataat gaatgacggg gtttttatag ccgaaggcac
      661 atatgacgaa ttaagtcata gcaaagaaaa agaaataaga gattatttta tttaataata
      721 aaatcaattc aatatgaaaa cacaaaaaag agccttaaga ttaggtattt ttattgcagt
      781 tgctattgtt ttattcatag tggctgttta ccttattggc agcaaagaaa atctgtttac
      841 ttcaaacacc aatttatatg cctcgtttaa ggatgttcgg ggcttgtcag taggcaataa
      901 tgtacggttt gccggtatta atgtaggaac cgtgaaagat atacgtatta tttcggatac
      961 ctctgtcatt gttacttttt cggtagatga tcgttataca aaatacattt ataaaaactc
     1021 tactgttcaa atcggacaag aaggcttaat gggtggaaaa attgttctta ttttacctgg
     1081 tgatccgcaa acgggacgcg taagagaagg tgatttttta aaagtaacag aaggacttga
     1141 tatacaaggt atgttagccc aagcacaaat catgttggaa gaagctactg gaacagtggc
     1201 aaacctacgt tctataactg gaaaaatcga tagtggaaaa ggagatatgg gaaatctcat
     1261 taacaatg
//
LOCUS       DAOTKS010000417         1267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_88333_length_1267_cov_3.563531, whole genome shotgun sequence.
ACCESSION   DAOTKS010000417 DAOTKS010000000
VERSION     DAOTKS010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88333_length_1267_cov_3.563531"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(240..>1267)
                     /locus_tag="P5236_06990"
     CDS             complement(240..>1267)
                     /locus_tag="P5236_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRU64144.1"
                     /translation="LCATSCPVEINVGDYVHYLREEQLKNKKVAQFLGKWTAEHFHLV
                     ANSLRSVLSLAYTAEKILGKRAMSVITRGLRVISGNTFPFWSPALPKPQHKIIPPPIS
                     DNPLKVVYFPSCLNQMMSVSSNDPEQTPLIEKMISFLNKAGYDVIFPKNMEKMCCGSI
                     WESKGLLSIADQKSSELEKTLLEASENGRYPILCDQSPCLFRMRQKMHQLSLYEPVEF
                     IDKFLLDKLEFYPIDEPITIHATCSTTKMGLKPTLIKIAKLCSKQVLIPEEVGCCAFA
                     GDKGFMMPELNAHALRKLRPQIEKSNVKTGYSNSRTCEIGLNTNTGIPYMSIVYLVDK
                     CTKRKEL"
BASE COUNT          414 a          233 c          193 g          427 t
ORIGIN      
        1 tcaatattgg ctactgtggc aatccagact gctcgcattt cacgctttgg ctgggcataa
       61 atcccaataa aactgagtac aatgaaaatt acaataaatg ctattttctt cattattttg
      121 tatttaaaaa atttttgaaa tatttcaaat aaagttgact aattgaactg tttctaataa
      181 attttatacg gcaaatttaa tcaagaatta aaattatttc ataaatttcc tgaacaaatt
      241 tataattctt ttcttttagt acatttatcg accagataaa caatagacat ataagggatt
      301 cctgtattgg tattcaagcc aatttcacag gtcctactgt tgctatatcc tgttttcaca
      361 ttagattttt caatctgagg acgcaatttt cgtaacgcat gagcattcaa ttccggcatc
      421 ataaaacctt tatcacccgc aaaggcacaa caaccgactt cttcaggaat caacacttgc
      481 ttgctgcaca attttgcaat tttaatcaat gttggtttta atcccatttt tgtggtagaa
      541 caagtggcat gaatagtaat cggttcatca ataggataaa actcaagttt atccaataaa
      601 aatttatcta tgaattcaac tggttcatat aaagacaatt gatgcatttt ttgccgcatc
      661 ctgaataagc aaggactttg gtcgcacaat ataggataac ggccattttc tgaagcttcg
      721 agaagagttt tttccaattc agaagatttt tgatcggcta tagaaagcaa acctttactt
      781 tcccagatac tgccacaaca cattttttcc atattttttg gaaaaataac atcataacct
      841 gctttgttaa gaaatgaaat cattttttca ataagtggag tttgttcagg atcatttgaa
      901 ctgacgctca tcatctgatt taagcaactg gggaaataaa ccactttcaa tggattatca
      961 gagatgggtg gtggaataat tttatgttga ggttttggca aagcaggtga ccaaaaagga
     1021 aaagtatttc cacttataac acgtaatcct ctcgtaataa ctgacattgc tctcttccct
     1081 aaaatcttct cagccgtgta agccaatgat aacactgacc ttaaactgtt tgccaccaaa
     1141 tgaaaatgtt ctgctgtcca ttttcctaaa aattgagcaa cttttttgtt cttaagctgt
     1201 tcttctctta agtaatgaac ataatcgcct acattgattt ctaccggaca agaagtagca
     1261 caaagtc
//
LOCUS       DAOTKS010000418         1266 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_88440_length_1266_cov_3.476466, whole genome shotgun sequence.
ACCESSION   DAOTKS010000418 DAOTKS010000000
VERSION     DAOTKS010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88440_length_1266_cov_3.476466"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="P5236_06995"
     CDS             complement(<1..658)
                     /locus_tag="P5236_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HRU64145.1"
                     /translation="MEEERFSGLPENAHRELMEGEEYHPILSPEKNYPEVNFRSVSVG
                     LLMAILFSAAAAYLGLKVGQVFEAAIPIAIIAVGLSSAAKRKNSLGENVIIQSIGASS
                     GVIVAGAIFTLPALYILQAKYPEITVSFMQVFLSSLLGGVLGILFLIPFRKYFVADMH
                     GKYPFPEATATTQVLVSGEKGGDQAKPLIRAGIIGGLYDFLIATFGSWAEVFTSRVLP
                     F"
     gene            complement(678..>1266)
                     /locus_tag="P5236_07000"
     CDS             complement(678..>1266)
                     /locus_tag="P5236_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3372 domain-containing protein"
                     /protein_id="HRU64146.1"
                     /translation="YVSCHDDLCLVDKLKKSLPPDANVKELMKFDKLAQTVVFTSQGI
                     PFIYAGEEVFRNKKGVHNTYQSPDSINQINWNYKTTYHDIFEYYKGLISLRKLHPAFR
                     LATEQNVQEYLKFIDMNTPNVIAYQLSEHANGDSWREILVILNGNRKPVEVQIPEGEW
                     NVVCHDGQINLAGISVVKTTNFVVAASSASILYQN"
BASE COUNT          438 a          262 c          202 g          364 t
ORIGIN      
        1 caaaaggcaa aacacgtgaa gtaaaaactt cagcccaact tccaaaagta gcaatgagaa
       61 aatcatacaa acctccaata atacctgcac gaatcaaggg ctttgcctga tcaccacctt
      121 tttcaccaga aaccagaact tgcgtagtag cagttgcttc agggaaagga tattttccat
      181 gcatatcagc cacaaaatat tttctgaaag gaattaaaaa caaaattccc agcactccac
      241 ccaaaagcga actaagaaaa acttgcatga aacttacggt aatttccgga tattttgcct
      301 gaagaatata caaagcaggc aaagtaaaaa ttgctcctgc aactatgaca ccagaactgg
      361 caccgatgga ttgaataata acattctcgc ccagcgaatt ttttcgttta gcagcactgg
      421 aaagtcctac agcaataatg gcaatgggaa ttgcagcctc aaacacctgt ccaactttca
      481 aacctaaata agctgccgct gctgaaaaaa gaattgccat tagcaaacca acagacacag
      541 aacgaaaatt aacttctgga taattttttt caggtgaaag aatgggatga tattcttcac
      601 cttccattaa ttcacggtga gcattttctg gcagtcctga aaatctttct tcttccatct
      661 acttttaagt caacagatta attttgatat aaaatacttg ctgaagaagc tgccactaca
      721 aaatttgttg ttttcacgac cgaaatccct gccagattga tctgtccatc gtgacatacc
      781 acattccatt caccttcagg aatttgcact tccaccggtt tcctgttacc attcagaata
      841 actagaattt cacgccagct atcgccattg gcatgttctg aaagttgata agcaataaca
      901 ttaggcgtat tcatatcaat aaatttcaaa tattcctgca cattttgttc agtagctaat
      961 ctaaaagcag gatgtaactt acgtagtgag attaaccctt tgtaatattc aaaaatatca
     1021 tgataagtag ttttgtaatt ccaattgatt tgattaatgg aatcgggtga ctgataagtg
     1081 ttatgaacac cttttttatt acggaaaact tcttcaccgg cataaataaa tggaattcct
     1141 tgcgaagtaa agacaactgt ttgagctaat ttatcaaatt tcattaattc tttcacattg
     1201 gcatcaggag gtagggattt ttttaattta tcaaccaaac ataaatcatc atgacaggaa
     1261 acataa
//
LOCUS       DAOTKS010000419         1266 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_88457_length_1266_cov_2.848885, whole genome shotgun sequence.
ACCESSION   DAOTKS010000419 DAOTKS010000000
VERSION     DAOTKS010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88457_length_1266_cov_2.848885"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1266)
                     /gene="mnmG"
                     /locus_tag="P5236_07005"
     CDS             complement(<1..>1266)
                     /gene="mnmG"
                     /locus_tag="P5236_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRU64147.1"
                     /translation="SEPASFGLTEQLVSLGLKAGRMKTGTPARIDGRTIDFSQLKEQV
                     GDNDFHKFSFLEEVNRELKQMSCWITYTNPLTHEELRKGLADSPLYNGQIKSIGPRYC
                     PSIETKIVTFAEKQSHQLFLEPEGVDSQEYYLNGFSSSLPYPIQIKAIHTIAGLENAQ
                     LYRPGYAIEYDFFDPTQLTHTLETKKIKNLFFAGQINGTTGYEEAAGQGLIAGINAHM
                     KCHGNAEFTLTRDESYIGVLIDDLVTKGVDEPYRMFTSRAEHRLLLRQDNADERLTEK
                     GYQIGLASQNRYYQHQRKKEEQAKLIQFIQHYSVKPQEVNAFLSARGLSELKHGCKLS
                     DIILRPQLNINDLGSVIPALKQKIDEIPSRREEIIESTEIILKYSGYIEREKMIAEKL
                     QRLEHLIIRGRFDYDSILSLSTEARQKLMR"
BASE COUNT          393 a          233 c          215 g          425 t
ORIGIN      
        1 cgcatcaatt tctgccgagc ttcggttgat aaagataaaa tagaatcata atcaaaacgt
       61 ccacgaatga ttaaatgttc taatcgttga agtttttctg caatcatttt ttcacgctca
      121 atatagccac tgtatttcaa aataatttca gtagattcga taatttcctc tcgtcgagag
      181 ggaatctcat caattttttg ttttaaagca ggaataacag agcccaaatc attgatgttt
      241 agttgaggcc ttaaaattat atcagataat ttacaaccat gctttaattc tgataaacct
      301 cttgcagaaa gaaaagcatt cacttcttga ggctttactg aataatgttg aataaattgt
      361 ataagttttg cttgttcttc tttttttctt tggtgctgat aatagcgatt ttgtgaagct
      421 aatccaattt gatatccttt ttcagtaaga cgttcatcag cgttatcctg acgaaggagt
      481 aaacgatgtt cagcacggga agtaaacatt cggtaaggtt catcaactcc tttggtaacc
      541 aaatcatcaa ttaaaactcc gatataagat tcatcacgag tcaaagtaaa ttcagcattg
      601 ccatgacact tcatgtgagc attgatacct gcaatcaaac cttgtcctgc agcttcttca
      661 tatcccgttg ttccattaat ttgtccagca aaaaacagat tttttatttt ctttgtttcc
      721 agtgtatgtg ttaattgggt aggatcaaaa aaatcatatt caatggcata ccccggcctg
      781 taaagttgag cattttcaag acccgcaatg gtatgaatag ctttaatttg aatgggatac
      841 ggaagtgaag aggaaaatcc attgagataa tattcctgtg aatccacccc ttccggttca
      901 agaaaaagtt gatgactttg cttttcagca aaagttacaa tttttgtttc aatactggga
      961 caataacgtg gaccaatact ttttatttgt ccattgtata aaggcgaatc cgccagtcct
     1021 ttccttaatt cctcgtgggt gagaggatta gtataagtaa tccagcaact catttgtttt
     1081 aattcacgat tcacctcttc taaaaaggaa aatttatgaa aatcattgtc gccgacttgt
     1141 tctttaagtt gagaaaaatc aatagttcga ccatcaattc tggcaggtgt tcctgttttc
     1201 attcttccag cttttaaacc caatgaaaca agttgttcag tcaaaccaaa tgaagcaggt
     1261 tctgaa
//
LOCUS       DAOTKS010000420         1254 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_89740_length_1254_cov_2.466222, whole genome shotgun sequence.
ACCESSION   DAOTKS010000420 DAOTKS010000000
VERSION     DAOTKS010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89740_length_1254_cov_2.466222"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1254
                     /locus_tag="P5236_07010"
     CDS             <1..>1254
                     /locus_tag="P5236_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRU64148.1"
                     /translation="YSSLKTATTGKFDFQVAGQQKGFGANEFYSLTYPHQYENTQTYF
                     TSLSWNLNKGKLNYLAQIYWRKHHDRFELFRNYENAPSWYQNHNYHLTDIKGAKANAN
                     YIDNWGKITLGIELRNEHIYSTVLGEPMKKTVVDPFEHNIDFTHEGKRFHSTFLMDYS
                     KDFQHWYCSTGIATTHSSSFGQNTFGGFDIAFAFTDEMRIFFSANSAIRLPTFTDLYY
                     QSATQISNPNLKPETSKNLELGIKSNQKNLQWNICTYYRAGKNIIDWIKHPDSTKWIS
                     SNLIHINAFGTDFHIDYQFIGSFLKSASFSYSYLYQDKNAEQFDSKYALDYLKHKANL
                     KLEHPFWKNLTGIWAISYFDRAGNYTAFQTGKLVNYKPYLLADFQINWAKSQYEIYSN
                     LNNLLNVHYEDYGGIAQPGFTFNVGF"
BASE COUNT          447 a          216 c          188 g          403 t
ORIGIN      
        1 tattcttcat tgaaaacagc aacaactggt aaatttgact tccaagtagc tggtcaacaa
       61 aaaggttttg gtgccaatga attttattct ttaacttatc ctcaccaata tgaaaacaca
      121 caaacttatt ttacatcact ttcctggaat ttaaacaaag ggaagctcaa ttatctggct
      181 caaatatact ggcgtaagca ccatgaccgt tttgagttat ttcgaaatta tgaaaatgca
      241 ccttcctggt accaaaatca taattatcat ctgacagaca tcaaaggagc aaaagccaat
      301 gctaactata ttgataattg gggaaaaatc acattgggta tagaactcag aaatgaacac
      361 atttacagta ccgttttggg agaaccaatg aaaaagacag ttgttgatcc atttgagcat
      421 aatattgatt tcactcatga agggaaacgt ttccattcaa catttttaat ggattattca
      481 aaagatttcc aacactggta ttgttcaaca ggtatagcaa ctactcattc ttcttcattt
      541 ggacaaaata cttttggtgg ttttgatata gctttcgctt ttactgacga aatgcgtatt
      601 ttcttttcag ctaactctgc cattcgtcta ccaactttta ccgatttata ttatcaaagt
      661 gcaacgcaaa taagcaatcc aaatctgaaa ccagaaactt caaaaaatct ggaattggga
      721 attaaatcga atcaaaaaaa tctacaatgg aatatttgta cctattatcg agcaggtaaa
      781 aatattattg actggataaa acatcccgat tcgacaaaat ggataagttc caatttaatt
      841 cacatcaatg cttttggaac agattttcac attgattatc aatttattgg ctcattcctt
      901 aaaagtgcaa gtttttcata ttcttatttg tatcaagata aaaacgccga gcaatttgat
      961 tcaaaatatg ctctggatta cctcaaacac aaagcaaact tgaaattaga acatcctttc
     1021 tggaaaaatc ttacaggaat atgggcaatc tcttattttg acagagcagg aaattatacc
     1081 gcctttcaaa cagggaaatt agtgaactat aaaccttatt tattagcaga ttttcaaatt
     1141 aattgggcaa aaagtcaata tgaaatttac tccaacctga ataatttatt aaatgttcat
     1201 tatgaagatt atggtggaat tgctcaacca ggatttactt ttaatgtagg attt
//
LOCUS       DAOTKS010000421         1247 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_90514_length_1247_cov_4.081376, whole genome shotgun sequence.
ACCESSION   DAOTKS010000421 DAOTKS010000000
VERSION     DAOTKS010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90514_length_1247_cov_4.081376"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1247
                     /locus_tag="P5236_07015"
     CDS             <1..>1247
                     /locus_tag="P5236_07015"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HRU64149.1"
                     /translation="NTDTFSHLASLKYLPRWAVLFIDIAISAISFLTAYLIASAFIKH
                     LPDRLLLSLFQRLILLLITQTGFFWLFHTYSGILRYSTMTDATKLFLAIFSNCFFLSA
                     LNFVCSLYISMPLFYYSSILIYGFIAFILLFGIRLTVRNIFNYFVETSAQIIPVMIYG
                     IDEAGLGIARMILSSVNSKKQYKLVGFIDGDGNAVNKMVMGIRVYNHDDQTIKTKVAN
                     KVNTVIVSPIKMKEINPDEDLDVFIQNHISLLTSQPMDIWNGEMPSVRQIKPLKIEDL
                     LEREPIQISFDDIALELKSKTVMVTGAAGSIGSEIVRQLCNFSPKLIVLVDNGETAIH
                     NLRIELQNFSSTQNFSFLLGDVRNKERMEYIMDLYRPDFVFHAAAYKHVPLMEDFPCE
                     SVQVNVLGTKIMADLSLKYKVSR"
BASE COUNT          350 a          208 c          227 g          462 t
ORIGIN      
        1 acaatacgga tacgttctct cacctggctt ctttaaaata tttacctcgc tgggctgttt
       61 tatttataga tattgcaata tctgccatat cttttttaac agcttatctt attgcttcgg
      121 cttttataaa acatttgcct gaccgacttc ttttatctct ttttcaacgt ttgattttat
      181 tgctcattac tcagaccggt tttttctggc tttttcatac ttattctggc atcctcagat
      241 attctacgat gactgatgcc accaaacttt ttttggcaat attcagtaat tgtttttttc
      301 tctcggcatt gaactttgtt tgttctcttt atatttcaat gccacttttt tattattcat
      361 ccattttaat ttatggtttt atagctttta ttctactttt cggtatcaga ctgactgtta
      421 gaaatatttt taattatttt gttgaaacct cggctcagat cattccagtt atgatttatg
      481 gaatcgatga agcaggattg ggtatagccc gaatgattct ttcgagcgtg aattccaaga
      541 aacaatataa attggttggc tttattgatg gggacggaaa tgctgtaaat aagatggtta
      601 tgggaattag agtttacaat cacgatgatc aaactattaa aaccaaagtt gccaataaag
      661 ttaataccgt tatcgtttca cccataaaaa tgaaagaaat caatcctgat gaggatttgg
      721 atgtttttat tcaaaatcat atttctttgc tcacttcgca gccaatggat atatggaatg
      781 gtgaaatgcc ttctgtaaga cagatcaaac cattgaaaat agaagattta ttggaaagag
      841 aacccattca aatatctttt gatgatattg ctttggagtt gaaatctaaa accgttatgg
      901 ttaccggtgc tgccggatca ataggcagtg aaattgttcg acagttgtgt aactttagtc
      961 caaaattgat cgtgctggtt gacaatggag aaacagccat tcataacttg agaattgaat
     1021 tgcagaattt ttcttcaacc caaaatttta gttttttgct gggtgatgtc cgaaacaagg
     1081 agcgaatgga gtatattatg gatttatacc gccccgattt tgtttttcat gctgcagctt
     1141 acaaacatgt tcccttgatg gaagatttcc cttgtgaaag tgttcaggta aatgtgctgg
     1201 gcaccaaaat tatggctgat ttgtcgttga aatataaagt ttcgcgg
//
LOCUS       DAOTKS010000422         1246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_90607_length_1246_cov_5.389589, whole genome shotgun sequence.
ACCESSION   DAOTKS010000422 DAOTKS010000000
VERSION     DAOTKS010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90607_length_1246_cov_5.389589"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..1231)
                     /gene="coaBC"
                     /locus_tag="P5236_07020"
     CDS             complement(26..1231)
                     /gene="coaBC"
                     /locus_tag="P5236_07020"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HRU64150.1"
                     /translation="MLKDKKILVGVTGSIAAYKTAQLIRLLVRQGAQVKVVMTPTAKE
                     FISPLTLATLAKNPILVDFFDPTSGNWNSHVDLGTWADTFLIAPATANTIAKMAAGIA
                     DNLLLTTYLSIRCPVFIAPAMDVNMFFNKITQENLYKLQQQGNFIIEPSSGELASGLE
                     GKGRMEEPEKIVDFLEDYFTPKNLTGKKILITAGPTYEKIDPVRFIGNFSSGKMGFAL
                     AEVCARQGAEVLLITGPVHLSIHHPKVQQINVTSAEEMYHAVTNHFKDMDGAILSAAV
                     ADFTPIEKSENKLKHENDNLTLELKPTPDIAASLGKMKNEKQFLIGFALETNNEEKNA
                     FDKLKKKNFDFIVLNSLNDKKAGFGYDTNKITIFYPSGEKKAFNLKPKKEVAEDIVNE
                     LIQMLNAEK"
BASE COUNT          382 a          223 c          192 g          449 t
ORIGIN      
        1 attgtttcta aaaacttaat ttttttcatt tttcagcgtt taacatttga attaattcat
       61 tgacaatatc ctcagctact tcttttttag gctttaaatt gaatgctttc ttttcgccag
      121 aaggataaaa aatcgtaatt ttattagtat cataaccgaa accagctttt ttatcattta
      181 aggaattaag gacaataaaa tcaaaatttt tctttttgag tttatcaaat gcattttttt
      241 cttcattgtt ggtttcgaga gcaaatccaa tcagaaattg cttttcattt ttcatttttc
      301 caagacttgc tgcaatatcg ggtgtgggct ttaattccag cgttaaatta tcgttttcat
      361 gctttaattt attttcggat ttttctatag gagtaaaatc tgcaacagcc gcagaaagaa
      421 tggctccatc catatcttta aaatggtttg ttacagcatg atacatttct tcggccgaag
      481 ttacattgat ttgttgaact ttaggatgat gaatagaaag atgaacagga cctgtaatta
      541 aaagaacttc cgcaccttga cgagcacaaa cttctgccag agcaaatccc atttttccag
      601 aagaaaaatt accaataaaa cgcactggat caatcttttc gtaagttgga ccagccgtaa
      661 ttagaatttt tttaccagtt aaatttttag gagtaaaata atcttccaga aaatcaacaa
      721 ttttttctgg ttcctccatc ctaccctttc cttctaatcc actcgccaat tctccactgg
      781 aaggttcgat aatgaaattt ccttgttgct gtaatttata aaggttttct tgtgttattt
      841 tattaaaaaa catgtttaca tccattgcag gagcaataaa tactggacat cttattgata
      901 aataagttgt tagtaataaa ttatcagcga ttcctgcagc catttttgca atggtatttg
      961 cagtagcagg agcaattaaa aaagtgtcag cccaagttcc taaatctacg tggctgttcc
     1021 aattgccact ggtaggatcg aaaaaatcaa ctaaaattgg atttttagca agtgttgcca
     1081 gcgtaagcgg agaaataaat tctttagctg tcggcgtcat aacaaccttt acctgtgctc
     1141 cttgcctgac taataaacga ataagctgag cagttttata agcagcaatg cttcctgtta
     1201 ctcccactaa tattttttta tctttcaaca tattagtttc atttgt
//
LOCUS       DAOTKS010000423         1243 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_90951_length_1243_cov_4.175084, whole genome shotgun sequence.
ACCESSION   DAOTKS010000423 DAOTKS010000000
VERSION     DAOTKS010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90951_length_1243_cov_4.175084"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="P5236_07025"
     CDS             complement(<1..952)
                     /locus_tag="P5236_07025"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HRU64151.1"
                     /translation="MGDNLKQKTLDALTWSAIDRFGQQIIQVIIGIIVARLLSPDDYG
                     LMGMIMIFAALSFVLVESGFGQALIRKKDASEIDFNTIFYFNILISIALYVLLYFLSP
                     AIARFFHQPQLIKIGRIIFISLLFNAFYLVPFTQKVKEMDFKSIAKINILSVFLSGSA
                     GVTLAILKFGVWALVIQQISFHFFRMIGYHFFVKWKPKKLFSFSIIKEFWSFSVHILG
                     SSILTVIFNNLYVFILGKYYQRKDVGYYSQANKLSETVNFTFQQILGSTYSMFSQIQD
                     DDERLNRIFKVMIKRTALVTIPVIFIFIVIAYSLIYVLLSA"
     gene            complement(977..>1243)
                     /locus_tag="P5236_07030"
     CDS             complement(977..>1243)
                     /locus_tag="P5236_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008775348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HRU64152.1"
                     /translation="QSVQFFIFSKEYDKIATRINEEIHRGATILEGTGWYSKQPVKVV
                     TVIARKNESQRIFRIVKEVDPDAFVSQSSVIAVYGKGFDVMKTK"
BASE COUNT          469 a          211 c          193 g          370 t
ORIGIN      
        1 ttgctgaaag cagaacataa attaaagaat aagcaattac aataaagata aatatcacag
       61 gaatagttac cagagcagtt cttttgatca ttactttaaa gatacgattc aatcgttcgt
      121 cgtcatcctg aatttgacta aacatggaat aagtacttcc caatatttgc tgaaaagtga
      181 aattgactgt ttcacttagt ttgttagctt gagaataata tccaacatct tttctttggt
      241 aatatttacc gagaatgaaa acgtataaat tattgaaaat aactgttagg atagaagaac
      301 ctaaaatatg aacactaaaa ctccagaatt ctttaataat agaaaaagaa aacaactttt
      361 taggtttcca ttttacaaaa aaatgataac ctatcattct aaaaaaatga aaagaaattt
      421 gttgaatcac caatgcccat acgccaaatt tcaaaatagc cagcgttaca ccggcacttc
      481 cacttaaaaa aacggaaaga atattgattt tagctataga tttgaaatcc atttctttca
      541 ctttttgtgt aaatggtacc agataaaaag cattgaacag caaggaaata aaaataattc
      601 ttcctatttt gattaattgt ggctgatgaa aaaagcgagc aatggcaggt gacagaaaat
      661 aaagcagaac atataatgcg atggaaataa gaatgttaaa ataaaaaatg gtattaaaat
      721 caatttcaga agcgtctttt ttacgaatca atgcttgccc aaaaccgctt tctactaaca
      781 caaatgacag ggcagcaaaa atcataatca ttcccatcag tccataatcg tcaggcgaca
      841 gtaaacgggc gacgatgatg ccaataatga cttgaatgat ttgttgacca aatctgtcaa
      901 tagcactcca ggtaagagca tcgagggttt tttgttttaa attatcaccc atttgttttg
      961 acaataaaga taagatttat tttgttttca tcacatcgaa tcctttgcca taaacggcaa
     1021 tgacagacga ttgcgaaaca aaagcatcag gatcaacttc cttaacaatt cgaaaaattc
     1081 tttgcgattc atttttgcga gcaataaccg tcactacttt aaccggttgt ttagaatacc
     1141 agccagtacc ttccaaaata gtggctccgc gatgaatttc ttcattgatt cgcgtggcaa
     1201 ttttatcata ttcttttgaa aaaataaaaa actgaaccga ttg
//
LOCUS       DAOTKS010000424         1240 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_91318_length_1240_cov_2.296203, whole genome shotgun sequence.
ACCESSION   DAOTKS010000424 DAOTKS010000000
VERSION     DAOTKS010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91318_length_1240_cov_2.296203"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..>1240)
                     /locus_tag="P5236_07035"
     CDS             complement(164..>1240)
                     /locus_tag="P5236_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU64153.1"
                     /translation="NKIIGLQIKPFELGEITESNIGEKVFQGTKQIPQELIDSGMMRL
                     YMPSAMVVEYHKYNETKYGTNQDYQPNQMFVKEYPDVKIIELKNTGTHRRLVWTLEGN
                     IKTFENIPGEMYNFRLKINQWSVDVTSQWKEGLGAILVGKKWDRSVDMDYNHQFIFCS
                     DTDLASTEFLPMEKDATTPSALFHNKLVSVPNTSLKTITDILDLEVGKTVTLKCGSDN
                     YGIKIDKSDKFSGITSAWTPGIGDTITLMKRQDGKFIELSRETAGNSALAFDANDTTP
                     SVAGGTEFIIGDNTQATAITDLEDAIEGVIYTIYGKGTTYASTIANKDTGKFALTGAI
                     TLTTGTYIKLVKASDGKFHEVARG"
BASE COUNT          321 a          254 c          204 g          461 t
ORIGIN      
        1 ataaatgtaa tagctgcttt cggtttaatt cctgttccgg cattttgtcc tggttttccc
       61 acatttactt ttatgtagcc catatctttt aatttttaaa gtttattatt agttacattt
      121 taattcagcc ggcagttcat ttctgccgac tgaattaaac ctcttatcct cgtgctactt
      181 catggaattt tccatcagaa gcttttacaa gttttatata agtgcctgta gtcagcgtta
      241 tggctccggt taatgcaaat tttcctgtat ctttattagc aatggttgaa gcataagttg
      301 tgcctttacc ataaatggta taaattactc cttcaattgc atcttccaag tcagtgattg
      361 ctgtagcctg cgtattgtcg cctataataa attcagttcc acctgctacg cttggagttg
      421 tatcattagc atcaaatgcc aatgctgaat ttccggctgt ttccctactc aattcaataa
      481 attttccatc ctgccttttc atcaatgtga tagtatctcc aattcccgga gtccaggcag
      541 atgtaattcc agagaatttg tccgacttgt caatttttat tccgtaattg tcgcttccac
      601 atttcagagt tacggttttt ccaacttcca gatcaagaat atctgtgata gtttttaaag
      661 atgtattagg aactgaaacg agtttattgt ggaataaagc acttggagtc gtagcatctt
      721 tttccattgg caaaaattcg gttgatgcca aatcagtgtc cgaacagaaa ataaattgat
      781 ggttgtaatc catatcaacg gaacgatccc attttttgcc aaccaatata gcgcccaatc
      841 cttctttcca ttgcgaagtt acatccacgc tccattgatt tattttcaaa cggaaattgt
      901 acatttcgcc aggtatgttt tcaaatgtct tgatatttcc ttcaagcgtc caaaccaaac
      961 ggcggtgtgt tcccgtattt ttcagttcaa taatctttac gtccggatat tctttcacaa
     1021 acatttggtt aggctggtag tcctggttgg taccatactt ggtttcatta tatttgtggt
     1081 attcaactac cattgcagaa ggcatgtata gacgcatcat tccgctgtca atcaattcct
     1141 gtggaatttg tttggttcct tgaaatactt tctcgcctat attactttct gttatttcac
     1201 ccaattcaaa aggtttaatt tgtaaaccta taattttatt
//
LOCUS       DAOTKS010000425         1237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_91653_length_1237_cov_3.730118, whole genome shotgun sequence.
ACCESSION   DAOTKS010000425 DAOTKS010000000
VERSION     DAOTKS010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91653_length_1237_cov_3.730118"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="P5236_07040"
     CDS             complement(<1..380)
                     /locus_tag="P5236_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU64154.1"
                     /translation="MPYRRLPNTDQARLKALKTAIEKLENQNFTHQIVPFKTVHEAKS
                     FLNIFETQLLQYRNALHNQTTASKQYQKNMATARLYISHFIQVLNLAVVRGEIKKEQK
                     QLYQLNPDSENVPALNSEKALLHWG"
     gene            complement(408..>1237)
                     /locus_tag="P5236_07045"
     CDS             complement(408..>1237)
                     /locus_tag="P5236_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRU64155.1"
                     /translation="SCNLFSENISEAIPPALGIEIFHTFTLLHDDLMDKADLRRGHLT
                     VHKKWNENIAILSGDVMQTIAFQLMTKAPSACLKKCLDLFSQTAIEVCEGQQYDMEFE
                     KRQEVLPEEYLEMIRLKTAVLLGASMKIGAWLGGAPEEDAQTLYEAGINMGIAFQLKD
                     DLLDVYGNSAEFGKKIGGDILCNKKTFLLIQALNLARGKEEKILKKWLQVDENRGEEK
                     VKAITDVYNQLGIKEICENEIQFFSDKALEKLHQISVPENKKSELLRLTRQLVLRNE"
BASE COUNT          369 a          223 c          183 g          462 t
ORIGIN      
        1 ccccaatgaa gcaaagcttt ttcactgttt aaagcaggta cattttcaga atcaggattt
       61 agttgataca attgtttttg ttcttttttt atttctccac gaacgactgc caaattcaat
      121 acttgaataa aatgtgaaat gtataaacgt gctgttgcca tatttttttg atattgttta
      181 ctggcagtgg tttggttatg caatgcattc cgatattgaa gaagttgtgt ttcaaaaata
      241 tttaaaaaac tttttgcttc atgcactgtt ttaaaaggaa caatttgatg agtaaaattc
      301 tgattttcca gcttttcaat tgcggttttt aaagctttta atcttgcttg atctgtgtta
      361 ggtaatcggc gatagggcat tttcgattga atttatttat gaaaaattta ttcgtttcta
      421 agcacaagct gacgagtcaa tcttaataat tcactttttt tattttcagg aacagaaatt
      481 tgatgaagtt tttcaagagc tttatctgaa aaaaattgaa tctcattctc acatatttct
      541 ttaattccca attgattata aacatccgtt atagctttta ctttttcttc tcccctattt
      601 tcatctactt gcaaccattt ttttaatatt ttttcttctt tacctctggc taaatttaaa
      661 gcttgaatga gtaaaaaggt ttttttgttg caaagaatat cacctccaat ttttttccca
      721 aactctgccg aattgccata aacgtccagc aaatcatctt tcaactggaa agcaattccc
      781 atgttgatgc ctgcttcata taaagtttga gcatcttctt caggagctcc tcccaaccaa
      841 gcacctattt tcatcgaggc tcccaataaa actgccgttt tgagacgaat catttcaaga
      901 tattcttcgg gaagtacttc ttgtcgtttt tcaaattcca tatcatattg ctgaccttca
      961 catacttcaa tggctgtttg tgaaaataaa tctaaacatt tttttaaaca tgctgaaggt
     1021 gctttggtca ttaattgaaa agcaatggtt tgcatcacat cgccagaaag aatagctata
     1081 ttttcattcc attttttatg cacggtaagg tgtccacgtc gcaaatctgc tttatccatc
     1141 aaatcatcat gcaacaaggt gaaagtatga aaaatttcta ttcccaaagc aggtggaata
     1201 gcctcactga tattttcaga aaataaatta catgaca
//
LOCUS       DAOTKS010000426         1236 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_91775_length_1236_cov_2.662151, whole genome shotgun sequence.
ACCESSION   DAOTKS010000426 DAOTKS010000000
VERSION     DAOTKS010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91775_length_1236_cov_2.662151"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="P5236_07050"
     CDS             complement(<1..820)
                     /locus_tag="P5236_07050"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HRU64156.1"
                     /translation="MAFINENFLKIPENYLFSEIAKRVAEHKAKNPSAKIIRMGIGDV
                     TLPLPDVVVDAMIKAAEEQRHKETFRGYGPEQGYEFLREAIAQHDFQKRGIKIEVDEI
                     FISDGAKSDTGNIGDIFHINNRVAVTDPTYPVYVDTNAMAGRAGEPDEHNQWSNLVYL
                     PCNKENNFVPALPKEKVNLIYLCYPNNPTGTTLTKEQLKVWVDYARENQAVILFDAAY
                     EAFITENNVPHSIYEIEGATEVAIEFRSFSKTAGFTGTRCGYTIIPQQVMGYTRK"
     gene            complement(883..>1236)
                     /gene="dapF"
                     /locus_tag="P5236_07055"
     CDS             complement(883..>1236)
                     /gene="dapF"
                     /locus_tag="P5236_07055"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRU64157.1"
                     /translation="PHAVIFTDNIDQLDIKTIGPIIENSSIFPKRTNVEFVEKISENI
                     LKMRVWERGSGETMACGTGACAVVVAGVLNGISSHKTTVKLLGGELEIEWNNNDNHVY
                     LTGPAEMVFEGEIDL"
BASE COUNT          378 a          252 c          181 g          425 t
ORIGIN      
        1 cttttcgagt atatcccata acttgctgtg gaataatggt ataaccgcaa cgtgtgcctg
       61 taaaaccagc cgttttggaa aaacttctga actctatagc aacttcagtt gctccttcaa
      121 tctcataaat actgtgtgga acattatttt cagtaataaa agcttcgtag gctgcatcaa
      181 aaaggataac agcttgattt tctctggcat aatctaccca aactttcaat tgttctttcg
      241 ttaaagttgt tccagtagga ttatttggat aacacaaata aattaaattt actttttctt
      301 ttggcaaagc aggaacgaaa ttattctcct tattacaagg caaatacaca agattactcc
      361 actgattgtg ctcatctggt tcgcctgctc gtcctgccat tgcattggta tcgacatata
      421 caggataagt tggatcggtc acagccaccc gattattaat atgaaaaata tcgccaatat
      481 ttccggtatc acttttggct ccatcagaaa taaatatttc atctacctca atttttattc
      541 ctcttttctg aaaatcgtgt tgagcaatag cttctcttaa aaattcatat ccttgttcgg
      601 gtccataacc tctgaaagtt tccttatgac gttgttcttc agcagctttt atcatagcat
      661 caactaccac atcgggaaga ggaagggtaa catcgccaat tcccatccga attattttgg
      721 ctgatggatt ttttgctttg tgttccgcta ctcgttttgc aatttctgaa aataaataat
      781 tttcagggat tttgagaaaa ttttcattaa tgaatgccat atttttattt tgattgaaaa
      841 attttagaaa tttttaataa taaataattg taaaacaaat aattacaaat caatttcacc
      901 ttcaaacacc atttcagcag gacccgttaa gtaaacatga ttatcattat tattccattc
      961 tatttcaagt tcaccaccta acaatttaac agtagtttta tgagaagata ttccattcag
     1021 aacgcctgcc acgactaccg cacaagctcc tgtgccacaa gccattgttt cgcctgaacc
     1081 tcgctcccac actcgcattt tcagaatatt ttccgaaatt ttttccacaa attcaacatt
     1141 tgtccttttt ggaaaaatgg aactgttttc aataatagga cctatcgttt taatatcaag
     1201 ttgatcaata ttatctgtaa aaatcacggc atgcgg
//
LOCUS       DAOTKS010000427         1229 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_92581_length_1229_cov_2.798978, whole genome shotgun sequence.
ACCESSION   DAOTKS010000427 DAOTKS010000000
VERSION     DAOTKS010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92581_length_1229_cov_2.798978"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="P5236_07060"
     CDS             complement(<1..375)
                     /locus_tag="P5236_07060"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006847857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HRU64158.1"
                     /translation="MLKIKENQDIVFMRQALIEAKEAEKKNEVPIGAVIVCGGKIIAR
                     AHNLTETLTDVTAHAEMQAITSAANYLGNKYLMDCILYVTVEPCVMCAGALGWSQIGR
                     IVYGTSDEKRGYTIFSPQALHPK"
     gene            533..>1229
                     /locus_tag="P5236_07065"
     CDS             533..>1229
                     /locus_tag="P5236_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018967644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HRU64159.1"
                     /translation="MKSKLLTLFCLFFSLIIWAVPPAGYYTSSTLDGKNTNNLRIAFQ
                     AIITNGHSVTSYDGLWNAYKTTDINPSTGKIWDIYSNNCNFTWGNDQCGNYSKECDCY
                     NREHTTPQSWFNGASPMYSDLFNVYPTDGYVNNKRSNYPYGEVGSATYTSGNGSKLGT
                     SNFSGYNGTVFEPIDEYKGDLARTYFYMATRYADQCGTWTNGNGSVVYTSTLGLTFYA
                     INLFLKWSREDPVS"
BASE COUNT          368 a          207 c          209 g          445 t
ORIGIN      
        1 tttggggtgt aaagcctgag gtgaaaaaat ggtataacct cgtttttcat cagatgttcc
       61 atacacaatt cttccaattt gtgaccagcc cagtgcccca gcacacatta cacatggttc
      121 aacagttaca tataaaatgc agtccatcaa atatttattt cctaagtaat tagctgccga
      181 agtaattgct tgcatttctg catgtgcagt gacatcagtc aatgtttcgg tcaaattatg
      241 agctcgtgca atgatttttc ctccacacac aatgactgct ccaattggga cctcattctt
      301 tttttcggct tcttttgctt caatgagtgc ctgtcgcata aaaacaatat cttgattttc
      361 ttttattttt agcattaaag aaattgaatt tacgagcgaa atttaattgt caaaattaaa
      421 atcattttta actaaaacaa ttattttgtg attttatttt attgtttcag aaaagttata
      481 actttgtttt ttaaaattaa ttttaagact ctttttaaga ataaaaaatt ttatgaaatc
      541 aaagttgtta acgctatttt gtttattttt ctctctcatc atttgggctg ttccaccagc
      601 aggttattat acttcatcta cattggatgg gaaaaatacg aacaatttgc gaattgcttt
      661 tcaggctatt attaccaatg gacatagtgt gacatcttat gatggtttat ggaatgctta
      721 caaaacaact gatataaatc cttcaacagg taaaatttgg gatatatatt ccaataattg
      781 taactttact tggggtaatg atcagtgtgg aaactacagt aaggaatgtg attgttacaa
      841 tcgtgaacat actactccac aaagttggtt taatggtgct tctcccatgt attctgattt
      901 attcaatgtt tatcctaccg acggttacgt aaacaataaa agaagcaatt atccttatgg
      961 agaagtcgga tctgctactt atacttctgg taatgggagc aaattgggta cttccaattt
     1021 ttcaggttat aatggaactg tttttgaacc gattgatgaa tataaaggcg atttagcccg
     1081 tacttatttt tacatggcta ctcgttatgc cgatcaatgc ggaacatgga caaatgggaa
     1141 tggttcagtc gtctatactt ctactcttgg acttactttt tatgctatca acttattttt
     1201 gaagtggagt cgtgaagatc ctgtcagcc
//
LOCUS       DAOTKS010000428         1185 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_97959_length_1185_cov_2.391150, whole genome shotgun sequence.
ACCESSION   DAOTKS010000428 DAOTKS010000000
VERSION     DAOTKS010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97959_length_1185_cov_2.391150"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="P5236_07070"
     CDS             <1..307
                     /locus_tag="P5236_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRU64160.1"
                     /translation="RNNIPDSRQEIVFFTTKTDGQLSPFSTIDYTKRKPFAETNVSIL
                     SLQTKPMHAPAEDDPPFPGDPGQLPIGDGIGFLLCLSLLYAFIRKSSYLFTHHKQEV"
     gene            complement(535..>1185)
                     /locus_tag="P5236_07075"
     CDS             complement(535..>1185)
                     /locus_tag="P5236_07075"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRU64161.1"
                     /translation="MGKIIGIALVGLTQFFIWMILTLAIVGIAGSLFNTNIDFNSLSQ
                     SNEIIKNEQIVNQAVSSSGAENGINYFLEMLQGIDFMQIIILFIVYFIGGYLLYASLF
                     AAVGSAVDTETDTQQFTLPLTLPIIFAIYAAIYSTQNPDGPLAFWCSLIPFTSPVVMM
                     VRLPFGVATWEILLSLVILILSFIGTTWLAGKIYRTGILMYGKKATWKELWKWMKY"
BASE COUNT          409 a          214 c          185 g          377 t
ORIGIN      
        1 acgaaacaat attcctgatt ctcggcagga gatcgtattt tttacaacta aaacagatgg
       61 tcaactttct cctttttcaa ctatcgatta cacgaaacgt aagccttttg cagagactaa
      121 tgtctctatt ttatctttac aaaccaagcc catgcatgct cctgcggaag atgatccacc
      181 ttttcccggt gatccgggac agttgcctat tggagatggt ataggatttc ttttgtgttt
      241 atccttactc tatgccttta ttagaaaatc gagttatttg ttcacccatc acaaacagga
      301 ggtataaaaa agtttatcta tattattatt aaatgctctt atttattgaa aatgaactat
      361 ttaaagaatc atttatcaac agattcaaca atttttttct ttttattttt attaaataaa
      421 caaagtaaga gagagaaaaa atcgtatttt ttcgggtaac ggacagatgc aaaaaaatga
      481 cttctgttta ttataacaat tcaggtattg ttgccatttt gggaaatacc ttagttaata
      541 tttcatccat ttccacaatt ctttccaggt agctttttta ccatacatta aaatgccagt
      601 acgataaatt tttcctgcta accacgtagt acctatgaat gaaagaatta aaatgaccag
      661 tgacagcaaa atttcccacg ttgctactcc gaaaggtaag cgaaccatca tcacaactgg
      721 agaagtaaaa ggaatcaagg aacaccagaa agccagtggt ccatcaggat tttgcgtact
      781 gtaaatggct gcataaatgg caaaaataat gggcaacgtg aggggtaaag taaattgttg
      841 tgtgtcggtt tcagtgtcaa ctgccgaacc tacggctgca aaaagtgaag catatagcaa
      901 ataacctcca ataaaataaa ctataaaaag aatgataatt tgcataaaat caataccttg
      961 taacatttca aggaaataat ttatcccatt ttcagctcct gaagaagaaa ctgcctgatt
     1021 cacaatctgt tcattcttta ttatttcatt cgattgtgaa agtgaattaa aatcaatatt
     1081 tgtattaaat aaacttccag caatgcctac aattgctaat gtcaagatca tccagataaa
     1141 aaattgtgtc agtcctacta aagcaatacc aatgattttt cccat
//
LOCUS       DAOTKS010000429         1182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_98308_length_1182_cov_4.241349, whole genome shotgun sequence.
ACCESSION   DAOTKS010000429 DAOTKS010000000
VERSION     DAOTKS010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98308_length_1182_cov_4.241349"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1182)
                     /locus_tag="P5236_07080"
     CDS             complement(<1..>1182)
                     /locus_tag="P5236_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HRU64162.1"
                     /translation="GLKYAAIICAGSVLVWFVIIPILPLFNDSFLHILNPVFTENLIN
                     VPPEIIFRDFARHIGIGGIAMAGIIGVINSWSIIKNAIRLASKEFKKNKPVETTVKRT
                     QRDLPMKIIIVGIVVTLLLTFAYFYFGVVGNLLQAIVGILVIGIITFLFTTVAANAIA
                     IVGTNPVSGMTLMTLILSSVILVAVGLKGPTGMIASLVMGGVVCTALSMAGGFITDLK
                     IGYWIGTTPAKQETWKFLGTLVSAATVGIVIMILNKTYGFTGEKALVAPQANAMAAVI
                     EPLMSGGGAPWLLYAFGALIALVLNFLKVPALAFALGMFIPFELNIPLLIGGGINWLV
                     STRTKDKKLNQVRQEKGTLLASGFIAGGALMGVVSAALRFGGLDFINNKWAESAAAEW
                     LA"
BASE COUNT          429 a          265 c          184 g          304 t
ORIGIN      
        1 gccaaccatt ccgctgcagc actttcagcc catttattat tgataaaatc caatccacca
       61 aaacgtaaag ctgcacttac cacacccatc aaagcaccgc cagctataaa tcctgaagcc
      121 agtaaagtac ctttttcctg acgaacctga ttaagttttt tatcttttgt tctagtagaa
      181 accagccagt taatcccacc accgattaac aatggaatgt tcaattcaaa tggaataaac
      241 attccgagtg caaaagccag tgcagggact ttcaaaaaat tcaagacaag agcaatgagt
      301 gctccaaaag catataaaag ccatggagct cctccacctg acatcaacgg ttcgatgaca
      361 gcagccattg cattagcctg cggggctacc agagcttttt caccagtaaa gccataagtt
      421 ttatttaaaa tcatgatcac aatgccaact gttgccgcag aaactaatgt gccaagaaat
      481 ttccacgttt cctgtttagc tggtgtggtc ccaatccaat aaccaatttt taaatcggta
      541 ataaatccac ctgccataga taatgctgta caaacaacgc ctcccataac aagtgaagct
      601 atcatgccgg tcggtccctt caaacctact gccacaagaa tcactgacga taaaattaaa
      661 gtcataaggg tcattccaga aacaggattt gttcctacaa tggcaatagc atttgcggct
      721 actgtagtaa acaaaaaagt aatgatacca atgactaaaa tgccaactat tgcctgcaat
      781 aaattgccta cgacaccaaa ataaaaataa gcaaaagtca acagtaaagt aacaacaatt
      841 ccaacaataa tgattttcat cggaagatca cgttgcgtgc gttttaccgt tgtttcaaca
      901 ggcttatttt ttttaaattc ttttgaagct aaacgaatcg catttttaat gatgctccat
      961 gaattgataa caccaatgat gcctgccatt gcaattccac caatgccaat atgacgagca
     1021 aaatcacgaa aaataatttc aggaggaaca ttaattaaat tttcagtaaa aacaggattc
     1081 aatatgtgta aaaaactatc attgaataaa ggaagaattg gaataataac aaaccatacc
     1141 agaactgaac ctgcacaaat aatagcagca tattttaaac ca
//
LOCUS       DAOTKS010000430         1141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_103843_length_1141_cov_5.109576, whole genome shotgun
            sequence.
ACCESSION   DAOTKS010000430 DAOTKS010000000
VERSION     DAOTKS010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103843_length_1141_cov_5.109576"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="P5236_07085"
     CDS             complement(<1..776)
                     /locus_tag="P5236_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HRU64163.1"
                     /translation="MTLRNELQHRILILDGAMGTMIQRQKLSEADFRGSRFIDWSKQL
                     KGNNDLLVLTYPSLIQSIHRKYLEAGADIIETNTFNAQRISMADYELGEYVREINLVA
                     AHLAREVANEFTAKNPEKPRFVAGSVGPTNKSASLSPDVNRPAFRSVTFDELVTAYKE
                     QMIALIEGGVDTLLIETVFDTLNAKAAIFAAQEAMKEANRHVELMVSATIADKSGRIL
                     SGQTVQAFLVSIAHADLLSVGLNCSFGAKDLKPYLQEVSK"
     gene            971..>1141
                     /locus_tag="P5236_07090"
     CDS             971..>1141
                     /locus_tag="P5236_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRU64164.1"
                     /translation="MKRLVLVRHGESIWNKENRFTGWTDVDLSEKGIEEAIKAGKILK
                     EKGFFFQKAYTSY"
BASE COUNT          399 a          203 c          189 g          350 t
ORIGIN      
        1 tatttgctaa cttcttgtaa ataaggtttt aaatcttttg ctccgaaaga acaatttaag
       61 ccaactgata acaaatcagc atgagcaata gaaaccagaa aagcctgaac agtttgtccg
      121 gataagattc ttccactttt atcagcaata gtagctgaaa ccattaattc aacatgtctg
      181 tttgcttctt tcatggcttc ctgagctgca aaaatagcag ctttggcatt caaagtatca
      241 aaaactgttt ctatcaacaa ggtatctact ccaccttcaa tgagtgcgat catttgttct
      301 ttgtaagctg tcaccaactc atcaaaagtc acagaacgaa aagctggacg atttacatca
      361 ggcgataatg aagcactttt atttgtaggt ccaaccgaac ccgcaacaaa tcgtggtttt
      421 tcaggatttt tggcagtaaa ctcatttgcc acttcacgcg ccagatgagc agcaaccaga
      481 ttgatttctc gaacatattc tcccagctca taatcagcca ttgaaattcg ttgagcattg
      541 aaagtatttg tttcaatgat atcagctcca gcttccagat attttctatg aatagattga
      601 attaatgatg gatacgttaa tacgagcaaa tcattgttcc ctttcaactg tttcgaccaa
      661 tctataaagc gagaaccacg aaaatctgct tcagaaagtt tctgacgttg tatcatagtg
      721 cccatcgcac catctaaaat caaaatgcga tgttgaagtt catttctaag agtcattgaa
      781 agatttttca aaagaagaat ttacgattat ttatgacaaa aataaagata atctgtcaat
      841 caacaaaaaa ctaatttaaa tggtcgactt ataaattcaa cttttaaacc tttgatttct
      901 ggtgaatttt agatatcttt gcatcaagaa aagaattatt cattaaatca atattttaaa
      961 aatctgcaaa atgaaaagat tagtattagt aagacatgga gaaagtatat ggaacaagga
     1021 aaatcgtttc acaggctgga ctgatgtaga tttatcagaa aagggcattg aagaagcaat
     1081 aaaagcgggt aagattttaa aagagaaagg gtttttcttc cagaaagctt atacttctta
     1141 c
//
LOCUS       DAOTKS010000431         1135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_104752_length_1135_cov_2.990741, whole genome shotgun
            sequence.
ACCESSION   DAOTKS010000431 DAOTKS010000000
VERSION     DAOTKS010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104752_length_1135_cov_2.990741"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..220)
                     /locus_tag="P5236_07095"
     CDS             complement(86..220)
                     /locus_tag="P5236_07095"
                     /inference="COORDINATES: protein motif:HMM:NF040909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG-related small transporter subunit"
                     /protein_id="HRU64165.1"
                     /translation="MINNVEAALEIGAIGIVGIFIFMLIFYAAIKLILKLFPEKEKTE
                     "
     gene            complement(233..>1135)
                     /locus_tag="P5236_07100"
     CDS             complement(233..>1135)
                     /locus_tag="P5236_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HRU64166.1"
                     /translation="ATEMFPLLIFIAIGAMIDFTPLFNNPHLLLFGAAAQFGIFMTMF
                     IATLFGFDLKEAASIGIIGAADGPTSIFVASRFAPHLLGPISVAAYSYMALVPIIQPP
                     VIRLLTSKKERLIRMQYSSKKVSRKALIAFPIVVTIISGLIAPASLSLIGFLMFGNLI
                     RECGVLNKLSQSAQNELASLVTILLGITIASTMTAERFVTLGTLTIIALGLLAFVFDT
                     FGGVIFAKLLNLVLPANKKMNPMIGACGISAFPMSARVIHGMGLKEDPHNFLLMPAVS
                     ANVGGQIGSVVAGGLILTLVNLFA"
BASE COUNT          404 a          220 c          212 g          299 t
ORIGIN      
        1 gtttgtttat tttttgagca aaaataaata ttattttttt atattagaaa gcaatttagg
       61 atgaggcaaa tagtgacctt ttttattatt cggttttttc cttttcaggg aaaagtttca
      121 aaataagttt aatggcagca taaaatataa gcataaaaat gaatatgcct acaataccga
      181 ttgctccgat ttcgagagca gcttcaacat tattgatcat aaatttaata ttttaagcga
      241 ataaatttac caaagtaaga ataagtccac cagccaccac agatccaatc tggcctccaa
      301 catttgcact gacggcaggc attagcaaaa agttatgtgg atcttcttta agacccatac
      361 catgaattac ccttgcagac ataggaaaag cggaaattcc gcaggctcca atcattggat
      421 tcattttctt gttggcaggc aaaaccaggt tcaaaagctt agcgaaaatg actccaccaa
      481 aagtgtcgaa aacaaaagcc aacaatccca atgcaataat ggtaagtgtt cccagtgtta
      541 cgaaacgttc agctgtcatg gtgctggcaa tggttatacc caataaaata gttaccaaac
      601 ttgccagttc attctgagct gattgcgaca acttgttaag tacgccacac tcgcgaatca
      661 aatttccaaa cattaaaaat ccgatcagtg aaagagaagc gggagctatc aatcctgaaa
      721 tgattgttac gacaataggg aaagcaatca atgccttacg tgaaactttt ttggaactgt
      781 attgcatccg aatcagtctt tcttttttcg aagtcaacag tcgaataacc ggaggctgaa
      841 taatgggaac caatgccata taggaatatg ccgcgacgga tataggtccc aaaagatgcg
      901 gtgcaaaacg cgaagcgaca aaaatggaag ttggtccgtc agcagctcca ataattccaa
      961 tggaagcagc ttctttcaaa tcgaatccaa acaaagtggc aatgaacata gtcataaaaa
     1021 taccaaattg tgcagccgct ccgaaaagta gcaaatgagg attgttgaac aagggcgtaa
     1081 aatcaatcat agccccaatt gcaataaaaa tcaataaagg aaacatttca gtagc
//
LOCUS       DAOTKS010000432         1120 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_107020_length_1120_cov_11.851643, whole genome shotgun
            sequence.
ACCESSION   DAOTKS010000432 DAOTKS010000000
VERSION     DAOTKS010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107020_length_1120_cov_11.851643"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..1051)
                     /locus_tag="P5236_07105"
     CDS             complement(59..1051)
                     /locus_tag="P5236_07105"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HRU64167.1"
                     /translation="MEKTNTENIELYCVRAEFGTYTKQFIDGGYVAIGWLPNNDLSAI
                     ASRDELYTLYKKEYPKDTSNVVIGQQVGQIARFLLEIKPGDYVITPALDTEFIYYGIV
                     EENPYYFSDGADGCPYRHRKKVEWHKEPIQRSQFSVPFQNTIRSSLTVFCISHKKNFF
                     TTIGKPELVPESEKKVEFDYYTSVLDRILELDDQEFEILITHILNALGFEGSEHKGKV
                     GDGGVDATGELNVENMAKIKLFVQAKRYKLGSKINANVVKALRANIPAGGQGAFITTA
                     DYQEAAKKIAVEQGFPRIGLINGEQLVDILAEHWNDIPVEFRDKLGLKIGLVPN"
BASE COUNT          317 a          252 c          181 g          370 t
ORIGIN      
        1 taaattgaaa gaactgccca gcgcccatat tgctctatta cttttttaag ctttttcatc
       61 aattagggac caatccgatt tttaaaccta atttatctct gaactccaca ggtatatcat
      121 tccaatgctc ggccaaaata tctaccaact gttcgccatt gattaatcca atgcgtggaa
      181 acccctgctc aacagcaatt tttttggctg cttcttggta atcggcagta gtaataaacg
      241 caccttgtcc gcctgcagga atgttagccc ttagtgcttt tacaacattt gcatttattt
      301 ttgaaccgag cttgtatcgt ttggcttgta caaataattt gattttagcc atattttcta
      361 catttaactc acctgtagca tccacgccac catcacctac tttaccttta tgttccgatc
      421 cttcaaaacc aagagcattt aaaatatgtg tgatcagaat ttcgaattct tggtcgtcaa
      481 gttccaaaat tctatccagt acagaagtgt aataatcaaa ttccactttc ttttcacttt
      541 ccggtaccag ttcaggcttg ccaatggttg tgaaaaagtt ctttttatgg ctgatgcaaa
      601 aaacggtgag cgacgaacgg atagtattct gaaaaggaac ggaaaactga ctccgttgaa
      661 tgggctcttt gtgccattcc acttttttcc ggtgccgata aggacaacca tctgctccat
      721 ctgaaaaata atagggattt tcctccacaa tgccgtaata aataaattct gtgtccagtg
      781 ccggagtaat tacataatcc cctggtttta tttctaaaag aaaacgggca atttgtccca
      841 cctgctgacc aatcactaca ttgctggtgt ctttcggata ttctttcttg tatagcgtgt
      901 atagctcatc acgactggct atcgcagaca aatcattatt cggcaaccaa ccaatagcta
      961 cgtagccacc atcaataaat tgtttagtat aagtgccaaa ttctgctctt acacagtata
     1021 gttcaatatt ttcagtattc gttttttcca tagtcttaaa atttaacagt tatctctcca
     1081 ctcattaact ttggcaacaa catgtctcgt aatgatgtga
//
LOCUS       DAOTKS010000433         1113 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_108097_length_1113_cov_6.517958, whole genome shotgun
            sequence.
ACCESSION   DAOTKS010000433 DAOTKS010000000
VERSION     DAOTKS010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108097_length_1113_cov_6.517958"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="P5236_07110"
     CDS             complement(<1..470)
                     /locus_tag="P5236_07110"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRU64168.1"
                     /translation="MTILVTGANGLLGHWVVQELLDRNYTVKIVVRTWKNIFFDLKSV
                     EVITGNFTNYKTLKKAATGCDAIVHLAAITATNLLHYEDYQKINVEGSAQVIRVANEL
                     QIKTLIYISSSNTIGYGSKDQPANEESPFQFPFTESFYAQSKKEAEELFSEFAL"
     gene            complement(656..1111)
                     /locus_tag="P5236_07115"
     CDS             complement(656..1111)
                     /locus_tag="P5236_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HRU64169.1"
                     /translation="MEKIDELDRKILKIITQNARLPFRDVAAECGVSRAAVHQRVQRL
                     IDMKVITGSGYLVDPKKLGYQMCVYIGITLERGSMYKKVIQELEKIPEIVESHYTLGP
                     YTILIKLYAKNDEHLMELLNGKIQEIKGVAATETLTSLDQRICRTIPIE"
BASE COUNT          370 a          185 c          172 g          386 t
ORIGIN      
        1 tgtaaagcaa actcagaaaa aagctcttct gcttcttttt tgctttgtgc ataaaaagat
       61 tcagtaaaag ggaattgaaa aggagattct tcattggcag gttgatcttt acttccatag
      121 ccaatggtat tagaagagct gatgtatatt aatgttttga tttgtagttc gttggcaact
      181 cgaattacct gagctgaacc ttctacatta attttttgat aatcttcgta atgaagcaaa
      241 tttgtggcgg taatagcagc aagatgaaca atggcatcac atcctgtagc agcctttttc
      301 aatgttttat aattagtaaa attgccagta ataacttcta ctgattttaa atcaaaaaaa
      361 atatttttcc aagttcgaac tacgatttta acggtataat tgcgatctaa taattcttga
      421 acgacccaat gtcccaataa tccatttgca cctgttacta aaattgtcat acaaatcaat
      481 ttataaaaaa attgatcaac aaataaataa aactcatcct gataaattta tcagaatgaa
      541 aggagaaaac tattcaataa atagaattta aattttgttt aaatgatatt ttaaagggat
      601 ttaaaaaatc gtcaactagt ttttaaggga tttttgattt tccacttaag gaattttatt
      661 caattggaat tgttcggcag attctttggt cgagcgaagt aagtgtttca gtagcggcaa
      721 ctccttttat ttcttgaatt tttccattca gtaattccat tagatgttca tcgttttttg
      781 catacaattt aatcaaaatg gtataaggtc ccaaagtata atgcgattca acgatttctg
      841 gaattttttc caattcctga atgacttttt tatacataga gccacgttca agcgtaatgc
      901 caatataaac acacatttga taccccagtt ttttagggtc cactaaataa ccagatcctg
      961 taatgacttt catatcgatc agtcgttgaa cacgctgatg tacagcagcc cgggaaactc
     1021 cacattcagc agctacatcc ctaaatggca aacgagcatt ttgggtaata atttttaaaa
     1081 tttttcgatc cagttcatca attttttcca tag
//
LOCUS       DAOTKS010000434         1112 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_108243_length_1112_cov_3.225166, whole genome shotgun
            sequence.
ACCESSION   DAOTKS010000434 DAOTKS010000000
VERSION     DAOTKS010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108243_length_1112_cov_3.225166"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1112)
                     /gene="rpoB"
                     /locus_tag="P5236_07120"
     CDS             complement(<1..>1112)
                     /gene="rpoB"
                     /locus_tag="P5236_07120"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212060.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRU64170.1"
                     /translation="LNENGKFIRNKVKARLGADFPIVDPSDVDLMDVSPSQIASIAAA
                     LIPSLEHDDANRALMGSNMMRQAVPLLRCEAPVVATGIEGRVIRDSRTQILAEGEGVV
                     EYVDATSIKIRYDRTDEEELVSFDSNIKEYKLSKFLKTNQNTTIDLRPLVRKGDKVHA
                     GQIITEGYCSEKGELALGRNLKVAFMPWKGNNFEDAIVISERVVREDIFTSVHVVEQL
                     LEVRETKRGPEEFTADIPNVSEEATKNLDENGIIRIGAWVEPGDIIIGKITPKGESDP
                     SPEEKLLRAIFGDKAGDVKDASLKATPSLSGVVIDKRLFSKALKNKKSKLAEKAILPK
                     VEEEFEAQTKALKEALIEKLMIILNNKTSAGVKDFLG"
BASE COUNT          313 a          246 c          167 g          386 t
ORIGIN      
        1 cctaaaaaat ctttaacacc cgctgaagtt ttattgttta gaataatcat caatttttca
       61 attaaagcct cttttaatgc ttttgtttga gcttcaaatt cctcttccac tttgggcaga
      121 atagcctttt cagcaagttt tgatttctta tttttcaaag cttttgaaaa taaacgtttg
      181 tctattacga caccagataa ggaaggagtt gcttttaatg aagcatcttt tacatcgcca
      241 gctttatcgc caaaaatagc acgaagtaat ttttcttctg gtgatggatc tgattctcct
      301 tttggagtaa tctttccaat aataatatcg cctggctcta cccatgcacc aatacggata
      361 attccatttt catccaaatt ttttgttgct tcttcactaa catttggaat atctgctgta
      421 aactcttctg gtccacgttt tgtttctctt acctctaata actgttctac cacatgtacc
      481 gaagtaaaaa tatcttcgcg taccactctt tcgctaatca caatagcatc ttcaaagtta
      541 tttcctttcc agggcataaa cgccaccttc aaatttctgc ctaatgctaa ttctcctttt
      601 tcactacaat atccttctgt aatgatttga cctgcatgaa ctttatcacc tttccgaacc
      661 aatggtcgta aatcaatggt agtattttga ttagttttta aaaatttaga taatttgtat
      721 tcttttatgt ttgaatcaaa acttacgagc tcttcttcgt cggttcgatc ataacgaatc
      781 ttgatagaag tggcatcaac atattctacc acaccttccc cttcagccag tatttgagtt
      841 cttgaatccc gtataacccg tccttcgatg cctgttgcta caacaggagc ttcgcatcgt
      901 aacaagggga cagcctgacg catcatatta gaacccatca aagctcgatt ggcatcatca
      961 tgctccagag atggaatcaa ggctgctgca atagaagcaa tttgcgaagg agacacatcc
     1021 attaaatcta catcagaagg atcaactatg gggaaatcgg ctcctaaacg ggcttttact
     1081 ttattgcgaa taaattttcc gttttcattc aa
//
LOCUS       DAOTKS010000435         1108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_108845_length_1108_cov_3.124406, whole genome shotgun
            sequence.
ACCESSION   DAOTKS010000435 DAOTKS010000000
VERSION     DAOTKS010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108845_length_1108_cov_3.124406"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..>1108
                     /gene="queA"
                     /locus_tag="P5236_07125"
     CDS             142..>1108
                     /gene="queA"
                     /locus_tag="P5236_07125"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HRU64171.1"
                     /translation="MKLSQFKFKLPDELIAQYPAPHRDESRLMVVHRKTGEIEHKIFK
                     DVLDYFNENDLFVLNDTKVFPARLYGNKEKTGAQIEVFLLRELNPDLKLWDVLVEPAR
                     KIRIGNKLYFGNDDSLVAEVVDNTTSRGRTLRFLFDGTHEEFKKTLYSLGETPLPRYI
                     NRSVEKEDAERFQTIFAKNEGAVAVPSAGLHFSRELMKRMEIKGIEIAFLTSYMNLGN
                     FREIDVEDLTKHKMDSEQISVPEETAEKVNKAQENRQNICAVGATVMRALESVACTDG
                     KIKSFEGWTNKFIFPPYDFTVANALITNFHFPCSTFLMLTAAFGGY"
BASE COUNT          370 a          171 c          200 g          367 t
ORIGIN      
        1 atcttttttt aattaaaatt agcaataata ttctttataa ttagctgttt tgattgattt
       61 tttttctttt ctctttttaa aaaataaaaa ggtattaata agaaagtgtg tttagtttaa
      121 caatatcaat gttttagttt tatgaaatta tctcaattta aatttaagtt acccgacgag
      181 ttgattgccc aatatccagc tccgcatcgt gatgaatcac gattaatggt agtgcatcga
      241 aaaacaggcg aaattgagca taaaatcttt aaagacgtgc ttgattattt taatgaaaat
      301 gatctttttg tattgaacga tacaaaagtg ttcccagctc gattgtatgg aaataaggaa
      361 aaaacaggag ctcaaattga agtattttta ttaagggaat taaaccctga tttaaagctg
      421 tgggacgtcc ttgtagagcc tgctcgtaag atacgaattg gtaacaagct atattttggc
      481 aacgatgatt cacttgtagc tgaagtagtt gacaatacta cttccagagg aagaactctt
      541 cgtttcttat ttgatggcac tcacgaagaa tttaaaaaaa cactttattc attaggagaa
      601 acaccactgc cacgctacat taatcgctcc gttgaaaaag aagatgccga acgttttcaa
      661 actatttttg caaaaaatga aggtgctgta gctgttcctt cagcaggttt gcattttagc
      721 cgagaattaa tgaaacgaat ggaaattaaa ggcattgaaa tagctttttt aacttcctat
      781 atgaatttag gaaattttcg tgaaatagat gtagaggatc tcaccaagca taaaatggat
      841 tcagaacaaa tttctgttcc agaagaaacg gctgaaaaag taaataaagc tcaggaaaac
      901 agacaaaata tttgtgccgt tggtgctacc gttatgcggg cattagaatc cgtagcatgc
      961 actgatggca aaataaaatc ttttgaagga tggacaaata aatttatttt ccctccttac
     1021 gattttactg tggctaatgc tttgataacc aattttcatt ttccctgctc aactttcttg
     1081 atgttaaccg ccgcttttgg tggatatg
//
LOCUS       DAOTKS010000436         1097 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_110577_length_1097_cov_3.805182, whole genome shotgun
            sequence.
ACCESSION   DAOTKS010000436 DAOTKS010000000
VERSION     DAOTKS010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110577_length_1097_cov_3.805182"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1097
                     /locus_tag="P5236_07130"
     CDS             <1..>1097
                     /locus_tag="P5236_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HRU64172.1"
                     /translation="QPKREMRAVWIATVANIDWPSSNRLTPREQRIEITNMLDKLAQN
                     NINAIILQVRPMADAFYPSSIDPWSHWLTGKQGFAPNPFYDPLQFIITEAHKRCMEVH
                     AWINPYRVTMNDNLSVLSPNHLFYQKPELFVRYGGKYYFNPGLDETRQILNNVIKEIV
                     EHYDIDGIHFDDYFYPYPVKGEEFPDEDTFRKNPRGFAPDQKADWRRNNVNMVIAEVQ
                     QTIKNLKPWVEFGVSPFGVWRNNNVDPRGSATRAGIQNYDDLYADILKWLKEGNIDYV
                     VPQLYFEIGKKVADYEVLLKWWSENSYGKNLYPGLIPSSLGSNKNSAWSKGNEIIRQL
                     RLNQQYPQVEGAVFFSAKTFLQNRLGLNDSL"
BASE COUNT          349 a          183 c          217 g          348 t
ORIGIN      
        1 cccagccaaa gcgtgaaatg cgagcagtct ggattgccac agtagccaat attgattggc
       61 ctagttctaa ccgacttaca ccccgtgaac aacgaataga aataaccaat atgctggaca
      121 aacttgctca aaacaatatc aatgctatta ttcttcaggt tcggcctatg gcggatgctt
      181 tttatccttc ttccattgat ccgtggtcgc attggttgac aggcaaacaa ggctttgctc
      241 caaatccttt ctatgatcct ttacaattta ttattacaga agcacataaa cgttgcatgg
      301 aagttcatgc ctggatcaat ccttatcgtg ttacaatgaa cgataatttg tcagtattgt
      361 cgcctaatca tttgttttat caaaaaccgg aattgtttgt tcgttacggc ggcaaatatt
      421 attttaatcc aggtctggac gaaacccgac agattttaaa taacgtcata aaagaaattg
      481 ttgaacatta tgatattgat ggaattcatt ttgatgacta tttttaccct tatccagtaa
      541 aaggagaaga atttcctgat gaagatactt tcagaaaaaa tcctcgtggt tttgctcctg
      601 atcaaaaagc tgattggaga aggaacaatg tgaatatggt cattgctgaa gttcagcaaa
      661 ctattaaaaa tttaaaacca tgggtagaat ttggtgtaag tccttttggt gtttggcgaa
      721 acaataatgt tgatcctcga ggttctgcca cacgtgctgg cattcaaaat tatgatgatc
      781 tttatgcaga cattttgaaa tggttaaaag aaggtaatat agattatgta gttcctcagt
      841 tgtattttga aattggcaag aaagtagctg attatgaagt gcttttaaaa tggtggagcg
      901 aaaattctta tggtaaaaat ttatatccag gtttgattcc gtcttcatta gggagcaata
      961 aaaattcggc atggtcgaaa ggaaatgaaa ttattcgtca actaaggctc aatcaacaat
     1021 atcctcaagt ggaaggagct gtgtttttta gtgccaaaac ttttttacaa aacaggctag
     1081 gattgaatga tagttta
//
LOCUS       DAOTKS010000437         1091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_111509_length_1091_cov_3.090734, whole genome shotgun
            sequence.
ACCESSION   DAOTKS010000437 DAOTKS010000000
VERSION     DAOTKS010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111509_length_1091_cov_3.090734"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1091)
                     /locus_tag="P5236_07135"
     CDS             complement(<1..>1091)
                     /locus_tag="P5236_07135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRU64173.1"
                     /translation="TAIVCVTNDLTTDQRVHKTCLTLQKCDYQTIEVGRLLPESLPID
                     RPYSVRRFKLWFRKGPQFYAEYNIRLFFYLLFSKVDLIFANDLDTLPAAFLAAKIRKK
                     RLIYDTHEYFTETPELVNRPRTKKIWEKIENFIFPKLTDIITVNQSIANLYESKYHKK
                     IYVCRNIPLDFTPTRLKTREELGLPLDKHILILQGTGINVQRGAEEAVLTMQYLEDCV
                     LLIIGSGDIIPELQKMIDQYQLQEKIIIKPKMLFQELRQYTMNSDLGLAIDKDTNLNY
                     HYSLPNKLFDYIHAEIPILSSGLPEIKQIIDQYDIGYYIQNHHPKHIAEVIQNIFNNP
                     QRYALVKQNTAKAKAELNWEKEEQILIEL"
BASE COUNT          346 a          155 c          185 g          405 t
ORIGIN      
        1 ataagttcta tcaatatttg ttcttctttt tcccaattta attctgcttt tgctttggca
       61 gtattttgtt taacaagagc ataacgttgt gggttgttaa aaatattttg aattacttca
      121 gcaatatgtt ttggatggtg attttgaata tagtagccaa tatcatattg atcaataatt
      181 tgtttaattt ctggtaaacc tgatgataaa attggaattt cagcatgaat ataatcaaaa
      241 agtttattgg gaagcgaata atggtaatta agattggtgt ctttatcaat agccagccct
      301 aaatcgctat tcatagtata ttgtctgagt tcttgaaaaa gcatttttgg ttttatgatt
      361 attttttcct gaagttgata ttgatcaatc atcttttgaa gttcgggaat gatatctcca
      421 cttccaataa ttaacaacac acaatcttca agatattgca tagttaaaac agcttcttcc
      481 gcaccacgct gcacattaat tcctgttcct tgcaaaataa gaatgtgctt gtctaagggc
      541 aatcccaatt cttcacgagt ttttaatcga gtaggagtga agtcaagtgg gatatttcta
      601 caaacataaa tttttttatg atattttgat tcatatagat ttgcaataga ttgatttaca
      661 gtgattatat cagttaattt aggaaaaatg aaattttcaa ttttttccca gatttttttt
      721 gtgcgaggtc ggtttactaa ttcaggagtt tctgtaaaat attcatgggt gtcgtaaatt
      781 aatctttttt tgcgaatttt tgctgctaaa aatgctgctg gtagtgtatc caaatcgttg
      841 gcaaaaatca gatcaacttt tgaaaagagt aaataaaaaa acaagcgaat gttatattcc
      901 gcataaaatt gaggaccttt acggaaccag agtttaaatc tacgaacaga ataaggtcta
      961 tcgattggta aactttcagg caacaatcgt cctacttcaa tggtttgata atcacatttt
     1021 tgtaaagtta agcacgtttt atgaacacgt tgatcggtag ttaaatcatt agttacgcaa
     1081 actatggctg t
//
LOCUS       DAOTKS010000438         1079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_113572_length_1079_cov_3.004883, whole genome shotgun
            sequence.
ACCESSION   DAOTKS010000438 DAOTKS010000000
VERSION     DAOTKS010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113572_length_1079_cov_3.004883"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            591..>1079
                     /locus_tag="P5236_07140"
     CDS             591..>1079
                     /locus_tag="P5236_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HRU64174.1"
                     /translation="MNVEKLLSSLEAKHPGEKEYLQAVHEILTSINEIYSQHPEFEKA
                     KIVERLVEPDRIITFKVPWIDDEGNVQINTGYRVQFNNAIGPYKGGLRFHPSVNLSIL
                     KSLAFEQIFKNALTTLPMGGAKGGSDFNPKGKSEMEMMRFCQSFMTELWRYLGPEIDV
                     PAG"
BASE COUNT          380 a          178 c          156 g          365 t
ORIGIN      
        1 agagcaacag ccttctaagc tgtgggtcgt gggttcgaat cccgccgaga tcaccttcca
       61 tcaataagaa atttcttctc ctgaaagcca tttgtgaggt ttaattgtca ttcctacttt
      121 tcttattcat cctttttaca tttgacaaca acggctttaa caacaatgta tatggcaagt
      181 gtatttaata atagaaaatt gccagattaa taatcgatta ttatctttta taaaactaaa
      241 cagtaagaaa catttcaaaa aagctaaatt ttgaaaaacc gtatactatg gttcactctc
      301 ccaacaatca ttttttgaac tccctttcat ccaaatcaat taaaacatat ttcttctaaa
      361 aatcatttat tagtagataa ttacttcctt ttttaaaatt ttattattca aatgataaaa
      421 atttctatca aatgtttgca atatgaaata aaaagtctaa atttgcactc gaaaaaaatt
      481 acctcacatc ttatgcgtga tttgaactta ttttcattaa ctaagtatat tattgtttat
      541 ttaaatattg agcataaaca taaattacta acttctaaaa aataaacatt atgaatgttg
      601 aaaaattatt atcatcactt gaagccaaac atccaggaga aaaagaatat ttacaagctg
      661 ttcatgaaat attaaccagt attaatgaaa tttattccca gcatcctgaa tttgaaaaag
      721 caaaaattgt tgaacgtttg gtagaaccag accgaattat tactttcaaa gttccctgga
      781 tagatgacga gggaaatgtg cagataaata ctggttatcg cgtgcaattc aacaatgcta
      841 ttggacctta taaaggcggt ttgcggtttc atccttcagt aaacctatca attctgaaat
      901 cgcttgcttt tgagcaaatt tttaaaaatg ctttaacaac tttaccaatg ggtggagcta
      961 aaggaggttc tgattttaat cctaaaggaa aatctgaaat ggaaatgatg cgattctgtc
     1021 aatcttttat gaccgaactt tggcgttatc ttggacctga gattgatgtg ccagcaggt
//
LOCUS       DAOTKS010000439         1065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_115898_length_1065_cov_4.090099, whole genome shotgun
            sequence.
ACCESSION   DAOTKS010000439 DAOTKS010000000
VERSION     DAOTKS010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115898_length_1065_cov_4.090099"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1065
                     /locus_tag="P5236_07145"
     CDS             <1..>1065
                     /locus_tag="P5236_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRU64175.1"
                     /translation="LSYTRNIGIMAHIDAGKTTTSERILYYTGLTHKMGEVHDGTATM
                     DWMEQEQERGITITSAATTTYWNYGGQKYKINLIDTPGHVDFTVEVERSLRVLDGAIA
                     TFCAVGGVEPQSETVWRQADKYKVPRICFVNKMDRSGADFFGVIRQIKEVLGANPCPI
                     QIPIGVEEKFKGVVDLIKMKAIFWRDETLGAEYTEEEIPADLINEAKEWREKLLEKVV
                     EFDDALMQKYFDDPLTITEKEINAVIRKGTLSREIYPVICGSAFKNKGIQTMLDAVCA
                     YLPSPLDNPEIVGKDPRFLDKTVIRHPDPNEPLCALAFKIATDPYVGRLCYFRVYSGK
                     IESGSYVYNPRTNKNERISR"
BASE COUNT          343 a          161 c          237 g          324 t
ORIGIN      
        1 atttaagcta tacaagaaat attggcatca tggcacacat cgatgccggg aaaactacta
       61 cttctgaacg tattttgtac tatacaggct tgactcacaa aatgggtgaa gtgcatgatg
      121 gtacagcaac aatggattgg atggaacagg agcaggaacg tggtattact attacttctg
      181 ctgctactac aacttattgg aattatggag gtcaaaaata taaaatcaat ttgattgaca
      241 ccccggggca tgttgatttt acggttgaag ttgaacggtc tttgcgagta cttgatggtg
      301 ctatagctac tttttgtgca gtagggggtg tagaacctca atcagaaact gtatggcgtc
      361 aagcagataa atataaagtt cctcgtattt gttttgtaaa taaaatggat cggtcaggag
      421 ctgatttttt tggcgtgatt cgacaaatta aagaagtact gggagctaat ccttgtccta
      481 ttcaaatccc catcggtgtt gaagaaaagt ttaaaggggt ggtcgacttg atcaaaatga
      541 aagctatttt ctggcgtgat gaaacgttag gagcagaata tacggaagaa gagattcctg
      601 ctgatttaat caatgaagcc aaagagtgga gagaaaagct gctggaaaaa gtagttgaat
      661 ttgatgatgc tttaatgcag aaatatttcg atgatcccct tacaattact gaaaaagaaa
      721 taaatgctgt tattcgtaaa ggtacacttt caagagaaat ttatccagta atttgtggtt
      781 ctgcttttaa aaacaaaggt attcaaacta tgcttgatgc agtatgtgcg tatttgccaa
      841 gtccgttgga taatccagaa attgtaggga aagatccacg ttttttagat aaaacagtga
      901 ttcgtcaccc tgatccaaat gaacctttat gtgcattggc atttaaaatt gcaacggatc
      961 cttatgtagg gcgtttgtgt tattttcgag tttattcagg aaaaattgaa tcaggttcat
     1021 atgtgtataa tcccagaact aataaaaatg aacgtatttc ccgga
//
LOCUS       DAOTKS010000440         1053 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_117967_length_1053_cov_3.120240, whole genome shotgun
            sequence.
ACCESSION   DAOTKS010000440 DAOTKS010000000
VERSION     DAOTKS010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117967_length_1053_cov_3.120240"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1053)
                     /locus_tag="P5236_07150"
     CDS             complement(<1..>1053)
                     /locus_tag="P5236_07150"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRU64176.1"
                     /translation="LVIDRTHQYEKALKNNDSYIFEFVDKPLNEIQKNTNKNKELAGV
                     LVITGDLSENSNAVTLYSEKQINLETKNYIAELLNDYVKEQKLRNYNVPDLEKIIENT
                     NQKIDIATVRWNEKGEEKLSSTELTIAIGIFSAMMVYIFILTYGSQVMSGVIQEKTNR
                     IVEIIISSVKPFQLMMGKIIGIALVGLTQFFIWMILTLVIVGIAGSLFNTNIDFNSLT
                     QSNEIIKNEQIVNQAVSSSEAENAMNHFLEMLQGVDFMQIITLFIVYFIGGYLLYASL
                     FAAVGSAVDSETDTQQFTLPLTLPIIFAIYAAIYSTQNPDGPLAFWCSLIPFTSPVVM
                     MVRLPFGVATWEILLS"
BASE COUNT          355 a          189 c          161 g          348 t
ORIGIN      
        1 gtgacagcaa aatttcccac gttgctactc cgaaaggtaa gcgaaccatc atcacgactg
       61 gcgaagtaaa aggaatcagc gaacaccaga aagccaacgg tccatcagga ttttgtgtac
      121 tgtaaatagc tgcataaatg gcaaaaataa tgggcaatgt gagtggcaaa gtaaattgtt
      181 gtgtgtcggt ttcactgtca actgccgaac ctactgctgc aaaaagggaa gcatatagca
      241 aatagcctcc aataaaataa acaataaaaa gagtgatgat ttgcataaaa tcaacacctt
      301 gcaacatttc aaggaaatga ttcatcgcat tttcagcttc tgatgaagaa actgcctgat
      361 ttacaatctg ttcattcttt attatttcat ttgattgcgt aagtgaattg aaatcaatat
      421 ttgtattaaa taaacttcca gcaataccta caattacaag tgtcaaaatc atccagataa
      481 aaaattgtgt cagtcctacc aatgcaatgc caatgatttt acccatcata agctgaaaag
      541 gtttcaccga cgaaataata atttctacaa tgcgattggt tttttcctga ataactccac
      601 tcattacctg tgaaccataa gtcagaataa aaatgtaaac catcatggct gaaaagatgc
      661 caatggcaat agtcaattct gtggaactca atttttcttc gcctttttca ttccagcgga
      721 cagtagcaat atcaattttt tgattggtat tttcaataat tttctctaaa tcaggaacat
      781 tataatttct taatttctgc tcttttacat aatcatttaa taactcagca atatagtttt
      841 ttgtttctaa attgatttgt ttttcagaat aaagcgttac tgcatttgaa ttttcggaca
      901 aatcacctgt gataaccaat acacctgcta attctttatt tttatttgtg tttttttgaa
      961 tttcgttcaa aggtttatct acaaactcaa aaatatatga atcattattt tttaaggctt
     1021 tttcatattg atgggttctg tcaatcacca aga
//
LOCUS       DAOTKS010000441         1039 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_120551_length_1039_cov_4.742886, whole genome shotgun
            sequence.
ACCESSION   DAOTKS010000441 DAOTKS010000000
VERSION     DAOTKS010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120551_length_1039_cov_4.742886"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..>1039
                     /gene="rsxC"
                     /locus_tag="P5236_07155"
     CDS             3..>1039
                     /gene="rsxC"
                     /locus_tag="P5236_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HRU64177.1"
                     /translation="MEEYKLSAGERTKVITLPQQVVVPLRHYAGKPSIPQVKRGDFLK
                     VGQLVGLADGFVSANVHSPVSGKVAKIDNALDAYGYKYPSVFIDVIGDDWLETIDLTP
                     EVDHTCELTSSEIIKKIAESGIVGLGGATFPTHVKLTIPAGKKAEVLIINGAECEPFL
                     TCDHQLMLEKGEEIIVGVTILMKALNVKKALIGIECNKNDAIINMKKWAKTEEGIEVV
                     PLQTKYPQGCEKQLIEALTGRRVPRGGLPVDVGAVVQNVATTFAVYEAVQKNKPLIER
                     IITVTGKSMSKPGNFWVRIGTPLSHVVEQVGGIPEDTGKIVAGGPMMGRALFSLEVAV
                     AKGTSGLLFIPS"
BASE COUNT          343 a          168 c          232 g          296 t
ORIGIN      
        1 atatagaaga gtataaatta tcagcagggg aaagaaccaa agttattaca ttacctcaac
       61 aagtggtagt gcctttacga cattatgcag gcaagcccag tattccacaa gtaaaacgag
      121 gcgatttttt aaaagtagga caattagtag gtttagctga tggctttgtg tctgcaaatg
      181 tgcattcgcc ggtttcaggt aaagttgcga aaattgataa tgctttagat gcgtatggct
      241 acaaatatcc atccgtattt attgatgtaa ttggcgatga ttggcttgaa acgattgatt
      301 taacccctga agtagatcat acatgtgaat tgacttcttc tgaaatcata aaaaaaattg
      361 cggaatctgg aattgttgga ttgggtggtg ccacttttcc tacccatgta aaacttacaa
      421 tacctgctgg gaaaaaagcg gaagtgctta ttattaatgg cgcagaatgt gaacctttcc
      481 ttacttgcga tcatcagtta atgcttgaaa aaggtgaaga aatcattgtt ggtgtcacca
      541 ttttaatgaa agctcttaat gttaaaaaag ccctcattgg aattgaatgt aataaaaatg
      601 atgcaatcat taatatgaaa aaatgggcaa aaacagaaga aggaattgaa gtagttcccc
      661 tacaaacaaa atatcctcaa ggttgtgaga aacagttaat tgaagccttg acaggacgtc
      721 gagttcccag aggaggtctt cctgtggatg ttggagcagt agttcaaaat gtagctacaa
      781 cttttgctgt ctatgaagcc gttcagaaaa acaaaccatt gatagagaga ataattacag
      841 ttacaggaaa atcgatgtcg aaaccaggaa atttttgggt acgaatcgga actccattaa
      901 gtcacgtagt tgagcaagtt ggcggtattc cagaagatac aggaaaaatt gtagcaggtg
      961 gaccgatgat gggacgtgct ttattttcgc tggaagtagc tgttgctaaa ggcacatcag
     1021 gcttgctttt catcccttc
//
LOCUS       DAOTKS010000442         1026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_123035_length_1026_cov_3.871267, whole genome shotgun
            sequence.
ACCESSION   DAOTKS010000442 DAOTKS010000000
VERSION     DAOTKS010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123035_length_1026_cov_3.871267"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..127)
                     /locus_tag="P5236_07160"
     CDS             complement(<1..127)
                     /locus_tag="P5236_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HRU64178.1"
                     /translation="MITVRLKPKKEESVKRFHPWIFSGAIQSIEGNPEEGDMVKVF"
     gene            complement(155..799)
                     /locus_tag="P5236_07165"
     CDS             complement(155..799)
                     /locus_tag="P5236_07165"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRU64179.1"
                     /translation="MKYSPKISKEEINKLPVATFTGKITVVDESSKVESSIAELRKND
                     IVGIDTETKPSFIKGVSHKVSLLQIATPEQCFLFRLNRIGFPKALADFLADKNITKVG
                     LSLHDDLRKLKKRHHFKPENFIDIQSIVKNYGIMELSLQKIYAIIFGEKILKSQKLSN
                     WEKPQLTEKQLQYAAIDAWATLQIYLQLMKEEPLAKKEIEKLVLENSPAKNGSN"
     gene            complement(806..>1026)
                     /locus_tag="P5236_07170"
     CDS             complement(806..>1026)
                     /locus_tag="P5236_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HRU64180.1"
                     /translation="YQLADVDIMNDALVTCLVTFAEHWGQKLLNAMRALHALKYTEEN
                     KLEKEIDEEKDTHKISRDSFLDFQRKTE"
BASE COUNT          310 a          174 c          152 g          390 t
ORIGIN      
        1 caaaaacttt caccatgtca ccttcttcag gatttccttc tatgctctga atagcacctg
       61 agaaaatcca cggatggaaa cgtttcacac tttcttcctt ttttggtttc aatcgcacag
      121 taatcataaa ataggaattg aattttaaaa tgaattaatt ggacccattt ttagcaggtg
      181 aattttccag tactaatttt tctatttctt ttttcgccaa cggttcttct ttcattaatt
      241 ggagatagat ttgcaaagtt gcccaagcat caatagcagc atattgtaat tgtttctcag
      301 tcagttgtgg tttttcccaa ttacttagtt tttgcgattt cagaattttt tctccgaaaa
      361 tgatagcata aattttctgc aagcttaatt ccataatgcc atagtttttt actatagatt
      421 gaatatcaat aaaattttca ggtttaaaat gatgacgttt ttttaatttt cttaaatcgt
      481 catgcagaga aagcccaact ttggtgatat ttttatcagc taaaaaatca gcaagagctt
      541 ttggaaaacc aattcgattt agtcggaata aaaaacattg ttcgggagta gcaatttgta
      601 aaagtgaaac tttatgggaa acgcctttga taaaagaagg ctttgtttct gtatcaattc
      661 ctactatatc atttttacga agttcagcaa tggacgattc tacttttgaa gattcatcta
      721 caacagtaat ttttcctgta aaagtagcaa cgggtaattt attaatttct tctttagata
      781 ttttaggtga atatttcatc ggttattatt ccgttttacg ttgaaaatca agaaacgaat
      841 ctcgactgat tttatgggta tctttttctt catctatttc tttttccaat ttattttctt
      901 ctgtatattt taaagcatga agtgctcgca ttgcatttaa taatttctgt ccccaatgtt
      961 ctgcaaaagt aaccagacaa gtaaccaacg catcattcat tatgtccaca tcagccagtt
     1021 gataat
//
LOCUS       DAOTKS010000443         1014 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_125508_length_1014_cov_2.887383, whole genome shotgun
            sequence.
ACCESSION   DAOTKS010000443 DAOTKS010000000
VERSION     DAOTKS010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125508_length_1014_cov_2.887383"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /gene="pepT"
                     /locus_tag="P5236_07175"
     CDS             complement(<1..925)
                     /gene="pepT"
                     /locus_tag="P5236_07175"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HRU64181.1"
                     /translation="MTTLVDRFLKYVEFTTTSDENTNLTPSTPGQLALAHYLVKELKS
                     IGLKDAEVDKNGYVMATLPANIDKEIPTIGFIAHLDTSPDMSGKNVKPKIIKNYDGKD
                     ILLNEEKLIVFETEKFPEILQYIGQDIIVTDGTTLLGADDKAGIAEIISAMEYLVDHP
                     EIKHGKIRIAFTPDEEIGKGVDYFNVKKFNAQWAYTIDGGEIGELQYENFNAASAKLT
                     FNGLNVHPGYARHKMKNSIRIAQQYMSMLPRHETPEHTEGYEGFYHITNIEGTVEKTT
                     LNCLIRDHDRDKFERRKKELQHLVNKINAEFG"
BASE COUNT          299 a          179 c          172 g          364 t
ORIGIN      
        1 cgccaaattc agcatttatt ttattgacaa gatgttgtaa ttcctttttt cttctttcga
       61 atttatcgcg atcatggtca cggataaggc agtttaacgt agttttttca acggtgcctt
      121 ctatattggt aatatggtaa aaaccttcat aaccttctgt atgttcgggc gtttcgtgtc
      181 ggggaagcat gctcatgtat tgttgagcaa tgcgaataga atttttcatt ttatgacggg
      241 cataacccgg atggacattc aaaccattaa aagtcaattt ggcagaagct gcattgaaat
      301 tttcatattg caattctcca atttctcctc catcgatggt ataagcccat tgtgcattaa
      361 atttttttac attgaaataa tctactcctt tcccgatttc ttcatcagga gtgaaagcaa
      421 tacggatttt tccatgttta atttcaggat ggtcaacaag atattccata gccgaaataa
      481 tttctgcaat tccagctttg tcatcagctc ctaacagcgt ggttccatca gtaacaataa
      541 tatcttgacc aatatattga agaatttcag gaaatttttc tgtttcaaaa acaatcagct
      601 tttcttcatt cagcagaata tctttcccat cataattttt gataattttt ggttttacat
      661 ttttgccact catatcgggg ctggtatcca gatgagcaat aaaaccaatg gtaggaattt
      721 ctttatcaat attggcaggt aaagtagcca ttacataacc atttttgtcc acttcagcat
      781 cttttagtcc tattgatttt aattctttta ccagataatg agcaagtgcc agttgaccag
      841 gtgtgctggg ggttaaattg gtgttttcat cagaagtagt ggtaaattct acatatttta
      901 aaaaacgatc tactaatgtt gtcatattgc tttcatttta tatgaataaa attaaacaat
      961 gcaaagttaa gataatattt ccttcaccga agatgatggg aagaaattca gggc
//
LOCUS       DAOTKS010000444         1011 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2967
            NODE_126079_length_1011_cov_4.559623, whole genome shotgun
            sequence.
ACCESSION   DAOTKS010000444 DAOTKS010000000
VERSION     DAOTKS010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867991
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 13:51:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,436
            CDSs (total)                      :: 1,415
            Genes (coding)                    :: 1,400
            CDSs (with protein)               :: 1,400
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 4 of 15
            Pseudo Genes (incomplete)         :: 6 of 15
            Pseudo Genes (internal stop)      :: 7 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126079_length_1011_cov_4.559623"
                     /isolate="AMR_MDS_2967"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..924
                     /locus_tag="P5236_07180"
     CDS             184..924
                     /locus_tag="P5236_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRU64182.1"
                     /translation="MNIFQDTGRYCILMEHVFIKPDRWKMFFRQFRNELDKLGLQSLP
                     IVLIISVFIGAVLTIQTKLNSENPLLPSYTTGLVTRDTLLLEFSSTIMCLILAGKVGS
                     NIASEIGTMRITEQIDALEIMGVNSANYLILPKIVAFVLMIPFLTIFSMAIGLIGGYF
                     VALFSDIITVAEYVTGIQYAFNAYYVFYSIVKSLVFAFLISSVSSYYGYYAYGGALDV
                     GKASTTAVVNSSILILFFNLLITNVMLK"
BASE COUNT          315 a          139 c          156 g          401 t
ORIGIN      
        1 tttttactaa atttcaaaaa ataactatat ttgtacactt ttcgataaag gaatctccat
       61 tcataaactt tctccaagaa cattttttga ctgttctttg agaagctttt ttaagttgta
      121 taaaaacttt gcaaaagata aggattaaaa aactctattt aacattaata acaaaaataa
      181 gaaatgaata tatttcaaga tacaggaaga tattgtatat tgatggaaca tgtttttatt
      241 aagcctgacc gttggaaaat gttttttcgt cagtttcgaa atgaattgga taaattagga
      301 ttacaatcat tacccattgt tttaattatt tcagtcttta ttggagctgt tttaactatt
      361 caaactaaat tgaattcgga aaatccatta ttaccttctt atactactgg tttagttacg
      421 cgtgatacct tgttgttaga attttcttct actattatgt gccttattct ggctgggaag
      481 gtcggttcta atattgcctc agaaattgga accatgcgaa ttaccgaaca aattgatgct
      541 ttggaaatta tgggtgtaaa ttctgccaat tatcttatcc ttcctaaaat tgtagctttt
      601 gttcttatga ttccttttct taccattttc agtatggcga taggtttaat tggaggctat
      661 tttgttgcac ttttcagtga cattattaca gtggctgaat atgttacagg aattcaatat
      721 gcttttaatg cttattatgt tttctattcc atagtcaaat cattggtttt tgcttttctt
      781 atttcttccg tttcttctta ctatggctat tatgcttatg gcggagcatt ggacgttgga
      841 aaagcaagca ctacagccgt agtgaacagc agcatattga tattattttt taacttattg
      901 attacaaatg tgatgttgaa ataatatttt tgcaaaaaac aattttttga aaattatggt
      961 gctatttgaa aaagcaaaaa tatttttacg attaactata aaatatgatt g
//
